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MUC1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MUC1
Entrez Gene ID 4582
Full Name mucin 1, cell surface associated
Synonyms ADMCKD, ADMCKD1, CA 15-3, CD227, EMA, H23AG, KL-6, MAM6, MCD, MCKD, MCKD1, MUC-1, MUC-1/SEC, MUC-1/X, MUC1/ZD, PEM, PEMT, PUM
General protein information
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q21

Summary This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]. lac of sum
Disorder MIM:

158340

Disorder Html:





The following MUC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MUC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu23074 NM_002456 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$216.30
$309.00
OHu22968 NM_001044392 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.30
$99.00
OHu22660 NM_001044390 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $269.00
OHu22896 NM_001018017 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $269.00
OHu23198 NM_001204285 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $319.00
OHu20146 NM_001018016 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$188.30
$269.00
OHu20062 NM_001204287 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
OHu19896 NM_001204288 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
OHu20181 NM_001204290 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
OHu19920 NM_001204296 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 20, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
OHu19960 NM_001204297 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 21, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99.00
OHu22725 NM_001204293 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 17, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99.00
OHu23033 NM_001204294 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 18, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
OHu21804 NM_001044393 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99.00
OHu20863 NM_001044391 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99.00
OHu21381 NM_001204286 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $319.00
OHu21461 NM_001204289 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
OHu21591 NM_001204291 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 15, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
OHu20867 NM_001204292 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 16, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
OHu21693 NM_001204295 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 19, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu23074
Accession Version NM_002456.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 822bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, U60261.1, AC234582.3 and AA425062.1. This sequence is a reference standard in the RefSeqGene project. On Feb 26, 2011 this sequence version replaced gi:65301116. Summary: This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]. Transcript Variant: This variant (1) represents isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U60261.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTT TGTCTACTGG GGTCTCTTTC
TTTTTCCTGT CTTTTCACAT TTCAAACCTC CAGTTTAATT CCTCTCTGGA AGATCCCAGC
ACCGACTACT ACCAAGAGCT GCAGAGAGAC ATTTCTGAAA TGTTTTTGCA GATTTATAAA
CAAGGGGGTT TTCTGGGCCT CTCCAATATT AAGTTCAGGC CAGGATCTGT GGTGGTACAA
TTGACTCTGG CCTTCCGAGA AGGTACCATC AATGTCCACG ACGTGGAGAC ACAGTTCAAT
CAGTATAAAA CGGAAGCAGC CTCTCGATAT AACCTGACGA TCTCAGACGT CAGCGTGAGT
GATGTGCCAT TTCCTTTCTC TGCCCAGTCT GGGGCTGGGG TGCCAGGCTG GGGCATCGCG
CTGCTGGTGC TGGTCTGTGT TCTGGTTGCG CTGGCCATTG TCTATCTCAT TGCCTTGGCT
GTCTGTCAGT GCCGCCGAAA GAACTACGGG CAGCTGGACA TCTTTCCAGC CCGGGATACC
TACCATCCTA TGAGCGAGTA CCCCACCTAC CACACCCATG GGCGCTATGT GCCCCCTAGC
AGTACCGATC GTAGCCCCTA TGAGAAGGTT TCTGCAGGTA ATGGTGGCAG CAGCCTCTCT
TACACAAACC CAGCAGTGGC AGCCACTTCT GCCAACTTGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_002447.4
CDS73..894
Misc Feature(1)73..141(+)
Misc Feature(2)151..153(+)
Misc Feature(3)235..582(+)
Misc Feature(4)289..291(+)
Misc Feature(5)415..417(+)
Misc Feature(6)523..525(+)
Misc Feature(7)697..699(+)
Misc Feature(8)733..735(+)
Misc Feature(9)751..753(+)
Misc Feature(10)760..762(+)
Misc Feature(11)796..798(+)
Misc Feature(12)805..807(+)
Misc Feature(13)811..813(+)
Misc Feature(14)853..855(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..231
Gene:MUC1
Gene Synonym:
Exon (3)232..354
Gene:MUC1
Gene Synonym:
Exon (4)355..410
Gene:MUC1
Gene Synonym:
Exon (5)411..547
Gene:MUC1
Gene Synonym:
Exon (6)548..669
Gene:MUC1
Gene Synonym:
Exon (7)670..819
Gene:MUC1
Gene Synonym:
Exon (8)820..1205
Gene:MUC1
Gene Synonym:
Translation
Position Chain Variation Link
1 1 c, t dbSNP:562400415
2 2 a, g dbSNP:11465204
20 20 c, t dbSNP:372118515
24 24 c, t dbSNP:368256181
25 25 a, g, t dbSNP:764293615
26 26 c, t dbSNP:760667690
32 32 g, t dbSNP:774386878
33 33 c, g dbSNP:770752618
35 35 a, c dbSNP:749183212
39 39 a, g dbSNP:772996200
41 41 -, t dbSNP:753898556
46 46 c, t dbSNP:769518985
48 48 c, t dbSNP:564315578
65 65 c, t dbSNP:747855021
77 77 c, t dbSNP:780700800
80 80 c, t dbSNP:376608767
81 81 a, g dbSNP:373505102
87 87 c, t dbSNP:779571432
92 92 c, g dbSNP:368800613
100 100 c, t dbSNP:759009730
103 103 c, t dbSNP:377356836
137 137 c, t dbSNP:139437006
138 138 a, g dbSNP:4072037
140 140 a, g dbSNP:766333007
145 145 a, g dbSNP:773637715
155 155 g, t dbSNP:145108039
158 158 c, t dbSNP:145667707
160 160 a, t dbSNP:776982023
163 163 a, c dbSNP:575065522
169 169 a, g dbSNP:771925604
170 170 a, g dbSNP:201761107
173 173 a, g dbSNP:758452127
176 176 a, g dbSNP:745939858
182 182 c, t dbSNP:201186912
183 183 g, t dbSNP:544640619
185 185 c, g, t dbSNP:577380147
186 186 a, c, g dbSNP:751297222
191 191 a, c dbSNP:766031544
192 192 c, g dbSNP:199568358
195 195 a, g dbSNP:749976676
205 205 g, t dbSNP:764582269
210 210 c, t dbSNP:762318074
213 213 a, c, t dbSNP:145691584
215 215 a, c dbSNP:761000946
216 216 c, t dbSNP:200478078
231 231 c, t dbSNP:772361769
234 234 g, t dbSNP:771498973
238 238 a, c dbSNP:749690550
244 244 a, g, t dbSNP:770126594
247 247 c, t dbSNP:748436056
248 248 c, t dbSNP:781359601
257 257 g, t dbSNP:768665660
258 258 c, t dbSNP:747132214
263 263 c, t dbSNP:534337862
270 270 c, t dbSNP:371485486
279 279 c, t dbSNP:753660394
285 285 a, c, g dbSNP:755973313
295 295 a, t dbSNP:35819649
297 297 c, t dbSNP:767259710
298 298 c, t dbSNP:759068582
300 300 c, g dbSNP:751081819
307 307 c, t dbSNP:146950322
312 312 -, c dbSNP:774368817
316 316 a, g dbSNP:765746637
320 320 a, g dbSNP:763569821
325 325 c, g dbSNP:773704188
328 328 c, g dbSNP:770183823
366 366 g, t dbSNP:745924162
378 378 a, g dbSNP:140871200
398 398 a, g dbSNP:780669552
399 399 c, t dbSNP:370819310
401 401 g, t dbSNP:781095660
411 411 a, g dbSNP:768544711
413 413 -, c dbSNP:777127574
416 416 g, t dbSNP:145224844
418 418 a, t dbSNP:373382247
432 432 a, g dbSNP:139620330
437 437 c, t dbSNP:745467701
447 447 c, t dbSNP:778454369
449 449 g, t dbSNP:756756401
453 453 a, g dbSNP:370391810
455 455 c, g dbSNP:758430992
462 462 c, t dbSNP:764490455
467 467 a, t dbSNP:532835613
472 472 a, g, t dbSNP:144273480
474 474 c, t dbSNP:759892292
475 475 a, g, t dbSNP:1611770
491 491 a, g dbSNP:200411609
492 492 a, t dbSNP:41264913
497 497 a, g dbSNP:773293207
499 499 a, g dbSNP:768505970
503 503 c, g, t dbSNP:775163504
504 504 a, g dbSNP:561379331
507 507 a, g dbSNP:745587505
512 512 a, c dbSNP:766169396
517 517 a, c dbSNP:778505736
518 518 g, t dbSNP:770482054
522 522 c, t dbSNP:748774119
530 530 c, t dbSNP:146141676
531 531 a, g dbSNP:201798179
540 540 a, c, t dbSNP:781731301
541 541 a, g dbSNP:755359583
543 543 c, t dbSNP:751875756
546 546 c, t dbSNP:766729514
547 547 a, g dbSNP:201815857
551 551 a, g dbSNP:11465207
561 561 a, t dbSNP:752680798
581 581 c, g dbSNP:199840128
584 584 a, g dbSNP:369649894
590 590 a, g dbSNP:369608212
591 591 a, g dbSNP:774139080
605 605 -, g dbSNP:761859668
607 607 a, g dbSNP:766143764
609 609 c, t dbSNP:375090196
610 610 a, g, t dbSNP:373081136
611 611 c, t dbSNP:747711729
612 612 a, g dbSNP:776240199
613 613 a, c dbSNP:769289843
619 619 a, g, t dbSNP:780622331
624 624 a, g dbSNP:758786611
625 625 c, g dbSNP:746119110
630 630 c, t dbSNP:779173931
631 631 a, g dbSNP:757373880
641 641 c, t dbSNP:200788986
642 642 a, g dbSNP:764097944
645 645 g, t dbSNP:141460657
649 649 a, g dbSNP:751488017
652 652 a, g dbSNP:766124835
656 656 a, g dbSNP:183700327
657 657 c, t dbSNP:772830298
668 668 c, t dbSNP:199768496
676 676 -, tgtc dbSNP:747704526
686 686 a, g dbSNP:764882391
688 688 c, t dbSNP:761613959
692 692 -, a dbSNP:780480243
694 694 a, g dbSNP:753535070
696 696 c, g dbSNP:763738651
699 699 c, t dbSNP:773917931
700 700 a, g dbSNP:774814129
712 712 a, g dbSNP:772667594
722 722 c, g dbSNP:557552446
724 724 c, t dbSNP:774796958
725 725 a, g dbSNP:771275191
727 727 a, g dbSNP:749581526
745 745 a, c dbSNP:777946901
747 747 c, g, t dbSNP:200639498
748 748 a, g dbSNP:768456378
752 752 a, c dbSNP:781066358
757 757 a, c dbSNP:758368648
760 760 g, t dbSNP:750312588
769 769 c, t dbSNP:778833490
770 770 a, t dbSNP:757088101
775 775 c, t dbSNP:572153326
776 776 a, g dbSNP:763861742
794 794 a, g dbSNP:760364466
798 798 a, c, t dbSNP:766884618
799 799 a, g dbSNP:760149442
802 802 c, g, t dbSNP:372682991
803 803 g, t dbSNP:191544901
812 812 a, g dbSNP:148332231
827 827 c, t dbSNP:773579272
835 835 g, t dbSNP:765571435
836 836 g, t dbSNP:762162139
848 848 c, t dbSNP:776685788
850 850 c, t dbSNP:768830740
852 852 -, tt dbSNP:779449960
855 855 c, t dbSNP:11465209
860 860 a, c dbSNP:775673556
862 862 a, c dbSNP:770998731
866 866 a, c dbSNP:749183591
869 869 c, t dbSNP:777772044
871 871 c, g dbSNP:199968990
872 872 c, g dbSNP:747879087
881 881 c, g dbSNP:780727289
888 888 c, t dbSNP:754615206
900 900 c, t dbSNP:1611772
901 901 a, g dbSNP:549073561
903 903 c, t dbSNP:369597551
904 904 a, g dbSNP:139043100
906 906 a, c dbSNP:765630606
907 907 c, t dbSNP:762060905
916 916 c, g dbSNP:777006162
920 920 a, g dbSNP:764254987
921 921 c, g, t dbSNP:775726708
934 934 a, g dbSNP:772268236
942 942 c, t dbSNP:752708990
944 944 a, g dbSNP:773256715
950 950 g, t dbSNP:769683074
973 973 c, t dbSNP:1611773
1019 1019 a, g dbSNP:779032625
1048 1048 c, t dbSNP:780969195
1049 1049 a, g dbSNP:756031439
1062 1062 c, t dbSNP:768203896
1064 1064 c, t dbSNP:746647874
1091 1091 a, g dbSNP:11465210
1111 1111 g, t dbSNP:750380223
1126 1126 a, t dbSNP:188749315
1138 1138 c, t dbSNP:757872424
1145 1145 a, g dbSNP:750976032
1160 1160 a, g, t dbSNP:112893627
1173 1173 a, g dbSNP:767317412
1201 1201 a, g dbSNP:533295118

Target ORF information:

RefSeq Version NM_002456
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002456

