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MYL4 myosin, light chain 4, alkali; atrial, embryonic [Homo sapiens (human)]

Gene Symbol MYL4
Entrez Gene ID 4635
Full Name myosin, light chain 4, alkali; atrial, embryonic
Synonyms ALC1, AMLC, GT1, PRO1957
General protein information
Preferred Names
myosin light chain 4
Names
myosin light chain 4
myosin light chain alkali GT-1 isoform
myosin, atrial/fetal muscle, light chain
myosin light chain 1, embryonic muscle/atrial isoform
myosin, light polypeptide 4, alkali; atrial, embryonic
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q21.32

Summary Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

160770

Disorder Html:





The following MYL4 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYL4 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu30031 XM_005257391 PREDICTED: Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.3
$99
OHu30031 XM_011524838 PREDICTED: Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.3
$99
OHu59144 XM_011524839 PREDICTED: Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $99
OHu30031 NM_002476 Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.3
$99
OHu30031 NM_001002841 Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.3
$99

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu30031
Accession Version XM_005257391.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 594bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product myosin light chain 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578830985. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005257448.1
CDS114..707
Misc Feature(1)258..701(+)
Misc Feature(2)513..698(+)
Misc Feature(3)540..680(+)
Translation
Position Chain Variation Link
17 17 c, t dbSNP:372608767
41 41 c, t dbSNP:373225388
61 61 c, t dbSNP:568252930
83 83 a, g dbSNP:757348664
84 84 c, g, t dbSNP:781256182
86 86 c, t dbSNP:373169208
94 94 a, g dbSNP:775229902
105 105 c, t dbSNP:202127040
106 106 -, a dbSNP:773876585
108 108 a, g dbSNP:749072621
111 111 a, c dbSNP:370578383
128 128 g, t dbSNP:773929956
143 143 a, g dbSNP:140092425
151 151 a, c, t dbSNP:767067527
155 155 -, ccagct dbSNP:774642552
156 156 -, ccagctccagct dbSNP:763415247
156 156 -, ccagct dbSNP:766730973
160 160 c, g dbSNP:760072257
170 170 a, g dbSNP:765876288
177 177 g, t dbSNP:14793
178 178 c, t dbSNP:753082892
179 179 c, t dbSNP:201301071
181 181 c, g dbSNP:764371297
185 185 a, c, g dbSNP:370547497
199 199 c, t dbSNP:143784817
202 202 c, t dbSNP:781196896
212 212 -, ccc dbSNP:759787679
216 216 a, g dbSNP:750529340
222 222 a, c dbSNP:756010629
226 226 a, c, g, t dbSNP:780020430
228 228 -, t dbSNP:767743711
229 229 g, t dbSNP:778776213
232 232 a, t dbSNP:747700234
238 238 a, g dbSNP:771875522
241 241 a, g dbSNP:146860407
249 249 a, g dbSNP:140603047
