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MSH2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MSH2
Entrez Gene ID 4436
Full Name mutS homolog 2
Synonyms COCA1, FCC1, HNPCC, HNPCC1, LCFS2
General protein information
Preferred Names
DNA mismatch repair protein Msh2
Names
DNA mismatch repair protein Msh2
hMSH2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2p21

Summary This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]. lac of sum
Disorder MIM:

609309

Disorder Html: Colorectal cancer, hereditary nonpolyposis, type 1, 120435 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_001258281 NP_001245210 DNA mismatch repair protein Msh2 isoform 2
XM_005264332 XP_005264389 DNA mismatch repair protein Msh2 isoform X1
XM_011532867 XP_011531169 DNA mismatch repair protein Msh2 isoform X2
NM_000251 NP_000242 DNA mismatch repair protein Msh2 isoform 1



Homo sapiens (human) MSH2 NP_000242.1
Canis lupus familiaris (dog) MSH2 XP_538482.2
Bos taurus (cattle) MSH2 NP_001029756.1
Mus musculus (house mouse) Msh2 NP_032654.1
Rattus norvegicus (Norway rat) Msh2 NP_112320.1
Gallus gallus (chicken) MSH2 XP_426110.4
Danio rerio (zebrafish) msh2 NP_998689.1
Drosophila melanogaster (fruit fly) spel1 NP_001246031.1
Arabidopsis thaliana (thale cress) MSH2 NP_566804.3
Xenopus (Silurana) tropicalis (western clawed frog) msh2 XP_002935427.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MSH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MSH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu16740
NM_001258281 Homo sapiens mutS homolog 2 (MSH2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$339.50-$475.30
$679.00
OHu59053 XM_005264332 PREDICTED: Homo sapiens mutS homolog 2 (MSH2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu59054 XM_011532867 PREDICTED: Homo sapiens mutS homolog 2 (MSH2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu20167
NM_000251 Homo sapiens mutS homolog 2 (MSH2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu16740
Clone ID Related Accession (Same CDS sequence) NM_001258281
Accession Version NM_001258281.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2607bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 20-APR-2014
Organism Homo sapiens (human)
Product DNA mismatch repair protein Msh2 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC342099.1, AK304496.1 and CB250419.1. Transcript Variant: This variant (2) lacks an alternate segment of the first exon, including the translation start site, compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304496.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGGGGCCGG CAGGAGCAAA GAATCTGCAG AGTGTTGTGC TTAGTAAAAT GAATTTTGAA 
TCTTTTGTAA AAGATCTTCT TCTGGTTCGT CAGTATAGAG TTGAAGTTTA TAAGAATAGA
GCTGGAAATA AGGCATCCAA GGAGAATGAT TGGTATTTGG CATATAAGGC TTCTCCTGGC
AATCTCTCTC AGTTTGAAGA CATTCTCTTT GGTAACAATG ATATGTCAGC TTCCATTGGT
GTTGTGGGTG TTAAAATGTC CGCAGTTGAT GGCCAGAGAC AGGTTGGAGT TGGGTATGTG
GATTCCATAC AGAGGAAACT AGGACTGTGT GAATTCCCTG ATAATGATCA GTTCTCCAAT
CTTGAGGCTC TCCTCATCCA GATTGGACCA AAGGAATGTG TTTTACCCGG AGGAGAGACT
GCTGGAGACA TGGGGAAACT GAGACAGATA ATTCAAAGAG GAGGAATTCT GATCACAGAA
AGAAAAAAAG CTGACTTTTC CACAAAAGAC ATTTATCAGG ACCTCAACCG GTTGTTGAAA
GGCAAAAAGG GAGAGCAGAT GAATAGTGCT GTATTGCCAG AAATGGAGAA TCAGGTTGCA
GTTTCATCAC TGTCTGCGGT AATCAAGTTT TTAGAACTCT TATCAGATGA TTCCAACTTT
GGACAGTTTG AACTGACTAC TTTTGACTTC AGCCAGTATA TGAAATTGGA TATTGCAGCA
GTCAGAGCCC TTAACCTTTT TCAGGGTTCT GTTGAAGATA CCACTGGCTC TCAGTCTCTG
GCTGCCTTGC TGAATAAGTG TAAAACCCCT CAAGGACAAA GACTTGTTAA CCAGTGGATT
AAGCAGCCTC TCATGGATAA GAACAGAATA GAGGAGAGAT TGAATTTAGT GGAAGCTTTT
GTAGAAGATG CAGAATTGAG GCAGACTTTA CAAGAAGATT TACTTCGTCG ATTCCCAGAT
CTTAACCGAC TTGCCAAGAA GTTTCAAAGA CAAGCAGCAA ACTTACAAGA TTGTTACCGA
CTCTATCAGG GTATAAATCA ACTACCTAAT GTTATACAGG CTCTGGAAAA ACATGAAGGA
AAACACCAGA AATTATTGTT GGCAGTTTTT GTGACTCCTC TTACTGATCT TCGTTCTGAC
TTCTCCAAGT TTCAGGAAAT GATAGAAACA ACTTTAGATA TGGATCAGGT GGAAAACCAT
GAATTCCTTG TAAAACCTTC ATTTGATCCT AATCTCAGTG AATTAAGAGA AATAATGAAT
GACTTGGAAA AGAAGATGCA GTCAACATTA ATAAGTGCAG CCAGAGATCT TGGCTTGGAC
CCTGGCAAAC AGATTAAACT GGATTCCAGT GCACAGTTTG GATATTACTT TCGTGTAACC
TGTAAGGAAG AAAAAGTCCT TCGTAACAAT AAAAACTTTA GTACTGTAGA TATCCAGAAG
AATGGTGTTA AATTTACCAA CAGCAAATTG ACTTCTTTAA ATGAAGAGTA TACCAAAAAT
AAAACAGAAT ATGAAGAAGC CCAGGATGCC ATTGTTAAAG AAATTGTCAA TATTTCTTCA
GGCTATGTAG AACCAATGCA GACACTCAAT GATGTGTTAG CTCAGCTAGA TGCTGTTGTC
AGCTTTGCTC ACGTGTCAAA TGGAGCACCT GTTCCATATG TACGACCAGC CATTTTGGAG
AAAGGACAAG GAAGAATTAT ATTAAAAGCA TCCAGGCATG CTTGTGTTGA AGTTCAAGAT
GAAATTGCAT TTATTCCTAA TGACGTATAC TTTGAAAAAG ATAAACAGAT GTTCCACATC
ATTACTGGCC CCAATATGGG AGGTAAATCA ACATATATTC GACAAACTGG GGTGATAGTA
CTCATGGCCC AAATTGGGTG TTTTGTGCCA TGTGAGTCAG CAGAAGTGTC CATTGTGGAC
TGCATCTTAG CCCGAGTAGG GGCTGGTGAC AGTCAATTGA AAGGAGTCTC CACGTTCATG
GCTGAAATGT TGGAAACTGC TTCTATCCTC AGGTCTGCAA CCAAAGATTC ATTAATAATC
ATAGATGAAT TGGGAAGAGG AACTTCTACC TACGATGGAT TTGGGTTAGC ATGGGCTATA
TCAGAATACA TTGCAACAAA GATTGGTGCT TTTTGCATGT TTGCAACCCA TTTTCATGAA
CTTACTGCCT TGGCCAATCA GATACCAACT GTTAATAATC TACATGTCAC AGCACTCACC
ACTGAAGAGA CCTTAACTAT GCTTTATCAG GTGAAGAAAG GTGTCTGTGA TCAAAGTTTT
GGGATTCATG TTGCAGAGCT TGCTAATTTC CCTAAGCATG TAATAGAGTG TGCTAAACAG
AAAGCCCTGG AACTTGAGGA GTTTCAGTAT ATTGGAGAAT CGCAAGGATA TGATATCATG
GAACCAGCAG CAAAGAAGTG CTATCTGGAA AGAGAGCAAG GTGAAAAAAT TATTCAGGAG
TTCCTGTCCA AGGTGAAACA AATGCCCTTT ACTGAAATGT CAGAAGAAAA CATCACAATA
AAGTTAAAAC AGCTAAAAGC TGAAGTAATA GCAAAGAATA ATAGCTTTGT AAATGAAATC
ATTTCACGAA TAAAAGTTAC TACGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001245210.1
CDS140..2746
Misc Feature(1)5..7(+)
Misc Feature(2)152..337(+)
Misc Feature(3)413..793(+)
Misc Feature(4)854..1771(+)
Misc Feature(5)908..1876(+)
Misc Feature(6)1838..2497(+)
Misc Feature(7)1946..1969(+)
Misc Feature(8)1955..2290(+)
Misc Feature(9)2069..2080(+)
Misc Feature(10)2093..2134(+)
Misc Feature(11)2171..2188(+)
Misc Feature(12)2195..2206(+)
Misc Feature(13)2276..2296(+)
Exon (1)1..109
Gene:MSH2
Gene Synonym:
Exon (2)110..152
Gene:MSH2
Gene Synonym:
Exon (3)153..307
Gene:MSH2
Gene Synonym:
Exon (4)308..586
Gene:MSH2
Gene Synonym:
Exon (5)587..733
Gene:MSH2
Gene Synonym:
Exon (6)734..883
Gene:MSH2
Gene Synonym:
Exon (7)884..1017
Gene:MSH2
Gene Synonym:
Exon (8)1018..1217
Gene:MSH2
Gene Synonym:
Exon (9)1218..1327
Gene:MSH2
Gene Synonym:
Exon (10)1328..1451
Gene:MSH2
Gene Synonym:
Exon (11)1452..1602
Gene:MSH2
Gene Synonym:
Exon (12)1603..1700
Gene:MSH2
Gene Synonym:
Exon (13)1701..1946
Gene:MSH2
Gene Synonym:
Exon (14)1947..2151
Gene:MSH2
Gene Synonym:
Exon (15)2152..2399
Gene:MSH2
Gene Synonym:
Exon (16)2400..2575
Gene:MSH2
Gene Synonym:
Exon (17)2576..3025
Gene:MSH2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001258281
Organism Homo sapiens (human)
Definition Homo sapiens mutS homolog 2 (MSH2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001258281

