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NCF4 cDNA ORF clone, Homo sapiens (human)

Gene Symbol NCF4
Entrez Gene ID 4689
Full Name neutrophil cytosolic factor 4, 40kDa
Synonyms NCF, P40PHOX, SH3PXD4
General protein information
Preferred Names
neutrophil cytosol factor 4
Names
neutrophil cytosol factor 4
NCF-4
p40-phox
neutrophil NADPH oxidase factor 4
SH3 and PX domain-containing protein 4
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

22

22q13.1

Summary The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

601488


mRNA and Protein(s)

mRNA Protein Name
XM_011530198 XP_011528500 neutrophil cytosol factor 4 isoform X1
XM_011530199 XP_011528501 neutrophil cytosol factor 4 isoform X2
NM_013416 NP_038202 neutrophil cytosol factor 4 isoform 2
NM_000631 NP_000622 neutrophil cytosol factor 4 isoform 1



Homo sapiens (human) NCF4 NP_000622.2
Pan troglodytes (chimpanzee) NCF4 XP_001159332.2
Canis lupus familiaris (dog) NCF4 XP_538398.2
Bos taurus (cattle) NCF4 NP_001039448.1
Mus musculus (house mouse) Ncf4 NP_032703.2
Rattus norvegicus (Norway rat) Ncf4 NP_001120776.1
Gallus gallus (chicken) NCF4 NP_001034361.1
Danio rerio (zebrafish) ncf4 NP_991274.2
Xenopus (Silurana) tropicalis (western clawed frog) ncf4 NP_001025692.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu59223 XM_011530198 PREDICTED: Homo sapiens neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu59224 XM_011530199 PREDICTED: Homo sapiens neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu18835 NM_013416 Homo sapiens neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu16465 NM_000631 Homo sapiens neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu59223
Clone ID Related Accession (Same CDS sequence) XM_011530198
Accession Version XM_011530198.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1194bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neutrophil cytosol factor 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGGGGCTC CCTGTACCTC GAAGGTCTTA TCTGAAAGCG AGCCTGGATT GTTCTGCTTA 
AGGTCAAAGC AGAGAGTACA AGGAGAGGGC GCAGGTTCGC GCAGCGGGGA CTTTCTAGAA
CCCCAAGACG ACGGTAGTGG CCGTGTATTG AGCAGTACTG AGCACCTACT GTATGCCGGG
CTCTGTTCTG GGCACTGGGA TGTAGGTGAC TTTGAACAGC TTCCGGATGA TGTTGCCATC
TCGGCCAACA TTGCTGACAT CGAGGAGAAG AGAGGCTTCA CCAGCCACTT TGTTTTCGTC
ATCGAGGTGA AGACAAAAGG AGGATCCAAG TACCTCATCT ACCGCCGCTA CCGCCAGTTC
CATGCTTTGC AGAGCAAGCT GGAGGAGCGC TTCGGGCCAG ACAGCAAGAG CAGTGCCCTG
GCCTGTACCC TGCCCACACT CCCAGCCAAA GTCTACGTGG GTGTGAAACA GGAGATCGCC
GAGATGCGGA TACCTGCCCT CAACGCCTAC ATGAAGAGCC TGCTCAGCCT GCCGGTCTGG
GTGCTGATGG ATGAGGACGT CCGGATCTTC TTTTACCAGT CGCCCTATGA CTCAGAGCAG
GTGCCCCAGG CACTCCGCCG GCTCCGCCCG CGCACCCGGA AAGTCAAGAG CGTGTCCCCA
CAGGGCAACA GCGTTGACCG CATGGCAGCT CCGAGAGCAG AGGCTCTATT TGACTTCACT
GGAAACAGCA AACTGGAGCT GAATTTCAAA GCTGGAGATG TGATCTTCCT CCTCAGTCGG
ATCAACAAAG ACTGGCTGGA GGGCACTGTC CGGGGAGCCA CGGGCATCTT CCCTCTCTCC
TTCGTGAAGA TCCTCAAAGA CTTCCCTGAG GAGGACGACC CCACCAACTG GCTGCGTTGC
TACTACTACG AAGACACCAT CAGCACCATC AAGGACATCG CGGTGGAGGA AGATCTCAGC
AGCACTCCCC TATTGAAAGA CCTGCTGGAG CTCACAAGGC GGGAGTTCCA GAGAGAGGAC
ATAGCTCTGA ATTACCGGGA CGCTGAGGGG GATCTGGTTC GGCTGCTGTC GGATGAGGAC
GTAGCGCTCA TGGTGCGGCA GGCTCGTGGC CTCCCCTCCC AGAAGCGCCT CTTCCCCTGG
AAGCTGCACA TCACGCAGAA GGACAACTAC AGGGTCTACA ACACGATGCC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528500.1
CDS793..1986
Misc Feature(1)1021..1389(+)
Misc Feature(2)1063..1389(+)
Misc Feature(3)1138..1281(+)
Misc Feature(4)1486..1647(+)
Misc Feature(5)1501..1635(+)
Misc Feature(6)1564..1614(+)
Misc Feature(7)1678..1953(+)
Misc Feature(8)1735..1932(+)
Misc Feature(9)1831..1872(+)
Translation

