×

NF1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol NF1
Entrez Gene ID 4763
Full Name neurofibromin 1
Synonyms NFNS, VRNF, WSS
General protein information
Preferred Names
neurofibromin
Names
neurofibromin
neurofibromatosis-related protein NF-1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q11.2

Summary This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

613113

Disorder Html: Neurofibromatosis, type 1, 162200 (3); Leukemia, juvenile

mRNA and Protein(s)

mRNA Protein Name
XM_006721922 XP_006721985 neurofibromin isoform X1
XM_011524852 XP_011523154 neurofibromin isoform X2
XM_005257983 XP_005258040 neurofibromin isoform X3
XM_011524853 XP_011523155 neurofibromin isoform X4
XM_011524854 XP_011523156 neurofibromin isoform X4
XM_011524855 XP_011523157 neurofibromin isoform X4
XM_011524856 XP_011523158 neurofibromin isoform X4
XM_006721923 XP_006721986 neurofibromin isoform X4
XM_006721924 XP_006721987 neurofibromin isoform X5
XM_006721925 XP_006721988 neurofibromin isoform X6
XM_005257984 XP_005258041 neurofibromin isoform X7
XM_011524857 XP_011523159 neurofibromin isoform X8
XM_006721926 XP_006721989 neurofibromin isoform X9
XM_006721927 XP_006721990 neurofibromin isoform X9
XM_006721928 XP_006721991 neurofibromin isoform X10
NM_000267 NP_000258 neurofibromin isoform 2
NM_001042492 NP_001035957 neurofibromin isoform 1
NM_001128147 NP_001121619 neurofibromin isoform 3



Homo sapiens (human) NF1 NP_001035957.1
Pan troglodytes (chimpanzee) NF1 XP_511395.2
Macaca mulatta (Rhesus monkey) NF1 XP_002800429.1
Canis lupus familiaris (dog) NF1 XP_537738.3
Mus musculus (house mouse) Nf1 NP_035027.1
Rattus norvegicus (Norway rat) Nf1 NP_036741.1
Gallus gallus (chicken) NF1 XP_004946843.1
Danio rerio (zebrafish) nf1b XP_692937.4
Drosophila melanogaster (fruit fly) Nf1 NP_733132.2
Xenopus (Silurana) tropicalis (western clawed frog) nf1 XP_002936865.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu50427 XM_006721922 PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu59245 XM_011524852 PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu50428 XM_005257983 PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu50429 XM_011524853 PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu50429 XM_011524854 PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu50429 XM_011524855 PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu50429 XM_011524856 PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu50429 XM_006721923 PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu50430 XM_006721924 PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu50431 XM_006721925 PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu50432 XM_005257984 PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu59246 XM_011524857 PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu50433 XM_006721926 PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu50433 XM_006721927 PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu50434 XM_006721928 PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X15, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $811.30
$1159.00
OHu24060 NM_000267 Homo sapiens neurofibromin 1 (NF1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu23641 NM_001042492 Homo sapiens neurofibromin 1 (NF1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu26980 NM_001128147 Homo sapiens neurofibromin 1 (NF1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu50427
Clone ID Related Accession (Same CDS sequence) XM_006721922
Accession Version XM_006721922.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8604bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neurofibromin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
8581
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
CGGAGGTTCA AAACTGGTCA AATCAATGGT GATTTGCTGA TATACCATGT CTTACTGACT
TTAAAGCCAT ATTATGCAAA GCCATATGAA ATTGTAGTGG ACCTTACCCA TACCGGGCCT
AGCAATCGCT TTAAAACAGA CTTTCTCTCT AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT
TACGACAACG TCTCCGCAGT CTATATCTAT AACTGTAACT CCTGGGTCAG GGAGTACACC
AAGTATCATG AGCGGCTGCT GACTGGCCTC AAAGGTAGCA AAAGGCTTGT TTTCATAGAC
TGTCCTGGGA AACTGGCTGA GCACATAGAG CATGAACAAC AGAAACTACC TGCTGCCACC
TTGGCTTTAG AAGAGGACCT GAAGGTATTC CACAATGCTC TCAAGCTAGC TCACAAAGAC
ACCAAAGTTT CTATTAAAGT TGGTTCTACT GCTGTCCAAG TAACTTCAGC AGAGCGAACA
AAAGTCCTAG GGCAATCAGT CTTTCTAAAT GACATTTATT ATGCTTCGGA AATTGAAGAA
ATCTGCCTAG TAGATGAGAA CCAGTTCACC TTAACCATTG CAAACCAGGG CACGCCGCTC
ACCTTCATGC ACCAGGAGTG TGAAGCCATT GTCCAGTCTA TCATTCATAT CCGGACCCGC
TGGGAACTGT CACAGCCCGA CTCTATCCCC CAACACACCA AGATTCGGCC AAAAGATGTC
CCTGGGACAC TGCTCAATAT CGCATTACTT AATTTAGGCA GTTCTGACCC GAGTTTACGG
TCAGCTGCCT ATAATCTTCT GTGTGCCTTA ACTTGTACCT TTAATTTAAA AATCGAGGGC
CAGTTACTAG AGACATCAGG TTTATGTATC CCTGCCAACA ACACCCTCTT TATTGTCTCT
ATTAGTAAGA CACTGGCAGC CAATGAGCCA CACCTCACGT TAGAATTTTT GGAAGAGTGT
ATTTCTGGAT TTAGCAAATC TAGTATTGAA TTGAAACACC TTTGTTTGGA ATACATGACT
CCATGGCTGT CAAATCTAGT TCGTTTTTGC AAGCATAATG ATGATGCCAA ACGACAAAGA
GTTACTGCTA TTCTTGACAA GCTGATAACA ATGACCATCA ATGAAAAACA GATGTACCCA
TCTATTCAAG CAAAAATATG GGGAAGCCTT GGGCAGATTA CAGATCTGCT TGATGTTGTA
CTAGACAGTT TCATCAAAAC CAGTGCAACA GGTGGCTTGG GATCAATAAA AGCTGAGGTG
ATGGCAGATA CTGCTGTAGC TTTGGCTTCT GGAAATGTGA AATTGGTTTC AAGCAAGGTT
ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACATGCTTAT CTCCAACTCC TACTTTAGAA
CAACATCTTA TGTGGGATGA TATTGCTATT TTAGCACGCT ACATGCTGAT GCTGTCCTTC
AACAATTCCC TTGATGTGGC AGCTCATCTT CCCTACCTCT TCCACGTTGT TACTTTCTTA
GTAGCCACAG GTCCGCTCTC CCTTAGAGCT TCCACACATG GACTGGTCAT TAATATCATT
CACTCTCTGT GTACTTGTTC ACAGCTTCAT TTTAGTGAAG AGACCAAGCA AGTTTTGAGA
CTCAGTCTGA CAGAGTTCTC ATTACCCAAA TTTTACTTGC TGTTTGGCAT TAGCAAAGTC
AAGTCAGCTG CTGTCATTGC CTTCCGTTCC AGTTACCGGG ACAGGTCATT CTCTCCTGGC
TCCTATGAGA GAGAGACTTT TGCTTTGACA TCCTTGGAAA CAGTCACAGA AGCTTTGTTG
GAGATCATGG AGGCATGCAT GAGAGATATT CCAACGTGCA AGTGGCTGGA CCAGTGGACA
GAACTAGCTC AAAGATTTGC ATTCCAATAT AATCCATCCC TGCAACCAAG AGCTCTTGTT
GTCTTTGGGT GTATTAGCAA ACGAGTGTCT CATGGGCAGA TAAAGCAGAT AATCCGTATT
CTTAGCAAGG CACTTGAGAG TTGCTTAAAA GGACCTGACA CTTACAACAG TCAAGTTCTG
ATAGAAGCTA CAGTAATAGC ACTAACCAAA TTACAGCCAC TTCTTAATAA GGACTCGCCT
CTGCACAAAG CCCTCTTTTG GGTAGCTGTG GCTGTGCTGC AGCTTGATGA GGTCAACTTG
TATTCAGCAG GTACCGCACT TCTTGAACAA AACCTGCATA CTTTAGATAG TCTCCGTATA
TTCAATGACA AGAGTCCAGA GGAAGTATTT ATGGCAATCC GGAATCCTCT GGAGTGGCAC
TGCAAGCAAA TGGATCATTT TGTTGGACTC AATTTCAACT CTAACTTTAA CTTTGCATTG
GTTGGACACC TTTTAAAAGG GTACAGGCAT CCTTCACCTG CTATTGTTGC AAGAACAGTC
AGAATTTTAC ATACACTACT AACTCTGGTT AACAAACACA GAAATTGTGA CAAATTTGAA
GTGAATACAC AGAGCGTGGC CTACTTAGCA GCTTTACTTA CAGTGTCTGA AGAAGTTCGA
AGTCGCTGCA GCCTAAAACA TAGAAAGTCA CTTCTTCTTA CTGATATTTC AATGGAAAAT
GTTCCTATGG ATACATATCC CATTCATCAT GGTGACCCTT CCTATAGGAC ACTAAAGGAG
ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT GAAGGATACC TTGCAGCCAC CTATCCAACT
GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA TCCATGAGCC TGGACATGGG GCAACCTTCT
CAGGCCAACA CTAAGAAGTT GCTTGGAACA AGGAAAAGTT TTGATCACTT GATATCAGAC
ACAAAGGCTC CTAAAAGGCA AGAAATGGAA TCAGGGATCA CAACACCCCC CAAAATGAGG
AGAGTAGCAG AAACTGATTA TGAAATGGAA ACTCAGAGGA TTTCCTCATC ACAACAGCAC
CCACATTTAC GTAAAGTTTC AGTGTCTGAA TCAAATGTTC TCTTGGATGA AGAAGTACTT
ACTGATCCGA AGATCCAGGC GCTGCTTCTT ACTGTTCTAG CTACACTGGT AAAATATACC
ACAGATGAGT TTGATCAACG AATTCTTTAT GAATACTTAG CAGAGGCCAG TGTTGTGTTT
CCCAAAGTCT TTCCTGTTGT GCATAATTTG TTGGACTCTA AGATCAACAC CCTGTTATCA
TTGTGCCAAG ATCCAAATTT GTTAAATCCA ATCCATGGAA TTGTGCAGAG TGTGGTGTAC
CATGAAGAAT CCCCACCACA ATACCAAACA TCTTACCTGC AAAGTTTTGG TTTTAATGGC
TTGTGGCGGT TTGCAGGACC GTTTTCAAAG CAAACACAAA TTCCAGACTA TGCTGAGCTT
ATTGTTAAGT TTCTTGATGC CTTGATTGAC ACGTACCTGC CTGGAATTGA TGAAGAAACC
AGTGAAGAAT CCCTCCTGAC TCCCACATCT CCTTACCCTC CTGCACTGCA GAGCCAGCTT
AGTATCACTG CCAACCTTAA CCTTTCTAAT TCCATGACCT CACTTGCAAC TTCCCAGCAT
TCCCCAGCTT CTCTGCCTTG CTCTAAATCA GCAGTTTTCA TGCAGCTGTT CCCTCATCAA
GGAATCGACA AGGAGAACGT TGAACTCTCC CCTACCACTG GCCACTGTAA CAGTGGACGA
ACTCGCCACG GATCCGCAAG CCAAGTGCAG AAGCAAAGAA GCGCTGGCAG TTTCAAACGT
AATAGCATTA AGAAGATCGT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006721985.1
CDS334..8937
Misc Feature(1)3922..5034(+)
Misc Feature(2)3955..5016(+)
Misc Feature(3)4069..4737(+)
Misc Feature(4)5104..5547(+)
Misc Feature(5)5173..5478(+)
Misc Feature(6)5377..5475(+)
Misc Feature(7)5545..5874(+)
Misc Feature(8)5545..5874(+)
Translation

Target ORF information:

RefSeq Version XM_006721922
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006721922

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
8581
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
CGGAGGTTCA AAACTGGTCA AATCAATGGT GATTTGCTGA TATACCATGT CTTACTGACT
TTAAAGCCAT ATTATGCAAA GCCATATGAA ATTGTAGTGG ACCTTACCCA TACCGGGCCT
AGCAATCGCT TTAAAACAGA CTTTCTCTCT AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT
TACGACAACG TCTCCGCAGT CTATATCTAT AACTGTAACT CCTGGGTCAG GGAGTACACC
AAGTATCATG AGCGGCTGCT GACTGGCCTC AAAGGTAGCA AAAGGCTTGT TTTCATAGAC
TGTCCTGGGA AACTGGCTGA GCACATAGAG CATGAACAAC AGAAACTACC TGCTGCCACC
TTGGCTTTAG AAGAGGACCT GAAGGTATTC CACAATGCTC TCAAGCTAGC TCACAAAGAC
ACCAAAGTTT CTATTAAAGT TGGTTCTACT GCTGTCCAAG TAACTTCAGC AGAGCGAACA
AAAGTCCTAG GGCAATCAGT CTTTCTAAAT GACATTTATT ATGCTTCGGA AATTGAAGAA
ATCTGCCTAG TAGATGAGAA CCAGTTCACC TTAACCATTG CAAACCAGGG CACGCCGCTC
ACCTTCATGC ACCAGGAGTG TGAAGCCATT GTCCAGTCTA TCATTCATAT CCGGACCCGC
TGGGAACTGT CACAGCCCGA CTCTATCCCC CAACACACCA AGATTCGGCC AAAAGATGTC
CCTGGGACAC TGCTCAATAT CGCATTACTT AATTTAGGCA GTTCTGACCC GAGTTTACGG
TCAGCTGCCT ATAATCTTCT GTGTGCCTTA ACTTGTACCT TTAATTTAAA AATCGAGGGC
CAGTTACTAG AGACATCAGG TTTATGTATC CCTGCCAACA ACACCCTCTT TATTGTCTCT
ATTAGTAAGA CACTGGCAGC CAATGAGCCA CACCTCACGT TAGAATTTTT GGAAGAGTGT
ATTTCTGGAT TTAGCAAATC TAGTATTGAA TTGAAACACC TTTGTTTGGA ATACATGACT
CCATGGCTGT CAAATCTAGT TCGTTTTTGC AAGCATAATG ATGATGCCAA ACGACAAAGA
GTTACTGCTA TTCTTGACAA GCTGATAACA ATGACCATCA ATGAAAAACA GATGTACCCA
TCTATTCAAG CAAAAATATG GGGAAGCCTT GGGCAGATTA CAGATCTGCT TGATGTTGTA
CTAGACAGTT TCATCAAAAC CAGTGCAACA GGTGGCTTGG GATCAATAAA AGCTGAGGTG
ATGGCAGATA CTGCTGTAGC TTTGGCTTCT GGAAATGTGA AATTGGTTTC AAGCAAGGTT
ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACATGCTTAT CTCCAACTCC TACTTTAGAA
CAACATCTTA TGTGGGATGA TATTGCTATT TTAGCACGCT ACATGCTGAT GCTGTCCTTC
AACAATTCCC TTGATGTGGC AGCTCATCTT CCCTACCTCT TCCACGTTGT TACTTTCTTA
GTAGCCACAG GTCCGCTCTC CCTTAGAGCT TCCACACATG GACTGGTCAT TAATATCATT
CACTCTCTGT GTACTTGTTC ACAGCTTCAT TTTAGTGAAG AGACCAAGCA AGTTTTGAGA
CTCAGTCTGA CAGAGTTCTC ATTACCCAAA TTTTACTTGC TGTTTGGCAT TAGCAAAGTC
AAGTCAGCTG CTGTCATTGC CTTCCGTTCC AGTTACCGGG ACAGGTCATT CTCTCCTGGC
TCCTATGAGA GAGAGACTTT TGCTTTGACA TCCTTGGAAA CAGTCACAGA AGCTTTGTTG
GAGATCATGG AGGCATGCAT GAGAGATATT CCAACGTGCA AGTGGCTGGA CCAGTGGACA
GAACTAGCTC AAAGATTTGC ATTCCAATAT AATCCATCCC TGCAACCAAG AGCTCTTGTT
GTCTTTGGGT GTATTAGCAA ACGAGTGTCT CATGGGCAGA TAAAGCAGAT AATCCGTATT
CTTAGCAAGG CACTTGAGAG TTGCTTAAAA GGACCTGACA CTTACAACAG TCAAGTTCTG
ATAGAAGCTA CAGTAATAGC ACTAACCAAA TTACAGCCAC TTCTTAATAA GGACTCGCCT
CTGCACAAAG CCCTCTTTTG GGTAGCTGTG GCTGTGCTGC AGCTTGATGA GGTCAACTTG
TATTCAGCAG GTACCGCACT TCTTGAACAA AACCTGCATA CTTTAGATAG TCTCCGTATA
TTCAATGACA AGAGTCCAGA GGAAGTATTT ATGGCAATCC GGAATCCTCT GGAGTGGCAC
TGCAAGCAAA TGGATCATTT TGTTGGACTC AATTTCAACT CTAACTTTAA CTTTGCATTG
GTTGGACACC TTTTAAAAGG GTACAGGCAT CCTTCACCTG CTATTGTTGC AAGAACAGTC
AGAATTTTAC ATACACTACT AACTCTGGTT AACAAACACA GAAATTGTGA CAAATTTGAA
GTGAATACAC AGAGCGTGGC CTACTTAGCA GCTTTACTTA CAGTGTCTGA AGAAGTTCGA
AGTCGCTGCA GCCTAAAACA TAGAAAGTCA CTTCTTCTTA CTGATATTTC AATGGAAAAT
GTTCCTATGG ATACATATCC CATTCATCAT GGTGACCCTT CCTATAGGAC ACTAAAGGAG
ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT GAAGGATACC TTGCAGCCAC CTATCCAACT
GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA TCCATGAGCC TGGACATGGG GCAACCTTCT
CAGGCCAACA CTAAGAAGTT GCTTGGAACA AGGAAAAGTT TTGATCACTT GATATCAGAC
ACAAAGGCTC CTAAAAGGCA AGAAATGGAA TCAGGGATCA CAACACCCCC CAAAATGAGG
AGAGTAGCAG AAACTGATTA TGAAATGGAA ACTCAGAGGA TTTCCTCATC ACAACAGCAC
CCACATTTAC GTAAAGTTTC AGTGTCTGAA TCAAATGTTC TCTTGGATGA AGAAGTACTT
ACTGATCCGA AGATCCAGGC GCTGCTTCTT ACTGTTCTAG CTACACTGGT AAAATATACC
ACAGATGAGT TTGATCAACG AATTCTTTAT GAATACTTAG CAGAGGCCAG TGTTGTGTTT
CCCAAAGTCT TTCCTGTTGT GCATAATTTG TTGGACTCTA AGATCAACAC CCTGTTATCA
TTGTGCCAAG ATCCAAATTT GTTAAATCCA ATCCATGGAA TTGTGCAGAG TGTGGTGTAC
CATGAAGAAT CCCCACCACA ATACCAAACA TCTTACCTGC AAAGTTTTGG TTTTAATGGC
TTGTGGCGGT TTGCAGGACC GTTTTCAAAG CAAACACAAA TTCCAGACTA TGCTGAGCTT
ATTGTTAAGT TTCTTGATGC CTTGATTGAC ACGTACCTGC CTGGAATTGA TGAAGAAACC
AGTGAAGAAT CCCTCCTGAC TCCCACATCT CCTTACCCTC CTGCACTGCA GAGCCAGCTT
AGTATCACTG CCAACCTTAA CCTTTCTAAT TCCATGACCT CACTTGCAAC TTCCCAGCAT
TCCCCAGCTT CTCTGCCTTG CTCTAAATCA GCAGTTTTCA TGCAGCTGTT CCCTCATCAA
GGAATCGACA AGGAGAACGT TGAACTCTCC CCTACCACTG GCCACTGTAA CAGTGGACGA
ACTCGCCACG GATCCGCAAG CCAAGTGCAG AAGCAAAGAA GCGCTGGCAG TTTCAAACGT
AATAGCATTA AGAAGATCGT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59245
Clone ID Related Accession (Same CDS sequence) XM_011524852
Accession Version XM_011524852.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8601bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neurofibromin isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
8581
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
CTTCTTAAAA ATAAGGCAGA TAGAAGTTCC TGTCACTTTC TCCTTTTTTA CGGGGTAGGA
TGTGATATTC CTTCTAGTGG AAATACCAGT CAAATGTCCA TGGATCATGA AGAATTACTA
CGTACTCCTG GAGCCTCTCT CCGGAAGGGA AAAGGGAACT CCTCTATGGA TAGTGCAGCA
GGATGCAGCG GAACCCCCCC GATTTGCCGA CAAGCCCAGA CCAAACTAGA AGTGGCCCTG
TACATGTTTC TGTGGAACCC TGACACTGAA GCTGTTCTGG TTGCCATGTC CTGTTTCCGC
CACCTCTGTG AGGAAGCAGA TATCCGGTGT GGGGTGGATG AAGTGTCAGT GCATAACCTC
TTGCCCAACT ATAACACATT CATGGAGTTT GCCTCTGTCA GCAATATGAT GTCAACAGGA
AGAGCAGCAC TTCAGAAAAG AGTGATGGCA CTGCTGAGGC GCATTGAGCA TCCCACTGCA
GGAAACACTG AGGCTTGGGA AGATACACAT GCAAAATGGG AACAAGCAAC AAAGCTAATC
CTTAACTATC CAAAAGCCAA AATGGAAGAT GGCCAGGCTG CTGAAAGCCT TCACAAGACC
ATTGTTAAGA GGCGAATGTC CCATGTGAGT GGAGGAGGAT CCATAGATTT GTCTGACACA
GACTCCCTAC AGGAATGGAT CAACATGACT GGCTTCCTTT GTGCCCTTGG GGGAGTGTGC
CTCCAGCAGA GAAGCAATTC TGGCCTGGCA ACCTATAGCC CACCCATGGG TCCAGTCAGT
GAACGTAAGG GTTCTATGAT TTCAGTGATG TCTTCAGAGG GAAACGCAGA TACACCTGTC
AGCAAATTTA TGGATCGGCT GTTGTCCTTA ATGGTGTGTA ACCATGAGAA AGTGGGACTT
CAAATACGGA CCAATGTTAA GGATCTGGTG GGTCTAGAAT TGAGTCCTGC TCTGTATCCA
ATGCTATTTA ACAAATTGAA GAATACCATC AGCAAGTTTT TTGACTCCCA AGGACAGGTT
TTATTGACTG ATACCAATAC TCAATTTGTA GAACAAACCA TAGCTATAAT GAAGAACTTG
CTAGATAATC ATACTGAAGG CAGCTCTGAA CATCTAGGGC AAGCTAGCAT TGAAACAATG
ATGTTAAATC TGGTCAGGTA TGTTCGTGTG CTTGGGAATA TGGTCCATGC AATTCAAATA
AAAACGAAAC TGTGTCAATT AGTTGAAGTA ATGATGGCAA GGAGAGATGA CCTCTCATTT
TGCCAAGAGA TGAAATTTAG GAATAAGATG GTAGAATACC TGACAGACTG GGTTATGGGA
ACATCAAACC AAGCAGCAGA TGATGATGTA AAATGTCTTA CAAGAGATTT GGACCAGGCA
AGCATGGAAG CAGTAGTTTC ACTTCTAGCT GGTCTCCCTC TGCAGCCTGA AGAAGGAGAT
GGTGTGGAAT TGATGGAAGC CAAATCACAG TTATTTCTTA AATACTTCAC ATTATTTATG
AACCTTTTGA ATGACTGCAG TGAAGTTGAA GATGAAAGTG CGCAAACAGG TGGCAGGAAA
CGTGGCATGT CTCGGAGGCT GGCATCACTG AGGCACTGTA CGGTCCTTGC AATGTCAAAC
TTACTCAATG CCAACGTAGA CAGTGGTCTC ATGCACTCCA TAGGCTTAGG TTACCACAAG
GATCTCCAGA CAAGAGCTAC ATTTATGGAA GTTCTGACAA AAATCCTTCA ACAAGGCACA
GAATTTGACA CACTTGCAGA AACAGTATTG GCTGATCGGT TTGAGAGATT GGTGGAACTG
GTCACAATGA TGGGTGATCA AGGAGAACTC CCTATAGCGA TGGCTCTGGC CAATGTGGTT
CCTTGTTCTC AGTGGGATGA ACTAGCTCGA GTTCTGGTTA CTCTGTTTGA TTCTCGGCAT
TTACTCTACC AACTGCTCTG GAACATGTTT TCTAAAGAAG TAGAATTGGC AGACTCCATG
CAGACTCTCT TCCGAGGCAA CAGCTTGGCC AGTAAAATAA TGACATTCTG TTTCAAGGTA
TATGGTGCTA CCTATCTACA AAAACTCCTG GATCCTTTAT TACGAATTGT GATCACATCC
TCTGATTGGC AACATGTTAG CTTTGAAGTG GATCCTACCA GGTTAGAACC ATCAGAGAGC
CTTGAGGAAA ACCAGCGGAA CCTCCTTCAG ATGACTGAAA AGTTCTTCCA TGCCATCATC
AGTTCCTCCT CAGAATTCCC CCCTCAACTT CGAAGTGTGT GCCACTGTTT ATACCAGGCA
ACTTGCCACT CCCTACTGAA TAAAGCTACA GTAAAAGAAA AAAAGGAAAA CAAAAAATCA
GTGGTTAGCC AGCGTTTCCC TCAGAACAGC ATCGGTGCAG TAGGAAGTGC CATGTTCCTC
AGATTTATCA ATCCTGCCAT TGTCTCACCG TATGAAGCAG GGATTTTAGA TAAAAAGCCA
CCACCTAGAA TCGAAAGGGG CTTGAAGTTA ATGTCAAAGA TACTTCAGAG TATTGCCAAT
CATGTTCTCT TCACAAAAGA AGAACATATG CGGCCTTTCA ATGATTTTGT GAAAAGCAAC
TTTGATGCAG CACGCAGGTT TTTCCTTGAT ATAGCATCTG ATTGTCCTAC AAGTGATGCA
GTAAATCATA GTCTTTCCTT CATAAGTGAC GGCAATGTGC TTGCTTTACA TCGTCTACTC
TGGAACAATC AGGAGAAAAT TGGGCAGTAT CTTTCCAGCA ACAGGGATCA TAAAGCTGTT
GGAAGACGAC CTTTTGATAA GATGGCAACA CTTCTTGCAT ACCTGGGTCC TCCAGAGCAC
AAACCTGTGG CAGATACACA CTGGTCCAGC CTTAACCTTA CCAGTTCAAA GTTTGAGGAA
TTTATGACTA GGCATCAGGT ACATGAAAAA GAAGAATTCA AGGCTTTGAA AACGTTAAGT
ATTTTCTACC AAGCTGGGAC TTCCAAAGCT GGGAATCCTA TTTTTTATTA TGTTGCACGG
AGGTTCAAAA CTGGTCAAAT CAATGGTGAT TTGCTGATAT ACCATGTCTT ACTGACTTTA
AAGCCATATT ATGCAAAGCC ATATGAAATT GTAGTGGACC TTACCCATAC CGGGCCTAGC
AATCGCTTTA AAACAGACTT TCTCTCTAAG TGGTTTGTTG TTTTTCCTGG CTTTGCTTAC
GACAACGTCT CCGCAGTCTA TATCTATAAC TGTAACTCCT GGGTCAGGGA GTACACCAAG
TATCATGAGC GGCTGCTGAC TGGCCTCAAA GGTAGCAAAA GGCTTGTTTT CATAGACTGT
CCTGGGAAAC TGGCTGAGCA CATAGAGCAT GAACAACAGA AACTACCTGC TGCCACCTTG
GCTTTAGAAG AGGACCTGAA GGTATTCCAC AATGCTCTCA AGCTAGCTCA CAAAGACACC
AAAGTTTCTA TTAAAGTTGG TTCTACTGCT GTCCAAGTAA CTTCAGCAGA GCGAACAAAA
GTCCTAGGGC AATCAGTCTT TCTAAATGAC ATTTATTATG CTTCGGAAAT TGAAGAAATC
TGCCTAGTAG ATGAGAACCA GTTCACCTTA ACCATTGCAA ACCAGGGCAC GCCGCTCACC
TTCATGCACC AGGAGTGTGA AGCCATTGTC CAGTCTATCA TTCATATCCG GACCCGCTGG
GAACTGTCAC AGCCCGACTC TATCCCCCAA CACACCAAGA TTCGGCCAAA AGATGTCCCT
GGGACACTGC TCAATATCGC ATTACTTAAT TTAGGCAGTT CTGACCCGAG TTTACGGTCA
GCTGCCTATA ATCTTCTGTG TGCCTTAACT TGTACCTTTA ATTTAAAAAT CGAGGGCCAG
TTACTAGAGA CATCAGGTTT ATGTATCCCT GCCAACAACA CCCTCTTTAT TGTCTCTATT
AGTAAGACAC TGGCAGCCAA TGAGCCACAC CTCACGTTAG AATTTTTGGA AGAGTGTATT
TCTGGATTTA GCAAATCTAG TATTGAATTG AAACACCTTT GTTTGGAATA CATGACTCCA
TGGCTGTCAA ATCTAGTTCG TTTTTGCAAG CATAATGATG ATGCCAAACG ACAAAGAGTT
ACTGCTATTC TTGACAAGCT GATAACAATG ACCATCAATG AAAAACAGAT GTACCCATCT
ATTCAAGCAA AAATATGGGG AAGCCTTGGG CAGATTACAG ATCTGCTTGA TGTTGTACTA
GACAGTTTCA TCAAAACCAG TGCAACAGGT GGCTTGGGAT CAATAAAAGC TGAGGTGATG
GCAGATACTG CTGTAGCTTT GGCTTCTGGA AATGTGAAAT TGGTTTCAAG CAAGGTTATT
GGAAGGATGT GCAAAATAAT TGACAAGACA TGCTTATCTC CAACTCCTAC TTTAGAACAA
CATCTTATGT GGGATGATAT TGCTATTTTA GCACGCTACA TGCTGATGCT GTCCTTCAAC
AATTCCCTTG ATGTGGCAGC TCATCTTCCC TACCTCTTCC ACGTTGTTAC TTTCTTAGTA
GCCACAGGTC CGCTCTCCCT TAGAGCTTCC ACACATGGAC TGGTCATTAA TATCATTCAC
TCTCTGTGTA CTTGTTCACA GCTTCATTTT AGTGAAGAGA CCAAGCAAGT TTTGAGACTC
AGTCTGACAG AGTTCTCATT ACCCAAATTT TACTTGCTGT TTGGCATTAG CAAAGTCAAG
TCAGCTGCTG TCATTGCCTT CCGTTCCAGT TACCGGGACA GGTCATTCTC TCCTGGCTCC
TATGAGAGAG AGACTTTTGC TTTGACATCC TTGGAAACAG TCACAGAAGC TTTGTTGGAG
ATCATGGAGG CATGCATGAG AGATATTCCA ACGTGCAAGT GGCTGGACCA GTGGACAGAA
CTAGCTCAAA GATTTGCATT CCAATATAAT CCATCCCTGC AACCAAGAGC TCTTGTTGTC
TTTGGGTGTA TTAGCAAACG AGTGTCTCAT GGGCAGATAA AGCAGATAAT CCGTATTCTT
AGCAAGGCAC TTGAGAGTTG CTTAAAAGGA CCTGACACTT ACAACAGTCA AGTTCTGATA
GAAGCTACAG TAATAGCACT AACCAAATTA CAGCCACTTC TTAATAAGGA CTCGCCTCTG
CACAAAGCCC TCTTTTGGGT AGCTGTGGCT GTGCTGCAGC TTGATGAGGT CAACTTGTAT
TCAGCAGGTA CCGCACTTCT TGAACAAAAC CTGCATACTT TAGATAGTCT CCGTATATTC
AATGACAAGA GTCCAGAGGA AGTATTTATG GCAATCCGGA ATCCTCTGGA GTGGCACTGC
AAGCAAATGG ATCATTTTGT TGGACTCAAT TTCAACTCTA ACTTTAACTT TGCATTGGTT
GGACACCTTT TAAAAGGGTA CAGGCATCCT TCACCTGCTA TTGTTGCAAG AACAGTCAGA
ATTTTACATA CACTACTAAC TCTGGTTAAC AAACACAGAA ATTGTGACAA ATTTGAAGTG
AATACACAGA GCGTGGCCTA CTTAGCAGCT TTACTTACAG TGTCTGAAGA AGTTCGAAGT
CGCTGCAGCC TAAAACATAG AAAGTCACTT CTTCTTACTG ATATTTCAAT GGAAAATGTT
CCTATGGATA CATATCCCAT TCATCATGGT GACCCTTCCT ATAGGACACT AAAGGAGACT
CAGCCATGGT CCTCTCCCAA AGGTTCTGAA GGATACCTTG CAGCCACCTA TCCAACTGTC
GGCCAGACCA GTCCCCGAGC CAGGAAATCC ATGAGCCTGG ACATGGGGCA ACCTTCTCAG
GCCAACACTA AGAAGTTGCT TGGAACAAGG AAAAGTTTTG ATCACTTGAT ATCAGACACA
AAGGCTCCTA AAAGGCAAGA AATGGAATCA GGGATCACAA CACCCCCCAA AATGAGGAGA
GTAGCAGAAA CTGATTATGA AATGGAAACT CAGAGGATTT CCTCATCACA ACAGCACCCA
CATTTACGTA AAGTTTCAGT GTCTGAATCA AATGTTCTCT TGGATGAAGA AGTACTTACT
GATCCGAAGA TCCAGGCGCT GCTTCTTACT GTTCTAGCTA CACTGGTAAA ATATACCACA
GATGAGTTTG ATCAACGAAT TCTTTATGAA TACTTAGCAG AGGCCAGTGT TGTGTTTCCC
AAAGTCTTTC CTGTTGTGCA TAATTTGTTG GACTCTAAGA TCAACACCCT GTTATCATTG
TGCCAAGATC CAAATTTGTT AAATCCAATC CATGGAATTG TGCAGAGTGT GGTGTACCAT
GAAGAATCCC CACCACAATA CCAAACATCT TACCTGCAAA GTTTTGGTTT TAATGGCTTG
TGGCGGTTTG CAGGACCGTT TTCAAAGCAA ACACAAATTC CAGACTATGC TGAGCTTATT
GTTAAGTTTC TTGATGCCTT GATTGACACG TACCTGCCTG GAATTGATGA AGAAACCAGT
GAAGAATCCC TCCTGACTCC CACATCTCCT TACCCTCCTG CACTGCAGAG CCAGCTTAGT
ATCACTGCCA ACCTTAACCT TTCTAATTCC ATGACCTCAC TTGCAACTTC CCAGCATTCC
CCAGCTTCTC TGCCTTGCTC TAAATCAGCA GTTTTCATGC AGCTGTTCCC TCATCAAGGA
ATCGACAAGG AGAACGTTGA ACTCTCCCCT ACCACTGGCC ACTGTAACAG TGGACGAACT
CGCCACGGAT CCGCAAGCCA AGTGCAGAAG CAAAGAAGCG CTGGCAGTTT CAAACGTAAT
AGCATTAAGA AGATCGTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523154.1
CDS334..8934
Misc Feature(1)3919..5031(+)
Misc Feature(2)3952..5013(+)
Misc Feature(3)4066..4734(+)
Misc Feature(4)5101..5544(+)
Misc Feature(5)5170..5475(+)
Misc Feature(6)5374..5472(+)
Misc Feature(7)5542..5871(+)
Misc Feature(8)5542..5871(+)
Translation

