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MAN2B1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MAN2B1
Entrez Gene ID 4125
Full Name mannosidase, alpha, class 2B, member 1
Synonyms LAMAN, MANB
General protein information
Preferred Names
lysosomal alpha-mannosidase
Names
lysosomal alpha-mannosidase
mannosidase alpha-B
mannosidase, alpha B, lysosomal
lysosomal acid alpha-mannosidase
NP_000519.2
NP_001166969.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19p13.2

Summary This gene encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]. lac of sum
Disorder MIM:

609458

Disorder Html: Mannosidosis, alpha-, types I and II, 248500 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_005259913 XP_005259970 lysosomal alpha-mannosidase isoform X1
XM_011528017 XP_011526319 lysosomal alpha-mannosidase isoform X2
NM_000528 NP_000519 lysosomal alpha-mannosidase isoform 1 precursor
NM_001173498 NP_001166969 lysosomal alpha-mannosidase isoform 2 precursor



Homo sapiens (human) MAN2B1 NP_000519.2
Pan troglodytes (chimpanzee) MAN2B1 XP_003316198.2
Macaca mulatta (Rhesus monkey) LOC716173 XP_002801133.1
Canis lupus familiaris (dog) MAN2B1 XP_005632890.1
Bos taurus (cattle) MAN2B1 NP_776986.2
Mus musculus (house mouse) Man2b1 NP_034894.2
Rattus norvegicus (Norway rat) Man2b1 NP_955436.1
Danio rerio (zebrafish) man2b1 NP_001014354.1
Drosophila melanogaster (fruit fly) LM408 NP_609408.1
Caenorhabditis elegans aman-1 NP_508811.1
Arabidopsis thaliana (thale cress) AT5G13980 NP_196902.2
Xenopus (Silurana) tropicalis (western clawed frog) man2b1 NP_001072469.1


Related articles in PubMed

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAN2B1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAN2B1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu51603 XM_005259913 PREDICTED: Homo sapiens mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu58847 XM_011528017 PREDICTED: Homo sapiens mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu18492
NM_000528 Homo sapiens mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu16780 NM_001173498 Homo sapiens mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu51603
Clone ID Related Accession (Same CDS sequence) XM_005259913
Accession Version XM_005259913.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3039bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysosomal alpha-mannosidase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011295.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGGGCGCCT ACGCGCGGGC TTCGGGGGTC TGCGCTCGCG GCTGCCTGGA CTCAGCAGGC 
CCCTGGACCA TGTCCCGCGC CCTGCGGCCA CCGCTCCCGC CTCTCTGCTT TTTCCTTTTG
TTGCTGGCGG CTGCCGGTGC TCGGGCCGGG GGATACGAGA CATGCCCCAC AGTGCAGCCG
AACATGCTGA ACGTGCACCT GCTGCCTCAC ACACATGATG ACGTGGGCTG GCTCAAAACC
GTGGACCAGT ACTTTTATGG AATCAAGAAT GACATCCAGC ACGCCGGTGT GCAGTACATC
CTGGACTCGG TCATCTCTGC CTTGCTGGCA GATCCCACCC GTCGCTTCAT TTACGTGGAG
ATTGCCTTCT TCTCCCGTTG GTGGCACCAG CAGACAAATG CCACACAGGA AGTCGTGCGA
GACCTTGTGC GCCAGGGGCG CCTGGAGTTC GCCAATGGTG GCTGGGTGAT GAACGATGAG
GCAGCCACCC ACTACGGTGC CATCGTGGAC CAGATGACAC TTGGGCTGCG CTTTCTGGAG
GACACATTTG GCAATGATGG GCGACCCCGT GTGGCCTGGC ACATTGACCC CTTCGGCCAC
TCTCGGGAGC AGGCCTCGCT GTTTGCGCAG ATGGGCTTCG ACGGCTTCTT CTTTGGGCGC
CTTGATTATC AAGATAAGTG GGTACGGATG CAGAAGCTGG AGATGGAGCA GGTGTGGCGG
GCCAGCACCA GCCTGAAGCC CCCGACCGCG GACCTCTTCA CTGGTGTGCT TCCCAATGGT
TACAACCCGC CAAGGAATCT GTGCTGGGAT GTGCTGTGTG TCGATCAGCC GCTGGTGGAG
GACCCTCGCA GCCCCGAGTA CAACGCCAAG GAGCTGGTCG ATTACTTCCT AAATGTGGCC
ACTGCCCAGG GCCGGTATTA CCGCACCAAC CACACTGTGA TGACCATGGG CTCGGACTTC
CAATATGAGA ATGCCAACAT GTGGTTCAAG AACCTTGACA AGCTCATCCG GCTGGTAAAT
GCGCAGCAGC AGGCAAAAGG AAGCAGTGTC CATGTTCTCT ACTCCACCCC CGCTTGTTAC
CTCTGGGAGC TGAACAAGGC CAACCTCACC TGGTCAGTGA AACATGACGA CTTCTTCCCT
TACGCGGATG GCCCCCACCA GTTCTGGACC GGTTACTTTT CCAGTCGGCC GGCCCTCAAA
CGCTACGAGC GCCTCAGCTA CAACTTCCTG CAGGTGTGCA ACCAGCTGGA GGCGCTGGTG
GGCCTGGCGG CCAACGTGGG ACCCTATGGC TCCGGAGACA GTGCACCCCT CAATGAGGCG
ATGGCTGTGC TCCAGCATCA CGACGCCGTC AGCGGCACCT CCCGCCAGCA CGTGGCCAAC
GACTACGCGC GCCAGCTTGC GGCAGGCTGG GGGCCTTGCG AGGTTCTTCT GAGCAACGCG