Target ORF information:

RefSeq Version XM_011530198
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011530198

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGGGGCTC CCTGTACCTC GAAGGTCTTA TCTGAAAGCG AGCCTGGATT GTTCTGCTTA 
AGGTCAAAGC AGAGAGTACA AGGAGAGGGC GCAGGTTCGC GCAGCGGGGA CTTTCTAGAA
CCCCAAGACG ACGGTAGTGG CCGTGTATTG AGCAGTACTG AGCACCTACT GTATGCCGGG
CTCTGTTCTG GGCACTGGGA TGTAGGTGAC TTTGAACAGC TTCCGGATGA TGTTGCCATC
TCGGCCAACA TTGCTGACAT CGAGGAGAAG AGAGGCTTCA CCAGCCACTT TGTTTTCGTC
ATCGAGGTGA AGACAAAAGG AGGATCCAAG TACCTCATCT ACCGCCGCTA CCGCCAGTTC
CATGCTTTGC AGAGCAAGCT GGAGGAGCGC TTCGGGCCAG ACAGCAAGAG CAGTGCCCTG
GCCTGTACCC TGCCCACACT CCCAGCCAAA GTCTACGTGG GTGTGAAACA GGAGATCGCC
GAGATGCGGA TACCTGCCCT CAACGCCTAC ATGAAGAGCC TGCTCAGCCT GCCGGTCTGG
GTGCTGATGG ATGAGGACGT CCGGATCTTC TTTTACCAGT CGCCCTATGA CTCAGAGCAG
GTGCCCCAGG CACTCCGCCG GCTCCGCCCG CGCACCCGGA AAGTCAAGAG CGTGTCCCCA
CAGGGCAACA GCGTTGACCG CATGGCAGCT CCGAGAGCAG AGGCTCTATT TGACTTCACT
GGAAACAGCA AACTGGAGCT GAATTTCAAA GCTGGAGATG TGATCTTCCT CCTCAGTCGG
ATCAACAAAG ACTGGCTGGA GGGCACTGTC CGGGGAGCCA CGGGCATCTT CCCTCTCTCC
TTCGTGAAGA TCCTCAAAGA CTTCCCTGAG GAGGACGACC CCACCAACTG GCTGCGTTGC
TACTACTACG AAGACACCAT CAGCACCATC AAGGACATCG CGGTGGAGGA AGATCTCAGC
AGCACTCCCC TATTGAAAGA CCTGCTGGAG CTCACAAGGC GGGAGTTCCA GAGAGAGGAC
ATAGCTCTGA ATTACCGGGA CGCTGAGGGG GATCTGGTTC GGCTGCTGTC GGATGAGGAC
GTAGCGCTCA TGGTGCGGCA GGCTCGTGGC CTCCCCTCCC AGAAGCGCCT CTTCCCCTGG
AAGCTGCACA TCACGCAGAA GGACAACTAC AGGGTCTACA ACACGATGCC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59224
Clone ID Related Accession (Same CDS sequence) XM_011530199
Accession Version XM_011530199.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1164bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neutrophil cytosol factor 4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCTCATTT ATTATTTTTT CTTGAACGTT GGCTCCCCCA TGAGAAGCAA GCTCCACGAG 
GCTGTGTCCG TTTTGATCGC TGCTGTATCA CTAGGAGACG ACGGTAGTGG CCGTGTATTG
AGCAGTACTG AGCACCTACT GTATGCCGGG CTCTGTTCTG GGCACTGGGA TGTAGGTGAC
TTTGAACAGC TTCCGGATGA TGTTGCCATC TCGGCCAACA TTGCTGACAT CGAGGAGAAG
AGAGGCTTCA CCAGCCACTT TGTTTTCGTC ATCGAGGTGA AGACAAAAGG AGGATCCAAG
TACCTCATCT ACCGCCGCTA CCGCCAGTTC CATGCTTTGC AGAGCAAGCT GGAGGAGCGC
TTCGGGCCAG ACAGCAAGAG CAGTGCCCTG GCCTGTACCC TGCCCACACT CCCAGCCAAA
GTCTACGTGG GTGTGAAACA GGAGATCGCC GAGATGCGGA TACCTGCCCT CAACGCCTAC
ATGAAGAGCC TGCTCAGCCT GCCGGTCTGG GTGCTGATGG ATGAGGACGT CCGGATCTTC
TTTTACCAGT CGCCCTATGA CTCAGAGCAG GTGCCCCAGG CACTCCGCCG GCTCCGCCCG
CGCACCCGGA AAGTCAAGAG CGTGTCCCCA CAGGGCAACA GCGTTGACCG CATGGCAGCT
CCGAGAGCAG AGGCTCTATT TGACTTCACT GGAAACAGCA AACTGGAGCT GAATTTCAAA
GCTGGAGATG TGATCTTCCT CCTCAGTCGG ATCAACAAAG ACTGGCTGGA GGGCACTGTC
CGGGGAGCCA CGGGCATCTT CCCTCTCTCC TTCGTGAAGA TCCTCAAAGA CTTCCCTGAG
GAGGACGACC CCACCAACTG GCTGCGTTGC TACTACTACG AAGACACCAT CAGCACCATC
AAGGACATCG CGGTGGAGGA AGATCTCAGC AGCACTCCCC TATTGAAAGA CCTGCTGGAG
CTCACAAGGC GGGAGTTCCA GAGAGAGGAC ATAGCTCTGA ATTACCGGGA CGCTGAGGGG
GATCTGGTTC GGCTGCTGTC GGATGAGGAC GTAGCGCTCA TGGTGCGGCA GGCTCGTGGC
CTCCCCTCCC AGAAGCGCCT CTTCCCCTGG AAGCTGCACA TCACGCAGAA GGACAACTAC
AGGGTCTACA ACACGATGCC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528501.1
CDS489..1652
Misc Feature(1)687..1055(+)
Misc Feature(2)729..1055(+)
Misc Feature(3)804..947(+)
Misc Feature(4)1152..1313(+)
Misc Feature(5)1167..1301(+)
Misc Feature(6)1230..1280(+)
Misc Feature(7)1344..1619(+)
Misc Feature(8)1401..1598(+)
Misc Feature(9)1497..1538(+)
Translation

Target ORF information:

RefSeq Version XM_011530199
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011530199