263 263 c, t dbSNP:143234546
264 264 a, c, g dbSNP:772358836
266 266 c, g dbSNP:760839785
278 278 g, t dbSNP:1135060
279 279 g, t dbSNP:775677626
280 280 c, t dbSNP:144870368
283 283 a, c dbSNP:768696948
289 289 c, t dbSNP:199785200
292 292 c, t dbSNP:761664359
306 306 c, t dbSNP:767289051
313 313 c, t dbSNP:201579845
314 314 a, g dbSNP:149036573
318 318 a, g dbSNP:766060796
320 320 a, t dbSNP:753325178
321 321 a, g dbSNP:754540258
325 325 g, t dbSNP:527647806
332 332 c, g, t dbSNP:752046263
338 338 c, t dbSNP:372355856
339 339 a, g, t dbSNP:375596244
344 344 c, g, t dbSNP:143062804
346 346 a, g dbSNP:768793001
347 347 -, c dbSNP:776650813
347 347 c, t dbSNP:138710476
348 348 a, g dbSNP:748072025
350 350 a, g dbSNP:771938007
360 360 c, t dbSNP:773030593
361 361 a, g dbSNP:760514647
384 384 -, at dbSNP:768546854
385 385 a, g dbSNP:142769650
390 390 a, g dbSNP:369894969
398 398 a, g dbSNP:776333344
400 400 a, g dbSNP:146980908
420 420 c, t dbSNP:764678212
428 428 a, g dbSNP:762424935
429 429 a, g dbSNP:768062115
430 430 c, t dbSNP:750824303
431 431 a, g dbSNP:138156448
437 437 c, t dbSNP:565843103
452 452 g, t dbSNP:200195383
457 457 c, t dbSNP:199794933
458 458 a, g dbSNP:372522881
461 461 c, t dbSNP:184779796
464 464 a, g dbSNP:756774414
465 465 c, t dbSNP:202080282
466 466 c, g dbSNP:537576399
474 474 c, t dbSNP:777913899
478 478 a, g dbSNP:376695433
481 481 c, t dbSNP:141766103
483 483 c, t dbSNP:200415936
486 486 c, t dbSNP:745710105
487 487 a, g dbSNP:111293783
489 489 a, g dbSNP:758768590
494 494 a, g dbSNP:557505985
508 508 a, t dbSNP:201117675
509 509 c, t dbSNP:746843611
518 518 c, t dbSNP:150593274
519 519 a, g dbSNP:375241929
527 527 c, t dbSNP:13589
531 531 c, t dbSNP:767035280
532 532 a, g dbSNP:374127769
541 541 a, g dbSNP:201076527
542 542 c, t dbSNP:139239258
548 548 a, g dbSNP:765494388
550 550 a, g dbSNP:752796010
559 559 c, t dbSNP:200236286
560 560 a, g dbSNP:371944933
566 566 -, g dbSNP:771396667
579 579 c, t dbSNP:553621405
580 580 a, g dbSNP:757162928
584 584 c, t dbSNP:781162398
585 585 a, g dbSNP:745655031
604 604 -, aga dbSNP:774717295
612 612 a, g dbSNP:535742062
624 624 a, g dbSNP:763432981
625 625 c, t dbSNP:764294751
631 631 a, c, g dbSNP:373421058
635 635 a, g dbSNP:767533462
650 650 a, g dbSNP:181540609
657 657 a, g dbSNP:755977099
661 661 g, t dbSNP:4784
662 662 c, t dbSNP:766393545
669 669 a, t dbSNP:16941677
670 670 a, g dbSNP:754888534
672 672 a, t dbSNP:549012048
679 679 a, c dbSNP:758099887
680 680 c, t dbSNP:777538559
697 697 c, t dbSNP:751027502
703 703 a, g dbSNP:756719485
712 712 a, g dbSNP:753462043
715 715 -, t dbSNP:767688792
721 721 a, g dbSNP:759136865
737 737 g, t dbSNP:10930
753 753 c, g dbSNP:770337731
765 765 a, g dbSNP:572701811
807 807 g, t dbSNP:752148801
844 844 a, c dbSNP:149130670
880 880 c, t dbSNP:561960570