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGGGGCCGG CAGGAGCAAA GAATCTGCAG AGTGTTGTGC TTAGTAAAAT GAATTTTGAA 
TCTTTTGTAA AAGATCTTCT TCTGGTTCGT CAGTATAGAG TTGAAGTTTA TAAGAATAGA
GCTGGAAATA AGGCATCCAA GGAGAATGAT TGGTATTTGG CATATAAGGC TTCTCCTGGC
AATCTCTCTC AGTTTGAAGA CATTCTCTTT GGTAACAATG ATATGTCAGC TTCCATTGGT
GTTGTGGGTG TTAAAATGTC CGCAGTTGAT GGCCAGAGAC AGGTTGGAGT TGGGTATGTG
GATTCCATAC AGAGGAAACT AGGACTGTGT GAATTCCCTG ATAATGATCA GTTCTCCAAT
CTTGAGGCTC TCCTCATCCA GATTGGACCA AAGGAATGTG TTTTACCCGG AGGAGAGACT
GCTGGAGACA TGGGGAAACT GAGACAGATA ATTCAAAGAG GAGGAATTCT GATCACAGAA
AGAAAAAAAG CTGACTTTTC CACAAAAGAC ATTTATCAGG ACCTCAACCG GTTGTTGAAA
GGCAAAAAGG GAGAGCAGAT GAATAGTGCT GTATTGCCAG AAATGGAGAA TCAGGTTGCA
GTTTCATCAC TGTCTGCGGT AATCAAGTTT TTAGAACTCT TATCAGATGA TTCCAACTTT
GGACAGTTTG AACTGACTAC TTTTGACTTC AGCCAGTATA TGAAATTGGA TATTGCAGCA
GTCAGAGCCC TTAACCTTTT TCAGGGTTCT GTTGAAGATA CCACTGGCTC TCAGTCTCTG
GCTGCCTTGC TGAATAAGTG TAAAACCCCT CAAGGACAAA GACTTGTTAA CCAGTGGATT
AAGCAGCCTC TCATGGATAA GAACAGAATA GAGGAGAGAT TGAATTTAGT GGAAGCTTTT
GTAGAAGATG CAGAATTGAG GCAGACTTTA CAAGAAGATT TACTTCGTCG ATTCCCAGAT
CTTAACCGAC TTGCCAAGAA GTTTCAAAGA CAAGCAGCAA ACTTACAAGA TTGTTACCGA
CTCTATCAGG GTATAAATCA ACTACCTAAT GTTATACAGG CTCTGGAAAA ACATGAAGGA
AAACACCAGA AATTATTGTT GGCAGTTTTT GTGACTCCTC TTACTGATCT TCGTTCTGAC
TTCTCCAAGT TTCAGGAAAT GATAGAAACA ACTTTAGATA TGGATCAGGT GGAAAACCAT
GAATTCCTTG TAAAACCTTC ATTTGATCCT AATCTCAGTG AATTAAGAGA AATAATGAAT
GACTTGGAAA AGAAGATGCA GTCAACATTA ATAAGTGCAG CCAGAGATCT TGGCTTGGAC
CCTGGCAAAC AGATTAAACT GGATTCCAGT GCACAGTTTG GATATTACTT TCGTGTAACC
TGTAAGGAAG AAAAAGTCCT TCGTAACAAT AAAAACTTTA GTACTGTAGA TATCCAGAAG
AATGGTGTTA AATTTACCAA CAGCAAATTG ACTTCTTTAA ATGAAGAGTA TACCAAAAAT
AAAACAGAAT ATGAAGAAGC CCAGGATGCC ATTGTTAAAG AAATTGTCAA TATTTCTTCA
GGCTATGTAG AACCAATGCA GACACTCAAT GATGTGTTAG CTCAGCTAGA TGCTGTTGTC
AGCTTTGCTC ACGTGTCAAA TGGAGCACCT GTTCCATATG TACGACCAGC CATTTTGGAG
AAAGGACAAG GAAGAATTAT ATTAAAAGCA TCCAGGCATG CTTGTGTTGA AGTTCAAGAT
GAAATTGCAT TTATTCCTAA TGACGTATAC TTTGAAAAAG ATAAACAGAT GTTCCACATC
ATTACTGGCC CCAATATGGG AGGTAAATCA ACATATATTC GACAAACTGG GGTGATAGTA
CTCATGGCCC AAATTGGGTG TTTTGTGCCA TGTGAGTCAG CAGAAGTGTC CATTGTGGAC
TGCATCTTAG CCCGAGTAGG GGCTGGTGAC AGTCAATTGA AAGGAGTCTC CACGTTCATG
GCTGAAATGT TGGAAACTGC TTCTATCCTC AGGTCTGCAA CCAAAGATTC ATTAATAATC
ATAGATGAAT TGGGAAGAGG AACTTCTACC TACGATGGAT TTGGGTTAGC ATGGGCTATA
TCAGAATACA TTGCAACAAA GATTGGTGCT TTTTGCATGT TTGCAACCCA TTTTCATGAA
CTTACTGCCT TGGCCAATCA GATACCAACT GTTAATAATC TACATGTCAC AGCACTCACC
ACTGAAGAGA CCTTAACTAT GCTTTATCAG GTGAAGAAAG GTGTCTGTGA TCAAAGTTTT
GGGATTCATG TTGCAGAGCT TGCTAATTTC CCTAAGCATG TAATAGAGTG TGCTAAACAG
AAAGCCCTGG AACTTGAGGA GTTTCAGTAT ATTGGAGAAT CGCAAGGATA TGATATCATG
GAACCAGCAG CAAAGAAGTG CTATCTGGAA AGAGAGCAAG GTGAAAAAAT TATTCAGGAG
TTCCTGTCCA AGGTGAAACA AATGCCCTTT ACTGAAATGT CAGAAGAAAA CATCACAATA
AAGTTAAAAC AGCTAAAAGC TGAAGTAATA GCAAAGAATA ATAGCTTTGT AAATGAAATC
ATTTCACGAA TAAAAGTTAC TACGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59053
Clone ID Related Accession (Same CDS sequence) XM_005264332
Accession Version XM_005264332.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2925bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA mismatch repair protein Msh2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022184.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530367622. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGGCGGTGC AGCCGAAGGA GACGCTGCAG TTGGAGAGCG CGGCCGAGGT CGGCTTCGTG 
CGCTTCTTTC AGGGCATGCC GGAGAAGCCG ACCACCACAG TGCGCCTTTT CGACCGGGGC
GACTTCTATA CGGCGCACGG CGAGGACGCG CTGCTGGCCG CCCGGGAGGT GTTCAAGACC
CAGGGGGTGA TCAAGTACAT GGGGCCGGCA GGAGCAAAGA ATCTGCAGAG TGTTGTGCTT
AGTAAAATGA ATTTTGAATC TTTTGTAAAA GATCTTCTTC TGGTTCGTCA GTATAGAGTT
GAAGTTTATA AGAATAGAGC TGGAAATAAG GCATCCAAGG AGAATGATTG GTATTTGGCA
TATAAGGCTT CTCCTGGCAA TCTCTCTCAG TTTGAAGACA TTCTCTTTGG TAACAATGAT
ATGTCAGCTT CCATTGGTGT TGTGGGTGTT AAAATGTCCG CAGTTGATGG CCAGAGACAG
GTTGGAGTTG GGTATGTGGA TTCCATACAG AGGAAACTAG GACTGTGTGA ATTCCCTGAT
AATGATCAGT TCTCCAATCT TGAGGCTCTC CTCATCCAGA TTGGACCAAA GGAATGTGTT
TTACCCGGAG GAGAGACTGC TGGAGACATG GGGAAACTGA GACAGATAAT TCAAAGAGGA
GGAATTCTGA TCACAGAAAG AAAAAAAGCT GACTTTTCCA CAAAAGACAT TTATCAGGAC
CTCAACCGGT TGTTGAAAGG CAAAAAGGGA GAGCAGATGA ATAGTGCTGT ATTGCCAGAA
ATGGAGAATC AGGTTGCAGT TTCATCACTG TCTGCGGTAA TCAAGTTTTT AGAACTCTTA
TCAGATGATT CCAACTTTGG ACAGTTTGAA CTGACTACTT TTGACTTCAG CCAGTATATG
AAATTGGATA TTGCAGCAGT CAGAGCCCTT AACCTTTTTC AGGGTTCTGT TGAAGATACC
ACTGGCTCTC AGTCTCTGGC TGCCTTGCTG AATAAGTGTA AAACCCCTCA AGGACAAAGA
CTTGTTAACC AGTGGATTAA GCAGCCTCTC ATGGATAAGA ACAGAATAGA GGAGAGATTG
AATTTAGTGG AAGCTTTTGT AGAAGATGCA GAATTGAGGC AGACTTTACA AGAAGATTTA
CTTCGTCGAT TCCCAGATCT TAACCGACTT GCCAAGAAGT TTCAAAGACA AGCAGCAAAC
TTACAAGATT GTTACCGACT CTATCAGGGT ATAAATCAAC TACCTAATGT TATACAGGCT
CTGGAAAAAC ATGAAGGAAA ACACCAGAAA TTATTGTTGG CAGTTTTTGT GACTCCTCTT
ACTGATCTTC GTTCTGACTT CTCCAAGTTT CAGGAAATGA TAGAAACAAC TTTAGATATG
GATCAGGTGG AAAACCATGA ATTCCTTGTA AAACCTTCAT TTGATCCTAA TCTCAGTGAA
TTAAGAGAAA TAATGAATGA CTTGGAAAAG AAGATGCAGT CAACATTAAT AAGTGCAGCC
AGAGATCTTG GCTTGGACCC TGGCAAACAG ATTAAACTGG ATTCCAGTGC ACAGTTTGGA
TATTACTTTC GTGTAACCTG TAAGGAAGAA AAAGTCCTTC GTAACAATAA AAACTTTAGT
ACTGTAGATA TCCAGAAGAA TGGTGTTAAA TTTACCAACA GCAAATTGAC TTCTTTAAAT
GAAGAGTATA CCAAAAATAA AACAGAATAT GAAGAAGCCC AGGATGCCAT TGTTAAAGAA
ATTGTCAATA TTTCTTCAGG CTATGTAGAA CCAATGCAGA CACTCAATGA TGTGTTAGCT
CAGCTAGATG CTGTTGTCAG CTTTGCTCAC GTGTCAAATG GAGCACCTGT TCCATATGTA
CGACCAGCCA TTTTGGAGAA AGGACAAGGA AGAATTATAT TAAAAGCATC CAGGCATGCT
TGTGTTGAAG TTCAAGATGA AATTGCATTT ATTCCTAATG ACGTATACTT TGAAAAAGAT
AAACAGATGT TCCACATCAT TACTGGCCCC AATATGGGAG GTAAATCAAC ATATATTCGA
CAAACTGGGG TGATAGTACT CATGGCCCAA ATTGGGTGTT TTGTGCCATG TGAGTCAGCA
GAAGTGTCCA TTGTGGACTG CATCTTAGCC CGAGTAGGGG CTGGTGACAG TCAATTGAAA
GGAGTCTCCA CGTTCATGGC TGAAATGTTG GAAACTGCTT CTATCCTCAG GTCTGCAACC
AAAGATTCAT TAATAATCAT AGATGAATTG GGAAGAGGAA CTTCTACCTA CGATGGATTT
GGGTTAGCAT GGGCTATATC AGAATACATT GCAACAAAGA TTGGTGCTTT TTGCATGTTT
GCAACCCATT TTCATGAACT TACTGCCTTG GCCAATCAGA TACCAACTGT TAATAATCTA
CATGTCACAG CACTCACCAC TGAAGAGACC TTAACTATGC TTTATCAGGT GAAGAAAGGT
GTCTGTGATC AAAGTTTTGG GATTCATGTT GCAGAGCTTG CTAATTTCCC TAAGCATGTA
ATAGAGTGTG CTAAACAGAA AGCCCTGGAA CTTGAGGAGT TTCAGTATAT TGGAGAATCG
CAAGGATATG ATATCATGGA ACCAGCAGCA AAGAAGTGCT ATCTGGAAAG AGAGAACCTC
AGAGTGACAG AGCCAAAAGA CCAGTGCCTC ATTTTGCTGA CATGGAAAAG GAAACTTCGT
GGGGGAAAGA GATCTGCTTG CAGTCGGCCA GAGAGACAGA ACCAGGGCAG TGGAGGAGAT
TTTAAAAGGA AGTCTCTGGT GGTTTGTAAA GCAACAAATC CTGCTCTCAA GTGGATAGTT
CCAAGCCCTC TCAATGAATT CAGTTTTATA CACCTGGAGA AGCACAGCCT CGTCCTTTCC
ATGGAGCTAC AAGCCACATC TGGGGGCGCT CAGTGCCCAG GCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005264389.2
CDS73..2997
Misc Feature(1)124..468(+)
Misc Feature(2)139..2631(+)
Misc Feature(3)544..924(+)
Misc Feature(4)985..1902(+)
Misc Feature(5)1969..2628(+)
Misc Feature(6)2077..2100(+)
Misc Feature(7)2086..2421(+)
Misc Feature(8)2200..2211(+)
Misc Feature(9)2224..2265(+)
Misc Feature(10)2302..2319(+)
Misc Feature(11)2326..2337(+)
Misc Feature(12)2407..2427(+)
Translation

