×

MPL cDNA ORF clone, Homo sapiens (human)

Gene Symbol MPL
Entrez Gene ID 4352
Full Name MPL proto-oncogene, thrombopoietin receptor
Synonyms C-MPL, CD110, MPLV, THCYT2, TPOR
General protein information
Preferred Names
thrombopoietin receptor
Names
thrombopoietin receptor
TPO-R
proto-oncogene c-Mpl
myeloproliferative leukemia protein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p34

Summary In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

159530

Disorder Html: Thrombocytopenia, congenital amegakaryocytic, 604498 (3);





The following MPL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MPL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu59037 XM_011541478 PREDICTED: Homo sapiens MPL proto-oncogene, thrombopoietin receptor (MPL), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439.00
OHu28553 NM_005373 Homo sapiens MPL proto-oncogene, thrombopoietin receptor (MPL), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.30
$99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu59037
Accession Version XM_011541478.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1887bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product thrombopoietin receptor isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_032977.10) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCCCTCCT GGGCCCTCTT CATGGTCACC TCCTGCCTCC TCCTGGCCCC TCAAAACCTG 
GCCCAAGTCA GCAGCCAAGA CTCAGAGCCC CTGAAGTGTT TCTCCCGAAC ATTTGAGGAC
CTCACTTGCT TCTGGGATGA GGAAGAGGCA GCGCCCAGTG GGACATACCA GCTGCTGTAT
GCCTACCCGC GGGAGAAGCC CCGTGCTTGC CCCCTGAGTT CCCAGAGCAT GCCCCACTTT
GGAACCCGAT ACGTGTGCCA GTTTCCAGAC CAGGAGGAAG TGCGTCTCTT CTTTCCGCTG
CACCTCTGGG TGAAGAATGT GTTCCTAAAC CAGACTCGGA CTCAGCGAGT CCTCTTTGTG
GACAGTGTAG GCCTGCCGGC TCCCCCCAGT ATCATCAAGG CCATGGGTGG GAGCCAGCCA
GGGGAACTTC AGATCAGCTG GGAGGAGCCA GCTCCAGAAA TCAGTGATTT CCTGAGGTAC
GAACTCCGCT ATGGCCCCAG AGATCCCAAG AACTCCACTG GTCCCACGGT CATACAGCTG
ATTGCCACAG AAACCTGCTG CCCTGCTCTG CAGAGGCCTC ACTCAGCCTC TGCTCTGGAC
CAGTCTCCAT GTGCTCAGCC CACAATGCCC TGGCAAGATG GACCAAAGCA GACCTCCCCA
AGTAGAGAAG CTTCAGCTCT GACAGCAGAG GGTGGAAGCT GCCTCATCTC AGGACTCCAG
CCTGGCAACT CCTACTGGCT GCAGCTGCGC AGCGAACCTG ATGGGATCTC CCTCGGTGGC
TCCTGGGGAT CCTGGTCCCT CCCTGTGACT GTGGACCTGC CTGGAGATGC AGTGGCACTT
GGACTGCAAT GCTTTACCTT GGACCTGAAG AATGTTACCT GTCAATGGCA GCAACAGGAC
CATGCTAGCT CCCAAGGCTT CTTCTACCAC AGCAGGGCAC GGTGCTGCCC CAGAGACAGG
TACCCCATCT GGGAGAACTG CGAAGAGGAA GAGAAAACAA ATCCAGGACT ACAGACCCCA
CAGTTCTCTC GCTGCCACTT CAAGTCACGA AATGACAGCA TTATTCACAT CCTTGTGGAG
GTGACCACAG CCCCGGGTAC TGTTCACAGC TACCTGGGCT CCCCTTTCTG GATCCACCAG
GCTGTGCGCC TCCCCACCCC AAACTTGCAC TGGAGGGAGA TCTCCAGTGG GCATCTGGAA
TTGGAGTGGC AGCACCCATC GTCCTGGGCA GCCCAAGAGA CCTGTTATCA ACTCCGATAC
ACAGGAGAAG GCCATCAGGA CTGGAAGGTG CTGGAGCCGC CTCTCGGGGC CCGAGGAGGG
ACCCTGGAGC TGCGCCCGCG ATCTCGCTAC CGTTTACAGC TGCGCGCCAG GCTCAACGGC
CCCACCTACC AAGGTCCCTG GAGCTCGTGG TCGGACCCAA CTAGGGTGGA GACCGCCACC
GAGACCGCCT GGATCTCCTT GGTGACCGCT CTGCATCTAG TGCTGGGCCT CAGCGCCGTC
CTGGGCCTGC TGCTGCTGAG GTGGCAGTTT CCTGCACACT ACAGGAGACT GAGGCATGCC
CTGTGGCCCT CACTTCCAGA CCTGCACCGG GTCCTAGGCC AGTACCTTAG GGACACTGCA
GCCCTGAGCC CGCCCAAGGC CACAGTCTCA GATACCTGTG AAGAAGTGGA ACCCAGCCTC
CTTGAAATCC TCCCCAAGTC CTCAGAGAGG ACTCCTTTGC CCCTGTGTTC CTCCCAGGCC
CAGATGGACT ACCGAAGATT GCAGCCTTCT TGCCTGGGGA CCATGCCCCT GTCTGTGTGC
CCACCCATGG CTGAGTCAGG GTCCTGCTGT ACCACCCACA TTGCCAACCA TTCCTACCTA
CCACTAAGCT ATTGGCAGCA GCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011539780.1
CDS432..2318
Misc Feature(1)510..794(+)
Misc Feature(2)807..1244(+)
Misc Feature(3)807..1199(+)
Misc Feature(4)1215..1229(+)
Misc Feature(5)1593..1865(+)
Misc Feature(6)1767..1829(+)
Misc Feature(7)1830..1844(+)
Translation
Position Chain Variation Link
27 27 c, t dbSNP:569867759
28 28 c, t dbSNP:184424095
32 32 a, c dbSNP:839993
71 71 c, t dbSNP:543598312
72 72 a, g dbSNP:112430569
79 79 c, t dbSNP:118091889
88 88 a, c dbSNP:189353354
92 92 c, g dbSNP:537920305
104 104 a, g dbSNP:181724591
112 112 g, t dbSNP:759072811
134 134 a, g dbSNP:710252
169 169 a, t dbSNP:547428179
211 211 g, t dbSNP:184408905
277 277 c, t dbSNP:116691358
325 325 c, t dbSNP:142826341
373 373 c, t dbSNP:111248552
383 383 a, g dbSNP:373338567
388 388 c, g dbSNP:770784327
394 394 a, g dbSNP:570118884
398 398 a, g dbSNP:745769570
400 400 c, t dbSNP:771652216
401 401 a, g dbSNP:774857103
419 419 a, c dbSNP:746384923
424 424 c, t dbSNP:376992297
425 425 a, g dbSNP:539244587
435 435 c, t dbSNP:763338238
436 436 c, t dbSNP:188798468
441 441 c, t dbSNP:752112087
442 442 a, g dbSNP:755410233
453 453 a, g dbSNP:572208458
477 477 a, g dbSNP:541014818
488 488 a, c dbSNP:752706183
