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NCF4 neutrophil cytosolic factor 4, 40kDa [Homo sapiens (human)]

Gene Symbol NCF4
Entrez Gene ID 4689
Full Name neutrophil cytosolic factor 4, 40kDa
Synonyms NCF, P40PHOX, SH3PXD4
General protein information
Preferred Names
neutrophil cytosol factor 4
Names
neutrophil cytosol factor 4
NCF-4
p40-phox
neutrophil NADPH oxidase factor 4
SH3 and PX domain-containing protein 4
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

22

22q13.1

Summary The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

601488

Disorder Html:





The following NCF4 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCF4 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu59223 XM_011530198 PREDICTED: Homo sapiens neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu59224 XM_011530199 PREDICTED: Homo sapiens neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu18835 NM_013416 Homo sapiens neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $379
OHu16465 NM_000631 Homo sapiens neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu59223
Accession Version XM_011530198.1
Sequence Information ORF Nucleotide Sequence (Length: 1194bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neutrophil cytosol factor 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGGGGCTC CCTGTACCTC GAAGGTCTTA TCTGAAAGCG AGCCTGGATT GTTCTGCTTA 
AGGTCAAAGC AGAGAGTACA AGGAGAGGGC GCAGGTTCGC GCAGCGGGGA CTTTCTAGAA
CCCCAAGACG ACGGTAGTGG CCGTGTATTG AGCAGTACTG AGCACCTACT GTATGCCGGG
CTCTGTTCTG GGCACTGGGA TGTAGGTGAC TTTGAACAGC TTCCGGATGA TGTTGCCATC
TCGGCCAACA TTGCTGACAT CGAGGAGAAG AGAGGCTTCA CCAGCCACTT TGTTTTCGTC
ATCGAGGTGA AGACAAAAGG AGGATCCAAG TACCTCATCT ACCGCCGCTA CCGCCAGTTC
CATGCTTTGC AGAGCAAGCT GGAGGAGCGC TTCGGGCCAG ACAGCAAGAG CAGTGCCCTG
GCCTGTACCC TGCCCACACT CCCAGCCAAA GTCTACGTGG GTGTGAAACA GGAGATCGCC
GAGATGCGGA TACCTGCCCT CAACGCCTAC ATGAAGAGCC TGCTCAGCCT GCCGGTCTGG
GTGCTGATGG ATGAGGACGT CCGGATCTTC TTTTACCAGT CGCCCTATGA CTCAGAGCAG
GTGCCCCAGG CACTCCGCCG GCTCCGCCCG CGCACCCGGA AAGTCAAGAG CGTGTCCCCA
CAGGGCAACA GCGTTGACCG CATGGCAGCT CCGAGAGCAG AGGCTCTATT TGACTTCACT
GGAAACAGCA AACTGGAGCT GAATTTCAAA GCTGGAGATG TGATCTTCCT CCTCAGTCGG
ATCAACAAAG ACTGGCTGGA GGGCACTGTC CGGGGAGCCA CGGGCATCTT CCCTCTCTCC
TTCGTGAAGA TCCTCAAAGA CTTCCCTGAG GAGGACGACC CCACCAACTG GCTGCGTTGC
TACTACTACG AAGACACCAT CAGCACCATC AAGGACATCG CGGTGGAGGA AGATCTCAGC
AGCACTCCCC TATTGAAAGA CCTGCTGGAG CTCACAAGGC GGGAGTTCCA GAGAGAGGAC
ATAGCTCTGA ATTACCGGGA CGCTGAGGGG GATCTGGTTC GGCTGCTGTC GGATGAGGAC
GTAGCGCTCA TGGTGCGGCA GGCTCGTGGC CTCCCCTCCC AGAAGCGCCT CTTCCCCTGG
AAGCTGCACA TCACGCAGAA GGACAACTAC AGGGTCTACA ACACGATGCC ATGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011528500.1
CDS793..1986
Misc Feature(1)1021..1389(+)
Misc Feature(2)1063..1389(+)
Misc Feature(3)1138..1281(+)
Misc Feature(4)1486..1647(+)
Misc Feature(5)1501..1635(+)
Misc Feature(6)1564..1614(+)
Misc Feature(7)1678..1953(+)
Misc Feature(8)1735..1932(+)
Misc Feature(9)1831..1872(+)
Translation
Position Chain Variation Link
3 3 c, t dbSNP:373208130
29 29 a, c dbSNP:36118061
46 46 a, g dbSNP:750507350
48 48 c, t dbSNP:377308943
60 60 c, t dbSNP:771599879
103 103 c, t dbSNP:184830500
115 115 a, g dbSNP:568695523
117 117 a, g dbSNP:138514693
142 142 a, t dbSNP:547788806
151 151 -, act dbSNP:201889273
165 165 a, g dbSNP:566432947
179 179 c, t dbSNP:758566653
192 192 a, c dbSNP:533554978
201 201 a, g dbSNP:558581188
231 231 a, c dbSNP:543307317
232 232 a, g dbSNP:570202557
252 252 a, g dbSNP:537596490
257 257 c, t dbSNP:189246938
258 258 a, g dbSNP:369404864
262 262 c, g dbSNP:555918097
272 272 c, t dbSNP:141562746
293 293 c, t dbSNP:752516830
317 317 c, t dbSNP:532243217
330 330 c, t dbSNP:34829508
348 348 g, t dbSNP:150795213
355 355 a, g dbSNP:572360045
363 363 a, g dbSNP:34661090
378 378 c, g dbSNP:749262980
384 384 a, g dbSNP:139224598
388 388 c, g dbSNP:180931634
390 390 a, g dbSNP:543965165
404 404 a, g dbSNP:35114095
405 405 c, t dbSNP:35301252
434 434 a, c dbSNP:373593526
437 437 c, t dbSNP:140519510
455 455 c, t dbSNP:771654245
469 469 a, g dbSNP:775160773
471 471 c, t dbSNP:34730019
477 477 c, g dbSNP:533720652
480 480 a, t dbSNP:373336224
509 509 c, t dbSNP:145418415
510 510 a, g dbSNP:570142482
514 514 c, t dbSNP:537533389
515 515 a, g dbSNP:769185214
519 519 -, ggt dbSNP:199987688
537 537 c, t dbSNP:555856713
538 538 a, g dbSNP:777134458
539 539 a, g dbSNP:147700493
584 584 a, g dbSNP:535295897
587 587 c, t dbSNP:553996376
595 595 a, c dbSNP:762470401
596 596 a, g dbSNP:572244234
599 599 c, t dbSNP:4821544
602 602 c, t dbSNP:558166038
604 604 c, t dbSNP:576518323
617 617 c, t dbSNP:142335119
649 649 g, t dbSNP:571260355
654 654 a, g dbSNP:78863304
672 672 c, t dbSNP:562165010
673 673 g, t dbSNP:574135245
691 691 a, t dbSNP:184934107
707 707 c, t dbSNP:537084152
720 720 a, c dbSNP:766528193
726 726 c, t dbSNP:773014764
730 730 g, t dbSNP:751825814
747 747 c, g dbSNP:9619637
759 759 c, t dbSNP:746934
791 791 a, g dbSNP:537281735
793 793 a, g dbSNP:142296093
797 797 a, g dbSNP:151326045
817 817 g, t dbSNP:563705444
824 824 c, g dbSNP:376233375
831 831 c, t dbSNP:753617523
832 832 a, g dbSNP:531229397
872 872 a, g dbSNP:575213771
882 882 c, t dbSNP:568074877
890 890 c, t dbSNP:377751377
897 897 c, t dbSNP:189874596
898 898 a, g dbSNP:547274295
909 909 a, g dbSNP:741997
920 920 a, g dbSNP:374570915
921 921 c, t dbSNP:778089996
934 934 c, t dbSNP:758265744
941 941 g, t dbSNP:555474408
962 962 c, t dbSNP:367751200
969 969 c, t dbSNP:534768459
979 979 a, t dbSNP:371228405
1002 1002 c, g dbSNP:766618219
1004 1004 c, t dbSNP:754276557
1012 1012 c, t dbSNP:755356622
1016 1016 c, t dbSNP:147322774
1017 1017 a, g dbSNP:139289225
1017 1017 -, g dbSNP:746841045
1024 1024 a, g dbSNP:575470394
1029 1029 c, t dbSNP:34373276
1035 1035 a, g dbSNP:10854695
1040 1040 a, g dbSNP:573525247
1042 1042 a, g dbSNP:541007080
1044 1044 c, t dbSNP:139319252
1052 1052 a, c, t dbSNP:13057803
1053 1053 c, g, t dbSNP:149998137
1054 1054 -, gag dbSNP:770609723
1054 1054 a, g dbSNP:775229266
1056 1056 a, g dbSNP:200822582
1063 1063 a, g dbSNP:768042374
1064 1064 g, t dbSNP:773949075
1065 1065 -, aggct dbSNP:776368871
1065 1065 a, g dbSNP:761061449
1067 1067 a, c, g, t dbSNP:766859628
1068 1068 a, c, t dbSNP:765598164
1069 1069 c, t dbSNP:758660640
1070 1070 a, c, t dbSNP:764060580
1071 1071 c, t dbSNP:757314998
1072 1072 -, ctccc dbSNP:745470237
1072 1072 a, c dbSNP:200818199
1075 1075 a, g dbSNP:199943692
1089 1089 c, t dbSNP:369350039
1090 1090 a, c, g dbSNP:371578849
1093 1093 a, g dbSNP:779970175
1095 1095 c, t dbSNP:753635134
1098 1098 c, g dbSNP:754759226
1108 1108 -, aaggaggatc dbSNP:762209908
1111 1111 a, g dbSNP:148715332
1123 1123 c, t dbSNP:747769633
1128 1128 a, c dbSNP:771597330
1133 1133 -, cccc dbSNP:144929792
1135 1135 c, t dbSNP:767200492
1136 1136 g, t dbSNP:746353194
1137 1137 a, c dbSNP:770239095
1138 1138 c, t dbSNP:143532979
1139 1139 a, g dbSNP:763314327
