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NCAM1 neural cell adhesion molecule 1 [Homo sapiens (human)]

Gene Symbol NCAM1
Entrez Gene ID 4684
Full Name neural cell adhesion molecule 1
Synonyms CD56, MSK39, NCAM
General protein information
Preferred Names
neural cell adhesion molecule 1
Names
neural cell adhesion molecule 1
neural cell adhesion molecule, NCAM
antigen recognized by monoclonal antibody 5.1H11
NP_000606.3
NP_001070150.1
NP_001229536.1
NP_001229537.1
NP_851996.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11q23.1

Summary This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]. lac of sum
Disorder MIM:

116930

Disorder Html:





The following NCAM1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCAM1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu00262 NM_000615 Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 Quote Price
OHu20922 NM_181351 Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $379
OHu00364 NM_001076682 Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 Quote Price
OHu00345 NM_001242607 Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 Quote Price
OHu00771 NM_001242608 Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 Quote Price

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu00262
Accession Version NM_000615.6 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2547bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neural cell adhesion molecule 1 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from KF511120.1, DA337649.1, BC047244.1, S71824.1, BC014205.2, AK054570.1 and AW131780.1. On Jun 17, 2011 this sequence version replaced gi:117320538. Summary: This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]. Transcript Variant: This variant (1) lacks two alternate in-frame exons in the 3' coding region compared to variant 5. The resulting protein (isoform 1) is shorter compared to isoform 5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: S71824.1, U63041.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGA CTCTGGATGG GCACATGGTG
GTGCGTAGCC ATGCCCGTGT GTCGTCGCTG ACCCTGAAGA GCATCCAGTA CACTGATGCC
GGAGAGTACA TCTGCACCGC CAGCAACACC ATCGGCCAGG ACTCCCAGTC CATGTACCTT
GAAGTGCAAT ATGCCCCAAA GCTACAGGGC CCTGTGGCTG TGTACACTTG GGAGGGGAAC
CAGGTGAACA TCACCTGCGA GGTATTTGCC TATCCCAGTG CCACGATCTC ATGGTTTCGG
GATGGCCAGC TGCTGCCAAG CTCCAATTAC AGCAATATCA AGATCTACAA CACCCCCTCT
GCCAGCTATC TGGAGGTGAC CCCAGACTCT GAGAATGATT TTGGGAACTA CAACTGTACT
GCAGTGAACC GCATTGGGCA GGAGTCCTTG GAATTCATCC TTGTTCAAGC AGACACCCCC
TCTTCACCAT CCATCGACCA GGTGGAGCCA TACTCCAGCA CAGCCCAGGT GCAGTTTGAT
GAACCAGAGG CCACAGGTGG GGTGCCCATC CTCAAATACA AAGCTGAGTG GAGAGCAGTT
GGTGAAGAAG TATGGCATTC CAAGTGGTAT GATGCCAAGG AAGCCAGCAT GGAGGGCATC
GTCACCATCG TGGGCCTGAA GCCCGAAACA ACGTACGCCG TAAGGCTGGC GGCGCTCAAT
GGCAAAGGGC TGGGTGAGAT CAGCGCGGCC TCCGAGTTCA AGACGCAGCC AGTCCAAGGG
GAACCCAGTG CACCTAAGCT CGAAGGGCAG ATGGGAGAGG ATGGAAACTC TATTAAAGTG
AACCTGATCA AGCAGGATGA CGGCGGCTCC CCCATCAGAC ACTATCTGGT CAGGTACCGA
GCGCTCTCCT CCGAGTGGAA ACCAGAGATC AGGCTCCCGT CTGGCAGTGA CCACGTCATG
CTGAAGTCCC TGGACTGGAA TGCTGAGTAT GAGGTCTACG TGGTGGCTGA GAACCAGCAA
GGAAAATCCA AGGCGGCTCA TTTTGTGTTC AGGACCTCGG CCCAGCCCAC AGCCATCCCA
GCCAACGGCA GCCCCACCTC AGGCCTGAGC ACCGGGGCCA TCGTGGGCAT CCTCATCGTC
ATCTTCGTCC TGCTCCTGGT GGTTGTGGAC ATCACCTGCT ACTTCCTGAA CAAGTGTGGC
CTGTTCATGT GCATTGCGGT CAACCTGTGT GGAAAAGCCG GGCCCGGGGC CAAGGGCAAG
GACATGGAGG AGGGCAAGGC CGCCTTCTCG AAAGATGAGT CCAAGGAGCC CATCGTGGAG
GTTCGAACGG AGGAGGAGAG GACCCCAAAC CATGATGGAG GGAAACACAC AGAGCCCAAC
GAGACCACGC CACTGACGGA GCCCGAGAAG GGCCCCGTAG AAGCAAAGCC AGAGTGCCAG
GAGACAGAAA CGAAGCCAGC GCCAGCCGAA GTCAAGACGG TCCCCAATGA CGCCACACAG
ACAAAGGAGA ACGAGAGCAA AGCATGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000606.3
CDS367..2913
Misc Feature(1)424..711(+)
Misc Feature(2)427..702(+)
Misc Feature(3)469..480(+)
Misc Feature(4)757..942(+)
Misc Feature(5)769..936(+)
Misc Feature(6)997..1287(+)
Misc Feature(7)1030..1278(+)
Misc Feature(8)1282..1572(+)
Misc Feature(9)1333..1542(+)
Misc Feature(10)1597..1833(+)
Misc Feature(11)1630..1842(+)
Misc Feature(12)1711..1713(+)
Misc Feature(13)1798..1800(+)
Misc Feature(14)1858..2151(+)
Misc Feature(15)2068..2115(+)
Misc Feature(16)2116..2130(+)
Misc Feature(17)2212..2442(+)
Misc Feature(18)2362..2409(+)
Misc Feature(19)2410..2424(+)
Misc Feature(20)2491..2553(+)
Exon (1)1..418
Gene:NCAM1
Gene Synonym:
Exon (2)419..493
Gene:NCAM1
Gene Synonym:
Exon (3)494..712
Gene:NCAM1
Gene Synonym:
Exon (4)713..856
Gene:NCAM1
Gene Synonym:
Exon (5)857..994
Gene:NCAM1
Gene Synonym:
Exon (6)995..1112
Gene:NCAM1
Gene Synonym:
Exon (7)1113..1282
Gene:NCAM1
Gene Synonym:
Exon (8)1283..1425
Gene:NCAM1
Gene Synonym:
Exon (9)1426..1576
Gene:NCAM1
Gene Synonym:
Exon (10)1577..1761
Gene:NCAM1
Gene Synonym:
Exon (11)1762..1858
Gene:NCAM1
Gene Synonym:
Exon (12)1859..2029
Gene:NCAM1
Gene Synonym:
Exon (13)2030..2161
Gene:NCAM1
Gene Synonym:
Exon (14)2162..2164
Gene:NCAM1
Gene Synonym:
Exon (15)2165..2289
Gene:NCAM1
Gene Synonym:
Exon (16)2290..2467
Gene:NCAM1
Gene Synonym:
Exon (17)2468..2675
Gene:NCAM1
Gene Synonym:
Exon (18)2676..2792
Gene:NCAM1
Gene Synonym:
Exon (19)2793..5962
Gene:NCAM1
Gene Synonym:
Translation
Position Chain Variation Link
78 78 a, c, g dbSNP:7938749
313 313 a, c, g, t dbSNP:199895300
331 331 c, t dbSNP:201206514
372 372 -, g dbSNP:201772924
373 373 a, c dbSNP:112306738
385 385 a, c, t dbSNP:7105734
390 390 c, g, t dbSNP:6589347
402 402 a, t dbSNP:201161826
1036 1036 a, c dbSNP:199787286
1507 1507 c, g dbSNP:55757519
1510 1510 g, t dbSNP:56107970
1730 1730 a, t dbSNP:200123115
1986 1986 g, t dbSNP:584427
2188 2188 a, g dbSNP:746818916
2340 2340 c, t dbSNP:201880501
2482 2482 a, c dbSNP:201796555
2483 2483 c, g dbSNP:201646237
2499 2499 c, t dbSNP:35515801
2774 2774 c, t dbSNP:763253119
2977 2977 -, t dbSNP:60495668
3160 3160 a, g dbSNP:112708952
3681 3681 a, g dbSNP:12275130
3717 3717 a, g dbSNP:140002200
3929 3929 c, g dbSNP:75854397
4141 4141 a, g dbSNP:769486776
4196 4196 c, t dbSNP:200617376
4361 4361 a, g dbSNP:7927511
4363 4363 c, g dbSNP:7927811
4427 4427 c, t dbSNP:113237045
4980 4980 a, g dbSNP:111287313
5031 5031 a, g dbSNP:111990038
5279 5279 c, t dbSNP:12278611
5281 5281 c, t dbSNP:12278614
5296 5296 a, c dbSNP:12291713
5351 5351 c, t dbSNP:12278666
5372 5372 -, taaa dbSNP:151080039
5376 5376 a, t dbSNP:12793334
5400 5400 -, aaa dbSNP:386374947
5414 5414 a, t dbSNP:759841287
5416 5416 -, a dbSNP:765440959
5540 5540 a, g dbSNP:758170324
5589 5589 a, g dbSNP:751544935
5922 5922 c, t dbSNP:780744512

