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MUT methylmalonyl CoA mutase [Homo sapiens (human)]

Gene Symbol MUT
Entrez Gene ID 4594
Full Name methylmalonyl CoA mutase
Synonyms MCM
General protein information
Preferred Names
methylmalonyl-CoA mutase, mitochondrial
Names
methylmalonyl-CoA mutase, mitochondrial
methylmalonyl-CoA isomerase
methylmalonyl Coenzyme A mutase
NP_000246.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p12.3

Summary This gene encodes the mitochondrial enzyme methylmalonyl Coenzyme A mutase. In humans, the product of this gene is a vitamin B12-dependent enzyme which catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA, while in other species this enzyme may have different functions. Mutations in this gene may lead to various types of methylmalonic aciduria. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

609058

Disorder Html: Methylmalonic aciduria, mut(0) type, 251000 (3)





The following MUT gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MUT gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu25184 NM_000255 Homo sapiens methylmalonyl CoA mutase (MUT), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $379
OHu25184 XM_005249143 PREDICTED: Homo sapiens methylmalonyl CoA mutase (MUT), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 Quote Price

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu25184
Accession Version NM_000255.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 04-AUG-2015
Organism Homo sapiens (human)
Product methylmalonyl-CoA mutase, mitochondrial precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA777441.1, AK292568.1, AK312611.1 and AL590668.5. This sequence is a reference standard in the RefSeqGene project. On May 13, 2010 this sequence version replaced gi:156105688. Summary: This gene encodes the mitochondrial enzyme methylmalonyl Coenzyme A mutase. In humans, the product of this gene is a vitamin B12-dependent enzyme which catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA, while in other species this enzyme may have different functions. Mutations in this gene may lead to various types of methylmalonic aciduria. [provided by RefSeq, Jul 2008]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292568.1, M65131.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTTAAGAG CTAAGAATCA GCTTTTTTTA CTTTCACCTC ATTACCTGAG GCAGGTAAAA 
GAATCATCAG GCTCCAGGCT CATACAGCAA CGACTTCTAC ACCAGCAACA GCCCCTTCAC
CCAGAATGGG CTGCCCTGGC TAAAAAGCAG CTGAAAGGCA AAAACCCAGA AGACCTAATA
TGGCACACCC CGGAAGGGAT CTCTATAAAA CCCTTGTATT CCAAGAGAGA TACTATGGAC
TTACCTGAAG AACTTCCAGG AGTGAAGCCA TTCACACGTG GACCATATCC TACCATGTAT
ACCTTTAGGC CCTGGACCAT CCGCCAGTAT GCTGGTTTTA GTACTGTGGA AGAAAGCAAT
AAGTTCTATA AGGACAACAT TAAGGCTGGT CAGCAGGGAT TATCAGTTGC CTTTGATCTG
GCGACACATC GTGGCTATGA TTCAGACAAC CCTCGAGTTC GTGGTGATGT TGGAATGGCT
GGAGTTGCTA TTGACACTGT GGAAGATACC AAAATTCTTT TTGATGGAAT TCCTTTAGAA
AAAATGTCAG TTTCCATGAC TATGAATGGA GCAGTTATTC CAGTTCTTGC AAATTTTATA
GTAACTGGAG AAGAACAAGG TGTACCTAAA GAGAAGCTTA CTGGTACCAT CCAAAATGAT
ATACTAAAGG AATTTATGGT TCGAAATACA TACATTTTTC CTCCAGAACC ATCCATGAAA
ATTATTGCTG ACATATTTGA ATATACAGCA AAGCACATGC CAAAATTTAA TTCAATTTCA
ATTAGTGGAT ACCATATGCA GGAAGCAGGG GCTGATGCCA TTCTGGAGCT GGCCTATACT
TTAGCAGATG GATTGGAGTA CTCTAGAACT GGACTCCAGG CTGGCCTGAC AATTGATGAA
TTTGCACCAA GGTTGTCTTT CTTCTGGGGA ATTGGAATGA ATTTCTATAT GGAAATAGCA
AAGATGAGAG CTGGTAGAAG ACTCTGGGCT CACTTAATAG AGAAAATGTT TCAGCCTAAA
AACTCAAAAT CTCTTCTTCT AAGAGCACAC TGTCAGACAT CTGGATGGTC ACTTACTGAG
CAGGATCCCT ACAATAATAT TGTCCGTACT GCAATAGAAG CAATGGCAGC AGTATTTGGA
GGGACTCAGT CTTTGCACAC AAATTCTTTT GATGAAGCTT TGGGTTTGCC AACTGTGAAA
AGTGCTCGAA TTGCCAGGAA CACACAAATC ATCATTCAAG AAGAATCTGG GATTCCCAAA
GTGGCTGATC CTTGGGGAGG TTCTTACATG ATGGAATGTC TCACAAATGA TGTTTATGAT
GCTGCTTTAA AGCTCATTAA TGAAATTGAA GAAATGGGTG GAATGGCCAA AGCTGTAGCT
GAGGGAATAC CTAAACTTCG AATTGAAGAA TGTGCTGCCC GAAGACAAGC TAGAATAGAT
TCTGGTTCTG AAGTAATTGT TGGAGTAAAT AAGTACCAGT TGGAAAAAGA AGACGCTGTA
GAAGTTCTGG CAATTGATAA TACTTCAGTG CGAAACAGGC AGATTGAAAA ACTTAAGAAG
ATCAAATCCA GCAGGGATCA AGCTTTGGCT GAACGTTGTC TTGCTGCACT AACCGAATGT
GCTGCTAGCG GAGATGGAAA TATCCTGGCT CTTGCAGTGG ATGCATCTCG GGCAAGATGT
ACAGTGGGAG AAATCACAGA TGCCCTGAAA AAGGTATTTG GTGAACATAA AGCGAATGAT
CGAATGGTGA GTGGAGCATA TCGCCAGGAA TTTGGAGAAA GTAAAGAGAT AACATCTGCT
ATCAAGAGGG TTCATAAATT CATGGAACGT GAAGGTCGCA GACCTCGTCT TCTTGTAGCA
AAAATGGGAC AAGATGGCCA TGACAGAGGA GCAAAAGTTA TTGCTACAGG ATTTGCTGAT
CTTGGTTTTG ATGTGGACAT AGGCCCTCTT TTCCAGACTC CTCGTGAAGT GGCCCAGCAG
GCTGTGGATG CGGATGTGCA TGCTGTGGGC ATAAGCACCC TCGCTGCTGG TCATAAAACC
CTAGTTCCTG AACTCATCAA AGAACTTAAC TCCCTTGGAC GGCCAGATAT TCTTGTCATG
TGTGGAGGGG TGATACCACC TCAGGATTAT GAATTTCTGT TTGAAGTTGG TGTTTCCAAT
GTATTTGGTC CTGGGACTCG AATTCCAAAG GCTGCCGTTC AGGTGCTTGA TGATATTGAG
AAGTGTTTGG AAAAGAAGCA GCAATCTGTA TAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000246.2
CDS266..2518
Misc Feature(1)89..91(+)
Misc Feature(2)383..1987(+)
Misc Feature(3)386..2497(+)
Misc Feature(4)530..532(+)
Misc Feature(5)551..1453(+)
Misc Feature(6)551..1414(+)
Misc Feature(7)593..1453(+)
Misc Feature(8)899..901(+)
Misc Feature(9)1268..1270(+)
Misc Feature(10)1292..1294(+)
Misc Feature(11)1706..1708(+)
Misc Feature(12)2048..2050(+)
Misc Feature(13)2069..2071(+)
Misc Feature(14)2111..2476(+)
Misc Feature(15)2135..2458(+)
Misc Feature(16)2144..2146(+)
Exon (1)1..226
Gene:MUT
Gene Synonym:
Exon (2)227..650
Gene:MUT
Gene Synonym:
Exon (3)651..1018
Gene:MUT
Gene Synonym:
Exon (4)1019..1176
Gene:MUT
Gene Synonym:
Exon (5)1177..1348
Gene:MUT
Gene Synonym:
Exon (6)1349..1597
Gene:MUT
Gene Synonym:
Exon (7)1598..1709
Gene:MUT
Gene Synonym:
Exon (8)1710..1825
Gene:MUT
Gene Synonym:
Exon (9)1826..1941
Gene:MUT
Gene Synonym:
Exon (10)1942..2073
Gene:MUT
Gene Synonym:
Exon (11)2074..2221
Gene:MUT
Gene Synonym:
Exon (12)2222..2389
Gene:MUT
Gene Synonym:
Exon (13)2390..3886
Gene:MUT
Gene Synonym:
Translation
Position Chain Variation Link