1144 1144 c, t dbSNP:183179978
1145 1145 a, g dbSNP:369847561
1146 1146 c, t dbSNP:762003847
1154 1154 a, g dbSNP:767668921
1167 1167 c, g dbSNP:750743658
1180 1180 c, t dbSNP:750577331
1185 1185 c, t dbSNP:373041130
1186 1186 a, g dbSNP:766377093
1188 1188 a, g, t dbSNP:377510654
1191 1191 a, g dbSNP:778630595
1194 1194 c, g, t dbSNP:188209920
1195 1195 a, g dbSNP:777426455
1204 1204 a, g dbSNP:373447489
1206 1206 c, t dbSNP:35431748
1208 1208 c, t dbSNP:780484949
1219 1219 a, g dbSNP:749822456
1220 1220 a, c dbSNP:112306225
1224 1224 a, g dbSNP:774613957
1233 1233 a, c dbSNP:748376366
1236 1236 a, t dbSNP:370754874
1237 1237 g, t dbSNP:773596460
1244 1244 c, t dbSNP:747292434
1248 1248 c, t dbSNP:771129807
1249 1249 a, g dbSNP:112273712
1264 1264 g, t dbSNP:759685640
1266 1266 a, g dbSNP:765046425
1267 1267 a, g dbSNP:775504785
1269 1269 c, t dbSNP:746977479
1270 1270 a, g dbSNP:763938344
1272 1272 c, t dbSNP:34072404
1279 1279 c, t dbSNP:756948322
1280 1280 a, c, g, t dbSNP:387906808
1281 1281 c, g dbSNP:779218017
1283 1283 c, t dbSNP:748676281
1296 1296 c, t dbSNP:373828331
1297 1297 a, g dbSNP:778347689
1300 1300 c, t dbSNP:747202725
1304 1304 g, t dbSNP:576610489
1310 1310 a, g dbSNP:567656365
1311 1311 c, t dbSNP:774300357
1314 1314 a, g dbSNP:761526575
1319 1319 a, g dbSNP:9610595
1321 1321 c, t dbSNP:377706087
1324 1324 c, g dbSNP:772724160
1325 1325 a, c, t dbSNP:760441596
1326 1326 a, g dbSNP:753447204
1327 1327 a, g, t dbSNP:759148798
1329 1329 c, t dbSNP:752127204
1338 1338 a, g, t dbSNP:368863802
1350 1350 c, t dbSNP:144105468
1355 1355 c, g dbSNP:750583738
1356 1356 a, g dbSNP:199642717
1372 1372 c, t dbSNP:780280945
1373 1373 c, t dbSNP:749322339
1374 1374 a, g dbSNP:755052012
1376 1376 c, t dbSNP:778761203
1387 1387 g, t dbSNP:748172203
1395 1395 a, g dbSNP:771758194
1401 1401 g, t dbSNP:773098220
1407 1407 c, g dbSNP:746619780
1408 1408 c, t dbSNP:770577417
1409 1409 a, g dbSNP:376709783
1411 1411 a, c dbSNP:759057034
1412 1412 a, g dbSNP:371098446
1413 1413 -, gctccgccc dbSNP:754040377
1413 1413 a, g, t dbSNP:764896112
1417 1417 c, t dbSNP:150976323
1418 1418 a, g dbSNP:767747416
1419 1419 c, t dbSNP:750746060
1422 1422 a, g, t dbSNP:554368196
1423 1423 c, t dbSNP:572795915
1424 1424 a, g dbSNP:35160112
1425 1425 c, t dbSNP:779032981
1429 1429 a, c dbSNP:758154907
1443 1443 c, t dbSNP:752773971
1444 1444 a, c, g dbSNP:150103256
1448 1448 c, t dbSNP:751507562
1463 1463 g, t dbSNP:757112377
1464 1464 c, t dbSNP:560584199
1465 1465 a, g dbSNP:745490664
1472 1472 a, g dbSNP:371308317
1485 1485 a, g dbSNP:779600542
1492 1492 a, g dbSNP:748770662
1498 1498 c, t dbSNP:767768687
1502 1502 c, t dbSNP:773281682
1515 1515 a, g dbSNP:760573346
1519 1519 a, g dbSNP:766040731
1526 1526 c, t dbSNP:766177098
1552 1552 g, t dbSNP:753529855
1553 1553 g, t dbSNP:754833887
1556 1556 a, t dbSNP:764803761
1563 1563 c, t dbSNP:752404521
1564 1564 c, t dbSNP:559758444
1570 1570 a, c, t dbSNP:138411102
1571 1571 a, c, g dbSNP:35355915
1572 1572 a, g dbSNP:749545374
1574 1574 a, t dbSNP:769074205
1579 1579 -, aa dbSNP:757106743
1583 1583 a, g dbSNP:774706034
1587 1587 g, t dbSNP:748453334
1589 1589 g, t dbSNP:772249899
1590 1590 a, g dbSNP:773477832
1592 1592 a, c dbSNP:760832946
1604 1604 a, g dbSNP:376569226
1613 1613 c, t dbSNP:146911421
1614 1614 a, g dbSNP:190625962
1621 1621 c, t dbSNP:761212596
1625 1625 -, ct dbSNP:780782557
1625 1625 c, t dbSNP:767125572
1631 1631 c, t dbSNP:754272966
1635 1635 c, t dbSNP:755594287
1636 1636 a, g dbSNP:200068754
1641 1641 c, g dbSNP:753071695
1642 1642 a, c dbSNP:368429862
1650 1650 a, c dbSNP:777800037
1652 1652 a, t dbSNP:747315841
1654 1654 g, t dbSNP:770883606
1656 1656 c, t dbSNP:781381492
1668 1668 c, t dbSNP:745954906
1670 1670 -, a dbSNP:745545652
1671 1671 c, t dbSNP:150656826
1673 1673 c, t dbSNP:775408197
1683 1683 g, t dbSNP:762676581
1687 1687 c, t dbSNP:768494262
1688 1688 a, g dbSNP:575016108
1695 1695 c, t dbSNP:761570088
1701 1701 c, g, t dbSNP:2072712
1702 1702 a, g dbSNP:560705177
1704 1704 a, g dbSNP:765774359
1708 1708 a, g dbSNP:753271883
1709 1709 c, t dbSNP:548576102
1713 1713 a, c dbSNP:147089818
1715 1715 a, g dbSNP:546475819
1716 1716 c, t dbSNP:757450151
1718 1718 c, t dbSNP:781298815
1729 1729 a, g dbSNP:772400898
1732 1732 a, g dbSNP:773520052
1733 1733 c, t dbSNP:761060692
1734 1734 a, g dbSNP:766525712
1737 1737 a, g dbSNP:754075839
1746 1746 g, t dbSNP:759649020
1762 1762 c, t dbSNP:374646787
1764 1764 a, g dbSNP:752651381
1765 1765 c, t dbSNP:139432640
1767 1767 c, g dbSNP:777882570
1775 1775 g, t dbSNP:751332212
1779 1779 a, g dbSNP:144124883
1782 1782 a, g dbSNP:780975917
1791 1791 g, t dbSNP:145005349
1792 1792 c, t dbSNP:200598824
1793 1793 a, g dbSNP:769839943
1805 1805 g, t dbSNP:775794915
1809 1809 a, g dbSNP:763111423
1811 1811 a, g dbSNP:764332022
1813 1813 a, g dbSNP:774395484
1815 1815 a, t dbSNP:761887926
1828 1828 c, g, t dbSNP:148661727
1829 1829 a, g dbSNP:750147229
1831 1831 a, g dbSNP:755943893
1833 1833 c, t dbSNP:765893996
1834 1834 a, g dbSNP:753588526
1838 1838 a, t dbSNP:754480497
1840 1840 a, g dbSNP:778435771
1843 1843 a, g dbSNP:752040969
1845 1845 a, c, t dbSNP:201317613
1846 1846 c, g dbSNP:781723897
1852 1852 c, g dbSNP:199500127
1853 1853 a, g, t dbSNP:554387451
1863 1863 a, g dbSNP:11552115
1872 1872 c, t dbSNP:567611718
1873 1873 a, g, t dbSNP:201552121
1874 1874 g, t dbSNP:762012398
1877 1877 a, c, t dbSNP:5995361
1878 1878 a, g dbSNP:200914046
1879 1879 c, t dbSNP:766267228
1888 1888 c, t dbSNP:546311292
1889 1889 a, g dbSNP:141160114
1895 1895 c, g dbSNP:764893385
1896 1896 c, t dbSNP:752165248
1897 1897 a, c, t dbSNP:757937260
1898 1898 a, g dbSNP:751005224
1900 1900 g, t dbSNP:199618052
1906 1906 a, c dbSNP:199686562
1907 1907 c, t dbSNP:200966942
1918 1918 c, t dbSNP:780513211
1919 1919 a, g, t dbSNP:371008750
1923 1923 c, t dbSNP:779285588
1926 1926 c, t dbSNP:1858
1932 1932 a, g dbSNP:772255576
1944 1944 a, c dbSNP:370312224
1946 1946 c, t dbSNP:367876433
1947 1947 a, g dbSNP:376148807
1973 1973 a, g dbSNP:776362467
1974 1974 c, t dbSNP:759359854
1976 1976 c, t dbSNP:147550660
1977 1977 a, g dbSNP:28669668
1979 1979 g, t dbSNP:762365393
1980 1980 -, gcc dbSNP:775117377
1992 1992 c, t dbSNP:763700539
1993 1993 a, g dbSNP:750868959
1997 1997 -, c dbSNP:36023462
1999 1999 c, t dbSNP:756592059
2003 2003 c, g dbSNP:767020866
2005 2005 a, g dbSNP:754185606
2009 2009 c, t dbSNP:755408986
2016 2016 a, t dbSNP:557549740
2034 2034 a, g dbSNP:748451564
2049 2049 c, t dbSNP:781057169
2064 2064 c, g dbSNP:576231383
2066 2066 -, g dbSNP:34155264
2082 2082 c, g dbSNP:182197208
2087 2087 c, t dbSNP:541831946
2091 2091 c, t dbSNP:748052138
2108 2108 c, t dbSNP:186232557
2109 2109 a, g dbSNP:562129519
2119 2119 a, t dbSNP:561904370
2158 2158 a, c dbSNP:529161346
2168 2168 a, t dbSNP:540990854

Target ORF information:

RefSeq Version XM_011530198
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011530198

