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MAK male germ cell-associated kinase [Homo sapiens (human)]

Gene Symbol MAK
Entrez Gene ID 4117
Full Name male germ cell-associated kinase
Synonyms RP62, dJ417M14.2
General protein information
Preferred Names
serine/threonine-protein kinase MAK
Names
serine/threonine-protein kinase MAK
serine/threonine protein kinase MAK
male germ cell-associated kinase retinal-enriched isoform
NP_001229314.1
NP_001229886.1
NP_005897.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p24

Summary The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]. lac of sum
Disorder MIM:

154235

Disorder Html:





The following MAK gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAK gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18127 XM_011514619 PREDICTED: Homo sapiens male germ cell-associated kinase (MAK), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu18127 XM_011514620 PREDICTED: Homo sapiens male germ cell-associated kinase (MAK), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu58832 XM_011514621 PREDICTED: Homo sapiens male germ cell-associated kinase (MAK), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu20689 XM_011514622 PREDICTED: Homo sapiens male germ cell-associated kinase (MAK), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu58833 XM_011514623 PREDICTED: Homo sapiens male germ cell-associated kinase (MAK), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu58834 XM_011514624 PREDICTED: Homo sapiens male germ cell-associated kinase (MAK), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu20588 NM_005906 Homo sapiens male germ cell-associated kinase (MAK), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$439
OHu20689 NM_001242385 Homo sapiens male germ cell-associated kinase (MAK), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu18127 NM_001242957 Homo sapiens male germ cell-associated kinase (MAK), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu18127
Accession Version XM_011514619.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase MAK isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007592.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGAACCGAT ACACAACCAT GAGACAGTTG GGGGACGGCA CGTATGGGAG TGTGCTTATG 
GGCAAGAGTA ATGAATCCGG GGAGCTGGTG GCCATCAAAA GGATGAAGAG AAAGTTCTAT
TCTTGGGATG AATGCATGAA CTTGAGAGAA GTTAAGTCTC TGAAGAAACT TAATCATGCC
AATGTTATTA AATTGAAAGA AGTTATCAGA GAAAATGACC ATCTTTATTT TATATTTGAA
TATATGAAAG AAAACCTCTA TCAATTAATG AAAGACAGAA ACAAGTTGTT CCCTGAATCA
GTCATCAGAA ATATTATGTA TCAAATATTG CAAGGGCTGG CTTTTATCCA TAAACATGGC
TTTTTTCATA GGGACATGAA ACCAGAAAAC TTGCTTTGTA TGGGTCCAGA GCTTGTGAAA
ATTGCTGATT TTGGACTTGC AAGAGAATTA AGGTCACAGC CACCATACAC TGATTATGTA
TCTACCAGAT GGTATCGTGC CCCTGAAGTT TTACTGAGAT CTTCAGTTTA TAGTTCTCCC
ATTGATGTGT GGGCTGTTGG AAGTATCATG GCTGAACTCT ATATGTTAAG GCCACTTTTC
CCAGGGACAA GTGAGGTCGA TGAAATCTTT AAAATTTGCC AAGTTTTAGG GACTCCCAAA
AAAAGTGACT GGCCAGAAGG ATACCAGCTG GCATCCTCTA TGAACTTCCG TTTTCCCCAG
TGTGTTCCTA TAAACTTAAA AACTCTTATT CCCAATGCCA GTAATGAAGC TATTCAGCTC
ATGACCGAAA TGTTGAATTG GGATCCAAAG AAACGACCGA CAGCAAGCCA GGCATTGAAA
CACCCATATT TTCAAGTTGG TCAGGTATTA GGCCCTTCGT CAAATCATCT GGAATCAAAA
CAGTCTTTAA ATAAGCAGCT GCAACCATTA GAATCAAAGC CATCTTTAGT TGAGGTAGAG
CCTAAGCCTC TGCCGGATAT AATCGATCAG GTTGTTGGAC AACCCCAGCC AAAAACTAGC
CAGCAGCCAC TGCAGCCCAT TCAGCCGCCA CAGAACCTGA GCGTCCAGCA ACCTCCAAAG
CAACAGAGTC AGGAGAAACC GCCACAAACG CTATTCCCGA GCATCGTCAA AAACATGCCA
ACTAAGCCAA ATGGCACACT GAGTCATAAA AGTGGTAGGA GGCGTTGGGG TCAGACTATC
TTCAAGTCTG GAGATAGCTG GGAAGAGTTG GAGGACTATG ATTTCGGAGC CTCCCATTCC
AAGAAGCCAA GCATGGGTGT TTTTAAAGAA AAAAGGAAAA AAGATTCTCC ATTTCGGCTT
CCAGAGCCAG TACCCTCAGG CTCCAACCAC TCGACAGGGG AAAACAAGAG CTTACCTGCT
GTTACTTCCC TAAAATCTGA TTCCGAATTG TCAACTGCTC CAACCTCTAA ACAGTACTAC
TTGAAACAAT CAAGATATCT TCCAGGTGTG AATCCCAAGA AGGTGTCCTT GATAGCCAGT
GGAAAGGAAA TAAACCCCCA CACTTGGAGC AACCAGTTAT TCCCCAAGTC ACTGGGACCC
GTTGGGGCAG AACTTGCTTT CAAAAGGAGC AATGCAGAAG AAAGCATAAT TAAACCAATC
GAAAAACTAT CATGCAATGA AACTTTTCCT GAAAAATTAG AGGACCCACA AGGAAATCTT
GGAAGTTATG CTACTTACAA TCAGTCAGGA TATATTCCTT CCTTTCTCAA AAAAGAAGTG
CAGTCAGCTG GCCAGAGGAT CCACTTAGCA CCTCTCAATG CAACGGCTTC AGAATATACC
TGGAACACAA AAACTGGTCG GGGGCAGTTT TCAGGACGTA CTTATAATCC TACAGCAAAA
AACCTAAATA TTGTGAACCG TGCACAGCCC ATTCCCTCAG TGCATGGGAG GACAGACTGG
GTGGCCAAGT ATGGAGGCCA CCGGTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011512921.1
CDS369..2315
Misc Feature(1)378..1220(+)
Misc Feature(2)378..1220(+)
Misc Feature(3)396..974(+)
Misc Feature(4)396..797(+)
Misc Feature(5)513..974(+)
Misc Feature(6)792..860(+)
Translation
Position Chain Variation Link
25 25 a, c dbSNP:564096301
42 42 c, t dbSNP:552163500
54 54 a, c dbSNP:368011794
64 64 a, g dbSNP:749842220
114 114 c, t dbSNP:752301090
118 118 c, g dbSNP:564261
193 193 c, t dbSNP:546568347
210 210 a, g dbSNP:528500811
219 219 g, t dbSNP:570847928
245 245 c, t dbSNP:552689175
303 303 a, g dbSNP:181775528
323 323 a, c dbSNP:368252042
327 327 a, g dbSNP:781639259
337 337 a, g dbSNP:757503362
340 340 a, g dbSNP:375783146
347 347 a, t dbSNP:370663263
375 375 a, c, t dbSNP:558628181
376 376 a, g dbSNP:752676297
380 380 c, t dbSNP:765158321
384 384 a, g dbSNP:200406214
387 387 a, g dbSNP:753647340
396 396 c, t dbSNP:202067704
400 400 a, g dbSNP:760408768
404 404 c, t dbSNP:138352622
405 405 a, c, g dbSNP:387906647
409 409 c, t dbSNP:776197156
410 410 a, g dbSNP:770432926
414 414 a, g dbSNP:751370670
418 418 a, g dbSNP:777163256
419 419 c, t dbSNP:771149172
422 422 a, g dbSNP:747307777
425 425 c, t dbSNP:777830548
446 446 a, c, t dbSNP:542075413
447 447 a, c, g dbSNP:754916169
450 450 -, c dbSNP:35709746
458 458 a, g dbSNP:753737265
461 461 -, c dbSNP:753804603
477 477 a, g dbSNP:755003228
487 487 a, g dbSNP:560065834
493 493 g, t dbSNP:749261144
506 506 a, g dbSNP:779850347
509 509 c, g dbSNP:755994791
538 538 a, t dbSNP:568872972
540 540 a, g dbSNP:758042729
542 542 g, t dbSNP:371263215
552 552 a, g dbSNP:764738214
553 553 c, t dbSNP:761118698
561 561 c, t dbSNP:750895183
570 570 c, g dbSNP:767920227
583 583 a, g dbSNP:762158772
586 586 a, g dbSNP:774612770
590 590 c, t dbSNP:150892331
597 597 -, tttat dbSNP:781376741
600 600 a, g dbSNP:763168003
625 625 g, t dbSNP:775458619
626 626 -, ct dbSNP:757392221
636 636 a, c dbSNP:79404971
639 639 a, g dbSNP:769859859
653 653 a, g dbSNP:759659345
661 661 c, t dbSNP:776770214
663 663 c, g dbSNP:771009179
668 668 a, g dbSNP:746881510
678 678 a, g dbSNP:367716586
683 683 -, tat dbSNP:777554686
685 685 a, t dbSNP:773172706
686 686 a, g dbSNP:771852650
708 708 -, g dbSNP:527236082
724 724 a, g dbSNP:200506656
737 737 c, t dbSNP:200916721
739 739 a, g dbSNP:762987092
743 743 c, t dbSNP:773263219
756 756 a, c dbSNP:387906646
761 761 a, g dbSNP:771805362
762 762 -, cttc dbSNP:760171531
764 764 a, t dbSNP:747959498
768 768 a, g dbSNP:773956623
770 770 -, atg dbSNP:772837652
772 772 g, t dbSNP:768356424
773 773 g, t dbSNP:373994062
775 775 c, t dbSNP:779562217
779 779 a, g, t dbSNP:201804752
783 783 a, g dbSNP:747553199
789 789 a, g dbSNP:141420368
790 790 c, t dbSNP:758750076
803 803 a, g dbSNP:753121326
805 805 a, t dbSNP:765454168
807 807 a, g dbSNP:112561079
808 808 c, g dbSNP:755260124
818 818 a, g dbSNP:754068927
823 823 c, g dbSNP:766619005
832 832 c, g dbSNP:760809915
837 837 a, t dbSNP:375659310
844 844 a, g dbSNP:138795058
849 849 c, t dbSNP:372605229
852 852 a, t dbSNP:145065729
853 853 c, t dbSNP:774229391
856 856 a, g dbSNP:768283205
864 864 c, t dbSNP:527236081
865 865 a, g dbSNP:387906648
883 883 a, t dbSNP:373520122
886 886 c, g dbSNP:369612015
887 887 a, g dbSNP:748742307
888 888 a, t dbSNP:779260712
898 898 a, c dbSNP:769213821
899 899 c, t dbSNP:749659651
900 900 a, g dbSNP:572867029
904 904 c, t dbSNP:558155479
909 909 a, g dbSNP:747216229
910 910 c, t dbSNP:750559316
915 915 a, g dbSNP:781377439
917 917 a, g dbSNP:376610958
921 921 a, g dbSNP:527236080
924 924 g, t dbSNP:141312468
928 928 -, g dbSNP:770308128
933 933 a, g dbSNP:56215624
949 949 a, g dbSNP:752496358
951 951 -, at dbSNP:746288738
967 967 c, t dbSNP:369797377
974 974 a, g dbSNP:759210520
983 983 a, g dbSNP:138302657
986 986 c, t dbSNP:765957619
990 990 a, g dbSNP:760103192
1007 1007 a, c dbSNP:143572116
1014 1014 a, t dbSNP:769287766
1015 1015 c, t dbSNP:182178532
1042 1042 c, g dbSNP:112257972
1043 1043 a, g dbSNP:770348792
1046 1046 a, c dbSNP:375351078
1054 1054 a, t dbSNP:530738750
1057 1057 g, t dbSNP:771061280
1058 1058 a, g dbSNP:747215914
1068 1068 a, g dbSNP:754415973
1077 1077 c, t dbSNP:111618569
1078 1078 a, g dbSNP:746596336
1086 1086 c, t dbSNP:779514800
1087 1087 a, g dbSNP:201114107
1123 1123 a, g dbSNP:371639894
1124 1124 a, t dbSNP:377107543
1127 1127 c, t dbSNP:778820480
1135 1135 a, g dbSNP:754864509
1146 1146 a, c, g dbSNP:368102323
1154 1154 c, t dbSNP:199949446
1155 1155 a, g dbSNP:199689074
1166 1166 c, t dbSNP:767172193
1173 1173 c, t dbSNP:761403711
1182 1182 c, t dbSNP:753314164
1183 1183 a, g dbSNP:765675052
1186 1186 c, t dbSNP:149734152
1191 1191 a, g dbSNP:776892781
1203 1203 c, t dbSNP:756912445
1211 1211 c, t dbSNP:55950618
1219 1219 g, t dbSNP:763618857
1220 1220 g, t dbSNP:759984800
1230 1230 c, g dbSNP:754349931
1232 1232 a, g dbSNP:766693820
1235 1235 a, g dbSNP:147570352
1239 1239 a, g dbSNP:200901108
1246 1246 c, t dbSNP:773485283
1247 1247 a, g dbSNP:772299314
1249 1249 c, g dbSNP:761959677
1270 1270 a, t dbSNP:774449472
1278 1278 a, g dbSNP:199921765
1279 1279 a, t dbSNP:768757013
1287 1287 c, g dbSNP:186218889
1288 1288 g, t dbSNP:775490393
1297 1297 c, t dbSNP:769534346
1309 1309 c, g dbSNP:745670820
1315 1315 -, t dbSNP:777909571
1316 1316 a, c dbSNP:781065923
1317 1317 a, g dbSNP:757000356
1328 1328 c, g dbSNP:746612091
1335 1335 a, c dbSNP:762412793
1336 1336 c, g, t dbSNP:200641218
1342 1342 c, t dbSNP:371971492
1343 1343 a, g dbSNP:145014649
1344 1344 g, t dbSNP:367903138
1349 1349 a, c dbSNP:750794210
1352 1352 c, t dbSNP:767830843
1353 1353 a, g dbSNP:201812469
1355 1355 g, t dbSNP:17579447
1356 1356 c, t dbSNP:774598986
1362 1362 g, t dbSNP:764142857
1370 1370 -, acccca dbSNP:758224960
1373 1373 c, t dbSNP:763072144
1375 1375 -, a dbSNP:748139115
1379 1379 a, c dbSNP:775374397
1392 1392 c, g dbSNP:769859243
1403 1403 a, g dbSNP:139604404
1406 1406 c, g dbSNP:776588742
1408 1408 c, t dbSNP:770829149
1412 1412 c, g dbSNP:746701788
1414 1414 c, t dbSNP:371774117
1415 1415 a, g dbSNP:757905846
1417 1417 c, t dbSNP:747711453
1419 1419 c, t dbSNP:778223936
1421 1421 c, g dbSNP:756648303
1424 1424 c, g dbSNP:750884327
1428 1428 a, g dbSNP:767905978
1430 1430 c, t dbSNP:199694073
1431 1431 a, g dbSNP:751783623
1437 1437 c, t dbSNP:200300453
1439 1439 a, g dbSNP:143424442
1452 1452 -, ca dbSNP:755434575
1452 1452 c, g dbSNP:763018137
1461 1461 a, g dbSNP:201558986
1463 1463 a, g dbSNP:775668388
1466 1466 a, c dbSNP:765357899
1468 1468 -, cc dbSNP:141174473
1468 1468 c, g, t dbSNP:776680313
1469 1469 a, g dbSNP:770917055
1477 1477 c, t dbSNP:201494324
1478 1478 c, g dbSNP:772828352
1486 1486 c, t dbSNP:771764863
1489 1489 a, g dbSNP:747716951
1494 1494 a, g dbSNP:778510807
1496 1496 c, t dbSNP:768120333
1505 1505 c, g dbSNP:150744360
1507 1507 c, t dbSNP:781652966
1514 1514 a, g dbSNP:753029772
1519 1519 a, g dbSNP:55773478
1531 1531 c, g dbSNP:755195964
1544 1544 -, t dbSNP:753698504
1551 1551 c, t dbSNP:753974545
1552 1552 a, g, t dbSNP:62000445
1556 1556 c, g dbSNP:750310318
1559 1559 c, t dbSNP:551658772
1565 1565 a, t dbSNP:767409599
1573 1573 a, c dbSNP:200533678
1583 1583 c, t dbSNP:148549681
1588 1588 a, g dbSNP:763737966
1596 1596 c, t dbSNP:762551107
1597 1597 c, t dbSNP:144848825
1606 1606 a, g dbSNP:200944697
1613 1613 c, t dbSNP:115185609
1614 1614 a, g dbSNP:773588644
1615 1615 a, g dbSNP:549436795
1617 1617 g, t dbSNP:748539209
1618 1618 c, t dbSNP:779355214
1630 1630 a, g dbSNP:755285965
1634 1634 g, t dbSNP:137918175
1638 1638 a, c dbSNP:780233328
1645 1645 a, g dbSNP:139003494
1646 1646 c, t dbSNP:750428776
1656 1656 a, g dbSNP:767365219
1659 1659 a, t dbSNP:757149659
1664 1664 a, g dbSNP:751385674
1671 1671 g, t dbSNP:201502742
1673 1673 g, t dbSNP:760421859
1679 1679 a, g dbSNP:775091188
1683 1683 c, t dbSNP:764870230
1684 1684 a, g dbSNP:758956117
1685 1685 c, g dbSNP:762462087
1698 1698 c, g dbSNP:556048925
1699 1699 c, t dbSNP:769213984
1706 1706 a, c dbSNP:763420270
1709 1709 c, t dbSNP:775852553
1710 1710 c, t dbSNP:769938480
1715 1715 a, c dbSNP:746051642
1720 1720 c, t dbSNP:200057744
1722 1722 a, g dbSNP:193132857
1723 1723 a, c dbSNP:547372600
1724 1724 -, ag dbSNP:762837543
1727 1727 a, g dbSNP:747015059
1728 1728 -, g dbSNP:777144918
1734 1734 a, g dbSNP:777674662
1749 1749 a, g dbSNP:376120162
1750 1750 c, t dbSNP:146071625
1755 1755 a, g, t dbSNP:754612195
1772 1772 c, t dbSNP:184920692
1773 1773 a, g dbSNP:149006687
1780 1780 -, tgtc dbSNP:771209345
1782 1782 a, g dbSNP:760262193
1785 1785 a, g dbSNP:369794041
1787 1787 a, t dbSNP:749890675
1802 1802 a, g dbSNP:764699614
1804 1804 a, g dbSNP:763510409
1817 1817 a, g dbSNP:775738175
1821 1821 a, g dbSNP:770170708
1822 1822 g, t dbSNP:759716806
1828 1828 c, t dbSNP:776906266
1832 1832 a, g dbSNP:779438081
1836 1836 a, g dbSNP:776804120
1848 1848 -, aag dbSNP:375070639
1848 1848 a, g dbSNP:766593207
1849 1849 a, c, t dbSNP:773304876
1851 1851 g, t dbSNP:772119574
1853 1853 a, g dbSNP:748079104
1867 1867 -, g dbSNP:774717726
1885 1885 c, t dbSNP:752021255
1887 1887 -, c dbSNP:748216114
1889 1889 a, c, g dbSNP:375261960
1893 1893 c, t dbSNP:779951600
1898 1898 c, t dbSNP:755850716
1905 1905 g, t dbSNP:745490858
1926 1926 c, t dbSNP:567083
1928 1928 c, t dbSNP:756785609
1930 1930 c, t dbSNP:192591726
1936 1936 c, t dbSNP:765787091
1960 1960 a, c dbSNP:755373628
1961 1961 c, t dbSNP:754233012
1986 1986 a, g dbSNP:374286792
1988 1988 a, c dbSNP:745580809
1989 1989 a, g dbSNP:769238012
2005 2005 a, g dbSNP:371455796
2012 2012 c, t dbSNP:747805700
2039 2039 a, g dbSNP:552111002
2064 2064 c, t dbSNP:761680845
2066 2066 a, c dbSNP:186643840
2068 2068 a, g dbSNP:761897291
2081 2081 c, t dbSNP:751678907
2083 2083 c, t dbSNP:79544660
2085 2085 c, t dbSNP:371838435
2090 2090 c, t dbSNP:775561335
2091 2091 c, t dbSNP:56217305
2100 2100 a, c dbSNP:759417469
2101 2101 a, g dbSNP:776221542
2108 2108 a, g dbSNP:770648753
2109 2109 a, c dbSNP:746594889
2111 2111 c, g dbSNP:556910057
2123 2123 a, g dbSNP:111281539
2130 2130 c, t dbSNP:200319110
2143 2143 c, t dbSNP:771453698
2146 2146 a, g dbSNP:200866765
2153 2153 a, g dbSNP:143121053
2155 2155 c, t dbSNP:756558713
2156 2156 c, t dbSNP:142308835
2171 2171 a, g dbSNP:770320899
2180 2180 a, t dbSNP:757841911
2183 2183 a, c, t dbSNP:570463236
2186 2186 -, tcgg dbSNP:760121453
2187 2187 c, t dbSNP:199594233
2188 2188 a, g dbSNP:368535018
2202 2202 c, g dbSNP:201628941
2205 2205 c, t dbSNP:553591095
2206 2206 a, g dbSNP:758636733
2209 2209 c, t dbSNP:752846705
2215 2215 a, c dbSNP:371429892
2217 2217 c, g dbSNP:147251038
2218 2218 c, g dbSNP:753694997
2219 2219 c, t dbSNP:766252727
2223 2223 a, g dbSNP:760381134
2230 2230 a, c dbSNP:180826550
2231 2231 a, c dbSNP:767167233
2239 2239 g, t dbSNP:761401422
2243 2243 a, g dbSNP:773894456
2247 2247 c, t dbSNP:768134966
2248 2248 a, g, t dbSNP:777002916
2256 2256 c, g, t dbSNP:747349238
2263 2263 c, t dbSNP:778235109
2267 2267 -, ag dbSNP:754210226
2273 2273 c, t dbSNP:126405
2274 2274 a, g dbSNP:748349220
2276 2276 a, g dbSNP:537862624
2278 2278 a, g dbSNP:377372773
2289 2289 a, g dbSNP:567613995
2290 2290 g, t dbSNP:766229047
2310 2310 c, t dbSNP:375794836
2311 2311 a, c, g dbSNP:767273634
2322 2322 a, g dbSNP:370549111
2326 2326 g, t dbSNP:773982305
2327 2327 a, g dbSNP:377201252
2335 2335 -, ctac dbSNP:766869339
2348 2348 c, t dbSNP:372487961
2349 2349 c, t dbSNP:78074137
2350 2350 a, g dbSNP:376336149
2351 2351 a, t dbSNP:747511670
2352 2352 a, c dbSNP:773751765
2358 2358 c, t dbSNP:373416620
2365 2365 c, g dbSNP:369955868
2366 2366 c, t dbSNP:560376669
2415 2415 a, g dbSNP:373312456
2435 2435 c, g dbSNP:536214552
2449 2449 c, g dbSNP:552039665
2487 2487 c, g dbSNP:753480982
2503 2503 c, t dbSNP:3734395
2541 2541 a, g dbSNP:547465110
2593 2593 a, t dbSNP:544885539
2594 2594 a, c dbSNP:575950279
2598 2598 c, g dbSNP:200154458
2599 2599 c, t dbSNP:542247606
2607 2607 a, g dbSNP:753241358
2627 2627 -, a dbSNP:769006848
2646 2646 -, ta dbSNP:558307147
2692 2692 c, t dbSNP:572139433
2745 2745 c, t dbSNP:763523138
2779 2779 c, g dbSNP:553427676
2805 2805 c, t dbSNP:538465920
2901 2901 -, t dbSNP:397705840
2916 2916 -, t, tt dbSNP:60516370
2916 2916 g, t dbSNP:75280442
2917 2917 g, t dbSNP:76389200
2918 2918 a, g dbSNP:376366712
2922 2922 a, g dbSNP:577379885
2936 2936 c, g dbSNP:527434856

