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NCF2 neutrophil cytosolic factor 2 [Homo sapiens (human)]

Gene Symbol NCF2
Entrez Gene ID 4688
Full Name neutrophil cytosolic factor 2
Synonyms NCF-2, NOXA2, P67-PHOX, P67PHOX
General protein information
Preferred Names
neutrophil cytosol factor 2
Names
neutrophil cytosol factor 2
NADPH oxidase activator 2
67 kDa neutrophil oxidase factor
neutrophil NADPH oxidase factor 2
chronic granulomatous disease, autosomal 2
neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q25

Summary This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]. lac of sum
Disorder MIM:

608515

Disorder Html: Chronic granulomatous disease due to deficiency of NCF-2, 233710 (3)





The following NCF2 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCF2 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19282 XM_011509580 PREDICTED: Homo sapiens neutrophil cytosolic factor 2 (NCF2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu19282 XM_011509581 PREDICTED: Homo sapiens neutrophil cytosolic factor 2 (NCF2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu34408 XM_005245207 PREDICTED: Homo sapiens neutrophil cytosolic factor 2 (NCF2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu16749 NM_001190789 Homo sapiens neutrophil cytosolic factor 2 (NCF2), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $379
OHu19282 NM_001127651 Homo sapiens neutrophil cytosolic factor 2 (NCF2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $379
OHu23215 NM_001190794 Homo sapiens neutrophil cytosolic factor 2 (NCF2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $409
OHu19282 NM_000433 Homo sapiens neutrophil cytosolic factor 2 (NCF2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $379

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu19282
Accession Version XM_011509580.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1581bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neutrophil cytosol factor 2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCCCTGG TGGAGGCCAT CAGCCTCTGG AATGAAGGGG TGCTGGCAGC GGACAAGAAG 
GACTGGAAGG GAGCCCTGGA TGCCTTCAGT GCCGTCCAGG ACCCCCACTC CCGGATTTGC
TTCAACATTG GCTGCATGTA CACTATCCTG AAGAACATGA CTGAAGCAGA GAAGGCCTTT
ACCAGAAGCA TTAACCGAGA CAAGCACTTG GCAGTGGCTT ACTTCCAACG AGGGATGCTC
TACTACCAGA CAGAGAAATA TGATTTGGCT ATCAAAGACC TTAAAGAAGC CTTGATTCAG
CTTCGAGGGA ACCAGCTGAT AGACTATAAG ATCCTGGGGC TCCAGTTCAA GCTGTTTGCC
TGTGAGGTGT TATATAACAT TGCTTTCATG TATGCCAAGA AGGAGGAATG GAAAAAAGCT
GAAGAACAGT TAGCATTGGC CACGAGCATG AAGTCTGAGC CCAGACATTC CAAAATCGAC
AAGGCGATGG AGTGTGTCTG GAAGCAGAAG CTATATGAGC CAGTGGTGAT CCCTGTGGGC
AAGCTGTTTC GACCAAATGA GAGACAAGTG GCTCAGCTGG CCAAGAAGGA TTACCTAGGC
AAGGCGACGG TCGTGGCATC TGTGGTGGAT CAAGACAGTT TCTCTGGGTT TGCCCCTCTG
CAACCACAGG CAGCTGAGCC TCCACCCAGA CCGAAAACCC CAGAGATCTT CAGGGCTCTG
GAAGGGGAGG CTCACCGTGT GCTATTTGGG TTTGTGCCTG AGACAAAAGA AGAGCTCCAG
GTCATGCCAG GGAACATTGT CTTTGTCTTG AAGAAGGGCA ATGATAACTG GGCCACGGTC
ATGTTCAACG GGCAGAAGGG GCTTGTTCCC TGCAACTACC TTGAACCAGT TGAGCTGCGG
ATCCACCCTC AGCAGCAGCC CCAGGAGGAA AGCTCTCCGC AGTCCGACAT CCCAGCTCCT
CCTAGTTCCA AAGCCCCTGG AAGACCCCAG CTGTCACCAG GCCAGAAACA AAAAGAAGAG
CCTAAGGAAG TGAAGCTCAG TGTTCCCATG CCCTACACAC TCAAGGTGCA CTACAAGTAC
ACGGTAGTCA TGAAGACTCA GCCCGGGCTC CCCTACAGCC AGGTCCGGGA CATGGTGTCT
AAGAAACTGG AGCTCCGGCT GGAACACACT AAGCTGAGCT ATCGGCCTCG GGACAGCAAT
GAGCTGGTGC CCCTTTCAGA AGACAGCATG AAGGATGCCT GGGGCCAGGT GAAAAACTAC
TGCCTGACTC TGTGGTGTGA GAACACAGTG GGTGACCAAG GCTTTCCAGA TGAACCCAAG
GAAAGTGAAA AAGCTGATGC TAATAACCAG ACAACAGAAC CTCAGCTTAA GAAAGGCAGC
CAAGTGGAGG CACTCTTCAG TTATGAGGCT ACCCAACCAG AGGACCTGGA GTTTCAGGAA
GGGGATATAA TCCTGGTGTT ATCAAAGGTG AATGAAGAAT GGCTGGAAGG GGAGTGCAAA
GGGAAGGTGG GCATTTTCCC CAAAGTTTTT GTTGAAGACT GCGCAACTAC AGATTTGGAA
AGCACTCGGA GAGAAGTCTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011507882.1
CDS114..1694
Misc Feature(1)129..209(+)
Misc Feature(2)132..362(+)
Misc Feature(3)219..311(+)
Misc Feature(4)234..407(+)
Misc Feature(5)324..407(+)
Misc Feature(6)324..>407(+)
Misc Feature(7)843..1004(+)
Misc Feature(8)858..992(+)
Misc Feature(9)1164..1400(+)
Misc Feature(10)1176..1367(+)
Misc Feature(11)1494..1652(+)
Misc Feature(12)1509..1643(+)
Translation
Position Chain Variation Link
41 41 a, g dbSNP:764587406
81 81 a, g dbSNP:572906223
84 84 c, t dbSNP:772178602
86 86 a, c dbSNP:748277308
88 88 c, g dbSNP:774655949
97 97 c, t dbSNP:768273338
100 100 g, t dbSNP:749109356
102 102 c, t dbSNP:11540867
108 108 c, g dbSNP:780058052
110 110 a, t dbSNP:35681596
111 111 a, c dbSNP:745844166
114 114 a, c dbSNP:780810631
122 122 a, g dbSNP:756851268
125 125 a, g dbSNP:751114991
136 136 g, t dbSNP:368880633
153 153 g, t dbSNP:763816212
162 162 a, g dbSNP:758057222
163 163 a, c, t dbSNP:201589700
164 164 a, g dbSNP:761136334
174 174 g, t dbSNP:773175637
181 181 a, t dbSNP:767533151
187 187 a, c dbSNP:761962182
188 188 c, t dbSNP:774369490
202 202 a, g dbSNP:544745512
206 206 c, t dbSNP:143889676
207 207 a, g dbSNP:201869337
209 209 c, t dbSNP:769744791
214 214 a, g dbSNP:745752489
216 216 a, c dbSNP:368781381
221 221 c, t dbSNP:770284550
225 225 c, t dbSNP:200824291
226 226 a, g dbSNP:147415774
238 238 a, g dbSNP:137854514
243 243 c, g, t dbSNP:137854510
269 269 a, c, t dbSNP:752384353
288 288 a, c, g dbSNP:545984071
293 293 -, t dbSNP:760439163
306 306 a, g dbSNP:111532709
307 307 a, g dbSNP:778546412
309 309 a, c, t dbSNP:750782115
310 310 a, g, t dbSNP:142803799
314 314 c, t dbSNP:752044043
320 320 c, t dbSNP:764187192
334 334 a, g dbSNP:763140210
342 342 c, t dbSNP:752901695
343 343 a, g dbSNP:119103275
346 346 a, g dbSNP:137854519
348 348 a, g, t dbSNP:137854512
350 350 c, g dbSNP:770674754
356 356 c, t dbSNP:143178891
357 357 a, t dbSNP:201003183
364 364 c, t dbSNP:779088549
377 377 c, t dbSNP:770486258
392 392 c, g dbSNP:137854507
397 397 a, c dbSNP:765461476
403 403 c, g dbSNP:755222977
406 406 c, t dbSNP:754041676
409 409 c, t dbSNP:766146068
411 411 c, g, t dbSNP:119103276
413 413 a, g dbSNP:773131016
417 417 c, t dbSNP:374402066
418 418 a, c, g, t dbSNP:137854515
420 420 a, g dbSNP:768193963
421 421 a, g dbSNP:748898061
435 435 a, g dbSNP:749367579
436 436 a, t dbSNP:137854509
437 437 c, t dbSNP:775020616
445 445 c, t dbSNP:771262057
451 451 a, g dbSNP:200286542
455 455 c, g dbSNP:778259155
461 461 c, t dbSNP:758956466
470 470 c, t dbSNP:748707281
486 486 c, t dbSNP:773422870
487 487 a, g dbSNP:772598020
491 491 c, t dbSNP:748619343
496 496 c, t dbSNP:119103274
503 503 g, t dbSNP:779573048
509 509 c, g dbSNP:768786785
519 519 g, t dbSNP:749319711
522 522 a, t dbSNP:137854516
530 530 a, t dbSNP:780235098
532 532 a, c dbSNP:137854520
539 539 a, g dbSNP:779087160
549 549 c, t dbSNP:750697066
556 556 a, c, t dbSNP:764142541
557 557 a, g dbSNP:757064950
566 566 a, g dbSNP:751432737
570 570 a, g dbSNP:533500800
590 590 a, c, t dbSNP:369845453
592 592 a, t dbSNP:267606912
594 594 a, g dbSNP:137878529
597 597 a, g dbSNP:752127520
598 598 c, t dbSNP:376994104
599 599 a, g dbSNP:759074773
601 601 c, t dbSNP:372773130
605 605 a, g dbSNP:772503928
606 606 a, t dbSNP:762270172
609 609 c, g dbSNP:774918809
611 611 c, g dbSNP:769269175
618 618 c, g dbSNP:137854517
621 621 a, g dbSNP:769704327
624 624 c, t dbSNP:145354685
626 626 a, g, t dbSNP:771120104
633 633 c, t dbSNP:747272727
638 638 a, g dbSNP:777507234
641 641 a, g dbSNP:758239702
655 655 a, g dbSNP:2274064
659 659 a, g dbSNP:778911214
663 663 c, t dbSNP:766745748
664 664 c, g dbSNP:137854518
676 676 a, g dbSNP:115365142
678 678 a, c dbSNP:755796920
679 679 a, g dbSNP:750131635
687 687 c, g dbSNP:764314608
699 699 -, aag dbSNP:773850705
701 701 g, t dbSNP:554848021
708 708 a, c dbSNP:763388019
718 718 c, t dbSNP:137854508
719 719 a, g dbSNP:17849501
720 720 a, g dbSNP:765762727
721 721 c, t dbSNP:760085363
722 722 a, g dbSNP:147657171
725 725 c, t dbSNP:772322729
726 726 a, g dbSNP:761985515
730 730 c, t dbSNP:774160536
742 742 a, t dbSNP:768602664
743 743 a, t dbSNP:749187972
746 746 a, g dbSNP:780154260
749 749 c, t dbSNP:774027102
758 758 c, t dbSNP:745372047
774 774 c, t dbSNP:140028944
785 785 a, t dbSNP:758117639
786 786 c, g dbSNP:754192116
795 795 c, t dbSNP:780442146
803 803 a, c dbSNP:756610730
804 804 c, g dbSNP:750922183
805 805 c, t dbSNP:144451516
815 815 a, g dbSNP:757295495
816 816 a, g dbSNP:150595188
820 820 c, t dbSNP:764405660
829 829 c, g dbSNP:753556861
831 831 c, t dbSNP:766232480
837 837 a, g dbSNP:760561718
846 846 c, t dbSNP:750380443
848 848 c, t dbSNP:766952838
849 849 c, t dbSNP:761315970
850 850 a, g, t dbSNP:768181039
855 855 c, t dbSNP:762714261
872 872 c, t dbSNP:776822572
890 890 c, t dbSNP:771349846
897 897 a, t dbSNP:747462402
908 908 c, t dbSNP:778431195
910 910 c, t dbSNP:772775720
918 918 a, g dbSNP:748228594
920 920 c, t dbSNP:371493437
927 927 -, aag dbSNP:778751639
929 929 a, g dbSNP:557535861
933 933 a, g dbSNP:539632630
934 934 a, g dbSNP:754118806
949 949 a, c, t dbSNP:13306581
953 953 c, g dbSNP:554005946
962 962 c, t dbSNP:767437169
963 963 g, t dbSNP:761796267
989 989 c, g dbSNP:757931282
995 995 c, t dbSNP:752217634
1000 1000 a, c, g dbSNP:549428558
1003 1003 c, t dbSNP:35937854
1008 1008 c, t dbSNP:1126596
1011 1011 c, t dbSNP:768029053
1012 1012 a, g dbSNP:762173491
1015 1015 c, t dbSNP:771296779
1017 1017 -, c dbSNP:777621636
1023 1023 c, g dbSNP:774932046
1028 1028 c, g dbSNP:768549092
1031 1031 a, g dbSNP:535561432
1036 1036 a, g dbSNP:775489066
1040 1040 a, g dbSNP:112336681
1051 1051 c, t dbSNP:137937390
1052 1052 a, c, g dbSNP:556389548
1056 1056 c, t dbSNP:556255666
1058 1058 c, t dbSNP:200655126
1059 1059 a, g dbSNP:778521448
1061 1061 c, t dbSNP:754545169
1064 1064 c, t dbSNP:753471440
1071 1071 c, g, t dbSNP:755778188
1074 1074 a, c, t dbSNP:751928437
1077 1077 a, g dbSNP:764404690
1090 1090 c, t dbSNP:369515038
1092 1092 a, g dbSNP:138860204
1096 1096 a, g dbSNP:137854511
1098 1098 a, c dbSNP:765235671
1104 1104 c, t dbSNP:199658789
1106 1106 a, g dbSNP:776750922
1130 1130 -, aga dbSNP:757305871
1131 1131 a, g dbSNP:755689355
1158 1158 c, t dbSNP:745458881
1164 1164 c, t dbSNP:780702980
1165 1165 c, t dbSNP:571460195
1168 1168 a, g dbSNP:748489095
1182 1182 c, t dbSNP:765400770
1186 1186 a, g dbSNP:755434573
1187 1187 c, t dbSNP:546373985
1194 1194 a, t dbSNP:147744729
1196 1196 a, g dbSNP:756157300
1200 1200 a, g dbSNP:560823166
1216 1216 a, c dbSNP:767622954
1217 1217 c, g, t dbSNP:541838904
1218 1218 a, g dbSNP:137854513
1220 1220 a, g dbSNP:762936258
1223 1223 c, t dbSNP:775303126
1235 1235 c, g dbSNP:769837205
1239 1239 c, t dbSNP:777251055
1240 1240 a, g dbSNP:369205421
1246 1246 a, t dbSNP:776252831
1263 1263 a, g dbSNP:770537584
1269 1269 c, t dbSNP:746692621
1270 1270 a, g dbSNP:147908264
1278 1278 -, cacacta dbSNP:756393096
1278 1278 a, c dbSNP:201325416
1280 1280 a, c, t dbSNP:17849502
1291 1291 a, g dbSNP:756705355
1293 1293 g, t dbSNP:749606885
1295 1295 c, t dbSNP:538760999
1296 1296 a, c, g, t dbSNP:13306575
1297 1297 a, g dbSNP:145229115
1300 1300 a, c dbSNP:371503781
1302 1302 c, t dbSNP:145970396
1303 1303 a, g dbSNP:370251291
1304 1304 a, g dbSNP:758694739
1313 1313 c, t dbSNP:148005568
1314 1314 a, g dbSNP:765110090
1325 1325 c, t dbSNP:528239550
1332 1332 a, g dbSNP:753825493
1336 1336 a, g dbSNP:765806399
1338 1338 -, a dbSNP:759398660
1349 1349 g, t dbSNP:760169471
1355 1355 g, t dbSNP:377591562
1357 1357 -, g dbSNP:751478804
1369 1369 a, t dbSNP:35012521
1383 1383 -, ct dbSNP:765868844
1386 1386 c, t dbSNP:373876135
1393 1393 a, g dbSNP:775679393
1397 1397 c, g, t dbSNP:745334269
1407 1407 c, g dbSNP:774068321
1413 1413 c, g dbSNP:765454120
1429 1429 c, g dbSNP:534011480
1434 1434 a, g dbSNP:759940920
1436 1436 a, g dbSNP:777183751
1452 1452 a, g dbSNP:771532464
1453 1453 c, t dbSNP:747548282
1455 1455 a, g dbSNP:141569987
1472 1472 a, c dbSNP:772204296
1473 1473 c, t dbSNP:55761650
1477 1477 a, g dbSNP:142633260
1479 1479 a, c dbSNP:779188117
1486 1486 a, c dbSNP:755209791
1490 1490 c, t dbSNP:749065525
1514 1514 c, t dbSNP:779867537
1518 1518 a, g dbSNP:756008519
1523 1523 c, t dbSNP:750384875
1532 1532 a, g dbSNP:766880844
1538 1538 a, c dbSNP:756797744
1557 1557 a, g dbSNP:751096649
1559 1559 c, t dbSNP:146762552
1565 1565 c, t dbSNP:139817332
1570 1570 c, t dbSNP:762733492
1603 1603 a, g dbSNP:749546475
1604 1604 c, g dbSNP:556748175
1609 1609 a, g dbSNP:755918616
1614 1614 a, g dbSNP:745740745
1616 1616 a, g dbSNP:544453576
1628 1628 c, t dbSNP:757201201
1637 1637 -, a dbSNP:772569356
1644 1644 a, g dbSNP:376920441
1654 1654 a, g dbSNP:183100144
1655 1655 c, t dbSNP:769779675
1656 1656 a, g dbSNP:369006606
1665 1665 c, g dbSNP:143901397
1667 1667 g, t dbSNP:534490818
1675 1675 a, g dbSNP:374667798
1681 1681 a, g, t dbSNP:139108402
1686 1686 -, ga dbSNP:746336607
1689 1689 c, g dbSNP:762269733
1691 1691 c, t dbSNP:781431052
1694 1694 a, g dbSNP:768748386
1696 1696 a, c, t dbSNP:200240164
1701 1701 c, t dbSNP:370795833
1708 1708 c, g dbSNP:770071182
1716 1716 c, g dbSNP:745650507
1721 1721 a, g dbSNP:560754812
1724 1724 a, g dbSNP:780899104
1743 1743 c, g, t dbSNP:746942426
1747 1747 -, ttgt dbSNP:778645987
1752 1752 c, t dbSNP:536373941
1756 1756 -, taagatttag dbSNP:777040106
1759 1759 a, c dbSNP:770972754
1772 1772 c, t dbSNP:568830928
1778 1778 a, g dbSNP:187489898
1791 1791 a, c dbSNP:367946786
1810 1810 c, t dbSNP:532135800
1860 1860 g, t dbSNP:747039614
1910 1910 a, g dbSNP:571212408
1922 1922 c, g dbSNP:182819269
1941 1941 c, t dbSNP:777856745
1956 1956 c, t dbSNP:201050705
1968 1968 -, ttc dbSNP:34035622
1985 1985 -, gtt dbSNP:775582279
2014 2014 a, g dbSNP:528361273
2015 2015 -, acta dbSNP:776233541
2016 2016 -, cattca dbSNP:768663267
2020 2020 -, t dbSNP:746883471
2029 2029 -, a dbSNP:779658024
2052 2052 g, t dbSNP:578107077
2066 2066 -, gt dbSNP:202202602
2087 2087 -, a dbSNP:771465757
2092 2092 -, cat dbSNP:397951870
2092 2092 g, t dbSNP:796860
2092 2092 g, t, tcat dbSNP:369000587
2095 2095 -, cat dbSNP:61684650
2096 2096 -, ac, atc dbSNP:13306577
2097 2097 -, catct dbSNP:543935249
2139 2139 -, agta dbSNP:767143237
2152 2152 c, t dbSNP:758760667
2161 2161 c, t dbSNP:375774362
2174 2174 c, g dbSNP:563118156
2188 2188 g, t dbSNP:200423742
2206 2206 -, ggtta dbSNP:555649211
2211 2211 -, t dbSNP:573207861
2226 2226 g, t dbSNP:753309403
2236 2236 -, gttt dbSNP:757261466
2237 2237 -, gttt dbSNP:376681445
2240 2240 -, gttt dbSNP:767119882