1 1 a, t dbSNP:572486772
40 40 g, t dbSNP:775452390
68 68 a, t dbSNP:3729619
75 75 c, t dbSNP:116391415
99 99 g, t dbSNP:144562919
106 106 a, c dbSNP:556798197
108 108 -, g dbSNP:145574629
131 131 g, t dbSNP:778890521
132 132 c, g dbSNP:776764666
139 139 c, t dbSNP:538802256
170 170 a, g dbSNP:200217597
187 187 a, g dbSNP:184509406
188 188 a, c dbSNP:553218776
222 222 c, t dbSNP:757176042
229 229 a, c dbSNP:483352794
231 231 g, t dbSNP:483352795
232 232 -, a dbSNP:483352797
232 232 a, t dbSNP:483352796
238 238 c, t dbSNP:756674850
243 243 a, g dbSNP:200150817
249 249 a, t dbSNP:483352789
250 250 -, gt dbSNP:775173996
250 250 g, t dbSNP:781555363
253 253 a, t dbSNP:483352784
255 255 a, c dbSNP:483352790
256 256 -, a dbSNP:769577004
256 256 a, g dbSNP:755314171
259 259 c, t dbSNP:751760653
260 260 a, t dbSNP:483352782
264 264 c, g dbSNP:766544510
265 265 c, t dbSNP:758525629
272 272 -, a dbSNP:483352781
272 272 a, t dbSNP:750583669
280 280 a, g dbSNP:483352779
283 283 a, t dbSNP:765259599
284 284 c, t dbSNP:761773115
285 285 a, g dbSNP:776721240
297 297 c, t dbSNP:763898170
300 300 c, g, t dbSNP:775177090
302 302 c, t dbSNP:771672322
305 305 a, c dbSNP:745369199
311 311 c, g dbSNP:773920536
317 317 c, t dbSNP:121918248
318 318 a, g dbSNP:372601759
321 321 g, t dbSNP:781576538
327 327 a, c dbSNP:577356836
329 329 c, t dbSNP:747378116
330 330 c, t dbSNP:780304897
341 341 a, c dbSNP:758757431
343 343 a, g dbSNP:750675392
344 344 c, g dbSNP:765349557
347 347 a, c dbSNP:778241680
356 356 c, t dbSNP:398123278
383 383 c, t dbSNP:774766479
388 388 a, g dbSNP:753769272
415 415 c, g dbSNP:368296765
426 426 a, g dbSNP:559152765
430 430 a, c dbSNP:483352780
438 438 a, c dbSNP:775206518
440 440 c, t dbSNP:767154952
441 441 g, t dbSNP:759122913
442 442 a, c dbSNP:774008307
443 443 a, c dbSNP:756504816
444 444 c, t dbSNP:770382069
448 448 a, g dbSNP:748832610
454 454 a, c dbSNP:777167901
456 456 c, t dbSNP:575038087
457 457 a, g dbSNP:747391696
461 461 a, g dbSNP:780214259
464 464 a, g dbSNP:540830529
465 465 g, t dbSNP:772575390
469 469 c, t dbSNP:746036716
470 470 a, g dbSNP:115923556
472 472 a, g dbSNP:757381606
475 475 -, a dbSNP:745805573
480 480 c, t dbSNP:753913987
485 485 g, t dbSNP:777753506
487 487 c, t dbSNP:756073255
491 491 a, g dbSNP:752686314
499 499 c, g, t dbSNP:759351751
500 500 a, g dbSNP:751177187
506 506 c, t dbSNP:150841850
510 510 c, g dbSNP:766062848
518 518 c, t dbSNP:762507663
521 521 c, t dbSNP:772660572
522 522 c, t dbSNP:769348060
523 523 a, g dbSNP:761026696
527 527 a, g dbSNP:776085460
529 529 a, g dbSNP:772522418
542 542 c, t dbSNP:746274670
543 543 a, g dbSNP:121918251
545 545 a, g dbSNP:727504022
546 546 g, t dbSNP:535411418
549 549 c, t dbSNP:190834116
550 550 a, g dbSNP:113008304
556 556 c, t dbSNP:752530461
557 557 a, c, g dbSNP:754740382
575 575 c, t dbSNP:537995137
578 578 c, t dbSNP:121918249
586 586 c, t dbSNP:570459486
587 587 c, t dbSNP:121918257
588 588 a, g dbSNP:483352778
592 592 a, g dbSNP:371185170
607 607 c, t dbSNP:761414753
614 614 g, t dbSNP:121918253
624 624 a, g dbSNP:776108716
628 628 a, g dbSNP:772753347
631 631 c, g dbSNP:368780480
635 635 a, g dbSNP:774922902
657 657 a, t dbSNP:760054029
658 658 a, g dbSNP:145682249
660 660 a, g dbSNP:766605891
685 685 a, g dbSNP:763408727
687 687 a, c, t dbSNP:565348836
688 688 a, g dbSNP:547142339
696 696 a, g dbSNP:776621768
702 702 a, g dbSNP:528689712
703 703 c, t dbSNP:200735240
714 714 a, g dbSNP:779749269
719 719 c, t dbSNP:780068818
720 720 a, g dbSNP:758095905
726 726 a, g dbSNP:142068898
729 729 a, g dbSNP:778416004
732 732 a, t dbSNP:757000253
748 748 a, g dbSNP:753545302
756 756 a, t dbSNP:201477610
759 759 a, g dbSNP:199668104
764 764 a, g dbSNP:755709278
775 775 c, g dbSNP:752074205
778 778 a, c dbSNP:200617069
797 797 c, t dbSNP:766951951
798 798 -, c dbSNP:770980538
809 809 -, a dbSNP:746556715
810 810 g, t dbSNP:763208217
811 811 c, g dbSNP:750860632
821 821 a, g dbSNP:148331800
826 826 g, t dbSNP:761961877
831 831 a, g, t dbSNP:200908035
837 837 a, c dbSNP:760782399
845 845 c, g dbSNP:775266242
851 851 a, c dbSNP:758402726
863 863 a, g dbSNP:772104231
870 870 c, t dbSNP:576792926
872 872 a, g dbSNP:778702777
880 880 a, g dbSNP:770863949
884 884 c, g dbSNP:748901340
885 885 g, t dbSNP:144034003
890 890 c, t dbSNP:755656294
892 892 g, t dbSNP:752307096
897 897 a, g dbSNP:558215803
898 898 c, g dbSNP:759014887
901 901 a, g dbSNP:2229384
902 902 c, g dbSNP:765584462
908 908 a, g dbSNP:121918258
909 909 a, g dbSNP:762193396
913 913 c, t dbSNP:754026450
917 917 c, t dbSNP:764347583
920 920 a, t dbSNP:121918256
921 921 -, a dbSNP:777296241
941 941 a, g dbSNP:775705185
943 943 -, aatttatg dbSNP:758008398
943 943 g, t dbSNP:772048660
947 947 c, t dbSNP:200596762
948 948 a, g, t dbSNP:770810987
952 952 c, t dbSNP:749137376
957 957 -, a dbSNP:747777227
976 976 a, g dbSNP:138085432
980 980 a, g dbSNP:761839867
986 986 a, g dbSNP:145586906
991 991 c, t dbSNP:747611024
994 994 -, tt dbSNP:780283588
995 995 c, g dbSNP:780691025
998 998 a, g dbSNP:754464973
1000 1000 -, at dbSNP:756533944
1000 1000 a, g dbSNP:750899217
1003 1003 a, t dbSNP:779433573
1008 1008 a, g dbSNP:757594175
1009 1009 c, t dbSNP:754257532
1011 1011 c, t dbSNP:764294717
1018 1018 c, g dbSNP:727504021
1020 1020 -, a dbSNP:751624492
1021 1021 c, t dbSNP:376716145
1030 1030 a, c dbSNP:750567657
1040 1040 a, g dbSNP:201459583
1046 1046 a, g dbSNP:375054307
1071 1071 c, t dbSNP:767593892
1076 1076 -, gg dbSNP:764189353
1081 1081 c, t dbSNP:755104984
1089 1089 g, t dbSNP:751533692
1091 1091 c, g dbSNP:766420051
1093 1093 a, g dbSNP:12175488
1096 1096 a, g dbSNP:773265096
1101 1101 a, g dbSNP:2228282
1115 1115 g, t dbSNP:761477436
1130 1130 a, g dbSNP:776371056
1131 1131 c, g dbSNP:768138141
1139 1139 c, t dbSNP:746534803
1143 1143 a, g dbSNP:138374956
1144 1144 g, t dbSNP:771557468
1155 1155 c, t dbSNP:547709692
1176 1176 a, g dbSNP:778221358