Target ORF information:

RefSeq Version XM_005264332
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mutS homolog 2 (MSH2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005264332

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGGCGGTGC AGCCGAAGGA GACGCTGCAG TTGGAGAGCG CGGCCGAGGT CGGCTTCGTG 
CGCTTCTTTC AGGGCATGCC GGAGAAGCCG ACCACCACAG TGCGCCTTTT CGACCGGGGC
GACTTCTATA CGGCGCACGG CGAGGACGCG CTGCTGGCCG CCCGGGAGGT GTTCAAGACC
CAGGGGGTGA TCAAGTACAT GGGGCCGGCA GGAGCAAAGA ATCTGCAGAG TGTTGTGCTT
AGTAAAATGA ATTTTGAATC TTTTGTAAAA GATCTTCTTC TGGTTCGTCA GTATAGAGTT
GAAGTTTATA AGAATAGAGC TGGAAATAAG GCATCCAAGG AGAATGATTG GTATTTGGCA
TATAAGGCTT CTCCTGGCAA TCTCTCTCAG TTTGAAGACA TTCTCTTTGG TAACAATGAT
ATGTCAGCTT CCATTGGTGT TGTGGGTGTT AAAATGTCCG CAGTTGATGG CCAGAGACAG
GTTGGAGTTG GGTATGTGGA TTCCATACAG AGGAAACTAG GACTGTGTGA ATTCCCTGAT
AATGATCAGT TCTCCAATCT TGAGGCTCTC CTCATCCAGA TTGGACCAAA GGAATGTGTT
TTACCCGGAG GAGAGACTGC TGGAGACATG GGGAAACTGA GACAGATAAT TCAAAGAGGA
GGAATTCTGA TCACAGAAAG AAAAAAAGCT GACTTTTCCA CAAAAGACAT TTATCAGGAC
CTCAACCGGT TGTTGAAAGG CAAAAAGGGA GAGCAGATGA ATAGTGCTGT ATTGCCAGAA
ATGGAGAATC AGGTTGCAGT TTCATCACTG TCTGCGGTAA TCAAGTTTTT AGAACTCTTA
TCAGATGATT CCAACTTTGG ACAGTTTGAA CTGACTACTT TTGACTTCAG CCAGTATATG
AAATTGGATA TTGCAGCAGT CAGAGCCCTT AACCTTTTTC AGGGTTCTGT TGAAGATACC
ACTGGCTCTC AGTCTCTGGC TGCCTTGCTG AATAAGTGTA AAACCCCTCA AGGACAAAGA
CTTGTTAACC AGTGGATTAA GCAGCCTCTC ATGGATAAGA ACAGAATAGA GGAGAGATTG
AATTTAGTGG AAGCTTTTGT AGAAGATGCA GAATTGAGGC AGACTTTACA AGAAGATTTA
CTTCGTCGAT TCCCAGATCT TAACCGACTT GCCAAGAAGT TTCAAAGACA AGCAGCAAAC
TTACAAGATT GTTACCGACT CTATCAGGGT ATAAATCAAC TACCTAATGT TATACAGGCT
CTGGAAAAAC ATGAAGGAAA ACACCAGAAA TTATTGTTGG CAGTTTTTGT GACTCCTCTT
ACTGATCTTC GTTCTGACTT CTCCAAGTTT CAGGAAATGA TAGAAACAAC TTTAGATATG
GATCAGGTGG AAAACCATGA ATTCCTTGTA AAACCTTCAT TTGATCCTAA TCTCAGTGAA
TTAAGAGAAA TAATGAATGA CTTGGAAAAG AAGATGCAGT CAACATTAAT AAGTGCAGCC
AGAGATCTTG GCTTGGACCC TGGCAAACAG ATTAAACTGG ATTCCAGTGC ACAGTTTGGA
TATTACTTTC GTGTAACCTG TAAGGAAGAA AAAGTCCTTC GTAACAATAA AAACTTTAGT
ACTGTAGATA TCCAGAAGAA TGGTGTTAAA TTTACCAACA GCAAATTGAC TTCTTTAAAT
GAAGAGTATA CCAAAAATAA AACAGAATAT GAAGAAGCCC AGGATGCCAT TGTTAAAGAA
ATTGTCAATA TTTCTTCAGG CTATGTAGAA CCAATGCAGA CACTCAATGA TGTGTTAGCT
CAGCTAGATG CTGTTGTCAG CTTTGCTCAC GTGTCAAATG GAGCACCTGT TCCATATGTA
CGACCAGCCA TTTTGGAGAA AGGACAAGGA AGAATTATAT TAAAAGCATC CAGGCATGCT
TGTGTTGAAG TTCAAGATGA AATTGCATTT ATTCCTAATG ACGTATACTT TGAAAAAGAT
AAACAGATGT TCCACATCAT TACTGGCCCC AATATGGGAG GTAAATCAAC ATATATTCGA
CAAACTGGGG TGATAGTACT CATGGCCCAA ATTGGGTGTT TTGTGCCATG TGAGTCAGCA
GAAGTGTCCA TTGTGGACTG CATCTTAGCC CGAGTAGGGG CTGGTGACAG TCAATTGAAA
GGAGTCTCCA CGTTCATGGC TGAAATGTTG GAAACTGCTT CTATCCTCAG GTCTGCAACC
AAAGATTCAT TAATAATCAT AGATGAATTG GGAAGAGGAA CTTCTACCTA CGATGGATTT
GGGTTAGCAT GGGCTATATC AGAATACATT GCAACAAAGA TTGGTGCTTT TTGCATGTTT
GCAACCCATT TTCATGAACT TACTGCCTTG GCCAATCAGA TACCAACTGT TAATAATCTA
CATGTCACAG CACTCACCAC TGAAGAGACC TTAACTATGC TTTATCAGGT GAAGAAAGGT
GTCTGTGATC AAAGTTTTGG GATTCATGTT GCAGAGCTTG CTAATTTCCC TAAGCATGTA
ATAGAGTGTG CTAAACAGAA AGCCCTGGAA CTTGAGGAGT TTCAGTATAT TGGAGAATCG
CAAGGATATG ATATCATGGA ACCAGCAGCA AAGAAGTGCT ATCTGGAAAG AGAGAACCTC
AGAGTGACAG AGCCAAAAGA CCAGTGCCTC ATTTTGCTGA CATGGAAAAG GAAACTTCGT
GGGGGAAAGA GATCTGCTTG CAGTCGGCCA GAGAGACAGA ACCAGGGCAG TGGAGGAGAT
TTTAAAAGGA AGTCTCTGGT GGTTTGTAAA GCAACAAATC CTGCTCTCAA GTGGATAGTT
CCAAGCCCTC TCAATGAATT CAGTTTTATA CACCTGGAGA AGCACAGCCT CGTCCTTTCC
ATGGAGCTAC AAGCCACATC TGGGGGCGCT CAGTGCCCAG GCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59054
Clone ID Related Accession (Same CDS sequence) XM_011532867
