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MTR cDNA ORF clone, Homo sapiens (human)

Gene Symbol MTR
Entrez Gene ID 4548
Full Name 5-methyltetrahydrofolate-homocysteine methyltransferase
Synonyms HMAG, MS, cblG
General protein information
Preferred Names
methionine synthase
Names
methionine synthase
cobalamin-dependent methionine synthase
vitamin-B12 dependent methionine synthase
5-methyltetrahydrofolate-homocysteine methyltransferase 1
NP_000245.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q43

Summary This gene encodes the 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]. lac of sum
Disorder MIM:

156570

Disorder Html: Methylcobalamin deficiency, cblG type, 250940 (3); {Neural tube

mRNA and Protein(s)

mRNA Protein Name
XM_005273141 XP_005273198 methionine synthase isoform X1
XM_011544193 XP_011542495 methionine synthase isoform X2
XM_006711769 XP_006711832 methionine synthase isoform X3
XM_006711770 XP_006711833 methionine synthase isoform X4
XM_011544194 XP_011542496 methionine synthase isoform X5
NM_000254 NP_000245 methionine synthase isoform 1
NM_001291939 NP_001278868 methionine synthase isoform 2
NM_001291940 NP_001278869 methionine synthase isoform 3



Homo sapiens (human) MTR NP_000245.2
Pan troglodytes (chimpanzee) MTR XP_514294.2
Macaca mulatta (Rhesus monkey) MTR XP_001109238.1
Canis lupus familiaris (dog) MTR XP_005618839.1
Bos taurus (cattle) MTR NP_001025469.1
Mus musculus (house mouse) Mtr NP_001074597.1
Rattus norvegicus (Norway rat) Mtr NP_110491.1
Gallus gallus (chicken) MTR NP_001026275.1
Danio rerio (zebrafish) mtr NP_932338.1
Caenorhabditis elegans metr-1 NP_496353.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100488827 XP_002933914.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MTR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu35026 XM_005273141 PREDICTED: Homo sapiens 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu59094 XM_011544193 PREDICTED: Homo sapiens 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu35027 XM_006711769 PREDICTED: Homo sapiens 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu35028 XM_006711770 PREDICTED: Homo sapiens 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu59095 XM_011544194 PREDICTED: Homo sapiens 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu24754 NM_000254 Homo sapiens 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu25221 NM_001291939 Homo sapiens 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu25092 NM_001291940 Homo sapiens 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
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All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu35026
Clone ID Related Accession (Same CDS sequence) XM_005273141
Accession Version XM_005273141.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3795bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product methionine synthase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167186.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578802219. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGTCACCCG CGCTCCAAGA CCTGTCGCAA CCCGGTCTGA AGAAAACCCT GCGGGATGAG 
ATCAATGCCA TTCTGCAGAA GAGGATTATG GTGCTGGATG GAGGGATGGG GACCATGATC
CAGCGGGAGA AGCTAAACGA AGAACACTTC CGAGGTCAGG AATTTAAAGA TCATGCCAGG
CCGCTGAAAG GCAACAATGA CATTTTAAGT ATAACTCAGC CTGATGTCAT TTACCAAATC
CATAAGGAAT ACTTGCTGGC TGGGGCAGAT ATCATTGAAA CAAATACTTT TAGCAGCACT
AGTATTGCCC AAGCTGACTA TGGCCTTGAA CACTTGGCCT ACCGGATGAA CATGTGCTCT
GCAGGAGTGG CCAGAAAAGC TGCCGAGGAG GTAACTCTCC AGACAGGAAT TAAGAGGTTT
GTGGCAGGGG CTCTGGGTCC GACTAATAAG ACACTCTCTG TGTCCCCATC TGTGGAAAGG
CCGGATTATA GGAACATCAC ATTTGATGAG CTTGTTGAAG CATACCAAGA GCAGGCCAAA
GGACTTCTGG ATGGCGGGGT TGATATCTTA CTCATTGAAA CTATTTTTGA TACTGCCAAT
GCCAAGGCAG CCTTGTTTGC ACTCCAAAAT CTTTTTGAGG AGAAATATGC TCCCCGGCCT
ATCTTTATTT CAGGGACGAT CGTTGATAAA AGTGGGCGGA CTCTTTCCGG ACAGACAGGA
GAGGGATTTG TCATCAGCGT GTCTCATGGA GAACCACTCT GCATTGGATT AAATTGTGCT
TTGGGTGCAG CTGAAATGAG ACCTTTTATT GAAATAATTG GAAAATGTAC AACAGCCTAT
GTCCTCTGTT ATCCCAATGC AGGTCTTCCC AACACCTTTG GTGACTATGA TGAAACGCCT
TCTATGATGG CCAAGCACCT AAAGGATTTT GCTATGGATG GCTTGGTCAA TATAGTTGGA
GGATGCTGTG GGTCAACACC AGATCATATC AGGGAAATTG CTGAAGCTGT GAAAAATTGT
AAGCCTAGAG TTCCACCTGC CACTGCTTTT GAAGGACATA TGTTACTGTC TGGTCTAGAG
CCCTTCAGGA TTGGACCGTA CACCAACTTT GTTAACATTG GAGAGCGCTG TAATGTTGCA
GGATCAAGGA AGTTTGCTAA ACTCATCATG GCAGGAAACT ATGAAGAAGC CTTGTGTGTT
GCCAAAGTGC AGGTGGAAAT GGGAGCCCAG GTGTTGGATG TCAACATGGA TGATGGCATG
CTAGATGGTC CAAGTGCAAT GACCAGATTT TGCAACTTAA TTGCTTCCGA GCCAGACATC
GCAAAGGTAC CTTTGTGCAT CGACTCCTCC AATTTTGCTG TGATTGAAGC TGGGTTAAAG
TGCTGCCAAG GGAAGTGCAT TGTCAATAGC ATTAGTCTGA AGGAAGGAGA GGACGACTTC
TTGGAGAAGG CCAGGAAGAT TAAAAAGTAT GGAGCTGCTA TGGTGGTCAT GGCTTTTGAT
GAAGAAGGAC AGGCAACAGA AACAGACACA AAAATCAGAG TGTGCACCCG GGCCTACCAT
CTGCTTGTGA AAAAACTGGG CTTTAATCCA AATGACATTA TTTTTGACCC TAATATCCTA
ACCATTGGGA CTGGAATGGA GGAACACAAC TTGTATGCCA TTAATTTTAT CCATGCAACA
AAAGTCATTA AAGAAACATT ACCTGGAGCC AGAATAAGTG GAGGTCTTTC CAACTTGTCC
TTCTCCTTCC GAGGAATGGA AGCCATTCGA GAAGCAATGC ATGGGGTTTT CCTTTACCAT
GCAATCAAGT CTGGCATGGA CATGGGGATA GTGAATGCTG GAAACCTCCC TGTGTATGAT
GATATCCATA AGGAACTTCT GCAGCTCTGT GAAGATCTCA TCTGGAATAA AGACCCTGAG
GCCACTGAGA AGCTCTTACG TTATGCCCAG ACTCAAGGCA CAGGAGGGAA GAAAGTCATT
CAGACTGATG AGTGGAGAAA TGGCCCTGTC GAAGAACGCC TTGAGTATGC CCTTGTGAAG
GGCATTGAAA AACATATTAT TGAGGATACT GAGGAAGCCA GGTTAAACCA AAAAAAATAT
CCCCGACCTC TCAATATAAT TGAAGGACCC CTGATGAATG GAATGAAAAT TGTTGGTGAT
CTTTTTGGAG CTGGAAAAAT GTTTCTACCT CAGGTTATAA AGTCAGCCCG GGTTATGAAG
AAGGCTGTTG GCCACCTTAT CCCTTTCATG GAAAAAGAAA GAGAAGAAAC CAGAGTGCTT
AACGGCACAG TAGAAGAAGA GGACCCTTAC CAGGGCACCA TCGTGCTGGC CACTGTTAAA
GGCGACGTGC ACGACATAGG CAAGAACATA GTTGGAGTAG TCCTTGGCTG CAATAATTTC
CGAGTTATTG ATTTAGGAGT CATGACTCCA TGTGATAAGA TACTGAAAGC TGCTCTTGAC
CACAAAGCAG ATATAATTGG CCTGTCAGGA CTCATCACTC CTTCCCTGGA TGAAATGATT
TTTGTTGCCA AGGAAATGGA GAGATTAGCT ATAAGGATTC CATTGTTGAT TGGAGGAGCA
ACCACTTCAA AAACCCACAC AGCAGTTAAA ATAGCTCCGA GATACAGTGC ACCTGTAATC
CATGTCCTGG ACGCGTCCAA GAGTGTGGTG GTGTGTTCCC AGCTGTTAGA TGAAAATCTA
AAGGATGAAT ACTTTGAGGA AATCATGGAA GAATATGAAG ATATTAGACA GGACCATTAT
GAGTCTCTCA AGGAGAGGAG ATACTTACCC TTAAGTCAAG CCAGAAAAAG TGGTTTCCAA
ATGGATTGGC TGTCTGAACC TCACCCAGTG AAGCCCACGT TTATTGGGAC CCAGGTCTTT
GAAGACTATG ACCTGCAGAA GCTGGTGGAC TACATTGACT GGAAGCCTTT CTTTGATGTC
TGGCAGCTCC GGGGCAAGTA CCCGAATCGA GGCTTTCCCA AGATATTTAA CGACAAAACA
GTAGGTGGAG AGGCCAGGAA GGTCTACGAT GATGCCCACA ATATGCTGAA CACACTGATT
AGTCAAAAGA AACTCCGGGC CCGGGGTGTG GTTGGGTTCT GGCCAGCACA GAGTATCCAA
GACGACATTC ACCTGTACGC AGAGGCTGCT GTGCCCCAGG CTGCAGAGCC CATAGCCACC
TTCTATGGGT TAAGGCAACA GGCTGAGAAG GACTCTGCCA GCACGGAGCC ATACTACTGC
CTCTCAGACT TCATCGCTCC CTTGCATTCT GGCATCCGTG ACTACCTGGG CCTGTTTGCC
GTTGCCTGCT TTGGGGTAGA AGAGCTGAGC AAGGCCTATG AGGATGATGG TGACGACTAC
AGCAGCATCA TGGTCAAGGC GCTGGGGGAC CGGCTGGCAG AGGCCTTTGC AGAAGAGCTC
CATGAAAGAG TTCGCCGAGA ACTGTGGGCC TACTGTGGCA GTGAGCAGCT GGACGTCGCA
GACCTGCGCA GGCTGCGGTA CAAGGGCATC CGCCCGGCTC CTGGCTACCC CAGCCAGCCC
GACCACACCG AGAAGCTCAC CATGTGGAGA CTCGCAGACA TCGAGCAGTC TACAGGCATT
AGGTTAACAG AATCATTAGC AATGGCACCT GCTTCAGCAG TCTCAGGCCT CTACTTCTCC
AATTTGAAGT CCAAATATTT TGCTGTGGGG AAGATTTCCA AGGATCAGGT TGAGGATTAT
GCATTGAGGA AGAACATATC TGTGGCTGAG GTTGAGAAAT GGCTTGGACC CATTTTGGGA
TATGATACAG ACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005273198.1
CDS1401..5195
Misc Feature(1)1452..5180(+)
Misc Feature(2)1458..2417(+)
Misc Feature(3)2508..3278(+)
Misc Feature(4)2532..3236(+)
Misc Feature(5)3408..4088(+)
Misc Feature(6)3522..4064(+)
Misc Feature(7)3750..3752(+)
Misc Feature(8)4287..5138(+)
Translation

Target ORF information:

RefSeq Version XM_005273141
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005273141

