×

MAGOH cDNA ORF clone, Homo sapiens (human)

Gene Symbol MAGOH
Entrez Gene ID 4116
Full Name mago homolog, exon junction complex core component
Synonyms MAGOH1, MAGOHA
General protein information
Preferred Names
protein mago nashi homolog
Names
protein mago nashi homolog
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p32.3

Summary Drosophila that have mutations in their mago nashi (grandchildless) gene produce progeny with defects in germplasm assembly and germline development. This gene encodes the mammalian mago nashi homolog. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

602603


mRNA and Protein(s)

mRNA Protein Name
NM_002370 NP_002361 protein mago nashi homolog

KEGG
hsa03040 Spliceosome
hsa03013 RNA transport
hsa03015 mRNA surveillance pathway
hsa_M00430 Exon junction complex (EJC)
Reactome
R-HSA-74160 Gene Expression
R-HSA-72202 Transport of Mature Transcript to Cytoplasm
R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72187 mRNA 3'-end processing
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-927802 Nonsense-Mediated Decay (NMD)
R-HSA-72172 mRNA Splicing
R-HSA-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)
R-HSA-73856 RNA Polymerase II Transcription Termination
R-HSA-109688 Cleavage of Growing Transcript in the Termination Region


Homo sapiens (human) MAGOH NP_002361.1
Pan troglodytes (chimpanzee) LOC100613301 XP_003308156.1
Macaca mulatta (Rhesus monkey) MAGOH XP_001112786.1
Canis lupus familiaris (dog) MAGOH XP_536702.1
Bos taurus (cattle) MAGOH NP_001029417.1
Mus musculus (house mouse) Magoh NP_001269666.1
Rattus norvegicus (Norway rat) Magoh NP_001094006.1
Gallus gallus (chicken) MAGOH2 NP_989656.1

Component

ID Name Evidence
GO:0005634 nucleus NAS
GO:0005654 nucleoplasm TAS
GO:0005737 cytoplasm IEA
GO:0005829 cytosol TAS
GO:0016607 nuclear speck IEA
GO:0035145 exon-exon junction complex IDA
GO:0071013 catalytic step 2 spliceosome IDA

Function

ID Name Evidence
GO:0003723 RNA binding IEA
GO:0005515 protein binding IPI

Process

ID Name Evidence
GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay IMP
GO:0000398 nuclear mRNA splicing, via spliceosome IC
GO:0000398 nuclear mRNA splicing, via spliceosome TAS
GO:0006366 transcription from RNA polymerase II promoter TAS
GO:0006369 termination of RNA polymerase II transcription TAS
GO:0006406 mRNA export from nucleus TAS
GO:0006417 regulation of translation IEA
GO:0006810 transport IEA
GO:0008380 RNA splicing EXP
GO:0010467 gene expression TAS
GO:0031124 mRNA 3'-end processing TAS