Target ORF information:

RefSeq Version XM_011514619
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens male germ cell-associated kinase (MAK), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011514619

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGAACCGAT ACACAACCAT GAGACAGTTG GGGGACGGCA CGTATGGGAG TGTGCTTATG 
GGCAAGAGTA ATGAATCCGG GGAGCTGGTG GCCATCAAAA GGATGAAGAG AAAGTTCTAT
TCTTGGGATG AATGCATGAA CTTGAGAGAA GTTAAGTCTC TGAAGAAACT TAATCATGCC
AATGTTATTA AATTGAAAGA AGTTATCAGA GAAAATGACC ATCTTTATTT TATATTTGAA
TATATGAAAG AAAACCTCTA TCAATTAATG AAAGACAGAA ACAAGTTGTT CCCTGAATCA
GTCATCAGAA ATATTATGTA TCAAATATTG CAAGGGCTGG CTTTTATCCA TAAACATGGC
TTTTTTCATA GGGACATGAA ACCAGAAAAC TTGCTTTGTA TGGGTCCAGA GCTTGTGAAA
ATTGCTGATT TTGGACTTGC AAGAGAATTA AGGTCACAGC CACCATACAC TGATTATGTA
TCTACCAGAT GGTATCGTGC CCCTGAAGTT TTACTGAGAT CTTCAGTTTA TAGTTCTCCC
ATTGATGTGT GGGCTGTTGG AAGTATCATG GCTGAACTCT ATATGTTAAG GCCACTTTTC
CCAGGGACAA GTGAGGTCGA TGAAATCTTT AAAATTTGCC AAGTTTTAGG GACTCCCAAA
AAAAGTGACT GGCCAGAAGG ATACCAGCTG GCATCCTCTA TGAACTTCCG TTTTCCCCAG
TGTGTTCCTA TAAACTTAAA AACTCTTATT CCCAATGCCA GTAATGAAGC TATTCAGCTC
ATGACCGAAA TGTTGAATTG GGATCCAAAG AAACGACCGA CAGCAAGCCA GGCATTGAAA
CACCCATATT TTCAAGTTGG TCAGGTATTA GGCCCTTCGT CAAATCATCT GGAATCAAAA
CAGTCTTTAA ATAAGCAGCT GCAACCATTA GAATCAAAGC CATCTTTAGT TGAGGTAGAG
CCTAAGCCTC TGCCGGATAT AATCGATCAG GTTGTTGGAC AACCCCAGCC AAAAACTAGC
CAGCAGCCAC TGCAGCCCAT TCAGCCGCCA CAGAACCTGA GCGTCCAGCA ACCTCCAAAG
CAACAGAGTC AGGAGAAACC GCCACAAACG CTATTCCCGA GCATCGTCAA AAACATGCCA
ACTAAGCCAA ATGGCACACT GAGTCATAAA AGTGGTAGGA GGCGTTGGGG TCAGACTATC
TTCAAGTCTG GAGATAGCTG GGAAGAGTTG GAGGACTATG ATTTCGGAGC CTCCCATTCC
AAGAAGCCAA GCATGGGTGT TTTTAAAGAA AAAAGGAAAA AAGATTCTCC ATTTCGGCTT
CCAGAGCCAG TACCCTCAGG CTCCAACCAC TCGACAGGGG AAAACAAGAG CTTACCTGCT
GTTACTTCCC TAAAATCTGA TTCCGAATTG TCAACTGCTC CAACCTCTAA ACAGTACTAC
TTGAAACAAT CAAGATATCT TCCAGGTGTG AATCCCAAGA AGGTGTCCTT GATAGCCAGT
GGAAAGGAAA TAAACCCCCA CACTTGGAGC AACCAGTTAT TCCCCAAGTC ACTGGGACCC
GTTGGGGCAG AACTTGCTTT CAAAAGGAGC AATGCAGAAG AAAGCATAAT TAAACCAATC
GAAAAACTAT CATGCAATGA AACTTTTCCT GAAAAATTAG AGGACCCACA AGGAAATCTT
GGAAGTTATG CTACTTACAA TCAGTCAGGA TATATTCCTT CCTTTCTCAA AAAAGAAGTG
CAGTCAGCTG GCCAGAGGAT CCACTTAGCA CCTCTCAATG CAACGGCTTC AGAATATACC
TGGAACACAA AAACTGGTCG GGGGCAGTTT TCAGGACGTA CTTATAATCC TACAGCAAAA
AACCTAAATA TTGTGAACCG TGCACAGCCC ATTCCCTCAG TGCATGGGAG GACAGACTGG
GTGGCCAAGT ATGGAGGCCA CCGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18127
Accession Version XM_011514620.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase MAK isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007592.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGAACCGAT ACACAACCAT GAGACAGTTG GGGGACGGCA CGTATGGGAG TGTGCTTATG 
GGCAAGAGTA ATGAATCCGG GGAGCTGGTG GCCATCAAAA GGATGAAGAG AAAGTTCTAT
TCTTGGGATG AATGCATGAA CTTGAGAGAA GTTAAGTCTC TGAAGAAACT TAATCATGCC
AATGTTATTA AATTGAAAGA AGTTATCAGA GAAAATGACC ATCTTTATTT TATATTTGAA
TATATGAAAG AAAACCTCTA TCAATTAATG AAAGACAGAA ACAAGTTGTT CCCTGAATCA
GTCATCAGAA ATATTATGTA TCAAATATTG CAAGGGCTGG CTTTTATCCA TAAACATGGC
TTTTTTCATA GGGACATGAA ACCAGAAAAC TTGCTTTGTA TGGGTCCAGA GCTTGTGAAA
ATTGCTGATT TTGGACTTGC AAGAGAATTA AGGTCACAGC CACCATACAC TGATTATGTA
TCTACCAGAT GGTATCGTGC CCCTGAAGTT TTACTGAGAT CTTCAGTTTA TAGTTCTCCC
ATTGATGTGT GGGCTGTTGG AAGTATCATG GCTGAACTCT ATATGTTAAG GCCACTTTTC
CCAGGGACAA GTGAGGTCGA TGAAATCTTT AAAATTTGCC AAGTTTTAGG GACTCCCAAA
AAAAGTGACT GGCCAGAAGG ATACCAGCTG GCATCCTCTA TGAACTTCCG TTTTCCCCAG
TGTGTTCCTA TAAACTTAAA AACTCTTATT CCCAATGCCA GTAATGAAGC TATTCAGCTC
ATGACCGAAA TGTTGAATTG GGATCCAAAG AAACGACCGA CAGCAAGCCA GGCATTGAAA
CACCCATATT TTCAAGTTGG TCAGGTATTA GGCCCTTCGT CAAATCATCT GGAATCAAAA
CAGTCTTTAA ATAAGCAGCT GCAACCATTA GAATCAAAGC CATCTTTAGT TGAGGTAGAG
CCTAAGCCTC TGCCGGATAT AATCGATCAG GTTGTTGGAC AACCCCAGCC AAAAACTAGC
CAGCAGCCAC TGCAGCCCAT TCAGCCGCCA CAGAACCTGA GCGTCCAGCA ACCTCCAAAG
CAACAGAGTC AGGAGAAACC GCCACAAACG CTATTCCCGA GCATCGTCAA AAACATGCCA
ACTAAGCCAA ATGGCACACT GAGTCATAAA AGTGGTAGGA GGCGTTGGGG TCAGACTATC
TTCAAGTCTG GAGATAGCTG GGAAGAGTTG GAGGACTATG ATTTCGGAGC CTCCCATTCC
AAGAAGCCAA GCATGGGTGT TTTTAAAGAA AAAAGGAAAA AAGATTCTCC ATTTCGGCTT
CCAGAGCCAG TACCCTCAGG CTCCAACCAC TCGACAGGGG AAAACAAGAG CTTACCTGCT
GTTACTTCCC TAAAATCTGA TTCCGAATTG TCAACTGCTC CAACCTCTAA ACAGTACTAC
TTGAAACAAT CAAGATATCT TCCAGGTGTG AATCCCAAGA AGGTGTCCTT GATAGCCAGT
GGAAAGGAAA TAAACCCCCA CACTTGGAGC AACCAGTTAT TCCCCAAGTC ACTGGGACCC
GTTGGGGCAG AACTTGCTTT CAAAAGGAGC AATGCAGAAG AAAGCATAAT TAAACCAATC
GAAAAACTAT CATGCAATGA AACTTTTCCT GAAAAATTAG AGGACCCACA AGGAAATCTT
GGAAGTTATG CTACTTACAA TCAGTCAGGA TATATTCCTT CCTTTCTCAA AAAAGAAGTG
CAGTCAGCTG GCCAGAGGAT CCACTTAGCA CCTCTCAATG CAACGGCTTC AGAATATACC
TGGAACACAA AAACTGGTCG GGGGCAGTTT TCAGGACGTA CTTATAATCC TACAGCAAAA
AACCTAAATA TTGTGAACCG TGCACAGCCC ATTCCCTCAG TGCATGGGAG GACAGACTGG
GTGGCCAAGT ATGGAGGCCA CCGGTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011512922.1
CDS251..2197
Misc Feature(1)260..1102(+)
Misc Feature(2)260..1102(+)
Misc Feature(3)278..856(+)
Misc Feature(4)278..679(+)
Misc Feature(5)395..856(+)
Misc Feature(6)674..742(+)
Translation
Position Chain Variation Link
75 75 c, t dbSNP:546568347
92 92 a, g dbSNP:528500811
101 101 g, t dbSNP:570847928
127 127 c, t dbSNP:552689175
185 185 a, g dbSNP:181775528
205 205 a, c dbSNP:368252042
209 209 a, g dbSNP:781639259
219 219 a, g dbSNP:757503362
222 222 a, g dbSNP:375783146
229 229 a, t dbSNP:370663263
257 257 a, c, t dbSNP:558628181
258 258 a, g dbSNP:752676297
262 262 c, t dbSNP:765158321
266 266 a, g dbSNP:200406214
269 269 a, g dbSNP:753647340
278 278 c, t dbSNP:202067704
282 282 a, g dbSNP:760408768
286 286 c, t dbSNP:138352622
287 287 a, c, g dbSNP:387906647
291 291 c, t dbSNP:776197156
292 292 a, g dbSNP:770432926
296 296 a, g dbSNP:751370670
300 300 a, g dbSNP:777163256
301 301 c, t dbSNP:771149172
304 304 a, g dbSNP:747307777
307 307 c, t dbSNP:777830548
328 328 a, c, t dbSNP:542075413
329 329 a, c, g dbSNP:754916169
332 332 -, c dbSNP:35709746
340 340 a, g dbSNP:753737265
343 343 -, c dbSNP:753804603
359 359 a, g dbSNP:755003228
369 369 a, g dbSNP:560065834
375 375 g, t dbSNP:749261144
388 388 a, g dbSNP:779850347
391 391 c, g dbSNP:755994791
420 420 a, t dbSNP:568872972
422 422 a, g dbSNP:758042729
424 424 g, t dbSNP:371263215
434 434 a, g dbSNP:764738214
435 435 c, t dbSNP:761118698
443 443 c, t dbSNP:750895183
452 452 c, g dbSNP:767920227
465 465 a, g dbSNP:762158772
468 468 a, g dbSNP:774612770
472 472 c, t dbSNP:150892331
479 479 -, tttat dbSNP:781376741
482 482 a, g dbSNP:763168003
507 507 g, t dbSNP:775458619
508 508 -, ct dbSNP:757392221
518 518 a, c dbSNP:79404971
521 521 a, g dbSNP:769859859
535 535 a, g dbSNP:759659345
543 543 c, t dbSNP:776770214
545 545 c, g dbSNP:771009179
550 550 a, g dbSNP:746881510
560 560 a, g dbSNP:367716586
565 565 -, tat dbSNP:777554686
567 567 a, t dbSNP:773172706
568 568 a, g dbSNP:771852650
590 590 -, g dbSNP:527236082
606 606 a, g dbSNP:200506656
619 619 c, t dbSNP:200916721
621 621 a, g dbSNP:762987092
625 625 c, t dbSNP:773263219
638 638 a, c dbSNP:387906646
643 643 a, g dbSNP:771805362
644 644 -, cttc dbSNP:760171531
646 646 a, t dbSNP:747959498
650 650 a, g dbSNP:773956623
652 652 -, atg dbSNP:772837652
654 654 g, t dbSNP:768356424
655 655 g, t dbSNP:373994062
657 657 c, t dbSNP:779562217
661 661 a, g, t dbSNP:201804752
665 665 a, g dbSNP:747553199
671 671 a, g dbSNP:141420368
672 672 c, t dbSNP:758750076
685 685 a, g dbSNP:753121326
687 687 a, t dbSNP:765454168
689 689 a, g dbSNP:112561079
690 690 c, g dbSNP:755260124
700 700 a, g dbSNP:754068927
705 705 c, g dbSNP:766619005
714 714 c, g dbSNP:760809915
719 719 a, t dbSNP:375659310
726 726 a, g dbSNP:138795058
731 731 c, t dbSNP:372605229
734 734 a, t dbSNP:145065729
735 735 c, t dbSNP:774229391
738 738 a, g dbSNP:768283205
746 746 c, t dbSNP:527236081
747 747 a, g dbSNP:387906648
765 765 a, t dbSNP:373520122
768 768 c, g dbSNP:369612015
769 769 a, g dbSNP:748742307
770 770 a, t dbSNP:779260712
780 780 a, c dbSNP:769213821
781 781 c, t dbSNP:749659651
782 782 a, g dbSNP:572867029
786 786 c, t dbSNP:558155479
791 791 a, g dbSNP:747216229
792 792 c, t dbSNP:750559316
797 797 a, g dbSNP:781377439
799 799 a, g dbSNP:376610958
803 803 a, g dbSNP:527236080
806 806 g, t dbSNP:141312468
810 810 -, g dbSNP:770308128
815 815 a, g dbSNP:56215624
831 831 a, g dbSNP:752496358
833 833 -, at dbSNP:746288738
849 849 c, t dbSNP:369797377
856 856 a, g dbSNP:759210520
865 865 a, g dbSNP:138302657
868 868 c, t dbSNP:765957619
