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MUT cDNA ORF clone, Homo sapiens (human)

Gene Symbol MUT
Entrez Gene ID 4594
Full Name methylmalonyl CoA mutase
Synonyms MCM
General protein information
Preferred Names
methylmalonyl-CoA mutase, mitochondrial
Names
methylmalonyl-CoA mutase, mitochondrial
methylmalonyl-CoA isomerase
methylmalonyl Coenzyme A mutase
NP_000246.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p12.3

Summary This gene encodes the mitochondrial enzyme methylmalonyl Coenzyme A mutase. In humans, the product of this gene is a vitamin B12-dependent enzyme which catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA, while in other species this enzyme may have different functions. Mutations in this gene may lead to various types of methylmalonic aciduria. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

609058

Disorder Html: Methylmalonic aciduria, mut(0) type, 251000 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000255 NP_000246 methylmalonyl-CoA mutase, mitochondrial precursor
XM_005249143 XP_005249200 methylmalonyl-CoA mutase, mitochondrial isoform X1



Homo sapiens (human) MUT NP_000246.2
Pan troglodytes (chimpanzee) MUT XP_001146876.1
Macaca mulatta (Rhesus monkey) MUT XP_001104531.1
Canis lupus familiaris (dog) MUT XP_532164.3
Bos taurus (cattle) MUT NP_776364.2
Mus musculus (house mouse) Mut NP_032676.2
Rattus norvegicus (Norway rat) Mut XP_006244706.1
Gallus gallus (chicken) MUT XP_004935974.1
Danio rerio (zebrafish) mut NP_001092696.1
Caenorhabditis elegans mmcm-1 NP_497786.2
Xenopus (Silurana) tropicalis (western clawed frog) mut XP_004915010.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MUT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MUT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu25184 NM_000255 Homo sapiens methylmalonyl CoA mutase (MUT), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $265.30
$379.00
OHu25184 XM_005249143 PREDICTED: Homo sapiens methylmalonyl CoA mutase (MUT), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25184
Clone ID Related Accession (Same CDS sequence) NM_000255 , XM_005249143
Accession Version NM_000255.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 04-AUG-2015
Organism Homo sapiens (human)
Product methylmalonyl-CoA mutase, mitochondrial precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA777441.1, AK292568.1, AK312611.1 and AL590668.5. This sequence is a reference standard in the RefSeqGene project. On May 13, 2010 this sequence version replaced gi:156105688. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292568.1, M65131.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTTAAGAG CTAAGAATCA GCTTTTTTTA CTTTCACCTC ATTACCTGAG GCAGGTAAAA 
GAATCATCAG GCTCCAGGCT CATACAGCAA CGACTTCTAC ACCAGCAACA GCCCCTTCAC
CCAGAATGGG CTGCCCTGGC TAAAAAGCAG CTGAAAGGCA AAAACCCAGA AGACCTAATA
TGGCACACCC CGGAAGGGAT CTCTATAAAA CCCTTGTATT CCAAGAGAGA TACTATGGAC
TTACCTGAAG AACTTCCAGG AGTGAAGCCA TTCACACGTG GACCATATCC TACCATGTAT
ACCTTTAGGC CCTGGACCAT CCGCCAGTAT GCTGGTTTTA GTACTGTGGA AGAAAGCAAT
AAGTTCTATA AGGACAACAT TAAGGCTGGT CAGCAGGGAT TATCAGTTGC CTTTGATCTG
GCGACACATC GTGGCTATGA TTCAGACAAC CCTCGAGTTC GTGGTGATGT TGGAATGGCT