Target ORF information:

RefSeq Version NM_000615
Organism Homo sapiens (human)
Definition Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000615

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGA CTCTGGATGG GCACATGGTG
GTGCGTAGCC ATGCCCGTGT GTCGTCGCTG ACCCTGAAGA GCATCCAGTA CACTGATGCC
GGAGAGTACA TCTGCACCGC CAGCAACACC ATCGGCCAGG ACTCCCAGTC CATGTACCTT
GAAGTGCAAT ATGCCCCAAA GCTACAGGGC CCTGTGGCTG TGTACACTTG GGAGGGGAAC
CAGGTGAACA TCACCTGCGA GGTATTTGCC TATCCCAGTG CCACGATCTC ATGGTTTCGG
GATGGCCAGC TGCTGCCAAG CTCCAATTAC AGCAATATCA AGATCTACAA CACCCCCTCT
GCCAGCTATC TGGAGGTGAC CCCAGACTCT GAGAATGATT TTGGGAACTA CAACTGTACT
GCAGTGAACC GCATTGGGCA GGAGTCCTTG GAATTCATCC TTGTTCAAGC AGACACCCCC
TCTTCACCAT CCATCGACCA GGTGGAGCCA TACTCCAGCA CAGCCCAGGT GCAGTTTGAT
GAACCAGAGG CCACAGGTGG GGTGCCCATC CTCAAATACA AAGCTGAGTG GAGAGCAGTT
GGTGAAGAAG TATGGCATTC CAAGTGGTAT GATGCCAAGG AAGCCAGCAT GGAGGGCATC
GTCACCATCG TGGGCCTGAA GCCCGAAACA ACGTACGCCG TAAGGCTGGC GGCGCTCAAT
GGCAAAGGGC TGGGTGAGAT CAGCGCGGCC TCCGAGTTCA AGACGCAGCC AGTCCAAGGG
GAACCCAGTG CACCTAAGCT CGAAGGGCAG ATGGGAGAGG ATGGAAACTC TATTAAAGTG
AACCTGATCA AGCAGGATGA CGGCGGCTCC CCCATCAGAC ACTATCTGGT CAGGTACCGA
GCGCTCTCCT CCGAGTGGAA ACCAGAGATC AGGCTCCCGT CTGGCAGTGA CCACGTCATG
CTGAAGTCCC TGGACTGGAA TGCTGAGTAT GAGGTCTACG TGGTGGCTGA GAACCAGCAA
GGAAAATCCA AGGCGGCTCA TTTTGTGTTC AGGACCTCGG CCCAGCCCAC AGCCATCCCA
GCCAACGGCA GCCCCACCTC AGGCCTGAGC ACCGGGGCCA TCGTGGGCAT CCTCATCGTC
ATCTTCGTCC TGCTCCTGGT GGTTGTGGAC ATCACCTGCT ACTTCCTGAA CAAGTGTGGC
CTGTTCATGT GCATTGCGGT CAACCTGTGT GGAAAAGCCG GGCCCGGGGC CAAGGGCAAG
GACATGGAGG AGGGCAAGGC CGCCTTCTCG AAAGATGAGT CCAAGGAGCC CATCGTGGAG
GTTCGAACGG AGGAGGAGAG GACCCCAAAC CATGATGGAG GGAAACACAC AGAGCCCAAC
GAGACCACGC CACTGACGGA GCCCGAGAAG GGCCCCGTAG AAGCAAAGCC AGAGTGCCAG
GAGACAGAAA CGAAGCCAGC GCCAGCCGAA GTCAAGACGG TCCCCAATGA CGCCACACAG
ACAAAGGAGA ACGAGAGCAA AGCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20922
Accession Version NM_181351.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neural cell adhesion molecule 1 isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from KF511120.1, DA337649.1, BC047244.1, S71824.1, BC014205.2, AK054570.1 and AW131780.1. This sequence is a reference standard in the RefSeqGene project. On Jun 17, 2011 this sequence version replaced gi:117320546. Summary: This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region compared to variant 5. The resulting protein (isoform 2) is shorter compared to isoform 5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC047244.1, AK292453.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGG CTTCGTGGAC TCGACCAGAG
AAGCAAGAGA CTCTGGATGG GCACATGGTG GTGCGTAGCC ATGCCCGTGT GTCGTCGCTG
ACCCTGAAGA GCATCCAGTA CACTGATGCC GGAGAGTACA TCTGCACCGC CAGCAACACC
ATCGGCCAGG ACTCCCAGTC CATGTACCTT GAAGTGCAAT ATGCCCCAAA GCTACAGGGC
CCTGTGGCTG TGTACACTTG GGAGGGGAAC CAGGTGAACA TCACCTGCGA GGTATTTGCC
TATCCCAGTG CCACGATCTC ATGGTTTCGG GATGGCCAGC TGCTGCCAAG CTCCAATTAC
AGCAATATCA AGATCTACAA CACCCCCTCT GCCAGCTATC TGGAGGTGAC CCCAGACTCT
GAGAATGATT TTGGGAACTA CAACTGTACT GCAGTGAACC GCATTGGGCA GGAGTCCTTG
GAATTCATCC TTGTTCAAGC AGACACCCCC TCTTCACCAT CCATCGACCA GGTGGAGCCA
TACTCCAGCA CAGCCCAGGT GCAGTTTGAT GAACCAGAGG CCACAGGTGG GGTGCCCATC
CTCAAATACA AAGCTGAGTG GAGAGCAGTT GGTGAAGAAG TATGGCATTC CAAGTGGTAT
GATGCCAAGG AAGCCAGCAT GGAGGGCATC GTCACCATCG TGGGCCTGAA GCCCGAAACA
ACGTACGCCG TAAGGCTGGC GGCGCTCAAT GGCAAAGGGC TGGGTGAGAT CAGCGCGGCC
TCCGAGTTCA AGACGCAGCC AGTCCAAGGG GAACCCAGTG CACCTAAGCT CGAAGGGCAG
ATGGGAGAGG ATGGAAACTC TATTAAAGTG AACCTGATCA AGCAGGATGA CGGCGGCTCC
CCCATCAGAC ACTATCTGGT CAGGTACCGA GCGCTCTCCT CCGAGTGGAA ACCAGAGATC
AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTGAAGTCCC TGGACTGGAA TGCTGAGTAT