Target ORF information:

RefSeq Version XM_011509580
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neutrophil cytosolic factor 2 (NCF2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509580

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCCCTGG TGGAGGCCAT CAGCCTCTGG AATGAAGGGG TGCTGGCAGC GGACAAGAAG 
GACTGGAAGG GAGCCCTGGA TGCCTTCAGT GCCGTCCAGG ACCCCCACTC CCGGATTTGC
TTCAACATTG GCTGCATGTA CACTATCCTG AAGAACATGA CTGAAGCAGA GAAGGCCTTT
ACCAGAAGCA TTAACCGAGA CAAGCACTTG GCAGTGGCTT ACTTCCAACG AGGGATGCTC
TACTACCAGA CAGAGAAATA TGATTTGGCT ATCAAAGACC TTAAAGAAGC CTTGATTCAG
CTTCGAGGGA ACCAGCTGAT AGACTATAAG ATCCTGGGGC TCCAGTTCAA GCTGTTTGCC
TGTGAGGTGT TATATAACAT TGCTTTCATG TATGCCAAGA AGGAGGAATG GAAAAAAGCT
GAAGAACAGT TAGCATTGGC CACGAGCATG AAGTCTGAGC CCAGACATTC CAAAATCGAC
AAGGCGATGG AGTGTGTCTG GAAGCAGAAG CTATATGAGC CAGTGGTGAT CCCTGTGGGC
AAGCTGTTTC GACCAAATGA GAGACAAGTG GCTCAGCTGG CCAAGAAGGA TTACCTAGGC
AAGGCGACGG TCGTGGCATC TGTGGTGGAT CAAGACAGTT TCTCTGGGTT TGCCCCTCTG
CAACCACAGG CAGCTGAGCC TCCACCCAGA CCGAAAACCC CAGAGATCTT CAGGGCTCTG
GAAGGGGAGG CTCACCGTGT GCTATTTGGG TTTGTGCCTG AGACAAAAGA AGAGCTCCAG
GTCATGCCAG GGAACATTGT CTTTGTCTTG AAGAAGGGCA ATGATAACTG GGCCACGGTC
ATGTTCAACG GGCAGAAGGG GCTTGTTCCC TGCAACTACC TTGAACCAGT TGAGCTGCGG
ATCCACCCTC AGCAGCAGCC CCAGGAGGAA AGCTCTCCGC AGTCCGACAT CCCAGCTCCT
CCTAGTTCCA AAGCCCCTGG AAGACCCCAG CTGTCACCAG GCCAGAAACA AAAAGAAGAG
CCTAAGGAAG TGAAGCTCAG TGTTCCCATG CCCTACACAC TCAAGGTGCA CTACAAGTAC
ACGGTAGTCA TGAAGACTCA GCCCGGGCTC CCCTACAGCC AGGTCCGGGA CATGGTGTCT
AAGAAACTGG AGCTCCGGCT GGAACACACT AAGCTGAGCT ATCGGCCTCG GGACAGCAAT
GAGCTGGTGC CCCTTTCAGA AGACAGCATG AAGGATGCCT GGGGCCAGGT GAAAAACTAC
TGCCTGACTC TGTGGTGTGA GAACACAGTG GGTGACCAAG GCTTTCCAGA TGAACCCAAG
GAAAGTGAAA AAGCTGATGC TAATAACCAG ACAACAGAAC CTCAGCTTAA GAAAGGCAGC
CAAGTGGAGG CACTCTTCAG TTATGAGGCT ACCCAACCAG AGGACCTGGA GTTTCAGGAA
GGGGATATAA TCCTGGTGTT ATCAAAGGTG AATGAAGAAT GGCTGGAAGG GGAGTGCAAA
GGGAAGGTGG GCATTTTCCC CAAAGTTTTT GTTGAAGACT GCGCAACTAC AGATTTGGAA
AGCACTCGGA GAGAAGTCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19282
Accession Version XM_011509581.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1581bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neutrophil cytosol factor 2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCCCTGG TGGAGGCCAT CAGCCTCTGG AATGAAGGGG TGCTGGCAGC GGACAAGAAG 
GACTGGAAGG GAGCCCTGGA TGCCTTCAGT GCCGTCCAGG ACCCCCACTC CCGGATTTGC
TTCAACATTG GCTGCATGTA CACTATCCTG AAGAACATGA CTGAAGCAGA GAAGGCCTTT
ACCAGAAGCA TTAACCGAGA CAAGCACTTG GCAGTGGCTT ACTTCCAACG AGGGATGCTC
TACTACCAGA CAGAGAAATA TGATTTGGCT ATCAAAGACC TTAAAGAAGC CTTGATTCAG
CTTCGAGGGA ACCAGCTGAT AGACTATAAG ATCCTGGGGC TCCAGTTCAA GCTGTTTGCC
TGTGAGGTGT TATATAACAT TGCTTTCATG TATGCCAAGA AGGAGGAATG GAAAAAAGCT
GAAGAACAGT TAGCATTGGC CACGAGCATG AAGTCTGAGC CCAGACATTC CAAAATCGAC
AAGGCGATGG AGTGTGTCTG GAAGCAGAAG CTATATGAGC CAGTGGTGAT CCCTGTGGGC
AAGCTGTTTC GACCAAATGA GAGACAAGTG GCTCAGCTGG CCAAGAAGGA TTACCTAGGC
AAGGCGACGG TCGTGGCATC TGTGGTGGAT CAAGACAGTT TCTCTGGGTT TGCCCCTCTG
CAACCACAGG CAGCTGAGCC TCCACCCAGA CCGAAAACCC CAGAGATCTT CAGGGCTCTG
GAAGGGGAGG CTCACCGTGT GCTATTTGGG TTTGTGCCTG AGACAAAAGA AGAGCTCCAG
GTCATGCCAG GGAACATTGT CTTTGTCTTG AAGAAGGGCA ATGATAACTG GGCCACGGTC
ATGTTCAACG GGCAGAAGGG GCTTGTTCCC TGCAACTACC TTGAACCAGT TGAGCTGCGG
ATCCACCCTC AGCAGCAGCC CCAGGAGGAA AGCTCTCCGC AGTCCGACAT CCCAGCTCCT
CCTAGTTCCA AAGCCCCTGG AAGACCCCAG CTGTCACCAG GCCAGAAACA AAAAGAAGAG
CCTAAGGAAG TGAAGCTCAG TGTTCCCATG CCCTACACAC TCAAGGTGCA CTACAAGTAC
ACGGTAGTCA TGAAGACTCA GCCCGGGCTC CCCTACAGCC AGGTCCGGGA CATGGTGTCT
AAGAAACTGG AGCTCCGGCT GGAACACACT AAGCTGAGCT ATCGGCCTCG GGACAGCAAT
GAGCTGGTGC CCCTTTCAGA AGACAGCATG AAGGATGCCT GGGGCCAGGT GAAAAACTAC
TGCCTGACTC TGTGGTGTGA GAACACAGTG GGTGACCAAG GCTTTCCAGA TGAACCCAAG
GAAAGTGAAA AAGCTGATGC TAATAACCAG ACAACAGAAC CTCAGCTTAA GAAAGGCAGC
CAAGTGGAGG CACTCTTCAG TTATGAGGCT ACCCAACCAG AGGACCTGGA GTTTCAGGAA
GGGGATATAA TCCTGGTGTT ATCAAAGGTG AATGAAGAAT GGCTGGAAGG GGAGTGCAAA
GGGAAGGTGG GCATTTTCCC CAAAGTTTTT GTTGAAGACT GCGCAACTAC AGATTTGGAA
AGCACTCGGA GAGAAGTCTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011507883.1
CDS101..1681
Misc Feature(1)116..196(+)
Misc Feature(2)119..349(+)
Misc Feature(3)206..298(+)
Misc Feature(4)221..394(+)
Misc Feature(5)311..394(+)
Misc Feature(6)311..>394(+)
Misc Feature(7)830..991(+)
Misc Feature(8)845..979(+)
Misc Feature(9)1151..1387(+)
Misc Feature(10)1163..1354(+)
Misc Feature(11)1481..1639(+)
Misc Feature(12)1496..1630(+)
Translation
Position Chain Variation Link
5 5 a, g dbSNP:574011269
14 14 -, a dbSNP:35066000
16 16 -, ctct dbSNP:771477568
16 16 -, ct dbSNP:768809741
23 23 g, t dbSNP:2274065
24 24 -, ctt dbSNP:199728796
36 36 c, t dbSNP:569365439
71 71 c, t dbSNP:772178602
73 73 a, c dbSNP:748277308
75 75 c, g dbSNP:774655949
84 84 c, t dbSNP:768273338
87 87 g, t dbSNP:749109356
89 89 c, t dbSNP:11540867
95 95 c, g dbSNP:780058052
97 97 a, t dbSNP:35681596
98 98 a, c dbSNP:745844166
101 101 a, c dbSNP:780810631
109 109 a, g dbSNP:756851268
112 112 a, g dbSNP:751114991
123 123 g, t dbSNP:368880633
140 140 g, t dbSNP:763816212
149 149 a, g dbSNP:758057222
150 150 a, c, t dbSNP:201589700
151 151 a, g dbSNP:761136334
161 161 g, t dbSNP:773175637
168 168 a, t dbSNP:767533151
174 174 a, c dbSNP:761962182
175 175 c, t dbSNP:774369490
189 189 a, g dbSNP:544745512
193 193 c, t dbSNP:143889676
194 194 a, g dbSNP:201869337
196 196 c, t dbSNP:769744791
201 201 a, g dbSNP:745752489
203 203 a, c dbSNP:368781381
208 208 c, t dbSNP:770284550
212 212 c, t dbSNP:200824291
213 213 a, g dbSNP:147415774
225 225 a, g dbSNP:137854514
230 230 c, g, t dbSNP:137854510
256 256 a, c, t dbSNP:752384353
275 275 a, c, g dbSNP:545984071
280 280 -, t dbSNP:760439163
293 293 a, g dbSNP:111532709
294 294 a, g dbSNP:778546412
296 296 a, c, t dbSNP:750782115
297 297 a, g, t dbSNP:142803799
301 301 c, t dbSNP:752044043
307 307 c, t dbSNP:764187192
321 321 a, g dbSNP:763140210
329 329 c, t dbSNP:752901695
330 330 a, g dbSNP:119103275
333 333 a, g dbSNP:137854519
335 335 a, g, t dbSNP:137854512
337 337 c, g dbSNP:770674754
343 343 c, t dbSNP:143178891
344 344 a, t dbSNP:201003183
351 351 c, t dbSNP:779088549
364 364 c, t dbSNP:770486258
379 379 c, g dbSNP:137854507
384 384 a, c dbSNP:765461476
390 390 c, g dbSNP:755222977
393 393 c, t dbSNP:754041676
396 396 c, t dbSNP:766146068
398 398 c, g, t dbSNP:119103276
400 400 a, g dbSNP:773131016
404 404 c, t dbSNP:374402066
405 405 a, c, g, t dbSNP:137854515
407 407 a, g dbSNP:768193963
408 408 a, g dbSNP:748898061
422 422 a, g dbSNP:749367579
423 423 a, t dbSNP:137854509
424 424 c, t dbSNP:775020616
432 432 c, t dbSNP:771262057
438 438 a, g dbSNP:200286542
442 442 c, g dbSNP:778259155
448 448 c, t dbSNP:758956466
457 457 c, t dbSNP:748707281
473 473 c, t dbSNP:773422870
474 474 a, g dbSNP:772598020
478 478 c, t dbSNP:748619343
483 483 c, t dbSNP:119103274
490 490 g, t dbSNP:779573048
496 496 c, g dbSNP:768786785
506 506 g, t dbSNP:749319711
509 509 a, t dbSNP:137854516
517 517 a, t dbSNP:780235098
519 519 a, c dbSNP:137854520
526 526 a, g dbSNP:779087160
536 536 c, t dbSNP:750697066
543 543 a, c, t dbSNP:764142541
544 544 a, g dbSNP:757064950
553 553 a, g dbSNP:751432737
557 557 a, g dbSNP:533500800
577 577 a, c, t dbSNP:369845453
579 579 a, t dbSNP:267606912
581 581 a, g dbSNP:137878529
584 584 a, g dbSNP:752127520
585 585 c, t dbSNP:376994104
586 586 a, g dbSNP:759074773