GGAGTTGCTA TTGACACTGT GGAAGATACC AAAATTCTTT TTGATGGAAT TCCTTTAGAA
AAAATGTCAG TTTCCATGAC TATGAATGGA GCAGTTATTC CAGTTCTTGC AAATTTTATA
GTAACTGGAG AAGAACAAGG TGTACCTAAA GAGAAGCTTA CTGGTACCAT CCAAAATGAT
ATACTAAAGG AATTTATGGT TCGAAATACA TACATTTTTC CTCCAGAACC ATCCATGAAA
ATTATTGCTG ACATATTTGA ATATACAGCA AAGCACATGC CAAAATTTAA TTCAATTTCA
ATTAGTGGAT ACCATATGCA GGAAGCAGGG GCTGATGCCA TTCTGGAGCT GGCCTATACT
TTAGCAGATG GATTGGAGTA CTCTAGAACT GGACTCCAGG CTGGCCTGAC AATTGATGAA
TTTGCACCAA GGTTGTCTTT CTTCTGGGGA ATTGGAATGA ATTTCTATAT GGAAATAGCA
AAGATGAGAG CTGGTAGAAG ACTCTGGGCT CACTTAATAG AGAAAATGTT TCAGCCTAAA
AACTCAAAAT CTCTTCTTCT AAGAGCACAC TGTCAGACAT CTGGATGGTC ACTTACTGAG
CAGGATCCCT ACAATAATAT TGTCCGTACT GCAATAGAAG CAATGGCAGC AGTATTTGGA
GGGACTCAGT CTTTGCACAC AAATTCTTTT GATGAAGCTT TGGGTTTGCC AACTGTGAAA
AGTGCTCGAA TTGCCAGGAA CACACAAATC ATCATTCAAG AAGAATCTGG GATTCCCAAA
GTGGCTGATC CTTGGGGAGG TTCTTACATG ATGGAATGTC TCACAAATGA TGTTTATGAT
GCTGCTTTAA AGCTCATTAA TGAAATTGAA GAAATGGGTG GAATGGCCAA AGCTGTAGCT
GAGGGAATAC CTAAACTTCG AATTGAAGAA TGTGCTGCCC GAAGACAAGC TAGAATAGAT
TCTGGTTCTG AAGTAATTGT TGGAGTAAAT AAGTACCAGT TGGAAAAAGA AGACGCTGTA
GAAGTTCTGG CAATTGATAA TACTTCAGTG CGAAACAGGC AGATTGAAAA ACTTAAGAAG
ATCAAATCCA GCAGGGATCA AGCTTTGGCT GAACGTTGTC TTGCTGCACT AACCGAATGT
GCTGCTAGCG GAGATGGAAA TATCCTGGCT CTTGCAGTGG ATGCATCTCG GGCAAGATGT
ACAGTGGGAG AAATCACAGA TGCCCTGAAA AAGGTATTTG GTGAACATAA AGCGAATGAT
CGAATGGTGA GTGGAGCATA TCGCCAGGAA TTTGGAGAAA GTAAAGAGAT AACATCTGCT
ATCAAGAGGG TTCATAAATT CATGGAACGT GAAGGTCGCA GACCTCGTCT TCTTGTAGCA
AAAATGGGAC AAGATGGCCA TGACAGAGGA GCAAAAGTTA TTGCTACAGG ATTTGCTGAT
CTTGGTTTTG ATGTGGACAT AGGCCCTCTT TTCCAGACTC CTCGTGAAGT GGCCCAGCAG
GCTGTGGATG CGGATGTGCA TGCTGTGGGC ATAAGCACCC TCGCTGCTGG TCATAAAACC
CTAGTTCCTG AACTCATCAA AGAACTTAAC TCCCTTGGAC GGCCAGATAT TCTTGTCATG
TGTGGAGGGG TGATACCACC TCAGGATTAT GAATTTCTGT TTGAAGTTGG TGTTTCCAAT
GTATTTGGTC CTGGGACTCG AATTCCAAAG GCTGCCGTTC AGGTGCTTGA TGATATTGAG
AAGTGTTTGG AAAAGAAGCA GCAATCTGTA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000246.2
CDS266..2518
Misc Feature(1)89..91(+)
Misc Feature(2)383..1987(+)
Misc Feature(3)386..2497(+)
Misc Feature(4)530..532(+)
Misc Feature(5)551..1453(+)
Misc Feature(6)551..1414(+)
Misc Feature(7)593..1453(+)
Misc Feature(8)899..901(+)
Misc Feature(9)1268..1270(+)
Misc Feature(10)1292..1294(+)
Misc Feature(11)1706..1708(+)
Misc Feature(12)2048..2050(+)
Misc Feature(13)2069..2071(+)
Misc Feature(14)2111..2476(+)
Misc Feature(15)2135..2458(+)
Misc Feature(16)2144..2146(+)
Exon (1)1..226
Gene:MUT
Gene Synonym:
Exon (2)227..650
Gene:MUT
Gene Synonym:
Exon (3)651..1018
Gene:MUT
Gene Synonym:
Exon (4)1019..1176
Gene:MUT
Gene Synonym:
Exon (5)1177..1348
Gene:MUT
Gene Synonym:
Exon (6)1349..1597
Gene:MUT
Gene Synonym:
Exon (7)1598..1709
Gene:MUT
Gene Synonym:
Exon (8)1710..1825
Gene:MUT
Gene Synonym:
Exon (9)1826..1941
Gene:MUT
Gene Synonym:
Exon (10)1942..2073
Gene:MUT
Gene Synonym:
Exon (11)2074..2221
Gene:MUT
Gene Synonym:
Exon (12)2222..2389
Gene:MUT
Gene Synonym:
Exon (13)2390..3886
Gene:MUT
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000255
Organism Homo sapiens (human)
Definition Homo sapiens methylmalonyl CoA mutase (MUT), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000255