GAGGTCTACG TGGTGGCTGA GAACCAGCAA GGAAAATCCA AGGCGGCTCA TTTTGTGTTC
AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC AGGCCTGAGC
ACCGGGGCCA TCGTGGGCAT CCTCATCGTC ATCTTCGTCC TGCTCCTGGT GGTTGTGGAC
ATCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGTTCATGT GCATTGCGGT CAACCTGTGT
GGAAAAGCCG GGCCCGGGGC CAAGGGCAAG GACATGGAGG AGGGCAAGGC CGCCTTCTCG
AAAGATGAGT CCAAGGAGCC CATCGTGGAG GTTCGAACGG AGGAGGAGAG GACCCCAAAC
CATGATGGAG GGAAACACAC AGAGCCCAAC GAGACCACGC CACTGACGGA GCCCGAGAAG
GGCCCCGTAG AAGCAAAGCC AGAGTGCCAG GAGACAGAAA CGAAGCCAGC GCCAGCCGAA
GTCAAGACGG TCCCCAATGA CGCCACACAG ACAAAGGAGA ACGAGAGCAA AGCATGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_851996.2
CDS367..2943
Misc Feature(1)424..711(+)
Misc Feature(2)427..702(+)
Misc Feature(3)469..480(+)
Misc Feature(4)757..942(+)
Misc Feature(5)769..936(+)
Misc Feature(6)997..1287(+)
Misc Feature(7)1030..1278(+)
Misc Feature(8)1282..1602(+)
Misc Feature(9)1333..1572(+)
Misc Feature(10)1627..1863(+)
Misc Feature(11)1660..1872(+)
Misc Feature(12)1741..1743(+)
Misc Feature(13)1828..1830(+)
Misc Feature(14)1888..2181(+)
Misc Feature(15)2098..2145(+)
Misc Feature(16)2146..2160(+)
Misc Feature(17)2242..2472(+)
Misc Feature(18)2392..2439(+)
Misc Feature(19)2440..2454(+)
Misc Feature(20)2521..2583(+)
Exon (1)1..418
Gene:NCAM1
Gene Synonym:
Exon (2)419..493
Gene:NCAM1
Gene Synonym:
Exon (3)494..712
Gene:NCAM1
Gene Synonym:
Exon (4)713..856
Gene:NCAM1
Gene Synonym:
Exon (5)857..994
Gene:NCAM1
Gene Synonym:
Exon (6)995..1112
Gene:NCAM1
Gene Synonym:
Exon (7)1113..1282
Gene:NCAM1
Gene Synonym:
Exon (8)1283..1425
Gene:NCAM1
Gene Synonym:
Exon (9)1426..1455
Gene:NCAM1
Gene Synonym:
Exon (10)1456..1606
Gene:NCAM1
Gene Synonym:
Exon (11)1607..1791
Gene:NCAM1
Gene Synonym:
Exon (12)1792..1888
Gene:NCAM1
Gene Synonym:
Exon (13)1889..2059
Gene:NCAM1
Gene Synonym:
Exon (14)2060..2191
Gene:NCAM1
Gene Synonym:
Exon (15)2192..2194
Gene:NCAM1
Gene Synonym:
Exon (16)2195..2319
Gene:NCAM1
Gene Synonym:
Exon (17)2320..2497
Gene:NCAM1
Gene Synonym:
Exon (18)2498..2705
Gene:NCAM1
Gene Synonym:
Exon (19)2706..2822
Gene:NCAM1
Gene Synonym:
Exon (20)2823..5992
Gene:NCAM1
Gene Synonym:
Translation
Position Chain Variation Link
78 78 a, c, g dbSNP:7938749
313 313 a, c, g, t dbSNP:199895300
331 331 c, t dbSNP:201206514
372 372 -, g dbSNP:201772924
373 373 a, c dbSNP:112306738
385 385 a, c, t dbSNP:7105734
390 390 c, g, t dbSNP:6589347
402 402 a, t dbSNP:201161826
1036 1036 a, c dbSNP:199787286
1537 1537 c, g dbSNP:55757519
1540 1540 g, t dbSNP:56107970
1760 1760 a, t dbSNP:200123115
2016 2016 g, t dbSNP:584427
2218 2218 a, g dbSNP:746818916
2370 2370 c, t dbSNP:201880501
2512 2512 a, c dbSNP:201796555
2513 2513 c, g dbSNP:201646237
2529 2529 c, t dbSNP:35515801
2804 2804 c, t dbSNP:763253119
3007 3007 -, t dbSNP:60495668
3190 3190 a, g dbSNP:112708952
3711 3711 a, g dbSNP:12275130
3747 3747 a, g dbSNP:140002200
3959 3959 c, g dbSNP:75854397
4171 4171 a, g dbSNP:769486776
4226 4226 c, t dbSNP:200617376
4391 4391 a, g dbSNP:7927511
4393 4393 c, g dbSNP:7927811
4457 4457 c, t dbSNP:113237045
5010 5010 a, g dbSNP:111287313
5061 5061 a, g dbSNP:111990038
5309 5309 c, t dbSNP:12278611
5311 5311 c, t dbSNP:12278614
5326 5326 a, c dbSNP:12291713
5381 5381 c, t dbSNP:12278666
5402 5402 -, taaa dbSNP:151080039
5406 5406 a, t dbSNP:12793334
5430 5430 -, aaa dbSNP:386374947
5444 5444 a, t dbSNP:759841287
5446 5446 -, a dbSNP:765440959
5570 5570 a, g dbSNP:758170324
5619 5619 a, g dbSNP:751544935
5952 5952 c, t dbSNP:780744512

Target ORF information:

RefSeq Version NM_181351
Organism Homo sapiens (human)
Definition Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_181351