Target ORF information:

RefSeq Version XM_005257391
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257391

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu30031
Accession Version XM_011524838.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 594bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product myosin light chain 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011523140.1
CDS131..724
Misc Feature(1)275..718(+)
Misc Feature(2)530..715(+)
Misc Feature(3)557..697(+)
Translation
Position Chain Variation Link
6 6 a, g dbSNP:556863863
12 12 a, g dbSNP:182025742
31 31 g, t dbSNP:186277396
37 37 a, g dbSNP:780663194
50 50 c, g dbSNP:553459170
71 71 c, t dbSNP:573384799
74 74 c, t dbSNP:574540052
76 76 c, t dbSNP:150616965
77 77 a, g dbSNP:111876061
81 81 c, g dbSNP:199619967
83 83 c, t dbSNP:758606614
84 84 a, g dbSNP:369748183
94 94 c, t dbSNP:1134983
100 100 a, g dbSNP:757348664
101 101 c, g, t dbSNP:781256182
103 103 c, t dbSNP:373169208
111 111 a, g dbSNP:775229902
122 122 c, t dbSNP:202127040
123 123 -, a dbSNP:773876585
125 125 a, g dbSNP:749072621
128 128 a, c dbSNP:370578383
145 145 g, t dbSNP:773929956
160 160 a, g dbSNP:140092425
168 168 a, c, t dbSNP:767067527
172 172 -, ccagct dbSNP:774642552
173 173 -, ccagctccagct dbSNP:763415247
173 173 -, ccagct dbSNP:766730973
177 177 c, g dbSNP:760072257
187 187 a, g dbSNP:765876288
194 194 g, t dbSNP:14793
195 195 c, t dbSNP:753082892
196 196 c, t dbSNP:201301071
198 198 c, g dbSNP:764371297
202 202 a, c, g dbSNP:370547497
216 216 c, t dbSNP:143784817
219 219 c, t dbSNP:781196896
229 229 -, ccc dbSNP:759787679
233 233 a, g dbSNP:750529340
239 239 a, c dbSNP:756010629
243 243 a, c, g, t dbSNP:780020430
245 245 -, t dbSNP:767743711
246 246 g, t dbSNP:778776213
249 249 a, t dbSNP:747700234
255 255 a, g dbSNP:771875522
258 258 a, g dbSNP:146860407
266 266 a, g dbSNP:140603047
280 280 c, t dbSNP:143234546
281 281 a, c, g dbSNP:772358836
283 283 c, g dbSNP:760839785
295 295 g, t dbSNP:1135060
296 296 g, t dbSNP:775677626
297 297 c, t dbSNP:144870368
300 300 a, c dbSNP:768696948
306 306 c, t dbSNP:199785200
309 309 c, t dbSNP:761664359
323 323 c, t dbSNP:767289051
330 330 c, t dbSNP:201579845
331 331 a, g dbSNP:149036573
335 335 a, g dbSNP:766060796
337 337 a, t dbSNP:753325178
338 338 a, g dbSNP:754540258
342 342 g, t dbSNP:527647806
349 349 c, g, t dbSNP:752046263
355 355 c, t dbSNP:372355856
356 356 a, g, t dbSNP:375596244
361 361 c, g, t dbSNP:143062804
363 363 a, g dbSNP:768793001
364 364 -, c dbSNP:776650813
364 364 c, t dbSNP:138710476
365 365 a, g dbSNP:748072025
367 367 a, g dbSNP:771938007
377 377 c, t dbSNP:773030593
378 378 a, g dbSNP:760514647
401 401 -, at dbSNP:768546854
402 402 a, g dbSNP:142769650
407 407 a, g dbSNP:369894969
415 415 a, g dbSNP:776333344
417 417 a, g dbSNP:146980908
437 437 c, t dbSNP:764678212
445 445 a, g dbSNP:762424935
446 446 a, g dbSNP:768062115
447 447 c, t dbSNP:750824303
448 448 a, g dbSNP:138156448
454 454 c, t dbSNP:565843103
469 469 g, t dbSNP:200195383
474 474 c, t dbSNP:199794933
475 475 a, g dbSNP:372522881
478 478 c, t dbSNP:184779796
481 481 a, g dbSNP:756774414
482 482 c, t dbSNP:202080282
483 483 c, g dbSNP:537576399
491 491 c, t dbSNP:777913899
495 495 a, g dbSNP:376695433
498 498 c, t dbSNP:141766103
500 500 c, t dbSNP:200415936
503 503 c, t dbSNP:745710105
504 504 a, g dbSNP:111293783
506 506 a, g dbSNP:758768590
511 511 a, g dbSNP:557505985
525 525 a, t dbSNP:201117675
526 526 c, t dbSNP:746843611
535 535 c, t dbSNP:150593274
536 536 a, g dbSNP:375241929
544 544 c, t dbSNP:13589
548 548 c, t dbSNP:767035280
549 549 a, g dbSNP:374127769
558 558 a, g dbSNP:201076527
559 559 c, t dbSNP:139239258
565 565 a, g dbSNP:765494388
567 567 a, g dbSNP:752796010
576 576 c, t dbSNP:200236286
577 577 a, g dbSNP:371944933
583 583 -, g dbSNP:771396667
596 596 c, t dbSNP:553621405
597 597 a, g dbSNP:757162928
601 601 c, t dbSNP:781162398
602 602 a, g dbSNP:745655031
621 621 -, aga dbSNP:774717295
629 629 a, g dbSNP:535742062
641 641 a, g dbSNP:763432981
642 642 c, t dbSNP:764294751
648 648 a, c, g dbSNP:373421058
652 652 a, g dbSNP:767533462
667 667 a, g dbSNP:181540609
674 674 a, g dbSNP:755977099
678 678 g, t dbSNP:4784
679 679 c, t dbSNP:766393545
686 686 a, t dbSNP:16941677
687 687 a, g dbSNP:754888534
689 689 a, t dbSNP:549012048
696 696 a, c dbSNP:758099887
697 697 c, t dbSNP:777538559
714 714 c, t dbSNP:751027502
720 720 a, g dbSNP:756719485
729 729 a, g dbSNP:753462043
732 732 -, t dbSNP:767688792
738 738 a, g dbSNP:759136865
743 743 c, t dbSNP:538822672
751 751 -, caa dbSNP:745905797
788 788 c, t dbSNP:141227320