CTGGCGCGGC TCAGAGGCTT CAAAGATCAC TTCACCTTTT GCCAACAGCT AAACATCAGC
ATCTGCCCGC TCAGCCAGAC GGCGGCGCGC TTCCAGGTCA TCGTTTATAA TCCCCTGGGG
CGGAAGGTGA ATTGGATGGT ACGGCTGCCG GTCAGCGAAG GCGTTTTCGT TGTGAAGGAC
CCCAATGGCA GGACAGTGCC CAGCGATGTG GTAATATTTC CCAGCTCAGA CAGCCAGGCG
CACCCTCCGG AGCTGCTGTT CTCAGCCTCA CTGCCCGCCC TGGGCTTCAG CACCTATTCA
GTAGCCCAGG TGCCTCGCTG GAAGCCCCAG GCCCGCGCAC CACAGCCCAT CCCCAGAAGA
TCCTGGTCCC CTGCTTTAAC CATCGAAAAT GAGCACATCC GGGCAACGTT TGATCCTGAC
ACAGGGCTGT TGATGGAGAT TATGAACATG AATCAGCAAC TCCTGCTGCC TGTTCGCCAG
ACCTTCTTCT GGTACAACGC CAGTATAGGT GACAACGAAA GTGACCAGGC CTCAGGTGCC
TACATCTTCA GACCCAACCA ACAGAAACCG CTGCCTGTGA GCCGCTGGGC TCAGATCCAC
CTGGTGAAGA CACCCTTGGT GCAGGAGGTG CACCAGAACT TCTCAGCTTG GTGTTCCCAG
GTGGTTCGCC TGTACCCAGG ACAGCGGCAC CTGGAGCTAG AGTGGTCGGT GGGGCCGATA
CCTGTGGGCG ACACCTGGGG GAAGGAGGTC ATCAGCCGTT TTGACACACC GCTGGAGACA
AAGGGACGCT TCTACACAGA CAGCAATGGC CGGGAGATCC TGGAGAGGAG GCGGGATTAT
CGACCCACCT GGAAACTGAA CCAGACGGAG CCCGTGGCAG GAAACTACTA TCCAGTCAAC
ACCCGGATTT ACATCACGGA TGGAAACATG CAGCTGACTG TGCTGACTGA CCGCTCCCAG
GGGGGCAGCA GCCTGAGAGA TGGCTCGCTG GAGCTCATGG TGCACCGAAG GCTGCTGAAG
GACGATGGAC GCGGAGTATC GGAGCCACTA ATGGAGAACG GGTCGGGGGC GTGGGTGCGA
GGGCGCCACC TGGTGCTGCT GGACACAGCC CAGGCTGCAG CCGCCGGACA CCGGCTCCTG
GCGGAGCAGG AGGTCCTGGC CCCTCAGGTG GTGCTGGCCC CGGGTGGCGG CGCCGCCTAC
AATCTCGGGG CTCCTCCGCG CACGCAGTTC TCAGGGCTGC GCAGGGACCT GCCGCCCTCG
GTGCACCTGC TCACGCTGGC CAGCTGGGGC CCCGAAATGG TGCTGCTGCG CTTGGAGCAC
CAGTTTGCCG TAGGAGAGGA TTCCGGACGT AACCTGAGCG CCCCCGTTAC CTTGAACTTG
AGGGACCTGT TCTCCACCTT CACCATCACC CGCCTGCAGG AGACCACGCT GGTGGCCAAC
CAGCTCCGCG AGGCAGCCTC CAGGCTCAAG TGGACAACAA ACACAGGCCC CACACCCCAC
CAAACTCCGT ACCAGCTGGA CCCGGCCAAC ATCACGCTGG AACCCATGGA AATCCGCACT
TTCCTGGCCT CAGTTCAATG GAAGGAGGTG GATGGTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005259970.1
CDS55..3093
Misc Feature(1)241..3057(+)
Misc Feature(2)241..1077(+)
Misc Feature(3)268..1011(+)
Misc Feature(4)274..783(+)
Misc Feature(5)451..453(+)
Misc Feature(6)640..1011(+)
Misc Feature(7)1216..1452(+)
Misc Feature(8)1585..3057(+)
Translation

Target ORF information:

RefSeq Version XM_005259913
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005259913

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGGGCGCCT ACGCGCGGGC TTCGGGGGTC TGCGCTCGCG GCTGCCTGGA CTCAGCAGGC 
CCCTGGACCA TGTCCCGCGC CCTGCGGCCA CCGCTCCCGC CTCTCTGCTT TTTCCTTTTG
TTGCTGGCGG CTGCCGGTGC TCGGGCCGGG GGATACGAGA CATGCCCCAC AGTGCAGCCG
AACATGCTGA ACGTGCACCT GCTGCCTCAC ACACATGATG ACGTGGGCTG GCTCAAAACC
GTGGACCAGT ACTTTTATGG AATCAAGAAT GACATCCAGC ACGCCGGTGT GCAGTACATC
CTGGACTCGG TCATCTCTGC CTTGCTGGCA GATCCCACCC GTCGCTTCAT TTACGTGGAG
ATTGCCTTCT TCTCCCGTTG GTGGCACCAG CAGACAAATG CCACACAGGA AGTCGTGCGA
GACCTTGTGC GCCAGGGGCG CCTGGAGTTC GCCAATGGTG GCTGGGTGAT GAACGATGAG
GCAGCCACCC ACTACGGTGC CATCGTGGAC CAGATGACAC TTGGGCTGCG CTTTCTGGAG
GACACATTTG GCAATGATGG GCGACCCCGT GTGGCCTGGC ACATTGACCC CTTCGGCCAC
TCTCGGGAGC AGGCCTCGCT GTTTGCGCAG ATGGGCTTCG ACGGCTTCTT CTTTGGGCGC
CTTGATTATC AAGATAAGTG GGTACGGATG CAGAAGCTGG AGATGGAGCA GGTGTGGCGG
GCCAGCACCA GCCTGAAGCC CCCGACCGCG GACCTCTTCA CTGGTGTGCT TCCCAATGGT
TACAACCCGC CAAGGAATCT GTGCTGGGAT GTGCTGTGTG TCGATCAGCC GCTGGTGGAG
GACCCTCGCA GCCCCGAGTA CAACGCCAAG GAGCTGGTCG ATTACTTCCT AAATGTGGCC
ACTGCCCAGG GCCGGTATTA CCGCACCAAC CACACTGTGA TGACCATGGG CTCGGACTTC
CAATATGAGA ATGCCAACAT GTGGTTCAAG AACCTTGACA AGCTCATCCG GCTGGTAAAT
GCGCAGCAGC AGGCAAAAGG AAGCAGTGTC CATGTTCTCT ACTCCACCCC CGCTTGTTAC
CTCTGGGAGC TGAACAAGGC CAACCTCACC TGGTCAGTGA AACATGACGA CTTCTTCCCT
TACGCGGATG GCCCCCACCA GTTCTGGACC GGTTACTTTT CCAGTCGGCC GGCCCTCAAA
CGCTACGAGC GCCTCAGCTA CAACTTCCTG CAGGTGTGCA ACCAGCTGGA GGCGCTGGTG
GGCCTGGCGG CCAACGTGGG ACCCTATGGC TCCGGAGACA GTGCACCCCT CAATGAGGCG
ATGGCTGTGC TCCAGCATCA CGACGCCGTC AGCGGCACCT CCCGCCAGCA CGTGGCCAAC
GACTACGCGC GCCAGCTTGC GGCAGGCTGG GGGCCTTGCG AGGTTCTTCT GAGCAACGCG
CTGGCGCGGC TCAGAGGCTT CAAAGATCAC TTCACCTTTT GCCAACAGCT AAACATCAGC
ATCTGCCCGC TCAGCCAGAC GGCGGCGCGC TTCCAGGTCA TCGTTTATAA TCCCCTGGGG
CGGAAGGTGA ATTGGATGGT ACGGCTGCCG GTCAGCGAAG GCGTTTTCGT TGTGAAGGAC
CCCAATGGCA GGACAGTGCC CAGCGATGTG GTAATATTTC CCAGCTCAGA CAGCCAGGCG