The following MAGOH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGOH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu17496
NM_002370 Homo sapiens mago homolog, exon junction complex core component (MAGOH), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $49.50
$99.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu17496
Clone ID Related Accession (Same CDS sequence) NM_002370
Accession Version NM_002370.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 441bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-JUL-2015
Organism Homo sapiens (human)
Product protein mago nashi homolog
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX283982.1, AF067173.1 and AF035940.1. On Jan 28, 2011 this sequence version replaced gi:6006021. Summary: Drosophila that have mutations in their mago nashi (grandchildless) gene produce progeny with defects in germplasm assembly and germline development. This gene encodes the mammalian mago nashi homolog. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF035940.1, BM808411.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
ATGGAGAGTG ACTTTTATCT GCGTTACTAC GTGGGGCACA AGGGCAAGTT CGGCCACGAG 
TTCCTGGAGT TTGAGTTTCG ACCGGACGGG AAGTTAAGAT ATGCCAACAA CAGCAATTAC
AAGAATGATG TCATGATCAG AAAAGAGGCT TATGTACATA AAAGCGTGAT GGAGGAACTG
AAGAGAATAA TTGACGACAG TGAAATTACC AAAGAGGATG ATGCATTGTG GCCTCCTCCT
GACCGAGTGG GCCGGCAGGA GCTTGAAATC GTCATTGGAG ATGAACACAT TTCTTTTACA
ACATCAAAAA TTGGTTCCCT TATTGATGTC AATCAATCCA AGGATCCAGA AGGCTTACGA
GTATTTTATT ATCTTGTCCA GGACCTGAAG TGTTTGGTCT TCAGTCTTAT TGGATTACAC
TTCAAGATTA AACCAATCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002361.1
CDS163..603
Misc Feature(1)61..63(+)
Misc Feature(2)163..165(+)
Misc Feature(3)172..600(+)
Misc Feature(4)193..600(+)
Misc Feature(5)193..597(+)
Misc Feature(6)199..471(+)
Misc Feature(7)202..585(+)
Misc Feature(8)247..588(+)
Misc Feature(9)358..483(+)
Exon (1)1..250
Gene:MAGOH
Gene Synonym:
Exon (2)251..309
Gene:MAGOH
Gene Synonym:
Exon (3)310..420
Gene:MAGOH
Gene Synonym:
Exon (4)421..503
Gene:MAGOH
Gene Synonym:
Exon (5)504..756
Gene:MAGOH
Gene Synonym:
Translation
Position Chain Variation Link
8 8 -, ccgggcgaagaggt dbSNP:140015267
11 11 g, t dbSNP:74686470
24 24 a, g dbSNP:568894728
34 34 a, g dbSNP:111645464
35 35 c, t dbSNP:765308630
57 57 a, g dbSNP:113851758
105 105 a, g dbSNP:558592853
112 112 a, g dbSNP:754085362
116 116 a, g dbSNP:766348180
118 118 a, c, g dbSNP:201700941
119 119 c, g dbSNP:566550176
127 127 c, t dbSNP:373348889
128 128 c, t dbSNP:751128432
130 130 -, tgg dbSNP:776284030
131 131 g, t dbSNP:369315204
132 132 -, g dbSNP:761482279
132 132 c, g dbSNP:762660187
138 138 c, g dbSNP:546730677
141 141 a, g, t dbSNP:536437660
144 144 c, t dbSNP:761033717
151 151 c, g dbSNP:375576053
156 156 c, g dbSNP:772657750
157 157 a, g dbSNP:748583764
195 195 c, g dbSNP:779093072
206 206 a, g dbSNP:112174103
237 237 a, g dbSNP:768856189
246 246 a, g dbSNP:749583136
273 273 c, t dbSNP:148806439
274 274 a, g dbSNP:199934311
276 276 c, t dbSNP:369761819
315 315 c, t dbSNP:764519975
325 325 a, g dbSNP:763619997
327 327 c, t dbSNP:776137567
344 344 a, c dbSNP:113912801
349 349 a, g dbSNP:765774176
357 357 c, t dbSNP:759547631
367 367 a, g dbSNP:776723649
372 372 c, g dbSNP:771089628
373 373 a, g dbSNP:747222044
386 386 c, t dbSNP:773037471
388 388 c, t dbSNP:368006653
407 407 a, g dbSNP:762974089
408 408 a, c dbSNP:748147829
415 415 c, t dbSNP:561502516
417 417 a, g dbSNP:768577043
432 432 a, c, t dbSNP:528481424
433 433 a, g dbSNP:749229462
436 436 a, g dbSNP:779490372
449 449 a, g dbSNP:145097978
450 450 c, t dbSNP:745315166
508 508 c, t dbSNP:367623791
543 543 a, g dbSNP:779201445
552 552 a, g dbSNP:755362534
564 564 c, t dbSNP:370758699
567 567 c, t dbSNP:745975060
591 591 g, t dbSNP:749545609
603 603 a, g dbSNP:200084732
606 606 -, t dbSNP:748336839
609 609 a, g dbSNP:780619732
610 610 c, t dbSNP:756560478
612 612 c, t dbSNP:750545788
616 616 c, t dbSNP:367804756
625 625 c, g dbSNP:757536909
629 629 a, g dbSNP:751803884
630 630 g, t dbSNP:764157200
631 631 g, t dbSNP:762822642
634 634 g, t dbSNP:775100128
635 635 g, t dbSNP:765296779
638 638 a, g dbSNP:199555808
641 641 a, t dbSNP:776329982
644 644 a, g dbSNP:770551129
646 646 a, c dbSNP:187097279
647 647 a, g dbSNP:772920165
650 650 g, t dbSNP:563888977
666 666 a, g dbSNP:540734079
677 677 a, g dbSNP:777193599
708 708 c, t dbSNP:527423382

Target ORF information:

RefSeq Version NM_002370
Organism Homo sapiens (human)
Definition Homo sapiens mago homolog, exon junction complex core component (MAGOH), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002370