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTTAAGAG CTAAGAATCA GCTTTTTTTA CTTTCACCTC ATTACCTGAG GCAGGTAAAA 
GAATCATCAG GCTCCAGGCT CATACAGCAA CGACTTCTAC ACCAGCAACA GCCCCTTCAC
CCAGAATGGG CTGCCCTGGC TAAAAAGCAG CTGAAAGGCA AAAACCCAGA AGACCTAATA
TGGCACACCC CGGAAGGGAT CTCTATAAAA CCCTTGTATT CCAAGAGAGA TACTATGGAC
TTACCTGAAG AACTTCCAGG AGTGAAGCCA TTCACACGTG GACCATATCC TACCATGTAT
ACCTTTAGGC CCTGGACCAT CCGCCAGTAT GCTGGTTTTA GTACTGTGGA AGAAAGCAAT
AAGTTCTATA AGGACAACAT TAAGGCTGGT CAGCAGGGAT TATCAGTTGC CTTTGATCTG
GCGACACATC GTGGCTATGA TTCAGACAAC CCTCGAGTTC GTGGTGATGT TGGAATGGCT
GGAGTTGCTA TTGACACTGT GGAAGATACC AAAATTCTTT TTGATGGAAT TCCTTTAGAA
AAAATGTCAG TTTCCATGAC TATGAATGGA GCAGTTATTC CAGTTCTTGC AAATTTTATA
GTAACTGGAG AAGAACAAGG TGTACCTAAA GAGAAGCTTA CTGGTACCAT CCAAAATGAT
ATACTAAAGG AATTTATGGT TCGAAATACA TACATTTTTC CTCCAGAACC ATCCATGAAA
ATTATTGCTG ACATATTTGA ATATACAGCA AAGCACATGC CAAAATTTAA TTCAATTTCA
ATTAGTGGAT ACCATATGCA GGAAGCAGGG GCTGATGCCA TTCTGGAGCT GGCCTATACT
TTAGCAGATG GATTGGAGTA CTCTAGAACT GGACTCCAGG CTGGCCTGAC AATTGATGAA
TTTGCACCAA GGTTGTCTTT CTTCTGGGGA ATTGGAATGA ATTTCTATAT GGAAATAGCA
AAGATGAGAG CTGGTAGAAG ACTCTGGGCT CACTTAATAG AGAAAATGTT TCAGCCTAAA
AACTCAAAAT CTCTTCTTCT AAGAGCACAC TGTCAGACAT CTGGATGGTC ACTTACTGAG
CAGGATCCCT ACAATAATAT TGTCCGTACT GCAATAGAAG CAATGGCAGC AGTATTTGGA
GGGACTCAGT CTTTGCACAC AAATTCTTTT GATGAAGCTT TGGGTTTGCC AACTGTGAAA
AGTGCTCGAA TTGCCAGGAA CACACAAATC ATCATTCAAG AAGAATCTGG GATTCCCAAA
GTGGCTGATC CTTGGGGAGG TTCTTACATG ATGGAATGTC TCACAAATGA TGTTTATGAT
GCTGCTTTAA AGCTCATTAA TGAAATTGAA GAAATGGGTG GAATGGCCAA AGCTGTAGCT
GAGGGAATAC CTAAACTTCG AATTGAAGAA TGTGCTGCCC GAAGACAAGC TAGAATAGAT
TCTGGTTCTG AAGTAATTGT TGGAGTAAAT AAGTACCAGT TGGAAAAAGA AGACGCTGTA
GAAGTTCTGG CAATTGATAA TACTTCAGTG CGAAACAGGC AGATTGAAAA ACTTAAGAAG
ATCAAATCCA GCAGGGATCA AGCTTTGGCT GAACGTTGTC TTGCTGCACT AACCGAATGT
GCTGCTAGCG GAGATGGAAA TATCCTGGCT CTTGCAGTGG ATGCATCTCG GGCAAGATGT