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGG CTTCGTGGAC TCGACCAGAG
AAGCAAGAGA CTCTGGATGG GCACATGGTG GTGCGTAGCC ATGCCCGTGT GTCGTCGCTG
ACCCTGAAGA GCATCCAGTA CACTGATGCC GGAGAGTACA TCTGCACCGC CAGCAACACC
ATCGGCCAGG ACTCCCAGTC CATGTACCTT GAAGTGCAAT ATGCCCCAAA GCTACAGGGC
CCTGTGGCTG TGTACACTTG GGAGGGGAAC CAGGTGAACA TCACCTGCGA GGTATTTGCC
TATCCCAGTG CCACGATCTC ATGGTTTCGG GATGGCCAGC TGCTGCCAAG CTCCAATTAC
AGCAATATCA AGATCTACAA CACCCCCTCT GCCAGCTATC TGGAGGTGAC CCCAGACTCT
GAGAATGATT TTGGGAACTA CAACTGTACT GCAGTGAACC GCATTGGGCA GGAGTCCTTG
GAATTCATCC TTGTTCAAGC AGACACCCCC TCTTCACCAT CCATCGACCA GGTGGAGCCA
TACTCCAGCA CAGCCCAGGT GCAGTTTGAT GAACCAGAGG CCACAGGTGG GGTGCCCATC
CTCAAATACA AAGCTGAGTG GAGAGCAGTT GGTGAAGAAG TATGGCATTC CAAGTGGTAT
GATGCCAAGG AAGCCAGCAT GGAGGGCATC GTCACCATCG TGGGCCTGAA GCCCGAAACA
ACGTACGCCG TAAGGCTGGC GGCGCTCAAT GGCAAAGGGC TGGGTGAGAT CAGCGCGGCC
TCCGAGTTCA AGACGCAGCC AGTCCAAGGG GAACCCAGTG CACCTAAGCT CGAAGGGCAG
ATGGGAGAGG ATGGAAACTC TATTAAAGTG AACCTGATCA AGCAGGATGA CGGCGGCTCC
CCCATCAGAC ACTATCTGGT CAGGTACCGA GCGCTCTCCT CCGAGTGGAA ACCAGAGATC
AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTGAAGTCCC TGGACTGGAA TGCTGAGTAT
GAGGTCTACG TGGTGGCTGA GAACCAGCAA GGAAAATCCA AGGCGGCTCA TTTTGTGTTC
AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC AGGCCTGAGC
ACCGGGGCCA TCGTGGGCAT CCTCATCGTC ATCTTCGTCC TGCTCCTGGT GGTTGTGGAC
ATCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGTTCATGT GCATTGCGGT CAACCTGTGT
GGAAAAGCCG GGCCCGGGGC CAAGGGCAAG GACATGGAGG AGGGCAAGGC CGCCTTCTCG
AAAGATGAGT CCAAGGAGCC CATCGTGGAG GTTCGAACGG AGGAGGAGAG GACCCCAAAC
CATGATGGAG GGAAACACAC AGAGCCCAAC GAGACCACGC CACTGACGGA GCCCGAGAAG
GGCCCCGTAG AAGCAAAGCC AGAGTGCCAG GAGACAGAAA CGAAGCCAGC GCCAGCCGAA
GTCAAGACGG TCCCCAATGA CGCCACACAG ACAAAGGAGA ACGAGAGCAA AGCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00364
Accession Version NM_001076682.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neural cell adhesion molecule 1 isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from KF511120.1, DA337649.1, BC047244.1, S71824.1, X16841.1, AB209443.1 and BC019845.1. On Jun 17, 2011 this sequence version replaced gi:117320541. Summary: This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]. Transcript Variant: This variant (3) has multiple differences in the coding region and uses an alternate splice site in the 3' coding region compared to variant 5. The resulting protein (isoform 3) is shorter and has a distinct C-terminus compared to isoform 5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X16841.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGA CTCTGGATGG GCACATGGTG
GTGCGTAGCC ATGCCCGTGT GTCGTCGCTG ACCCTGAAGA GCATCCAGTA CACTGATGCC
GGAGAGTACA TCTGCACCGC CAGCAACACC ATCGGCCAGG ACTCCCAGTC CATGTACCTT
GAAGTGCAAT ATGCCCCAAA GCTACAGGGC CCTGTGGCTG TGTACACTTG GGAGGGGAAC
CAGGTGAACA TCACCTGCGA GGTATTTGCC TATCCCAGTG CCACGATCTC ATGGTTTCGG
GATGGCCAGC TGCTGCCAAG CTCCAATTAC AGCAATATCA AGATCTACAA CACCCCCTCT
GCCAGCTATC TGGAGGTGAC CCCAGACTCT GAGAATGATT TTGGGAACTA CAACTGTACT
GCAGTGAACC GCATTGGGCA GGAGTCCTTG GAATTCATCC TTGTTCAAGC AGACACCCCC
TCTTCACCAT CCATCGACCA GGTGGAGCCA TACTCCAGCA CAGCCCAGGT GCAGTTTGAT
GAACCAGAGG CCACAGGTGG GGTGCCCATC CTCAAATACA AAGCTGAGTG GAGAGCAGTT
GGTGAAGAAG TATGGCATTC CAAGTGGTAT GATGCCAAGG AAGCCAGCAT GGAGGGCATC
GTCACCATCG TGGGCCTGAA GCCCGAAACA ACGTACGCCG TAAGGCTGGC GGCGCTCAAT
GGCAAAGGGC TGGGTGAGAT CAGCGCGGCC TCCGAGTTCA AGACGCAGCC AGTCCATAGC
CCTCCTCCAC CGGCATCTGC TAGCTCGTCT ACCCCTGTTC CATTGTCTCC ACCAGATACA
ACTTGGCCTC TTCCTGCCCT TGCAACCACA GAACCAGCTA AAGGGGAACC CAGTGCACCT
AAGCTCGAAG GGCAGATGGG AGAGGATGGA AACTCTATTA AAGTGAACCT GATCAAGCAG
GATGACGGCG GCTCCCCCAT CAGACACTAT CTGGTCAGGT ACCGAGCGCT CTCCTCCGAG
TGGAAACCAG AGATCAGGCT CCCGTCTGGC AGTGACCACG TCATGCTGAA GTCCCTGGAC
TGGAATGCTG AGTATGAGGT CTACGTGGTG GCTGAGAACC AGCAAGGAAA ATCCAAGGCG
GCTCATTTTG TGTTCAGGAC CTCGGCCCAG CCCACAGCCA TCCCAGCAAC CTTGGGAGGC
AATTCTGCAT CCTACACCTT TGTCTCATTG CTTTTCTCTG CAGTGACTCT TCTTTTGCTC
TGTTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001070150.1
CDS367..2652
Misc Feature(1)424..711(+)
Misc Feature(2)427..702(+)
Misc Feature(3)469..480(+)
Misc Feature(4)757..942(+)
Misc Feature(5)769..936(+)
Misc Feature(6)997..1287(+)
Misc Feature(7)1030..1278(+)
Misc Feature(8)1282..1572(+)
Misc Feature(9)1333..1542(+)
Misc Feature(10)1618..1824(+)
Misc Feature(11)1630..1842(+)
Misc Feature(12)1858..2151(+)
Misc Feature(13)2068..2115(+)
Misc Feature(14)2116..2130(+)
Misc Feature(15)2317..2547(+)
Misc Feature(16)2467..2514(+)
Misc Feature(17)2515..2529(+)
Exon (1)1..418
Gene:NCAM1
Gene Synonym:
Exon (2)419..493
Gene:NCAM1
Gene Synonym:
Exon (3)494..712
Gene:NCAM1
Gene Synonym:
Exon (4)713..856
Gene:NCAM1
Gene Synonym:
Exon (5)857..994
Gene:NCAM1
Gene Synonym:
Exon (6)995..1112
Gene:NCAM1
Gene Synonym:
Exon (7)1113..1282
Gene:NCAM1
Gene Synonym:
Exon (8)1283..1425
Gene:NCAM1
Gene Synonym:
Exon (9)1426..1576
Gene:NCAM1
Gene Synonym:
Exon (10)1577..1761
Gene:NCAM1
Gene Synonym:
Exon (11)1762..1858
Gene:NCAM1
Gene Synonym:
Exon (12)1859..2029
Gene:NCAM1
Gene Synonym:
Exon (13)2030..2161
Gene:NCAM1
Gene Synonym:
Exon (14)2162..2176
Gene:NCAM1
Gene Synonym:
Exon (15)2177..2224
Gene:NCAM1
Gene Synonym:
Exon (16)2225..2266
Gene:NCAM1
Gene Synonym:
Exon (17)2267..2269
Gene:NCAM1
Gene Synonym:
Exon (18)2270..2394
Gene:NCAM1
Gene Synonym:
Exon (19)2395..2572
Gene:NCAM1
Gene Synonym:
Exon (20)2573..4924
Gene:NCAM1
Gene Synonym:
Translation
Position Chain Variation Link
78 78 a, c, g dbSNP:7938749
313 313 a, c, g, t dbSNP:199895300
331 331 c, t dbSNP:201206514
372 372 -, g dbSNP:201772924
373 373 a, c dbSNP:112306738
385 385 a, c, t dbSNP:7105734
390 390 c, g, t dbSNP:6589347
402 402 a, t dbSNP:201161826
1036 1036 a, c dbSNP:199787286
1507 1507 c, g dbSNP:55757519
1510 1510 g, t dbSNP:56107970
1730 1730 a, t dbSNP:200123115
1986 1986 g, t dbSNP:584427
2293 2293 a, g dbSNP:746818916
2445 2445 c, t dbSNP:201880501
2681 2681 g, t dbSNP:2288158
2729 2729 -, atgg dbSNP:146542910
2780 2780 a, g dbSNP:188136769
2928 2928 c, t dbSNP:768824649
3330 3330 g, t dbSNP:762082614
3400 3400 a, g dbSNP:767723581
3610 3610 a, g dbSNP:2298703
3620 3620 a, g dbSNP:7946128
3823 3823 c, t dbSNP:10502171
4011 4011 c, t dbSNP:1006826
4208 4208 c, t dbSNP:72995546
4287 4287 g, t dbSNP:777506545
4288 4288 a, t dbSNP:746254054
4335 4335 c, t dbSNP:625741
4538 4538 a, g dbSNP:111648733
4600 4600 a, g dbSNP:769033510
4784 4784 a, c dbSNP:748160487
4871 4871 c, g dbSNP:654362
4917 4917 c, t dbSNP:766244760

