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MYL3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MYL3
Entrez Gene ID 4634
Full Name myosin, light chain 3, alkali; ventricular, skeletal, slow
Synonyms CMH8, MLC1SB, MLC1V, VLC1
General protein information
Preferred Names
myosin light chain 3
Names
myosin light chain 3
CMLC1
cardiac myosin light chain 1
ventricular/slow twitch myosin alkali light chain
myosin light chain 1, slow-twitch muscle B/ventricular isoform
myosin, light polypeptide 3, alkali; ventricular, skeletal, slow
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

3

3p21.3-p21.2

Summary MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

160790

Disorder Html: Cardiomyopathy, familial hypertrophic, 8, 608751 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000258 NP_000249 myosin light chain 3



Homo sapiens (human) MYL3 NP_000249.1
Pan troglodytes (chimpanzee) LOC460327 XP_003309811.2
Macaca mulatta (Rhesus monkey) MYL3 XP_001113910.1
Canis lupus familiaris (dog) MYL3 XP_005632679.1
Bos taurus (cattle) MYL3 NP_001069969.2
Mus musculus (house mouse) Myl3 NP_034989.1
Rattus norvegicus (Norway rat) Myl3 NP_036738.1
Gallus gallus (chicken) MYL3 NP_990490.1
Danio rerio (zebrafish) zgc:66286 NP_956810.1
Xenopus (Silurana) tropicalis (western clawed frog) myl3 NP_989012.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MYL3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYL3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18259
NM_000258 Homo sapiens myosin, light chain 3, alkali; ventricular, skeletal, slow (MYL3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18259
Clone ID Related Accession (Same CDS sequence) NM_000258
Accession Version NM_000258.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 588bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product myosin light chain 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP316922.1 and AJ709328.1. This sequence is a reference standard in the RefSeqGene project. On Oct 4, 2006 this sequence version replaced gi:4557776. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009790.1, CF552959.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154361, SAMEA962337 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGCCCCCA AAAAGCCAGA GCCCAAGAAG GATGATGCCA AGGCAGCCCC CAAGGCAGCT 
CCAGCTCCCG CACCTCCCCC TGAGCCTGAG CGCCCTAAGG AGGTCGAGTT TGATGCTTCC
AAGATCAAGA TTGAGTTCAC ACCTGAGCAG ATTGAAGAGT TCAAGGAAGC CTTCATGCTG
TTCGACCGCA CACCCAAGTG TGAGATGAAG ATCACCTACG GGCAGTGTGG GGATGTCCTG
CGGGCGCTGG GCCAGAACCC CACACAGGCA GAAGTGCTCC GTGTCCTGGG GAAGCCAAGA
CAGGAAGAGC TCAATACCAA GATGATGGAC TTTGAAACTT TCCTGCCTAT GCTCCAGCAC
ATTTCCAAGA ACAAGGACAC AGGCACCTAT GAGGACTTCG TGGAGGGGCT GCGGGTCTTC
GACAAGGAGG GCAATGGCAC TGTCATGGGT GCTGAGCTTC GCCACGTGCT GGCCACGCTG
GGTGAGAGGC TGACAGAAGA CGAAGTGGAG AAGTTGATGG CTGGGCAAGA GGACTCCAAT
GGCTGCATCA ACTATGAAGC ATTTGTGAAG CACATCATGT CCAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000249.1
CDS94..681
Misc Feature(1)25..27(+)
Misc Feature(2)223..675(+)
Misc Feature(3)487..672(+)
Misc Feature(4)496..498(+)
Misc Feature(5)514..654(+)
Exon (1)1..222
Gene:MYL3
Gene Synonym:
Exon (2)223..250
Gene:MYL3
Gene Synonym:
Exon (3)251..400
Gene:MYL3
Gene Synonym:
Exon (4)401..574
Gene:MYL3
Gene Synonym:
Exon (5)575..652
Gene:MYL3
Gene Synonym:
Exon (6)653..694
Gene:MYL3
Gene Synonym:
Exon (7)695..929
Gene:MYL3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000258
Organism Homo sapiens (human)
Definition Homo sapiens myosin, light chain 3, alkali; ventricular, skeletal, slow (MYL3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000258