ACAGTGGGAG AAATCACAGA TGCCCTGAAA AAGGTATTTG GTGAACATAA AGCGAATGAT
CGAATGGTGA GTGGAGCATA TCGCCAGGAA TTTGGAGAAA GTAAAGAGAT AACATCTGCT
ATCAAGAGGG TTCATAAATT CATGGAACGT GAAGGTCGCA GACCTCGTCT TCTTGTAGCA
AAAATGGGAC AAGATGGCCA TGACAGAGGA GCAAAAGTTA TTGCTACAGG ATTTGCTGAT
CTTGGTTTTG ATGTGGACAT AGGCCCTCTT TTCCAGACTC CTCGTGAAGT GGCCCAGCAG
GCTGTGGATG CGGATGTGCA TGCTGTGGGC ATAAGCACCC TCGCTGCTGG TCATAAAACC
CTAGTTCCTG AACTCATCAA AGAACTTAAC TCCCTTGGAC GGCCAGATAT TCTTGTCATG
TGTGGAGGGG TGATACCACC TCAGGATTAT GAATTTCTGT TTGAAGTTGG TGTTTCCAAT
GTATTTGGTC CTGGGACTCG AATTCCAAAG GCTGCCGTTC AGGTGCTTGA TGATATTGAG
AAGTGTTTGG AAAAGAAGCA GCAATCTGTA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25184
Clone ID Related Accession (Same CDS sequence) NM_000255 , XM_005249143
Accession Version XM_005249143.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product methylmalonyl-CoA mutase, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007592.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced gi:530382072. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTTAAGAG CTAAGAATCA GCTTTTTTTA CTTTCACCTC ATTACCTGAG GCAGGTAAAA 
GAATCATCAG GCTCCAGGCT CATACAGCAA CGACTTCTAC ACCAGCAACA GCCCCTTCAC
CCAGAATGGG CTGCCCTGGC TAAAAAGCAG CTGAAAGGCA AAAACCCAGA AGACCTAATA
TGGCACACCC CGGAAGGGAT CTCTATAAAA CCCTTGTATT CCAAGAGAGA TACTATGGAC
TTACCTGAAG AACTTCCAGG AGTGAAGCCA TTCACACGTG GACCATATCC TACCATGTAT
ACCTTTAGGC CCTGGACCAT CCGCCAGTAT GCTGGTTTTA GTACTGTGGA AGAAAGCAAT
AAGTTCTATA AGGACAACAT TAAGGCTGGT CAGCAGGGAT TATCAGTTGC CTTTGATCTG
GCGACACATC GTGGCTATGA TTCAGACAAC CCTCGAGTTC GTGGTGATGT TGGAATGGCT
GGAGTTGCTA TTGACACTGT GGAAGATACC AAAATTCTTT TTGATGGAAT TCCTTTAGAA
AAAATGTCAG TTTCCATGAC TATGAATGGA GCAGTTATTC CAGTTCTTGC AAATTTTATA
GTAACTGGAG AAGAACAAGG TGTACCTAAA GAGAAGCTTA CTGGTACCAT CCAAAATGAT
ATACTAAAGG AATTTATGGT TCGAAATACA TACATTTTTC CTCCAGAACC ATCCATGAAA
ATTATTGCTG ACATATTTGA ATATACAGCA AAGCACATGC CAAAATTTAA TTCAATTTCA