872 872 a, g dbSNP:760103192
889 889 a, c dbSNP:143572116
896 896 a, t dbSNP:769287766
897 897 c, t dbSNP:182178532
924 924 c, g dbSNP:112257972
925 925 a, g dbSNP:770348792
928 928 a, c dbSNP:375351078
936 936 a, t dbSNP:530738750
939 939 g, t dbSNP:771061280
940 940 a, g dbSNP:747215914
950 950 a, g dbSNP:754415973
959 959 c, t dbSNP:111618569
960 960 a, g dbSNP:746596336
968 968 c, t dbSNP:779514800
969 969 a, g dbSNP:201114107
1005 1005 a, g dbSNP:371639894
1006 1006 a, t dbSNP:377107543
1009 1009 c, t dbSNP:778820480
1017 1017 a, g dbSNP:754864509
1028 1028 a, c, g dbSNP:368102323
1036 1036 c, t dbSNP:199949446
1037 1037 a, g dbSNP:199689074
1048 1048 c, t dbSNP:767172193
1055 1055 c, t dbSNP:761403711
1064 1064 c, t dbSNP:753314164
1065 1065 a, g dbSNP:765675052
1068 1068 c, t dbSNP:149734152
1073 1073 a, g dbSNP:776892781
1085 1085 c, t dbSNP:756912445
1093 1093 c, t dbSNP:55950618
1101 1101 g, t dbSNP:763618857
1102 1102 g, t dbSNP:759984800
1112 1112 c, g dbSNP:754349931
1114 1114 a, g dbSNP:766693820
1117 1117 a, g dbSNP:147570352
1121 1121 a, g dbSNP:200901108
1128 1128 c, t dbSNP:773485283
1129 1129 a, g dbSNP:772299314
1131 1131 c, g dbSNP:761959677
1152 1152 a, t dbSNP:774449472
1160 1160 a, g dbSNP:199921765
1161 1161 a, t dbSNP:768757013
1169 1169 c, g dbSNP:186218889
1170 1170 g, t dbSNP:775490393
1179 1179 c, t dbSNP:769534346
1191 1191 c, g dbSNP:745670820
1197 1197 -, t dbSNP:777909571
1198 1198 a, c dbSNP:781065923
1199 1199 a, g dbSNP:757000356
1210 1210 c, g dbSNP:746612091
1217 1217 a, c dbSNP:762412793
1218 1218 c, g, t dbSNP:200641218
1224 1224 c, t dbSNP:371971492
1225 1225 a, g dbSNP:145014649
1226 1226 g, t dbSNP:367903138
1231 1231 a, c dbSNP:750794210
1234 1234 c, t dbSNP:767830843
1235 1235 a, g dbSNP:201812469
1237 1237 g, t dbSNP:17579447
1238 1238 c, t dbSNP:774598986
1244 1244 g, t dbSNP:764142857
1252 1252 -, acccca dbSNP:758224960
1255 1255 c, t dbSNP:763072144
1257 1257 -, a dbSNP:748139115
1261 1261 a, c dbSNP:775374397
1274 1274 c, g dbSNP:769859243
1285 1285 a, g dbSNP:139604404
1288 1288 c, g dbSNP:776588742
1290 1290 c, t dbSNP:770829149
1294 1294 c, g dbSNP:746701788
1296 1296 c, t dbSNP:371774117
1297 1297 a, g dbSNP:757905846
1299 1299 c, t dbSNP:747711453
1301 1301 c, t dbSNP:778223936
1303 1303 c, g dbSNP:756648303
1306 1306 c, g dbSNP:750884327
1310 1310 a, g dbSNP:767905978
1312 1312 c, t dbSNP:199694073
1313 1313 a, g dbSNP:751783623
1319 1319 c, t dbSNP:200300453
1321 1321 a, g dbSNP:143424442
1334 1334 -, ca dbSNP:755434575
1334 1334 c, g dbSNP:763018137
1343 1343 a, g dbSNP:201558986
1345 1345 a, g dbSNP:775668388
1348 1348 a, c dbSNP:765357899
1350 1350 -, cc dbSNP:141174473
1350 1350 c, g, t dbSNP:776680313
1351 1351 a, g dbSNP:770917055
1359 1359 c, t dbSNP:201494324
1360 1360 c, g dbSNP:772828352
1368 1368 c, t dbSNP:771764863
1371 1371 a, g dbSNP:747716951
1376 1376 a, g dbSNP:778510807
1378 1378 c, t dbSNP:768120333
1387 1387 c, g dbSNP:150744360
1389 1389 c, t dbSNP:781652966
1396 1396 a, g dbSNP:753029772
1401 1401 a, g dbSNP:55773478
1413 1413 c, g dbSNP:755195964
1426 1426 -, t dbSNP:753698504
1433 1433 c, t dbSNP:753974545
1434 1434 a, g, t dbSNP:62000445
1438 1438 c, g dbSNP:750310318
1441 1441 c, t dbSNP:551658772
1447 1447 a, t dbSNP:767409599
1455 1455 a, c dbSNP:200533678
1465 1465 c, t dbSNP:148549681
1470 1470 a, g dbSNP:763737966
1478 1478 c, t dbSNP:762551107
1479 1479 c, t dbSNP:144848825
1488 1488 a, g dbSNP:200944697
1495 1495 c, t dbSNP:115185609
1496 1496 a, g dbSNP:773588644
1497 1497 a, g dbSNP:549436795
1499 1499 g, t dbSNP:748539209
1500 1500 c, t dbSNP:779355214
1512 1512 a, g dbSNP:755285965
1516 1516 g, t dbSNP:137918175
1520 1520 a, c dbSNP:780233328
1527 1527 a, g dbSNP:139003494
1528 1528 c, t dbSNP:750428776
1538 1538 a, g dbSNP:767365219
1541 1541 a, t dbSNP:757149659
1546 1546 a, g dbSNP:751385674
1553 1553 g, t dbSNP:201502742
1555 1555 g, t dbSNP:760421859
1561 1561 a, g dbSNP:775091188
1565 1565 c, t dbSNP:764870230
1566 1566 a, g dbSNP:758956117
1567 1567 c, g dbSNP:762462087
1580 1580 c, g dbSNP:556048925
1581 1581 c, t dbSNP:769213984
1588 1588 a, c dbSNP:763420270
1591 1591 c, t dbSNP:775852553
1592 1592 c, t dbSNP:769938480
1597 1597 a, c dbSNP:746051642
1602 1602 c, t dbSNP:200057744
1604 1604 a, g dbSNP:193132857
1605 1605 a, c dbSNP:547372600
1606 1606 -, ag dbSNP:762837543
1609 1609 a, g dbSNP:747015059
1610 1610 -, g dbSNP:777144918
1616 1616 a, g dbSNP:777674662
1631 1631 a, g dbSNP:376120162
1632 1632 c, t dbSNP:146071625
1637 1637 a, g, t dbSNP:754612195
1654 1654 c, t dbSNP:184920692
1655 1655 a, g dbSNP:149006687
1662 1662 -, tgtc dbSNP:771209345
1664 1664 a, g dbSNP:760262193
1667 1667 a, g dbSNP:369794041
1669 1669 a, t dbSNP:749890675
1684 1684 a, g dbSNP:764699614
1686 1686 a, g dbSNP:763510409
1699 1699 a, g dbSNP:775738175
1703 1703 a, g dbSNP:770170708
1704 1704 g, t dbSNP:759716806
1710 1710 c, t dbSNP:776906266
1714 1714 a, g dbSNP:779438081
1718 1718 a, g dbSNP:776804120
1730 1730 -, aag dbSNP:375070639
1730 1730 a, g dbSNP:766593207
1731 1731 a, c, t dbSNP:773304876
1733 1733 g, t dbSNP:772119574
1735 1735 a, g dbSNP:748079104
1749 1749 -, g dbSNP:774717726
1767 1767 c, t dbSNP:752021255
1769 1769 -, c dbSNP:748216114
1771 1771 a, c, g dbSNP:375261960
1775 1775 c, t dbSNP:779951600
1780 1780 c, t dbSNP:755850716
1787 1787 g, t dbSNP:745490858
1808 1808 c, t dbSNP:567083
1810 1810 c, t dbSNP:756785609
1812 1812 c, t dbSNP:192591726
1818 1818 c, t dbSNP:765787091
1842 1842 a, c dbSNP:755373628
1843 1843 c, t dbSNP:754233012
1868 1868 a, g dbSNP:374286792
1870 1870 a, c dbSNP:745580809
1871 1871 a, g dbSNP:769238012
1887 1887 a, g dbSNP:371455796
1894 1894 c, t dbSNP:747805700
1921 1921 a, g dbSNP:552111002
1946 1946 c, t dbSNP:761680845
1948 1948 a, c dbSNP:186643840
1950 1950 a, g dbSNP:761897291
1963 1963 c, t dbSNP:751678907
1965 1965 c, t dbSNP:79544660
1967 1967 c, t dbSNP:371838435
1972 1972 c, t dbSNP:775561335
1973 1973 c, t dbSNP:56217305
1982 1982 a, c dbSNP:759417469
1983 1983 a, g dbSNP:776221542
1990 1990 a, g dbSNP:770648753
1991 1991 a, c dbSNP:746594889
1993 1993 c, g dbSNP:556910057
2005 2005 a, g dbSNP:111281539
2012 2012 c, t dbSNP:200319110
2025 2025 c, t dbSNP:771453698
2028 2028 a, g dbSNP:200866765
2035 2035 a, g dbSNP:143121053
2037 2037 c, t dbSNP:756558713
2038 2038 c, t dbSNP:142308835
2053 2053 a, g dbSNP:770320899
2062 2062 a, t dbSNP:757841911
2065 2065 a, c, t dbSNP:570463236
2068 2068 -, tcgg dbSNP:760121453
2069 2069 c, t dbSNP:199594233
2070 2070 a, g dbSNP:368535018
2084 2084 c, g dbSNP:201628941
2087 2087 c, t dbSNP:553591095
2088 2088 a, g dbSNP:758636733
2091 2091 c, t dbSNP:752846705
2097 2097 a, c dbSNP:371429892
2099 2099 c, g dbSNP:147251038
2100 2100 c, g dbSNP:753694997
2101 2101 c, t dbSNP:766252727
2105 2105 a, g dbSNP:760381134
2112 2112 a, c dbSNP:180826550
2113 2113 a, c dbSNP:767167233
2121 2121 g, t dbSNP:761401422
2125 2125 a, g dbSNP:773894456
2129 2129 c, t dbSNP:768134966
2130 2130 a, g, t dbSNP:777002916
2138 2138 c, g, t dbSNP:747349238
2145 2145 c, t dbSNP:778235109
2149 2149 -, ag dbSNP:754210226
2155 2155 c, t dbSNP:126405
2156 2156 a, g dbSNP:748349220
2158 2158 a, g dbSNP:537862624
2160 2160 a, g dbSNP:377372773
2171 2171 a, g dbSNP:567613995
2172 2172 g, t dbSNP:766229047
2192 2192 c, t dbSNP:375794836
2193 2193 a, c, g dbSNP:767273634
2204 2204 a, g dbSNP:370549111
2208 2208 g, t dbSNP:773982305
2209 2209 a, g dbSNP:377201252
2217 2217 -, ctac dbSNP:766869339
2230 2230 c, t dbSNP:372487961
2231 2231 c, t dbSNP:78074137
2232 2232 a, g dbSNP:376336149
2233 2233 a, t dbSNP:747511670
2234 2234 a, c dbSNP:773751765
2240 2240 c, t dbSNP:373416620
2247 2247 c, g dbSNP:369955868
2248 2248 c, t dbSNP:560376669
2297 2297 a, g dbSNP:373312456
2317 2317 c, g dbSNP:536214552
2331 2331 c, g dbSNP:552039665
2369 2369 c, g dbSNP:753480982
2385 2385 c, t dbSNP:3734395
2423 2423 a, g dbSNP:547465110
2475 2475 a, t dbSNP:544885539
2476 2476 a, c dbSNP:575950279
2480 2480 c, g dbSNP:200154458
2481 2481 c, t dbSNP:542247606
2489 2489 a, g dbSNP:753241358
2509 2509 -, a dbSNP:769006848
2528 2528 -, ta dbSNP:558307147
2574 2574 c, t dbSNP:572139433
2627 2627 c, t dbSNP:763523138
2661 2661 c, g dbSNP:553427676
2687 2687 c, t dbSNP:538465920
2783 2783 -, t dbSNP:397705840
2798 2798 -, t, tt dbSNP:60516370
2798 2798 g, t dbSNP:75280442
2799 2799 g, t dbSNP:76389200
2800 2800 a, g dbSNP:376366712
2804 2804 a, g dbSNP:577379885
2818 2818 c, g dbSNP:527434856