Target ORF information:

RefSeq Version XM_011524838
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524838

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59144
Accession Version XM_011524839.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 384bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product myosin light chain 4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
ATGAAGATCA CCTACGGCCA GTGCGGGGAT GTACTGCGGG CCCTGGGCCA GAACCCTACC 
AATGCCGAGG TGCTGCGTGT GCTGGGCAAG CCCAAGCCTG AAGAGATGAA TGTCAAGATG
CTGGACTTTG AGACGTTCTT GCCCATCCTG CAGCACATTT CCCGCAACAA GGAGCAGGGC
ACCTATGAGG ACTTCGTGGA GGGCCTGCGT GTCTTTGACA AGGAGAGCAA TGGCACGGTC
ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC ACCCTGGGAG AGAAGATGAC TGAGGCTGAA
GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT GCCAATGGCT GCATCAATTA TGAAGCCTTT
GTCAAGCACA TCATGTCAGG GTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011523141.1
CDS135..518
Misc Feature(1)324..509(+)
Misc Feature(2)342..500(+)
Misc Feature(3)351..491(+)
Translation
Position Chain Variation Link
2 2 c, t dbSNP:766655631
11 11 c, g dbSNP:368658322
20 20 c, t dbSNP:370946052
21 21 a, g dbSNP:373073361
22 22 c, t dbSNP:200255259
29 29 c, t dbSNP:752613718
32 32 a, g dbSNP:758421366
36 36 c, t dbSNP:186267701
39 39 -, gga, ggagga dbSNP:138017390
40 40 -, ggagga dbSNP:757182904
40 40 -, gga dbSNP:760178523
42 42 -, gga dbSNP:748243221
43 43 c, g dbSNP:751428281
47 47 a, g dbSNP:756825397
50 50 g, t dbSNP:780942692
55 55 a, g dbSNP:745353210
61 61 a, g dbSNP:114308079
64 64 -, gag dbSNP:758056647
65 65 a, g dbSNP:779547933
66 66 a, g dbSNP:748599728
71 71 -, agt dbSNP:779882047
71 71 a, g dbSNP:772592691
76 76 -, gtt dbSNP:746468880
77 77 c, t dbSNP:773528239
79 79 a, g, t dbSNP:747442724
85 85 a, g dbSNP:776969664
89 89 g, t dbSNP:1135060
90 90 g, t dbSNP:775677626
91 91 c, t dbSNP:144870368
94 94 a, c dbSNP:768696948
100 100 c, t dbSNP:199785200
103 103 c, t dbSNP:761664359
117 117 c, t dbSNP:767289051
124 124 c, t dbSNP:201579845
125 125 a, g dbSNP:149036573
129 129 a, g dbSNP:766060796
131 131 a, t dbSNP:753325178
132 132 a, g dbSNP:754540258
136 136 g, t dbSNP:527647806
143 143 c, g, t dbSNP:752046263
149 149 c, t dbSNP:372355856
150 150 a, g, t dbSNP:375596244
155 155 c, g, t dbSNP:143062804
157 157 a, g dbSNP:768793001
158 158 -, c dbSNP:776650813
158 158 c, t dbSNP:138710476
159 159 a, g dbSNP:748072025
161 161 a, g dbSNP:771938007
171 171 c, t dbSNP:773030593
172 172 a, g dbSNP:760514647
195 195 -, at dbSNP:768546854
196 196 a, g dbSNP:142769650
201 201 a, g dbSNP:369894969
209 209 a, g dbSNP:776333344
211 211 a, g dbSNP:146980908
231 231 c, t dbSNP:764678212
239 239 a, g dbSNP:762424935
240 240 a, g dbSNP:768062115
241 241 c, t dbSNP:750824303
242 242 a, g dbSNP:138156448
248 248 c, t dbSNP:565843103
263 263 g, t dbSNP:200195383
268 268 c, t dbSNP:199794933
269 269 a, g dbSNP:372522881
272 272 c, t dbSNP:184779796
275 275 a, g dbSNP:756774414
276 276 c, t dbSNP:202080282
277 277 c, g dbSNP:537576399
285 285 c, t dbSNP:777913899
289 289 a, g dbSNP:376695433
292 292 c, t dbSNP:141766103
294 294 c, t dbSNP:200415936
297 297 c, t dbSNP:745710105
298 298 a, g dbSNP:111293783
300 300 a, g dbSNP:758768590
305 305 a, g dbSNP:557505985
319 319 a, t dbSNP:201117675
320 320 c, t dbSNP:746843611
329 329 c, t dbSNP:150593274
330 330 a, g dbSNP:375241929
338 338 c, t dbSNP:13589
342 342 c, t dbSNP:767035280
343 343 a, g dbSNP:374127769
352 352 a, g dbSNP:201076527
353 353 c, t dbSNP:139239258
359 359 a, g dbSNP:765494388
361 361 a, g dbSNP:752796010
370 370 c, t dbSNP:200236286
371 371 a, g dbSNP:371944933
377 377 -, g dbSNP:771396667
390 390 c, t dbSNP:553621405
391 391 a, g dbSNP:757162928
395 395 c, t dbSNP:781162398
396 396 a, g dbSNP:745655031
415 415 -, aga dbSNP:774717295
423 423 a, g dbSNP:535742062
435 435 a, g dbSNP:763432981
436 436 c, t dbSNP:764294751
442 442 a, c, g dbSNP:373421058
446 446 a, g dbSNP:767533462
461 461 a, g dbSNP:181540609
468 468 a, g dbSNP:755977099
472 472 g, t dbSNP:4784
473 473 c, t dbSNP:766393545
480 480 a, t dbSNP:16941677
481 481 a, g dbSNP:754888534
483 483 a, t dbSNP:549012048
490 490 a, c dbSNP:758099887
491 491 c, t dbSNP:777538559
508 508 c, t dbSNP:751027502
514 514 a, g dbSNP:756719485
523 523 a, g dbSNP:753462043
526 526 -, t dbSNP:767688792
532 532 a, g dbSNP:759136865
548 548 g, t dbSNP:10930
564 564 c, g dbSNP:770337731
576 576 a, g dbSNP:572701811
618 618 g, t dbSNP:752148801
655 655 a, c dbSNP:149130670
691 691 c, t dbSNP:561960570