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTT TGTCTACTGG GGTCTCTTTC
TTTTTCCTGT CTTTTCACAT TTCAAACCTC CAGTTTAATT CCTCTCTGGA AGATCCCAGC
ACCGACTACT ACCAAGAGCT GCAGAGAGAC ATTTCTGAAA TGTTTTTGCA GATTTATAAA
CAAGGGGGTT TTCTGGGCCT CTCCAATATT AAGTTCAGGC CAGGATCTGT GGTGGTACAA
TTGACTCTGG CCTTCCGAGA AGGTACCATC AATGTCCACG ACGTGGAGAC ACAGTTCAAT
CAGTATAAAA CGGAAGCAGC CTCTCGATAT AACCTGACGA TCTCAGACGT CAGCGTGAGT
GATGTGCCAT TTCCTTTCTC TGCCCAGTCT GGGGCTGGGG TGCCAGGCTG GGGCATCGCG
CTGCTGGTGC TGGTCTGTGT TCTGGTTGCG CTGGCCATTG TCTATCTCAT TGCCTTGGCT
GTCTGTCAGT GCCGCCGAAA GAACTACGGG CAGCTGGACA TCTTTCCAGC CCGGGATACC
TACCATCCTA TGAGCGAGTA CCCCACCTAC CACACCCATG GGCGCTATGT GCCCCCTAGC
AGTACCGATC GTAGCCCCTA TGAGAAGGTT TCTGCAGGTA ATGGTGGCAG CAGCCTCTCT
TACACAAACC CAGCAGTGGC AGCCACTTCT GCCAACTTGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22968
Accession Version NM_001044392.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 480bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 7 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, AY327584.1, AC234582.3 and AA425062.1. On Feb 26, 2011 this sequence version replaced gi:113206027. Summary: This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]. Transcript Variant: This variant (7) has multiple differences but maintains the reading frame, compared to variant 1. These differences result in a shorter isoform (7), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY327584.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTTA ATTCCTCTCT GGAAGATCCC AGCACCGACT ACTACCAAGA GCTGCAGAGA
GACATTTCTG AAATGGCTGT CTGTCAGTGC CGCCGAAAGA ACTACGGGCA GCTGGACATC
TTTCCAGCCC GGGATACCTA CCATCCTATG AGCGAGTACC CCACCTACCA CACCCATGGG
CGCTATGTGC CCCCTAGCAG TACCGATCGT AGCCCCTATG AGAAGGTTTC TGCAGGTAAT
GGTGGCAGCA GCCTCTCTTA CACAAACCCA GCAGTGGCAG CCACTTCTGC CAACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001037857.1
CDS73..552
Misc Feature(1)205..>327(+)
Misc Feature(2)355..357(+)
Misc Feature(3)391..393(+)
Misc Feature(4)409..411(+)
Misc Feature(5)418..420(+)
Misc Feature(6)427..>534(+)
Misc Feature(7)469..471(+)
Misc Feature(8)511..513(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..258
Gene:MUC1
Gene Synonym:
Exon (3)259..327
Gene:MUC1
Gene Synonym:
Exon (4)328..477
Gene:MUC1
Gene Synonym:
Exon (5)478..863
Gene:MUC1
Gene Synonym:
Translation
Position Chain Variation Link
1 1 c, t dbSNP:562400415
2 2 a, g dbSNP:11465204
20 20 c, t dbSNP:372118515
24 24 c, t dbSNP:368256181
25 25 a, g, t dbSNP:764293615
26 26 c, t dbSNP:760667690
32 32 g, t dbSNP:774386878
33 33 c, g dbSNP:770752618
35 35 a, c dbSNP:749183212
39 39 a, g dbSNP:772996200
41 41 -, t dbSNP:753898556
46 46 c, t dbSNP:769518985
48 48 c, t dbSNP:564315578
65 65 c, t dbSNP:747855021
77 77 c, t dbSNP:780700800
80 80 c, t dbSNP:376608767
81 81 a, g dbSNP:373505102
87 87 c, t dbSNP:779571432
92 92 c, g dbSNP:368800613
100 100 c, t dbSNP:759009730
103 103 c, t dbSNP:377356836
131 131 c, t dbSNP:200001039
136 136 a, c dbSNP:758495827
144 144 a, t dbSNP:753789297
146 146 a, t dbSNP:764061775
148 148 c, g dbSNP:143297835
149 149 c, t dbSNP:752564558
151 151 a, g dbSNP:767382557
152 152 c, g dbSNP:147388624
153 153 a, g dbSNP:774131502
164 164 c, t dbSNP:139437006
165 165 a, g dbSNP:4072037
167 167 a, g dbSNP:766333007
172 172 a, g dbSNP:773637715
182 182 g, t dbSNP:145108039
185 185 c, t dbSNP:145667707
187 187 a, t dbSNP:776982023
190 190 a, c dbSNP:575065522
196 196 a, g dbSNP:771925604
197 197 a, g dbSNP:201761107
200 200 a, g dbSNP:758452127
203 203 a, g dbSNP:745939858
209 209 c, t dbSNP:201186912
210 210 g, t dbSNP:544640619
212 212 c, g, t dbSNP:577380147
213 213 a, c, g dbSNP:751297222
218 218 a, c dbSNP:766031544
219 219 c, g dbSNP:199568358
222 222 a, g dbSNP:749976676
232 232 g, t dbSNP:764582269
237 237 c, t dbSNP:762318074
240 240 a, c, t dbSNP:145691584
242 242 a, c dbSNP:761000946
243 243 c, t dbSNP:200478078
258 258 c, t dbSNP:772361769
268 268 a, t dbSNP:35819649
270 270 c, t dbSNP:767259710
271 271 c, t dbSNP:759068582
273 273 c, g dbSNP:751081819
280 280 c, t dbSNP:146950322
285 285 -, c dbSNP:774368817
289 289 a, g dbSNP:765746637
293 293 a, g dbSNP:763569821
298 298 c, g dbSNP:773704188
301 301 c, g dbSNP:770183823
334 334 -, tgtc dbSNP:747704526
344 344 a, g dbSNP:764882391
346 346 c, t dbSNP:761613959
350 350 -, a dbSNP:780480243
352 352 a, g dbSNP:753535070
354 354 c, g dbSNP:763738651
357 357 c, t dbSNP:773917931
358 358 a, g dbSNP:774814129
370 370 a, g dbSNP:772667594
380 380 c, g dbSNP:557552446
382 382 c, t dbSNP:774796958
383 383 a, g dbSNP:771275191
385 385 a, g dbSNP:749581526
403 403 a, c dbSNP:777946901
405 405 c, g, t dbSNP:200639498
406 406 a, g dbSNP:768456378
410 410 a, c dbSNP:781066358
415 415 a, c dbSNP:758368648
418 418 g, t dbSNP:750312588
427 427 c, t dbSNP:778833490
428 428 a, t dbSNP:757088101
433 433 c, t dbSNP:572153326
434 434 a, g dbSNP:763861742
452 452 a, g dbSNP:760364466
456 456 a, c, t dbSNP:766884618
457 457 a, g dbSNP:760149442
460 460 c, g, t dbSNP:372682991
461 461 g, t dbSNP:191544901
470 470 a, g dbSNP:148332231
485 485 c, t dbSNP:773579272
493 493 g, t dbSNP:765571435
494 494 g, t dbSNP:762162139
506 506 c, t dbSNP:776685788
508 508 c, t dbSNP:768830740
510 510 -, tt dbSNP:779449960
513 513 c, t dbSNP:11465209
518 518 a, c dbSNP:775673556
520 520 a, c dbSNP:770998731
524 524 a, c dbSNP:749183591
527 527 c, t dbSNP:777772044
529 529 c, g dbSNP:199968990
530 530 c, g dbSNP:747879087
539 539 c, g dbSNP:780727289
546 546 c, t dbSNP:754615206
558 558 c, t dbSNP:1611772
559 559 a, g dbSNP:549073561
561 561 c, t dbSNP:369597551
562 562 a, g dbSNP:139043100
564 564 a, c dbSNP:765630606
565 565 c, t dbSNP:762060905
574 574 c, g dbSNP:777006162
578 578 a, g dbSNP:764254987
579 579 c, g, t dbSNP:775726708
592 592 a, g dbSNP:772268236
600 600 c, t dbSNP:752708990
602 602 a, g dbSNP:773256715
608 608 g, t dbSNP:769683074
631 631 c, t dbSNP:1611773
677 677 a, g dbSNP:779032625
706 706 c, t dbSNP:780969195
707 707 a, g dbSNP:756031439
720 720 c, t dbSNP:768203896
722 722 c, t dbSNP:746647874
749 749 a, g dbSNP:11465210
769 769 g, t dbSNP:750380223
784 784 a, t dbSNP:188749315
796 796 c, t dbSNP:757872424
803 803 a, g dbSNP:750976032
818 818 a, g, t dbSNP:112893627
831 831 a, g dbSNP:767317412
859 859 a, g dbSNP:533295118

Target ORF information:

RefSeq Version NM_001044392
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001044392

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTTA ATTCCTCTCT GGAAGATCCC AGCACCGACT ACTACCAAGA GCTGCAGAGA
GACATTTCTG AAATGGCTGT CTGTCAGTGC CGCCGAAAGA ACTACGGGCA GCTGGACATC
TTTCCAGCCC GGGATACCTA CCATCCTATG AGCGAGTACC CCACCTACCA CACCCATGGG
CGCTATGTGC CCCCTAGCAG TACCGATCGT AGCCCCTATG AGAAGGTTTC TGCAGGTAAT
GGTGGCAGCA GCCTCTCTTA CACAAACCCA GCAGTGGCAG CCACTTCTGC CAACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22660
Accession Version NM_001044390.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 612bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 5 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, AY327582.1, AC234582.3 and AA425062.1. On Feb 26, 2011 this sequence version replaced gi:113206023. Summary: This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]. Transcript Variant: This variant (5) has multiple differences which result in a frameshift in the central coding region, compared to variant 1. These differences result in a shorter isoform (5), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY327582.1, AY327591.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154405 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTA TCCCAGCACC GACTACTACC
AAGAGCTGCA GAGAGACATT TCTGAAATGT TTTTGCAGAT TTATAAACAA GGGGGTTTTC
TGGGCCTCTC CAATATTAAG TTCAGTGAGT GATGTGCCAT TTCCTTTCTC TGCCCAGTCT
GGGGCTGGGG TGCCAGGCTG GGGCATCGCG CTGCTGGTGC TGGTCTGTGT TCTGGTTGCG
CTGGCCATTG TCTATCTCAT TGCCTTGGCT GTCTGTCAGT GCCGCCGAAA GAACTACGGG
CAGCTGGACA TCTTTCCAGC CCGGGATACC TACCATCCTA TGAGCGAGTA CCCCACCTAC
CACACCCATG GGCGCTATGT GCCCCCTAGC AGTACCGATC GTAGCCCCTA TGAGAAGGTT
TCTGCAGGTA ATGGTGGCAG CAGCCTCTCT TACACAAACC CAGCAGTGGC AGCCACTTCT
GCCAACTTGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001037855.1
CDS73..684
Misc Feature(1)73..141(+)
Misc Feature(2)487..489(+)
Misc Feature(3)523..525(+)
Misc Feature(4)541..543(+)
Misc Feature(5)550..552(+)
Misc Feature(6)559..>666(+)
Misc Feature(7)601..603(+)
Misc Feature(8)643..645(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..231
Gene:MUC1
Gene Synonym:
Exon (3)232..281
Gene:MUC1
Gene Synonym:
Exon (4)282..337
Gene:MUC1
Gene Synonym:
Exon (5)338..459
Gene:MUC1
Gene Synonym:
Exon (6)460..609
Gene:MUC1
Gene Synonym:
Exon (7)610..995
Gene:MUC1
Gene Synonym:
Translation
Position Chain Variation Link
1 1 c, t dbSNP:562400415
2 2 a, g dbSNP:11465204
20 20 c, t dbSNP:372118515
24 24 c, t dbSNP:368256181
25 25 a, g, t dbSNP:764293615
26 26 c, t dbSNP:760667690
32 32 g, t dbSNP:774386878
33 33 c, g dbSNP:770752618
35 35 a, c dbSNP:749183212
39 39 a, g dbSNP:772996200
41 41 -, t dbSNP:753898556
46 46 c, t dbSNP:769518985
48 48 c, t dbSNP:564315578
65 65 c, t dbSNP:747855021
77 77 c, t dbSNP:780700800
80 80 c, t dbSNP:376608767
81 81 a, g dbSNP:373505102
87 87 c, t dbSNP:779571432
92 92 c, g dbSNP:368800613
100 100 c, t dbSNP:759009730
103 103 c, t dbSNP:377356836
137 137 c, t dbSNP:139437006
138 138 a, g dbSNP:4072037
140 140 a, g dbSNP:766333007
145 145 a, g dbSNP:773637715
155 155 g, t dbSNP:145108039
158 158 c, t dbSNP:145667707
160 160 a, t dbSNP:776982023
163 163 a, c dbSNP:575065522
169 169 a, g dbSNP:771925604
170 170 a, g dbSNP:201761107
173 173 a, g dbSNP:758452127
176 176 a, g dbSNP:745939858
182 182 c, t dbSNP:201186912
183 183 g, t dbSNP:544640619
185 185 c, g, t dbSNP:577380147
186 186 a, c, g dbSNP:751297222
191 191 a, c dbSNP:766031544
192 192 c, g dbSNP:199568358
195 195 a, g dbSNP:749976676
205 205 g, t dbSNP:764582269
210 210 c, t dbSNP:762318074
213 213 a, c, t dbSNP:145691584
215 215 a, c dbSNP:761000946
216 216 c, t dbSNP:200478078
231 231 c, t dbSNP:772361769
234 234 c, t dbSNP:146950322
239 239 -, c dbSNP:774368817
243 243 a, g dbSNP:765746637
247 247 a, g dbSNP:763569821
252 252 c, g dbSNP:773704188
255 255 c, g dbSNP:770183823
293 293 g, t dbSNP:745924162
305 305 a, g dbSNP:140871200
325 325 a, g dbSNP:780669552
326 326 c, t dbSNP:370819310
328 328 g, t dbSNP:781095660
341 341 a, g dbSNP:11465207
351 351 a, t dbSNP:752680798
371 371 c, g dbSNP:199840128
374 374 a, g dbSNP:369649894
380 380 a, g dbSNP:369608212
381 381 a, g dbSNP:774139080
395 395 -, g dbSNP:761859668
397 397 a, g dbSNP:766143764
399 399 c, t dbSNP:375090196
400 400 a, g, t dbSNP:373081136
401 401 c, t dbSNP:747711729
402 402 a, g dbSNP:776240199
403 403 a, c dbSNP:769289843
409 409 a, g, t dbSNP:780622331
414 414 a, g dbSNP:758786611
415 415 c, g dbSNP:746119110
420 420 c, t dbSNP:779173931
421 421 a, g dbSNP:757373880
431 431 c, t dbSNP:200788986
432 432 a, g dbSNP:764097944
435 435 g, t dbSNP:141460657
439 439 a, g dbSNP:751488017
442 442 a, g dbSNP:766124835
446 446 a, g dbSNP:183700327
447 447 c, t dbSNP:772830298
458 458 c, t dbSNP:199768496
466 466 -, tgtc dbSNP:747704526
476 476 a, g dbSNP:764882391
478 478 c, t dbSNP:761613959
482 482 -, a dbSNP:780480243
484 484 a, g dbSNP:753535070
486 486 c, g dbSNP:763738651
489 489 c, t dbSNP:773917931
490 490 a, g dbSNP:774814129
502 502 a, g dbSNP:772667594
512 512 c, g dbSNP:557552446
514 514 c, t dbSNP:774796958
515 515 a, g dbSNP:771275191
517 517 a, g dbSNP:749581526
535 535 a, c dbSNP:777946901
537 537 c, g, t dbSNP:200639498
538 538 a, g dbSNP:768456378
542 542 a, c dbSNP:781066358
547 547 a, c dbSNP:758368648
550 550 g, t dbSNP:750312588
559 559 c, t dbSNP:778833490
560 560 a, t dbSNP:757088101
565 565 c, t dbSNP:572153326
566 566 a, g dbSNP:763861742
584 584 a, g dbSNP:760364466
588 588 a, c, t dbSNP:766884618
589 589 a, g dbSNP:760149442
592 592 c, g, t dbSNP:372682991
593 593 g, t dbSNP:191544901
602 602 a, g dbSNP:148332231
617 617 c, t dbSNP:773579272
625 625 g, t dbSNP:765571435
626 626 g, t dbSNP:762162139
638 638 c, t dbSNP:776685788
640 640 c, t dbSNP:768830740
642 642 -, tt dbSNP:779449960
645 645 c, t dbSNP:11465209
650 650 a, c dbSNP:775673556
652 652 a, c dbSNP:770998731
656 656 a, c dbSNP:749183591
659 659 c, t dbSNP:777772044
661 661 c, g dbSNP:199968990
662 662 c, g dbSNP:747879087
671 671 c, g dbSNP:780727289
678 678 c, t dbSNP:754615206
690 690 c, t dbSNP:1611772
691 691 a, g dbSNP:549073561
693 693 c, t dbSNP:369597551
694 694 a, g dbSNP:139043100
696 696 a, c dbSNP:765630606
697 697 c, t dbSNP:762060905
706 706 c, g dbSNP:777006162
710 710 a, g dbSNP:764254987
711 711 c, g, t dbSNP:775726708
724 724 a, g dbSNP:772268236
732 732 c, t dbSNP:752708990
734 734 a, g dbSNP:773256715
740 740 g, t dbSNP:769683074
763 763 c, t dbSNP:1611773
809 809 a, g dbSNP:779032625
838 838 c, t dbSNP:780969195
839 839 a, g dbSNP:756031439
852 852 c, t dbSNP:768203896
854 854 c, t dbSNP:746647874
881 881 a, g dbSNP:11465210
901 901 g, t dbSNP:750380223
916 916 a, t dbSNP:188749315
928 928 c, t dbSNP:757872424
935 935 a, g dbSNP:750976032
950 950 a, g, t dbSNP:112893627
963 963 a, g dbSNP:767317412
991 991 a, g dbSNP:533295118