1177 1177 a, g dbSNP:201311681
1186 1186 c, t dbSNP:773728857
1196 1196 a, g dbSNP:770139557
1202 1202 a, t dbSNP:748548097
1212 1212 a, g dbSNP:781474200
1214 1214 a, g dbSNP:769047571
1215 1215 c, t dbSNP:184829210
1232 1232 -, agagctgg dbSNP:763101787
1232 1232 a, g dbSNP:575854955
1235 1235 g, t dbSNP:780387525
1242 1242 a, g dbSNP:758577372
1246 1246 a, t dbSNP:555947199
1252 1252 a, g dbSNP:750475071
1257 1257 a, g dbSNP:778915543
1265 1265 a, g dbSNP:757209646
1267 1267 a, g dbSNP:753801296
1270 1270 a, t dbSNP:763844056
1271 1271 a, g dbSNP:760343961
1273 1273 a, g dbSNP:150095448
1279 1279 a, g dbSNP:767106240
1287 1287 -, a dbSNP:752898811
1288 1288 c, t dbSNP:759057586
1290 1290 a, c, g dbSNP:770466993
1296 1296 c, g dbSNP:762281578
1297 1297 c, t dbSNP:777169651
1301 1301 a, c dbSNP:140775584
1303 1303 -, tct dbSNP:765373403
1320 1320 a, g dbSNP:747331643
1327 1327 c, t dbSNP:780440503
1341 1341 c, t dbSNP:772356159
1355 1355 a, t dbSNP:767983689
1356 1356 a, c, g dbSNP:563776413
1357 1357 c, t dbSNP:761129296
1358 1358 a, g dbSNP:776065979
1370 1370 c, t dbSNP:772552898
1371 1371 a, g dbSNP:564069299
1373 1373 a, c dbSNP:368790885
1379 1379 a, g dbSNP:770946713
1380 1380 c, t dbSNP:150968643
1381 1381 a, g dbSNP:777583211
1384 1384 a, g dbSNP:755977989
1390 1390 a, g dbSNP:148091558
1395 1395 a, c dbSNP:121918250
1399 1399 a, g dbSNP:781020628
1401 1401 g, t dbSNP:754742219
1404 1404 a, g dbSNP:751206990
1413 1413 -, a dbSNP:771021560
1427 1427 a, g dbSNP:766010704
1447 1447 a, g dbSNP:757863375
1449 1449 a, g dbSNP:749884774
1455 1455 c, t dbSNP:764838738
1470 1470 c, g dbSNP:761323724
1471 1471 g, t dbSNP:142539215
1472 1472 c, g, t dbSNP:727504020
1473 1473 a, g dbSNP:774457503
1480 1480 c, t dbSNP:771127051
1484 1484 a, t dbSNP:576536579
1485 1485 a, g dbSNP:773346301
1487 1487 a, t dbSNP:769848451
1505 1505 -, g dbSNP:760638460
1508 1508 g, t dbSNP:748129702
1511 1511 a, t dbSNP:781071952
1520 1520 a, c dbSNP:768467805
1526 1526 a, g dbSNP:746763824
1529 1529 a, g, t dbSNP:186505851
1532 1532 c, g dbSNP:750033239
1535 1535 c, t dbSNP:373381232
1541 1541 a, g dbSNP:769922244
1542 1542 c, g dbSNP:533755473
1543 1543 a, t dbSNP:756796362
1545 1545 a, g dbSNP:753288303
1551 1551 a, g dbSNP:201741770
1561 1561 a, c dbSNP:748225883
1562 1562 c, t dbSNP:375604969
1564 1564 g, t dbSNP:749158827
1570 1570 a, t dbSNP:372277433
1573 1573 c, t dbSNP:766646882
1574 1574 a, g dbSNP:763290980
1593 1593 -, t dbSNP:772609811
1609 1609 a, c dbSNP:776762981
1610 1610 a, g dbSNP:200726800
1616 1616 a, g dbSNP:768608311
1620 1620 a, t dbSNP:760718889
1623 1623 a, g dbSNP:775261191
1625 1625 a, g dbSNP:794727842
1633 1633 c, t dbSNP:771932597
1656 1656 c, t dbSNP:745609590
1664 1664 c, t dbSNP:774159791
1665 1665 a, g dbSNP:147737629
1667 1667 a, g dbSNP:145677661
1670 1670 a, g dbSNP:200850870
1674 1674 a, g dbSNP:777398959
1675 1675 a, g dbSNP:755553945
1680 1680 c, t dbSNP:747589392
1682 1682 g, t dbSNP:188766510
1686 1686 a, g dbSNP:368746965
1701 1701 c, t dbSNP:750723018
1712 1712 c, t dbSNP:772705749
1720 1720 a, g dbSNP:1803946
1725 1725 c, t dbSNP:769374584
1731 1731 a, t dbSNP:747558018
1734 1734 a, g dbSNP:780621348
1737 1737 a, c dbSNP:758975398
1738 1738 a, g dbSNP:746365541
1741 1741 c, t dbSNP:779301267
1745 1745 a, c, t dbSNP:754123075
1746 1746 a, t dbSNP:764173488
1758 1758 a, g dbSNP:377604600
1759 1759 c, t dbSNP:752651149
1760 1760 a, g dbSNP:2229385
1764 1764 c, t dbSNP:766908649
1766 1766 c, g dbSNP:759605451
1769 1769 a, g dbSNP:774407830
1776 1776 c, g dbSNP:375164316
1777 1777 a, g dbSNP:762792688
1780 1780 c, t dbSNP:772936850
1784 1784 a, g dbSNP:769270504
1796 1796 c, t dbSNP:761525156
1797 1797 a, g dbSNP:776065390
1801 1801 a, c dbSNP:768136689
1804 1804 a, g dbSNP:201483594
1817 1817 c, g dbSNP:779354089
1824 1824 a, c dbSNP:199655896
1829 1829 a, c dbSNP:748358807
1830 1830 a, c, g dbSNP:144665233
1833 1833 c, g, t dbSNP:570658958
1835 1835 a, g dbSNP:372988725
1848 1848 c, t dbSNP:750355021
1859 1859 c, t dbSNP:764985110
1860 1860 a, c, g dbSNP:1141321
1862 1862 c, t dbSNP:753512817
1863 1863 a, g dbSNP:139765234
1868 1868 c, g dbSNP:760183775
1872 1872 c, t dbSNP:369902876
1879 1879 c, t dbSNP:766999822
1880 1880 a, g dbSNP:763417266
1887 1887 c, t dbSNP:773738310
1888 1888 g, t dbSNP:770086093
1890 1890 c, t dbSNP:748492928
1893 1893 a, g dbSNP:777001813
1894 1894 c, t dbSNP:150642856
1895 1895 a, g dbSNP:78150750
1904 1904 a, g dbSNP:780334099
1909 1909 a, c dbSNP:758475503
1910 1910 c, g dbSNP:541859735
1912 1912 a, g dbSNP:745828854
1914 1914 c, t dbSNP:778864344
1924 1924 a, g dbSNP:764316430
1928 1928 a, g dbSNP:753564352
1930 1930 a, g dbSNP:760537903
1935 1935 a, g dbSNP:756389945
1937 1937 g, t dbSNP:755824133
1944 1944 -, g dbSNP:766219320
1946 1946 a, g dbSNP:766368086
1954 1954 a, g dbSNP:764587313
1969 1969 c, t dbSNP:761076380
1983 1983 c, t dbSNP:775593146
1985 1985 a, g dbSNP:772269889
1998 1998 c, t dbSNP:759674071
1999 1999 a, g dbSNP:139323928
2002 2002 c, t dbSNP:770895512
2007 2007 a, g dbSNP:749261153
2016 2016 c, g dbSNP:777647799
2023 2023 a, g dbSNP:201138975
2027 2027 c, t dbSNP:140727018
2028 2028 a, g, t dbSNP:141829043
2042 2042 c, g dbSNP:148285323
2047 2047 g, t dbSNP:779657788
2051 2051 a, g dbSNP:545334525
2054 2054 a, t dbSNP:754422552
2057 2057 a, c, g dbSNP:9473556
2066 2066 a, g dbSNP:201888226
2074 2074 g, t dbSNP:756743196
2075 2075 g, t dbSNP:753123265
2078 2078 c, t dbSNP:767966332
2079 2079 a, g dbSNP:755256821
2082 2082 a, g dbSNP:751828125
2093 2093 c, t dbSNP:770978452
2094 2094 a, g dbSNP:766529896
2102 2102 c, t dbSNP:573727541
2103 2103 a, g dbSNP:368690932
2107 2107 a, g dbSNP:200996892
2111 2111 c, t dbSNP:765284825
2117 2117 -, ctt dbSNP:398123277
2130 2130 c, t dbSNP:201536536
2132 2132 a, g dbSNP:121918254
2136 2136 a, g dbSNP:768521956
2145 2145 a, g dbSNP:372486357
2151 2151 a, g dbSNP:775291072
2154 2154 a, g dbSNP:143023066