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGGGGCTC CCTGTACCTC GAAGGTCTTA TCTGAAAGCG AGCCTGGATT GTTCTGCTTA 
AGGTCAAAGC AGAGAGTACA AGGAGAGGGC GCAGGTTCGC GCAGCGGGGA CTTTCTAGAA
CCCCAAGACG ACGGTAGTGG CCGTGTATTG AGCAGTACTG AGCACCTACT GTATGCCGGG
CTCTGTTCTG GGCACTGGGA TGTAGGTGAC TTTGAACAGC TTCCGGATGA TGTTGCCATC
TCGGCCAACA TTGCTGACAT CGAGGAGAAG AGAGGCTTCA CCAGCCACTT TGTTTTCGTC
ATCGAGGTGA AGACAAAAGG AGGATCCAAG TACCTCATCT ACCGCCGCTA CCGCCAGTTC
CATGCTTTGC AGAGCAAGCT GGAGGAGCGC TTCGGGCCAG ACAGCAAGAG CAGTGCCCTG
GCCTGTACCC TGCCCACACT CCCAGCCAAA GTCTACGTGG GTGTGAAACA GGAGATCGCC
GAGATGCGGA TACCTGCCCT CAACGCCTAC ATGAAGAGCC TGCTCAGCCT GCCGGTCTGG
GTGCTGATGG ATGAGGACGT CCGGATCTTC TTTTACCAGT CGCCCTATGA CTCAGAGCAG
GTGCCCCAGG CACTCCGCCG GCTCCGCCCG CGCACCCGGA AAGTCAAGAG CGTGTCCCCA
CAGGGCAACA GCGTTGACCG CATGGCAGCT CCGAGAGCAG AGGCTCTATT TGACTTCACT
GGAAACAGCA AACTGGAGCT GAATTTCAAA GCTGGAGATG TGATCTTCCT CCTCAGTCGG
ATCAACAAAG ACTGGCTGGA GGGCACTGTC CGGGGAGCCA CGGGCATCTT CCCTCTCTCC
TTCGTGAAGA TCCTCAAAGA CTTCCCTGAG GAGGACGACC CCACCAACTG GCTGCGTTGC
TACTACTACG AAGACACCAT CAGCACCATC AAGGACATCG CGGTGGAGGA AGATCTCAGC
AGCACTCCCC TATTGAAAGA CCTGCTGGAG CTCACAAGGC GGGAGTTCCA GAGAGAGGAC
ATAGCTCTGA ATTACCGGGA CGCTGAGGGG GATCTGGTTC GGCTGCTGTC GGATGAGGAC
GTAGCGCTCA TGGTGCGGCA GGCTCGTGGC CTCCCCTCCC AGAAGCGCCT CTTCCCCTGG
AAGCTGCACA TCACGCAGAA GGACAACTAC AGGGTCTACA ACACGATGCC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59224
Accession Version XM_011530199.1
Sequence Information ORF Nucleotide Sequence (Length: 1164bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neutrophil cytosol factor 4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCTCATTT ATTATTTTTT CTTGAACGTT GGCTCCCCCA TGAGAAGCAA GCTCCACGAG 
GCTGTGTCCG TTTTGATCGC TGCTGTATCA CTAGGAGACG ACGGTAGTGG CCGTGTATTG
AGCAGTACTG AGCACCTACT GTATGCCGGG CTCTGTTCTG GGCACTGGGA TGTAGGTGAC
TTTGAACAGC TTCCGGATGA TGTTGCCATC TCGGCCAACA TTGCTGACAT CGAGGAGAAG
AGAGGCTTCA CCAGCCACTT TGTTTTCGTC ATCGAGGTGA AGACAAAAGG AGGATCCAAG
TACCTCATCT ACCGCCGCTA CCGCCAGTTC CATGCTTTGC AGAGCAAGCT GGAGGAGCGC
TTCGGGCCAG ACAGCAAGAG CAGTGCCCTG GCCTGTACCC TGCCCACACT CCCAGCCAAA
GTCTACGTGG GTGTGAAACA GGAGATCGCC GAGATGCGGA TACCTGCCCT CAACGCCTAC
ATGAAGAGCC TGCTCAGCCT GCCGGTCTGG GTGCTGATGG ATGAGGACGT CCGGATCTTC
TTTTACCAGT CGCCCTATGA CTCAGAGCAG GTGCCCCAGG CACTCCGCCG GCTCCGCCCG
CGCACCCGGA AAGTCAAGAG CGTGTCCCCA CAGGGCAACA GCGTTGACCG CATGGCAGCT
CCGAGAGCAG AGGCTCTATT TGACTTCACT GGAAACAGCA AACTGGAGCT GAATTTCAAA
GCTGGAGATG TGATCTTCCT CCTCAGTCGG ATCAACAAAG ACTGGCTGGA GGGCACTGTC
CGGGGAGCCA CGGGCATCTT CCCTCTCTCC TTCGTGAAGA TCCTCAAAGA CTTCCCTGAG
GAGGACGACC CCACCAACTG GCTGCGTTGC TACTACTACG AAGACACCAT CAGCACCATC
AAGGACATCG CGGTGGAGGA AGATCTCAGC AGCACTCCCC TATTGAAAGA CCTGCTGGAG
CTCACAAGGC GGGAGTTCCA GAGAGAGGAC ATAGCTCTGA ATTACCGGGA CGCTGAGGGG
GATCTGGTTC GGCTGCTGTC GGATGAGGAC GTAGCGCTCA TGGTGCGGCA GGCTCGTGGC
CTCCCCTCCC AGAAGCGCCT CTTCCCCTGG AAGCTGCACA TCACGCAGAA GGACAACTAC
AGGGTCTACA ACACGATGCC ATGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011528501.1
CDS489..1652
Misc Feature(1)687..1055(+)
Misc Feature(2)729..1055(+)
Misc Feature(3)804..947(+)
Misc Feature(4)1152..1313(+)
Misc Feature(5)1167..1301(+)
Misc Feature(6)1230..1280(+)
Misc Feature(7)1344..1619(+)
Misc Feature(8)1401..1598(+)
Misc Feature(9)1497..1538(+)
Translation
Position Chain Variation Link
4 4 c, g dbSNP:769723907
23 23 g, t dbSNP:772170450
26 26 c, t dbSNP:560332016
30 30 a, g dbSNP:535632871
46 46 c, t dbSNP:773088620
54 54 c, g dbSNP:527765264
56 56 c, t dbSNP:368167939
66 66 c, t dbSNP:570993177
134 134 a, c dbSNP:538465190
185 185 c, g dbSNP:550452737
211 211 c, t dbSNP:748816364
212 212 a, g dbSNP:568782754
230 230 c, t dbSNP:770474172
231 231 a, g dbSNP:112355982
243 243 a, t dbSNP:556936293
252 252 a, c dbSNP:555929789
264 264 a, g dbSNP:112974908
323 323 a, g dbSNP:142415082
356 356 c, g dbSNP:755052622
399 399 c, t dbSNP:572782088
400 400 a, c dbSNP:759177148
401 401 c, t dbSNP:372077923
440 440 a, c dbSNP:34421414
457 457 g, t dbSNP:190176892
476 476 g, t dbSNP:145974544
504 504 a, t dbSNP:181817524
517 517 c, t dbSNP:555981099
545 545 c, t dbSNP:368988350
549 549 g, t dbSNP:372253348
550 550 c, t dbSNP:11912791
558 558 a, g dbSNP:527701548
566 566 c, t dbSNP:564122804
573 573 a, g dbSNP:758685601
583 583 a, g dbSNP:2413426
586 586 a, g dbSNP:374570915
587 587 c, t dbSNP:778089996
600 600 c, t dbSNP:758265744
607 607 g, t dbSNP:555474408
628 628 c, t dbSNP:367751200
635 635 c, t dbSNP:534768459
645 645 a, t dbSNP:371228405
668 668 c, g dbSNP:766618219
670 670 c, t dbSNP:754276557
678 678 c, t dbSNP:755356622
682 682 c, t dbSNP:147322774
683 683 a, g dbSNP:139289225
683 683 -, g dbSNP:746841045
690 690 a, g dbSNP:575470394
695 695 c, t dbSNP:34373276
701 701 a, g dbSNP:10854695
706 706 a, g dbSNP:573525247
708 708 a, g dbSNP:541007080
710 710 c, t dbSNP:139319252
718 718 a, c, t dbSNP:13057803
719 719 c, g, t dbSNP:149998137
720 720 -, gag dbSNP:770609723
720 720 a, g dbSNP:775229266
722 722 a, g dbSNP:200822582
729 729 a, g dbSNP:768042374
730 730 g, t dbSNP:773949075
731 731 -, aggct dbSNP:776368871
731 731 a, g dbSNP:761061449
733 733 a, c, g, t dbSNP:766859628
734 734 a, c, t dbSNP:765598164
735 735 c, t dbSNP:758660640
736 736 a, c, t dbSNP:764060580
737 737 c, t dbSNP:757314998
738 738 -, ctccc dbSNP:745470237
738 738 a, c dbSNP:200818199
741 741 a, g dbSNP:199943692
755 755 c, t dbSNP:369350039
756 756 a, c, g dbSNP:371578849
759 759 a, g dbSNP:779970175
761 761 c, t dbSNP:753635134
764 764 c, g dbSNP:754759226
774 774 -, aaggaggatc dbSNP:762209908
777 777 a, g dbSNP:148715332
789 789 c, t dbSNP:747769633
794 794 a, c dbSNP:771597330
799 799 -, cccc dbSNP:144929792
801 801 c, t dbSNP:767200492
802 802 g, t dbSNP:746353194
803 803 a, c dbSNP:770239095
804 804 c, t dbSNP:143532979
805 805 a, g dbSNP:763314327
810 810 c, t dbSNP:183179978
811 811 a, g dbSNP:369847561
812 812 c, t dbSNP:762003847
820 820 a, g dbSNP:767668921
833 833 c, g dbSNP:750743658
846 846 c, t dbSNP:750577331
851 851 c, t dbSNP:373041130
852 852 a, g dbSNP:766377093
854 854 a, g, t dbSNP:377510654
857 857 a, g dbSNP:778630595
860 860 c, g, t dbSNP:188209920
861 861 a, g dbSNP:777426455
870 870 a, g dbSNP:373447489
872 872 c, t dbSNP:35431748
874 874 c, t dbSNP:780484949
885 885 a, g dbSNP:749822456