Target ORF information:

RefSeq Version XM_011524852
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524852

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
8581
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
CTTCTTAAAA ATAAGGCAGA TAGAAGTTCC TGTCACTTTC TCCTTTTTTA CGGGGTAGGA
TGTGATATTC CTTCTAGTGG AAATACCAGT CAAATGTCCA TGGATCATGA AGAATTACTA
CGTACTCCTG GAGCCTCTCT CCGGAAGGGA AAAGGGAACT CCTCTATGGA TAGTGCAGCA
GGATGCAGCG GAACCCCCCC GATTTGCCGA CAAGCCCAGA CCAAACTAGA AGTGGCCCTG
TACATGTTTC TGTGGAACCC TGACACTGAA GCTGTTCTGG TTGCCATGTC CTGTTTCCGC
CACCTCTGTG AGGAAGCAGA TATCCGGTGT GGGGTGGATG AAGTGTCAGT GCATAACCTC
TTGCCCAACT ATAACACATT CATGGAGTTT GCCTCTGTCA GCAATATGAT GTCAACAGGA
AGAGCAGCAC TTCAGAAAAG AGTGATGGCA CTGCTGAGGC GCATTGAGCA TCCCACTGCA
GGAAACACTG AGGCTTGGGA AGATACACAT GCAAAATGGG AACAAGCAAC AAAGCTAATC
CTTAACTATC CAAAAGCCAA AATGGAAGAT GGCCAGGCTG CTGAAAGCCT TCACAAGACC
ATTGTTAAGA GGCGAATGTC CCATGTGAGT GGAGGAGGAT CCATAGATTT GTCTGACACA
GACTCCCTAC AGGAATGGAT CAACATGACT GGCTTCCTTT GTGCCCTTGG GGGAGTGTGC
CTCCAGCAGA GAAGCAATTC TGGCCTGGCA ACCTATAGCC CACCCATGGG TCCAGTCAGT
GAACGTAAGG GTTCTATGAT TTCAGTGATG TCTTCAGAGG GAAACGCAGA TACACCTGTC
AGCAAATTTA TGGATCGGCT GTTGTCCTTA ATGGTGTGTA ACCATGAGAA AGTGGGACTT
CAAATACGGA CCAATGTTAA GGATCTGGTG GGTCTAGAAT TGAGTCCTGC TCTGTATCCA
ATGCTATTTA ACAAATTGAA GAATACCATC AGCAAGTTTT TTGACTCCCA AGGACAGGTT
TTATTGACTG ATACCAATAC TCAATTTGTA GAACAAACCA TAGCTATAAT GAAGAACTTG
CTAGATAATC ATACTGAAGG CAGCTCTGAA CATCTAGGGC AAGCTAGCAT TGAAACAATG
ATGTTAAATC TGGTCAGGTA TGTTCGTGTG CTTGGGAATA TGGTCCATGC AATTCAAATA
AAAACGAAAC TGTGTCAATT AGTTGAAGTA ATGATGGCAA GGAGAGATGA CCTCTCATTT
TGCCAAGAGA TGAAATTTAG GAATAAGATG GTAGAATACC TGACAGACTG GGTTATGGGA
ACATCAAACC AAGCAGCAGA TGATGATGTA AAATGTCTTA CAAGAGATTT GGACCAGGCA
AGCATGGAAG CAGTAGTTTC ACTTCTAGCT GGTCTCCCTC TGCAGCCTGA AGAAGGAGAT
GGTGTGGAAT TGATGGAAGC CAAATCACAG TTATTTCTTA AATACTTCAC ATTATTTATG
AACCTTTTGA ATGACTGCAG TGAAGTTGAA GATGAAAGTG CGCAAACAGG TGGCAGGAAA
CGTGGCATGT CTCGGAGGCT GGCATCACTG AGGCACTGTA CGGTCCTTGC AATGTCAAAC
TTACTCAATG CCAACGTAGA CAGTGGTCTC ATGCACTCCA TAGGCTTAGG TTACCACAAG
GATCTCCAGA CAAGAGCTAC ATTTATGGAA GTTCTGACAA AAATCCTTCA ACAAGGCACA
GAATTTGACA CACTTGCAGA AACAGTATTG GCTGATCGGT TTGAGAGATT GGTGGAACTG
GTCACAATGA TGGGTGATCA AGGAGAACTC CCTATAGCGA TGGCTCTGGC CAATGTGGTT
CCTTGTTCTC AGTGGGATGA ACTAGCTCGA GTTCTGGTTA CTCTGTTTGA TTCTCGGCAT
TTACTCTACC AACTGCTCTG GAACATGTTT TCTAAAGAAG TAGAATTGGC AGACTCCATG
CAGACTCTCT TCCGAGGCAA CAGCTTGGCC AGTAAAATAA TGACATTCTG TTTCAAGGTA
TATGGTGCTA CCTATCTACA AAAACTCCTG GATCCTTTAT TACGAATTGT GATCACATCC
TCTGATTGGC AACATGTTAG CTTTGAAGTG GATCCTACCA GGTTAGAACC ATCAGAGAGC
CTTGAGGAAA ACCAGCGGAA CCTCCTTCAG ATGACTGAAA AGTTCTTCCA TGCCATCATC
AGTTCCTCCT CAGAATTCCC CCCTCAACTT CGAAGTGTGT GCCACTGTTT ATACCAGGCA
ACTTGCCACT CCCTACTGAA TAAAGCTACA GTAAAAGAAA AAAAGGAAAA CAAAAAATCA
GTGGTTAGCC AGCGTTTCCC TCAGAACAGC ATCGGTGCAG TAGGAAGTGC CATGTTCCTC
AGATTTATCA ATCCTGCCAT TGTCTCACCG TATGAAGCAG GGATTTTAGA TAAAAAGCCA
CCACCTAGAA TCGAAAGGGG CTTGAAGTTA ATGTCAAAGA TACTTCAGAG TATTGCCAAT
CATGTTCTCT TCACAAAAGA AGAACATATG CGGCCTTTCA ATGATTTTGT GAAAAGCAAC
TTTGATGCAG CACGCAGGTT TTTCCTTGAT ATAGCATCTG ATTGTCCTAC AAGTGATGCA
GTAAATCATA GTCTTTCCTT CATAAGTGAC GGCAATGTGC TTGCTTTACA TCGTCTACTC
TGGAACAATC AGGAGAAAAT TGGGCAGTAT CTTTCCAGCA ACAGGGATCA TAAAGCTGTT
GGAAGACGAC CTTTTGATAA GATGGCAACA CTTCTTGCAT ACCTGGGTCC TCCAGAGCAC
AAACCTGTGG CAGATACACA CTGGTCCAGC CTTAACCTTA CCAGTTCAAA GTTTGAGGAA
TTTATGACTA GGCATCAGGT ACATGAAAAA GAAGAATTCA AGGCTTTGAA AACGTTAAGT
ATTTTCTACC AAGCTGGGAC TTCCAAAGCT GGGAATCCTA TTTTTTATTA TGTTGCACGG
AGGTTCAAAA CTGGTCAAAT CAATGGTGAT TTGCTGATAT ACCATGTCTT ACTGACTTTA
AAGCCATATT ATGCAAAGCC ATATGAAATT GTAGTGGACC TTACCCATAC CGGGCCTAGC
AATCGCTTTA AAACAGACTT TCTCTCTAAG TGGTTTGTTG TTTTTCCTGG CTTTGCTTAC
GACAACGTCT CCGCAGTCTA TATCTATAAC TGTAACTCCT GGGTCAGGGA GTACACCAAG
TATCATGAGC GGCTGCTGAC TGGCCTCAAA GGTAGCAAAA GGCTTGTTTT CATAGACTGT
CCTGGGAAAC TGGCTGAGCA CATAGAGCAT GAACAACAGA AACTACCTGC TGCCACCTTG
GCTTTAGAAG AGGACCTGAA GGTATTCCAC AATGCTCTCA AGCTAGCTCA CAAAGACACC
AAAGTTTCTA TTAAAGTTGG TTCTACTGCT GTCCAAGTAA CTTCAGCAGA GCGAACAAAA
GTCCTAGGGC AATCAGTCTT TCTAAATGAC ATTTATTATG CTTCGGAAAT TGAAGAAATC
TGCCTAGTAG ATGAGAACCA GTTCACCTTA ACCATTGCAA ACCAGGGCAC GCCGCTCACC
TTCATGCACC AGGAGTGTGA AGCCATTGTC CAGTCTATCA TTCATATCCG GACCCGCTGG
GAACTGTCAC AGCCCGACTC TATCCCCCAA CACACCAAGA TTCGGCCAAA AGATGTCCCT
GGGACACTGC TCAATATCGC ATTACTTAAT TTAGGCAGTT CTGACCCGAG TTTACGGTCA
GCTGCCTATA ATCTTCTGTG TGCCTTAACT TGTACCTTTA ATTTAAAAAT CGAGGGCCAG
TTACTAGAGA CATCAGGTTT ATGTATCCCT GCCAACAACA CCCTCTTTAT TGTCTCTATT
AGTAAGACAC TGGCAGCCAA TGAGCCACAC CTCACGTTAG AATTTTTGGA AGAGTGTATT
TCTGGATTTA GCAAATCTAG TATTGAATTG AAACACCTTT GTTTGGAATA CATGACTCCA
TGGCTGTCAA ATCTAGTTCG TTTTTGCAAG CATAATGATG ATGCCAAACG ACAAAGAGTT
ACTGCTATTC TTGACAAGCT GATAACAATG ACCATCAATG AAAAACAGAT GTACCCATCT
ATTCAAGCAA AAATATGGGG AAGCCTTGGG CAGATTACAG ATCTGCTTGA TGTTGTACTA
GACAGTTTCA TCAAAACCAG TGCAACAGGT GGCTTGGGAT CAATAAAAGC TGAGGTGATG
GCAGATACTG CTGTAGCTTT GGCTTCTGGA AATGTGAAAT TGGTTTCAAG CAAGGTTATT
GGAAGGATGT GCAAAATAAT TGACAAGACA TGCTTATCTC CAACTCCTAC TTTAGAACAA
CATCTTATGT GGGATGATAT TGCTATTTTA GCACGCTACA TGCTGATGCT GTCCTTCAAC
AATTCCCTTG ATGTGGCAGC TCATCTTCCC TACCTCTTCC ACGTTGTTAC TTTCTTAGTA
GCCACAGGTC CGCTCTCCCT TAGAGCTTCC ACACATGGAC TGGTCATTAA TATCATTCAC
TCTCTGTGTA CTTGTTCACA GCTTCATTTT AGTGAAGAGA CCAAGCAAGT TTTGAGACTC
AGTCTGACAG AGTTCTCATT ACCCAAATTT TACTTGCTGT TTGGCATTAG CAAAGTCAAG
TCAGCTGCTG TCATTGCCTT CCGTTCCAGT TACCGGGACA GGTCATTCTC TCCTGGCTCC
TATGAGAGAG AGACTTTTGC TTTGACATCC TTGGAAACAG TCACAGAAGC TTTGTTGGAG
ATCATGGAGG CATGCATGAG AGATATTCCA ACGTGCAAGT GGCTGGACCA GTGGACAGAA
CTAGCTCAAA GATTTGCATT CCAATATAAT CCATCCCTGC AACCAAGAGC TCTTGTTGTC
TTTGGGTGTA TTAGCAAACG AGTGTCTCAT GGGCAGATAA AGCAGATAAT CCGTATTCTT
AGCAAGGCAC TTGAGAGTTG CTTAAAAGGA CCTGACACTT ACAACAGTCA AGTTCTGATA
GAAGCTACAG TAATAGCACT AACCAAATTA CAGCCACTTC TTAATAAGGA CTCGCCTCTG
CACAAAGCCC TCTTTTGGGT AGCTGTGGCT GTGCTGCAGC TTGATGAGGT CAACTTGTAT
TCAGCAGGTA CCGCACTTCT TGAACAAAAC CTGCATACTT TAGATAGTCT CCGTATATTC
AATGACAAGA GTCCAGAGGA AGTATTTATG GCAATCCGGA ATCCTCTGGA GTGGCACTGC
AAGCAAATGG ATCATTTTGT TGGACTCAAT TTCAACTCTA ACTTTAACTT TGCATTGGTT
GGACACCTTT TAAAAGGGTA CAGGCATCCT TCACCTGCTA TTGTTGCAAG AACAGTCAGA
ATTTTACATA CACTACTAAC TCTGGTTAAC AAACACAGAA ATTGTGACAA ATTTGAAGTG
AATACACAGA GCGTGGCCTA CTTAGCAGCT TTACTTACAG TGTCTGAAGA AGTTCGAAGT
CGCTGCAGCC TAAAACATAG AAAGTCACTT CTTCTTACTG ATATTTCAAT GGAAAATGTT
CCTATGGATA CATATCCCAT TCATCATGGT GACCCTTCCT ATAGGACACT AAAGGAGACT
CAGCCATGGT CCTCTCCCAA AGGTTCTGAA GGATACCTTG CAGCCACCTA TCCAACTGTC
GGCCAGACCA GTCCCCGAGC CAGGAAATCC ATGAGCCTGG ACATGGGGCA ACCTTCTCAG
GCCAACACTA AGAAGTTGCT TGGAACAAGG AAAAGTTTTG ATCACTTGAT ATCAGACACA
AAGGCTCCTA AAAGGCAAGA AATGGAATCA GGGATCACAA CACCCCCCAA AATGAGGAGA
GTAGCAGAAA CTGATTATGA AATGGAAACT CAGAGGATTT CCTCATCACA ACAGCACCCA
CATTTACGTA AAGTTTCAGT GTCTGAATCA AATGTTCTCT TGGATGAAGA AGTACTTACT
GATCCGAAGA TCCAGGCGCT GCTTCTTACT GTTCTAGCTA CACTGGTAAA ATATACCACA
GATGAGTTTG ATCAACGAAT TCTTTATGAA TACTTAGCAG AGGCCAGTGT TGTGTTTCCC
AAAGTCTTTC CTGTTGTGCA TAATTTGTTG GACTCTAAGA TCAACACCCT GTTATCATTG
TGCCAAGATC CAAATTTGTT AAATCCAATC CATGGAATTG TGCAGAGTGT GGTGTACCAT
GAAGAATCCC CACCACAATA CCAAACATCT TACCTGCAAA GTTTTGGTTT TAATGGCTTG
TGGCGGTTTG CAGGACCGTT TTCAAAGCAA ACACAAATTC CAGACTATGC TGAGCTTATT
GTTAAGTTTC TTGATGCCTT GATTGACACG TACCTGCCTG GAATTGATGA AGAAACCAGT
GAAGAATCCC TCCTGACTCC CACATCTCCT TACCCTCCTG CACTGCAGAG CCAGCTTAGT
ATCACTGCCA ACCTTAACCT TTCTAATTCC ATGACCTCAC TTGCAACTTC CCAGCATTCC
CCAGCTTCTC TGCCTTGCTC TAAATCAGCA GTTTTCATGC AGCTGTTCCC TCATCAAGGA
ATCGACAAGG AGAACGTTGA ACTCTCCCCT ACCACTGGCC ACTGTAACAG TGGACGAACT
CGCCACGGAT CCGCAAGCCA AGTGCAGAAG CAAAGAAGCG CTGGCAGTTT CAAACGTAAT
AGCATTAAGA AGATCGTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50428
Clone ID Related Accession (Same CDS sequence) XM_005257983
Accession Version XM_005257983.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8574bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neurofibromin isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GCTCACAAAT GCTTTTTTAC
ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTG
CGGGAAATAT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
TCCTGTCACT TTCTCCTTTT TTACGGGGTA GGATGTGATA TTCCTTCTAG TGGAAATACC
AGTCAAATGT CCATGGATCA TGAAGAATTA CTACGTACTC CTGGAGCCTC TCTCCGGAAG
GGAAAAGGGA ACTCCTCTAT GGATAGTGCA GCAGGATGCA GCGGAACCCC CCCGATTTGC
CGACAAGCCC AGACCAAACT AGAAGTGGCC CTGTACATGT TTCTGTGGAA CCCTGACACT
GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC CGCCACCTCT GTGAGGAAGC AGATATCCGG
TGTGGGGTGG ATGAAGTGTC AGTGCATAAC CTCTTGCCCA ACTATAACAC ATTCATGGAG
TTTGCCTCTG TCAGCAATAT GATGTCAACA GGAAGAGCAG CACTTCAGAA AAGAGTGATG
GCACTGCTGA GGCGCATTGA GCATCCCACT GCAGGAAACA CTGAGGCTTG GGAAGATACA
CATGCAAAAT GGGAACAAGC AACAAAGCTA ATCCTTAACT ATCCAAAAGC CAAAATGGAA
GATGGCCAGG CTGCTGAAAG CCTTCACAAG ACCATTGTTA AGAGGCGAAT GTCCCATGTG
AGTGGAGGAG GATCCATAGA TTTGTCTGAC ACAGACTCCC TACAGGAATG GATCAACATG
ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG
GCAACCTATA GCCCACCCAT GGGTCCAGTC AGTGAACGTA AGGGTTCTAT GATTTCAGTG
ATGTCTTCAG AGGGAAACGC AGATACACCT GTCAGCAAAT TTATGGATCG GCTGTTGTCC
TTAATGGTGT GTAACCATGA GAAAGTGGGA CTTCAAATAC GGACCAATGT TAAGGATCTG
GTGGGTCTAG AATTGAGTCC TGCTCTGTAT CCAATGCTAT TTAACAAATT GAAGAATACC
ATCAGCAAGT TTTTTGACTC CCAAGGACAG GTTTTATTGA CTGATACCAA TACTCAATTT
GTAGAACAAA CCATAGCTAT AATGAAGAAC TTGCTAGATA ATCATACTGA AGGCAGCTCT
GAACATCTAG GGCAAGCTAG CATTGAAACA ATGATGTTAA ATCTGGTCAG GTATGTTCGT
GTGCTTGGGA ATATGGTCCA TGCAATTCAA ATAAAAACGA AACTGTGTCA ATTAGTTGAA
GTAATGATGG CAAGGAGAGA TGACCTCTCA TTTTGCCAAG AGATGAAATT TAGGAATAAG
ATGGTAGAAT ACCTGACAGA CTGGGTTATG GGAACATCAA ACCAAGCAGC AGATGATGAT
GTAAAATGTC TTACAAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTAGT TTCACTTCTA
GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA GATGGTGTGG AATTGATGGA AGCCAAATCA
CAGTTATTTC TTAAATACTT CACATTATTT ATGAACCTTT TGAATGACTG CAGTGAAGTT
GAAGATGAAA GTGCGCAAAC AGGTGGCAGG AAACGTGGCA TGTCTCGGAG GCTGGCATCA
CTGAGGCACT GTACGGTCCT TGCAATGTCA AACTTACTCA ATGCCAACGT AGACAGTGGT
CTCATGCACT CCATAGGCTT AGGTTACCAC AAGGATCTCC AGACAAGAGC TACATTTATG
GAAGTTCTGA CAAAAATCCT TCAACAAGGC ACAGAATTTG ACACACTTGC AGAAACAGTA
TTGGCTGATC GGTTTGAGAG ATTGGTGGAA CTGGTCACAA TGATGGGTGA TCAAGGAGAA
CTCCCTATAG CGATGGCTCT GGCCAATGTG GTTCCTTGTT CTCAGTGGGA TGAACTAGCT
CGAGTTCTGG TTACTCTGTT TGATTCTCGG CATTTACTCT ACCAACTGCT CTGGAACATG
TTTTCTAAAG AAGTAGAATT GGCAGACTCC ATGCAGACTC TCTTCCGAGG CAACAGCTTG
GCCAGTAAAA TAATGACATT CTGTTTCAAG GTATATGGTG CTACCTATCT ACAAAAACTC
CTGGATCCTT TATTACGAAT TGTGATCACA TCCTCTGATT GGCAACATGT TAGCTTTGAA
GTGGATCCTA CCAGGTTAGA ACCATCAGAG AGCCTTGAGG AAAACCAGCG GAACCTCCTT
CAGATGACTG AAAAGTTCTT CCATGCCATC ATCAGTTCCT CCTCAGAATT CCCCCCTCAA
CTTCGAAGTG TGTGCCACTG TTTATACCAG GCAACTTGCC ACTCCCTACT GAATAAAGCT
ACAGTAAAAG AAAAAAAGGA AAACAAAAAA TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC
AGCATCGGTG CAGTAGGAAG TGCCATGTTC CTCAGATTTA TCAATCCTGC CATTGTCTCA
CCGTATGAAG CAGGGATTTT AGATAAAAAG CCACCACCTA GAATCGAAAG GGGCTTGAAG
TTAATGTCAA AGATACTTCA GAGTATTGCC AATCATGTTC TCTTCACAAA AGAAGAACAT
ATGCGGCCTT TCAATGATTT TGTGAAAAGC AACTTTGATG CAGCACGCAG GTTTTTCCTT
GATATAGCAT CTGATTGTCC TACAAGTGAT GCAGTAAATC ATAGTCTTTC CTTCATAAGT
GACGGCAATG TGCTTGCTTT ACATCGTCTA CTCTGGAACA ATCAGGAGAA AATTGGGCAG
TATCTTTCCA GCAACAGGGA TCATAAAGCT GTTGGAAGAC GACCTTTTGA TAAGATGGCA
ACACTTCTTG CATACCTGGG TCCTCCAGAG CACAAACCTG TGGCAGATAC ACACTGGTCC
AGCCTTAACC TTACCAGTTC AAAGTTTGAG GAATTTATGA CTAGGCATCA GGTACATGAA
AAAGAAGAAT TCAAGGCTTT GAAAACGTTA AGTATTTTCT ACCAAGCTGG GACTTCCAAA
GCTGGGAATC CTATTTTTTA TTATGTTGCA CGGAGGTTCA AAACTGGTCA AATCAATGGT
GATTTGCTGA TATACCATGT CTTACTGACT TTAAAGCCAT ATTATGCAAA GCCATATGAA
ATTGTAGTGG ACCTTACCCA TACCGGGCCT AGCAATCGCT TTAAAACAGA CTTTCTCTCT
AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT TACGACAACG TCTCCGCAGT CTATATCTAT
AACTGTAACT CCTGGGTCAG GGAGTACACC AAGTATCATG AGCGGCTGCT GACTGGCCTC
AAAGGTAGCA AAAGGCTTGT TTTCATAGAC TGTCCTGGGA AACTGGCTGA GCACATAGAG
CATGAACAAC AGAAACTACC TGCTGCCACC TTGGCTTTAG AAGAGGACCT GAAGGTATTC
CACAATGCTC TCAAGCTAGC TCACAAAGAC ACCAAAGTTT CTATTAAAGT TGGTTCTACT
GCTGTCCAAG TAACTTCAGC AGAGCGAACA AAAGTCCTAG GGCAATCAGT CTTTCTAAAT
GACATTTATT ATGCTTCGGA AATTGAAGAA ATCTGCCTAG TAGATGAGAA CCAGTTCACC
TTAACCATTG CAAACCAGGG CACGCCGCTC ACCTTCATGC ACCAGGAGTG TGAAGCCATT
GTCCAGTCTA TCATTCATAT CCGGACCCGC TGGGAACTGT CACAGCCCGA CTCTATCCCC
CAACACACCA AGATTCGGCC AAAAGATGTC CCTGGGACAC TGCTCAATAT CGCATTACTT
AATTTAGGCA GTTCTGACCC GAGTTTACGG TCAGCTGCCT ATAATCTTCT GTGTGCCTTA
ACTTGTACCT TTAATTTAAA AATCGAGGGC CAGTTACTAG AGACATCAGG TTTATGTATC
CCTGCCAACA ACACCCTCTT TATTGTCTCT ATTAGTAAGA CACTGGCAGC CAATGAGCCA
CACCTCACGT TAGAATTTTT GGAAGAGTGT ATTTCTGGAT TTAGCAAATC TAGTATTGAA
TTGAAACACC TTTGTTTGGA ATACATGACT CCATGGCTGT CAAATCTAGT TCGTTTTTGC
AAGCATAATG ATGATGCCAA ACGACAAAGA GTTACTGCTA TTCTTGACAA GCTGATAACA
ATGACCATCA ATGAAAAACA GATGTACCCA TCTATTCAAG CAAAAATATG GGGAAGCCTT
GGGCAGATTA CAGATCTGCT TGATGTTGTA CTAGACAGTT TCATCAAAAC CAGTGCAACA
GGTGGCTTGG GATCAATAAA AGCTGAGGTG ATGGCAGATA CTGCTGTAGC TTTGGCTTCT
GGAAATGTGA AATTGGTTTC AAGCAAGGTT ATTGGAAGGA TGTGCAAAAT AATTGACAAG
ACATGCTTAT CTCCAACTCC TACTTTAGAA CAACATCTTA TGTGGGATGA TATTGCTATT
TTAGCACGCT ACATGCTGAT GCTGTCCTTC AACAATTCCC TTGATGTGGC AGCTCATCTT
CCCTACCTCT TCCACGTTGT TACTTTCTTA GTAGCCACAG GTCCGCTCTC CCTTAGAGCT
TCCACACATG GACTGGTCAT TAATATCATT CACTCTCTGT GTACTTGTTC ACAGCTTCAT
TTTAGTGAAG AGACCAAGCA AGTTTTGAGA CTCAGTCTGA CAGAGTTCTC ATTACCCAAA
TTTTACTTGC TGTTTGGCAT TAGCAAAGTC AAGTCAGCTG CTGTCATTGC CTTCCGTTCC
AGTTACCGGG ACAGGTCATT CTCTCCTGGC TCCTATGAGA GAGAGACTTT TGCTTTGACA
TCCTTGGAAA CAGTCACAGA AGCTTTGTTG GAGATCATGG AGGCATGCAT GAGAGATATT
CCAACGTGCA AGTGGCTGGA CCAGTGGACA GAACTAGCTC AAAGATTTGC ATTCCAATAT
AATCCATCCC TGCAACCAAG AGCTCTTGTT GTCTTTGGGT GTATTAGCAA ACGAGTGTCT
CATGGGCAGA TAAAGCAGAT AATCCGTATT CTTAGCAAGG CACTTGAGAG TTGCTTAAAA
GGACCTGACA CTTACAACAG TCAAGTTCTG ATAGAAGCTA CAGTAATAGC ACTAACCAAA
TTACAGCCAC TTCTTAATAA GGACTCGCCT CTGCACAAAG CCCTCTTTTG GGTAGCTGTG
GCTGTGCTGC AGCTTGATGA GGTCAACTTG TATTCAGCAG GTACCGCACT TCTTGAACAA
AACCTGCATA CTTTAGATAG TCTCCGTATA TTCAATGACA AGAGTCCAGA GGAAGTATTT
ATGGCAATCC GGAATCCTCT GGAGTGGCAC TGCAAGCAAA TGGATCATTT TGTTGGACTC
AATTTCAACT CTAACTTTAA CTTTGCATTG GTTGGACACC TTTTAAAAGG GTACAGGCAT
CCTTCACCTG CTATTGTTGC AAGAACAGTC AGAATTTTAC ATACACTACT AACTCTGGTT
AACAAACACA GAAATTGTGA CAAATTTGAA GTGAATACAC AGAGCGTGGC CTACTTAGCA
GCTTTACTTA CAGTGTCTGA AGAAGTTCGA AGTCGCTGCA GCCTAAAACA TAGAAAGTCA
CTTCTTCTTA CTGATATTTC AATGGAAAAT GTTCCTATGG ATACATATCC CATTCATCAT
GGTGACCCTT CCTATAGGAC ACTAAAGGAG ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT
GAAGGATACC TTGCAGCCAC CTATCCAACT GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA
TCCATGAGCC TGGACATGGG GCAACCTTCT CAGGCCAACA CTAAGAAGTT GCTTGGAACA
AGGAAAAGTT TTGATCACTT GATATCAGAC ACAAAGGCTC CTAAAAGGCA AGAAATGGAA
TCAGGGATCA CAACACCCCC CAAAATGAGG AGAGTAGCAG AAACTGATTA TGAAATGGAA
ACTCAGAGGA TTTCCTCATC ACAACAGCAC CCACATTTAC GTAAAGTTTC AGTGTCTGAA
TCAAATGTTC TCTTGGATGA AGAAGTACTT ACTGATCCGA AGATCCAGGC GCTGCTTCTT
ACTGTTCTAG CTACACTGGT AAAATATACC ACAGATGAGT TTGATCAACG AATTCTTTAT
GAATACTTAG CAGAGGCCAG TGTTGTGTTT CCCAAAGTCT TTCCTGTTGT GCATAATTTG
TTGGACTCTA AGATCAACAC CCTGTTATCA TTGTGCCAAG ATCCAAATTT GTTAAATCCA
ATCCATGGAA TTGTGCAGAG TGTGGTGTAC CATGAAGAAT CCCCACCACA ATACCAAACA
TCTTACCTGC AAAGTTTTGG TTTTAATGGC TTGTGGCGGT TTGCAGGACC GTTTTCAAAG
CAAACACAAA TTCCAGACTA TGCTGAGCTT ATTGTTAAGT TTCTTGATGC CTTGATTGAC
ACGTACCTGC CTGGAATTGA TGAAGAAACC AGTGAAGAAT CCCTCCTGAC TCCCACATCT
CCTTACCCTC CTGCACTGCA GAGCCAGCTT AGTATCACTG CCAACCTTAA CCTTTCTAAT
TCCATGACCT CACTTGCAAC TTCCCAGCAT TCCCCAGCTT CTCTGCCTTG CTCTAAATCA
GCAGTTTTCA TGCAGCTGTT CCCTCATCAA GGAATCGACA AGGAGAACGT TGAACTCTCC
CCTACCACTG GCCACTGTAA CAGTGGACGA ACTCGCCACG GATCCGCAAG CCAAGTGCAG
AAGCAAAGAA GCGCTGGCAG TTTCAAACGT AATAGCATTA AGAAGATCGT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005258040.1
CDS334..8907
Misc Feature(1)3892..5004(+)
Misc Feature(2)3925..4986(+)
Misc Feature(3)4039..4707(+)
Misc Feature(4)5074..5517(+)
Misc Feature(5)5143..5448(+)
Misc Feature(6)5347..5445(+)
Misc Feature(7)5515..5844(+)
Misc Feature(8)5515..5844(+)
Translation