Target ORF information:

RefSeq Version XM_011524839
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524839

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGAAGATCA CCTACGGCCA GTGCGGGGAT GTACTGCGGG CCCTGGGCCA GAACCCTACC 
AATGCCGAGG TGCTGCGTGT GCTGGGCAAG CCCAAGCCTG AAGAGATGAA TGTCAAGATG
CTGGACTTTG AGACGTTCTT GCCCATCCTG CAGCACATTT CCCGCAACAA GGAGCAGGGC
ACCTATGAGG ACTTCGTGGA GGGCCTGCGT GTCTTTGACA AGGAGAGCAA TGGCACGGTC
ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC ACCCTGGGAG AGAAGATGAC TGAGGCTGAA
GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT GCCAATGGCT GCATCAATTA TGAAGCCTTT
GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu30031
Accession Version NM_002476.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 594bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product myosin light chain 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X58851.1. On Dec 18, 2001 this sequence version replaced gi:4557037. Summary: Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (2) lacks the 5' exon but has an alternate 5' end, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC030228.1, BM919855.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_002467.1
CDS76..669
Misc Feature(1)220..663(+)
Misc Feature(2)271..273(+)
Misc Feature(3)475..660(+)
Misc Feature(4)502..642(+)
Misc Feature(5)661..663(+)
Exon (1)1..210
Gene:MYL4
Gene Synonym:
Exon (2)211..238
Gene:MYL4
Gene Synonym:
Exon (3)239..388
Gene:MYL4
Gene Synonym:
Exon (4)389..562
Gene:MYL4
Gene Synonym:
Exon (5)563..640
Gene:MYL4
Gene Synonym:
Exon (6)641..684
Gene:MYL4
Gene Synonym:
Exon (7)685..855
Gene:MYL4
Gene Synonym:
Translation
Position Chain Variation Link
16 16 c, t dbSNP:573384799
19 19 c, t dbSNP:574540052
21 21 c, t dbSNP:150616965
22 22 a, g dbSNP:111876061
26 26 c, g dbSNP:199619967
28 28 c, t dbSNP:758606614
29 29 a, g dbSNP:369748183
39 39 c, t dbSNP:1134983
45 45 a, g dbSNP:757348664
46 46 c, g, t dbSNP:781256182
48 48 c, t dbSNP:373169208
56 56 a, g dbSNP:775229902
67 67 c, t dbSNP:202127040
68 68 -, a dbSNP:773876585
70 70 a, g dbSNP:749072621
73 73 a, c dbSNP:370578383
90 90 g, t dbSNP:773929956
105 105 a, g dbSNP:140092425
113 113 a, c, t dbSNP:767067527
117 117 -, ccagct dbSNP:774642552
118 118 -, ccagctccagct dbSNP:763415247
118 118 -, ccagct dbSNP:766730973
122 122 c, g dbSNP:760072257
132 132 a, g dbSNP:765876288
139 139 g, t dbSNP:14793
140 140 c, t dbSNP:753082892
141 141 c, t dbSNP:201301071
143 143 c, g dbSNP:764371297
147 147 a, c, g dbSNP:370547497
161 161 c, t dbSNP:143784817
164 164 c, t dbSNP:781196896
174 174 -, ccc dbSNP:759787679
178 178 a, g dbSNP:750529340
184 184 a, c dbSNP:756010629
188 188 a, c, g, t dbSNP:780020430
190 190 -, t dbSNP:767743711
191 191 g, t dbSNP:778776213
194 194 a, t dbSNP:747700234
200 200 a, g dbSNP:771875522
203 203 a, g dbSNP:146860407
