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MYL1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MYL1
Entrez Gene ID 4632
Full Name myosin, light chain 1, alkali; skeletal, fast
Synonyms MLC1F, MLC3F
General protein information
Preferred Names
myosin light chain 1/3, skeletal muscle isoform
Names
myosin light chain 1/3, skeletal muscle isoform
MLC1/MLC3
MLC1F/MLC3F
A1 catalytic
A2 catalytic
myosin light chain A1/A2
myosin light chain alkali 1/2
myosin, light polypeptide 1, alkali; skeletal, fast
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2q34

Summary Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

160780


mRNA and Protein(s)

mRNA Protein Name
NM_079422 NP_524146 myosin light chain 1/3, skeletal muscle isoform isoform 3f
NM_079420 NP_524144 myosin light chain 1/3, skeletal muscle isoform isoform 1f



Homo sapiens (human) MYL1 NP_524144.1
Pan troglodytes (chimpanzee) MYL1 XP_516064.2
Canis lupus familiaris (dog) MYL1 XP_005640670.1
Bos taurus (cattle) MYL1 NP_001073046.1
Mus musculus (house mouse) Myl1 NP_067260.1
Rattus norvegicus (Norway rat) Myl1 NP_001071124.1
Gallus gallus (chicken) MYL1 NP_001038097.1
Danio rerio (zebrafish) myl1 NP_956294.1
Danio rerio (zebrafish) mylz3 NP_571694.1
Xenopus (Silurana) tropicalis (western clawed frog) myl1 NP_988954.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MYL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu13487
NM_079422 Homo sapiens myosin, light chain 1, alkali; skeletal, fast (MYL1), transcript variant 3f, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu13342 NM_079420 Homo sapiens myosin, light chain 1, alkali; skeletal, fast (MYL1), transcript variant 1f, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu13487
Clone ID Related Accession (Same CDS sequence) NM_079422
Accession Version NM_079422.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 453bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 28-APR-2015
Organism Homo sapiens (human)
Product myosin light chain 1/3, skeletal muscle isoform isoform 3f
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005318.1 and AA705167.1. On Oct 4, 2006 this sequence version replaced gi:17986274. Transcript Variant: This variant (3f) encodes a protein that is 44 aa shorter than isoform 1f. Transcript variants 3f and 1f differ in their first two exons. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005318.1, BX436371.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162946 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
ATGTCCTTCA GTGCTGACCA GATTGCTGAA TTCAAGGAGG CATTTCTCCT GTTTGACAGA 
ACAGGTGATT CCAAGATCAC CTTAAGCCAG GTCGGTGATG TCCTTCGAGC TCTGGGCACA
AATCCCACCA ATGCAGAGGT CAGGAAAGTT CTGGGAAACC CCAGCAATGA AGAGCTGAAT
GCCAAGAAAA TTGAGTTTGA ACAATTTCTG CCTATGATGC AAGCCATTTC CAACAACAAG
GACCAGGCCA CCTATGAAGA CTTTGTTGAG GGTCTGCGTG TCTTTGACAA GGAAGGCAAT
GGCACAGTCA TGGGTGCTGA ACTCCGCCAT GTTCTAGCCA CCCTGGGTGA AAAGATGAAA
GAGGAAGAAG TGGAAGCCCT GATGGCAGGT CAAGAAGACT CCAATGGCTG CATCAACTAC
GAAGCTTTTG TCAAGCACAT CATGTCTATC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_524146.1
CDS72..524
Misc Feature(1)81..518(+)
Misc Feature(2)99..293(+)
Misc Feature(3)126..275(+)
Misc Feature(4)330..515(+)
Misc Feature(5)357..497(+)
Exon (1)1..74
Gene:MYL1
Gene Synonym:
Exon (2)75..99
Gene:MYL1
Gene Synonym:
Exon (3)100..243
Gene:MYL1
Gene Synonym:
Exon (4)244..417
Gene:MYL1
Gene Synonym:
Exon (5)418..495
Gene:MYL1
Gene Synonym:
Exon (6)496..538
Gene:MYL1
Gene Synonym:
Exon (7)539..862
Gene:MYL1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_079422
Organism Homo sapiens (human)
Definition Homo sapiens myosin, light chain 1, alkali; skeletal, fast (MYL1), transcript variant 3f, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_079422

