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MLH1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MLH1
Entrez Gene ID 4292
Full Name mutL homolog 1
Synonyms COCA2, FCC2, HNPCC, HNPCC2, hMLH1
General protein information
Preferred Names
DNA mismatch repair protein Mlh1
Names
DNA mismatch repair protein Mlh1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

3

3p21.3

Summary This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.[provided by RefSeq, Nov 2009]. lac of sum
Disorder MIM:

120436

Disorder Html: Colorectal cancer, hereditary nonpolyposis, type 2, 609310 (3);

mRNA and Protein(s)

mRNA Protein Name
XM_005265161 XP_005265218 DNA mismatch repair protein Mlh1 isoform X2
XM_005265163 XP_005265220 DNA mismatch repair protein Mlh1 isoform X1
XM_005265164 XP_005265221 DNA mismatch repair protein Mlh1 isoform X1
XM_005265166 XP_005265223 DNA mismatch repair protein Mlh1 isoform X3
XM_011533727 XP_011532029 DNA mismatch repair protein Mlh1 isoform X4
NM_001258274 NP_001245203 DNA mismatch repair protein Mlh1 isoform 3
NM_001258271 NP_001245200 DNA mismatch repair protein Mlh1 isoform 4
NM_001258273 NP_001245202 DNA mismatch repair protein Mlh1 isoform 3
NM_000249 NP_000240 DNA mismatch repair protein Mlh1 isoform 1
NM_001167617 NP_001161089 DNA mismatch repair protein Mlh1 isoform 2
NM_001167618 NP_001161090 DNA mismatch repair protein Mlh1 isoform 3
NM_001167619 NP_001161091 DNA mismatch repair protein Mlh1 isoform 3



Homo sapiens (human) MLH1 NP_000240.1
Pan troglodytes (chimpanzee) MLH1 XP_001170433.1
Canis lupus familiaris (dog) MLH1 XP_534219.2
Bos taurus (cattle) MLH1 NP_001069462.2
Mus musculus (house mouse) Mlh1 NP_081086.2
Rattus norvegicus (Norway rat) Mlh1 NP_112315.1
Gallus gallus (chicken) MLH1 XP_418828.1
Danio rerio (zebrafish) mlh1 NP_956953.1
Drosophila melanogaster (fruit fly) Mlh1 NP_477022.1
Arabidopsis thaliana (thale cress) MLH1 NP_567345.2
Xenopus (Silurana) tropicalis (western clawed frog) mlh1 XP_002933455.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MLH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MLH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu37255 XM_005265161 PREDICTED: Homo sapiens mutL homolog 1 (MLH1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu20930
XM_005265163 PREDICTED: Homo sapiens mutL homolog 1 (MLH1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
OHu20930
XM_005265164 PREDICTED: Homo sapiens mutL homolog 1 (MLH1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
OHu37256 XM_005265166 PREDICTED: Homo sapiens mutL homolog 1 (MLH1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu58984 XM_011533727 PREDICTED: Homo sapiens mutL homolog 1 (MLH1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu20930
NM_001258274 Homo sapiens mutL homolog 1 (MLH1), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
OHu27476 NM_001258271 Homo sapiens mutL homolog 1 (MLH1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu20930
NM_001258273 Homo sapiens mutL homolog 1 (MLH1), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
OHu26603
NM_000249 Homo sapiens mutL homolog 1 (MLH1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
OHu27066 NM_001167617 Homo sapiens mutL homolog 1 (MLH1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu20930
NM_001167618 Homo sapiens mutL homolog 1 (MLH1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
OHu20930
NM_001167619 Homo sapiens mutL homolog 1 (MLH1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu37255
Clone ID Related Accession (Same CDS sequence) XM_005265161
Accession Version XM_005265161.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2064bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA mismatch repair protein Mlh1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGTCGTTCG TGGCAGGGGT TATTCGGCGG CTGGACGAGA CAGTGGTGAA CCGCATCGCG 
GCGGGGGAAG TTATCCAGCG GCCAGCTAAT GCTATCAAAG AGATGATTGA GAACTGTTTA
GATGCAAAAT CCACAAGTAT TCAAGTGATT GTTAAAGAGG GAGGCCTGAA GTTGATTCAG
ATCCAAGACA ATGGCACCGG GATCAGGAAA GAAGATCTGG ATATTGTATG TGAAAGGTTC
ACTACTAGTA AACTGCAGTC CTTTGAGGAT TTAGCCAGTA TTTCTACCTA TGGCTTTCGA
GGTGAGGCTT TGGCCAGCAT AAGCCATGTG GCTCATGTTA CTATTACAAC GAAAACAGCT
GATGGAAAGT GTGCATACAG AGCAAGTTAC TCAGATGGAA AACTGAAAGC CCCTCCTAAA
CCATGTGCTG GCAATCAAGG GACCCAGATC ACGGTGGAGG ACCTTTTTTA CAACATAGCC
ACGAGGAGAA AAGCTTTAAA AAATCCAAGT GAAGAATATG GGAAAATTTT GGAAGTTGTT
GGCAGGTATT CAGTACACAA TGCAGGCATT AGTTTCTCAG TTAAAAAACA AGGAGAGACA
GTAGCTGATG TTAGGACACT ACCCAATGCC TCAACCGTGG ACAATATTCG CTCCATCTTT
GGAAATGCTG TTAGTCGTTT AGAAATCAGT CCCCAGAATG TGGATGTTAA TGTGCACCCC
ACAAAGCATG AAGTTCACTT CCTGCACGAG GAGAGCATCC TGGAGCGGGT GCAGCAGCAC
ATCGAGAGCA AGCTCCTGGG CTCCAATTCC TCCAGGATGT ACTTCACCCA GACTTTGCTA
CCAGGACTTG CTGGCCCCTC TGGGGAGATG GTTAAATCCA CAACAAGTCT GACCTCGTCT
TCTACTTCTG GAAGTAGTGA TAAGGTCTAT GCCCACCAGA TGGTTCGTAC AGATTCCCGG
GAACAGAAGC TTGATGCATT TCTGCAGCCT CTGAGCAAAC CCCTGTCCAG TCAGCCCCAG
GCCATTGTCA CAGAGGATAA GACAGATATT TCTAGTGGCA GGGCTAGGCA GCAAGATGAG
GAGATGCTTG AACTCCCAGC CCCTGCTGAA GTGGCTGCCA AAAATCAGAG CTTGGAGGGG
GATACAACAA AGGGGACTTC AGAAATGTCA GAGAAGAGAG GACCTACTTC CAGCAACCCC
AGAAAGAGAC ATCGGGAAGA TTCTGATGTG GAAATGGTGG AAGATGATTC CCGAAAGGAA
ATGACTGCAG CTTGTACCCC CCGGAGAAGG ATCATTAACC TCACTAGTGT TTTGAGTCTC
CAGGAAGAAA TTAATGAGCA GGGACATGAG GTTCTCCGGG AGATGTTGCA TAACCACTCC
TTCGTGGGCT GTGTGAATCC TCAGTGGGCC TTGGCACAGC ATCAAACCAA GTTATACCTT
CTCAACACCA CCAAGCTTAG TGAAGAACTG TTCTACCAGA TACTCATTTA TGATTTTGCC
AATTTTGGTG TTCTCAGGTT ATCGGAGCCA GCACCGCTCT TTGACCTTGC CATGCTTGCC
TTAGATAGTC CAGAGAGTGG CTGGACAGAG GAAGATGGTC CCAAAGAAGG ACTTGCTGAA
TACATTGTTG AGTTTCTGAA GAAGAAGGCT GAGATGCTTG CAGACTATTT CTCTTTGGAA
ATTGATGAGG AAGGGAACCT GATTGGATTA CCCCTTCTGA TTGACAACTA TGTGCCCCCT
TTGGAGGGAC TGCCTATCTT CATTCTTCGA CTAGCCACTG AGGTGAATTG GGACGAAGAA
AAGGAATGTT TTGAAAGCCT CAGTAAAGAA TGCGCTATGT TCTATTCCAT CCGGAAGCAG
TACATATCTG AGGAGTCGAC CCTCTCAGGC CAGCAGAGTG AAGTGCCTGG CTCCATTCCA
AACTCCTGGA AGTGGACTGT GGAACACATT GTCTATAAAG CCTTGCGCTC ACACATTCTG
CCTCCTAAAC ATTTCACAGA AGATGGAAAT ATCCTGCAGC TTGCTAACCT GCCTGATCTA
TACAAAGTCT TTGAGAGGTG TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265218.1
CDS89..2152
Misc Feature(1)104..826(+)
Misc Feature(2)179..454(+)
Misc Feature(3)188..454(+)
Misc Feature(4)200..202(+)
Misc Feature(5)281..397(+)
Misc Feature(6)767..886(+)
Misc Feature(7)812..814(+)
Translation

Target ORF information:

RefSeq Version XM_005265161
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mutL homolog 1 (MLH1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005265161

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGTCGTTCG TGGCAGGGGT TATTCGGCGG CTGGACGAGA CAGTGGTGAA CCGCATCGCG 
GCGGGGGAAG TTATCCAGCG GCCAGCTAAT GCTATCAAAG AGATGATTGA GAACTGTTTA
GATGCAAAAT CCACAAGTAT TCAAGTGATT GTTAAAGAGG GAGGCCTGAA GTTGATTCAG
ATCCAAGACA ATGGCACCGG GATCAGGAAA GAAGATCTGG ATATTGTATG TGAAAGGTTC
ACTACTAGTA AACTGCAGTC CTTTGAGGAT TTAGCCAGTA TTTCTACCTA TGGCTTTCGA
GGTGAGGCTT TGGCCAGCAT AAGCCATGTG GCTCATGTTA CTATTACAAC GAAAACAGCT
GATGGAAAGT GTGCATACAG AGCAAGTTAC TCAGATGGAA AACTGAAAGC CCCTCCTAAA
CCATGTGCTG GCAATCAAGG GACCCAGATC ACGGTGGAGG ACCTTTTTTA CAACATAGCC
ACGAGGAGAA AAGCTTTAAA AAATCCAAGT GAAGAATATG GGAAAATTTT GGAAGTTGTT
GGCAGGTATT CAGTACACAA TGCAGGCATT AGTTTCTCAG TTAAAAAACA AGGAGAGACA
GTAGCTGATG TTAGGACACT ACCCAATGCC TCAACCGTGG ACAATATTCG CTCCATCTTT
GGAAATGCTG TTAGTCGTTT AGAAATCAGT CCCCAGAATG TGGATGTTAA TGTGCACCCC
ACAAAGCATG AAGTTCACTT CCTGCACGAG GAGAGCATCC TGGAGCGGGT GCAGCAGCAC
ATCGAGAGCA AGCTCCTGGG CTCCAATTCC TCCAGGATGT ACTTCACCCA GACTTTGCTA
CCAGGACTTG CTGGCCCCTC TGGGGAGATG GTTAAATCCA CAACAAGTCT GACCTCGTCT
TCTACTTCTG GAAGTAGTGA TAAGGTCTAT GCCCACCAGA TGGTTCGTAC AGATTCCCGG
GAACAGAAGC TTGATGCATT TCTGCAGCCT CTGAGCAAAC CCCTGTCCAG TCAGCCCCAG
GCCATTGTCA CAGAGGATAA GACAGATATT TCTAGTGGCA GGGCTAGGCA GCAAGATGAG
GAGATGCTTG AACTCCCAGC CCCTGCTGAA GTGGCTGCCA AAAATCAGAG CTTGGAGGGG
GATACAACAA AGGGGACTTC AGAAATGTCA GAGAAGAGAG GACCTACTTC CAGCAACCCC
AGAAAGAGAC ATCGGGAAGA TTCTGATGTG GAAATGGTGG AAGATGATTC CCGAAAGGAA
ATGACTGCAG CTTGTACCCC CCGGAGAAGG ATCATTAACC TCACTAGTGT TTTGAGTCTC
CAGGAAGAAA TTAATGAGCA GGGACATGAG GTTCTCCGGG AGATGTTGCA TAACCACTCC
TTCGTGGGCT GTGTGAATCC TCAGTGGGCC TTGGCACAGC ATCAAACCAA GTTATACCTT
CTCAACACCA CCAAGCTTAG TGAAGAACTG TTCTACCAGA TACTCATTTA TGATTTTGCC
AATTTTGGTG TTCTCAGGTT ATCGGAGCCA GCACCGCTCT TTGACCTTGC CATGCTTGCC
TTAGATAGTC CAGAGAGTGG CTGGACAGAG GAAGATGGTC CCAAAGAAGG ACTTGCTGAA
TACATTGTTG AGTTTCTGAA GAAGAAGGCT GAGATGCTTG CAGACTATTT CTCTTTGGAA
ATTGATGAGG AAGGGAACCT GATTGGATTA CCCCTTCTGA TTGACAACTA TGTGCCCCCT
TTGGAGGGAC TGCCTATCTT CATTCTTCGA CTAGCCACTG AGGTGAATTG GGACGAAGAA
AAGGAATGTT TTGAAAGCCT CAGTAAAGAA TGCGCTATGT TCTATTCCAT CCGGAAGCAG
TACATATCTG AGGAGTCGAC CCTCTCAGGC CAGCAGAGTG AAGTGCCTGG CTCCATTCCA
AACTCCTGGA AGTGGACTGT GGAACACATT GTCTATAAAG CCTTGCGCTC ACACATTCTG
CCTCCTAAAC ATTTCACAGA AGATGGAAAT ATCCTGCAGC TTGCTAACCT GCCTGATCTA
TACAAAGTCT TTGAGAGGTG TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20930
Clone ID Related Accession (Same CDS sequence) NM_001167618 , NM_001167619 , NM_001258273 , NM_001258274 , XM_005265163 , XM_005265164
Accession Version XM_005265163.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1548bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA mismatch repair protein Mlh1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATGGTT ACATATCCAA TGCAAACTAC TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA ATCAACTTCC TTGAGAAAAG CCATAGAAAC AGTGTATGCA
GCCTATTTGC CCAAAAACAC ACACCCATTC CTGTACCTCA GTTTAGAAAT CAGTCCCCAG
AATGTGGATG TTAATGTGCA CCCCACAAAG CATGAAGTTC ACTTCCTGCA CGAGGAGAGC
ATCCTGGAGC GGGTGCAGCA GCACATCGAG AGCAAGCTCC TGGGCTCCAA TTCCTCCAGG
ATGTACTTCA CCCAGACTTT GCTACCAGGA CTTGCTGGCC CCTCTGGGGA GATGGTTAAA
TCCACAACAA GTCTGACCTC GTCTTCTACT TCTGGAAGTA GTGATAAGGT CTATGCCCAC
CAGATGGTTC GTACAGATTC CCGGGAACAG AAGCTTGATG CATTTCTGCA GCCTCTGAGC
AAACCCCTGT CCAGTCAGCC CCAGGCCATT GTCACAGAGG ATAAGACAGA TATTTCTAGT
GGCAGGGCTA GGCAGCAAGA TGAGGAGATG CTTGAACTCC CAGCCCCTGC TGAAGTGGCT
GCCAAAAATC AGAGCTTGGA GGGGGATACA ACAAAGGGGA CTTCAGAAAT GTCAGAGAAG
AGAGGACCTA CTTCCAGCAA CCCCAGAAAG AGACATCGGG AAGATTCTGA TGTGGAAATG
GTGGAAGATG ATTCCCGAAA GGAAATGACT GCAGCTTGTA CCCCCCGGAG AAGGATCATT
AACCTCACTA GTGTTTTGAG TCTCCAGGAA GAAATTAATG AGCAGGGACA TGAGGTTCTC
CGGGAGATGT TGCATAACCA CTCCTTCGTG GGCTGTGTGA ATCCTCAGTG GGCCTTGGCA
CAGCATCAAA CCAAGTTATA CCTTCTCAAC ACCACCAAGC TTAGTGAAGA ACTGTTCTAC
CAGATACTCA TTTATGATTT TGCCAATTTT GGTGTTCTCA GGTTATCGGA GCCAGCACCG
CTCTTTGACC TTGCCATGCT TGCCTTAGAT AGTCCAGAGA GTGGCTGGAC AGAGGAAGAT
GGTCCCAAAG AAGGACTTGC TGAATACATT GTTGAGTTTC TGAAGAAGAA GGCTGAGATG
CTTGCAGACT ATTTCTCTTT GGAAATTGAT GAGGAAGGGA ACCTGATTGG ATTACCCCTT
CTGATTGACA ACTATGTGCC CCCTTTGGAG GGACTGCCTA TCTTCATTCT TCGACTAGCC
ACTGAGGTGA ATTGGGACGA AGAAAAGGAA TGTTTTGAAA GCCTCAGTAA AGAATGCGCT
ATGTTCTATT CCATCCGGAA GCAGTACATA TCTGAGGAGT CGACCCTCTC AGGCCAGCAG
AGTGAAGTGC CTGGCTCCAT TCCAAACTCC TGGAAGTGGA CTGTGGAACA CATTGTCTAT
AAAGCCTTGC GCTCACACAT TCTGCCTCCT AAACATTTCA CAGAAGATGG AAATATCCTG
CAGCTTGCTA ACCTGCCTGA TCTATACAAA GTCTTTGAGA GGTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265220.1
CDS722..2269
Misc Feature(1)722..1003(+)
Misc Feature(2)725..2059(+)
Misc Feature(3)929..931(+)
Translation