Target ORF information:

RefSeq Version NM_001076682
Organism Homo sapiens (human)
Definition Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001076682

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGA CTCTGGATGG GCACATGGTG
GTGCGTAGCC ATGCCCGTGT GTCGTCGCTG ACCCTGAAGA GCATCCAGTA CACTGATGCC
GGAGAGTACA TCTGCACCGC CAGCAACACC ATCGGCCAGG ACTCCCAGTC CATGTACCTT
GAAGTGCAAT ATGCCCCAAA GCTACAGGGC CCTGTGGCTG TGTACACTTG GGAGGGGAAC
CAGGTGAACA TCACCTGCGA GGTATTTGCC TATCCCAGTG CCACGATCTC ATGGTTTCGG
GATGGCCAGC TGCTGCCAAG CTCCAATTAC AGCAATATCA AGATCTACAA CACCCCCTCT
GCCAGCTATC TGGAGGTGAC CCCAGACTCT GAGAATGATT TTGGGAACTA CAACTGTACT
GCAGTGAACC GCATTGGGCA GGAGTCCTTG GAATTCATCC TTGTTCAAGC AGACACCCCC
TCTTCACCAT CCATCGACCA GGTGGAGCCA TACTCCAGCA CAGCCCAGGT GCAGTTTGAT
GAACCAGAGG CCACAGGTGG GGTGCCCATC CTCAAATACA AAGCTGAGTG GAGAGCAGTT
GGTGAAGAAG TATGGCATTC CAAGTGGTAT GATGCCAAGG AAGCCAGCAT GGAGGGCATC
GTCACCATCG TGGGCCTGAA GCCCGAAACA ACGTACGCCG TAAGGCTGGC GGCGCTCAAT
GGCAAAGGGC TGGGTGAGAT CAGCGCGGCC TCCGAGTTCA AGACGCAGCC AGTCCATAGC
CCTCCTCCAC CGGCATCTGC TAGCTCGTCT ACCCCTGTTC CATTGTCTCC ACCAGATACA
ACTTGGCCTC TTCCTGCCCT TGCAACCACA GAACCAGCTA AAGGGGAACC CAGTGCACCT
AAGCTCGAAG GGCAGATGGG AGAGGATGGA AACTCTATTA AAGTGAACCT GATCAAGCAG
GATGACGGCG GCTCCCCCAT CAGACACTAT CTGGTCAGGT ACCGAGCGCT CTCCTCCGAG
TGGAAACCAG AGATCAGGCT CCCGTCTGGC AGTGACCACG TCATGCTGAA GTCCCTGGAC
TGGAATGCTG AGTATGAGGT CTACGTGGTG GCTGAGAACC AGCAAGGAAA ATCCAAGGCG
GCTCATTTTG TGTTCAGGAC CTCGGCCCAG CCCACAGCCA TCCCAGCAAC CTTGGGAGGC
AATTCTGCAT CCTACACCTT TGTCTCATTG CTTTTCTCTG CAGTGACTCT TCTTTTGCTC
TGTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00345
Accession Version NM_001242607.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2655bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neural cell adhesion molecule 1 isoform 5 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from KF511120.1, AK314589.1, AP002853.4 and AP000880.4. Summary: This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]. Transcript Variant: This variant (5) represents the longest transcript and encodes the longest protein (isoform 5). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314589.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGG CTTCGTGGAC TCGACCAGAG
AAGCAAGAGG TACATGCACC ATGGAACTGG CAAGTGGGCA GACAGAAAGG ACAGGCTGGC
AGTGCAGGTT TCCCAGGATC TCATGAGACT CTGGATGGGC ACATGGTGGT GCGTAGCCAT
GCCCGTGTGT CGTCGCTGAC CCTGAAGAGC ATCCAGTACA CTGATGCCGG AGAGTACATC
TGCACCGCCA GCAACACCAT CGGCCAGGAC TCCCAGTCCA TGTACCTTGA AGTGCAATAT
GCCCCAAAGC TACAGGGCCC TGTGGCTGTG TACACTTGGG AGGGGAACCA GGTGAACATC
ACCTGCGAGG TATTTGCCTA TCCCAGTGCC ACGATCTCAT GGTTTCGGGA TGGCCAGCTG
CTGCCAAGCT CCAATTACAG CAATATCAAG ATCTACAACA CCCCCTCTGC CAGCTATCTG
GAGGTGACCC CAGACTCTGA GAATGATTTT GGGAACTACA ACTGTACTGC AGTGAACCGC
ATTGGGCAGG AGTCCTTGGA ATTCATCCTT GTTCAAGCAG ACACCCCCTC TTCACCATCC
ATCGACCAGG TGGAGCCATA CTCCAGCACA GCCCAGGTGC AGTTTGATGA ACCAGAGGCC
ACAGGTGGGG TGCCCATCCT CAAATACAAA GCTGAGTGGA GAGCAGTTGG TGAAGAAGTA
TGGCATTCCA AGTGGTATGA TGCCAAGGAA GCCAGCATGG AGGGCATCGT CACCATCGTG
GGCCTGAAGC CCGAAACAAC GTACGCCGTA AGGCTGGCGG CGCTCAATGG CAAAGGGCTG
GGTGAGATCA GCGCGGCCTC CGAGTTCAAG ACGCAGCCAG TCCAAGGGGA ACCCAGTGCA
CCTAAGCTCG AAGGGCAGAT GGGAGAGGAT GGAAACTCTA TTAAAGTGAA CCTGATCAAG
CAGGATGACG GCGGCTCCCC CATCAGACAC TATCTGGTCA GGTACCGAGC GCTCTCCTCC
GAGTGGAAAC CAGAGATCAG GCTCCCGTCT GGCAGTGACC ACGTCATGCT GAAGTCCCTG
GACTGGAATG CTGAGTATGA GGTCTACGTG GTGGCTGAGA ACCAGCAAGG AAAATCCAAG
