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MYL4 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MYL4
Entrez Gene ID 4635
Full Name myosin, light chain 4, alkali; atrial, embryonic
Synonyms ALC1, AMLC, GT1, PRO1957
General protein information
Preferred Names
myosin light chain 4
Names
myosin light chain 4
myosin light chain alkali GT-1 isoform
myosin, atrial/fetal muscle, light chain
myosin light chain 1, embryonic muscle/atrial isoform
myosin, light polypeptide 4, alkali; atrial, embryonic
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q21.32

Summary Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

160770


mRNA and Protein(s)

mRNA Protein Name
XM_005257391 XP_005257448 myosin light chain 4 isoform X1
XM_011524838 XP_011523140 myosin light chain 4 isoform X1
XM_011524839 XP_011523141 myosin light chain 4 isoform X2
NM_002476 NP_002467 myosin light chain 4
NM_001002841 NP_001002841 myosin light chain 4



Homo sapiens (human) MYL4 NP_002467.1
Pan troglodytes (chimpanzee) MYL4 XP_511623.1
Macaca mulatta (Rhesus monkey) LOC717354 XP_001116001.1
Canis lupus familiaris (dog) MYL4 XP_005624249.1
Bos taurus (cattle) MYL4 NP_001068617.1
Mus musculus (house mouse) Myl4 NP_034988.2
Rattus norvegicus (Norway rat) Myl4 NP_001102965.1
Gallus gallus (chicken) MYL4 XP_003642828.1
Danio rerio (zebrafish) myl4 NP_001020354.1
Xenopus (Silurana) tropicalis (western clawed frog) myl4 NP_001096232.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MYL4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYL4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu30031
XM_005257391 PREDICTED: Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu30031
XM_011524838 PREDICTED: Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu59144 XM_011524839 PREDICTED: Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
OHu30031
NM_002476 Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu30031
NM_001002841 Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu30031
Clone ID Related Accession (Same CDS sequence) NM_001002841 , NM_002476 , XM_005257391 , XM_011524838
Accession Version XM_005257391.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 594bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product myosin light chain 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578830985. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
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481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005257448.1
CDS114..707
Misc Feature(1)258..701(+)
Misc Feature(2)513..698(+)
Misc Feature(3)540..680(+)
Translation

Target ORF information:

RefSeq Version XM_005257391
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257391

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu30031
Clone ID Related Accession (Same CDS sequence) NM_001002841 , NM_002476 , XM_005257391 , XM_011524838
Accession Version XM_011524838.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 594bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product myosin light chain 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523140.1
CDS131..724
Misc Feature(1)275..718(+)
Misc Feature(2)530..715(+)
Misc Feature(3)557..697(+)
Translation

Target ORF information:

RefSeq Version XM_011524838
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524838

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59144
Clone ID Related Accession (Same CDS sequence) XM_011524839
Accession Version XM_011524839.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 384bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product myosin light chain 4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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61
121
181
241
301
361
ATGAAGATCA CCTACGGCCA GTGCGGGGAT GTACTGCGGG CCCTGGGCCA GAACCCTACC 
AATGCCGAGG TGCTGCGTGT GCTGGGCAAG CCCAAGCCTG AAGAGATGAA TGTCAAGATG
CTGGACTTTG AGACGTTCTT GCCCATCCTG CAGCACATTT CCCGCAACAA GGAGCAGGGC
ACCTATGAGG ACTTCGTGGA GGGCCTGCGT GTCTTTGACA AGGAGAGCAA TGGCACGGTC
ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC ACCCTGGGAG AGAAGATGAC TGAGGCTGAA
GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT GCCAATGGCT GCATCAATTA TGAAGCCTTT
GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523141.1
CDS135..518
Misc Feature(1)324..509(+)
Misc Feature(2)342..500(+)
Misc Feature(3)351..491(+)
Translation

Target ORF information:

RefSeq Version XM_011524839
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524839

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGAAGATCA CCTACGGCCA GTGCGGGGAT GTACTGCGGG CCCTGGGCCA GAACCCTACC 
AATGCCGAGG TGCTGCGTGT GCTGGGCAAG CCCAAGCCTG AAGAGATGAA TGTCAAGATG
CTGGACTTTG AGACGTTCTT GCCCATCCTG CAGCACATTT CCCGCAACAA GGAGCAGGGC
ACCTATGAGG ACTTCGTGGA GGGCCTGCGT GTCTTTGACA AGGAGAGCAA TGGCACGGTC
ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC ACCCTGGGAG AGAAGATGAC TGAGGCTGAA
GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT GCCAATGGCT GCATCAATTA TGAAGCCTTT
GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu30031
Clone ID Related Accession (Same CDS sequence) NM_001002841 , NM_002476 , XM_005257391 , XM_011524838
Accession Version NM_002476.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 594bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product myosin light chain 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X58851.1. On Dec 18, 2001 this sequence version replaced gi:4557037. Transcript Variant: This variant (2) lacks the 5' exon but has an alternate 5' end, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC030228.1, BM919855.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002467.1
CDS76..669
Misc Feature(1)220..663(+)
Misc Feature(2)271..273(+)
Misc Feature(3)475..660(+)
Misc Feature(4)502..642(+)
Misc Feature(5)661..663(+)
Exon (1)1..210
Gene:MYL4
Gene Synonym:
Exon (2)211..238
Gene:MYL4
Gene Synonym:
Exon (3)239..388
Gene:MYL4
Gene Synonym:
Exon (4)389..562
Gene:MYL4
Gene Synonym:
Exon (5)563..640
Gene:MYL4
Gene Synonym:
Exon (6)641..684
Gene:MYL4
Gene Synonym:
Exon (7)685..855
Gene:MYL4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002476
Organism Homo sapiens (human)
Definition Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002476

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu30031
Clone ID Related Accession (Same CDS sequence) NM_001002841 , NM_002476 , XM_005257391 , XM_011524838
Accession Version NM_001002841.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 594bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product myosin light chain 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from H83803.1, BU658678.1 and BC030228.1. Transcript Variant: This variant (1) represents the longer transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC030228.1, AF116676.2 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001002841.1
CDS129..722
Misc Feature(1)90..92(+)
Misc Feature(2)273..716(+)
Misc Feature(3)324..326(+)
Misc Feature(4)528..713(+)
Misc Feature(5)555..695(+)
Misc Feature(6)714..716(+)
Exon (1)1..94
Gene:MYL4
Gene Synonym:
Exon (2)95..263
Gene:MYL4
Gene Synonym:
Exon (3)264..291
Gene:MYL4
Gene Synonym:
Exon (4)292..441
Gene:MYL4
Gene Synonym:
Exon (5)442..615
Gene:MYL4
Gene Synonym:
Exon (6)616..693
Gene:MYL4
Gene Synonym:
Exon (7)694..737
Gene:MYL4
Gene Synonym:
Exon (8)738..908
Gene:MYL4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001002841
Organism Homo sapiens (human)
Definition Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001002841

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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