Target ORF information:

RefSeq Version XM_005265163
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mutL homolog 1 (MLH1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005265163

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATGGTT ACATATCCAA TGCAAACTAC TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA ATCAACTTCC TTGAGAAAAG CCATAGAAAC AGTGTATGCA
GCCTATTTGC CCAAAAACAC ACACCCATTC CTGTACCTCA GTTTAGAAAT CAGTCCCCAG
AATGTGGATG TTAATGTGCA CCCCACAAAG CATGAAGTTC ACTTCCTGCA CGAGGAGAGC
ATCCTGGAGC GGGTGCAGCA GCACATCGAG AGCAAGCTCC TGGGCTCCAA TTCCTCCAGG
ATGTACTTCA CCCAGACTTT GCTACCAGGA CTTGCTGGCC CCTCTGGGGA GATGGTTAAA
TCCACAACAA GTCTGACCTC GTCTTCTACT TCTGGAAGTA GTGATAAGGT CTATGCCCAC
CAGATGGTTC GTACAGATTC CCGGGAACAG AAGCTTGATG CATTTCTGCA GCCTCTGAGC
AAACCCCTGT CCAGTCAGCC CCAGGCCATT GTCACAGAGG ATAAGACAGA TATTTCTAGT
GGCAGGGCTA GGCAGCAAGA TGAGGAGATG CTTGAACTCC CAGCCCCTGC TGAAGTGGCT
GCCAAAAATC AGAGCTTGGA GGGGGATACA ACAAAGGGGA CTTCAGAAAT GTCAGAGAAG
AGAGGACCTA CTTCCAGCAA CCCCAGAAAG AGACATCGGG AAGATTCTGA TGTGGAAATG
GTGGAAGATG ATTCCCGAAA GGAAATGACT GCAGCTTGTA CCCCCCGGAG AAGGATCATT
AACCTCACTA GTGTTTTGAG TCTCCAGGAA GAAATTAATG AGCAGGGACA TGAGGTTCTC
CGGGAGATGT TGCATAACCA CTCCTTCGTG GGCTGTGTGA ATCCTCAGTG GGCCTTGGCA
CAGCATCAAA CCAAGTTATA CCTTCTCAAC ACCACCAAGC TTAGTGAAGA ACTGTTCTAC
CAGATACTCA TTTATGATTT TGCCAATTTT GGTGTTCTCA GGTTATCGGA GCCAGCACCG
CTCTTTGACC TTGCCATGCT TGCCTTAGAT AGTCCAGAGA GTGGCTGGAC AGAGGAAGAT
GGTCCCAAAG AAGGACTTGC TGAATACATT GTTGAGTTTC TGAAGAAGAA GGCTGAGATG
CTTGCAGACT ATTTCTCTTT GGAAATTGAT GAGGAAGGGA ACCTGATTGG ATTACCCCTT
CTGATTGACA ACTATGTGCC CCCTTTGGAG GGACTGCCTA TCTTCATTCT TCGACTAGCC
ACTGAGGTGA ATTGGGACGA AGAAAAGGAA TGTTTTGAAA GCCTCAGTAA AGAATGCGCT
ATGTTCTATT CCATCCGGAA GCAGTACATA TCTGAGGAGT CGACCCTCTC AGGCCAGCAG
AGTGAAGTGC CTGGCTCCAT TCCAAACTCC TGGAAGTGGA CTGTGGAACA CATTGTCTAT
AAAGCCTTGC GCTCACACAT TCTGCCTCCT AAACATTTCA CAGAAGATGG AAATATCCTG
CAGCTTGCTA ACCTGCCTGA TCTATACAAA GTCTTTGAGA GGTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20930
Clone ID Related Accession (Same CDS sequence) NM_001167618 , NM_001167619 , NM_001258273 , NM_001258274 , XM_005265163 , XM_005265164
Accession Version XM_005265164.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1548bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA mismatch repair protein Mlh1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATGGTT ACATATCCAA TGCAAACTAC TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA ATCAACTTCC TTGAGAAAAG CCATAGAAAC AGTGTATGCA
GCCTATTTGC CCAAAAACAC ACACCCATTC CTGTACCTCA GTTTAGAAAT CAGTCCCCAG
AATGTGGATG TTAATGTGCA CCCCACAAAG CATGAAGTTC ACTTCCTGCA CGAGGAGAGC
ATCCTGGAGC GGGTGCAGCA GCACATCGAG AGCAAGCTCC TGGGCTCCAA TTCCTCCAGG
ATGTACTTCA CCCAGACTTT GCTACCAGGA CTTGCTGGCC CCTCTGGGGA GATGGTTAAA
TCCACAACAA GTCTGACCTC GTCTTCTACT TCTGGAAGTA GTGATAAGGT CTATGCCCAC
CAGATGGTTC GTACAGATTC CCGGGAACAG AAGCTTGATG CATTTCTGCA GCCTCTGAGC
AAACCCCTGT CCAGTCAGCC CCAGGCCATT GTCACAGAGG ATAAGACAGA TATTTCTAGT
GGCAGGGCTA GGCAGCAAGA TGAGGAGATG CTTGAACTCC CAGCCCCTGC TGAAGTGGCT
GCCAAAAATC AGAGCTTGGA GGGGGATACA ACAAAGGGGA CTTCAGAAAT GTCAGAGAAG
AGAGGACCTA CTTCCAGCAA CCCCAGAAAG AGACATCGGG AAGATTCTGA TGTGGAAATG
GTGGAAGATG ATTCCCGAAA GGAAATGACT GCAGCTTGTA CCCCCCGGAG AAGGATCATT
AACCTCACTA GTGTTTTGAG TCTCCAGGAA GAAATTAATG AGCAGGGACA TGAGGTTCTC
CGGGAGATGT TGCATAACCA CTCCTTCGTG GGCTGTGTGA ATCCTCAGTG GGCCTTGGCA
CAGCATCAAA CCAAGTTATA CCTTCTCAAC ACCACCAAGC TTAGTGAAGA ACTGTTCTAC
CAGATACTCA TTTATGATTT TGCCAATTTT GGTGTTCTCA GGTTATCGGA GCCAGCACCG
CTCTTTGACC TTGCCATGCT TGCCTTAGAT AGTCCAGAGA GTGGCTGGAC AGAGGAAGAT
GGTCCCAAAG AAGGACTTGC TGAATACATT GTTGAGTTTC TGAAGAAGAA GGCTGAGATG
CTTGCAGACT ATTTCTCTTT GGAAATTGAT GAGGAAGGGA ACCTGATTGG ATTACCCCTT
CTGATTGACA ACTATGTGCC CCCTTTGGAG GGACTGCCTA TCTTCATTCT TCGACTAGCC
ACTGAGGTGA ATTGGGACGA AGAAAAGGAA TGTTTTGAAA GCCTCAGTAA AGAATGCGCT
ATGTTCTATT CCATCCGGAA GCAGTACATA TCTGAGGAGT CGACCCTCTC AGGCCAGCAG
AGTGAAGTGC CTGGCTCCAT TCCAAACTCC TGGAAGTGGA CTGTGGAACA CATTGTCTAT
AAAGCCTTGC GCTCACACAT TCTGCCTCCT AAACATTTCA CAGAAGATGG AAATATCCTG
CAGCTTGCTA ACCTGCCTGA TCTATACAAA GTCTTTGAGA GGTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265221.1
CDS775..2322
Misc Feature(1)775..1056(+)
Misc Feature(2)778..2112(+)
Misc Feature(3)982..984(+)
Translation

Target ORF information:

RefSeq Version XM_005265164
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mutL homolog 1 (MLH1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005265164