GCGGCTCATT TTGTGTTCAG GACCTCGGCC CAGCCCACAG CCATCCCAGC CAACGGCAGC
CCCACCTCAG GCCTGAGCAC CGGGGCCATC GTGGGCATCC TCATCGTCAT CTTCGTCCTG
CTCCTGGTGG TTGTGGACAT CACCTGCTAC TTCCTGAACA AGTGTGGCCT GTTCATGTGC
ATTGCGGTCA ACCTGTGTGG AAAAGCCGGG CCCGGGGCCA AGGGCAAGGA CATGGAGGAG
GGCAAGGCCG CCTTCTCGAA AGATGAGTCC AAGGAGCCCA TCGTGGAGGT TCGAACGGAG
GAGGAGAGGA CCCCAAACCA TGATGGAGGG AAACACACAG AGCCCAACGA GACCACGCCA
CTGACGGAGC CCGAGAAGGG CCCCGTAGAA GCAAAGCCAG AGTGCCAGGA GACAGAAACG
AAGCCAGCGC CAGCCGAAGT CAAGACGGTC CCCAATGACG CCACACAGAC AAAGGAGAAC
GAGAGCAAAG CATGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001229536.1
CDS367..3021
Misc Feature(1)424..711(+)
Misc Feature(2)427..702(+)
Misc Feature(3)469..480(+)
Misc Feature(4)757..942(+)
Misc Feature(5)769..936(+)
Misc Feature(6)997..1287(+)
Misc Feature(7)1030..1278(+)
Misc Feature(8)1282..1680(+)
Misc Feature(9)1333..1650(+)
Misc Feature(10)1705..1941(+)
Misc Feature(11)1738..1950(+)
Misc Feature(12)1966..2259(+)
Misc Feature(13)2176..2223(+)
Misc Feature(14)2224..2238(+)
Misc Feature(15)2320..2550(+)
Misc Feature(16)2470..2517(+)
Misc Feature(17)2518..2532(+)
Exon (1)1..418
Gene:NCAM1
Gene Synonym:
Exon (2)419..493
Gene:NCAM1
Gene Synonym:
Exon (3)494..712
Gene:NCAM1
Gene Synonym:
Exon (4)713..856
Gene:NCAM1
Gene Synonym:
Exon (5)857..994
Gene:NCAM1
Gene Synonym:
Exon (6)995..1112
Gene:NCAM1
Gene Synonym:
Exon (7)1113..1282
Gene:NCAM1
Gene Synonym:
Exon (8)1283..1425
Gene:NCAM1
Gene Synonym:
Exon (9)1426..1455
Gene:NCAM1
Gene Synonym:
Exon (10)1456..1533
Gene:NCAM1
Gene Synonym:
Exon (11)1534..1684
Gene:NCAM1
Gene Synonym:
Exon (12)1685..1869
Gene:NCAM1
Gene Synonym:
Exon (13)1870..1966
Gene:NCAM1
Gene Synonym:
Exon (14)1967..2137
Gene:NCAM1
Gene Synonym:
Exon (15)2138..2269
Gene:NCAM1
Gene Synonym:
Exon (16)2270..2272
Gene:NCAM1
Gene Synonym:
Exon (17)2273..2397
Gene:NCAM1
Gene Synonym:
Exon (18)2398..2575
Gene:NCAM1
Gene Synonym:
Exon (19)2576..2783
Gene:NCAM1
Gene Synonym:
Exon (20)2784..2900
Gene:NCAM1
Gene Synonym:
Exon (21)2901..6070
Gene:NCAM1
Gene Synonym:
Translation
Position Chain Variation Link
78 78 a, c, g dbSNP:7938749
313 313 a, c, g, t dbSNP:199895300
331 331 c, t dbSNP:201206514
372 372 -, g dbSNP:201772924
373 373 a, c dbSNP:112306738
385 385 a, c, t dbSNP:7105734
390 390 c, g, t dbSNP:6589347
402 402 a, t dbSNP:201161826
1036 1036 a, c dbSNP:199787286
1495 1495 a, g dbSNP:267602694
1615 1615 c, g dbSNP:55757519
1618 1618 g, t dbSNP:56107970
1838 1838 a, t dbSNP:200123115
2094 2094 g, t dbSNP:584427
2296 2296 a, g dbSNP:746818916
2448 2448 c, t dbSNP:201880501
2590 2590 a, c dbSNP:201796555
2591 2591 c, g dbSNP:201646237
2607 2607 c, t dbSNP:35515801
2882 2882 c, t dbSNP:763253119
3085 3085 -, t dbSNP:60495668
3268 3268 a, g dbSNP:112708952
3789 3789 a, g dbSNP:12275130
3825 3825 a, g dbSNP:140002200
4037 4037 c, g dbSNP:75854397
4249 4249 a, g dbSNP:769486776
4304 4304 c, t dbSNP:200617376
4469 4469 a, g dbSNP:7927511
4471 4471 c, g dbSNP:7927811
4535 4535 c, t dbSNP:113237045
5088 5088 a, g dbSNP:111287313
5139 5139 a, g dbSNP:111990038
5387 5387 c, t dbSNP:12278611
5389 5389 c, t dbSNP:12278614
5404 5404 a, c dbSNP:12291713
5459 5459 c, t dbSNP:12278666
5480 5480 -, taaa dbSNP:151080039
5484 5484 a, t dbSNP:12793334
5508 5508 -, aaa dbSNP:386374947
5522 5522 a, t dbSNP:759841287
5524 5524 -, a dbSNP:765440959
5648 5648 a, g dbSNP:758170324
5697 5697 a, g dbSNP:751544935
6030 6030 c, t dbSNP:780744512