886 886 a, c dbSNP:112306225
890 890 a, g dbSNP:774613957
899 899 a, c dbSNP:748376366
902 902 a, t dbSNP:370754874
903 903 g, t dbSNP:773596460
910 910 c, t dbSNP:747292434
914 914 c, t dbSNP:771129807
915 915 a, g dbSNP:112273712
930 930 g, t dbSNP:759685640
932 932 a, g dbSNP:765046425
933 933 a, g dbSNP:775504785
935 935 c, t dbSNP:746977479
936 936 a, g dbSNP:763938344
938 938 c, t dbSNP:34072404
945 945 c, t dbSNP:756948322
946 946 a, c, g, t dbSNP:387906808
947 947 c, g dbSNP:779218017
949 949 c, t dbSNP:748676281
962 962 c, t dbSNP:373828331
963 963 a, g dbSNP:778347689
966 966 c, t dbSNP:747202725
970 970 g, t dbSNP:576610489
976 976 a, g dbSNP:567656365
977 977 c, t dbSNP:774300357
980 980 a, g dbSNP:761526575
985 985 a, g dbSNP:9610595
987 987 c, t dbSNP:377706087
990 990 c, g dbSNP:772724160
991 991 a, c, t dbSNP:760441596
992 992 a, g dbSNP:753447204
993 993 a, g, t dbSNP:759148798
995 995 c, t dbSNP:752127204
1004 1004 a, g, t dbSNP:368863802
1016 1016 c, t dbSNP:144105468
1021 1021 c, g dbSNP:750583738
1022 1022 a, g dbSNP:199642717
1038 1038 c, t dbSNP:780280945
1039 1039 c, t dbSNP:749322339
1040 1040 a, g dbSNP:755052012
1042 1042 c, t dbSNP:778761203
1053 1053 g, t dbSNP:748172203
1061 1061 a, g dbSNP:771758194
1067 1067 g, t dbSNP:773098220
1073 1073 c, g dbSNP:746619780
1074 1074 c, t dbSNP:770577417
1075 1075 a, g dbSNP:376709783
1077 1077 a, c dbSNP:759057034
1078 1078 a, g dbSNP:371098446
1079 1079 -, gctccgccc dbSNP:754040377
1079 1079 a, g, t dbSNP:764896112
1083 1083 c, t dbSNP:150976323
1084 1084 a, g dbSNP:767747416
1085 1085 c, t dbSNP:750746060
1088 1088 a, g, t dbSNP:554368196
1089 1089 c, t dbSNP:572795915
1090 1090 a, g dbSNP:35160112
1091 1091 c, t dbSNP:779032981
1095 1095 a, c dbSNP:758154907
1109 1109 c, t dbSNP:752773971
1110 1110 a, c, g dbSNP:150103256
1114 1114 c, t dbSNP:751507562
1129 1129 g, t dbSNP:757112377
1130 1130 c, t dbSNP:560584199
1131 1131 a, g dbSNP:745490664
1138 1138 a, g dbSNP:371308317
1151 1151 a, g dbSNP:779600542
1158 1158 a, g dbSNP:748770662
1164 1164 c, t dbSNP:767768687
1168 1168 c, t dbSNP:773281682
1181 1181 a, g dbSNP:760573346
1185 1185 a, g dbSNP:766040731
1192 1192 c, t dbSNP:766177098
1218 1218 g, t dbSNP:753529855
1219 1219 g, t dbSNP:754833887
1222 1222 a, t dbSNP:764803761
1229 1229 c, t dbSNP:752404521
1230 1230 c, t dbSNP:559758444
1236 1236 a, c, t dbSNP:138411102
1237 1237 a, c, g dbSNP:35355915
1238 1238 a, g dbSNP:749545374
1240 1240 a, t dbSNP:769074205
1245 1245 -, aa dbSNP:757106743
1249 1249 a, g dbSNP:774706034
1253 1253 g, t dbSNP:748453334
1255 1255 g, t dbSNP:772249899
1256 1256 a, g dbSNP:773477832
1258 1258 a, c dbSNP:760832946
1270 1270 a, g dbSNP:376569226
1279 1279 c, t dbSNP:146911421
1280 1280 a, g dbSNP:190625962
1287 1287 c, t dbSNP:761212596
1291 1291 -, ct dbSNP:780782557
1291 1291 c, t dbSNP:767125572
1297 1297 c, t dbSNP:754272966
1301 1301 c, t dbSNP:755594287
1302 1302 a, g dbSNP:200068754
1307 1307 c, g dbSNP:753071695
1308 1308 a, c dbSNP:368429862
1316 1316 a, c dbSNP:777800037
1318 1318 a, t dbSNP:747315841
1320 1320 g, t dbSNP:770883606
1322 1322 c, t dbSNP:781381492
1334 1334 c, t dbSNP:745954906
1336 1336 -, a dbSNP:745545652
1337 1337 c, t dbSNP:150656826
1339 1339 c, t dbSNP:775408197
1349 1349 g, t dbSNP:762676581
1353 1353 c, t dbSNP:768494262
1354 1354 a, g dbSNP:575016108
1361 1361 c, t dbSNP:761570088
1367 1367 c, g, t dbSNP:2072712
1368 1368 a, g dbSNP:560705177
1370 1370 a, g dbSNP:765774359
1374 1374 a, g dbSNP:753271883
1375 1375 c, t dbSNP:548576102
1379 1379 a, c dbSNP:147089818
1381 1381 a, g dbSNP:546475819
1382 1382 c, t dbSNP:757450151
1384 1384 c, t dbSNP:781298815
1395 1395 a, g dbSNP:772400898
1398 1398 a, g dbSNP:773520052
1399 1399 c, t dbSNP:761060692
1400 1400 a, g dbSNP:766525712
1403 1403 a, g dbSNP:754075839
1412 1412 g, t dbSNP:759649020
1428 1428 c, t dbSNP:374646787
1430 1430 a, g dbSNP:752651381
1431 1431 c, t dbSNP:139432640
1433 1433 c, g dbSNP:777882570
1441 1441 g, t dbSNP:751332212
1445 1445 a, g dbSNP:144124883
1448 1448 a, g dbSNP:780975917
1457 1457 g, t dbSNP:145005349
1458 1458 c, t dbSNP:200598824
1459 1459 a, g dbSNP:769839943
1471 1471 g, t dbSNP:775794915
1475 1475 a, g dbSNP:763111423
1477 1477 a, g dbSNP:764332022
1479 1479 a, g dbSNP:774395484
1481 1481 a, t dbSNP:761887926
1494 1494 c, g, t dbSNP:148661727
1495 1495 a, g dbSNP:750147229
1497 1497 a, g dbSNP:755943893
1499 1499 c, t dbSNP:765893996
1500 1500 a, g dbSNP:753588526
1504 1504 a, t dbSNP:754480497
1506 1506 a, g dbSNP:778435771
1509 1509 a, g dbSNP:752040969
1511 1511 a, c, t dbSNP:201317613
1512 1512 c, g dbSNP:781723897
1518 1518 c, g dbSNP:199500127
1519 1519 a, g, t dbSNP:554387451
1529 1529 a, g dbSNP:11552115
1538 1538 c, t dbSNP:567611718
1539 1539 a, g, t dbSNP:201552121
1540 1540 g, t dbSNP:762012398
1543 1543 a, c, t dbSNP:5995361
1544 1544 a, g dbSNP:200914046
1545 1545 c, t dbSNP:766267228
1554 1554 c, t dbSNP:546311292
1555 1555 a, g dbSNP:141160114
1561 1561 c, g dbSNP:764893385
1562 1562 c, t dbSNP:752165248
1563 1563 a, c, t dbSNP:757937260
1564 1564 a, g dbSNP:751005224
1566 1566 g, t dbSNP:199618052
1572 1572 a, c dbSNP:199686562
1573 1573 c, t dbSNP:200966942
1584 1584 c, t dbSNP:780513211
1585 1585 a, g, t dbSNP:371008750
1589 1589 c, t dbSNP:779285588
1592 1592 c, t dbSNP:1858
1598 1598 a, g dbSNP:772255576
1610 1610 a, c dbSNP:370312224
1612 1612 c, t dbSNP:367876433
1613 1613 a, g dbSNP:376148807
1639 1639 a, g dbSNP:776362467
1640 1640 c, t dbSNP:759359854
1642 1642 c, t dbSNP:147550660
1643 1643 a, g dbSNP:28669668
1645 1645 g, t dbSNP:762365393
1646 1646 -, gcc dbSNP:775117377
1658 1658 c, t dbSNP:763700539
1659 1659 a, g dbSNP:750868959
1663 1663 -, c dbSNP:36023462
1665 1665 c, t dbSNP:756592059
1669 1669 c, g dbSNP:767020866
1671 1671 a, g dbSNP:754185606
1675 1675 c, t dbSNP:755408986
1682 1682 a, t dbSNP:557549740
1700 1700 a, g dbSNP:748451564
1715 1715 c, t dbSNP:781057169
1730 1730 c, g dbSNP:576231383
1732 1732 -, g dbSNP:34155264
1748 1748 c, g dbSNP:182197208
1753 1753 c, t dbSNP:541831946
1757 1757 c, t dbSNP:748052138
1774 1774 c, t dbSNP:186232557
1775 1775 a, g dbSNP:562129519
1785 1785 a, t dbSNP:561904370
1824 1824 a, c dbSNP:529161346
1834 1834 a, t dbSNP:540990854