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCTCATTT ATTATTTTTT CTTGAACGTT GGCTCCCCCA TGAGAAGCAA GCTCCACGAG 
GCTGTGTCCG TTTTGATCGC TGCTGTATCA CTAGGAGACG ACGGTAGTGG CCGTGTATTG
AGCAGTACTG AGCACCTACT GTATGCCGGG CTCTGTTCTG GGCACTGGGA TGTAGGTGAC
TTTGAACAGC TTCCGGATGA TGTTGCCATC TCGGCCAACA TTGCTGACAT CGAGGAGAAG
AGAGGCTTCA CCAGCCACTT TGTTTTCGTC ATCGAGGTGA AGACAAAAGG AGGATCCAAG
TACCTCATCT ACCGCCGCTA CCGCCAGTTC CATGCTTTGC AGAGCAAGCT GGAGGAGCGC
TTCGGGCCAG ACAGCAAGAG CAGTGCCCTG GCCTGTACCC TGCCCACACT CCCAGCCAAA
GTCTACGTGG GTGTGAAACA GGAGATCGCC GAGATGCGGA TACCTGCCCT CAACGCCTAC
ATGAAGAGCC TGCTCAGCCT GCCGGTCTGG GTGCTGATGG ATGAGGACGT CCGGATCTTC
TTTTACCAGT CGCCCTATGA CTCAGAGCAG GTGCCCCAGG CACTCCGCCG GCTCCGCCCG
CGCACCCGGA AAGTCAAGAG CGTGTCCCCA CAGGGCAACA GCGTTGACCG CATGGCAGCT
CCGAGAGCAG AGGCTCTATT TGACTTCACT GGAAACAGCA AACTGGAGCT GAATTTCAAA
GCTGGAGATG TGATCTTCCT CCTCAGTCGG ATCAACAAAG ACTGGCTGGA GGGCACTGTC
CGGGGAGCCA CGGGCATCTT CCCTCTCTCC TTCGTGAAGA TCCTCAAAGA CTTCCCTGAG
GAGGACGACC CCACCAACTG GCTGCGTTGC TACTACTACG AAGACACCAT CAGCACCATC
AAGGACATCG CGGTGGAGGA AGATCTCAGC AGCACTCCCC TATTGAAAGA CCTGCTGGAG
CTCACAAGGC GGGAGTTCCA GAGAGAGGAC ATAGCTCTGA ATTACCGGGA CGCTGAGGGG
GATCTGGTTC GGCTGCTGTC GGATGAGGAC GTAGCGCTCA TGGTGCGGCA GGCTCGTGGC
CTCCCCTCCC AGAAGCGCCT CTTCCCCTGG AAGCTGCACA TCACGCAGAA GGACAACTAC
AGGGTCTACA ACACGATGCC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18835
Clone ID Related Accession (Same CDS sequence) NM_013416
Accession Version NM_013416.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1047bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neutrophil cytosol factor 4 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC002798.1, AK290924.1 and BU076892.1. This sequence is a reference standard in the RefSeqGene project. On Dec 20, 2007 this sequence version replaced gi:47519769. Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). This isoform (2) lacks the PC (Phox and Cdc24p) motif, which may be important for the interaction with NCF2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002798.1, AK290924.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTGTGG CCCAGCAGCT GCGGGCCGAG AGTGACTTTG AACAGCTTCC GGATGATGTT 
