×

MIF cDNA ORF clone, Homo sapiens (human)

Gene Symbol MIF
Entrez Gene ID 4282
Full Name macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Synonyms GIF, GLIF, MMIF
General protein information
Preferred Names
macrophage migration inhibitory factor
Names
macrophage migration inhibitory factor
L-dopachrome isomerase
L-dopachrome tautomerase
phenylpyruvate tautomerase
NP_002406.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

22

22q11.23

Summary This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

153620

Disorder Html: {Rheumatoid arthritis, systemic juvenile, susceptibility to},





The following MIF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu25891 NM_002415 Homo sapiens macrophage migration inhibitory factor (glycosylation-inhibiting factor) (MIF), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.30
$99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu25891
Accession Version NM_002415.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 348bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product macrophage migration inhibitory factor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L19686.1. This sequence is a reference standard in the RefSeqGene project. Summary: This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU599051.1, BC022414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGCCGATGT TCATCGTAAA CACCAACGTG CCCCGCGCCT CCGTGCCGGA CGGGTTCCTC 
TCCGAGCTCA CCCAGCAGCT GGCGCAGGCC ACCGGCAAGC CCCCCCAGTA CATCGCGGTG
CACGTGGTCC CGGACCAGCT CATGGCCTTC GGCGGCTCCA GCGAGCCGTG CGCGCTCTGC
AGCCTGCACA GCATCGGCAA GATCGGCGGC GCGCAGAACC GCTCCTACAG CAAGCTGCTG
TGCGGCCTGC TGGCCGAGCG CCTGCGCATC AGCCCGGACA GGGTCTACAT CAACTATTAC
GACATGAACG CGGCCAATGT GGGCTGGAAC AACTCCACCT TCGCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_002406.1
CDS98..445
Misc Feature(1)101..442(+)
Misc Feature(2)206..208(+)
Misc Feature(3)329..331(+)
Exon (1)1..205
Gene:MIF
Gene Synonym:
Exon (2)206..378
Gene:MIF
Gene Synonym:
Exon (3)379..561
Gene:MIF
Gene Synonym:
Translation
Position Chain Variation Link
16 16 a, g dbSNP:34914548
24 24 a, t dbSNP:112568463
27 27 a, c dbSNP:17004038
29 29 c, t dbSNP:748288791
36 36 a, c dbSNP:201326009
44 44 c, g dbSNP:201939799
45 45 c, g dbSNP:200266174
48 48 c, t dbSNP:767714786
51 51 c, g, t dbSNP:750947661
53 53 c, t dbSNP:780503494
57 57 c, t dbSNP:200992026
58 58 c, g dbSNP:754446348
61 61 c, t dbSNP:778637083
63 63 -, gcctctgc dbSNP:776908147
64 64 a, g, t dbSNP:202123293
66 66 c, g dbSNP:771926589
67 67 c, t dbSNP:777672045
68 68 c, t dbSNP:568954662
73 73 c, t dbSNP:771043681
74 74 a, g, t dbSNP:149423910
76 76 g, t dbSNP:201359524
78 78 c, t dbSNP:768549347
80 80 c, g, t dbSNP:199582661
81 81 c, t dbSNP:200428013
83 83 g, t dbSNP:1803649
87 87 c, g dbSNP:767895810
90 90 c, t dbSNP:201249175
93 93 c, t dbSNP:750822928
94 94 c, t dbSNP:761003099
95 95 a, g dbSNP:199537781
102 102 a, c, g dbSNP:200500959
103 103 a, g dbSNP:754279861
108 108 a, t dbSNP:754533270
112 112 c, g, t dbSNP:201465617
115 115 a, c dbSNP:756999331
121 121 c, t dbSNP:758028444
122 122 a, g dbSNP:777547459
124 124 c, t dbSNP:554255392
128 128 -, ca dbSNP:770070382
131 131 c, t dbSNP:748071324
134 134 a, g dbSNP:757112516
136 136 c, t dbSNP:781277372
140 140 a, g dbSNP:199714772
142 142 g, t dbSNP:11548057
144 144 a, c, g, t dbSNP:201862457
151 151 c, g dbSNP:748223342
161 161 a, g dbSNP:772327844
164 164 c, t dbSNP:533563568
167 167 a, t dbSNP:200995600
172 172 a, g dbSNP:11548058
178 178 -, a dbSNP:775366540