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGTCACCCG CGCTCCAAGA CCTGTCGCAA CCCGGTCTGA AGAAAACCCT GCGGGATGAG 
ATCAATGCCA TTCTGCAGAA GAGGATTATG GTGCTGGATG GAGGGATGGG GACCATGATC
CAGCGGGAGA AGCTAAACGA AGAACACTTC CGAGGTCAGG AATTTAAAGA TCATGCCAGG
CCGCTGAAAG GCAACAATGA CATTTTAAGT ATAACTCAGC CTGATGTCAT TTACCAAATC
CATAAGGAAT ACTTGCTGGC TGGGGCAGAT ATCATTGAAA CAAATACTTT TAGCAGCACT
AGTATTGCCC AAGCTGACTA TGGCCTTGAA CACTTGGCCT ACCGGATGAA CATGTGCTCT
GCAGGAGTGG CCAGAAAAGC TGCCGAGGAG GTAACTCTCC AGACAGGAAT TAAGAGGTTT
GTGGCAGGGG CTCTGGGTCC GACTAATAAG ACACTCTCTG TGTCCCCATC TGTGGAAAGG
CCGGATTATA GGAACATCAC ATTTGATGAG CTTGTTGAAG CATACCAAGA GCAGGCCAAA
GGACTTCTGG ATGGCGGGGT TGATATCTTA CTCATTGAAA CTATTTTTGA TACTGCCAAT
GCCAAGGCAG CCTTGTTTGC ACTCCAAAAT CTTTTTGAGG AGAAATATGC TCCCCGGCCT
ATCTTTATTT CAGGGACGAT CGTTGATAAA AGTGGGCGGA CTCTTTCCGG ACAGACAGGA
GAGGGATTTG TCATCAGCGT GTCTCATGGA GAACCACTCT GCATTGGATT AAATTGTGCT
TTGGGTGCAG CTGAAATGAG ACCTTTTATT GAAATAATTG GAAAATGTAC AACAGCCTAT
GTCCTCTGTT ATCCCAATGC AGGTCTTCCC AACACCTTTG GTGACTATGA TGAAACGCCT
TCTATGATGG CCAAGCACCT AAAGGATTTT GCTATGGATG GCTTGGTCAA TATAGTTGGA
GGATGCTGTG GGTCAACACC AGATCATATC AGGGAAATTG CTGAAGCTGT GAAAAATTGT
AAGCCTAGAG TTCCACCTGC CACTGCTTTT GAAGGACATA TGTTACTGTC TGGTCTAGAG
CCCTTCAGGA TTGGACCGTA CACCAACTTT GTTAACATTG GAGAGCGCTG TAATGTTGCA
GGATCAAGGA AGTTTGCTAA ACTCATCATG GCAGGAAACT ATGAAGAAGC CTTGTGTGTT
GCCAAAGTGC AGGTGGAAAT GGGAGCCCAG GTGTTGGATG TCAACATGGA TGATGGCATG
CTAGATGGTC CAAGTGCAAT GACCAGATTT TGCAACTTAA TTGCTTCCGA GCCAGACATC
GCAAAGGTAC CTTTGTGCAT CGACTCCTCC AATTTTGCTG TGATTGAAGC TGGGTTAAAG
TGCTGCCAAG GGAAGTGCAT TGTCAATAGC ATTAGTCTGA AGGAAGGAGA GGACGACTTC
TTGGAGAAGG CCAGGAAGAT TAAAAAGTAT GGAGCTGCTA TGGTGGTCAT GGCTTTTGAT
GAAGAAGGAC AGGCAACAGA AACAGACACA AAAATCAGAG TGTGCACCCG GGCCTACCAT
CTGCTTGTGA AAAAACTGGG CTTTAATCCA AATGACATTA TTTTTGACCC TAATATCCTA
ACCATTGGGA CTGGAATGGA GGAACACAAC TTGTATGCCA TTAATTTTAT CCATGCAACA
AAAGTCATTA AAGAAACATT ACCTGGAGCC AGAATAAGTG GAGGTCTTTC CAACTTGTCC
TTCTCCTTCC GAGGAATGGA AGCCATTCGA GAAGCAATGC ATGGGGTTTT CCTTTACCAT
GCAATCAAGT CTGGCATGGA CATGGGGATA GTGAATGCTG GAAACCTCCC TGTGTATGAT
GATATCCATA AGGAACTTCT GCAGCTCTGT GAAGATCTCA TCTGGAATAA AGACCCTGAG
GCCACTGAGA AGCTCTTACG TTATGCCCAG ACTCAAGGCA CAGGAGGGAA GAAAGTCATT
CAGACTGATG AGTGGAGAAA TGGCCCTGTC GAAGAACGCC TTGAGTATGC CCTTGTGAAG
GGCATTGAAA AACATATTAT TGAGGATACT GAGGAAGCCA GGTTAAACCA AAAAAAATAT
CCCCGACCTC TCAATATAAT TGAAGGACCC CTGATGAATG GAATGAAAAT TGTTGGTGAT
CTTTTTGGAG CTGGAAAAAT GTTTCTACCT CAGGTTATAA AGTCAGCCCG GGTTATGAAG
AAGGCTGTTG GCCACCTTAT CCCTTTCATG GAAAAAGAAA GAGAAGAAAC CAGAGTGCTT
AACGGCACAG TAGAAGAAGA GGACCCTTAC CAGGGCACCA TCGTGCTGGC CACTGTTAAA
GGCGACGTGC ACGACATAGG CAAGAACATA GTTGGAGTAG TCCTTGGCTG CAATAATTTC
CGAGTTATTG ATTTAGGAGT CATGACTCCA TGTGATAAGA TACTGAAAGC TGCTCTTGAC
CACAAAGCAG ATATAATTGG CCTGTCAGGA CTCATCACTC CTTCCCTGGA TGAAATGATT
TTTGTTGCCA AGGAAATGGA GAGATTAGCT ATAAGGATTC CATTGTTGAT TGGAGGAGCA
ACCACTTCAA AAACCCACAC AGCAGTTAAA ATAGCTCCGA GATACAGTGC ACCTGTAATC
CATGTCCTGG ACGCGTCCAA GAGTGTGGTG GTGTGTTCCC AGCTGTTAGA TGAAAATCTA
AAGGATGAAT ACTTTGAGGA AATCATGGAA GAATATGAAG ATATTAGACA GGACCATTAT
GAGTCTCTCA AGGAGAGGAG ATACTTACCC TTAAGTCAAG CCAGAAAAAG TGGTTTCCAA
ATGGATTGGC TGTCTGAACC TCACCCAGTG AAGCCCACGT TTATTGGGAC CCAGGTCTTT
GAAGACTATG ACCTGCAGAA GCTGGTGGAC TACATTGACT GGAAGCCTTT CTTTGATGTC
TGGCAGCTCC GGGGCAAGTA CCCGAATCGA GGCTTTCCCA AGATATTTAA CGACAAAACA
GTAGGTGGAG AGGCCAGGAA GGTCTACGAT GATGCCCACA ATATGCTGAA CACACTGATT
AGTCAAAAGA AACTCCGGGC CCGGGGTGTG GTTGGGTTCT GGCCAGCACA GAGTATCCAA
GACGACATTC ACCTGTACGC AGAGGCTGCT GTGCCCCAGG CTGCAGAGCC CATAGCCACC
TTCTATGGGT TAAGGCAACA GGCTGAGAAG GACTCTGCCA GCACGGAGCC ATACTACTGC
CTCTCAGACT TCATCGCTCC CTTGCATTCT GGCATCCGTG ACTACCTGGG CCTGTTTGCC
GTTGCCTGCT TTGGGGTAGA AGAGCTGAGC AAGGCCTATG AGGATGATGG TGACGACTAC
AGCAGCATCA TGGTCAAGGC GCTGGGGGAC CGGCTGGCAG AGGCCTTTGC AGAAGAGCTC
CATGAAAGAG TTCGCCGAGA ACTGTGGGCC TACTGTGGCA GTGAGCAGCT GGACGTCGCA
GACCTGCGCA GGCTGCGGTA CAAGGGCATC CGCCCGGCTC CTGGCTACCC CAGCCAGCCC
GACCACACCG AGAAGCTCAC CATGTGGAGA CTCGCAGACA TCGAGCAGTC TACAGGCATT
AGGTTAACAG AATCATTAGC AATGGCACCT GCTTCAGCAG TCTCAGGCCT CTACTTCTCC
AATTTGAAGT CCAAATATTT TGCTGTGGGG AAGATTTCCA AGGATCAGGT TGAGGATTAT
GCATTGAGGA AGAACATATC TGTGGCTGAG GTTGAGAAAT GGCTTGGACC CATTTTGGGA
TATGATACAG ACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59094
Clone ID Related Accession (Same CDS sequence) XM_011544193
Accession Version XM_011544193.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3609bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product methionine synthase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167186.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGTCACCCG CGCTCCAAGA CCTGTCGCAA CCCGAAGGTC TGAAGAAAAC CCTGCGGGAT 
GAGATCAATG CCATTCTGCA GAAGAGGATT ATGGTGCTGG ATGGAGGGAT GGGGACCATG
ATCCAGCGGG AGAAGCTAAA CGAAGAACAC TTCCGAGGTC AGGAATTTAA AGATCATGCC
AGGCCGCTGA AAGGCAACAA TGACATTTTA AGTATAACTC AGCCTGATGT CATTTACCAA
ATCCATAAGG AATACTTGCT GGCTGGGGCA GATATCATTG AAACAAATAC TTTTAGCAGC
ACTAGTATTG CCCAAGCTGA CTATGGCCTT GAACACTTGG CCTACCGGAT GAACATGTGC
TCTGCAGGAG TGGCCAGAAA AGCTGCCGAG GAGGTAACTC TCCAGACAGG AATTAAGAGG
TTTGTGGCAG GGGCTCTGGG TCCGACTAAT AAGACACTCT CTGTGTCCCC ATCTGTGGAA
AGGCCGGATT ATAGGAACAT CACATTTGAT GAGCTTGTTG AAGCATACCA AGAGCAGGCC
AAAGGACTTC TGGATGGCGG GGTTGATATC TTACTCATTG AAACTATTTT TGATACTGCC
AATGCCAAGG CAGCCTTGTT TGCACTCCAA AATCTTTTTG AGGAGAAATA TGCTCCCCGG
CCTATCTTTA TTTCAGGGAC GATCGTTGAT AAAAGTGGGC GGACTCTTTC CGGACAGACA
GGAGAGGGAT TTGTCATCAG CGTGTCTCAT GGAGAACCAC TCTGCATTGG ATTAAATTGT
GCTTTGGGTG CAGCTGAAAT GAGACCTTTT ATTGAAATAA TTGGAAAATG TACAACAGCC
TATGTCCTCT GTTATCCCAA TGCAGGTCTT CCCAACACCT TTGGTGACTA TGATGAAACG
CCTTCTATGA TGGCCAAGCA CCTAAAGGAT TTTGCTATGG ATGGCTTGGT CAATATAGTT
GGAGGATGCT GTGGGTCAAC ACCAGATCAT ATCAGGGAAA TTGCTGAAGC TGTGAAAAAT
TGTAAGCCTA GAGTTCCACC TGCCACTGCT TTTGAAGGAC ATATGTTACT GTCTGGTCTA
GAGCCCTTCA GGATTGGACC GTACACCAAC TTTGTTAACA TTGGAGAGCG CTGTAATGTT
GCAGGATCAA GGAAGTTTGC TAAACTCATC ATGGCAGGAA ACTATGAAGA AGCCTTGTGT
GTTGCCAAAG TGCAGGTGGA AATGGGAGCC CAGGTGTTGG ATGTCAACAT GGATGATGGC
ATGCTAGATG GTCCAAGTGC AATGACCAGA TTTTGCAACT TAATTGCTTC CGAGCCAGAC
ATCGCAAAGG TACCTTTGTG CATCGACTCC TCCAATTTTG CTGTGATTGA AGCTGGGTTA
AAGTGCTGCC AAGGGAAGTG CATTGTCAAT AGCATTAGTC TGAAGGAAGG AGAGGACGAC
TTCTTGGAGA AGGCCAGGAA GATTAAAAAG TATGGAGCTG CTATGGTGGT CATGGCTTTT
GATGAAGAAG GACAGGCAAC AGAAACAGAC ACAAAAATCA GAGTGTGCAC CCGGGCCTAC
CATCTGCTTG TGAAAAAACT GGGCTTTAAT CCAAATGACA TTATTTTTGA CCCTAATATC
CTAACCATTG GGACTGGAAT GGAGGAACAC AACTTGTATG CCATTAATTT TATCCATGCA
ACAAAAGTCA TTAAAGAAAC ATTACCTGGA GCCAGAATAA GTGGAGGTCT TTCCAACTTG
TCCTTCTCCT TCCGAGGAAT GGAAGCCATT CGAGAAGCAA TGCATGGGGT TTTCCTTTAC
CATGCAATCA AGTCTGGCAT GGACATGGGG ATAGTGAATG CTGGAAACCT CCCTGTGTAT
GATGATATCC ATAAGGAACT TCTGCAGCTC TGTGAAGATC TCATCTGGAA TAAAGACCCT
GAGGCCACTG AGAAGCTCTT ACGTTATGCC CAGACTCAAG GCACAGGAGG GAAGAAAGTC
ATTCAGACTG ATGAGTGGAG AAATGGCCCT GTCGAAGAAC GCCTTGAGTA TGCCCTTGTG
AAGGGCATTG AAAAACATAT TATTGAGGAT ACTGAGGAAG CCAGGTTAAA CCAAAAAAAA
TATCCCCGAC CTCTCAATAT AATTGAAGGA CCCCTGATGA ATGGAATGAA AATTGTTGGT
GATCTTTTTG GAGCTGGAAA AATGTTTCTA CCTCAGGTTA TAAAGTCAGC CCGGGTTATG
AAGAAGGCTG TTGGCCACCT TATCCCTTTC ATGGAAAAAG AAAGAGAAGA AACCAGAGTG
CTTAACGGCA CAGTAGAAGA AGAGGACCCT TACCAGGGCA CCATCGTGCT GGCCACTGTT
AAAGGCGACG TGCACGACAT AGGCAAGAAC ATAGTTGGAG TAGTCCTTGG CTGCAATAAT
TTCCGAACCC ACACAGCAGT TAAAATAGCT CCGAGATACA GTGCACCTGT AATCCATGTC
CTGGACGCGT CCAAGAGTGT GGTGGTGTGT TCCCAGCTGT TAGATGAAAA TCTAAAGGAT
GAATACTTTG AGGAAATCAT GGAAGAATAT GAAGATATTA GACAGGACCA TTATGAGTCT
CTCAAGGAGA GGAGATACTT ACCCTTAAGT CAAGCCAGAA AAAGTGGTTT CCAAATGGAT
TGGCTGTCTG AACCTCACCC AGTGAAGCCC ACGTTTATTG GGACCCAGGT CTTTGAAGAC
TATGACCTGC AGAAGCTGGT GGACTACATT GACTGGAAGC CTTTCTTTGA TGTCTGGCAG
CTCCGGGGCA AGTACCCGAA TCGAGGCTTT CCCAAGATAT TTAACGACAA AACAGTAGGT
GGAGAGGCCA GGAAGGTCTA CGATGATGCC CACAATATGC TGAACACACT GATTAGTCAA
AAGAAACTCC GGGCCCGGGG TGTGGTTGGG TTCTGGCCAG CACAGAGTAT CCAAGACGAC
ATTCACCTGT ACGCAGAGGC TGCTGTGCCC CAGGCTGCAG AGCCCATAGC CACCTTCTAT
GGGTTAAGGC AACAGGCTGA GAAGGACTCT GCCAGCACGG AGCCATACTA CTGCCTCTCA
GACTTCATCG CTCCCTTGCA TTCTGGCATC CGTGACTACC TGGGCCTGTT TGCCGTTGCC
TGCTTTGGGG TAGAAGAGCT GAGCAAGGCC TATGAGGATG ATGGTGACGA CTACAGCAGC
ATCATGGTCA AGGCGCTGGG GGACCGGCTG GCAGAGGCCT TTGCAGAAGA GCTCCATGAA
AGAGTTCGCC GAGAACTGTG GGCCTACTGT GGCAGTGAGC AGCTGGACGT CGCAGACCTG
CGCAGGCTGC GGTACAAGGG CATCCGCCCG GCTCCTGGCT ACCCCAGCCA GCCCGACCAC
ACCGAGAAGC TCACCATGTG GAGACTCGCA GACATCGAGC AGTCTACAGG CATTAGGTTA
ACAGAATCAT TAGCAATGGC ACCTGCTTCA GCAGTCTCAG GCCTCTACTT CTCCAATTTG
AAGTCCAAAT ATTTTGCTGT GGGGAAGATT TCCAAGGATC AGGTTGAGGA TTATGCATTG
AGGAAGAACA TATCTGTGGC TGAGGTTGAG AAATGGCTTG GACCCATTTT GGGATATGAT
ACAGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542495.1
CDS1401..5009
Misc Feature(1)1455..4994(+)
Misc Feature(2)1461..2420(+)
Misc Feature(3)2511..3281(+)
Misc Feature(4)2535..3239(+)
Misc Feature(5)3411..3902(+)
Misc Feature(6)4101..4952(+)
Translation

Target ORF information:

RefSeq Version XM_011544193
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011544193