2159 2159 a, t dbSNP:745503019
2163 2163 g, t dbSNP:200055428
2165 2165 a, g dbSNP:778617168
2166 2166 g, t dbSNP:756798250
2171 2171 a, g dbSNP:569669261
2174 2174 a, g dbSNP:781501004
2189 2189 c, g dbSNP:747897332
2203 2203 c, t dbSNP:755382704
2207 2207 a, g dbSNP:751804284
2208 2208 a, g dbSNP:766721811
2209 2209 a, c dbSNP:758674900
2211 2211 -, gccc dbSNP:760692554
2217 2217 c, t dbSNP:1136145
2224 2224 c, t dbSNP:149460386
2228 2228 c, t dbSNP:541001298
2229 2229 a, c, g dbSNP:567485663
2234 2234 g, t dbSNP:370718165
2239 2239 c, t dbSNP:757366369
2256 2256 c, t dbSNP:137958217
2257 2257 a, g dbSNP:144146728
2260 2260 c, t dbSNP:760579526
2261 2261 g, t dbSNP:776430285
2262 2262 c, t dbSNP:767453976
2270 2270 a, g dbSNP:752634070
2274 2274 c, g dbSNP:759354207
2276 2276 a, g dbSNP:8589
2280 2280 a, g dbSNP:770423282
2287 2287 c, g, t dbSNP:772652266
2288 2288 a, g dbSNP:367850243
2290 2290 c, t dbSNP:373708083
2291 2291 a, g dbSNP:147715336
2298 2298 a, g dbSNP:147094927
2306 2306 c, t dbSNP:772442006
2311 2311 c, t dbSNP:201456803
2317 2317 a, g dbSNP:746167463
2320 2320 -, ctc dbSNP:565376922
2327 2327 g, t dbSNP:779331475
2336 2336 g, t dbSNP:757478500
2343 2343 g, t dbSNP:754072258
2345 2345 c, t dbSNP:777758903
2346 2346 a, g dbSNP:756225782
2347 2347 a, g dbSNP:752705830
2349 2349 c, t dbSNP:561197473
2352 2352 a, c dbSNP:759407117
2358 2358 a, t dbSNP:751462903
2364 2364 a, t dbSNP:140600746
2372 2372 c, g dbSNP:121918255
2379 2379 c, t dbSNP:772888575
2388 2388 a, g dbSNP:769217429
2395 2395 c, t dbSNP:375956047
2396 2396 g, t dbSNP:776176938
2402 2402 a, c dbSNP:188796821
2411 2411 a, g dbSNP:763572961
2413 2413 -, tggt dbSNP:748892245
2414 2414 c, g dbSNP:760079537
2415 2415 g, t dbSNP:121918252
2423 2423 a, g dbSNP:771224892
2425 2425 c, t dbSNP:749610593
2426 2426 a, g, t dbSNP:770205649
2427 2427 c, t dbSNP:748363752
2428 2428 a, g dbSNP:781466735
2433 2433 a, g dbSNP:755077681
2441 2441 a, t dbSNP:746950479
2444 2444 c, t dbSNP:779990936
2456 2456 a, g dbSNP:758134896
2457 2457 -, ggc dbSNP:781553746
2458 2458 -, t, tg dbSNP:747331003
2460 2460 -, cc dbSNP:778402274
2460 2460 a, c dbSNP:750300062
2461 2461 c, t dbSNP:201963242
2462 2462 -, aa dbSNP:758811462
2470 2470 a, g dbSNP:756952588
2471 2471 c, t dbSNP:753461919
2474 2474 c, g dbSNP:763629567
2483 2483 a, g dbSNP:372245965
2507 2507 c, t dbSNP:760186185
2515 2515 a, t dbSNP:774736275
2521 2521 c, t dbSNP:766839518
2522 2522 c, t dbSNP:763361965
2529 2529 c, t dbSNP:773680972
2548 2548 a, g dbSNP:14341
2552 2552 a, g dbSNP:199933641
2567 2567 a, t dbSNP:762165830
2569 2569 c, g dbSNP:199521080
2587 2587 c, t dbSNP:560764129
2591 2591 g, t dbSNP:548852414
2608 2608 a, g dbSNP:530424880
2619 2619 a, g dbSNP:113025987
2626 2626 a, c dbSNP:1803945
2644 2644 a, g dbSNP:192605776
2659 2659 c, t dbSNP:576032707
2692 2692 -, ca dbSNP:764175806
2711 2711 a, t dbSNP:76470114
2719 2719 -, a dbSNP:10713340
2734 2734 c, g dbSNP:764166500
2775 2775 a, c dbSNP:187747098
2796 2796 a, g dbSNP:182726681
2797 2797 c, t dbSNP:192552805
2798 2798 a, g dbSNP:761107295
2799 2799 a, g dbSNP:144168425
2821 2821 c, t dbSNP:113362290
2824 2824 c, t dbSNP:541575016
2833 2833 c, g dbSNP:1141333
2878 2878 -, gact dbSNP:576059123
2892 2892 c, g dbSNP:1141334
2928 2928 a, g dbSNP:776011450
2947 2947 a, t dbSNP:1141335
2969 2969 c, g dbSNP:558085628
2984 2984 a, g dbSNP:538293537
2991 2991 a, g dbSNP:569368840
3034 3034 a, g dbSNP:535324778
3047 3047 a, g dbSNP:187987243
3063 3063 a, c dbSNP:74854132
3076 3076 a, g dbSNP:111322712
3087 3087 c, t dbSNP:759680109
3093 3093 a, c, g dbSNP:190188528
3098 3098 a, g dbSNP:11757098
3118 3118 a, g dbSNP:552751982
3145 3145 c, t dbSNP:534290000
3199 3199 c, t dbSNP:186205803
3216 3216 c, g dbSNP:548813379
3315 3315 g, t dbSNP:377179859
3327 3327 a, t dbSNP:530299655
3344 3344 a, g dbSNP:139283545
3352 3352 c, t dbSNP:771269097
3398 3398 -, a dbSNP:777905388
3413 3413 a, g dbSNP:563222609
3427 3427 c, t dbSNP:761072490
3434 3434 c, t dbSNP:773671452
3437 3437 g, t dbSNP:772592770
3462 3462 a, g dbSNP:748775102
3530 3530 -, gat dbSNP:752978672
3562 3562 a, t dbSNP:779436906
3600 3600 -, cag dbSNP:756006397
3611 3611 a, g dbSNP:146257268
3625 3625 -, a dbSNP:71777574
3658 3658 a, g dbSNP:566780363
3693 3693 a, g dbSNP:531378878
3701 3701 a, g dbSNP:563837981
3702 3702 c, t dbSNP:745494100
3727 3727 a, g dbSNP:545448500
3743 3743 a, g dbSNP:571818982
3763 3763 a, g dbSNP:559882082
3770 3770 c, t dbSNP:781039564
3837 3837 c, t dbSNP:541645779
3844 3844 a, g dbSNP:9381784
3870 3870 c, t dbSNP:769328040
3875 3875 g, t dbSNP:747868663

Target ORF information:

RefSeq Version NM_000255
Organism Homo sapiens (human)
Definition Homo sapiens methylmalonyl CoA mutase (MUT), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000255

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTTAAGAG CTAAGAATCA GCTTTTTTTA CTTTCACCTC ATTACCTGAG GCAGGTAAAA 
GAATCATCAG GCTCCAGGCT CATACAGCAA CGACTTCTAC ACCAGCAACA GCCCCTTCAC
CCAGAATGGG CTGCCCTGGC TAAAAAGCAG CTGAAAGGCA AAAACCCAGA AGACCTAATA
TGGCACACCC CGGAAGGGAT CTCTATAAAA CCCTTGTATT CCAAGAGAGA TACTATGGAC
TTACCTGAAG AACTTCCAGG AGTGAAGCCA TTCACACGTG GACCATATCC TACCATGTAT
ACCTTTAGGC CCTGGACCAT CCGCCAGTAT GCTGGTTTTA GTACTGTGGA AGAAAGCAAT
AAGTTCTATA AGGACAACAT TAAGGCTGGT CAGCAGGGAT TATCAGTTGC CTTTGATCTG
GCGACACATC GTGGCTATGA TTCAGACAAC CCTCGAGTTC GTGGTGATGT TGGAATGGCT
GGAGTTGCTA TTGACACTGT GGAAGATACC AAAATTCTTT TTGATGGAAT TCCTTTAGAA
AAAATGTCAG TTTCCATGAC TATGAATGGA GCAGTTATTC CAGTTCTTGC AAATTTTATA
GTAACTGGAG AAGAACAAGG TGTACCTAAA GAGAAGCTTA CTGGTACCAT CCAAAATGAT
ATACTAAAGG AATTTATGGT TCGAAATACA TACATTTTTC CTCCAGAACC ATCCATGAAA
ATTATTGCTG ACATATTTGA ATATACAGCA AAGCACATGC CAAAATTTAA TTCAATTTCA
ATTAGTGGAT ACCATATGCA GGAAGCAGGG GCTGATGCCA TTCTGGAGCT GGCCTATACT
TTAGCAGATG GATTGGAGTA CTCTAGAACT GGACTCCAGG CTGGCCTGAC AATTGATGAA
TTTGCACCAA GGTTGTCTTT CTTCTGGGGA ATTGGAATGA ATTTCTATAT GGAAATAGCA