Target ORF information:

RefSeq Version XM_011530199
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011530199

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCTCATTT ATTATTTTTT CTTGAACGTT GGCTCCCCCA TGAGAAGCAA GCTCCACGAG 
GCTGTGTCCG TTTTGATCGC TGCTGTATCA CTAGGAGACG ACGGTAGTGG CCGTGTATTG
AGCAGTACTG AGCACCTACT GTATGCCGGG CTCTGTTCTG GGCACTGGGA TGTAGGTGAC
TTTGAACAGC TTCCGGATGA TGTTGCCATC TCGGCCAACA TTGCTGACAT CGAGGAGAAG
AGAGGCTTCA CCAGCCACTT TGTTTTCGTC ATCGAGGTGA AGACAAAAGG AGGATCCAAG
TACCTCATCT ACCGCCGCTA CCGCCAGTTC CATGCTTTGC AGAGCAAGCT GGAGGAGCGC
TTCGGGCCAG ACAGCAAGAG CAGTGCCCTG GCCTGTACCC TGCCCACACT CCCAGCCAAA
GTCTACGTGG GTGTGAAACA GGAGATCGCC GAGATGCGGA TACCTGCCCT CAACGCCTAC
ATGAAGAGCC TGCTCAGCCT GCCGGTCTGG GTGCTGATGG ATGAGGACGT CCGGATCTTC
TTTTACCAGT CGCCCTATGA CTCAGAGCAG GTGCCCCAGG CACTCCGCCG GCTCCGCCCG
CGCACCCGGA AAGTCAAGAG CGTGTCCCCA CAGGGCAACA GCGTTGACCG CATGGCAGCT
CCGAGAGCAG AGGCTCTATT TGACTTCACT GGAAACAGCA AACTGGAGCT GAATTTCAAA
GCTGGAGATG TGATCTTCCT CCTCAGTCGG ATCAACAAAG ACTGGCTGGA GGGCACTGTC
CGGGGAGCCA CGGGCATCTT CCCTCTCTCC TTCGTGAAGA TCCTCAAAGA CTTCCCTGAG
GAGGACGACC CCACCAACTG GCTGCGTTGC TACTACTACG AAGACACCAT CAGCACCATC
AAGGACATCG CGGTGGAGGA AGATCTCAGC AGCACTCCCC TATTGAAAGA CCTGCTGGAG
CTCACAAGGC GGGAGTTCCA GAGAGAGGAC ATAGCTCTGA ATTACCGGGA CGCTGAGGGG
GATCTGGTTC GGCTGCTGTC GGATGAGGAC GTAGCGCTCA TGGTGCGGCA GGCTCGTGGC
CTCCCCTCCC AGAAGCGCCT CTTCCCCTGG AAGCTGCACA TCACGCAGAA GGACAACTAC
AGGGTCTACA ACACGATGCC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18835
Accession Version NM_013416.3
Sequence Information ORF Nucleotide Sequence (Length: 1047bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neutrophil cytosol factor 4 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC002798.1, AK290924.1 and BU076892.1. This sequence is a reference standard in the RefSeqGene project. On Dec 20, 2007 this sequence version replaced gi:47519769. Summary: The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). This isoform (2) lacks the PC (Phox and Cdc24p) motif, which may be important for the interaction with NCF2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002798.1, AK290924.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTGTGG CCCAGCAGCT GCGGGCCGAG AGTGACTTTG AACAGCTTCC GGATGATGTT 
GCCATCTCGG CCAACATTGC TGACATCGAG GAGAAGAGAG GCTTCACCAG CCACTTTGTT
TTCGTCATCG AGGTGAAGAC AAAAGGAGGA TCCAAGTACC TCATCTACCG CCGCTACCGC
CAGTTCCATG CTTTGCAGAG CAAGCTGGAG GAGCGCTTCG GGCCAGACAG CAAGAGCAGT
GCCCTGGCCT GTACCCTGCC CACACTCCCA GCCAAAGTCT ACGTGGGTGT GAAACAGGAG
ATCGCCGAGA TGCGGATACC TGCCCTCAAC GCCTACATGA AGAGCCTGCT CAGCCTGCCG
GTCTGGGTGC TGATGGATGA GGACGTCCGG ATCTTCTTTT ACCAGTCGCC CTATGACTCA
GAGCAGGTGC CCCAGGCACT CCGCCGGCTC CGCCCGCGCA CCCGGAAAGT CAAGAGCGTG
TCCCCACAGG GCAACAGCGT TGACCGCATG GCAGCTCCGA GAGCAGAGGC TCTATTTGAC
TTCACTGGAA ACAGCAAACT GGAGCTGAAT TTCAAAGCTG GAGATGTGAT CTTCCTCCTC
AGTCGGATCA ACAAAGACTG GCTGGAGGGC ACTGTCCGGG GAGCCACGGG CATCTTCCCT
CTCTCCTTCG TGAAGATCCT CAAAGACTTC CCTGAGGAGG ACGACCCCAC CAACTGGCTG
CGTTGCTACT ACTACGAAGA CACCATCAGC ACCATCAAGT CTGTGGCCTG GGAGGGAGGG
GCCTGTCCAG CCTTCCTGCC ATCCCTACGA CCACTGCCCC TCACATCACC TTCTCATGGG
TCCCTCTCCC ACTCCAAAGC CCCCAGTGGC TCCCAGATGA GCCACAATGC TGTAACAAGC
CATCAACGTC CAGGGTGGCC TGGCCAGCCT CATTCCCCTT TCCCCCACCC CACACCCCAC
TTCCAGCCTG ATGCCTCCTT ACTCCAGCCT GTCACCCCCT TAGGGACATC GCGGTGGAGG
AAGATCTCAG CAGCACTCCC CTATTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_038202.2
CDS185..1231
Misc Feature(1)122..124(+)
Misc Feature(2)239..607(+)
Misc Feature(3)281..607(+)
Misc Feature(4)356..499(+)
Misc Feature(5)356..364(+)
Misc Feature(6)458..466(+)
Misc Feature(7)644..646(+)
Misc Feature(8)704..865(+)
Misc Feature(9)719..853(+)
Misc Feature(10)782..832(+)
Misc Feature(11)896..>958(+)
Misc Feature(12)899..946(+)
Misc Feature(13)899..943(+)
Misc Feature(14)905..907(+)
Exon (1)1..216
Gene:NCF4
Gene Synonym:
Exon (2)217..301
Gene:NCF4
Gene Synonym:
Exon (3)302..455
Gene:NCF4
Gene Synonym:
Exon (4)456..526
Gene:NCF4
Gene Synonym:
Exon (5)527..654
Gene:NCF4
Gene Synonym:
Exon (6)655..712
Gene:NCF4
Gene Synonym:
Exon (7)713..811
Gene:NCF4
Gene Synonym:
Exon (8)812..1253
Gene:NCF4
Gene Synonym:
Exon (9)1254..1643
Gene:NCF4
Gene Synonym:
Translation
Position Chain Variation Link
24 24 a, g dbSNP:539979592
33 33 c, t dbSNP:565072966
38 38 c, t dbSNP:11703711
90 90 c, t dbSNP:148141762
91 91 a, g dbSNP:544072092
136 136 g, t dbSNP:772046027
147 147 c, g dbSNP:773220961
148 148 a, g dbSNP:34567417
152 152 c, t dbSNP:770775392
162 162 a, g dbSNP:776136866
177 177 c, t dbSNP:759301673
181 181 -, a dbSNP:777849995
183 183 c, t dbSNP:764810264
192 192 g, t dbSNP:752307145
207 207 a, g dbSNP:762576326
211 211 a, c, t dbSNP:372612108
212 212 a, g dbSNP:756372095
220 220 c, g dbSNP:766618219
222 222 c, t dbSNP:754276557
230 230 c, t dbSNP:755356622
234 234 c, t dbSNP:147322774
235 235 a, g dbSNP:139289225
235 235 -, g dbSNP:746841045
242 242 a, g dbSNP:575470394
247 247 c, t dbSNP:34373276
253 253 a, g dbSNP:10854695
258 258 a, g dbSNP:573525247
260 260 a, g dbSNP:541007080
262 262 c, t dbSNP:139319252
270 270 a, c, t dbSNP:13057803
271 271 c, g, t dbSNP:149998137
272 272 -, gag dbSNP:770609723
272 272 a, g dbSNP:775229266
274 274 a, g dbSNP:200822582
281 281 a, g dbSNP:768042374
282 282 g, t dbSNP:773949075
283 283 -, aggct dbSNP:776368871
283 283 a, g dbSNP:761061449
285 285 a, c, g, t dbSNP:766859628
286 286 a, c, t dbSNP:765598164
287 287 c, t dbSNP:758660640
288 288 a, c, t dbSNP:764060580
289 289 c, t dbSNP:757314998
290 290 -, ctccc dbSNP:745470237
290 290 a, c dbSNP:200818199
293 293 a, g dbSNP:199943692
307 307 c, t dbSNP:369350039
308 308 a, c, g dbSNP:371578849
311 311 a, g dbSNP:779970175
313 313 c, t dbSNP:753635134
316 316 c, g dbSNP:754759226
326 326 -, aaggaggatc dbSNP:762209908
329 329 a, g dbSNP:148715332
341 341 c, t dbSNP:747769633
346 346 a, c dbSNP:771597330
351 351 -, cccc dbSNP:144929792
353 353 c, t dbSNP:767200492
354 354 g, t dbSNP:746353194
355 355 a, c dbSNP:770239095
356 356 c, t dbSNP:143532979
357 357 a, g dbSNP:763314327
362 362 c, t dbSNP:183179978
363 363 a, g dbSNP:369847561
364 364 c, t dbSNP:762003847
372 372 a, g dbSNP:767668921
385 385 c, g dbSNP:750743658
398 398 c, t dbSNP:750577331
403 403 c, t dbSNP:373041130
404 404 a, g dbSNP:766377093
406 406 a, g, t dbSNP:377510654
409 409 a, g dbSNP:778630595
412 412 c, g, t dbSNP:188209920
413 413 a, g dbSNP:777426455
422 422 a, g dbSNP:373447489
424 424 c, t dbSNP:35431748
426 426 c, t dbSNP:780484949
437 437 a, g dbSNP:749822456
438 438 a, c dbSNP:112306225
442 442 a, g dbSNP:774613957
451 451 a, c dbSNP:748376366
454 454 a, t dbSNP:370754874
455 455 g, t dbSNP:773596460
462 462 c, t dbSNP:747292434
466 466 c, t dbSNP:771129807
467 467 a, g dbSNP:112273712
482 482 g, t dbSNP:759685640
484 484 a, g dbSNP:765046425
485 485 a, g dbSNP:775504785
487 487 c, t dbSNP:746977479
488 488 a, g dbSNP:763938344
490 490 c, t dbSNP:34072404
497 497 c, t dbSNP:756948322
498 498 a, c, g, t dbSNP:387906808
499 499 c, g dbSNP:779218017
501 501 c, t dbSNP:748676281
514 514 c, t dbSNP:373828331
515 515 a, g dbSNP:778347689
518 518 c, t dbSNP:747202725