Target ORF information:

RefSeq Version NM_001044390
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001044390

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTA TCCCAGCACC GACTACTACC
AAGAGCTGCA GAGAGACATT TCTGAAATGT TTTTGCAGAT TTATAAACAA GGGGGTTTTC
TGGGCCTCTC CAATATTAAG TTCAGTGAGT GATGTGCCAT TTCCTTTCTC TGCCCAGTCT
GGGGCTGGGG TGCCAGGCTG GGGCATCGCG CTGCTGGTGC TGGTCTGTGT TCTGGTTGCG
CTGGCCATTG TCTATCTCAT TGCCTTGGCT GTCTGTCAGT GCCGCCGAAA GAACTACGGG
CAGCTGGACA TCTTTCCAGC CCGGGATACC TACCATCCTA TGAGCGAGTA CCCCACCTAC
CACACCCATG GGCGCTATGT GCCCCCTAGC AGTACCGATC GTAGCCCCTA TGAGAAGGTT
TCTGCAGGTA ATGGTGGCAG CAGCCTCTCT TACACAAACC CAGCAGTGGC AGCCACTTCT
GCCAACTTGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22896
Accession Version NM_001018017.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 768bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, BC120974.2, AC234582.3 and AA425062.1. On Feb 26, 2011 this sequence version replaced gi:67189068. Summary: This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]. Transcript Variant: This variant (3) uses an alternate in-frame splice site, compared to variant 1. The resulting isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC120974.2, U60260.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTT TTAATTCCTC TCTGGAAGAT
CCCAGCACCG ACTACTACCA AGAGCTGCAG AGAGACATTT CTGAAATGTT TTTGCAGATT
TATAAACAAG GGGGTTTTCT GGGCCTCTCC AATATTAAGT TCAGGCCAGG ATCTGTGGTG
GTACAATTGA CTCTGGCCTT CCGAGAAGGT ACCATCAATG TCCACGACGT GGAGACACAG
TTCAATCAGT ATAAAACGGA AGCAGCCTCT CGATATAACC TGACGATCTC AGACGTCAGC
GTGAGTGATG TGCCATTTCC TTTCTCTGCC CAGTCTGGGG CTGGGGTGCC AGGCTGGGGC
ATCGCGCTGC TGGTGCTGGT CTGTGTTCTG GTTGCGCTGG CCATTGTCTA TCTCATTGCC
TTGGCTGTCT GTCAGTGCCG CCGAAAGAAC TACGGGCAGC TGGACATCTT TCCAGCCCGG
GATACCTACC ATCCTATGAG CGAGTACCCC ACCTACCACA CCCATGGGCG CTATGTGCCC
CCTAGCAGTA CCGATCGTAG CCCCTATGAG AAGGTTTCTG CAGGTAATGG TGGCAGCAGC
CTCTCTTACA CAAACCCAGC AGTGGCAGCC ACTTCTGCCA ACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001018017.1
CDS73..840
Misc Feature(1)73..141(+)
Misc Feature(2)151..153(+)
Misc Feature(3)178..528(+)
Misc Feature(4)343..345(+)
Misc Feature(5)361..363(+)
Misc Feature(6)397..399(+)
Misc Feature(7)469..471(+)
Misc Feature(8)643..645(+)
Misc Feature(9)679..681(+)
Misc Feature(10)697..699(+)
Misc Feature(11)706..708(+)
Misc Feature(12)742..744(+)
Misc Feature(13)751..753(+)
Misc Feature(14)757..759(+)
Misc Feature(15)757..759(+)
Misc Feature(16)799..801(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..231
Gene:MUC1
Gene Synonym:
Exon (3)232..300
Gene:MUC1
Gene Synonym:
Exon (4)301..356
Gene:MUC1
Gene Synonym:
Exon (5)357..493
Gene:MUC1
Gene Synonym:
Exon (6)494..615
Gene:MUC1
Gene Synonym:
Exon (7)616..765
Gene:MUC1
Gene Synonym:
Exon (8)766..1151
Gene:MUC1
Gene Synonym:
Translation
Position Chain Variation Link
1 1 c, t dbSNP:562400415
2 2 a, g dbSNP:11465204
20 20 c, t dbSNP:372118515
24 24 c, t dbSNP:368256181
25 25 a, g, t dbSNP:764293615
26 26 c, t dbSNP:760667690
32 32 g, t dbSNP:774386878
33 33 c, g dbSNP:770752618
35 35 a, c dbSNP:749183212
39 39 a, g dbSNP:772996200
41 41 -, t dbSNP:753898556
46 46 c, t dbSNP:769518985
48 48 c, t dbSNP:564315578
65 65 c, t dbSNP:747855021
77 77 c, t dbSNP:780700800
80 80 c, t dbSNP:376608767
81 81 a, g dbSNP:373505102
87 87 c, t dbSNP:779571432
92 92 c, g dbSNP:368800613
100 100 c, t dbSNP:759009730
103 103 c, t dbSNP:377356836
137 137 c, t dbSNP:139437006
138 138 a, g dbSNP:4072037
140 140 a, g dbSNP:766333007
145 145 a, g dbSNP:773637715
155 155 g, t dbSNP:145108039
158 158 c, t dbSNP:145667707
160 160 a, t dbSNP:776982023
163 163 a, c dbSNP:575065522
169 169 a, g dbSNP:771925604
170 170 a, g dbSNP:201761107
173 173 a, g dbSNP:758452127
176 176 a, g dbSNP:745939858
182 182 c, t dbSNP:201186912
183 183 g, t dbSNP:544640619
185 185 c, g, t dbSNP:577380147
186 186 a, c, g dbSNP:751297222
191 191 a, c dbSNP:766031544
192 192 c, g dbSNP:199568358
195 195 a, g dbSNP:749976676
205 205 g, t dbSNP:764582269
210 210 c, t dbSNP:762318074
213 213 a, c, t dbSNP:145691584
215 215 a, c dbSNP:761000946
216 216 c, t dbSNP:200478078
231 231 c, t dbSNP:772361769
241 241 a, t dbSNP:35819649
243 243 c, t dbSNP:767259710
244 244 c, t dbSNP:759068582
246 246 c, g dbSNP:751081819
253 253 c, t dbSNP:146950322
258 258 -, c dbSNP:774368817
262 262 a, g dbSNP:765746637
266 266 a, g dbSNP:763569821
271 271 c, g dbSNP:773704188
274 274 c, g dbSNP:770183823
312 312 g, t dbSNP:745924162
324 324 a, g dbSNP:140871200
344 344 a, g dbSNP:780669552
345 345 c, t dbSNP:370819310
347 347 g, t dbSNP:781095660
357 357 a, g dbSNP:768544711
359 359 -, c dbSNP:777127574
362 362 g, t dbSNP:145224844
364 364 a, t dbSNP:373382247
378 378 a, g dbSNP:139620330
383 383 c, t dbSNP:745467701
393 393 c, t dbSNP:778454369
395 395 g, t dbSNP:756756401
399 399 a, g dbSNP:370391810
401 401 c, g dbSNP:758430992
408 408 c, t dbSNP:764490455
413 413 a, t dbSNP:532835613
418 418 a, g, t dbSNP:144273480
420 420 c, t dbSNP:759892292
421 421 a, g, t dbSNP:1611770
437 437 a, g dbSNP:200411609
438 438 a, t dbSNP:41264913
443 443 a, g dbSNP:773293207
445 445 a, g dbSNP:768505970
449 449 c, g, t dbSNP:775163504
450 450 a, g dbSNP:561379331
453 453 a, g dbSNP:745587505
458 458 a, c dbSNP:766169396
463 463 a, c dbSNP:778505736
464 464 g, t dbSNP:770482054
468 468 c, t dbSNP:748774119
476 476 c, t dbSNP:146141676
477 477 a, g dbSNP:201798179
486 486 a, c, t dbSNP:781731301
487 487 a, g dbSNP:755359583
489 489 c, t dbSNP:751875756
492 492 c, t dbSNP:766729514
493 493 a, g dbSNP:201815857
497 497 a, g dbSNP:11465207
507 507 a, t dbSNP:752680798
527 527 c, g dbSNP:199840128
530 530 a, g dbSNP:369649894
536 536 a, g dbSNP:369608212
537 537 a, g dbSNP:774139080
551 551 -, g dbSNP:761859668
553 553 a, g dbSNP:766143764
555 555 c, t dbSNP:375090196
556 556 a, g, t dbSNP:373081136
557 557 c, t dbSNP:747711729
558 558 a, g dbSNP:776240199
559 559 a, c dbSNP:769289843
565 565 a, g, t dbSNP:780622331
570 570 a, g dbSNP:758786611
571 571 c, g dbSNP:746119110
576 576 c, t dbSNP:779173931
577 577 a, g dbSNP:757373880
587 587 c, t dbSNP:200788986
588 588 a, g dbSNP:764097944
591 591 g, t dbSNP:141460657
595 595 a, g dbSNP:751488017
598 598 a, g dbSNP:766124835
602 602 a, g dbSNP:183700327
603 603 c, t dbSNP:772830298
614 614 c, t dbSNP:199768496
622 622 -, tgtc dbSNP:747704526
632 632 a, g dbSNP:764882391
634 634 c, t dbSNP:761613959
638 638 -, a dbSNP:780480243
640 640 a, g dbSNP:753535070
642 642 c, g dbSNP:763738651
645 645 c, t dbSNP:773917931
646 646 a, g dbSNP:774814129
658 658 a, g dbSNP:772667594
668 668 c, g dbSNP:557552446
670 670 c, t dbSNP:774796958
671 671 a, g dbSNP:771275191
673 673 a, g dbSNP:749581526
691 691 a, c dbSNP:777946901
693 693 c, g, t dbSNP:200639498
694 694 a, g dbSNP:768456378
698 698 a, c dbSNP:781066358
703 703 a, c dbSNP:758368648
706 706 g, t dbSNP:750312588
715 715 c, t dbSNP:778833490
716 716 a, t dbSNP:757088101
721 721 c, t dbSNP:572153326
722 722 a, g dbSNP:763861742
740 740 a, g dbSNP:760364466
744 744 a, c, t dbSNP:766884618
745 745 a, g dbSNP:760149442
748 748 c, g, t dbSNP:372682991
749 749 g, t dbSNP:191544901
758 758 a, g dbSNP:148332231
773 773 c, t dbSNP:773579272
781 781 g, t dbSNP:765571435
782 782 g, t dbSNP:762162139
794 794 c, t dbSNP:776685788
796 796 c, t dbSNP:768830740
798 798 -, tt dbSNP:779449960
801 801 c, t dbSNP:11465209
806 806 a, c dbSNP:775673556
808 808 a, c dbSNP:770998731
812 812 a, c dbSNP:749183591
815 815 c, t dbSNP:777772044
817 817 c, g dbSNP:199968990
818 818 c, g dbSNP:747879087
827 827 c, g dbSNP:780727289
834 834 c, t dbSNP:754615206
846 846 c, t dbSNP:1611772
847 847 a, g dbSNP:549073561
849 849 c, t dbSNP:369597551
850 850 a, g dbSNP:139043100
852 852 a, c dbSNP:765630606
853 853 c, t dbSNP:762060905
862 862 c, g dbSNP:777006162
866 866 a, g dbSNP:764254987
867 867 c, g, t dbSNP:775726708
880 880 a, g dbSNP:772268236
888 888 c, t dbSNP:752708990
890 890 a, g dbSNP:773256715
896 896 g, t dbSNP:769683074
919 919 c, t dbSNP:1611773
965 965 a, g dbSNP:779032625
994 994 c, t dbSNP:780969195
995 995 a, g dbSNP:756031439
1008 1008 c, t dbSNP:768203896
1010 1010 c, t dbSNP:746647874
1037 1037 a, g dbSNP:11465210
1057 1057 g, t dbSNP:750380223
1072 1072 a, t dbSNP:188749315
1084 1084 c, t dbSNP:757872424
1091 1091 a, g dbSNP:750976032
1106 1106 a, g, t dbSNP:112893627
1119 1119 a, g dbSNP:767317412
1147 1147 a, g dbSNP:533295118