GCCATCTCGG CCAACATTGC TGACATCGAG GAGAAGAGAG GCTTCACCAG CCACTTTGTT
TTCGTCATCG AGGTGAAGAC AAAAGGAGGA TCCAAGTACC TCATCTACCG CCGCTACCGC
CAGTTCCATG CTTTGCAGAG CAAGCTGGAG GAGCGCTTCG GGCCAGACAG CAAGAGCAGT
GCCCTGGCCT GTACCCTGCC CACACTCCCA GCCAAAGTCT ACGTGGGTGT GAAACAGGAG
ATCGCCGAGA TGCGGATACC TGCCCTCAAC GCCTACATGA AGAGCCTGCT CAGCCTGCCG
GTCTGGGTGC TGATGGATGA GGACGTCCGG ATCTTCTTTT ACCAGTCGCC CTATGACTCA
GAGCAGGTGC CCCAGGCACT CCGCCGGCTC CGCCCGCGCA CCCGGAAAGT CAAGAGCGTG
TCCCCACAGG GCAACAGCGT TGACCGCATG GCAGCTCCGA GAGCAGAGGC TCTATTTGAC
TTCACTGGAA ACAGCAAACT GGAGCTGAAT TTCAAAGCTG GAGATGTGAT CTTCCTCCTC
AGTCGGATCA ACAAAGACTG GCTGGAGGGC ACTGTCCGGG GAGCCACGGG CATCTTCCCT
CTCTCCTTCG TGAAGATCCT CAAAGACTTC CCTGAGGAGG ACGACCCCAC CAACTGGCTG
CGTTGCTACT ACTACGAAGA CACCATCAGC ACCATCAAGT CTGTGGCCTG GGAGGGAGGG
GCCTGTCCAG CCTTCCTGCC ATCCCTACGA CCACTGCCCC TCACATCACC TTCTCATGGG
TCCCTCTCCC ACTCCAAAGC CCCCAGTGGC TCCCAGATGA GCCACAATGC TGTAACAAGC
CATCAACGTC CAGGGTGGCC TGGCCAGCCT CATTCCCCTT TCCCCCACCC CACACCCCAC
TTCCAGCCTG ATGCCTCCTT ACTCCAGCCT GTCACCCCCT TAGGGACATC GCGGTGGAGG
AAGATCTCAG CAGCACTCCC CTATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_038202.2
CDS185..1231
Misc Feature(1)122..124(+)
Misc Feature(2)239..607(+)
Misc Feature(3)281..607(+)
Misc Feature(4)356..499(+)
Misc Feature(5)356..364(+)
Misc Feature(6)458..466(+)
Misc Feature(7)644..646(+)
Misc Feature(8)704..865(+)
Misc Feature(9)719..853(+)
Misc Feature(10)782..832(+)
Misc Feature(11)896..>958(+)
Misc Feature(12)899..946(+)
Misc Feature(13)899..943(+)
Misc Feature(14)905..907(+)
Exon (1)1..216
Gene:NCF4
Gene Synonym:
Exon (2)217..301
Gene:NCF4
Gene Synonym:
Exon (3)302..455
Gene:NCF4
Gene Synonym:
Exon (4)456..526
Gene:NCF4
Gene Synonym:
Exon (5)527..654
Gene:NCF4
Gene Synonym:
Exon (6)655..712
Gene:NCF4
Gene Synonym:
Exon (7)713..811
Gene:NCF4
Gene Synonym:
Exon (8)812..1253
Gene:NCF4
Gene Synonym:
Exon (9)1254..1643
Gene:NCF4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_013416
Organism Homo sapiens (human)
Definition Homo sapiens neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_013416