CACCCTCCGG AGCTGCTGTT CTCAGCCTCA CTGCCCGCCC TGGGCTTCAG CACCTATTCA
GTAGCCCAGG TGCCTCGCTG GAAGCCCCAG GCCCGCGCAC CACAGCCCAT CCCCAGAAGA
TCCTGGTCCC CTGCTTTAAC CATCGAAAAT GAGCACATCC GGGCAACGTT TGATCCTGAC
ACAGGGCTGT TGATGGAGAT TATGAACATG AATCAGCAAC TCCTGCTGCC TGTTCGCCAG
ACCTTCTTCT GGTACAACGC CAGTATAGGT GACAACGAAA GTGACCAGGC CTCAGGTGCC
TACATCTTCA GACCCAACCA ACAGAAACCG CTGCCTGTGA GCCGCTGGGC TCAGATCCAC
CTGGTGAAGA CACCCTTGGT GCAGGAGGTG CACCAGAACT TCTCAGCTTG GTGTTCCCAG
GTGGTTCGCC TGTACCCAGG ACAGCGGCAC CTGGAGCTAG AGTGGTCGGT GGGGCCGATA
CCTGTGGGCG ACACCTGGGG GAAGGAGGTC ATCAGCCGTT TTGACACACC GCTGGAGACA
AAGGGACGCT TCTACACAGA CAGCAATGGC CGGGAGATCC TGGAGAGGAG GCGGGATTAT
CGACCCACCT GGAAACTGAA CCAGACGGAG CCCGTGGCAG GAAACTACTA TCCAGTCAAC
ACCCGGATTT ACATCACGGA TGGAAACATG CAGCTGACTG TGCTGACTGA CCGCTCCCAG
GGGGGCAGCA GCCTGAGAGA TGGCTCGCTG GAGCTCATGG TGCACCGAAG GCTGCTGAAG
GACGATGGAC GCGGAGTATC GGAGCCACTA ATGGAGAACG GGTCGGGGGC GTGGGTGCGA
GGGCGCCACC TGGTGCTGCT GGACACAGCC CAGGCTGCAG CCGCCGGACA CCGGCTCCTG
GCGGAGCAGG AGGTCCTGGC CCCTCAGGTG GTGCTGGCCC CGGGTGGCGG CGCCGCCTAC
AATCTCGGGG CTCCTCCGCG CACGCAGTTC TCAGGGCTGC GCAGGGACCT GCCGCCCTCG
GTGCACCTGC TCACGCTGGC CAGCTGGGGC CCCGAAATGG TGCTGCTGCG CTTGGAGCAC
CAGTTTGCCG TAGGAGAGGA TTCCGGACGT AACCTGAGCG CCCCCGTTAC CTTGAACTTG
AGGGACCTGT TCTCCACCTT CACCATCACC CGCCTGCAGG AGACCACGCT GGTGGCCAAC
CAGCTCCGCG AGGCAGCCTC CAGGCTCAAG TGGACAACAA ACACAGGCCC CACACCCCAC
CAAACTCCGT ACCAGCTGGA CCCGGCCAAC ATCACGCTGG AACCCATGGA AATCCGCACT
TTCCTGGCCT CAGTTCAATG GAAGGAGGTG GATGGTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58847
Clone ID Related Accession (Same CDS sequence) XM_011528017
Accession Version XM_011528017.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1935bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysosomal alpha-mannosidase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011295.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGCGCAGGT CAGTGAAACA TGACGACTTC TTCCCTTACG CGGATGGCCC CCACCAGTTC 
TGGACCGGTT ACTTTTCCAG TCGGCCGGCC CTCAAACGCT ACGAGCGCCT CAGCTACAAC
TTCCTGCAGG TGTGCAACCA GCTGGAGGCG CTGGTGGGCC TGGCGGCCAA CGTGGGACCC
TATGGCTCCG GAGACAGTGC ACCCCTCAAT GAGGCGATGG CTGTGCTCCA GCATCACGAC
GCCGTCAGCG GCACCTCCCG CCAGCACGTG GCCAACGACT ACGCGCGCCA GCTTGCGGCA
GGCTGGGGGC CTTGCGAGGT TCTTCTGAGC AACGCGCTGG CGCGGCTCAG AGGCTTCAAA
GATCACTTCA CCTTTTGCCA ACAGCTAAAC ATCAGCATCT GCCCGCTCAG CCAGACGGCG
GCGCGCTTCC AGGTCATCGT TTATAATCCC CTGGGGCGGA AGGTGAATTG GATGGTACGG
CTGCCGGTCA GCGAAGGCGT TTTCGTTGTG AAGGACCCCA ATGGCAGGAC AGTGCCCAGC
GATGTGGTAA TATTTCCCAG CTCAGACAGC CAGGCGCACC CTCCGGAGCT GCTGTTCTCA
GCCTCACTGC CCGCCCTGGG CTTCAGCACC TATTCAGTAG CCCAGGTGCC TCGCTGGAAG
CCCCAGGCCC GCGCACCACA GCCCATCCCC AGAAGATCCT GGTCCCCTGC TTTAACCATC
GAAAATGAGC ACATCCGGGC AACGTTTGAT CCTGACACAG GGCTGTTGAT GGAGATTATG
AACATGAATC AGCAACTCCT GCTGCCTGTT CGCCAGACCT TCTTCTGGTA CAACGCCAGT
ATAGGTGACA ACGAAAGTGA CCAGGCCTCA GGTGCCTACA TCTTCAGACC CAACCAACAG
AAACCGCTGC CTGTGAGCCG CTGGGCTCAG ATCCACCTGG TGAAGACACC CTTGGTGCAG
GAGGTGCACC AGAACTTCTC AGCTTGGTGT TCCCAGGTGG TTCGCCTGTA CCCAGGACAG
CGGCACCTGG AGCTAGAGTG GTCGGTGGGG CCGATACCTG TGGGCGACAC CTGGGGGAAG
GAGGTCATCA GCCGTTTTGA CACACCGCTG GAGACAAAGG GACGCTTCTA CACAGACAGC
AATGGCCGGG AGATCCTGGA GAGGAGGCGG GATTATCGAC CCACCTGGAA ACTGAACCAG
ACGGAGCCCG TGGCAGGAAA CTACTATCCA GTCAACACCC GGATTTACAT CACGGATGGA
AACATGCAGC TGACTGTGCT GACTGACCGC TCCCAGGGGG GCAGCAGCCT GAGAGATGGC
TCGCTGGAGC TCATGGTGCA CCGAAGGCTG CTGAAGGACG ATGGACGCGG AGTATCGGAG
CCACTAATGG AGAACGGGTC GGGGGCGTGG GTGCGAGGGC GCCACCTGGT GCTGCTGGAC
ACAGCCCAGG CTGCAGCCGC CGGACACCGG CTCCTGGCGG AGCAGGAGGT CCTGGCCCCT
CAGGTGGTGC TGGCCCCGGG TGGCGGCGCC GCCTACAATC TCGGGGCTCC TCCGCGCACG
CAGTTCTCAG GGCTGCGCAG GGACCTGCCG CCCTCGGTGC ACCTGCTCAC GCTGGCCAGC
TGGGGCCCCG AAATGGTGCT GCTGCGCTTG GAGCACCAGT TTGCCGTAGG AGAGGATTCC
GGACGTAACC TGAGCGCCCC CGTTACCTTG AACTTGAGGG ACCTGTTCTC CACCTTCACC
ATCACCCGCC TGCAGGAGAC CACGCTGGTG GCCAACCAGC TCCGCGAGGC AGCCTCCAGG
CTCAAGTGGA CAACAAACAC AGGCCCCACA CCCCACCAAA CTCCGTACCA GCTGGACCCG
GCCAACATCA CGCTGGAACC CATGGAAATC CGCACTTTCC TGGCCTCAGT TCAATGGAAG
GAGGTGGATG GTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011526319.1
CDS160..2094