Target ORF information:

RefSeq Version NM_001018017
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001018017

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTT TTAATTCCTC TCTGGAAGAT
CCCAGCACCG ACTACTACCA AGAGCTGCAG AGAGACATTT CTGAAATGTT TTTGCAGATT
TATAAACAAG GGGGTTTTCT GGGCCTCTCC AATATTAAGT TCAGGCCAGG ATCTGTGGTG
GTACAATTGA CTCTGGCCTT CCGAGAAGGT ACCATCAATG TCCACGACGT GGAGACACAG
TTCAATCAGT ATAAAACGGA AGCAGCCTCT CGATATAACC TGACGATCTC AGACGTCAGC
GTGAGTGATG TGCCATTTCC TTTCTCTGCC CAGTCTGGGG CTGGGGTGCC AGGCTGGGGC
ATCGCGCTGC TGGTGCTGGT CTGTGTTCTG GTTGCGCTGG CCATTGTCTA TCTCATTGCC
TTGGCTGTCT GTCAGTGCCG CCGAAAGAAC TACGGGCAGC TGGACATCTT TCCAGCCCGG
GATACCTACC ATCCTATGAG CGAGTACCCC ACCTACCACA CCCATGGGCG CTATGTGCCC
CCTAGCAGTA CCGATCGTAG CCCCTATGAG AAGGTTTCTG CAGGTAATGG TGGCAGCAGC
CTCTCTTACA CAAACCCAGC AGTGGCAGCC ACTTCTGCCA ACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23198
Accession Version NM_001204285.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1428bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 9 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, AC234582.3 and AA425062.1. Summary: This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]. Transcript Variant: This variant (9) has multiple differences but maintains the reading frame, compared to variant 1. The encoded isoform (9) is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J05581.1, AH001452.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2154405 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTG TGAGTATGAC CAGCAGCGTA
CTCTCCAGCC ACAGCCCCGG TTCAGGCTCC TCCACCACTC AGGGACAGGA TGTCACTCTG
GCCCCGGCCA CGGAACCAGC TTCAGGTTCA GCTGCCACCT GGGGACAGGA TGTCACCTCG
GTCCCAGTCA CCAGGCCAGC CCTGGGCTCC ACCACCCCGC CAGCCCACGA TGTCACCTCA
GCCCCGGACA ACAAGCCAGC CCCGGGCTCC ACCGCCCCCC CAGCCCACGG TGTCACCTCG
GCCCCGGACA CCAGGCCGGC CCCGGGCTCC ACCGCCCCCC CAGCCCATGG TGTCACCTCG
GCCCCGGACA ACAGGCCCGC CTTGGGCTCC ACCGCCCCTC CAGTCCACAA TGTCACCTCG
GCCTCAGGCT CTGCATCAGG CTCAGCTTCT ACTCTGGTGC ACAACGGCAC CTCTGCCAGG
GCTACCACAA CCCCAGCCAG CAAGAGCACT CCATTCTCAA TTCCCAGCCA CCACTCTGAT
ACTCCTACCA CCCTTGCCAG CCATAGCACC AAGACTGATG CCAGTAGCAC TCACCATAGC
ACGGTACCTC CTCTCACCTC CTCCAATCAC AGCACTTCTC CCCAGTTGTC TACTGGGGTC
TCTTTCTTTT TCCTGTCTTT TCACATTTCA AACCTCCAGT TTAATTCCTC TCTGGAAGAT
CCCAGCACCG ACTACTACCA AGAGCTGCAG AGAGACATTT CTGAAATGTT TTTGCAGATT
TATAAACAAG GGGGTTTTCT GGGCCTCTCC AATATTAAGT TCAGGCCAGG ATCTGTGGTG
GTACAATTGA CTCTGGCCTT CCGAGAAGGT ACCATCAATG TCCACGACGT GGAGACACAG
TTCAATCAGT ATAAAACGGA AGCAGCCTCT CGATATAACC TGACGATCTC AGACGTCAGC
GTGAGTGATG TGCCATTTCC TTTCTCTGCC CAGTCTGGGG CTGGGGTGCC AGGCTGGGGC
ATCGCGCTGC TGGTGCTGGT CTGTGTTCTG GTTGCGCTGG CCATTGTCTA TCTCATTGCC
TTGGCTGTCT GTCAGTGCCG CCGAAAGAAC TACGGGCAGC TGGACATCTT TCCAGCCCGG
GATACCTACC ATCCTATGAG CGAGTACCCC ACCTACCACA CCCATGGGCG CTATGTGCCC
CCTAGCAGTA CCGATCGTAG CCCCTATGAG AAGGTTTCTG CAGGTAATGG TGGCAGCAGC
CTCTCTTACA CAAACCCAGC AGTGGCAGCC ACTTCTGCCA ACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001191214.1
CDS73..1500
Misc Feature(1)73..141(+)
Misc Feature(2)352..>561(+)
Misc Feature(3)832..1188(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..506
Gene:MUC1
Gene Synonym:
Exon (3)507..960
Gene:MUC1
Gene Synonym:
Exon (4)961..1016
Gene:MUC1
Gene Synonym:
Exon (5)1017..1153
Gene:MUC1
Gene Synonym:
Exon (6)1154..1275
Gene:MUC1
Gene Synonym:
Exon (7)1276..1425
Gene:MUC1
Gene Synonym:
Exon (8)1426..1811
Gene:MUC1
Gene Synonym:
Translation
Position Chain Variation Link
1 1 c, t dbSNP:562400415
2 2 a, g dbSNP:11465204
20 20 c, t dbSNP:372118515
24 24 c, t dbSNP:368256181
25 25 a, g, t dbSNP:764293615
26 26 c, t dbSNP:760667690
32 32 g, t dbSNP:774386878
33 33 c, g dbSNP:770752618
35 35 a, c dbSNP:749183212
39 39 a, g dbSNP:772996200
41 41 -, t dbSNP:753898556
46 46 c, t dbSNP:769518985
48 48 c, t dbSNP:564315578
65 65 c, t dbSNP:747855021
77 77 c, t dbSNP:780700800
80 80 c, t dbSNP:376608767
81 81 a, g dbSNP:373505102
87 87 c, t dbSNP:779571432
92 92 c, g dbSNP:368800613
100 100 c, t dbSNP:759009730
103 103 c, t dbSNP:377356836
137 137 c, t dbSNP:139437006
138 138 a, g dbSNP:4072037
140 140 a, g dbSNP:766333007
145 145 a, g dbSNP:773637715
155 155 g, t dbSNP:145108039
158 158 c, t dbSNP:145667707
160 160 a, t dbSNP:776982023
163 163 a, c dbSNP:575065522
169 169 a, g dbSNP:771925604
170 170 a, g dbSNP:201761107
173 173 a, g dbSNP:758452127
176 176 a, g dbSNP:745939858
182 182 c, t dbSNP:201186912
183 183 g, t dbSNP:544640619
185 185 c, g, t dbSNP:577380147
186 186 a, c, g dbSNP:751297222
191 191 a, c dbSNP:766031544
192 192 c, g dbSNP:199568358
195 195 a, g dbSNP:749976676
205 205 g, t dbSNP:764582269
210 210 c, t dbSNP:762318074
213 213 a, c, t dbSNP:145691584
215 215 a, c dbSNP:761000946
216 216 c, t dbSNP:200478078
231 231 c, t dbSNP:772361769
236 236 a, g dbSNP:746039202
245 245 a, g dbSNP:779056324
250 250 a, g dbSNP:573074227
256 256 c, t dbSNP:748161372
257 257 c, t dbSNP:781244387
260 260 a, g dbSNP:369747755
264 264 c, g dbSNP:375294990
270 270 c, t dbSNP:554510794
271 271 a, g dbSNP:149173724
272 272 a, g dbSNP:749982120
280 280 c, t dbSNP:764862472
281 281 a, c dbSNP:761311662
284 284 a, c dbSNP:754388111
285 285 c, t dbSNP:764566457
287 287 a, c, t dbSNP:775954658
288 288 a, c dbSNP:772260996
292 292 c, g dbSNP:759820018
296 296 a, g dbSNP:774430804
316 316 c, t dbSNP:771172793
317 317 c, t dbSNP:749356163
318 318 a, c, g dbSNP:569077024
319 319 a, g dbSNP:746850676
323 323 c, t dbSNP:779909381
324 324 a, g dbSNP:550513633
327 327 a, g dbSNP:577679407
329 329 a, c dbSNP:778509723
345 345 g, t dbSNP:538255129
351 351 c, t dbSNP:753369817
356 356 g, t dbSNP:570961786
358 358 c, t dbSNP:756650252
657 657 c, t dbSNP:542758718
658 658 a, g, t dbSNP:11465205
661 661 a, t dbSNP:746313723
665 665 a, c dbSNP:779160336
674 674 c, g dbSNP:779962710
677 677 c, g dbSNP:749541505
689 689 c, t dbSNP:556778160
690 690 c, g, t dbSNP:752803449
694 694 a, c dbSNP:767518733
696 696 a, g dbSNP:758344731
698 698 a, g dbSNP:750357078
709 709 c, t dbSNP:765005265
718 718 a, g dbSNP:761635265
719 719 c, g dbSNP:776192852
720 720 c, g, t dbSNP:202108781
733 733 a, t dbSNP:760366647
735 735 c, t dbSNP:775147912
736 736 c, g dbSNP:771654907
740 740 c, t dbSNP:746421166
743 743 a, c dbSNP:774919577
748 748 g, t dbSNP:771241325
750 750 c, t dbSNP:749683215
753 753 a, c dbSNP:778070113
767 767 a, c, t dbSNP:748311438
786 786 c, g dbSNP:149945265
794 794 c, t dbSNP:529196978
795 795 a, g dbSNP:750365491
803 803 c, t dbSNP:765196106
811 811 g, t dbSNP:757095335
812 812 c, g dbSNP:201308716
813 813 c, t dbSNP:763918894
814 814 c, t dbSNP:760421618
818 818 a, g dbSNP:775268792
830 830 c, g dbSNP:767204590
832 832 a, c dbSNP:759057639
837 837 g, t dbSNP:563563693
840 840 g, t dbSNP:771498973
844 844 a, c dbSNP:749690550
850 850 a, g, t dbSNP:770126594
853 853 c, t dbSNP:748436056
854 854 c, t dbSNP:781359601
863 863 g, t dbSNP:768665660
864 864 c, t dbSNP:747132214
869 869 c, t dbSNP:534337862
876 876 c, t dbSNP:371485486
885 885 c, t dbSNP:753660394
891 891 a, c, g dbSNP:755973313
901 901 a, t dbSNP:35819649
903 903 c, t dbSNP:767259710
904 904 c, t dbSNP:759068582
906 906 c, g dbSNP:751081819
913 913 c, t dbSNP:146950322
918 918 -, c dbSNP:774368817
922 922 a, g dbSNP:765746637
926 926 a, g dbSNP:763569821
931 931 c, g dbSNP:773704188
934 934 c, g dbSNP:770183823
972 972 g, t dbSNP:745924162
984 984 a, g dbSNP:140871200
1004 1004 a, g dbSNP:780669552
1005 1005 c, t dbSNP:370819310
1007 1007 g, t dbSNP:781095660
1017 1017 a, g dbSNP:768544711
1019 1019 -, c dbSNP:777127574
1022 1022 g, t dbSNP:145224844
1024 1024 a, t dbSNP:373382247
1038 1038 a, g dbSNP:139620330
1043 1043 c, t dbSNP:745467701
1053 1053 c, t dbSNP:778454369
1055 1055 g, t dbSNP:756756401
1059 1059 a, g dbSNP:370391810
1061 1061 c, g dbSNP:758430992
1068 1068 c, t dbSNP:764490455
1073 1073 a, t dbSNP:532835613
1078 1078 a, g, t dbSNP:144273480
1080 1080 c, t dbSNP:759892292
1081 1081 a, g, t dbSNP:1611770
1097 1097 a, g dbSNP:200411609
1098 1098 a, t dbSNP:41264913
1103 1103 a, g dbSNP:773293207
1105 1105 a, g dbSNP:768505970
1109 1109 c, g, t dbSNP:775163504
1110 1110 a, g dbSNP:561379331
1113 1113 a, g dbSNP:745587505
1118 1118 a, c dbSNP:766169396
1123 1123 a, c dbSNP:778505736
1124 1124 g, t dbSNP:770482054
1128 1128 c, t dbSNP:748774119
1136 1136 c, t dbSNP:146141676
1137 1137 a, g dbSNP:201798179
1146 1146 a, c, t dbSNP:781731301
1147 1147 a, g dbSNP:755359583
1149 1149 c, t dbSNP:751875756
1152 1152 c, t dbSNP:766729514
1153 1153 a, g dbSNP:201815857
1157 1157 a, g dbSNP:11465207
1167 1167 a, t dbSNP:752680798
1187 1187 c, g dbSNP:199840128
1190 1190 a, g dbSNP:369649894
1196 1196 a, g dbSNP:369608212
1197 1197 a, g dbSNP:774139080
1211 1211 -, g dbSNP:761859668
1213 1213 a, g dbSNP:766143764
1215 1215 c, t dbSNP:375090196
1216 1216 a, g, t dbSNP:373081136
1217 1217 c, t dbSNP:747711729
1218 1218 a, g dbSNP:776240199
1219 1219 a, c dbSNP:769289843
1225 1225 a, g, t dbSNP:780622331
1230 1230 a, g dbSNP:758786611
1231 1231 c, g dbSNP:746119110
1236 1236 c, t dbSNP:779173931
1237 1237 a, g dbSNP:757373880
1247 1247 c, t dbSNP:200788986
1248 1248 a, g dbSNP:764097944
1251 1251 g, t dbSNP:141460657
1255 1255 a, g dbSNP:751488017
1258 1258 a, g dbSNP:766124835
1262 1262 a, g dbSNP:183700327
1263 1263 c, t dbSNP:772830298
1274 1274 c, t dbSNP:199768496
1282 1282 -, tgtc dbSNP:747704526
1292 1292 a, g dbSNP:764882391
1294 1294 c, t dbSNP:761613959
1298 1298 -, a dbSNP:780480243
1300 1300 a, g dbSNP:753535070
1302 1302 c, g dbSNP:763738651
1305 1305 c, t dbSNP:773917931
1306 1306 a, g dbSNP:774814129
1318 1318 a, g dbSNP:772667594
1328 1328 c, g dbSNP:557552446
1330 1330 c, t dbSNP:774796958
1331 1331 a, g dbSNP:771275191
1333 1333 a, g dbSNP:749581526
1351 1351 a, c dbSNP:777946901
1353 1353 c, g, t dbSNP:200639498
1354 1354 a, g dbSNP:768456378
1358 1358 a, c dbSNP:781066358
1363 1363 a, c dbSNP:758368648
1366 1366 g, t dbSNP:750312588
1375 1375 c, t dbSNP:778833490
1376 1376 a, t dbSNP:757088101
1381 1381 c, t dbSNP:572153326
1382 1382 a, g dbSNP:763861742
1400 1400 a, g dbSNP:760364466
1404 1404 a, c, t dbSNP:766884618
1405 1405 a, g dbSNP:760149442
1408 1408 c, g, t dbSNP:372682991
1409 1409 g, t dbSNP:191544901
1418 1418 a, g dbSNP:148332231
1433 1433 c, t dbSNP:773579272
1441 1441 g, t dbSNP:765571435
1442 1442 g, t dbSNP:762162139
1454 1454 c, t dbSNP:776685788
1456 1456 c, t dbSNP:768830740
1458 1458 -, tt dbSNP:779449960
1461 1461 c, t dbSNP:11465209
1466 1466 a, c dbSNP:775673556
1468 1468 a, c dbSNP:770998731
1472 1472 a, c dbSNP:749183591
1475 1475 c, t dbSNP:777772044
1477 1477 c, g dbSNP:199968990
1478 1478 c, g dbSNP:747879087
1487 1487 c, g dbSNP:780727289
1494 1494 c, t dbSNP:754615206
1506 1506 c, t dbSNP:1611772
1507 1507 a, g dbSNP:549073561
1509 1509 c, t dbSNP:369597551
1510 1510 a, g dbSNP:139043100
1512 1512 a, c dbSNP:765630606
1513 1513 c, t dbSNP:762060905
1522 1522 c, g dbSNP:777006162
1526 1526 a, g dbSNP:764254987
1527 1527 c, g, t dbSNP:775726708
1540 1540 a, g dbSNP:772268236
1548 1548 c, t dbSNP:752708990
1550 1550 a, g dbSNP:773256715
1556 1556 g, t dbSNP:769683074
1579 1579 c, t dbSNP:1611773
1625 1625 a, g dbSNP:779032625
1654 1654 c, t dbSNP:780969195
1655 1655 a, g dbSNP:756031439
1668 1668 c, t dbSNP:768203896
1670 1670 c, t dbSNP:746647874
1697 1697 a, g dbSNP:11465210
1717 1717 g, t dbSNP:750380223
1732 1732 a, t dbSNP:188749315
1744 1744 c, t dbSNP:757872424
1751 1751 a, g dbSNP:750976032
1766 1766 a, g, t dbSNP:112893627
1779 1779 a, g dbSNP:767317412
1807 1807 a, g dbSNP:533295118