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGTCACCCG CGCTCCAAGA CCTGTCGCAA CCCGAAGGTC TGAAGAAAAC CCTGCGGGAT 
GAGATCAATG CCATTCTGCA GAAGAGGATT ATGGTGCTGG ATGGAGGGAT GGGGACCATG
ATCCAGCGGG AGAAGCTAAA CGAAGAACAC TTCCGAGGTC AGGAATTTAA AGATCATGCC
AGGCCGCTGA AAGGCAACAA TGACATTTTA AGTATAACTC AGCCTGATGT CATTTACCAA
ATCCATAAGG AATACTTGCT GGCTGGGGCA GATATCATTG AAACAAATAC TTTTAGCAGC
ACTAGTATTG CCCAAGCTGA CTATGGCCTT GAACACTTGG CCTACCGGAT GAACATGTGC
TCTGCAGGAG TGGCCAGAAA AGCTGCCGAG GAGGTAACTC TCCAGACAGG AATTAAGAGG
TTTGTGGCAG GGGCTCTGGG TCCGACTAAT AAGACACTCT CTGTGTCCCC ATCTGTGGAA
AGGCCGGATT ATAGGAACAT CACATTTGAT GAGCTTGTTG AAGCATACCA AGAGCAGGCC
AAAGGACTTC TGGATGGCGG GGTTGATATC TTACTCATTG AAACTATTTT TGATACTGCC
AATGCCAAGG CAGCCTTGTT TGCACTCCAA AATCTTTTTG AGGAGAAATA TGCTCCCCGG
CCTATCTTTA TTTCAGGGAC GATCGTTGAT AAAAGTGGGC GGACTCTTTC CGGACAGACA
GGAGAGGGAT TTGTCATCAG CGTGTCTCAT GGAGAACCAC TCTGCATTGG ATTAAATTGT
GCTTTGGGTG CAGCTGAAAT GAGACCTTTT ATTGAAATAA TTGGAAAATG TACAACAGCC
TATGTCCTCT GTTATCCCAA TGCAGGTCTT CCCAACACCT TTGGTGACTA TGATGAAACG
CCTTCTATGA TGGCCAAGCA CCTAAAGGAT TTTGCTATGG ATGGCTTGGT CAATATAGTT
GGAGGATGCT GTGGGTCAAC ACCAGATCAT ATCAGGGAAA TTGCTGAAGC TGTGAAAAAT
TGTAAGCCTA GAGTTCCACC TGCCACTGCT TTTGAAGGAC ATATGTTACT GTCTGGTCTA
GAGCCCTTCA GGATTGGACC GTACACCAAC TTTGTTAACA TTGGAGAGCG CTGTAATGTT
GCAGGATCAA GGAAGTTTGC TAAACTCATC ATGGCAGGAA ACTATGAAGA AGCCTTGTGT
GTTGCCAAAG TGCAGGTGGA AATGGGAGCC CAGGTGTTGG ATGTCAACAT GGATGATGGC
ATGCTAGATG GTCCAAGTGC AATGACCAGA TTTTGCAACT TAATTGCTTC CGAGCCAGAC
ATCGCAAAGG TACCTTTGTG CATCGACTCC TCCAATTTTG CTGTGATTGA AGCTGGGTTA
AAGTGCTGCC AAGGGAAGTG CATTGTCAAT AGCATTAGTC TGAAGGAAGG AGAGGACGAC
TTCTTGGAGA AGGCCAGGAA GATTAAAAAG TATGGAGCTG CTATGGTGGT CATGGCTTTT
GATGAAGAAG GACAGGCAAC AGAAACAGAC ACAAAAATCA GAGTGTGCAC CCGGGCCTAC
CATCTGCTTG TGAAAAAACT GGGCTTTAAT CCAAATGACA TTATTTTTGA CCCTAATATC
CTAACCATTG GGACTGGAAT GGAGGAACAC AACTTGTATG CCATTAATTT TATCCATGCA
ACAAAAGTCA TTAAAGAAAC ATTACCTGGA GCCAGAATAA GTGGAGGTCT TTCCAACTTG
TCCTTCTCCT TCCGAGGAAT GGAAGCCATT CGAGAAGCAA TGCATGGGGT TTTCCTTTAC
CATGCAATCA AGTCTGGCAT GGACATGGGG ATAGTGAATG CTGGAAACCT CCCTGTGTAT
GATGATATCC ATAAGGAACT TCTGCAGCTC TGTGAAGATC TCATCTGGAA TAAAGACCCT
GAGGCCACTG AGAAGCTCTT ACGTTATGCC CAGACTCAAG GCACAGGAGG GAAGAAAGTC
ATTCAGACTG ATGAGTGGAG AAATGGCCCT GTCGAAGAAC GCCTTGAGTA TGCCCTTGTG
AAGGGCATTG AAAAACATAT TATTGAGGAT ACTGAGGAAG CCAGGTTAAA CCAAAAAAAA
TATCCCCGAC CTCTCAATAT AATTGAAGGA CCCCTGATGA ATGGAATGAA AATTGTTGGT
GATCTTTTTG GAGCTGGAAA AATGTTTCTA CCTCAGGTTA TAAAGTCAGC CCGGGTTATG
AAGAAGGCTG TTGGCCACCT TATCCCTTTC ATGGAAAAAG AAAGAGAAGA AACCAGAGTG
CTTAACGGCA CAGTAGAAGA AGAGGACCCT TACCAGGGCA CCATCGTGCT GGCCACTGTT
AAAGGCGACG TGCACGACAT AGGCAAGAAC ATAGTTGGAG TAGTCCTTGG CTGCAATAAT
TTCCGAACCC ACACAGCAGT TAAAATAGCT CCGAGATACA GTGCACCTGT AATCCATGTC
CTGGACGCGT CCAAGAGTGT GGTGGTGTGT TCCCAGCTGT TAGATGAAAA TCTAAAGGAT
GAATACTTTG AGGAAATCAT GGAAGAATAT GAAGATATTA GACAGGACCA TTATGAGTCT
CTCAAGGAGA GGAGATACTT ACCCTTAAGT CAAGCCAGAA AAAGTGGTTT CCAAATGGAT
TGGCTGTCTG AACCTCACCC AGTGAAGCCC ACGTTTATTG GGACCCAGGT CTTTGAAGAC
TATGACCTGC AGAAGCTGGT GGACTACATT GACTGGAAGC CTTTCTTTGA TGTCTGGCAG
CTCCGGGGCA AGTACCCGAA TCGAGGCTTT CCCAAGATAT TTAACGACAA AACAGTAGGT
GGAGAGGCCA GGAAGGTCTA CGATGATGCC CACAATATGC TGAACACACT GATTAGTCAA
AAGAAACTCC GGGCCCGGGG TGTGGTTGGG TTCTGGCCAG CACAGAGTAT CCAAGACGAC
ATTCACCTGT ACGCAGAGGC TGCTGTGCCC CAGGCTGCAG AGCCCATAGC CACCTTCTAT
GGGTTAAGGC AACAGGCTGA GAAGGACTCT GCCAGCACGG AGCCATACTA CTGCCTCTCA
GACTTCATCG CTCCCTTGCA TTCTGGCATC CGTGACTACC TGGGCCTGTT TGCCGTTGCC
TGCTTTGGGG TAGAAGAGCT GAGCAAGGCC TATGAGGATG ATGGTGACGA CTACAGCAGC
ATCATGGTCA AGGCGCTGGG GGACCGGCTG GCAGAGGCCT TTGCAGAAGA GCTCCATGAA
AGAGTTCGCC GAGAACTGTG GGCCTACTGT GGCAGTGAGC AGCTGGACGT CGCAGACCTG
CGCAGGCTGC GGTACAAGGG CATCCGCCCG GCTCCTGGCT ACCCCAGCCA GCCCGACCAC
ACCGAGAAGC TCACCATGTG GAGACTCGCA GACATCGAGC AGTCTACAGG CATTAGGTTA
ACAGAATCAT TAGCAATGGC ACCTGCTTCA GCAGTCTCAG GCCTCTACTT CTCCAATTTG
AAGTCCAAAT ATTTTGCTGT GGGGAAGATT TCCAAGGATC AGGTTGAGGA TTATGCATTG
AGGAAGAACA TATCTGTGGC TGAGGTTGAG AAATGGCTTG GACCCATTTT GGGATATGAT
ACAGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu35027
Clone ID Related Accession (Same CDS sequence) XM_006711769
Accession Version XM_006711769.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3420bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product methionine synthase isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167186.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578802221. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGTCACCCG CGCTCCAAGA CCTGTCGCAA CCCGAAGGTC TGAAGAAAAC CCTGCGGGAT 
GAGATCAATG CCATTCTGCA GAAGAGGATT ATGGTGCTGG ATGGAGGGAT GGGGACCATG
ATCCAGCGGG AGAAGCTAAA CGAAGAACAC TTCCGAGGTC AGGAATTTAA AGATCATGCC
AGGCCGCTGA AAGGCAACAA TGACATTTTA AGTATAACTC AGCCTGATGT CATTTACCAA
ATCCATAAGG AATACTTGCT GGCTGGGGCA GATATCATTG AAACAAATAC TTTTAGCAGC
ACTAGTATTG CCCAAGCTGA CTATGGCCTT GAACACTTGG CCTACCGGAT GAACATGTGC
TCTGCAGGAG TGGCCAGAAA AGCTGCCGAG GAGGTAACTC TCCAGACAGG AATTAAGAGG
TTTGTGGCAG GGGCTCTGGG TCCGACTAAT AAGACACTCT CTGTGTCCCC ATCTGTGGAA
AGGCCGGATT ATAGGAACAT CACATTTGAT GAGCTTGTTG AAGCATACCA AGAGCAGGCC
AAAGGACTTC TGGATGGCGG GGTTGATATC TTACTCATTG AAACTATTTT TGATACTGCC
AATGCCAAGG CAGCCTTGTT TGCACTCCAA AATCTTTTTG AGGAGAAATA TGCTCCCCGG
CCTATCTTTA TTTCAGGGAC GATCGTTGAT AAAAGTGGGC GGACTCTTTC CGGACAGACA
GGAGAGGGAT TTGTCATCAG CGTGTCTCAT GGAGAACCAC TCTGCATTGG ATTAAATTGT
GCTTTGGGTG CAGCTGAAAT GAGACCTTTT ATTGAAATAA TTGGAAAATG TACAACAGCC
TATGTCCTCT GTTATCCCAA TGCAGGTCTT CCCAACACCT TTGGTGACTA TGATGAAACG
CCTTCTATGA TGGCCAAGCA CCTAAAGGAT TTTGCTATGG ATGGCTTGGT CAATATAGTT
GGAGGATGCT GTGGGTCAAC ACCAGATCAT ATCAGGGAAA TTGCTGAAGC TGTGAAAAAT
TGTAAGCCTA GAGTTCCACC TGCCACTGCT TTTGAAGGAC ATATGTTACT GTCTGGTCTA
GAGCCCTTCA GGATTGGACC GTACACCAAC TTTGTTAACA TTGGAGAGCG CTGTAATGTT
GCAGGATCAA GGAAGTTTGC TAAACTCATC ATGGCAGGAA ACTATGAAGA AGCCTTGTGT
GTTGCCAAAG TGCAGGTGGA AATGGGAGCC CAGGTGTTGG ATGTCAACAT GGATGATGGC
ATGCTAGATG GTCCAAGTGC AATGACCAGA TTTTGCAACT TAATTGCTTC CGAGCCAGAC
ATCGCAAAGG TACCTTTGTG CATCGACTCC TCCAATTTTG CTGTGATTGA AGCTGGGTTA
AAGTGCTGCC AAGGGAAGTG CATTGTCAAT AGCATTAGTC TGAAGGAAGG AGAGGACGAC
TTCTTGGAGA AGGCCAGGAA GATTAAAAAG TATGGAGCTG CTATGGTGGT CATGGCTTTT
GATGAAGAAG GACAGGCAAC AGAAACAGAC ACAAAAATCA GAGTGTGCAC CCGGGCCTAC
CATCTGCTTG TGAAAAAACT GGGCTTTAAT CCAAATGACA TTATTTTTGA CCCTAATATC
CTAACCATTG GGACTGGAAT GGAGGAACAC AACTTGTATG CCATTAATTT TATCCATGCA
ACAAAAGTCA TTAAAGAAAC ATTACCTGGA GCCAGAATAA GTGGAGGTCT TTCCAACTTG
TCCTTCTCCT TCCGAGGAAT GGAAGCCATT CGAGAAGCAA TGCATGGGGT TTTCCTTTAC
CATGCAATCA AGTCTGGCAT GGACATGGGG ATAGTGAATG CTGGAAACCT CCCTGTGTAT
GATGATATCC ATAAGGAACT TCTGCAGCTC TGTGAAGATC TCATCTGGAA TAAAGACCCT
GAGGCCACTG AGAAGCTCTT ACGTTATGCC CAGACTCAAG GCACAGGAGG GAAGAAAGTC
ATTCAGACTG ATGAGTGGAG AAATGGCCCT GTCGAAGAAC GCCTTGAGTA TGCCCTTGTG
AAGGGCATTG AAAAACATAT TATTGAGGAT ACTGAGGAAG CCAGGTTAAA CCAAAAAAAA
TATCCCCGAC CTCTCAATAT AATTGAAGGA CCCCTGATGA ATGGAATGAA AATTGTTGGT
GATCTTTTTG GAGCTGGAAA AATGTTTCTA CCTCAGGTTA TAAAGTCAGC CCGGGTTATG
AAGAAGGCTG TTGGCCACCT TATCCCTTTC ATGGAAAAAG AAAGAGAAGA AACCAGAGTG
CTTAACGGCA CAGTAGAAGA AGAGGACCCT TACCAGGGCA CCATCGTGCT GGCCACTGTT
AAAGGCGACG TGCACGACAT AGGCAAGAAC ATAGTTGGAG TAGTCCTTGG CTGCAATAAT
TTCCGAGTTA TTGATTTAGG AGTCATGACT CCATGTGATA AGATACTGAA AGCTGCTCTT
GACCACAAAG CAGATATAAT TGGCCTGTCA GGACTCATCA CTCCTTCCCT GGATGAAATG
ATTTTTGTTG CCAAGGAAAT GGAGAGATTA GCTATAAGGA TTCCATTGTT GATTGGAGGA
GCAACCACTT CAAAAACCCA CACAGCAGTT AAAATAGCTC CGAGATACAG TGCACCTGTA
ATCCATGTCC TGGACGCGTC CAAGAGTGTG GTGGTGTGTT CCCAGCTGTT AGATGAAAAT
CTAAAGGATG AATACTTTGA GGAAATCATG GAAGAATATG AAGATATTAG ACAGGACCAT
TATGAGTCTC TCAAGGAGAG GAGATACTTA CCCTTAAGTC AAGCCAGAAA AAGTGGTTTC
CAAATGGATT GGCTGTCTGA ACCTCACCCA GTGAAGCCCA CGTTTATTGG GACCCAGGTC
TTTGAAGACT ATGACCTGCA GAAGCTGGTG GACTACATTG ACTGGAAGCC TTTCTTTGAT
GTCTGGCAGC TCCGGGGCAA GTACCCGAAT CGAGGCTTTC CCAAGATATT TAACGACAAA
ACAGTAGGTG GAGAGGCCAG GAAGGTCTAC GATGATGCCC ACAATATGCT GAACACACTG
ATTAGTCAAA AGAAACTCCG GGCCCGGGGT GTGGTTGGGT TCTGGCCAGC ACAGAGTATC
CAAGACGACA TTCACCTGTA CGCAGAGGCT GCTGTGCCCC AGGCTGCAGA GCCCATAGCC
ACCTTCTATG GGTTAAGGCA ACAGGCTGAG AAGGACTCTG CCAGCACGGA GCCATACTAC
TGCCTCTCAG ACTTCATCGC TCCCTTGCAT TCTGGCATCC GTGACTACCT GGGCCTGTTT
GCCGTTGCCT GCTTTGGGGT AGAAGAGCTG AGCAAGGCCT ATGAGGATGA TGGTGACGAC
TACAGCAGCA TCATGGTCAA GGCGCTGGGG GACCGGCTGG CAGAGAGCCC CTCGTTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006711832.1
CDS1401..4820
Misc Feature(1)1461..2420(+)
Misc Feature(2)1476..4808(+)
Misc Feature(3)2511..3281(+)
Misc Feature(4)2535..3239(+)
Misc Feature(5)3411..4091(+)
Misc Feature(6)3525..4067(+)
Misc Feature(7)3753..3755(+)
Misc Feature(8)4290..>4805(+)
Translation

Target ORF information:

RefSeq Version XM_006711769
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006711769