588 588 c, t dbSNP:372773130
592 592 a, g dbSNP:772503928
593 593 a, t dbSNP:762270172
596 596 c, g dbSNP:774918809
598 598 c, g dbSNP:769269175
605 605 c, g dbSNP:137854517
608 608 a, g dbSNP:769704327
611 611 c, t dbSNP:145354685
613 613 a, g, t dbSNP:771120104
620 620 c, t dbSNP:747272727
625 625 a, g dbSNP:777507234
628 628 a, g dbSNP:758239702
642 642 a, g dbSNP:2274064
646 646 a, g dbSNP:778911214
650 650 c, t dbSNP:766745748
651 651 c, g dbSNP:137854518
663 663 a, g dbSNP:115365142
665 665 a, c dbSNP:755796920
666 666 a, g dbSNP:750131635
674 674 c, g dbSNP:764314608
686 686 -, aag dbSNP:773850705
688 688 g, t dbSNP:554848021
695 695 a, c dbSNP:763388019
705 705 c, t dbSNP:137854508
706 706 a, g dbSNP:17849501
707 707 a, g dbSNP:765762727
708 708 c, t dbSNP:760085363
709 709 a, g dbSNP:147657171
712 712 c, t dbSNP:772322729
713 713 a, g dbSNP:761985515
717 717 c, t dbSNP:774160536
729 729 a, t dbSNP:768602664
730 730 a, t dbSNP:749187972
733 733 a, g dbSNP:780154260
736 736 c, t dbSNP:774027102
745 745 c, t dbSNP:745372047
761 761 c, t dbSNP:140028944
772 772 a, t dbSNP:758117639
773 773 c, g dbSNP:754192116
782 782 c, t dbSNP:780442146
790 790 a, c dbSNP:756610730
791 791 c, g dbSNP:750922183
792 792 c, t dbSNP:144451516
802 802 a, g dbSNP:757295495
803 803 a, g dbSNP:150595188
807 807 c, t dbSNP:764405660
816 816 c, g dbSNP:753556861
818 818 c, t dbSNP:766232480
824 824 a, g dbSNP:760561718
833 833 c, t dbSNP:750380443
835 835 c, t dbSNP:766952838
836 836 c, t dbSNP:761315970
837 837 a, g, t dbSNP:768181039
842 842 c, t dbSNP:762714261
859 859 c, t dbSNP:776822572
877 877 c, t dbSNP:771349846
884 884 a, t dbSNP:747462402
895 895 c, t dbSNP:778431195
897 897 c, t dbSNP:772775720
905 905 a, g dbSNP:748228594
907 907 c, t dbSNP:371493437
914 914 -, aag dbSNP:778751639
916 916 a, g dbSNP:557535861
920 920 a, g dbSNP:539632630
921 921 a, g dbSNP:754118806
936 936 a, c, t dbSNP:13306581
940 940 c, g dbSNP:554005946
949 949 c, t dbSNP:767437169
950 950 g, t dbSNP:761796267
976 976 c, g dbSNP:757931282
982 982 c, t dbSNP:752217634
987 987 a, c, g dbSNP:549428558
990 990 c, t dbSNP:35937854
995 995 c, t dbSNP:1126596
998 998 c, t dbSNP:768029053
999 999 a, g dbSNP:762173491
1002 1002 c, t dbSNP:771296779
1004 1004 -, c dbSNP:777621636
1010 1010 c, g dbSNP:774932046
1015 1015 c, g dbSNP:768549092
1018 1018 a, g dbSNP:535561432
1023 1023 a, g dbSNP:775489066
1027 1027 a, g dbSNP:112336681
1038 1038 c, t dbSNP:137937390
1039 1039 a, c, g dbSNP:556389548
1043 1043 c, t dbSNP:556255666
1045 1045 c, t dbSNP:200655126
1046 1046 a, g dbSNP:778521448
1048 1048 c, t dbSNP:754545169
1051 1051 c, t dbSNP:753471440
1058 1058 c, g, t dbSNP:755778188
1061 1061 a, c, t dbSNP:751928437
1064 1064 a, g dbSNP:764404690
1077 1077 c, t dbSNP:369515038
1079 1079 a, g dbSNP:138860204
1083 1083 a, g dbSNP:137854511
1085 1085 a, c dbSNP:765235671
1091 1091 c, t dbSNP:199658789
1093 1093 a, g dbSNP:776750922
1117 1117 -, aga dbSNP:757305871
1118 1118 a, g dbSNP:755689355
1145 1145 c, t dbSNP:745458881
1151 1151 c, t dbSNP:780702980
1152 1152 c, t dbSNP:571460195
1155 1155 a, g dbSNP:748489095
1169 1169 c, t dbSNP:765400770
1173 1173 a, g dbSNP:755434573
1174 1174 c, t dbSNP:546373985
1181 1181 a, t dbSNP:147744729
1183 1183 a, g dbSNP:756157300
1187 1187 a, g dbSNP:560823166
1203 1203 a, c dbSNP:767622954
1204 1204 c, g, t dbSNP:541838904
1205 1205 a, g dbSNP:137854513
1207 1207 a, g dbSNP:762936258
1210 1210 c, t dbSNP:775303126
1222 1222 c, g dbSNP:769837205
1226 1226 c, t dbSNP:777251055
1227 1227 a, g dbSNP:369205421
1233 1233 a, t dbSNP:776252831
1250 1250 a, g dbSNP:770537584
1256 1256 c, t dbSNP:746692621
1257 1257 a, g dbSNP:147908264
1265 1265 -, cacacta dbSNP:756393096
1265 1265 a, c dbSNP:201325416
1267 1267 a, c, t dbSNP:17849502
1278 1278 a, g dbSNP:756705355
1280 1280 g, t dbSNP:749606885
1282 1282 c, t dbSNP:538760999
1283 1283 a, c, g, t dbSNP:13306575
1284 1284 a, g dbSNP:145229115
1287 1287 a, c dbSNP:371503781
1289 1289 c, t dbSNP:145970396
1290 1290 a, g dbSNP:370251291
1291 1291 a, g dbSNP:758694739
1300 1300 c, t dbSNP:148005568
1301 1301 a, g dbSNP:765110090
1312 1312 c, t dbSNP:528239550
1319 1319 a, g dbSNP:753825493
1323 1323 a, g dbSNP:765806399
1325 1325 -, a dbSNP:759398660
1336 1336 g, t dbSNP:760169471
1342 1342 g, t dbSNP:377591562
1344 1344 -, g dbSNP:751478804
1356 1356 a, t dbSNP:35012521
1370 1370 -, ct dbSNP:765868844
1373 1373 c, t dbSNP:373876135
1380 1380 a, g dbSNP:775679393
1384 1384 c, g, t dbSNP:745334269
1394 1394 c, g dbSNP:774068321
1400 1400 c, g dbSNP:765454120
1416 1416 c, g dbSNP:534011480
1421 1421 a, g dbSNP:759940920
1423 1423 a, g dbSNP:777183751
1439 1439 a, g dbSNP:771532464
1440 1440 c, t dbSNP:747548282
1442 1442 a, g dbSNP:141569987
1459 1459 a, c dbSNP:772204296
1460 1460 c, t dbSNP:55761650
1464 1464 a, g dbSNP:142633260
1466 1466 a, c dbSNP:779188117
1473 1473 a, c dbSNP:755209791
1477 1477 c, t dbSNP:749065525
1501 1501 c, t dbSNP:779867537
1505 1505 a, g dbSNP:756008519
1510 1510 c, t dbSNP:750384875
1519 1519 a, g dbSNP:766880844
1525 1525 a, c dbSNP:756797744
1544 1544 a, g dbSNP:751096649
1546 1546 c, t dbSNP:146762552
1552 1552 c, t dbSNP:139817332
1557 1557 c, t dbSNP:762733492
1590 1590 a, g dbSNP:749546475
1591 1591 c, g dbSNP:556748175
1596 1596 a, g dbSNP:755918616
1601 1601 a, g dbSNP:745740745
1603 1603 a, g dbSNP:544453576
1615 1615 c, t dbSNP:757201201
1624 1624 -, a dbSNP:772569356
1631 1631 a, g dbSNP:376920441
1641 1641 a, g dbSNP:183100144
1642 1642 c, t dbSNP:769779675
1643 1643 a, g dbSNP:369006606
1652 1652 c, g dbSNP:143901397
1654 1654 g, t dbSNP:534490818
1662 1662 a, g dbSNP:374667798
1668 1668 a, g, t dbSNP:139108402
1673 1673 -, ga dbSNP:746336607
1676 1676 c, g dbSNP:762269733
1678 1678 c, t dbSNP:781431052
1681 1681 a, g dbSNP:768748386
1683 1683 a, c, t dbSNP:200240164
1688 1688 c, t dbSNP:370795833
1695 1695 c, g dbSNP:770071182
1703 1703 c, g dbSNP:745650507
1708 1708 a, g dbSNP:560754812
1711 1711 a, g dbSNP:780899104
1730 1730 c, g, t dbSNP:746942426
1734 1734 -, ttgt dbSNP:778645987
1739 1739 c, t dbSNP:536373941
1743 1743 -, taagatttag dbSNP:777040106
1746 1746 a, c dbSNP:770972754
1759 1759 c, t dbSNP:568830928
1765 1765 a, g dbSNP:187489898
1778 1778 a, c dbSNP:367946786
1797 1797 c, t dbSNP:532135800
1847 1847 g, t dbSNP:747039614
1897 1897 a, g dbSNP:571212408
1909 1909 c, g dbSNP:182819269
1928 1928 c, t dbSNP:777856745
1943 1943 c, t dbSNP:201050705
1955 1955 -, ttc dbSNP:34035622
1972 1972 -, gtt dbSNP:775582279
2001 2001 a, g dbSNP:528361273
2002 2002 -, acta dbSNP:776233541
2003 2003 -, cattca dbSNP:768663267
2007 2007 -, t dbSNP:746883471
2016 2016 -, a dbSNP:779658024
2039 2039 g, t dbSNP:578107077
2053 2053 -, gt dbSNP:202202602
2074 2074 -, a dbSNP:771465757
2079 2079 -, cat dbSNP:397951870
2079 2079 g, t dbSNP:796860
2079 2079 g, t, tcat dbSNP:369000587
2082 2082 -, cat dbSNP:61684650
2083 2083 -, ac, atc dbSNP:13306577
2084 2084 -, catct dbSNP:543935249
2126 2126 -, agta dbSNP:767143237
2139 2139 c, t dbSNP:758760667
2148 2148 c, t dbSNP:375774362
2161 2161 c, g dbSNP:563118156
2175 2175 g, t dbSNP:200423742
2193 2193 -, ggtta dbSNP:555649211
2198 2198 -, t dbSNP:573207861
2213 2213 g, t dbSNP:753309403
2223 2223 -, gttt dbSNP:757261466
2224 2224 -, gttt dbSNP:376681445
2227 2227 -, gttt dbSNP:767119882