Target ORF information:

RefSeq Version XM_005257983
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257983

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GCTCACAAAT GCTTTTTTAC
ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTG
CGGGAAATAT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
TCCTGTCACT TTCTCCTTTT TTACGGGGTA GGATGTGATA TTCCTTCTAG TGGAAATACC
AGTCAAATGT CCATGGATCA TGAAGAATTA CTACGTACTC CTGGAGCCTC TCTCCGGAAG
GGAAAAGGGA ACTCCTCTAT GGATAGTGCA GCAGGATGCA GCGGAACCCC CCCGATTTGC
CGACAAGCCC AGACCAAACT AGAAGTGGCC CTGTACATGT TTCTGTGGAA CCCTGACACT
GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC CGCCACCTCT GTGAGGAAGC AGATATCCGG
TGTGGGGTGG ATGAAGTGTC AGTGCATAAC CTCTTGCCCA ACTATAACAC ATTCATGGAG
TTTGCCTCTG TCAGCAATAT GATGTCAACA GGAAGAGCAG CACTTCAGAA AAGAGTGATG
GCACTGCTGA GGCGCATTGA GCATCCCACT GCAGGAAACA CTGAGGCTTG GGAAGATACA
CATGCAAAAT GGGAACAAGC AACAAAGCTA ATCCTTAACT ATCCAAAAGC CAAAATGGAA
GATGGCCAGG CTGCTGAAAG CCTTCACAAG ACCATTGTTA AGAGGCGAAT GTCCCATGTG
AGTGGAGGAG GATCCATAGA TTTGTCTGAC ACAGACTCCC TACAGGAATG GATCAACATG
ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG
GCAACCTATA GCCCACCCAT GGGTCCAGTC AGTGAACGTA AGGGTTCTAT GATTTCAGTG
ATGTCTTCAG AGGGAAACGC AGATACACCT GTCAGCAAAT TTATGGATCG GCTGTTGTCC
TTAATGGTGT GTAACCATGA GAAAGTGGGA CTTCAAATAC GGACCAATGT TAAGGATCTG
GTGGGTCTAG AATTGAGTCC TGCTCTGTAT CCAATGCTAT TTAACAAATT GAAGAATACC
ATCAGCAAGT TTTTTGACTC CCAAGGACAG GTTTTATTGA CTGATACCAA TACTCAATTT
GTAGAACAAA CCATAGCTAT AATGAAGAAC TTGCTAGATA ATCATACTGA AGGCAGCTCT
GAACATCTAG GGCAAGCTAG CATTGAAACA ATGATGTTAA ATCTGGTCAG GTATGTTCGT
GTGCTTGGGA ATATGGTCCA TGCAATTCAA ATAAAAACGA AACTGTGTCA ATTAGTTGAA
GTAATGATGG CAAGGAGAGA TGACCTCTCA TTTTGCCAAG AGATGAAATT TAGGAATAAG
ATGGTAGAAT ACCTGACAGA CTGGGTTATG GGAACATCAA ACCAAGCAGC AGATGATGAT
GTAAAATGTC TTACAAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTAGT TTCACTTCTA
GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA GATGGTGTGG AATTGATGGA AGCCAAATCA
CAGTTATTTC TTAAATACTT CACATTATTT ATGAACCTTT TGAATGACTG CAGTGAAGTT
GAAGATGAAA GTGCGCAAAC AGGTGGCAGG AAACGTGGCA TGTCTCGGAG GCTGGCATCA
CTGAGGCACT GTACGGTCCT TGCAATGTCA AACTTACTCA ATGCCAACGT AGACAGTGGT
CTCATGCACT CCATAGGCTT AGGTTACCAC AAGGATCTCC AGACAAGAGC TACATTTATG
GAAGTTCTGA CAAAAATCCT TCAACAAGGC ACAGAATTTG ACACACTTGC AGAAACAGTA
TTGGCTGATC GGTTTGAGAG ATTGGTGGAA CTGGTCACAA TGATGGGTGA TCAAGGAGAA
CTCCCTATAG CGATGGCTCT GGCCAATGTG GTTCCTTGTT CTCAGTGGGA TGAACTAGCT
CGAGTTCTGG TTACTCTGTT TGATTCTCGG CATTTACTCT ACCAACTGCT CTGGAACATG
TTTTCTAAAG AAGTAGAATT GGCAGACTCC ATGCAGACTC TCTTCCGAGG CAACAGCTTG
GCCAGTAAAA TAATGACATT CTGTTTCAAG GTATATGGTG CTACCTATCT ACAAAAACTC
CTGGATCCTT TATTACGAAT TGTGATCACA TCCTCTGATT GGCAACATGT TAGCTTTGAA
GTGGATCCTA CCAGGTTAGA ACCATCAGAG AGCCTTGAGG AAAACCAGCG GAACCTCCTT
CAGATGACTG AAAAGTTCTT CCATGCCATC ATCAGTTCCT CCTCAGAATT CCCCCCTCAA
CTTCGAAGTG TGTGCCACTG TTTATACCAG GCAACTTGCC ACTCCCTACT GAATAAAGCT
ACAGTAAAAG AAAAAAAGGA AAACAAAAAA TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC
AGCATCGGTG CAGTAGGAAG TGCCATGTTC CTCAGATTTA TCAATCCTGC CATTGTCTCA
CCGTATGAAG CAGGGATTTT AGATAAAAAG CCACCACCTA GAATCGAAAG GGGCTTGAAG
TTAATGTCAA AGATACTTCA GAGTATTGCC AATCATGTTC TCTTCACAAA AGAAGAACAT
ATGCGGCCTT TCAATGATTT TGTGAAAAGC AACTTTGATG CAGCACGCAG GTTTTTCCTT
GATATAGCAT CTGATTGTCC TACAAGTGAT GCAGTAAATC ATAGTCTTTC CTTCATAAGT
GACGGCAATG TGCTTGCTTT ACATCGTCTA CTCTGGAACA ATCAGGAGAA AATTGGGCAG
TATCTTTCCA GCAACAGGGA TCATAAAGCT GTTGGAAGAC GACCTTTTGA TAAGATGGCA
ACACTTCTTG CATACCTGGG TCCTCCAGAG CACAAACCTG TGGCAGATAC ACACTGGTCC
AGCCTTAACC TTACCAGTTC AAAGTTTGAG GAATTTATGA CTAGGCATCA GGTACATGAA
AAAGAAGAAT TCAAGGCTTT GAAAACGTTA AGTATTTTCT ACCAAGCTGG GACTTCCAAA
GCTGGGAATC CTATTTTTTA TTATGTTGCA CGGAGGTTCA AAACTGGTCA AATCAATGGT
GATTTGCTGA TATACCATGT CTTACTGACT TTAAAGCCAT ATTATGCAAA GCCATATGAA
ATTGTAGTGG ACCTTACCCA TACCGGGCCT AGCAATCGCT TTAAAACAGA CTTTCTCTCT
AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT TACGACAACG TCTCCGCAGT CTATATCTAT
AACTGTAACT CCTGGGTCAG GGAGTACACC AAGTATCATG AGCGGCTGCT GACTGGCCTC
AAAGGTAGCA AAAGGCTTGT TTTCATAGAC TGTCCTGGGA AACTGGCTGA GCACATAGAG
CATGAACAAC AGAAACTACC TGCTGCCACC TTGGCTTTAG AAGAGGACCT GAAGGTATTC
CACAATGCTC TCAAGCTAGC TCACAAAGAC ACCAAAGTTT CTATTAAAGT TGGTTCTACT
GCTGTCCAAG TAACTTCAGC AGAGCGAACA AAAGTCCTAG GGCAATCAGT CTTTCTAAAT
GACATTTATT ATGCTTCGGA AATTGAAGAA ATCTGCCTAG TAGATGAGAA CCAGTTCACC
TTAACCATTG CAAACCAGGG CACGCCGCTC ACCTTCATGC ACCAGGAGTG TGAAGCCATT
GTCCAGTCTA TCATTCATAT CCGGACCCGC TGGGAACTGT CACAGCCCGA CTCTATCCCC
CAACACACCA AGATTCGGCC AAAAGATGTC CCTGGGACAC TGCTCAATAT CGCATTACTT
AATTTAGGCA GTTCTGACCC GAGTTTACGG TCAGCTGCCT ATAATCTTCT GTGTGCCTTA
ACTTGTACCT TTAATTTAAA AATCGAGGGC CAGTTACTAG AGACATCAGG TTTATGTATC
CCTGCCAACA ACACCCTCTT TATTGTCTCT ATTAGTAAGA CACTGGCAGC CAATGAGCCA
CACCTCACGT TAGAATTTTT GGAAGAGTGT ATTTCTGGAT TTAGCAAATC TAGTATTGAA
TTGAAACACC TTTGTTTGGA ATACATGACT CCATGGCTGT CAAATCTAGT TCGTTTTTGC
AAGCATAATG ATGATGCCAA ACGACAAAGA GTTACTGCTA TTCTTGACAA GCTGATAACA
ATGACCATCA ATGAAAAACA GATGTACCCA TCTATTCAAG CAAAAATATG GGGAAGCCTT
GGGCAGATTA CAGATCTGCT TGATGTTGTA CTAGACAGTT TCATCAAAAC CAGTGCAACA
GGTGGCTTGG GATCAATAAA AGCTGAGGTG ATGGCAGATA CTGCTGTAGC TTTGGCTTCT
GGAAATGTGA AATTGGTTTC AAGCAAGGTT ATTGGAAGGA TGTGCAAAAT AATTGACAAG
ACATGCTTAT CTCCAACTCC TACTTTAGAA CAACATCTTA TGTGGGATGA TATTGCTATT
TTAGCACGCT ACATGCTGAT GCTGTCCTTC AACAATTCCC TTGATGTGGC AGCTCATCTT
CCCTACCTCT TCCACGTTGT TACTTTCTTA GTAGCCACAG GTCCGCTCTC CCTTAGAGCT
TCCACACATG GACTGGTCAT TAATATCATT CACTCTCTGT GTACTTGTTC ACAGCTTCAT
TTTAGTGAAG AGACCAAGCA AGTTTTGAGA CTCAGTCTGA CAGAGTTCTC ATTACCCAAA
TTTTACTTGC TGTTTGGCAT TAGCAAAGTC AAGTCAGCTG CTGTCATTGC CTTCCGTTCC
AGTTACCGGG ACAGGTCATT CTCTCCTGGC TCCTATGAGA GAGAGACTTT TGCTTTGACA
TCCTTGGAAA CAGTCACAGA AGCTTTGTTG GAGATCATGG AGGCATGCAT GAGAGATATT
CCAACGTGCA AGTGGCTGGA CCAGTGGACA GAACTAGCTC AAAGATTTGC ATTCCAATAT
AATCCATCCC TGCAACCAAG AGCTCTTGTT GTCTTTGGGT GTATTAGCAA ACGAGTGTCT
CATGGGCAGA TAAAGCAGAT AATCCGTATT CTTAGCAAGG CACTTGAGAG TTGCTTAAAA
GGACCTGACA CTTACAACAG TCAAGTTCTG ATAGAAGCTA CAGTAATAGC ACTAACCAAA
TTACAGCCAC TTCTTAATAA GGACTCGCCT CTGCACAAAG CCCTCTTTTG GGTAGCTGTG
GCTGTGCTGC AGCTTGATGA GGTCAACTTG TATTCAGCAG GTACCGCACT TCTTGAACAA
AACCTGCATA CTTTAGATAG TCTCCGTATA TTCAATGACA AGAGTCCAGA GGAAGTATTT
ATGGCAATCC GGAATCCTCT GGAGTGGCAC TGCAAGCAAA TGGATCATTT TGTTGGACTC
AATTTCAACT CTAACTTTAA CTTTGCATTG GTTGGACACC TTTTAAAAGG GTACAGGCAT
CCTTCACCTG CTATTGTTGC AAGAACAGTC AGAATTTTAC ATACACTACT AACTCTGGTT
AACAAACACA GAAATTGTGA CAAATTTGAA GTGAATACAC AGAGCGTGGC CTACTTAGCA
GCTTTACTTA CAGTGTCTGA AGAAGTTCGA AGTCGCTGCA GCCTAAAACA TAGAAAGTCA
CTTCTTCTTA CTGATATTTC AATGGAAAAT GTTCCTATGG ATACATATCC CATTCATCAT
GGTGACCCTT CCTATAGGAC ACTAAAGGAG ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT
GAAGGATACC TTGCAGCCAC CTATCCAACT GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA
TCCATGAGCC TGGACATGGG GCAACCTTCT CAGGCCAACA CTAAGAAGTT GCTTGGAACA
AGGAAAAGTT TTGATCACTT GATATCAGAC ACAAAGGCTC CTAAAAGGCA AGAAATGGAA
TCAGGGATCA CAACACCCCC CAAAATGAGG AGAGTAGCAG AAACTGATTA TGAAATGGAA
ACTCAGAGGA TTTCCTCATC ACAACAGCAC CCACATTTAC GTAAAGTTTC AGTGTCTGAA
TCAAATGTTC TCTTGGATGA AGAAGTACTT ACTGATCCGA AGATCCAGGC GCTGCTTCTT
ACTGTTCTAG CTACACTGGT AAAATATACC ACAGATGAGT TTGATCAACG AATTCTTTAT
GAATACTTAG CAGAGGCCAG TGTTGTGTTT CCCAAAGTCT TTCCTGTTGT GCATAATTTG
TTGGACTCTA AGATCAACAC CCTGTTATCA TTGTGCCAAG ATCCAAATTT GTTAAATCCA
ATCCATGGAA TTGTGCAGAG TGTGGTGTAC CATGAAGAAT CCCCACCACA ATACCAAACA
TCTTACCTGC AAAGTTTTGG TTTTAATGGC TTGTGGCGGT TTGCAGGACC GTTTTCAAAG
CAAACACAAA TTCCAGACTA TGCTGAGCTT ATTGTTAAGT TTCTTGATGC CTTGATTGAC
ACGTACCTGC CTGGAATTGA TGAAGAAACC AGTGAAGAAT CCCTCCTGAC TCCCACATCT
CCTTACCCTC CTGCACTGCA GAGCCAGCTT AGTATCACTG CCAACCTTAA CCTTTCTAAT
TCCATGACCT CACTTGCAAC TTCCCAGCAT TCCCCAGCTT CTCTGCCTTG CTCTAAATCA
GCAGTTTTCA TGCAGCTGTT CCCTCATCAA GGAATCGACA AGGAGAACGT TGAACTCTCC
CCTACCACTG GCCACTGTAA CAGTGGACGA ACTCGCCACG GATCCGCAAG CCAAGTGCAG
AAGCAAAGAA GCGCTGGCAG TTTCAAACGT AATAGCATTA AGAAGATCGT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50429
Clone ID Related Accession (Same CDS sequence) XM_006721923 , XM_011524853 , XM_011524854 , XM_011524855 , XM_011524856
Accession Version XM_011524853.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8565bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neurofibromin isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
ATGTGGAATG CAAAGGAGAG GCTTCCAATA AAAACAGGAC AGCAGAACAC ACATACCAAA 
GTCAGTACTG AGCACAACAA GGAATGTCTA ATCAATATTT CCAAATACAA GTTTTCTTTG
GTTATAAGCG GCCTCACTAC TATTTTAAAG AATGTTAACA ATATGAGAAT ATTTGGAGAA
GCTGCTGAAA AAAATTTATA TCTCTCTCAG TTGATTATAT TGGATACACT GGAAAAATGT
CTTGCTGGGC AACCAAAGGA CACAATGAGA TTAGATGAAA CGATGCTGGT CAAACAGTTG
CTGCCAGAAA TCTGCCATTT TCTTCACACC TGTCGTGAAG GAAACCAGCA TGCAGCTGAA
CTTCGGAATT CTGCCTCTGG GGTTTTATTT TCTCTCAGCT GCAACAACTT CAATGCAGTC
TTTAGTCGCA TTTCTACCAG GTTACAGGAA TTAACTGTTT GTTCAGAAGA CAATGTTGAT
GTTCATGATA TAGAATTGTT ACAGTATATC AATGTGGATT GTGCAAAATT AAAACGACTC
CTGAAGGAAA CAGCATTTAA ATTTAAAGCC CTAAAGAAGG TTGCGCAGTT AGCAGTTATA
AATAGCCTGG AAAAGGCATT TTGGAACTGG GTAGAAAATT ATCCAGATGA ATTTACAAAA
CTGTACCAGA TCCCACAGAC TGATATGGCT GAATGTGCAG AAAAGCTATT TGACTTGGTG
GATGGTTTTG CTGAAAGCAC CAAACGTAAA GCAGCAGTTT GGCCACTACA AATCATTCTC
CTTATCTTGT GTCCAGAAAT AATCCAGGAT ATATCCAAAG ACGTGGTTGA TGAAAACAAC
ATGAATAAGA AGTTATTTCT GGACAGTCTA CGAAAAGCTC TTGCTGGCCA TGGAGGAAGT
AGGCAGCTGA CAGAAAGTGC TGCAATTGCC TGTGTCAAAC TGTGTAAAGC AAGTACTTAC
ATCAATTGGG AAGATAACTC TGTCATTTTC CTACTTGTTC AGTCCATGGT GGTTGATCTT
AAGAACCTGC TTTTTAATCC AAGTAAGCCA TTCTCAAGAG GCAGTCAGCC TGCAGATGTG
GATCTAATGA TTGACTGCCT TGTTTCTTGC TTTCGTATAA GCCCTCACAA CAACCAACAC
TTTAAGATCT GCCTGGCTCA GAATTCACCT TCTACATTTC ACTATGTGCT GGTAAATTCA
CTCCATCGAA TCATCACCAA TAGCACTTTC AAGCATGGAC TTGGCACTGC TTCCGCATTG
GATTGGTGGC CTAAGATTGA TGCTGTGTAT TGTCACTCGG TTGAACTTCG AAATATGTTT
GGTGAAACAC TTCATAAAGC AGTGCAAGGT TGTGGAGCAC ACCCAGCAAT ACGAATGGCA
CCGAGTCTTA CATTTAAAGA AAAAGTAACA AGCCTTAAAT TTAAAGAAAA ACCTACAGAC
CTGGAGACAA GAAGCTATAA GTATCTTCTC TTGTCCATGG TGAAACTAAT TCATGCAGAT
CCAAAGCTCT TGCTTTGTAA TCCAAGAAAA CAGGGGCCCG AAACCCAAGG CAGTACAGCA
GAATTAATTA CAGGGCTCGT CCAACTGGTC CCTCAGTCAC ACATGCCAGA GATTGCTCAG
GAAGCAATGG AGGCTCTGCT GGTTCTTCAT CAGTTAGATA GCATTGATTT GTGGAATCCT
GATGCTCCTG TAGAAACATT TTGGGAGATT AGCTCACAAA TGCTTTTTTA CATCTGCAAG
AAATTAACTA GTCATCAAAT GCTTAGTAGC ACAGAAATTC TCAAGTGGTT GCGGGAAATA
TTGATCTGCA GGAATAAATT TCTTCTTAAA AATAAGCAGG CAGATAGAAG TTCCTGTCAC
TTTCTCCTTT TTTACGGGGT AGGATGTGAT ATTCCTTCTA GTGGAAATAC CAGTCAAATG
TCCATGGATC ATGAAGAATT ACTACGTACT CCTGGAGCCT CTCTCCGGAA GGGAAAAGGG
AACTCCTCTA TGGATAGTGC AGCAGGATGC AGCGGAACCC CCCCGATTTG CCGACAAGCC
CAGACCAAAC TAGAAGTGGC CCTGTACATG TTTCTGTGGA ACCCTGACAC TGAAGCTGTT
CTGGTTGCCA TGTCCTGTTT CCGCCACCTC TGTGAGGAAG CAGATATCCG GTGTGGGGTG
GATGAAGTGT CAGTGCATAA CCTCTTGCCC AACTATAACA CATTCATGGA GTTTGCCTCT
GTCAGCAATA TGATGTCAAC AGGAAGAGCA GCACTTCAGA AAAGAGTGAT GGCACTGCTG
AGGCGCATTG AGCATCCCAC TGCAGGAAAC ACTGAGGCTT GGGAAGATAC ACATGCAAAA
TGGGAACAAG CAACAAAGCT AATCCTTAAC TATCCAAAAG CCAAAATGGA AGATGGCCAG
GCTGCTGAAA GCCTTCACAA GACCATTGTT AAGAGGCGAA TGTCCCATGT GAGTGGAGGA
GGATCCATAG ATTTGTCTGA CACAGACTCC CTACAGGAAT GGATCAACAT GACTGGCTTC
CTTTGTGCCC TTGGGGGAGT GTGCCTCCAG CAGAGAAGCA ATTCTGGCCT GGCAACCTAT
AGCCCACCCA TGGGTCCAGT CAGTGAACGT AAGGGTTCTA TGATTTCAGT GATGTCTTCA
GAGGGAAACG CAGATACACC TGTCAGCAAA TTTATGGATC GGCTGTTGTC CTTAATGGTG
TGTAACCATG AGAAAGTGGG ACTTCAAATA CGGACCAATG TTAAGGATCT GGTGGGTCTA
GAATTGAGTC CTGCTCTGTA TCCAATGCTA TTTAACAAAT TGAAGAATAC CATCAGCAAG
TTTTTTGACT CCCAAGGACA GGTTTTATTG ACTGATACCA ATACTCAATT TGTAGAACAA
ACCATAGCTA TAATGAAGAA CTTGCTAGAT AATCATACTG AAGGCAGCTC TGAACATCTA
GGGCAAGCTA GCATTGAAAC AATGATGTTA AATCTGGTCA GGTATGTTCG TGTGCTTGGG
AATATGGTCC ATGCAATTCA AATAAAAACG AAACTGTGTC AATTAGTTGA AGTAATGATG
GCAAGGAGAG ATGACCTCTC ATTTTGCCAA GAGATGAAAT TTAGGAATAA GATGGTAGAA
TACCTGACAG ACTGGGTTAT GGGAACATCA AACCAAGCAG CAGATGATGA TGTAAAATGT
CTTACAAGAG ATTTGGACCA GGCAAGCATG GAAGCAGTAG TTTCACTTCT AGCTGGTCTC
CCTCTGCAGC CTGAAGAAGG AGATGGTGTG GAATTGATGG AAGCCAAATC ACAGTTATTT
CTTAAATACT TCACATTATT TATGAACCTT TTGAATGACT GCAGTGAAGT TGAAGATGAA
AGTGCGCAAA CAGGTGGCAG GAAACGTGGC ATGTCTCGGA GGCTGGCATC ACTGAGGCAC
TGTACGGTCC TTGCAATGTC AAACTTACTC AATGCCAACG TAGACAGTGG TCTCATGCAC
TCCATAGGCT TAGGTTACCA CAAGGATCTC CAGACAAGAG CTACATTTAT GGAAGTTCTG
ACAAAAATCC TTCAACAAGG CACAGAATTT GACACACTTG CAGAAACAGT ATTGGCTGAT
CGGTTTGAGA GATTGGTGGA ACTGGTCACA ATGATGGGTG ATCAAGGAGA ACTCCCTATA
GCGATGGCTC TGGCCAATGT GGTTCCTTGT TCTCAGTGGG ATGAACTAGC TCGAGTTCTG
GTTACTCTGT TTGATTCTCG GCATTTACTC TACCAACTGC TCTGGAACAT GTTTTCTAAA
GAAGTAGAAT TGGCAGACTC CATGCAGACT CTCTTCCGAG GCAACAGCTT GGCCAGTAAA
ATAATGACAT TCTGTTTCAA GGTATATGGT GCTACCTATC TACAAAAACT CCTGGATCCT
TTATTACGAA TTGTGATCAC ATCCTCTGAT TGGCAACATG TTAGCTTTGA AGTGGATCCT
ACCAGGTTAG AACCATCAGA GAGCCTTGAG GAAAACCAGC GGAACCTCCT TCAGATGACT
GAAAAGTTCT TCCATGCCAT CATCAGTTCC TCCTCAGAAT TCCCCCCTCA ACTTCGAAGT
GTGTGCCACT GTTTATACCA GGCAACTTGC CACTCCCTAC TGAATAAAGC TACAGTAAAA
GAAAAAAAGG AAAACAAAAA ATCAGTGGTT AGCCAGCGTT TCCCTCAGAA CAGCATCGGT
GCAGTAGGAA GTGCCATGTT CCTCAGATTT ATCAATCCTG CCATTGTCTC ACCGTATGAA
GCAGGGATTT TAGATAAAAA GCCACCACCT AGAATCGAAA GGGGCTTGAA GTTAATGTCA
AAGATACTTC AGAGTATTGC CAATCATGTT CTCTTCACAA AAGAAGAACA TATGCGGCCT
TTCAATGATT TTGTGAAAAG CAACTTTGAT GCAGCACGCA GGTTTTTCCT TGATATAGCA
TCTGATTGTC CTACAAGTGA TGCAGTAAAT CATAGTCTTT CCTTCATAAG TGACGGCAAT
GTGCTTGCTT TACATCGTCT ACTCTGGAAC AATCAGGAGA AAATTGGGCA GTATCTTTCC
AGCAACAGGG ATCATAAAGC TGTTGGAAGA CGACCTTTTG ATAAGATGGC AACACTTCTT
GCATACCTGG GTCCTCCAGA GCACAAACCT GTGGCAGATA CACACTGGTC CAGCCTTAAC
CTTACCAGTT CAAAGTTTGA GGAATTTATG ACTAGGCATC AGGTACATGA AAAAGAAGAA
TTCAAGGCTT TGAAAACGTT AAGTATTTTC TACCAAGCTG GGACTTCCAA AGCTGGGAAT
CCTATTTTTT ATTATGTTGC ACGGAGGTTC AAAACTGGTC AAATCAATGG TGATTTGCTG
ATATACCATG TCTTACTGAC TTTAAAGCCA TATTATGCAA AGCCATATGA AATTGTAGTG
GACCTTACCC ATACCGGGCC TAGCAATCGC TTTAAAACAG ACTTTCTCTC TAAGTGGTTT
GTTGTTTTTC CTGGCTTTGC TTACGACAAC GTCTCCGCAG TCTATATCTA TAACTGTAAC
TCCTGGGTCA GGGAGTACAC CAAGTATCAT GAGCGGCTGC TGACTGGCCT CAAAGGTAGC
AAAAGGCTTG TTTTCATAGA CTGTCCTGGG AAACTGGCTG AGCACATAGA GCATGAACAA
CAGAAACTAC CTGCTGCCAC CTTGGCTTTA GAAGAGGACC TGAAGGTATT CCACAATGCT
CTCAAGCTAG CTCACAAAGA CACCAAAGTT TCTATTAAAG TTGGTTCTAC TGCTGTCCAA
GTAACTTCAG CAGAGCGAAC AAAAGTCCTA GGGCAATCAG TCTTTCTAAA TGACATTTAT
TATGCTTCGG AAATTGAAGA AATCTGCCTA GTAGATGAGA ACCAGTTCAC CTTAACCATT
GCAAACCAGG GCACGCCGCT CACCTTCATG CACCAGGAGT GTGAAGCCAT TGTCCAGTCT
ATCATTCATA TCCGGACCCG CTGGGAACTG TCACAGCCCG ACTCTATCCC CCAACACACC
AAGATTCGGC CAAAAGATGT CCCTGGGACA CTGCTCAATA TCGCATTACT TAATTTAGGC
AGTTCTGACC CGAGTTTACG GTCAGCTGCC TATAATCTTC TGTGTGCCTT AACTTGTACC
TTTAATTTAA AAATCGAGGG CCAGTTACTA GAGACATCAG GTTTATGTAT CCCTGCCAAC
AACACCCTCT TTATTGTCTC TATTAGTAAG ACACTGGCAG CCAATGAGCC ACACCTCACG
TTAGAATTTT TGGAAGAGTG TATTTCTGGA TTTAGCAAAT CTAGTATTGA ATTGAAACAC
CTTTGTTTGG AATACATGAC TCCATGGCTG TCAAATCTAG TTCGTTTTTG CAAGCATAAT
GATGATGCCA AACGACAAAG AGTTACTGCT ATTCTTGACA AGCTGATAAC AATGACCATC
AATGAAAAAC AGATGTACCC ATCTATTCAA GCAAAAATAT GGGGAAGCCT TGGGCAGATT
ACAGATCTGC TTGATGTTGT ACTAGACAGT TTCATCAAAA CCAGTGCAAC AGGTGGCTTG
GGATCAATAA AAGCTGAGGT GATGGCAGAT ACTGCTGTAG CTTTGGCTTC TGGAAATGTG
AAATTGGTTT CAAGCAAGGT TATTGGAAGG ATGTGCAAAA TAATTGACAA GACATGCTTA
TCTCCAACTC CTACTTTAGA ACAACATCTT ATGTGGGATG ATATTGCTAT TTTAGCACGC
TACATGCTGA TGCTGTCCTT CAACAATTCC CTTGATGTGG CAGCTCATCT TCCCTACCTC
TTCCACGTTG TTACTTTCTT AGTAGCCACA GGTCCGCTCT CCCTTAGAGC TTCCACACAT
GGACTGGTCA TTAATATCAT TCACTCTCTG TGTACTTGTT CACAGCTTCA TTTTAGTGAA
GAGACCAAGC AAGTTTTGAG ACTCAGTCTG ACAGAGTTCT CATTACCCAA ATTTTACTTG
CTGTTTGGCA TTAGCAAAGT CAAGTCAGCT GCTGTCATTG CCTTCCGTTC CAGTTACCGG
GACAGGTCAT TCTCTCCTGG CTCCTATGAG AGAGAGACTT TTGCTTTGAC ATCCTTGGAA
ACAGTCACAG AAGCTTTGTT GGAGATCATG GAGGCATGCA TGAGAGATAT TCCAACGTGC
AAGTGGCTGG ACCAGTGGAC AGAACTAGCT CAAAGATTTG CATTCCAATA TAATCCATCC
CTGCAACCAA GAGCTCTTGT TGTCTTTGGG TGTATTAGCA AACGAGTGTC TCATGGGCAG
ATAAAGCAGA TAATCCGTAT TCTTAGCAAG GCACTTGAGA GTTGCTTAAA AGGACCTGAC
ACTTACAACA GTCAAGTTCT GATAGAAGCT ACAGTAATAG CACTAACCAA ATTACAGCCA
CTTCTTAATA AGGACTCGCC TCTGCACAAA GCCCTCTTTT GGGTAGCTGT GGCTGTGCTG
CAGCTTGATG AGGTCAACTT GTATTCAGCA GGTACCGCAC TTCTTGAACA AAACCTGCAT
ACTTTAGATA GTCTCCGTAT ATTCAATGAC AAGAGTCCAG AGGAAGTATT TATGGCAATC
CGGAATCCTC TGGAGTGGCA CTGCAAGCAA ATGGATCATT TTGTTGGACT CAATTTCAAC
TCTAACTTTA ACTTTGCATT GGTTGGACAC CTTTTAAAAG GGTACAGGCA TCCTTCACCT
GCTATTGTTG CAAGAACAGT CAGAATTTTA CATACACTAC TAACTCTGGT TAACAAACAC
AGAAATTGTG ACAAATTTGA AGTGAATACA CAGAGCGTGG CCTACTTAGC AGCTTTACTT
ACAGTGTCTG AAGAAGTTCG AAGTCGCTGC AGCCTAAAAC ATAGAAAGTC ACTTCTTCTT
ACTGATATTT CAATGGAAAA TGTTCCTATG GATACATATC CCATTCATCA TGGTGACCCT
TCCTATAGGA CACTAAAGGA GACTCAGCCA TGGTCCTCTC CCAAAGGTTC TGAAGGATAC
CTTGCAGCCA CCTATCCAAC TGTCGGCCAG ACCAGTCCCC GAGCCAGGAA ATCCATGAGC
CTGGACATGG GGCAACCTTC TCAGGCCAAC ACTAAGAAGT TGCTTGGAAC AAGGAAAAGT
TTTGATCACT TGATATCAGA CACAAAGGCT CCTAAAAGGC AAGAAATGGA ATCAGGGATC
ACAACACCCC CCAAAATGAG GAGAGTAGCA GAAACTGATT ATGAAATGGA AACTCAGAGG
ATTTCCTCAT CACAACAGCA CCCACATTTA CGTAAAGTTT CAGTGTCTGA ATCAAATGTT
CTCTTGGATG AAGAAGTACT TACTGATCCG AAGATCCAGG CGCTGCTTCT TACTGTTCTA
GCTACACTGG TAAAATATAC CACAGATGAG TTTGATCAAC GAATTCTTTA TGAATACTTA
GCAGAGGCCA GTGTTGTGTT TCCCAAAGTC TTTCCTGTTG TGCATAATTT GTTGGACTCT
AAGATCAACA CCCTGTTATC ATTGTGCCAA GATCCAAATT TGTTAAATCC AATCCATGGA
ATTGTGCAGA GTGTGGTGTA CCATGAAGAA TCCCCACCAC AATACCAAAC ATCTTACCTG
CAAAGTTTTG GTTTTAATGG CTTGTGGCGG TTTGCAGGAC CGTTTTCAAA GCAAACACAA
ATTCCAGACT ATGCTGAGCT TATTGTTAAG TTTCTTGATG CCTTGATTGA CACGTACCTG
CCTGGAATTG ATGAAGAAAC CAGTGAAGAA TCCCTCCTGA CTCCCACATC TCCTTACCCT
CCTGCACTGC AGAGCCAGCT TAGTATCACT GCCAACCTTA ACCTTTCTAA TTCCATGACC
TCACTTGCAA CTTCCCAGCA TTCCCCAGCT TCTCTGCCTT GCTCTAAATC AGCAGTTTTC
ATGCAGCTGT TCCCTCATCA AGGAATCGAC AAGGAGAACG TTGAACTCTC CCCTACCACT
GGCCACTGTA ACAGTGGACG AACTCGCCAC GGATCCGCAA GCCAAGTGCA GAAGCAAAGA
AGCGCTGGCA GTTTCAAACG TAATAGCATT AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523155.1
CDS1831..10395
Misc Feature(1)5380..6492(+)
Misc Feature(2)5413..6474(+)
Misc Feature(3)5527..6195(+)
Misc Feature(4)6562..7005(+)
Misc Feature(5)6631..6936(+)
Misc Feature(6)6835..6933(+)
Misc Feature(7)7003..7332(+)
Misc Feature(8)7003..7332(+)
Translation

Target ORF information:

RefSeq Version XM_011524853
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524853