Target ORF information:

RefSeq Version NM_001242607
Organism Homo sapiens (human)
Definition Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001242607

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGG CTTCGTGGAC TCGACCAGAG
AAGCAAGAGG TACATGCACC ATGGAACTGG CAAGTGGGCA GACAGAAAGG ACAGGCTGGC
AGTGCAGGTT TCCCAGGATC TCATGAGACT CTGGATGGGC ACATGGTGGT GCGTAGCCAT
GCCCGTGTGT CGTCGCTGAC CCTGAAGAGC ATCCAGTACA CTGATGCCGG AGAGTACATC
TGCACCGCCA GCAACACCAT CGGCCAGGAC TCCCAGTCCA TGTACCTTGA AGTGCAATAT
GCCCCAAAGC TACAGGGCCC TGTGGCTGTG TACACTTGGG AGGGGAACCA GGTGAACATC
ACCTGCGAGG TATTTGCCTA TCCCAGTGCC ACGATCTCAT GGTTTCGGGA TGGCCAGCTG
CTGCCAAGCT CCAATTACAG CAATATCAAG ATCTACAACA CCCCCTCTGC CAGCTATCTG
GAGGTGACCC CAGACTCTGA GAATGATTTT GGGAACTACA ACTGTACTGC AGTGAACCGC
ATTGGGCAGG AGTCCTTGGA ATTCATCCTT GTTCAAGCAG ACACCCCCTC TTCACCATCC
ATCGACCAGG TGGAGCCATA CTCCAGCACA GCCCAGGTGC AGTTTGATGA ACCAGAGGCC
ACAGGTGGGG TGCCCATCCT CAAATACAAA GCTGAGTGGA GAGCAGTTGG TGAAGAAGTA
TGGCATTCCA AGTGGTATGA TGCCAAGGAA GCCAGCATGG AGGGCATCGT CACCATCGTG
GGCCTGAAGC CCGAAACAAC GTACGCCGTA AGGCTGGCGG CGCTCAATGG CAAAGGGCTG
GGTGAGATCA GCGCGGCCTC CGAGTTCAAG ACGCAGCCAG TCCAAGGGGA ACCCAGTGCA
CCTAAGCTCG AAGGGCAGAT GGGAGAGGAT GGAAACTCTA TTAAAGTGAA CCTGATCAAG
CAGGATGACG GCGGCTCCCC CATCAGACAC TATCTGGTCA GGTACCGAGC GCTCTCCTCC
GAGTGGAAAC CAGAGATCAG GCTCCCGTCT GGCAGTGACC ACGTCATGCT GAAGTCCCTG
GACTGGAATG CTGAGTATGA GGTCTACGTG GTGGCTGAGA ACCAGCAAGG AAAATCCAAG
GCGGCTCATT TTGTGTTCAG GACCTCGGCC CAGCCCACAG CCATCCCAGC CAACGGCAGC
CCCACCTCAG GCCTGAGCAC CGGGGCCATC GTGGGCATCC TCATCGTCAT CTTCGTCCTG
CTCCTGGTGG TTGTGGACAT CACCTGCTAC TTCCTGAACA AGTGTGGCCT GTTCATGTGC
ATTGCGGTCA ACCTGTGTGG AAAAGCCGGG CCCGGGGCCA AGGGCAAGGA CATGGAGGAG
GGCAAGGCCG CCTTCTCGAA AGATGAGTCC AAGGAGCCCA TCGTGGAGGT TCGAACGGAG
GAGGAGAGGA CCCCAAACCA TGATGGAGGG AAACACACAG AGCCCAACGA GACCACGCCA
CTGACGGAGC CCGAGAAGGG CCCCGTAGAA GCAAAGCCAG AGTGCCAGGA GACAGAAACG
AAGCCAGCGC CAGCCGAAGT CAAGACGGTC CCCAATGACG CCACACAGAC AAAGGAGAAC
GAGAGCAAAG CATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00771
Accession Version NM_001242608.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2181bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neural cell adhesion molecule 1 isoform 4 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from KF511120.1, AB209443.1 and AA029449.1. Summary: This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]. Transcript Variant: This variant (4) lacks 2 alternate in-frame exons and uses an alternate splice site in the 3' coding region compared to variant 5. The resulting protein (isoform 4) is shorter and has a distinct C-terminus compared to isoform 5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209443.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGA CTCTGGATGG GCACATGGTG
GTGCGTAGCC ATGCCCGTGT GTCGTCGCTG ACCCTGAAGA GCATCCAGTA CACTGATGCC
GGAGAGTACA TCTGCACCGC CAGCAACACC ATCGGCCAGG ACTCCCAGTC CATGTACCTT
GAAGTGCAAT ATGCCCCAAA GCTACAGGGC CCTGTGGCTG TGTACACTTG GGAGGGGAAC
CAGGTGAACA TCACCTGCGA GGTATTTGCC TATCCCAGTG CCACGATCTC ATGGTTTCGG
GATGGCCAGC TGCTGCCAAG CTCCAATTAC AGCAATATCA AGATCTACAA CACCCCCTCT
GCCAGCTATC TGGAGGTGAC CCCAGACTCT GAGAATGATT TTGGGAACTA CAACTGTACT
GCAGTGAACC GCATTGGGCA GGAGTCCTTG GAATTCATCC TTGTTCAAGC AGACACCCCC
TCTTCACCAT CCATCGACCA GGTGGAGCCA TACTCCAGCA CAGCCCAGGT GCAGTTTGAT
GAACCAGAGG CCACAGGTGG GGTGCCCATC CTCAAATACA AAGCTGAGTG GAGAGCAGTT
GGTGAAGAAG TATGGCATTC CAAGTGGTAT GATGCCAAGG AAGCCAGCAT GGAGGGCATC
GTCACCATCG TGGGCCTGAA GCCCGAAACA ACGTACGCCG TAAGGCTGGC GGCGCTCAAT
GGCAAAGGGC TGGGTGAGAT CAGCGCGGCC TCCGAGTTCA AGACGCAGCC AGTCCAAGGG
GAACCCAGTG CACCTAAGCT CGAAGGGCAG ATGGGAGAGG ATGGAAACTC TATTAAAGTG
AACCTGATCA AGCAGGATGA CGGCGGCTCC CCCATCAGAC ACTATCTGGT CAGGTACCGA
GCGCTCTCCT CCGAGTGGAA ACCAGAGATC AGGCTCCCGT CTGGCAGTGA CCACGTCATG
CTGAAGTCCC TGGACTGGAA TGCTGAGTAT GAGGTCTACG TGGTGGCTGA GAACCAGCAA
GGAAAATCCA AGGCGGCTCA TTTTGTGTTC AGGACCTCGG CCCAGCCCAC AGCCATCCCA
GCAACCTTGG GAGGCAATTC TGCATCCTAC ACCTTTGTCT CATTGCTTTT CTCTGCAGTG
ACTCTTCTTT TGCTCTGTTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001229537.1
CDS367..2547
Misc Feature(1)424..711(+)
Misc Feature(2)427..702(+)
Misc Feature(3)469..480(+)
Misc Feature(4)757..942(+)
Misc Feature(5)769..936(+)
Misc Feature(6)997..1287(+)
Misc Feature(7)1030..1278(+)
Misc Feature(8)1282..1572(+)
Misc Feature(9)1333..1542(+)
Misc Feature(10)1618..1824(+)
Misc Feature(11)1630..1842(+)
Misc Feature(12)1858..2151(+)
Misc Feature(13)2068..2115(+)
Misc Feature(14)2116..2130(+)
Misc Feature(15)2212..2442(+)
Misc Feature(16)2362..2409(+)
Misc Feature(17)2410..2424(+)
Exon (1)1..418
Gene:NCAM1
Gene Synonym:
Exon (2)419..493
Gene:NCAM1
Gene Synonym:
Exon (3)494..712
Gene:NCAM1
Gene Synonym:
Exon (4)713..856
Gene:NCAM1
Gene Synonym:
Exon (5)857..994
Gene:NCAM1
Gene Synonym:
Exon (6)995..1112
Gene:NCAM1
Gene Synonym:
Exon (7)1113..1282
Gene:NCAM1
Gene Synonym:
Exon (8)1283..1425
Gene:NCAM1
Gene Synonym:
Exon (9)1426..1576
Gene:NCAM1
Gene Synonym:
Exon (10)1577..1761
Gene:NCAM1
Gene Synonym:
Exon (11)1762..1858
Gene:NCAM1
Gene Synonym:
Exon (12)1859..2029
Gene:NCAM1
Gene Synonym:
Exon (13)2030..2161
Gene:NCAM1
Gene Synonym:
Exon (14)2162..2164
Gene:NCAM1
Gene Synonym:
Exon (15)2165..2289
Gene:NCAM1
Gene Synonym:
Exon (16)2290..2467
Gene:NCAM1
Gene Synonym:
Exon (17)2468..4819
Gene:NCAM1
Gene Synonym:
Translation
Position Chain Variation Link
78 78 a, c, g dbSNP:7938749
313 313 a, c, g, t dbSNP:199895300
331 331 c, t dbSNP:201206514
372 372 -, g dbSNP:201772924
373 373 a, c dbSNP:112306738
385 385 a, c, t dbSNP:7105734
390 390 c, g, t dbSNP:6589347
402 402 a, t dbSNP:201161826
1036 1036 a, c dbSNP:199787286
1507 1507 c, g dbSNP:55757519
1510 1510 g, t dbSNP:56107970
1730 1730 a, t dbSNP:200123115
1986 1986 g, t dbSNP:584427
2188 2188 a, g dbSNP:746818916
2340 2340 c, t dbSNP:201880501
2576 2576 g, t dbSNP:2288158
2624 2624 -, atgg dbSNP:146542910
2675 2675 a, g dbSNP:188136769
2823 2823 c, t dbSNP:768824649
3225 3225 g, t dbSNP:762082614
3295 3295 a, g dbSNP:767723581
3505 3505 a, g dbSNP:2298703
3515 3515 a, g dbSNP:7946128
3718 3718 c, t dbSNP:10502171
3906 3906 c, t dbSNP:1006826
4103 4103 c, t dbSNP:72995546
4182 4182 g, t dbSNP:777506545
4183 4183 a, t dbSNP:746254054
4230 4230 c, t dbSNP:625741
4433 4433 a, g dbSNP:111648733
4495 4495 a, g dbSNP:769033510
4679 4679 a, c dbSNP:748160487
4766 4766 c, g dbSNP:654362
4812 4812 c, t dbSNP:766244760