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATGGTT ACATATCCAA TGCAAACTAC TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA ATCAACTTCC TTGAGAAAAG CCATAGAAAC AGTGTATGCA
GCCTATTTGC CCAAAAACAC ACACCCATTC CTGTACCTCA GTTTAGAAAT CAGTCCCCAG
AATGTGGATG TTAATGTGCA CCCCACAAAG CATGAAGTTC ACTTCCTGCA CGAGGAGAGC
ATCCTGGAGC GGGTGCAGCA GCACATCGAG AGCAAGCTCC TGGGCTCCAA TTCCTCCAGG
ATGTACTTCA CCCAGACTTT GCTACCAGGA CTTGCTGGCC CCTCTGGGGA GATGGTTAAA
TCCACAACAA GTCTGACCTC GTCTTCTACT TCTGGAAGTA GTGATAAGGT CTATGCCCAC
CAGATGGTTC GTACAGATTC CCGGGAACAG AAGCTTGATG CATTTCTGCA GCCTCTGAGC
AAACCCCTGT CCAGTCAGCC CCAGGCCATT GTCACAGAGG ATAAGACAGA TATTTCTAGT
GGCAGGGCTA GGCAGCAAGA TGAGGAGATG CTTGAACTCC CAGCCCCTGC TGAAGTGGCT
GCCAAAAATC AGAGCTTGGA GGGGGATACA ACAAAGGGGA CTTCAGAAAT GTCAGAGAAG
AGAGGACCTA CTTCCAGCAA CCCCAGAAAG AGACATCGGG AAGATTCTGA TGTGGAAATG
GTGGAAGATG ATTCCCGAAA GGAAATGACT GCAGCTTGTA CCCCCCGGAG AAGGATCATT
AACCTCACTA GTGTTTTGAG TCTCCAGGAA GAAATTAATG AGCAGGGACA TGAGGTTCTC
CGGGAGATGT TGCATAACCA CTCCTTCGTG GGCTGTGTGA ATCCTCAGTG GGCCTTGGCA
CAGCATCAAA CCAAGTTATA CCTTCTCAAC ACCACCAAGC TTAGTGAAGA ACTGTTCTAC
CAGATACTCA TTTATGATTT TGCCAATTTT GGTGTTCTCA GGTTATCGGA GCCAGCACCG
CTCTTTGACC TTGCCATGCT TGCCTTAGAT AGTCCAGAGA GTGGCTGGAC AGAGGAAGAT
GGTCCCAAAG AAGGACTTGC TGAATACATT GTTGAGTTTC TGAAGAAGAA GGCTGAGATG
CTTGCAGACT ATTTCTCTTT GGAAATTGAT GAGGAAGGGA ACCTGATTGG ATTACCCCTT
CTGATTGACA ACTATGTGCC CCCTTTGGAG GGACTGCCTA TCTTCATTCT TCGACTAGCC
ACTGAGGTGA ATTGGGACGA AGAAAAGGAA TGTTTTGAAA GCCTCAGTAA AGAATGCGCT
ATGTTCTATT CCATCCGGAA GCAGTACATA TCTGAGGAGT CGACCCTCTC AGGCCAGCAG
AGTGAAGTGC CTGGCTCCAT TCCAAACTCC TGGAAGTGGA CTGTGGAACA CATTGTCTAT
AAAGCCTTGC GCTCACACAT TCTGCCTCCT AAACATTTCA CAGAAGATGG AAATATCCTG
CAGCTTGCTA ACCTGCCTGA TCTATACAAA GTCTTTGAGA GGTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37256
Clone ID Related Accession (Same CDS sequence) XM_005265166
Accession Version XM_005265166.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1248bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA mismatch repair protein Mlh1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTACTTCA CCCAGACTTT GCTACCAGGA CTTGCTGGCC CCTCTGGGGA GATGGTTAAA 
TCCACAACAA GTCTGACCTC GTCTTCTACT TCTGGAAGTA GTGATAAGGT CTATGCCCAC
CAGATGGTTC GTACAGATTC CCGGGAACAG AAGCTTGATG CATTTCTGCA GCCTCTGAGC
AAACCCCTGT CCAGTCAGCC CCAGGCCATT GTCACAGAGG ATAAGACAGA TATTTCTAGT
GGCAGGGCTA GGCAGCAAGA TGAGGAGATG CTTGAACTCC CAGCCCCTGC TGAAGTGGCT
GCCAAAAATC AGAGCTTGGA GGGGGATACA ACAAAGGGGA CTTCAGAAAT GTCAGAGAAG
AGAGGACCTA CTTCCAGCAA CCCCAGAAAG AGACATCGGG AAGATTCTGA TGTGGAAATG
GTGGAAGATG ATTCCCGAAA GGAAATGACT GCAGCTTGTA CCCCCCGGAG AAGGATCATT
AACCTCACTA GTGTTTTGAG TCTCCAGGAA GAAATTAATG AGCAGGGACA TGAGGTTCTC
CGGGAGATGT TGCATAACCA CTCCTTCGTG GGCTGTGTGA ATCCTCAGTG GGCCTTGGCA
CAGCATCAAA CCAAGTTATA CCTTCTCAAC ACCACCAAGC TTAGTGAAGA ACTGTTCTAC
CAGATACTCA TTTATGATTT TGCCAATTTT GGTGTTCTCA GGTTATCGGA GCCAGCACCG
CTCTTTGACC TTGCCATGCT TGCCTTAGAT AGTCCAGAGA GTGGCTGGAC AGAGGAAGAT
GGTCCCAAAG AAGGACTTGC TGAATACATT GTTGAGTTTC TGAAGAAGAA GGCTGAGATG
CTTGCAGACT ATTTCTCTTT GGAAATTGAT GAGGAAGGGA ACCTGATTGG ATTACCCCTT
CTGATTGACA ACTATGTGCC CCCTTTGGAG GGACTGCCTA TCTTCATTCT TCGACTAGCC
ACTGAGGTGA ATTGGGACGA AGAAAAGGAA TGTTTTGAAA GCCTCAGTAA AGAATGCGCT
ATGTTCTATT CCATCCGGAA GCAGTACATA TCTGAGGAGT CGACCCTCTC AGGCCAGCAG
AGTGAAGTGC CTGGCTCCAT TCCAAACTCC TGGAAGTGGA CTGTGGAACA CATTGTCTAT
AAAGCCTTGC GCTCACACAT TCTGCCTCCT AAACATTTCA CAGAAGATGG AAATATCCTG
CAGCTTGCTA ACCTGCCTGA TCTATACAAA GTCTTTGAGA GGTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265223.1
CDS936..2183
Translation

Target ORF information:

RefSeq Version XM_005265166
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mutL homolog 1 (MLH1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005265166

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTACTTCA CCCAGACTTT GCTACCAGGA CTTGCTGGCC CCTCTGGGGA GATGGTTAAA 
TCCACAACAA GTCTGACCTC GTCTTCTACT TCTGGAAGTA GTGATAAGGT CTATGCCCAC
CAGATGGTTC GTACAGATTC CCGGGAACAG AAGCTTGATG CATTTCTGCA GCCTCTGAGC
AAACCCCTGT CCAGTCAGCC CCAGGCCATT GTCACAGAGG ATAAGACAGA TATTTCTAGT
GGCAGGGCTA GGCAGCAAGA TGAGGAGATG CTTGAACTCC CAGCCCCTGC TGAAGTGGCT
GCCAAAAATC AGAGCTTGGA GGGGGATACA ACAAAGGGGA CTTCAGAAAT GTCAGAGAAG
AGAGGACCTA CTTCCAGCAA CCCCAGAAAG AGACATCGGG AAGATTCTGA TGTGGAAATG
GTGGAAGATG ATTCCCGAAA GGAAATGACT GCAGCTTGTA CCCCCCGGAG AAGGATCATT
AACCTCACTA GTGTTTTGAG TCTCCAGGAA GAAATTAATG AGCAGGGACA TGAGGTTCTC
CGGGAGATGT TGCATAACCA CTCCTTCGTG GGCTGTGTGA ATCCTCAGTG GGCCTTGGCA
CAGCATCAAA CCAAGTTATA CCTTCTCAAC ACCACCAAGC TTAGTGAAGA ACTGTTCTAC
CAGATACTCA TTTATGATTT TGCCAATTTT GGTGTTCTCA GGTTATCGGA GCCAGCACCG
CTCTTTGACC TTGCCATGCT TGCCTTAGAT AGTCCAGAGA GTGGCTGGAC AGAGGAAGAT
GGTCCCAAAG AAGGACTTGC TGAATACATT GTTGAGTTTC TGAAGAAGAA GGCTGAGATG
CTTGCAGACT ATTTCTCTTT GGAAATTGAT GAGGAAGGGA ACCTGATTGG ATTACCCCTT
CTGATTGACA ACTATGTGCC CCCTTTGGAG GGACTGCCTA TCTTCATTCT TCGACTAGCC
ACTGAGGTGA ATTGGGACGA AGAAAAGGAA TGTTTTGAAA GCCTCAGTAA AGAATGCGCT
ATGTTCTATT CCATCCGGAA GCAGTACATA TCTGAGGAGT CGACCCTCTC AGGCCAGCAG
AGTGAAGTGC CTGGCTCCAT TCCAAACTCC TGGAAGTGGA CTGTGGAACA CATTGTCTAT
AAAGCCTTGC GCTCACACAT TCTGCCTCCT AAACATTTCA CAGAAGATGG AAATATCCTG
CAGCTTGCTA ACCTGCCTGA TCTATACAAA GTCTTTGAGA GGTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58984
Clone ID Related Accession (Same CDS sequence) XM_011533727
Accession Version XM_011533727.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1197bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA mismatch repair protein Mlh1 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGTTAAAT CCACAACAAG TCTGACCTCG TCTTCTACTT CTGGAAGTAG TGATAAGGTC 
TATGCCCACC AGATGGTTCG TACAGATTCC CGGGAACAGA AGCTTGATGC ATTTCTGCAG
CCTCTGAGCA AACCCCTGTC CAGTCAGCCC CAGGCCATTG TCACAGAGGA TAAGACAGAT
ATTTCTAGTG GCAGGGCTAG GCAGCAAGAT GAGGAGATGC TTGAACTCCC AGCCCCTGCT
GAAGTGGCTG CCAAAAATCA GAGCTTGGAG GGGGATACAA CAAAGGGGAC TTCAGAAATG
TCAGAGAAGA GAGGACCTAC TTCCAGCAAC CCCAGAAAGA GACATCGGGA AGATTCTGAT
GTGGAAATGG TGGAAGATGA TTCCCGAAAG GAAATGACTG CAGCTTGTAC CCCCCGGAGA
AGGATCATTA ACCTCACTAG TGTTTTGAGT CTCCAGGAAG AAATTAATGA GCAGGGACAT
GAGGTTCTCC GGGAGATGTT GCATAACCAC TCCTTCGTGG GCTGTGTGAA TCCTCAGTGG
GCCTTGGCAC AGCATCAAAC CAAGTTATAC CTTCTCAACA CCACCAAGCT TAGTGAAGAA
CTGTTCTACC AGATACTCAT TTATGATTTT GCCAATTTTG GTGTTCTCAG GTTATCGGAG
CCAGCACCGC TCTTTGACCT TGCCATGCTT GCCTTAGATA GTCCAGAGAG TGGCTGGACA
GAGGAAGATG GTCCCAAAGA AGGACTTGCT GAATACATTG TTGAGTTTCT GAAGAAGAAG
GCTGAGATGC TTGCAGACTA TTTCTCTTTG GAAATTGATG AGGAAGGGAA CCTGATTGGA
TTACCCCTTC TGATTGACAA CTATGTGCCC CCTTTGGAGG GACTGCCTAT CTTCATTCTT
CGACTAGCCA CTGAGGTGAA TTGGGACGAA GAAAAGGAAT GTTTTGAAAG CCTCAGTAAA
GAATGCGCTA TGTTCTATTC CATCCGGAAG CAGTACATAT CTGAGGAGTC GACCCTCTCA
GGCCAGCAGA GTGAAGTGCC TGGCTCCATT CCAAACTCCT GGAAGTGGAC TGTGGAACAC
ATTGTCTATA AAGCCTTGCG CTCACACATT CTGCCTCCTA AACATTTCAC AGAAGATGGA
AATATCCTGC AGCTTGCTAA CCTGCCTGAT CTATACAAAG TCTTTGAGAG GTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011532029.1
CDS833..2029
Translation

Target ORF information:

RefSeq Version XM_011533727
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mutL homolog 1 (MLH1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011533727

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGTTAAAT CCACAACAAG TCTGACCTCG TCTTCTACTT CTGGAAGTAG TGATAAGGTC 
TATGCCCACC AGATGGTTCG TACAGATTCC CGGGAACAGA AGCTTGATGC ATTTCTGCAG
CCTCTGAGCA AACCCCTGTC CAGTCAGCCC CAGGCCATTG TCACAGAGGA TAAGACAGAT
ATTTCTAGTG GCAGGGCTAG GCAGCAAGAT GAGGAGATGC TTGAACTCCC AGCCCCTGCT
GAAGTGGCTG CCAAAAATCA GAGCTTGGAG GGGGATACAA CAAAGGGGAC TTCAGAAATG
TCAGAGAAGA GAGGACCTAC TTCCAGCAAC CCCAGAAAGA GACATCGGGA AGATTCTGAT
GTGGAAATGG TGGAAGATGA TTCCCGAAAG GAAATGACTG CAGCTTGTAC CCCCCGGAGA
AGGATCATTA ACCTCACTAG TGTTTTGAGT CTCCAGGAAG AAATTAATGA GCAGGGACAT
GAGGTTCTCC GGGAGATGTT GCATAACCAC TCCTTCGTGG GCTGTGTGAA TCCTCAGTGG
GCCTTGGCAC AGCATCAAAC CAAGTTATAC CTTCTCAACA CCACCAAGCT TAGTGAAGAA
CTGTTCTACC AGATACTCAT TTATGATTTT GCCAATTTTG GTGTTCTCAG GTTATCGGAG
CCAGCACCGC TCTTTGACCT TGCCATGCTT GCCTTAGATA GTCCAGAGAG TGGCTGGACA
GAGGAAGATG GTCCCAAAGA AGGACTTGCT GAATACATTG TTGAGTTTCT GAAGAAGAAG
GCTGAGATGC TTGCAGACTA TTTCTCTTTG GAAATTGATG AGGAAGGGAA CCTGATTGGA
TTACCCCTTC TGATTGACAA CTATGTGCCC CCTTTGGAGG GACTGCCTAT CTTCATTCTT
CGACTAGCCA CTGAGGTGAA TTGGGACGAA GAAAAGGAAT GTTTTGAAAG CCTCAGTAAA
GAATGCGCTA TGTTCTATTC CATCCGGAAG CAGTACATAT CTGAGGAGTC GACCCTCTCA
GGCCAGCAGA GTGAAGTGCC TGGCTCCATT CCAAACTCCT GGAAGTGGAC TGTGGAACAC
ATTGTCTATA AAGCCTTGCG CTCACACATT CTGCCTCCTA AACATTTCAC AGAAGATGGA
AATATCCTGC AGCTTGCTAA CCTGCCTGAT CTATACAAAG TCTTTGAGAG GTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20930
Clone ID Related Accession (Same CDS sequence) NM_001167618 , NM_001167619 , NM_001258273 , NM_001258274 , XM_005265163 , XM_005265164
Accession Version NM_001258274.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1548bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product DNA mismatch repair protein Mlh1 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX648844.1, AC011816.17 and BC006850.1. Transcript Variant: This variant (7) differs in the 5' UTR and contains an alternate exon compared to variant 1, that causes a frameshift. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Variants 3, 4, 6, and 7 all encode the same isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX648844.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188, SAMEA962333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATGGTT ACATATCCAA TGCAAACTAC TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA ATCAACTTCC TTGAGAAAAG CCATAGAAAC AGTGTATGCA
GCCTATTTGC CCAAAAACAC ACACCCATTC CTGTACCTCA GTTTAGAAAT CAGTCCCCAG
AATGTGGATG TTAATGTGCA CCCCACAAAG CATGAAGTTC ACTTCCTGCA CGAGGAGAGC
ATCCTGGAGC GGGTGCAGCA GCACATCGAG AGCAAGCTCC TGGGCTCCAA TTCCTCCAGG
ATGTACTTCA CCCAGACTTT GCTACCAGGA CTTGCTGGCC CCTCTGGGGA GATGGTTAAA
TCCACAACAA GTCTGACCTC GTCTTCTACT TCTGGAAGTA GTGATAAGGT CTATGCCCAC
CAGATGGTTC GTACAGATTC CCGGGAACAG AAGCTTGATG CATTTCTGCA GCCTCTGAGC
AAACCCCTGT CCAGTCAGCC CCAGGCCATT GTCACAGAGG ATAAGACAGA TATTTCTAGT
GGCAGGGCTA GGCAGCAAGA TGAGGAGATG CTTGAACTCC CAGCCCCTGC TGAAGTGGCT
GCCAAAAATC AGAGCTTGGA GGGGGATACA ACAAAGGGGA CTTCAGAAAT GTCAGAGAAG
AGAGGACCTA CTTCCAGCAA CCCCAGAAAG AGACATCGGG AAGATTCTGA TGTGGAAATG
GTGGAAGATG ATTCCCGAAA GGAAATGACT GCAGCTTGTA CCCCCCGGAG AAGGATCATT
AACCTCACTA GTGTTTTGAG TCTCCAGGAA GAAATTAATG AGCAGGGACA TGAGGTTCTC
CGGGAGATGT TGCATAACCA CTCCTTCGTG GGCTGTGTGA ATCCTCAGTG GGCCTTGGCA
CAGCATCAAA CCAAGTTATA CCTTCTCAAC ACCACCAAGC TTAGTGAAGA ACTGTTCTAC
CAGATACTCA TTTATGATTT TGCCAATTTT GGTGTTCTCA GGTTATCGGA GCCAGCACCG
CTCTTTGACC TTGCCATGCT TGCCTTAGAT AGTCCAGAGA GTGGCTGGAC AGAGGAAGAT
GGTCCCAAAG AAGGACTTGC TGAATACATT GTTGAGTTTC TGAAGAAGAA GGCTGAGATG
CTTGCAGACT ATTTCTCTTT GGAAATTGAT GAGGAAGGGA ACCTGATTGG ATTACCCCTT
CTGATTGACA ACTATGTGCC CCCTTTGGAG GGACTGCCTA TCTTCATTCT TCGACTAGCC
ACTGAGGTGA ATTGGGACGA AGAAAAGGAA TGTTTTGAAA GCCTCAGTAA AGAATGCGCT
ATGTTCTATT CCATCCGGAA GCAGTACATA TCTGAGGAGT CGACCCTCTC AGGCCAGCAG
AGTGAAGTGC CTGGCTCCAT TCCAAACTCC TGGAAGTGGA CTGTGGAACA CATTGTCTAT
AAAGCCTTGC GCTCACACAT TCTGCCTCCT AAACATTTCA CAGAAGATGG AAATATCCTG
CAGCTTGCTA ACCTGCCTGA TCTATACAAA GTCTTTGAGA GGTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001245203.1
CDS867..2414
Misc Feature(1)318..320(+)
Misc Feature(2)867..1148(+)
Misc Feature(3)870..2204(+)
Misc Feature(4)1074..1076(+)
Exon (1)1..114
Gene:MLH1
Gene Synonym:
Exon (2)115..205
Gene:MLH1
Gene Synonym:
Exon (3)206..350
Gene:MLH1
Gene Synonym:
Exon (4)351..449
Gene:MLH1
Gene Synonym:
Exon (5)450..523
Gene:MLH1
Gene Synonym:
Exon (6)524..596
Gene:MLH1
Gene Synonym:
Exon (7)597..688
Gene:MLH1
Gene Synonym:
Exon (8)689..731
Gene:MLH1
Gene Synonym:
Exon (9)732..820
Gene:MLH1
Gene Synonym:
Exon (10)821..933
Gene:MLH1
Gene Synonym:
Exon (11)934..1027
Gene:MLH1
Gene Synonym:
Exon (12)1028..1181
Gene:MLH1
Gene Synonym:
Exon (13)1182..1552
Gene:MLH1
Gene Synonym:
Exon (14)1553..1701
Gene:MLH1
Gene Synonym:
Exon (15)1702..1810
Gene:MLH1
Gene Synonym:
Exon (16)1811..1874
Gene:MLH1
Gene Synonym:
Exon (17)1875..2039
Gene:MLH1
Gene Synonym:
Exon (18)2040..2132
Gene:MLH1
Gene Synonym:
Exon (19)2133..2246
Gene:MLH1
Gene Synonym:
Exon (20)2247..2607
Gene:MLH1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001258274
Organism Homo sapiens (human)
Definition Homo sapiens mutL homolog 1 (MLH1), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001258274