522 522 g, t dbSNP:576610489
528 528 a, g dbSNP:567656365
529 529 c, t dbSNP:774300357
532 532 a, g dbSNP:761526575
537 537 a, g dbSNP:9610595
539 539 c, t dbSNP:377706087
542 542 c, g dbSNP:772724160
543 543 a, c, t dbSNP:760441596
544 544 a, g dbSNP:753447204
545 545 a, g, t dbSNP:759148798
547 547 c, t dbSNP:752127204
556 556 a, g, t dbSNP:368863802
568 568 c, t dbSNP:144105468
573 573 c, g dbSNP:750583738
574 574 a, g dbSNP:199642717
590 590 c, t dbSNP:780280945
591 591 c, t dbSNP:749322339
592 592 a, g dbSNP:755052012
594 594 c, t dbSNP:778761203
605 605 g, t dbSNP:748172203
613 613 a, g dbSNP:771758194
619 619 g, t dbSNP:773098220
625 625 c, g dbSNP:746619780
626 626 c, t dbSNP:770577417
627 627 a, g dbSNP:376709783
629 629 a, c dbSNP:759057034
630 630 a, g dbSNP:371098446
631 631 -, gctccgccc dbSNP:754040377
631 631 a, g, t dbSNP:764896112
635 635 c, t dbSNP:150976323
636 636 a, g dbSNP:767747416
637 637 c, t dbSNP:750746060
640 640 a, g, t dbSNP:554368196
641 641 c, t dbSNP:572795915
642 642 a, g dbSNP:35160112
643 643 c, t dbSNP:779032981
647 647 a, c dbSNP:758154907
661 661 c, t dbSNP:752773971
662 662 a, c, g dbSNP:150103256
666 666 c, t dbSNP:751507562
681 681 g, t dbSNP:757112377
682 682 c, t dbSNP:560584199
683 683 a, g dbSNP:745490664
690 690 a, g dbSNP:371308317
703 703 a, g dbSNP:779600542
710 710 a, g dbSNP:748770662
716 716 c, t dbSNP:767768687
720 720 c, t dbSNP:773281682
733 733 a, g dbSNP:760573346
737 737 a, g dbSNP:766040731
744 744 c, t dbSNP:766177098
770 770 g, t dbSNP:753529855
771 771 g, t dbSNP:754833887
774 774 a, t dbSNP:764803761
781 781 c, t dbSNP:752404521
782 782 c, t dbSNP:559758444
788 788 a, c, t dbSNP:138411102
789 789 a, c, g dbSNP:35355915
790 790 a, g dbSNP:749545374
792 792 a, t dbSNP:769074205
797 797 -, aa dbSNP:757106743
801 801 a, g dbSNP:774706034
805 805 g, t dbSNP:748453334
807 807 g, t dbSNP:772249899
808 808 a, g dbSNP:773477832
810 810 a, c dbSNP:760832946
822 822 a, g dbSNP:376569226
831 831 c, t dbSNP:146911421
832 832 a, g dbSNP:190625962
839 839 c, t dbSNP:761212596
843 843 -, ct dbSNP:780782557
843 843 c, t dbSNP:767125572
849 849 c, t dbSNP:754272966
853 853 c, t dbSNP:755594287
854 854 a, g dbSNP:200068754
859 859 c, g dbSNP:753071695
860 860 a, c dbSNP:368429862
868 868 a, c dbSNP:777800037
870 870 a, t dbSNP:747315841
872 872 g, t dbSNP:770883606
874 874 c, t dbSNP:781381492
886 886 c, t dbSNP:745954906
888 888 -, a dbSNP:745545652
889 889 c, t dbSNP:150656826
891 891 c, t dbSNP:775408197
901 901 g, t dbSNP:762676581
905 905 c, t dbSNP:768494262
906 906 a, g dbSNP:575016108
913 913 c, t dbSNP:761570088
919 919 c, g, t dbSNP:2072712
920 920 a, g dbSNP:560705177
922 922 a, g dbSNP:765774359
926 926 a, g dbSNP:753271883
927 927 c, t dbSNP:548576102
931 931 a, c dbSNP:147089818
933 933 a, g dbSNP:546475819
934 934 c, t dbSNP:757450151
936 936 c, t dbSNP:781298815
948 948 c, t dbSNP:745877776
954 954 c, g dbSNP:756196818
958 958 c, g dbSNP:377257592
963 963 a, g dbSNP:779949118
966 966 c, t dbSNP:749287293
974 974 a, g dbSNP:148129899
980 980 c, t dbSNP:141914454
981 981 c, t dbSNP:532148733
986 986 a, t dbSNP:771718040
992 992 c, t dbSNP:201021542
993 993 a, g dbSNP:150640467
996 996 c, t dbSNP:766044871
999 999 c, t dbSNP:2075939
1000 1000 a, g dbSNP:763603530
1002 1002 c, t dbSNP:764531129
1006 1006 c, t dbSNP:373460310
1020 1020 a, t dbSNP:757566507
1023 1023 c, g dbSNP:201017117
1029 1029 c, t dbSNP:750700271
1032 1032 c, t dbSNP:756178255
1040 1040 -, aa dbSNP:769387450
1043 1043 c, g dbSNP:780144703
1045 1045 a, c dbSNP:749205598
1049 1049 a, g dbSNP:754899491
1050 1050 a, g dbSNP:778749388
1052 1052 a, g dbSNP:747913896
1053 1053 a, g dbSNP:771778428
1067 1067 c, t dbSNP:772986521
1071 1071 a, g dbSNP:368807683
1078 1078 a, c dbSNP:770541268
1086 1086 a, c dbSNP:776230121
1087 1087 c, t dbSNP:200848048
1092 1092 a, g dbSNP:759148716
1098 1098 a, g dbSNP:769139490
1100 1100 c, t dbSNP:774730268
1101 1101 a, g dbSNP:775053057
1117 1117 c, t dbSNP:374914189
1119 1119 a, c dbSNP:767833960
1122 1122 c, g dbSNP:141940133
1130 1130 c, g dbSNP:750610795
1135 1135 a, c dbSNP:760939810
1139 1139 a, c, t dbSNP:766613420
1140 1140 c, t dbSNP:200504193
1152 1152 c, g dbSNP:778853862
1154 1154 a, g dbSNP:752634686
1169 1169 c, t dbSNP:758210984
1174 1174 a, t dbSNP:777751988
1178 1178 a, c dbSNP:746820593
1179 1179 a, c dbSNP:770665668
1180 1180 a, c dbSNP:369565034
1181 1181 a, c dbSNP:146292447
1182 1182 c, g, t dbSNP:202038330
1187 1187 c, g dbSNP:200347935
1192 1192 a, g dbSNP:772400898
1195 1195 a, g dbSNP:773520052
1196 1196 c, t dbSNP:761060692
1197 1197 a, g dbSNP:766525712
1200 1200 a, g dbSNP:754075839
1209 1209 g, t dbSNP:759649020
1225 1225 c, t dbSNP:374646787
1227 1227 a, g dbSNP:752651381
1228 1228 c, t dbSNP:139432640
1230 1230 c, g dbSNP:777882570
1238 1238 g, t dbSNP:751332212
1242 1242 a, g dbSNP:144124883
1245 1245 a, g dbSNP:780975917
1254 1254 g, t dbSNP:145005349
1255 1255 c, t dbSNP:200598824
1256 1256 a, g dbSNP:769839943
1268 1268 g, t dbSNP:775794915
1272 1272 a, g dbSNP:763111423
1274 1274 a, g dbSNP:764332022
1276 1276 a, g dbSNP:774395484
1278 1278 a, t dbSNP:761887926
1291 1291 c, g, t dbSNP:148661727
1292 1292 a, g dbSNP:750147229
1294 1294 a, g dbSNP:755943893
1296 1296 c, t dbSNP:765893996
1297 1297 a, g dbSNP:753588526
1301 1301 a, t dbSNP:754480497
1303 1303 a, g dbSNP:778435771
1306 1306 a, g dbSNP:752040969
1308 1308 a, c, t dbSNP:201317613
1309 1309 c, g dbSNP:781723897
1315 1315 c, g dbSNP:199500127
1316 1316 a, g, t dbSNP:554387451
1326 1326 a, g dbSNP:11552115
1335 1335 c, t dbSNP:567611718
1336 1336 a, g, t dbSNP:201552121
1337 1337 g, t dbSNP:762012398
1340 1340 a, c, t dbSNP:5995361
1341 1341 a, g dbSNP:200914046
1342 1342 c, t dbSNP:766267228
1351 1351 c, t dbSNP:546311292
1352 1352 a, g dbSNP:141160114
1358 1358 c, g dbSNP:764893385
1359 1359 c, t dbSNP:752165248
1360 1360 a, c, t dbSNP:757937260
1361 1361 a, g dbSNP:751005224
1363 1363 g, t dbSNP:199618052
1369 1369 a, c dbSNP:199686562
1370 1370 c, t dbSNP:200966942
1381 1381 c, t dbSNP:780513211
1382 1382 a, g, t dbSNP:371008750
1386 1386 c, t dbSNP:779285588
1389 1389 c, t dbSNP:1858
1395 1395 a, g dbSNP:772255576
1407 1407 a, c dbSNP:370312224
1409 1409 c, t dbSNP:367876433
1410 1410 a, g dbSNP:376148807
1436 1436 a, g dbSNP:776362467
1437 1437 c, t dbSNP:759359854
1439 1439 c, t dbSNP:147550660
1440 1440 a, g dbSNP:28669668
1442 1442 g, t dbSNP:762365393
1443 1443 -, gcc dbSNP:775117377
1455 1455 c, t dbSNP:763700539
1456 1456 a, g dbSNP:750868959
1460 1460 -, c dbSNP:36023462
1462 1462 c, t dbSNP:756592059
1466 1466 c, g dbSNP:767020866
1468 1468 a, g dbSNP:754185606
1472 1472 c, t dbSNP:755408986
1479 1479 a, t dbSNP:557549740
1497 1497 a, g dbSNP:748451564
1512 1512 c, t dbSNP:781057169
1527 1527 c, g dbSNP:576231383
1529 1529 -, g dbSNP:34155264
1545 1545 c, g dbSNP:182197208
1550 1550 c, t dbSNP:541831946
1554 1554 c, t dbSNP:748052138
1571 1571 c, t dbSNP:186232557
1572 1572 a, g dbSNP:562129519
1582 1582 a, t dbSNP:561904370
1621 1621 a, c dbSNP:529161346
1631 1631 a, t dbSNP:540990854