211 211 a, g dbSNP:140603047
225 225 c, t dbSNP:143234546
226 226 a, c, g dbSNP:772358836
228 228 c, g dbSNP:760839785
240 240 g, t dbSNP:1135060
241 241 g, t dbSNP:775677626
242 242 c, t dbSNP:144870368
245 245 a, c dbSNP:768696948
251 251 c, t dbSNP:199785200
254 254 c, t dbSNP:761664359
268 268 c, t dbSNP:767289051
275 275 c, t dbSNP:201579845
276 276 a, g dbSNP:149036573
280 280 a, g dbSNP:766060796
282 282 a, t dbSNP:753325178
283 283 a, g dbSNP:754540258
287 287 g, t dbSNP:527647806
294 294 c, g, t dbSNP:752046263
300 300 c, t dbSNP:372355856
301 301 a, g, t dbSNP:375596244
306 306 c, g, t dbSNP:143062804
308 308 a, g dbSNP:768793001
309 309 -, c dbSNP:776650813
309 309 c, t dbSNP:138710476
310 310 a, g dbSNP:748072025
312 312 a, g dbSNP:771938007
322 322 c, t dbSNP:773030593
323 323 a, g dbSNP:760514647
346 346 -, at dbSNP:768546854
347 347 a, g dbSNP:142769650
352 352 a, g dbSNP:369894969
360 360 a, g dbSNP:776333344
362 362 a, g dbSNP:146980908
382 382 c, t dbSNP:764678212
390 390 a, g dbSNP:762424935
391 391 a, g dbSNP:768062115
392 392 c, t dbSNP:750824303
393 393 a, g dbSNP:138156448
399 399 c, t dbSNP:565843103
414 414 g, t dbSNP:200195383
419 419 c, t dbSNP:199794933
420 420 a, g dbSNP:372522881
423 423 c, t dbSNP:184779796
426 426 a, g dbSNP:756774414
427 427 c, t dbSNP:202080282
428 428 c, g dbSNP:537576399
436 436 c, t dbSNP:777913899
440 440 a, g dbSNP:376695433
443 443 c, t dbSNP:141766103
445 445 c, t dbSNP:200415936
448 448 c, t dbSNP:745710105
449 449 a, g dbSNP:111293783
451 451 a, g dbSNP:758768590
456 456 a, g dbSNP:557505985
470 470 a, t dbSNP:201117675
471 471 c, t dbSNP:746843611
480 480 c, t dbSNP:150593274
481 481 a, g dbSNP:375241929
489 489 c, t dbSNP:13589
493 493 c, t dbSNP:767035280
494 494 a, g dbSNP:374127769
503 503 a, g dbSNP:201076527
504 504 c, t dbSNP:139239258
510 510 a, g dbSNP:765494388
512 512 a, g dbSNP:752796010
521 521 c, t dbSNP:200236286
522 522 a, g dbSNP:371944933
528 528 -, g dbSNP:771396667
541 541 c, t dbSNP:553621405
542 542 a, g dbSNP:757162928
546 546 c, t dbSNP:781162398
547 547 a, g dbSNP:745655031
566 566 -, aga dbSNP:774717295
574 574 a, g dbSNP:535742062
586 586 a, g dbSNP:763432981
587 587 c, t dbSNP:764294751
593 593 a, c, g dbSNP:373421058
597 597 a, g dbSNP:767533462
612 612 a, g dbSNP:181540609
619 619 a, g dbSNP:755977099
623 623 g, t dbSNP:4784
624 624 c, t dbSNP:766393545
631 631 a, t dbSNP:16941677
632 632 a, g dbSNP:754888534
634 634 a, t dbSNP:549012048
641 641 a, c dbSNP:758099887
642 642 c, t dbSNP:777538559
659 659 c, t dbSNP:751027502
665 665 a, g dbSNP:756719485
674 674 a, g dbSNP:753462043
677 677 -, t dbSNP:767688792
683 683 a, g dbSNP:759136865
699 699 g, t dbSNP:10930
715 715 c, g dbSNP:770337731
727 727 a, g dbSNP:572701811
769 769 g, t dbSNP:752148801
806 806 a, c dbSNP:149130670
842 842 c, t dbSNP:561960570