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATGGTT ACATATCCAA TGCAAACTAC TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA ATCAACTTCC TTGAGAAAAG CCATAGAAAC AGTGTATGCA
GCCTATTTGC CCAAAAACAC ACACCCATTC CTGTACCTCA GTTTAGAAAT CAGTCCCCAG
AATGTGGATG TTAATGTGCA CCCCACAAAG CATGAAGTTC ACTTCCTGCA CGAGGAGAGC
ATCCTGGAGC GGGTGCAGCA GCACATCGAG AGCAAGCTCC TGGGCTCCAA TTCCTCCAGG
ATGTACTTCA CCCAGACTTT GCTACCAGGA CTTGCTGGCC CCTCTGGGGA GATGGTTAAA
TCCACAACAA GTCTGACCTC GTCTTCTACT TCTGGAAGTA GTGATAAGGT CTATGCCCAC
CAGATGGTTC GTACAGATTC CCGGGAACAG AAGCTTGATG CATTTCTGCA GCCTCTGAGC
AAACCCCTGT CCAGTCAGCC CCAGGCCATT GTCACAGAGG ATAAGACAGA TATTTCTAGT
GGCAGGGCTA GGCAGCAAGA TGAGGAGATG CTTGAACTCC CAGCCCCTGC TGAAGTGGCT
GCCAAAAATC AGAGCTTGGA GGGGGATACA ACAAAGGGGA CTTCAGAAAT GTCAGAGAAG
AGAGGACCTA CTTCCAGCAA CCCCAGAAAG AGACATCGGG AAGATTCTGA TGTGGAAATG
GTGGAAGATG ATTCCCGAAA GGAAATGACT GCAGCTTGTA CCCCCCGGAG AAGGATCATT
AACCTCACTA GTGTTTTGAG TCTCCAGGAA GAAATTAATG AGCAGGGACA TGAGGTTCTC
CGGGAGATGT TGCATAACCA CTCCTTCGTG GGCTGTGTGA ATCCTCAGTG GGCCTTGGCA
CAGCATCAAA CCAAGTTATA CCTTCTCAAC ACCACCAAGC TTAGTGAAGA ACTGTTCTAC
CAGATACTCA TTTATGATTT TGCCAATTTT GGTGTTCTCA GGTTATCGGA GCCAGCACCG
CTCTTTGACC TTGCCATGCT TGCCTTAGAT AGTCCAGAGA GTGGCTGGAC AGAGGAAGAT
GGTCCCAAAG AAGGACTTGC TGAATACATT GTTGAGTTTC TGAAGAAGAA GGCTGAGATG
CTTGCAGACT ATTTCTCTTT GGAAATTGAT GAGGAAGGGA ACCTGATTGG ATTACCCCTT
CTGATTGACA ACTATGTGCC CCCTTTGGAG GGACTGCCTA TCTTCATTCT TCGACTAGCC
ACTGAGGTGA ATTGGGACGA AGAAAAGGAA TGTTTTGAAA GCCTCAGTAA AGAATGCGCT
ATGTTCTATT CCATCCGGAA GCAGTACATA TCTGAGGAGT CGACCCTCTC AGGCCAGCAG
AGTGAAGTGC CTGGCTCCAT TCCAAACTCC TGGAAGTGGA CTGTGGAACA CATTGTCTAT
AAAGCCTTGC GCTCACACAT TCTGCCTCCT AAACATTTCA CAGAAGATGG AAATATCCTG
CAGCTTGCTA ACCTGCCTGA TCTATACAAA GTCTTTGAGA GGTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27476
Clone ID Related Accession (Same CDS sequence) NM_001258271
Accession Version NM_001258271.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2064bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product DNA mismatch repair protein Mlh1 isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN414955.1, AB209848.1 and BC006850.1. Transcript Variant: This variant (5) lacks two alternate in-frame exons compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209848.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142670, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGTCGTTCG TGGCAGGGGT TATTCGGCGG CTGGACGAGA CAGTGGTGAA CCGCATCGCG 
GCGGGGGAAG TTATCCAGCG GCCAGCTAAT GCTATCAAAG AGATGATTGA GAACTGTTTA
GATGCAAAAT CCACAAGTAT TCAAGTGATT GTTAAAGAGG GAGGCCTGAA GTTGATTCAG
ATCCAAGACA ATGGCACCGG GATCAGGAAA GAAGATCTGG ATATTGTATG TGAAAGGTTC
ACTACTAGTA AACTGCAGTC CTTTGAGGAT TTAGCCAGTA TTTCTACCTA TGGCTTTCGA
GGTGAGGCTT TGGCCAGCAT AAGCCATGTG GCTCATGTTA CTATTACAAC GAAAACAGCT
GATGGAAAGT GTGCATACAG AGCAAGTTAC TCAGATGGAA AACTGAAAGC CCCTCCTAAA
CCATGTGCTG GCAATCAAGG GACCCAGATC ACGGTGGAGG ACCTTTTTTA CAACATAGCC
ACGAGGAGAA AAGCTTTAAA AAATCCAAGT GAAGAATATG GGAAAATTTT GGAAGTTGTT
GGCAGGTATT CAGTACACAA TGCAGGCATT AGTTTCTCAG TTAAAAAACA AGGAGAGACA
GTAGCTGATG TTAGGACACT ACCCAATGCC TCAACCGTGG ACAATATTCG CTCCATCTTT
GGAAATGCTG TTAGTCGAGA ACTGATAGAA ATTGGATGTG AGGATAAAAC CCTAGCCTTC
AAAATGAATG GTTACATATC CAATGCAAAC TACTCAGTGA AGAAGTGCAT CTTCTTACTC
TTCATCAACC ATCGTCTGGT AGAATCAACT TCCTTGAGAA AAGCCATAGA AACAGTGTAT
GCAGCCTATT TGCCCAAAAA CACACACCCA TTCCTGTACC TCAGTTTAGA AATCAGTCCC
CAGAATGTGG ATGTTAATGT GCACCCCACA AAGCATGAAG TTCACTTCCT GCACGAGGAG
AGCATCCTGG AGCGGGTGCA GCAGCACATC GAGAGCAAGC TCCTGGGCTC CAATTCCTCC
AGGATGTACT TCACCCAGAC TTTGCTACCA GGACTTGCTG GCCCCTCTGG GGAGATGGTT
AAATCCACAA CAAGTCTGAC CTCGTCTTCT ACTTCTGGAA GTAGTGATAA GGTCTATGCC
CACCAGATGG TTCGTACAGA TTCCCGGGAA CAGAAGCTTG ATGCATTTCT GCAGCCTCTG
AGCAAACCCC TGTCCAGTCA GCCCCAGGCC ATTGTCACAG AGGATAAGAC AGATATTTCT
AGTGGCAGGG CTAGGCAGCA AGATGAGGAG ATGCTTGAAC TCCCAGCCCC TGCTGAAGTG
GCTGCCAAAA ATCAGAGCTT GGAGGGGGAT ACAACAAAGG GGACTTCAGA AATGTCAGAG
AAGAGAGGAC CTACTTCCAG CAACCCCAGA AAGAGACATC GGGAAGATTC TGATGTGGAA
ATGGTGGAAG ATGATTCCCG AAAGGAAATG ACTGCAGCTT GTACCCCCCG GAGAAGGATC
ATTAACCTCA CTAGTGTTTT GAGTCTCCAG GAAGAAATTA ATGAGCAGGG ACATGAGGTT
CTCCGGGAGA TGTTGCATAA CCACTCCTTC GTGGGCTGTG TGAATCCTCA GTGGGCCTTG
GCACAGCATC AAACCAAGTT ATACCTTCTC AACACCACCA AGCTTAGTGA AGAACTGTTC
TACCAGATAC TCATTTATGA TTTTGCCAAT TTTGGTGTTC TCAGGTTATC GGAGCCAGCA
CCGCTCTTTG ACCTTGCCAT GCTTGCCTTA GATAGTCCAG AGAGTGGCTG GACAGAGGAA
GATGGTCCCA AAGAAGGACT TGCTGAATAC ATTGTTGAGT TTCTGAAGAA GAAGGCTGAG
ATGCTTGCAG ACTATTTCTC TTTGGAAATT GATGAGAGTG AAGTGCCTGG CTCCATTCCA
AACTCCTGGA AGTGGACTGT GGAACACATT GTCTATAAAG CCTTGCGCTC ACACATTCTG
CCTCCTAAAC ATTTCACAGA AGATGGAAAT ATCCTGCAGC TTGCTAACCT GCCTGATCTA
TACAAAGTCT TTGAGAGGTG TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001245200.1
CDS199..2262
Misc Feature(1)160..162(+)
Misc Feature(2)202..204(+)
Misc Feature(3)214..1143(+)
Misc Feature(4)289..564(+)
Misc Feature(5)298..564(+)
Misc Feature(6)310..312(+)
Misc Feature(7)391..507(+)
Misc Feature(8)829..1203(+)
Misc Feature(9)1129..1131(+)
Misc Feature(10)1627..1629(+)
Exon (1)1..314
Gene:MLH1
Gene Synonym:
Exon (2)315..405
Gene:MLH1
Gene Synonym:
Exon (3)406..504
Gene:MLH1
Gene Synonym:
Exon (4)505..578
Gene:MLH1
Gene Synonym:
Exon (5)579..651
Gene:MLH1
Gene Synonym:
Exon (6)652..743
Gene:MLH1
Gene Synonym:
Exon (7)744..786
Gene:MLH1
Gene Synonym:
Exon (8)787..875
Gene:MLH1
Gene Synonym:
Exon (9)876..988
Gene:MLH1
Gene Synonym:
Exon (10)989..1082
Gene:MLH1
Gene Synonym:
Exon (11)1083..1236
Gene:MLH1
Gene Synonym:
Exon (12)1237..1607
Gene:MLH1
Gene Synonym:
Exon (13)1608..1756
Gene:MLH1
Gene Synonym:
Exon (14)1757..1865
Gene:MLH1
Gene Synonym:
Exon (15)1866..1929
Gene:MLH1
Gene Synonym:
Exon (16)1930..2094
Gene:MLH1
Gene Synonym:
Exon (17)2095..2455
Gene:MLH1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001258271
Organism Homo sapiens (human)
Definition Homo sapiens mutL homolog 1 (MLH1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001258271