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGTCACCCG CGCTCCAAGA CCTGTCGCAA CCCGAAGGTC TGAAGAAAAC CCTGCGGGAT 
GAGATCAATG CCATTCTGCA GAAGAGGATT ATGGTGCTGG ATGGAGGGAT GGGGACCATG
ATCCAGCGGG AGAAGCTAAA CGAAGAACAC TTCCGAGGTC AGGAATTTAA AGATCATGCC
AGGCCGCTGA AAGGCAACAA TGACATTTTA AGTATAACTC AGCCTGATGT CATTTACCAA
ATCCATAAGG AATACTTGCT GGCTGGGGCA GATATCATTG AAACAAATAC TTTTAGCAGC
ACTAGTATTG CCCAAGCTGA CTATGGCCTT GAACACTTGG CCTACCGGAT GAACATGTGC
TCTGCAGGAG TGGCCAGAAA AGCTGCCGAG GAGGTAACTC TCCAGACAGG AATTAAGAGG
TTTGTGGCAG GGGCTCTGGG TCCGACTAAT AAGACACTCT CTGTGTCCCC ATCTGTGGAA
AGGCCGGATT ATAGGAACAT CACATTTGAT GAGCTTGTTG AAGCATACCA AGAGCAGGCC
AAAGGACTTC TGGATGGCGG GGTTGATATC TTACTCATTG AAACTATTTT TGATACTGCC
AATGCCAAGG CAGCCTTGTT TGCACTCCAA AATCTTTTTG AGGAGAAATA TGCTCCCCGG
CCTATCTTTA TTTCAGGGAC GATCGTTGAT AAAAGTGGGC GGACTCTTTC CGGACAGACA
GGAGAGGGAT TTGTCATCAG CGTGTCTCAT GGAGAACCAC TCTGCATTGG ATTAAATTGT
GCTTTGGGTG CAGCTGAAAT GAGACCTTTT ATTGAAATAA TTGGAAAATG TACAACAGCC
TATGTCCTCT GTTATCCCAA TGCAGGTCTT CCCAACACCT TTGGTGACTA TGATGAAACG
CCTTCTATGA TGGCCAAGCA CCTAAAGGAT TTTGCTATGG ATGGCTTGGT CAATATAGTT
GGAGGATGCT GTGGGTCAAC ACCAGATCAT ATCAGGGAAA TTGCTGAAGC TGTGAAAAAT
TGTAAGCCTA GAGTTCCACC TGCCACTGCT TTTGAAGGAC ATATGTTACT GTCTGGTCTA
GAGCCCTTCA GGATTGGACC GTACACCAAC TTTGTTAACA TTGGAGAGCG CTGTAATGTT
GCAGGATCAA GGAAGTTTGC TAAACTCATC ATGGCAGGAA ACTATGAAGA AGCCTTGTGT
GTTGCCAAAG TGCAGGTGGA AATGGGAGCC CAGGTGTTGG ATGTCAACAT GGATGATGGC
ATGCTAGATG GTCCAAGTGC AATGACCAGA TTTTGCAACT TAATTGCTTC CGAGCCAGAC
ATCGCAAAGG TACCTTTGTG CATCGACTCC TCCAATTTTG CTGTGATTGA AGCTGGGTTA
AAGTGCTGCC AAGGGAAGTG CATTGTCAAT AGCATTAGTC TGAAGGAAGG AGAGGACGAC
TTCTTGGAGA AGGCCAGGAA GATTAAAAAG TATGGAGCTG CTATGGTGGT CATGGCTTTT
GATGAAGAAG GACAGGCAAC AGAAACAGAC ACAAAAATCA GAGTGTGCAC CCGGGCCTAC
CATCTGCTTG TGAAAAAACT GGGCTTTAAT CCAAATGACA TTATTTTTGA CCCTAATATC
CTAACCATTG GGACTGGAAT GGAGGAACAC AACTTGTATG CCATTAATTT TATCCATGCA
ACAAAAGTCA TTAAAGAAAC ATTACCTGGA GCCAGAATAA GTGGAGGTCT TTCCAACTTG
TCCTTCTCCT TCCGAGGAAT GGAAGCCATT CGAGAAGCAA TGCATGGGGT TTTCCTTTAC
CATGCAATCA AGTCTGGCAT GGACATGGGG ATAGTGAATG CTGGAAACCT CCCTGTGTAT
GATGATATCC ATAAGGAACT TCTGCAGCTC TGTGAAGATC TCATCTGGAA TAAAGACCCT
GAGGCCACTG AGAAGCTCTT ACGTTATGCC CAGACTCAAG GCACAGGAGG GAAGAAAGTC
ATTCAGACTG ATGAGTGGAG AAATGGCCCT GTCGAAGAAC GCCTTGAGTA TGCCCTTGTG
AAGGGCATTG AAAAACATAT TATTGAGGAT ACTGAGGAAG CCAGGTTAAA CCAAAAAAAA
TATCCCCGAC CTCTCAATAT AATTGAAGGA CCCCTGATGA ATGGAATGAA AATTGTTGGT
GATCTTTTTG GAGCTGGAAA AATGTTTCTA CCTCAGGTTA TAAAGTCAGC CCGGGTTATG
AAGAAGGCTG TTGGCCACCT TATCCCTTTC ATGGAAAAAG AAAGAGAAGA AACCAGAGTG
CTTAACGGCA CAGTAGAAGA AGAGGACCCT TACCAGGGCA CCATCGTGCT GGCCACTGTT
AAAGGCGACG TGCACGACAT AGGCAAGAAC ATAGTTGGAG TAGTCCTTGG CTGCAATAAT
TTCCGAGTTA TTGATTTAGG AGTCATGACT CCATGTGATA AGATACTGAA AGCTGCTCTT
GACCACAAAG CAGATATAAT TGGCCTGTCA GGACTCATCA CTCCTTCCCT GGATGAAATG
ATTTTTGTTG CCAAGGAAAT GGAGAGATTA GCTATAAGGA TTCCATTGTT GATTGGAGGA
GCAACCACTT CAAAAACCCA CACAGCAGTT AAAATAGCTC CGAGATACAG TGCACCTGTA
ATCCATGTCC TGGACGCGTC CAAGAGTGTG GTGGTGTGTT CCCAGCTGTT AGATGAAAAT
CTAAAGGATG AATACTTTGA GGAAATCATG GAAGAATATG AAGATATTAG ACAGGACCAT
TATGAGTCTC TCAAGGAGAG GAGATACTTA CCCTTAAGTC AAGCCAGAAA AAGTGGTTTC
CAAATGGATT GGCTGTCTGA ACCTCACCCA GTGAAGCCCA CGTTTATTGG GACCCAGGTC
TTTGAAGACT ATGACCTGCA GAAGCTGGTG GACTACATTG ACTGGAAGCC TTTCTTTGAT
GTCTGGCAGC TCCGGGGCAA GTACCCGAAT CGAGGCTTTC CCAAGATATT TAACGACAAA
ACAGTAGGTG GAGAGGCCAG GAAGGTCTAC GATGATGCCC ACAATATGCT GAACACACTG
ATTAGTCAAA AGAAACTCCG GGCCCGGGGT GTGGTTGGGT TCTGGCCAGC ACAGAGTATC
CAAGACGACA TTCACCTGTA CGCAGAGGCT GCTGTGCCCC AGGCTGCAGA GCCCATAGCC
ACCTTCTATG GGTTAAGGCA ACAGGCTGAG AAGGACTCTG CCAGCACGGA GCCATACTAC
TGCCTCTCAG ACTTCATCGC TCCCTTGCAT TCTGGCATCC GTGACTACCT GGGCCTGTTT
GCCGTTGCCT GCTTTGGGGT AGAAGAGCTG AGCAAGGCCT ATGAGGATGA TGGTGACGAC
TACAGCAGCA TCATGGTCAA GGCGCTGGGG GACCGGCTGG CAGAGAGCCC CTCGTTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu35028
Clone ID Related Accession (Same CDS sequence) XM_006711770
Accession Version XM_006711770.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2862bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product methionine synthase isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167186.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
ATGGATGGCT TGGTCAATAT AGTTGGAGGA TGCTGTGGGT CAACACCAGA TCATATCAGG 
GAAATTGCTG AAGCTGTGAA AAATTGTAAG CCTAGAGTTC CACCTGCCAC TGCTTTTGAA
GGACATATGT TACTGTCTGG TCTAGAGCCC TTCAGGATTG GACCGTACAC CAACTTTGTT
AACATTGGAG AGCGCTGTAA TGTTGCAGGA TCAAGGAAGT TTGCTAAACT CATCATGGCA
GGAAACTATG AAGAAGCCTT GTGTGTTGCC AAAGTGCAGG TGGAAATGGG AGCCCAGGTG
TTGGATGTCA ACATGGATGA TGGCATGCTA GATGGTCCAA GTGCAATGAC CAGATTTTGC
AACTTAATTG CTTCCGAGCC AGACATCGCA AAGGTACCTT TGTGCATCGA CTCCTCCAAT
TTTGCTGTGA TTGAAGCTGG GTTAAAGTGC TGCCAAGGGA AGTGCATTGT CAATAGCATT
AGTCTGAAGG AAGGAGAGGA CGACTTCTTG GAGAAGGCCA GGAAGATTAA AAAGTATGGA
GCTGCTATGG TGGTCATGGC TTTTGATGAA GAAGGACAGG CAACAGAAAC AGACACAAAA
ATCAGAGTGT GCACCCGGGC CTACCATCTG CTTGTGAAAA AACTGGGCTT TAATCCAAAT
GACATTATTT TTGACCCTAA TATCCTAACC ATTGGGACTG GAATGGAGGA ACACAACTTG
TATGCCATTA ATTTTATCCA TGCAACAAAA GTCATTAAAG AAACATTACC TGGAGCCAGA
ATAAGTGGAG GTCTTTCCAA CTTGTCCTTC TCCTTCCGAG GAATGGAAGC CATTCGAGAA
GCAATGCATG GGGTTTTCCT TTACCATGCA ATCAAGTCTG GCATGGACAT GGGGATAGTG
AATGCTGGAA ACCTCCCTGT GTATGATGAT ATCCATAAGG AACTTCTGCA GCTCTGTGAA
GATCTCATCT GGAATAAAGA CCCTGAGGCC ACTGAGAAGC TCTTACGTTA TGCCCAGACT
CAAGGCACAG GAGGGAAGAA AGTCATTCAG ACTGATGAGT GGAGAAATGG CCCTGTCGAA
GAACGCCTTG AGTATGCCCT TGTGAAGGGC ATTGAAAAAC ATATTATTGA GGATACTGAG
GAAGCCAGGT TAAACCAAAA AAAATATCCC CGACCTCTCA ATATAATTGA AGGACCCCTG
ATGAATGGAA TGAAAATTGT TGGTGATCTT TTTGGAGCTG GAAAAATGTT TCTACCTCAG
GTTATAAAGT CAGCCCGGGT TATGAAGAAG GCTGTTGGCC ACCTTATCCC TTTCATGGAA
AAAGAAAGAG AAGAAACCAG AGTGCTTAAC GGCACAGTAG AAGAAGAGGA CCCTTACCAG
GGCACCATCG TGCTGGCCAC TGTTAAAGGC GACGTGCACG ACATAGGCAA GAACATAGTT
GGAGTAGTCC TTGGCTGCAA TAATTTCCGA GTTATTGATT TAGGAGTCAT GACTCCATGT
GATAAGATAC TGAAAGCTGC TCTTGACCAC AAAGCAGATA TAATTGGCCT GTCAGGACTC
ATCACTCCTT CCCTGGATGA AATGATTTTT GTTGCCAAGG AAATGGAGAG ATTAGCTATA
AGGATTCCAT TGTTGATTGG AGGAGCAACC ACTTCAAAAA CCCACACAGC AGTTAAAATA
GCTCCGAGAT ACAGTGCACC TGTAATCCAT GTCCTGGACG CGTCCAAGAG TGTGGTGGTG
TGTTCCCAGC TGTTAGATGA AAATCTAAAG GATGAATACT TTGAGGAAAT CATGGAAGAA
TATGAAGATA TTAGACAGGA CCATTATGAG TCTCTCAAGG AGAGGAGATA CTTACCCTTA
AGTCAAGCCA GAAAAAGTGG TTTCCAAATG GATTGGCTGT CTGAACCTCA CCCAGTGAAG
CCCACGTTTA TTGGGACCCA GGTCTTTGAA GACTATGACC TGCAGAAGCT GGTGGACTAC
ATTGACTGGA AGCCTTTCTT TGATGTCTGG CAGCTCCGGG GCAAGTACCC GAATCGAGGC
TTTCCCAAGA TATTTAACGA CAAAACAGTA GGTGGAGAGG CCAGGAAGGT CTACGATGAT
GCCCACAATA TGCTGAACAC ACTGATTAGT CAAAAGAAAC TCCGGGCCCG GGGTGTGGTT
GGGTTCTGGC CAGCACAGAG TATCCAAGAC GACATTCACC TGTACGCAGA GGCTGCTGTG
CCCCAGGCTG CAGAGCCCAT AGCCACCTTC TATGGGTTAA GGCAACAGGC TGAGAAGGAC
TCTGCCAGCA CGGAGCCATA CTACTGCCTC TCAGACTTCA TCGCTCCCTT GCATTCTGGC
ATCCGTGACT ACCTGGGCCT GTTTGCCGTT GCCTGCTTTG GGGTAGAAGA GCTGAGCAAG
GCCTATGAGG ATGATGGTGA CGACTACAGC AGCATCATGG TCAAGGCGCT GGGGGACCGG
CTGGCAGAGG CCTTTGCAGA AGAGCTCCAT GAAAGAGTTC GCCGAGAACT GTGGGCCTAC
TGTGGCAGTG AGCAGCTGGA CGTCGCAGAC CTGCGCAGGC TGCGGTACAA GGGCATCCGC
CCGGCTCCTG GCTACCCCAG CCAGCCCGAC CACACCGAGA AGCTCACCAT GTGGAGACTC
GCAGACATCG AGCAGTCTAC AGGCATTAGG TTAACAGAAT CATTAGCAAT GGCACCTGCT
TCAGCAGTCT CAGGCCTCTA CTTCTCCAAT TTGAAGTCCA AATATTTTGC TGTGGGGAAG
ATTTCCAAGG ATCAGGTTGA GGATTATGCA TTGAGGAAGA ACATATCTGT GGCTGAGGTT
GAGAAATGGC TTGGACCCAT TTTGGGATAT GATACAGACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006711833.1
CDS308..3169
Misc Feature(1)311..391(+)
Misc Feature(2)395..3061(+)
Misc Feature(3)482..1252(+)
Misc Feature(4)506..1210(+)
Misc Feature(5)1382..2062(+)
Misc Feature(6)1496..2038(+)
Misc Feature(7)1724..1726(+)
Misc Feature(8)2261..3112(+)
Translation

Target ORF information:

RefSeq Version XM_006711770
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006711770

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
ATGGATGGCT TGGTCAATAT AGTTGGAGGA TGCTGTGGGT CAACACCAGA TCATATCAGG 
GAAATTGCTG AAGCTGTGAA AAATTGTAAG CCTAGAGTTC CACCTGCCAC TGCTTTTGAA
GGACATATGT TACTGTCTGG TCTAGAGCCC TTCAGGATTG GACCGTACAC CAACTTTGTT
AACATTGGAG AGCGCTGTAA TGTTGCAGGA TCAAGGAAGT TTGCTAAACT CATCATGGCA
GGAAACTATG AAGAAGCCTT GTGTGTTGCC AAAGTGCAGG TGGAAATGGG AGCCCAGGTG
TTGGATGTCA ACATGGATGA TGGCATGCTA GATGGTCCAA GTGCAATGAC CAGATTTTGC
AACTTAATTG CTTCCGAGCC AGACATCGCA AAGGTACCTT TGTGCATCGA CTCCTCCAAT
TTTGCTGTGA TTGAAGCTGG GTTAAAGTGC TGCCAAGGGA AGTGCATTGT CAATAGCATT
AGTCTGAAGG AAGGAGAGGA CGACTTCTTG GAGAAGGCCA GGAAGATTAA AAAGTATGGA
GCTGCTATGG TGGTCATGGC TTTTGATGAA GAAGGACAGG CAACAGAAAC AGACACAAAA
ATCAGAGTGT GCACCCGGGC CTACCATCTG CTTGTGAAAA AACTGGGCTT TAATCCAAAT
GACATTATTT TTGACCCTAA TATCCTAACC ATTGGGACTG GAATGGAGGA ACACAACTTG
TATGCCATTA ATTTTATCCA TGCAACAAAA GTCATTAAAG AAACATTACC TGGAGCCAGA
ATAAGTGGAG GTCTTTCCAA CTTGTCCTTC TCCTTCCGAG GAATGGAAGC CATTCGAGAA
GCAATGCATG GGGTTTTCCT TTACCATGCA ATCAAGTCTG GCATGGACAT GGGGATAGTG
AATGCTGGAA ACCTCCCTGT GTATGATGAT ATCCATAAGG AACTTCTGCA GCTCTGTGAA
GATCTCATCT GGAATAAAGA CCCTGAGGCC ACTGAGAAGC TCTTACGTTA TGCCCAGACT
CAAGGCACAG GAGGGAAGAA AGTCATTCAG ACTGATGAGT GGAGAAATGG CCCTGTCGAA
GAACGCCTTG AGTATGCCCT TGTGAAGGGC ATTGAAAAAC ATATTATTGA GGATACTGAG
GAAGCCAGGT TAAACCAAAA AAAATATCCC CGACCTCTCA ATATAATTGA AGGACCCCTG
ATGAATGGAA TGAAAATTGT TGGTGATCTT TTTGGAGCTG GAAAAATGTT TCTACCTCAG
GTTATAAAGT CAGCCCGGGT TATGAAGAAG GCTGTTGGCC ACCTTATCCC TTTCATGGAA
AAAGAAAGAG AAGAAACCAG AGTGCTTAAC GGCACAGTAG AAGAAGAGGA CCCTTACCAG
GGCACCATCG TGCTGGCCAC TGTTAAAGGC GACGTGCACG ACATAGGCAA GAACATAGTT
GGAGTAGTCC TTGGCTGCAA TAATTTCCGA GTTATTGATT TAGGAGTCAT GACTCCATGT
GATAAGATAC TGAAAGCTGC TCTTGACCAC AAAGCAGATA TAATTGGCCT GTCAGGACTC
ATCACTCCTT CCCTGGATGA AATGATTTTT GTTGCCAAGG AAATGGAGAG ATTAGCTATA
AGGATTCCAT TGTTGATTGG AGGAGCAACC ACTTCAAAAA CCCACACAGC AGTTAAAATA
GCTCCGAGAT ACAGTGCACC TGTAATCCAT GTCCTGGACG CGTCCAAGAG TGTGGTGGTG
TGTTCCCAGC TGTTAGATGA AAATCTAAAG GATGAATACT TTGAGGAAAT CATGGAAGAA
TATGAAGATA TTAGACAGGA CCATTATGAG TCTCTCAAGG AGAGGAGATA CTTACCCTTA
AGTCAAGCCA GAAAAAGTGG TTTCCAAATG GATTGGCTGT CTGAACCTCA CCCAGTGAAG
CCCACGTTTA TTGGGACCCA GGTCTTTGAA GACTATGACC TGCAGAAGCT GGTGGACTAC
ATTGACTGGA AGCCTTTCTT TGATGTCTGG CAGCTCCGGG GCAAGTACCC GAATCGAGGC
TTTCCCAAGA TATTTAACGA CAAAACAGTA GGTGGAGAGG CCAGGAAGGT CTACGATGAT
GCCCACAATA TGCTGAACAC ACTGATTAGT CAAAAGAAAC TCCGGGCCCG GGGTGTGGTT
GGGTTCTGGC CAGCACAGAG TATCCAAGAC GACATTCACC TGTACGCAGA GGCTGCTGTG
CCCCAGGCTG CAGAGCCCAT AGCCACCTTC TATGGGTTAA GGCAACAGGC TGAGAAGGAC
TCTGCCAGCA CGGAGCCATA CTACTGCCTC TCAGACTTCA TCGCTCCCTT GCATTCTGGC
ATCCGTGACT ACCTGGGCCT GTTTGCCGTT GCCTGCTTTG GGGTAGAAGA GCTGAGCAAG
GCCTATGAGG ATGATGGTGA CGACTACAGC AGCATCATGG TCAAGGCGCT GGGGGACCGG
CTGGCAGAGG CCTTTGCAGA AGAGCTCCAT GAAAGAGTTC GCCGAGAACT GTGGGCCTAC
TGTGGCAGTG AGCAGCTGGA CGTCGCAGAC CTGCGCAGGC TGCGGTACAA GGGCATCCGC
CCGGCTCCTG GCTACCCCAG CCAGCCCGAC CACACCGAGA AGCTCACCAT GTGGAGACTC
GCAGACATCG AGCAGTCTAC AGGCATTAGG TTAACAGAAT CATTAGCAAT GGCACCTGCT
TCAGCAGTCT CAGGCCTCTA CTTCTCCAAT TTGAAGTCCA AATATTTTGC TGTGGGGAAG
ATTTCCAAGG ATCAGGTTGA GGATTATGCA TTGAGGAAGA ACATATCTGT GGCTGAGGTT
GAGAAATGGC TTGGACCCAT TTTGGGATAT GATACAGACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59095
Clone ID Related Accession (Same CDS sequence) XM_011544194
Accession Version XM_011544194.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product methionine synthase isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167186.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGTCTGCGG GGCTCAGAGC CGGATGTCAC GTCGTCCTCC TCTGCCGGTT TTCTCTTGGG 
TCCTTTTCCG TGCCGTCCCG CGACTCCGCC TCTGGCCGCG CGTGTCTGGC TGCTAGGCCG
ACACCAAGGA CTGGCCGGGT ACCCGGGAAG AAAGCACGTG CTCCAGCAGT TGCCGCGCCC
AGCCCCGAGA GAGGCCCTAG GGCGCTGCGG GCTTTCGGGG TCCGCAGTCC CCCCGCGACG
CGAGCCAACG GGAGGCGTCA AAAGACCCGG GCCTTGTGTG GCAGGCTCGC CTGGCGCTGG
CTGGCGTGGC CCTTGGCCGT CGTCACCTGT GGAGAGCACG TCTTCTCTGC CGCGCCCTCT
GCGCAAGGAG GAGACTCGAC AACATGTCAC CCGCGCTCCA AGACCTGTCG CAACCCGGAA
TACTTGCTGG CTGGGGCAGA TATCATTGAA ACAAATACTT TTAGCAGCAC TAGTATTGCC
CAAGCTGACT ATGGCCTTGA ACACTTGGCC TACCGGATGA ACATGTGCTC TGCAGGAGTG
GCCAGAAAAG CTGCCGAGGA GGTAACTCTC CAGACAGGAA TTAAGAGGTT TGTGGCAGGG
GCTCTGGGTC CGACTAATAA GACACTCTCT GTGTCCCCAT CTGTGGAAAG GCCGGATTAT
AGGAACATCA CATTTGATGA GCTTGTTGAA GCATACCAAG AGCAGGCCAA AGGACTTCTG
GATGGCGGGG TTGATATCTT ACTCATTGAA ACTATTTTTG ATACTGCCAA TGCCAAGGCA
GCCTTGTTTG CACTCCAAAA TCTTTTTGAG GAGAAATATG CTCCCCGGCC TATCTTTATT
TCAGGGACGA TCGTTGATAA AAGTGGGCGG ACTCTTTCCG GACAGACAGG AGAGGGATTT
GTCATCAGCG TGTCTCATGG AGAACCACTC TGCATTGGAT TAAATTGTGC TTTGGGTGCA
GCTGAAATGA GACCTTTTAT TGAAATAATT GGAAAATGTA CAACAGCCTA TGTCCTCTGT
TATCCCAATG CAGGTCTTCC CAACACCTTT GGTGACTATG ATGAAACGCC TTCTATGATG
GCCAAGCACC TAAAGGATTT TGCTATGGAT GGCTTGGTCA ATATAGTTGG AGGATGCTGT
GGGTCAACAC CAGATCATAT CAGGGAAATT GCTGAAGCTG TGAAAAATTG TAAGCCTAGA
GTTCCACCTG CCACTGCTTT TGAAGGACAT ATGTTACTGT CTGGTCTAGA GCCCTTCAGG
ATTGGACCGT ACACCAACTT TGTTAACATT GGAGAGCGCT GTAATGTTGC AGGATCAAGG
AAGTTTGCTA AACTCATCAT GGCAGGAAAC TATGAAGAAG CCTTGTGTGT TGCCAAAGTG
CAGGTGGAAA TGGGAGCCCA GGTGTTGGAT GTCAACATGG ATGATGGCAT GCTAGATGGT
CCAAGTGCAA TGACCAGATT TTGCAACTTA ATTGCTTCCG AGCCAGACAT CGCAAAGGTA
CCTTTGTGCA TCGACTCCTC CAATTTTGCT GTGATTGAAG CTGGGTTAAA GTGCTGCCAA
GGGAAGTGCA TTGTCAATAG CATTAGTCTG AAGGAAGGAG AGGACGACTT CTTGGAGAAG
GCCAGGAAGA TTAAAAAGTA TGGAGCTGCT ATGGTGGTCA TGGCTTTTGA TGAAGAAGGA
CAGGCAACAG AAACAGACAC AAAAATCAGA GTGTGCACCC GGGCCTACCA TCTGCTTGTG
AAAAAACTGG GCTTTAATCC AAATGACATT ATTTTTGACC CTAATATCCT AACCATTGGG
ACTGGAATGG AGGAACACAA CTTGTATGCC ATTAATTTTA TCCATGCAAC AAAAGTCATT
AAAGAAACAT TACCTGGAGC CAGAATAAGT GGAGGTCTTT CCAACTTGTC CTTCTCCTTC
CGAGGAATGG AAGCCATTCG AGAAGCAATG CATGGGGTTT TCCTTTACCA TGCAATCAAG
TCTGGCATGG ACATGGGGAT AGTGAATGCT GGAAACCTCC CTGTGTATGA TGATATCCAT
AAGGAACTTC TGCAGCTCTG TGAAGATCTC ATCTGGAATA AAGACCCTGA GGCCACTGAG
AAGCTCTTAC GTTATGCCCA GACTCAAGGC ACAGGAGGGA AGAAAGTCAT TCAGACTGAT
GAGTGGAGAA ATGGCCCTGT CGAAGAACGC CTTGAGTATG CCCTTGTGAA GGGCATTGAA
AAACATATTA TTGAGGATAC TGAGGAAGCC AGGTTAAACC AAAAAAAATA TCCCCGACCT
CTCAATATAA TTGAAGGACC CCTGATGAAT GGAATGAAAA TTGTTGGTGA TCTTTTTGGA
GCTGGAAAAA TGTTTCTACC TCAGGTTATA AAGTCAGCCC GGGTTATGAA GAAGGCTGTT
GGCCACCTTA TCCCTTTCAT GGAAAAAGAA AGAGAAGAAA CCAGAGTGCT TAACGGCACA
GTAGAAGAAG AGGACCCTTA CCAGGGCACC ATCGTGCTGG CCACTGTTAA AGGCGACGTG
CACGACATAG GCAAGAACAT AGTTGGAGTA GTCCTTGGCT GCAATAATTT CCGAGTTATT
GATTTAGGAG TCATGACTCC ATGTGATAAG ATACTGAAAG CTGCTCTTGA CCACAAAGCA
GATATAATTG GCCTGTCAGG ACTCATCACT CCTTCCCTGG ATGAAATGAT TTTTGTTGCC
AAGGAAATGG AGAGATTAGC TATAAGGATT CCATTGTTGA TTGGAGGAGC AACCACTTCA
AAAACCCACA CAGCAGTTAA AATAGCTCCG AGATACAGTG CACCTGTAAT CCATGTCCTG
GACGCGTCCA AGAGTGTGGT GGTGTGTTCC CAGCTGTTAG ATGAAAATCT AAAGGATGAA
TACTTTGAGG AAATCATGGA AGAATATGAA GATATTAGAC AGGACCATTA TGAGTCTCTC
AAGGAGAGGA GATACTTACC CTTAAGTCAA GCCAGAAAAA GTGGTTTCCA AATGGATTGG
CTGTCTGAAC CTCACCCAGT GAAGCCCACG TTTATTGGGA CCCAGGTCTT TGAAGACTAT
GACCTGCAGA AGCTGGTGGA CTACATTGAC TGGAAGCCTT TCTTTGATGT CTGGCAGCTC
CGGGGCAAGT ACCCGAATCG AGGCTTTCCC AAGATATTTA ACGACAAAAC AGTAGGTGGA
GAGGCCAGGA AGGTCTACGA TGATGCCCAC AATATGCTGA ACACACTGAT TAGTCAAAAG
AAACTCCGGG CCCGGGGTGT GGTTGGGTTC TGGCCAGCAC AGAGTATCCA AGACGACATT
CACCTGTACG CAGAGGCTGC TGTGCCCCAG GCTGCAGAGC CCATAGCCAC CTTCTATGGG
TTAAGGCAAC AGGCTGAGAA GGACTCTGCC AGCACGGAGC CATACTACTG CCTCTCAGAC
TTCATCGCTC CCTTGCATTC TGGCATCCGT GACTACCTGG GCCTGTTTGC CGTTGCCTGC
TTTGGGGTAG AAGAGCTGAG CAAGGCCTAT GAGGATGATG GTGACGACTA CAGCAGCATC
ATGGTCAAGG CGCTGGGGGA CCGGCTGGCA GAGGCCTTTG CAGAAGAGCT CCATGAAAGA
GTTCGCCGAG AACTGTGGGC CTACTGTGGC AGTGAGCAGC TGGACGTCGC AGACCTGCGC
AGGCTGCGGT ACAAGGGCAT CCGCCCGGCT CCTGGCTACC CCAGCCAGCC CGACCACACC
GAGAAGCTCA CCATGTGGAG ACTCGCAGAC ATCGAGCAGT CTACAGGCAT TAGGTTAACA
GAATCATTAG CAATGGCACC TGCTTCAGCA GTCTCAGGCC TCTACTTCTC CAATTTGAAG
TCCAAATATT TTGCTGTGGG GAAGATTTCC AAGGATCAGG TTGAGGATTA TGCATTGAGG
AAGAACATAT CTGTGGCTGA GGTTGAGAAA TGGCTTGGAC CCATTTTGGG ATATGATACA
GACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542496.1
CDS1018..4983
Misc Feature(1)1435..4968(+)
Misc Feature(2)1435..2205(+)
Misc Feature(3)2296..3066(+)
Misc Feature(4)2320..3024(+)
Misc Feature(5)3196..3876(+)
Misc Feature(6)3310..3852(+)
Misc Feature(7)3538..3540(+)
Misc Feature(8)4075..4926(+)
Translation