AAGATGAGAG CTGGTAGAAG ACTCTGGGCT CACTTAATAG AGAAAATGTT TCAGCCTAAA
AACTCAAAAT CTCTTCTTCT AAGAGCACAC TGTCAGACAT CTGGATGGTC ACTTACTGAG
CAGGATCCCT ACAATAATAT TGTCCGTACT GCAATAGAAG CAATGGCAGC AGTATTTGGA
GGGACTCAGT CTTTGCACAC AAATTCTTTT GATGAAGCTT TGGGTTTGCC AACTGTGAAA
AGTGCTCGAA TTGCCAGGAA CACACAAATC ATCATTCAAG AAGAATCTGG GATTCCCAAA
GTGGCTGATC CTTGGGGAGG TTCTTACATG ATGGAATGTC TCACAAATGA TGTTTATGAT
GCTGCTTTAA AGCTCATTAA TGAAATTGAA GAAATGGGTG GAATGGCCAA AGCTGTAGCT
GAGGGAATAC CTAAACTTCG AATTGAAGAA TGTGCTGCCC GAAGACAAGC TAGAATAGAT
TCTGGTTCTG AAGTAATTGT TGGAGTAAAT AAGTACCAGT TGGAAAAAGA AGACGCTGTA
GAAGTTCTGG CAATTGATAA TACTTCAGTG CGAAACAGGC AGATTGAAAA ACTTAAGAAG
ATCAAATCCA GCAGGGATCA AGCTTTGGCT GAACGTTGTC TTGCTGCACT AACCGAATGT
GCTGCTAGCG GAGATGGAAA TATCCTGGCT CTTGCAGTGG ATGCATCTCG GGCAAGATGT
ACAGTGGGAG AAATCACAGA TGCCCTGAAA AAGGTATTTG GTGAACATAA AGCGAATGAT
CGAATGGTGA GTGGAGCATA TCGCCAGGAA TTTGGAGAAA GTAAAGAGAT AACATCTGCT
ATCAAGAGGG TTCATAAATT CATGGAACGT GAAGGTCGCA GACCTCGTCT TCTTGTAGCA
AAAATGGGAC AAGATGGCCA TGACAGAGGA GCAAAAGTTA TTGCTACAGG ATTTGCTGAT
CTTGGTTTTG ATGTGGACAT AGGCCCTCTT TTCCAGACTC CTCGTGAAGT GGCCCAGCAG
GCTGTGGATG CGGATGTGCA TGCTGTGGGC ATAAGCACCC TCGCTGCTGG TCATAAAACC
CTAGTTCCTG AACTCATCAA AGAACTTAAC TCCCTTGGAC GGCCAGATAT TCTTGTCATG
TGTGGAGGGG TGATACCACC TCAGGATTAT GAATTTCTGT TTGAAGTTGG TGTTTCCAAT
GTATTTGGTC CTGGGACTCG AATTCCAAAG GCTGCCGTTC AGGTGCTTGA TGATATTGAG
AAGTGTTTGG AAAAGAAGCA GCAATCTGTA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25184
Accession Version XM_005249143.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product methylmalonyl-CoA mutase, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007592.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced gi:530382072. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTTAAGAG CTAAGAATCA GCTTTTTTTA CTTTCACCTC ATTACCTGAG GCAGGTAAAA 
GAATCATCAG GCTCCAGGCT CATACAGCAA CGACTTCTAC ACCAGCAACA GCCCCTTCAC
CCAGAATGGG CTGCCCTGGC TAAAAAGCAG CTGAAAGGCA AAAACCCAGA AGACCTAATA
TGGCACACCC CGGAAGGGAT CTCTATAAAA CCCTTGTATT CCAAGAGAGA TACTATGGAC
TTACCTGAAG AACTTCCAGG AGTGAAGCCA TTCACACGTG GACCATATCC TACCATGTAT
ACCTTTAGGC CCTGGACCAT CCGCCAGTAT GCTGGTTTTA GTACTGTGGA AGAAAGCAAT
AAGTTCTATA AGGACAACAT TAAGGCTGGT CAGCAGGGAT TATCAGTTGC CTTTGATCTG
GCGACACATC GTGGCTATGA TTCAGACAAC CCTCGAGTTC GTGGTGATGT TGGAATGGCT
GGAGTTGCTA TTGACACTGT GGAAGATACC AAAATTCTTT TTGATGGAAT TCCTTTAGAA
AAAATGTCAG TTTCCATGAC TATGAATGGA GCAGTTATTC CAGTTCTTGC AAATTTTATA
GTAACTGGAG AAGAACAAGG TGTACCTAAA GAGAAGCTTA CTGGTACCAT CCAAAATGAT
ATACTAAAGG AATTTATGGT TCGAAATACA TACATTTTTC CTCCAGAACC ATCCATGAAA
ATTATTGCTG ACATATTTGA ATATACAGCA AAGCACATGC CAAAATTTAA TTCAATTTCA
ATTAGTGGAT ACCATATGCA GGAAGCAGGG GCTGATGCCA TTCTGGAGCT GGCCTATACT
TTAGCAGATG GATTGGAGTA CTCTAGAACT GGACTCCAGG CTGGCCTGAC AATTGATGAA
TTTGCACCAA GGTTGTCTTT CTTCTGGGGA ATTGGAATGA ATTTCTATAT GGAAATAGCA
AAGATGAGAG CTGGTAGAAG ACTCTGGGCT CACTTAATAG AGAAAATGTT TCAGCCTAAA
AACTCAAAAT CTCTTCTTCT AAGAGCACAC TGTCAGACAT CTGGATGGTC ACTTACTGAG
CAGGATCCCT ACAATAATAT TGTCCGTACT GCAATAGAAG CAATGGCAGC AGTATTTGGA
GGGACTCAGT CTTTGCACAC AAATTCTTTT GATGAAGCTT TGGGTTTGCC AACTGTGAAA
AGTGCTCGAA TTGCCAGGAA CACACAAATC ATCATTCAAG AAGAATCTGG GATTCCCAAA
GTGGCTGATC CTTGGGGAGG TTCTTACATG ATGGAATGTC TCACAAATGA TGTTTATGAT
GCTGCTTTAA AGCTCATTAA TGAAATTGAA GAAATGGGTG GAATGGCCAA AGCTGTAGCT
GAGGGAATAC CTAAACTTCG AATTGAAGAA TGTGCTGCCC GAAGACAAGC TAGAATAGAT
TCTGGTTCTG AAGTAATTGT TGGAGTAAAT AAGTACCAGT TGGAAAAAGA AGACGCTGTA
GAAGTTCTGG CAATTGATAA TACTTCAGTG CGAAACAGGC AGATTGAAAA ACTTAAGAAG
ATCAAATCCA GCAGGGATCA AGCTTTGGCT GAACGTTGTC TTGCTGCACT AACCGAATGT
GCTGCTAGCG GAGATGGAAA TATCCTGGCT CTTGCAGTGG ATGCATCTCG GGCAAGATGT
ACAGTGGGAG AAATCACAGA TGCCCTGAAA AAGGTATTTG GTGAACATAA AGCGAATGAT
CGAATGGTGA GTGGAGCATA TCGCCAGGAA TTTGGAGAAA GTAAAGAGAT AACATCTGCT
ATCAAGAGGG TTCATAAATT CATGGAACGT GAAGGTCGCA GACCTCGTCT TCTTGTAGCA
AAAATGGGAC AAGATGGCCA TGACAGAGGA GCAAAAGTTA TTGCTACAGG ATTTGCTGAT
CTTGGTTTTG ATGTGGACAT AGGCCCTCTT TTCCAGACTC CTCGTGAAGT GGCCCAGCAG
GCTGTGGATG CGGATGTGCA TGCTGTGGGC ATAAGCACCC TCGCTGCTGG TCATAAAACC
CTAGTTCCTG AACTCATCAA AGAACTTAAC TCCCTTGGAC GGCCAGATAT TCTTGTCATG
TGTGGAGGGG TGATACCACC TCAGGATTAT GAATTTCTGT TTGAAGTTGG TGTTTCCAAT
GTATTTGGTC CTGGGACTCG AATTCCAAAG GCTGCCGTTC AGGTGCTTGA TGATATTGAG
AAGTGTTTGG AAAAGAAGCA GCAATCTGTA TAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005249200.1
CDS171..2423
Misc Feature(1)288..1892(+)
Misc Feature(2)291..2402(+)
Misc Feature(3)456..1358(+)
Misc Feature(4)456..1319(+)
Misc Feature(5)498..1358(+)
Misc Feature(6)2016..2381(+)
Misc Feature(7)2040..2363(+)
Misc Feature(8)2049..2051(+)
Translation
Position Chain Variation Link
10 10 g, t dbSNP:775452390
38 38 a, t dbSNP:3729619
45 45 c, t dbSNP:116391415
69 69 g, t dbSNP:144562919
76 76 a, c dbSNP:556798197
78 78 -, g dbSNP:145574629
101 101 g, t dbSNP:778890521
102 102 c, g dbSNP:776764666
109 109 c, t dbSNP:538802256
134 134 a, c dbSNP:483352794
136 136 g, t dbSNP:483352795
137 137 -, a dbSNP:483352797
137 137 a, t dbSNP:483352796
143 143 c, t dbSNP:756674850
148 148 a, g dbSNP:200150817
154 154 a, t dbSNP:483352789
155 155 -, gt dbSNP:775173996
155 155 g, t dbSNP:781555363
158 158 a, t dbSNP:483352784
160 160 a, c dbSNP:483352790
161 161 -, a dbSNP:769577004
161 161 a, g dbSNP:755314171
164 164 c, t dbSNP:751760653
165 165 a, t dbSNP:483352782
169 169 c, g dbSNP:766544510
170 170 c, t dbSNP:758525629
177 177 -, a dbSNP:483352781
177 177 a, t dbSNP:750583669
185 185 a, g dbSNP:483352779
188 188 a, t dbSNP:765259599
189 189 c, t dbSNP:761773115
190 190 a, g dbSNP:776721240
202 202 c, t dbSNP:763898170
205 205 c, g, t dbSNP:775177090
207 207 c, t dbSNP:771672322
210 210 a, c dbSNP:745369199
216 216 c, g dbSNP:773920536
222 222 c, t dbSNP:121918248
223 223 a, g dbSNP:372601759
226 226 g, t dbSNP:781576538
232 232 a, c dbSNP:577356836
234 234 c, t dbSNP:747378116
235 235 c, t dbSNP:780304897
246 246 a, c dbSNP:758757431
248 248 a, g dbSNP:750675392