Target ORF information:

RefSeq Version XM_011509581
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neutrophil cytosolic factor 2 (NCF2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509581

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCCCTGG TGGAGGCCAT CAGCCTCTGG AATGAAGGGG TGCTGGCAGC GGACAAGAAG 
GACTGGAAGG GAGCCCTGGA TGCCTTCAGT GCCGTCCAGG ACCCCCACTC CCGGATTTGC
TTCAACATTG GCTGCATGTA CACTATCCTG AAGAACATGA CTGAAGCAGA GAAGGCCTTT
ACCAGAAGCA TTAACCGAGA CAAGCACTTG GCAGTGGCTT ACTTCCAACG AGGGATGCTC
TACTACCAGA CAGAGAAATA TGATTTGGCT ATCAAAGACC TTAAAGAAGC CTTGATTCAG
CTTCGAGGGA ACCAGCTGAT AGACTATAAG ATCCTGGGGC TCCAGTTCAA GCTGTTTGCC
TGTGAGGTGT TATATAACAT TGCTTTCATG TATGCCAAGA AGGAGGAATG GAAAAAAGCT
GAAGAACAGT TAGCATTGGC CACGAGCATG AAGTCTGAGC CCAGACATTC CAAAATCGAC
AAGGCGATGG AGTGTGTCTG GAAGCAGAAG CTATATGAGC CAGTGGTGAT CCCTGTGGGC
AAGCTGTTTC GACCAAATGA GAGACAAGTG GCTCAGCTGG CCAAGAAGGA TTACCTAGGC
AAGGCGACGG TCGTGGCATC TGTGGTGGAT CAAGACAGTT TCTCTGGGTT TGCCCCTCTG
CAACCACAGG CAGCTGAGCC TCCACCCAGA CCGAAAACCC CAGAGATCTT CAGGGCTCTG
GAAGGGGAGG CTCACCGTGT GCTATTTGGG TTTGTGCCTG AGACAAAAGA AGAGCTCCAG
GTCATGCCAG GGAACATTGT CTTTGTCTTG AAGAAGGGCA ATGATAACTG GGCCACGGTC
ATGTTCAACG GGCAGAAGGG GCTTGTTCCC TGCAACTACC TTGAACCAGT TGAGCTGCGG
ATCCACCCTC AGCAGCAGCC CCAGGAGGAA AGCTCTCCGC AGTCCGACAT CCCAGCTCCT
CCTAGTTCCA AAGCCCCTGG AAGACCCCAG CTGTCACCAG GCCAGAAACA AAAAGAAGAG
CCTAAGGAAG TGAAGCTCAG TGTTCCCATG CCCTACACAC TCAAGGTGCA CTACAAGTAC
ACGGTAGTCA TGAAGACTCA GCCCGGGCTC CCCTACAGCC AGGTCCGGGA CATGGTGTCT
AAGAAACTGG AGCTCCGGCT GGAACACACT AAGCTGAGCT ATCGGCCTCG GGACAGCAAT
GAGCTGGTGC CCCTTTCAGA AGACAGCATG AAGGATGCCT GGGGCCAGGT GAAAAACTAC
TGCCTGACTC TGTGGTGTGA GAACACAGTG GGTGACCAAG GCTTTCCAGA TGAACCCAAG
GAAAGTGAAA AAGCTGATGC TAATAACCAG ACAACAGAAC CTCAGCTTAA GAAAGGCAGC
CAAGTGGAGG CACTCTTCAG TTATGAGGCT ACCCAACCAG AGGACCTGGA GTTTCAGGAA
GGGGATATAA TCCTGGTGTT ATCAAAGGTG AATGAAGAAT GGCTGGAAGG GGAGTGCAAA
GGGAAGGTGG GCATTTTCCC CAAAGTTTTT GTTGAAGACT GCGCAACTAC AGATTTGGAA
AGCACTCGGA GAGAAGTCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu34408
Accession Version XM_005245207.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1473bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neutrophil cytosol factor 2 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTCCCTGG TGGAGGCCAT CAGCCTCTGG AATGAAGGGG TGCTGGCAGC GGACAAGAAG 
GACTGGAAGG GAGCCCTGGA TGCCTTCAGT GCCGTCCAGG ACCCCCACTC CCGGATTTGC
TTCAACATTG GCTGCATGTA CACTATCCTG AAGAACATGA CTGAAGCAGA GAAGGCCTTT
ACCAGAAGCA TTAACCGAGA CAAGCACTTG GCAGTGGCTT ACTTCCAACG AGGGATGCTC
TACTACCAGA CAGAGAAATA TGATTTGGCT ATCAAAGACC TTAAAGAAGC CTTGATTCAG
CTTCGAGGGA ACCAGCTGAT AGACTATAAG ATCCTGGGGC TCCAGTTCAA GCTGTTTGCC
TGTGAGGTGT TATATAACAT TGCTTTCATG TATGCCAAGA AGGAGGAATG GAAAAAAGCT
GAAGAACAGT TAGCATTGGC CACGAGCATG AAGTCTGAGC CCAGACATTC CAAAATCGAC
AAGGCGATGG AGTGTGTCTG GGTCGTGGCA TCTGTGGTGG ATCAAGACAG TTTCTCTGGG
TTTGCCCCTC TGCAACCACA GGCAGCTGAG CCTCCACCCA GACCGAAAAC CCCAGAGATC
TTCAGGGCTC TGGAAGGGGA GGCTCACCGT GTGCTATTTG GGTTTGTGCC TGAGACAAAA
GAAGAGCTCC AGGTCATGCC AGGGAACATT GTCTTTGTCT TGAAGAAGGG CAATGATAAC
TGGGCCACGG TCATGTTCAA CGGGCAGAAG GGGCTTGTTC CCTGCAACTA CCTTGAACCA
GTTGAGCTGC GGATCCACCC TCAGCAGCAG CCCCAGGAGG AAAGCTCTCC GCAGTCCGAC
ATCCCAGCTC CTCCTAGTTC CAAAGCCCCT GGAAGACCCC AGCTGTCACC AGGCCAGAAA
CAAAAAGAAG AGCCTAAGGA AGTGAAGCTC AGTGTTCCCA TGCCCTACAC ACTCAAGGTG
CACTACAAGT ACACGGTAGT CATGAAGACT CAGCCCGGGC TCCCCTACAG CCAGGTCCGG
GACATGGTGT CTAAGAAACT GGAGCTCCGG CTGGAACACA CTAAGCTGAG CTATCGGCCT
CGGGACAGCA ATGAGCTGGT GCCCCTTTCA GAAGACAGCA TGAAGGATGC CTGGGGCCAG
GTGAAAAACT ACTGCCTGAC TCTGTGGTGT GAGAACACAG TGGGTGACCA AGGCTTTCCA
GATGAACCCA AGGAAAGTGA AAAAGCTGAT GCTAATAACC AGACAACAGA ACCTCAGCTT
AAGAAAGGCA GCCAAGTGGA GGCACTCTTC AGTTATGAGG CTACCCAACC AGAGGACCTG
GAGTTTCAGG AAGGGGATAT AATCCTGGTG TTATCAAAGG TGAATGAAGA ATGGCTGGAA
GGGGAGTGCA AAGGGAAGGT GGGCATTTTC CCCAAAGTTT TTGTTGAAGA CTGCGCAACT
ACAGATTTGG AAAGCACTCG GAGAGAAGTC TAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005245264.1
CDS205..1677
Misc Feature(1)220..300(+)
Misc Feature(2)223..453(+)
Misc Feature(3)310..402(+)
Misc Feature(4)325..498(+)
Misc Feature(5)415..498(+)
Misc Feature(6)415..>498(+)
Misc Feature(7)826..987(+)
Misc Feature(8)841..975(+)
Misc Feature(9)1147..1383(+)
Misc Feature(10)1159..1350(+)
Misc Feature(11)1477..1635(+)
Misc Feature(12)1492..1626(+)
Translation
Position Chain Variation Link
17 17 a, c, g dbSNP:763929865
18 18 a, g dbSNP:550773018
25 25 c, t dbSNP:539242567
76 76 c, t dbSNP:34501573
82 82 c, t dbSNP:778540553
93 93 a, g dbSNP:754832075
105 105 a, c dbSNP:749130837
106 106 c, g dbSNP:780050246
107 107 c, t dbSNP:755614780
110 110 a, g dbSNP:184020650
111 111 a, g dbSNP:528747111
113 113 a, g dbSNP:767192295
116 116 -, gcca dbSNP:763900051
116 116 -, a dbSNP:35116326
123 123 c, t dbSNP:756941376
124 124 a, g dbSNP:751259507
127 127 c, t dbSNP:765474320
134 134 a, g dbSNP:759959096
136 136 g, t dbSNP:776937532
141 141 c, t dbSNP:561556995
142 142 a, g dbSNP:761230183
144 144 c, t dbSNP:549033920
159 159 c, g dbSNP:773251896
175 175 c, t dbSNP:772178602
177 177 a, c dbSNP:748277308
179 179 c, g dbSNP:774655949
188 188 c, t dbSNP:768273338
191 191 g, t dbSNP:749109356
193 193 c, t dbSNP:11540867
199 199 c, g dbSNP:780058052
201 201 a, t dbSNP:35681596
202 202 a, c dbSNP:745844166
205 205 a, c dbSNP:780810631
213 213 a, g dbSNP:756851268
216 216 a, g dbSNP:751114991
227 227 g, t dbSNP:368880633
244 244 g, t dbSNP:763816212
253 253 a, g dbSNP:758057222
254 254 a, c, t dbSNP:201589700
255 255 a, g dbSNP:761136334
265 265 g, t dbSNP:773175637
272 272 a, t dbSNP:767533151
278 278 a, c dbSNP:761962182
279 279 c, t dbSNP:774369490
293 293 a, g dbSNP:544745512
297 297 c, t dbSNP:143889676
298 298 a, g dbSNP:201869337
300 300 c, t dbSNP:769744791
305 305 a, g dbSNP:745752489
307 307 a, c dbSNP:368781381
312 312 c, t dbSNP:770284550
316 316 c, t dbSNP:200824291
317 317 a, g dbSNP:147415774
329 329 a, g dbSNP:137854514
334 334 c, g, t dbSNP:137854510
360 360 a, c, t dbSNP:752384353
379 379 a, c, g dbSNP:545984071
384 384 -, t dbSNP:760439163
397 397 a, g dbSNP:111532709
398 398 a, g dbSNP:778546412
400 400 a, c, t dbSNP:750782115
401 401 a, g, t dbSNP:142803799
405 405 c, t dbSNP:752044043
411 411 c, t dbSNP:764187192
425 425 a, g dbSNP:763140210
433 433 c, t dbSNP:752901695
434 434 a, g dbSNP:119103275
437 437 a, g dbSNP:137854519
439 439 a, g, t dbSNP:137854512
441 441 c, g dbSNP:770674754
447 447 c, t dbSNP:143178891
448 448 a, t dbSNP:201003183
455 455 c, t dbSNP:779088549
468 468 c, t dbSNP:770486258
483 483 c, g dbSNP:137854507
488 488 a, c dbSNP:765461476
494 494 c, g dbSNP:755222977
497 497 c, t dbSNP:754041676
500 500 c, t dbSNP:766146068
502 502 c, g, t dbSNP:119103276
504 504 a, g dbSNP:773131016
508 508 c, t dbSNP:374402066
509 509 a, c, g, t dbSNP:137854515
511 511 a, g dbSNP:768193963
512 512 a, g dbSNP:748898061
526 526 a, g dbSNP:749367579
527 527 a, t dbSNP:137854509
528 528 c, t dbSNP:775020616
536 536 c, t dbSNP:771262057
542 542 a, g dbSNP:200286542
546 546 c, g dbSNP:778259155
552 552 c, t dbSNP:758956466
561 561 c, t dbSNP:748707281
577 577 c, t dbSNP:773422870
578 578 a, g dbSNP:772598020
582 582 c, t dbSNP:748619343
587 587 c, t dbSNP:119103274
594 594 g, t dbSNP:779573048
600 600 c, g dbSNP:768786785
610 610 g, t dbSNP:749319711
613 613 a, t dbSNP:137854516
621 621 a, t dbSNP:780235098
623 623 a, c dbSNP:137854520
630 630 a, g dbSNP:779087160
640 640 c, t dbSNP:750697066
647 647 a, c, t dbSNP:764142541
648 648 a, g dbSNP:757064950
657 657 a, g dbSNP:751432737
661 661 a, g dbSNP:533500800
681 681 a, c, t dbSNP:369845453
683 683 a, t dbSNP:267606912
685 685 a, g dbSNP:137878529
688 688 a, g dbSNP:752127520
689 689 c, t dbSNP:376994104
690 690 a, g dbSNP:759074773
692 692 c, t dbSNP:372773130
696 696 a, g dbSNP:772503928
697 697 a, t dbSNP:762270172
700 700 c, g dbSNP:774918809
702 702 c, g dbSNP:769269175
708 708 c, t dbSNP:772322729
709 709 a, g dbSNP:761985515
713 713 c, t dbSNP:774160536
725 725 a, t dbSNP:768602664
726 726 a, t dbSNP:749187972
729 729 a, g dbSNP:780154260
732 732 c, t dbSNP:774027102
741 741 c, t dbSNP:745372047
757 757 c, t dbSNP:140028944
768 768 a, t dbSNP:758117639
769 769 c, g dbSNP:754192116
778 778 c, t dbSNP:780442146
786 786 a, c dbSNP:756610730
787 787 c, g dbSNP:750922183
788 788 c, t dbSNP:144451516
798 798 a, g dbSNP:757295495
799 799 a, g dbSNP:150595188
803 803 c, t dbSNP:764405660
812 812 c, g dbSNP:753556861
814 814 c, t dbSNP:766232480
820 820 a, g dbSNP:760561718
829 829 c, t dbSNP:750380443
831 831 c, t dbSNP:766952838
832 832 c, t dbSNP:761315970
833 833 a, g, t dbSNP:768181039
838 838 c, t dbSNP:762714261
855 855 c, t dbSNP:776822572
873 873 c, t dbSNP:771349846
880 880 a, t dbSNP:747462402
891 891 c, t dbSNP:778431195
893 893 c, t dbSNP:772775720
901 901 a, g dbSNP:748228594
903 903 c, t dbSNP:371493437
910 910 -, aag dbSNP:778751639
912 912 a, g dbSNP:557535861
916 916 a, g dbSNP:539632630
917 917 a, g dbSNP:754118806
932 932 a, c, t dbSNP:13306581
936 936 c, g dbSNP:554005946
945 945 c, t dbSNP:767437169
946 946 g, t dbSNP:761796267
972 972 c, g dbSNP:757931282
978 978 c, t dbSNP:752217634
983 983 a, c, g dbSNP:549428558
986 986 c, t dbSNP:35937854
991 991 c, t dbSNP:1126596
994 994 c, t dbSNP:768029053
995 995 a, g dbSNP:762173491
998 998 c, t dbSNP:771296779
1000 1000 -, c dbSNP:777621636
1006 1006 c, g dbSNP:774932046
1011 1011 c, g dbSNP:768549092
1014 1014 a, g dbSNP:535561432
1019 1019 a, g dbSNP:775489066
1023 1023 a, g dbSNP:112336681
1034 1034 c, t dbSNP:137937390
1035 1035 a, c, g dbSNP:556389548
1039 1039 c, t dbSNP:556255666
1041 1041 c, t dbSNP:200655126
1042 1042 a, g dbSNP:778521448
1044 1044 c, t dbSNP:754545169
1047 1047 c, t dbSNP:753471440
1054 1054 c, g, t dbSNP:755778188
1057 1057 a, c, t dbSNP:751928437
1060 1060 a, g dbSNP:764404690
1073 1073 c, t dbSNP:369515038
1075 1075 a, g dbSNP:138860204
1079 1079 a, g dbSNP:137854511
1081 1081 a, c dbSNP:765235671
1087 1087 c, t dbSNP:199658789
1089 1089 a, g dbSNP:776750922
1113 1113 -, aga dbSNP:757305871
1114 1114 a, g dbSNP:755689355
1141 1141 c, t dbSNP:745458881
1147 1147 c, t dbSNP:780702980
1148 1148 c, t dbSNP:571460195
1151 1151 a, g dbSNP:748489095
1165 1165 c, t dbSNP:765400770
1169 1169 a, g dbSNP:755434573
1170 1170 c, t dbSNP:546373985
1177 1177 a, t dbSNP:147744729
1179 1179 a, g dbSNP:756157300
1183 1183 a, g dbSNP:560823166
1199 1199 a, c dbSNP:767622954
1200 1200 c, g, t dbSNP:541838904
1201 1201 a, g dbSNP:137854513
1203 1203 a, g dbSNP:762936258
1206 1206 c, t dbSNP:775303126
1218 1218 c, g dbSNP:769837205
1222 1222 c, t dbSNP:777251055
1223 1223 a, g dbSNP:369205421
1229 1229 a, t dbSNP:776252831
1246 1246 a, g dbSNP:770537584
1252 1252 c, t dbSNP:746692621
1253 1253 a, g dbSNP:147908264
1261 1261 -, cacacta dbSNP:756393096
1261 1261 a, c dbSNP:201325416
1263 1263 a, c, t dbSNP:17849502
1274 1274 a, g dbSNP:756705355
1276 1276 g, t dbSNP:749606885
1278 1278 c, t dbSNP:538760999
1279 1279 a, c, g, t dbSNP:13306575
1280 1280 a, g dbSNP:145229115
1283 1283 a, c dbSNP:371503781
1285 1285 c, t dbSNP:145970396
1286 1286 a, g dbSNP:370251291
1287 1287 a, g dbSNP:758694739
1296 1296 c, t dbSNP:148005568
1297 1297 a, g dbSNP:765110090
1308 1308 c, t dbSNP:528239550
1315 1315 a, g dbSNP:753825493
1319 1319 a, g dbSNP:765806399
1321 1321 -, a dbSNP:759398660
1332 1332 g, t dbSNP:760169471
1338 1338 g, t dbSNP:377591562
1340 1340 -, g dbSNP:751478804
1352 1352 a, t dbSNP:35012521
1366 1366 -, ct dbSNP:765868844
1369 1369 c, t dbSNP:373876135
1376 1376 a, g dbSNP:775679393
1380 1380 c, g, t dbSNP:745334269
1390 1390 c, g dbSNP:774068321
1396 1396 c, g dbSNP:765454120
1412 1412 c, g dbSNP:534011480
1417 1417 a, g dbSNP:759940920
1419 1419 a, g dbSNP:777183751
1435 1435 a, g dbSNP:771532464
1436 1436 c, t dbSNP:747548282
1438 1438 a, g dbSNP:141569987
1455 1455 a, c dbSNP:772204296
1456 1456 c, t dbSNP:55761650
1460 1460 a, g dbSNP:142633260
1462 1462 a, c dbSNP:779188117
1469 1469 a, c dbSNP:755209791
1473 1473 c, t dbSNP:749065525
1497 1497 c, t dbSNP:779867537
1501 1501 a, g dbSNP:756008519
1506 1506 c, t dbSNP:750384875
1515 1515 a, g dbSNP:766880844
1521 1521 a, c dbSNP:756797744
1540 1540 a, g dbSNP:751096649
1542 1542 c, t dbSNP:146762552
1548 1548 c, t dbSNP:139817332
1553 1553 c, t dbSNP:762733492
1586 1586 a, g dbSNP:749546475
1587 1587 c, g dbSNP:556748175
1592 1592 a, g dbSNP:755918616
1597 1597 a, g dbSNP:745740745
1599 1599 a, g dbSNP:544453576
1611 1611 c, t dbSNP:757201201
1620 1620 -, a dbSNP:772569356
1627 1627 a, g dbSNP:376920441
1637 1637 a, g dbSNP:183100144
1638 1638 c, t dbSNP:769779675
1639 1639 a, g dbSNP:369006606
1648 1648 c, g dbSNP:143901397
1650 1650 g, t dbSNP:534490818
1658 1658 a, g dbSNP:374667798
1664 1664 a, g, t dbSNP:139108402
1669 1669 -, ga dbSNP:746336607
1672 1672 c, g dbSNP:762269733
1674 1674 c, t dbSNP:781431052
1677 1677 a, g dbSNP:768748386
1679 1679 a, c, t dbSNP:200240164
1684 1684 c, t dbSNP:370795833
1691 1691 c, g dbSNP:770071182
1699 1699 c, g dbSNP:745650507
1704 1704 a, g dbSNP:560754812
1707 1707 a, g dbSNP:780899104
1726 1726 c, g, t dbSNP:746942426
1730 1730 -, ttgt dbSNP:778645987
1735 1735 c, t dbSNP:536373941
1739 1739 -, taagatttag dbSNP:777040106
1742 1742 a, c dbSNP:770972754
1755 1755 c, t dbSNP:568830928
1761 1761 a, g dbSNP:187489898
1774 1774 a, c dbSNP:367946786
1793 1793 c, t dbSNP:532135800
1843 1843 g, t dbSNP:747039614
1893 1893 a, g dbSNP:571212408
1905 1905 c, g dbSNP:182819269
1924 1924 c, t dbSNP:777856745
1939 1939 c, t dbSNP:201050705
1951 1951 -, ttc dbSNP:34035622
1968 1968 -, gtt dbSNP:775582279
1997 1997 a, g dbSNP:528361273
1998 1998 -, acta dbSNP:776233541
1999 1999 -, cattca dbSNP:768663267
2003 2003 -, t dbSNP:746883471
2012 2012 -, a dbSNP:779658024
2035 2035 g, t dbSNP:578107077
2049 2049 -, gt dbSNP:202202602
2070 2070 -, a dbSNP:771465757
2075 2075 -, cat dbSNP:397951870
2075 2075 g, t dbSNP:796860
2075 2075 g, t, tcat dbSNP:369000587
2078 2078 -, cat dbSNP:61684650
2079 2079 -, ac, atc dbSNP:13306577
2080 2080 -, catct dbSNP:543935249
2122 2122 -, agta dbSNP:767143237
2135 2135 c, t dbSNP:758760667
2144 2144 c, t dbSNP:375774362
2157 2157 c, g dbSNP:563118156
2171 2171 g, t dbSNP:200423742
2189 2189 -, ggtta dbSNP:555649211
2194 2194 -, t dbSNP:573207861
2209 2209 g, t dbSNP:753309403
2219 2219 -, gttt dbSNP:757261466
2220 2220 -, gttt dbSNP:376681445
2223 2223 -, gttt dbSNP:767119882