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTGTGG CCCAGCAGCT GCGGGCCGAG AGTGACTTTG AACAGCTTCC GGATGATGTT 
GCCATCTCGG CCAACATTGC TGACATCGAG GAGAAGAGAG GCTTCACCAG CCACTTTGTT
TTCGTCATCG AGGTGAAGAC AAAAGGAGGA TCCAAGTACC TCATCTACCG CCGCTACCGC
CAGTTCCATG CTTTGCAGAG CAAGCTGGAG GAGCGCTTCG GGCCAGACAG CAAGAGCAGT
GCCCTGGCCT GTACCCTGCC CACACTCCCA GCCAAAGTCT ACGTGGGTGT GAAACAGGAG
ATCGCCGAGA TGCGGATACC TGCCCTCAAC GCCTACATGA AGAGCCTGCT CAGCCTGCCG
GTCTGGGTGC TGATGGATGA GGACGTCCGG ATCTTCTTTT ACCAGTCGCC CTATGACTCA
GAGCAGGTGC CCCAGGCACT CCGCCGGCTC CGCCCGCGCA CCCGGAAAGT CAAGAGCGTG
TCCCCACAGG GCAACAGCGT TGACCGCATG GCAGCTCCGA GAGCAGAGGC TCTATTTGAC
TTCACTGGAA ACAGCAAACT GGAGCTGAAT TTCAAAGCTG GAGATGTGAT CTTCCTCCTC
AGTCGGATCA ACAAAGACTG GCTGGAGGGC ACTGTCCGGG GAGCCACGGG CATCTTCCCT
CTCTCCTTCG TGAAGATCCT CAAAGACTTC CCTGAGGAGG ACGACCCCAC CAACTGGCTG
CGTTGCTACT ACTACGAAGA CACCATCAGC ACCATCAAGT CTGTGGCCTG GGAGGGAGGG
GCCTGTCCAG CCTTCCTGCC ATCCCTACGA CCACTGCCCC TCACATCACC TTCTCATGGG
TCCCTCTCCC ACTCCAAAGC CCCCAGTGGC TCCCAGATGA GCCACAATGC TGTAACAAGC
CATCAACGTC CAGGGTGGCC TGGCCAGCCT CATTCCCCTT TCCCCCACCC CACACCCCAC
TTCCAGCCTG ATGCCTCCTT ACTCCAGCCT GTCACCCCCT TAGGGACATC GCGGTGGAGG
AAGATCTCAG CAGCACTCCC CTATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16465
Clone ID Related Accession (Same CDS sequence) NM_000631
Accession Version NM_000631.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neutrophil cytosol factor 4 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC002798.1, BX355636.2 and BU076892.1. On Dec 20, 2007 this sequence version replaced gi:47519797. Transcript Variant: This variant (1) lacks an internal coding segment compared to variant 2, which leads to a translation frameshift. The resulting isoform (1) has a distinct and shorter C-terminus, as compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK223324.1, CR456528.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGTGG CCCAGCAGCT GCGGGCCGAG AGTGACTTTG AACAGCTTCC GGATGATGTT 
GCCATCTCGG CCAACATTGC TGACATCGAG GAGAAGAGAG GCTTCACCAG CCACTTTGTT