Accession Version XM_011532867.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA mismatch repair protein Msh2 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022184.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGGCGGTGC AGCCGAAGGA GACGCTGCAG TTGGAGAGCG CGGCCGAGGT CGGCTTCGTG 
CGCTTCTTTC AGGGCATGCC GGAGAAGCCG ACCACCACAG TGCGCCTTTT CGACCGGGGC
GACTTCTATA CGGCGCACGG CGAGGACGCG CTGCTGGCCG CCCGGGAGGT GTTCAAGACC
CAGGGGGTGA TCAAGTACAT GGGGCCGGCA GGAGCAAAGA ATCTGCAGAG TGTTGTGCTT
AGTAAAATGA ATTTTGAATC TTTTGTAAAA GATCTTCTTC TGGTTCGTCA GTATAGAGTT
GAAGTTTATA AGAATAGAGC TGGAAATAAG GCATCCAAGG AGAATGATTG GTATTTGGCA
TATAAGGCTT CTCCTGGCAA TCTCTCTCAG TTTGAAGACA TTCTCTTTGG TAACAATGAT
ATGTCAGCTT CCATTGGTGT TGTGGGTGTT AAAATGTCCG CAGTTGATGG CCAGAGACAG
GTTGGAGTTG GGTATGTGGA TTCCATACAG AGGAAACTAG GACTGTGTGA ATTCCCTGAT
AATGATCAGT TCTCCAATCT TGAGGCTCTC CTCATCCAGA TTGGACCAAA GGAATGTGTT
TTACCCGGAG GAGAGACTGC TGGAGACATG GGGAAACTGA GACAGATAAT TCAAAGAGGA
GGAATTCTGA TCACAGAAAG AAAAAAAGCT GACTTTTCCA CAAAAGACAT TTATCAGGAC
CTCAACCGGT TGTTGAAAGG CAAAAAGGGA GAGCAGATGA ATAGTGCTGT ATTGCCAGAA
ATGGAGAATC AGGTTGCAGT TTCATCACTG TCTGCGGTAA TCAAGTTTTT AGAACTCTTA
TCAGATGATT CCAACTTTGG ACAGTTTGAA CTGACTACTT TTGACTTCAG CCAGTATATG
AAATTGGATA TTGCAGCAGT CAGAGCCCTT AACCTTTTTC AGGGTTCTGT TGAAGATACC
ACTGGCTCTC AGTCTCTGGC TGCCTTGCTG AATAAGTGTA AAACCCCTCA AGGACAAAGA
CTTGTTAACC AGTGGATTAA GCAGCCTCTC ATGGATAAGA ACAGAATAGA GGAGAGATTG
AATTTAGTGG AAGCTTTTGT AGAAGATGCA GAATTGAGGC AGACTTTACA AGAAGATTTA
CTTCGTCGAT TCCCAGATCT TAACCGACTT GCCAAGAAGT TTCAAAGACA AGCAGCAAAC
TTACAAGATT GTTACCGACT CTATCAGGGT ATAAATCAAC TACCTAATGT TATACAGGCT
CTGGAAAAAC ATGAAGGAAA ACACCAGAAA TTATTGTTGG CAGTTTTTGT GACTCCTCTT
ACTGATCTTC GTTCTGACTT CTCCAAGTTT CAGGAAATGA TAGAAACAAC TTTAGATATG
GATCAGGTGG AAAACCATGA ATTCCTTGTA AAACCTTCAT TTGATCCTAA TCTCAGTGAA
TTAAGAGAAA TAATGAATGA CTTGGAAAAG AAGATGCAGT CAACATTAAT AAGTGCAGCC
AGAGATCTTG GCTTGGACCC TGGCAAACAG ATTAAACTGG ATTCCAGTGC ACAGTTTGGA
TATTACTTTC GTGTAACCTG TAAGGAAGAA AAAGTCCTTC GTAACAATAA AAACTTTAGT
ACTGTAGATA TCCAGAAGAA TGGTGTTAAA TTTACCAACA GCAAATTGAC TTCTTTAAAT
GAAGAGTATA CCAAAAATAA AACAGAATAT GAAGAAGCCC AGGATGCCAT TGTTAAAGAA
ATTGTCAATA TTTCTTCAGG CTATGTAGAA CCAATGCAGA CACTCAATGA TGTGTTAGCT
CAGCTAGATG CTGTTGTCAG CTTTGCTCAC GTGTCAAATG GAGCACCTGT TCCATATGTA
CGACCAGCCA TTTTGGAGAA AGGACAAGGA AGAATTATAT TAAAAGCATC CAGGCATGCT
TGTGTTGAAG TTCAAGATGA AATTGCATTT ATTCCTAATG ACGTATACTT TGAAAAAGAT
AAACAGATGT TCCACATCAT TACTGGCCCC AATATGGGAG GTAAATCAAC ATATATTCGA
CAAACTGGGG TGATAGTACT CATGGCCCAA ATTGGGTGTT TTGTGCCATG TGAGTCAGCA
GAAGTGTCCA TTGTGGACTG CATCTTAGCC CGAGTAGGGG CTGGTGACAG TCAATTGAAA
GGAGTCTCCA CGTTCATGGC TGAAATGTTG GAAACTGCTT CTATCCTCAG GTCTGCAACC
AAAGATTCAT TAATAATCAT AGATGAATTG GGAAGAGGAA CTTCTACCTA CGATGGATTT
GGGTTAGCAT GGGCTATATC AGAATACATT GCAACAAAGA TTGGTGCTTT TTGCATGTTT
GCAACCCATT TTCATGAACT TACTGCCTTG GCCAATCAGA TACCAACTGT TAATAATCTA
CATGTCACAG CACTCACCAC TGAAGAGACC TTAACTATGC TTTATCAGGT GAAGAAAGGT
GTCTGTGATC AAAGTTTTGG GATTCATGTT GCAGAGCTTG CTAATTTCCC TAAGCATGTA
ATAGAGTGTG CTAAACAGAA AGCCCTGGAA CTTGAGGAGT TTCAGTATAT TGGAGAATCG
CAAGGATATG ATATCATGGA ACCAGCAGCA AAGAAGTGCT ATCTGGAAAG AGAGAACCTC
AGAGTGACAG AGCCAAAAGA CCAGTGCCTC ATTTTGCTGA CATGGAAAAG GAAACTTCGT
GGGGGAAAGA GATCTGCTTG CAGTCGGCCA GAGAGACAGA ACCAGGGCAG TGCGACTCCA
AGTGCATCAG CATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011531169.1
CDS73..2847
Misc Feature(1)124..468(+)
Misc Feature(2)139..2631(+)
Misc Feature(3)544..924(+)
Misc Feature(4)985..1902(+)
Misc Feature(5)1969..2628(+)
Misc Feature(6)2077..2100(+)
Misc Feature(7)2086..2421(+)
Misc Feature(8)2200..2211(+)
Misc Feature(9)2224..2265(+)
Misc Feature(10)2302..2319(+)
Misc Feature(11)2326..2337(+)
Misc Feature(12)2407..2427(+)
Translation

