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MYL2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MYL2
Entrez Gene ID 4633
Full Name myosin, light chain 2, regulatory, cardiac, slow
Synonyms CMH10, MLC2
General protein information
Preferred Names
myosin regulatory light chain 2, ventricular/cardiac muscle isoform
Names
myosin regulatory light chain 2, ventricular/cardiac muscle isoform
MLC-2
MLC-2v
RLC of myosin
myosin light chain 2
regulatory light chain of myosin
cardiac ventricular myosin light chain 2
slow cardiac myosin regulatory light chain 2
myosin, light polypeptide 2, regulatory, cardiac, slow
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q24.11

Summary Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

160781

Disorder Html: Cardiomyopathy, familial hypertrophic, 10, 608758 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000432 NP_000423 myosin regulatory light chain 2, ventricular/cardiac muscle isoform



Homo sapiens (human) MYL2 NP_000423.2
Pan troglodytes (chimpanzee) MYL2 XP_001144828.2
Macaca mulatta (Rhesus monkey) MYL2 XP_001100796.1
Canis lupus familiaris (dog) MYL2 NP_001003069.1
Bos taurus (cattle) MYL2 NP_001030197.1
Mus musculus (house mouse) Myl2 NP_034991.3
Rattus norvegicus (Norway rat) Myl2 NP_001030329.2
Gallus gallus (chicken) MYL2 NP_001258858.1
Danio rerio (zebrafish) myl2b NP_001035134.1
Danio rerio (zebrafish) LOC100537244 XP_003198611.2
Xenopus (Silurana) tropicalis (western clawed frog) myl2 NP_001107717.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MYL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18354
NM_000432 Homo sapiens myosin, light chain 2, regulatory, cardiac, slow (MYL2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18354
Clone ID Related Accession (Same CDS sequence) NM_000432
Accession Version NM_000432.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 501bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product myosin regulatory light chain 2, ventricular/cardiac muscle isoform
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AA090235.1, BC015821.1 and BC031008.1. This sequence is a reference standard in the RefSeqGene project. On Jun 14, 2008 this sequence version replaced gi:94981552. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP318509.1, BC031008.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
ATGGCACCTA AGAAAGCAAA GAAGAGAGCC GGGGGCGCCA ACTCCAACGT GTTCTCCATG 
TTCGAACAGA CCCAAATCCA GGAATTTAAG GAGGCCTTCA CTATCATGGA CCAGAACAGG
GATGGCTTCA TTGACAAGAA CGATCTGAGA GACACCTTTG CTGCCCTTGG GCGAGTGAAC
GTGAAAAATG AAGAAATTGA TGAAATGATC AAGGAGGCTC CGGGTCCAAT TAACTTTACT
GTGTTCCTCA CAATGTTTGG GGAGAAACTT AAGGGAGCGG ACCCTGAGGA AACCATTCTC
AACGCATTCA AAGTGTTTGA CCCTGAAGGC AAAGGGGTGC TGAAGGCTGA TTACGTTCGG
GAAATGCTGA CCACGCAGGC GGAGAGGTTT TCCAAGGAGG AGGTTGACCA GATGTTCGCC
GCCTTCCCCC CTGACGTGAC TGGCAACTTG GACTACAAGA ACCTGGTGCA CATCATCACC
CACGGAGAAG AGAAGGACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000423.2
CDS72..572
Misc Feature(1)114..560(+)
Misc Feature(2)126..128(+)
Misc Feature(3)153..>290(+)
Misc Feature(4)180..215(+)
Misc Feature(5)375..551(+)
Misc Feature(6)390..533(+)
Exon (1)1..74
Gene:MYL2
Gene Synonym:
Exon (2)75..164
Gene:MYL2
Gene Synonym:
Exon (3)165..240
Gene:MYL2
Gene Synonym:
Exon (4)241..345
Gene:MYL2
Gene Synonym:
Exon (5)346..424
Gene:MYL2
Gene Synonym:
Exon (6)425..473
Gene:MYL2
Gene Synonym:
Exon (7)474..830
Gene:MYL2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000432
Organism Homo sapiens (human)
Definition Homo sapiens myosin, light chain 2, regulatory, cardiac, slow (MYL2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000432