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGTCGTTCG TGGCAGGGGT TATTCGGCGG CTGGACGAGA CAGTGGTGAA CCGCATCGCG 
GCGGGGGAAG TTATCCAGCG GCCAGCTAAT GCTATCAAAG AGATGATTGA GAACTGTTTA
GATGCAAAAT CCACAAGTAT TCAAGTGATT GTTAAAGAGG GAGGCCTGAA GTTGATTCAG
ATCCAAGACA ATGGCACCGG GATCAGGAAA GAAGATCTGG ATATTGTATG TGAAAGGTTC
ACTACTAGTA AACTGCAGTC CTTTGAGGAT TTAGCCAGTA TTTCTACCTA TGGCTTTCGA
GGTGAGGCTT TGGCCAGCAT AAGCCATGTG GCTCATGTTA CTATTACAAC GAAAACAGCT
GATGGAAAGT GTGCATACAG AGCAAGTTAC TCAGATGGAA AACTGAAAGC CCCTCCTAAA
CCATGTGCTG GCAATCAAGG GACCCAGATC ACGGTGGAGG ACCTTTTTTA CAACATAGCC
ACGAGGAGAA AAGCTTTAAA AAATCCAAGT GAAGAATATG GGAAAATTTT GGAAGTTGTT
GGCAGGTATT CAGTACACAA TGCAGGCATT AGTTTCTCAG TTAAAAAACA AGGAGAGACA
GTAGCTGATG TTAGGACACT ACCCAATGCC TCAACCGTGG ACAATATTCG CTCCATCTTT
GGAAATGCTG TTAGTCGAGA ACTGATAGAA ATTGGATGTG AGGATAAAAC CCTAGCCTTC
AAAATGAATG GTTACATATC CAATGCAAAC TACTCAGTGA AGAAGTGCAT CTTCTTACTC
TTCATCAACC ATCGTCTGGT AGAATCAACT TCCTTGAGAA AAGCCATAGA AACAGTGTAT
GCAGCCTATT TGCCCAAAAA CACACACCCA TTCCTGTACC TCAGTTTAGA AATCAGTCCC
CAGAATGTGG ATGTTAATGT GCACCCCACA AAGCATGAAG TTCACTTCCT GCACGAGGAG
AGCATCCTGG AGCGGGTGCA GCAGCACATC GAGAGCAAGC TCCTGGGCTC CAATTCCTCC
AGGATGTACT TCACCCAGAC TTTGCTACCA GGACTTGCTG GCCCCTCTGG GGAGATGGTT
AAATCCACAA CAAGTCTGAC CTCGTCTTCT ACTTCTGGAA GTAGTGATAA GGTCTATGCC
CACCAGATGG TTCGTACAGA TTCCCGGGAA CAGAAGCTTG ATGCATTTCT GCAGCCTCTG
AGCAAACCCC TGTCCAGTCA GCCCCAGGCC ATTGTCACAG AGGATAAGAC AGATATTTCT
AGTGGCAGGG CTAGGCAGCA AGATGAGGAG ATGCTTGAAC TCCCAGCCCC TGCTGAAGTG
GCTGCCAAAA ATCAGAGCTT GGAGGGGGAT ACAACAAAGG GGACTTCAGA AATGTCAGAG
AAGAGAGGAC CTACTTCCAG CAACCCCAGA AAGAGACATC GGGAAGATTC TGATGTGGAA
ATGGTGGAAG ATGATTCCCG AAAGGAAATG ACTGCAGCTT GTACCCCCCG GAGAAGGATC
ATTAACCTCA CTAGTGTTTT GAGTCTCCAG GAAGAAATTA ATGAGCAGGG ACATGAGGTT
CTCCGGGAGA TGTTGCATAA CCACTCCTTC GTGGGCTGTG TGAATCCTCA GTGGGCCTTG
GCACAGCATC AAACCAAGTT ATACCTTCTC AACACCACCA AGCTTAGTGA AGAACTGTTC
TACCAGATAC TCATTTATGA TTTTGCCAAT TTTGGTGTTC TCAGGTTATC GGAGCCAGCA
CCGCTCTTTG ACCTTGCCAT GCTTGCCTTA GATAGTCCAG AGAGTGGCTG GACAGAGGAA
GATGGTCCCA AAGAAGGACT TGCTGAATAC ATTGTTGAGT TTCTGAAGAA GAAGGCTGAG
ATGCTTGCAG ACTATTTCTC TTTGGAAATT GATGAGAGTG AAGTGCCTGG CTCCATTCCA
AACTCCTGGA AGTGGACTGT GGAACACATT GTCTATAAAG CCTTGCGCTC ACACATTCTG
CCTCCTAAAC ATTTCACAGA AGATGGAAAT ATCCTGCAGC TTGCTAACCT GCCTGATCTA
TACAAAGTCT TTGAGAGGTG TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20930
Clone ID Related Accession (Same CDS sequence) NM_001167618 , NM_001167619 , NM_001258273 , NM_001258274 , XM_005265163 , XM_005265164
Accession Version NM_001258273.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1548bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product DNA mismatch repair protein Mlh1 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN414955.1, AK316264.1 and BC006850.1. Transcript Variant: This variant (6) lacks an alternate exon compared to variant 1, that causes a frameshift. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Variants 3, 4, 6, and 7 all encode the same isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK316264.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153946, SAMEA2154529 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATGGTT ACATATCCAA TGCAAACTAC TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA ATCAACTTCC TTGAGAAAAG CCATAGAAAC AGTGTATGCA
GCCTATTTGC CCAAAAACAC ACACCCATTC CTGTACCTCA GTTTAGAAAT CAGTCCCCAG
AATGTGGATG TTAATGTGCA CCCCACAAAG CATGAAGTTC ACTTCCTGCA CGAGGAGAGC
ATCCTGGAGC GGGTGCAGCA GCACATCGAG AGCAAGCTCC TGGGCTCCAA TTCCTCCAGG
ATGTACTTCA CCCAGACTTT GCTACCAGGA CTTGCTGGCC CCTCTGGGGA GATGGTTAAA
TCCACAACAA GTCTGACCTC GTCTTCTACT TCTGGAAGTA GTGATAAGGT CTATGCCCAC
CAGATGGTTC GTACAGATTC CCGGGAACAG AAGCTTGATG CATTTCTGCA GCCTCTGAGC
AAACCCCTGT CCAGTCAGCC CCAGGCCATT GTCACAGAGG ATAAGACAGA TATTTCTAGT
GGCAGGGCTA GGCAGCAAGA TGAGGAGATG CTTGAACTCC CAGCCCCTGC TGAAGTGGCT
GCCAAAAATC AGAGCTTGGA GGGGGATACA ACAAAGGGGA CTTCAGAAAT GTCAGAGAAG
AGAGGACCTA CTTCCAGCAA CCCCAGAAAG AGACATCGGG AAGATTCTGA TGTGGAAATG
GTGGAAGATG ATTCCCGAAA GGAAATGACT GCAGCTTGTA CCCCCCGGAG AAGGATCATT
AACCTCACTA GTGTTTTGAG TCTCCAGGAA GAAATTAATG AGCAGGGACA TGAGGTTCTC
CGGGAGATGT TGCATAACCA CTCCTTCGTG GGCTGTGTGA ATCCTCAGTG GGCCTTGGCA
CAGCATCAAA CCAAGTTATA CCTTCTCAAC ACCACCAAGC TTAGTGAAGA ACTGTTCTAC
CAGATACTCA TTTATGATTT TGCCAATTTT GGTGTTCTCA GGTTATCGGA GCCAGCACCG
CTCTTTGACC TTGCCATGCT TGCCTTAGAT AGTCCAGAGA GTGGCTGGAC AGAGGAAGAT
GGTCCCAAAG AAGGACTTGC TGAATACATT GTTGAGTTTC TGAAGAAGAA GGCTGAGATG
CTTGCAGACT ATTTCTCTTT GGAAATTGAT GAGGAAGGGA ACCTGATTGG ATTACCCCTT
CTGATTGACA ACTATGTGCC CCCTTTGGAG GGACTGCCTA TCTTCATTCT TCGACTAGCC
ACTGAGGTGA ATTGGGACGA AGAAAAGGAA TGTTTTGAAA GCCTCAGTAA AGAATGCGCT
ATGTTCTATT CCATCCGGAA GCAGTACATA TCTGAGGAGT CGACCCTCTC AGGCCAGCAG
AGTGAAGTGC CTGGCTCCAT TCCAAACTCC TGGAAGTGGA CTGTGGAACA CATTGTCTAT
AAAGCCTTGC GCTCACACAT TCTGCCTCCT AAACATTTCA CAGAAGATGG AAATATCCTG
CAGCTTGCTA ACCTGCCTGA TCTATACAAA GTCTTTGAGA GGTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001245202.1
CDS831..2378
Misc Feature(1)306..308(+)
Misc Feature(2)831..1112(+)
Misc Feature(3)834..2168(+)
Misc Feature(4)1038..1040(+)
Exon (1)1..314
Gene:MLH1
Gene Synonym:
Exon (2)315..413
Gene:MLH1
Gene Synonym:
Exon (3)414..487
Gene:MLH1
Gene Synonym:
Exon (4)488..560
Gene:MLH1
Gene Synonym:
Exon (5)561..652
Gene:MLH1
Gene Synonym:
Exon (6)653..695
Gene:MLH1
Gene Synonym:
Exon (7)696..784
Gene:MLH1
Gene Synonym:
Exon (8)785..897
Gene:MLH1
Gene Synonym:
Exon (9)898..991
Gene:MLH1
Gene Synonym:
Exon (10)992..1145
Gene:MLH1
Gene Synonym:
Exon (11)1146..1516
Gene:MLH1
Gene Synonym:
Exon (12)1517..1665
Gene:MLH1
Gene Synonym:
Exon (13)1666..1774
Gene:MLH1
Gene Synonym:
Exon (14)1775..1838
Gene:MLH1
Gene Synonym:
Exon (15)1839..2003
Gene:MLH1
Gene Synonym:
Exon (16)2004..2096
Gene:MLH1
Gene Synonym:
Exon (17)2097..2210
Gene:MLH1
Gene Synonym:
Exon (18)2211..2571
Gene:MLH1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001258273
Organism Homo sapiens (human)
Definition Homo sapiens mutL homolog 1 (MLH1), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001258273