Target ORF information:

RefSeq Version XM_011532867
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mutS homolog 2 (MSH2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011532867

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGGCGGTGC AGCCGAAGGA GACGCTGCAG TTGGAGAGCG CGGCCGAGGT CGGCTTCGTG 
CGCTTCTTTC AGGGCATGCC GGAGAAGCCG ACCACCACAG TGCGCCTTTT CGACCGGGGC
GACTTCTATA CGGCGCACGG CGAGGACGCG CTGCTGGCCG CCCGGGAGGT GTTCAAGACC
CAGGGGGTGA TCAAGTACAT GGGGCCGGCA GGAGCAAAGA ATCTGCAGAG TGTTGTGCTT
AGTAAAATGA ATTTTGAATC TTTTGTAAAA GATCTTCTTC TGGTTCGTCA GTATAGAGTT
GAAGTTTATA AGAATAGAGC TGGAAATAAG GCATCCAAGG AGAATGATTG GTATTTGGCA
TATAAGGCTT CTCCTGGCAA TCTCTCTCAG TTTGAAGACA TTCTCTTTGG TAACAATGAT
ATGTCAGCTT CCATTGGTGT TGTGGGTGTT AAAATGTCCG CAGTTGATGG CCAGAGACAG
GTTGGAGTTG GGTATGTGGA TTCCATACAG AGGAAACTAG GACTGTGTGA ATTCCCTGAT
AATGATCAGT TCTCCAATCT TGAGGCTCTC CTCATCCAGA TTGGACCAAA GGAATGTGTT
TTACCCGGAG GAGAGACTGC TGGAGACATG GGGAAACTGA GACAGATAAT TCAAAGAGGA
GGAATTCTGA TCACAGAAAG AAAAAAAGCT GACTTTTCCA CAAAAGACAT TTATCAGGAC
CTCAACCGGT TGTTGAAAGG CAAAAAGGGA GAGCAGATGA ATAGTGCTGT ATTGCCAGAA
ATGGAGAATC AGGTTGCAGT TTCATCACTG TCTGCGGTAA TCAAGTTTTT AGAACTCTTA
TCAGATGATT CCAACTTTGG ACAGTTTGAA CTGACTACTT TTGACTTCAG CCAGTATATG
AAATTGGATA TTGCAGCAGT CAGAGCCCTT AACCTTTTTC AGGGTTCTGT TGAAGATACC
ACTGGCTCTC AGTCTCTGGC TGCCTTGCTG AATAAGTGTA AAACCCCTCA AGGACAAAGA
CTTGTTAACC AGTGGATTAA GCAGCCTCTC ATGGATAAGA ACAGAATAGA GGAGAGATTG
AATTTAGTGG AAGCTTTTGT AGAAGATGCA GAATTGAGGC AGACTTTACA AGAAGATTTA
CTTCGTCGAT TCCCAGATCT TAACCGACTT GCCAAGAAGT TTCAAAGACA AGCAGCAAAC
TTACAAGATT GTTACCGACT CTATCAGGGT ATAAATCAAC TACCTAATGT TATACAGGCT
CTGGAAAAAC ATGAAGGAAA ACACCAGAAA TTATTGTTGG CAGTTTTTGT GACTCCTCTT
ACTGATCTTC GTTCTGACTT CTCCAAGTTT CAGGAAATGA TAGAAACAAC TTTAGATATG
GATCAGGTGG AAAACCATGA ATTCCTTGTA AAACCTTCAT TTGATCCTAA TCTCAGTGAA
TTAAGAGAAA TAATGAATGA CTTGGAAAAG AAGATGCAGT CAACATTAAT AAGTGCAGCC
AGAGATCTTG GCTTGGACCC TGGCAAACAG ATTAAACTGG ATTCCAGTGC ACAGTTTGGA
TATTACTTTC GTGTAACCTG TAAGGAAGAA AAAGTCCTTC GTAACAATAA AAACTTTAGT
ACTGTAGATA TCCAGAAGAA TGGTGTTAAA TTTACCAACA GCAAATTGAC TTCTTTAAAT
GAAGAGTATA CCAAAAATAA AACAGAATAT GAAGAAGCCC AGGATGCCAT TGTTAAAGAA
ATTGTCAATA TTTCTTCAGG CTATGTAGAA CCAATGCAGA CACTCAATGA TGTGTTAGCT
CAGCTAGATG CTGTTGTCAG CTTTGCTCAC GTGTCAAATG GAGCACCTGT TCCATATGTA
CGACCAGCCA TTTTGGAGAA AGGACAAGGA AGAATTATAT TAAAAGCATC CAGGCATGCT
TGTGTTGAAG TTCAAGATGA AATTGCATTT ATTCCTAATG ACGTATACTT TGAAAAAGAT
AAACAGATGT TCCACATCAT TACTGGCCCC AATATGGGAG GTAAATCAAC ATATATTCGA
CAAACTGGGG TGATAGTACT CATGGCCCAA ATTGGGTGTT TTGTGCCATG TGAGTCAGCA
GAAGTGTCCA TTGTGGACTG CATCTTAGCC CGAGTAGGGG CTGGTGACAG TCAATTGAAA
GGAGTCTCCA CGTTCATGGC TGAAATGTTG GAAACTGCTT CTATCCTCAG GTCTGCAACC
AAAGATTCAT TAATAATCAT AGATGAATTG GGAAGAGGAA CTTCTACCTA CGATGGATTT
GGGTTAGCAT GGGCTATATC AGAATACATT GCAACAAAGA TTGGTGCTTT TTGCATGTTT
GCAACCCATT TTCATGAACT TACTGCCTTG GCCAATCAGA TACCAACTGT TAATAATCTA
CATGTCACAG CACTCACCAC TGAAGAGACC TTAACTATGC TTTATCAGGT GAAGAAAGGT
GTCTGTGATC AAAGTTTTGG GATTCATGTT GCAGAGCTTG CTAATTTCCC TAAGCATGTA
ATAGAGTGTG CTAAACAGAA AGCCCTGGAA CTTGAGGAGT TTCAGTATAT TGGAGAATCG
CAAGGATATG ATATCATGGA ACCAGCAGCA AAGAAGTGCT ATCTGGAAAG AGAGAACCTC
AGAGTGACAG AGCCAAAAGA CCAGTGCCTC ATTTTGCTGA CATGGAAAAG GAAACTTCGT
GGGGGAAAGA GATCTGCTTG CAGTCGGCCA GAGAGACAGA ACCAGGGCAG TGCGACTCCA
AGTGCATCAG CATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20167
Clone ID Related Accession (Same CDS sequence) NM_000251
Accession Version NM_000251.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2805bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product DNA mismatch repair protein Msh2 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC342099.1, U04045.1, AK304496.1 and CB250419.1. This sequence is a reference standard in the RefSeqGene project. On Apr 20, 2012 this sequence version replaced gi:4557760. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX649122.1, BC021566.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGGCGGTGC AGCCGAAGGA GACGCTGCAG TTGGAGAGCG CGGCCGAGGT CGGCTTCGTG 
CGCTTCTTTC AGGGCATGCC GGAGAAGCCG ACCACCACAG TGCGCCTTTT CGACCGGGGC
GACTTCTATA CGGCGCACGG CGAGGACGCG CTGCTGGCCG CCCGGGAGGT GTTCAAGACC
CAGGGGGTGA TCAAGTACAT GGGGCCGGCA GGAGCAAAGA ATCTGCAGAG TGTTGTGCTT