Target ORF information:

RefSeq Version XM_005245207
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neutrophil cytosolic factor 2 (NCF2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005245207

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTCCCTGG TGGAGGCCAT CAGCCTCTGG AATGAAGGGG TGCTGGCAGC GGACAAGAAG 
GACTGGAAGG GAGCCCTGGA TGCCTTCAGT GCCGTCCAGG ACCCCCACTC CCGGATTTGC
TTCAACATTG GCTGCATGTA CACTATCCTG AAGAACATGA CTGAAGCAGA GAAGGCCTTT
ACCAGAAGCA TTAACCGAGA CAAGCACTTG GCAGTGGCTT ACTTCCAACG AGGGATGCTC
TACTACCAGA CAGAGAAATA TGATTTGGCT ATCAAAGACC TTAAAGAAGC CTTGATTCAG
CTTCGAGGGA ACCAGCTGAT AGACTATAAG ATCCTGGGGC TCCAGTTCAA GCTGTTTGCC
TGTGAGGTGT TATATAACAT TGCTTTCATG TATGCCAAGA AGGAGGAATG GAAAAAAGCT
GAAGAACAGT TAGCATTGGC CACGAGCATG AAGTCTGAGC CCAGACATTC CAAAATCGAC
AAGGCGATGG AGTGTGTCTG GGTCGTGGCA TCTGTGGTGG ATCAAGACAG TTTCTCTGGG
TTTGCCCCTC TGCAACCACA GGCAGCTGAG CCTCCACCCA GACCGAAAAC CCCAGAGATC
TTCAGGGCTC TGGAAGGGGA GGCTCACCGT GTGCTATTTG GGTTTGTGCC TGAGACAAAA
GAAGAGCTCC AGGTCATGCC AGGGAACATT GTCTTTGTCT TGAAGAAGGG CAATGATAAC
TGGGCCACGG TCATGTTCAA CGGGCAGAAG GGGCTTGTTC CCTGCAACTA CCTTGAACCA
GTTGAGCTGC GGATCCACCC TCAGCAGCAG CCCCAGGAGG AAAGCTCTCC GCAGTCCGAC
ATCCCAGCTC CTCCTAGTTC CAAAGCCCCT GGAAGACCCC AGCTGTCACC AGGCCAGAAA
CAAAAAGAAG AGCCTAAGGA AGTGAAGCTC AGTGTTCCCA TGCCCTACAC ACTCAAGGTG
CACTACAAGT ACACGGTAGT CATGAAGACT CAGCCCGGGC TCCCCTACAG CCAGGTCCGG
GACATGGTGT CTAAGAAACT GGAGCTCCGG CTGGAACACA CTAAGCTGAG CTATCGGCCT
CGGGACAGCA ATGAGCTGGT GCCCCTTTCA GAAGACAGCA TGAAGGATGC CTGGGGCCAG
GTGAAAAACT ACTGCCTGAC TCTGTGGTGT GAGAACACAG TGGGTGACCA AGGCTTTCCA
GATGAACCCA AGGAAAGTGA AAAAGCTGAT GCTAATAACC AGACAACAGA ACCTCAGCTT
AAGAAAGGCA GCCAAGTGGA GGCACTCTTC AGTTATGAGG CTACCCAACC AGAGGACCTG
GAGTTTCAGG AAGGGGATAT AATCCTGGTG TTATCAAAGG TGAATGAAGA ATGGCTGGAA
GGGGAGTGCA AAGGGAAGGT GGGCATTTTC CCCAAAGTTT TTGTTGAAGA CTGCGCAACT
ACAGATTTGG AAAGCACTCG GAGAGAAGTC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16749
Accession Version NM_001190789.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1338bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neutrophil cytosol factor 2 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC358267.1, AK296672.1, M32011.1 and BU617086.1. Summary: This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]. Transcript Variant: This variant (4) lacks in-frame two consecutive exons in the CDS, as compared to variant 1. The resulting isoform (3) lacks an internal segment, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296672.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTCCCTGG TGGAGGCCAT CAGCCTCTGG AATGAAGGGG TGCTGGCAGC GGACAAGAAG 
GACTGGAAGG GAGCCCTGGA TGCCTTCAGT GCCGTCCAGG ACCCCCACTC CCGGATTTGC
TTCAACATTG GCTGCATGTA CACTATCCTG AAGAACATGA CTGAAGCAGA GAAGGCCTTT
ACCAGAAGCA TTAACCGAGA CAAGCACTTG GCAGTGGCTT ACTTCCAACG AGGGATGCTC
TACTACCAGA CAGAGAAATA TGATTTGGCT ATCAAAGACC TTAAAGAAGC CTTGATTCAG
CTTCGAGGGA ACCAGCTGAT AGACTATAAG ATCCTGGGGC TCCAGTTCAA GCTGTTTGCC
TGTGAGGTCG TGGCATCTGT GGTGGATCAA GACAGTTTCT CTGGGTTTGC CCCTCTGCAA
CCACAGGCAG CTGAGCCTCC ACCCAGACCG AAAACCCCAG AGATCTTCAG GGCTCTGGAA
GGGGAGGCTC ACCGTGTGCT ATTTGGGTTT GTGCCTGAGA CAAAAGAAGA GCTCCAGGTC
ATGCCAGGGA ACATTGTCTT TGTCTTGAAG AAGGGCAATG ATAACTGGGC CACGGTCATG
TTCAACGGGC AGAAGGGGCT TGTTCCCTGC AACTACCTTG AACCAGTTGA GCTGCGGATC
CACCCTCAGC AGCAGCCCCA GGAGGAAAGC TCTCCGCAGT CCGACATCCC AGCTCCTCCT
AGTTCCAAAG CCCCTGGAAG ACCCCAGCTG TCACCAGGCC AGAAACAAAA AGAAGAGCCT
AAGGAAGTGA AGCTCAGTGT TCCCATGCCC TACACACTCA AGGTGCACTA CAAGTACACG
GTAGTCATGA AGACTCAGCC CGGGCTCCCC TACAGCCAGG TCCGGGACAT GGTGTCTAAG
AAACTGGAGC TCCGGCTGGA ACACACTAAG CTGAGCTATC GGCCTCGGGA CAGCAATGAG
CTGGTGCCCC TTTCAGAAGA CAGCATGAAG GATGCCTGGG GCCAGGTGAA AAACTACTGC
CTGACTCTGT GGTGTGAGAA CACAGTGGGT GACCAAGGCT TTCCAGATGA ACCCAAGGAA
AGTGAAAAAG CTGATGCTAA TAACCAGACA ACAGAACCTC AGCTTAAGAA AGGCAGCCAA
GTGGAGGCAC TCTTCAGTTA TGAGGCTACC CAACCAGAGG ACCTGGAGTT TCAGGAAGGG
GATATAATCC TGGTGTTATC AAAGGTGAAT GAAGAATGGC TGGAAGGGGA GTGCAAAGGG
AAGGTGGGCA TTTTCCCCAA AGTTTTTGTT GAAGACTGCG CAACTACAGA TTTGGAAAGC
ACTCGGAGAG AAGTCTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001177718.1
CDS276..1613
Misc Feature(1)189..191(+)
Misc Feature(2)291..371(+)
Misc Feature(3)294..524(+)
Misc Feature(4)381..473(+)
Misc Feature(5)396..569(+)
Misc Feature(6)486..569(+)
Misc Feature(7)486..>569(+)
Misc Feature(8)762..923(+)
Misc Feature(9)777..911(+)
Misc Feature(10)1083..1319(+)
Misc Feature(11)1095..1286(+)
Misc Feature(12)1413..1571(+)
Misc Feature(13)1428..1562(+)
Exon (1)1..449
Gene:NCF2
Gene Synonym:
Exon (2)450..532
Gene:NCF2
Gene Synonym:
Exon (3)533..641
Gene:NCF2
Gene Synonym:
Exon (4)642..701
Gene:NCF2
Gene Synonym:
Exon (5)702..745
Gene:NCF2
Gene Synonym:
Exon (6)746..887
Gene:NCF2
Gene Synonym:
Exon (7)888..956
Gene:NCF2
Gene Synonym:
Exon (8)957..1032
Gene:NCF2
Gene Synonym:
Exon (9)1033..1058
Gene:NCF2
Gene Synonym:
Exon (10)1059..1210
Gene:NCF2
Gene Synonym:
Exon (11)1211..1322
Gene:NCF2
Gene Synonym:
Exon (12)1323..1500
Gene:NCF2
Gene Synonym:
Exon (13)1501..2169
Gene:NCF2
Gene Synonym:
Translation
Position Chain Variation Link
12 12 c, t dbSNP:150182900
18 18 a, g dbSNP:574011269
27 27 -, a dbSNP:35066000
29 29 -, ctct dbSNP:771477568
29 29 -, ct dbSNP:768809741
36 36 g, t dbSNP:2274065
37 37 -, ctt dbSNP:199728796
49 49 c, t dbSNP:569365439
88 88 a, c, g dbSNP:763929865
89 89 a, g dbSNP:550773018
96 96 c, t dbSNP:539242567
147 147 c, t dbSNP:34501573
153 153 c, t dbSNP:778540553
164 164 a, g dbSNP:754832075
176 176 a, c dbSNP:749130837
177 177 c, g dbSNP:780050246
178 178 c, t dbSNP:755614780
181 181 a, g dbSNP:184020650
182 182 a, g dbSNP:528747111
184 184 a, g dbSNP:767192295
187 187 -, gcca dbSNP:763900051
187 187 -, a dbSNP:35116326
194 194 c, t dbSNP:756941376
195 195 a, g dbSNP:751259507
198 198 c, t dbSNP:765474320
205 205 a, g dbSNP:759959096
207 207 g, t dbSNP:776937532
212 212 c, t dbSNP:561556995
213 213 a, g dbSNP:761230183
215 215 c, t dbSNP:549033920
230 230 c, g dbSNP:773251896
246 246 c, t dbSNP:772178602
248 248 a, c dbSNP:748277308
250 250 c, g dbSNP:774655949
259 259 c, t dbSNP:768273338
262 262 g, t dbSNP:749109356
264 264 c, t dbSNP:11540867
270 270 c, g dbSNP:780058052
272 272 a, t dbSNP:35681596
273 273 a, c dbSNP:745844166
276 276 a, c dbSNP:780810631
284 284 a, g dbSNP:756851268
287 287 a, g dbSNP:751114991
298 298 g, t dbSNP:368880633
315 315 g, t dbSNP:763816212
324 324 a, g dbSNP:758057222
325 325 a, c, t dbSNP:201589700
326 326 a, g dbSNP:761136334
336 336 g, t dbSNP:773175637
343 343 a, t dbSNP:767533151
349 349 a, c dbSNP:761962182
350 350 c, t dbSNP:774369490
364 364 a, g dbSNP:544745512
368 368 c, t dbSNP:143889676
369 369 a, g dbSNP:201869337
371 371 c, t dbSNP:769744791
376 376 a, g dbSNP:745752489
378 378 a, c dbSNP:368781381
383 383 c, t dbSNP:770284550
387 387 c, t dbSNP:200824291
388 388 a, g dbSNP:147415774
400 400 a, g dbSNP:137854514
405 405 c, g, t dbSNP:137854510
431 431 a, c, t dbSNP:752384353
450 450 a, c, g dbSNP:545984071
455 455 -, t dbSNP:760439163
468 468 a, g dbSNP:111532709
469 469 a, g dbSNP:778546412
471 471 a, c, t dbSNP:750782115
472 472 a, g, t dbSNP:142803799
476 476 c, t dbSNP:752044043
482 482 c, t dbSNP:764187192
496 496 a, g dbSNP:763140210
504 504 c, t dbSNP:752901695
505 505 a, g dbSNP:119103275
508 508 a, g dbSNP:137854519
510 510 a, g, t dbSNP:137854512
512 512 c, g dbSNP:770674754
518 518 c, t dbSNP:143178891
519 519 a, t dbSNP:201003183
526 526 c, t dbSNP:779088549
539 539 c, t dbSNP:770486258
554 554 c, g dbSNP:137854507
559 559 a, c dbSNP:765461476
565 565 c, g dbSNP:755222977
568 568 c, t dbSNP:754041676
571 571 c, t dbSNP:766146068
573 573 c, g, t dbSNP:119103276
575 575 a, g dbSNP:773131016
579 579 c, t dbSNP:374402066
580 580 a, c, g, t dbSNP:137854515
582 582 a, g dbSNP:768193963
583 583 a, g dbSNP:748898061
597 597 a, g dbSNP:749367579
598 598 a, t dbSNP:137854509
599 599 c, t dbSNP:775020616
607 607 c, t dbSNP:771262057
613 613 a, g dbSNP:200286542
617 617 c, g dbSNP:778259155
623 623 c, t dbSNP:758956466
632 632 c, t dbSNP:748707281
644 644 c, t dbSNP:772322729
645 645 a, g dbSNP:761985515
649 649 c, t dbSNP:774160536
661 661 a, t dbSNP:768602664
662 662 a, t dbSNP:749187972
665 665 a, g dbSNP:780154260
668 668 c, t dbSNP:774027102
677 677 c, t dbSNP:745372047
693 693 c, t dbSNP:140028944
704 704 a, t dbSNP:758117639
705 705 c, g dbSNP:754192116
714 714 c, t dbSNP:780442146
722 722 a, c dbSNP:756610730
723 723 c, g dbSNP:750922183
724 724 c, t dbSNP:144451516
734 734 a, g dbSNP:757295495
735 735 a, g dbSNP:150595188
739 739 c, t dbSNP:764405660
748 748 c, g dbSNP:753556861
750 750 c, t dbSNP:766232480
756 756 a, g dbSNP:760561718
765 765 c, t dbSNP:750380443
767 767 c, t dbSNP:766952838
768 768 c, t dbSNP:761315970
769 769 a, g, t dbSNP:768181039
774 774 c, t dbSNP:762714261
791 791 c, t dbSNP:776822572
809 809 c, t dbSNP:771349846
816 816 a, t dbSNP:747462402
827 827 c, t dbSNP:778431195
829 829 c, t dbSNP:772775720
837 837 a, g dbSNP:748228594
839 839 c, t dbSNP:371493437
846 846 -, aag dbSNP:778751639
848 848 a, g dbSNP:557535861
852 852 a, g dbSNP:539632630
853 853 a, g dbSNP:754118806
868 868 a, c, t dbSNP:13306581
872 872 c, g dbSNP:554005946
881 881 c, t dbSNP:767437169
882 882 g, t dbSNP:761796267
908 908 c, g dbSNP:757931282
914 914 c, t dbSNP:752217634
919 919 a, c, g dbSNP:549428558
922 922 c, t dbSNP:35937854
927 927 c, t dbSNP:1126596
930 930 c, t dbSNP:768029053
931 931 a, g dbSNP:762173491
934 934 c, t dbSNP:771296779
936 936 -, c dbSNP:777621636
942 942 c, g dbSNP:774932046
947 947 c, g dbSNP:768549092
950 950 a, g dbSNP:535561432
955 955 a, g dbSNP:775489066
959 959 a, g dbSNP:112336681
970 970 c, t dbSNP:137937390
971 971 a, c, g dbSNP:556389548
975 975 c, t dbSNP:556255666
977 977 c, t dbSNP:200655126
978 978 a, g dbSNP:778521448
980 980 c, t dbSNP:754545169
983 983 c, t dbSNP:753471440
990 990 c, g, t dbSNP:755778188
993 993 a, c, t dbSNP:751928437
996 996 a, g dbSNP:764404690
1009 1009 c, t dbSNP:369515038
1011 1011 a, g dbSNP:138860204
1015 1015 a, g dbSNP:137854511
1017 1017 a, c dbSNP:765235671
1023 1023 c, t dbSNP:199658789
1025 1025 a, g dbSNP:776750922
1049 1049 -, aga dbSNP:757305871
1050 1050 a, g dbSNP:755689355
1077 1077 c, t dbSNP:745458881
1083 1083 c, t dbSNP:780702980
1084 1084 c, t dbSNP:571460195
1087 1087 a, g dbSNP:748489095
1101 1101 c, t dbSNP:765400770
1105 1105 a, g dbSNP:755434573
1106 1106 c, t dbSNP:546373985
1113 1113 a, t dbSNP:147744729
1115 1115 a, g dbSNP:756157300
1119 1119 a, g dbSNP:560823166
1135 1135 a, c dbSNP:767622954
1136 1136 c, g, t dbSNP:541838904
1137 1137 a, g dbSNP:137854513
1139 1139 a, g dbSNP:762936258
1142 1142 c, t dbSNP:775303126
1154 1154 c, g dbSNP:769837205
1158 1158 c, t dbSNP:777251055
1159 1159 a, g dbSNP:369205421
1165 1165 a, t dbSNP:776252831
1182 1182 a, g dbSNP:770537584
1188 1188 c, t dbSNP:746692621
1189 1189 a, g dbSNP:147908264
1197 1197 -, cacacta dbSNP:756393096
1197 1197 a, c dbSNP:201325416
1199 1199 a, c, t dbSNP:17849502
1210 1210 a, g dbSNP:756705355
1212 1212 g, t dbSNP:749606885
1214 1214 c, t dbSNP:538760999
1215 1215 a, c, g, t dbSNP:13306575
1216 1216 a, g dbSNP:145229115
1219 1219 a, c dbSNP:371503781
1221 1221 c, t dbSNP:145970396
1222 1222 a, g dbSNP:370251291
1223 1223 a, g dbSNP:758694739
1232 1232 c, t dbSNP:148005568
1233 1233 a, g dbSNP:765110090
1244 1244 c, t dbSNP:528239550
1251 1251 a, g dbSNP:753825493
1255 1255 a, g dbSNP:765806399
1257 1257 -, a dbSNP:759398660
1268 1268 g, t dbSNP:760169471
1274 1274 g, t dbSNP:377591562
1276 1276 -, g dbSNP:751478804
1288 1288 a, t dbSNP:35012521
1302 1302 -, ct dbSNP:765868844
1305 1305 c, t dbSNP:373876135
1312 1312 a, g dbSNP:775679393
1316 1316 c, g, t dbSNP:745334269
1326 1326 c, g dbSNP:774068321
1332 1332 c, g dbSNP:765454120
1348 1348 c, g dbSNP:534011480
1353 1353 a, g dbSNP:759940920
1355 1355 a, g dbSNP:777183751
1371 1371 a, g dbSNP:771532464
1372 1372 c, t dbSNP:747548282
1374 1374 a, g dbSNP:141569987
1391 1391 a, c dbSNP:772204296
1392 1392 c, t dbSNP:55761650
1396 1396 a, g dbSNP:142633260
1398 1398 a, c dbSNP:779188117
1405 1405 a, c dbSNP:755209791
1409 1409 c, t dbSNP:749065525
1433 1433 c, t dbSNP:779867537
1437 1437 a, g dbSNP:756008519
1442 1442 c, t dbSNP:750384875
1451 1451 a, g dbSNP:766880844
1457 1457 a, c dbSNP:756797744
1476 1476 a, g dbSNP:751096649
1478 1478 c, t dbSNP:146762552
1484 1484 c, t dbSNP:139817332
1489 1489 c, t dbSNP:762733492
1522 1522 a, g dbSNP:749546475
1523 1523 c, g dbSNP:556748175
1528 1528 a, g dbSNP:755918616
1533 1533 a, g dbSNP:745740745
1535 1535 a, g dbSNP:544453576
1547 1547 c, t dbSNP:757201201
1556 1556 -, a dbSNP:772569356
1563 1563 a, g dbSNP:376920441
1573 1573 a, g dbSNP:183100144
1574 1574 c, t dbSNP:769779675
1575 1575 a, g dbSNP:369006606
1584 1584 c, g dbSNP:143901397
1586 1586 g, t dbSNP:534490818
1594 1594 a, g dbSNP:374667798
1600 1600 a, g, t dbSNP:139108402
1605 1605 -, ga dbSNP:746336607
1608 1608 c, g dbSNP:762269733
1610 1610 c, t dbSNP:781431052
1613 1613 a, g dbSNP:768748386
1615 1615 a, c, t dbSNP:200240164
1620 1620 c, t dbSNP:370795833
1627 1627 c, g dbSNP:770071182
1635 1635 c, g dbSNP:745650507
1640 1640 a, g dbSNP:560754812
1643 1643 a, g dbSNP:780899104
1662 1662 c, g, t dbSNP:746942426
1666 1666 -, ttgt dbSNP:778645987
1671 1671 c, t dbSNP:536373941
1675 1675 -, taagatttag dbSNP:777040106
1678 1678 a, c dbSNP:770972754
1691 1691 c, t dbSNP:568830928
1697 1697 a, g dbSNP:187489898
1710 1710 a, c dbSNP:367946786
1729 1729 c, t dbSNP:532135800
1779 1779 g, t dbSNP:747039614
1829 1829 a, g dbSNP:571212408
1841 1841 c, g dbSNP:182819269
1860 1860 c, t dbSNP:777856745
1875 1875 c, t dbSNP:201050705
1887 1887 -, ttc dbSNP:34035622
1904 1904 -, gtt dbSNP:775582279
1933 1933 a, g dbSNP:528361273
1934 1934 -, acta dbSNP:776233541
1935 1935 -, cattca dbSNP:768663267
1939 1939 -, t dbSNP:746883471
1948 1948 -, a dbSNP:779658024
1971 1971 g, t dbSNP:578107077
1985 1985 -, gt dbSNP:202202602
2006 2006 -, a dbSNP:771465757
2011 2011 -, cat dbSNP:397951870
2011 2011 g, t dbSNP:796860
2011 2011 g, t, tcat dbSNP:369000587
2014 2014 -, cat dbSNP:61684650
2015 2015 -, ac, atc dbSNP:13306577
2016 2016 -, catct dbSNP:543935249
2058 2058 -, agta dbSNP:767143237
2071 2071 c, t dbSNP:758760667
2080 2080 c, t dbSNP:375774362
2093 2093 c, g dbSNP:563118156
2107 2107 g, t dbSNP:200423742
2125 2125 -, ggtta dbSNP:555649211
2130 2130 -, t dbSNP:573207861
2145 2145 g, t dbSNP:753309403
2155 2155 -, gttt dbSNP:757261466
2156 2156 -, gttt dbSNP:376681445
2159 2159 -, gttt dbSNP:767119882