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
ATGTGGAATG CAAAGGAGAG GCTTCCAATA AAAACAGGAC AGCAGAACAC ACATACCAAA 
GTCAGTACTG AGCACAACAA GGAATGTCTA ATCAATATTT CCAAATACAA GTTTTCTTTG
GTTATAAGCG GCCTCACTAC TATTTTAAAG AATGTTAACA ATATGAGAAT ATTTGGAGAA
GCTGCTGAAA AAAATTTATA TCTCTCTCAG TTGATTATAT TGGATACACT GGAAAAATGT
CTTGCTGGGC AACCAAAGGA CACAATGAGA TTAGATGAAA CGATGCTGGT CAAACAGTTG
CTGCCAGAAA TCTGCCATTT TCTTCACACC TGTCGTGAAG GAAACCAGCA TGCAGCTGAA
CTTCGGAATT CTGCCTCTGG GGTTTTATTT TCTCTCAGCT GCAACAACTT CAATGCAGTC
TTTAGTCGCA TTTCTACCAG GTTACAGGAA TTAACTGTTT GTTCAGAAGA CAATGTTGAT
GTTCATGATA TAGAATTGTT ACAGTATATC AATGTGGATT GTGCAAAATT AAAACGACTC
CTGAAGGAAA CAGCATTTAA ATTTAAAGCC CTAAAGAAGG TTGCGCAGTT AGCAGTTATA
AATAGCCTGG AAAAGGCATT TTGGAACTGG GTAGAAAATT ATCCAGATGA ATTTACAAAA
CTGTACCAGA TCCCACAGAC TGATATGGCT GAATGTGCAG AAAAGCTATT TGACTTGGTG
GATGGTTTTG CTGAAAGCAC CAAACGTAAA GCAGCAGTTT GGCCACTACA AATCATTCTC
CTTATCTTGT GTCCAGAAAT AATCCAGGAT ATATCCAAAG ACGTGGTTGA TGAAAACAAC
ATGAATAAGA AGTTATTTCT GGACAGTCTA CGAAAAGCTC TTGCTGGCCA TGGAGGAAGT
AGGCAGCTGA CAGAAAGTGC TGCAATTGCC TGTGTCAAAC TGTGTAAAGC AAGTACTTAC
ATCAATTGGG AAGATAACTC TGTCATTTTC CTACTTGTTC AGTCCATGGT GGTTGATCTT
AAGAACCTGC TTTTTAATCC AAGTAAGCCA TTCTCAAGAG GCAGTCAGCC TGCAGATGTG
GATCTAATGA TTGACTGCCT TGTTTCTTGC TTTCGTATAA GCCCTCACAA CAACCAACAC
TTTAAGATCT GCCTGGCTCA GAATTCACCT TCTACATTTC ACTATGTGCT GGTAAATTCA
CTCCATCGAA TCATCACCAA TAGCACTTTC AAGCATGGAC TTGGCACTGC TTCCGCATTG
GATTGGTGGC CTAAGATTGA TGCTGTGTAT TGTCACTCGG TTGAACTTCG AAATATGTTT
GGTGAAACAC TTCATAAAGC AGTGCAAGGT TGTGGAGCAC ACCCAGCAAT ACGAATGGCA
CCGAGTCTTA CATTTAAAGA AAAAGTAACA AGCCTTAAAT TTAAAGAAAA ACCTACAGAC
CTGGAGACAA GAAGCTATAA GTATCTTCTC TTGTCCATGG TGAAACTAAT TCATGCAGAT
CCAAAGCTCT TGCTTTGTAA TCCAAGAAAA CAGGGGCCCG AAACCCAAGG CAGTACAGCA
GAATTAATTA CAGGGCTCGT CCAACTGGTC CCTCAGTCAC ACATGCCAGA GATTGCTCAG
GAAGCAATGG AGGCTCTGCT GGTTCTTCAT CAGTTAGATA GCATTGATTT GTGGAATCCT
GATGCTCCTG TAGAAACATT TTGGGAGATT AGCTCACAAA TGCTTTTTTA CATCTGCAAG
AAATTAACTA GTCATCAAAT GCTTAGTAGC ACAGAAATTC TCAAGTGGTT GCGGGAAATA
TTGATCTGCA GGAATAAATT TCTTCTTAAA AATAAGCAGG CAGATAGAAG TTCCTGTCAC
TTTCTCCTTT TTTACGGGGT AGGATGTGAT ATTCCTTCTA GTGGAAATAC CAGTCAAATG
TCCATGGATC ATGAAGAATT ACTACGTACT CCTGGAGCCT CTCTCCGGAA GGGAAAAGGG
AACTCCTCTA TGGATAGTGC AGCAGGATGC AGCGGAACCC CCCCGATTTG CCGACAAGCC
CAGACCAAAC TAGAAGTGGC CCTGTACATG TTTCTGTGGA ACCCTGACAC TGAAGCTGTT
CTGGTTGCCA TGTCCTGTTT CCGCCACCTC TGTGAGGAAG CAGATATCCG GTGTGGGGTG
GATGAAGTGT CAGTGCATAA CCTCTTGCCC AACTATAACA CATTCATGGA GTTTGCCTCT
GTCAGCAATA TGATGTCAAC AGGAAGAGCA GCACTTCAGA AAAGAGTGAT GGCACTGCTG
AGGCGCATTG AGCATCCCAC TGCAGGAAAC ACTGAGGCTT GGGAAGATAC ACATGCAAAA
TGGGAACAAG CAACAAAGCT AATCCTTAAC TATCCAAAAG CCAAAATGGA AGATGGCCAG
GCTGCTGAAA GCCTTCACAA GACCATTGTT AAGAGGCGAA TGTCCCATGT GAGTGGAGGA
GGATCCATAG ATTTGTCTGA CACAGACTCC CTACAGGAAT GGATCAACAT GACTGGCTTC
CTTTGTGCCC TTGGGGGAGT GTGCCTCCAG CAGAGAAGCA ATTCTGGCCT GGCAACCTAT
AGCCCACCCA TGGGTCCAGT CAGTGAACGT AAGGGTTCTA TGATTTCAGT GATGTCTTCA
GAGGGAAACG CAGATACACC TGTCAGCAAA TTTATGGATC GGCTGTTGTC CTTAATGGTG
TGTAACCATG AGAAAGTGGG ACTTCAAATA CGGACCAATG TTAAGGATCT GGTGGGTCTA
GAATTGAGTC CTGCTCTGTA TCCAATGCTA TTTAACAAAT TGAAGAATAC CATCAGCAAG
TTTTTTGACT CCCAAGGACA GGTTTTATTG ACTGATACCA ATACTCAATT TGTAGAACAA
ACCATAGCTA TAATGAAGAA CTTGCTAGAT AATCATACTG AAGGCAGCTC TGAACATCTA
GGGCAAGCTA GCATTGAAAC AATGATGTTA AATCTGGTCA GGTATGTTCG TGTGCTTGGG
AATATGGTCC ATGCAATTCA AATAAAAACG AAACTGTGTC AATTAGTTGA AGTAATGATG
GCAAGGAGAG ATGACCTCTC ATTTTGCCAA GAGATGAAAT TTAGGAATAA GATGGTAGAA
TACCTGACAG ACTGGGTTAT GGGAACATCA AACCAAGCAG CAGATGATGA TGTAAAATGT
CTTACAAGAG ATTTGGACCA GGCAAGCATG GAAGCAGTAG TTTCACTTCT AGCTGGTCTC
CCTCTGCAGC CTGAAGAAGG AGATGGTGTG GAATTGATGG AAGCCAAATC ACAGTTATTT
CTTAAATACT TCACATTATT TATGAACCTT TTGAATGACT GCAGTGAAGT TGAAGATGAA
AGTGCGCAAA CAGGTGGCAG GAAACGTGGC ATGTCTCGGA GGCTGGCATC ACTGAGGCAC
TGTACGGTCC TTGCAATGTC AAACTTACTC AATGCCAACG TAGACAGTGG TCTCATGCAC
TCCATAGGCT TAGGTTACCA CAAGGATCTC CAGACAAGAG CTACATTTAT GGAAGTTCTG
ACAAAAATCC TTCAACAAGG CACAGAATTT GACACACTTG CAGAAACAGT ATTGGCTGAT
CGGTTTGAGA GATTGGTGGA ACTGGTCACA ATGATGGGTG ATCAAGGAGA ACTCCCTATA
GCGATGGCTC TGGCCAATGT GGTTCCTTGT TCTCAGTGGG ATGAACTAGC TCGAGTTCTG
GTTACTCTGT TTGATTCTCG GCATTTACTC TACCAACTGC TCTGGAACAT GTTTTCTAAA
GAAGTAGAAT TGGCAGACTC CATGCAGACT CTCTTCCGAG GCAACAGCTT GGCCAGTAAA
ATAATGACAT TCTGTTTCAA GGTATATGGT GCTACCTATC TACAAAAACT CCTGGATCCT
TTATTACGAA TTGTGATCAC ATCCTCTGAT TGGCAACATG TTAGCTTTGA AGTGGATCCT
ACCAGGTTAG AACCATCAGA GAGCCTTGAG GAAAACCAGC GGAACCTCCT TCAGATGACT
GAAAAGTTCT TCCATGCCAT CATCAGTTCC TCCTCAGAAT TCCCCCCTCA ACTTCGAAGT
GTGTGCCACT GTTTATACCA GGCAACTTGC CACTCCCTAC TGAATAAAGC TACAGTAAAA
GAAAAAAAGG AAAACAAAAA ATCAGTGGTT AGCCAGCGTT TCCCTCAGAA CAGCATCGGT
GCAGTAGGAA GTGCCATGTT CCTCAGATTT ATCAATCCTG CCATTGTCTC ACCGTATGAA
GCAGGGATTT TAGATAAAAA GCCACCACCT AGAATCGAAA GGGGCTTGAA GTTAATGTCA
AAGATACTTC AGAGTATTGC CAATCATGTT CTCTTCACAA AAGAAGAACA TATGCGGCCT
TTCAATGATT TTGTGAAAAG CAACTTTGAT GCAGCACGCA GGTTTTTCCT TGATATAGCA
TCTGATTGTC CTACAAGTGA TGCAGTAAAT CATAGTCTTT CCTTCATAAG TGACGGCAAT
GTGCTTGCTT TACATCGTCT ACTCTGGAAC AATCAGGAGA AAATTGGGCA GTATCTTTCC
AGCAACAGGG ATCATAAAGC TGTTGGAAGA CGACCTTTTG ATAAGATGGC AACACTTCTT
GCATACCTGG GTCCTCCAGA GCACAAACCT GTGGCAGATA CACACTGGTC CAGCCTTAAC
CTTACCAGTT CAAAGTTTGA GGAATTTATG ACTAGGCATC AGGTACATGA AAAAGAAGAA
TTCAAGGCTT TGAAAACGTT AAGTATTTTC TACCAAGCTG GGACTTCCAA AGCTGGGAAT
CCTATTTTTT ATTATGTTGC ACGGAGGTTC AAAACTGGTC AAATCAATGG TGATTTGCTG
ATATACCATG TCTTACTGAC TTTAAAGCCA TATTATGCAA AGCCATATGA AATTGTAGTG
GACCTTACCC ATACCGGGCC TAGCAATCGC TTTAAAACAG ACTTTCTCTC TAAGTGGTTT
GTTGTTTTTC CTGGCTTTGC TTACGACAAC GTCTCCGCAG TCTATATCTA TAACTGTAAC
TCCTGGGTCA GGGAGTACAC CAAGTATCAT GAGCGGCTGC TGACTGGCCT CAAAGGTAGC
AAAAGGCTTG TTTTCATAGA CTGTCCTGGG AAACTGGCTG AGCACATAGA GCATGAACAA
CAGAAACTAC CTGCTGCCAC CTTGGCTTTA GAAGAGGACC TGAAGGTATT CCACAATGCT
CTCAAGCTAG CTCACAAAGA CACCAAAGTT TCTATTAAAG TTGGTTCTAC TGCTGTCCAA
GTAACTTCAG CAGAGCGAAC AAAAGTCCTA GGGCAATCAG TCTTTCTAAA TGACATTTAT
TATGCTTCGG AAATTGAAGA AATCTGCCTA GTAGATGAGA ACCAGTTCAC CTTAACCATT
GCAAACCAGG GCACGCCGCT CACCTTCATG CACCAGGAGT GTGAAGCCAT TGTCCAGTCT
ATCATTCATA TCCGGACCCG CTGGGAACTG TCACAGCCCG ACTCTATCCC CCAACACACC
AAGATTCGGC CAAAAGATGT CCCTGGGACA CTGCTCAATA TCGCATTACT TAATTTAGGC
AGTTCTGACC CGAGTTTACG GTCAGCTGCC TATAATCTTC TGTGTGCCTT AACTTGTACC
TTTAATTTAA AAATCGAGGG CCAGTTACTA GAGACATCAG GTTTATGTAT CCCTGCCAAC
AACACCCTCT TTATTGTCTC TATTAGTAAG ACACTGGCAG CCAATGAGCC ACACCTCACG
TTAGAATTTT TGGAAGAGTG TATTTCTGGA TTTAGCAAAT CTAGTATTGA ATTGAAACAC
CTTTGTTTGG AATACATGAC TCCATGGCTG TCAAATCTAG TTCGTTTTTG CAAGCATAAT
GATGATGCCA AACGACAAAG AGTTACTGCT ATTCTTGACA AGCTGATAAC AATGACCATC
AATGAAAAAC AGATGTACCC ATCTATTCAA GCAAAAATAT GGGGAAGCCT TGGGCAGATT
ACAGATCTGC TTGATGTTGT ACTAGACAGT TTCATCAAAA CCAGTGCAAC AGGTGGCTTG
GGATCAATAA AAGCTGAGGT GATGGCAGAT ACTGCTGTAG CTTTGGCTTC TGGAAATGTG
AAATTGGTTT CAAGCAAGGT TATTGGAAGG ATGTGCAAAA TAATTGACAA GACATGCTTA
TCTCCAACTC CTACTTTAGA ACAACATCTT ATGTGGGATG ATATTGCTAT TTTAGCACGC
TACATGCTGA TGCTGTCCTT CAACAATTCC CTTGATGTGG CAGCTCATCT TCCCTACCTC
TTCCACGTTG TTACTTTCTT AGTAGCCACA GGTCCGCTCT CCCTTAGAGC TTCCACACAT
GGACTGGTCA TTAATATCAT TCACTCTCTG TGTACTTGTT CACAGCTTCA TTTTAGTGAA
GAGACCAAGC AAGTTTTGAG ACTCAGTCTG ACAGAGTTCT CATTACCCAA ATTTTACTTG
CTGTTTGGCA TTAGCAAAGT CAAGTCAGCT GCTGTCATTG CCTTCCGTTC CAGTTACCGG
GACAGGTCAT TCTCTCCTGG CTCCTATGAG AGAGAGACTT TTGCTTTGAC ATCCTTGGAA
ACAGTCACAG AAGCTTTGTT GGAGATCATG GAGGCATGCA TGAGAGATAT TCCAACGTGC
AAGTGGCTGG ACCAGTGGAC AGAACTAGCT CAAAGATTTG CATTCCAATA TAATCCATCC
CTGCAACCAA GAGCTCTTGT TGTCTTTGGG TGTATTAGCA AACGAGTGTC TCATGGGCAG
ATAAAGCAGA TAATCCGTAT TCTTAGCAAG GCACTTGAGA GTTGCTTAAA AGGACCTGAC
ACTTACAACA GTCAAGTTCT GATAGAAGCT ACAGTAATAG CACTAACCAA ATTACAGCCA
CTTCTTAATA AGGACTCGCC TCTGCACAAA GCCCTCTTTT GGGTAGCTGT GGCTGTGCTG
CAGCTTGATG AGGTCAACTT GTATTCAGCA GGTACCGCAC TTCTTGAACA AAACCTGCAT
ACTTTAGATA GTCTCCGTAT ATTCAATGAC AAGAGTCCAG AGGAAGTATT TATGGCAATC
CGGAATCCTC TGGAGTGGCA CTGCAAGCAA ATGGATCATT TTGTTGGACT CAATTTCAAC
TCTAACTTTA ACTTTGCATT GGTTGGACAC CTTTTAAAAG GGTACAGGCA TCCTTCACCT
GCTATTGTTG CAAGAACAGT CAGAATTTTA CATACACTAC TAACTCTGGT TAACAAACAC
AGAAATTGTG ACAAATTTGA AGTGAATACA CAGAGCGTGG CCTACTTAGC AGCTTTACTT
ACAGTGTCTG AAGAAGTTCG AAGTCGCTGC AGCCTAAAAC ATAGAAAGTC ACTTCTTCTT
ACTGATATTT CAATGGAAAA TGTTCCTATG GATACATATC CCATTCATCA TGGTGACCCT
TCCTATAGGA CACTAAAGGA GACTCAGCCA TGGTCCTCTC CCAAAGGTTC TGAAGGATAC
CTTGCAGCCA CCTATCCAAC TGTCGGCCAG ACCAGTCCCC GAGCCAGGAA ATCCATGAGC
CTGGACATGG GGCAACCTTC TCAGGCCAAC ACTAAGAAGT TGCTTGGAAC AAGGAAAAGT
TTTGATCACT TGATATCAGA CACAAAGGCT CCTAAAAGGC AAGAAATGGA ATCAGGGATC
ACAACACCCC CCAAAATGAG GAGAGTAGCA GAAACTGATT ATGAAATGGA AACTCAGAGG
ATTTCCTCAT CACAACAGCA CCCACATTTA CGTAAAGTTT CAGTGTCTGA ATCAAATGTT
CTCTTGGATG AAGAAGTACT TACTGATCCG AAGATCCAGG CGCTGCTTCT TACTGTTCTA
GCTACACTGG TAAAATATAC CACAGATGAG TTTGATCAAC GAATTCTTTA TGAATACTTA
GCAGAGGCCA GTGTTGTGTT TCCCAAAGTC TTTCCTGTTG TGCATAATTT GTTGGACTCT
AAGATCAACA CCCTGTTATC ATTGTGCCAA GATCCAAATT TGTTAAATCC AATCCATGGA
ATTGTGCAGA GTGTGGTGTA CCATGAAGAA TCCCCACCAC AATACCAAAC ATCTTACCTG
CAAAGTTTTG GTTTTAATGG CTTGTGGCGG TTTGCAGGAC CGTTTTCAAA GCAAACACAA
ATTCCAGACT ATGCTGAGCT TATTGTTAAG TTTCTTGATG CCTTGATTGA CACGTACCTG
CCTGGAATTG ATGAAGAAAC CAGTGAAGAA TCCCTCCTGA CTCCCACATC TCCTTACCCT
CCTGCACTGC AGAGCCAGCT TAGTATCACT GCCAACCTTA ACCTTTCTAA TTCCATGACC
TCACTTGCAA CTTCCCAGCA TTCCCCAGCT TCTCTGCCTT GCTCTAAATC AGCAGTTTTC
ATGCAGCTGT TCCCTCATCA AGGAATCGAC AAGGAGAACG TTGAACTCTC CCCTACCACT
GGCCACTGTA ACAGTGGACG AACTCGCCAC GGATCCGCAA GCCAAGTGCA GAAGCAAAGA
AGCGCTGGCA GTTTCAAACG TAATAGCATT AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50429
Clone ID Related Accession (Same CDS sequence) XM_006721923 , XM_011524853 , XM_011524854 , XM_011524855 , XM_011524856
Accession Version XM_011524854.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8565bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neurofibromin isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