249 249 c, g dbSNP:765349557
252 252 a, c dbSNP:778241680
261 261 c, t dbSNP:398123278
288 288 c, t dbSNP:774766479
293 293 a, g dbSNP:753769272
320 320 c, g dbSNP:368296765
331 331 a, g dbSNP:559152765
335 335 a, c dbSNP:483352780
343 343 a, c dbSNP:775206518
345 345 c, t dbSNP:767154952
346 346 g, t dbSNP:759122913
347 347 a, c dbSNP:774008307
348 348 a, c dbSNP:756504816
349 349 c, t dbSNP:770382069
353 353 a, g dbSNP:748832610
359 359 a, c dbSNP:777167901
361 361 c, t dbSNP:575038087
362 362 a, g dbSNP:747391696
366 366 a, g dbSNP:780214259
369 369 a, g dbSNP:540830529
370 370 g, t dbSNP:772575390
374 374 c, t dbSNP:746036716
375 375 a, g dbSNP:115923556
377 377 a, g dbSNP:757381606
380 380 -, a dbSNP:745805573
385 385 c, t dbSNP:753913987
390 390 g, t dbSNP:777753506
392 392 c, t dbSNP:756073255
396 396 a, g dbSNP:752686314
404 404 c, g, t dbSNP:759351751
405 405 a, g dbSNP:751177187
411 411 c, t dbSNP:150841850
415 415 c, g dbSNP:766062848
423 423 c, t dbSNP:762507663
426 426 c, t dbSNP:772660572
427 427 c, t dbSNP:769348060
428 428 a, g dbSNP:761026696
432 432 a, g dbSNP:776085460
434 434 a, g dbSNP:772522418
447 447 c, t dbSNP:746274670
448 448 a, g dbSNP:121918251
450 450 a, g dbSNP:727504022
451 451 g, t dbSNP:535411418
454 454 c, t dbSNP:190834116
455 455 a, g dbSNP:113008304
461 461 c, t dbSNP:752530461
462 462 a, c, g dbSNP:754740382
480 480 c, t dbSNP:537995137
483 483 c, t dbSNP:121918249
491 491 c, t dbSNP:570459486
492 492 c, t dbSNP:121918257
493 493 a, g dbSNP:483352778
497 497 a, g dbSNP:371185170
512 512 c, t dbSNP:761414753
519 519 g, t dbSNP:121918253
529 529 a, g dbSNP:776108716
533 533 a, g dbSNP:772753347
536 536 c, g dbSNP:368780480
540 540 a, g dbSNP:774922902
562 562 a, t dbSNP:760054029
563 563 a, g dbSNP:145682249
565 565 a, g dbSNP:766605891
590 590 a, g dbSNP:763408727
592 592 a, c, t dbSNP:565348836
593 593 a, g dbSNP:547142339
601 601 a, g dbSNP:776621768
607 607 a, g dbSNP:528689712
608 608 c, t dbSNP:200735240
619 619 a, g dbSNP:779749269
624 624 c, t dbSNP:780068818
625 625 a, g dbSNP:758095905
631 631 a, g dbSNP:142068898
634 634 a, g dbSNP:778416004
637 637 a, t dbSNP:757000253
653 653 a, g dbSNP:753545302
661 661 a, t dbSNP:201477610
664 664 a, g dbSNP:199668104
669 669 a, g dbSNP:755709278
680 680 c, g dbSNP:752074205
683 683 a, c dbSNP:200617069
702 702 c, t dbSNP:766951951
703 703 -, c dbSNP:770980538
714 714 -, a dbSNP:746556715
715 715 g, t dbSNP:763208217
716 716 c, g dbSNP:750860632
726 726 a, g dbSNP:148331800
731 731 g, t dbSNP:761961877
736 736 a, g, t dbSNP:200908035
742 742 a, c dbSNP:760782399
750 750 c, g dbSNP:775266242
756 756 a, c dbSNP:758402726
768 768 a, g dbSNP:772104231
775 775 c, t dbSNP:576792926
777 777 a, g dbSNP:778702777
785 785 a, g dbSNP:770863949
789 789 c, g dbSNP:748901340
790 790 g, t dbSNP:144034003
795 795 c, t dbSNP:755656294
797 797 g, t dbSNP:752307096
802 802 a, g dbSNP:558215803
803 803 c, g dbSNP:759014887
806 806 a, g dbSNP:2229384
807 807 c, g dbSNP:765584462
813 813 a, g dbSNP:121918258
814 814 a, g dbSNP:762193396
818 818 c, t dbSNP:754026450
822 822 c, t dbSNP:764347583
825 825 a, t dbSNP:121918256
826 826 -, a dbSNP:777296241
846 846 a, g dbSNP:775705185
848 848 -, aatttatg dbSNP:758008398
848 848 g, t dbSNP:772048660
852 852 c, t dbSNP:200596762
853 853 a, g, t dbSNP:770810987
857 857 c, t dbSNP:749137376
862 862 -, a dbSNP:747777227
881 881 a, g dbSNP:138085432
885 885 a, g dbSNP:761839867
891 891 a, g dbSNP:145586906
896 896 c, t dbSNP:747611024
899 899 -, tt dbSNP:780283588
900 900 c, g dbSNP:780691025
903 903 a, g dbSNP:754464973
905 905 -, at dbSNP:756533944
905 905 a, g dbSNP:750899217
908 908 a, t dbSNP:779433573
913 913 a, g dbSNP:757594175
914 914 c, t dbSNP:754257532
916 916 c, t dbSNP:764294717
923 923 c, g dbSNP:727504021
925 925 -, a dbSNP:751624492
926 926 c, t dbSNP:376716145
935 935 a, c dbSNP:750567657
945 945 a, g dbSNP:201459583
951 951 a, g dbSNP:375054307
976 976 c, t dbSNP:767593892
981 981 -, gg dbSNP:764189353
986 986 c, t dbSNP:755104984
994 994 g, t dbSNP:751533692
996 996 c, g dbSNP:766420051
998 998 a, g dbSNP:12175488
1001 1001 a, g dbSNP:773265096
1006 1006 a, g dbSNP:2228282
1020 1020 g, t dbSNP:761477436
1035 1035 a, g dbSNP:776371056
1036 1036 c, g dbSNP:768138141
1044 1044 c, t dbSNP:746534803
1048 1048 a, g dbSNP:138374956
1049 1049 g, t dbSNP:771557468
1060 1060 c, t dbSNP:547709692
1081 1081 a, g dbSNP:778221358
1082 1082 a, g dbSNP:201311681
1091 1091 c, t dbSNP:773728857
1101 1101 a, g dbSNP:770139557
1107 1107 a, t dbSNP:748548097
1117 1117 a, g dbSNP:781474200
1119 1119 a, g dbSNP:769047571
1120 1120 c, t dbSNP:184829210
1137 1137 -, agagctgg dbSNP:763101787
1137 1137 a, g dbSNP:575854955
1140 1140 g, t dbSNP:780387525
1147 1147 a, g dbSNP:758577372
1151 1151 a, t dbSNP:555947199
1157 1157 a, g dbSNP:750475071
1162 1162 a, g dbSNP:778915543
1170 1170 a, g dbSNP:757209646
1172 1172 a, g dbSNP:753801296
1175 1175 a, t dbSNP:763844056
1176 1176 a, g dbSNP:760343961
1178 1178 a, g dbSNP:150095448
1184 1184 a, g dbSNP:767106240
1192 1192 -, a dbSNP:752898811
1193 1193 c, t dbSNP:759057586
1195 1195 a, c, g dbSNP:770466993
1201 1201 c, g dbSNP:762281578
1202 1202 c, t dbSNP:777169651
1206 1206 a, c dbSNP:140775584
1208 1208 -, tct dbSNP:765373403
1225 1225 a, g dbSNP:747331643
1232 1232 c, t dbSNP:780440503
1246 1246 c, t dbSNP:772356159
1260 1260 a, t dbSNP:767983689
1261 1261 a, c, g dbSNP:563776413
1262 1262 c, t dbSNP:761129296
1263 1263 a, g dbSNP:776065979
1275 1275 c, t dbSNP:772552898
1276 1276 a, g dbSNP:564069299
1278 1278 a, c dbSNP:368790885
1284 1284 a, g dbSNP:770946713
1285 1285 c, t dbSNP:150968643
1286 1286 a, g dbSNP:777583211
1289 1289 a, g dbSNP:755977989
1295 1295 a, g dbSNP:148091558
1300 1300 a, c dbSNP:121918250
1304 1304 a, g dbSNP:781020628
1306 1306 g, t dbSNP:754742219
1309 1309 a, g dbSNP:751206990
1318 1318 -, a dbSNP:771021560
1332 1332 a, g dbSNP:766010704