491 491 a, g dbSNP:756165019
503 503 c, t dbSNP:148327872
521 521 c, t dbSNP:142697469
527 527 g, t dbSNP:17292650
530 530 c, t dbSNP:747864027
531 531 c, t dbSNP:769423189
537 537 c, t dbSNP:148434485
538 538 a, g dbSNP:147938568
541 541 c, t dbSNP:772445486
543 543 c, t dbSNP:775885799
544 544 c, t dbSNP:145714475
555 555 a, g dbSNP:764333753
569 569 c, t dbSNP:753950108
572 572 a, g dbSNP:761922496
583 583 c, t dbSNP:6087
584 584 a, g dbSNP:141077413
585 585 c, t dbSNP:543174329
595 595 c, t dbSNP:201727975
597 597 c, t dbSNP:766172846
619 619 c, t dbSNP:61754776
620 620 a, g dbSNP:6086
621 621 a, c dbSNP:780738649
622 622 a, g, t dbSNP:368753117
629 629 a, g dbSNP:748957880
633 633 c, t dbSNP:756918176
634 634 a, g dbSNP:148276667
641 641 c, t dbSNP:747378368
645 645 -, ct dbSNP:587778514
647 647 c, g dbSNP:769101640
660 660 a, g dbSNP:777298237
661 661 c, t dbSNP:748641693
662 662 g, t dbSNP:769803049
664 664 a, c dbSNP:773339670
671 671 g, t dbSNP:150725726
673 673 a, g dbSNP:587778516
678 678 c, t dbSNP:763144679
679 679 a, c, g dbSNP:766638870
683 683 c, t dbSNP:759361904
693 693 c, t dbSNP:767255141
702 702 c, g dbSNP:752682807
705 705 a, g dbSNP:267598614
708 708 a, g dbSNP:760631916
714 714 c, t dbSNP:763568293
715 715 c, g dbSNP:28928907
716 716 c, t dbSNP:140354935
723 723 c, t dbSNP:145313814
727 727 c, g, t dbSNP:750046020
728 728 a, g dbSNP:370001905
735 735 c, t dbSNP:202206935
749 749 c, t dbSNP:142446401
750 750 a, g dbSNP:12731981
764 764 c, g dbSNP:778085169
769 769 a, g dbSNP:749391587
778 778 a, g dbSNP:770877156
780 780 c, g dbSNP:774581988
784 784 c, t dbSNP:759736065
786 786 -, t dbSNP:587778515
792 792 g, t dbSNP:201101813
801 801 c, g dbSNP:775250202
808 808 c, t dbSNP:371227552
809 809 a, g dbSNP:768283382
811 811 c, t dbSNP:375532666
814 814 c, g dbSNP:761623764
817 817 a, c dbSNP:764904424
818 818 a, c, g dbSNP:146579984
821 821 a, t dbSNP:766136562
829 829 a, c dbSNP:751133047
835 835 c, t dbSNP:756524749
838 838 a, g dbSNP:764347305
840 840 a, g dbSNP:567791154
845 845 a, c dbSNP:757759824
855 855 a, g dbSNP:267598615
866 866 c, g dbSNP:779024886
867 867 a, c dbSNP:745949371
870 870 c, t dbSNP:758428763
874 874 a, t dbSNP:780341646
878 878 a, g dbSNP:747175671
888 888 g, t dbSNP:768536266
890 890 a, c dbSNP:150145932
910 910 -, acg dbSNP:758522045
912 912 a, g dbSNP:6088
919 919 a, g dbSNP:769867913
921 921 c, t dbSNP:773219466
924 924 a, g dbSNP:762584016
925 925 g, t dbSNP:765939441
927 927 c, g dbSNP:202137992
931 931 c, g dbSNP:759083048
935 935 c, t dbSNP:764588069
939 939 -, a dbSNP:587778517
945 945 c, t dbSNP:746331868
953 953 c, t dbSNP:17572791
958 958 c, t dbSNP:137952228
959 959 a, g dbSNP:147130173
966 966 a, c, g, t dbSNP:121913610
968 968 a, g dbSNP:779966701
969 969 a, c, g dbSNP:747322395
973 973 c, t dbSNP:781416266
989 989 c, t dbSNP:747962998
992 992 a, c dbSNP:769674316
997 997 a, c dbSNP:749596497
1003 1003 a, g dbSNP:201050642
1006 1006 a, g dbSNP:770518208
1017 1017 a, g dbSNP:370346540
1029 1029 a, g dbSNP:201385967
1032 1032 a, c dbSNP:111460954
1042 1042 a, g dbSNP:771639707
1051 1051 c, t dbSNP:775165199
1052 1052 c, t dbSNP:762183185
1065 1065 c, g dbSNP:142737453
1084 1084 c, t dbSNP:750968115
1087 1087 a, c dbSNP:141063159
1094 1094 a, t dbSNP:766536759
1100 1100 a, g dbSNP:16830693
1106 1106 a, g dbSNP:767614158
1114 1114 a, c dbSNP:753009936
1119 1119 a, g dbSNP:765133601
1122 1122 g, t dbSNP:144210383
1129 1129 g, t dbSNP:752686531
1140 1140 c, t dbSNP:368330272
1143 1143 a, g dbSNP:587778519
1152 1152 -, ctgg dbSNP:587778518
1160 1160 c, t dbSNP:753703546
1161 1161 c, t dbSNP:757014193
1164 1164 c, t dbSNP:141311765
1167 1167 c, t dbSNP:745790969
1173 1173 c, g dbSNP:771519257
1179 1179 c, t dbSNP:121913611
1184 1184 c, t dbSNP:746721066
1185 1185 a, g dbSNP:528834914
1192 1192 a, t dbSNP:776198457
1193 1193 a, t dbSNP:749648068
1203 1203 c, t dbSNP:117656396
1205 1205 c, t dbSNP:774771300
1206 1206 a, g dbSNP:367877736
1225 1225 c, g dbSNP:760059307
1233 1233 a, c dbSNP:28928908
1236 1236 c, g dbSNP:767775230
1266 1266 a, g dbSNP:200023952
1294 1294 a, g dbSNP:113696793
1295 1295 c, t dbSNP:772722404
1325 1325 a, t dbSNP:776078015
1326 1326 c, g, t dbSNP:751677429
1331 1331 a, c dbSNP:764243911
1332 1332 a, c dbSNP:200993480
1342 1342 -, cca dbSNP:748600815
1342 1342 c, t dbSNP:776753502
1348 1348 g, t dbSNP:762088430
1350 1350 g, t dbSNP:200502271
1353 1353 a, t dbSNP:372164360
1354 1354 g, t dbSNP:750244518
1359 1359 c, t dbSNP:758121875
1363 1363 a, g, t dbSNP:765489603
1371 1371 c, t dbSNP:566429212
1372 1372 a, g dbSNP:149265851
1373 1373 g, t dbSNP:138636505