AGTAAAATGA ATTTTGAATC TTTTGTAAAA GATCTTCTTC TGGTTCGTCA GTATAGAGTT
GAAGTTTATA AGAATAGAGC TGGAAATAAG GCATCCAAGG AGAATGATTG GTATTTGGCA
TATAAGGCTT CTCCTGGCAA TCTCTCTCAG TTTGAAGACA TTCTCTTTGG TAACAATGAT
ATGTCAGCTT CCATTGGTGT TGTGGGTGTT AAAATGTCCG CAGTTGATGG CCAGAGACAG
GTTGGAGTTG GGTATGTGGA TTCCATACAG AGGAAACTAG GACTGTGTGA ATTCCCTGAT
AATGATCAGT TCTCCAATCT TGAGGCTCTC CTCATCCAGA TTGGACCAAA GGAATGTGTT
TTACCCGGAG GAGAGACTGC TGGAGACATG GGGAAACTGA GACAGATAAT TCAAAGAGGA
GGAATTCTGA TCACAGAAAG AAAAAAAGCT GACTTTTCCA CAAAAGACAT TTATCAGGAC
CTCAACCGGT TGTTGAAAGG CAAAAAGGGA GAGCAGATGA ATAGTGCTGT ATTGCCAGAA
ATGGAGAATC AGGTTGCAGT TTCATCACTG TCTGCGGTAA TCAAGTTTTT AGAACTCTTA
TCAGATGATT CCAACTTTGG ACAGTTTGAA CTGACTACTT TTGACTTCAG CCAGTATATG
AAATTGGATA TTGCAGCAGT CAGAGCCCTT AACCTTTTTC AGGGTTCTGT TGAAGATACC
ACTGGCTCTC AGTCTCTGGC TGCCTTGCTG AATAAGTGTA AAACCCCTCA AGGACAAAGA
CTTGTTAACC AGTGGATTAA GCAGCCTCTC ATGGATAAGA ACAGAATAGA GGAGAGATTG
AATTTAGTGG AAGCTTTTGT AGAAGATGCA GAATTGAGGC AGACTTTACA AGAAGATTTA
CTTCGTCGAT TCCCAGATCT TAACCGACTT GCCAAGAAGT TTCAAAGACA AGCAGCAAAC
TTACAAGATT GTTACCGACT CTATCAGGGT ATAAATCAAC TACCTAATGT TATACAGGCT
CTGGAAAAAC ATGAAGGAAA ACACCAGAAA TTATTGTTGG CAGTTTTTGT GACTCCTCTT
ACTGATCTTC GTTCTGACTT CTCCAAGTTT CAGGAAATGA TAGAAACAAC TTTAGATATG
GATCAGGTGG AAAACCATGA ATTCCTTGTA AAACCTTCAT TTGATCCTAA TCTCAGTGAA
TTAAGAGAAA TAATGAATGA CTTGGAAAAG AAGATGCAGT CAACATTAAT AAGTGCAGCC
AGAGATCTTG GCTTGGACCC TGGCAAACAG ATTAAACTGG ATTCCAGTGC ACAGTTTGGA
TATTACTTTC GTGTAACCTG TAAGGAAGAA AAAGTCCTTC GTAACAATAA AAACTTTAGT
ACTGTAGATA TCCAGAAGAA TGGTGTTAAA TTTACCAACA GCAAATTGAC TTCTTTAAAT
GAAGAGTATA CCAAAAATAA AACAGAATAT GAAGAAGCCC AGGATGCCAT TGTTAAAGAA
ATTGTCAATA TTTCTTCAGG CTATGTAGAA CCAATGCAGA CACTCAATGA TGTGTTAGCT
CAGCTAGATG CTGTTGTCAG CTTTGCTCAC GTGTCAAATG GAGCACCTGT TCCATATGTA
CGACCAGCCA TTTTGGAGAA AGGACAAGGA AGAATTATAT TAAAAGCATC CAGGCATGCT
TGTGTTGAAG TTCAAGATGA AATTGCATTT ATTCCTAATG ACGTATACTT TGAAAAAGAT
AAACAGATGT TCCACATCAT TACTGGCCCC AATATGGGAG GTAAATCAAC ATATATTCGA
CAAACTGGGG TGATAGTACT CATGGCCCAA ATTGGGTGTT TTGTGCCATG TGAGTCAGCA
GAAGTGTCCA TTGTGGACTG CATCTTAGCC CGAGTAGGGG CTGGTGACAG TCAATTGAAA
GGAGTCTCCA CGTTCATGGC TGAAATGTTG GAAACTGCTT CTATCCTCAG GTCTGCAACC
AAAGATTCAT TAATAATCAT AGATGAATTG GGAAGAGGAA CTTCTACCTA CGATGGATTT
GGGTTAGCAT GGGCTATATC AGAATACATT GCAACAAAGA TTGGTGCTTT TTGCATGTTT
GCAACCCATT TTCATGAACT TACTGCCTTG GCCAATCAGA TACCAACTGT TAATAATCTA
CATGTCACAG CACTCACCAC TGAAGAGACC TTAACTATGC TTTATCAGGT GAAGAAAGGT
GTCTGTGATC AAAGTTTTGG GATTCATGTT GCAGAGCTTG CTAATTTCCC TAAGCATGTA
ATAGAGTGTG CTAAACAGAA AGCCCTGGAA CTTGAGGAGT TTCAGTATAT TGGAGAATCG
CAAGGATATG ATATCATGGA ACCAGCAGCA AAGAAGTGCT ATCTGGAAAG AGAGCAAGGT
GAAAAAATTA TTCAGGAGTT CCTGTCCAAG GTGAAACAAA TGCCCTTTAC TGAAATGTCA
GAAGAAAACA TCACAATAAA GTTAAAACAG CTAAAAGCTG AAGTAATAGC AAAGAATAAT
AGCTTTGTAA ATGAAATCAT TTCACGAATA AAAGTTACTA CGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000242.1
CDS126..2930
Misc Feature(1)111..113(+)
Misc Feature(2)129..131(+)
Misc Feature(3)177..521(+)
Misc Feature(4)192..2684(+)
Misc Feature(5)597..977(+)
Misc Feature(6)1038..1955(+)
Misc Feature(7)1788..1790(+)
Misc Feature(8)1926..2138(+)
Misc Feature(9)2022..2681(+)
Misc Feature(10)2130..2153(+)
Misc Feature(11)2139..2474(+)
Misc Feature(12)2253..2264(+)
Misc Feature(13)2277..2318(+)
Misc Feature(14)2355..2372(+)
Misc Feature(15)2379..2390(+)
Misc Feature(16)2460..2480(+)
Misc Feature(17)2703..2705(+)
Exon (1)1..336
Gene:MSH2
Gene Synonym:
Exon (2)337..491
Gene:MSH2
Gene Synonym:
Exon (3)492..770
Gene:MSH2
Gene Synonym:
Exon (4)771..917
Gene:MSH2
Gene Synonym:
Exon (5)918..1067
Gene:MSH2
Gene Synonym:
Exon (6)1068..1201
Gene:MSH2
Gene Synonym:
Exon (7)1202..1401
Gene:MSH2
Gene Synonym:
Exon (8)1402..1511
Gene:MSH2
Gene Synonym:
Exon (9)1512..1635
Gene:MSH2
Gene Synonym:
Exon (10)1636..1786
Gene:MSH2
Gene Synonym:
Exon (11)1787..1884
Gene:MSH2
Gene Synonym:
Exon (12)1885..2130
Gene:MSH2
Gene Synonym:
Exon (13)2131..2335
Gene:MSH2
Gene Synonym:
Exon (14)2336..2583
Gene:MSH2
Gene Synonym:
Exon (15)2584..2759
Gene:MSH2
Gene Synonym:
Exon (16)2760..3209
Gene:MSH2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000251
Organism Homo sapiens (human)
Definition Homo sapiens mutS homolog 2 (MSH2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000251