Target ORF information:

RefSeq Version XM_011544194
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011544194

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGTCTGCGG GGCTCAGAGC CGGATGTCAC GTCGTCCTCC TCTGCCGGTT TTCTCTTGGG 
TCCTTTTCCG TGCCGTCCCG CGACTCCGCC TCTGGCCGCG CGTGTCTGGC TGCTAGGCCG
ACACCAAGGA CTGGCCGGGT ACCCGGGAAG AAAGCACGTG CTCCAGCAGT TGCCGCGCCC
AGCCCCGAGA GAGGCCCTAG GGCGCTGCGG GCTTTCGGGG TCCGCAGTCC CCCCGCGACG
CGAGCCAACG GGAGGCGTCA AAAGACCCGG GCCTTGTGTG GCAGGCTCGC CTGGCGCTGG
CTGGCGTGGC CCTTGGCCGT CGTCACCTGT GGAGAGCACG TCTTCTCTGC CGCGCCCTCT
GCGCAAGGAG GAGACTCGAC AACATGTCAC CCGCGCTCCA AGACCTGTCG CAACCCGGAA
TACTTGCTGG CTGGGGCAGA TATCATTGAA ACAAATACTT TTAGCAGCAC TAGTATTGCC
CAAGCTGACT ATGGCCTTGA ACACTTGGCC TACCGGATGA ACATGTGCTC TGCAGGAGTG
GCCAGAAAAG CTGCCGAGGA GGTAACTCTC CAGACAGGAA TTAAGAGGTT TGTGGCAGGG
GCTCTGGGTC CGACTAATAA GACACTCTCT GTGTCCCCAT CTGTGGAAAG GCCGGATTAT
AGGAACATCA CATTTGATGA GCTTGTTGAA GCATACCAAG AGCAGGCCAA AGGACTTCTG
GATGGCGGGG TTGATATCTT ACTCATTGAA ACTATTTTTG ATACTGCCAA TGCCAAGGCA
GCCTTGTTTG CACTCCAAAA TCTTTTTGAG GAGAAATATG CTCCCCGGCC TATCTTTATT
TCAGGGACGA TCGTTGATAA AAGTGGGCGG ACTCTTTCCG GACAGACAGG AGAGGGATTT
GTCATCAGCG TGTCTCATGG AGAACCACTC TGCATTGGAT TAAATTGTGC TTTGGGTGCA
GCTGAAATGA GACCTTTTAT TGAAATAATT GGAAAATGTA CAACAGCCTA TGTCCTCTGT
TATCCCAATG CAGGTCTTCC CAACACCTTT GGTGACTATG ATGAAACGCC TTCTATGATG
GCCAAGCACC TAAAGGATTT TGCTATGGAT GGCTTGGTCA ATATAGTTGG AGGATGCTGT
GGGTCAACAC CAGATCATAT CAGGGAAATT GCTGAAGCTG TGAAAAATTG TAAGCCTAGA
GTTCCACCTG CCACTGCTTT TGAAGGACAT ATGTTACTGT CTGGTCTAGA GCCCTTCAGG
ATTGGACCGT ACACCAACTT TGTTAACATT GGAGAGCGCT GTAATGTTGC AGGATCAAGG
AAGTTTGCTA AACTCATCAT GGCAGGAAAC TATGAAGAAG CCTTGTGTGT TGCCAAAGTG
CAGGTGGAAA TGGGAGCCCA GGTGTTGGAT GTCAACATGG ATGATGGCAT GCTAGATGGT
CCAAGTGCAA TGACCAGATT TTGCAACTTA ATTGCTTCCG AGCCAGACAT CGCAAAGGTA
CCTTTGTGCA TCGACTCCTC CAATTTTGCT GTGATTGAAG CTGGGTTAAA GTGCTGCCAA
GGGAAGTGCA TTGTCAATAG CATTAGTCTG AAGGAAGGAG AGGACGACTT CTTGGAGAAG
GCCAGGAAGA TTAAAAAGTA TGGAGCTGCT ATGGTGGTCA TGGCTTTTGA TGAAGAAGGA
CAGGCAACAG AAACAGACAC AAAAATCAGA GTGTGCACCC GGGCCTACCA TCTGCTTGTG
AAAAAACTGG GCTTTAATCC AAATGACATT ATTTTTGACC CTAATATCCT AACCATTGGG
ACTGGAATGG AGGAACACAA CTTGTATGCC ATTAATTTTA TCCATGCAAC AAAAGTCATT
AAAGAAACAT TACCTGGAGC CAGAATAAGT GGAGGTCTTT CCAACTTGTC CTTCTCCTTC
CGAGGAATGG AAGCCATTCG AGAAGCAATG CATGGGGTTT TCCTTTACCA TGCAATCAAG
TCTGGCATGG ACATGGGGAT AGTGAATGCT GGAAACCTCC CTGTGTATGA TGATATCCAT
AAGGAACTTC TGCAGCTCTG TGAAGATCTC ATCTGGAATA AAGACCCTGA GGCCACTGAG
AAGCTCTTAC GTTATGCCCA GACTCAAGGC ACAGGAGGGA AGAAAGTCAT TCAGACTGAT
GAGTGGAGAA ATGGCCCTGT CGAAGAACGC CTTGAGTATG CCCTTGTGAA GGGCATTGAA
AAACATATTA TTGAGGATAC TGAGGAAGCC AGGTTAAACC AAAAAAAATA TCCCCGACCT
CTCAATATAA TTGAAGGACC CCTGATGAAT GGAATGAAAA TTGTTGGTGA TCTTTTTGGA
GCTGGAAAAA TGTTTCTACC TCAGGTTATA AAGTCAGCCC GGGTTATGAA GAAGGCTGTT
GGCCACCTTA TCCCTTTCAT GGAAAAAGAA AGAGAAGAAA CCAGAGTGCT TAACGGCACA
GTAGAAGAAG AGGACCCTTA CCAGGGCACC ATCGTGCTGG CCACTGTTAA AGGCGACGTG
CACGACATAG GCAAGAACAT AGTTGGAGTA GTCCTTGGCT GCAATAATTT CCGAGTTATT
GATTTAGGAG TCATGACTCC ATGTGATAAG ATACTGAAAG CTGCTCTTGA CCACAAAGCA
GATATAATTG GCCTGTCAGG ACTCATCACT CCTTCCCTGG ATGAAATGAT TTTTGTTGCC
AAGGAAATGG AGAGATTAGC TATAAGGATT CCATTGTTGA TTGGAGGAGC AACCACTTCA
AAAACCCACA CAGCAGTTAA AATAGCTCCG AGATACAGTG CACCTGTAAT CCATGTCCTG
GACGCGTCCA AGAGTGTGGT GGTGTGTTCC CAGCTGTTAG ATGAAAATCT AAAGGATGAA
TACTTTGAGG AAATCATGGA AGAATATGAA GATATTAGAC AGGACCATTA TGAGTCTCTC
AAGGAGAGGA GATACTTACC CTTAAGTCAA GCCAGAAAAA GTGGTTTCCA AATGGATTGG
CTGTCTGAAC CTCACCCAGT GAAGCCCACG TTTATTGGGA CCCAGGTCTT TGAAGACTAT
GACCTGCAGA AGCTGGTGGA CTACATTGAC TGGAAGCCTT TCTTTGATGT CTGGCAGCTC
CGGGGCAAGT ACCCGAATCG AGGCTTTCCC AAGATATTTA ACGACAAAAC AGTAGGTGGA
GAGGCCAGGA AGGTCTACGA TGATGCCCAC AATATGCTGA ACACACTGAT TAGTCAAAAG
AAACTCCGGG CCCGGGGTGT GGTTGGGTTC TGGCCAGCAC AGAGTATCCA AGACGACATT
CACCTGTACG CAGAGGCTGC TGTGCCCCAG GCTGCAGAGC CCATAGCCAC CTTCTATGGG
TTAAGGCAAC AGGCTGAGAA GGACTCTGCC AGCACGGAGC CATACTACTG CCTCTCAGAC
TTCATCGCTC CCTTGCATTC TGGCATCCGT GACTACCTGG GCCTGTTTGC CGTTGCCTGC
TTTGGGGTAG AAGAGCTGAG CAAGGCCTAT GAGGATGATG GTGACGACTA CAGCAGCATC
ATGGTCAAGG CGCTGGGGGA CCGGCTGGCA GAGGCCTTTG CAGAAGAGCT CCATGAAAGA
GTTCGCCGAG AACTGTGGGC CTACTGTGGC AGTGAGCAGC TGGACGTCGC AGACCTGCGC
AGGCTGCGGT ACAAGGGCAT CCGCCCGGCT CCTGGCTACC CCAGCCAGCC CGACCACACC
GAGAAGCTCA CCATGTGGAG ACTCGCAGAC ATCGAGCAGT CTACAGGCAT TAGGTTAACA
GAATCATTAG CAATGGCACC TGCTTCAGCA GTCTCAGGCC TCTACTTCTC CAATTTGAAG
TCCAAATATT TTGCTGTGGG GAAGATTTCC AAGGATCAGG TTGAGGATTA TGCATTGAGG
AAGAACATAT CTGTGGCTGA GGTTGAGAAA TGGCTTGGAC CCATTTTGGG ATATGATACA
GACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24754
Clone ID Related Accession (Same CDS sequence) NM_000254
Accession Version NM_000254.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product methionine synthase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC377743.1, U73338.1, U75743.1 and AL359259.18. This sequence is a reference standard in the RefSeqGene project. On Mar 12, 2008 this sequence version replaced gi:4557764. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## regulatory uORF :: PMID: 17683808 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: U73338.1, U75743.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGTCACCCG CGCTCCAAGA CCTGTCGCAA CCCGAAGGTC TGAAGAAAAC CCTGCGGGAT 
GAGATCAATG CCATTCTGCA GAAGAGGATT ATGGTGCTGG ATGGAGGGAT GGGGACCATG
ATCCAGCGGG AGAAGCTAAA CGAAGAACAC TTCCGAGGTC AGGAATTTAA AGATCATGCC
AGGCCGCTGA AAGGCAACAA TGACATTTTA AGTATAACTC AGCCTGATGT CATTTACCAA
ATCCATAAGG AATACTTGCT GGCTGGGGCA GATATCATTG AAACAAATAC TTTTAGCAGC
ACTAGTATTG CCCAAGCTGA CTATGGCCTT GAACACTTGG CCTACCGGAT GAACATGTGC
TCTGCAGGAG TGGCCAGAAA AGCTGCCGAG GAGGTAACTC TCCAGACAGG AATTAAGAGG
TTTGTGGCAG GGGCTCTGGG TCCGACTAAT AAGACACTCT CTGTGTCCCC ATCTGTGGAA
AGGCCGGATT ATAGGAACAT CACATTTGAT GAGCTTGTTG AAGCATACCA AGAGCAGGCC
AAAGGACTTC TGGATGGCGG GGTTGATATC TTACTCATTG AAACTATTTT TGATACTGCC
AATGCCAAGG CAGCCTTGTT TGCACTCCAA AATCTTTTTG AGGAGAAATA TGCTCCCCGG
CCTATCTTTA TTTCAGGGAC GATCGTTGAT AAAAGTGGGC GGACTCTTTC CGGACAGACA
GGAGAGGGAT TTGTCATCAG CGTGTCTCAT GGAGAACCAC TCTGCATTGG ATTAAATTGT
GCTTTGGGTG CAGCTGAAAT GAGACCTTTT ATTGAAATAA TTGGAAAATG TACAACAGCC
TATGTCCTCT GTTATCCCAA TGCAGGTCTT CCCAACACCT TTGGTGACTA TGATGAAACG
CCTTCTATGA TGGCCAAGCA CCTAAAGGAT TTTGCTATGG ATGGCTTGGT CAATATAGTT
GGAGGATGCT GTGGGTCAAC ACCAGATCAT ATCAGGGAAA TTGCTGAAGC TGTGAAAAAT
TGTAAGCCTA GAGTTCCACC TGCCACTGCT TTTGAAGGAC ATATGTTACT GTCTGGTCTA
GAGCCCTTCA GGATTGGACC GTACACCAAC TTTGTTAACA TTGGAGAGCG CTGTAATGTT
GCAGGATCAA GGAAGTTTGC TAAACTCATC ATGGCAGGAA ACTATGAAGA AGCCTTGTGT
GTTGCCAAAG TGCAGGTGGA AATGGGAGCC CAGGTGTTGG ATGTCAACAT GGATGATGGC
ATGCTAGATG GTCCAAGTGC AATGACCAGA TTTTGCAACT TAATTGCTTC CGAGCCAGAC
ATCGCAAAGG TACCTTTGTG CATCGACTCC TCCAATTTTG CTGTGATTGA AGCTGGGTTA
AAGTGCTGCC AAGGGAAGTG CATTGTCAAT AGCATTAGTC TGAAGGAAGG AGAGGACGAC
TTCTTGGAGA AGGCCAGGAA GATTAAAAAG TATGGAGCTG CTATGGTGGT CATGGCTTTT
GATGAAGAAG GACAGGCAAC AGAAACAGAC ACAAAAATCA GAGTGTGCAC CCGGGCCTAC
CATCTGCTTG TGAAAAAACT GGGCTTTAAT CCAAATGACA TTATTTTTGA CCCTAATATC
CTAACCATTG GGACTGGAAT GGAGGAACAC AACTTGTATG CCATTAATTT TATCCATGCA
ACAAAAGTCA TTAAAGAAAC ATTACCTGGA GCCAGAATAA GTGGAGGTCT TTCCAACTTG
TCCTTCTCCT TCCGAGGAAT GGAAGCCATT CGAGAAGCAA TGCATGGGGT TTTCCTTTAC
CATGCAATCA AGTCTGGCAT GGACATGGGG ATAGTGAATG CTGGAAACCT CCCTGTGTAT
GATGATATCC ATAAGGAACT TCTGCAGCTC TGTGAAGATC TCATCTGGAA TAAAGACCCT
GAGGCCACTG AGAAGCTCTT ACGTTATGCC CAGACTCAAG GCACAGGAGG GAAGAAAGTC
ATTCAGACTG ATGAGTGGAG AAATGGCCCT GTCGAAGAAC GCCTTGAGTA TGCCCTTGTG
AAGGGCATTG AAAAACATAT TATTGAGGAT ACTGAGGAAG CCAGGTTAAA CCAAAAAAAA
TATCCCCGAC CTCTCAATAT AATTGAAGGA CCCCTGATGA ATGGAATGAA AATTGTTGGT
GATCTTTTTG GAGCTGGAAA AATGTTTCTA CCTCAGGTTA TAAAGTCAGC CCGGGTTATG
AAGAAGGCTG TTGGCCACCT TATCCCTTTC ATGGAAAAAG AAAGAGAAGA AACCAGAGTG
CTTAACGGCA CAGTAGAAGA AGAGGACCCT TACCAGGGCA CCATCGTGCT GGCCACTGTT
AAAGGCGACG TGCACGACAT AGGCAAGAAC ATAGTTGGAG TAGTCCTTGG CTGCAATAAT
TTCCGAGTTA TTGATTTAGG AGTCATGACT CCATGTGATA AGATACTGAA AGCTGCTCTT
GACCACAAAG CAGATATAAT TGGCCTGTCA GGACTCATCA CTCCTTCCCT GGATGAAATG
ATTTTTGTTG CCAAGGAAAT GGAGAGATTA GCTATAAGGA TTCCATTGTT GATTGGAGGA
GCAACCACTT CAAAAACCCA CACAGCAGTT AAAATAGCTC CGAGATACAG TGCACCTGTA
ATCCATGTCC TGGACGCGTC CAAGAGTGTG GTGGTGTGTT CCCAGCTGTT AGATGAAAAT
CTAAAGGATG AATACTTTGA GGAAATCATG GAAGAATATG AAGATATTAG ACAGGACCAT
TATGAGTCTC TCAAGGAGAG GAGATACTTA CCCTTAAGTC AAGCCAGAAA AAGTGGTTTC
CAAATGGATT GGCTGTCTGA ACCTCACCCA GTGAAGCCCA CGTTTATTGG GACCCAGGTC
TTTGAAGACT ATGACCTGCA GAAGCTGGTG GACTACATTG ACTGGAAGCC TTTCTTTGAT
GTCTGGCAGC TCCGGGGCAA GTACCCGAAT CGAGGCTTTC CCAAGATATT TAACGACAAA
ACAGTAGGTG GAGAGGCCAG GAAGGTCTAC GATGATGCCC ACAATATGCT GAACACACTG
ATTAGTCAAA AGAAACTCCG GGCCCGGGGT GTGGTTGGGT TCTGGCCAGC ACAGAGTATC
CAAGACGACA TTCACCTGTA CGCAGAGGCT GCTGTGCCCC AGGCTGCAGA GCCCATAGCC
ACCTTCTATG GGTTAAGGCA ACAGGCTGAG AAGGACTCTG CCAGCACGGA GCCATACTAC
TGCCTCTCAG ACTTCATCGC TCCCTTGCAT TCTGGCATCC GTGACTACCT GGGCCTGTTT
GCCGTTGCCT GCTTTGGGGT AGAAGAGCTG AGCAAGGCCT ATGAGGATGA TGGTGACGAC
TACAGCAGCA TCATGGTCAA GGCGCTGGGG GACCGGCTGG CAGAGGCCTT TGCAGAAGAG
CTCCATGAAA GAGTTCGCCG AGAACTGTGG GCCTACTGTG GCAGTGAGCA GCTGGACGTC
GCAGACCTGC GCAGGCTGCG GTACAAGGGC ATCCGCCCGG CTCCTGGCTA CCCCAGCCAG
CCCGACCACA CCGAGAAGCT CACCATGTGG AGACTCGCAG ACATCGAGCA GTCTACAGGC
ATTAGGTTAA CAGAATCATT AGCAATGGCA CCTGCTTCAG CAGTCTCAGG CCTCTACTTC
TCCAATTTGA AGTCCAAATA TTTTGCTGTG GGGAAGATTT CCAAGGATCA GGTTGAGGAT
TATGCATTGA GGAAGAACAT ATCTGTGGCT GAGGTTGAGA AATGGCTTGG ACCCATTTTG
GGATATGATA CAGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000245.2
CDS424..4221
Misc Feature(1)41..466(+)
Misc Feature(2)64..156(+)
Misc Feature(3)238..240(+)
Misc Feature(4)478..4206(+)
Misc Feature(5)484..1443(+)
Misc Feature(6)1534..2304(+)
Misc Feature(7)1558..2262(+)
Misc Feature(8)2434..3114(+)
Misc Feature(9)2548..3090(+)
Misc Feature(10)2776..2778(+)
Misc Feature(11)3001..3006(+)
Misc Feature(12)3313..4164(+)
Misc Feature(13)4102..4107(+)
Exon (1)1..457
Gene:MTR
Gene Synonym:
Exon (2)458..672
Gene:MTR
Gene Synonym:
Exon (3)673..762
Gene:MTR
Gene Synonym:
Exon (4)763..832
Gene:MTR
Gene Synonym:
Exon (5)833..925
Gene:MTR
Gene Synonym:
Exon (6)926..1032
Gene:MTR
Gene Synonym:
Exon (7)1033..1092
Gene:MTR
Gene Synonym:
Exon (8)1093..1187
Gene:MTR
Gene Synonym:
Exon (9)1188..1288
Gene:MTR
Gene Synonym:
Exon (10)1289..1350
Gene:MTR
Gene Synonym:
Exon (11)1351..1418
Gene:MTR
Gene Synonym:
Exon (12)1419..1498
Gene:MTR
Gene Synonym:
Exon (13)1499..1611
Gene:MTR
Gene Synonym:
Exon (14)1612..1752
Gene:MTR
Gene Synonym:
Exon (15)1753..1938
Gene:MTR
Gene Synonym:
Exon (16)1939..2118
Gene:MTR
Gene Synonym:
Exon (17)2119..2235
Gene:MTR
Gene Synonym:
Exon (18)2236..2376
Gene:MTR
Gene Synonym:
Exon (19)2377..2466
Gene:MTR
Gene Synonym:
Exon (20)2467..2619
Gene:MTR
Gene Synonym:
Exon (21)2620..2727
Gene:MTR
Gene Synonym:
Exon (22)2728..2828
Gene:MTR
Gene Synonym:
Exon (23)2829..2896
Gene:MTR
Gene Synonym:
Exon (24)2897..3017
Gene:MTR
Gene Synonym:
Exon (25)3018..3099
Gene:MTR
Gene Synonym:
Exon (26)3100..3198
Gene:MTR
Gene Synonym:
Exon (27)3199..3274
Gene:MTR
Gene Synonym:
Exon (28)3275..3430
Gene:MTR
Gene Synonym:
Exon (29)3431..3627
Gene:MTR
Gene Synonym:
Exon (30)3628..3828
Gene:MTR
Gene Synonym:
Exon (31)3829..4021
Gene:MTR
Gene Synonym:
Exon (32)4022..4134
Gene:MTR
Gene Synonym:
Exon (33)4135..10558
Gene:MTR
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000254
Organism Homo sapiens (human)
Definition Homo sapiens 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000254