ATGTGGAATG CAAAGGAGAG GCTTCCAATA AAAACAGGAC AGCAGAACAC ACATACCAAA 
GTCAGTACTG AGCACAACAA GGAATGTCTA ATCAATATTT CCAAATACAA GTTTTCTTTG
GTTATAAGCG GCCTCACTAC TATTTTAAAG AATGTTAACA ATATGAGAAT ATTTGGAGAA
GCTGCTGAAA AAAATTTATA TCTCTCTCAG TTGATTATAT TGGATACACT GGAAAAATGT
CTTGCTGGGC AACCAAAGGA CACAATGAGA TTAGATGAAA CGATGCTGGT CAAACAGTTG
CTGCCAGAAA TCTGCCATTT TCTTCACACC TGTCGTGAAG GAAACCAGCA TGCAGCTGAA
CTTCGGAATT CTGCCTCTGG GGTTTTATTT TCTCTCAGCT GCAACAACTT CAATGCAGTC
TTTAGTCGCA TTTCTACCAG GTTACAGGAA TTAACTGTTT GTTCAGAAGA CAATGTTGAT
GTTCATGATA TAGAATTGTT ACAGTATATC AATGTGGATT GTGCAAAATT AAAACGACTC
CTGAAGGAAA CAGCATTTAA ATTTAAAGCC CTAAAGAAGG TTGCGCAGTT AGCAGTTATA
AATAGCCTGG AAAAGGCATT TTGGAACTGG GTAGAAAATT ATCCAGATGA ATTTACAAAA
CTGTACCAGA TCCCACAGAC TGATATGGCT GAATGTGCAG AAAAGCTATT TGACTTGGTG
GATGGTTTTG CTGAAAGCAC CAAACGTAAA GCAGCAGTTT GGCCACTACA AATCATTCTC
CTTATCTTGT GTCCAGAAAT AATCCAGGAT ATATCCAAAG ACGTGGTTGA TGAAAACAAC
ATGAATAAGA AGTTATTTCT GGACAGTCTA CGAAAAGCTC TTGCTGGCCA TGGAGGAAGT
AGGCAGCTGA CAGAAAGTGC TGCAATTGCC TGTGTCAAAC TGTGTAAAGC AAGTACTTAC
ATCAATTGGG AAGATAACTC TGTCATTTTC CTACTTGTTC AGTCCATGGT GGTTGATCTT
AAGAACCTGC TTTTTAATCC AAGTAAGCCA TTCTCAAGAG GCAGTCAGCC TGCAGATGTG
GATCTAATGA TTGACTGCCT TGTTTCTTGC TTTCGTATAA GCCCTCACAA CAACCAACAC
TTTAAGATCT GCCTGGCTCA GAATTCACCT TCTACATTTC ACTATGTGCT GGTAAATTCA
CTCCATCGAA TCATCACCAA TAGCACTTTC AAGCATGGAC TTGGCACTGC TTCCGCATTG
GATTGGTGGC CTAAGATTGA TGCTGTGTAT TGTCACTCGG TTGAACTTCG AAATATGTTT
GGTGAAACAC TTCATAAAGC AGTGCAAGGT TGTGGAGCAC ACCCAGCAAT ACGAATGGCA
CCGAGTCTTA CATTTAAAGA AAAAGTAACA AGCCTTAAAT TTAAAGAAAA ACCTACAGAC
CTGGAGACAA GAAGCTATAA GTATCTTCTC TTGTCCATGG TGAAACTAAT TCATGCAGAT
CCAAAGCTCT TGCTTTGTAA TCCAAGAAAA CAGGGGCCCG AAACCCAAGG CAGTACAGCA
GAATTAATTA CAGGGCTCGT CCAACTGGTC CCTCAGTCAC ACATGCCAGA GATTGCTCAG
GAAGCAATGG AGGCTCTGCT GGTTCTTCAT CAGTTAGATA GCATTGATTT GTGGAATCCT
GATGCTCCTG TAGAAACATT TTGGGAGATT AGCTCACAAA TGCTTTTTTA CATCTGCAAG
AAATTAACTA GTCATCAAAT GCTTAGTAGC ACAGAAATTC TCAAGTGGTT GCGGGAAATA
TTGATCTGCA GGAATAAATT TCTTCTTAAA AATAAGCAGG CAGATAGAAG TTCCTGTCAC
TTTCTCCTTT TTTACGGGGT AGGATGTGAT ATTCCTTCTA GTGGAAATAC CAGTCAAATG
TCCATGGATC ATGAAGAATT ACTACGTACT CCTGGAGCCT CTCTCCGGAA GGGAAAAGGG
AACTCCTCTA TGGATAGTGC AGCAGGATGC AGCGGAACCC CCCCGATTTG CCGACAAGCC
CAGACCAAAC TAGAAGTGGC CCTGTACATG TTTCTGTGGA ACCCTGACAC TGAAGCTGTT
CTGGTTGCCA TGTCCTGTTT CCGCCACCTC TGTGAGGAAG CAGATATCCG GTGTGGGGTG
GATGAAGTGT CAGTGCATAA CCTCTTGCCC AACTATAACA CATTCATGGA GTTTGCCTCT
GTCAGCAATA TGATGTCAAC AGGAAGAGCA GCACTTCAGA AAAGAGTGAT GGCACTGCTG
AGGCGCATTG AGCATCCCAC TGCAGGAAAC ACTGAGGCTT GGGAAGATAC ACATGCAAAA
TGGGAACAAG CAACAAAGCT AATCCTTAAC TATCCAAAAG CCAAAATGGA AGATGGCCAG
GCTGCTGAAA GCCTTCACAA GACCATTGTT AAGAGGCGAA TGTCCCATGT GAGTGGAGGA
GGATCCATAG ATTTGTCTGA CACAGACTCC CTACAGGAAT GGATCAACAT GACTGGCTTC
CTTTGTGCCC TTGGGGGAGT GTGCCTCCAG CAGAGAAGCA ATTCTGGCCT GGCAACCTAT
AGCCCACCCA TGGGTCCAGT CAGTGAACGT AAGGGTTCTA TGATTTCAGT GATGTCTTCA
GAGGGAAACG CAGATACACC TGTCAGCAAA TTTATGGATC GGCTGTTGTC CTTAATGGTG
TGTAACCATG AGAAAGTGGG ACTTCAAATA CGGACCAATG TTAAGGATCT GGTGGGTCTA
GAATTGAGTC CTGCTCTGTA TCCAATGCTA TTTAACAAAT TGAAGAATAC CATCAGCAAG
TTTTTTGACT CCCAAGGACA GGTTTTATTG ACTGATACCA ATACTCAATT TGTAGAACAA
ACCATAGCTA TAATGAAGAA CTTGCTAGAT AATCATACTG AAGGCAGCTC TGAACATCTA
GGGCAAGCTA GCATTGAAAC AATGATGTTA AATCTGGTCA GGTATGTTCG TGTGCTTGGG
AATATGGTCC ATGCAATTCA AATAAAAACG AAACTGTGTC AATTAGTTGA AGTAATGATG
GCAAGGAGAG ATGACCTCTC ATTTTGCCAA GAGATGAAAT TTAGGAATAA GATGGTAGAA
TACCTGACAG ACTGGGTTAT GGGAACATCA AACCAAGCAG CAGATGATGA TGTAAAATGT
CTTACAAGAG ATTTGGACCA GGCAAGCATG GAAGCAGTAG TTTCACTTCT AGCTGGTCTC
CCTCTGCAGC CTGAAGAAGG AGATGGTGTG GAATTGATGG AAGCCAAATC ACAGTTATTT
CTTAAATACT TCACATTATT TATGAACCTT TTGAATGACT GCAGTGAAGT TGAAGATGAA
AGTGCGCAAA CAGGTGGCAG GAAACGTGGC ATGTCTCGGA GGCTGGCATC ACTGAGGCAC
TGTACGGTCC TTGCAATGTC AAACTTACTC AATGCCAACG TAGACAGTGG TCTCATGCAC
TCCATAGGCT TAGGTTACCA CAAGGATCTC CAGACAAGAG CTACATTTAT GGAAGTTCTG
ACAAAAATCC TTCAACAAGG CACAGAATTT GACACACTTG CAGAAACAGT ATTGGCTGAT
CGGTTTGAGA GATTGGTGGA ACTGGTCACA ATGATGGGTG ATCAAGGAGA ACTCCCTATA
GCGATGGCTC TGGCCAATGT GGTTCCTTGT TCTCAGTGGG ATGAACTAGC TCGAGTTCTG
GTTACTCTGT TTGATTCTCG GCATTTACTC TACCAACTGC TCTGGAACAT GTTTTCTAAA
GAAGTAGAAT TGGCAGACTC CATGCAGACT CTCTTCCGAG GCAACAGCTT GGCCAGTAAA
ATAATGACAT TCTGTTTCAA GGTATATGGT GCTACCTATC TACAAAAACT CCTGGATCCT
TTATTACGAA TTGTGATCAC ATCCTCTGAT TGGCAACATG TTAGCTTTGA AGTGGATCCT
ACCAGGTTAG AACCATCAGA GAGCCTTGAG GAAAACCAGC GGAACCTCCT TCAGATGACT
GAAAAGTTCT TCCATGCCAT CATCAGTTCC TCCTCAGAAT TCCCCCCTCA ACTTCGAAGT
GTGTGCCACT GTTTATACCA GGCAACTTGC CACTCCCTAC TGAATAAAGC TACAGTAAAA
GAAAAAAAGG AAAACAAAAA ATCAGTGGTT AGCCAGCGTT TCCCTCAGAA CAGCATCGGT
GCAGTAGGAA GTGCCATGTT CCTCAGATTT ATCAATCCTG CCATTGTCTC ACCGTATGAA
GCAGGGATTT TAGATAAAAA GCCACCACCT AGAATCGAAA GGGGCTTGAA GTTAATGTCA
AAGATACTTC AGAGTATTGC CAATCATGTT CTCTTCACAA AAGAAGAACA TATGCGGCCT
TTCAATGATT TTGTGAAAAG CAACTTTGAT GCAGCACGCA GGTTTTTCCT TGATATAGCA
TCTGATTGTC CTACAAGTGA TGCAGTAAAT CATAGTCTTT CCTTCATAAG TGACGGCAAT
GTGCTTGCTT TACATCGTCT ACTCTGGAAC AATCAGGAGA AAATTGGGCA GTATCTTTCC
AGCAACAGGG ATCATAAAGC TGTTGGAAGA CGACCTTTTG ATAAGATGGC AACACTTCTT
GCATACCTGG GTCCTCCAGA GCACAAACCT GTGGCAGATA CACACTGGTC CAGCCTTAAC
CTTACCAGTT CAAAGTTTGA GGAATTTATG ACTAGGCATC AGGTACATGA AAAAGAAGAA
TTCAAGGCTT TGAAAACGTT AAGTATTTTC TACCAAGCTG GGACTTCCAA AGCTGGGAAT
CCTATTTTTT ATTATGTTGC ACGGAGGTTC AAAACTGGTC AAATCAATGG TGATTTGCTG
ATATACCATG TCTTACTGAC TTTAAAGCCA TATTATGCAA AGCCATATGA AATTGTAGTG
GACCTTACCC ATACCGGGCC TAGCAATCGC TTTAAAACAG ACTTTCTCTC TAAGTGGTTT
GTTGTTTTTC CTGGCTTTGC TTACGACAAC GTCTCCGCAG TCTATATCTA TAACTGTAAC
TCCTGGGTCA GGGAGTACAC CAAGTATCAT GAGCGGCTGC TGACTGGCCT CAAAGGTAGC
AAAAGGCTTG TTTTCATAGA CTGTCCTGGG AAACTGGCTG AGCACATAGA GCATGAACAA
CAGAAACTAC CTGCTGCCAC CTTGGCTTTA GAAGAGGACC TGAAGGTATT CCACAATGCT
CTCAAGCTAG CTCACAAAGA CACCAAAGTT TCTATTAAAG TTGGTTCTAC TGCTGTCCAA
GTAACTTCAG CAGAGCGAAC AAAAGTCCTA GGGCAATCAG TCTTTCTAAA TGACATTTAT
TATGCTTCGG AAATTGAAGA AATCTGCCTA GTAGATGAGA ACCAGTTCAC CTTAACCATT
GCAAACCAGG GCACGCCGCT CACCTTCATG CACCAGGAGT GTGAAGCCAT TGTCCAGTCT
ATCATTCATA TCCGGACCCG CTGGGAACTG TCACAGCCCG ACTCTATCCC CCAACACACC
AAGATTCGGC CAAAAGATGT CCCTGGGACA CTGCTCAATA TCGCATTACT TAATTTAGGC
AGTTCTGACC CGAGTTTACG GTCAGCTGCC TATAATCTTC TGTGTGCCTT AACTTGTACC
TTTAATTTAA AAATCGAGGG CCAGTTACTA GAGACATCAG GTTTATGTAT CCCTGCCAAC
AACACCCTCT TTATTGTCTC TATTAGTAAG ACACTGGCAG CCAATGAGCC ACACCTCACG
TTAGAATTTT TGGAAGAGTG TATTTCTGGA TTTAGCAAAT CTAGTATTGA ATTGAAACAC
CTTTGTTTGG AATACATGAC TCCATGGCTG TCAAATCTAG TTCGTTTTTG CAAGCATAAT
GATGATGCCA AACGACAAAG AGTTACTGCT ATTCTTGACA AGCTGATAAC AATGACCATC
AATGAAAAAC AGATGTACCC ATCTATTCAA GCAAAAATAT GGGGAAGCCT TGGGCAGATT
ACAGATCTGC TTGATGTTGT ACTAGACAGT TTCATCAAAA CCAGTGCAAC AGGTGGCTTG
GGATCAATAA AAGCTGAGGT GATGGCAGAT ACTGCTGTAG CTTTGGCTTC TGGAAATGTG
AAATTGGTTT CAAGCAAGGT TATTGGAAGG ATGTGCAAAA TAATTGACAA GACATGCTTA
TCTCCAACTC CTACTTTAGA ACAACATCTT ATGTGGGATG ATATTGCTAT TTTAGCACGC
TACATGCTGA TGCTGTCCTT CAACAATTCC CTTGATGTGG CAGCTCATCT TCCCTACCTC
TTCCACGTTG TTACTTTCTT AGTAGCCACA GGTCCGCTCT CCCTTAGAGC TTCCACACAT
GGACTGGTCA TTAATATCAT TCACTCTCTG TGTACTTGTT CACAGCTTCA TTTTAGTGAA
GAGACCAAGC AAGTTTTGAG ACTCAGTCTG ACAGAGTTCT CATTACCCAA ATTTTACTTG
CTGTTTGGCA TTAGCAAAGT CAAGTCAGCT GCTGTCATTG CCTTCCGTTC CAGTTACCGG
GACAGGTCAT TCTCTCCTGG CTCCTATGAG AGAGAGACTT TTGCTTTGAC ATCCTTGGAA
ACAGTCACAG AAGCTTTGTT GGAGATCATG GAGGCATGCA TGAGAGATAT TCCAACGTGC
AAGTGGCTGG ACCAGTGGAC AGAACTAGCT CAAAGATTTG CATTCCAATA TAATCCATCC
CTGCAACCAA GAGCTCTTGT TGTCTTTGGG TGTATTAGCA AACGAGTGTC TCATGGGCAG
ATAAAGCAGA TAATCCGTAT TCTTAGCAAG GCACTTGAGA GTTGCTTAAA AGGACCTGAC
ACTTACAACA GTCAAGTTCT GATAGAAGCT ACAGTAATAG CACTAACCAA ATTACAGCCA
CTTCTTAATA AGGACTCGCC TCTGCACAAA GCCCTCTTTT GGGTAGCTGT GGCTGTGCTG
CAGCTTGATG AGGTCAACTT GTATTCAGCA GGTACCGCAC TTCTTGAACA AAACCTGCAT
ACTTTAGATA GTCTCCGTAT ATTCAATGAC AAGAGTCCAG AGGAAGTATT TATGGCAATC
CGGAATCCTC TGGAGTGGCA CTGCAAGCAA ATGGATCATT TTGTTGGACT CAATTTCAAC
TCTAACTTTA ACTTTGCATT GGTTGGACAC CTTTTAAAAG GGTACAGGCA TCCTTCACCT
GCTATTGTTG CAAGAACAGT CAGAATTTTA CATACACTAC TAACTCTGGT TAACAAACAC
AGAAATTGTG ACAAATTTGA AGTGAATACA CAGAGCGTGG CCTACTTAGC AGCTTTACTT
ACAGTGTCTG AAGAAGTTCG AAGTCGCTGC AGCCTAAAAC ATAGAAAGTC ACTTCTTCTT
ACTGATATTT CAATGGAAAA TGTTCCTATG GATACATATC CCATTCATCA TGGTGACCCT
TCCTATAGGA CACTAAAGGA GACTCAGCCA TGGTCCTCTC CCAAAGGTTC TGAAGGATAC
CTTGCAGCCA CCTATCCAAC TGTCGGCCAG ACCAGTCCCC GAGCCAGGAA ATCCATGAGC
CTGGACATGG GGCAACCTTC TCAGGCCAAC ACTAAGAAGT TGCTTGGAAC AAGGAAAAGT
TTTGATCACT TGATATCAGA CACAAAGGCT CCTAAAAGGC AAGAAATGGA ATCAGGGATC
ACAACACCCC CCAAAATGAG GAGAGTAGCA GAAACTGATT ATGAAATGGA AACTCAGAGG
ATTTCCTCAT CACAACAGCA CCCACATTTA CGTAAAGTTT CAGTGTCTGA ATCAAATGTT
CTCTTGGATG AAGAAGTACT TACTGATCCG AAGATCCAGG CGCTGCTTCT TACTGTTCTA
GCTACACTGG TAAAATATAC CACAGATGAG TTTGATCAAC GAATTCTTTA TGAATACTTA
GCAGAGGCCA GTGTTGTGTT TCCCAAAGTC TTTCCTGTTG TGCATAATTT GTTGGACTCT
AAGATCAACA CCCTGTTATC ATTGTGCCAA GATCCAAATT TGTTAAATCC AATCCATGGA
ATTGTGCAGA GTGTGGTGTA CCATGAAGAA TCCCCACCAC AATACCAAAC ATCTTACCTG
CAAAGTTTTG GTTTTAATGG CTTGTGGCGG TTTGCAGGAC CGTTTTCAAA GCAAACACAA
ATTCCAGACT ATGCTGAGCT TATTGTTAAG TTTCTTGATG CCTTGATTGA CACGTACCTG
CCTGGAATTG ATGAAGAAAC CAGTGAAGAA TCCCTCCTGA CTCCCACATC TCCTTACCCT
CCTGCACTGC AGAGCCAGCT TAGTATCACT GCCAACCTTA ACCTTTCTAA TTCCATGACC
TCACTTGCAA CTTCCCAGCA TTCCCCAGCT TCTCTGCCTT GCTCTAAATC AGCAGTTTTC
ATGCAGCTGT TCCCTCATCA AGGAATCGAC AAGGAGAACG TTGAACTCTC CCCTACCACT
GGCCACTGTA ACAGTGGACG AACTCGCCAC GGATCCGCAA GCCAAGTGCA GAAGCAAAGA
AGCGCTGGCA GTTTCAAACG TAATAGCATT AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523156.1
CDS866..9430
Misc Feature(1)4415..5527(+)
Misc Feature(2)4448..5509(+)
Misc Feature(3)4562..5230(+)
Misc Feature(4)5597..6040(+)
Misc Feature(5)5666..5971(+)
Misc Feature(6)5870..5968(+)
Misc Feature(7)6038..6367(+)
Misc Feature(8)6038..6367(+)
Translation