Target ORF information:

RefSeq Version XM_011514620
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens male germ cell-associated kinase (MAK), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011514620

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGAACCGAT ACACAACCAT GAGACAGTTG GGGGACGGCA CGTATGGGAG TGTGCTTATG 
GGCAAGAGTA ATGAATCCGG GGAGCTGGTG GCCATCAAAA GGATGAAGAG AAAGTTCTAT
TCTTGGGATG AATGCATGAA CTTGAGAGAA GTTAAGTCTC TGAAGAAACT TAATCATGCC
AATGTTATTA AATTGAAAGA AGTTATCAGA GAAAATGACC ATCTTTATTT TATATTTGAA
TATATGAAAG AAAACCTCTA TCAATTAATG AAAGACAGAA ACAAGTTGTT CCCTGAATCA
GTCATCAGAA ATATTATGTA TCAAATATTG CAAGGGCTGG CTTTTATCCA TAAACATGGC
TTTTTTCATA GGGACATGAA ACCAGAAAAC TTGCTTTGTA TGGGTCCAGA GCTTGTGAAA
ATTGCTGATT TTGGACTTGC AAGAGAATTA AGGTCACAGC CACCATACAC TGATTATGTA
TCTACCAGAT GGTATCGTGC CCCTGAAGTT TTACTGAGAT CTTCAGTTTA TAGTTCTCCC
ATTGATGTGT GGGCTGTTGG AAGTATCATG GCTGAACTCT ATATGTTAAG GCCACTTTTC
CCAGGGACAA GTGAGGTCGA TGAAATCTTT AAAATTTGCC AAGTTTTAGG GACTCCCAAA
AAAAGTGACT GGCCAGAAGG ATACCAGCTG GCATCCTCTA TGAACTTCCG TTTTCCCCAG
TGTGTTCCTA TAAACTTAAA AACTCTTATT CCCAATGCCA GTAATGAAGC TATTCAGCTC
ATGACCGAAA TGTTGAATTG GGATCCAAAG AAACGACCGA CAGCAAGCCA GGCATTGAAA
CACCCATATT TTCAAGTTGG TCAGGTATTA GGCCCTTCGT CAAATCATCT GGAATCAAAA
CAGTCTTTAA ATAAGCAGCT GCAACCATTA GAATCAAAGC CATCTTTAGT TGAGGTAGAG
CCTAAGCCTC TGCCGGATAT AATCGATCAG GTTGTTGGAC AACCCCAGCC AAAAACTAGC
CAGCAGCCAC TGCAGCCCAT TCAGCCGCCA CAGAACCTGA GCGTCCAGCA ACCTCCAAAG
CAACAGAGTC AGGAGAAACC GCCACAAACG CTATTCCCGA GCATCGTCAA AAACATGCCA
ACTAAGCCAA ATGGCACACT GAGTCATAAA AGTGGTAGGA GGCGTTGGGG TCAGACTATC
TTCAAGTCTG GAGATAGCTG GGAAGAGTTG GAGGACTATG ATTTCGGAGC CTCCCATTCC
AAGAAGCCAA GCATGGGTGT TTTTAAAGAA AAAAGGAAAA AAGATTCTCC ATTTCGGCTT
CCAGAGCCAG TACCCTCAGG CTCCAACCAC TCGACAGGGG AAAACAAGAG CTTACCTGCT
GTTACTTCCC TAAAATCTGA TTCCGAATTG TCAACTGCTC CAACCTCTAA ACAGTACTAC
TTGAAACAAT CAAGATATCT TCCAGGTGTG AATCCCAAGA AGGTGTCCTT GATAGCCAGT
GGAAAGGAAA TAAACCCCCA CACTTGGAGC AACCAGTTAT TCCCCAAGTC ACTGGGACCC
GTTGGGGCAG AACTTGCTTT CAAAAGGAGC AATGCAGAAG AAAGCATAAT TAAACCAATC
GAAAAACTAT CATGCAATGA AACTTTTCCT GAAAAATTAG AGGACCCACA AGGAAATCTT
GGAAGTTATG CTACTTACAA TCAGTCAGGA TATATTCCTT CCTTTCTCAA AAAAGAAGTG
CAGTCAGCTG GCCAGAGGAT CCACTTAGCA CCTCTCAATG CAACGGCTTC AGAATATACC
TGGAACACAA AAACTGGTCG GGGGCAGTTT TCAGGACGTA CTTATAATCC TACAGCAAAA
AACCTAAATA TTGTGAACCG TGCACAGCCC ATTCCCTCAG TGCATGGGAG GACAGACTGG
GTGGCCAAGT ATGGAGGCCA CCGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58832
Accession Version XM_011514621.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1890bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase MAK isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007592.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGAAACACA CAAGACGCCG GAGCTTCACA AGGGAGCGAA CGAGGATGAA GAGAAAGTTC 
TATTCTTGGG ATGAATGCAT GAACTTGAGA GAAGTTAAGT CTCTGAAGAA ACTTAATCAT
GCCAATGTTA TTAAATTGAA AGAAGTTATC AGAGAAAATG ACCATCTTTA TTTTATATTT
GAATATATGA AAGAAAACCT CTATCAATTA ATGAAAGACA GAAACAAGTT GTTCCCTGAA
TCAGTCATCA GAAATATTAT GTATCAAATA TTGCAAGGGC TGGCTTTTAT CCATAAACAT
GGCTTTTTTC ATAGGGACAT GAAACCAGAA AACTTGCTTT GTATGGGTCC AGAGCTTGTG
AAAATTGCTG ATTTTGGACT TGCAAGAGAA TTAAGGTCAC AGCCACCATA CACTGATTAT
GTATCTACCA GATGGTATCG TGCCCCTGAA GTTTTACTGA GATCTTCAGT TTATAGTTCT
CCCATTGATG TGTGGGCTGT TGGAAGTATC ATGGCTGAAC TCTATATGTT AAGGCCACTT
TTCCCAGGGA CAAGTGAGGT CGATGAAATC TTTAAAATTT GCCAAGTTTT AGGGACTCCC
AAAAAAAGTG ACTGGCCAGA AGGATACCAG CTGGCATCCT CTATGAACTT CCGTTTTCCC
CAGTGTGTTC CTATAAACTT AAAAACTCTT ATTCCCAATG CCAGTAATGA AGCTATTCAG
CTCATGACCG AAATGTTGAA TTGGGATCCA AAGAAACGAC CGACAGCAAG CCAGGCATTG
AAACACCCAT ATTTTCAAGT TGGTCAGGTA TTAGGCCCTT CGTCAAATCA TCTGGAATCA
AAACAGTCTT TAAATAAGCA GCTGCAACCA TTAGAATCAA AGCCATCTTT AGTTGAGGTA
GAGCCTAAGC CTCTGCCGGA TATAATCGAT CAGGTTGTTG GACAACCCCA GCCAAAAACT
AGCCAGCAGC CACTGCAGCC CATTCAGCCG CCACAGAACC TGAGCGTCCA GCAACCTCCA
AAGCAACAGA GTCAGGAGAA ACCGCCACAA ACGCTATTCC CGAGCATCGT CAAAAACATG
CCAACTAAGC CAAATGGCAC ACTGAGTCAT AAAAGTGGTA GGAGGCGTTG GGGTCAGACT
ATCTTCAAGT CTGGAGATAG CTGGGAAGAG TTGGAGGACT ATGATTTCGG AGCCTCCCAT
TCCAAGAAGC CAAGCATGGG TGTTTTTAAA GAAAAAAGGA AAAAAGATTC TCCATTTCGG
CTTCCAGAGC CAGTACCCTC AGGCTCCAAC CACTCGACAG GGGAAAACAA GAGCTTACCT
GCTGTTACTT CCCTAAAATC TGATTCCGAA TTGTCAACTG CTCCAACCTC TAAACAGTAC
TACTTGAAAC AATCAAGATA TCTTCCAGGT GTGAATCCCA AGAAGGTGTC CTTGATAGCC
AGTGGAAAGG AAATAAACCC CCACACTTGG AGCAACCAGT TATTCCCCAA GTCACTGGGA
CCCGTTGGGG CAGAACTTGC TTTCAAAAGG AGCAATGCAG AAGAAAGCAT AATTAAACCA
ATCGAAAAAC TATCATGCAA TGAAACTTTT CCTGAAAAAT TAGAGGACCC ACAAGGAAAT
CTTGGAAGTT ATGCTACTTA CAATCAGTCA GGATATATTC CTTCCTTTCT CAAAAAAGAA
GTGCAGTCAG CTGGCCAGAG GATCCACTTA GCACCTCTCA ATGCAACGGC TTCAGAATAT
ACCTGGAACA CAAAAACTGG TCGGGGGCAG TTTTCAGGAC GTACTTATAA TCCTACAGCA
AAAAACCTAA ATATTGTGAA CCGTGCACAG CCCATTCCCT CAGTGCATGG GAGGACAGAC
TGGGTGGCCA AGTATGGAGG CCACCGGTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011512923.1
CDS49..1938
Misc Feature(1)91..843(+)
Misc Feature(2)97..843(+)
Misc Feature(3)136..597(+)
Misc Feature(4)136..597(+)
Misc Feature(5)178..420(+)
Misc Feature(6)415..483(+)
Translation
Position Chain Variation Link
10 10 a, g dbSNP:568939864
17 17 g, t dbSNP:550465691
44 44 a, g dbSNP:187458515
61 61 a, g dbSNP:568250759
68 68 c, g dbSNP:72821595
76 76 a, c dbSNP:528466092
83 83 a, c dbSNP:552261918
86 86 a, g dbSNP:776564712
89 89 a, c dbSNP:777296559
100 100 a, g dbSNP:755003228
110 110 a, g dbSNP:560065834
116 116 g, t dbSNP:749261144
129 129 a, g dbSNP:779850347
132 132 c, g dbSNP:755994791
161 161 a, t dbSNP:568872972
163 163 a, g dbSNP:758042729
165 165 g, t dbSNP:371263215
175 175 a, g dbSNP:764738214
176 176 c, t dbSNP:761118698
184 184 c, t dbSNP:750895183
193 193 c, g dbSNP:767920227
206 206 a, g dbSNP:762158772
209 209 a, g dbSNP:774612770
213 213 c, t dbSNP:150892331
220 220 -, tttat dbSNP:781376741
223 223 a, g dbSNP:763168003
248 248 g, t dbSNP:775458619
249 249 -, ct dbSNP:757392221
259 259 a, c dbSNP:79404971
262 262 a, g dbSNP:769859859
276 276 a, g dbSNP:759659345
284 284 c, t dbSNP:776770214
286 286 c, g dbSNP:771009179
291 291 a, g dbSNP:746881510
301 301 a, g dbSNP:367716586
306 306 -, tat dbSNP:777554686
308 308 a, t dbSNP:773172706
309 309 a, g dbSNP:771852650
331 331 -, g dbSNP:527236082
347 347 a, g dbSNP:200506656
360 360 c, t dbSNP:200916721
362 362 a, g dbSNP:762987092
366 366 c, t dbSNP:773263219
379 379 a, c dbSNP:387906646
384 384 a, g dbSNP:771805362
385 385 -, cttc dbSNP:760171531
387 387 a, t dbSNP:747959498
391 391 a, g dbSNP:773956623
393 393 -, atg dbSNP:772837652
395 395 g, t dbSNP:768356424
396 396 g, t dbSNP:373994062
398 398 c, t dbSNP:779562217
402 402 a, g, t dbSNP:201804752
406 406 a, g dbSNP:747553199
412 412 a, g dbSNP:141420368
413 413 c, t dbSNP:758750076
426 426 a, g dbSNP:753121326
428 428 a, t dbSNP:765454168
430 430 a, g dbSNP:112561079
431 431 c, g dbSNP:755260124
441 441 a, g dbSNP:754068927
446 446 c, g dbSNP:766619005
455 455 c, g dbSNP:760809915
460 460 a, t dbSNP:375659310
467 467 a, g dbSNP:138795058
472 472 c, t dbSNP:372605229
475 475 a, t dbSNP:145065729
476 476 c, t dbSNP:774229391
479 479 a, g dbSNP:768283205
487 487 c, t dbSNP:527236081
488 488 a, g dbSNP:387906648
506 506 a, t dbSNP:373520122
509 509 c, g dbSNP:369612015
510 510 a, g dbSNP:748742307
511 511 a, t dbSNP:779260712
521 521 a, c dbSNP:769213821
522 522 c, t dbSNP:749659651
523 523 a, g dbSNP:572867029
527 527 c, t dbSNP:558155479
532 532 a, g dbSNP:747216229
533 533 c, t dbSNP:750559316
538 538 a, g dbSNP:781377439
540 540 a, g dbSNP:376610958
544 544 a, g dbSNP:527236080
547 547 g, t dbSNP:141312468
551 551 -, g dbSNP:770308128
556 556 a, g dbSNP:56215624
572 572 a, g dbSNP:752496358
574 574 -, at dbSNP:746288738
590 590 c, t dbSNP:369797377
597 597 a, g dbSNP:759210520
606 606 a, g dbSNP:138302657
609 609 c, t dbSNP:765957619
613 613 a, g dbSNP:760103192
630 630 a, c dbSNP:143572116
637 637 a, t dbSNP:769287766
638 638 c, t dbSNP:182178532
665 665 c, g dbSNP:112257972
666 666 a, g dbSNP:770348792
669 669 a, c dbSNP:375351078
677 677 a, t dbSNP:530738750
680 680 g, t dbSNP:771061280
681 681 a, g dbSNP:747215914