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATGGTT ACATATCCAA TGCAAACTAC TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA ATCAACTTCC TTGAGAAAAG CCATAGAAAC AGTGTATGCA
GCCTATTTGC CCAAAAACAC ACACCCATTC CTGTACCTCA GTTTAGAAAT CAGTCCCCAG
AATGTGGATG TTAATGTGCA CCCCACAAAG CATGAAGTTC ACTTCCTGCA CGAGGAGAGC
ATCCTGGAGC GGGTGCAGCA GCACATCGAG AGCAAGCTCC TGGGCTCCAA TTCCTCCAGG
ATGTACTTCA CCCAGACTTT GCTACCAGGA CTTGCTGGCC CCTCTGGGGA GATGGTTAAA
TCCACAACAA GTCTGACCTC GTCTTCTACT TCTGGAAGTA GTGATAAGGT CTATGCCCAC
CAGATGGTTC GTACAGATTC CCGGGAACAG AAGCTTGATG CATTTCTGCA GCCTCTGAGC
AAACCCCTGT CCAGTCAGCC CCAGGCCATT GTCACAGAGG ATAAGACAGA TATTTCTAGT
GGCAGGGCTA GGCAGCAAGA TGAGGAGATG CTTGAACTCC CAGCCCCTGC TGAAGTGGCT
GCCAAAAATC AGAGCTTGGA GGGGGATACA ACAAAGGGGA CTTCAGAAAT GTCAGAGAAG
AGAGGACCTA CTTCCAGCAA CCCCAGAAAG AGACATCGGG AAGATTCTGA TGTGGAAATG
GTGGAAGATG ATTCCCGAAA GGAAATGACT GCAGCTTGTA CCCCCCGGAG AAGGATCATT
AACCTCACTA GTGTTTTGAG TCTCCAGGAA GAAATTAATG AGCAGGGACA TGAGGTTCTC
CGGGAGATGT TGCATAACCA CTCCTTCGTG GGCTGTGTGA ATCCTCAGTG GGCCTTGGCA
CAGCATCAAA CCAAGTTATA CCTTCTCAAC ACCACCAAGC TTAGTGAAGA ACTGTTCTAC
CAGATACTCA TTTATGATTT TGCCAATTTT GGTGTTCTCA GGTTATCGGA GCCAGCACCG
CTCTTTGACC TTGCCATGCT TGCCTTAGAT AGTCCAGAGA GTGGCTGGAC AGAGGAAGAT
GGTCCCAAAG AAGGACTTGC TGAATACATT GTTGAGTTTC TGAAGAAGAA GGCTGAGATG
CTTGCAGACT ATTTCTCTTT GGAAATTGAT GAGGAAGGGA ACCTGATTGG ATTACCCCTT
CTGATTGACA ACTATGTGCC CCCTTTGGAG GGACTGCCTA TCTTCATTCT TCGACTAGCC
ACTGAGGTGA ATTGGGACGA AGAAAAGGAA TGTTTTGAAA GCCTCAGTAA AGAATGCGCT
ATGTTCTATT CCATCCGGAA GCAGTACATA TCTGAGGAGT CGACCCTCTC AGGCCAGCAG
AGTGAAGTGC CTGGCTCCAT TCCAAACTCC TGGAAGTGGA CTGTGGAACA CATTGTCTAT
AAAGCCTTGC GCTCACACAT TCTGCCTCCT AAACATTTCA CAGAAGATGG AAATATCCTG
CAGCTTGCTA ACCTGCCTGA TCTATACAAA GTCTTTGAGA GGTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26603
Clone ID Related Accession (Same CDS sequence) NM_000249
Accession Version NM_000249.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2271bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product DNA mismatch repair protein Mlh1 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN414955.1 and BC006850.1. This sequence is a reference standard in the RefSeqGene project. On Nov 6, 2009 this sequence version replaced gi:28559089. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006850.1, U07418.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTCGTTCG TGGCAGGGGT TATTCGGCGG CTGGACGAGA CAGTGGTGAA CCGCATCGCG 
GCGGGGGAAG TTATCCAGCG GCCAGCTAAT GCTATCAAAG AGATGATTGA GAACTGTTTA
GATGCAAAAT CCACAAGTAT TCAAGTGATT GTTAAAGAGG GAGGCCTGAA GTTGATTCAG
ATCCAAGACA ATGGCACCGG GATCAGGAAA GAAGATCTGG ATATTGTATG TGAAAGGTTC
ACTACTAGTA AACTGCAGTC CTTTGAGGAT TTAGCCAGTA TTTCTACCTA TGGCTTTCGA
GGTGAGGCTT TGGCCAGCAT AAGCCATGTG GCTCATGTTA CTATTACAAC GAAAACAGCT
GATGGAAAGT GTGCATACAG AGCAAGTTAC TCAGATGGAA AACTGAAAGC CCCTCCTAAA
CCATGTGCTG GCAATCAAGG GACCCAGATC ACGGTGGAGG ACCTTTTTTA CAACATAGCC
ACGAGGAGAA AAGCTTTAAA AAATCCAAGT GAAGAATATG GGAAAATTTT GGAAGTTGTT
GGCAGGTATT CAGTACACAA TGCAGGCATT AGTTTCTCAG TTAAAAAACA AGGAGAGACA
GTAGCTGATG TTAGGACACT ACCCAATGCC TCAACCGTGG ACAATATTCG CTCCATCTTT
GGAAATGCTG TTAGTCGAGA ACTGATAGAA ATTGGATGTG AGGATAAAAC CCTAGCCTTC
AAAATGAATG GTTACATATC CAATGCAAAC TACTCAGTGA AGAAGTGCAT CTTCTTACTC
TTCATCAACC ATCGTCTGGT AGAATCAACT TCCTTGAGAA AAGCCATAGA AACAGTGTAT
GCAGCCTATT TGCCCAAAAA CACACACCCA TTCCTGTACC TCAGTTTAGA AATCAGTCCC
CAGAATGTGG ATGTTAATGT GCACCCCACA AAGCATGAAG TTCACTTCCT GCACGAGGAG
AGCATCCTGG AGCGGGTGCA GCAGCACATC GAGAGCAAGC TCCTGGGCTC CAATTCCTCC
AGGATGTACT TCACCCAGAC TTTGCTACCA GGACTTGCTG GCCCCTCTGG GGAGATGGTT
AAATCCACAA CAAGTCTGAC CTCGTCTTCT ACTTCTGGAA GTAGTGATAA GGTCTATGCC
CACCAGATGG TTCGTACAGA TTCCCGGGAA CAGAAGCTTG ATGCATTTCT GCAGCCTCTG
AGCAAACCCC TGTCCAGTCA GCCCCAGGCC ATTGTCACAG AGGATAAGAC AGATATTTCT
AGTGGCAGGG CTAGGCAGCA AGATGAGGAG ATGCTTGAAC TCCCAGCCCC TGCTGAAGTG
GCTGCCAAAA ATCAGAGCTT GGAGGGGGAT ACAACAAAGG GGACTTCAGA AATGTCAGAG
AAGAGAGGAC CTACTTCCAG CAACCCCAGA AAGAGACATC GGGAAGATTC TGATGTGGAA
ATGGTGGAAG ATGATTCCCG AAAGGAAATG ACTGCAGCTT GTACCCCCCG GAGAAGGATC
ATTAACCTCA CTAGTGTTTT GAGTCTCCAG GAAGAAATTA ATGAGCAGGG ACATGAGGTT
CTCCGGGAGA TGTTGCATAA CCACTCCTTC GTGGGCTGTG TGAATCCTCA GTGGGCCTTG
GCACAGCATC AAACCAAGTT ATACCTTCTC AACACCACCA AGCTTAGTGA AGAACTGTTC
TACCAGATAC TCATTTATGA TTTTGCCAAT TTTGGTGTTC TCAGGTTATC GGAGCCAGCA
CCGCTCTTTG ACCTTGCCAT GCTTGCCTTA GATAGTCCAG AGAGTGGCTG GACAGAGGAA
GATGGTCCCA AAGAAGGACT TGCTGAATAC ATTGTTGAGT TTCTGAAGAA GAAGGCTGAG
ATGCTTGCAG ACTATTTCTC TTTGGAAATT GATGAGGAAG GGAACCTGAT TGGATTACCC
CTTCTGATTG ACAACTATGT GCCCCCTTTG GAGGGACTGC CTATCTTCAT TCTTCGACTA
GCCACTGAGG TGAATTGGGA CGAAGAAAAG GAATGTTTTG AAAGCCTCAG TAAAGAATGC
GCTATGTTCT ATTCCATCCG GAAGCAGTAC ATATCTGAGG AGTCGACCCT CTCAGGCCAG
CAGAGTGAAG TGCCTGGCTC CATTCCAAAC TCCTGGAAGT GGACTGTGGA ACACATTGTC
TATAAAGCCT TGCGCTCACA CATTCTGCCT CCTAAACATT TCACAGAAGA TGGAAATATC
CTGCAGCTTG CTAACCTGCC TGATCTATAC AAAGTCTTTG AGAGGTGTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000240.1
CDS199..2469
Misc Feature(1)160..162(+)
Misc Feature(2)202..204(+)
Misc Feature(3)214..1143(+)
Misc Feature(4)289..564(+)
Misc Feature(5)298..564(+)
Misc Feature(6)310..312(+)
Misc Feature(7)391..507(+)
Misc Feature(8)829..1203(+)
Misc Feature(9)1129..1131(+)
Misc Feature(10)1426..2148(+)
Misc Feature(11)1450..1452(+)
Misc Feature(12)1627..1629(+)
Exon (1)1..314
Gene:MLH1
Gene Synonym:
Exon (2)315..405
Gene:MLH1
Gene Synonym:
Exon (3)406..504
Gene:MLH1
Gene Synonym:
Exon (4)505..578
Gene:MLH1
Gene Synonym:
Exon (5)579..651
Gene:MLH1
Gene Synonym:
Exon (6)652..743
Gene:MLH1
Gene Synonym:
Exon (7)744..786
Gene:MLH1
Gene Synonym:
Exon (8)787..875
Gene:MLH1
Gene Synonym:
Exon (9)876..988
Gene:MLH1
Gene Synonym:
Exon (10)989..1082
Gene:MLH1
Gene Synonym:
Exon (11)1083..1236
Gene:MLH1
Gene Synonym:
Exon (12)1237..1607
Gene:MLH1
Gene Synonym:
Exon (13)1608..1756
Gene:MLH1
Gene Synonym:
Exon (14)1757..1865
Gene:MLH1
Gene Synonym:
Exon (15)1866..1929
Gene:MLH1
Gene Synonym:
Exon (16)1930..2094
Gene:MLH1
Gene Synonym:
Exon (17)2095..2187
Gene:MLH1
Gene Synonym:
Exon (18)2188..2301
Gene:MLH1
Gene Synonym:
Exon (19)2302..2662
Gene:MLH1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000249
Organism Homo sapiens (human)
Definition Homo sapiens mutL homolog 1 (MLH1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000249

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTCGTTCG TGGCAGGGGT TATTCGGCGG CTGGACGAGA CAGTGGTGAA CCGCATCGCG 
GCGGGGGAAG TTATCCAGCG GCCAGCTAAT GCTATCAAAG AGATGATTGA GAACTGTTTA
GATGCAAAAT CCACAAGTAT TCAAGTGATT GTTAAAGAGG GAGGCCTGAA GTTGATTCAG
ATCCAAGACA ATGGCACCGG GATCAGGAAA GAAGATCTGG ATATTGTATG TGAAAGGTTC
ACTACTAGTA AACTGCAGTC CTTTGAGGAT TTAGCCAGTA TTTCTACCTA TGGCTTTCGA
GGTGAGGCTT TGGCCAGCAT AAGCCATGTG GCTCATGTTA CTATTACAAC GAAAACAGCT
GATGGAAAGT GTGCATACAG AGCAAGTTAC TCAGATGGAA AACTGAAAGC CCCTCCTAAA
CCATGTGCTG GCAATCAAGG GACCCAGATC ACGGTGGAGG ACCTTTTTTA CAACATAGCC
ACGAGGAGAA AAGCTTTAAA AAATCCAAGT GAAGAATATG GGAAAATTTT GGAAGTTGTT
GGCAGGTATT CAGTACACAA TGCAGGCATT AGTTTCTCAG TTAAAAAACA AGGAGAGACA
GTAGCTGATG TTAGGACACT ACCCAATGCC TCAACCGTGG ACAATATTCG CTCCATCTTT
GGAAATGCTG TTAGTCGAGA ACTGATAGAA ATTGGATGTG AGGATAAAAC CCTAGCCTTC
AAAATGAATG GTTACATATC CAATGCAAAC TACTCAGTGA AGAAGTGCAT CTTCTTACTC
TTCATCAACC ATCGTCTGGT AGAATCAACT TCCTTGAGAA AAGCCATAGA AACAGTGTAT
GCAGCCTATT TGCCCAAAAA CACACACCCA TTCCTGTACC TCAGTTTAGA AATCAGTCCC
CAGAATGTGG ATGTTAATGT GCACCCCACA AAGCATGAAG TTCACTTCCT GCACGAGGAG
AGCATCCTGG AGCGGGTGCA GCAGCACATC GAGAGCAAGC TCCTGGGCTC CAATTCCTCC
AGGATGTACT TCACCCAGAC TTTGCTACCA GGACTTGCTG GCCCCTCTGG GGAGATGGTT
AAATCCACAA CAAGTCTGAC CTCGTCTTCT ACTTCTGGAA GTAGTGATAA GGTCTATGCC
CACCAGATGG TTCGTACAGA TTCCCGGGAA CAGAAGCTTG ATGCATTTCT GCAGCCTCTG
AGCAAACCCC TGTCCAGTCA GCCCCAGGCC ATTGTCACAG AGGATAAGAC AGATATTTCT
AGTGGCAGGG CTAGGCAGCA AGATGAGGAG ATGCTTGAAC TCCCAGCCCC TGCTGAAGTG
GCTGCCAAAA ATCAGAGCTT GGAGGGGGAT ACAACAAAGG GGACTTCAGA AATGTCAGAG
AAGAGAGGAC CTACTTCCAG CAACCCCAGA AAGAGACATC GGGAAGATTC TGATGTGGAA
ATGGTGGAAG ATGATTCCCG AAAGGAAATG ACTGCAGCTT GTACCCCCCG GAGAAGGATC
ATTAACCTCA CTAGTGTTTT GAGTCTCCAG GAAGAAATTA ATGAGCAGGG ACATGAGGTT
CTCCGGGAGA TGTTGCATAA CCACTCCTTC GTGGGCTGTG TGAATCCTCA GTGGGCCTTG
GCACAGCATC AAACCAAGTT ATACCTTCTC AACACCACCA AGCTTAGTGA AGAACTGTTC
TACCAGATAC TCATTTATGA TTTTGCCAAT TTTGGTGTTC TCAGGTTATC GGAGCCAGCA
CCGCTCTTTG ACCTTGCCAT GCTTGCCTTA GATAGTCCAG AGAGTGGCTG GACAGAGGAA
GATGGTCCCA AAGAAGGACT TGCTGAATAC ATTGTTGAGT TTCTGAAGAA GAAGGCTGAG
ATGCTTGCAG ACTATTTCTC TTTGGAAATT GATGAGGAAG GGAACCTGAT TGGATTACCC
CTTCTGATTG ACAACTATGT GCCCCCTTTG GAGGGACTGC CTATCTTCAT TCTTCGACTA
GCCACTGAGG TGAATTGGGA CGAAGAAAAG GAATGTTTTG AAAGCCTCAG TAAAGAATGC
GCTATGTTCT ATTCCATCCG GAAGCAGTAC ATATCTGAGG AGTCGACCCT CTCAGGCCAG
CAGAGTGAAG TGCCTGGCTC CATTCCAAAC TCCTGGAAGT GGACTGTGGA ACACATTGTC
TATAAAGCCT TGCGCTCACA CATTCTGCCT CCTAAACATT TCACAGAAGA TGGAAATATC
CTGCAGCTTG CTAACCTGCC TGATCTATAC AAAGTCTTTG AGAGGTGTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27066
Clone ID Related Accession (Same CDS sequence) NM_001167617
Accession Version NM_001167617.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1977bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product DNA mismatch repair protein Mlh1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA517219.1, AK298583.1 and BC006850.1. Transcript Variant: This variant (2) contains a distinct 5'-terminal exon and therefore lacks an in-frame portion of the 5' coding region compared to variant 1. The resulting isoform (2) has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298583.1, AK298324.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCTTTCG AGGCTTTGGC CAGCATAAGC CATGTGGCTC ATGTTACTAT TACAACGAAA 
ACAGCTGATG GAAAGTGTGC ATACAGAGCA AGTTACTCAG ATGGAAAACT GAAAGCCCCT
CCTAAACCAT GTGCTGGCAA TCAAGGGACC CAGATCACGG TGGAGGACCT TTTTTACAAC
ATAGCCACGA GGAGAAAAGC TTTAAAAAAT CCAAGTGAAG AATATGGGAA AATTTTGGAA
GTTGTTGGCA GGTATTCAGT ACACAATGCA GGCATTAGTT TCTCAGTTAA AAAACAAGGA
GAGACAGTAG CTGATGTTAG GACACTACCC AATGCCTCAA CCGTGGACAA TATTCGCTCC
ATCTTTGGAA ATGCTGTTAG TCGAGAACTG ATAGAAATTG GATGTGAGGA TAAAACCCTA
GCCTTCAAAA TGAATGGTTA CATATCCAAT GCAAACTACT CAGTGAAGAA GTGCATCTTC
TTACTCTTCA TCAACCATCG TCTGGTAGAA TCAACTTCCT TGAGAAAAGC CATAGAAACA
GTGTATGCAG CCTATTTGCC CAAAAACACA CACCCATTCC TGTACCTCAG TTTAGAAATC
AGTCCCCAGA ATGTGGATGT TAATGTGCAC CCCACAAAGC ATGAAGTTCA CTTCCTGCAC
GAGGAGAGCA TCCTGGAGCG GGTGCAGCAG CACATCGAGA GCAAGCTCCT GGGCTCCAAT
TCCTCCAGGA TGTACTTCAC CCAGACTTTG CTACCAGGAC TTGCTGGCCC CTCTGGGGAG
ATGGTTAAAT CCACAACAAG TCTGACCTCG TCTTCTACTT CTGGAAGTAG TGATAAGGTC
TATGCCCACC AGATGGTTCG TACAGATTCC CGGGAACAGA AGCTTGATGC ATTTCTGCAG
CCTCTGAGCA AACCCCTGTC CAGTCAGCCC CAGGCCATTG TCACAGAGGA TAAGACAGAT
ATTTCTAGTG GCAGGGCTAG GCAGCAAGAT GAGGAGATGC TTGAACTCCC AGCCCCTGCT
GAAGTGGCTG CCAAAAATCA GAGCTTGGAG GGGGATACAA CAAAGGGGAC TTCAGAAATG
TCAGAGAAGA GAGGACCTAC TTCCAGCAAC CCCAGAAAGA GACATCGGGA AGATTCTGAT
GTGGAAATGG TGGAAGATGA TTCCCGAAAG GAAATGACTG CAGCTTGTAC CCCCCGGAGA
AGGATCATTA ACCTCACTAG TGTTTTGAGT CTCCAGGAAG AAATTAATGA GCAGGGACAT
GAGGTTCTCC GGGAGATGTT GCATAACCAC TCCTTCGTGG GCTGTGTGAA TCCTCAGTGG
GCCTTGGCAC AGCATCAAAC CAAGTTATAC CTTCTCAACA CCACCAAGCT TAGTGAAGAA
CTGTTCTACC AGATACTCAT TTATGATTTT GCCAATTTTG GTGTTCTCAG GTTATCGGAG
CCAGCACCGC TCTTTGACCT TGCCATGCTT GCCTTAGATA GTCCAGAGAG TGGCTGGACA
GAGGAAGATG GTCCCAAAGA AGGACTTGCT GAATACATTG TTGAGTTTCT GAAGAAGAAG
GCTGAGATGC TTGCAGACTA TTTCTCTTTG GAAATTGATG AGGAAGGGAA CCTGATTGGA
TTACCCCTTC TGATTGACAA CTATGTGCCC CCTTTGGAGG GACTGCCTAT CTTCATTCTT
CGACTAGCCA CTGAGGTGAA TTGGGACGAA GAAAAGGAAT GTTTTGAAAG CCTCAGTAAA
GAATGCGCTA TGTTCTATTC CATCCGGAAG CAGTACATAT CTGAGGAGTC GACCCTCTCA
GGCCAGCAGA GTGAAGTGCC TGGCTCCATT CCAAACTCCT GGAAGTGGAC TGTGGAACAC
ATTGTCTATA AAGCCTTGCG CTCACACATT CTGCCTCCTA AACATTTCAC AGAAGATGGA
AATATCCTGC AGCTTGCTAA CCTGCCTGAT CTATACAAAG TCTTTGAGAG GTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001161089.1
CDS288..2264
Misc Feature(1)270..272(+)
Misc Feature(2)297..938(+)
Misc Feature(3)624..998(+)
Misc Feature(4)924..926(+)
Exon (1)1..114
Gene:MLH1
Gene Synonym:
Exon (2)115..205
Gene:MLH1
Gene Synonym:
Exon (3)206..299
Gene:MLH1
Gene Synonym:
Exon (4)300..373
Gene:MLH1
Gene Synonym:
Exon (5)374..446
Gene:MLH1
Gene Synonym:
Exon (6)447..538
Gene:MLH1
Gene Synonym:
Exon (7)539..581
Gene:MLH1
Gene Synonym:
Exon (8)582..670
Gene:MLH1
Gene Synonym:
Exon (9)671..783
Gene:MLH1
Gene Synonym:
Exon (10)784..877
Gene:MLH1
Gene Synonym:
Exon (11)878..1031
Gene:MLH1
Gene Synonym:
Exon (12)1032..1402
Gene:MLH1
Gene Synonym:
Exon (13)1403..1551
Gene:MLH1
Gene Synonym:
Exon (14)1552..1660
Gene:MLH1
Gene Synonym:
Exon (15)1661..1724
Gene:MLH1
Gene Synonym:
Exon (16)1725..1889
Gene:MLH1
Gene Synonym:
Exon (17)1890..1982
Gene:MLH1
Gene Synonym:
Exon (18)1983..2096
Gene:MLH1
Gene Synonym:
Exon (19)2097..2457
Gene:MLH1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001167617
Organism Homo sapiens (human)
Definition Homo sapiens mutL homolog 1 (MLH1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001167617