Target ORF information:

RefSeq Version NM_001204285
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204285

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTG TGAGTATGAC CAGCAGCGTA
CTCTCCAGCC ACAGCCCCGG TTCAGGCTCC TCCACCACTC AGGGACAGGA TGTCACTCTG
GCCCCGGCCA CGGAACCAGC TTCAGGTTCA GCTGCCACCT GGGGACAGGA TGTCACCTCG
GTCCCAGTCA CCAGGCCAGC CCTGGGCTCC ACCACCCCGC CAGCCCACGA TGTCACCTCA
GCCCCGGACA ACAAGCCAGC CCCGGGCTCC ACCGCCCCCC CAGCCCACGG TGTCACCTCG
GCCCCGGACA CCAGGCCGGC CCCGGGCTCC ACCGCCCCCC CAGCCCATGG TGTCACCTCG
GCCCCGGACA ACAGGCCCGC CTTGGGCTCC ACCGCCCCTC CAGTCCACAA TGTCACCTCG
GCCTCAGGCT CTGCATCAGG CTCAGCTTCT ACTCTGGTGC ACAACGGCAC CTCTGCCAGG
GCTACCACAA CCCCAGCCAG CAAGAGCACT CCATTCTCAA TTCCCAGCCA CCACTCTGAT
ACTCCTACCA CCCTTGCCAG CCATAGCACC AAGACTGATG CCAGTAGCAC TCACCATAGC
ACGGTACCTC CTCTCACCTC CTCCAATCAC AGCACTTCTC CCCAGTTGTC TACTGGGGTC
TCTTTCTTTT TCCTGTCTTT TCACATTTCA AACCTCCAGT TTAATTCCTC TCTGGAAGAT
CCCAGCACCG ACTACTACCA AGAGCTGCAG AGAGACATTT CTGAAATGTT TTTGCAGATT
TATAAACAAG GGGGTTTTCT GGGCCTCTCC AATATTAAGT TCAGGCCAGG ATCTGTGGTG
GTACAATTGA CTCTGGCCTT CCGAGAAGGT ACCATCAATG TCCACGACGT GGAGACACAG
TTCAATCAGT ATAAAACGGA AGCAGCCTCT CGATATAACC TGACGATCTC AGACGTCAGC
GTGAGTGATG TGCCATTTCC TTTCTCTGCC CAGTCTGGGG CTGGGGTGCC AGGCTGGGGC
ATCGCGCTGC TGGTGCTGGT CTGTGTTCTG GTTGCGCTGG CCATTGTCTA TCTCATTGCC
TTGGCTGTCT GTCAGTGCCG CCGAAAGAAC TACGGGCAGC TGGACATCTT TCCAGCCCGG
GATACCTACC ATCCTATGAG CGAGTACCCC ACCTACCACA CCCATGGGCG CTATGTGCCC
CCTAGCAGTA CCGATCGTAG CCCCTATGAG AAGGTTTCTG CAGGTAATGG TGGCAGCAGC
CTCTCTTACA CAAACCCAGC AGTGGCAGCC ACTTCTGCCA ACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20146
Accession Version NM_001018016.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 795bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, BC120975.2 and AA425062.1. On Feb 26, 2011 this sequence version replaced gi:67189006. Summary: This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]. Transcript Variant: This variant (2) uses alternate in-frame splice sites for two exons, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY327587.1, BC120975.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTTA ATTCCTCTCT GGAAGATCCC AGCACCGACT ACTACCAAGA GCTGCAGAGA
GACATTTCTG AAATGTTTTT GCAGATTTAT AAACAAGGGG GTTTTCTGGG CCTCTCCAAT
ATTAAGTTCA GGCCAGGATC TGTGGTGGTA CAATTGACTC TGGCCTTCCG AGAAGGTACC
ATCAATGTCC ACGACGTGGA GACACAGTTC AATCAGTATA AAACGGAAGC AGCCTCTCGA
TATAACCTGA CGATCTCAGA CGTCAGCGTG AGTGATGTGC CATTTCCTTT CTCTGCCCAG
TCTGGGGCTG GGGTGCCAGG CTGGGGCATC GCGCTGCTGG TGCTGGTCTG TGTTCTGGTT
GCGCTGGCCA TTGTCTATCT CATTGCCTTG GCTGTCTGTC AGTGCCGCCG AAAGAACTAC
GGGCAGCTGG ACATCTTTCC AGCCCGGGAT ACCTACCATC CTATGAGCGA GTACCCCACC
TACCACACCC ATGGGCGCTA TGTGCCCCCT AGCAGTACCG ATCGTAGCCC CTATGAGAAG
GTTTCTGCAG GTAATGGTGG CAGCAGCCTC TCTTACACAA ACCCAGCAGT GGCAGCCACT
TCTGCCAACT TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001018016.1
CDS73..867
Misc Feature(1)178..180(+)
Misc Feature(2)205..555(+)
Misc Feature(3)370..372(+)
Misc Feature(4)388..390(+)
Misc Feature(5)424..426(+)
Misc Feature(6)496..498(+)
Misc Feature(7)670..672(+)
Misc Feature(8)706..708(+)
Misc Feature(9)724..726(+)
Misc Feature(10)733..735(+)
Misc Feature(11)769..771(+)
Misc Feature(12)778..780(+)
Misc Feature(13)784..786(+)
Misc Feature(14)784..786(+)
Misc Feature(15)826..828(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..258
Gene:MUC1
Gene Synonym:
Exon (3)259..327
Gene:MUC1
Gene Synonym:
Exon (4)328..383
Gene:MUC1
Gene Synonym:
Exon (5)384..520
Gene:MUC1
Gene Synonym:
Exon (6)521..642
Gene:MUC1
Gene Synonym:
Exon (7)643..792
Gene:MUC1
Gene Synonym:
Exon (8)793..1178
Gene:MUC1
Gene Synonym:
Translation
Position Chain Variation Link
1 1 c, t dbSNP:562400415
2 2 a, g dbSNP:11465204
20 20 c, t dbSNP:372118515
24 24 c, t dbSNP:368256181
25 25 a, g, t dbSNP:764293615
26 26 c, t dbSNP:760667690
32 32 g, t dbSNP:774386878
33 33 c, g dbSNP:770752618
35 35 a, c dbSNP:749183212
39 39 a, g dbSNP:772996200
41 41 -, t dbSNP:753898556
46 46 c, t dbSNP:769518985
48 48 c, t dbSNP:564315578
65 65 c, t dbSNP:747855021
77 77 c, t dbSNP:780700800
80 80 c, t dbSNP:376608767
81 81 a, g dbSNP:373505102
87 87 c, t dbSNP:779571432
92 92 c, g dbSNP:368800613
100 100 c, t dbSNP:759009730
103 103 c, t dbSNP:377356836
131 131 c, t dbSNP:200001039
136 136 a, c dbSNP:758495827
144 144 a, t dbSNP:753789297
146 146 a, t dbSNP:764061775
148 148 c, g dbSNP:143297835
149 149 c, t dbSNP:752564558
151 151 a, g dbSNP:767382557
152 152 c, g dbSNP:147388624
153 153 a, g dbSNP:774131502
164 164 c, t dbSNP:139437006
165 165 a, g dbSNP:4072037
167 167 a, g dbSNP:766333007
172 172 a, g dbSNP:773637715
182 182 g, t dbSNP:145108039
185 185 c, t dbSNP:145667707
187 187 a, t dbSNP:776982023
190 190 a, c dbSNP:575065522
196 196 a, g dbSNP:771925604
197 197 a, g dbSNP:201761107
200 200 a, g dbSNP:758452127
203 203 a, g dbSNP:745939858
209 209 c, t dbSNP:201186912
210 210 g, t dbSNP:544640619
212 212 c, g, t dbSNP:577380147
213 213 a, c, g dbSNP:751297222
218 218 a, c dbSNP:766031544
219 219 c, g dbSNP:199568358
222 222 a, g dbSNP:749976676
232 232 g, t dbSNP:764582269
237 237 c, t dbSNP:762318074
240 240 a, c, t dbSNP:145691584
242 242 a, c dbSNP:761000946
243 243 c, t dbSNP:200478078
258 258 c, t dbSNP:772361769
268 268 a, t dbSNP:35819649
270 270 c, t dbSNP:767259710
271 271 c, t dbSNP:759068582
273 273 c, g dbSNP:751081819
280 280 c, t dbSNP:146950322
285 285 -, c dbSNP:774368817
289 289 a, g dbSNP:765746637
293 293 a, g dbSNP:763569821
298 298 c, g dbSNP:773704188
301 301 c, g dbSNP:770183823
339 339 g, t dbSNP:745924162
351 351 a, g dbSNP:140871200
371 371 a, g dbSNP:780669552
372 372 c, t dbSNP:370819310
374 374 g, t dbSNP:781095660
384 384 a, g dbSNP:768544711
386 386 -, c dbSNP:777127574
389 389 g, t dbSNP:145224844
391 391 a, t dbSNP:373382247
405 405 a, g dbSNP:139620330
410 410 c, t dbSNP:745467701
420 420 c, t dbSNP:778454369
422 422 g, t dbSNP:756756401
426 426 a, g dbSNP:370391810
428 428 c, g dbSNP:758430992
435 435 c, t dbSNP:764490455
440 440 a, t dbSNP:532835613
445 445 a, g, t dbSNP:144273480
447 447 c, t dbSNP:759892292
448 448 a, g, t dbSNP:1611770
464 464 a, g dbSNP:200411609
465 465 a, t dbSNP:41264913
470 470 a, g dbSNP:773293207
472 472 a, g dbSNP:768505970
476 476 c, g, t dbSNP:775163504
477 477 a, g dbSNP:561379331
480 480 a, g dbSNP:745587505
485 485 a, c dbSNP:766169396
490 490 a, c dbSNP:778505736
491 491 g, t dbSNP:770482054
495 495 c, t dbSNP:748774119
503 503 c, t dbSNP:146141676
504 504 a, g dbSNP:201798179
513 513 a, c, t dbSNP:781731301
514 514 a, g dbSNP:755359583
516 516 c, t dbSNP:751875756
519 519 c, t dbSNP:766729514
520 520 a, g dbSNP:201815857
524 524 a, g dbSNP:11465207
534 534 a, t dbSNP:752680798
554 554 c, g dbSNP:199840128
557 557 a, g dbSNP:369649894
563 563 a, g dbSNP:369608212
564 564 a, g dbSNP:774139080
578 578 -, g dbSNP:761859668
580 580 a, g dbSNP:766143764
582 582 c, t dbSNP:375090196
583 583 a, g, t dbSNP:373081136
584 584 c, t dbSNP:747711729
585 585 a, g dbSNP:776240199
586 586 a, c dbSNP:769289843
592 592 a, g, t dbSNP:780622331
597 597 a, g dbSNP:758786611
598 598 c, g dbSNP:746119110
603 603 c, t dbSNP:779173931
604 604 a, g dbSNP:757373880
614 614 c, t dbSNP:200788986
615 615 a, g dbSNP:764097944
618 618 g, t dbSNP:141460657
622 622 a, g dbSNP:751488017
625 625 a, g dbSNP:766124835
629 629 a, g dbSNP:183700327
630 630 c, t dbSNP:772830298
641 641 c, t dbSNP:199768496
649 649 -, tgtc dbSNP:747704526
659 659 a, g dbSNP:764882391
661 661 c, t dbSNP:761613959
665 665 -, a dbSNP:780480243
667 667 a, g dbSNP:753535070
669 669 c, g dbSNP:763738651
672 672 c, t dbSNP:773917931
673 673 a, g dbSNP:774814129
685 685 a, g dbSNP:772667594
695 695 c, g dbSNP:557552446
697 697 c, t dbSNP:774796958
698 698 a, g dbSNP:771275191
700 700 a, g dbSNP:749581526
718 718 a, c dbSNP:777946901
720 720 c, g, t dbSNP:200639498
721 721 a, g dbSNP:768456378
725 725 a, c dbSNP:781066358
730 730 a, c dbSNP:758368648
733 733 g, t dbSNP:750312588
742 742 c, t dbSNP:778833490
743 743 a, t dbSNP:757088101
748 748 c, t dbSNP:572153326
749 749 a, g dbSNP:763861742
767 767 a, g dbSNP:760364466
771 771 a, c, t dbSNP:766884618
772 772 a, g dbSNP:760149442
775 775 c, g, t dbSNP:372682991
776 776 g, t dbSNP:191544901
785 785 a, g dbSNP:148332231
800 800 c, t dbSNP:773579272
808 808 g, t dbSNP:765571435
809 809 g, t dbSNP:762162139
821 821 c, t dbSNP:776685788
823 823 c, t dbSNP:768830740
825 825 -, tt dbSNP:779449960
828 828 c, t dbSNP:11465209
833 833 a, c dbSNP:775673556
835 835 a, c dbSNP:770998731
839 839 a, c dbSNP:749183591
842 842 c, t dbSNP:777772044
844 844 c, g dbSNP:199968990
845 845 c, g dbSNP:747879087
854 854 c, g dbSNP:780727289
861 861 c, t dbSNP:754615206
873 873 c, t dbSNP:1611772
874 874 a, g dbSNP:549073561
876 876 c, t dbSNP:369597551
877 877 a, g dbSNP:139043100
879 879 a, c dbSNP:765630606
880 880 c, t dbSNP:762060905
889 889 c, g dbSNP:777006162
893 893 a, g dbSNP:764254987
894 894 c, g, t dbSNP:775726708
907 907 a, g dbSNP:772268236
915 915 c, t dbSNP:752708990
917 917 a, g dbSNP:773256715
923 923 g, t dbSNP:769683074
946 946 c, t dbSNP:1611773
992 992 a, g dbSNP:779032625
1021 1021 c, t dbSNP:780969195
1022 1022 a, g dbSNP:756031439
1035 1035 c, t dbSNP:768203896
1037 1037 c, t dbSNP:746647874
1064 1064 a, g dbSNP:11465210
1084 1084 g, t dbSNP:750380223
1099 1099 a, t dbSNP:188749315
1111 1111 c, t dbSNP:757872424
1118 1118 a, g dbSNP:750976032
1133 1133 a, g, t dbSNP:112893627
1146 1146 a, g dbSNP:767317412
1174 1174 a, g dbSNP:533295118

Target ORF information:

RefSeq Version NM_001018016
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001018016