Target ORF information:

RefSeq Version NM_002476
Organism Homo sapiens (human)
Definition Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002476

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu30031
Accession Version NM_001002841.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 594bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product myosin light chain 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from H83803.1, BU658678.1 and BC030228.1. Summary: Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) represents the longer transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC030228.1, AF116676.2 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001002841.1
CDS129..722
Misc Feature(1)90..92(+)
Misc Feature(2)273..716(+)
Misc Feature(3)324..326(+)
Misc Feature(4)528..713(+)
Misc Feature(5)555..695(+)
Misc Feature(6)714..716(+)
Exon (1)1..94
Gene:MYL4
Gene Synonym:
Exon (2)95..263
Gene:MYL4
Gene Synonym:
Exon (3)264..291
Gene:MYL4
Gene Synonym:
Exon (4)292..441
Gene:MYL4
Gene Synonym:
Exon (5)442..615
Gene:MYL4
Gene Synonym:
Exon (6)616..693
Gene:MYL4
Gene Synonym:
Exon (7)694..737
Gene:MYL4
Gene Synonym:
Exon (8)738..908
Gene:MYL4
Gene Synonym:
Translation
Position Chain Variation Link
12 12 c, t dbSNP:568252930
40 40 c, t dbSNP:750184998
68 68 g, t dbSNP:537396263
98 98 a, g dbSNP:757348664
99 99 c, g, t dbSNP:781256182
101 101 c, t dbSNP:373169208
109 109 a, g dbSNP:775229902
120 120 c, t dbSNP:202127040
121 121 -, a dbSNP:773876585
123 123 a, g dbSNP:749072621
126 126 a, c dbSNP:370578383
143 143 g, t dbSNP:773929956
158 158 a, g dbSNP:140092425
166 166 a, c, t dbSNP:767067527
170 170 -, ccagct dbSNP:774642552
171 171 -, ccagctccagct dbSNP:763415247
171 171 -, ccagct dbSNP:766730973
175 175 c, g dbSNP:760072257
185 185 a, g dbSNP:765876288
192 192 g, t dbSNP:14793
193 193 c, t dbSNP:753082892
194 194 c, t dbSNP:201301071
196 196 c, g dbSNP:764371297
200 200 a, c, g dbSNP:370547497
214 214 c, t dbSNP:143784817
217 217 c, t dbSNP:781196896
227 227 -, ccc dbSNP:759787679
231 231 a, g dbSNP:750529340
237 237 a, c dbSNP:756010629
241 241 a, c, g, t dbSNP:780020430
243 243 -, t dbSNP:767743711
244 244 g, t dbSNP:778776213
247 247 a, t dbSNP:747700234
253 253 a, g dbSNP:771875522
256 256 a, g dbSNP:146860407
264 264 a, g dbSNP:140603047
278 278 c, t dbSNP:143234546
279 279 a, c, g dbSNP:772358836
281 281 c, g dbSNP:760839785
293 293 g, t dbSNP:1135060
294 294 g, t dbSNP:775677626
295 295 c, t dbSNP:144870368
298 298 a, c dbSNP:768696948
304 304 c, t dbSNP:199785200
307 307 c, t dbSNP:761664359
321 321 c, t dbSNP:767289051
328 328 c, t dbSNP:201579845
329 329 a, g dbSNP:149036573