Target ORF information:

RefSeq Version XM_011524854
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524854

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
ATGTGGAATG CAAAGGAGAG GCTTCCAATA AAAACAGGAC AGCAGAACAC ACATACCAAA 
GTCAGTACTG AGCACAACAA GGAATGTCTA ATCAATATTT CCAAATACAA GTTTTCTTTG
GTTATAAGCG GCCTCACTAC TATTTTAAAG AATGTTAACA ATATGAGAAT ATTTGGAGAA
GCTGCTGAAA AAAATTTATA TCTCTCTCAG TTGATTATAT TGGATACACT GGAAAAATGT
CTTGCTGGGC AACCAAAGGA CACAATGAGA TTAGATGAAA CGATGCTGGT CAAACAGTTG
CTGCCAGAAA TCTGCCATTT TCTTCACACC TGTCGTGAAG GAAACCAGCA TGCAGCTGAA
CTTCGGAATT CTGCCTCTGG GGTTTTATTT TCTCTCAGCT GCAACAACTT CAATGCAGTC
TTTAGTCGCA TTTCTACCAG GTTACAGGAA TTAACTGTTT GTTCAGAAGA CAATGTTGAT
GTTCATGATA TAGAATTGTT ACAGTATATC AATGTGGATT GTGCAAAATT AAAACGACTC
CTGAAGGAAA CAGCATTTAA ATTTAAAGCC CTAAAGAAGG TTGCGCAGTT AGCAGTTATA
AATAGCCTGG AAAAGGCATT TTGGAACTGG GTAGAAAATT ATCCAGATGA ATTTACAAAA
CTGTACCAGA TCCCACAGAC TGATATGGCT GAATGTGCAG AAAAGCTATT TGACTTGGTG
GATGGTTTTG CTGAAAGCAC CAAACGTAAA GCAGCAGTTT GGCCACTACA AATCATTCTC
CTTATCTTGT GTCCAGAAAT AATCCAGGAT ATATCCAAAG ACGTGGTTGA TGAAAACAAC
ATGAATAAGA AGTTATTTCT GGACAGTCTA CGAAAAGCTC TTGCTGGCCA TGGAGGAAGT
AGGCAGCTGA CAGAAAGTGC TGCAATTGCC TGTGTCAAAC TGTGTAAAGC AAGTACTTAC
ATCAATTGGG AAGATAACTC TGTCATTTTC CTACTTGTTC AGTCCATGGT GGTTGATCTT
AAGAACCTGC TTTTTAATCC AAGTAAGCCA TTCTCAAGAG GCAGTCAGCC TGCAGATGTG
GATCTAATGA TTGACTGCCT TGTTTCTTGC TTTCGTATAA GCCCTCACAA CAACCAACAC
TTTAAGATCT GCCTGGCTCA GAATTCACCT TCTACATTTC ACTATGTGCT GGTAAATTCA
CTCCATCGAA TCATCACCAA TAGCACTTTC AAGCATGGAC TTGGCACTGC TTCCGCATTG
GATTGGTGGC CTAAGATTGA TGCTGTGTAT TGTCACTCGG TTGAACTTCG AAATATGTTT
GGTGAAACAC TTCATAAAGC AGTGCAAGGT TGTGGAGCAC ACCCAGCAAT ACGAATGGCA
CCGAGTCTTA CATTTAAAGA AAAAGTAACA AGCCTTAAAT TTAAAGAAAA ACCTACAGAC
CTGGAGACAA GAAGCTATAA GTATCTTCTC TTGTCCATGG TGAAACTAAT TCATGCAGAT
CCAAAGCTCT TGCTTTGTAA TCCAAGAAAA CAGGGGCCCG AAACCCAAGG CAGTACAGCA
GAATTAATTA CAGGGCTCGT CCAACTGGTC CCTCAGTCAC ACATGCCAGA GATTGCTCAG
GAAGCAATGG AGGCTCTGCT GGTTCTTCAT CAGTTAGATA GCATTGATTT GTGGAATCCT
GATGCTCCTG TAGAAACATT TTGGGAGATT AGCTCACAAA TGCTTTTTTA CATCTGCAAG
AAATTAACTA GTCATCAAAT GCTTAGTAGC ACAGAAATTC TCAAGTGGTT GCGGGAAATA
TTGATCTGCA GGAATAAATT TCTTCTTAAA AATAAGCAGG CAGATAGAAG TTCCTGTCAC
TTTCTCCTTT TTTACGGGGT AGGATGTGAT ATTCCTTCTA GTGGAAATAC CAGTCAAATG
TCCATGGATC ATGAAGAATT ACTACGTACT CCTGGAGCCT CTCTCCGGAA GGGAAAAGGG
AACTCCTCTA TGGATAGTGC AGCAGGATGC AGCGGAACCC CCCCGATTTG CCGACAAGCC
CAGACCAAAC TAGAAGTGGC CCTGTACATG TTTCTGTGGA ACCCTGACAC TGAAGCTGTT
CTGGTTGCCA TGTCCTGTTT CCGCCACCTC TGTGAGGAAG CAGATATCCG GTGTGGGGTG
GATGAAGTGT CAGTGCATAA CCTCTTGCCC AACTATAACA CATTCATGGA GTTTGCCTCT
GTCAGCAATA TGATGTCAAC AGGAAGAGCA GCACTTCAGA AAAGAGTGAT GGCACTGCTG
AGGCGCATTG AGCATCCCAC TGCAGGAAAC ACTGAGGCTT GGGAAGATAC ACATGCAAAA
TGGGAACAAG CAACAAAGCT AATCCTTAAC TATCCAAAAG CCAAAATGGA AGATGGCCAG
GCTGCTGAAA GCCTTCACAA GACCATTGTT AAGAGGCGAA TGTCCCATGT GAGTGGAGGA
GGATCCATAG ATTTGTCTGA CACAGACTCC CTACAGGAAT GGATCAACAT GACTGGCTTC
CTTTGTGCCC TTGGGGGAGT GTGCCTCCAG CAGAGAAGCA ATTCTGGCCT GGCAACCTAT
AGCCCACCCA TGGGTCCAGT CAGTGAACGT AAGGGTTCTA TGATTTCAGT GATGTCTTCA
GAGGGAAACG CAGATACACC TGTCAGCAAA TTTATGGATC GGCTGTTGTC CTTAATGGTG
TGTAACCATG AGAAAGTGGG ACTTCAAATA CGGACCAATG TTAAGGATCT GGTGGGTCTA
GAATTGAGTC CTGCTCTGTA TCCAATGCTA TTTAACAAAT TGAAGAATAC CATCAGCAAG
TTTTTTGACT CCCAAGGACA GGTTTTATTG ACTGATACCA ATACTCAATT TGTAGAACAA
ACCATAGCTA TAATGAAGAA CTTGCTAGAT AATCATACTG AAGGCAGCTC TGAACATCTA
GGGCAAGCTA GCATTGAAAC AATGATGTTA AATCTGGTCA GGTATGTTCG TGTGCTTGGG
AATATGGTCC ATGCAATTCA AATAAAAACG AAACTGTGTC AATTAGTTGA AGTAATGATG
GCAAGGAGAG ATGACCTCTC ATTTTGCCAA GAGATGAAAT TTAGGAATAA GATGGTAGAA
TACCTGACAG ACTGGGTTAT GGGAACATCA AACCAAGCAG CAGATGATGA TGTAAAATGT
CTTACAAGAG ATTTGGACCA GGCAAGCATG GAAGCAGTAG TTTCACTTCT AGCTGGTCTC
CCTCTGCAGC CTGAAGAAGG AGATGGTGTG GAATTGATGG AAGCCAAATC ACAGTTATTT
CTTAAATACT TCACATTATT TATGAACCTT TTGAATGACT GCAGTGAAGT TGAAGATGAA
AGTGCGCAAA CAGGTGGCAG GAAACGTGGC ATGTCTCGGA GGCTGGCATC ACTGAGGCAC
TGTACGGTCC TTGCAATGTC AAACTTACTC AATGCCAACG TAGACAGTGG TCTCATGCAC
TCCATAGGCT TAGGTTACCA CAAGGATCTC CAGACAAGAG CTACATTTAT GGAAGTTCTG
ACAAAAATCC TTCAACAAGG CACAGAATTT GACACACTTG CAGAAACAGT ATTGGCTGAT
CGGTTTGAGA GATTGGTGGA ACTGGTCACA ATGATGGGTG ATCAAGGAGA ACTCCCTATA
GCGATGGCTC TGGCCAATGT GGTTCCTTGT TCTCAGTGGG ATGAACTAGC TCGAGTTCTG
GTTACTCTGT TTGATTCTCG GCATTTACTC TACCAACTGC TCTGGAACAT GTTTTCTAAA
GAAGTAGAAT TGGCAGACTC CATGCAGACT CTCTTCCGAG GCAACAGCTT GGCCAGTAAA
ATAATGACAT TCTGTTTCAA GGTATATGGT GCTACCTATC TACAAAAACT CCTGGATCCT
TTATTACGAA TTGTGATCAC ATCCTCTGAT TGGCAACATG TTAGCTTTGA AGTGGATCCT
ACCAGGTTAG AACCATCAGA GAGCCTTGAG GAAAACCAGC GGAACCTCCT TCAGATGACT
GAAAAGTTCT TCCATGCCAT CATCAGTTCC TCCTCAGAAT TCCCCCCTCA ACTTCGAAGT
GTGTGCCACT GTTTATACCA GGCAACTTGC CACTCCCTAC TGAATAAAGC TACAGTAAAA
GAAAAAAAGG AAAACAAAAA ATCAGTGGTT AGCCAGCGTT TCCCTCAGAA CAGCATCGGT
GCAGTAGGAA GTGCCATGTT CCTCAGATTT ATCAATCCTG CCATTGTCTC ACCGTATGAA
GCAGGGATTT TAGATAAAAA GCCACCACCT AGAATCGAAA GGGGCTTGAA GTTAATGTCA
AAGATACTTC AGAGTATTGC CAATCATGTT CTCTTCACAA AAGAAGAACA TATGCGGCCT
TTCAATGATT TTGTGAAAAG CAACTTTGAT GCAGCACGCA GGTTTTTCCT TGATATAGCA
TCTGATTGTC CTACAAGTGA TGCAGTAAAT CATAGTCTTT CCTTCATAAG TGACGGCAAT
GTGCTTGCTT TACATCGTCT ACTCTGGAAC AATCAGGAGA AAATTGGGCA GTATCTTTCC
AGCAACAGGG ATCATAAAGC TGTTGGAAGA CGACCTTTTG ATAAGATGGC AACACTTCTT
GCATACCTGG GTCCTCCAGA GCACAAACCT GTGGCAGATA CACACTGGTC CAGCCTTAAC
CTTACCAGTT CAAAGTTTGA GGAATTTATG ACTAGGCATC AGGTACATGA AAAAGAAGAA
TTCAAGGCTT TGAAAACGTT AAGTATTTTC TACCAAGCTG GGACTTCCAA AGCTGGGAAT
CCTATTTTTT ATTATGTTGC ACGGAGGTTC AAAACTGGTC AAATCAATGG TGATTTGCTG
ATATACCATG TCTTACTGAC TTTAAAGCCA TATTATGCAA AGCCATATGA AATTGTAGTG
GACCTTACCC ATACCGGGCC TAGCAATCGC TTTAAAACAG ACTTTCTCTC TAAGTGGTTT
GTTGTTTTTC CTGGCTTTGC TTACGACAAC GTCTCCGCAG TCTATATCTA TAACTGTAAC
TCCTGGGTCA GGGAGTACAC CAAGTATCAT GAGCGGCTGC TGACTGGCCT CAAAGGTAGC
AAAAGGCTTG TTTTCATAGA CTGTCCTGGG AAACTGGCTG AGCACATAGA GCATGAACAA
CAGAAACTAC CTGCTGCCAC CTTGGCTTTA GAAGAGGACC TGAAGGTATT CCACAATGCT
CTCAAGCTAG CTCACAAAGA CACCAAAGTT TCTATTAAAG TTGGTTCTAC TGCTGTCCAA
GTAACTTCAG CAGAGCGAAC AAAAGTCCTA GGGCAATCAG TCTTTCTAAA TGACATTTAT
TATGCTTCGG AAATTGAAGA AATCTGCCTA GTAGATGAGA ACCAGTTCAC CTTAACCATT
GCAAACCAGG GCACGCCGCT CACCTTCATG CACCAGGAGT GTGAAGCCAT TGTCCAGTCT
ATCATTCATA TCCGGACCCG CTGGGAACTG TCACAGCCCG ACTCTATCCC CCAACACACC
AAGATTCGGC CAAAAGATGT CCCTGGGACA CTGCTCAATA TCGCATTACT TAATTTAGGC
AGTTCTGACC CGAGTTTACG GTCAGCTGCC TATAATCTTC TGTGTGCCTT AACTTGTACC
TTTAATTTAA AAATCGAGGG CCAGTTACTA GAGACATCAG GTTTATGTAT CCCTGCCAAC
AACACCCTCT TTATTGTCTC TATTAGTAAG ACACTGGCAG CCAATGAGCC ACACCTCACG
TTAGAATTTT TGGAAGAGTG TATTTCTGGA TTTAGCAAAT CTAGTATTGA ATTGAAACAC
CTTTGTTTGG AATACATGAC TCCATGGCTG TCAAATCTAG TTCGTTTTTG CAAGCATAAT
GATGATGCCA AACGACAAAG AGTTACTGCT ATTCTTGACA AGCTGATAAC AATGACCATC
AATGAAAAAC AGATGTACCC ATCTATTCAA GCAAAAATAT GGGGAAGCCT TGGGCAGATT
ACAGATCTGC TTGATGTTGT ACTAGACAGT TTCATCAAAA CCAGTGCAAC AGGTGGCTTG
GGATCAATAA AAGCTGAGGT GATGGCAGAT ACTGCTGTAG CTTTGGCTTC TGGAAATGTG
AAATTGGTTT CAAGCAAGGT TATTGGAAGG ATGTGCAAAA TAATTGACAA GACATGCTTA
TCTCCAACTC CTACTTTAGA ACAACATCTT ATGTGGGATG ATATTGCTAT TTTAGCACGC
TACATGCTGA TGCTGTCCTT CAACAATTCC CTTGATGTGG CAGCTCATCT TCCCTACCTC
TTCCACGTTG TTACTTTCTT AGTAGCCACA GGTCCGCTCT CCCTTAGAGC TTCCACACAT
GGACTGGTCA TTAATATCAT TCACTCTCTG TGTACTTGTT CACAGCTTCA TTTTAGTGAA
GAGACCAAGC AAGTTTTGAG ACTCAGTCTG ACAGAGTTCT CATTACCCAA ATTTTACTTG
CTGTTTGGCA TTAGCAAAGT CAAGTCAGCT GCTGTCATTG CCTTCCGTTC CAGTTACCGG
GACAGGTCAT TCTCTCCTGG CTCCTATGAG AGAGAGACTT TTGCTTTGAC ATCCTTGGAA
ACAGTCACAG AAGCTTTGTT GGAGATCATG GAGGCATGCA TGAGAGATAT TCCAACGTGC
AAGTGGCTGG ACCAGTGGAC AGAACTAGCT CAAAGATTTG CATTCCAATA TAATCCATCC
CTGCAACCAA GAGCTCTTGT TGTCTTTGGG TGTATTAGCA AACGAGTGTC TCATGGGCAG
ATAAAGCAGA TAATCCGTAT TCTTAGCAAG GCACTTGAGA GTTGCTTAAA AGGACCTGAC
ACTTACAACA GTCAAGTTCT GATAGAAGCT ACAGTAATAG CACTAACCAA ATTACAGCCA
CTTCTTAATA AGGACTCGCC TCTGCACAAA GCCCTCTTTT GGGTAGCTGT GGCTGTGCTG
CAGCTTGATG AGGTCAACTT GTATTCAGCA GGTACCGCAC TTCTTGAACA AAACCTGCAT
ACTTTAGATA GTCTCCGTAT ATTCAATGAC AAGAGTCCAG AGGAAGTATT TATGGCAATC
CGGAATCCTC TGGAGTGGCA CTGCAAGCAA ATGGATCATT TTGTTGGACT CAATTTCAAC
TCTAACTTTA ACTTTGCATT GGTTGGACAC CTTTTAAAAG GGTACAGGCA TCCTTCACCT
GCTATTGTTG CAAGAACAGT CAGAATTTTA CATACACTAC TAACTCTGGT TAACAAACAC
AGAAATTGTG ACAAATTTGA AGTGAATACA CAGAGCGTGG CCTACTTAGC AGCTTTACTT
ACAGTGTCTG AAGAAGTTCG AAGTCGCTGC AGCCTAAAAC ATAGAAAGTC ACTTCTTCTT
ACTGATATTT CAATGGAAAA TGTTCCTATG GATACATATC CCATTCATCA TGGTGACCCT
TCCTATAGGA CACTAAAGGA GACTCAGCCA TGGTCCTCTC CCAAAGGTTC TGAAGGATAC
CTTGCAGCCA CCTATCCAAC TGTCGGCCAG ACCAGTCCCC GAGCCAGGAA ATCCATGAGC
CTGGACATGG GGCAACCTTC TCAGGCCAAC ACTAAGAAGT TGCTTGGAAC AAGGAAAAGT
TTTGATCACT TGATATCAGA CACAAAGGCT CCTAAAAGGC AAGAAATGGA ATCAGGGATC
ACAACACCCC CCAAAATGAG GAGAGTAGCA GAAACTGATT ATGAAATGGA AACTCAGAGG
ATTTCCTCAT CACAACAGCA CCCACATTTA CGTAAAGTTT CAGTGTCTGA ATCAAATGTT
CTCTTGGATG AAGAAGTACT TACTGATCCG AAGATCCAGG CGCTGCTTCT TACTGTTCTA
GCTACACTGG TAAAATATAC CACAGATGAG TTTGATCAAC GAATTCTTTA TGAATACTTA
GCAGAGGCCA GTGTTGTGTT TCCCAAAGTC TTTCCTGTTG TGCATAATTT GTTGGACTCT
AAGATCAACA CCCTGTTATC ATTGTGCCAA GATCCAAATT TGTTAAATCC AATCCATGGA
ATTGTGCAGA GTGTGGTGTA CCATGAAGAA TCCCCACCAC AATACCAAAC ATCTTACCTG
CAAAGTTTTG GTTTTAATGG CTTGTGGCGG TTTGCAGGAC CGTTTTCAAA GCAAACACAA
ATTCCAGACT ATGCTGAGCT TATTGTTAAG TTTCTTGATG CCTTGATTGA CACGTACCTG
CCTGGAATTG ATGAAGAAAC CAGTGAAGAA TCCCTCCTGA CTCCCACATC TCCTTACCCT
CCTGCACTGC AGAGCCAGCT TAGTATCACT GCCAACCTTA ACCTTTCTAA TTCCATGACC
TCACTTGCAA CTTCCCAGCA TTCCCCAGCT TCTCTGCCTT GCTCTAAATC AGCAGTTTTC
ATGCAGCTGT TCCCTCATCA AGGAATCGAC AAGGAGAACG TTGAACTCTC CCCTACCACT
GGCCACTGTA ACAGTGGACG AACTCGCCAC GGATCCGCAA GCCAAGTGCA GAAGCAAAGA
AGCGCTGGCA GTTTCAAACG TAATAGCATT AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50429
Clone ID Related Accession (Same CDS sequence) XM_006721923 , XM_011524853 , XM_011524854 , XM_011524855 , XM_011524856