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTTA ATTCCTCTCT GGAAGATCCC AGCACCGACT ACTACCAAGA GCTGCAGAGA
GACATTTCTG AAATGTTTTT GCAGATTTAT AAACAAGGGG GTTTTCTGGG CCTCTCCAAT
ATTAAGTTCA GGCCAGGATC TGTGGTGGTA CAATTGACTC TGGCCTTCCG AGAAGGTACC
ATCAATGTCC ACGACGTGGA GACACAGTTC AATCAGTATA AAACGGAAGC AGCCTCTCGA
TATAACCTGA CGATCTCAGA CGTCAGCGTG AGTGATGTGC CATTTCCTTT CTCTGCCCAG
TCTGGGGCTG GGGTGCCAGG CTGGGGCATC GCGCTGCTGG TGCTGGTCTG TGTTCTGGTT
GCGCTGGCCA TTGTCTATCT CATTGCCTTG GCTGTCTGTC AGTGCCGCCG AAAGAACTAC
GGGCAGCTGG ACATCTTTCC AGCCCGGGAT ACCTACCATC CTATGAGCGA GTACCCCACC
TACCACACCC ATGGGCGCTA TGTGCCCCCT AGCAGTACCG ATCGTAGCCC CTATGAGAAG
GTTTCTGCAG GTAATGGTGG CAGCAGCCTC TCTTACACAA ACCCAGCAGT GGCAGCCACT
TCTGCCAACT TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20062
Accession Version NM_001204287.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 849bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 11 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, EF583647.1 and AA425062.1. Summary: This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]. Transcript Variant: This variant (11) uses an alternate splice site, compared to variant 1. The encoded isoform (11) is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF583647.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTGT CTACTGGGGT CTCTTTCTTT TTCCTGTCTT TTCACATTTC AAACCTCCAG
TTTAATTCCT CTCTGGAAGA TCCCAGCACC GACTACTACC AAGAGCTGCA GAGAGACATT
TCTGAAATGT TTTTGCAGAT TTATAAACAA GGGGGTTTTC TGGGCCTCTC CAATATTAAG
TTCAGGCCAG GATCTGTGGT GGTACAATTG ACTCTGGCCT TCCGAGAAGG TACCATCAAT
GTCCACGACG TGGAGACACA GTTCAATCAG TATAAAACGG AAGCAGCCTC TCGATATAAC
CTGACGATCT CAGACGTCAG CGTGAGTGAT GTGCCATTTC CTTTCTCTGC CCAGTCTGGG
GCTGGGGTGC CAGGCTGGGG CATCGCGCTG CTGGTGCTGG TCTGTGTTCT GGTTGCGCTG
GCCATTGTCT ATCTCATTGC CTTGGCTGTC TGTCAGTGCC GCCGAAAGAA CTACGGGCAG
CTGGACATCT TTCCAGCCCG GGATACCTAC CATCCTATGA GCGAGTACCC CACCTACCAC
ACCCATGGGC GCTATGTGCC CCCTAGCAGT ACCGATCGTA GCCCCTATGA GAAGGTTTCT
GCAGGTAATG GTGGCAGCAG CCTCTCTTAC ACAAACCCAG CAGTGGCAGC CACTTCTGCC
AACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001191216.1
CDS73..921
Misc Feature(1)262..609(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..258
Gene:MUC1
Gene Synonym:
Exon (3)259..381
Gene:MUC1
Gene Synonym:
Exon (4)382..437
Gene:MUC1
Gene Synonym:
Exon (5)438..574
Gene:MUC1
Gene Synonym:
Exon (6)575..696
Gene:MUC1
Gene Synonym:
Exon (7)697..846
Gene:MUC1
Gene Synonym:
Exon (8)847..1232
Gene:MUC1
Gene Synonym:
Translation
Position Chain Variation Link
1 1 c, t dbSNP:562400415
2 2 a, g dbSNP:11465204
20 20 c, t dbSNP:372118515
24 24 c, t dbSNP:368256181
25 25 a, g, t dbSNP:764293615
26 26 c, t dbSNP:760667690
32 32 g, t dbSNP:774386878
33 33 c, g dbSNP:770752618
35 35 a, c dbSNP:749183212
39 39 a, g dbSNP:772996200
41 41 -, t dbSNP:753898556
46 46 c, t dbSNP:769518985
48 48 c, t dbSNP:564315578
65 65 c, t dbSNP:747855021
77 77 c, t dbSNP:780700800
80 80 c, t dbSNP:376608767
81 81 a, g dbSNP:373505102
87 87 c, t dbSNP:779571432
92 92 c, g dbSNP:368800613
100 100 c, t dbSNP:759009730
103 103 c, t dbSNP:377356836
131 131 c, t dbSNP:200001039
136 136 a, c dbSNP:758495827
144 144 a, t dbSNP:753789297
146 146 a, t dbSNP:764061775
148 148 c, g dbSNP:143297835
149 149 c, t dbSNP:752564558
151 151 a, g dbSNP:767382557
152 152 c, g dbSNP:147388624
153 153 a, g dbSNP:774131502
164 164 c, t dbSNP:139437006
165 165 a, g dbSNP:4072037
167 167 a, g dbSNP:766333007
172 172 a, g dbSNP:773637715
182 182 g, t dbSNP:145108039
185 185 c, t dbSNP:145667707
187 187 a, t dbSNP:776982023
190 190 a, c dbSNP:575065522
196 196 a, g dbSNP:771925604
197 197 a, g dbSNP:201761107
200 200 a, g dbSNP:758452127
203 203 a, g dbSNP:745939858
209 209 c, t dbSNP:201186912
210 210 g, t dbSNP:544640619
212 212 c, g, t dbSNP:577380147
213 213 a, c, g dbSNP:751297222
218 218 a, c dbSNP:766031544
219 219 c, g dbSNP:199568358
222 222 a, g dbSNP:749976676
232 232 g, t dbSNP:764582269
237 237 c, t dbSNP:762318074
240 240 a, c, t dbSNP:145691584
242 242 a, c dbSNP:761000946
243 243 c, t dbSNP:200478078
258 258 c, t dbSNP:772361769
261 261 g, t dbSNP:771498973
265 265 a, c dbSNP:749690550
271 271 a, g, t dbSNP:770126594
274 274 c, t dbSNP:748436056
275 275 c, t dbSNP:781359601
284 284 g, t dbSNP:768665660
285 285 c, t dbSNP:747132214
290 290 c, t dbSNP:534337862
297 297 c, t dbSNP:371485486
306 306 c, t dbSNP:753660394
312 312 a, c, g dbSNP:755973313
322 322 a, t dbSNP:35819649
324 324 c, t dbSNP:767259710
325 325 c, t dbSNP:759068582
327 327 c, g dbSNP:751081819
334 334 c, t dbSNP:146950322
339 339 -, c dbSNP:774368817
343 343 a, g dbSNP:765746637
347 347 a, g dbSNP:763569821
352 352 c, g dbSNP:773704188
355 355 c, g dbSNP:770183823
393 393 g, t dbSNP:745924162
405 405 a, g dbSNP:140871200
425 425 a, g dbSNP:780669552
426 426 c, t dbSNP:370819310
428 428 g, t dbSNP:781095660
438 438 a, g dbSNP:768544711
440 440 -, c dbSNP:777127574
443 443 g, t dbSNP:145224844
445 445 a, t dbSNP:373382247
459 459 a, g dbSNP:139620330
464 464 c, t dbSNP:745467701
474 474 c, t dbSNP:778454369
476 476 g, t dbSNP:756756401
480 480 a, g dbSNP:370391810
482 482 c, g dbSNP:758430992
489 489 c, t dbSNP:764490455
494 494 a, t dbSNP:532835613
499 499 a, g, t dbSNP:144273480
501 501 c, t dbSNP:759892292
502 502 a, g, t dbSNP:1611770
518 518 a, g dbSNP:200411609
519 519 a, t dbSNP:41264913
524 524 a, g dbSNP:773293207
526 526 a, g dbSNP:768505970
530 530 c, g, t dbSNP:775163504
531 531 a, g dbSNP:561379331
534 534 a, g dbSNP:745587505
539 539 a, c dbSNP:766169396
544 544 a, c dbSNP:778505736
545 545 g, t dbSNP:770482054
549 549 c, t dbSNP:748774119
557 557 c, t dbSNP:146141676
558 558 a, g dbSNP:201798179
567 567 a, c, t dbSNP:781731301
568 568 a, g dbSNP:755359583
570 570 c, t dbSNP:751875756
573 573 c, t dbSNP:766729514
574 574 a, g dbSNP:201815857
578 578 a, g dbSNP:11465207
588 588 a, t dbSNP:752680798
608 608 c, g dbSNP:199840128
611 611 a, g dbSNP:369649894
617 617 a, g dbSNP:369608212
618 618 a, g dbSNP:774139080
632 632 -, g dbSNP:761859668
634 634 a, g dbSNP:766143764
636 636 c, t dbSNP:375090196
637 637 a, g, t dbSNP:373081136
638 638 c, t dbSNP:747711729
639 639 a, g dbSNP:776240199
640 640 a, c dbSNP:769289843
646 646 a, g, t dbSNP:780622331
651 651 a, g dbSNP:758786611
652 652 c, g dbSNP:746119110
657 657 c, t dbSNP:779173931
658 658 a, g dbSNP:757373880
668 668 c, t dbSNP:200788986
669 669 a, g dbSNP:764097944
672 672 g, t dbSNP:141460657
676 676 a, g dbSNP:751488017
679 679 a, g dbSNP:766124835
683 683 a, g dbSNP:183700327
684 684 c, t dbSNP:772830298
695 695 c, t dbSNP:199768496
703 703 -, tgtc dbSNP:747704526
713 713 a, g dbSNP:764882391
715 715 c, t dbSNP:761613959
719 719 -, a dbSNP:780480243
721 721 a, g dbSNP:753535070
723 723 c, g dbSNP:763738651
726 726 c, t dbSNP:773917931
727 727 a, g dbSNP:774814129
739 739 a, g dbSNP:772667594
749 749 c, g dbSNP:557552446
751 751 c, t dbSNP:774796958
752 752 a, g dbSNP:771275191
754 754 a, g dbSNP:749581526
772 772 a, c dbSNP:777946901
774 774 c, g, t dbSNP:200639498
775 775 a, g dbSNP:768456378
779 779 a, c dbSNP:781066358
784 784 a, c dbSNP:758368648
787 787 g, t dbSNP:750312588
796 796 c, t dbSNP:778833490
797 797 a, t dbSNP:757088101
802 802 c, t dbSNP:572153326
803 803 a, g dbSNP:763861742
821 821 a, g dbSNP:760364466
825 825 a, c, t dbSNP:766884618
826 826 a, g dbSNP:760149442
829 829 c, g, t dbSNP:372682991
830 830 g, t dbSNP:191544901
839 839 a, g dbSNP:148332231
854 854 c, t dbSNP:773579272
862 862 g, t dbSNP:765571435
863 863 g, t dbSNP:762162139
875 875 c, t dbSNP:776685788
877 877 c, t dbSNP:768830740
879 879 -, tt dbSNP:779449960
882 882 c, t dbSNP:11465209
887 887 a, c dbSNP:775673556
889 889 a, c dbSNP:770998731
893 893 a, c dbSNP:749183591
896 896 c, t dbSNP:777772044
898 898 c, g dbSNP:199968990
899 899 c, g dbSNP:747879087
908 908 c, g dbSNP:780727289
915 915 c, t dbSNP:754615206
927 927 c, t dbSNP:1611772
928 928 a, g dbSNP:549073561
930 930 c, t dbSNP:369597551
931 931 a, g dbSNP:139043100
933 933 a, c dbSNP:765630606
934 934 c, t dbSNP:762060905
943 943 c, g dbSNP:777006162
947 947 a, g dbSNP:764254987
948 948 c, g, t dbSNP:775726708
961 961 a, g dbSNP:772268236
969 969 c, t dbSNP:752708990
971 971 a, g dbSNP:773256715
977 977 g, t dbSNP:769683074
1000 1000 c, t dbSNP:1611773
1046 1046 a, g dbSNP:779032625
1075 1075 c, t dbSNP:780969195
1076 1076 a, g dbSNP:756031439
1089 1089 c, t dbSNP:768203896
1091 1091 c, t dbSNP:746647874
1118 1118 a, g dbSNP:11465210