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGTCACCCG CGCTCCAAGA CCTGTCGCAA CCCGAAGGTC TGAAGAAAAC CCTGCGGGAT 
GAGATCAATG CCATTCTGCA GAAGAGGATT ATGGTGCTGG ATGGAGGGAT GGGGACCATG
ATCCAGCGGG AGAAGCTAAA CGAAGAACAC TTCCGAGGTC AGGAATTTAA AGATCATGCC
AGGCCGCTGA AAGGCAACAA TGACATTTTA AGTATAACTC AGCCTGATGT CATTTACCAA
ATCCATAAGG AATACTTGCT GGCTGGGGCA GATATCATTG AAACAAATAC TTTTAGCAGC
ACTAGTATTG CCCAAGCTGA CTATGGCCTT GAACACTTGG CCTACCGGAT GAACATGTGC
TCTGCAGGAG TGGCCAGAAA AGCTGCCGAG GAGGTAACTC TCCAGACAGG AATTAAGAGG
TTTGTGGCAG GGGCTCTGGG TCCGACTAAT AAGACACTCT CTGTGTCCCC ATCTGTGGAA
AGGCCGGATT ATAGGAACAT CACATTTGAT GAGCTTGTTG AAGCATACCA AGAGCAGGCC
AAAGGACTTC TGGATGGCGG GGTTGATATC TTACTCATTG AAACTATTTT TGATACTGCC
AATGCCAAGG CAGCCTTGTT TGCACTCCAA AATCTTTTTG AGGAGAAATA TGCTCCCCGG
CCTATCTTTA TTTCAGGGAC GATCGTTGAT AAAAGTGGGC GGACTCTTTC CGGACAGACA
GGAGAGGGAT TTGTCATCAG CGTGTCTCAT GGAGAACCAC TCTGCATTGG ATTAAATTGT
GCTTTGGGTG CAGCTGAAAT GAGACCTTTT ATTGAAATAA TTGGAAAATG TACAACAGCC
TATGTCCTCT GTTATCCCAA TGCAGGTCTT CCCAACACCT TTGGTGACTA TGATGAAACG
CCTTCTATGA TGGCCAAGCA CCTAAAGGAT TTTGCTATGG ATGGCTTGGT CAATATAGTT
GGAGGATGCT GTGGGTCAAC ACCAGATCAT ATCAGGGAAA TTGCTGAAGC TGTGAAAAAT
TGTAAGCCTA GAGTTCCACC TGCCACTGCT TTTGAAGGAC ATATGTTACT GTCTGGTCTA
GAGCCCTTCA GGATTGGACC GTACACCAAC TTTGTTAACA TTGGAGAGCG CTGTAATGTT
GCAGGATCAA GGAAGTTTGC TAAACTCATC ATGGCAGGAA ACTATGAAGA AGCCTTGTGT
GTTGCCAAAG TGCAGGTGGA AATGGGAGCC CAGGTGTTGG ATGTCAACAT GGATGATGGC
ATGCTAGATG GTCCAAGTGC AATGACCAGA TTTTGCAACT TAATTGCTTC CGAGCCAGAC
ATCGCAAAGG TACCTTTGTG CATCGACTCC TCCAATTTTG CTGTGATTGA AGCTGGGTTA
AAGTGCTGCC AAGGGAAGTG CATTGTCAAT AGCATTAGTC TGAAGGAAGG AGAGGACGAC
TTCTTGGAGA AGGCCAGGAA GATTAAAAAG TATGGAGCTG CTATGGTGGT CATGGCTTTT
GATGAAGAAG GACAGGCAAC AGAAACAGAC ACAAAAATCA GAGTGTGCAC CCGGGCCTAC
CATCTGCTTG TGAAAAAACT GGGCTTTAAT CCAAATGACA TTATTTTTGA CCCTAATATC
CTAACCATTG GGACTGGAAT GGAGGAACAC AACTTGTATG CCATTAATTT TATCCATGCA
ACAAAAGTCA TTAAAGAAAC ATTACCTGGA GCCAGAATAA GTGGAGGTCT TTCCAACTTG
TCCTTCTCCT TCCGAGGAAT GGAAGCCATT CGAGAAGCAA TGCATGGGGT TTTCCTTTAC
CATGCAATCA AGTCTGGCAT GGACATGGGG ATAGTGAATG CTGGAAACCT CCCTGTGTAT
GATGATATCC ATAAGGAACT TCTGCAGCTC TGTGAAGATC TCATCTGGAA TAAAGACCCT
GAGGCCACTG AGAAGCTCTT ACGTTATGCC CAGACTCAAG GCACAGGAGG GAAGAAAGTC
ATTCAGACTG ATGAGTGGAG AAATGGCCCT GTCGAAGAAC GCCTTGAGTA TGCCCTTGTG
AAGGGCATTG AAAAACATAT TATTGAGGAT ACTGAGGAAG CCAGGTTAAA CCAAAAAAAA
TATCCCCGAC CTCTCAATAT AATTGAAGGA CCCCTGATGA ATGGAATGAA AATTGTTGGT
GATCTTTTTG GAGCTGGAAA AATGTTTCTA CCTCAGGTTA TAAAGTCAGC CCGGGTTATG
AAGAAGGCTG TTGGCCACCT TATCCCTTTC ATGGAAAAAG AAAGAGAAGA AACCAGAGTG
CTTAACGGCA CAGTAGAAGA AGAGGACCCT TACCAGGGCA CCATCGTGCT GGCCACTGTT
AAAGGCGACG TGCACGACAT AGGCAAGAAC ATAGTTGGAG TAGTCCTTGG CTGCAATAAT
TTCCGAGTTA TTGATTTAGG AGTCATGACT CCATGTGATA AGATACTGAA AGCTGCTCTT
GACCACAAAG CAGATATAAT TGGCCTGTCA GGACTCATCA CTCCTTCCCT GGATGAAATG
ATTTTTGTTG CCAAGGAAAT GGAGAGATTA GCTATAAGGA TTCCATTGTT GATTGGAGGA
GCAACCACTT CAAAAACCCA CACAGCAGTT AAAATAGCTC CGAGATACAG TGCACCTGTA
ATCCATGTCC TGGACGCGTC CAAGAGTGTG GTGGTGTGTT CCCAGCTGTT AGATGAAAAT
CTAAAGGATG AATACTTTGA GGAAATCATG GAAGAATATG AAGATATTAG ACAGGACCAT
TATGAGTCTC TCAAGGAGAG GAGATACTTA CCCTTAAGTC AAGCCAGAAA AAGTGGTTTC
CAAATGGATT GGCTGTCTGA ACCTCACCCA GTGAAGCCCA CGTTTATTGG GACCCAGGTC
TTTGAAGACT ATGACCTGCA GAAGCTGGTG GACTACATTG ACTGGAAGCC TTTCTTTGAT
GTCTGGCAGC TCCGGGGCAA GTACCCGAAT CGAGGCTTTC CCAAGATATT TAACGACAAA
ACAGTAGGTG GAGAGGCCAG GAAGGTCTAC GATGATGCCC ACAATATGCT GAACACACTG
ATTAGTCAAA AGAAACTCCG GGCCCGGGGT GTGGTTGGGT TCTGGCCAGC ACAGAGTATC
CAAGACGACA TTCACCTGTA CGCAGAGGCT GCTGTGCCCC AGGCTGCAGA GCCCATAGCC
ACCTTCTATG GGTTAAGGCA ACAGGCTGAG AAGGACTCTG CCAGCACGGA GCCATACTAC
TGCCTCTCAG ACTTCATCGC TCCCTTGCAT TCTGGCATCC GTGACTACCT GGGCCTGTTT
GCCGTTGCCT GCTTTGGGGT AGAAGAGCTG AGCAAGGCCT ATGAGGATGA TGGTGACGAC
TACAGCAGCA TCATGGTCAA GGCGCTGGGG GACCGGCTGG CAGAGGCCTT TGCAGAAGAG
CTCCATGAAA GAGTTCGCCG AGAACTGTGG GCCTACTGTG GCAGTGAGCA GCTGGACGTC
GCAGACCTGC GCAGGCTGCG GTACAAGGGC ATCCGCCCGG CTCCTGGCTA CCCCAGCCAG
CCCGACCACA CCGAGAAGCT CACCATGTGG AGACTCGCAG ACATCGAGCA GTCTACAGGC
ATTAGGTTAA CAGAATCATT AGCAATGGCA CCTGCTTCAG CAGTCTCAGG CCTCTACTTC
TCCAATTTGA AGTCCAAATA TTTTGCTGTG GGGAAGATTT CCAAGGATCA GGTTGAGGAT
TATGCATTGA GGAAGAACAT ATCTGTGGCT GAGGTTGAGA AATGGCTTGG ACCCATTTTG
GGATATGATA CAGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25221
Clone ID Related Accession (Same CDS sequence) NM_001291939
Accession Version NM_001291939.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3645bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product methionine synthase isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC377743.1, BC144095.1, AL359259.18 and CB241165.1. On May 10, 2014 this sequence version replaced gi:578802220. Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## regulatory uORF :: PMID: 17683808 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC144095.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGTCACCCG CGCTCCAAGA CCTGTCGCAA CCCGAAGGTC TGAAGAAAAC CCTGCGGGAT 
GAGATCAATG CCATTCTGCA GAAGAGGATT ATGGTGCTGG ATGGAGGGAT GGGGACCATG
ATCCAGCGGG AGAAGCTAAA CGAAGAACAC TTCCGAGGTC AGGAATTTAA AGATCATGCC
AGGCCGCTGA AAGGCAACAA TGACATTTTA AGTATAACTC AGCCTGATGT CATTTACCAA
ATCCATAAGG AATACTTGCT GGCTGGGGCA GATATCATTG AAACAAATAC TTTTAGCAGC
ACTAGTATTG CCCAAGCTGA CTATGGCCTT GAACACTTGG CCTACCGGAT GAACATGTGC
TCTGCAGGAG TGGCCAGAAA AGCTGCCGAG GAGGTAACTC TCCAGACAGG AATTAAGAGG
TTTGTGGCAG GGGCTCTGGG TCCGACTAAT AAGACACTCT CTGTGTCCCC ATCTGTGGAA
AGGCCGGATT ATAGGAACAT CACATTTGAT GAGCTTGTTG AAGCATACCA AGAGCAGGCC
AAAGGACTTC TGGATGGCGG GGTTGATATC TTACTCATTG AAACTATTTT TGATACTGCC
AATGCCAAGG CAGCCTTGTT TGCACTCCAA AATCTTTTTG AGGAGAAATA TGCTCCCCGG
CCTATCTTTA TTTCAGGGAC GATCGTTGAT AAAAGTGGGC GGACTCTTTC CGGACAGACA
GGAGAGGGAT TTGTCATCAG CGTGTCTCAT GGAGAACCAC TCTGCATTGG ATTAAATTGT
GCTTTGGGTG CAGCTGAAAT GAGACCTTTT ATTGAAATAA TTGGAAAATG TACAACAGCC
TATGTCCTCT GTTATCCCAA TGCAGGTCTT CCCAACACCT TTGGTGACTA TGATGAAACG
CCTTCTATGA TGGCCAAGCA CCTAAAGGAT TTTGCTATGG ATGGCTTGGT CAATATAGTT
GGAGGATGCT GTGGGTCAAC ACCAGATCAT ATCAGGGAAA TTGCTGAAGC TGTGAAAAAT
TGTAAGCCTA GAGTTCCACC TGCCACTGCT TTTGAAGGAC ATATGTTACT GTCTGGTCTA
GAGCCCTTCA GGATTGGACC GTACACCAAC TTTGTTAACA TTGGAGAGCG CTGTAATGTT
GCAGGATCAA GGAAGTTTGC TAAACTCATC ATGGCAGGAA ACTATGAAGA AGCCTTGTGT
GTTGCCAAAG TGCAGGTGGA AATGGGAGCC CAGGTGTTGG ATGTCAACAT GGATGATGGC
ATGCTAGATG GTCCAAGTGC AATGACCAGA TTTTGCAACT TAATTGCTTC CGAGCCAGAC
ATCGCAAAGG TACCTTTGTG CATCGACTCC TCCAATTTTG CTGTGATTGA AGCTGGGTTA
AAGTGCTGCC AAGGGAAGTG CATTGTCAAT AGCATTAGTC TGAAGGAAGG AGAGGACGAC
TTCTTGGAGA AGGCCAGGAA GATTAAAAAG TATGGAGCTG CTATGGTGGT CATGGCTTTT
GATGAAGAAG GACAGGCAAC AGAAACAGAC ACAAAAATCA GAGTGTGCAC CCGGGCCTAC
CATCTGCTTG TGAAAAAACT GGGCTTTAAT CCAAATGACA TTATTTTTGA CCCTAATATC
CTAACCATTG GGACTGGAAT GGAGGAACAC AACTTGTATG CCATTAATTT TATCCATGCA
ACAAAAGTCA TTAAAGAAAC ATTACCTGGA GCCAGAATAA GTGGAGGTCT TTCCAACTTG
TCCTTCTCCT TCCGAGGAAT GGAAGCCATT CGAGAAGCAA TGCATGGGGT TTTCCTTTAC
CATGCAATCA AGTCTGGCAT GGACATGGGG ATAGTGAATG CTGGAAACCT CCCTGTGTAT
GATGATATCC ATAAGGAACT TCTGCAGCTC TGTGAAGATC TCATCTGGAA TAAAGACCCT
GAGGCCACTG AGAAGCTCTT ACGTTATGCC CAGACTCAAG GCACAGGAGG GAAGAAAGTC
ATTCAGACTG ATGAGTGGAG AAATGGCCCT GTCGAAGAAC GCCTTGAGTA TGCCCTTGTG
AAGGTTATAA AGTCAGCCCG GGTTATGAAG AAGGCTGTTG GCCACCTTAT CCCTTTCATG
GAAAAAGAAA GAGAAGAAAC CAGAGTGCTT AACGGCACAG TAGAAGAAGA GGACCCTTAC
CAGGGCACCA TCGTGCTGGC CACTGTTAAA GGCGACGTGC ACGACATAGG CAAGAACATA
GTTGGAGTAG TCCTTGGCTG CAATAATTTC CGAGTTATTG ATTTAGGAGT CATGACTCCA
TGTGATAAGA TACTGAAAGC TGCTCTTGAC CACAAAGCAG ATATAATTGG CCTGTCAGGA
CTCATCACTC CTTCCCTGGA TGAAATGATT TTTGTTGCCA AGGAAATGGA GAGATTAGCT
ATAAGGATTC CATTGTTGAT TGGAGGAGCA ACCACTTCAA AAACCCACAC AGCAGTTAAA
ATAGCTCCGA GATACAGTGC ACCTGTAATC CATGTCCTGG ACGCGTCCAA GAGTGTGGTG
GTGTGTTCCC AGCTGTTAGA TGAAAATCTA AAGGATGAAT ACTTTGAGGA AATCATGGAA
GAATATGAAG ATATTAGACA GGACCATTAT GAGTCTCTCA AGGAGAGGAG ATACTTACCC
TTAAGTCAAG CCAGAAAAAG TGGTTTCCAA ATGGATTGGC TGTCTGAACC TCACCCAGTG
AAGCCCACGT TTATTGGGAC CCAGGTCTTT GAAGACTATG ACCTGCAGAA GCTGGTGGAC
TACATTGACT GGAAGCCTTT CTTTGATGTC TGGCAGCTCC GGGGCAAGTA CCCGAATCGA
GGCTTTCCCA AGATATTTAA CGACAAAACA GTAGGTGGAG AGGCCAGGAA GGTCTACGAT
GATGCCCACA ATATGCTGAA CACACTGATT AGTCAAAAGA AACTCCGGGC CCGGGGTGTG
GTTGGGTTCT GGCCAGCACA GAGTATCCAA GACGACATTC ACCTGTACGC AGAGGCTGCT
GTGCCCCAGG CTGCAGAGCC CATAGCCACC TTCTATGGGT TAAGGCAACA GGCTGAGAAG
GACTCTGCCA GCACGGAGCC ATACTACTGC CTCTCAGACT TCATCGCTCC CTTGCATTCT
GGCATCCGTG ACTACCTGGG CCTGTTTGCC GTTGCCTGCT TTGGGGTAGA AGAGCTGAGC
AAGGCCTATG AGGATGATGG TGACGACTAC AGCAGCATCA TGGTCAAGGC GCTGGGGGAC
CGGCTGGCAG AGGCCTTTGC AGAAGAGCTC CATGAAAGAG TTCGCCGAGA ACTGTGGGCC
TACTGTGGCA GTGAGCAGCT GGACGTCGCA GACCTGCGCA GGCTGCGGTA CAAGGGCATC
CGCCCGGCTC CTGGCTACCC CAGCCAGCCC GACCACACCG AGAAGCTCAC CATGTGGAGA
CTCGCAGACA TCGAGCAGTC TACAGGCATT AGGTTAACAG AATCATTAGC AATGGCACCT
GCTTCAGCAG TCTCAGGCCT CTACTTCTCC AATTTGAAGT CCAAATATTT TGCTGTGGGG
AAGATTTCCA AGGATCAGGT TGAGGATTAT GCATTGAGGA AGAACATATC TGTGGCTGAG
GTTGAGAAAT GGCTTGGACC CATTTTGGGA TATGATACAG ACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001278868.1
CDS424..4068
Misc Feature(1)41..466(+)
Misc Feature(2)64..156(+)
Misc Feature(3)238..240(+)
Misc Feature(4)478..4053(+)
Misc Feature(5)484..1443(+)
Misc Feature(6)1534..2304(+)
Misc Feature(7)1558..2262(+)
Misc Feature(8)2458..2961(+)
Misc Feature(9)2752..2937(+)
Misc Feature(10)3160..4011(+)
Exon (1)1..457
Gene:MTR
Gene Synonym:
Exon (2)458..672
Gene:MTR
Gene Synonym:
Exon (3)673..762
Gene:MTR
Gene Synonym:
Exon (4)763..832
Gene:MTR
Gene Synonym:
Exon (5)833..925
Gene:MTR
Gene Synonym:
Exon (6)926..1032
Gene:MTR
Gene Synonym:
Exon (7)1033..1092
Gene:MTR
Gene Synonym:
Exon (8)1093..1187
Gene:MTR
Gene Synonym:
Exon (9)1188..1288
Gene:MTR
Gene Synonym:
Exon (10)1289..1350
Gene:MTR
Gene Synonym:
Exon (11)1351..1418
Gene:MTR
Gene Synonym:
Exon (12)1419..1498
Gene:MTR
Gene Synonym:
Exon (13)1499..1611
Gene:MTR
Gene Synonym:
Exon (14)1612..1752
Gene:MTR
Gene Synonym:
Exon (15)1753..1938
Gene:MTR
Gene Synonym:
Exon (16)1939..2118
Gene:MTR
Gene Synonym:
Exon (17)2119..2235
Gene:MTR
Gene Synonym:
Exon (18)2236..2376
Gene:MTR
Gene Synonym:
Exon (19)2377..2466
Gene:MTR
Gene Synonym:
Exon (20)2467..2574
Gene:MTR
Gene Synonym:
Exon (21)2575..2675
Gene:MTR
Gene Synonym:
Exon (22)2676..2743
Gene:MTR
Gene Synonym:
Exon (23)2744..2864
Gene:MTR
Gene Synonym:
Exon (24)2865..2946
Gene:MTR
Gene Synonym:
Exon (25)2947..3045
Gene:MTR
Gene Synonym:
Exon (26)3046..3121
Gene:MTR
Gene Synonym:
Exon (27)3122..3277
Gene:MTR
Gene Synonym:
Exon (28)3278..3474
Gene:MTR
Gene Synonym:
Exon (29)3475..3675
Gene:MTR
Gene Synonym:
Exon (30)3676..3868
Gene:MTR
Gene Synonym:
Exon (31)3869..3981
Gene:MTR
Gene Synonym:
Exon (32)3982..10405
Gene:MTR
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001291939
Organism Homo sapiens (human)
Definition Homo sapiens 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001291939