Misc Feature(1)217..453(+)
Misc Feature(2)586..2058(+)
Translation

Target ORF information:

RefSeq Version XM_011528017
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011528017

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGCGCAGGT CAGTGAAACA TGACGACTTC TTCCCTTACG CGGATGGCCC CCACCAGTTC 
TGGACCGGTT ACTTTTCCAG TCGGCCGGCC CTCAAACGCT ACGAGCGCCT CAGCTACAAC
TTCCTGCAGG TGTGCAACCA GCTGGAGGCG CTGGTGGGCC TGGCGGCCAA CGTGGGACCC
TATGGCTCCG GAGACAGTGC ACCCCTCAAT GAGGCGATGG CTGTGCTCCA GCATCACGAC
GCCGTCAGCG GCACCTCCCG CCAGCACGTG GCCAACGACT ACGCGCGCCA GCTTGCGGCA
GGCTGGGGGC CTTGCGAGGT TCTTCTGAGC AACGCGCTGG CGCGGCTCAG AGGCTTCAAA
GATCACTTCA CCTTTTGCCA ACAGCTAAAC ATCAGCATCT GCCCGCTCAG CCAGACGGCG
GCGCGCTTCC AGGTCATCGT TTATAATCCC CTGGGGCGGA AGGTGAATTG GATGGTACGG
CTGCCGGTCA GCGAAGGCGT TTTCGTTGTG AAGGACCCCA ATGGCAGGAC AGTGCCCAGC
GATGTGGTAA TATTTCCCAG CTCAGACAGC CAGGCGCACC CTCCGGAGCT GCTGTTCTCA
GCCTCACTGC CCGCCCTGGG CTTCAGCACC TATTCAGTAG CCCAGGTGCC TCGCTGGAAG
CCCCAGGCCC GCGCACCACA GCCCATCCCC AGAAGATCCT GGTCCCCTGC TTTAACCATC
GAAAATGAGC ACATCCGGGC AACGTTTGAT CCTGACACAG GGCTGTTGAT GGAGATTATG
AACATGAATC AGCAACTCCT GCTGCCTGTT CGCCAGACCT TCTTCTGGTA CAACGCCAGT
ATAGGTGACA ACGAAAGTGA CCAGGCCTCA GGTGCCTACA TCTTCAGACC CAACCAACAG
AAACCGCTGC CTGTGAGCCG CTGGGCTCAG ATCCACCTGG TGAAGACACC CTTGGTGCAG
GAGGTGCACC AGAACTTCTC AGCTTGGTGT TCCCAGGTGG TTCGCCTGTA CCCAGGACAG
CGGCACCTGG AGCTAGAGTG GTCGGTGGGG CCGATACCTG TGGGCGACAC CTGGGGGAAG
GAGGTCATCA GCCGTTTTGA CACACCGCTG GAGACAAAGG GACGCTTCTA CACAGACAGC
AATGGCCGGG AGATCCTGGA GAGGAGGCGG GATTATCGAC CCACCTGGAA ACTGAACCAG
ACGGAGCCCG TGGCAGGAAA CTACTATCCA GTCAACACCC GGATTTACAT CACGGATGGA
AACATGCAGC TGACTGTGCT GACTGACCGC TCCCAGGGGG GCAGCAGCCT GAGAGATGGC
TCGCTGGAGC TCATGGTGCA CCGAAGGCTG CTGAAGGACG ATGGACGCGG AGTATCGGAG
CCACTAATGG AGAACGGGTC GGGGGCGTGG GTGCGAGGGC GCCACCTGGT GCTGCTGGAC
ACAGCCCAGG CTGCAGCCGC CGGACACCGG CTCCTGGCGG AGCAGGAGGT CCTGGCCCCT
CAGGTGGTGC TGGCCCCGGG TGGCGGCGCC GCCTACAATC TCGGGGCTCC TCCGCGCACG
CAGTTCTCAG GGCTGCGCAG GGACCTGCCG CCCTCGGTGC ACCTGCTCAC GCTGGCCAGC
TGGGGCCCCG AAATGGTGCT GCTGCGCTTG GAGCACCAGT TTGCCGTAGG AGAGGATTCC
GGACGTAACC TGAGCGCCCC CGTTACCTTG AACTTGAGGG ACCTGTTCTC CACCTTCACC
ATCACCCGCC TGCAGGAGAC CACGCTGGTG GCCAACCAGC TCCGCGAGGC AGCCTCCAGG
CTCAAGTGGA CAACAAACAC AGGCCCCACA CCCCACCAAA CTCCGTACCA GCTGGACCCG
GCCAACATCA CGCTGGAACC CATGGAAATC CGCACTTTCC TGGCCTCAGT TCAATGGAAG
GAGGTGGATG GTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18492
Clone ID Related Accession (Same CDS sequence) NM_000528
Accession Version NM_000528.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product lysosomal alpha-mannosidase isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB237901.1, BC000736.2 and BM982008.1. This sequence is a reference standard in the RefSeqGene project. On May 9, 2008 this sequence version replaced gi:51873063. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK291572.1, BC000736.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGGGCGCCT ACGCGCGGGC TTCGGGGGTC TGCGCTCGCG GCTGCCTGGA CTCAGCAGGC 
CCCTGGACCA TGTCCCGCGC CCTGCGGCCA CCGCTCCCGC CTCTCTGCTT TTTCCTTTTG
TTGCTGGCGG CTGCCGGTGC TCGGGCCGGG GGATACGAGA CATGCCCCAC AGTGCAGCCG
AACATGCTGA ACGTGCACCT GCTGCCTCAC ACACATGATG ACGTGGGCTG GCTCAAAACC
GTGGACCAGT ACTTTTATGG AATCAAGAAT GACATCCAGC ACGCCGGTGT GCAGTACATC
CTGGACTCGG TCATCTCTGC CTTGCTGGCA GATCCCACCC GTCGCTTCAT TTACGTGGAG
ATTGCCTTCT TCTCCCGTTG GTGGCACCAG CAGACAAATG CCACACAGGA AGTCGTGCGA
GACCTTGTGC GCCAGGGGCG CCTGGAGTTC GCCAATGGTG GCTGGGTGAT GAACGATGAG
GCAGCCACCC ACTACGGTGC CATCGTGGAC CAGATGACAC TTGGGCTGCG CTTTCTGGAG
GACACATTTG GCAATGATGG GCGACCCCGT GTGGCCTGGC ACATTGACCC CTTCGGCCAC
TCTCGGGAGC AGGCCTCGCT GTTTGCGCAG ATGGGCTTCG ACGGCTTCTT CTTTGGGCGC
CTTGATTATC AAGATAAGTG GGTACGGATG CAGAAGCTGG AGATGGAGCA GGTGTGGCGG
GCCAGCACCA GCCTGAAGCC CCCGACCGCG GACCTCTTCA CTGGTGTGCT TCCCAATGGT
TACAACCCGC CAAGGAATCT GTGCTGGGAT GTGCTGTGTG TCGATCAGCC GCTGGTGGAG
GACCCTCGCA GCCCCGAGTA CAACGCCAAG GAGCTGGTCG ATTACTTCCT AAATGTGGCC
ACTGCCCAGG GCCGGTATTA CCGCACCAAC CACACTGTGA TGACCATGGG CTCGGACTTC
CAATATGAGA ATGCCAACAT GTGGTTCAAG AACCTTGACA AGCTCATCCG GCTGGTAAAT
GCGCAGCAGG CAAAAGGAAG CAGTGTCCAT GTTCTCTACT CCACCCCCGC TTGTTACCTC