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCTTTCG AGGCTTTGGC CAGCATAAGC CATGTGGCTC ATGTTACTAT TACAACGAAA 
ACAGCTGATG GAAAGTGTGC ATACAGAGCA AGTTACTCAG ATGGAAAACT GAAAGCCCCT
CCTAAACCAT GTGCTGGCAA TCAAGGGACC CAGATCACGG TGGAGGACCT TTTTTACAAC
ATAGCCACGA GGAGAAAAGC TTTAAAAAAT CCAAGTGAAG AATATGGGAA AATTTTGGAA
GTTGTTGGCA GGTATTCAGT ACACAATGCA GGCATTAGTT TCTCAGTTAA AAAACAAGGA
GAGACAGTAG CTGATGTTAG GACACTACCC AATGCCTCAA CCGTGGACAA TATTCGCTCC
ATCTTTGGAA ATGCTGTTAG TCGAGAACTG ATAGAAATTG GATGTGAGGA TAAAACCCTA
GCCTTCAAAA TGAATGGTTA CATATCCAAT GCAAACTACT CAGTGAAGAA GTGCATCTTC
TTACTCTTCA TCAACCATCG TCTGGTAGAA TCAACTTCCT TGAGAAAAGC CATAGAAACA
GTGTATGCAG CCTATTTGCC CAAAAACACA CACCCATTCC TGTACCTCAG TTTAGAAATC
AGTCCCCAGA ATGTGGATGT TAATGTGCAC CCCACAAAGC ATGAAGTTCA CTTCCTGCAC
GAGGAGAGCA TCCTGGAGCG GGTGCAGCAG CACATCGAGA GCAAGCTCCT GGGCTCCAAT
TCCTCCAGGA TGTACTTCAC CCAGACTTTG CTACCAGGAC TTGCTGGCCC CTCTGGGGAG
ATGGTTAAAT CCACAACAAG TCTGACCTCG TCTTCTACTT CTGGAAGTAG TGATAAGGTC
TATGCCCACC AGATGGTTCG TACAGATTCC CGGGAACAGA AGCTTGATGC ATTTCTGCAG
CCTCTGAGCA AACCCCTGTC CAGTCAGCCC CAGGCCATTG TCACAGAGGA TAAGACAGAT
ATTTCTAGTG GCAGGGCTAG GCAGCAAGAT GAGGAGATGC TTGAACTCCC AGCCCCTGCT
GAAGTGGCTG CCAAAAATCA GAGCTTGGAG GGGGATACAA CAAAGGGGAC TTCAGAAATG
TCAGAGAAGA GAGGACCTAC TTCCAGCAAC CCCAGAAAGA GACATCGGGA AGATTCTGAT
GTGGAAATGG TGGAAGATGA TTCCCGAAAG GAAATGACTG CAGCTTGTAC CCCCCGGAGA
AGGATCATTA ACCTCACTAG TGTTTTGAGT CTCCAGGAAG AAATTAATGA GCAGGGACAT
GAGGTTCTCC GGGAGATGTT GCATAACCAC TCCTTCGTGG GCTGTGTGAA TCCTCAGTGG
GCCTTGGCAC AGCATCAAAC CAAGTTATAC CTTCTCAACA CCACCAAGCT TAGTGAAGAA
CTGTTCTACC AGATACTCAT TTATGATTTT GCCAATTTTG GTGTTCTCAG GTTATCGGAG
CCAGCACCGC TCTTTGACCT TGCCATGCTT GCCTTAGATA GTCCAGAGAG TGGCTGGACA
GAGGAAGATG GTCCCAAAGA AGGACTTGCT GAATACATTG TTGAGTTTCT GAAGAAGAAG
GCTGAGATGC TTGCAGACTA TTTCTCTTTG GAAATTGATG AGGAAGGGAA CCTGATTGGA
TTACCCCTTC TGATTGACAA CTATGTGCCC CCTTTGGAGG GACTGCCTAT CTTCATTCTT
CGACTAGCCA CTGAGGTGAA TTGGGACGAA GAAAAGGAAT GTTTTGAAAG CCTCAGTAAA
GAATGCGCTA TGTTCTATTC CATCCGGAAG CAGTACATAT CTGAGGAGTC GACCCTCTCA
GGCCAGCAGA GTGAAGTGCC TGGCTCCATT CCAAACTCCT GGAAGTGGAC TGTGGAACAC
ATTGTCTATA AAGCCTTGCG CTCACACATT CTGCCTCCTA AACATTTCAC AGAAGATGGA
AATATCCTGC AGCTTGCTAA CCTGCCTGAT CTATACAAAG TCTTTGAGAG GTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20930
Clone ID Related Accession (Same CDS sequence) NM_001167618 , NM_001167619 , NM_001258273 , NM_001258274 , XM_005265163 , XM_005265164
Accession Version NM_001167618.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1548bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product DNA mismatch repair protein Mlh1 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA517219.1, AK295359.1 and BC006850.1. Transcript Variant: This variant (3) contains a distinct 5'-terminal exon and uses an alternate splice site in a 5' exon and therefore lacks an in-frame portion of the 5' coding region compared to variant 1. The resulting isoform (3) has a shorter N-terminus compared to isoform 1. Variants 3, 4, 6, and 7 all encode the same isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295359.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATGGTT ACATATCCAA TGCAAACTAC TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA ATCAACTTCC TTGAGAAAAG CCATAGAAAC AGTGTATGCA
GCCTATTTGC CCAAAAACAC ACACCCATTC CTGTACCTCA GTTTAGAAAT CAGTCCCCAG
AATGTGGATG TTAATGTGCA CCCCACAAAG CATGAAGTTC ACTTCCTGCA CGAGGAGAGC
ATCCTGGAGC GGGTGCAGCA GCACATCGAG AGCAAGCTCC TGGGCTCCAA TTCCTCCAGG
ATGTACTTCA CCCAGACTTT GCTACCAGGA CTTGCTGGCC CCTCTGGGGA GATGGTTAAA
TCCACAACAA GTCTGACCTC GTCTTCTACT TCTGGAAGTA GTGATAAGGT CTATGCCCAC
CAGATGGTTC GTACAGATTC CCGGGAACAG AAGCTTGATG CATTTCTGCA GCCTCTGAGC
AAACCCCTGT CCAGTCAGCC CCAGGCCATT GTCACAGAGG ATAAGACAGA TATTTCTAGT
GGCAGGGCTA GGCAGCAAGA TGAGGAGATG CTTGAACTCC CAGCCCCTGC TGAAGTGGCT
GCCAAAAATC AGAGCTTGGA GGGGGATACA ACAAAGGGGA CTTCAGAAAT GTCAGAGAAG
AGAGGACCTA CTTCCAGCAA CCCCAGAAAG AGACATCGGG AAGATTCTGA TGTGGAAATG
GTGGAAGATG ATTCCCGAAA GGAAATGACT GCAGCTTGTA CCCCCCGGAG AAGGATCATT
AACCTCACTA GTGTTTTGAG TCTCCAGGAA GAAATTAATG AGCAGGGACA TGAGGTTCTC
CGGGAGATGT TGCATAACCA CTCCTTCGTG GGCTGTGTGA ATCCTCAGTG GGCCTTGGCA
CAGCATCAAA CCAAGTTATA CCTTCTCAAC ACCACCAAGC TTAGTGAAGA ACTGTTCTAC
CAGATACTCA TTTATGATTT TGCCAATTTT GGTGTTCTCA GGTTATCGGA GCCAGCACCG
CTCTTTGACC TTGCCATGCT TGCCTTAGAT AGTCCAGAGA GTGGCTGGAC AGAGGAAGAT
GGTCCCAAAG AAGGACTTGC TGAATACATT GTTGAGTTTC TGAAGAAGAA GGCTGAGATG
CTTGCAGACT ATTTCTCTTT GGAAATTGAT GAGGAAGGGA ACCTGATTGG ATTACCCCTT
CTGATTGACA ACTATGTGCC CCCTTTGGAG GGACTGCCTA TCTTCATTCT TCGACTAGCC
ACTGAGGTGA ATTGGGACGA AGAAAAGGAA TGTTTTGAAA GCCTCAGTAA AGAATGCGCT
ATGTTCTATT CCATCCGGAA GCAGTACATA TCTGAGGAGT CGACCCTCTC AGGCCAGCAG
AGTGAAGTGC CTGGCTCCAT TCCAAACTCC TGGAAGTGGA CTGTGGAACA CATTGTCTAT
AAAGCCTTGC GCTCACACAT TCTGCCTCCT AAACATTTCA CAGAAGATGG AAATATCCTG
CAGCTTGCTA ACCTGCCTGA TCTATACAAA GTCTTTGAGA GGTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001161090.1
CDS717..2264
Misc Feature(1)27..29(+)
Misc Feature(2)717..998(+)
Misc Feature(3)720..2054(+)
Misc Feature(4)924..926(+)
Exon (1)1..114
Gene:MLH1
Gene Synonym:
Exon (2)115..200
Gene:MLH1
Gene Synonym:
Exon (3)201..299
Gene:MLH1
Gene Synonym:
Exon (4)300..373
Gene:MLH1
Gene Synonym:
Exon (5)374..446
Gene:MLH1
Gene Synonym:
Exon (6)447..538
Gene:MLH1
Gene Synonym:
Exon (7)539..581
Gene:MLH1
Gene Synonym:
Exon (8)582..670
Gene:MLH1
Gene Synonym:
Exon (9)671..783
Gene:MLH1
Gene Synonym:
Exon (10)784..877
Gene:MLH1
Gene Synonym:
Exon (11)878..1031
Gene:MLH1
Gene Synonym:
Exon (12)1032..1402
Gene:MLH1
Gene Synonym:
Exon (13)1403..1551
Gene:MLH1
Gene Synonym:
Exon (14)1552..1660
Gene:MLH1
Gene Synonym:
Exon (15)1661..1724
Gene:MLH1
Gene Synonym:
Exon (16)1725..1889
Gene:MLH1
Gene Synonym:
Exon (17)1890..1982
Gene:MLH1
Gene Synonym:
Exon (18)1983..2096
Gene:MLH1
Gene Synonym:
Exon (19)2097..2457
Gene:MLH1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001167618
Organism Homo sapiens (human)
Definition Homo sapiens mutL homolog 1 (MLH1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001167618