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGGCGGTGC AGCCGAAGGA GACGCTGCAG TTGGAGAGCG CGGCCGAGGT CGGCTTCGTG 
CGCTTCTTTC AGGGCATGCC GGAGAAGCCG ACCACCACAG TGCGCCTTTT CGACCGGGGC
GACTTCTATA CGGCGCACGG CGAGGACGCG CTGCTGGCCG CCCGGGAGGT GTTCAAGACC
CAGGGGGTGA TCAAGTACAT GGGGCCGGCA GGAGCAAAGA ATCTGCAGAG TGTTGTGCTT
AGTAAAATGA ATTTTGAATC TTTTGTAAAA GATCTTCTTC TGGTTCGTCA GTATAGAGTT
GAAGTTTATA AGAATAGAGC TGGAAATAAG GCATCCAAGG AGAATGATTG GTATTTGGCA
TATAAGGCTT CTCCTGGCAA TCTCTCTCAG TTTGAAGACA TTCTCTTTGG TAACAATGAT
ATGTCAGCTT CCATTGGTGT TGTGGGTGTT AAAATGTCCG CAGTTGATGG CCAGAGACAG
GTTGGAGTTG GGTATGTGGA TTCCATACAG AGGAAACTAG GACTGTGTGA ATTCCCTGAT
AATGATCAGT TCTCCAATCT TGAGGCTCTC CTCATCCAGA TTGGACCAAA GGAATGTGTT
TTACCCGGAG GAGAGACTGC TGGAGACATG GGGAAACTGA GACAGATAAT TCAAAGAGGA
GGAATTCTGA TCACAGAAAG AAAAAAAGCT GACTTTTCCA CAAAAGACAT TTATCAGGAC
CTCAACCGGT TGTTGAAAGG CAAAAAGGGA GAGCAGATGA ATAGTGCTGT ATTGCCAGAA
ATGGAGAATC AGGTTGCAGT TTCATCACTG TCTGCGGTAA TCAAGTTTTT AGAACTCTTA
TCAGATGATT CCAACTTTGG ACAGTTTGAA CTGACTACTT TTGACTTCAG CCAGTATATG
AAATTGGATA TTGCAGCAGT CAGAGCCCTT AACCTTTTTC AGGGTTCTGT TGAAGATACC
ACTGGCTCTC AGTCTCTGGC TGCCTTGCTG AATAAGTGTA AAACCCCTCA AGGACAAAGA
CTTGTTAACC AGTGGATTAA GCAGCCTCTC ATGGATAAGA ACAGAATAGA GGAGAGATTG
AATTTAGTGG AAGCTTTTGT AGAAGATGCA GAATTGAGGC AGACTTTACA AGAAGATTTA
CTTCGTCGAT TCCCAGATCT TAACCGACTT GCCAAGAAGT TTCAAAGACA AGCAGCAAAC
TTACAAGATT GTTACCGACT CTATCAGGGT ATAAATCAAC TACCTAATGT TATACAGGCT
CTGGAAAAAC ATGAAGGAAA ACACCAGAAA TTATTGTTGG CAGTTTTTGT GACTCCTCTT
ACTGATCTTC GTTCTGACTT CTCCAAGTTT CAGGAAATGA TAGAAACAAC TTTAGATATG
GATCAGGTGG AAAACCATGA ATTCCTTGTA AAACCTTCAT TTGATCCTAA TCTCAGTGAA
TTAAGAGAAA TAATGAATGA CTTGGAAAAG AAGATGCAGT CAACATTAAT AAGTGCAGCC
AGAGATCTTG GCTTGGACCC TGGCAAACAG ATTAAACTGG ATTCCAGTGC ACAGTTTGGA
TATTACTTTC GTGTAACCTG TAAGGAAGAA AAAGTCCTTC GTAACAATAA AAACTTTAGT
ACTGTAGATA TCCAGAAGAA TGGTGTTAAA TTTACCAACA GCAAATTGAC TTCTTTAAAT
GAAGAGTATA CCAAAAATAA AACAGAATAT GAAGAAGCCC AGGATGCCAT TGTTAAAGAA
ATTGTCAATA TTTCTTCAGG CTATGTAGAA CCAATGCAGA CACTCAATGA TGTGTTAGCT
CAGCTAGATG CTGTTGTCAG CTTTGCTCAC GTGTCAAATG GAGCACCTGT TCCATATGTA
CGACCAGCCA TTTTGGAGAA AGGACAAGGA AGAATTATAT TAAAAGCATC CAGGCATGCT
TGTGTTGAAG TTCAAGATGA AATTGCATTT ATTCCTAATG ACGTATACTT TGAAAAAGAT
AAACAGATGT TCCACATCAT TACTGGCCCC AATATGGGAG GTAAATCAAC ATATATTCGA
CAAACTGGGG TGATAGTACT CATGGCCCAA ATTGGGTGTT TTGTGCCATG TGAGTCAGCA
GAAGTGTCCA TTGTGGACTG CATCTTAGCC CGAGTAGGGG CTGGTGACAG TCAATTGAAA
GGAGTCTCCA CGTTCATGGC TGAAATGTTG GAAACTGCTT CTATCCTCAG GTCTGCAACC
AAAGATTCAT TAATAATCAT AGATGAATTG GGAAGAGGAA CTTCTACCTA CGATGGATTT
GGGTTAGCAT GGGCTATATC AGAATACATT GCAACAAAGA TTGGTGCTTT TTGCATGTTT
GCAACCCATT TTCATGAACT TACTGCCTTG GCCAATCAGA TACCAACTGT TAATAATCTA
CATGTCACAG CACTCACCAC TGAAGAGACC TTAACTATGC TTTATCAGGT GAAGAAAGGT
GTCTGTGATC AAAGTTTTGG GATTCATGTT GCAGAGCTTG CTAATTTCCC TAAGCATGTA
ATAGAGTGTG CTAAACAGAA AGCCCTGGAA CTTGAGGAGT TTCAGTATAT TGGAGAATCG
CAAGGATATG ATATCATGGA ACCAGCAGCA AAGAAGTGCT ATCTGGAAAG AGAGCAAGGT
GAAAAAATTA TTCAGGAGTT CCTGTCCAAG GTGAAACAAA TGCCCTTTAC TGAAATGTCA
GAAGAAAACA TCACAATAAA GTTAAAACAG CTAAAAGCTG AAGTAATAGC AAAGAATAAT
AGCTTTGTAA ATGAAATCAT TTCACGAATA AAAGTTACTA CGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Mismatch repair protein hMSH2-hMSH6 recognizes mismatches and forms sliding clamps within a D-loop recombination intermediate
Proc. Natl. Acad. Sci. U.S.A. 111 (3), E316-E325 (2014)
Honda M, Okuno Y, Hengel SR, Martin-Lopez JV, Cook CP, Amunugama R, Soukup RJ, Subramanyam S, Fishel R and Spies M.