TGGGAGCTGA ACAAGGCCAA CCTCACCTGG TCAGTGAAAC ATGACGACTT CTTCCCTTAC
GCGGATGGCC CCCACCAGTT CTGGACCGGT TACTTTTCCA GTCGGCCGGC CCTCAAACGC
TACGAGCGCC TCAGCTACAA CTTCCTGCAG GTGTGCAACC AGCTGGAGGC GCTGGTGGGC
CTGGCGGCCA ACGTGGGACC CTATGGCTCC GGAGACAGTG CACCCCTCAA TGAGGCGATG
GCTGTGCTCC AGCATCACGA CGCCGTCAGC GGCACCTCCC GCCAGCACGT GGCCAACGAC
TACGCGCGCC AGCTTGCGGC AGGCTGGGGG CCTTGCGAGG TTCTTCTGAG CAACGCGCTG
GCGCGGCTCA GAGGCTTCAA AGATCACTTC ACCTTTTGCC AACAGCTAAA CATCAGCATC
TGCCCGCTCA GCCAGACGGC GGCGCGCTTC CAGGTCATCG TTTATAATCC CCTGGGGCGG
AAGGTGAATT GGATGGTACG GCTGCCGGTC AGCGAAGGCG TTTTCGTTGT GAAGGACCCC
AATGGCAGGA CAGTGCCCAG CGATGTGGTA ATATTTCCCA GCTCAGACAG CCAGGCGCAC
CCTCCGGAGC TGCTGTTCTC AGCCTCACTG CCCGCCCTGG GCTTCAGCAC CTATTCAGTA
GCCCAGGTGC CTCGCTGGAA GCCCCAGGCC CGCGCACCAC AGCCCATCCC CAGAAGATCC
TGGTCCCCTG CTTTAACCAT CGAAAATGAG CACATCCGGG CAACGTTTGA TCCTGACACA
GGGCTGTTGA TGGAGATTAT GAACATGAAT CAGCAACTCC TGCTGCCTGT TCGCCAGACC
TTCTTCTGGT ACAACGCCAG TATAGGTGAC AACGAAAGTG ACCAGGCCTC AGGTGCCTAC
ATCTTCAGAC CCAACCAACA GAAACCGCTG CCTGTGAGCC GCTGGGCTCA GATCCACCTG
GTGAAGACAC CCTTGGTGCA GGAGGTGCAC CAGAACTTCT CAGCTTGGTG TTCCCAGGTG
GTTCGCCTGT ACCCAGGACA GCGGCACCTG GAGCTAGAGT GGTCGGTGGG GCCGATACCT
GTGGGCGACA CCTGGGGGAA GGAGGTCATC AGCCGTTTTG ACACACCGCT GGAGACAAAG
GGACGCTTCT ACACAGACAG CAATGGCCGG GAGATCCTGG AGAGGAGGCG GGATTATCGA
CCCACCTGGA AACTGAACCA GACGGAGCCC GTGGCAGGAA ACTACTATCC AGTCAACACC
CGGATTTACA TCACGGATGG AAACATGCAG CTGACTGTGC TGACTGACCG CTCCCAGGGG
GGCAGCAGCC TGAGAGATGG CTCGCTGGAG CTCATGGTGC ACCGAAGGCT GCTGAAGGAC
GATGGACGCG GAGTATCGGA GCCACTAATG GAGAACGGGT CGGGGGCGTG GGTGCGAGGG
CGCCACCTGG TGCTGCTGGA CACAGCCCAG GCTGCAGCCG CCGGACACCG GCTCCTGGCG
GAGCAGGAGG TCCTGGCCCC TCAGGTGGTG CTGGCCCCGG GTGGCGGCGC CGCCTACAAT
CTCGGGGCTC CTCCGCGCAC GCAGTTCTCA GGGCTGCGCA GGGACCTGCC GCCCTCGGTG
CACCTGCTCA CGCTGGCCAG CTGGGGCCCC GAAATGGTGC TGCTGCGCTT GGAGCACCAG
TTTGCCGTAG GAGAGGATTC CGGACGTAAC CTGAGCGCCC CCGTTACCTT GAACTTGAGG
GACCTGTTCT CCACCTTCAC CATCACCCGC CTGCAGGAGA CCACGCTGGT GGCCAACCAG
CTCCGCGAGG CAGCCTCCAG GCTCAAGTGG ACAACAAACA CAGGCCCCAC ACCCCACCAA
ACTCCGTACC AGCTGGACCC GGCCAACATC ACGCTGGAAC CCATGGAAAT CCGCACTTTC
CTGGCCTCAG TTCAATGGAA GGAGGTGGAT GGTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000519.2
CDS77..3112
Misc Feature(1)71..73(+)
Misc Feature(2)263..3076(+)
Misc Feature(3)263..1099(+)
Misc Feature(4)290..1033(+)
Misc Feature(5)296..805(+)
Misc Feature(6)473..475(+)
Misc Feature(7)662..1033(+)
Misc Feature(8)1004..1006(+)
Misc Feature(9)1109..1111(+)
Misc Feature(10)1235..1471(+)
Misc Feature(11)1361..1363(+)
Misc Feature(12)1604..3076(+)
Misc Feature(13)1877..1879(+)
Misc Feature(14)2720..2722(+)
Misc Feature(15)2864..2866(+)
Exon (1)1..235
Gene:MAN2B1
Gene Synonym:
Exon (2)236..338
Gene:MAN2B1
Gene Synonym:
Exon (3)339..512
Gene:MAN2B1
Gene Synonym:
Exon (4)513..706
Gene:MAN2B1
Gene Synonym:
Exon (5)707..839
Gene:MAN2B1
Gene Synonym:
Exon (6)840..985
Gene:MAN2B1
Gene Synonym:
Exon (7)986..1102
Gene:MAN2B1
Gene Synonym:
Exon (8)1103..1185
Gene:MAN2B1
Gene Synonym:
Exon (9)1186..1306
Gene:MAN2B1
Gene Synonym:
Exon (10)1307..1385
Gene:MAN2B1
Gene Synonym:
Exon (11)1386..1495
Gene:MAN2B1
Gene Synonym:
Exon (12)1496..1603
Gene:MAN2B1
Gene Synonym:
Exon (13)1604..1720
Gene:MAN2B1
Gene Synonym:
Exon (14)1721..1906
Gene:MAN2B1
Gene Synonym:
Exon (15)1907..2004
Gene:MAN2B1
Gene Synonym:
Exon (16)2005..2122
Gene:MAN2B1
Gene Synonym:
Exon (17)2123..2241
Gene:MAN2B1
Gene Synonym:
Exon (18)2242..2343
Gene:MAN2B1
Gene Synonym:
Exon (19)2344..2431
Gene:MAN2B1
Gene Synonym:
Exon (20)2432..2512
Gene:MAN2B1
Gene Synonym:
Exon (21)2513..2740
Gene:MAN2B1
Gene Synonym:
Exon (22)2741..2896
Gene:MAN2B1
Gene Synonym:
Exon (23)2897..2999
Gene:MAN2B1
Gene Synonym:
Exon (24)3000..3224
Gene:MAN2B1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000528
Organism Homo sapiens (human)
Definition Homo sapiens mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000528

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGGGCGCCT ACGCGCGGGC TTCGGGGGTC TGCGCTCGCG GCTGCCTGGA CTCAGCAGGC 
CCCTGGACCA TGTCCCGCGC CCTGCGGCCA CCGCTCCCGC CTCTCTGCTT TTTCCTTTTG