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATGGTT ACATATCCAA TGCAAACTAC TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA ATCAACTTCC TTGAGAAAAG CCATAGAAAC AGTGTATGCA
GCCTATTTGC CCAAAAACAC ACACCCATTC CTGTACCTCA GTTTAGAAAT CAGTCCCCAG
AATGTGGATG TTAATGTGCA CCCCACAAAG CATGAAGTTC ACTTCCTGCA CGAGGAGAGC
ATCCTGGAGC GGGTGCAGCA GCACATCGAG AGCAAGCTCC TGGGCTCCAA TTCCTCCAGG
ATGTACTTCA CCCAGACTTT GCTACCAGGA CTTGCTGGCC CCTCTGGGGA GATGGTTAAA
TCCACAACAA GTCTGACCTC GTCTTCTACT TCTGGAAGTA GTGATAAGGT CTATGCCCAC
CAGATGGTTC GTACAGATTC CCGGGAACAG AAGCTTGATG CATTTCTGCA GCCTCTGAGC
AAACCCCTGT CCAGTCAGCC CCAGGCCATT GTCACAGAGG ATAAGACAGA TATTTCTAGT
GGCAGGGCTA GGCAGCAAGA TGAGGAGATG CTTGAACTCC CAGCCCCTGC TGAAGTGGCT
GCCAAAAATC AGAGCTTGGA GGGGGATACA ACAAAGGGGA CTTCAGAAAT GTCAGAGAAG
AGAGGACCTA CTTCCAGCAA CCCCAGAAAG AGACATCGGG AAGATTCTGA TGTGGAAATG
GTGGAAGATG ATTCCCGAAA GGAAATGACT GCAGCTTGTA CCCCCCGGAG AAGGATCATT
AACCTCACTA GTGTTTTGAG TCTCCAGGAA GAAATTAATG AGCAGGGACA TGAGGTTCTC
CGGGAGATGT TGCATAACCA CTCCTTCGTG GGCTGTGTGA ATCCTCAGTG GGCCTTGGCA
CAGCATCAAA CCAAGTTATA CCTTCTCAAC ACCACCAAGC TTAGTGAAGA ACTGTTCTAC
CAGATACTCA TTTATGATTT TGCCAATTTT GGTGTTCTCA GGTTATCGGA GCCAGCACCG
CTCTTTGACC TTGCCATGCT TGCCTTAGAT AGTCCAGAGA GTGGCTGGAC AGAGGAAGAT
GGTCCCAAAG AAGGACTTGC TGAATACATT GTTGAGTTTC TGAAGAAGAA GGCTGAGATG
CTTGCAGACT ATTTCTCTTT GGAAATTGAT GAGGAAGGGA ACCTGATTGG ATTACCCCTT
CTGATTGACA ACTATGTGCC CCCTTTGGAG GGACTGCCTA TCTTCATTCT TCGACTAGCC
ACTGAGGTGA ATTGGGACGA AGAAAAGGAA TGTTTTGAAA GCCTCAGTAA AGAATGCGCT
ATGTTCTATT CCATCCGGAA GCAGTACATA TCTGAGGAGT CGACCCTCTC AGGCCAGCAG
AGTGAAGTGC CTGGCTCCAT TCCAAACTCC TGGAAGTGGA CTGTGGAACA CATTGTCTAT
AAAGCCTTGC GCTCACACAT TCTGCCTCCT AAACATTTCA CAGAAGATGG AAATATCCTG
CAGCTTGCTA ACCTGCCTGA TCTATACAAA GTCTTTGAGA GGTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20930
Clone ID Related Accession (Same CDS sequence) NM_001167618 , NM_001167619 , NM_001258273 , NM_001258274 , XM_005265163 , XM_005265164
Accession Version NM_001167619.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1548bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product DNA mismatch repair protein Mlh1 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA517219.1, AK316074.1 and BC006850.1. Transcript Variant: This variant (4) contains a distinct 5'-terminal exon and lacks a 5' exon and therefore lacks an in-frame portion of the 5' coding region compared to variant 1. The resulting isoform (3) has a shorter N-terminus compared to isoform 1. Variants 3, 4, 6, and 7 all encode the same isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK316074.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATGGTT ACATATCCAA TGCAAACTAC TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA ATCAACTTCC TTGAGAAAAG CCATAGAAAC AGTGTATGCA
GCCTATTTGC CCAAAAACAC ACACCCATTC CTGTACCTCA GTTTAGAAAT CAGTCCCCAG
AATGTGGATG TTAATGTGCA CCCCACAAAG CATGAAGTTC ACTTCCTGCA CGAGGAGAGC
ATCCTGGAGC GGGTGCAGCA GCACATCGAG AGCAAGCTCC TGGGCTCCAA TTCCTCCAGG
ATGTACTTCA CCCAGACTTT GCTACCAGGA CTTGCTGGCC CCTCTGGGGA GATGGTTAAA
TCCACAACAA GTCTGACCTC GTCTTCTACT TCTGGAAGTA GTGATAAGGT CTATGCCCAC
CAGATGGTTC GTACAGATTC CCGGGAACAG AAGCTTGATG CATTTCTGCA GCCTCTGAGC
AAACCCCTGT CCAGTCAGCC CCAGGCCATT GTCACAGAGG ATAAGACAGA TATTTCTAGT
GGCAGGGCTA GGCAGCAAGA TGAGGAGATG CTTGAACTCC CAGCCCCTGC TGAAGTGGCT
GCCAAAAATC AGAGCTTGGA GGGGGATACA ACAAAGGGGA CTTCAGAAAT GTCAGAGAAG
AGAGGACCTA CTTCCAGCAA CCCCAGAAAG AGACATCGGG AAGATTCTGA TGTGGAAATG
GTGGAAGATG ATTCCCGAAA GGAAATGACT GCAGCTTGTA CCCCCCGGAG AAGGATCATT
AACCTCACTA GTGTTTTGAG TCTCCAGGAA GAAATTAATG AGCAGGGACA TGAGGTTCTC
CGGGAGATGT TGCATAACCA CTCCTTCGTG GGCTGTGTGA ATCCTCAGTG GGCCTTGGCA
CAGCATCAAA CCAAGTTATA CCTTCTCAAC ACCACCAAGC TTAGTGAAGA ACTGTTCTAC
CAGATACTCA TTTATGATTT TGCCAATTTT GGTGTTCTCA GGTTATCGGA GCCAGCACCG
CTCTTTGACC TTGCCATGCT TGCCTTAGAT AGTCCAGAGA GTGGCTGGAC AGAGGAAGAT
GGTCCCAAAG AAGGACTTGC TGAATACATT GTTGAGTTTC TGAAGAAGAA GGCTGAGATG
CTTGCAGACT ATTTCTCTTT GGAAATTGAT GAGGAAGGGA ACCTGATTGG ATTACCCCTT
CTGATTGACA ACTATGTGCC CCCTTTGGAG GGACTGCCTA TCTTCATTCT TCGACTAGCC
ACTGAGGTGA ATTGGGACGA AGAAAAGGAA TGTTTTGAAA GCCTCAGTAA AGAATGCGCT
ATGTTCTATT CCATCCGGAA GCAGTACATA TCTGAGGAGT CGACCCTCTC AGGCCAGCAG
AGTGAAGTGC CTGGCTCCAT TCCAAACTCC TGGAAGTGGA CTGTGGAACA CATTGTCTAT
AAAGCCTTGC GCTCACACAT TCTGCCTCCT AAACATTTCA CAGAAGATGG AAATATCCTG
CAGCTTGCTA ACCTGCCTGA TCTATACAAA GTCTTTGAGA GGTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001161091.1
CDS630..2177
Misc Feature(1)402..404(+)
Misc Feature(2)630..911(+)
Misc Feature(3)633..1967(+)
Misc Feature(4)837..839(+)
Exon (1)1..114
Gene:MLH1
Gene Synonym:
Exon (2)115..205
Gene:MLH1
Gene Synonym:
Exon (3)206..304
Gene:MLH1
Gene Synonym:
Exon (4)305..378
Gene:MLH1
Gene Synonym:
Exon (5)379..451
Gene:MLH1
Gene Synonym:
Exon (6)452..494
Gene:MLH1
Gene Synonym:
Exon (7)495..583
Gene:MLH1
Gene Synonym:
Exon (8)584..696
Gene:MLH1
Gene Synonym:
Exon (9)697..790
Gene:MLH1
Gene Synonym:
Exon (10)791..944
Gene:MLH1
Gene Synonym:
Exon (11)945..1315
Gene:MLH1
Gene Synonym:
Exon (12)1316..1464
Gene:MLH1
Gene Synonym:
Exon (13)1465..1573
Gene:MLH1
Gene Synonym:
Exon (14)1574..1637
Gene:MLH1
Gene Synonym:
Exon (15)1638..1802
Gene:MLH1
Gene Synonym:
Exon (16)1803..1895
Gene:MLH1
Gene Synonym:
Exon (17)1896..2009
Gene:MLH1
Gene Synonym:
Exon (18)2010..2370
Gene:MLH1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001167619
Organism Homo sapiens (human)
Definition Homo sapiens mutL homolog 1 (MLH1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001167619

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATGGTT ACATATCCAA TGCAAACTAC TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA ATCAACTTCC TTGAGAAAAG CCATAGAAAC AGTGTATGCA
GCCTATTTGC CCAAAAACAC ACACCCATTC CTGTACCTCA GTTTAGAAAT CAGTCCCCAG
AATGTGGATG TTAATGTGCA CCCCACAAAG CATGAAGTTC ACTTCCTGCA CGAGGAGAGC
ATCCTGGAGC GGGTGCAGCA GCACATCGAG AGCAAGCTCC TGGGCTCCAA TTCCTCCAGG
ATGTACTTCA CCCAGACTTT GCTACCAGGA CTTGCTGGCC CCTCTGGGGA GATGGTTAAA
TCCACAACAA GTCTGACCTC GTCTTCTACT TCTGGAAGTA GTGATAAGGT CTATGCCCAC
CAGATGGTTC GTACAGATTC CCGGGAACAG AAGCTTGATG CATTTCTGCA GCCTCTGAGC
AAACCCCTGT CCAGTCAGCC CCAGGCCATT GTCACAGAGG ATAAGACAGA TATTTCTAGT
GGCAGGGCTA GGCAGCAAGA TGAGGAGATG CTTGAACTCC CAGCCCCTGC TGAAGTGGCT
GCCAAAAATC AGAGCTTGGA GGGGGATACA ACAAAGGGGA CTTCAGAAAT GTCAGAGAAG
AGAGGACCTA CTTCCAGCAA CCCCAGAAAG AGACATCGGG AAGATTCTGA TGTGGAAATG
GTGGAAGATG ATTCCCGAAA GGAAATGACT GCAGCTTGTA CCCCCCGGAG AAGGATCATT
AACCTCACTA GTGTTTTGAG TCTCCAGGAA GAAATTAATG AGCAGGGACA TGAGGTTCTC
CGGGAGATGT TGCATAACCA CTCCTTCGTG GGCTGTGTGA ATCCTCAGTG GGCCTTGGCA
CAGCATCAAA CCAAGTTATA CCTTCTCAAC ACCACCAAGC TTAGTGAAGA ACTGTTCTAC
CAGATACTCA TTTATGATTT TGCCAATTTT GGTGTTCTCA GGTTATCGGA GCCAGCACCG
CTCTTTGACC TTGCCATGCT TGCCTTAGAT AGTCCAGAGA GTGGCTGGAC AGAGGAAGAT
GGTCCCAAAG AAGGACTTGC TGAATACATT GTTGAGTTTC TGAAGAAGAA GGCTGAGATG
CTTGCAGACT ATTTCTCTTT GGAAATTGAT GAGGAAGGGA ACCTGATTGG ATTACCCCTT
CTGATTGACA ACTATGTGCC CCCTTTGGAG GGACTGCCTA TCTTCATTCT TCGACTAGCC
ACTGAGGTGA ATTGGGACGA AGAAAAGGAA TGTTTTGAAA GCCTCAGTAA AGAATGCGCT
ATGTTCTATT CCATCCGGAA GCAGTACATA TCTGAGGAGT CGACCCTCTC AGGCCAGCAG
AGTGAAGTGC CTGGCTCCAT TCCAAACTCC TGGAAGTGGA CTGTGGAACA CATTGTCTAT
AAAGCCTTGC GCTCACACAT TCTGCCTCCT AAACATTTCA CAGAAGATGG AAATATCCTG
CAGCTTGCTA ACCTGCCTGA TCTATACAAA GTCTTTGAGA GGTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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