691 691 a, g dbSNP:754415973
700 700 c, t dbSNP:111618569
701 701 a, g dbSNP:746596336
709 709 c, t dbSNP:779514800
710 710 a, g dbSNP:201114107
746 746 a, g dbSNP:371639894
747 747 a, t dbSNP:377107543
750 750 c, t dbSNP:778820480
758 758 a, g dbSNP:754864509
769 769 a, c, g dbSNP:368102323
777 777 c, t dbSNP:199949446
778 778 a, g dbSNP:199689074
789 789 c, t dbSNP:767172193
796 796 c, t dbSNP:761403711
805 805 c, t dbSNP:753314164
806 806 a, g dbSNP:765675052
809 809 c, t dbSNP:149734152
814 814 a, g dbSNP:776892781
826 826 c, t dbSNP:756912445
834 834 c, t dbSNP:55950618
842 842 g, t dbSNP:763618857
843 843 g, t dbSNP:759984800
853 853 c, g dbSNP:754349931
855 855 a, g dbSNP:766693820
858 858 a, g dbSNP:147570352
862 862 a, g dbSNP:200901108
869 869 c, t dbSNP:773485283
870 870 a, g dbSNP:772299314
872 872 c, g dbSNP:761959677
893 893 a, t dbSNP:774449472
901 901 a, g dbSNP:199921765
902 902 a, t dbSNP:768757013
910 910 c, g dbSNP:186218889
911 911 g, t dbSNP:775490393
920 920 c, t dbSNP:769534346
932 932 c, g dbSNP:745670820
938 938 -, t dbSNP:777909571
939 939 a, c dbSNP:781065923
940 940 a, g dbSNP:757000356
951 951 c, g dbSNP:746612091
958 958 a, c dbSNP:762412793
959 959 c, g, t dbSNP:200641218
965 965 c, t dbSNP:371971492
966 966 a, g dbSNP:145014649
967 967 g, t dbSNP:367903138
972 972 a, c dbSNP:750794210
975 975 c, t dbSNP:767830843
976 976 a, g dbSNP:201812469
978 978 g, t dbSNP:17579447
979 979 c, t dbSNP:774598986
985 985 g, t dbSNP:764142857
993 993 -, acccca dbSNP:758224960
996 996 c, t dbSNP:763072144
998 998 -, a dbSNP:748139115
1002 1002 a, c dbSNP:775374397
1015 1015 c, g dbSNP:769859243
1026 1026 a, g dbSNP:139604404
1029 1029 c, g dbSNP:776588742
1031 1031 c, t dbSNP:770829149
1035 1035 c, g dbSNP:746701788
1037 1037 c, t dbSNP:371774117
1038 1038 a, g dbSNP:757905846
1040 1040 c, t dbSNP:747711453
1042 1042 c, t dbSNP:778223936
1044 1044 c, g dbSNP:756648303
1047 1047 c, g dbSNP:750884327
1051 1051 a, g dbSNP:767905978
1053 1053 c, t dbSNP:199694073
1054 1054 a, g dbSNP:751783623
1060 1060 c, t dbSNP:200300453
1062 1062 a, g dbSNP:143424442
1075 1075 -, ca dbSNP:755434575
1075 1075 c, g dbSNP:763018137
1084 1084 a, g dbSNP:201558986
1086 1086 a, g dbSNP:775668388
1089 1089 a, c dbSNP:765357899
1091 1091 -, cc dbSNP:141174473
1091 1091 c, g, t dbSNP:776680313
1092 1092 a, g dbSNP:770917055
1100 1100 c, t dbSNP:201494324
1101 1101 c, g dbSNP:772828352
1109 1109 c, t dbSNP:771764863
1112 1112 a, g dbSNP:747716951
1117 1117 a, g dbSNP:778510807
1119 1119 c, t dbSNP:768120333
1128 1128 c, g dbSNP:150744360
1130 1130 c, t dbSNP:781652966
1137 1137 a, g dbSNP:753029772
1142 1142 a, g dbSNP:55773478
1154 1154 c, g dbSNP:755195964
1167 1167 -, t dbSNP:753698504
1174 1174 c, t dbSNP:753974545
1175 1175 a, g, t dbSNP:62000445
1179 1179 c, g dbSNP:750310318
1182 1182 c, t dbSNP:551658772
1188 1188 a, t dbSNP:767409599
1196 1196 a, c dbSNP:200533678
1206 1206 c, t dbSNP:148549681
1211 1211 a, g dbSNP:763737966
1219 1219 c, t dbSNP:762551107
1220 1220 c, t dbSNP:144848825
1229 1229 a, g dbSNP:200944697
1236 1236 c, t dbSNP:115185609
1237 1237 a, g dbSNP:773588644
1238 1238 a, g dbSNP:549436795
1240 1240 g, t dbSNP:748539209
1241 1241 c, t dbSNP:779355214
1253 1253 a, g dbSNP:755285965
1257 1257 g, t dbSNP:137918175
1261 1261 a, c dbSNP:780233328
1268 1268 a, g dbSNP:139003494
1269 1269 c, t dbSNP:750428776
1279 1279 a, g dbSNP:767365219
1282 1282 a, t dbSNP:757149659
1287 1287 a, g dbSNP:751385674
1294 1294 g, t dbSNP:201502742
1296 1296 g, t dbSNP:760421859
1302 1302 a, g dbSNP:775091188
1306 1306 c, t dbSNP:764870230
1307 1307 a, g dbSNP:758956117
1308 1308 c, g dbSNP:762462087
1321 1321 c, g dbSNP:556048925
1322 1322 c, t dbSNP:769213984
1329 1329 a, c dbSNP:763420270
1332 1332 c, t dbSNP:775852553
1333 1333 c, t dbSNP:769938480
1338 1338 a, c dbSNP:746051642
1343 1343 c, t dbSNP:200057744
1345 1345 a, g dbSNP:193132857
1346 1346 a, c dbSNP:547372600
1347 1347 -, ag dbSNP:762837543
1350 1350 a, g dbSNP:747015059
1351 1351 -, g dbSNP:777144918
1357 1357 a, g dbSNP:777674662
1372 1372 a, g dbSNP:376120162
1373 1373 c, t dbSNP:146071625
1378 1378 a, g, t dbSNP:754612195
1395 1395 c, t dbSNP:184920692
1396 1396 a, g dbSNP:149006687
1403 1403 -, tgtc dbSNP:771209345
1405 1405 a, g dbSNP:760262193
1408 1408 a, g dbSNP:369794041
1410 1410 a, t dbSNP:749890675
1425 1425 a, g dbSNP:764699614
1427 1427 a, g dbSNP:763510409
1440 1440 a, g dbSNP:775738175
1444 1444 a, g dbSNP:770170708
1445 1445 g, t dbSNP:759716806
1451 1451 c, t dbSNP:776906266
1455 1455 a, g dbSNP:779438081
1459 1459 a, g dbSNP:776804120
1471 1471 -, aag dbSNP:375070639
1471 1471 a, g dbSNP:766593207
1472 1472 a, c, t dbSNP:773304876
1474 1474 g, t dbSNP:772119574
1476 1476 a, g dbSNP:748079104
1490 1490 -, g dbSNP:774717726
1508 1508 c, t dbSNP:752021255
1510 1510 -, c dbSNP:748216114
1512 1512 a, c, g dbSNP:375261960
1516 1516 c, t dbSNP:779951600
1521 1521 c, t dbSNP:755850716
1528 1528 g, t dbSNP:745490858
1549 1549 c, t dbSNP:567083
1551 1551 c, t dbSNP:756785609
1553 1553 c, t dbSNP:192591726
1559 1559 c, t dbSNP:765787091
1583 1583 a, c dbSNP:755373628
1584 1584 c, t dbSNP:754233012
1609 1609 a, g dbSNP:374286792
1611 1611 a, c dbSNP:745580809
1612 1612 a, g dbSNP:769238012
1628 1628 a, g dbSNP:371455796
1635 1635 c, t dbSNP:747805700
1662 1662 a, g dbSNP:552111002
1687 1687 c, t dbSNP:761680845
1689 1689 a, c dbSNP:186643840
1691 1691 a, g dbSNP:761897291
1704 1704 c, t dbSNP:751678907
1706 1706 c, t dbSNP:79544660
1708 1708 c, t dbSNP:371838435
1713 1713 c, t dbSNP:775561335
1714 1714 c, t dbSNP:56217305
1723 1723 a, c dbSNP:759417469
1724 1724 a, g dbSNP:776221542
1731 1731 a, g dbSNP:770648753
1732 1732 a, c dbSNP:746594889
1734 1734 c, g dbSNP:556910057
1746 1746 a, g dbSNP:111281539
1753 1753 c, t dbSNP:200319110
1766 1766 c, t dbSNP:771453698
1769 1769 a, g dbSNP:200866765
1776 1776 a, g dbSNP:143121053
1778 1778 c, t dbSNP:756558713
1779 1779 c, t dbSNP:142308835
1794 1794 a, g dbSNP:770320899
1803 1803 a, t dbSNP:757841911
1806 1806 a, c, t dbSNP:570463236
1809 1809 -, tcgg dbSNP:760121453
1810 1810 c, t dbSNP:199594233
1811 1811 a, g dbSNP:368535018
1825 1825 c, g dbSNP:201628941
1828 1828 c, t dbSNP:553591095
1829 1829 a, g dbSNP:758636733
1832 1832 c, t dbSNP:752846705
1838 1838 a, c dbSNP:371429892
1840 1840 c, g dbSNP:147251038
1841 1841 c, g dbSNP:753694997
1842 1842 c, t dbSNP:766252727
1846 1846 a, g dbSNP:760381134
1853 1853 a, c dbSNP:180826550
1854 1854 a, c dbSNP:767167233
1862 1862 g, t dbSNP:761401422
1866 1866 a, g dbSNP:773894456
1870 1870 c, t dbSNP:768134966
1871 1871 a, g, t dbSNP:777002916
1879 1879 c, g, t dbSNP:747349238
1886 1886 c, t dbSNP:778235109
1890 1890 -, ag dbSNP:754210226
1896 1896 c, t dbSNP:126405
1897 1897 a, g dbSNP:748349220
1899 1899 a, g dbSNP:537862624
1901 1901 a, g dbSNP:377372773
1912 1912 a, g dbSNP:567613995
1913 1913 g, t dbSNP:766229047
1933 1933 c, t dbSNP:375794836
1934 1934 a, c, g dbSNP:767273634
1945 1945 a, g dbSNP:370549111
1949 1949 g, t dbSNP:773982305
1950 1950 a, g dbSNP:377201252
1958 1958 -, ctac dbSNP:766869339
1971 1971 c, t dbSNP:372487961
1972 1972 c, t dbSNP:78074137
1973 1973 a, g dbSNP:376336149
1974 1974 a, t dbSNP:747511670
1975 1975 a, c dbSNP:773751765
1981 1981 c, t dbSNP:373416620
1988 1988 c, g dbSNP:369955868
1989 1989 c, t dbSNP:560376669
2038 2038 a, g dbSNP:373312456
2058 2058 c, g dbSNP:536214552
2072 2072 c, g dbSNP:552039665
2110 2110 c, g dbSNP:753480982
2126 2126 c, t dbSNP:3734395
2164 2164 a, g dbSNP:547465110
2216 2216 a, t dbSNP:544885539
2217 2217 a, c dbSNP:575950279
2221 2221 c, g dbSNP:200154458
2222 2222 c, t dbSNP:542247606
2230 2230 a, g dbSNP:753241358
2250 2250 -, a dbSNP:769006848
2269 2269 -, ta dbSNP:558307147
2315 2315 c, t dbSNP:572139433
2368 2368 c, t dbSNP:763523138
2402 2402 c, g dbSNP:553427676
2428 2428 c, t dbSNP:538465920
2524 2524 -, t dbSNP:397705840
2539 2539 -, t, tt dbSNP:60516370
2539 2539 g, t dbSNP:75280442
2540 2540 g, t dbSNP:76389200
2541 2541 a, g dbSNP:376366712
2545 2545 a, g dbSNP:577379885
2559 2559 c, g dbSNP:527434856