Target ORF information:

RefSeq Version NM_001190789
Organism Homo sapiens (human)
Definition Homo sapiens neutrophil cytosolic factor 2 (NCF2), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001190789

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTCCCTGG TGGAGGCCAT CAGCCTCTGG AATGAAGGGG TGCTGGCAGC GGACAAGAAG 
GACTGGAAGG GAGCCCTGGA TGCCTTCAGT GCCGTCCAGG ACCCCCACTC CCGGATTTGC
TTCAACATTG GCTGCATGTA CACTATCCTG AAGAACATGA CTGAAGCAGA GAAGGCCTTT
ACCAGAAGCA TTAACCGAGA CAAGCACTTG GCAGTGGCTT ACTTCCAACG AGGGATGCTC
TACTACCAGA CAGAGAAATA TGATTTGGCT ATCAAAGACC TTAAAGAAGC CTTGATTCAG
CTTCGAGGGA ACCAGCTGAT AGACTATAAG ATCCTGGGGC TCCAGTTCAA GCTGTTTGCC
TGTGAGGTCG TGGCATCTGT GGTGGATCAA GACAGTTTCT CTGGGTTTGC CCCTCTGCAA
CCACAGGCAG CTGAGCCTCC ACCCAGACCG AAAACCCCAG AGATCTTCAG GGCTCTGGAA
GGGGAGGCTC ACCGTGTGCT ATTTGGGTTT GTGCCTGAGA CAAAAGAAGA GCTCCAGGTC
ATGCCAGGGA ACATTGTCTT TGTCTTGAAG AAGGGCAATG ATAACTGGGC CACGGTCATG
TTCAACGGGC AGAAGGGGCT TGTTCCCTGC AACTACCTTG AACCAGTTGA GCTGCGGATC
CACCCTCAGC AGCAGCCCCA GGAGGAAAGC TCTCCGCAGT CCGACATCCC AGCTCCTCCT
AGTTCCAAAG CCCCTGGAAG ACCCCAGCTG TCACCAGGCC AGAAACAAAA AGAAGAGCCT
AAGGAAGTGA AGCTCAGTGT TCCCATGCCC TACACACTCA AGGTGCACTA CAAGTACACG
GTAGTCATGA AGACTCAGCC CGGGCTCCCC TACAGCCAGG TCCGGGACAT GGTGTCTAAG
AAACTGGAGC TCCGGCTGGA ACACACTAAG CTGAGCTATC GGCCTCGGGA CAGCAATGAG
CTGGTGCCCC TTTCAGAAGA CAGCATGAAG GATGCCTGGG GCCAGGTGAA AAACTACTGC
CTGACTCTGT GGTGTGAGAA CACAGTGGGT GACCAAGGCT TTCCAGATGA ACCCAAGGAA
AGTGAAAAAG CTGATGCTAA TAACCAGACA ACAGAACCTC AGCTTAAGAA AGGCAGCCAA
GTGGAGGCAC TCTTCAGTTA TGAGGCTACC CAACCAGAGG ACCTGGAGTT TCAGGAAGGG
GATATAATCC TGGTGTTATC AAAGGTGAAT GAAGAATGGC TGGAAGGGGA GTGCAAAGGG
AAGGTGGGCA TTTTCCCCAA AGTTTTTGTT GAAGACTGCG CAACTACAGA TTTGGAAAGC
ACTCGGAGAG AAGTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19282
Accession Version NM_001127651.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1581bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neutrophil cytosol factor 2 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA817767.1, M32011.1, AL137800.12 and AW951155.1. On Jun 30, 2010 this sequence version replaced gi:189083741. Summary: This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M32011.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCCCTGG TGGAGGCCAT CAGCCTCTGG AATGAAGGGG TGCTGGCAGC GGACAAGAAG 
GACTGGAAGG GAGCCCTGGA TGCCTTCAGT GCCGTCCAGG ACCCCCACTC CCGGATTTGC
TTCAACATTG GCTGCATGTA CACTATCCTG AAGAACATGA CTGAAGCAGA GAAGGCCTTT
ACCAGAAGCA TTAACCGAGA CAAGCACTTG GCAGTGGCTT ACTTCCAACG AGGGATGCTC
TACTACCAGA CAGAGAAATA TGATTTGGCT ATCAAAGACC TTAAAGAAGC CTTGATTCAG
CTTCGAGGGA ACCAGCTGAT AGACTATAAG ATCCTGGGGC TCCAGTTCAA GCTGTTTGCC
TGTGAGGTGT TATATAACAT TGCTTTCATG TATGCCAAGA AGGAGGAATG GAAAAAAGCT
GAAGAACAGT TAGCATTGGC CACGAGCATG AAGTCTGAGC CCAGACATTC CAAAATCGAC
AAGGCGATGG AGTGTGTCTG GAAGCAGAAG CTATATGAGC CAGTGGTGAT CCCTGTGGGC
AAGCTGTTTC GACCAAATGA GAGACAAGTG GCTCAGCTGG CCAAGAAGGA TTACCTAGGC
AAGGCGACGG TCGTGGCATC TGTGGTGGAT CAAGACAGTT TCTCTGGGTT TGCCCCTCTG
CAACCACAGG CAGCTGAGCC TCCACCCAGA CCGAAAACCC CAGAGATCTT CAGGGCTCTG
GAAGGGGAGG CTCACCGTGT GCTATTTGGG TTTGTGCCTG AGACAAAAGA AGAGCTCCAG
GTCATGCCAG GGAACATTGT CTTTGTCTTG AAGAAGGGCA ATGATAACTG GGCCACGGTC
ATGTTCAACG GGCAGAAGGG GCTTGTTCCC TGCAACTACC TTGAACCAGT TGAGCTGCGG
ATCCACCCTC AGCAGCAGCC CCAGGAGGAA AGCTCTCCGC AGTCCGACAT CCCAGCTCCT
CCTAGTTCCA AAGCCCCTGG AAGACCCCAG CTGTCACCAG GCCAGAAACA AAAAGAAGAG
CCTAAGGAAG TGAAGCTCAG TGTTCCCATG CCCTACACAC TCAAGGTGCA CTACAAGTAC
ACGGTAGTCA TGAAGACTCA GCCCGGGCTC CCCTACAGCC AGGTCCGGGA CATGGTGTCT
AAGAAACTGG AGCTCCGGCT GGAACACACT AAGCTGAGCT ATCGGCCTCG GGACAGCAAT
GAGCTGGTGC CCCTTTCAGA AGACAGCATG AAGGATGCCT GGGGCCAGGT GAAAAACTAC
TGCCTGACTC TGTGGTGTGA GAACACAGTG GGTGACCAAG GCTTTCCAGA TGAACCCAAG
GAAAGTGAAA AAGCTGATGC TAATAACCAG ACAACAGAAC CTCAGCTTAA GAAAGGCAGC
CAAGTGGAGG CACTCTTCAG TTATGAGGCT ACCCAACCAG AGGACCTGGA GTTTCAGGAA
GGGGATATAA TCCTGGTGTT ATCAAAGGTG AATGAAGAAT GGCTGGAAGG GGAGTGCAAA
GGGAAGGTGG GCATTTTCCC CAAAGTTTTT GTTGAAGACT GCGCAACTAC AGATTTGGAA
AGCACTCGGA GAGAAGTCTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001121123.1
CDS113..1693
Misc Feature(1)47..49(+)
Misc Feature(2)128..208(+)
Misc Feature(3)131..361(+)
Misc Feature(4)218..310(+)
Misc Feature(5)221..322(+)
Misc Feature(6)233..406(+)
Misc Feature(7)323..424(+)
Misc Feature(8)323..406(+)
Misc Feature(9)323..>406(+)
Misc Feature(10)473..574(+)
Misc Feature(11)842..1003(+)
Misc Feature(12)857..991(+)
Misc Feature(13)1163..1399(+)
Misc Feature(14)1175..1366(+)
Misc Feature(15)1493..1651(+)
Misc Feature(16)1508..1642(+)
Exon (1)1..82
Gene:NCF2
Gene Synonym:
Exon (2)83..286
Gene:NCF2
Gene Synonym:
Exon (3)287..369
Gene:NCF2
Gene Synonym:
Exon (4)370..478
Gene:NCF2
Gene Synonym:
Exon (5)479..613
Gene:NCF2
Gene Synonym:
Exon (6)614..721
Gene:NCF2
Gene Synonym:
Exon (7)722..781
Gene:NCF2
Gene Synonym:
Exon (8)782..825
Gene:NCF2
Gene Synonym:
Exon (9)826..967
Gene:NCF2
Gene Synonym:
Exon (10)968..1036
Gene:NCF2
Gene Synonym:
Exon (11)1037..1112
Gene:NCF2
Gene Synonym:
Exon (12)1113..1138
Gene:NCF2
Gene Synonym:
Exon (13)1139..1290
Gene:NCF2
Gene Synonym:
Exon (14)1291..1402
Gene:NCF2
Gene Synonym:
Exon (15)1403..1580
Gene:NCF2
Gene Synonym:
Exon (16)1581..2249
Gene:NCF2
Gene Synonym:
Translation
Position Chain Variation Link
1 1 a, t dbSNP:542643175
12 12 c, g dbSNP:188693904
22 22 c, t dbSNP:13306580
59 59 -, c dbSNP:35552352
77 77 -, t dbSNP:71555411
83 83 c, t dbSNP:772178602
85 85 a, c dbSNP:748277308
87 87 c, g dbSNP:774655949
96 96 c, t dbSNP:768273338
99 99 g, t dbSNP:749109356
101 101 c, t dbSNP:11540867
107 107 c, g dbSNP:780058052
109 109 a, t dbSNP:35681596
110 110 a, c dbSNP:745844166
113 113 a, c dbSNP:780810631
121 121 a, g dbSNP:756851268
124 124 a, g dbSNP:751114991
135 135 g, t dbSNP:368880633
152 152 g, t dbSNP:763816212
161 161 a, g dbSNP:758057222
162 162 a, c, t dbSNP:201589700
163 163 a, g dbSNP:761136334
173 173 g, t dbSNP:773175637
180 180 a, t dbSNP:767533151
186 186 a, c dbSNP:761962182
187 187 c, t dbSNP:774369490
201 201 a, g dbSNP:544745512
205 205 c, t dbSNP:143889676
206 206 a, g dbSNP:201869337
208 208 c, t dbSNP:769744791
213 213 a, g dbSNP:745752489
215 215 a, c dbSNP:368781381
220 220 c, t dbSNP:770284550
224 224 c, t dbSNP:200824291
225 225 a, g dbSNP:147415774
237 237 a, g dbSNP:137854514
242 242 c, g, t dbSNP:137854510
268 268 a, c, t dbSNP:752384353
287 287 a, c, g dbSNP:545984071
292 292 -, t dbSNP:760439163
305 305 a, g dbSNP:111532709
306 306 a, g dbSNP:778546412
308 308 a, c, t dbSNP:750782115
309 309 a, g, t dbSNP:142803799
313 313 c, t dbSNP:752044043
319 319 c, t dbSNP:764187192
333 333 a, g dbSNP:763140210
341 341 c, t dbSNP:752901695
342 342 a, g dbSNP:119103275
345 345 a, g dbSNP:137854519
347 347 a, g, t dbSNP:137854512
349 349 c, g dbSNP:770674754
355 355 c, t dbSNP:143178891
356 356 a, t dbSNP:201003183
363 363 c, t dbSNP:779088549
376 376 c, t dbSNP:770486258
391 391 c, g dbSNP:137854507
396 396 a, c dbSNP:765461476
402 402 c, g dbSNP:755222977
405 405 c, t dbSNP:754041676
408 408 c, t dbSNP:766146068
410 410 c, g, t dbSNP:119103276
412 412 a, g dbSNP:773131016
416 416 c, t dbSNP:374402066
417 417 a, c, g, t dbSNP:137854515
419 419 a, g dbSNP:768193963
420 420 a, g dbSNP:748898061
434 434 a, g dbSNP:749367579
435 435 a, t dbSNP:137854509
436 436 c, t dbSNP:775020616
444 444 c, t dbSNP:771262057
450 450 a, g dbSNP:200286542
454 454 c, g dbSNP:778259155
460 460 c, t dbSNP:758956466
469 469 c, t dbSNP:748707281
485 485 c, t dbSNP:773422870
486 486 a, g dbSNP:772598020
490 490 c, t dbSNP:748619343
495 495 c, t dbSNP:119103274
502 502 g, t dbSNP:779573048
508 508 c, g dbSNP:768786785
518 518 g, t dbSNP:749319711
521 521 a, t dbSNP:137854516
529 529 a, t dbSNP:780235098
531 531 a, c dbSNP:137854520
538 538 a, g dbSNP:779087160
548 548 c, t dbSNP:750697066
555 555 a, c, t dbSNP:764142541
556 556 a, g dbSNP:757064950