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGTCCTTCA GTGCTGACCA GATTGCTGAA TTCAAGGAGG CATTTCTCCT GTTTGACAGA 
ACAGGTGATT CCAAGATCAC CTTAAGCCAG GTCGGTGATG TCCTTCGAGC TCTGGGCACA
AATCCCACCA ATGCAGAGGT CAGGAAAGTT CTGGGAAACC CCAGCAATGA AGAGCTGAAT
GCCAAGAAAA TTGAGTTTGA ACAATTTCTG CCTATGATGC AAGCCATTTC CAACAACAAG
GACCAGGCCA CCTATGAAGA CTTTGTTGAG GGTCTGCGTG TCTTTGACAA GGAAGGCAAT
GGCACAGTCA TGGGTGCTGA ACTCCGCCAT GTTCTAGCCA CCCTGGGTGA AAAGATGAAA
GAGGAAGAAG TGGAAGCCCT GATGGCAGGT CAAGAAGACT CCAATGGCTG CATCAACTAC
GAAGCTTTTG TCAAGCACAT CATGTCTATC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu13342
Clone ID Related Accession (Same CDS sequence) NM_079420
Accession Version NM_079420.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 28-APR-2015
Organism Homo sapiens (human)
Product myosin light chain 1/3, skeletal muscle isoform isoform 1f
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA898482.1, CR456869.1 and AA705167.1. On Oct 4, 2006 this sequence version replaced gi:17986272. Transcript Variant: This variant (1f) encodes a protein that is 44 aa longer than isoform 3f. Transcript variants 1f and 3f differ in their first two exons. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL598805.1, BF693841.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1970526, SAMEA2142348 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
ATGGCACCAA AGAAAGACGT GAAGAAACCT GTGGCTGCGG CTGCGGCTGC CCCAGCCCCG 
GCACCGGCAC CTGCACCTGC CCCTGCCCCA GCCAAACCCA AAGAAGAAAA AATTGACCTC
TCTGCCATTA AGATCGAGTT CTCTAAGGAA CAGCAGGATG AATTCAAGGA GGCATTTCTC
CTGTTTGACA GAACAGGTGA TTCCAAGATC ACCTTAAGCC AGGTCGGTGA TGTCCTTCGA
GCTCTGGGCA CAAATCCCAC CAATGCAGAG GTCAGGAAAG TTCTGGGAAA CCCCAGCAAT
GAAGAGCTGA ATGCCAAGAA AATTGAGTTT GAACAATTTC TGCCTATGAT GCAAGCCATT
TCCAACAACA AGGACCAGGC CACCTATGAA GACTTTGTTG AGGGTCTGCG TGTCTTTGAC
AAGGAAGGCA ATGGCACAGT CATGGGTGCT GAACTCCGCC ATGTTCTAGC CACCCTGGGT
GAAAAGATGA AAGAGGAAGA AGTGGAAGCC CTGATGGCAG GTCAAGAAGA CTCCAATGGC
TGCATCAACT ACGAAGCTTT TGTCAAGCAC ATCATGTCTA TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_524144.1
CDS130..714
Misc Feature(1)106..108(+)
Misc Feature(2)133..135(+)
Misc Feature(3)262..708(+)
Misc Feature(4)289..483(+)
Misc Feature(5)289..378(+)
Misc Feature(6)316..465(+)
Misc Feature(7)520..705(+)
Misc Feature(8)547..687(+)
Exon (1)1..261
Gene:MYL1
Gene Synonym:
Exon (2)262..289
Gene:MYL1
Gene Synonym:
Exon (3)290..433
Gene:MYL1
Gene Synonym:
Exon (4)434..607
Gene:MYL1
Gene Synonym:
Exon (5)608..685
Gene:MYL1
Gene Synonym:
Exon (6)686..728
Gene:MYL1
Gene Synonym:
Exon (7)729..1052
Gene:MYL1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_079420
Organism Homo sapiens (human)
Definition Homo sapiens myosin, light chain 1, alkali; skeletal, fast (MYL1), transcript variant 1f, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_079420