TTGCTGGCGG CTGCCGGTGC TCGGGCCGGG GGATACGAGA CATGCCCCAC AGTGCAGCCG
AACATGCTGA ACGTGCACCT GCTGCCTCAC ACACATGATG ACGTGGGCTG GCTCAAAACC
GTGGACCAGT ACTTTTATGG AATCAAGAAT GACATCCAGC ACGCCGGTGT GCAGTACATC
CTGGACTCGG TCATCTCTGC CTTGCTGGCA GATCCCACCC GTCGCTTCAT TTACGTGGAG
ATTGCCTTCT TCTCCCGTTG GTGGCACCAG CAGACAAATG CCACACAGGA AGTCGTGCGA
GACCTTGTGC GCCAGGGGCG CCTGGAGTTC GCCAATGGTG GCTGGGTGAT GAACGATGAG
GCAGCCACCC ACTACGGTGC CATCGTGGAC CAGATGACAC TTGGGCTGCG CTTTCTGGAG
GACACATTTG GCAATGATGG GCGACCCCGT GTGGCCTGGC ACATTGACCC CTTCGGCCAC
TCTCGGGAGC AGGCCTCGCT GTTTGCGCAG ATGGGCTTCG ACGGCTTCTT CTTTGGGCGC
CTTGATTATC AAGATAAGTG GGTACGGATG CAGAAGCTGG AGATGGAGCA GGTGTGGCGG
GCCAGCACCA GCCTGAAGCC CCCGACCGCG GACCTCTTCA CTGGTGTGCT TCCCAATGGT
TACAACCCGC CAAGGAATCT GTGCTGGGAT GTGCTGTGTG TCGATCAGCC GCTGGTGGAG
GACCCTCGCA GCCCCGAGTA CAACGCCAAG GAGCTGGTCG ATTACTTCCT AAATGTGGCC
ACTGCCCAGG GCCGGTATTA CCGCACCAAC CACACTGTGA TGACCATGGG CTCGGACTTC
CAATATGAGA ATGCCAACAT GTGGTTCAAG AACCTTGACA AGCTCATCCG GCTGGTAAAT
GCGCAGCAGG CAAAAGGAAG CAGTGTCCAT GTTCTCTACT CCACCCCCGC TTGTTACCTC
TGGGAGCTGA ACAAGGCCAA CCTCACCTGG TCAGTGAAAC ATGACGACTT CTTCCCTTAC
GCGGATGGCC CCCACCAGTT CTGGACCGGT TACTTTTCCA GTCGGCCGGC CCTCAAACGC
TACGAGCGCC TCAGCTACAA CTTCCTGCAG GTGTGCAACC AGCTGGAGGC GCTGGTGGGC
CTGGCGGCCA ACGTGGGACC CTATGGCTCC GGAGACAGTG CACCCCTCAA TGAGGCGATG
GCTGTGCTCC AGCATCACGA CGCCGTCAGC GGCACCTCCC GCCAGCACGT GGCCAACGAC
TACGCGCGCC AGCTTGCGGC AGGCTGGGGG CCTTGCGAGG TTCTTCTGAG CAACGCGCTG
GCGCGGCTCA GAGGCTTCAA AGATCACTTC ACCTTTTGCC AACAGCTAAA CATCAGCATC
TGCCCGCTCA GCCAGACGGC GGCGCGCTTC CAGGTCATCG TTTATAATCC CCTGGGGCGG
AAGGTGAATT GGATGGTACG GCTGCCGGTC AGCGAAGGCG TTTTCGTTGT GAAGGACCCC
AATGGCAGGA CAGTGCCCAG CGATGTGGTA ATATTTCCCA GCTCAGACAG CCAGGCGCAC
CCTCCGGAGC TGCTGTTCTC AGCCTCACTG CCCGCCCTGG GCTTCAGCAC CTATTCAGTA
GCCCAGGTGC CTCGCTGGAA GCCCCAGGCC CGCGCACCAC AGCCCATCCC CAGAAGATCC
TGGTCCCCTG CTTTAACCAT CGAAAATGAG CACATCCGGG CAACGTTTGA TCCTGACACA
GGGCTGTTGA TGGAGATTAT GAACATGAAT CAGCAACTCC TGCTGCCTGT TCGCCAGACC
TTCTTCTGGT ACAACGCCAG TATAGGTGAC AACGAAAGTG ACCAGGCCTC AGGTGCCTAC
ATCTTCAGAC CCAACCAACA GAAACCGCTG CCTGTGAGCC GCTGGGCTCA GATCCACCTG
GTGAAGACAC CCTTGGTGCA GGAGGTGCAC CAGAACTTCT CAGCTTGGTG TTCCCAGGTG
GTTCGCCTGT ACCCAGGACA GCGGCACCTG GAGCTAGAGT GGTCGGTGGG GCCGATACCT
GTGGGCGACA CCTGGGGGAA GGAGGTCATC AGCCGTTTTG ACACACCGCT GGAGACAAAG
GGACGCTTCT ACACAGACAG CAATGGCCGG GAGATCCTGG AGAGGAGGCG GGATTATCGA
CCCACCTGGA AACTGAACCA GACGGAGCCC GTGGCAGGAA ACTACTATCC AGTCAACACC
CGGATTTACA TCACGGATGG AAACATGCAG CTGACTGTGC TGACTGACCG CTCCCAGGGG
GGCAGCAGCC TGAGAGATGG CTCGCTGGAG CTCATGGTGC ACCGAAGGCT GCTGAAGGAC
GATGGACGCG GAGTATCGGA GCCACTAATG GAGAACGGGT CGGGGGCGTG GGTGCGAGGG
CGCCACCTGG TGCTGCTGGA CACAGCCCAG GCTGCAGCCG CCGGACACCG GCTCCTGGCG
GAGCAGGAGG TCCTGGCCCC TCAGGTGGTG CTGGCCCCGG GTGGCGGCGC CGCCTACAAT
CTCGGGGCTC CTCCGCGCAC GCAGTTCTCA GGGCTGCGCA GGGACCTGCC GCCCTCGGTG
CACCTGCTCA CGCTGGCCAG CTGGGGCCCC GAAATGGTGC TGCTGCGCTT GGAGCACCAG
TTTGCCGTAG GAGAGGATTC CGGACGTAAC CTGAGCGCCC CCGTTACCTT GAACTTGAGG
GACCTGTTCT CCACCTTCAC CATCACCCGC CTGCAGGAGA CCACGCTGGT GGCCAACCAG
CTCCGCGAGG CAGCCTCCAG GCTCAAGTGG ACAACAAACA CAGGCCCCAC ACCCCACCAA
ACTCCGTACC AGCTGGACCC GGCCAACATC ACGCTGGAAC CCATGGAAAT CCGCACTTTC
CTGGCCTCAG TTCAATGGAA GGAGGTGGAT GGTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16780
Clone ID Related Accession (Same CDS sequence) NM_001173498
Accession Version NM_001173498.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3033bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product lysosomal alpha-mannosidase isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB237901.1, U05572.1, AK291572.1 and BM982008.1. Transcript Variant: This variant (2) lacks a CAG copy at an splice site in the CDS, as compared to variant 1. The resulting isoform (2) lacks an internal Q, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U05572.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGGGCGCCT ACGCGCGGGC TTCGGGGGTC TGCGCTCGCG GCTGCCTGGA CTCAGCAGGC 
CCCTGGACCA TGTCCCGCGC CCTGCGGCCA CCGCTCCCGC CTCTCTGCTT TTTCCTTTTG
TTGCTGGCGG CTGCCGGTGC TCGGGCCGGG GGATACGAGA CATGCCCCAC AGTGCAGCCG