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGAGAGTG ACTTTTATCT GCGTTACTAC GTGGGGCACA AGGGCAAGTT CGGCCACGAG 
TTCCTGGAGT TTGAGTTTCG ACCGGACGGG AAGTTAAGAT ATGCCAACAA CAGCAATTAC
AAGAATGATG TCATGATCAG AAAAGAGGCT TATGTACATA AAAGCGTGAT GGAGGAACTG
AAGAGAATAA TTGACGACAG TGAAATTACC AAAGAGGATG ATGCATTGTG GCCTCCTCCT
GACCGAGTGG GCCGGCAGGA GCTTGAAATC GTCATTGGAG ATGAACACAT TTCTTTTACA
ACATCAAAAA TTGGTTCCCT TATTGATGTC AATCAATCCA AGGATCCAGA AGGCTTACGA
GTATTTTATT ATCTTGTCCA GGACCTGAAG TGTTTGGTCT TCAGTCTTAT TGGATTACAC
TTCAAGATTA AACCAATCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A proteome-scale map of the human interactome network
Cell 159 (5), 1212-1226 (2014)
Rolland T, Tasan M, Charloteaux B, Pevzner SJ, Zhong Q, Sahni N, Yi S, Lemmens I, Fontanillo C, Mosca R, Kamburov A, Ghiassian SD, Yang X, Ghamsari L, Balcha D, Begg BE, Braun P, Brehme M, Broly MP, Carvunis AR, Convery-Zupan D, Corominas R, Coulombe-Huntington J, Dann E, Dreze M, Dricot A, Fan C, Franzosa E, Gebreab F, Gutierrez BJ, Hardy MF, Jin M, Kang S, Kiros R, Lin GN, Luck K, MacWilliams A, Menche J, Murray RR, Palagi A, Poulin MM, Rambout X, Rasla J, Reichert P, Romero V, Ruyssinck E, Sahalie JM, Scholz A, Shah AA, Sharma A, Shen Y, Spirohn K, Tam S, Tejeda AO, Trigg SA, Twizere JC, Vega K, Walsh J, Cusick ME, Xia Y, Barabasi AL, Iakoucheva LM, Aloy P, De Las Rivas J, Tavernier J, Calderwood MA, Hill DE, Hao T, Roth FP and Vidal M.


book

An atlas of genetic influences on human blood metabolites
Nat. Genet. 46 (6), 543-550 (2014)
Shin SY, Fauman EB, Petersen AK, Krumsiek J, Santos R, Huang J, Arnold M, Erte I, Forgetta V, Yang TP, Walter K, Menni C, Chen L, Vasquez L, Valdes AM, Hyde CL, Wang V, Ziemek D, Roberts P, Xi L, Grundberg E, Waldenberger M, Richards JB, Mohney RP, Milburn MV, John SL, Trimmer J, Theis FJ, Overington JP, Suhre K, Brosnan MJ, Gieger C, Kastenmuller G, Spector TD and Soranzo N.


book

Two mammalian MAGOH genes contribute to exon junction complex composition and nonsense-mediated decay
RNA Biol 10 (8), 1291-1298 (2013)
Singh KK, Wachsmuth L, Kulozik AE and Gehring NH.


book

Structural basis for the nuclear export activity of Importin13
EMBO J. 32 (6), 899-913 (2013)
Grunwald M, Lazzaretti D and Bono F.


book

The cellular EJC interactome reveals higher-order mRNP structure and an EJC-SR protein nexus
Cell 151 (4), 750-764 (2012)
Singh G, Kucukural A, Cenik C, Leszyk JD, Shaffer SA, Weng Z and Moore MJ.


book

Drosophila Y14 shuttles to the posterior of the oocyte and is required for oskar mRNA transport
Curr. Biol. 11 (21), 1666-1674 (2001)
Hachet O and Ephrussi A.


book

Importin 13: a novel mediator of nuclear import and export
EMBO J. 20 (14), 3685-3694 (2001)
Mingot JM, Kostka S, Kraft R, Hartmann E and Gorlich D.


book

MAGOH interacts with a novel RNA-binding protein
Genomics 63 (1), 145-148 (2000)
Zhao XF, Nowak NJ, Shows TB and Aplan PD.


book

The mammalian homologue of mago nashi encodes a serum-inducible protein
Genomics 47 (2), 319-322 (1998)
Zhao XF, Colaizzo-Anas T, Nowak NJ, Shows TB, Elliott RW and Aplan PD.


book

mago nashi mediates the posterior follicle cell-to-oocyte signal to organize axis formation in Drosophila
Development 124 (16), 3197-3207 (1997)
Newmark PA, Mohr SE, Gong L and Boswell RE.


 
*
*
*
*