Target ORF information:

RefSeq Version NM_013416
Organism Homo sapiens (human)
Definition Homo sapiens neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_013416

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTGTGG CCCAGCAGCT GCGGGCCGAG AGTGACTTTG AACAGCTTCC GGATGATGTT 
GCCATCTCGG CCAACATTGC TGACATCGAG GAGAAGAGAG GCTTCACCAG CCACTTTGTT
TTCGTCATCG AGGTGAAGAC AAAAGGAGGA TCCAAGTACC TCATCTACCG CCGCTACCGC
CAGTTCCATG CTTTGCAGAG CAAGCTGGAG GAGCGCTTCG GGCCAGACAG CAAGAGCAGT
GCCCTGGCCT GTACCCTGCC CACACTCCCA GCCAAAGTCT ACGTGGGTGT GAAACAGGAG
ATCGCCGAGA TGCGGATACC TGCCCTCAAC GCCTACATGA AGAGCCTGCT CAGCCTGCCG
GTCTGGGTGC TGATGGATGA GGACGTCCGG ATCTTCTTTT ACCAGTCGCC CTATGACTCA
GAGCAGGTGC CCCAGGCACT CCGCCGGCTC CGCCCGCGCA CCCGGAAAGT CAAGAGCGTG
TCCCCACAGG GCAACAGCGT TGACCGCATG GCAGCTCCGA GAGCAGAGGC TCTATTTGAC
TTCACTGGAA ACAGCAAACT GGAGCTGAAT TTCAAAGCTG GAGATGTGAT CTTCCTCCTC
AGTCGGATCA ACAAAGACTG GCTGGAGGGC ACTGTCCGGG GAGCCACGGG CATCTTCCCT
CTCTCCTTCG TGAAGATCCT CAAAGACTTC CCTGAGGAGG ACGACCCCAC CAACTGGCTG
CGTTGCTACT ACTACGAAGA CACCATCAGC ACCATCAAGT CTGTGGCCTG GGAGGGAGGG
GCCTGTCCAG CCTTCCTGCC ATCCCTACGA CCACTGCCCC TCACATCACC TTCTCATGGG
TCCCTCTCCC ACTCCAAAGC CCCCAGTGGC TCCCAGATGA GCCACAATGC TGTAACAAGC
CATCAACGTC CAGGGTGGCC TGGCCAGCCT CATTCCCCTT TCCCCCACCC CACACCCCAC
TTCCAGCCTG ATGCCTCCTT ACTCCAGCCT GTCACCCCCT TAGGGACATC GCGGTGGAGG
AAGATCTCAG CAGCACTCCC CTATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16465
Accession Version NM_000631.4
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neutrophil cytosol factor 4 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC002798.1, BX355636.2 and BU076892.1. On Dec 20, 2007 this sequence version replaced gi:47519797. Summary: The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) lacks an internal coding segment compared to variant 2, which leads to a translation frameshift. The resulting isoform (1) has a distinct and shorter C-terminus, as compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK223324.1, CR456528.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGTGG CCCAGCAGCT GCGGGCCGAG AGTGACTTTG AACAGCTTCC GGATGATGTT 
GCCATCTCGG CCAACATTGC TGACATCGAG GAGAAGAGAG GCTTCACCAG CCACTTTGTT
TTCGTCATCG AGGTGAAGAC AAAAGGAGGA TCCAAGTACC TCATCTACCG CCGCTACCGC
CAGTTCCATG CTTTGCAGAG CAAGCTGGAG GAGCGCTTCG GGCCAGACAG CAAGAGCAGT
GCCCTGGCCT GTACCCTGCC CACACTCCCA GCCAAAGTCT ACGTGGGTGT GAAACAGGAG
ATCGCCGAGA TGCGGATACC TGCCCTCAAC GCCTACATGA AGAGCCTGCT CAGCCTGCCG
GTCTGGGTGC TGATGGATGA GGACGTCCGG ATCTTCTTTT ACCAGTCGCC CTATGACTCA
GAGCAGGTGC CCCAGGCACT CCGCCGGCTC CGCCCGCGCA CCCGGAAAGT CAAGAGCGTG
TCCCCACAGG GCAACAGCGT TGACCGCATG GCAGCTCCGA GAGCAGAGGC TCTATTTGAC
TTCACTGGAA ACAGCAAACT GGAGCTGAAT TTCAAAGCTG GAGATGTGAT CTTCCTCCTC
AGTCGGATCA ACAAAGACTG GCTGGAGGGC ACTGTCCGGG GAGCCACGGG CATCTTCCCT
CTCTCCTTCG TGAAGATCCT CAAAGACTTC CCTGAGGAGG ACGACCCCAC CAACTGGCTG
CGTTGCTACT ACTACGAAGA CACCATCAGC ACCATCAAGG ACATCGCGGT GGAGGAAGAT
CTCAGCAGCA CTCCCCTATT GAAAGACCTG CTGGAGCTCA CAAGGCGGGA GTTCCAGAGA
GAGGACATAG CTCTGAATTA CCGGGACGCT GAGGGGGATC TGGTTCGGCT GCTGTCGGAT
GAGGACGTAG CGCTCATGGT GCGGCAGGCT CGTGGCCTCC CCTCCCAGAA GCGCCTCTTC
CCCTGGAAGC TGCACATCAC GCAGAAGGAC AACTACAGGG TCTACAACAC GATGCCATGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000622.2
CDS185..1204
Misc Feature(1)122..124(+)
Misc Feature(2)239..607(+)
Misc Feature(3)281..607(+)
Misc Feature(4)356..499(+)
Misc Feature(5)356..364(+)
Misc Feature(6)458..466(+)
Misc Feature(7)644..646(+)
Misc Feature(8)644..646(+)
Misc Feature(9)704..865(+)
Misc Feature(10)719..853(+)
Misc Feature(11)782..832(+)
Misc Feature(12)896..1171(+)
Misc Feature(13)953..1150(+)
Misc Feature(14)1049..1090(+)
Misc Feature(15)1127..1129(+)
Exon (1)1..216
Gene:NCF4
Gene Synonym:
Exon (2)217..301
Gene:NCF4
Gene Synonym:
Exon (3)302..455
Gene:NCF4
Gene Synonym:
Exon (4)456..526
Gene:NCF4
Gene Synonym:
Exon (5)527..654
Gene:NCF4
Gene Synonym:
Exon (6)655..712
Gene:NCF4
Gene Synonym:
Exon (7)713..811
Gene:NCF4
Gene Synonym:
Exon (8)812..942
Gene:NCF4
Gene Synonym:
Exon (9)943..1008
Gene:NCF4
Gene Synonym:
Exon (10)1009..1398
Gene:NCF4
Gene Synonym:
Translation
Position Chain Variation Link
24 24 a, g dbSNP:539979592
33 33 c, t dbSNP:565072966
38 38 c, t dbSNP:11703711
90 90 c, t dbSNP:148141762
91 91 a, g dbSNP:544072092
136 136 g, t dbSNP:772046027
147 147 c, g dbSNP:773220961
148 148 a, g dbSNP:34567417
152 152 c, t dbSNP:770775392
162 162 a, g dbSNP:776136866
177 177 c, t dbSNP:759301673
181 181 -, a dbSNP:777849995
183 183 c, t dbSNP:764810264
192 192 g, t dbSNP:752307145
207 207 a, g dbSNP:762576326
211 211 a, c, t dbSNP:372612108
212 212 a, g dbSNP:756372095
220 220 c, g dbSNP:766618219
222 222 c, t dbSNP:754276557
230 230 c, t dbSNP:755356622
234 234 c, t dbSNP:147322774
235 235 a, g dbSNP:139289225
235 235 -, g dbSNP:746841045
242 242 a, g dbSNP:575470394
247 247 c, t dbSNP:34373276
253 253 a, g dbSNP:10854695
258 258 a, g dbSNP:573525247
260 260 a, g dbSNP:541007080
262 262 c, t dbSNP:139319252
270 270 a, c, t dbSNP:13057803
271 271 c, g, t dbSNP:149998137
272 272 -, gag dbSNP:770609723
272 272 a, g dbSNP:775229266
274 274 a, g dbSNP:200822582
281 281 a, g dbSNP:768042374
282 282 g, t dbSNP:773949075
283 283 -, aggct dbSNP:776368871
283 283 a, g dbSNP:761061449
285 285 a, c, g, t dbSNP:766859628
286 286 a, c, t dbSNP:765598164
287 287 c, t dbSNP:758660640
288 288 a, c, t dbSNP:764060580
289 289 c, t dbSNP:757314998
290 290 -, ctccc dbSNP:745470237
290 290 a, c dbSNP:200818199
293 293 a, g dbSNP:199943692
307 307 c, t dbSNP:369350039
308 308 a, c, g dbSNP:371578849
311 311 a, g dbSNP:779970175
313 313 c, t dbSNP:753635134
316 316 c, g dbSNP:754759226
326 326 -, aaggaggatc dbSNP:762209908
329 329 a, g dbSNP:148715332
341 341 c, t dbSNP:747769633
346 346 a, c dbSNP:771597330
351 351 -, cccc dbSNP:144929792
353 353 c, t dbSNP:767200492
354 354 g, t dbSNP:746353194
355 355 a, c dbSNP:770239095
356 356 c, t dbSNP:143532979
357 357 a, g dbSNP:763314327
362 362 c, t dbSNP:183179978
363 363 a, g dbSNP:369847561
364 364 c, t dbSNP:762003847
372 372 a, g dbSNP:767668921
385 385 c, g dbSNP:750743658
398 398 c, t dbSNP:750577331
403 403 c, t dbSNP:373041130
404 404 a, g dbSNP:766377093
406 406 a, g, t dbSNP:377510654
409 409 a, g dbSNP:778630595
412 412 c, g, t dbSNP:188209920
413 413 a, g dbSNP:777426455
422 422 a, g dbSNP:373447489
424 424 c, t dbSNP:35431748
426 426 c, t dbSNP:780484949
437 437 a, g dbSNP:749822456
438 438 a, c dbSNP:112306225
442 442 a, g dbSNP:774613957
451 451 a, c dbSNP:748376366
454 454 a, t dbSNP:370754874
455 455 g, t dbSNP:773596460
462 462 c, t dbSNP:747292434
466 466 c, t dbSNP:771129807
467 467 a, g dbSNP:112273712
482 482 g, t dbSNP:759685640
484 484 a, g dbSNP:765046425
485 485 a, g dbSNP:775504785
487 487 c, t dbSNP:746977479
488 488 a, g dbSNP:763938344
490 490 c, t dbSNP:34072404
497 497 c, t dbSNP:756948322
498 498 a, c, g, t dbSNP:387906808
499 499 c, g dbSNP:779218017
501 501 c, t dbSNP:748676281
514 514 c, t dbSNP:373828331
515 515 a, g dbSNP:778347689
518 518 c, t dbSNP:747202725
522 522 g, t dbSNP:576610489
528 528 a, g dbSNP:567656365
529 529 c, t dbSNP:774300357
532 532 a, g dbSNP:761526575
537 537 a, g dbSNP:9610595
539 539 c, t dbSNP:377706087
542 542 c, g dbSNP:772724160
543 543 a, c, t dbSNP:760441596
544 544 a, g dbSNP:753447204
545 545 a, g, t dbSNP:759148798
547 547 c, t dbSNP:752127204
556 556 a, g, t dbSNP:368863802
568 568 c, t dbSNP:144105468
573 573 c, g dbSNP:750583738
574 574 a, g dbSNP:199642717
590 590 c, t dbSNP:780280945
591 591 c, t dbSNP:749322339
592 592 a, g dbSNP:755052012
594 594 c, t dbSNP:778761203
605 605 g, t dbSNP:748172203
613 613 a, g dbSNP:771758194
619 619 g, t dbSNP:773098220
625 625 c, g dbSNP:746619780
626 626 c, t dbSNP:770577417
627 627 a, g dbSNP:376709783
629 629 a, c dbSNP:759057034
630 630 a, g dbSNP:371098446
631 631 -, gctccgccc dbSNP:754040377
631 631 a, g, t dbSNP:764896112
635 635 c, t dbSNP:150976323
636 636 a, g dbSNP:767747416
637 637 c, t dbSNP:750746060
640 640 a, g, t dbSNP:554368196
641 641 c, t dbSNP:572795915
642 642 a, g dbSNP:35160112
643 643 c, t dbSNP:779032981
647 647 a, c dbSNP:758154907
661 661 c, t dbSNP:752773971
662 662 a, c, g dbSNP:150103256
666 666 c, t dbSNP:751507562
681 681 g, t dbSNP:757112377
682 682 c, t dbSNP:560584199
683 683 a, g dbSNP:745490664
690 690 a, g dbSNP:371308317
703 703 a, g dbSNP:779600542
710 710 a, g dbSNP:748770662
716 716 c, t dbSNP:767768687
720 720 c, t dbSNP:773281682
733 733 a, g dbSNP:760573346
737 737 a, g dbSNP:766040731
744 744 c, t dbSNP:766177098
770 770 g, t dbSNP:753529855
771 771 g, t dbSNP:754833887
774 774 a, t dbSNP:764803761
781 781 c, t dbSNP:752404521
782 782 c, t dbSNP:559758444
788 788 a, c, t dbSNP:138411102
789 789 a, c, g dbSNP:35355915
790 790 a, g dbSNP:749545374
792 792 a, t dbSNP:769074205
797 797 -, aa dbSNP:757106743
801 801 a, g dbSNP:774706034
805 805 g, t dbSNP:748453334
807 807 g, t dbSNP:772249899
808 808 a, g dbSNP:773477832
810 810 a, c dbSNP:760832946
822 822 a, g dbSNP:376569226
831 831 c, t dbSNP:146911421
832 832 a, g dbSNP:190625962
839 839 c, t dbSNP:761212596
843 843 -, ct dbSNP:780782557
843 843 c, t dbSNP:767125572
849 849 c, t dbSNP:754272966
853 853 c, t dbSNP:755594287
854 854 a, g dbSNP:200068754
859 859 c, g dbSNP:753071695
860 860 a, c dbSNP:368429862
868 868 a, c dbSNP:777800037
870 870 a, t dbSNP:747315841
872 872 g, t dbSNP:770883606
874 874 c, t dbSNP:781381492
886 886 c, t dbSNP:745954906
888 888 -, a dbSNP:745545652
889 889 c, t dbSNP:150656826
891 891 c, t dbSNP:775408197
901 901 g, t dbSNP:762676581
905 905 c, t dbSNP:768494262
906 906 a, g dbSNP:575016108
913 913 c, t dbSNP:761570088
919 919 c, g, t dbSNP:2072712
920 920 a, g dbSNP:560705177
922 922 a, g dbSNP:765774359
926 926 a, g dbSNP:753271883
927 927 c, t dbSNP:548576102
931 931 a, c dbSNP:147089818
933 933 a, g dbSNP:546475819
934 934 c, t dbSNP:757450151
936 936 c, t dbSNP:781298815
947 947 a, g dbSNP:772400898
950 950 a, g dbSNP:773520052
951 951 c, t dbSNP:761060692
952 952 a, g dbSNP:766525712
955 955 a, g dbSNP:754075839
964 964 g, t dbSNP:759649020
980 980 c, t dbSNP:374646787
982 982 a, g dbSNP:752651381
983 983 c, t dbSNP:139432640
985 985 c, g dbSNP:777882570
993 993 g, t dbSNP:751332212
997 997 a, g dbSNP:144124883
1000 1000 a, g dbSNP:780975917
1009 1009 g, t dbSNP:145005349
1010 1010 c, t dbSNP:200598824
1011 1011 a, g dbSNP:769839943
1023 1023 g, t dbSNP:775794915
1027 1027 a, g dbSNP:763111423
1029 1029 a, g dbSNP:764332022
1031 1031 a, g dbSNP:774395484
1033 1033 a, t dbSNP:761887926
1046 1046 c, g, t dbSNP:148661727
1047 1047 a, g dbSNP:750147229
1049 1049 a, g dbSNP:755943893
1051 1051 c, t dbSNP:765893996
1052 1052 a, g dbSNP:753588526
1056 1056 a, t dbSNP:754480497
1058 1058 a, g dbSNP:778435771
1061 1061 a, g dbSNP:752040969
1063 1063 a, c, t dbSNP:201317613
1064 1064 c, g dbSNP:781723897
1070 1070 c, g dbSNP:199500127
1071 1071 a, g, t dbSNP:554387451
1081 1081 a, g dbSNP:11552115
1090 1090 c, t dbSNP:567611718
1091 1091 a, g, t dbSNP:201552121
1092 1092 g, t dbSNP:762012398
1095 1095 a, c, t dbSNP:5995361
1096 1096 a, g dbSNP:200914046
1097 1097 c, t dbSNP:766267228
1106 1106 c, t dbSNP:546311292
1107 1107 a, g dbSNP:141160114
1113 1113 c, g dbSNP:764893385
1114 1114 c, t dbSNP:752165248
1115 1115 a, c, t dbSNP:757937260
1116 1116 a, g dbSNP:751005224
1118 1118 g, t dbSNP:199618052
1124 1124 a, c dbSNP:199686562
1125 1125 c, t dbSNP:200966942
1136 1136 c, t dbSNP:780513211
1137 1137 a, g, t dbSNP:371008750
1141 1141 c, t dbSNP:779285588
1144 1144 c, t dbSNP:1858
1150 1150 a, g dbSNP:772255576
1162 1162 a, c dbSNP:370312224
1164 1164 c, t dbSNP:367876433
1165 1165 a, g dbSNP:376148807
1191 1191 a, g dbSNP:776362467
1192 1192 c, t dbSNP:759359854
1194 1194 c, t dbSNP:147550660
1195 1195 a, g dbSNP:28669668
1197 1197 g, t dbSNP:762365393
1198 1198 -, gcc dbSNP:775117377
1210 1210 c, t dbSNP:763700539
1211 1211 a, g dbSNP:750868959
1215 1215 -, c dbSNP:36023462
1217 1217 c, t dbSNP:756592059
1221 1221 c, g dbSNP:767020866
1223 1223 a, g dbSNP:754185606
1227 1227 c, t dbSNP:755408986
1234 1234 a, t dbSNP:557549740
1252 1252 a, g dbSNP:748451564
1267 1267 c, t dbSNP:781057169
1282 1282 c, g dbSNP:576231383
1284 1284 -, g dbSNP:34155264
1300 1300 c, g dbSNP:182197208
1305 1305 c, t dbSNP:541831946
1309 1309 c, t dbSNP:748052138
1326 1326 c, t dbSNP:186232557
1327 1327 a, g dbSNP:562129519
1337 1337 a, t dbSNP:561904370
1376 1376 a, c dbSNP:529161346
1386 1386 a, t dbSNP:540990854