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCACCAA AGAAAGACGT GAAGAAACCT GTGGCTGCGG CTGCGGCTGC CCCAGCCCCG 
GCACCGGCAC CTGCACCTGC CCCTGCCCCA GCCAAACCCA AAGAAGAAAA AATTGACCTC
TCTGCCATTA AGATCGAGTT CTCTAAGGAA CAGCAGGATG AATTCAAGGA GGCATTTCTC
CTGTTTGACA GAACAGGTGA TTCCAAGATC ACCTTAAGCC AGGTCGGTGA TGTCCTTCGA
GCTCTGGGCA CAAATCCCAC CAATGCAGAG GTCAGGAAAG TTCTGGGAAA CCCCAGCAAT
GAAGAGCTGA ATGCCAAGAA AATTGAGTTT GAACAATTTC TGCCTATGAT GCAAGCCATT
TCCAACAACA AGGACCAGGC CACCTATGAA GACTTTGTTG AGGGTCTGCG TGTCTTTGAC
AAGGAAGGCA ATGGCACAGT CATGGGTGCT GAACTCCGCC ATGTTCTAGC CACCCTGGGT
GAAAAGATGA AAGAGGAAGA AGTGGAAGCC CTGATGGCAG GTCAAGAAGA CTCCAATGGC
TGCATCAACT ACGAAGCTTT TGTCAAGCAC ATCATGTCTA TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

The MLK-related kinase (MRK) is a novel RhoC effector that mediates lysophosphatidic acid (LPA)-stimulated tumor cell invasion
J. Biol. Chem. 288 (8), 5364-5373 (2013)
Korkina O, Dong Z, Marullo A, Warshaw G, Symons M and Ruggieri R.


book

Mutations of ventricular essential myosin light chain disturb myosin binding and sarcomeric sorting
Cardiovasc. Res. 93 (3), 390-396 (2012)
Lossie J, Ushakov DS, Ferenczi MA, Werner S, Keller S, Haase H and Morano I.


book

Ischemia induced peroxynitrite dependent modifications of cardiomyocyte MLC1 increases its degradation by MMP-2 leading to contractile dysfunction
J. Cell. Mol. Med. 15 (5), 1136-1147 (2011)
Polewicz D, Cadete VJ, Doroszko A, Hunter BE, Sawicka J, Szczesna-Cordary D, Light PE and Sawicki G.


book

Inhibition of MLC phosphorylation restricts replication of influenza virus--a mechanism of action for anti-influenza agents
PLoS ONE 6 (6), E21444 (2011)
Haidari M, Zhang W, Ganjehei L, Ali M and Chen Z.


book

Interaction of ADP-ribosylated actin with actin binding proteins
FEBS Lett. 508 (1), 131-135 (2001)
Ballweber E, Galla M, Aktories K, Yeoh S, Weeds AG and Mannherz HG.


book

Characterization of human myosin light chains 1sa and 3nm: implications for isoform evolution and function
Mol. Cell. Biol. 10 (3), 1095-1104 (1990)
Hailstones DL and Gunning PW.


book

Identification of the functional promoter regions in the human gene encoding the myosin alkali light chains MLC1 and MLC3 of fast skeletal muscle
J. Biol. Chem. 264 (27), 16109-16117 (1989)
Seidel U and Arnold HH.


book

Alkali myosin light chains in man are encoded by a multigene family that includes the adult skeletal muscle, the embryonic or atrial, and nonsarcomeric isoforms
Gene 66 (1), 135-146 (1988)
Seidel U, Bober E, Winter B, Lenz S, Lohse P, Goedde HW, Grzeschik KH and Arnold HH.


book

Assignment of the human fast skeletal muscle myosin alkali light chains gene (MLC1F/MLC3F) to 2q 32.1-2qter
Hum. Genet. 78 (1), 65-70 (1988)
Cohen-Haguenauer O, Barton PJ, Nguyen VC, Serero S, Gross MS, Jegou-Foubert C, de Tand MF, Robert B, Buckingham M and Frezal J.


book

The complete nucleotide sequences of cDNA clones coding for human myosin light chains 1 and 3
Nucleic Acids Res. 15 (12), 4989 (1987)
Seidel,U., Bober,E., Winter,B., Lenz,S., Lohse,P. and Arnold,H.H.


 
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