Accession Version XM_011524855.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8565bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neurofibromin isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
ATGTGGAATG CAAAGGAGAG GCTTCCAATA AAAACAGGAC AGCAGAACAC ACATACCAAA 
GTCAGTACTG AGCACAACAA GGAATGTCTA ATCAATATTT CCAAATACAA GTTTTCTTTG
GTTATAAGCG GCCTCACTAC TATTTTAAAG AATGTTAACA ATATGAGAAT ATTTGGAGAA
GCTGCTGAAA AAAATTTATA TCTCTCTCAG TTGATTATAT TGGATACACT GGAAAAATGT
CTTGCTGGGC AACCAAAGGA CACAATGAGA TTAGATGAAA CGATGCTGGT CAAACAGTTG
CTGCCAGAAA TCTGCCATTT TCTTCACACC TGTCGTGAAG GAAACCAGCA TGCAGCTGAA
CTTCGGAATT CTGCCTCTGG GGTTTTATTT TCTCTCAGCT GCAACAACTT CAATGCAGTC
TTTAGTCGCA TTTCTACCAG GTTACAGGAA TTAACTGTTT GTTCAGAAGA CAATGTTGAT
GTTCATGATA TAGAATTGTT ACAGTATATC AATGTGGATT GTGCAAAATT AAAACGACTC
CTGAAGGAAA CAGCATTTAA ATTTAAAGCC CTAAAGAAGG TTGCGCAGTT AGCAGTTATA
AATAGCCTGG AAAAGGCATT TTGGAACTGG GTAGAAAATT ATCCAGATGA ATTTACAAAA
CTGTACCAGA TCCCACAGAC TGATATGGCT GAATGTGCAG AAAAGCTATT TGACTTGGTG
GATGGTTTTG CTGAAAGCAC CAAACGTAAA GCAGCAGTTT GGCCACTACA AATCATTCTC
CTTATCTTGT GTCCAGAAAT AATCCAGGAT ATATCCAAAG ACGTGGTTGA TGAAAACAAC
ATGAATAAGA AGTTATTTCT GGACAGTCTA CGAAAAGCTC TTGCTGGCCA TGGAGGAAGT
AGGCAGCTGA CAGAAAGTGC TGCAATTGCC TGTGTCAAAC TGTGTAAAGC AAGTACTTAC
ATCAATTGGG AAGATAACTC TGTCATTTTC CTACTTGTTC AGTCCATGGT GGTTGATCTT
AAGAACCTGC TTTTTAATCC AAGTAAGCCA TTCTCAAGAG GCAGTCAGCC TGCAGATGTG
GATCTAATGA TTGACTGCCT TGTTTCTTGC TTTCGTATAA GCCCTCACAA CAACCAACAC
TTTAAGATCT GCCTGGCTCA GAATTCACCT TCTACATTTC ACTATGTGCT GGTAAATTCA
CTCCATCGAA TCATCACCAA TAGCACTTTC AAGCATGGAC TTGGCACTGC TTCCGCATTG
GATTGGTGGC CTAAGATTGA TGCTGTGTAT TGTCACTCGG TTGAACTTCG AAATATGTTT
GGTGAAACAC TTCATAAAGC AGTGCAAGGT TGTGGAGCAC ACCCAGCAAT ACGAATGGCA
CCGAGTCTTA CATTTAAAGA AAAAGTAACA AGCCTTAAAT TTAAAGAAAA ACCTACAGAC
CTGGAGACAA GAAGCTATAA GTATCTTCTC TTGTCCATGG TGAAACTAAT TCATGCAGAT
CCAAAGCTCT TGCTTTGTAA TCCAAGAAAA CAGGGGCCCG AAACCCAAGG CAGTACAGCA
GAATTAATTA CAGGGCTCGT CCAACTGGTC CCTCAGTCAC ACATGCCAGA GATTGCTCAG
GAAGCAATGG AGGCTCTGCT GGTTCTTCAT CAGTTAGATA GCATTGATTT GTGGAATCCT
GATGCTCCTG TAGAAACATT TTGGGAGATT AGCTCACAAA TGCTTTTTTA CATCTGCAAG
AAATTAACTA GTCATCAAAT GCTTAGTAGC ACAGAAATTC TCAAGTGGTT GCGGGAAATA
TTGATCTGCA GGAATAAATT TCTTCTTAAA AATAAGCAGG CAGATAGAAG TTCCTGTCAC
TTTCTCCTTT TTTACGGGGT AGGATGTGAT ATTCCTTCTA GTGGAAATAC CAGTCAAATG
TCCATGGATC ATGAAGAATT ACTACGTACT CCTGGAGCCT CTCTCCGGAA GGGAAAAGGG
AACTCCTCTA TGGATAGTGC AGCAGGATGC AGCGGAACCC CCCCGATTTG CCGACAAGCC
CAGACCAAAC TAGAAGTGGC CCTGTACATG TTTCTGTGGA ACCCTGACAC TGAAGCTGTT
CTGGTTGCCA TGTCCTGTTT CCGCCACCTC TGTGAGGAAG CAGATATCCG GTGTGGGGTG
GATGAAGTGT CAGTGCATAA CCTCTTGCCC AACTATAACA CATTCATGGA GTTTGCCTCT
GTCAGCAATA TGATGTCAAC AGGAAGAGCA GCACTTCAGA AAAGAGTGAT GGCACTGCTG
AGGCGCATTG AGCATCCCAC TGCAGGAAAC ACTGAGGCTT GGGAAGATAC ACATGCAAAA
TGGGAACAAG CAACAAAGCT AATCCTTAAC TATCCAAAAG CCAAAATGGA AGATGGCCAG
GCTGCTGAAA GCCTTCACAA GACCATTGTT AAGAGGCGAA TGTCCCATGT GAGTGGAGGA
GGATCCATAG ATTTGTCTGA CACAGACTCC CTACAGGAAT GGATCAACAT GACTGGCTTC
CTTTGTGCCC TTGGGGGAGT GTGCCTCCAG CAGAGAAGCA ATTCTGGCCT GGCAACCTAT
AGCCCACCCA TGGGTCCAGT CAGTGAACGT AAGGGTTCTA TGATTTCAGT GATGTCTTCA
GAGGGAAACG CAGATACACC TGTCAGCAAA TTTATGGATC GGCTGTTGTC CTTAATGGTG
TGTAACCATG AGAAAGTGGG ACTTCAAATA CGGACCAATG TTAAGGATCT GGTGGGTCTA
GAATTGAGTC CTGCTCTGTA TCCAATGCTA TTTAACAAAT TGAAGAATAC CATCAGCAAG
TTTTTTGACT CCCAAGGACA GGTTTTATTG ACTGATACCA ATACTCAATT TGTAGAACAA
ACCATAGCTA TAATGAAGAA CTTGCTAGAT AATCATACTG AAGGCAGCTC TGAACATCTA
GGGCAAGCTA GCATTGAAAC AATGATGTTA AATCTGGTCA GGTATGTTCG TGTGCTTGGG
AATATGGTCC ATGCAATTCA AATAAAAACG AAACTGTGTC AATTAGTTGA AGTAATGATG
GCAAGGAGAG ATGACCTCTC ATTTTGCCAA GAGATGAAAT TTAGGAATAA GATGGTAGAA
TACCTGACAG ACTGGGTTAT GGGAACATCA AACCAAGCAG CAGATGATGA TGTAAAATGT
CTTACAAGAG ATTTGGACCA GGCAAGCATG GAAGCAGTAG TTTCACTTCT AGCTGGTCTC
CCTCTGCAGC CTGAAGAAGG AGATGGTGTG GAATTGATGG AAGCCAAATC ACAGTTATTT
CTTAAATACT TCACATTATT TATGAACCTT TTGAATGACT GCAGTGAAGT TGAAGATGAA
AGTGCGCAAA CAGGTGGCAG GAAACGTGGC ATGTCTCGGA GGCTGGCATC ACTGAGGCAC
TGTACGGTCC TTGCAATGTC AAACTTACTC AATGCCAACG TAGACAGTGG TCTCATGCAC
TCCATAGGCT TAGGTTACCA CAAGGATCTC CAGACAAGAG CTACATTTAT GGAAGTTCTG
ACAAAAATCC TTCAACAAGG CACAGAATTT GACACACTTG CAGAAACAGT ATTGGCTGAT
CGGTTTGAGA GATTGGTGGA ACTGGTCACA ATGATGGGTG ATCAAGGAGA ACTCCCTATA
GCGATGGCTC TGGCCAATGT GGTTCCTTGT TCTCAGTGGG ATGAACTAGC TCGAGTTCTG
GTTACTCTGT TTGATTCTCG GCATTTACTC TACCAACTGC TCTGGAACAT GTTTTCTAAA
GAAGTAGAAT TGGCAGACTC CATGCAGACT CTCTTCCGAG GCAACAGCTT GGCCAGTAAA
ATAATGACAT TCTGTTTCAA GGTATATGGT GCTACCTATC TACAAAAACT CCTGGATCCT
TTATTACGAA TTGTGATCAC ATCCTCTGAT TGGCAACATG TTAGCTTTGA AGTGGATCCT
ACCAGGTTAG AACCATCAGA GAGCCTTGAG GAAAACCAGC GGAACCTCCT TCAGATGACT
GAAAAGTTCT TCCATGCCAT CATCAGTTCC TCCTCAGAAT TCCCCCCTCA ACTTCGAAGT
GTGTGCCACT GTTTATACCA GGCAACTTGC CACTCCCTAC TGAATAAAGC TACAGTAAAA
GAAAAAAAGG AAAACAAAAA ATCAGTGGTT AGCCAGCGTT TCCCTCAGAA CAGCATCGGT
GCAGTAGGAA GTGCCATGTT CCTCAGATTT ATCAATCCTG CCATTGTCTC ACCGTATGAA
GCAGGGATTT TAGATAAAAA GCCACCACCT AGAATCGAAA GGGGCTTGAA GTTAATGTCA
AAGATACTTC AGAGTATTGC CAATCATGTT CTCTTCACAA AAGAAGAACA TATGCGGCCT
TTCAATGATT TTGTGAAAAG CAACTTTGAT GCAGCACGCA GGTTTTTCCT TGATATAGCA
TCTGATTGTC CTACAAGTGA TGCAGTAAAT CATAGTCTTT CCTTCATAAG TGACGGCAAT
GTGCTTGCTT TACATCGTCT ACTCTGGAAC AATCAGGAGA AAATTGGGCA GTATCTTTCC
AGCAACAGGG ATCATAAAGC TGTTGGAAGA CGACCTTTTG ATAAGATGGC AACACTTCTT
GCATACCTGG GTCCTCCAGA GCACAAACCT GTGGCAGATA CACACTGGTC CAGCCTTAAC
CTTACCAGTT CAAAGTTTGA GGAATTTATG ACTAGGCATC AGGTACATGA AAAAGAAGAA
TTCAAGGCTT TGAAAACGTT AAGTATTTTC TACCAAGCTG GGACTTCCAA AGCTGGGAAT
CCTATTTTTT ATTATGTTGC ACGGAGGTTC AAAACTGGTC AAATCAATGG TGATTTGCTG
ATATACCATG TCTTACTGAC TTTAAAGCCA TATTATGCAA AGCCATATGA AATTGTAGTG
GACCTTACCC ATACCGGGCC TAGCAATCGC TTTAAAACAG ACTTTCTCTC TAAGTGGTTT
GTTGTTTTTC CTGGCTTTGC TTACGACAAC GTCTCCGCAG TCTATATCTA TAACTGTAAC
TCCTGGGTCA GGGAGTACAC CAAGTATCAT GAGCGGCTGC TGACTGGCCT CAAAGGTAGC
AAAAGGCTTG TTTTCATAGA CTGTCCTGGG AAACTGGCTG AGCACATAGA GCATGAACAA
CAGAAACTAC CTGCTGCCAC CTTGGCTTTA GAAGAGGACC TGAAGGTATT CCACAATGCT
CTCAAGCTAG CTCACAAAGA CACCAAAGTT TCTATTAAAG TTGGTTCTAC TGCTGTCCAA
GTAACTTCAG CAGAGCGAAC AAAAGTCCTA GGGCAATCAG TCTTTCTAAA TGACATTTAT
TATGCTTCGG AAATTGAAGA AATCTGCCTA GTAGATGAGA ACCAGTTCAC CTTAACCATT
GCAAACCAGG GCACGCCGCT CACCTTCATG CACCAGGAGT GTGAAGCCAT TGTCCAGTCT
ATCATTCATA TCCGGACCCG CTGGGAACTG TCACAGCCCG ACTCTATCCC CCAACACACC
AAGATTCGGC CAAAAGATGT CCCTGGGACA CTGCTCAATA TCGCATTACT TAATTTAGGC
AGTTCTGACC CGAGTTTACG GTCAGCTGCC TATAATCTTC TGTGTGCCTT AACTTGTACC
TTTAATTTAA AAATCGAGGG CCAGTTACTA GAGACATCAG GTTTATGTAT CCCTGCCAAC
AACACCCTCT TTATTGTCTC TATTAGTAAG ACACTGGCAG CCAATGAGCC ACACCTCACG
TTAGAATTTT TGGAAGAGTG TATTTCTGGA TTTAGCAAAT CTAGTATTGA ATTGAAACAC
CTTTGTTTGG AATACATGAC TCCATGGCTG TCAAATCTAG TTCGTTTTTG CAAGCATAAT
GATGATGCCA AACGACAAAG AGTTACTGCT ATTCTTGACA AGCTGATAAC AATGACCATC
AATGAAAAAC AGATGTACCC ATCTATTCAA GCAAAAATAT GGGGAAGCCT TGGGCAGATT
ACAGATCTGC TTGATGTTGT ACTAGACAGT TTCATCAAAA CCAGTGCAAC AGGTGGCTTG
GGATCAATAA AAGCTGAGGT GATGGCAGAT ACTGCTGTAG CTTTGGCTTC TGGAAATGTG
AAATTGGTTT CAAGCAAGGT TATTGGAAGG ATGTGCAAAA TAATTGACAA GACATGCTTA
TCTCCAACTC CTACTTTAGA ACAACATCTT ATGTGGGATG ATATTGCTAT TTTAGCACGC
TACATGCTGA TGCTGTCCTT CAACAATTCC CTTGATGTGG CAGCTCATCT TCCCTACCTC
TTCCACGTTG TTACTTTCTT AGTAGCCACA GGTCCGCTCT CCCTTAGAGC TTCCACACAT
GGACTGGTCA TTAATATCAT TCACTCTCTG TGTACTTGTT CACAGCTTCA TTTTAGTGAA
GAGACCAAGC AAGTTTTGAG ACTCAGTCTG ACAGAGTTCT CATTACCCAA ATTTTACTTG
CTGTTTGGCA TTAGCAAAGT CAAGTCAGCT GCTGTCATTG CCTTCCGTTC CAGTTACCGG
GACAGGTCAT TCTCTCCTGG CTCCTATGAG AGAGAGACTT TTGCTTTGAC ATCCTTGGAA
ACAGTCACAG AAGCTTTGTT GGAGATCATG GAGGCATGCA TGAGAGATAT TCCAACGTGC
AAGTGGCTGG ACCAGTGGAC AGAACTAGCT CAAAGATTTG CATTCCAATA TAATCCATCC
CTGCAACCAA GAGCTCTTGT TGTCTTTGGG TGTATTAGCA AACGAGTGTC TCATGGGCAG
ATAAAGCAGA TAATCCGTAT TCTTAGCAAG GCACTTGAGA GTTGCTTAAA AGGACCTGAC
ACTTACAACA GTCAAGTTCT GATAGAAGCT ACAGTAATAG CACTAACCAA ATTACAGCCA
CTTCTTAATA AGGACTCGCC TCTGCACAAA GCCCTCTTTT GGGTAGCTGT GGCTGTGCTG
CAGCTTGATG AGGTCAACTT GTATTCAGCA GGTACCGCAC TTCTTGAACA AAACCTGCAT
ACTTTAGATA GTCTCCGTAT ATTCAATGAC AAGAGTCCAG AGGAAGTATT TATGGCAATC
CGGAATCCTC TGGAGTGGCA CTGCAAGCAA ATGGATCATT TTGTTGGACT CAATTTCAAC
TCTAACTTTA ACTTTGCATT GGTTGGACAC CTTTTAAAAG GGTACAGGCA TCCTTCACCT
GCTATTGTTG CAAGAACAGT CAGAATTTTA CATACACTAC TAACTCTGGT TAACAAACAC
AGAAATTGTG ACAAATTTGA AGTGAATACA CAGAGCGTGG CCTACTTAGC AGCTTTACTT
ACAGTGTCTG AAGAAGTTCG AAGTCGCTGC AGCCTAAAAC ATAGAAAGTC ACTTCTTCTT
ACTGATATTT CAATGGAAAA TGTTCCTATG GATACATATC CCATTCATCA TGGTGACCCT
TCCTATAGGA CACTAAAGGA GACTCAGCCA TGGTCCTCTC CCAAAGGTTC TGAAGGATAC
CTTGCAGCCA CCTATCCAAC TGTCGGCCAG ACCAGTCCCC GAGCCAGGAA ATCCATGAGC
CTGGACATGG GGCAACCTTC TCAGGCCAAC ACTAAGAAGT TGCTTGGAAC AAGGAAAAGT
TTTGATCACT TGATATCAGA CACAAAGGCT CCTAAAAGGC AAGAAATGGA ATCAGGGATC
ACAACACCCC CCAAAATGAG GAGAGTAGCA GAAACTGATT ATGAAATGGA AACTCAGAGG
ATTTCCTCAT CACAACAGCA CCCACATTTA CGTAAAGTTT CAGTGTCTGA ATCAAATGTT
CTCTTGGATG AAGAAGTACT TACTGATCCG AAGATCCAGG CGCTGCTTCT TACTGTTCTA
GCTACACTGG TAAAATATAC CACAGATGAG TTTGATCAAC GAATTCTTTA TGAATACTTA
GCAGAGGCCA GTGTTGTGTT TCCCAAAGTC TTTCCTGTTG TGCATAATTT GTTGGACTCT
AAGATCAACA CCCTGTTATC ATTGTGCCAA GATCCAAATT TGTTAAATCC AATCCATGGA
ATTGTGCAGA GTGTGGTGTA CCATGAAGAA TCCCCACCAC AATACCAAAC ATCTTACCTG
CAAAGTTTTG GTTTTAATGG CTTGTGGCGG TTTGCAGGAC CGTTTTCAAA GCAAACACAA
ATTCCAGACT ATGCTGAGCT TATTGTTAAG TTTCTTGATG CCTTGATTGA CACGTACCTG
CCTGGAATTG ATGAAGAAAC CAGTGAAGAA TCCCTCCTGA CTCCCACATC TCCTTACCCT
CCTGCACTGC AGAGCCAGCT TAGTATCACT GCCAACCTTA ACCTTTCTAA TTCCATGACC
TCACTTGCAA CTTCCCAGCA TTCCCCAGCT TCTCTGCCTT GCTCTAAATC AGCAGTTTTC
ATGCAGCTGT TCCCTCATCA AGGAATCGAC AAGGAGAACG TTGAACTCTC CCCTACCACT
GGCCACTGTA ACAGTGGACG AACTCGCCAC GGATCCGCAA GCCAAGTGCA GAAGCAAAGA
AGCGCTGGCA GTTTCAAACG TAATAGCATT AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523157.1
CDS898..9462
Misc Feature(1)4447..5559(+)
Misc Feature(2)4480..5541(+)
Misc Feature(3)4594..5262(+)
Misc Feature(4)5629..6072(+)
Misc Feature(5)5698..6003(+)
Misc Feature(6)5902..6000(+)
Misc Feature(7)6070..6399(+)
Misc Feature(8)6070..6399(+)
Translation