TTCGTCATCG AGGTGAAGAC AAAAGGAGGA TCCAAGTACC TCATCTACCG CCGCTACCGC
CAGTTCCATG CTTTGCAGAG CAAGCTGGAG GAGCGCTTCG GGCCAGACAG CAAGAGCAGT
GCCCTGGCCT GTACCCTGCC CACACTCCCA GCCAAAGTCT ACGTGGGTGT GAAACAGGAG
ATCGCCGAGA TGCGGATACC TGCCCTCAAC GCCTACATGA AGAGCCTGCT CAGCCTGCCG
GTCTGGGTGC TGATGGATGA GGACGTCCGG ATCTTCTTTT ACCAGTCGCC CTATGACTCA
GAGCAGGTGC CCCAGGCACT CCGCCGGCTC CGCCCGCGCA CCCGGAAAGT CAAGAGCGTG
TCCCCACAGG GCAACAGCGT TGACCGCATG GCAGCTCCGA GAGCAGAGGC TCTATTTGAC
TTCACTGGAA ACAGCAAACT GGAGCTGAAT TTCAAAGCTG GAGATGTGAT CTTCCTCCTC
AGTCGGATCA ACAAAGACTG GCTGGAGGGC ACTGTCCGGG GAGCCACGGG CATCTTCCCT
CTCTCCTTCG TGAAGATCCT CAAAGACTTC CCTGAGGAGG ACGACCCCAC CAACTGGCTG
CGTTGCTACT ACTACGAAGA CACCATCAGC ACCATCAAGG ACATCGCGGT GGAGGAAGAT
CTCAGCAGCA CTCCCCTATT GAAAGACCTG CTGGAGCTCA CAAGGCGGGA GTTCCAGAGA
GAGGACATAG CTCTGAATTA CCGGGACGCT GAGGGGGATC TGGTTCGGCT GCTGTCGGAT
GAGGACGTAG CGCTCATGGT GCGGCAGGCT CGTGGCCTCC CCTCCCAGAA GCGCCTCTTC
CCCTGGAAGC TGCACATCAC GCAGAAGGAC AACTACAGGG TCTACAACAC GATGCCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000622.2
CDS185..1204
Misc Feature(1)122..124(+)
Misc Feature(2)239..607(+)
Misc Feature(3)281..607(+)
Misc Feature(4)356..499(+)
Misc Feature(5)356..364(+)
Misc Feature(6)458..466(+)
Misc Feature(7)644..646(+)
Misc Feature(8)644..646(+)
Misc Feature(9)704..865(+)
Misc Feature(10)719..853(+)
Misc Feature(11)782..832(+)
Misc Feature(12)896..1171(+)
Misc Feature(13)953..1150(+)
Misc Feature(14)1049..1090(+)
Misc Feature(15)1127..1129(+)
Exon (1)1..216
Gene:NCF4
Gene Synonym:
Exon (2)217..301
Gene:NCF4
Gene Synonym:
Exon (3)302..455
Gene:NCF4
Gene Synonym:
Exon (4)456..526
Gene:NCF4
Gene Synonym:
Exon (5)527..654
Gene:NCF4
Gene Synonym:
Exon (6)655..712
Gene:NCF4
Gene Synonym:
Exon (7)713..811
Gene:NCF4
Gene Synonym:
Exon (8)812..942
Gene:NCF4
Gene Synonym:
Exon (9)943..1008
Gene:NCF4
Gene Synonym:
Exon (10)1009..1398
Gene:NCF4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000631
Organism Homo sapiens (human)
Definition Homo sapiens neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000631