1379 1379 -, c dbSNP:770457041
1380 1380 c, t dbSNP:755781624
1381 1381 a, c dbSNP:777235976
1384 1384 a, g dbSNP:555380509
1386 1386 a, g dbSNP:772528368
1388 1388 c, t dbSNP:202119310
1393 1393 a, g dbSNP:781482698
1397 1397 c, t dbSNP:144491350
1400 1400 c, t dbSNP:769712782
1404 1404 a, g dbSNP:369486165
1412 1412 c, t dbSNP:141526418
1413 1413 a, g dbSNP:139770726
1419 1419 a, g dbSNP:774120606
1422 1422 c, g dbSNP:759271926
1424 1424 a, g dbSNP:767497390
1433 1433 c, t dbSNP:554597610
1448 1448 c, t dbSNP:779743755
1450 1450 a, c, t dbSNP:61769040
1453 1453 a, g dbSNP:146071368
1461 1461 c, t dbSNP:201998783
1463 1463 c, t dbSNP:753608517
1468 1468 a, g dbSNP:756953598
1473 1473 a, g dbSNP:546510242
1479 1479 c, t dbSNP:751975712
1512 1512 g, t dbSNP:149810307
1526 1526 a, g dbSNP:781733134
1529 1529 c, t dbSNP:748581599
1530 1530 ac, gg dbSNP:587778520
1530 1530 a, g dbSNP:190983971
1543 1543 a, c dbSNP:777711726
1544 1544 c, t dbSNP:749571171
1548 1548 a, g dbSNP:771156950
1549 1549 a, g dbSNP:758197487
1550 1550 c, g dbSNP:745697567
1552 1552 -, c dbSNP:759135440
1564 1564 c, t dbSNP:771719278
1578 1578 c, t dbSNP:373621350
1583 1583 c, t dbSNP:376638782
1586 1586 c, t dbSNP:757289947
1588 1588 c, t dbSNP:779132175
1589 1589 c, t dbSNP:746059682
1591 1591 a, c dbSNP:771965235
1595 1595 c, t dbSNP:775130885
1600 1600 a, g dbSNP:746757614
1607 1607 a, g dbSNP:148945299
1609 1609 a, c dbSNP:776668472
1614 1614 c, t dbSNP:147608148
1644 1644 c, g dbSNP:764816457
1651 1651 c, t dbSNP:772102737
1652 1652 a, g dbSNP:544064034
1662 1662 a, g dbSNP:371232858
1668 1668 c, g dbSNP:373326579
1676 1676 c, t dbSNP:34928544
1686 1686 c, t dbSNP:753018650
1687 1687 a, g dbSNP:200454070
1693 1693 c, t dbSNP:563996763
1710 1710 a, g dbSNP:754296556
1712 1712 a, c dbSNP:757381016
1716 1716 a, g dbSNP:777906915
1739 1739 a, g dbSNP:191392918
1746 1746 a, g dbSNP:561818288
1747 1747 a, g dbSNP:561724539
1748 1748 a, g dbSNP:530475779
1749 1749 c, g dbSNP:550268500
1756 1756 a, t dbSNP:765671565
1761 1761 c, t dbSNP:368378328
1768 1768 c, t dbSNP:750469665
1809 1809 a, g dbSNP:146120160
1824 1824 a, g, t dbSNP:780160063
1832 1832 a, g dbSNP:754859909
1842 1842 a, t dbSNP:781129632
1845 1845 a, g dbSNP:748155064
1853 1853 c, t dbSNP:769867816
1859 1859 -, g dbSNP:761686103
1866 1866 a, g dbSNP:777700319
1869 1869 a, g dbSNP:748782091
1870 1870 c, t dbSNP:770428240
1876 1876 c, t dbSNP:773927786
1877 1877 c, t dbSNP:759161511
1881 1881 c, t dbSNP:775078555
1883 1883 a, g dbSNP:121913613
1898 1898 c, t dbSNP:140091584
1901 1901 c, t dbSNP:374982038
1914 1914 c, g dbSNP:770166362
1915 1915 g, t dbSNP:773641973
1924 1924 a, g dbSNP:121913614
1926 1926 a, g dbSNP:763504517
1928 1928 c, t dbSNP:766435865
1931 1931 c, t dbSNP:774492794
1946 1946 a, g dbSNP:137990502
1952 1952 a, g dbSNP:367960803
1953 1953 aa, tg dbSNP:121913616
1954 1954 g, t dbSNP:121913615
1964 1964 g, t dbSNP:756010427
1972 1972 a, g dbSNP:763924501
1980 1980 c, t dbSNP:146372205
1983 1983 -, a dbSNP:751744547
1983 1983 a, c dbSNP:143100182
1984 1984 g, t dbSNP:772333479
1999 1999 c, t dbSNP:775704066
2015 2015 g, t dbSNP:371286903
2016 2016 c, t dbSNP:768949299
2019 2019 c, t dbSNP:148784027
2020 2020 a, g dbSNP:3820551
2022 2022 c, g dbSNP:374829965
2028 2028 a, g dbSNP:750421230
2031 2031 c, t dbSNP:369156948
2032 2032 a, g dbSNP:766238875
2039 2039 c, t dbSNP:150816330
2041 2041 c, g dbSNP:372747889
2042 2042 a, g dbSNP:780927987
2044 2044 a, g dbSNP:369504259
2045 2045 c, t dbSNP:374221815
2049 2049 a, g dbSNP:752258550
2050 2050 c, t dbSNP:572129852
2053 2053 c, t dbSNP:779404347
2055 2055 c, g dbSNP:746440317
2056 2056 c, t dbSNP:772706335
2060 2060 c, t dbSNP:377715574
2061 2061 c, t dbSNP:747148270
2063 2063 a, g dbSNP:200328093
2063 2063 -, g dbSNP:755257605
2065 2065 a, c dbSNP:755145836
2071 2071 c, t dbSNP:781129953
2076 2076 g, t dbSNP:150004498
2079 2079 g, t dbSNP:142161843
2090 2090 g, t dbSNP:368615234
2093 2093 a, g dbSNP:202022473
2111 2111 c, t dbSNP:749111958
2122 2122 a, t dbSNP:770680742
2129 2129 c, g dbSNP:774393112
2149 2149 c, t dbSNP:147851301
2151 2151 c, t dbSNP:767119189
2154 2154 -, ct dbSNP:770402221
2155 2155 c, t dbSNP:587778513
2158 2158 g, t dbSNP:760201154
2170 2170 c, t dbSNP:763874100
2174 2174 a, g dbSNP:753514496
2176 2176 c, t dbSNP:758886118
2181 2181 c, t dbSNP:766642690
2183 2183 c, t dbSNP:752177268
2184 2184 c, t dbSNP:755447085
2185 2185 a, g dbSNP:200500828
2188 2188 a, g, t dbSNP:781682729
2204 2204 c, t dbSNP:143457144
2213 2213 c, t dbSNP:140794721
2214 2214 a, g dbSNP:749487517
2215 2215 c, t dbSNP:770770219
2219 2219 a, c dbSNP:374553815