Target ORF information:

RefSeq Version XM_011514621
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens male germ cell-associated kinase (MAK), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011514621

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGAAACACA CAAGACGCCG GAGCTTCACA AGGGAGCGAA CGAGGATGAA GAGAAAGTTC 
TATTCTTGGG ATGAATGCAT GAACTTGAGA GAAGTTAAGT CTCTGAAGAA ACTTAATCAT
GCCAATGTTA TTAAATTGAA AGAAGTTATC AGAGAAAATG ACCATCTTTA TTTTATATTT
GAATATATGA AAGAAAACCT CTATCAATTA ATGAAAGACA GAAACAAGTT GTTCCCTGAA
TCAGTCATCA GAAATATTAT GTATCAAATA TTGCAAGGGC TGGCTTTTAT CCATAAACAT
GGCTTTTTTC ATAGGGACAT GAAACCAGAA AACTTGCTTT GTATGGGTCC AGAGCTTGTG
AAAATTGCTG ATTTTGGACT TGCAAGAGAA TTAAGGTCAC AGCCACCATA CACTGATTAT
GTATCTACCA GATGGTATCG TGCCCCTGAA GTTTTACTGA GATCTTCAGT TTATAGTTCT
CCCATTGATG TGTGGGCTGT TGGAAGTATC ATGGCTGAAC TCTATATGTT AAGGCCACTT
TTCCCAGGGA CAAGTGAGGT CGATGAAATC TTTAAAATTT GCCAAGTTTT AGGGACTCCC
AAAAAAAGTG ACTGGCCAGA AGGATACCAG CTGGCATCCT CTATGAACTT CCGTTTTCCC
CAGTGTGTTC CTATAAACTT AAAAACTCTT ATTCCCAATG CCAGTAATGA AGCTATTCAG
CTCATGACCG AAATGTTGAA TTGGGATCCA AAGAAACGAC CGACAGCAAG CCAGGCATTG
AAACACCCAT ATTTTCAAGT TGGTCAGGTA TTAGGCCCTT CGTCAAATCA TCTGGAATCA
AAACAGTCTT TAAATAAGCA GCTGCAACCA TTAGAATCAA AGCCATCTTT AGTTGAGGTA
GAGCCTAAGC CTCTGCCGGA TATAATCGAT CAGGTTGTTG GACAACCCCA GCCAAAAACT
AGCCAGCAGC CACTGCAGCC CATTCAGCCG CCACAGAACC TGAGCGTCCA GCAACCTCCA
AAGCAACAGA GTCAGGAGAA ACCGCCACAA ACGCTATTCC CGAGCATCGT CAAAAACATG
CCAACTAAGC CAAATGGCAC ACTGAGTCAT AAAAGTGGTA GGAGGCGTTG GGGTCAGACT
ATCTTCAAGT CTGGAGATAG CTGGGAAGAG TTGGAGGACT ATGATTTCGG AGCCTCCCAT
TCCAAGAAGC CAAGCATGGG TGTTTTTAAA GAAAAAAGGA AAAAAGATTC TCCATTTCGG
CTTCCAGAGC CAGTACCCTC AGGCTCCAAC CACTCGACAG GGGAAAACAA GAGCTTACCT
GCTGTTACTT CCCTAAAATC TGATTCCGAA TTGTCAACTG CTCCAACCTC TAAACAGTAC
TACTTGAAAC AATCAAGATA TCTTCCAGGT GTGAATCCCA AGAAGGTGTC CTTGATAGCC
AGTGGAAAGG AAATAAACCC CCACACTTGG AGCAACCAGT TATTCCCCAA GTCACTGGGA
CCCGTTGGGG CAGAACTTGC TTTCAAAAGG AGCAATGCAG AAGAAAGCAT AATTAAACCA
ATCGAAAAAC TATCATGCAA TGAAACTTTT CCTGAAAAAT TAGAGGACCC ACAAGGAAAT
CTTGGAAGTT ATGCTACTTA CAATCAGTCA GGATATATTC CTTCCTTTCT CAAAAAAGAA
GTGCAGTCAG CTGGCCAGAG GATCCACTTA GCACCTCTCA ATGCAACGGC TTCAGAATAT
ACCTGGAACA CAAAAACTGG TCGGGGGCAG TTTTCAGGAC GTACTTATAA TCCTACAGCA
AAAAACCTAA ATATTGTGAA CCGTGCACAG CCCATTCCCT CAGTGCATGG GAGGACAGAC
TGGGTGGCCA AGTATGGAGG CCACCGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20689
Accession Version XM_011514622.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1752bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase MAK isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007592.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGAACCGAT ACACAACCAT GAGACAGTTG GGGGACGGCA CGTATGGGAG TGTGCTTATG 
GGCAAGAGTA ATGAATCCGG GGAGCTGGTG GCCATCAAAA GGATGAAGAG AAAGTTCTAT
TCTTGGGATG AATGCATGAA CTTGAGAGAA GTTAAGTCTC TGAAGAAACT TAATCATGCC
AATGTTATTA AATTGAAAGA AGTTATCAGA GAAAATGACC ATCTTTATTT TATATTTGAA
TATATGAAAG AAAACCTCTA TCAATTAATG AAAGACAGAA ACAAGTTGTT CCCTGAATCA
GTCATCAGAA ATATTATGTA TCAAATATTG CAAGGGCTGG CTTTTATCCA TAAACATGGC
TTTTTTCATA GGGACATGAA ACCAGAAAAC TTGCTTTGTA TGGGTCCAGA GCTTGTGAAA
ATTGCTGATT TTGGACTTGC AAGAGAATTA AGGTCACAGC CACCATACAC TGATTATGTA
TCTACCAGAT GGTATCGTGC CCCTGAAGTT TTACTGAGAT CTTCAGTTTA TAGTTCTCCC
ATTGATGTGT GGGCTGTTGG AAGTATCATG GCTGAACTCT ATATGTTAAG GCCACTTTTC
CCAGGGACAA GTGAGGTCGA TGAAATCTTT AAAATTTGCC AAGTTTTAGG GACTCCCAAA
AAAAGTGACT GGCCAGAAGG ATACCAGCTG GCATCCTCTA TGAACTTCCG TTTTCCCCAG
TGTGTTCCTA TAAACTTAAA AACTCTTATT CCCAATGCCA GTAATGAAGC TATTCAGCTC
ATGACCGAAA TGTTGAATTG GGATCCAAAG AAACGACCGA CAGCAAGCCA GGCATTGAAA
CACCCATATT TTCAAGTTGG TCAGGTATTA GGCCCTTCGT CAAATCATCT GGAATCAAAA
CAGTCTTTAA ATAAGCAGCT GCAACCATTA GAATCAAAGC CATCTTTAGT TGAGGTAGAG
CCTAAGCCTC TGCCGGATAT AATCGATCAG GTTGTTGGAC AACCCCAGCC AAAAACTAGC
CAGCAGCCAC TGCAGCCCAT TCAGCCGCCA CAGAACCTGA GCGTCCAGCA ACCTCCAAAG
CAACAGAGTC AGGAGAAACC GCCACAAACG CTATTCCCGA GCATCGTCAA AAACATGCCA
ACTAAGCCAA ATGGCACACT GAGTCATAAA AGTGGTAGGA GGCGTTGGGG TCAGACTATC
TTCAAGTCTG GAGATAGCTG GGAAGAGTTG GAGGACTATG ATTTCGGAGC CTCCCATTCC
AAGAAGCCAA GCATGGGTGT TTTTAAAGAA AAAAGGAAAA AAGATTCTCC ATTTCGGCTT
CCAGAGCCAG TACCCTCAGG CTCCAACCAC TCGACAGGGG AAAACAAGAG CTTACCTGCT
GTTACTTCCC TAAAATCTGA TTCCGAATTG TCAACTGCTC CAACCTCTAA ACAGTACTAC
TTGAAACAAT CAAGATATCT TCCAGGTGTG AATCCCAAGA AGGTGTCCTT GATAGCCAGT
GGAAAGGAAA TAAACCCCCA CACTTGGAGC AACCAGTTAT TCCCCAAGTC ACTGGGACCC
GTTGGGGCAG AACTTGCTTT CAAAAGGAGC AATGCAGAAT ATACCTGGAA CACAAAAACT
GGTCGGGGGC AGTTTTCAGG ACGTACTTAT AATCCTACAG CAAAAAACCT AAATATTGTG
AACCGTGCAC AGCCCATTCC CTCAGTGCAT GGGAGGACAG ACTGGGTGGC CAAGTATGGA
GGCCACCGGT AG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011512924.1
CDS333..2084
Misc Feature(1)342..1184(+)
Misc Feature(2)342..1184(+)
Misc Feature(3)360..938(+)
Misc Feature(4)360..761(+)
Misc Feature(5)477..938(+)
Misc Feature(6)756..824(+)
Translation
Position Chain Variation Link
2 2 c, t dbSNP:75712436
21 21 a, g dbSNP:568939864
28 28 g, t dbSNP:550465691
55 55 a, g dbSNP:187458515
72 72 a, g dbSNP:568250759
79 79 c, g dbSNP:72821595
87 87 a, c dbSNP:528466092
94 94 a, c dbSNP:552261918
97 97 a, g dbSNP:776564712
100 100 a, c dbSNP:777296559
157 157 c, t dbSNP:546568347
174 174 a, g dbSNP:528500811
183 183 g, t dbSNP:570847928
209 209 c, t dbSNP:552689175
267 267 a, g dbSNP:181775528
287 287 a, c dbSNP:368252042
291 291 a, g dbSNP:781639259
301 301 a, g dbSNP:757503362
304 304 a, g dbSNP:375783146
311 311 a, t dbSNP:370663263
339 339 a, c, t dbSNP:558628181
340 340 a, g dbSNP:752676297
344 344 c, t dbSNP:765158321
348 348 a, g dbSNP:200406214
351 351 a, g dbSNP:753647340
360 360 c, t dbSNP:202067704
364 364 a, g dbSNP:760408768
368 368 c, t dbSNP:138352622
369 369 a, c, g dbSNP:387906647
373 373 c, t dbSNP:776197156
374 374 a, g dbSNP:770432926
378 378 a, g dbSNP:751370670
382 382 a, g dbSNP:777163256
383 383 c, t dbSNP:771149172
386 386 a, g dbSNP:747307777
389 389 c, t dbSNP:777830548
410 410 a, c, t dbSNP:542075413
411 411 a, c, g dbSNP:754916169
414 414 -, c dbSNP:35709746
422 422 a, g dbSNP:753737265
425 425 -, c dbSNP:753804603
441 441 a, g dbSNP:755003228
451 451 a, g dbSNP:560065834
457 457 g, t dbSNP:749261144
470 470 a, g dbSNP:779850347
473 473 c, g dbSNP:755994791
502 502 a, t dbSNP:568872972
504 504 a, g dbSNP:758042729
506 506 g, t dbSNP:371263215
516 516 a, g dbSNP:764738214
517 517 c, t dbSNP:761118698
525 525 c, t dbSNP:750895183
534 534 c, g dbSNP:767920227
547 547 a, g dbSNP:762158772
550 550 a, g dbSNP:774612770
554 554 c, t dbSNP:150892331
561 561 -, tttat dbSNP:781376741
564 564 a, g dbSNP:763168003
589 589 g, t dbSNP:775458619
590 590 -, ct dbSNP:757392221
600 600 a, c dbSNP:79404971
603 603 a, g dbSNP:769859859
617 617 a, g dbSNP:759659345
625 625 c, t dbSNP:776770214
627 627 c, g dbSNP:771009179
632 632 a, g dbSNP:746881510
642 642 a, g dbSNP:367716586
647 647 -, tat dbSNP:777554686
649 649 a, t dbSNP:773172706
650 650 a, g dbSNP:771852650
672 672 -, g dbSNP:527236082
688 688 a, g dbSNP:200506656
701 701 c, t dbSNP:200916721
703 703 a, g dbSNP:762987092
707 707 c, t dbSNP:773263219
720 720 a, c dbSNP:387906646
725 725 a, g dbSNP:771805362
726 726 -, cttc dbSNP:760171531
728 728 a, t dbSNP:747959498
732 732 a, g dbSNP:773956623
734 734 -, atg dbSNP:772837652
736 736 g, t dbSNP:768356424
737 737 g, t dbSNP:373994062
739 739 c, t dbSNP:779562217
743 743 a, g, t dbSNP:201804752
747 747 a, g dbSNP:747553199
753 753 a, g dbSNP:141420368
754 754 c, t dbSNP:758750076
767 767 a, g dbSNP:753121326
769 769 a, t dbSNP:765454168
771 771 a, g dbSNP:112561079
772 772 c, g dbSNP:755260124
782 782 a, g dbSNP:754068927
787 787 c, g dbSNP:766619005
796 796 c, g dbSNP:760809915
801 801 a, t dbSNP:375659310
808 808 a, g dbSNP:138795058
813 813 c, t dbSNP:372605229
816 816 a, t dbSNP:145065729
817 817 c, t dbSNP:774229391
820 820 a, g dbSNP:768283205
828 828 c, t dbSNP:527236081
829 829 a, g dbSNP:387906648
847 847 a, t dbSNP:373520122
850 850 c, g dbSNP:369612015
851 851 a, g dbSNP:748742307
852 852 a, t dbSNP:779260712
862 862 a, c dbSNP:769213821
863 863 c, t dbSNP:749659651
864 864 a, g dbSNP:572867029
868 868 c, t dbSNP:558155479
873 873 a, g dbSNP:747216229
874 874 c, t dbSNP:750559316
879 879 a, g dbSNP:781377439
881 881 a, g dbSNP:376610958
885 885 a, g dbSNP:527236080
888 888 g, t dbSNP:141312468
892 892 -, g dbSNP:770308128
897 897 a, g dbSNP:56215624
913 913 a, g dbSNP:752496358
915 915 -, at dbSNP:746288738
931 931 c, t dbSNP:369797377
938 938 a, g dbSNP:759210520
947 947 a, g dbSNP:138302657
950 950 c, t dbSNP:765957619
954 954 a, g dbSNP:760103192
971 971 a, c dbSNP:143572116
978 978 a, t dbSNP:769287766
979 979 c, t dbSNP:182178532
1006 1006 c, g dbSNP:112257972
1007 1007 a, g dbSNP:770348792
1010 1010 a, c dbSNP:375351078
1018 1018 a, t dbSNP:530738750
1021 1021 g, t dbSNP:771061280
1022 1022 a, g dbSNP:747215914
1032 1032 a, g dbSNP:754415973
1041 1041 c, t dbSNP:111618569
1042 1042 a, g dbSNP:746596336
1050 1050 c, t dbSNP:779514800
1051 1051 a, g dbSNP:201114107
1087 1087 a, g dbSNP:371639894
1088 1088 a, t dbSNP:377107543
1091 1091 c, t dbSNP:778820480
1099 1099 a, g dbSNP:754864509
1110 1110 a, c, g dbSNP:368102323
1118 1118 c, t dbSNP:199949446
1119 1119 a, g dbSNP:199689074
1130 1130 c, t dbSNP:767172193
1137 1137 c, t dbSNP:761403711
1146 1146 c, t dbSNP:753314164
1147 1147 a, g dbSNP:765675052
1150 1150 c, t dbSNP:149734152
1155 1155 a, g dbSNP:776892781
1167 1167 c, t dbSNP:756912445
1175 1175 c, t dbSNP:55950618
1183 1183 g, t dbSNP:763618857
1184 1184 g, t dbSNP:759984800
1194 1194 c, g dbSNP:754349931
1196 1196 a, g dbSNP:766693820
1199 1199 a, g dbSNP:147570352
1203 1203 a, g dbSNP:200901108
1210 1210 c, t dbSNP:773485283
1211 1211 a, g dbSNP:772299314
1213 1213 c, g dbSNP:761959677
1234 1234 a, t dbSNP:774449472
1242 1242 a, g dbSNP:199921765
1243 1243 a, t dbSNP:768757013
1251 1251 c, g dbSNP:186218889
1252 1252 g, t dbSNP:775490393
1261 1261 c, t dbSNP:769534346
1273 1273 c, g dbSNP:745670820
1279 1279 -, t dbSNP:777909571
1280 1280 a, c dbSNP:781065923
1281 1281 a, g dbSNP:757000356
1292 1292 c, g dbSNP:746612091
1299 1299 a, c dbSNP:762412793
1300 1300 c, g, t dbSNP:200641218
1306 1306 c, t dbSNP:371971492
1307 1307 a, g dbSNP:145014649
1308 1308 g, t dbSNP:367903138
1313 1313 a, c dbSNP:750794210
1316 1316 c, t dbSNP:767830843
1317 1317 a, g dbSNP:201812469
1319 1319 g, t dbSNP:17579447
1320 1320 c, t dbSNP:774598986
1326 1326 g, t dbSNP:764142857
1334 1334 -, acccca dbSNP:758224960
1337 1337 c, t dbSNP:763072144
1339 1339 -, a dbSNP:748139115
1343 1343 a, c dbSNP:775374397
1356 1356 c, g dbSNP:769859243
1367 1367 a, g dbSNP:139604404
1370 1370 c, g dbSNP:776588742
1372 1372 c, t dbSNP:770829149
1376 1376 c, g dbSNP:746701788
1378 1378 c, t dbSNP:371774117
1379 1379 a, g dbSNP:757905846
1381 1381 c, t dbSNP:747711453
1383 1383 c, t dbSNP:778223936
1385 1385 c, g dbSNP:756648303
1388 1388 c, g dbSNP:750884327
1392 1392 a, g dbSNP:767905978
1394 1394 c, t dbSNP:199694073
1395 1395 a, g dbSNP:751783623
1401 1401 c, t dbSNP:200300453
1403 1403 a, g dbSNP:143424442
1416 1416 -, ca dbSNP:755434575
1416 1416 c, g dbSNP:763018137
1425 1425 a, g dbSNP:201558986
1427 1427 a, g dbSNP:775668388
1430 1430 a, c dbSNP:765357899
1432 1432 -, cc dbSNP:141174473
1432 1432 c, g, t dbSNP:776680313
1433 1433 a, g dbSNP:770917055
1441 1441 c, t dbSNP:201494324
1442 1442 c, g dbSNP:772828352
1450 1450 c, t dbSNP:771764863
1453 1453 a, g dbSNP:747716951
1458 1458 a, g dbSNP:778510807
1460 1460 c, t dbSNP:768120333
1469 1469 c, g dbSNP:150744360
1471 1471 c, t dbSNP:781652966
1478 1478 a, g dbSNP:753029772
1483 1483 a, g dbSNP:55773478
1495 1495 c, g dbSNP:755195964
1508 1508 -, t dbSNP:753698504
1515 1515 c, t dbSNP:753974545
1516 1516 a, g, t dbSNP:62000445
1520 1520 c, g dbSNP:750310318
1523 1523 c, t dbSNP:551658772
1529 1529 a, t dbSNP:767409599
1537 1537 a, c dbSNP:200533678
1547 1547 c, t dbSNP:148549681
1552 1552 a, g dbSNP:763737966
1560 1560 c, t dbSNP:762551107
1561 1561 c, t dbSNP:144848825
1570 1570 a, g dbSNP:200944697
1577 1577 c, t dbSNP:115185609
1578 1578 a, g dbSNP:773588644
1579 1579 a, g dbSNP:549436795
1581 1581 g, t dbSNP:748539209
1582 1582 c, t dbSNP:779355214
1594 1594 a, g dbSNP:755285965
1598 1598 g, t dbSNP:137918175
1602 1602 a, c dbSNP:780233328
1609 1609 a, g dbSNP:139003494
1610 1610 c, t dbSNP:750428776
1620 1620 a, g dbSNP:767365219
1623 1623 a, t dbSNP:757149659
1628 1628 a, g dbSNP:751385674
1635 1635 g, t dbSNP:201502742
1637 1637 g, t dbSNP:760421859
1643 1643 a, g dbSNP:775091188
1647 1647 c, t dbSNP:764870230
1648 1648 a, g dbSNP:758956117
1649 1649 c, g dbSNP:762462087
1662 1662 c, g dbSNP:556048925
1663 1663 c, t dbSNP:769213984
1670 1670 a, c dbSNP:763420270
1673 1673 c, t dbSNP:775852553
1674 1674 c, t dbSNP:769938480
1679 1679 a, c dbSNP:746051642
1684 1684 c, t dbSNP:200057744
1686 1686 a, g dbSNP:193132857
1687 1687 a, c dbSNP:547372600
1688 1688 -, ag dbSNP:762837543
1691 1691 a, g dbSNP:747015059
1692 1692 -, g dbSNP:777144918
1698 1698 a, g dbSNP:777674662
1713 1713 a, g dbSNP:376120162
1714 1714 c, t dbSNP:146071625
1719 1719 a, g, t dbSNP:754612195
1736 1736 c, t dbSNP:184920692
1737 1737 a, g dbSNP:149006687
1744 1744 -, tgtc dbSNP:771209345
1746 1746 a, g dbSNP:760262193
1749 1749 a, g dbSNP:369794041
1751 1751 a, t dbSNP:749890675
1766 1766 a, g dbSNP:764699614
1768 1768 a, g dbSNP:763510409
1781 1781 a, g dbSNP:775738175
1785 1785 a, g dbSNP:770170708
1786 1786 g, t dbSNP:759716806
1792 1792 c, t dbSNP:776906266
1796 1796 a, g dbSNP:779438081
1800 1800 a, g dbSNP:776804120
1812 1812 -, aag dbSNP:375070639
1812 1812 a, g dbSNP:766593207
1813 1813 a, c, t dbSNP:773304876
1815 1815 g, t dbSNP:772119574
1817 1817 a, g dbSNP:748079104
1831 1831 -, g dbSNP:774717726
1849 1849 c, t dbSNP:752021255
1851 1851 -, c dbSNP:748216114
1853 1853 a, c, g dbSNP:375261960
1857 1857 c, t dbSNP:779951600
1862 1862 c, t dbSNP:755850716
1869 1869 g, t dbSNP:745490858
1890 1890 c, t dbSNP:567083
1892 1892 c, t dbSNP:756785609
1894 1894 c, t dbSNP:192591726
1900 1900 c, t dbSNP:765787091
1924 1924 a, c dbSNP:755373628
1925 1925 c, t dbSNP:754233012
1940 1940 a, g dbSNP:770320899
1949 1949 a, t dbSNP:757841911
1952 1952 a, c, t dbSNP:570463236
1955 1955 -, tcgg dbSNP:760121453
1956 1956 c, t dbSNP:199594233
1957 1957 a, g dbSNP:368535018
1971 1971 c, g dbSNP:201628941
1974 1974 c, t dbSNP:553591095
1975 1975 a, g dbSNP:758636733
1978 1978 c, t dbSNP:752846705
1984 1984 a, c dbSNP:371429892
1986 1986 c, g dbSNP:147251038
1987 1987 c, g dbSNP:753694997
1988 1988 c, t dbSNP:766252727
1992 1992 a, g dbSNP:760381134
1999 1999 a, c dbSNP:180826550
2000 2000 a, c dbSNP:767167233
2008 2008 g, t dbSNP:761401422
2012 2012 a, g dbSNP:773894456
2016 2016 c, t dbSNP:768134966
2017 2017 a, g, t dbSNP:777002916
2025 2025 c, g, t dbSNP:747349238
2032 2032 c, t dbSNP:778235109
2036 2036 -, ag dbSNP:754210226
2042 2042 c, t dbSNP:126405
2043 2043 a, g dbSNP:748349220
2045 2045 a, g dbSNP:537862624
2047 2047 a, g dbSNP:377372773
2058 2058 a, g dbSNP:567613995
2059 2059 g, t dbSNP:766229047
2079 2079 c, t dbSNP:375794836
2080 2080 a, c, g dbSNP:767273634
2091 2091 a, g dbSNP:370549111
2095 2095 g, t dbSNP:773982305
2096 2096 a, g dbSNP:377201252
2104 2104 -, ctac dbSNP:766869339
2117 2117 c, t dbSNP:372487961
2118 2118 c, t dbSNP:78074137
2119 2119 a, g dbSNP:376336149
2120 2120 a, t dbSNP:747511670
2121 2121 a, c dbSNP:773751765
2127 2127 c, t dbSNP:373416620
2134 2134 c, g dbSNP:369955868
2135 2135 c, t dbSNP:560376669
2184 2184 a, g dbSNP:373312456
2204 2204 c, g dbSNP:536214552
2218 2218 c, g dbSNP:552039665
2256 2256 c, g dbSNP:753480982
2272 2272 c, t dbSNP:3734395
2310 2310 a, g dbSNP:547465110