Target ORF information:

RefSeq Version NM_000631
Organism Homo sapiens (human)
Definition Homo sapiens neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000631

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGTGG CCCAGCAGCT GCGGGCCGAG AGTGACTTTG AACAGCTTCC GGATGATGTT 
GCCATCTCGG CCAACATTGC TGACATCGAG GAGAAGAGAG GCTTCACCAG CCACTTTGTT
TTCGTCATCG AGGTGAAGAC AAAAGGAGGA TCCAAGTACC TCATCTACCG CCGCTACCGC
CAGTTCCATG CTTTGCAGAG CAAGCTGGAG GAGCGCTTCG GGCCAGACAG CAAGAGCAGT
GCCCTGGCCT GTACCCTGCC CACACTCCCA GCCAAAGTCT ACGTGGGTGT GAAACAGGAG
ATCGCCGAGA TGCGGATACC TGCCCTCAAC GCCTACATGA AGAGCCTGCT CAGCCTGCCG
GTCTGGGTGC TGATGGATGA GGACGTCCGG ATCTTCTTTT ACCAGTCGCC CTATGACTCA
GAGCAGGTGC CCCAGGCACT CCGCCGGCTC CGCCCGCGCA CCCGGAAAGT CAAGAGCGTG
TCCCCACAGG GCAACAGCGT TGACCGCATG GCAGCTCCGA GAGCAGAGGC TCTATTTGAC
TTCACTGGAA ACAGCAAACT GGAGCTGAAT TTCAAAGCTG GAGATGTGAT CTTCCTCCTC
AGTCGGATCA ACAAAGACTG GCTGGAGGGC ACTGTCCGGG GAGCCACGGG CATCTTCCCT
CTCTCCTTCG TGAAGATCCT CAAAGACTTC CCTGAGGAGG ACGACCCCAC CAACTGGCTG
CGTTGCTACT ACTACGAAGA CACCATCAGC ACCATCAAGG ACATCGCGGT GGAGGAAGAT
CTCAGCAGCA CTCCCCTATT GAAAGACCTG CTGGAGCTCA CAAGGCGGGA GTTCCAGAGA
GAGGACATAG CTCTGAATTA CCGGGACGCT GAGGGGGATC TGGTTCGGCT GCTGTCGGAT
GAGGACGTAG CGCTCATGGT GCGGCAGGCT CGTGGCCTCC CCTCCCAGAA GCGCCTCTTC
CCCTGGAAGC TGCACATCAC GCAGAAGGAC AACTACAGGG TCTACAACAC GATGCCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.