book

Tumor mismatch repair immunohistochemistry and DNA MLH1 methylation testing of patients with endometrial cancer diagnosed at age younger than 60 years optimizes triage for population-level germline mismatch repair gene mutation testing
J. Clin. Oncol. 32 (2), 90-100 (2014)
Buchanan DD, Tan YY, Walsh MD, Clendenning M, Metcalf AM, Ferguson K, Arnold ST, Thompson BA, Lose FA, Parsons MT, Walters RJ, Pearson SA, Cummings M, Oehler MK, Blomfield PB, Quinn MA, Kirk JA, Stewart CJ, Obermair A, Young JP, Webb PM and Spurdle AB.


book

Excess of extracolonic non-endometrial multiple primary cancers in MSH2 germline mutation carriers over MLH1
J Surg Oncol 108 (7), 433-437 (2013)
Lin-Hurtubise KM, Yheulon CG, Gagliano RA Jr and Lynch HT.


book

Low expression of MSH2 DNA repair protein is associated with poor prognosis in head and neck squamous cell carcinoma
J Appl Oral Sci 21 (5), 416-421 (2013)
Pereira CS, Oliveira MV, Barros LO, Bandeira GA, Santos SH, Basile JR, Guimaraes AL and De Paula AM.


book

Functional examination of MLH1, MSH2, and MSH6 intronic mutations identified in Danish colorectal cancer patients
BMC Med. Genet. 14, 103 (2013)
Petersen SM, Dandanell M, Rasmussen LJ, Gerdes AM, Krogh LN, Bernstein I, Okkels H, Wikman F, Nielsen FC and Hansen TV.


book

Differential cellular expression of the human MSH2 repair enzyme in small and large intestine
Cancer Res. 55 (22), 5146-5150 (1995)
Wilson TM, Ewel A, Duguid JR, Eble JN, Lescoe MK, Fishel R and Kelley MR.


book

Simultaneous amplification of four DNA repair genes and beta-actin in human lymphocytes by multiplex reverse transcriptase-PCR
Cancer Res. 55 (21), 5025-5029 (1995)
Wei Q, Xu X, Cheng L, Legerski RJ and Ali-Osman F.


book

Isolation of an hMSH2-p160 heterodimer that restores DNA mismatch repair to tumor cells
Science 268 (5219), 1909-1912 (1995)
Drummond JT, Li GM, Longley MJ and Modrich P.


book

Lynch Syndrome
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Kohlmann,W. and Gruber,S.B.


book

Hereditary nonpolyposis colorectal cancer (Lynch syndromes I and II). II. Biomarker studies
Cancer 56 (4), 939-951 (1985)
Lynch,H.T., Schuelke,G.S., Kimberling,W.J., Albano,W.A., Lynch,J.F., Biscone,K.A., Lipkin,M.L., Deschner,E.E., Mikol,Y.B., Sandberg,A.A. et al.


 
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