ATTAGTGGAT ACCATATGCA GGAAGCAGGG GCTGATGCCA TTCTGGAGCT GGCCTATACT
TTAGCAGATG GATTGGAGTA CTCTAGAACT GGACTCCAGG CTGGCCTGAC AATTGATGAA
TTTGCACCAA GGTTGTCTTT CTTCTGGGGA ATTGGAATGA ATTTCTATAT GGAAATAGCA
AAGATGAGAG CTGGTAGAAG ACTCTGGGCT CACTTAATAG AGAAAATGTT TCAGCCTAAA
AACTCAAAAT CTCTTCTTCT AAGAGCACAC TGTCAGACAT CTGGATGGTC ACTTACTGAG
CAGGATCCCT ACAATAATAT TGTCCGTACT GCAATAGAAG CAATGGCAGC AGTATTTGGA
GGGACTCAGT CTTTGCACAC AAATTCTTTT GATGAAGCTT TGGGTTTGCC AACTGTGAAA
AGTGCTCGAA TTGCCAGGAA CACACAAATC ATCATTCAAG AAGAATCTGG GATTCCCAAA
GTGGCTGATC CTTGGGGAGG TTCTTACATG ATGGAATGTC TCACAAATGA TGTTTATGAT
GCTGCTTTAA AGCTCATTAA TGAAATTGAA GAAATGGGTG GAATGGCCAA AGCTGTAGCT
GAGGGAATAC CTAAACTTCG AATTGAAGAA TGTGCTGCCC GAAGACAAGC TAGAATAGAT
TCTGGTTCTG AAGTAATTGT TGGAGTAAAT AAGTACCAGT TGGAAAAAGA AGACGCTGTA
GAAGTTCTGG CAATTGATAA TACTTCAGTG CGAAACAGGC AGATTGAAAA ACTTAAGAAG
ATCAAATCCA GCAGGGATCA AGCTTTGGCT GAACGTTGTC TTGCTGCACT AACCGAATGT
GCTGCTAGCG GAGATGGAAA TATCCTGGCT CTTGCAGTGG ATGCATCTCG GGCAAGATGT
ACAGTGGGAG AAATCACAGA TGCCCTGAAA AAGGTATTTG GTGAACATAA AGCGAATGAT
CGAATGGTGA GTGGAGCATA TCGCCAGGAA TTTGGAGAAA GTAAAGAGAT AACATCTGCT
ATCAAGAGGG TTCATAAATT CATGGAACGT GAAGGTCGCA GACCTCGTCT TCTTGTAGCA
AAAATGGGAC AAGATGGCCA TGACAGAGGA GCAAAAGTTA TTGCTACAGG ATTTGCTGAT
CTTGGTTTTG ATGTGGACAT AGGCCCTCTT TTCCAGACTC CTCGTGAAGT GGCCCAGCAG
GCTGTGGATG CGGATGTGCA TGCTGTGGGC ATAAGCACCC TCGCTGCTGG TCATAAAACC
CTAGTTCCTG AACTCATCAA AGAACTTAAC TCCCTTGGAC GGCCAGATAT TCTTGTCATG
TGTGGAGGGG TGATACCACC TCAGGATTAT GAATTTCTGT TTGAAGTTGG TGTTTCCAAT
GTATTTGGTC CTGGGACTCG AATTCCAAAG GCTGCCGTTC AGGTGCTTGA TGATATTGAG
AAGTGTTTGG AAAAGAAGCA GCAATCTGTA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005249200.1
CDS171..2423
Misc Feature(1)288..1892(+)
Misc Feature(2)291..2402(+)
Misc Feature(3)456..1358(+)
Misc Feature(4)456..1319(+)
Misc Feature(5)498..1358(+)
Misc Feature(6)2016..2381(+)
Misc Feature(7)2040..2363(+)
Misc Feature(8)2049..2051(+)
Translation