2225 2225 c, t dbSNP:778565223
2226 2226 a, g dbSNP:758351725
2235 2235 c, t dbSNP:745606938
2236 2236 a, c dbSNP:771913364
2251 2251 c, g, t dbSNP:138450235
2252 2252 g, t dbSNP:768263859
2259 2259 a, t dbSNP:776486204
2260 2260 c, g dbSNP:201475315
2270 2270 c, g dbSNP:764878611
2283 2283 g, t dbSNP:367594982
2287 2287 a, c dbSNP:751884662
2292 2292 c, t dbSNP:759895996
2304 2304 c, t dbSNP:536317440
2306 2306 c, g dbSNP:753170989
2314 2314 c, t dbSNP:121913612
2318 2318 a, g dbSNP:756235478
2325 2325 a, g dbSNP:556314326
2333 2333 c, t dbSNP:371858774
2334 2334 g, t dbSNP:375202792
2336 2336 c, t dbSNP:569647683
2344 2344 c, t dbSNP:779134942
2346 2346 c, g dbSNP:745669443
2349 2349 c, t dbSNP:538671145
2352 2352 a, g dbSNP:779909585
2364 2364 -, gagact dbSNP:778468275
2364 2364 a, g dbSNP:369565019
2404 2404 c, t dbSNP:777930147
2412 2412 a, c dbSNP:77858532
2429 2429 c, g dbSNP:186807040
2434 2434 g, t dbSNP:554711682
2437 2437 a, g dbSNP:771532230
2488 2488 a, g dbSNP:574563778
2526 2526 c, t dbSNP:781777434
2589 2589 c, t dbSNP:144331455
2598 2598 c, t dbSNP:555958797
2611 2611 -, t dbSNP:568253162
2619 2619 c, t dbSNP:753950232
2682 2682 c, t dbSNP:138892634
2718 2718 c, t dbSNP:558390922
2798 2798 c, g dbSNP:773809357
2820 2820 c, t dbSNP:575782596
2855 2855 c, t dbSNP:535370572
2866 2866 a, c dbSNP:765780152
2873 2873 a, g dbSNP:761545265
2888 2888 c, t dbSNP:771437539
2889 2889 a, g dbSNP:772813059
2891 2891 a, g dbSNP:192016153
2896 2896 c, t dbSNP:565438629

Target ORF information:

RefSeq Version XM_011541478
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens MPL proto-oncogene, thrombopoietin receptor (MPL), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011541478

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCCCTCCT GGGCCCTCTT CATGGTCACC TCCTGCCTCC TCCTGGCCCC TCAAAACCTG 
GCCCAAGTCA GCAGCCAAGA CTCAGAGCCC CTGAAGTGTT TCTCCCGAAC ATTTGAGGAC
CTCACTTGCT TCTGGGATGA GGAAGAGGCA GCGCCCAGTG GGACATACCA GCTGCTGTAT
GCCTACCCGC GGGAGAAGCC CCGTGCTTGC CCCCTGAGTT CCCAGAGCAT GCCCCACTTT
GGAACCCGAT ACGTGTGCCA GTTTCCAGAC CAGGAGGAAG TGCGTCTCTT CTTTCCGCTG
CACCTCTGGG TGAAGAATGT GTTCCTAAAC CAGACTCGGA CTCAGCGAGT CCTCTTTGTG
GACAGTGTAG GCCTGCCGGC TCCCCCCAGT ATCATCAAGG CCATGGGTGG GAGCCAGCCA
GGGGAACTTC AGATCAGCTG GGAGGAGCCA GCTCCAGAAA TCAGTGATTT CCTGAGGTAC
GAACTCCGCT ATGGCCCCAG AGATCCCAAG AACTCCACTG GTCCCACGGT CATACAGCTG
ATTGCCACAG AAACCTGCTG CCCTGCTCTG CAGAGGCCTC ACTCAGCCTC TGCTCTGGAC
CAGTCTCCAT GTGCTCAGCC CACAATGCCC TGGCAAGATG GACCAAAGCA GACCTCCCCA
AGTAGAGAAG CTTCAGCTCT GACAGCAGAG GGTGGAAGCT GCCTCATCTC AGGACTCCAG
CCTGGCAACT CCTACTGGCT GCAGCTGCGC AGCGAACCTG ATGGGATCTC CCTCGGTGGC
TCCTGGGGAT CCTGGTCCCT CCCTGTGACT GTGGACCTGC CTGGAGATGC AGTGGCACTT
GGACTGCAAT GCTTTACCTT GGACCTGAAG AATGTTACCT GTCAATGGCA GCAACAGGAC
CATGCTAGCT CCCAAGGCTT CTTCTACCAC AGCAGGGCAC GGTGCTGCCC CAGAGACAGG
TACCCCATCT GGGAGAACTG CGAAGAGGAA GAGAAAACAA ATCCAGGACT ACAGACCCCA
CAGTTCTCTC GCTGCCACTT CAAGTCACGA AATGACAGCA TTATTCACAT CCTTGTGGAG
GTGACCACAG CCCCGGGTAC TGTTCACAGC TACCTGGGCT CCCCTTTCTG GATCCACCAG
GCTGTGCGCC TCCCCACCCC AAACTTGCAC TGGAGGGAGA TCTCCAGTGG GCATCTGGAA
TTGGAGTGGC AGCACCCATC GTCCTGGGCA GCCCAAGAGA CCTGTTATCA ACTCCGATAC
ACAGGAGAAG GCCATCAGGA CTGGAAGGTG CTGGAGCCGC CTCTCGGGGC CCGAGGAGGG
ACCCTGGAGC TGCGCCCGCG ATCTCGCTAC CGTTTACAGC TGCGCGCCAG GCTCAACGGC
CCCACCTACC AAGGTCCCTG GAGCTCGTGG TCGGACCCAA CTAGGGTGGA GACCGCCACC
GAGACCGCCT GGATCTCCTT GGTGACCGCT CTGCATCTAG TGCTGGGCCT CAGCGCCGTC
CTGGGCCTGC TGCTGCTGAG GTGGCAGTTT CCTGCACACT ACAGGAGACT GAGGCATGCC
CTGTGGCCCT CACTTCCAGA CCTGCACCGG GTCCTAGGCC AGTACCTTAG GGACACTGCA
GCCCTGAGCC CGCCCAAGGC CACAGTCTCA GATACCTGTG AAGAAGTGGA ACCCAGCCTC
CTTGAAATCC TCCCCAAGTC CTCAGAGAGG ACTCCTTTGC CCCTGTGTTC CTCCCAGGCC
CAGATGGACT ACCGAAGATT GCAGCCTTCT TGCCTGGGGA CCATGCCCCT GTCTGTGTGC
CCACCCATGG CTGAGTCAGG GTCCTGCTGT ACCACCCACA TTGCCAACCA TTCCTACCTA
CCACTAAGCT ATTGGCAGCA GCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28553
Accession Version NM_005373.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1908bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product thrombopoietin receptor precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139289.6 and M90102.1. This sequence is a reference standard in the RefSeqGene project. On Apr 4, 2008 this sequence version replaced gi:4885490. Summary: In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. [provided by RefSeq, Jul 2008]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M90102.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCCCTCCT GGGCCCTCTT CATGGTCACC TCCTGCCTCC TCCTGGCCCC TCAAAACCTG 
GCCCAAGTCA GCAGCCAAGA TGTCTCCTTG CTGGCATCAG ACTCAGAGCC CCTGAAGTGT