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGCACCTA AGAAAGCAAA GAAGAGAGCC GGGGGCGCCA ACTCCAACGT GTTCTCCATG 
TTCGAACAGA CCCAAATCCA GGAATTTAAG GAGGCCTTCA CTATCATGGA CCAGAACAGG
GATGGCTTCA TTGACAAGAA CGATCTGAGA GACACCTTTG CTGCCCTTGG GCGAGTGAAC
GTGAAAAATG AAGAAATTGA TGAAATGATC AAGGAGGCTC CGGGTCCAAT TAACTTTACT
GTGTTCCTCA CAATGTTTGG GGAGAAACTT AAGGGAGCGG ACCCTGAGGA AACCATTCTC
AACGCATTCA AAGTGTTTGA CCCTGAAGGC AAAGGGGTGC TGAAGGCTGA TTACGTTCGG
GAAATGCTGA CCACGCAGGC GGAGAGGTTT TCCAAGGAGG AGGTTGACCA GATGTTCGCC
GCCTTCCCCC CTGACGTGAC TGGCAACTTG GACTACAAGA ACCTGGTGCA CATCATCACC
CACGGAGAAG AGAAGGACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Potential regulation of human muscle plasticity by MLC2 post-translational modifications during bed rest and countermeasures
Arch. Biochem. Biophys. 540 (1-2), 125-132 (2013)
Stevens L, Bastide B, Hedou J, Cieniewski-Bernard C, Montel V, Cochon L, Dupont E and Mounier Y.


book

New susceptibility loci in MYL2, C12orf51 and OAS1 associated with 1-h plasma glucose as predisposing risk factors for type 2 diabetes in the Korean population
J. Hum. Genet. 58 (6), 362-365 (2013)
Go MJ, Hwang JY, Kim YJ, Hee Oh J, Kim YJ, Heon Kwak S, Soo Park K, Lee J, Kim BJ, Han BG, Cho MC, Cho YS and Lee JY.


book

A genome-wide association study of a coronary artery disease risk variant
J. Hum. Genet. 58 (3), 120-126 (2013)
Lee JY, Lee BS, Shin DJ, Woo Park K, Shin YA, Joong Kim K, Heo L, Young Lee J, Kyoung Kim Y, Jin Kim Y, Bum Hong C, Lee SH, Yoon D, Jung Ku H, Oh IY, Kim BJ, Lee J, Park SJ, Kim J, Kawk HK, Lee JE, Park HK, Lee JE, Nam HY, Park HY, Shin C, Yokota M, Asano H, Nakatochi M, Matsubara T, Kitajima H, Yamamoto K, Kim HL, Han BG, Cho MC, Jang Y, Kim HS, Euy Park J and Lee JY.


book

Common variants at 12q24 are associated with drinking behavior in Han Chinese
Am. J. Clin. Nutr. 97 (3), 545-551 (2013)
Yang X, Lu X, Wang L, Chen S, Li J, Cao J, Chen J, Hao Y, Li Y, Zhao L, Li H, Liu D, Wang L, Lu F, Shen C, Yu L, Wu X, Zhao Q, Ji X, Guo D, Peng X, Huang J and Gu D.


book

Recessive MYL2 mutations cause infantile type I muscle fibre disease and cardiomyopathy
Brain 136 (PT 1), 282-293 (2013)
Weterman MA, Barth PG, van Spaendonck-Zwarts KY, Aronica E, Poll-The BT, Brouwer OF, van Tintelen JP, Qahar Z, Bradley EJ, de Wissel M, Salviati L, Angelini C, van den Heuvel L, Thomasse YE, Backx AP, Nurnberg G, Nurnberg P and Baas F.


book

The major protein expression profile and two-dimensional protein database of human heart
Electrophoresis 16 (7), 1160-1169 (1995)
Kovalyov LI, Shishkin SS, Efimochkin AS, Kovalyova MA, Ershova ES, Egorov TA and Musalyamov AK.


book

Hypertrophic Cardiomyopathy Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Cirino,A.L. and Ho,C.


book

Interaction of a conserved peptide domain in recombinant human ventricular myosin light chain-2 with myosin heavy chain
Cell. Mol. Biol. Res. 39 (1), 13-26 (1993)
Wadgaonkar R, Shafiq S, Rajmanickam C and Siddiqui MA.


book

Localization of the gene coding for ventricular myosin regulatory light chain (MYL2) to human chromosome 12q23-q24.3
Genomics 13 (3), 829-831 (1992)
Macera MJ, Szabo P, Wadgaonkar R, Siddiqui MA and Verma RS.


book

Isolation and nucleotide sequence of the cDNA encoding human ventricular myosin light chain 2
Nucleic Acids Res. 17 (6), 2360 (1989)
Dalla Libera L, Hoffmann E, Floroff M and Jackowski G.


 
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