565 565 a, g dbSNP:751432737
569 569 a, g dbSNP:533500800
589 589 a, c, t dbSNP:369845453
591 591 a, t dbSNP:267606912
593 593 a, g dbSNP:137878529
596 596 a, g dbSNP:752127520
597 597 c, t dbSNP:376994104
598 598 a, g dbSNP:759074773
600 600 c, t dbSNP:372773130
604 604 a, g dbSNP:772503928
605 605 a, t dbSNP:762270172
608 608 c, g dbSNP:774918809
610 610 c, g dbSNP:769269175
617 617 c, g dbSNP:137854517
620 620 a, g dbSNP:769704327
623 623 c, t dbSNP:145354685
625 625 a, g, t dbSNP:771120104
632 632 c, t dbSNP:747272727
637 637 a, g dbSNP:777507234
640 640 a, g dbSNP:758239702
654 654 a, g dbSNP:2274064
658 658 a, g dbSNP:778911214
662 662 c, t dbSNP:766745748
663 663 c, g dbSNP:137854518
675 675 a, g dbSNP:115365142
677 677 a, c dbSNP:755796920
678 678 a, g dbSNP:750131635
686 686 c, g dbSNP:764314608
698 698 -, aag dbSNP:773850705
700 700 g, t dbSNP:554848021
707 707 a, c dbSNP:763388019
717 717 c, t dbSNP:137854508
718 718 a, g dbSNP:17849501
719 719 a, g dbSNP:765762727
720 720 c, t dbSNP:760085363
721 721 a, g dbSNP:147657171
724 724 c, t dbSNP:772322729
725 725 a, g dbSNP:761985515
729 729 c, t dbSNP:774160536
741 741 a, t dbSNP:768602664
742 742 a, t dbSNP:749187972
745 745 a, g dbSNP:780154260
748 748 c, t dbSNP:774027102
757 757 c, t dbSNP:745372047
773 773 c, t dbSNP:140028944
784 784 a, t dbSNP:758117639
785 785 c, g dbSNP:754192116
794 794 c, t dbSNP:780442146
802 802 a, c dbSNP:756610730
803 803 c, g dbSNP:750922183
804 804 c, t dbSNP:144451516
814 814 a, g dbSNP:757295495
815 815 a, g dbSNP:150595188
819 819 c, t dbSNP:764405660
828 828 c, g dbSNP:753556861
830 830 c, t dbSNP:766232480
836 836 a, g dbSNP:760561718
845 845 c, t dbSNP:750380443
847 847 c, t dbSNP:766952838
848 848 c, t dbSNP:761315970
849 849 a, g, t dbSNP:768181039
854 854 c, t dbSNP:762714261
871 871 c, t dbSNP:776822572
889 889 c, t dbSNP:771349846
896 896 a, t dbSNP:747462402
907 907 c, t dbSNP:778431195
909 909 c, t dbSNP:772775720
917 917 a, g dbSNP:748228594
919 919 c, t dbSNP:371493437
926 926 -, aag dbSNP:778751639
928 928 a, g dbSNP:557535861
932 932 a, g dbSNP:539632630
933 933 a, g dbSNP:754118806
948 948 a, c, t dbSNP:13306581
952 952 c, g dbSNP:554005946
961 961 c, t dbSNP:767437169
962 962 g, t dbSNP:761796267
988 988 c, g dbSNP:757931282
994 994 c, t dbSNP:752217634
999 999 a, c, g dbSNP:549428558
1002 1002 c, t dbSNP:35937854
1007 1007 c, t dbSNP:1126596
1010 1010 c, t dbSNP:768029053
1011 1011 a, g dbSNP:762173491
1014 1014 c, t dbSNP:771296779
1016 1016 -, c dbSNP:777621636
1022 1022 c, g dbSNP:774932046
1027 1027 c, g dbSNP:768549092
1030 1030 a, g dbSNP:535561432
1035 1035 a, g dbSNP:775489066
1039 1039 a, g dbSNP:112336681
1050 1050 c, t dbSNP:137937390
1051 1051 a, c, g dbSNP:556389548
1055 1055 c, t dbSNP:556255666
1057 1057 c, t dbSNP:200655126
1058 1058 a, g dbSNP:778521448
1060 1060 c, t dbSNP:754545169
1063 1063 c, t dbSNP:753471440
1070 1070 c, g, t dbSNP:755778188
1073 1073 a, c, t dbSNP:751928437
1076 1076 a, g dbSNP:764404690
1089 1089 c, t dbSNP:369515038
1091 1091 a, g dbSNP:138860204
1095 1095 a, g dbSNP:137854511
1097 1097 a, c dbSNP:765235671
1103 1103 c, t dbSNP:199658789
1105 1105 a, g dbSNP:776750922
1129 1129 -, aga dbSNP:757305871
1130 1130 a, g dbSNP:755689355
1157 1157 c, t dbSNP:745458881
1163 1163 c, t dbSNP:780702980
1164 1164 c, t dbSNP:571460195
1167 1167 a, g dbSNP:748489095
1181 1181 c, t dbSNP:765400770
1185 1185 a, g dbSNP:755434573
1186 1186 c, t dbSNP:546373985
1193 1193 a, t dbSNP:147744729
1195 1195 a, g dbSNP:756157300
1199 1199 a, g dbSNP:560823166
1215 1215 a, c dbSNP:767622954
1216 1216 c, g, t dbSNP:541838904
1217 1217 a, g dbSNP:137854513
1219 1219 a, g dbSNP:762936258
1222 1222 c, t dbSNP:775303126
1234 1234 c, g dbSNP:769837205
1238 1238 c, t dbSNP:777251055
1239 1239 a, g dbSNP:369205421
1245 1245 a, t dbSNP:776252831
1262 1262 a, g dbSNP:770537584
1268 1268 c, t dbSNP:746692621
1269 1269 a, g dbSNP:147908264
1277 1277 -, cacacta dbSNP:756393096
1277 1277 a, c dbSNP:201325416
1279 1279 a, c, t dbSNP:17849502
1290 1290 a, g dbSNP:756705355
1292 1292 g, t dbSNP:749606885
1294 1294 c, t dbSNP:538760999
1295 1295 a, c, g, t dbSNP:13306575
1296 1296 a, g dbSNP:145229115
1299 1299 a, c dbSNP:371503781
1301 1301 c, t dbSNP:145970396
1302 1302 a, g dbSNP:370251291
1303 1303 a, g dbSNP:758694739
1312 1312 c, t dbSNP:148005568
1313 1313 a, g dbSNP:765110090
1324 1324 c, t dbSNP:528239550
1331 1331 a, g dbSNP:753825493
1335 1335 a, g dbSNP:765806399
1337 1337 -, a dbSNP:759398660
1348 1348 g, t dbSNP:760169471
1354 1354 g, t dbSNP:377591562
1356 1356 -, g dbSNP:751478804
1368 1368 a, t dbSNP:35012521
1382 1382 -, ct dbSNP:765868844
1385 1385 c, t dbSNP:373876135
1392 1392 a, g dbSNP:775679393
1396 1396 c, g, t dbSNP:745334269
1406 1406 c, g dbSNP:774068321
1412 1412 c, g dbSNP:765454120
1428 1428 c, g dbSNP:534011480
1433 1433 a, g dbSNP:759940920
1435 1435 a, g dbSNP:777183751
1451 1451 a, g dbSNP:771532464
1452 1452 c, t dbSNP:747548282
1454 1454 a, g dbSNP:141569987
1471 1471 a, c dbSNP:772204296
1472 1472 c, t dbSNP:55761650
1476 1476 a, g dbSNP:142633260
1478 1478 a, c dbSNP:779188117
1485 1485 a, c dbSNP:755209791
1489 1489 c, t dbSNP:749065525
1513 1513 c, t dbSNP:779867537
1517 1517 a, g dbSNP:756008519
1522 1522 c, t dbSNP:750384875
1531 1531 a, g dbSNP:766880844
1537 1537 a, c dbSNP:756797744
1556 1556 a, g dbSNP:751096649
1558 1558 c, t dbSNP:146762552
1564 1564 c, t dbSNP:139817332
1569 1569 c, t dbSNP:762733492
1602 1602 a, g dbSNP:749546475
1603 1603 c, g dbSNP:556748175
1608 1608 a, g dbSNP:755918616
1613 1613 a, g dbSNP:745740745
1615 1615 a, g dbSNP:544453576
1627 1627 c, t dbSNP:757201201
1636 1636 -, a dbSNP:772569356
1643 1643 a, g dbSNP:376920441
1653 1653 a, g dbSNP:183100144
1654 1654 c, t dbSNP:769779675
1655 1655 a, g dbSNP:369006606
1664 1664 c, g dbSNP:143901397
1666 1666 g, t dbSNP:534490818
1674 1674 a, g dbSNP:374667798
1680 1680 a, g, t dbSNP:139108402
1685 1685 -, ga dbSNP:746336607
1688 1688 c, g dbSNP:762269733
1690 1690 c, t dbSNP:781431052
1693 1693 a, g dbSNP:768748386
1695 1695 a, c, t dbSNP:200240164
1700 1700 c, t dbSNP:370795833
1707 1707 c, g dbSNP:770071182
1715 1715 c, g dbSNP:745650507
1720 1720 a, g dbSNP:560754812
1723 1723 a, g dbSNP:780899104
1742 1742 c, g, t dbSNP:746942426
1746 1746 -, ttgt dbSNP:778645987
1751 1751 c, t dbSNP:536373941
1755 1755 -, taagatttag dbSNP:777040106
1758 1758 a, c dbSNP:770972754
1771 1771 c, t dbSNP:568830928
1777 1777 a, g dbSNP:187489898
1790 1790 a, c dbSNP:367946786
1809 1809 c, t dbSNP:532135800
1859 1859 g, t dbSNP:747039614
1909 1909 a, g dbSNP:571212408
1921 1921 c, g dbSNP:182819269
1940 1940 c, t dbSNP:777856745
1955 1955 c, t dbSNP:201050705
1967 1967 -, ttc dbSNP:34035622
1984 1984 -, gtt dbSNP:775582279
2013 2013 a, g dbSNP:528361273
2014 2014 -, acta dbSNP:776233541
2015 2015 -, cattca dbSNP:768663267
2019 2019 -, t dbSNP:746883471
2028 2028 -, a dbSNP:779658024
2051 2051 g, t dbSNP:578107077
2065 2065 -, gt dbSNP:202202602
2086 2086 -, a dbSNP:771465757
2091 2091 -, cat dbSNP:397951870
2091 2091 g, t dbSNP:796860
2091 2091 g, t, tcat dbSNP:369000587
2094 2094 -, cat dbSNP:61684650
2095 2095 -, ac, atc dbSNP:13306577
2096 2096 -, catct dbSNP:543935249
2138 2138 -, agta dbSNP:767143237
2151 2151 c, t dbSNP:758760667
2160 2160 c, t dbSNP:375774362
2173 2173 c, g dbSNP:563118156
2187 2187 g, t dbSNP:200423742
2205 2205 -, ggtta dbSNP:555649211
2210 2210 -, t dbSNP:573207861
2225 2225 g, t dbSNP:753309403
2235 2235 -, gttt dbSNP:757261466
2236 2236 -, gttt dbSNP:376681445
2239 2239 -, gttt dbSNP:767119882

Target ORF information:

RefSeq Version NM_001127651
Organism Homo sapiens (human)
Definition Homo sapiens neutrophil cytosolic factor 2 (NCF2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127651