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGTCACCCG CGCTCCAAGA CCTGTCGCAA CCCGAAGGTC TGAAGAAAAC CCTGCGGGAT 
GAGATCAATG CCATTCTGCA GAAGAGGATT ATGGTGCTGG ATGGAGGGAT GGGGACCATG
ATCCAGCGGG AGAAGCTAAA CGAAGAACAC TTCCGAGGTC AGGAATTTAA AGATCATGCC
AGGCCGCTGA AAGGCAACAA TGACATTTTA AGTATAACTC AGCCTGATGT CATTTACCAA
ATCCATAAGG AATACTTGCT GGCTGGGGCA GATATCATTG AAACAAATAC TTTTAGCAGC
ACTAGTATTG CCCAAGCTGA CTATGGCCTT GAACACTTGG CCTACCGGAT GAACATGTGC
TCTGCAGGAG TGGCCAGAAA AGCTGCCGAG GAGGTAACTC TCCAGACAGG AATTAAGAGG
TTTGTGGCAG GGGCTCTGGG TCCGACTAAT AAGACACTCT CTGTGTCCCC ATCTGTGGAA
AGGCCGGATT ATAGGAACAT CACATTTGAT GAGCTTGTTG AAGCATACCA AGAGCAGGCC
AAAGGACTTC TGGATGGCGG GGTTGATATC TTACTCATTG AAACTATTTT TGATACTGCC
AATGCCAAGG CAGCCTTGTT TGCACTCCAA AATCTTTTTG AGGAGAAATA TGCTCCCCGG
CCTATCTTTA TTTCAGGGAC GATCGTTGAT AAAAGTGGGC GGACTCTTTC CGGACAGACA
GGAGAGGGAT TTGTCATCAG CGTGTCTCAT GGAGAACCAC TCTGCATTGG ATTAAATTGT
GCTTTGGGTG CAGCTGAAAT GAGACCTTTT ATTGAAATAA TTGGAAAATG TACAACAGCC
TATGTCCTCT GTTATCCCAA TGCAGGTCTT CCCAACACCT TTGGTGACTA TGATGAAACG
CCTTCTATGA TGGCCAAGCA CCTAAAGGAT TTTGCTATGG ATGGCTTGGT CAATATAGTT
GGAGGATGCT GTGGGTCAAC ACCAGATCAT ATCAGGGAAA TTGCTGAAGC TGTGAAAAAT
TGTAAGCCTA GAGTTCCACC TGCCACTGCT TTTGAAGGAC ATATGTTACT GTCTGGTCTA
GAGCCCTTCA GGATTGGACC GTACACCAAC TTTGTTAACA TTGGAGAGCG CTGTAATGTT
GCAGGATCAA GGAAGTTTGC TAAACTCATC ATGGCAGGAA ACTATGAAGA AGCCTTGTGT
GTTGCCAAAG TGCAGGTGGA AATGGGAGCC CAGGTGTTGG ATGTCAACAT GGATGATGGC
ATGCTAGATG GTCCAAGTGC AATGACCAGA TTTTGCAACT TAATTGCTTC CGAGCCAGAC
ATCGCAAAGG TACCTTTGTG CATCGACTCC TCCAATTTTG CTGTGATTGA AGCTGGGTTA
AAGTGCTGCC AAGGGAAGTG CATTGTCAAT AGCATTAGTC TGAAGGAAGG AGAGGACGAC
TTCTTGGAGA AGGCCAGGAA GATTAAAAAG TATGGAGCTG CTATGGTGGT CATGGCTTTT
GATGAAGAAG GACAGGCAAC AGAAACAGAC ACAAAAATCA GAGTGTGCAC CCGGGCCTAC
CATCTGCTTG TGAAAAAACT GGGCTTTAAT CCAAATGACA TTATTTTTGA CCCTAATATC
CTAACCATTG GGACTGGAAT GGAGGAACAC AACTTGTATG CCATTAATTT TATCCATGCA
ACAAAAGTCA TTAAAGAAAC ATTACCTGGA GCCAGAATAA GTGGAGGTCT TTCCAACTTG
TCCTTCTCCT TCCGAGGAAT GGAAGCCATT CGAGAAGCAA TGCATGGGGT TTTCCTTTAC
CATGCAATCA AGTCTGGCAT GGACATGGGG ATAGTGAATG CTGGAAACCT CCCTGTGTAT
GATGATATCC ATAAGGAACT TCTGCAGCTC TGTGAAGATC TCATCTGGAA TAAAGACCCT
GAGGCCACTG AGAAGCTCTT ACGTTATGCC CAGACTCAAG GCACAGGAGG GAAGAAAGTC
ATTCAGACTG ATGAGTGGAG AAATGGCCCT GTCGAAGAAC GCCTTGAGTA TGCCCTTGTG
AAGGTTATAA AGTCAGCCCG GGTTATGAAG AAGGCTGTTG GCCACCTTAT CCCTTTCATG
GAAAAAGAAA GAGAAGAAAC CAGAGTGCTT AACGGCACAG TAGAAGAAGA GGACCCTTAC
CAGGGCACCA TCGTGCTGGC CACTGTTAAA GGCGACGTGC ACGACATAGG CAAGAACATA
GTTGGAGTAG TCCTTGGCTG CAATAATTTC CGAGTTATTG ATTTAGGAGT CATGACTCCA
TGTGATAAGA TACTGAAAGC TGCTCTTGAC CACAAAGCAG ATATAATTGG CCTGTCAGGA
CTCATCACTC CTTCCCTGGA TGAAATGATT TTTGTTGCCA AGGAAATGGA GAGATTAGCT
ATAAGGATTC CATTGTTGAT TGGAGGAGCA ACCACTTCAA AAACCCACAC AGCAGTTAAA
ATAGCTCCGA GATACAGTGC ACCTGTAATC CATGTCCTGG ACGCGTCCAA GAGTGTGGTG
GTGTGTTCCC AGCTGTTAGA TGAAAATCTA AAGGATGAAT ACTTTGAGGA AATCATGGAA
GAATATGAAG ATATTAGACA GGACCATTAT GAGTCTCTCA AGGAGAGGAG ATACTTACCC
TTAAGTCAAG CCAGAAAAAG TGGTTTCCAA ATGGATTGGC TGTCTGAACC TCACCCAGTG
AAGCCCACGT TTATTGGGAC CCAGGTCTTT GAAGACTATG ACCTGCAGAA GCTGGTGGAC
TACATTGACT GGAAGCCTTT CTTTGATGTC TGGCAGCTCC GGGGCAAGTA CCCGAATCGA
GGCTTTCCCA AGATATTTAA CGACAAAACA GTAGGTGGAG AGGCCAGGAA GGTCTACGAT
GATGCCCACA ATATGCTGAA CACACTGATT AGTCAAAAGA AACTCCGGGC CCGGGGTGTG
GTTGGGTTCT GGCCAGCACA GAGTATCCAA GACGACATTC ACCTGTACGC AGAGGCTGCT
GTGCCCCAGG CTGCAGAGCC CATAGCCACC TTCTATGGGT TAAGGCAACA GGCTGAGAAG
GACTCTGCCA GCACGGAGCC ATACTACTGC CTCTCAGACT TCATCGCTCC CTTGCATTCT
GGCATCCGTG ACTACCTGGG CCTGTTTGCC GTTGCCTGCT TTGGGGTAGA AGAGCTGAGC
AAGGCCTATG AGGATGATGG TGACGACTAC AGCAGCATCA TGGTCAAGGC GCTGGGGGAC
CGGCTGGCAG AGGCCTTTGC AGAAGAGCTC CATGAAAGAG TTCGCCGAGA ACTGTGGGCC
TACTGTGGCA GTGAGCAGCT GGACGTCGCA GACCTGCGCA GGCTGCGGTA CAAGGGCATC
CGCCCGGCTC CTGGCTACCC CAGCCAGCCC GACCACACCG AGAAGCTCAC CATGTGGAGA
CTCGCAGACA TCGAGCAGTC TACAGGCATT AGGTTAACAG AATCATTAGC AATGGCACCT
GCTTCAGCAG TCTCAGGCCT CTACTTCTCC AATTTGAAGT CCAAATATTT TGCTGTGGGG
AAGATTTCCA AGGATCAGGT TGAGGATTAT GCATTGAGGA AGAACATATC TGTGGCTGAG
GTTGAGAAAT GGCTTGGACC CATTTTGGGA TATGATACAG ACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25092
Clone ID Related Accession (Same CDS sequence) NM_001291940
Accession Version NM_001291940.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product methionine synthase isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC377743.1, BC144098.1, BC136440.1, AL359259.18 and CB241165.1. Transcript Variant: This variant (3) lacks an internal exon, which causes translation initiation at a downstream AUG, compared to variant 1. The resulting isoform (3) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144098.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGGGAGCCC AGGTGTTGGA TGTCAACATG GATGATGGCA TGCTAGATGG TCCAAGTGCA 
ATGACCAGAT TTTGCAACTT AATTGCTTCC GAGCCAGACA TCGCAAAGGT ACCTTTGTGC
ATCGACTCCT CCAATTTTGC TGTGATTGAA GCTGGGTTAA AGTGCTGCCA AGGGAAGTGC
ATTGTCAATA GCATTAGTCT GAAGGAAGGA GAGGACGACT TCTTGGAGAA GGCCAGGAAG
ATTAAAAAGT ATGGAGCTGC TATGGTGGTC ATGGCTTTTG ATGAAGAAGG ACAGGCAACA
GAAACAGACA CAAAAATCAG AGTGTGCACC CGGGCCTACC ATCTGCTTGT GAAAAAACTG
GGCTTTAATC CAAATGACAT TATTTTTGAC CCTAATATCC TAACCATTGG GACTGGAATG
GAGGAACACA ACTTGTATGC CATTAATTTT ATCCATGCAA CAAAAGTCAT TAAAGAAACA
TTACCTGGAG CCAGAATAAG TGGAGGTCTT TCCAACTTGT CCTTCTCCTT CCGAGGAATG
GAAGCCATTC GAGAAGCAAT GCATGGGGTT TTCCTTTACC ATGCAATCAA GTCTGGCATG
GACATGGGGA TAGTGAATGC TGGAAACCTC CCTGTGTATG ATGATATCCA TAAGGAACTT
CTGCAGCTCT GTGAAGATCT CATCTGGAAT AAAGACCCTG AGGCCACTGA GAAGCTCTTA
CGTTATGCCC AGACTCAAGG CACAGGAGGG AAGAAAGTCA TTCAGACTGA TGAGTGGAGA
AATGGCCCTG TCGAAGAACG CCTTGAGTAT GCCCTTGTGA AGGGCATTGA AAAACATATT
ATTGAGGATA CTGAGGAAGC CAGGTTAAAC CAAAAAAAAT ATCCCCGACC TCTCAATATA
ATTGAAGGAC CCCTGATGAA TGGAATGAAA ATTGTTGGTG ATCTTTTTGG AGCTGGAAAA
ATGTTTCTAC CTCAGGTTAT AAAGTCAGCC CGGGTTATGA AGAAGGCTGT TGGCCACCTT
ATCCCTTTCA TGGAAAAAGA AAGAGAAGAA ACCAGAGTGC TTAACGGCAC AGTAGAAGAA
GAGGACCCTT ACCAGGGCAC CATCGTGCTG GCCACTGTTA AAGGCGACGT GCACGACATA
GGCAAGAACA TAGTTGGAGT AGTCCTTGGC TGCAATAATT TCCGAGTTAT TGATTTAGGA
GTCATGACTC CATGTGATAA GATACTGAAA GCTGCTCTTG ACCACAAAGC AGATATAATT
GGCCTGTCAG GACTCATCAC TCCTTCCCTG GATGAAATGA TTTTTGTTGC CAAGGAAATG
GAGAGATTAG CTATAAGGAT TCCATTGTTG ATTGGAGGAG CAACCACTTC AAAAACCCAC
ACAGCAGTTA AAATAGCTCC GAGATACAGT GCACCTGTAA TCCATGTCCT GGACGCGTCC
AAGAGTGTGG TGGTGTGTTC CCAGCTGTTA GATGAAAATC TAAAGGATGA ATACTTTGAG
GAAATCATGG AAGAATATGA AGATATTAGA CAGGACCATT ATGAGTCTCT CAAGGAGAGG
AGATACTTAC CCTTAAGTCA AGCCAGAAAA AGTGGTTTCC AAATGGATTG GCTGTCTGAA
CCTCACCCAG TGAAGCCCAC GTTTATTGGG ACCCAGGTCT TTGAAGACTA TGACCTGCAG
AAGCTGGTGG ACTACATTGA CTGGAAGCCT TTCTTTGATG TCTGGCAGCT CCGGGGCAAG
TACCCGAATC GAGGCTTTCC CAAGATATTT AACGACAAAA CAGTAGGTGG AGAGGCCAGG
AAGGTCTACG ATGATGCCCA CAATATGCTG AACACACTGA TTAGTCAAAA GAAACTCCGG
GCCCGGGGTG TGGTTGGGTT CTGGCCAGCA CAGAGTATCC AAGACGACAT TCACCTGTAC
GCAGAGGCTG CTGTGCCCCA GGCTGCAGAG CCCATAGCCA CCTTCTATGG GTTAAGGCAA
CAGGCTGAGA AGGACTCTGC CAGCACGGAG CCATACTACT GCCTCTCAGA CTTCATCGCT
CCCTTGCATT CTGGCATCCG TGACTACCTG GGCCTGTTTG CCGTTGCCTG CTTTGGGGTA
GAAGAGCTGA GCAAGGCCTA TGAGGATGAT GGTGACGACT ACAGCAGCAT CATGGTCAAG
GCGCTGGGGG ACCGGCTGGC AGAGGCCTTT GCAGAAGAGC TCCATGAAAG AGTTCGCCGA
GAACTGTGGG CCTACTGTGG CAGTGAGCAG CTGGACGTCG CAGACCTGCG CAGGCTGCGG
TACAAGGGCA TCCGCCCGGC TCCTGGCTAC CCCAGCCAGC CCGACCACAC CGAGAAGCTC
ACCATGTGGA GACTCGCAGA CATCGAGCAG TCTACAGGCA TTAGGTTAAC AGAATCATTA
GCAATGGCAC CTGCTTCAGC AGTCTCAGGC CTCTACTTCT CCAATTTGAA GTCCAAATAT
TTTGCTGTGG GGAAGATTTC CAAGGATCAG GTTGAGGATT ATGCATTGAG GAAGAACATA
TCTGTGGCTG AGGTTGAGAA ATGGCTTGGA CCCATTTTGG GATATGATAC AGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001278869.1
CDS1532..4108
Misc Feature(1)1436..1438(+)
Misc Feature(2)1532..2191(+)
Misc Feature(3)1535..4000(+)
Misc Feature(4)1655..2149(+)
Misc Feature(5)2321..3001(+)
Misc Feature(6)2435..2977(+)
Misc Feature(7)2663..2665(+)
Misc Feature(8)3200..4051(+)
Exon (1)1..457
Gene:MTR
Gene Synonym:
Exon (2)458..672
Gene:MTR
Gene Synonym:
Exon (3)673..762
Gene:MTR
Gene Synonym:
Exon (4)763..832
Gene:MTR
Gene Synonym:
Exon (5)833..925
Gene:MTR
Gene Synonym:
Exon (6)926..1032
Gene:MTR
Gene Synonym:
Exon (7)1033..1092
Gene:MTR
Gene Synonym:
Exon (8)1093..1187
Gene:MTR
Gene Synonym:
Exon (9)1188..1288
Gene:MTR
Gene Synonym:
Exon (10)1289..1350
Gene:MTR
Gene Synonym:
Exon (11)1351..1418
Gene:MTR
Gene Synonym:
Exon (12)1419..1498
Gene:MTR
Gene Synonym:
Exon (13)1499..1639
Gene:MTR
Gene Synonym:
Exon (14)1640..1825
Gene:MTR
Gene Synonym:
Exon (15)1826..2005
Gene:MTR
Gene Synonym:
Exon (16)2006..2122
Gene:MTR
Gene Synonym:
Exon (17)2123..2263
Gene:MTR
Gene Synonym:
Exon (18)2264..2353
Gene:MTR
Gene Synonym:
Exon (19)2354..2506
Gene:MTR
Gene Synonym:
Exon (20)2507..2614
Gene:MTR
Gene Synonym:
Exon (21)2615..2715
Gene:MTR
Gene Synonym:
Exon (22)2716..2783
Gene:MTR
Gene Synonym:
Exon (23)2784..2904
Gene:MTR
Gene Synonym:
Exon (24)2905..2986
Gene:MTR
Gene Synonym:
Exon (25)2987..3085
Gene:MTR
Gene Synonym:
Exon (26)3086..3161
Gene:MTR
Gene Synonym:
Exon (27)3162..3317
Gene:MTR
Gene Synonym:
Exon (28)3318..3514
Gene:MTR
Gene Synonym:
Exon (29)3515..3715
Gene:MTR
Gene Synonym:
Exon (30)3716..3908
Gene:MTR
Gene Synonym:
Exon (31)3909..4021
Gene:MTR
Gene Synonym:
Exon (32)4022..10445
Gene:MTR
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001291940
Organism Homo sapiens (human)
Definition Homo sapiens 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001291940