TTCTCCCGAA CATTTGAGGA CCTCACTTGC TTCTGGGATG AGGAAGAGGC AGCGCCCAGT
GGGACATACC AGCTGCTGTA TGCCTACCCG CGGGAGAAGC CCCGTGCTTG CCCCCTGAGT
TCCCAGAGCA TGCCCCACTT TGGAACCCGA TACGTGTGCC AGTTTCCAGA CCAGGAGGAA
GTGCGTCTCT TCTTTCCGCT GCACCTCTGG GTGAAGAATG TGTTCCTAAA CCAGACTCGG
ACTCAGCGAG TCCTCTTTGT GGACAGTGTA GGCCTGCCGG CTCCCCCCAG TATCATCAAG
GCCATGGGTG GGAGCCAGCC AGGGGAACTT CAGATCAGCT GGGAGGAGCC AGCTCCAGAA
ATCAGTGATT TCCTGAGGTA CGAACTCCGC TATGGCCCCA GAGATCCCAA GAACTCCACT
GGTCCCACGG TCATACAGCT GATTGCCACA GAAACCTGCT GCCCTGCTCT GCAGAGGCCT
CACTCAGCCT CTGCTCTGGA CCAGTCTCCA TGTGCTCAGC CCACAATGCC CTGGCAAGAT
GGACCAAAGC AGACCTCCCC AAGTAGAGAA GCTTCAGCTC TGACAGCAGA GGGTGGAAGC
TGCCTCATCT CAGGACTCCA GCCTGGCAAC TCCTACTGGC TGCAGCTGCG CAGCGAACCT
GATGGGATCT CCCTCGGTGG CTCCTGGGGA TCCTGGTCCC TCCCTGTGAC TGTGGACCTG
CCTGGAGATG CAGTGGCACT TGGACTGCAA TGCTTTACCT TGGACCTGAA GAATGTTACC
TGTCAATGGC AGCAACAGGA CCATGCTAGC TCCCAAGGCT TCTTCTACCA CAGCAGGGCA
CGGTGCTGCC CCAGAGACAG GTACCCCATC TGGGAGAACT GCGAAGAGGA AGAGAAAACA
AATCCAGGAC TACAGACCCC ACAGTTCTCT CGCTGCCACT TCAAGTCACG AAATGACAGC
ATTATTCACA TCCTTGTGGA GGTGACCACA GCCCCGGGTA CTGTTCACAG CTACCTGGGC
TCCCCTTTCT GGATCCACCA GGCTGTGCGC CTCCCCACCC CAAACTTGCA CTGGAGGGAG
ATCTCCAGTG GGCATCTGGA ATTGGAGTGG CAGCACCCAT CGTCCTGGGC AGCCCAAGAG
ACCTGTTATC AACTCCGATA CACAGGAGAA GGCCATCAGG ACTGGAAGGT GCTGGAGCCG
CCTCTCGGGG CCCGAGGAGG GACCCTGGAG CTGCGCCCGC GATCTCGCTA CCGTTTACAG
CTGCGCGCCA GGCTCAACGG CCCCACCTAC CAAGGTCCCT GGAGCTCGTG GTCGGACCCA
ACTAGGGTGG AGACCGCCAC CGAGACCGCC TGGATCTCCT TGGTGACCGC TCTGCATCTA
GTGCTGGGCC TCAGCGCCGT CCTGGGCCTG CTGCTGCTGA GGTGGCAGTT TCCTGCACAC
TACAGGAGAC TGAGGCATGC CCTGTGGCCC TCACTTCCAG ACCTGCACCG GGTCCTAGGC
CAGTACCTTA GGGACACTGC AGCCCTGAGC CCGCCCAAGG CCACAGTCTC AGATACCTGT
GAAGAAGTGG AACCCAGCCT CCTTGAAATC CTCCCCAAGT CCTCAGAGAG GACTCCTTTG
CCCCTGTGTT CCTCCCAGGC CCAGATGGAC TACCGAAGAT TGCAGCCTTC TTGCCTGGGG
ACCATGCCCC TGTCTGTGTG CCCACCCATG GCTGAGTCAG GGTCCTGCTG TACCACCCAC
ATTGCCAACC ATTCCTACCT ACCACTAAGC TATTGGCAGC AGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_005364.1
CDS46..1953
Misc Feature(1)19..21(+)
Misc Feature(2)118..429(+)
Misc Feature(3)442..879(+)
Misc Feature(4)442..834(+)
Misc Feature(5)850..864(+)
Misc Feature(6)1228..1500(+)
Misc Feature(7)1402..1464(+)
Misc Feature(8)1465..1479(+)
Misc Feature(9)1465..1479(+)
Misc Feature(10)1519..1584(+)
Misc Feature(11)1627..1653(+)
Misc Feature(12)1921..1923(+)
Misc Feature(13)1936..1938(+)
Exon (1)1..124
Gene:MPL
Gene Synonym:
Exon (2)125..257
Gene:MPL
Gene Synonym:
Exon (3)258..436
Gene:MPL
Gene Synonym:
Exon (4)437..735
Gene:MPL
Gene Synonym:
Exon (5)736..898
Gene:MPL
Gene Synonym:
Exon (6)899..1025
Gene:MPL
Gene Synonym:
Exon (7)1026..1210
Gene:MPL
Gene Synonym:
Exon (8)1211..1353
Gene:MPL
Gene Synonym:
Exon (9)1354..1513
Gene:MPL
Gene Synonym:
Exon (10)1514..1610
Gene:MPL
Gene Synonym:
Exon (11)1611..1698
Gene:MPL
Gene Synonym:
Exon (12)1699..3645
Gene:MPL
Gene Synonym:
Translation
Position Chain Variation Link
2 2 c, g dbSNP:770784327
8 8 a, g dbSNP:570118884
12 12 a, g dbSNP:745769570
14 14 c, t dbSNP:771652216
15 15 a, g dbSNP:774857103
33 33 a, c dbSNP:746384923
38 38 c, t dbSNP:376992297
39 39 a, g dbSNP:539244587
49 49 c, t dbSNP:763338238
50 50 c, t dbSNP:188798468
55 55 c, t dbSNP:752112087
56 56 a, g dbSNP:755410233
67 67 a, g dbSNP:572208458
91 91 a, g dbSNP:541014818
102 102 a, c dbSNP:752706183
105 105 a, g dbSNP:756165019
117 117 c, t dbSNP:148327872
128 128 -, tc dbSNP:752416111
139 139 a, g dbSNP:746914831
156 156 c, t dbSNP:142697469
162 162 g, t dbSNP:17292650
165 165 c, t dbSNP:747864027
166 166 c, t dbSNP:769423189
172 172 c, t dbSNP:148434485
173 173 a, g dbSNP:147938568
176 176 c, t dbSNP:772445486
178 178 c, t dbSNP:775885799
179 179 c, t dbSNP:145714475
190 190 a, g dbSNP:764333753
204 204 c, t dbSNP:753950108
207 207 a, g dbSNP:761922496
218 218 c, t dbSNP:6087
219 219 a, g dbSNP:141077413
220 220 c, t dbSNP:543174329
230 230 c, t dbSNP:201727975
232 232 c, t dbSNP:766172846
254 254 c, t dbSNP:61754776
255 255 a, g dbSNP:6086
256 256 a, c dbSNP:780738649
257 257 a, g, t dbSNP:368753117
264 264 a, g dbSNP:748957880
268 268 c, t dbSNP:756918176
269 269 a, g dbSNP:148276667
276 276 c, t dbSNP:747378368
280 280 -, ct dbSNP:587778514
282 282 c, g dbSNP:769101640
295 295 a, g dbSNP:777298237
296 296 c, t dbSNP:748641693
297 297 g, t dbSNP:769803049
299 299 a, c dbSNP:773339670
306 306 g, t dbSNP:150725726
308 308 a, g dbSNP:587778516
313 313 c, t dbSNP:763144679
314 314 a, c, g dbSNP:766638870
318 318 c, t dbSNP:759361904
328 328 c, t dbSNP:767255141
337 337 c, g dbSNP:752682807
340 340 a, g dbSNP:267598614
343 343 a, g dbSNP:760631916