1352 1352 a, g dbSNP:757863375
1354 1354 a, g dbSNP:749884774
1360 1360 c, t dbSNP:764838738
1375 1375 c, g dbSNP:761323724
1376 1376 g, t dbSNP:142539215
1377 1377 c, g, t dbSNP:727504020
1378 1378 a, g dbSNP:774457503
1385 1385 c, t dbSNP:771127051
1389 1389 a, t dbSNP:576536579
1390 1390 a, g dbSNP:773346301
1392 1392 a, t dbSNP:769848451
1410 1410 -, g dbSNP:760638460
1413 1413 g, t dbSNP:748129702
1416 1416 a, t dbSNP:781071952
1425 1425 a, c dbSNP:768467805
1431 1431 a, g dbSNP:746763824
1434 1434 a, g, t dbSNP:186505851
1437 1437 c, g dbSNP:750033239
1440 1440 c, t dbSNP:373381232
1446 1446 a, g dbSNP:769922244
1447 1447 c, g dbSNP:533755473
1448 1448 a, t dbSNP:756796362
1450 1450 a, g dbSNP:753288303
1456 1456 a, g dbSNP:201741770
1466 1466 a, c dbSNP:748225883
1467 1467 c, t dbSNP:375604969
1469 1469 g, t dbSNP:749158827
1475 1475 a, t dbSNP:372277433
1478 1478 c, t dbSNP:766646882
1479 1479 a, g dbSNP:763290980
1498 1498 -, t dbSNP:772609811
1514 1514 a, c dbSNP:776762981
1515 1515 a, g dbSNP:200726800
1521 1521 a, g dbSNP:768608311
1525 1525 a, t dbSNP:760718889
1528 1528 a, g dbSNP:775261191
1530 1530 a, g dbSNP:794727842
1538 1538 c, t dbSNP:771932597
1561 1561 c, t dbSNP:745609590
1569 1569 c, t dbSNP:774159791
1570 1570 a, g dbSNP:147737629
1572 1572 a, g dbSNP:145677661
1575 1575 a, g dbSNP:200850870
1579 1579 a, g dbSNP:777398959
1580 1580 a, g dbSNP:755553945
1585 1585 c, t dbSNP:747589392
1587 1587 g, t dbSNP:188766510
1591 1591 a, g dbSNP:368746965
1606 1606 c, t dbSNP:750723018
1617 1617 c, t dbSNP:772705749
1625 1625 a, g dbSNP:1803946
1630 1630 c, t dbSNP:769374584
1636 1636 a, t dbSNP:747558018
1639 1639 a, g dbSNP:780621348
1642 1642 a, c dbSNP:758975398
1643 1643 a, g dbSNP:746365541
1646 1646 c, t dbSNP:779301267
1650 1650 a, c, t dbSNP:754123075
1651 1651 a, t dbSNP:764173488
1663 1663 a, g dbSNP:377604600
1664 1664 c, t dbSNP:752651149
1665 1665 a, g dbSNP:2229385
1669 1669 c, t dbSNP:766908649
1671 1671 c, g dbSNP:759605451
1674 1674 a, g dbSNP:774407830
1681 1681 c, g dbSNP:375164316
1682 1682 a, g dbSNP:762792688
1685 1685 c, t dbSNP:772936850
1689 1689 a, g dbSNP:769270504
1701 1701 c, t dbSNP:761525156
1702 1702 a, g dbSNP:776065390
1706 1706 a, c dbSNP:768136689
1709 1709 a, g dbSNP:201483594
1722 1722 c, g dbSNP:779354089
1729 1729 a, c dbSNP:199655896
1734 1734 a, c dbSNP:748358807
1735 1735 a, c, g dbSNP:144665233
1738 1738 c, g, t dbSNP:570658958
1740 1740 a, g dbSNP:372988725
1753 1753 c, t dbSNP:750355021
1764 1764 c, t dbSNP:764985110
1765 1765 a, c, g dbSNP:1141321
1767 1767 c, t dbSNP:753512817
1768 1768 a, g dbSNP:139765234
1773 1773 c, g dbSNP:760183775
1777 1777 c, t dbSNP:369902876
1784 1784 c, t dbSNP:766999822
1785 1785 a, g dbSNP:763417266
1792 1792 c, t dbSNP:773738310
1793 1793 g, t dbSNP:770086093
1795 1795 c, t dbSNP:748492928
1798 1798 a, g dbSNP:777001813
1799 1799 c, t dbSNP:150642856
1800 1800 a, g dbSNP:78150750
1809 1809 a, g dbSNP:780334099
1814 1814 a, c dbSNP:758475503
1815 1815 c, g dbSNP:541859735
1817 1817 a, g dbSNP:745828854
1819 1819 c, t dbSNP:778864344
1829 1829 a, g dbSNP:764316430
1833 1833 a, g dbSNP:753564352
1835 1835 a, g dbSNP:760537903
1840 1840 a, g dbSNP:756389945
1842 1842 g, t dbSNP:755824133
1849 1849 -, g dbSNP:766219320
1851 1851 a, g dbSNP:766368086
1859 1859 a, g dbSNP:764587313
1874 1874 c, t dbSNP:761076380
1888 1888 c, t dbSNP:775593146
1890 1890 a, g dbSNP:772269889
1903 1903 c, t dbSNP:759674071
1904 1904 a, g dbSNP:139323928
1907 1907 c, t dbSNP:770895512
1912 1912 a, g dbSNP:749261153
1921 1921 c, g dbSNP:777647799
1928 1928 a, g dbSNP:201138975
1932 1932 c, t dbSNP:140727018
1933 1933 a, g, t dbSNP:141829043
1947 1947 c, g dbSNP:148285323
1952 1952 g, t dbSNP:779657788
1956 1956 a, g dbSNP:545334525
1959 1959 a, t dbSNP:754422552
1962 1962 a, c, g dbSNP:9473556
1971 1971 a, g dbSNP:201888226
1979 1979 g, t dbSNP:756743196
1980 1980 g, t dbSNP:753123265
1983 1983 c, t dbSNP:767966332
1984 1984 a, g dbSNP:755256821
1987 1987 a, g dbSNP:751828125
1998 1998 c, t dbSNP:770978452
1999 1999 a, g dbSNP:766529896
2007 2007 c, t dbSNP:573727541
2008 2008 a, g dbSNP:368690932
2012 2012 a, g dbSNP:200996892
2016 2016 c, t dbSNP:765284825
2022 2022 -, ctt dbSNP:398123277
2035 2035 c, t dbSNP:201536536
2037 2037 a, g dbSNP:121918254
2041 2041 a, g dbSNP:768521956
2050 2050 a, g dbSNP:372486357
2056 2056 a, g dbSNP:775291072
2059 2059 a, g dbSNP:143023066
2064 2064 a, t dbSNP:745503019
2068 2068 g, t dbSNP:200055428
2070 2070 a, g dbSNP:778617168
2071 2071 g, t dbSNP:756798250
2076 2076 a, g dbSNP:569669261
2079 2079 a, g dbSNP:781501004
2094 2094 c, g dbSNP:747897332
2108 2108 c, t dbSNP:755382704
2112 2112 a, g dbSNP:751804284
2113 2113 a, g dbSNP:766721811
2114 2114 a, c dbSNP:758674900
2116 2116 -, gccc dbSNP:760692554
2122 2122 c, t dbSNP:1136145
2129 2129 c, t dbSNP:149460386
2133 2133 c, t dbSNP:541001298
2134 2134 a, c, g dbSNP:567485663
2139 2139 g, t dbSNP:370718165
2144 2144 c, t dbSNP:757366369
2161 2161 c, t dbSNP:137958217
2162 2162 a, g dbSNP:144146728
2165 2165 c, t dbSNP:760579526
2166 2166 g, t dbSNP:776430285
2167 2167 c, t dbSNP:767453976
2175 2175 a, g dbSNP:752634070
2179 2179 c, g dbSNP:759354207
2181 2181 a, g dbSNP:8589
2185 2185 a, g dbSNP:770423282
2192 2192 c, g, t dbSNP:772652266
2193 2193 a, g dbSNP:367850243
2195 2195 c, t dbSNP:373708083
2196 2196 a, g dbSNP:147715336
2203 2203 a, g dbSNP:147094927
2211 2211 c, t dbSNP:772442006
2216 2216 c, t dbSNP:201456803
2222 2222 a, g dbSNP:746167463
2225 2225 -, ctc dbSNP:565376922
2232 2232 g, t dbSNP:779331475
2241 2241 g, t dbSNP:757478500
2248 2248 g, t dbSNP:754072258
2250 2250 c, t dbSNP:777758903
2251 2251 a, g dbSNP:756225782
2252 2252 a, g dbSNP:752705830
2254 2254 c, t dbSNP:561197473
2257 2257 a, c dbSNP:759407117
2263 2263 a, t dbSNP:751462903
2269 2269 a, t dbSNP:140600746
2277 2277 c, g dbSNP:121918255
2284 2284 c, t dbSNP:772888575