179 179 -, ctga dbSNP:763051070
180 180 c, t dbSNP:766674911
181 181 a, g dbSNP:777221051
184 184 a, g dbSNP:759925267
189 189 c, t dbSNP:765744422
191 191 a, g dbSNP:752199048
196 196 -, c dbSNP:767392036
197 197 a, c, g dbSNP:201631604
199 199 a, c dbSNP:763906852
201 201 c, t dbSNP:199980863
206 206 c, t dbSNP:530185151
214 214 a, g dbSNP:753720782
218 218 c, t dbSNP:202066662
219 219 a, c dbSNP:200005486
220 220 c, g dbSNP:777795012
227 227 c, g dbSNP:747064040
228 228 a, g dbSNP:11548059
239 239 a, g, t dbSNP:372575900
240 240 c, t dbSNP:746286559
243 243 c, t dbSNP:770131530
244 244 c, g dbSNP:11548055
248 248 c, g dbSNP:775873351
251 251 g, t dbSNP:560265113
258 258 g, t dbSNP:763503862
259 259 c, g dbSNP:768313072
264 264 c, g dbSNP:774043537
269 269 a, g dbSNP:761381011
270 270 c, t dbSNP:767129873
272 272 c, t dbSNP:1049829
280 280 c, t dbSNP:750083190
287 287 a, g dbSNP:201307782
292 292 c, g dbSNP:532485317
294 294 a, g dbSNP:766152109
295 295 c, t dbSNP:201952555
300 300 c, t dbSNP:11548056
301 301 a, c dbSNP:754953765
304 304 c, t dbSNP:778803886
308 308 a, c, g dbSNP:200394994
313 313 c, g dbSNP:757354234
321 321 c, t dbSNP:182012324
322 322 c, t dbSNP:746043539
335 335 c, t dbSNP:770074228
340 340 c, t dbSNP:201837402
346 346 a, g dbSNP:200403178
348 348 g, t dbSNP:780425386
352 352 c, g dbSNP:34060481
353 353 a, g dbSNP:201060788
355 355 a, g dbSNP:201099096
357 357 a, g dbSNP:376184469
359 359 c, t dbSNP:34733430
371 371 c, t dbSNP:201742529
372 372 c, g dbSNP:199774339
373 373 a, g dbSNP:771540178
386 386 a, g dbSNP:201792625
387 387 c, t dbSNP:372052137
393 393 a, g dbSNP:200286358
394 394 c, t dbSNP:201227875
398 398 c, g dbSNP:746482585
404 404 a, t dbSNP:770679354
406 406 c, t dbSNP:776261679
410 410 a, g dbSNP:575017934
414 414 a, g dbSNP:1803976
418 418 a, g dbSNP:775430739
435 435 c, g, t dbSNP:139210892
436 436 c, g dbSNP:201956930
441 441 c, t dbSNP:200329745
450 450 c, t dbSNP:201417893
455 455 a, g dbSNP:35565661
456 456 a, g dbSNP:200824910
462 462 c, g dbSNP:201241742
468 468 c, t dbSNP:1050605
477 477 c, t dbSNP:766770539
479 479 c, t dbSNP:754044308
492 492 c, t dbSNP:546132934
495 495 c, t dbSNP:376078573
496 496 a, g, t dbSNP:199587874
497 497 a, c, t dbSNP:200721210
500 500 a, g, t dbSNP:36065127
501 501 c, g dbSNP:548015679
506 506 g, t dbSNP:770361893
508 508 c, t dbSNP:3191127
509 509 c, t dbSNP:200174784
510 510 a, g dbSNP:200716985
512 512 a, c, g dbSNP:201740473
520 520 -, c dbSNP:778398454
520 520 a, t dbSNP:200078978
526 526 a, c dbSNP:762632802
528 528 c, t dbSNP:200980127
530 530 g, t dbSNP:774146748
531 531 c, t dbSNP:3191218
536 536 a, g dbSNP:760810367
542 542 a, c dbSNP:201308167
549 549 c, t dbSNP:754132407
555 555 a, c dbSNP:199989428
559 559 a, g dbSNP:201104479
561 561 a, t dbSNP:112102057

Target ORF information:

RefSeq Version NM_002415
Organism Homo sapiens (human)
Definition Homo sapiens macrophage migration inhibitory factor (glycosylation-inhibiting factor) (MIF), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002415

ORF Insert Sequence:

1
61
121
181
241
301
ATGCCGATGT TCATCGTAAA CACCAACGTG CCCCGCGCCT CCGTGCCGGA CGGGTTCCTC 
TCCGAGCTCA CCCAGCAGCT GGCGCAGGCC ACCGGCAAGC CCCCCCAGTA CATCGCGGTG
CACGTGGTCC CGGACCAGCT CATGGCCTTC GGCGGCTCCA GCGAGCCGTG CGCGCTCTGC
AGCCTGCACA GCATCGGCAA GATCGGCGGC GCGCAGAACC GCTCCTACAG CAAGCTGCTG
TGCGGCCTGC TGGCCGAGCG CCTGCGCATC AGCCCGGACA GGGTCTACAT CAACTATTAC
GACATGAACG CGGCCAATGT GGGCTGGAAC AACTCCACCT TCGCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
*
*
*
*