333 333 a, g dbSNP:766060796
335 335 a, t dbSNP:753325178
336 336 a, g dbSNP:754540258
340 340 g, t dbSNP:527647806
347 347 c, g, t dbSNP:752046263
353 353 c, t dbSNP:372355856
354 354 a, g, t dbSNP:375596244
359 359 c, g, t dbSNP:143062804
361 361 a, g dbSNP:768793001
362 362 -, c dbSNP:776650813
362 362 c, t dbSNP:138710476
363 363 a, g dbSNP:748072025
365 365 a, g dbSNP:771938007
375 375 c, t dbSNP:773030593
376 376 a, g dbSNP:760514647
399 399 -, at dbSNP:768546854
400 400 a, g dbSNP:142769650
405 405 a, g dbSNP:369894969
413 413 a, g dbSNP:776333344
415 415 a, g dbSNP:146980908
435 435 c, t dbSNP:764678212
443 443 a, g dbSNP:762424935
444 444 a, g dbSNP:768062115
445 445 c, t dbSNP:750824303
446 446 a, g dbSNP:138156448
452 452 c, t dbSNP:565843103
467 467 g, t dbSNP:200195383
472 472 c, t dbSNP:199794933
473 473 a, g dbSNP:372522881
476 476 c, t dbSNP:184779796
479 479 a, g dbSNP:756774414
480 480 c, t dbSNP:202080282
481 481 c, g dbSNP:537576399
489 489 c, t dbSNP:777913899
493 493 a, g dbSNP:376695433
496 496 c, t dbSNP:141766103
498 498 c, t dbSNP:200415936
501 501 c, t dbSNP:745710105
502 502 a, g dbSNP:111293783
504 504 a, g dbSNP:758768590
509 509 a, g dbSNP:557505985
523 523 a, t dbSNP:201117675
524 524 c, t dbSNP:746843611
533 533 c, t dbSNP:150593274
534 534 a, g dbSNP:375241929
542 542 c, t dbSNP:13589
546 546 c, t dbSNP:767035280
547 547 a, g dbSNP:374127769
556 556 a, g dbSNP:201076527
557 557 c, t dbSNP:139239258
563 563 a, g dbSNP:765494388
565 565 a, g dbSNP:752796010
574 574 c, t dbSNP:200236286
575 575 a, g dbSNP:371944933
581 581 -, g dbSNP:771396667
594 594 c, t dbSNP:553621405
595 595 a, g dbSNP:757162928
599 599 c, t dbSNP:781162398
600 600 a, g dbSNP:745655031
619 619 -, aga dbSNP:774717295
627 627 a, g dbSNP:535742062
639 639 a, g dbSNP:763432981
640 640 c, t dbSNP:764294751
646 646 a, c, g dbSNP:373421058
650 650 a, g dbSNP:767533462
665 665 a, g dbSNP:181540609
672 672 a, g dbSNP:755977099
676 676 g, t dbSNP:4784
677 677 c, t dbSNP:766393545
684 684 a, t dbSNP:16941677
685 685 a, g dbSNP:754888534
687 687 a, t dbSNP:549012048
694 694 a, c dbSNP:758099887
695 695 c, t dbSNP:777538559
712 712 c, t dbSNP:751027502
718 718 a, g dbSNP:756719485
727 727 a, g dbSNP:753462043
730 730 -, t dbSNP:767688792
736 736 a, g dbSNP:759136865
752 752 g, t dbSNP:10930
768 768 c, g dbSNP:770337731
780 780 a, g dbSNP:572701811
822 822 g, t dbSNP:752148801
859 859 a, c dbSNP:149130670
895 895 c, t dbSNP:561960570

Target ORF information:

RefSeq Version NM_001002841
Organism Homo sapiens (human)
Definition Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001002841

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.