Target ORF information:

RefSeq Version XM_005249143
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens methylmalonyl CoA mutase (MUT), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005249143

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTTAAGAG CTAAGAATCA GCTTTTTTTA CTTTCACCTC ATTACCTGAG GCAGGTAAAA 
GAATCATCAG GCTCCAGGCT CATACAGCAA CGACTTCTAC ACCAGCAACA GCCCCTTCAC
CCAGAATGGG CTGCCCTGGC TAAAAAGCAG CTGAAAGGCA AAAACCCAGA AGACCTAATA
TGGCACACCC CGGAAGGGAT CTCTATAAAA CCCTTGTATT CCAAGAGAGA TACTATGGAC
TTACCTGAAG AACTTCCAGG AGTGAAGCCA TTCACACGTG GACCATATCC TACCATGTAT
ACCTTTAGGC CCTGGACCAT CCGCCAGTAT GCTGGTTTTA GTACTGTGGA AGAAAGCAAT
AAGTTCTATA AGGACAACAT TAAGGCTGGT CAGCAGGGAT TATCAGTTGC CTTTGATCTG
GCGACACATC GTGGCTATGA TTCAGACAAC CCTCGAGTTC GTGGTGATGT TGGAATGGCT
GGAGTTGCTA TTGACACTGT GGAAGATACC AAAATTCTTT TTGATGGAAT TCCTTTAGAA
AAAATGTCAG TTTCCATGAC TATGAATGGA GCAGTTATTC CAGTTCTTGC AAATTTTATA
GTAACTGGAG AAGAACAAGG TGTACCTAAA GAGAAGCTTA CTGGTACCAT CCAAAATGAT
ATACTAAAGG AATTTATGGT TCGAAATACA TACATTTTTC CTCCAGAACC ATCCATGAAA
ATTATTGCTG ACATATTTGA ATATACAGCA AAGCACATGC CAAAATTTAA TTCAATTTCA
ATTAGTGGAT ACCATATGCA GGAAGCAGGG GCTGATGCCA TTCTGGAGCT GGCCTATACT
TTAGCAGATG GATTGGAGTA CTCTAGAACT GGACTCCAGG CTGGCCTGAC AATTGATGAA
TTTGCACCAA GGTTGTCTTT CTTCTGGGGA ATTGGAATGA ATTTCTATAT GGAAATAGCA
AAGATGAGAG CTGGTAGAAG ACTCTGGGCT CACTTAATAG AGAAAATGTT TCAGCCTAAA
AACTCAAAAT CTCTTCTTCT AAGAGCACAC TGTCAGACAT CTGGATGGTC ACTTACTGAG
CAGGATCCCT ACAATAATAT TGTCCGTACT GCAATAGAAG CAATGGCAGC AGTATTTGGA
GGGACTCAGT CTTTGCACAC AAATTCTTTT GATGAAGCTT TGGGTTTGCC AACTGTGAAA
AGTGCTCGAA TTGCCAGGAA CACACAAATC ATCATTCAAG AAGAATCTGG GATTCCCAAA
GTGGCTGATC CTTGGGGAGG TTCTTACATG ATGGAATGTC TCACAAATGA TGTTTATGAT
GCTGCTTTAA AGCTCATTAA TGAAATTGAA GAAATGGGTG GAATGGCCAA AGCTGTAGCT
GAGGGAATAC CTAAACTTCG AATTGAAGAA TGTGCTGCCC GAAGACAAGC TAGAATAGAT
TCTGGTTCTG AAGTAATTGT TGGAGTAAAT AAGTACCAGT TGGAAAAAGA AGACGCTGTA
GAAGTTCTGG CAATTGATAA TACTTCAGTG CGAAACAGGC AGATTGAAAA ACTTAAGAAG
ATCAAATCCA GCAGGGATCA AGCTTTGGCT GAACGTTGTC TTGCTGCACT AACCGAATGT
GCTGCTAGCG GAGATGGAAA TATCCTGGCT CTTGCAGTGG ATGCATCTCG GGCAAGATGT
ACAGTGGGAG AAATCACAGA TGCCCTGAAA AAGGTATTTG GTGAACATAA AGCGAATGAT
CGAATGGTGA GTGGAGCATA TCGCCAGGAA TTTGGAGAAA GTAAAGAGAT AACATCTGCT
ATCAAGAGGG TTCATAAATT CATGGAACGT GAAGGTCGCA GACCTCGTCT TCTTGTAGCA
AAAATGGGAC AAGATGGCCA TGACAGAGGA GCAAAAGTTA TTGCTACAGG ATTTGCTGAT
CTTGGTTTTG ATGTGGACAT AGGCCCTCTT TTCCAGACTC CTCGTGAAGT GGCCCAGCAG
GCTGTGGATG CGGATGTGCA TGCTGTGGGC ATAAGCACCC TCGCTGCTGG TCATAAAACC
CTAGTTCCTG AACTCATCAA AGAACTTAAC TCCCTTGGAC GGCCAGATAT TCTTGTCATG
TGTGGAGGGG TGATACCACC TCAGGATTAT GAATTTCTGT TTGAAGTTGG TGTTTCCAAT
GTATTTGGTC CTGGGACTCG AATTCCAAAG GCTGCCGTTC AGGTGCTTGA TGATATTGAG
AAGTGTTTGG AAAAGAAGCA GCAATCTGTA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Functional characterization and categorization of missense mutations that cause methylmalonyl-CoA mutase (MUT) deficiency
Hum. Mutat. 35 (12), 1449-1458 (2014)
Forny P, Froese DS, Suormala T, Yue WW and Baumgartner MR.