AACATGCTGA ACGTGCACCT GCTGCCTCAC ACACATGATG ACGTGGGCTG GCTCAAAACC
GTGGACCAGT ACTTTTATGG AATCAAGAAT GACATCCAGC ACGCCGGTGT GCAGTACATC
CTGGACTCGG TCATCTCTGC CTTGCTGGCA GATCCCACCC GTCGCTTCAT TTACGTGGAG
ATTGCCTTCT TCTCCCGTTG GTGGCACCAG CAGACAAATG CCACACAGGA AGTCGTGCGA
GACCTTGTGC GCCAGGGGCG CCTGGAGTTC GCCAATGGTG GCTGGGTGAT GAACGATGAG
GCAGCCACCC ACTACGGTGC CATCGTGGAC CAGATGACAC TTGGGCTGCG CTTTCTGGAG
GACACATTTG GCAATGATGG GCGACCCCGT GTGGCCTGGC ACATTGACCC CTTCGGCCAC
TCTCGGGAGC AGGCCTCGCT GTTTGCGCAG ATGGGCTTCG ACGGCTTCTT CTTTGGGCGC
CTTGATTATC AAGATAAGTG GGTACGGATG CAGAAGCTGG AGATGGAGCA GGTGTGGCGG
GCCAGCACCA GCCTGAAGCC CCCGACCGCG GACCTCTTCA CTGGTGTGCT TCCCAATGGT
TACAACCCGC CAAGGAATCT GTGCTGGGAT GTGCTGTGTG TCGATCAGCC GCTGGTGGAG
GACCCTCGCA GCCCCGAGTA CAACGCCAAG GAGCTGGTCG ATTACTTCCT AAATGTGGCC
ACTGCCCAGG GCCGGTATTA CCGCACCAAC CACACTGTGA TGACCATGGG CTCGGACTTC
CAATATGAGA ATGCCAACAT GTGGTTCAAG AACCTTGACA AGCTCATCCG GCTGGTAAAT
GCGCAGGCAA AAGGAAGCAG TGTCCATGTT CTCTACTCCA CCCCCGCTTG TTACCTCTGG
GAGCTGAACA AGGCCAACCT CACCTGGTCA GTGAAACATG ACGACTTCTT CCCTTACGCG
GATGGCCCCC ACCAGTTCTG GACCGGTTAC TTTTCCAGTC GGCCGGCCCT CAAACGCTAC
GAGCGCCTCA GCTACAACTT CCTGCAGGTG TGCAACCAGC TGGAGGCGCT GGTGGGCCTG
GCGGCCAACG TGGGACCCTA TGGCTCCGGA GACAGTGCAC CCCTCAATGA GGCGATGGCT
GTGCTCCAGC ATCACGACGC CGTCAGCGGC ACCTCCCGCC AGCACGTGGC CAACGACTAC
GCGCGCCAGC TTGCGGCAGG CTGGGGGCCT TGCGAGGTTC TTCTGAGCAA CGCGCTGGCG
CGGCTCAGAG GCTTCAAAGA TCACTTCACC TTTTGCCAAC AGCTAAACAT CAGCATCTGC
CCGCTCAGCC AGACGGCGGC GCGCTTCCAG GTCATCGTTT ATAATCCCCT GGGGCGGAAG
GTGAATTGGA TGGTACGGCT GCCGGTCAGC GAAGGCGTTT TCGTTGTGAA GGACCCCAAT
GGCAGGACAG TGCCCAGCGA TGTGGTAATA TTTCCCAGCT CAGACAGCCA GGCGCACCCT
CCGGAGCTGC TGTTCTCAGC CTCACTGCCC GCCCTGGGCT TCAGCACCTA TTCAGTAGCC
CAGGTGCCTC GCTGGAAGCC CCAGGCCCGC GCACCACAGC CCATCCCCAG AAGATCCTGG
TCCCCTGCTT TAACCATCGA AAATGAGCAC ATCCGGGCAA CGTTTGATCC TGACACAGGG
CTGTTGATGG AGATTATGAA CATGAATCAG CAACTCCTGC TGCCTGTTCG CCAGACCTTC
TTCTGGTACA ACGCCAGTAT AGGTGACAAC GAAAGTGACC AGGCCTCAGG TGCCTACATC
TTCAGACCCA ACCAACAGAA ACCGCTGCCT GTGAGCCGCT GGGCTCAGAT CCACCTGGTG
AAGACACCCT TGGTGCAGGA GGTGCACCAG AACTTCTCAG CTTGGTGTTC CCAGGTGGTT
CGCCTGTACC CAGGACAGCG GCACCTGGAG CTAGAGTGGT CGGTGGGGCC GATACCTGTG
GGCGACACCT GGGGGAAGGA GGTCATCAGC CGTTTTGACA CACCGCTGGA GACAAAGGGA
CGCTTCTACA CAGACAGCAA TGGCCGGGAG ATCCTGGAGA GGAGGCGGGA TTATCGACCC
ACCTGGAAAC TGAACCAGAC GGAGCCCGTG GCAGGAAACT ACTATCCAGT CAACACCCGG
ATTTACATCA CGGATGGAAA CATGCAGCTG ACTGTGCTGA CTGACCGCTC CCAGGGGGGC
AGCAGCCTGA GAGATGGCTC GCTGGAGCTC ATGGTGCACC GAAGGCTGCT GAAGGACGAT
GGACGCGGAG TATCGGAGCC ACTAATGGAG AACGGGTCGG GGGCGTGGGT GCGAGGGCGC
CACCTGGTGC TGCTGGACAC AGCCCAGGCT GCAGCCGCCG GACACCGGCT CCTGGCGGAG
CAGGAGGTCC TGGCCCCTCA GGTGGTGCTG GCCCCGGGTG GCGGCGCCGC CTACAATCTC
GGGGCTCCTC CGCGCACGCA GTTCTCAGGG CTGCGCAGGG ACCTGCCGCC CTCGGTGCAC
CTGCTCACGC TGGCCAGCTG GGGCCCCGAA ATGGTGCTGC TGCGCTTGGA GCACCAGTTT
GCCGTAGGAG AGGATTCCGG ACGTAACCTG AGCGCCCCCG TTACCTTGAA CTTGAGGGAC
CTGTTCTCCA CCTTCACCAT CACCCGCCTG CAGGAGACCA CGCTGGTGGC CAACCAGCTC
CGCGAGGCAG CCTCCAGGCT CAAGTGGACA ACAAACACAG GCCCCACACC CCACCAAACT
CCGTACCAGC TGGACCCGGC CAACATCACG CTGGAACCCA TGGAAATCCG CACTTTCCTG
GCCTCAGTTC AATGGAAGGA GGTGGATGGT TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001166969.1
CDS77..3109
Misc Feature(1)71..73(+)
Misc Feature(2)263..3073(+)
Misc Feature(3)263..1099(+)
Misc Feature(4)290..1033(+)
Misc Feature(5)296..805(+)
Misc Feature(6)473..475(+)
Misc Feature(7)662..1033(+)
Misc Feature(8)1232..1468(+)
Misc Feature(9)1601..3073(+)
Exon (1)1..235
Gene:MAN2B1
Gene Synonym:
Exon (2)236..338
Gene:MAN2B1
Gene Synonym:
Exon (3)339..512
Gene:MAN2B1
Gene Synonym:
Exon (4)513..706
Gene:MAN2B1
Gene Synonym:
Exon (5)707..839
Gene:MAN2B1
Gene Synonym:
Exon (6)840..985
Gene:MAN2B1
Gene Synonym:
Exon (7)986..1102
Gene:MAN2B1
Gene Synonym:
Exon (8)1103..1182
Gene:MAN2B1
Gene Synonym:
Exon (9)1183..1303
Gene:MAN2B1
Gene Synonym:
Exon (10)1304..1382
Gene:MAN2B1
Gene Synonym:
Exon (11)1383..1492
Gene:MAN2B1
Gene Synonym:
Exon (12)1493..1600
Gene:MAN2B1
Gene Synonym:
Exon (13)1601..1717
Gene:MAN2B1
Gene Synonym:
Exon (14)1718..1903
Gene:MAN2B1
Gene Synonym:
Exon (15)1904..2001
Gene:MAN2B1