349 349 c, t dbSNP:763568293
350 350 c, g dbSNP:28928907
351 351 c, t dbSNP:140354935
358 358 c, t dbSNP:145313814
362 362 c, g, t dbSNP:750046020
363 363 a, g dbSNP:370001905
370 370 c, t dbSNP:202206935
384 384 c, t dbSNP:142446401
385 385 a, g dbSNP:12731981
399 399 c, g dbSNP:778085169
404 404 a, g dbSNP:749391587
413 413 a, g dbSNP:770877156
415 415 c, g dbSNP:774581988
419 419 c, t dbSNP:759736065
421 421 -, t dbSNP:587778515
427 427 g, t dbSNP:201101813
436 436 c, g dbSNP:775250202
443 443 c, t dbSNP:371227552
444 444 a, g dbSNP:768283382
446 446 c, t dbSNP:375532666
449 449 c, g dbSNP:761623764
452 452 a, c dbSNP:764904424
453 453 a, c, g dbSNP:146579984
456 456 a, t dbSNP:766136562
464 464 a, c dbSNP:751133047
470 470 c, t dbSNP:756524749
473 473 a, g dbSNP:764347305
475 475 a, g dbSNP:567791154
480 480 a, c dbSNP:757759824
490 490 a, g dbSNP:267598615
501 501 c, g dbSNP:779024886
502 502 a, c dbSNP:745949371
505 505 c, t dbSNP:758428763
509 509 a, t dbSNP:780341646
513 513 a, g dbSNP:747175671
523 523 g, t dbSNP:768536266
525 525 a, c dbSNP:150145932
545 545 -, acg dbSNP:758522045
547 547 a, g dbSNP:6088
554 554 a, g dbSNP:769867913
556 556 c, t dbSNP:773219466
559 559 a, g dbSNP:762584016
560 560 g, t dbSNP:765939441
562 562 c, g dbSNP:202137992
566 566 c, g dbSNP:759083048
570 570 c, t dbSNP:764588069
574 574 -, a dbSNP:587778517
580 580 c, t dbSNP:746331868
588 588 c, t dbSNP:17572791
593 593 c, t dbSNP:137952228
594 594 a, g dbSNP:147130173
601 601 a, c, g, t dbSNP:121913610
603 603 a, g dbSNP:779966701
604 604 a, c, g dbSNP:747322395
608 608 c, t dbSNP:781416266
624 624 c, t dbSNP:747962998
627 627 a, c dbSNP:769674316
632 632 a, c dbSNP:749596497
638 638 a, g dbSNP:201050642
641 641 a, g dbSNP:770518208
652 652 a, g dbSNP:370346540
664 664 a, g dbSNP:201385967
667 667 a, c dbSNP:111460954
677 677 a, g dbSNP:771639707
686 686 c, t dbSNP:775165199
687 687 c, t dbSNP:762183185
700 700 c, g dbSNP:142737453
719 719 c, t dbSNP:750968115
722 722 a, c dbSNP:141063159
729 729 a, t dbSNP:766536759
735 735 a, g dbSNP:16830693
741 741 a, g dbSNP:767614158
749 749 a, c dbSNP:753009936
754 754 a, g dbSNP:765133601
757 757 g, t dbSNP:144210383
764 764 g, t dbSNP:752686531
775 775 c, t dbSNP:368330272
778 778 a, g dbSNP:587778519
787 787 -, ctgg dbSNP:587778518
795 795 c, t dbSNP:753703546
796 796 c, t dbSNP:757014193
799 799 c, t dbSNP:141311765
802 802 c, t dbSNP:745790969
808 808 c, g dbSNP:771519257
814 814 c, t dbSNP:121913611
819 819 c, t dbSNP:746721066
820 820 a, g dbSNP:528834914
827 827 a, t dbSNP:776198457
828 828 a, t dbSNP:749648068
838 838 c, t dbSNP:117656396
840 840 c, t dbSNP:774771300
841 841 a, g dbSNP:367877736
860 860 c, g dbSNP:760059307
868 868 a, c dbSNP:28928908
871 871 c, g dbSNP:767775230
901 901 a, g dbSNP:200023952
929 929 a, g dbSNP:113696793
930 930 c, t dbSNP:772722404
960 960 a, t dbSNP:776078015
961 961 c, g, t dbSNP:751677429
966 966 a, c dbSNP:764243911
967 967 a, c dbSNP:200993480
977 977 -, cca dbSNP:748600815
977 977 c, t dbSNP:776753502
983 983 g, t dbSNP:762088430
985 985 g, t dbSNP:200502271
988 988 a, t dbSNP:372164360
989 989 g, t dbSNP:750244518
994 994 c, t dbSNP:758121875
998 998 a, g, t dbSNP:765489603
1006 1006 c, t dbSNP:566429212
1007 1007 a, g dbSNP:149265851
1008 1008 g, t dbSNP:138636505
1014 1014 -, c dbSNP:770457041
1015 1015 c, t dbSNP:755781624
1016 1016 a, c dbSNP:777235976
1019 1019 a, g dbSNP:555380509
1021 1021 a, g dbSNP:772528368
1023 1023 c, t dbSNP:202119310
1028 1028 a, g dbSNP:781482698
1032 1032 c, t dbSNP:144491350
1035 1035 c, t dbSNP:769712782
1039 1039 a, g dbSNP:369486165
1047 1047 c, t dbSNP:141526418
1048 1048 a, g dbSNP:139770726
1054 1054 a, g dbSNP:774120606
1057 1057 c, g dbSNP:759271926
1059 1059 a, g dbSNP:767497390
1068 1068 c, t dbSNP:554597610
1083 1083 c, t dbSNP:779743755
1085 1085 a, c, t dbSNP:61769040
1088 1088 a, g dbSNP:146071368
1096 1096 c, t dbSNP:201998783
1098 1098 c, t dbSNP:753608517
1103 1103 a, g dbSNP:756953598
1108 1108 a, g dbSNP:546510242
1114 1114 c, t dbSNP:751975712
1147 1147 g, t dbSNP:149810307
1161 1161 a, g dbSNP:781733134
1164 1164 c, t dbSNP:748581599
1165 1165 ac, gg dbSNP:587778520
1165 1165 a, g dbSNP:190983971
1178 1178 a, c dbSNP:777711726
1179 1179 c, t dbSNP:749571171
1183 1183 a, g dbSNP:771156950
1184 1184 a, g dbSNP:758197487
1185 1185 c, g dbSNP:745697567
1187 1187 -, c dbSNP:759135440
1199 1199 c, t dbSNP:771719278
1213 1213 c, t dbSNP:373621350
1218 1218 c, t dbSNP:376638782
1221 1221 c, t dbSNP:757289947
1223 1223 c, t dbSNP:779132175
1224 1224 c, t dbSNP:746059682
1226 1226 a, c dbSNP:771965235
1230 1230 c, t dbSNP:775130885