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCCCCCA AAAAGCCAGA GCCCAAGAAG GATGATGCCA AGGCAGCCCC CAAGGCAGCT 
CCAGCTCCCG CACCTCCCCC TGAGCCTGAG CGCCCTAAGG AGGTCGAGTT TGATGCTTCC
AAGATCAAGA TTGAGTTCAC ACCTGAGCAG ATTGAAGAGT TCAAGGAAGC CTTCATGCTG
TTCGACCGCA CACCCAAGTG TGAGATGAAG ATCACCTACG GGCAGTGTGG GGATGTCCTG
CGGGCGCTGG GCCAGAACCC CACACAGGCA GAAGTGCTCC GTGTCCTGGG GAAGCCAAGA
CAGGAAGAGC TCAATACCAA GATGATGGAC TTTGAAACTT TCCTGCCTAT GCTCCAGCAC
ATTTCCAAGA ACAAGGACAC AGGCACCTAT GAGGACTTCG TGGAGGGGCT GCGGGTCTTC
GACAAGGAGG GCAATGGCAC TGTCATGGGT GCTGAGCTTC GCCACGTGCT GGCCACGCTG
GGTGAGAGGC TGACAGAAGA CGAAGTGGAG AAGTTGATGG CTGGGCAAGA GGACTCCAAT
GGCTGCATCA ACTATGAAGC ATTTGTGAAG CACATCATGT CCAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Furthering the link between the sarcomere and primary cardiomyopathies: restrictive cardiomyopathy associated with multiple mutations in genes previously associated with hypertrophic or dilated cardiomyopathy
Am. J. Med. Genet. A 155A (9), 2229-2235 (2011)
Caleshu C, Sakhuja R, Nussbaum RL, Schiller NB, Ursell PC, Eng C, De Marco T, McGlothlin D, Burchard EG and Rame JE.


book

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study
Diabetes Care 33 (10), 2250-2253 (2010)
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC and Anand S.


book

Clinical features and outcome of hypertrophic cardiomyopathy associated with triple sarcomere protein gene mutations
J. Am. Coll. Cardiol. 55 (14), 1444-1453 (2010)
Girolami F, Ho CY, Semsarian C, Baldi M, Will ML, Baldini K, Torricelli F, Yeates L, Cecchi F, Ackerman MJ and Olivotto I.


book

Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip
Am. J. Hum. Genet. 85 (5), 628-642 (2009)
Talmud PJ, Drenos F, Shah S, Shah T, Palmen J, Verzilli C, Gaunt TR, Pallas J, Lovering R, Li K, Casas JP, Sofat R, Kumari M, Rodriguez S, Johnson T, Newhouse SJ, Dominiczak A, Samani NJ, Caulfield M, Sever P, Stanton A, Shields DC, Padmanabhan S, Melander O, Hastie C, Delles C, Ebrahim S, Marmot MG, Smith GD, Lawlor DA, Munroe PB, Day IN, Kivimaki M, Whittaker J, Humphries SE and Hingorani AD.


book

Prevalence of sarcomere protein gene mutations in preadolescent children with hypertrophic cardiomyopathy
Circ Cardiovasc Genet 2 (5), 436-441 (2009)
Kaski JP, Syrris P, Esteban MT, Jenkins S, Pantazis A, Deanfield JE, McKenna WJ and Elliott PM.


book

Hypertrophic Cardiomyopathy Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Cirino,A.L. and Ho,C.


book

Analysis of the upstream regulatory region of human ventricular myosin light chain 1 gene
J. Mol. Cell. Cardiol. 24 (11), 1221-1229 (1992)
Shi Q, Li RK, Mickle DA and Jackowski G.


book

Human ventricular/slow twitch myosin alkali light chain gene characterization, sequence, and chromosomal location
J. Biol. Chem. 264 (4), 2143-2149 (1989)
Fodor WL, Darras B, Seharaseyon J, Falkenthal S, Francke U and Vanin EF.


book

Chromosomal assignment of two myosin alkali light-chain genes encoding the ventricular/slow skeletal muscle isoform and the atrial/fetal muscle isoform (MYL3, MYL4)
Hum. Genet. 81 (3), 278-282 (1989)
Cohen-Haguenauer O, Barton PJ, Van Cong N, Cohen A, Masset M, Buckingham M and Frezal J.


book

Chromosomal rearrangement generating a composite gene for a developmental transcription factor
Science 243 (4890), 507-512 (1989)
Stragier P, Kunkel B, Kroos L and Losick R.


 
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