Gene Synonym:
Exon (16)2002..2119
Gene:MAN2B1
Gene Synonym:
Exon (17)2120..2238
Gene:MAN2B1
Gene Synonym:
Exon (18)2239..2340
Gene:MAN2B1
Gene Synonym:
Exon (19)2341..2428
Gene:MAN2B1
Gene Synonym:
Exon (20)2429..2509
Gene:MAN2B1
Gene Synonym:
Exon (21)2510..2737
Gene:MAN2B1
Gene Synonym:
Exon (22)2738..2893
Gene:MAN2B1
Gene Synonym:
Exon (23)2894..2996
Gene:MAN2B1
Gene Synonym:
Exon (24)2997..3221
Gene:MAN2B1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001173498
Organism Homo sapiens (human)
Definition Homo sapiens mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001173498

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGGGCGCCT ACGCGCGGGC TTCGGGGGTC TGCGCTCGCG GCTGCCTGGA CTCAGCAGGC 
CCCTGGACCA TGTCCCGCGC CCTGCGGCCA CCGCTCCCGC CTCTCTGCTT TTTCCTTTTG
TTGCTGGCGG CTGCCGGTGC TCGGGCCGGG GGATACGAGA CATGCCCCAC AGTGCAGCCG
AACATGCTGA ACGTGCACCT GCTGCCTCAC ACACATGATG ACGTGGGCTG GCTCAAAACC
GTGGACCAGT ACTTTTATGG AATCAAGAAT GACATCCAGC ACGCCGGTGT GCAGTACATC
CTGGACTCGG TCATCTCTGC CTTGCTGGCA GATCCCACCC GTCGCTTCAT TTACGTGGAG
ATTGCCTTCT TCTCCCGTTG GTGGCACCAG CAGACAAATG CCACACAGGA AGTCGTGCGA
GACCTTGTGC GCCAGGGGCG CCTGGAGTTC GCCAATGGTG GCTGGGTGAT GAACGATGAG
GCAGCCACCC ACTACGGTGC CATCGTGGAC CAGATGACAC TTGGGCTGCG CTTTCTGGAG
GACACATTTG GCAATGATGG GCGACCCCGT GTGGCCTGGC ACATTGACCC CTTCGGCCAC
TCTCGGGAGC AGGCCTCGCT GTTTGCGCAG ATGGGCTTCG ACGGCTTCTT CTTTGGGCGC
CTTGATTATC AAGATAAGTG GGTACGGATG CAGAAGCTGG AGATGGAGCA GGTGTGGCGG
GCCAGCACCA GCCTGAAGCC CCCGACCGCG GACCTCTTCA CTGGTGTGCT TCCCAATGGT
TACAACCCGC CAAGGAATCT GTGCTGGGAT GTGCTGTGTG TCGATCAGCC GCTGGTGGAG
GACCCTCGCA GCCCCGAGTA CAACGCCAAG GAGCTGGTCG ATTACTTCCT AAATGTGGCC
ACTGCCCAGG GCCGGTATTA CCGCACCAAC CACACTGTGA TGACCATGGG CTCGGACTTC
CAATATGAGA ATGCCAACAT GTGGTTCAAG AACCTTGACA AGCTCATCCG GCTGGTAAAT
GCGCAGGCAA AAGGAAGCAG TGTCCATGTT CTCTACTCCA CCCCCGCTTG TTACCTCTGG
GAGCTGAACA AGGCCAACCT CACCTGGTCA GTGAAACATG ACGACTTCTT CCCTTACGCG
GATGGCCCCC ACCAGTTCTG GACCGGTTAC TTTTCCAGTC GGCCGGCCCT CAAACGCTAC
GAGCGCCTCA GCTACAACTT CCTGCAGGTG TGCAACCAGC TGGAGGCGCT GGTGGGCCTG
GCGGCCAACG TGGGACCCTA TGGCTCCGGA GACAGTGCAC CCCTCAATGA GGCGATGGCT
GTGCTCCAGC ATCACGACGC CGTCAGCGGC ACCTCCCGCC AGCACGTGGC CAACGACTAC
GCGCGCCAGC TTGCGGCAGG CTGGGGGCCT TGCGAGGTTC TTCTGAGCAA CGCGCTGGCG
CGGCTCAGAG GCTTCAAAGA TCACTTCACC TTTTGCCAAC AGCTAAACAT CAGCATCTGC
CCGCTCAGCC AGACGGCGGC GCGCTTCCAG GTCATCGTTT ATAATCCCCT GGGGCGGAAG
GTGAATTGGA TGGTACGGCT GCCGGTCAGC GAAGGCGTTT TCGTTGTGAA GGACCCCAAT
GGCAGGACAG TGCCCAGCGA TGTGGTAATA TTTCCCAGCT CAGACAGCCA GGCGCACCCT
CCGGAGCTGC TGTTCTCAGC CTCACTGCCC GCCCTGGGCT TCAGCACCTA TTCAGTAGCC
CAGGTGCCTC GCTGGAAGCC CCAGGCCCGC GCACCACAGC CCATCCCCAG AAGATCCTGG
TCCCCTGCTT TAACCATCGA AAATGAGCAC ATCCGGGCAA CGTTTGATCC TGACACAGGG
CTGTTGATGG AGATTATGAA CATGAATCAG CAACTCCTGC TGCCTGTTCG CCAGACCTTC
TTCTGGTACA ACGCCAGTAT AGGTGACAAC GAAAGTGACC AGGCCTCAGG TGCCTACATC
TTCAGACCCA ACCAACAGAA ACCGCTGCCT GTGAGCCGCT GGGCTCAGAT CCACCTGGTG
AAGACACCCT TGGTGCAGGA GGTGCACCAG AACTTCTCAG CTTGGTGTTC CCAGGTGGTT
CGCCTGTACC CAGGACAGCG GCACCTGGAG CTAGAGTGGT CGGTGGGGCC GATACCTGTG
GGCGACACCT GGGGGAAGGA GGTCATCAGC CGTTTTGACA CACCGCTGGA GACAAAGGGA
CGCTTCTACA CAGACAGCAA TGGCCGGGAG ATCCTGGAGA GGAGGCGGGA TTATCGACCC
ACCTGGAAAC TGAACCAGAC GGAGCCCGTG GCAGGAAACT ACTATCCAGT CAACACCCGG
ATTTACATCA CGGATGGAAA CATGCAGCTG ACTGTGCTGA CTGACCGCTC CCAGGGGGGC
AGCAGCCTGA GAGATGGCTC GCTGGAGCTC ATGGTGCACC GAAGGCTGCT GAAGGACGAT
GGACGCGGAG TATCGGAGCC ACTAATGGAG AACGGGTCGG GGGCGTGGGT GCGAGGGCGC
CACCTGGTGC TGCTGGACAC AGCCCAGGCT GCAGCCGCCG GACACCGGCT CCTGGCGGAG
CAGGAGGTCC TGGCCCCTCA GGTGGTGCTG GCCCCGGGTG GCGGCGCCGC CTACAATCTC
GGGGCTCCTC CGCGCACGCA GTTCTCAGGG CTGCGCAGGG ACCTGCCGCC CTCGGTGCAC
CTGCTCACGC TGGCCAGCTG GGGCCCCGAA ATGGTGCTGC TGCGCTTGGA GCACCAGTTT
GCCGTAGGAG AGGATTCCGG ACGTAACCTG AGCGCCCCCG TTACCTTGAA CTTGAGGGAC
CTGTTCTCCA CCTTCACCAT CACCCGCCTG CAGGAGACCA CGCTGGTGGC CAACCAGCTC
CGCGAGGCAG CCTCCAGGCT CAAGTGGACA ACAAACACAG GCCCCACACC CCACCAAACT
CCGTACCAGC TGGACCCGGC CAACATCACG CTGGAACCCA TGGAAATCCG CACTTTCCTG
GCCTCAGTTC AATGGAAGGA GGTGGATGGT TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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