book

Noninvasive prenatal diagnosis in a fetus at risk for methylmalonic acidemia
Genet. Med. 16 (7), 564-567 (2014)
Gu W, Koh W, Blumenfeld YJ, El-Sayed YY, Hudgins L, Hintz SR and Quake SR.


book

Asymptomatic methylmalonic acidemia in a homozygous MUT mutation (p.P86L)
Pediatr Int 55 (6), E156-E158 (2013)
Underhill HR, Hahn SH, Hale SL and Merritt JL 2nd.


book

A switch III motif relays signaling between a B12 enzyme and its G-protein chaperone
Nat. Chem. Biol. 9 (9), 535-539 (2013)
Lofgren M, Padovani D, Koutmos M and Banerjee R.


book

Methylmalonic Acidemia
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Manoli,I. and Venditti,C.P.


book

Dystonia Overview
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Klein,C., Marras,C. and Munchau,A.


book

The Organic Acidemias: An Overview
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Seashore,M.R.


book

Phenotype of disease in three patients with identical mutations in methylmalonyl CoA mutase
Hum. Genet. 89 (3), 259-264 (1992)
Crane AM, Martin LS, Valle D and Ledley FD.


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Cloning and expression of a mutant methylmalonyl coenzyme A mutase with altered cobalamin affinity that causes mut- methylmalonic aciduria
J. Clin. Invest. 89 (2), 385-391 (1992)
Crane AM, Jansen R, Andrews ER and Ledley FD.


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Intracellular localization of hepatic propionyl-CoA carboxylase and methylmalonyl-CoA mutase in humans and normal and vitamin B12 deficient rats
Br. J. Haematol. 31 (4), 501-513 (1975)
Frenkel,E.P. and Kitchens,R.L.


 
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