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCCCTGG TGGAGGCCAT CAGCCTCTGG AATGAAGGGG TGCTGGCAGC GGACAAGAAG 
GACTGGAAGG GAGCCCTGGA TGCCTTCAGT GCCGTCCAGG ACCCCCACTC CCGGATTTGC
TTCAACATTG GCTGCATGTA CACTATCCTG AAGAACATGA CTGAAGCAGA GAAGGCCTTT
ACCAGAAGCA TTAACCGAGA CAAGCACTTG GCAGTGGCTT ACTTCCAACG AGGGATGCTC
TACTACCAGA CAGAGAAATA TGATTTGGCT ATCAAAGACC TTAAAGAAGC CTTGATTCAG
CTTCGAGGGA ACCAGCTGAT AGACTATAAG ATCCTGGGGC TCCAGTTCAA GCTGTTTGCC
TGTGAGGTGT TATATAACAT TGCTTTCATG TATGCCAAGA AGGAGGAATG GAAAAAAGCT
GAAGAACAGT TAGCATTGGC CACGAGCATG AAGTCTGAGC CCAGACATTC CAAAATCGAC
AAGGCGATGG AGTGTGTCTG GAAGCAGAAG CTATATGAGC CAGTGGTGAT CCCTGTGGGC
AAGCTGTTTC GACCAAATGA GAGACAAGTG GCTCAGCTGG CCAAGAAGGA TTACCTAGGC
AAGGCGACGG TCGTGGCATC TGTGGTGGAT CAAGACAGTT TCTCTGGGTT TGCCCCTCTG
CAACCACAGG CAGCTGAGCC TCCACCCAGA CCGAAAACCC CAGAGATCTT CAGGGCTCTG
GAAGGGGAGG CTCACCGTGT GCTATTTGGG TTTGTGCCTG AGACAAAAGA AGAGCTCCAG
GTCATGCCAG GGAACATTGT CTTTGTCTTG AAGAAGGGCA ATGATAACTG GGCCACGGTC
ATGTTCAACG GGCAGAAGGG GCTTGTTCCC TGCAACTACC TTGAACCAGT TGAGCTGCGG
ATCCACCCTC AGCAGCAGCC CCAGGAGGAA AGCTCTCCGC AGTCCGACAT CCCAGCTCCT
CCTAGTTCCA AAGCCCCTGG AAGACCCCAG CTGTCACCAG GCCAGAAACA AAAAGAAGAG
CCTAAGGAAG TGAAGCTCAG TGTTCCCATG CCCTACACAC TCAAGGTGCA CTACAAGTAC
ACGGTAGTCA TGAAGACTCA GCCCGGGCTC CCCTACAGCC AGGTCCGGGA CATGGTGTCT
AAGAAACTGG AGCTCCGGCT GGAACACACT AAGCTGAGCT ATCGGCCTCG GGACAGCAAT
GAGCTGGTGC CCCTTTCAGA AGACAGCATG AAGGATGCCT GGGGCCAGGT GAAAAACTAC
TGCCTGACTC TGTGGTGTGA GAACACAGTG GGTGACCAAG GCTTTCCAGA TGAACCCAAG
GAAAGTGAAA AAGCTGATGC TAATAACCAG ACAACAGAAC CTCAGCTTAA GAAAGGCAGC
CAAGTGGAGG CACTCTTCAG TTATGAGGCT ACCCAACCAG AGGACCTGGA GTTTCAGGAA
GGGGATATAA TCCTGGTGTT ATCAAAGGTG AATGAAGAAT GGCTGGAAGG GGAGTGCAAA
GGGAAGGTGG GCATTTTCCC CAAAGTTTTT GTTGAAGACT GCGCAACTAC AGATTTGGAA
AGCACTCGGA GAGAAGTCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23215
Accession Version NM_001190794.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1446bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neutrophil cytosol factor 2 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC358267.1, AK298713.1, AL137800.12 and AW951155.1. Summary: This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]. Transcript Variant: This variant (3) lacks an in-frame exon in the CDS, as compared to variant 1. The resulting isoform (2) lacks an internal segment, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298713.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTCCCTGG TGGAGGCCAT CAGCCTCTGG AATGAAGGGG TGCTGGCAGC GGACAAGAAG 
GACTGGAAGG GAGCCCTGGA TGCCTTCAGT GCCGTCCAGG ACCCCCACTC CCGGATTTGC
TTCAACATTG GCTGCATGTA CACTATCCTG AAGAACATGA CTGAAGCAGA GAAGGCCTTT
ACCAGAAGCA TTAACCGAGA CAAGCACTTG GCAGTGGCTT ACTTCCAACG AGGGATGCTC
TACTACCAGA CAGAGAAATA TGATTTGGCT ATCAAAGACC TTAAAGAAGC CTTGATTCAG
CTTCGAGGGA ACCAGCTGAT AGACTATAAG ATCCTGGGGC TCCAGTTCAA GCTGTTTGCC
TGTGAGAAGC AGAAGCTATA TGAGCCAGTG GTGATCCCTG TGGGCAAGCT GTTTCGACCA
AATGAGAGAC AAGTGGCTCA GCTGGCCAAG AAGGATTACC TAGGCAAGGC GACGGTCGTG
GCATCTGTGG TGGATCAAGA CAGTTTCTCT GGGTTTGCCC CTCTGCAACC ACAGGCAGCT
GAGCCTCCAC CCAGACCGAA AACCCCAGAG ATCTTCAGGG CTCTGGAAGG GGAGGCTCAC
CGTGTGCTAT TTGGGTTTGT GCCTGAGACA AAAGAAGAGC TCCAGGTCAT GCCAGGGAAC
ATTGTCTTTG TCTTGAAGAA GGGCAATGAT AACTGGGCCA CGGTCATGTT CAACGGGCAG
AAGGGGCTTG TTCCCTGCAA CTACCTTGAA CCAGTTGAGC TGCGGATCCA CCCTCAGCAG
CAGCCCCAGG AGGAAAGCTC TCCGCAGTCC GACATCCCAG CTCCTCCTAG TTCCAAAGCC
CCTGGAAGAC CCCAGCTGTC ACCAGGCCAG AAACAAAAAG AAGAGCCTAA GGAAGTGAAG
CTCAGTGTTC CCATGCCCTA CACACTCAAG GTGCACTACA AGTACACGGT AGTCATGAAG
ACTCAGCCCG GGCTCCCCTA CAGCCAGGTC CGGGACATGG TGTCTAAGAA ACTGGAGCTC
CGGCTGGAAC ACACTAAGCT GAGCTATCGG CCTCGGGACA GCAATGAGCT GGTGCCCCTT
TCAGAAGACA GCATGAAGGA TGCCTGGGGC CAGGTGAAAA ACTACTGCCT GACTCTGTGG
TGTGAGAACA CAGTGGGTGA CCAAGGCTTT CCAGATGAAC CCAAGGAAAG TGAAAAAGCT
GATGCTAATA ACCAGACAAC AGAACCTCAG CTTAAGAAAG GCAGCCAAGT GGAGGCACTC
TTCAGTTATG AGGCTACCCA ACCAGAGGAC CTGGAGTTTC AGGAAGGGGA TATAATCCTG
GTGTTATCAA AGGTGAATGA AGAATGGCTG GAAGGGGAGT GCAAAGGGAA GGTGGGCATT
TTCCCCAAAG TTTTTGTTGA AGACTGCGCA ACTACAGATT TGGAAAGCAC TCGGAGAGAA
GTCTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001177723.1
CDS276..1721
Misc Feature(1)189..191(+)
Misc Feature(2)291..371(+)
Misc Feature(3)294..524(+)
Misc Feature(4)381..473(+)
Misc Feature(5)396..569(+)
Misc Feature(6)486..569(+)
Misc Feature(7)486..>569(+)
Misc Feature(8)870..1031(+)
Misc Feature(9)885..1019(+)
Misc Feature(10)1191..1427(+)
Misc Feature(11)1203..1394(+)
Misc Feature(12)1521..1679(+)
Misc Feature(13)1536..1670(+)
Exon (1)1..449
Gene:NCF2
Gene Synonym:
Exon (2)450..532
Gene:NCF2
Gene Synonym:
Exon (3)533..641
Gene:NCF2
Gene Synonym:
Exon (4)642..749
Gene:NCF2
Gene Synonym:
Exon (5)750..809
Gene:NCF2
Gene Synonym:
Exon (6)810..853
Gene:NCF2
Gene Synonym:
Exon (7)854..995
Gene:NCF2
Gene Synonym:
Exon (8)996..1064
Gene:NCF2
Gene Synonym:
Exon (9)1065..1140
Gene:NCF2
Gene Synonym:
Exon (10)1141..1166
Gene:NCF2
Gene Synonym:
Exon (11)1167..1318
Gene:NCF2
Gene Synonym:
Exon (12)1319..1430
Gene:NCF2
Gene Synonym:
Exon (13)1431..1608
Gene:NCF2
Gene Synonym:
Exon (14)1609..2277
Gene:NCF2
Gene Synonym:
Translation
Position Chain Variation Link
12 12 c, t dbSNP:150182900
18 18 a, g dbSNP:574011269
27 27 -, a dbSNP:35066000
29 29 -, ctct dbSNP:771477568
29 29 -, ct dbSNP:768809741
36 36 g, t dbSNP:2274065
37 37 -, ctt dbSNP:199728796
49 49 c, t dbSNP:569365439
88 88 a, c, g dbSNP:763929865
89 89 a, g dbSNP:550773018
96 96 c, t dbSNP:539242567
147 147 c, t dbSNP:34501573
153 153 c, t dbSNP:778540553
164 164 a, g dbSNP:754832075
176 176 a, c dbSNP:749130837
177 177 c, g dbSNP:780050246
178 178 c, t dbSNP:755614780
181 181 a, g dbSNP:184020650
182 182 a, g dbSNP:528747111
184 184 a, g dbSNP:767192295
187 187 -, gcca dbSNP:763900051
187 187 -, a dbSNP:35116326
194 194 c, t dbSNP:756941376
195 195 a, g dbSNP:751259507
198 198 c, t dbSNP:765474320
205 205 a, g dbSNP:759959096
207 207 g, t dbSNP:776937532
212 212 c, t dbSNP:561556995
213 213 a, g dbSNP:761230183
215 215 c, t dbSNP:549033920
230 230 c, g dbSNP:773251896
246 246 c, t dbSNP:772178602
248 248 a, c dbSNP:748277308
250 250 c, g dbSNP:774655949
259 259 c, t dbSNP:768273338
262 262 g, t dbSNP:749109356
264 264 c, t dbSNP:11540867
270 270 c, g dbSNP:780058052
272 272 a, t dbSNP:35681596
273 273 a, c dbSNP:745844166
276 276 a, c dbSNP:780810631
284 284 a, g dbSNP:756851268
287 287 a, g dbSNP:751114991
298 298 g, t dbSNP:368880633
315 315 g, t dbSNP:763816212
324 324 a, g dbSNP:758057222
325 325 a, c, t dbSNP:201589700
326 326 a, g dbSNP:761136334
336 336 g, t dbSNP:773175637
343 343 a, t dbSNP:767533151
349 349 a, c dbSNP:761962182
350 350 c, t dbSNP:774369490
364 364 a, g dbSNP:544745512
368 368 c, t dbSNP:143889676
369 369 a, g dbSNP:201869337
371 371 c, t dbSNP:769744791
376 376 a, g dbSNP:745752489
378 378 a, c dbSNP:368781381
383 383 c, t dbSNP:770284550
387 387 c, t dbSNP:200824291
388 388 a, g dbSNP:147415774
400 400 a, g dbSNP:137854514
405 405 c, g, t dbSNP:137854510
431 431 a, c, t dbSNP:752384353
450 450 a, c, g dbSNP:545984071
455 455 -, t dbSNP:760439163
468 468 a, g dbSNP:111532709
469 469 a, g dbSNP:778546412
471 471 a, c, t dbSNP:750782115
472 472 a, g, t dbSNP:142803799
476 476 c, t dbSNP:752044043
482 482 c, t dbSNP:764187192
496 496 a, g dbSNP:763140210
504 504 c, t dbSNP:752901695
505 505 a, g dbSNP:119103275
508 508 a, g dbSNP:137854519
510 510 a, g, t dbSNP:137854512
512 512 c, g dbSNP:770674754
518 518 c, t dbSNP:143178891
519 519 a, t dbSNP:201003183
526 526 c, t dbSNP:779088549
539 539 c, t dbSNP:770486258
554 554 c, g dbSNP:137854507
559 559 a, c dbSNP:765461476
565 565 c, g dbSNP:755222977
568 568 c, t dbSNP:754041676
571 571 c, t dbSNP:766146068
573 573 c, g, t dbSNP:119103276
575 575 a, g dbSNP:773131016
579 579 c, t dbSNP:374402066
580 580 a, c, g, t dbSNP:137854515
582 582 a, g dbSNP:768193963
583 583 a, g dbSNP:748898061
597 597 a, g dbSNP:749367579
598 598 a, t dbSNP:137854509
599 599 c, t dbSNP:775020616
607 607 c, t dbSNP:771262057
613 613 a, g dbSNP:200286542
617 617 c, g dbSNP:778259155
623 623 c, t dbSNP:758956466
632 632 c, t dbSNP:748707281
645 645 c, g dbSNP:137854517
648 648 a, g dbSNP:769704327
651 651 c, t dbSNP:145354685
653 653 a, g, t dbSNP:771120104
660 660 c, t dbSNP:747272727
665 665 a, g dbSNP:777507234
668 668 a, g dbSNP:758239702
682 682 a, g dbSNP:2274064
686 686 a, g dbSNP:778911214
690 690 c, t dbSNP:766745748
691 691 c, g dbSNP:137854518
703 703 a, g dbSNP:115365142
705 705 a, c dbSNP:755796920
706 706 a, g dbSNP:750131635
714 714 c, g dbSNP:764314608
726 726 -, aag dbSNP:773850705
728 728 g, t dbSNP:554848021
735 735 a, c dbSNP:763388019
745 745 c, t dbSNP:137854508
746 746 a, g dbSNP:17849501
747 747 a, g dbSNP:765762727
748 748 c, t dbSNP:760085363
749 749 a, g dbSNP:147657171
752 752 c, t dbSNP:772322729
753 753 a, g dbSNP:761985515
757 757 c, t dbSNP:774160536
769 769 a, t dbSNP:768602664
770 770 a, t dbSNP:749187972
773 773 a, g dbSNP:780154260
776 776 c, t dbSNP:774027102
785 785 c, t dbSNP:745372047
801 801 c, t dbSNP:140028944
812 812 a, t dbSNP:758117639
813 813 c, g dbSNP:754192116
822 822 c, t dbSNP:780442146
830 830 a, c dbSNP:756610730
831 831 c, g dbSNP:750922183
832 832 c, t dbSNP:144451516
842 842 a, g dbSNP:757295495
843 843 a, g dbSNP:150595188
847 847 c, t dbSNP:764405660
856 856 c, g dbSNP:753556861
858 858 c, t dbSNP:766232480
864 864 a, g dbSNP:760561718
873 873 c, t dbSNP:750380443
875 875 c, t dbSNP:766952838
876 876 c, t dbSNP:761315970
877 877 a, g, t dbSNP:768181039
882 882 c, t dbSNP:762714261
899 899 c, t dbSNP:776822572
917 917 c, t dbSNP:771349846
924 924 a, t dbSNP:747462402
935 935 c, t dbSNP:778431195
937 937 c, t dbSNP:772775720
945 945 a, g dbSNP:748228594
947 947 c, t dbSNP:371493437
954 954 -, aag dbSNP:778751639
956 956 a, g dbSNP:557535861
960 960 a, g dbSNP:539632630
961 961 a, g dbSNP:754118806
976 976 a, c, t dbSNP:13306581
980 980 c, g dbSNP:554005946
989 989 c, t dbSNP:767437169
990 990 g, t dbSNP:761796267
1016 1016 c, g dbSNP:757931282
1022 1022 c, t dbSNP:752217634
1027 1027 a, c, g dbSNP:549428558
1030 1030 c, t dbSNP:35937854
1035 1035 c, t dbSNP:1126596
1038 1038 c, t dbSNP:768029053
1039 1039 a, g dbSNP:762173491
1042 1042 c, t dbSNP:771296779
1044 1044 -, c dbSNP:777621636
1050 1050 c, g dbSNP:774932046
1055 1055 c, g dbSNP:768549092
1058 1058 a, g dbSNP:535561432
1063 1063 a, g dbSNP:775489066
1067 1067 a, g dbSNP:112336681
1078 1078 c, t dbSNP:137937390
1079 1079 a, c, g dbSNP:556389548
1083 1083 c, t dbSNP:556255666
1085 1085 c, t dbSNP:200655126
1086 1086 a, g dbSNP:778521448
1088 1088 c, t dbSNP:754545169
1091 1091 c, t dbSNP:753471440
1098 1098 c, g, t dbSNP:755778188
1101 1101 a, c, t dbSNP:751928437
1104 1104 a, g dbSNP:764404690
1117 1117 c, t dbSNP:369515038
1119 1119 a, g dbSNP:138860204
1123 1123 a, g dbSNP:137854511
1125 1125 a, c dbSNP:765235671
1131 1131 c, t dbSNP:199658789
1133 1133 a, g dbSNP:776750922
1157 1157 -, aga dbSNP:757305871
1158 1158 a, g dbSNP:755689355
1185 1185 c, t dbSNP:745458881
1191 1191 c, t dbSNP:780702980
1192 1192 c, t dbSNP:571460195
1195 1195 a, g dbSNP:748489095
1209 1209 c, t dbSNP:765400770
1213 1213 a, g dbSNP:755434573
1214 1214 c, t dbSNP:546373985
1221 1221 a, t dbSNP:147744729
1223 1223 a, g dbSNP:756157300
1227 1227 a, g dbSNP:560823166
1243 1243 a, c dbSNP:767622954
1244 1244 c, g, t dbSNP:541838904
1245 1245 a, g