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGTGG CCCAGCAGCT GCGGGCCGAG AGTGACTTTG AACAGCTTCC GGATGATGTT 
GCCATCTCGG CCAACATTGC TGACATCGAG GAGAAGAGAG GCTTCACCAG CCACTTTGTT
TTCGTCATCG AGGTGAAGAC AAAAGGAGGA TCCAAGTACC TCATCTACCG CCGCTACCGC
CAGTTCCATG CTTTGCAGAG CAAGCTGGAG GAGCGCTTCG GGCCAGACAG CAAGAGCAGT
GCCCTGGCCT GTACCCTGCC CACACTCCCA GCCAAAGTCT ACGTGGGTGT GAAACAGGAG
ATCGCCGAGA TGCGGATACC TGCCCTCAAC GCCTACATGA AGAGCCTGCT CAGCCTGCCG
GTCTGGGTGC TGATGGATGA GGACGTCCGG ATCTTCTTTT ACCAGTCGCC CTATGACTCA
GAGCAGGTGC CCCAGGCACT CCGCCGGCTC CGCCCGCGCA CCCGGAAAGT CAAGAGCGTG
TCCCCACAGG GCAACAGCGT TGACCGCATG GCAGCTCCGA GAGCAGAGGC TCTATTTGAC
TTCACTGGAA ACAGCAAACT GGAGCTGAAT TTCAAAGCTG GAGATGTGAT CTTCCTCCTC
AGTCGGATCA ACAAAGACTG GCTGGAGGGC ACTGTCCGGG GAGCCACGGG CATCTTCCCT
CTCTCCTTCG TGAAGATCCT CAAAGACTTC CCTGAGGAGG ACGACCCCAC CAACTGGCTG
CGTTGCTACT ACTACGAAGA CACCATCAGC ACCATCAAGG ACATCGCGGT GGAGGAAGAT
CTCAGCAGCA CTCCCCTATT GAAAGACCTG CTGGAGCTCA CAAGGCGGGA GTTCCAGAGA
GAGGACATAG CTCTGAATTA CCGGGACGCT GAGGGGGATC TGGTTCGGCT GCTGTCGGAT
GAGGACGTAG CGCTCATGGT GCGGCAGGCT CGTGGCCTCC CCTCCCAGAA GCGCCTCTTC
CCCTGGAAGC TGCACATCAC GCAGAAGGAC AACTACAGGG TCTACAACAC GATGCCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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