Target ORF information:

RefSeq Version XM_011514622
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens male germ cell-associated kinase (MAK), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011514622

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGAACCGAT ACACAACCAT GAGACAGTTG GGGGACGGCA CGTATGGGAG TGTGCTTATG 
GGCAAGAGTA ATGAATCCGG GGAGCTGGTG GCCATCAAAA GGATGAAGAG AAAGTTCTAT
TCTTGGGATG AATGCATGAA CTTGAGAGAA GTTAAGTCTC TGAAGAAACT TAATCATGCC
AATGTTATTA AATTGAAAGA AGTTATCAGA GAAAATGACC ATCTTTATTT TATATTTGAA
TATATGAAAG AAAACCTCTA TCAATTAATG AAAGACAGAA ACAAGTTGTT CCCTGAATCA
GTCATCAGAA ATATTATGTA TCAAATATTG CAAGGGCTGG CTTTTATCCA TAAACATGGC
TTTTTTCATA GGGACATGAA ACCAGAAAAC TTGCTTTGTA TGGGTCCAGA GCTTGTGAAA
ATTGCTGATT TTGGACTTGC AAGAGAATTA AGGTCACAGC CACCATACAC TGATTATGTA
TCTACCAGAT GGTATCGTGC CCCTGAAGTT TTACTGAGAT CTTCAGTTTA TAGTTCTCCC
ATTGATGTGT GGGCTGTTGG AAGTATCATG GCTGAACTCT ATATGTTAAG GCCACTTTTC
CCAGGGACAA GTGAGGTCGA TGAAATCTTT AAAATTTGCC AAGTTTTAGG GACTCCCAAA
AAAAGTGACT GGCCAGAAGG ATACCAGCTG GCATCCTCTA TGAACTTCCG TTTTCCCCAG
TGTGTTCCTA TAAACTTAAA AACTCTTATT CCCAATGCCA GTAATGAAGC TATTCAGCTC
ATGACCGAAA TGTTGAATTG GGATCCAAAG AAACGACCGA CAGCAAGCCA GGCATTGAAA
CACCCATATT TTCAAGTTGG TCAGGTATTA GGCCCTTCGT CAAATCATCT GGAATCAAAA
CAGTCTTTAA ATAAGCAGCT GCAACCATTA GAATCAAAGC CATCTTTAGT TGAGGTAGAG
CCTAAGCCTC TGCCGGATAT AATCGATCAG GTTGTTGGAC AACCCCAGCC AAAAACTAGC
CAGCAGCCAC TGCAGCCCAT TCAGCCGCCA CAGAACCTGA GCGTCCAGCA ACCTCCAAAG
CAACAGAGTC AGGAGAAACC GCCACAAACG CTATTCCCGA GCATCGTCAA AAACATGCCA
ACTAAGCCAA ATGGCACACT GAGTCATAAA AGTGGTAGGA GGCGTTGGGG TCAGACTATC
TTCAAGTCTG GAGATAGCTG GGAAGAGTTG GAGGACTATG ATTTCGGAGC CTCCCATTCC
AAGAAGCCAA GCATGGGTGT TTTTAAAGAA AAAAGGAAAA AAGATTCTCC ATTTCGGCTT
CCAGAGCCAG TACCCTCAGG CTCCAACCAC TCGACAGGGG AAAACAAGAG CTTACCTGCT
GTTACTTCCC TAAAATCTGA TTCCGAATTG TCAACTGCTC CAACCTCTAA ACAGTACTAC
TTGAAACAAT CAAGATATCT TCCAGGTGTG AATCCCAAGA AGGTGTCCTT GATAGCCAGT
GGAAAGGAAA TAAACCCCCA CACTTGGAGC AACCAGTTAT TCCCCAAGTC ACTGGGACCC
GTTGGGGCAG AACTTGCTTT CAAAAGGAGC AATGCAGAAT ATACCTGGAA CACAAAAACT
GGTCGGGGGC AGTTTTCAGG ACGTACTTAT AATCCTACAG CAAAAAACCT AAATATTGTG
AACCGTGCAC AGCCCATTCC CTCAGTGCAT GGGAGGACAG ACTGGGTGGC CAAGTATGGA
GGCCACCGGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58833
Accession Version XM_011514623.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1380bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase MAK isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007592.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCTGAAC TCTATATGTT AAGGCCACTT TTCCCAGGGA CAAGTGAGGT CGATGAAATC 
TTTAAAATTT GCCAAGTTTT AGGGACTCCC AAAAAAAGTG ACTGGCCAGA AGGATACCAG
CTGGCATCCT CTATGAACTT CCGTTTTCCC CAGTGTGTTC CTATAAACTT AAAAACTCTT
ATTCCCAATG CCAGTAATGA AGCTATTCAG CTCATGACCG AAATGTTGAA TTGGGATCCA
AAGAAACGAC CGACAGCAAG CCAGGCATTG AAACACCCAT ATTTTCAAGT TGGTCAGGTA
TTAGGCCCTT CGTCAAATCA TCTGGAATCA AAACAGTCTT TAAATAAGCA GCTGCAACCA
TTAGAATCAA AGCCATCTTT AGTTGAGGTA GAGCCTAAGC CTCTGCCGGA TATAATCGAT
CAGGTTGTTG GACAACCCCA GCCAAAAACT AGCCAGCAGC CACTGCAGCC CATTCAGCCG
CCACAGAACC TGAGCGTCCA GCAACCTCCA AAGCAACAGA GTCAGGAGAA ACCGCCACAA
ACGCTATTCC CGAGCATCGT CAAAAACATG CCAACTAAGC CAAATGGCAC ACTGAGTCAT
AAAAGTGGTA GGAGGCGTTG GGGTCAGACT ATCTTCAAGT CTGGAGATAG CTGGGAAGAG
TTGGAGGACT ATGATTTCGG AGCCTCCCAT TCCAAGAAGC CAAGCATGGG TGTTTTTAAA
GAAAAAAGGA AAAAAGATTC TCCATTTCGG CTTCCAGAGC CAGTACCCTC AGGCTCCAAC
CACTCGACAG GGGAAAACAA GAGCTTACCT GCTGTTACTT CCCTAAAATC TGATTCCGAA
TTGTCAACTG CTCCAACCTC TAAACAGTAC TACTTGAAAC AATCAAGATA TCTTCCAGGT
GTGAATCCCA AGAAGGTGTC CTTGATAGCC AGTGGAAAGG AAATAAACCC CCACACTTGG
AGCAACCAGT TATTCCCCAA GTCACTGGGA CCCGTTGGGG CAGAACTTGC TTTCAAAAGG
AGCAATGCAG AAGAAAGCAT AATTAAACCA ATCGAAAAAC TATCATGCAA TGAAACTTTT
CCTGAAAAAT TAGAGGACCC ACAAGGAAAT CTTGGAAGTT ATGCTACTTA CAATCAGTCA
GGATATATTC CTTCCTTTCT CAAAAAAGAA GTGCAGTCAG CTGGCCAGAG GATCCACTTA
GCACCTCTCA ATGCAACGGC TTCAGAATAT ACCTGGAACA CAAAAACTGG TCGGGGGCAG
TTTTCAGGAC GTACTTATAA TCCTACAGCA AAAAACCTAA ATATTGTGAA CCGTGCACAG
CCCATTCCCT CAGTGCATGG GAGGACAGAC TGGGTGGCCA AGTATGGAGG CCACCGGTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011512925.1
CDS159..1538
Misc Feature(1)159..443(+)
Translation
Position Chain Variation Link
2 2 a, g dbSNP:765270920
9 9 a, g dbSNP:759659345
17 17 c, t dbSNP:776770214
19 19 c, g dbSNP:771009179
24 24 a, g dbSNP:746881510
34 34 a, g dbSNP:367716586
39 39 -, tat dbSNP:777554686
41 41 a, t dbSNP:773172706
42 42 a, g dbSNP:771852650
64 64 -, g dbSNP:527236082
80 80 a, g dbSNP:200506656
87 87 c, t dbSNP:527236081
88 88 a, g dbSNP:387906648
106 106 a, t dbSNP:373520122
109 109 c, g dbSNP:369612015
110 110 a, g dbSNP:748742307
111 111 a, t dbSNP:779260712
121 121 a, c dbSNP:769213821
122 122 c, t dbSNP:749659651
123 123 a, g dbSNP:572867029
127 127 c, t dbSNP:558155479
132 132 a, g dbSNP:747216229
133 133 c, t dbSNP:750559316
138 138 a, g dbSNP:781377439
140 140 a, g dbSNP:376610958
144 144 a, g dbSNP:527236080
147 147 g, t dbSNP:141312468
151 151 -, g dbSNP:770308128
156 156 a, g dbSNP:56215624
172 172 a, g dbSNP:752496358
174 174 -, at dbSNP:746288738
190 190 c, t dbSNP:369797377
197 197 a, g dbSNP:759210520
206 206 a, g dbSNP:138302657
209 209 c, t dbSNP:765957619
213 213 a, g dbSNP:760103192
230 230 a, c dbSNP:143572116
237 237 a, t dbSNP:769287766
238 238 c, t dbSNP:182178532
265 265 c, g dbSNP:112257972
266 266 a, g dbSNP:770348792
269 269 a, c dbSNP:375351078
277 277 a, t dbSNP:530738750
280 280 g, t dbSNP:771061280
281 281 a, g dbSNP:747215914
291 291 a, g dbSNP:754415973
300 300 c, t dbSNP:111618569
301 301 a, g dbSNP:746596336
309 309 c, t dbSNP:779514800
310 310 a, g dbSNP:201114107
346 346 a, g dbSNP:371639894
347 347 a, t dbSNP:377107543
350 350 c, t dbSNP:778820480
358 358 a, g dbSNP:754864509
369 369 a, c, g dbSNP:368102323
377 377 c, t dbSNP:199949446
378 378 a, g dbSNP:199689074
389 389 c, t dbSNP:767172193
396 396 c, t dbSNP:761403711
405 405 c, t dbSNP:753314164
406 406 a, g dbSNP:765675052
409 409 c, t dbSNP:149734152
414 414 a, g dbSNP:776892781
426 426 c, t dbSNP:756912445
434 434 c, t dbSNP:55950618
442 442 g, t dbSNP:763618857
443 443 g, t dbSNP:759984800
453 453 c, g dbSNP:754349931
455 455 a, g dbSNP:766693820
458 458 a, g dbSNP:147570352
462 462 a, g dbSNP:200901108
469 469 c, t dbSNP:773485283
470 470 a, g dbSNP:772299314
472 472 c, g dbSNP:761959677
493 493 a, t dbSNP:774449472
501 501 a, g dbSNP:199921765
502 502 a, t dbSNP:768757013
510 510 c, g dbSNP:186218889
511 511 g, t dbSNP:775490393
520 520 c, t dbSNP:769534346
532 532 c, g dbSNP:745670820
538 538 -, t dbSNP:777909571
539 539 a, c dbSNP:781065923
540 540 a, g dbSNP:757000356
551 551 c, g dbSNP:746612091
558 558 a, c dbSNP:762412793
559 559 c, g, t dbSNP:200641218
565 565 c, t dbSNP:371971492
566 566 a, g dbSNP:145014649
567 567 g, t dbSNP:367903138
572 572 a, c dbSNP:750794210
575 575 c, t dbSNP:767830843
576 576 a, g dbSNP:201812469
578 578 g, t dbSNP:17579447
579 579 c, t dbSNP:774598986
585 585 g, t dbSNP:764142857
593 593 -, acccca dbSNP:758224960
596 596 c, t dbSNP:763072144
598 598 -, a dbSNP:748139115
602 602 a, c dbSNP:775374397
615 615 c, g dbSNP:769859243
626 626 a, g dbSNP:139604404
629 629 c, g dbSNP:776588742
631 631 c, t dbSNP:770829149
635 635 c, g dbSNP:746701788
637 637 c, t dbSNP:371774117
638 638 a, g dbSNP:757905846
640 640 c, t dbSNP:747711453
642 642 c, t dbSNP:778223936
644 644 c, g dbSNP:756648303
647 647 c, g dbSNP:750884327
651 651 a, g dbSNP:767905978
653 653 c, t dbSNP:199694073
654 654 a, g dbSNP:751783623
660 660 c, t dbSNP:200300453
662 662 a, g dbSNP:143424442
675 675 -, ca dbSNP:755434575
675 675 c, g dbSNP:763018137
684 684 a, g dbSNP:201558986
686 686 a, g dbSNP:775668388
689 689 a, c dbSNP:765357899
691 691 -, cc dbSNP:141174473
691 691 c, g, t dbSNP:776680313
692 692 a, g dbSNP:770917055
700 700 c, t dbSNP:201494324
701 701 c, g dbSNP:772828352
709 709 c, t dbSNP:771764863
712 712 a, g dbSNP:747716951
717 717 a, g dbSNP:778510807
719 719 c, t dbSNP:768120333
728 728 c, g dbSNP:150744360
730 730 c, t dbSNP:781652966
737 737 a, g dbSNP:753029772
742 742 a, g dbSNP:55773478
754 754 c, g dbSNP:755195964
767 767 -, t dbSNP:753698504
774 774 c, t dbSNP:753974545
775 775 a, g, t dbSNP:62000445
779 779 c, g dbSNP:750310318
782 782 c, t dbSNP:551658772
788 788 a, t dbSNP:767409599
796 796 a, c dbSNP:200533678
806 806 c, t dbSNP:148549681
811 811 a, g dbSNP:763737966
819 819 c, t dbSNP:762551107
820 820 c, t dbSNP:144848825
829 829 a, g dbSNP:200944697
836 836 c, t dbSNP:115185609
837 837 a, g dbSNP:773588644
838 838 a, g dbSNP:549436795
840 840 g, t dbSNP:748539209
841 841 c, t dbSNP:779355214
853 853 a, g dbSNP:755285965
857 857 g, t dbSNP:137918175
861 861 a, c dbSNP:780233328
868 868 a, g dbSNP:139003494
869 869 c, t dbSNP:750428776
879 879 a, g dbSNP:767365219
882 882 a, t dbSNP:757149659
887 887 a, g dbSNP:751385674
894 894 g, t dbSNP:201502742
896 896 g, t dbSNP:760421859
902 902 a, g dbSNP:775091188
906 906 c, t dbSNP:764870230
907 907 a, g dbSNP:758956117
908 908 c, g dbSNP:762462087
921 921 c, g dbSNP:556048925
922 922 c, t dbSNP:769213984
929 929 a, c dbSNP:763420270
932 932 c, t dbSNP:775852553
933 933 c, t dbSNP:769938480
938 938 a, c dbSNP:746051642
943 943 c, t dbSNP:200057744
945 945 a, g dbSNP:193132857
946 946 a, c dbSNP:547372600
947 947 -, ag dbSNP:762837543
950 950 a, g dbSNP:747015059
951 951 -, g dbSNP:777144918
957 957 a, g dbSNP:777674662
972 972 a, g dbSNP:376120162
973 973 c, t dbSNP:146071625
978 978 a, g, t dbSNP:754612195
995 995 c, t dbSNP:184920692
996 996 a, g dbSNP:149006687
1003 1003 -, tgtc dbSNP:771209345
1005 1005 a, g dbSNP:760262193
1008 1008 a, g dbSNP:369794041
1010 1010 a, t dbSNP:749890675
1025 1025 a, g dbSNP:764699614
1027 1027 a, g dbSNP:763510409
1040 1040 a, g dbSNP:775738175
1044 1044 a, g dbSNP:770170708
1045 1045 g, t dbSNP:759716806
1051 1051 c, t dbSNP:776906266
1055 1055 a, g dbSNP:779438081
1059 1059 a, g dbSNP:776804120
1071 1071 -, aag dbSNP:375070639
1071 1071 a, g dbSNP:766593207
1072 1072 a, c, t dbSNP:773304876
1074 1074 g, t dbSNP:772119574
1076 1076 a, g dbSNP:748079104
1090 1090 -, g dbSNP:774717726
1108 1108 c, t dbSNP:752021255
1110 1110 -, c dbSNP:748216114
1112 1112 a, c, g dbSNP:375261960
1116 1116 c, t dbSNP:779951600
1121 1121 c, t dbSNP:755850716
1128 1128 g, t dbSNP:745490858
1149 1149 c, t dbSNP:567083
1151 1151 c, t dbSNP:756785609
1153 1153 c, t dbSNP:192591726
1159 1159 c, t dbSNP:765787091
1183 1183 a, c dbSNP:755373628
1184 1184 c, t dbSNP:754233012
1209 1209 a, g dbSNP:374286792
1211 1211 a, c dbSNP:745580809
1212 1212 a, g dbSNP:769238012
1228 1228 a, g dbSNP:371455796
1235 1235 c, t dbSNP:747805700
1262 1262 a, g dbSNP:552111002
1287 1287 c, t dbSNP:761680845
1289 1289 a, c dbSNP:186643840
1291 1291 a, g dbSNP:761897291
1304 1304 c, t dbSNP:751678907
1306 1306 c, t dbSNP:79544660
1308 1308 c, t dbSNP:371838435
1313 1313 c, t dbSNP:775561335
1314 1314 c, t dbSNP:56217305
1323 1323 a, c dbSNP:759417469
1324 1324 a, g dbSNP:776221542
1331 1331 a, g dbSNP:770648753
1332 1332 a, c dbSNP:746594889
1334 1334 c, g dbSNP:556910057
1346 1346 a, g dbSNP:111281539
1353 1353 c, t dbSNP:200319110
1366 1366 c, t dbSNP:771453698
1369 1369 a, g dbSNP:200866765
1376 1376 a, g dbSNP:143121053
1378 1378 c, t dbSNP:756558713
1379 1379 c, t dbSNP:142308835
1394 1394 a, g dbSNP:770320899
1403 1403 a, t dbSNP:757841911
1406 1406 a, c, t dbSNP:570463236
1409 1409 -, tcgg dbSNP:760121453
1410 1410 c, t dbSNP:199594233
1411 1411 a, g dbSNP:368535018
1425 1425 c, g dbSNP:201628941
1428 1428 c, t dbSNP:553591095
1429 1429 a, g dbSNP:758636733
1432 1432 c, t dbSNP:752846705
1438 1438 a, c dbSNP:371429892
1440 1440 c, g dbSNP:147251038
1441 1441 c, g dbSNP:753694997
1442 1442 c, t dbSNP:766252727
1446 1446 a, g dbSNP:760381134
1453 1453 a, c dbSNP:180826550
1454 1454 a, c dbSNP:767167233
1462 1462 g, t dbSNP:761401422
1466 1466 a, g dbSNP:773894456
1470 1470 c, t dbSNP:768134966
1471 1471 a, g, t dbSNP:777002916
1479 1479 c, g, t dbSNP:747349238
1486 1486 c, t dbSNP:778235109
1490 1490 -, ag dbSNP:754210226
1496 1496 c, t dbSNP:126405
1497 1497 a, g dbSNP:748349220
1499 1499 a, g dbSNP:537862624
1501 1501 a, g dbSNP:377372773
1512 1512 a, g dbSNP:567613995
1513 1513 g, t dbSNP:766229047
1533 1533 c, t dbSNP:375794836
1534 1534 a, c, g dbSNP:767273634
1545 1545 a, g dbSNP:370549111
1549 1549 g, t dbSNP:773982305
1550 1550 a, g dbSNP:377201252
1558 1558 -, ctac dbSNP:766869339
1571 1571 c, t dbSNP:372487961
1572 1572 c, t dbSNP:78074137
1573 1573 a, g dbSNP:376336149
1574 1574 a, t dbSNP:747511670
1575 1575 a, c dbSNP:773751765
1581 1581 c, t dbSNP:373416620
1588 1588 c, g dbSNP:369955868
1589 1589 c, t dbSNP:560376669
1638 1638 a, g dbSNP:373312456
1658 1658 c, g dbSNP:536214552
1672 1672 c, g dbSNP:552039665
1710 1710 c, g dbSNP:753480982
1726 1726 c, t dbSNP:3734395
1764 1764 a, g dbSNP:547465110
1816 1816 a, t dbSNP:544885539
1817 1817 a, c dbSNP:575950279
1821 1821 c, g dbSNP:200154458
1822 1822 c, t dbSNP:542247606
1830 1830 a, g dbSNP:753241358
1850 1850 -, a dbSNP:769006848
1869 1869 -, ta dbSNP:558307147
1915 1915 c, t dbSNP:572139433
1968 1968 c, t dbSNP:763523138
2002 2002 c, g dbSNP:553427676
2028 2028 c, t dbSNP:538465920
2124 2124 -, t dbSNP:397705840
2139 2139 -, t, tt dbSNP:60516370
2139 2139 g, t dbSNP:75280442
2140 2140 g, t dbSNP:76389200
2141 2141 a, g dbSNP:376366712
2145 2145 a, g dbSNP:577379885
2159 2159 c, g dbSNP:527434856