1235 1235 a, g dbSNP:746757614
1242 1242 a, g dbSNP:148945299
1244 1244 a, c dbSNP:776668472
1249 1249 c, t dbSNP:147608148
1279 1279 c, g dbSNP:764816457
1286 1286 c, t dbSNP:772102737
1287 1287 a, g dbSNP:544064034
1297 1297 a, g dbSNP:371232858
1303 1303 c, g dbSNP:373326579
1311 1311 c, t dbSNP:34928544
1321 1321 c, t dbSNP:753018650
1322 1322 a, g dbSNP:200454070
1328 1328 c, t dbSNP:563996763
1345 1345 a, g dbSNP:754296556
1347 1347 a, c dbSNP:757381016
1351 1351 a, g dbSNP:777906915
1374 1374 a, g dbSNP:191392918
1381 1381 a, g dbSNP:561818288
1382 1382 a, g dbSNP:561724539
1383 1383 a, g dbSNP:530475779
1384 1384 c, g dbSNP:550268500
1391 1391 a, t dbSNP:765671565
1396 1396 c, t dbSNP:368378328
1403 1403 c, t dbSNP:750469665
1444 1444 a, g dbSNP:146120160
1459 1459 a, g, t dbSNP:780160063
1467 1467 a, g dbSNP:754859909
1477 1477 a, t dbSNP:781129632
1480 1480 a, g dbSNP:748155064
1488 1488 c, t dbSNP:769867816
1494 1494 -, g dbSNP:761686103
1501 1501 a, g dbSNP:777700319
1504 1504 a, g dbSNP:748782091
1505 1505 c, t dbSNP:770428240
1511 1511 c, t dbSNP:773927786
1512 1512 c, t dbSNP:759161511
1516 1516 c, t dbSNP:775078555
1518 1518 a, g dbSNP:121913613
1533 1533 c, t dbSNP:140091584
1536 1536 c, t dbSNP:374982038
1549 1549 c, g dbSNP:770166362
1550 1550 g, t dbSNP:773641973
1559 1559 a, g dbSNP:121913614
1561 1561 a, g dbSNP:763504517
1563 1563 c, t dbSNP:766435865
1566 1566 c, t dbSNP:774492794
1581 1581 a, g dbSNP:137990502
1587 1587 a, g dbSNP:367960803
1588 1588 aa, tg dbSNP:121913616
1589 1589 g, t dbSNP:121913615
1599 1599 g, t dbSNP:756010427
1607 1607 a, g dbSNP:763924501
1615 1615 c, t dbSNP:146372205
1618 1618 -, a dbSNP:751744547
1618 1618 a, c dbSNP:143100182
1619 1619 g, t dbSNP:772333479
1634 1634 c, t dbSNP:775704066
1650 1650 g, t dbSNP:371286903
1651 1651 c, t dbSNP:768949299
1654 1654 c, t dbSNP:148784027
1655 1655 a, g dbSNP:3820551
1657 1657 c, g dbSNP:374829965
1663 1663 a, g dbSNP:750421230
1666 1666 c, t dbSNP:369156948
1667 1667 a, g dbSNP:766238875
1674 1674 c, t dbSNP:150816330
1676 1676 c, g dbSNP:372747889
1677 1677 a, g dbSNP:780927987
1679 1679 a, g dbSNP:369504259
1680 1680 c, t dbSNP:374221815
1684 1684 a, g dbSNP:752258550
1685 1685 c, t dbSNP:572129852
1688 1688 c, t dbSNP:779404347
1690 1690 c, g dbSNP:746440317
1691 1691 c, t dbSNP:772706335
1695 1695 c, t dbSNP:377715574
1696 1696 c, t dbSNP:747148270
1698 1698 a, g dbSNP:200328093
1698 1698 -, g dbSNP:755257605
1700 1700 a, c dbSNP:755145836
1706 1706 c, t dbSNP:781129953
1711 1711 g, t dbSNP:150004498
1714 1714 g, t dbSNP:142161843
1725 1725 g, t dbSNP:368615234
1728 1728 a, g dbSNP:202022473
1746 1746 c, t dbSNP:749111958
1757 1757 a, t dbSNP:770680742
1764 1764 c, g dbSNP:774393112
1784 1784 c, t dbSNP:147851301
1786 1786 c, t dbSNP:767119189
1789 1789 -, ct dbSNP:770402221
1790 1790 c, t dbSNP:587778513
1793 1793 g, t dbSNP:760201154
1805 1805 c, t dbSNP:763874100
1809 1809 a, g dbSNP:753514496
1811 1811 c, t dbSNP:758886118
1816 1816 c, t dbSNP:766642690
1818 1818 c, t dbSNP:752177268
1819 1819 c, t dbSNP:755447085
1820 1820 a, g dbSNP:200500828
1823 1823 a, g, t dbSNP:781682729
1839 1839 c, t dbSNP:143457144
1848 1848 c, t dbSNP:140794721
1849 1849 a, g dbSNP:749487517
1850 1850 c, t dbSNP:770770219
1854 1854 a, c dbSNP:374553815
1860 1860 c, t dbSNP:778565223
1861 1861 a, g dbSNP:758351725
1870 1870 c, t dbSNP:745606938
1871 1871 a, c dbSNP:771913364
1886 1886 c, g, t dbSNP:138450235
1887 1887 g, t dbSNP:768263859
1894 1894 a, t dbSNP:776486204
1895 1895 c, g dbSNP:201475315
1905 1905 c, g dbSNP:764878611
1918 1918 g, t dbSNP:367594982
1922 1922 a, c dbSNP:751884662
1927 1927 c, t dbSNP:759895996
1939 1939 c, t dbSNP:536317440
1941 1941 c, g dbSNP:753170989
1949 1949 c, t dbSNP:121913612
1953 1953 a, g dbSNP:756235478
1960 1960 a, g dbSNP:556314326
1968 1968 c, t dbSNP:371858774
1969 1969 g, t dbSNP:375202792
1971 1971 c, t dbSNP:569647683
1979 1979 c, t dbSNP:779134942
1981 1981 c, g dbSNP:745669443
1984 1984 c, t dbSNP:538671145
1987 1987 a, g dbSNP:779909585
1999 1999 -, gagact dbSNP:778468275
1999 1999 a, g dbSNP:369565019
2039 2039 c, t dbSNP:777930147
2047 2047 a, c dbSNP:77858532
2064 2064 c, g dbSNP:186807040
2069 2069 g, t dbSNP:554711682
2072 2072 a, g dbSNP:771532230
2123 2123 a, g dbSNP:574563778
2161 2161 c, t dbSNP:781777434
2224 2224 c, t dbSNP:144331455
2233 2233 c, t dbSNP:555958797
2246 2246 -, t dbSNP:568253162
2254 2254 c, t dbSNP:753950232
2317 2317 c, t dbSNP:138892634
2353 2353 c, t dbSNP:558390922
2433 2433 c, g dbSNP:773809357
2455 2455 c, t dbSNP:575782596
2490 2490 c, t dbSNP:535370572