1138 1138 g, t dbSNP:750380223
1153 1153 a, t dbSNP:188749315
1165 1165 c, t dbSNP:757872424
1172 1172 a, g dbSNP:750976032
1187 1187 a, g, t dbSNP:112893627
1200 1200 a, g dbSNP:767317412
1228 1228 a, g dbSNP:533295118

Target ORF information:

RefSeq Version NM_001204287
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204287

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTGT CTACTGGGGT CTCTTTCTTT TTCCTGTCTT TTCACATTTC AAACCTCCAG
TTTAATTCCT CTCTGGAAGA TCCCAGCACC GACTACTACC AAGAGCTGCA GAGAGACATT
TCTGAAATGT TTTTGCAGAT TTATAAACAA GGGGGTTTTC TGGGCCTCTC CAATATTAAG
TTCAGGCCAG GATCTGTGGT GGTACAATTG ACTCTGGCCT TCCGAGAAGG TACCATCAAT
GTCCACGACG TGGAGACACA GTTCAATCAG TATAAAACGG AAGCAGCCTC TCGATATAAC
CTGACGATCT CAGACGTCAG CGTGAGTGAT GTGCCATTTC CTTTCTCTGC CCAGTCTGGG
GCTGGGGTGC CAGGCTGGGG CATCGCGCTG CTGGTGCTGG TCTGTGTTCT GGTTGCGCTG
GCCATTGTCT ATCTCATTGC CTTGGCTGTC TGTCAGTGCC GCCGAAAGAA CTACGGGCAG
CTGGACATCT TTCCAGCCCG GGATACCTAC CATCCTATGA GCGAGTACCC CACCTACCAC
ACCCATGGGC GCTATGTGCC CCCTAGCAGT ACCGATCGTA GCCCCTATGA GAAGGTTTCT
GCAGGTAATG GTGGCAGCAG CCTCTCTTAC ACAAACCCAG CAGTGGCAGC CACTTCTGCC
AACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19896
Accession Version NM_001204288.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 660bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 12 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, EF583649.1 and AA425062.1. Summary: This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]. Transcript Variant: This variant (12) has multiple differences, one of which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (12) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF583649.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTTA ATTCCTCTCT GGAAGATCCC AGCACCGACT ACTACCAAGA GCTGCAGAGA
GACATTTCTG AAATGTTTTT GCAGATTTAT AAACAAGGGG GTTTTCTGGG CCTCTCCAAT
ATTAAGTTCA GGCCAGGATC TGTGGTGGTA CAATTGACTC TGGCCTTCCG AGAAGGTACC
ATCAATGTCC ACGACGTGGA GACACAGTTC AATCAGTATA AAACGGAAGC AGCCTCTCGA
TATAACCTGA CGATCTCAGA CGTCAGCGTC TGGGGCTGGG GTGCCAGGCT GGGGCATCGC
GCTGCTGGTG CTGGTCTGTG TTCTGGTTGC GCTGGCCATT GTCTATCTCA TTGCCTTGGC
TGTCTGTCAG TGCCGCCGAA AGAACTACGG GCAGCTGGAC ATCTTTCCAG CCCGGGATAC
CTACCATCCT ATGAGCGAGT ACCCCACCTA CCACACCCAT GGGCGCTATG TGCCCCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001191217.1
CDS73..732
Misc Feature(1)205..522(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..258
Gene:MUC1
Gene Synonym:
Exon (3)259..327
Gene:MUC1
Gene Synonym:
Exon (4)328..383
Gene:MUC1
Gene Synonym:
Exon (5)384..520
Gene:MUC1
Gene Synonym:
Exon (6)521..610
Gene:MUC1
Gene Synonym:
Exon (7)611..760
Gene:MUC1
Gene Synonym:
Exon (8)761..1146
Gene:MUC1
Gene Synonym:
Translation
Position Chain Variation Link
1 1 c, t dbSNP:562400415
2 2 a, g dbSNP:11465204
20 20 c, t dbSNP:372118515
24 24 c, t dbSNP:368256181
25 25 a, g, t dbSNP:764293615
26 26 c, t dbSNP:760667690
32 32 g, t dbSNP:774386878
33 33 c, g dbSNP:770752618
35 35 a, c dbSNP:749183212
39 39 a, g dbSNP:772996200
41 41 -, t dbSNP:753898556
46 46 c, t dbSNP:769518985
48 48 c, t dbSNP:564315578
65 65 c, t dbSNP:747855021
77 77 c, t dbSNP:780700800
80 80 c, t dbSNP:376608767
81 81 a, g dbSNP:373505102
87 87 c, t dbSNP:779571432
92 92 c, g dbSNP:368800613
100 100 c, t dbSNP:759009730
103 103 c, t dbSNP:377356836
131 131 c, t dbSNP:200001039
136 136 a, c dbSNP:758495827
144 144 a, t dbSNP:753789297
146 146 a, t dbSNP:764061775
148 148 c, g dbSNP:143297835
149 149 c, t dbSNP:752564558
151 151 a, g dbSNP:767382557
152 152 c, g dbSNP:147388624
153 153 a, g dbSNP:774131502
164 164 c, t dbSNP:139437006
165 165 a, g dbSNP:4072037
167 167 a, g dbSNP:766333007
172 172 a, g dbSNP:773637715
182 182 g, t dbSNP:145108039
185 185 c, t dbSNP:145667707
187 187 a, t dbSNP:776982023
190 190 a, c dbSNP:575065522
196 196 a, g dbSNP:771925604
197 197 a, g dbSNP:201761107
200 200 a, g dbSNP:758452127
203 203 a, g dbSNP:745939858
209 209 c, t dbSNP:201186912
210 210 g, t dbSNP:544640619
212 212 c, g, t dbSNP:577380147
213 213 a, c, g dbSNP:751297222
218 218 a, c dbSNP:766031544
219 219 c, g dbSNP:199568358
222 222 a, g dbSNP:749976676
232 232 g, t dbSNP:764582269
237 237 c, t dbSNP:762318074
240 240 a, c, t dbSNP:145691584
242 242 a, c dbSNP:761000946
243 243 c, t dbSNP:200478078
258 258 c, t dbSNP:772361769
268 268 a, t dbSNP:35819649
270 270 c, t dbSNP:767259710
271 271 c, t dbSNP:759068582
273 273 c, g dbSNP:751081819
280 280 c, t dbSNP:146950322
285 285 -, c dbSNP:774368817
289 289 a, g dbSNP:765746637
293 293 a, g dbSNP:763569821
298 298 c, g dbSNP:773704188
301 301 c, g dbSNP:770183823
339 339 g, t dbSNP:745924162
351 351 a, g dbSNP:140871200
371 371 a, g dbSNP:780669552
372 372 c, t dbSNP:370819310
374 374 g, t dbSNP:781095660
384 384 a, g dbSNP:768544711
386 386 -, c dbSNP:777127574
389 389 g, t dbSNP:145224844
391 391 a, t dbSNP:373382247
405 405 a, g dbSNP:139620330
410 410 c, t dbSNP:745467701
420 420 c, t dbSNP:778454369
422 422 g, t dbSNP:756756401
426 426 a, g dbSNP:370391810
428 428 c, g dbSNP:758430992
435 435 c, t dbSNP:764490455
440 440 a, t dbSNP:532835613
445 445 a, g, t dbSNP:144273480
447 447 c, t dbSNP:759892292
448 448 a, g, t dbSNP:1611770
464 464 a, g dbSNP:200411609
465 465 a, t dbSNP:41264913
470 470 a, g dbSNP:773293207
472 472 a, g dbSNP:768505970
476 476 c, g, t dbSNP:775163504
477 477 a, g dbSNP:561379331
480 480 a, g dbSNP:745587505
485 485 a, c dbSNP:766169396
490 490 a, c dbSNP:778505736
491 491 g, t dbSNP:770482054
495 495 c, t dbSNP:748774119
503 503 c, t dbSNP:146141676
504 504 a, g dbSNP:201798179
513 513 a, c, t dbSNP:781731301
514 514 a, g dbSNP:755359583
516 516 c, t dbSNP:751875756
519 519 c, t dbSNP:766729514
520 520 a, g dbSNP:201815857
522 522 c, g dbSNP:199840128
525 525 a, g dbSNP:369649894
531 531 a, g dbSNP:369608212
532 532 a, g dbSNP:774139080
546 546 -, g dbSNP:761859668
548 548 a, g dbSNP:766143764
550 550 c, t dbSNP:375090196
551 551 a, g, t dbSNP:373081136
552 552 c, t dbSNP:747711729
553 553 a, g dbSNP:776240199
554 554 a, c dbSNP:769289843
560 560 a, g, t dbSNP:780622331
565 565 a, g dbSNP:758786611
566 566 c, g dbSNP:746119110
571 571 c, t dbSNP:779173931
572 572 a, g dbSNP:757373880
582 582 c, t dbSNP:200788986
583 583 a, g dbSNP:764097944
586 586 g, t dbSNP:141460657
590 590 a, g dbSNP:751488017
593 593 a, g dbSNP:766124835
597 597 a, g dbSNP:183700327
598 598 c, t dbSNP:772830298
609 609 c, t dbSNP:199768496
617 617 -, tgtc dbSNP:747704526
627 627 a, g dbSNP:764882391
629 629 c, t dbSNP:761613959
633 633 -, a dbSNP:780480243
635 635 a, g dbSNP:753535070
637 637 c, g dbSNP:763738651
640 640 c, t dbSNP:773917931
641 641 a, g dbSNP:774814129
653 653 a, g dbSNP:772667594
663 663 c, g dbSNP:557552446
665 665 c, t dbSNP:774796958
666 666 a, g dbSNP:771275191
668 668 a, g dbSNP:749581526
686 686 a, c dbSNP:777946901
688 688 c, g, t dbSNP:200639498
689 689 a, g dbSNP:768456378
693 693 a, c dbSNP:781066358
698 698 a, c dbSNP:758368648
701 701 g, t dbSNP:750312588
710 710 c, t dbSNP:778833490
711 711 a, t dbSNP:757088101
716 716 c, t dbSNP:572153326
717 717 a, g dbSNP:763861742
735 735 a, g dbSNP:760364466
739 739 a, c, t dbSNP:766884618
740 740 a, g dbSNP:760149442
743 743 c, g, t dbSNP:372682991
744 744 g, t dbSNP:191544901
753 753 a, g dbSNP:148332231
768 768 c, t dbSNP:773579272
776 776 g, t dbSNP:765571435
777 777 g, t dbSNP:762162139
789 789 c, t dbSNP:776685788
791 791 c, t dbSNP:768830740
793 793 -, tt dbSNP:779449960
796 796 c, t dbSNP:11465209
801 801 a, c dbSNP:775673556
803 803 a, c dbSNP:770998731
807 807 a, c dbSNP:749183591
810 810 c, t dbSNP:777772044
812 812 c, g dbSNP:199968990
813 813 c, g dbSNP:747879087
822 822 c, g dbSNP:780727289
829 829 c, t dbSNP:754615206
841 841 c, t dbSNP:1611772
842 842 a, g dbSNP:549073561
844 844 c, t dbSNP:369597551
845 845 a, g dbSNP:139043100
847 847 a, c dbSNP:765630606
848 848 c, t dbSNP:762060905
857 857 c, g dbSNP:777006162
861 861 a, g dbSNP:764254987
862 862 c, g, t dbSNP:775726708
875 875 a, g dbSNP:772268236
883 883 c, t dbSNP:752708990
885 885 a, g dbSNP:773256715
891 891 g, t dbSNP:769683074
914 914 c, t dbSNP:1611773
960 960 a, g dbSNP:779032625
989 989 c, t dbSNP:780969195
990 990 a, g dbSNP:756031439
1003 1003 c, t dbSNP:768203896
1005 1005 c, t dbSNP:746647874
1032 1032 a, g dbSNP:11465210
1052 1052 g, t dbSNP:750380223
1067 1067 a, t dbSNP:188749315
1079 1079 c, t dbSNP:757872424
1086 1086 a, g dbSNP:750976032
1101 1101 a, g, t dbSNP:112893627
1114 1114 a, g dbSNP:767317412
1142 1142 a, g dbSNP:533295118