Target ORF information:

RefSeq Version XM_011514623
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens male germ cell-associated kinase (MAK), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011514623

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCTGAAC TCTATATGTT AAGGCCACTT TTCCCAGGGA CAAGTGAGGT CGATGAAATC 
TTTAAAATTT GCCAAGTTTT AGGGACTCCC AAAAAAAGTG ACTGGCCAGA AGGATACCAG
CTGGCATCCT CTATGAACTT CCGTTTTCCC CAGTGTGTTC CTATAAACTT AAAAACTCTT
ATTCCCAATG CCAGTAATGA AGCTATTCAG CTCATGACCG AAATGTTGAA TTGGGATCCA
AAGAAACGAC CGACAGCAAG CCAGGCATTG AAACACCCAT ATTTTCAAGT TGGTCAGGTA
TTAGGCCCTT CGTCAAATCA TCTGGAATCA AAACAGTCTT TAAATAAGCA GCTGCAACCA
TTAGAATCAA AGCCATCTTT AGTTGAGGTA GAGCCTAAGC CTCTGCCGGA TATAATCGAT
CAGGTTGTTG GACAACCCCA GCCAAAAACT AGCCAGCAGC CACTGCAGCC CATTCAGCCG
CCACAGAACC TGAGCGTCCA GCAACCTCCA AAGCAACAGA GTCAGGAGAA ACCGCCACAA
ACGCTATTCC CGAGCATCGT CAAAAACATG CCAACTAAGC CAAATGGCAC ACTGAGTCAT
AAAAGTGGTA GGAGGCGTTG GGGTCAGACT ATCTTCAAGT CTGGAGATAG CTGGGAAGAG
TTGGAGGACT ATGATTTCGG AGCCTCCCAT TCCAAGAAGC CAAGCATGGG TGTTTTTAAA
GAAAAAAGGA AAAAAGATTC TCCATTTCGG CTTCCAGAGC CAGTACCCTC AGGCTCCAAC
CACTCGACAG GGGAAAACAA GAGCTTACCT GCTGTTACTT CCCTAAAATC TGATTCCGAA
TTGTCAACTG CTCCAACCTC TAAACAGTAC TACTTGAAAC AATCAAGATA TCTTCCAGGT
GTGAATCCCA AGAAGGTGTC CTTGATAGCC AGTGGAAAGG AAATAAACCC CCACACTTGG
AGCAACCAGT TATTCCCCAA GTCACTGGGA CCCGTTGGGG CAGAACTTGC TTTCAAAAGG
AGCAATGCAG AAGAAAGCAT AATTAAACCA ATCGAAAAAC TATCATGCAA TGAAACTTTT
CCTGAAAAAT TAGAGGACCC ACAAGGAAAT CTTGGAAGTT ATGCTACTTA CAATCAGTCA
GGATATATTC CTTCCTTTCT CAAAAAAGAA GTGCAGTCAG CTGGCCAGAG GATCCACTTA
GCACCTCTCA ATGCAACGGC TTCAGAATAT ACCTGGAACA CAAAAACTGG TCGGGGGCAG
TTTTCAGGAC GTACTTATAA TCCTACAGCA AAAAACCTAA ATATTGTGAA CCGTGCACAG
CCCATTCCCT CAGTGCATGG GAGGACAGAC TGGGTGGCCA AGTATGGAGG CCACCGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58834
Accession Version XM_011514624.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1167bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase MAK isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007592.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACCGAAA TGTTGAATTG GGATCCAAAG AAACGACCGA CAGCAAGCCA GGCATTGAAA 
CACCCATATT TTCAAGTTGG TCAGGTATTA GGCCCTTCGT CAAATCATCT GGAATCAAAA
CAGTCTTTAA ATAAGCAGCT GCAACCATTA GAATCAAAGC CATCTTTAGT TGAGGTAGAG
CCTAAGCCTC TGCCGGATAT AATCGATCAG GTTGTTGGAC AACCCCAGCC AAAAACTAGC
CAGCAGCCAC TGCAGCCCAT TCAGCCGCCA CAGAACCTGA GCGTCCAGCA ACCTCCAAAG
CAACAGAGTC AGGAGAAACC GCCACAAACG CTATTCCCGA GCATCGTCAA AAACATGCCA
ACTAAGCCAA ATGGCACACT GAGTCATAAA AGTGGTAGGA GGCGTTGGGG TCAGACTATC
TTCAAGTCTG GAGATAGCTG GGAAGAGTTG GAGGACTATG ATTTCGGAGC CTCCCATTCC
AAGAAGCCAA GCATGGGTGT TTTTAAAGAA AAAAGGAAAA AAGATTCTCC ATTTCGGCTT
CCAGAGCCAG TACCCTCAGG CTCCAACCAC TCGACAGGGG AAAACAAGAG CTTACCTGCT
GTTACTTCCC TAAAATCTGA TTCCGAATTG TCAACTGCTC CAACCTCTAA ACAGTACTAC
TTGAAACAAT CAAGATATCT TCCAGGTGTG AATCCCAAGA AGGTGTCCTT GATAGCCAGT
GGAAAGGAAA TAAACCCCCA CACTTGGAGC AACCAGTTAT TCCCCAAGTC ACTGGGACCC
GTTGGGGCAG AACTTGCTTT CAAAAGGAGC AATGCAGAAG AAAGCATAAT TAAACCAATC
GAAAAACTAT CATGCAATGA AACTTTTCCT GAAAAATTAG AGGACCCACA AGGAAATCTT
GGAAGTTATG CTACTTACAA TCAGTCAGGA TATATTCCTT CCTTTCTCAA AAAAGAAGTG
CAGTCAGCTG GCCAGAGGAT CCACTTAGCA CCTCTCAATG CAACGGCTTC AGAATATACC
TGGAACACAA AAACTGGTCG GGGGCAGTTT TCAGGACGTA CTTATAATCC TACAGCAAAA
AACCTAAATA TTGTGAACCG TGCACAGCCC ATTCCCTCAG TGCATGGGAG GACAGACTGG
GTGGCCAAGT ATGGAGGCCA CCGGTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011512926.1
CDS105..1271
Misc Feature(1)105..176(+)
Translation
Position Chain Variation Link
3 3 a, g dbSNP:752496358
5 5 -, at dbSNP:746288738
21 21 c, t dbSNP:369797377
28 28 a, g dbSNP:759210520
37 37 a, g dbSNP:138302657
40 40 c, t dbSNP:765957619
44 44 a, g dbSNP:760103192
61 61 a, c dbSNP:143572116
68 68 a, t dbSNP:769287766
69 69 c, t dbSNP:182178532
91 91 a, g dbSNP:754864509
102 102 a, c, g dbSNP:368102323
110 110 c, t dbSNP:199949446
111 111 a, g dbSNP:199689074
122 122 c, t dbSNP:767172193
129 129 c, t dbSNP:761403711
138 138 c, t dbSNP:753314164
139 139 a, g dbSNP:765675052
142 142 c, t dbSNP:149734152
147 147 a, g dbSNP:776892781
159 159 c, t dbSNP:756912445
167 167 c, t dbSNP:55950618
175 175 g, t dbSNP:763618857
176 176 g, t dbSNP:759984800
186 186 c, g dbSNP:754349931
188 188 a, g dbSNP:766693820
191 191 a, g dbSNP:147570352
195 195 a, g dbSNP:200901108
202 202 c, t dbSNP:773485283
203 203 a, g dbSNP:772299314
205 205 c, g dbSNP:761959677
226 226 a, t dbSNP:774449472
234 234 a, g dbSNP:199921765
235 235 a, t dbSNP:768757013
243 243 c, g dbSNP:186218889
244 244 g, t dbSNP:775490393
253 253 c, t dbSNP:769534346
265 265 c, g dbSNP:745670820
271 271 -, t dbSNP:777909571
272 272 a, c dbSNP:781065923
273 273 a, g dbSNP:757000356
284 284 c, g dbSNP:746612091
291 291 a, c dbSNP:762412793
292 292 c, g, t dbSNP:200641218
298 298 c, t dbSNP:371971492
299 299 a, g dbSNP:145014649
300 300 g, t dbSNP:367903138
305 305 a, c dbSNP:750794210
308 308 c, t dbSNP:767830843
309 309 a, g dbSNP:201812469
311 311 g, t dbSNP:17579447
312 312 c, t dbSNP:774598986
318 318 g, t dbSNP:764142857
326 326 -, acccca dbSNP:758224960
329 329 c, t dbSNP:763072144
331 331 -, a dbSNP:748139115
335 335 a, c dbSNP:775374397
348 348 c, g dbSNP:769859243
359 359 a, g dbSNP:139604404
362 362 c, g dbSNP:776588742
364 364 c, t dbSNP:770829149
368 368 c, g dbSNP:746701788
370 370 c, t dbSNP:371774117
371 371 a, g dbSNP:757905846
373 373 c, t dbSNP:747711453
375 375 c, t dbSNP:778223936
377 377 c, g dbSNP:756648303
380 380 c, g dbSNP:750884327
384 384 a, g dbSNP:767905978
386 386 c, t dbSNP:199694073
387 387 a, g dbSNP:751783623
393 393 c, t dbSNP:200300453
395 395 a, g dbSNP:143424442
408 408 -, ca dbSNP:755434575
408 408 c, g dbSNP:763018137
417 417 a, g dbSNP:201558986
419 419 a, g dbSNP:775668388
422 422 a, c dbSNP:765357899
424 424 -, cc dbSNP:141174473
424 424 c, g, t dbSNP:776680313
425 425 a, g dbSNP:770917055
433 433 c, t dbSNP:201494324
434 434 c, g dbSNP:772828352
442 442 c, t dbSNP:771764863
445 445 a, g dbSNP:747716951
450 450 a, g dbSNP:778510807
452 452 c, t dbSNP:768120333
461 461 c, g dbSNP:150744360
463 463 c, t dbSNP:781652966
470 470 a, g dbSNP:753029772
475 475 a, g dbSNP:55773478
487 487 c, g dbSNP:755195964
500 500 -, t dbSNP:753698504
507 507 c, t dbSNP:753974545
508 508 a, g, t dbSNP:62000445
512 512 c, g dbSNP:750310318
515 515 c, t dbSNP:551658772
521 521 a, t dbSNP:767409599
529 529 a, c dbSNP:200533678
539 539 c, t dbSNP:148549681
544 544 a, g dbSNP:763737966
552 552 c, t dbSNP:762551107
553 553 c, t dbSNP:144848825
562 562 a, g dbSNP:200944697
569 569 c, t dbSNP:115185609
570 570 a, g dbSNP:773588644
571 571 a, g dbSNP:549436795
573 573 g, t dbSNP:748539209
574 574 c, t dbSNP:779355214
586 586 a, g dbSNP:755285965
590 590 g, t dbSNP:137918175
594 594 a, c dbSNP:780233328
601 601 a, g dbSNP:139003494
602 602 c, t dbSNP:750428776
612 612 a, g dbSNP:767365219
615 615 a, t dbSNP:757149659
620 620 a, g dbSNP:751385674
627 627 g, t dbSNP:201502742
629 629 g, t dbSNP:760421859
635 635 a, g dbSNP:775091188
639 639 c, t