2293 2293 a, g dbSNP:769217429
2300 2300 c, t dbSNP:375956047
2301 2301 g, t dbSNP:776176938
2307 2307 a, c dbSNP:188796821
2316 2316 a, g dbSNP:763572961
2318 2318 -, tggt dbSNP:748892245
2319 2319 c, g dbSNP:760079537
2320 2320 g, t dbSNP:121918252
2328 2328 a, g dbSNP:771224892
2330 2330 c, t dbSNP:749610593
2331 2331 a, g, t dbSNP:770205649
2332 2332 c, t dbSNP:748363752
2333 2333 a, g dbSNP:781466735
2338 2338 a, g dbSNP:755077681
2346 2346 a, t dbSNP:746950479
2349 2349 c, t dbSNP:779990936
2361 2361 a, g dbSNP:758134896
2362 2362 -, ggc dbSNP:781553746
2363 2363 -, t, tg dbSNP:747331003
2365 2365 -, cc dbSNP:778402274
2365 2365 a, c dbSNP:750300062
2366 2366 c, t dbSNP:201963242
2367 2367 -, aa dbSNP:758811462
2375 2375 a, g dbSNP:756952588
2376 2376 c, t dbSNP:753461919
2379 2379 c, g dbSNP:763629567
2388 2388 a, g dbSNP:372245965
2412 2412 c, t dbSNP:760186185
2420 2420 a, t dbSNP:774736275
2426 2426 c, t dbSNP:766839518
2427 2427 c, t dbSNP:763361965
2434 2434 c, t dbSNP:773680972
2453 2453 a, g dbSNP:14341
2457 2457 a, g dbSNP:199933641
2472 2472 a, t dbSNP:762165830
2474 2474 c, g dbSNP:199521080
2492 2492 c, t dbSNP:560764129
2496 2496 g, t dbSNP:548852414
2513 2513 a, g dbSNP:530424880
2524 2524 a, g dbSNP:113025987
2531 2531 a, c dbSNP:1803945
2549 2549 a, g dbSNP:192605776
2564 2564 c, t dbSNP:576032707
2597 2597 -, ca dbSNP:764175806
2616 2616 a, t dbSNP:76470114
2624 2624 -, a dbSNP:10713340
2639 2639 c, g dbSNP:764166500
2680 2680 a, c dbSNP:187747098
2701 2701 a, g dbSNP:182726681
2702 2702 c, t dbSNP:192552805
2703 2703 a, g dbSNP:761107295
2704 2704 a, g dbSNP:144168425
2726 2726 c, t dbSNP:113362290
2729 2729 c, t dbSNP:541575016
2738 2738 c, g dbSNP:1141333
2783 2783 -, gact dbSNP:576059123
2797 2797 c, g dbSNP:1141334
2833 2833 a, g dbSNP:776011450
2852 2852 a, t dbSNP:1141335
2874 2874 c, g dbSNP:558085628
2889 2889 a, g dbSNP:538293537
2896 2896 a, g dbSNP:569368840
2939 2939 a, g dbSNP:535324778
2952 2952 a, g dbSNP:187987243
2968 2968 a, c dbSNP:74854132
2981 2981 a, g dbSNP:111322712
2992 2992 c, t dbSNP:759680109
2998 2998 a, c, g dbSNP:190188528
3003 3003 a, g dbSNP:11757098
3023 3023 a, g dbSNP:552751982
3050 3050 c, t dbSNP:534290000
3104 3104 c, t dbSNP:186205803
3121 3121 c, g dbSNP:548813379
3220 3220 g, t dbSNP:377179859
3232 3232 a, t dbSNP:530299655
3249 3249 a, g dbSNP:139283545
3257 3257 c, t dbSNP:771269097
3303 3303 -, a dbSNP:777905388
3318 3318 a, g dbSNP:563222609
3332 3332 c, t dbSNP:761072490
3339 3339 c, t dbSNP:773671452
3342 3342 g, t dbSNP:772592770
3367 3367 a, g dbSNP:748775102
3435 3435 -, gat dbSNP:752978672
3467 3467 a, t dbSNP:779436906
3505 3505 -, cag dbSNP:756006397
3516 3516 a, g dbSNP:146257268
3530 3530 -, a dbSNP:71777574
3563 3563 a, g dbSNP:566780363
3598 3598 a, g dbSNP:531378878
3606 3606 a, g dbSNP:563837981
3607 3607 c, t dbSNP:745494100
3632 3632 a, g dbSNP:545448500
3648 3648 a, g dbSNP:571818982
3668 3668 a, g dbSNP:559882082
3675 3675 c, t dbSNP:781039564
3742 3742 c, t dbSNP:541645779
3749 3749 a, g dbSNP:9381784
3775 3775 c, t dbSNP:769328040
3780 3780 g, t dbSNP:747868663

Target ORF information:

RefSeq Version XM_005249143
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens methylmalonyl CoA mutase (MUT), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005249143

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTTAAGAG CTAAGAATCA GCTTTTTTTA CTTTCACCTC ATTACCTGAG GCAGGTAAAA 
GAATCATCAG GCTCCAGGCT CATACAGCAA CGACTTCTAC ACCAGCAACA GCCCCTTCAC
CCAGAATGGG CTGCCCTGGC TAAAAAGCAG CTGAAAGGCA AAAACCCAGA AGACCTAATA
TGGCACACCC CGGAAGGGAT CTCTATAAAA CCCTTGTATT CCAAGAGAGA TACTATGGAC
TTACCTGAAG AACTTCCAGG AGTGAAGCCA TTCACACGTG GACCATATCC TACCATGTAT
ACCTTTAGGC CCTGGACCAT CCGCCAGTAT GCTGGTTTTA GTACTGTGGA AGAAAGCAAT
AAGTTCTATA AGGACAACAT TAAGGCTGGT CAGCAGGGAT TATCAGTTGC CTTTGATCTG
GCGACACATC GTGGCTATGA TTCAGACAAC CCTCGAGTTC GTGGTGATGT TGGAATGGCT
GGAGTTGCTA TTGACACTGT GGAAGATACC AAAATTCTTT TTGATGGAAT TCCTTTAGAA
AAAATGTCAG TTTCCATGAC TATGAATGGA GCAGTTATTC CAGTTCTTGC AAATTTTATA
GTAACTGGAG AAGAACAAGG TGTACCTAAA GAGAAGCTTA CTGGTACCAT CCAAAATGAT
ATACTAAAGG AATTTATGGT TCGAAATACA TACATTTTTC CTCCAGAACC ATCCATGAAA
ATTATTGCTG ACATATTTGA ATATACAGCA AAGCACATGC CAAAATTTAA TTCAATTTCA
ATTAGTGGAT ACCATATGCA GGAAGCAGGG GCTGATGCCA TTCTGGAGCT GGCCTATACT
TTAGCAGATG GATTGGAGTA CTCTAGAACT GGACTCCAGG CTGGCCTGAC AATTGATGAA
TTTGCACCAA GGTTGTCTTT CTTCTGGGGA ATTGGAATGA ATTTCTATAT GGAAATAGCA
AAGATGAGAG CTGGTAGAAG ACTCTGGGCT CACTTAATAG AGAAAATGTT TCAGCCTAAA
AACTCAAAAT CTCTTCTTCT AAGAGCACAC TGTCAGACAT CTGGATGGTC ACTTACTGAG
CAGGATCCCT ACAATAATAT TGTCCGTACT GCAATAGAAG CAATGGCAGC AGTATTTGGA
GGGACTCAGT CTTTGCACAC AAATTCTTTT GATGAAGCTT TGGGTTTGCC AACTGTGAAA
AGTGCTCGAA TTGCCAGGAA CACACAAATC ATCATTCAAG AAGAATCTGG GATTCCCAAA
GTGGCTGATC CTTGGGGAGG TTCTTACATG ATGGAATGTC TCACAAATGA TGTTTATGAT
GCTGCTTTAA AGCTCATTAA TGAAATTGAA GAAATGGGTG GAATGGCCAA AGCTGTAGCT
GAGGGAATAC CTAAACTTCG AATTGAAGAA TGTGCTGCCC GAAGACAAGC TAGAATAGAT
TCTGGTTCTG AAGTAATTGT TGGAGTAAAT AAGTACCAGT TGGAAAAAGA AGACGCTGTA
GAAGTTCTGG CAATTGATAA TACTTCAGTG CGAAACAGGC AGATTGAAAA ACTTAAGAAG
ATCAAATCCA GCAGGGATCA AGCTTTGGCT GAACGTTGTC TTGCTGCACT AACCGAATGT
GCTGCTAGCG GAGATGGAAA TATCCTGGCT CTTGCAGTGG ATGCATCTCG GGCAAGATGT
ACAGTGGGAG AAATCACAGA TGCCCTGAAA AAGGTATTTG GTGAACATAA AGCGAATGAT
CGAATGGTGA GTGGAGCATA TCGCCAGGAA TTTGGAGAAA GTAAAGAGAT AACATCTGCT
ATCAAGAGGG TTCATAAATT CATGGAACGT GAAGGTCGCA GACCTCGTCT TCTTGTAGCA
AAAATGGGAC AAGATGGCCA TGACAGAGGA GCAAAAGTTA TTGCTACAGG ATTTGCTGAT
CTTGGTTTTG ATGTGGACAT AGGCCCTCTT TTCCAGACTC CTCGTGAAGT GGCCCAGCAG
GCTGTGGATG CGGATGTGCA TGCTGTGGGC ATAAGCACCC TCGCTGCTGG TCATAAAACC
CTAGTTCCTG AACTCATCAA AGAACTTAAC TCCCTTGGAC GGCCAGATAT TCTTGTCATG
TGTGGAGGGG TGATACCACC TCAGGATTAT GAATTTCTGT TTGAAGTTGG TGTTTCCAAT
GTATTTGGTC CTGGGACTCG AATTCCAAAG GCTGCCGTTC AGGTGCTTGA TGATATTGAG
AAGTGTTTGG AAAAGAAGCA GCAATCTGTA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.