2501 2501 a, c dbSNP:765780152
2508 2508 a, g dbSNP:761545265
2523 2523 c, t dbSNP:771437539
2524 2524 a, g dbSNP:772813059
2526 2526 a, g dbSNP:192016153
2531 2531 c, t dbSNP:565438629
2554 2554 c, t dbSNP:527941032
2578 2578 c, t dbSNP:182807417
2606 2606 c, t dbSNP:567818153
2615 2615 c, t dbSNP:1763698
2625 2625 c, g, t dbSNP:75425122
2627 2627 a, t dbSNP:77858547
2630 2630 a, t dbSNP:79736278
2636 2636 -, ttttttttttt dbSNP:752218261
2662 2662 c, t dbSNP:185654501
2689 2689 a, g dbSNP:758861942
2701 2701 c, t dbSNP:190071085
2708 2708 c, g dbSNP:555453881
2733 2733 a, g dbSNP:182233357
2742 2742 c, t dbSNP:753837289
2765 2765 a, g dbSNP:759257463
2782 2782 a, t dbSNP:765093709
2791 2791 a, g dbSNP:572052263
2815 2815 a, g dbSNP:558480493
2824 2824 a, g dbSNP:540648773
2835 2835 c, t dbSNP:777894453
2867 2867 c, t dbSNP:186474263
2868 2868 a, g dbSNP:757471629
2882 2882 g, t dbSNP:565546786
2907 2907 c, g dbSNP:755757589
2910 2910 g, t dbSNP:146322472
2923 2923 c, t dbSNP:777372120
2964 2964 a, g dbSNP:577681127
2974 2974 -, t dbSNP:761485378
3007 3007 a, g dbSNP:139528456
3032 3032 -, c dbSNP:35225340
3033 3033 -, aa dbSNP:558674779
3048 3048 c, t dbSNP:781763610
3065 3065 a, g dbSNP:543515941
3066 3066 c, t dbSNP:756380169
3069 3069 a, g dbSNP:144177827
3079 3079 a, g dbSNP:543250497
3115 3115 a, g dbSNP:556230116
3133 3133 a, g dbSNP:117166528
3173 3173 c, t dbSNP:6689207
3175 3175 c, t dbSNP:563079154
3192 3192 a, c dbSNP:527426135
3214 3214 a, g dbSNP:193122067
3225 3225 a, g dbSNP:559905562
3235 3235 g, t dbSNP:771727846
3244 3244 -, c dbSNP:372021668
3244 3244 c, t dbSNP:527705582
3252 3252 a, g dbSNP:375523167
3256 3256 a, t dbSNP:772905876
3282 3282 a, g dbSNP:531904727
3307 3307 a, g dbSNP:746514982
3318 3318 -, a dbSNP:759735488
3324 3324 a, g dbSNP:541688073
3405 3405 c, t dbSNP:139194573
3425 3425 c, t dbSNP:183926045
3439 3439 c, t dbSNP:149940158
3450 3450 g, t dbSNP:570264040
3459 3459 a, g dbSNP:115780311
3472 3472 c, g dbSNP:551999587
3478 3478 c, t dbSNP:144988440
3541 3541 c, t dbSNP:188722111
3556 3556 a, c dbSNP:142139493
3574 3574 c, t dbSNP:562243899
3579 3579 c, t dbSNP:759490775
3613 3613 c, t dbSNP:568055116
3627 3627 a, t dbSNP:536844021

Target ORF information:

RefSeq Version NM_005373
Organism Homo sapiens (human)
Definition Homo sapiens MPL proto-oncogene, thrombopoietin receptor (MPL), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005373

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCCCTCCT GGGCCCTCTT CATGGTCACC TCCTGCCTCC TCCTGGCCCC TCAAAACCTG 
GCCCAAGTCA GCAGCCAAGA TGTCTCCTTG CTGGCATCAG ACTCAGAGCC CCTGAAGTGT
TTCTCCCGAA CATTTGAGGA CCTCACTTGC TTCTGGGATG AGGAAGAGGC AGCGCCCAGT
GGGACATACC AGCTGCTGTA TGCCTACCCG CGGGAGAAGC CCCGTGCTTG CCCCCTGAGT
TCCCAGAGCA TGCCCCACTT TGGAACCCGA TACGTGTGCC AGTTTCCAGA CCAGGAGGAA
GTGCGTCTCT TCTTTCCGCT GCACCTCTGG GTGAAGAATG TGTTCCTAAA CCAGACTCGG
ACTCAGCGAG TCCTCTTTGT GGACAGTGTA GGCCTGCCGG CTCCCCCCAG TATCATCAAG
GCCATGGGTG GGAGCCAGCC AGGGGAACTT CAGATCAGCT GGGAGGAGCC AGCTCCAGAA
ATCAGTGATT TCCTGAGGTA CGAACTCCGC TATGGCCCCA GAGATCCCAA GAACTCCACT
GGTCCCACGG TCATACAGCT GATTGCCACA GAAACCTGCT GCCCTGCTCT GCAGAGGCCT
CACTCAGCCT CTGCTCTGGA CCAGTCTCCA TGTGCTCAGC CCACAATGCC CTGGCAAGAT
GGACCAAAGC AGACCTCCCC AAGTAGAGAA GCTTCAGCTC TGACAGCAGA GGGTGGAAGC
TGCCTCATCT CAGGACTCCA GCCTGGCAAC TCCTACTGGC TGCAGCTGCG CAGCGAACCT
GATGGGATCT CCCTCGGTGG CTCCTGGGGA TCCTGGTCCC TCCCTGTGAC TGTGGACCTG
CCTGGAGATG CAGTGGCACT TGGACTGCAA TGCTTTACCT TGGACCTGAA GAATGTTACC
TGTCAATGGC AGCAACAGGA CCATGCTAGC TCCCAAGGCT TCTTCTACCA CAGCAGGGCA
CGGTGCTGCC CCAGAGACAG GTACCCCATC TGGGAGAACT GCGAAGAGGA AGAGAAAACA
AATCCAGGAC TACAGACCCC ACAGTTCTCT CGCTGCCACT TCAAGTCACG AAATGACAGC
ATTATTCACA TCCTTGTGGA GGTGACCACA GCCCCGGGTA CTGTTCACAG CTACCTGGGC
TCCCCTTTCT GGATCCACCA GGCTGTGCGC CTCCCCACCC CAAACTTGCA CTGGAGGGAG
ATCTCCAGTG GGCATCTGGA ATTGGAGTGG CAGCACCCAT CGTCCTGGGC AGCCCAAGAG
ACCTGTTATC AACTCCGATA CACAGGAGAA GGCCATCAGG ACTGGAAGGT GCTGGAGCCG
CCTCTCGGGG CCCGAGGAGG GACCCTGGAG CTGCGCCCGC GATCTCGCTA CCGTTTACAG
CTGCGCGCCA GGCTCAACGG CCCCACCTAC CAAGGTCCCT GGAGCTCGTG GTCGGACCCA
ACTAGGGTGG AGACCGCCAC CGAGACCGCC TGGATCTCCT TGGTGACCGC TCTGCATCTA
GTGCTGGGCC TCAGCGCCGT CCTGGGCCTG CTGCTGCTGA GGTGGCAGTT TCCTGCACAC
TACAGGAGAC TGAGGCATGC CCTGTGGCCC TCACTTCCAG ACCTGCACCG GGTCCTAGGC
CAGTACCTTA GGGACACTGC AGCCCTGAGC CCGCCCAAGG CCACAGTCTC AGATACCTGT
GAAGAAGTGG AACCCAGCCT CCTTGAAATC CTCCCCAAGT CCTCAGAGAG GACTCCTTTG
CCCCTGTGTT CCTCCCAGGC CCAGATGGAC TACCGAAGAT TGCAGCCTTC TTGCCTGGGG
ACCATGCCCC TGTCTGTGTG CCCACCCATG GCTGAGTCAG GGTCCTGCTG TACCACCCAC
ATTGCCAACC ATTCCTACCT ACCACTAAGC TATTGGCAGC AGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
*
*
*
*