Target ORF information:

RefSeq Version NM_001242608
Organism Homo sapiens (human)
Definition Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001242608

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGA CTCTGGATGG GCACATGGTG
GTGCGTAGCC ATGCCCGTGT GTCGTCGCTG ACCCTGAAGA GCATCCAGTA CACTGATGCC
GGAGAGTACA TCTGCACCGC CAGCAACACC ATCGGCCAGG ACTCCCAGTC CATGTACCTT
GAAGTGCAAT ATGCCCCAAA GCTACAGGGC CCTGTGGCTG TGTACACTTG GGAGGGGAAC
CAGGTGAACA TCACCTGCGA GGTATTTGCC TATCCCAGTG CCACGATCTC ATGGTTTCGG
GATGGCCAGC TGCTGCCAAG CTCCAATTAC AGCAATATCA AGATCTACAA CACCCCCTCT
GCCAGCTATC TGGAGGTGAC CCCAGACTCT GAGAATGATT TTGGGAACTA CAACTGTACT
GCAGTGAACC GCATTGGGCA GGAGTCCTTG GAATTCATCC TTGTTCAAGC AGACACCCCC
TCTTCACCAT CCATCGACCA GGTGGAGCCA TACTCCAGCA CAGCCCAGGT GCAGTTTGAT
GAACCAGAGG CCACAGGTGG GGTGCCCATC CTCAAATACA AAGCTGAGTG GAGAGCAGTT
GGTGAAGAAG TATGGCATTC CAAGTGGTAT GATGCCAAGG AAGCCAGCAT GGAGGGCATC
GTCACCATCG TGGGCCTGAA GCCCGAAACA ACGTACGCCG TAAGGCTGGC GGCGCTCAAT
GGCAAAGGGC TGGGTGAGAT CAGCGCGGCC TCCGAGTTCA AGACGCAGCC AGTCCAAGGG
GAACCCAGTG CACCTAAGCT CGAAGGGCAG ATGGGAGAGG ATGGAAACTC TATTAAAGTG
AACCTGATCA AGCAGGATGA CGGCGGCTCC CCCATCAGAC ACTATCTGGT CAGGTACCGA
GCGCTCTCCT CCGAGTGGAA ACCAGAGATC AGGCTCCCGT CTGGCAGTGA CCACGTCATG
CTGAAGTCCC TGGACTGGAA TGCTGAGTAT GAGGTCTACG TGGTGGCTGA GAACCAGCAA
GGAAAATCCA AGGCGGCTCA TTTTGTGTTC AGGACCTCGG CCCAGCCCAC AGCCATCCCA
GCAACCTTGG GAGGCAATTC TGCATCCTAC ACCTTTGTCT CATTGCTTTT CTCTGCAGTG
ACTCTTCTTT TGCTCTGTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.