Target ORF information:

RefSeq Version NM_001204288
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204288

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTTA ATTCCTCTCT GGAAGATCCC AGCACCGACT ACTACCAAGA GCTGCAGAGA
GACATTTCTG AAATGTTTTT GCAGATTTAT AAACAAGGGG GTTTTCTGGG CCTCTCCAAT
ATTAAGTTCA GGCCAGGATC TGTGGTGGTA CAATTGACTC TGGCCTTCCG AGAAGGTACC
ATCAATGTCC ACGACGTGGA GACACAGTTC AATCAGTATA AAACGGAAGC AGCCTCTCGA
TATAACCTGA CGATCTCAGA CGTCAGCGTC TGGGGCTGGG GTGCCAGGCT GGGGCATCGC
GCTGCTGGTG CTGGTCTGTG TTCTGGTTGC GCTGGCCATT GTCTATCTCA TTGCCTTGGC
TGTCTGTCAG TGCCGCCGAA AGAACTACGG GCAGCTGGAC ATCTTTCCAG CCCGGGATAC
CTACCATCCT ATGAGCGAGT ACCCCACCTA CCACACCCAT GGGCGCTATG TGCCCCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20181
Accession Version NM_001204290.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 654bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 14 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, EF583654.1 and AA425062.1. Summary: This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]. Transcript Variant: This variant (14) has multiple differences but maintains the reading frame, compared to variant 1. The encoded isoform (14) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF583654.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGGT 
GGAGAAAAGG AGACTTCGGC TACCCAGAGA AGTTCAGTGC CCAGCTCTAC TGAGAAGAAT
GCTATTTATA AACAAGGGGG TTTTCTGGGC CTCTCCAATA TTAAGTTCAG GCCAGGATCT
GTGGTGGTAC AATTGACTCT GGCCTTCCGA GAAGGTACCA TCAATGTCCA CGACGTGGAG
ACACAGTTCA ATCAGTATAA AACGGAAGCA GCCTCTCGAT ATAACCTGAC GATCTCAGAC
GTCAGCGTGA GTGATGTGCC ATTTCCTTTC TCTGCCCAGT CTGGGGCTGG GGTGCCAGGC
TGGGGCATCG CGCTGCTGGT GCTGGTCTGT GTTCTGGTTG CGCTGGCCAT TGTCTATCTC
ATTGCCTTGG CTGTCTGTCA GTGCCGCCGA AAGAACTACG GGCAGCTGGA CATCTTTCCA
GCCCGGGATA CCTACCATCC TATGAGCGAG TACCCCACCT ACCACACCCA TGGGCGCTAT
GTGCCCCCTA GCAGTACCGA TCGTAGCCCC TATGAGAAGG TTTCTGCAGG TAATGGTGGC
AGCAGCCTCT CTTACACAAA CCCAGCAGTG GCAGCCACTT CTGCCAACTT GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001191219.1
CDS73..726
Misc Feature(1)196..414(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..195
Gene:MUC1
Gene Synonym:
Exon (3)196..242
Gene:MUC1
Gene Synonym:
Exon (4)243..379
Gene:MUC1
Gene Synonym:
Exon (5)380..501
Gene:MUC1
Gene Synonym:
Exon (6)502..651
Gene:MUC1
Gene Synonym:
Exon (7)652..1037
Gene:MUC1
Gene Synonym:
Translation
Position Chain Variation Link
1 1 c, t dbSNP:562400415
2 2 a, g dbSNP:11465204
20 20 c, t dbSNP:372118515
24 24 c, t dbSNP:368256181
25 25 a, g, t dbSNP:764293615
26 26 c, t dbSNP:760667690
32 32 g, t dbSNP:774386878
33 33 c, g dbSNP:770752618
35 35 a, c dbSNP:749183212
39 39 a, g dbSNP:772996200
41 41 -, t dbSNP:753898556
46 46 c, t dbSNP:769518985
48 48 c, t dbSNP:564315578
65 65 c, t dbSNP:747855021
77 77 c, t dbSNP:780700800
80 80 c, t dbSNP:376608767
81 81 a, g dbSNP:373505102
87 87 c, t dbSNP:779571432
92 92 c, g dbSNP:368800613
100 100 c, t dbSNP:759009730
103 103 c, t dbSNP:377356836
133 133 a, g dbSNP:771925604
134 134 a, g dbSNP:201761107
137 137 a, g dbSNP:758452127
140 140 a, g dbSNP:745939858
146 146 c, t dbSNP:201186912
147 147 g, t dbSNP:544640619
149 149 c, g, t dbSNP:577380147
150 150 a, c, g dbSNP:751297222
155 155 a, c dbSNP:766031544
156 156 c, g dbSNP:199568358
159 159 a, g dbSNP:749976676
169 169 g, t dbSNP:764582269
174 174 c, t dbSNP:762318074
177 177 a, c, t dbSNP:145691584
179 179 a, c dbSNP:761000946
180 180 c, t dbSNP:200478078
195 195 c, t dbSNP:772361769
198 198 g, t dbSNP:745924162
210 210 a, g dbSNP:140871200
230 230 a, g dbSNP:780669552
231 231 c, t dbSNP:370819310
233 233 g, t dbSNP:781095660
243 243 a, g dbSNP:768544711
245 245 -, c dbSNP:777127574
248 248 g, t dbSNP:145224844
250 250 a, t dbSNP:373382247
264 264 a, g dbSNP:139620330
269 269 c, t dbSNP:745467701
279 279 c, t dbSNP:778454369
281 281 g, t dbSNP:756756401
285 285 a, g dbSNP:370391810
287 287 c, g dbSNP:758430992
294 294 c, t dbSNP:764490455
299 299 a, t dbSNP:532835613
304 304 a, g, t dbSNP:144273480
306 306 c, t dbSNP:759892292
307 307 a, g, t dbSNP:1611770
323 323 a, g dbSNP:200411609
324 324 a, t dbSNP:41264913
329 329 a, g dbSNP:773293207
331 331 a, g dbSNP:768505970
335 335 c, g, t dbSNP:775163504
336 336 a, g dbSNP:561379331
339 339 a, g dbSNP:745587505
344 344 a, c dbSNP:766169396
349 349 a, c dbSNP:778505736
350 350 g, t dbSNP:770482054
354 354 c, t dbSNP:748774119
362 362 c, t dbSNP:146141676
363 363 a, g dbSNP:201798179
372 372 a, c, t dbSNP:781731301
373 373 a, g dbSNP:755359583
375 375 c, t dbSNP:751875756
378 378 c, t dbSNP:766729514
379 379 a, g dbSNP:201815857
383 383 a, g dbSNP:11465207
393 393 a, t dbSNP:752680798
413 413 c, g dbSNP:199840128
416 416 a, g dbSNP:369649894
422 422 a, g dbSNP:369608212
423 423 a, g dbSNP:774139080
437 437 -, g dbSNP:761859668
439 439 a, g dbSNP:766143764
441 441 c, t dbSNP:375090196
442 442 a, g, t dbSNP:373081136
443 443 c, t dbSNP:747711729
444 444 a, g dbSNP:776240199
445 445 a, c dbSNP:769289843
451 451 a, g, t dbSNP:780622331
456 456 a, g dbSNP:758786611
457 457 c, g dbSNP:746119110
462 462 c, t dbSNP:779173931
463 463 a, g dbSNP:757373880
473 473 c, t dbSNP:200788986
474 474 a, g dbSNP:764097944
477 477 g, t dbSNP:141460657
481 481 a, g dbSNP:751488017
484 484 a, g dbSNP:766124835
488 488 a, g dbSNP:183700327
489 489 c, t dbSNP:772830298
500 500 c, t dbSNP:199768496
508 508 -, tgtc dbSNP:747704526
518 518 a, g dbSNP:764882391
520 520 c, t dbSNP:761613959
524 524 -, a dbSNP:780480243
526 526 a, g dbSNP:753535070
528 528 c, g dbSNP:763738651
531 531 c, t