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGGGAGCCC AGGTGTTGGA TGTCAACATG GATGATGGCA TGCTAGATGG TCCAAGTGCA 
ATGACCAGAT TTTGCAACTT AATTGCTTCC GAGCCAGACA TCGCAAAGGT ACCTTTGTGC
ATCGACTCCT CCAATTTTGC TGTGATTGAA GCTGGGTTAA AGTGCTGCCA AGGGAAGTGC
ATTGTCAATA GCATTAGTCT GAAGGAAGGA GAGGACGACT TCTTGGAGAA GGCCAGGAAG
ATTAAAAAGT ATGGAGCTGC TATGGTGGTC ATGGCTTTTG ATGAAGAAGG ACAGGCAACA
GAAACAGACA CAAAAATCAG AGTGTGCACC CGGGCCTACC ATCTGCTTGT GAAAAAACTG
GGCTTTAATC CAAATGACAT TATTTTTGAC CCTAATATCC TAACCATTGG GACTGGAATG
GAGGAACACA ACTTGTATGC CATTAATTTT ATCCATGCAA CAAAAGTCAT TAAAGAAACA
TTACCTGGAG CCAGAATAAG TGGAGGTCTT TCCAACTTGT CCTTCTCCTT CCGAGGAATG
GAAGCCATTC GAGAAGCAAT GCATGGGGTT TTCCTTTACC ATGCAATCAA GTCTGGCATG
GACATGGGGA TAGTGAATGC TGGAAACCTC CCTGTGTATG ATGATATCCA TAAGGAACTT
CTGCAGCTCT GTGAAGATCT CATCTGGAAT AAAGACCCTG AGGCCACTGA GAAGCTCTTA
CGTTATGCCC AGACTCAAGG CACAGGAGGG AAGAAAGTCA TTCAGACTGA TGAGTGGAGA
AATGGCCCTG TCGAAGAACG CCTTGAGTAT GCCCTTGTGA AGGGCATTGA AAAACATATT
ATTGAGGATA CTGAGGAAGC CAGGTTAAAC CAAAAAAAAT ATCCCCGACC TCTCAATATA
ATTGAAGGAC CCCTGATGAA TGGAATGAAA ATTGTTGGTG ATCTTTTTGG AGCTGGAAAA
ATGTTTCTAC CTCAGGTTAT AAAGTCAGCC CGGGTTATGA AGAAGGCTGT TGGCCACCTT
ATCCCTTTCA TGGAAAAAGA AAGAGAAGAA ACCAGAGTGC TTAACGGCAC AGTAGAAGAA
GAGGACCCTT ACCAGGGCAC CATCGTGCTG GCCACTGTTA AAGGCGACGT GCACGACATA
GGCAAGAACA TAGTTGGAGT AGTCCTTGGC TGCAATAATT TCCGAGTTAT TGATTTAGGA
GTCATGACTC CATGTGATAA GATACTGAAA GCTGCTCTTG ACCACAAAGC AGATATAATT
GGCCTGTCAG GACTCATCAC TCCTTCCCTG GATGAAATGA TTTTTGTTGC CAAGGAAATG
GAGAGATTAG CTATAAGGAT TCCATTGTTG ATTGGAGGAG CAACCACTTC AAAAACCCAC
ACAGCAGTTA AAATAGCTCC GAGATACAGT GCACCTGTAA TCCATGTCCT GGACGCGTCC
AAGAGTGTGG TGGTGTGTTC CCAGCTGTTA GATGAAAATC TAAAGGATGA ATACTTTGAG
GAAATCATGG AAGAATATGA AGATATTAGA CAGGACCATT ATGAGTCTCT CAAGGAGAGG
AGATACTTAC CCTTAAGTCA AGCCAGAAAA AGTGGTTTCC AAATGGATTG GCTGTCTGAA
CCTCACCCAG TGAAGCCCAC GTTTATTGGG ACCCAGGTCT TTGAAGACTA TGACCTGCAG
AAGCTGGTGG ACTACATTGA CTGGAAGCCT TTCTTTGATG TCTGGCAGCT CCGGGGCAAG
TACCCGAATC GAGGCTTTCC CAAGATATTT AACGACAAAA CAGTAGGTGG AGAGGCCAGG
AAGGTCTACG ATGATGCCCA CAATATGCTG AACACACTGA TTAGTCAAAA GAAACTCCGG
GCCCGGGGTG TGGTTGGGTT CTGGCCAGCA CAGAGTATCC AAGACGACAT TCACCTGTAC
GCAGAGGCTG CTGTGCCCCA GGCTGCAGAG CCCATAGCCA CCTTCTATGG GTTAAGGCAA
CAGGCTGAGA AGGACTCTGC CAGCACGGAG CCATACTACT GCCTCTCAGA CTTCATCGCT
CCCTTGCATT CTGGCATCCG TGACTACCTG GGCCTGTTTG CCGTTGCCTG CTTTGGGGTA
GAAGAGCTGA GCAAGGCCTA TGAGGATGAT GGTGACGACT ACAGCAGCAT CATGGTCAAG
GCGCTGGGGG ACCGGCTGGC AGAGGCCTTT GCAGAAGAGC TCCATGAAAG AGTTCGCCGA
GAACTGTGGG CCTACTGTGG CAGTGAGCAG CTGGACGTCG CAGACCTGCG CAGGCTGCGG
TACAAGGGCA TCCGCCCGGC TCCTGGCTAC CCCAGCCAGC CCGACCACAC CGAGAAGCTC
ACCATGTGGA GACTCGCAGA CATCGAGCAG TCTACAGGCA TTAGGTTAAC AGAATCATTA
GCAATGGCAC CTGCTTCAGC AGTCTCAGGC CTCTACTTCT CCAATTTGAA GTCCAAATAT
TTTGCTGTGG GGAAGATTTC CAAGGATCAG GTTGAGGATT ATGCATTGAG GAAGAACATA
TCTGTGGCTG AGGTTGAGAA ATGGCTTGGA CCCATTTTGG GATATGATAC AGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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