Target ORF information:

RefSeq Version XM_011524855
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524855

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
ATGTGGAATG CAAAGGAGAG GCTTCCAATA AAAACAGGAC AGCAGAACAC ACATACCAAA 
GTCAGTACTG AGCACAACAA GGAATGTCTA ATCAATATTT CCAAATACAA GTTTTCTTTG
GTTATAAGCG GCCTCACTAC TATTTTAAAG AATGTTAACA ATATGAGAAT ATTTGGAGAA
GCTGCTGAAA AAAATTTATA TCTCTCTCAG TTGATTATAT TGGATACACT GGAAAAATGT
CTTGCTGGGC AACCAAAGGA CACAATGAGA TTAGATGAAA CGATGCTGGT CAAACAGTTG
CTGCCAGAAA TCTGCCATTT TCTTCACACC TGTCGTGAAG GAAACCAGCA TGCAGCTGAA
CTTCGGAATT CTGCCTCTGG GGTTTTATTT TCTCTCAGCT GCAACAACTT CAATGCAGTC
TTTAGTCGCA TTTCTACCAG GTTACAGGAA TTAACTGTTT GTTCAGAAGA CAATGTTGAT
GTTCATGATA TAGAATTGTT ACAGTATATC AATGTGGATT GTGCAAAATT AAAACGACTC
CTGAAGGAAA CAGCATTTAA ATTTAAAGCC CTAAAGAAGG TTGCGCAGTT AGCAGTTATA
AATAGCCTGG AAAAGGCATT TTGGAACTGG GTAGAAAATT ATCCAGATGA ATTTACAAAA
CTGTACCAGA TCCCACAGAC TGATATGGCT GAATGTGCAG AAAAGCTATT TGACTTGGTG
GATGGTTTTG CTGAAAGCAC CAAACGTAAA GCAGCAGTTT GGCCACTACA AATCATTCTC
CTTATCTTGT GTCCAGAAAT AATCCAGGAT ATATCCAAAG ACGTGGTTGA TGAAAACAAC
ATGAATAAGA AGTTATTTCT GGACAGTCTA CGAAAAGCTC TTGCTGGCCA TGGAGGAAGT
AGGCAGCTGA CAGAAAGTGC TGCAATTGCC TGTGTCAAAC TGTGTAAAGC AAGTACTTAC
ATCAATTGGG AAGATAACTC TGTCATTTTC CTACTTGTTC AGTCCATGGT GGTTGATCTT
AAGAACCTGC TTTTTAATCC AAGTAAGCCA TTCTCAAGAG GCAGTCAGCC TGCAGATGTG
GATCTAATGA TTGACTGCCT TGTTTCTTGC TTTCGTATAA GCCCTCACAA CAACCAACAC
TTTAAGATCT GCCTGGCTCA GAATTCACCT TCTACATTTC ACTATGTGCT GGTAAATTCA
CTCCATCGAA TCATCACCAA TAGCACTTTC AAGCATGGAC TTGGCACTGC TTCCGCATTG
GATTGGTGGC CTAAGATTGA TGCTGTGTAT TGTCACTCGG TTGAACTTCG AAATATGTTT
GGTGAAACAC TTCATAAAGC AGTGCAAGGT TGTGGAGCAC ACCCAGCAAT ACGAATGGCA
CCGAGTCTTA CATTTAAAGA AAAAGTAACA AGCCTTAAAT TTAAAGAAAA ACCTACAGAC
CTGGAGACAA GAAGCTATAA GTATCTTCTC TTGTCCATGG TGAAACTAAT TCATGCAGAT
CCAAAGCTCT TGCTTTGTAA TCCAAGAAAA CAGGGGCCCG AAACCCAAGG CAGTACAGCA
GAATTAATTA CAGGGCTCGT CCAACTGGTC CCTCAGTCAC ACATGCCAGA GATTGCTCAG
GAAGCAATGG AGGCTCTGCT GGTTCTTCAT CAGTTAGATA GCATTGATTT GTGGAATCCT
GATGCTCCTG TAGAAACATT TTGGGAGATT AGCTCACAAA TGCTTTTTTA CATCTGCAAG
AAATTAACTA GTCATCAAAT GCTTAGTAGC ACAGAAATTC TCAAGTGGTT GCGGGAAATA
TTGATCTGCA GGAATAAATT TCTTCTTAAA AATAAGCAGG CAGATAGAAG TTCCTGTCAC
TTTCTCCTTT TTTACGGGGT AGGATGTGAT ATTCCTTCTA GTGGAAATAC CAGTCAAATG
TCCATGGATC ATGAAGAATT ACTACGTACT CCTGGAGCCT CTCTCCGGAA GGGAAAAGGG
AACTCCTCTA TGGATAGTGC AGCAGGATGC AGCGGAACCC CCCCGATTTG CCGACAAGCC
CAGACCAAAC TAGAAGTGGC CCTGTACATG TTTCTGTGGA ACCCTGACAC TGAAGCTGTT
CTGGTTGCCA TGTCCTGTTT CCGCCACCTC TGTGAGGAAG CAGATATCCG GTGTGGGGTG
GATGAAGTGT CAGTGCATAA CCTCTTGCCC AACTATAACA CATTCATGGA GTTTGCCTCT
GTCAGCAATA TGATGTCAAC AGGAAGAGCA GCACTTCAGA AAAGAGTGAT GGCACTGCTG
AGGCGCATTG AGCATCCCAC TGCAGGAAAC ACTGAGGCTT GGGAAGATAC ACATGCAAAA
TGGGAACAAG CAACAAAGCT AATCCTTAAC TATCCAAAAG CCAAAATGGA AGATGGCCAG
GCTGCTGAAA GCCTTCACAA GACCATTGTT AAGAGGCGAA TGTCCCATGT GAGTGGAGGA
GGATCCATAG ATTTGTCTGA CACAGACTCC CTACAGGAAT GGATCAACAT GACTGGCTTC
CTTTGTGCCC TTGGGGGAGT GTGCCTCCAG CAGAGAAGCA ATTCTGGCCT GGCAACCTAT
AGCCCACCCA TGGGTCCAGT CAGTGAACGT AAGGGTTCTA TGATTTCAGT GATGTCTTCA
GAGGGAAACG CAGATACACC TGTCAGCAAA TTTATGGATC GGCTGTTGTC CTTAATGGTG
TGTAACCATG AGAAAGTGGG ACTTCAAATA CGGACCAATG TTAAGGATCT GGTGGGTCTA
GAATTGAGTC CTGCTCTGTA TCCAATGCTA TTTAACAAAT TGAAGAATAC CATCAGCAAG
TTTTTTGACT CCCAAGGACA GGTTTTATTG ACTGATACCA ATACTCAATT TGTAGAACAA
ACCATAGCTA TAATGAAGAA CTTGCTAGAT AATCATACTG AAGGCAGCTC TGAACATCTA
GGGCAAGCTA GCATTGAAAC AATGATGTTA AATCTGGTCA GGTATGTTCG TGTGCTTGGG
AATATGGTCC ATGCAATTCA AATAAAAACG AAACTGTGTC AATTAGTTGA AGTAATGATG
GCAAGGAGAG ATGACCTCTC ATTTTGCCAA GAGATGAAAT TTAGGAATAA GATGGTAGAA
TACCTGACAG ACTGGGTTAT GGGAACATCA AACCAAGCAG CAGATGATGA TGTAAAATGT
CTTACAAGAG ATTTGGACCA GGCAAGCATG GAAGCAGTAG TTTCACTTCT AGCTGGTCTC
CCTCTGCAGC CTGAAGAAGG AGATGGTGTG GAATTGATGG AAGCCAAATC ACAGTTATTT
CTTAAATACT TCACATTATT TATGAACCTT TTGAATGACT GCAGTGAAGT TGAAGATGAA
AGTGCGCAAA CAGGTGGCAG GAAACGTGGC ATGTCTCGGA GGCTGGCATC ACTGAGGCAC
TGTACGGTCC TTGCAATGTC AAACTTACTC AATGCCAACG TAGACAGTGG TCTCATGCAC
TCCATAGGCT TAGGTTACCA CAAGGATCTC CAGACAAGAG CTACATTTAT GGAAGTTCTG
ACAAAAATCC TTCAACAAGG CACAGAATTT GACACACTTG CAGAAACAGT ATTGGCTGAT
CGGTTTGAGA GATTGGTGGA ACTGGTCACA ATGATGGGTG ATCAAGGAGA ACTCCCTATA
GCGATGGCTC TGGCCAATGT GGTTCCTTGT TCTCAGTGGG ATGAACTAGC TCGAGTTCTG
GTTACTCTGT TTGATTCTCG GCATTTACTC TACCAACTGC TCTGGAACAT GTTTTCTAAA
GAAGTAGAAT TGGCAGACTC CATGCAGACT CTCTTCCGAG GCAACAGCTT GGCCAGTAAA
ATAATGACAT TCTGTTTCAA GGTATATGGT GCTACCTATC TACAAAAACT CCTGGATCCT
TTATTACGAA TTGTGATCAC ATCCTCTGAT TGGCAACATG TTAGCTTTGA AGTGGATCCT
ACCAGGTTAG AACCATCAGA GAGCCTTGAG GAAAACCAGC GGAACCTCCT TCAGATGACT
GAAAAGTTCT TCCATGCCAT CATCAGTTCC TCCTCAGAAT TCCCCCCTCA ACTTCGAAGT
GTGTGCCACT GTTTATACCA GGCAACTTGC CACTCCCTAC TGAATAAAGC TACAGTAAAA
GAAAAAAAGG AAAACAAAAA ATCAGTGGTT AGCCAGCGTT TCCCTCAGAA CAGCATCGGT
GCAGTAGGAA GTGCCATGTT CCTCAGATTT ATCAATCCTG CCATTGTCTC ACCGTATGAA
GCAGGGATTT TAGATAAAAA GCCACCACCT AGAATCGAAA GGGGCTTGAA GTTAATGTCA
AAGATACTTC AGAGTATTGC CAATCATGTT CTCTTCACAA AAGAAGAACA TATGCGGCCT
TTCAATGATT TTGTGAAAAG CAACTTTGAT GCAGCACGCA GGTTTTTCCT TGATATAGCA
TCTGATTGTC CTACAAGTGA TGCAGTAAAT CATAGTCTTT CCTTCATAAG TGACGGCAAT
GTGCTTGCTT TACATCGTCT ACTCTGGAAC AATCAGGAGA AAATTGGGCA GTATCTTTCC
AGCAACAGGG ATCATAAAGC TGTTGGAAGA CGACCTTTTG ATAAGATGGC AACACTTCTT
GCATACCTGG GTCCTCCAGA GCACAAACCT GTGGCAGATA CACACTGGTC CAGCCTTAAC
CTTACCAGTT CAAAGTTTGA GGAATTTATG ACTAGGCATC AGGTACATGA AAAAGAAGAA
TTCAAGGCTT TGAAAACGTT AAGTATTTTC TACCAAGCTG GGACTTCCAA AGCTGGGAAT
CCTATTTTTT ATTATGTTGC ACGGAGGTTC AAAACTGGTC AAATCAATGG TGATTTGCTG
ATATACCATG TCTTACTGAC TTTAAAGCCA TATTATGCAA AGCCATATGA AATTGTAGTG
GACCTTACCC ATACCGGGCC TAGCAATCGC TTTAAAACAG ACTTTCTCTC TAAGTGGTTT
GTTGTTTTTC CTGGCTTTGC TTACGACAAC GTCTCCGCAG TCTATATCTA TAACTGTAAC
TCCTGGGTCA GGGAGTACAC CAAGTATCAT GAGCGGCTGC TGACTGGCCT CAAAGGTAGC
AAAAGGCTTG TTTTCATAGA CTGTCCTGGG AAACTGGCTG AGCACATAGA GCATGAACAA
CAGAAACTAC CTGCTGCCAC CTTGGCTTTA GAAGAGGACC TGAAGGTATT CCACAATGCT
CTCAAGCTAG CTCACAAAGA CACCAAAGTT TCTATTAAAG TTGGTTCTAC TGCTGTCCAA
GTAACTTCAG CAGAGCGAAC AAAAGTCCTA GGGCAATCAG TCTTTCTAAA TGACATTTAT
TATGCTTCGG AAATTGAAGA AATCTGCCTA GTAGATGAGA ACCAGTTCAC CTTAACCATT
GCAAACCAGG GCACGCCGCT CACCTTCATG CACCAGGAGT GTGAAGCCAT TGTCCAGTCT
ATCATTCATA TCCGGACCCG CTGGGAACTG TCACAGCCCG ACTCTATCCC CCAACACACC
AAGATTCGGC CAAAAGATGT CCCTGGGACA CTGCTCAATA TCGCATTACT TAATTTAGGC
AGTTCTGACC CGAGTTTACG GTCAGCTGCC TATAATCTTC TGTGTGCCTT AACTTGTACC
TTTAATTTAA AAATCGAGGG CCAGTTACTA GAGACATCAG GTTTATGTAT CCCTGCCAAC
AACACCCTCT TTATTGTCTC TATTAGTAAG ACACTGGCAG CCAATGAGCC ACACCTCACG
TTAGAATTTT TGGAAGAGTG TATTTCTGGA TTTAGCAAAT CTAGTATTGA ATTGAAACAC
CTTTGTTTGG AATACATGAC TCCATGGCTG TCAAATCTAG TTCGTTTTTG CAAGCATAAT
GATGATGCCA AACGACAAAG AGTTACTGCT ATTCTTGACA AGCTGATAAC AATGACCATC
AATGAAAAAC AGATGTACCC ATCTATTCAA GCAAAAATAT GGGGAAGCCT TGGGCAGATT
ACAGATCTGC TTGATGTTGT ACTAGACAGT TTCATCAAAA CCAGTGCAAC AGGTGGCTTG
GGATCAATAA AAGCTGAGGT GATGGCAGAT ACTGCTGTAG CTTTGGCTTC TGGAAATGTG
AAATTGGTTT CAAGCAAGGT TATTGGAAGG ATGTGCAAAA TAATTGACAA GACATGCTTA
TCTCCAACTC CTACTTTAGA ACAACATCTT ATGTGGGATG ATATTGCTAT TTTAGCACGC
TACATGCTGA TGCTGTCCTT CAACAATTCC CTTGATGTGG CAGCTCATCT TCCCTACCTC
TTCCACGTTG TTACTTTCTT AGTAGCCACA GGTCCGCTCT CCCTTAGAGC TTCCACACAT
GGACTGGTCA TTAATATCAT TCACTCTCTG TGTACTTGTT CACAGCTTCA TTTTAGTGAA
GAGACCAAGC AAGTTTTGAG ACTCAGTCTG ACAGAGTTCT CATTACCCAA ATTTTACTTG
CTGTTTGGCA TTAGCAAAGT CAAGTCAGCT GCTGTCATTG CCTTCCGTTC CAGTTACCGG
GACAGGTCAT TCTCTCCTGG CTCCTATGAG AGAGAGACTT TTGCTTTGAC ATCCTTGGAA
ACAGTCACAG AAGCTTTGTT GGAGATCATG GAGGCATGCA TGAGAGATAT TCCAACGTGC
AAGTGGCTGG ACCAGTGGAC AGAACTAGCT CAAAGATTTG CATTCCAATA TAATCCATCC
CTGCAACCAA GAGCTCTTGT TGTCTTTGGG TGTATTAGCA AACGAGTGTC TCATGGGCAG
ATAAAGCAGA TAATCCGTAT TCTTAGCAAG GCACTTGAGA GTTGCTTAAA AGGACCTGAC
ACTTACAACA GTCAAGTTCT GATAGAAGCT ACAGTAATAG CACTAACCAA ATTACAGCCA
CTTCTTAATA AGGACTCGCC TCTGCACAAA GCCCTCTTTT GGGTAGCTGT GGCTGTGCTG
CAGCTTGATG AGGTCAACTT GTATTCAGCA GGTACCGCAC TTCTTGAACA AAACCTGCAT
ACTTTAGATA GTCTCCGTAT ATTCAATGAC AAGAGTCCAG AGGAAGTATT TATGGCAATC
CGGAATCCTC TGGAGTGGCA CTGCAAGCAA ATGGATCATT TTGTTGGACT CAATTTCAAC
TCTAACTTTA ACTTTGCATT GGTTGGACAC CTTTTAAAAG GGTACAGGCA TCCTTCACCT
GCTATTGTTG CAAGAACAGT CAGAATTTTA CATACACTAC TAACTCTGGT TAACAAACAC
AGAAATTGTG ACAAATTTGA AGTGAATACA CAGAGCGTGG CCTACTTAGC AGCTTTACTT
ACAGTGTCTG AAGAAGTTCG AAGTCGCTGC AGCCTAAAAC ATAGAAAGTC ACTTCTTCTT
ACTGATATTT CAATGGAAAA TGTTCCTATG GATACATATC CCATTCATCA TGGTGACCCT
TCCTATAGGA CACTAAAGGA GACTCAGCCA TGGTCCTCTC CCAAAGGTTC TGAAGGATAC
CTTGCAGCCA CCTATCCAAC TGTCGGCCAG ACCAGTCCCC GAGCCAGGAA ATCCATGAGC
CTGGACATGG GGCAACCTTC TCAGGCCAAC ACTAAGAAGT TGCTTGGAAC AAGGAAAAGT
TTTGATCACT TGATATCAGA CACAAAGGCT CCTAAAAGGC AAGAAATGGA ATCAGGGATC
ACAACACCCC CCAAAATGAG GAGAGTAGCA GAAACTGATT ATGAAATGGA AACTCAGAGG
ATTTCCTCAT CACAACAGCA CCCACATTTA CGTAAAGTTT CAGTGTCTGA ATCAAATGTT
CTCTTGGATG AAGAAGTACT TACTGATCCG AAGATCCAGG CGCTGCTTCT TACTGTTCTA
GCTACACTGG TAAAATATAC CACAGATGAG TTTGATCAAC GAATTCTTTA TGAATACTTA
GCAGAGGCCA GTGTTGTGTT TCCCAAAGTC TTTCCTGTTG TGCATAATTT GTTGGACTCT
AAGATCAACA CCCTGTTATC ATTGTGCCAA GATCCAAATT TGTTAAATCC AATCCATGGA
ATTGTGCAGA GTGTGGTGTA CCATGAAGAA TCCCCACCAC AATACCAAAC ATCTTACCTG
CAAAGTTTTG GTTTTAATGG CTTGTGGCGG TTTGCAGGAC CGTTTTCAAA GCAAACACAA
ATTCCAGACT ATGCTGAGCT TATTGTTAAG TTTCTTGATG CCTTGATTGA CACGTACCTG
CCTGGAATTG ATGAAGAAAC CAGTGAAGAA TCCCTCCTGA CTCCCACATC TCCTTACCCT
CCTGCACTGC AGAGCCAGCT TAGTATCACT GCCAACCTTA ACCTTTCTAA TTCCATGACC
TCACTTGCAA CTTCCCAGCA TTCCCCAGCT TCTCTGCCTT GCTCTAAATC AGCAGTTTTC
ATGCAGCTGT TCCCTCATCA AGGAATCGAC AAGGAGAACG TTGAACTCTC CCCTACCACT
GGCCACTGTA ACAGTGGACG AACTCGCCAC GGATCCGCAA GCCAAGTGCA GAAGCAAAGA
AGCGCTGGCA GTTTCAAACG TAATAGCATT AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50429
Clone ID Related Accession (Same CDS sequence) XM_006721923 , XM_011524853 , XM_011524854 , XM_011524855 , XM_011524856
Accession Version XM_011524856.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8565bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neurofibromin isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
ATGTGGAATG CAAAGGAGAG GCTTCCAATA AAAACAGGAC AGCAGAACAC ACATACCAAA 
GTCAGTACTG AGCACAACAA GGAATGTCTA ATCAATATTT CCAAATACAA GTTTTCTTTG
GTTATAAGCG GCCTCACTAC TATTTTAAAG AATGTTAACA ATATGAGAAT ATTTGGAGAA
GCTGCTGAAA AAAATTTATA TCTCTCTCAG TTGATTATAT TGGATACACT GGAAAAATGT
CTTGCTGGGC AACCAAAGGA CACAATGAGA TTAGATGAAA CGATGCTGGT CAAACAGTTG
CTGCCAGAAA TCTGCCATTT TCTTCACACC TGTCGTGAAG GAAACCAGCA TGCAGCTGAA
CTTCGGAATT CTGCCTCTGG GGTTTTATTT TCTCTCAGCT GCAACAACTT CAATGCAGTC
TTTAGTCGCA TTTCTACCAG GTTACAGGAA TTAACTGTTT GTTCAGAAGA CAATGTTGAT
GTTCATGATA TAGAATTGTT ACAGTATATC AATGTGGATT GTGCAAAATT AAAACGACTC
CTGAAGGAAA CAGCATTTAA ATTTAAAGCC CTAAAGAAGG TTGCGCAGTT AGCAGTTATA
AATAGCCTGG AAAAGGCATT TTGGAACTGG GTAGAAAATT ATCCAGATGA ATTTACAAAA
CTGTACCAGA TCCCACAGAC TGATATGGCT GAATGTGCAG AAAAGCTATT TGACTTGGTG
GATGGTTTTG CTGAAAGCAC CAAACGTAAA GCAGCAGTTT GGCCACTACA AATCATTCTC
CTTATCTTGT GTCCAGAAAT AATCCAGGAT ATATCCAAAG ACGTGGTTGA TGAAAACAAC
ATGAATAAGA AGTTATTTCT GGACAGTCTA CGAAAAGCTC TTGCTGGCCA TGGAGGAAGT
AGGCAGCTGA CAGAAAGTGC TGCAATTGCC TGTGTCAAAC TGTGTAAAGC AAGTACTTAC
ATCAATTGGG AAGATAACTC TGTCATTTTC CTACTTGTTC AGTCCATGGT GGTTGATCTT
AAGAACCTGC TTTTTAATCC AAGTAAGCCA TTCTCAAGAG GCAGTCAGCC TGCAGATGTG
GATCTAATGA TTGACTGCCT TGTTTCTTGC TTTCGTATAA GCCCTCACAA CAACCAACAC
TTTAAGATCT GCCTGGCTCA GAATTCACCT TCTACATTTC ACTATGTGCT GGTAAATTCA
CTCCATCGAA TCATCACCAA TAGCACTTTC AAGCATGGAC TTGGCACTGC TTCCGCATTG
GATTGGTGGC CTAAGATTGA TGCTGTGTAT TGTCACTCGG TTGAACTTCG AAATATGTTT
GGTGAAACAC TTCATAAAGC AGTGCAAGGT TGTGGAGCAC ACCCAGCAAT ACGAATGGCA
CCGAGTCTTA CATTTAAAGA AAAAGTAACA AGCCTTAAAT TTAAAGAAAA ACCTACAGAC
CTGGAGACAA GAAGCTATAA GTATCTTCTC TTGTCCATGG TGAAACTAAT TCATGCAGAT
CCAAAGCTCT TGCTTTGTAA TCCAAGAAAA CAGGGGCCCG AAACCCAAGG CAGTACAGCA
GAATTAATTA CAGGGCTCGT CCAACTGGTC CCTCAGTCAC ACATGCCAGA GATTGCTCAG
GAAGCAATGG AGGCTCTGCT GGTTCTTCAT CAGTTAGATA GCATTGATTT GTGGAATCCT
GATGCTCCTG TAGAAACATT TTGGGAGATT AGCTCACAAA TGCTTTTTTA CATCTGCAAG
AAATTAACTA GTCATCAAAT GCTTAGTAGC ACAGAAATTC TCAAGTGGTT GCGGGAAATA
TTGATCTGCA GGAATAAATT TCTTCTTAAA AATAAGCAGG CAGATAGAAG TTCCTGTCAC
TTTCTCCTTT TTTACGGGGT AGGATGTGAT ATTCCTTCTA GTGGAAATAC CAGTCAAATG
TCCATGGATC ATGAAGAATT ACTACGTACT CCTGGAGCCT CTCTCCGGAA GGGAAAAGGG
AACTCCTCTA TGGATAGTGC AGCAGGATGC AGCGGAACCC CCCCGATTTG CCGACAAGCC
CAGACCAAAC TAGAAGTGGC CCTGTACATG TTTCTGTGGA ACCCTGACAC TGAAGCTGTT
CTGGTTGCCA TGTCCTGTTT CCGCCACCTC TGTGAGGAAG CAGATATCCG GTGTGGGGTG
GATGAAGTGT CAGTGCATAA CCTCTTGCCC AACTATAACA CATTCATGGA GTTTGCCTCT
GTCAGCAATA TGATGTCAAC AGGAAGAGCA GCACTTCAGA AAAGAGTGAT GGCACTGCTG
AGGCGCATTG AGCATCCCAC TGCAGGAAAC ACTGAGGCTT GGGAAGATAC ACATGCAAAA
TGGGAACAAG CAACAAAGCT AATCCTTAAC TATCCAAAAG CCAAAATGGA AGATGGCCAG
GCTGCTGAAA GCCTTCACAA GACCATTGTT AAGAGGCGAA TGTCCCATGT GAGTGGAGGA
GGATCCATAG ATTTGTCTGA CACAGACTCC CTACAGGAAT GGATCAACAT GACTGGCTTC
CTTTGTGCCC TTGGGGGAGT GTGCCTCCAG CAGAGAAGCA ATTCTGGCCT GGCAACCTAT
AGCCCACCCA TGGGTCCAGT CAGTGAACGT AAGGGTTCTA TGATTTCAGT GATGTCTTCA
GAGGGAAACG CAGATACACC TGTCAGCAAA TTTATGGATC GGCTGTTGTC CTTAATGGTG
TGTAACCATG AGAAAGTGGG ACTTCAAATA CGGACCAATG TTAAGGATCT GGTGGGTCTA
GAATTGAGTC CTGCTCTGTA TCCAATGCTA TTTAACAAAT TGAAGAATAC CATCAGCAAG
TTTTTTGACT CCCAAGGACA GGTTTTATTG ACTGATACCA ATACTCAATT TGTAGAACAA
ACCATAGCTA TAATGAAGAA CTTGCTAGAT AATCATACTG AAGGCAGCTC TGAACATCTA
GGGCAAGCTA GCATTGAAAC AATGATGTTA AATCTGGTCA GGTATGTTCG TGTGCTTGGG
AATATGGTCC ATGCAATTCA AATAAAAACG AAACTGTGTC AATTAGTTGA AGTAATGATG
GCAAGGAGAG ATGACCTCTC ATTTTGCCAA GAGATGAAAT TTAGGAATAA GATGGTAGAA
TACCTGACAG ACTGGGTTAT GGGAACATCA AACCAAGCAG CAGATGATGA TGTAAAATGT
CTTACAAGAG ATTTGGACCA GGCAAGCATG GAAGCAGTAG TTTCACTTCT AGCTGGTCTC
CCTCTGCAGC CTGAAGAAGG AGATGGTGTG GAATTGATGG AAGCCAAATC ACAGTTATTT
CTTAAATACT TCACATTATT TATGAACCTT TTGAATGACT GCAGTGAAGT TGAAGATGAA
AGTGCGCAAA CAGGTGGCAG GAAACGTGGC ATGTCTCGGA GGCTGGCATC ACTGAGGCAC
TGTACGGTCC TTGCAATGTC AAACTTACTC AATGCCAACG TAGACAGTGG TCTCATGCAC
TCCATAGGCT TAGGTTACCA CAAGGATCTC CAGACAAGAG CTACATTTAT GGAAGTTCTG
ACAAAAATCC TTCAACAAGG CACAGAATTT GACACACTTG CAGAAACAGT ATTGGCTGAT
CGGTTTGAGA GATTGGTGGA ACTGGTCACA ATGATGGGTG ATCAAGGAGA ACTCCCTATA
GCGATGGCTC TGGCCAATGT GGTTCCTTGT TCTCAGTGGG ATGAACTAGC TCGAGTTCTG
GTTACTCTGT TTGATTCTCG GCATTTACTC TACCAACTGC TCTGGAACAT GTTTTCTAAA
GAAGTAGAAT TGGCAGACTC CATGCAGACT CTCTTCCGAG GCAACAGCTT GGCCAGTAAA
ATAATGACAT TCTGTTTCAA GGTATATGGT GCTACCTATC TACAAAAACT CCTGGATCCT
TTATTACGAA TTGTGATCAC ATCCTCTGAT TGGCAACATG TTAGCTTTGA AGTGGATCCT
ACCAGGTTAG AACCATCAGA GAGCCTTGAG GAAAACCAGC GGAACCTCCT TCAGATGACT
GAAAAGTTCT TCCATGCCAT CATCAGTTCC TCCTCAGAAT TCCCCCCTCA ACTTCGAAGT
GTGTGCCACT GTTTATACCA GGCAACTTGC CACTCCCTAC TGAATAAAGC TACAGTAAAA
GAAAAAAAGG AAAACAAAAA ATCAGTGGTT AGCCAGCGTT TCCCTCAGAA CAGCATCGGT
GCAGTAGGAA GTGCCATGTT CCTCAGATTT ATCAATCCTG CCATTGTCTC ACCGTATGAA
GCAGGGATTT TAGATAAAAA GCCACCACCT AGAATCGAAA GGGGCTTGAA GTTAATGTCA
AAGATACTTC AGAGTATTGC CAATCATGTT CTCTTCACAA AAGAAGAACA TATGCGGCCT
TTCAATGATT TTGTGAAAAG CAACTTTGAT GCAGCACGCA GGTTTTTCCT TGATATAGCA
TCTGATTGTC CTACAAGTGA TGCAGTAAAT CATAGTCTTT CCTTCATAAG TGACGGCAAT
GTGCTTGCTT TACATCGTCT ACTCTGGAAC AATCAGGAGA AAATTGGGCA GTATCTTTCC
AGCAACAGGG ATCATAAAGC TGTTGGAAGA CGACCTTTTG ATAAGATGGC AACACTTCTT
GCATACCTGG GTCCTCCAGA GCACAAACCT GTGGCAGATA CACACTGGTC CAGCCTTAAC
CTTACCAGTT CAAAGTTTGA GGAATTTATG ACTAGGCATC AGGTACATGA AAAAGAAGAA
TTCAAGGCTT TGAAAACGTT AAGTATTTTC TACCAAGCTG GGACTTCCAA AGCTGGGAAT
CCTATTTTTT ATTATGTTGC ACGGAGGTTC AAAACTGGTC AAATCAATGG TGATTTGCTG
ATATACCATG TCTTACTGAC TTTAAAGCCA TATTATGCAA AGCCATATGA AATTGTAGTG
GACCTTACCC ATACCGGGCC TAGCAATCGC TTTAAAACAG ACTTTCTCTC TAAGTGGTTT
GTTGTTTTTC CTGGCTTTGC TTACGACAAC GTCTCCGCAG TCTATATCTA TAACTGTAAC
TCCTGGGTCA GGGAGTACAC CAAGTATCAT GAGCGGCTGC TGACTGGCCT CAAAGGTAGC
AAAAGGCTTG TTTTCATAGA CTGTCCTGGG AAACTGGCTG AGCACATAGA GCATGAACAA
CAGAAACTAC CTGCTGCCAC CTTGGCTTTA GAAGAGGACC TGAAGGTATT CCACAATGCT
CTCAAGCTAG CTCACAAAGA CACCAAAGTT TCTATTAAAG TTGGTTCTAC TGCTGTCCAA
GTAACTTCAG CAGAGCGAAC AAAAGTCCTA GGGCAATCAG TCTTTCTAAA TGACATTTAT
TATGCTTCGG AAATTGAAGA AATCTGCCTA GTAGATGAGA ACCAGTTCAC CTTAACCATT
GCAAACCAGG GCACGCCGCT CACCTTCATG CACCAGGAGT GTGAAGCCAT TGTCCAGTCT
ATCATTCATA TCCGGACCCG CTGGGAACTG TCACAGCCCG ACTCTATCCC CCAACACACC
AAGATTCGGC CAAAAGATGT CCCTGGGACA CTGCTCAATA TCGCATTACT TAATTTAGGC
AGTTCTGACC CGAGTTTACG GTCAGCTGCC TATAATCTTC TGTGTGCCTT AACTTGTACC
TTTAATTTAA AAATCGAGGG CCAGTTACTA GAGACATCAG GTTTATGTAT CCCTGCCAAC
AACACCCTCT TTATTGTCTC TATTAGTAAG ACACTGGCAG CCAATGAGCC ACACCTCACG
TTAGAATTTT TGGAAGAGTG TATTTCTGGA TTTAGCAAAT CTAGTATTGA ATTGAAACAC
CTTTGTTTGG AATACATGAC TCCATGGCTG TCAAATCTAG TTCGTTTTTG CAAGCATAAT
GATGATGCCA AACGACAAAG AGTTACTGCT ATTCTTGACA AGCTGATAAC AATGACCATC
AATGAAAAAC AGATGTACCC ATCTATTCAA GCAAAAATAT GGGGAAGCCT TGGGCAGATT
ACAGATCTGC TTGATGTTGT ACTAGACAGT TTCATCAAAA CCAGTGCAAC AGGTGGCTTG
GGATCAATAA AAGCTGAGGT GATGGCAGAT ACTGCTGTAG CTTTGGCTTC TGGAAATGTG
AAATTGGTTT CAAGCAAGGT TATTGGAAGG ATGTGCAAAA TAATTGACAA GACATGCTTA
TCTCCAACTC CTACTTTAGA ACAACATCTT ATGTGGGATG ATATTGCTAT TTTAGCACGC
TACATGCTGA TGCTGTCCTT CAACAATTCC CTTGATGTGG CAGCTCATCT TCCCTACCTC
TTCCACGTTG TTACTTTCTT AGTAGCCACA GGTCCGCTCT CCCTTAGAGC TTCCACACAT
GGACTGGTCA TTAATATCAT TCACTCTCTG TGTACTTGTT CACAGCTTCA TTTTAGTGAA
GAGACCAAGC AAGTTTTGAG ACTCAGTCTG ACAGAGTTCT CATTACCCAA ATTTTACTTG
CTGTTTGGCA TTAGCAAAGT CAAGTCAGCT GCTGTCATTG CCTTCCGTTC CAGTTACCGG
GACAGGTCAT TCTCTCCTGG CTCCTATGAG AGAGAGACTT TTGCTTTGAC ATCCTTGGAA
ACAGTCACAG AAGCTTTGTT GGAGATCATG GAGGCATGCA TGAGAGATAT TCCAACGTGC
AAGTGGCTGG ACCAGTGGAC AGAACTAGCT CAAAGATTTG CATTCCAATA TAATCCATCC
CTGCAACCAA GAGCTCTTGT TGTCTTTGGG TGTATTAGCA AACGAGTGTC TCATGGGCAG
ATAAAGCAGA TAATCCGTAT TCTTAGCAAG GCACTTGAGA GTTGCTTAAA AGGACCTGAC
ACTTACAACA GTCAAGTTCT GATAGAAGCT ACAGTAATAG CACTAACCAA ATTACAGCCA
CTTCTTAATA AGGACTCGCC TCTGCACAAA GCCCTCTTTT GGGTAGCTGT GGCTGTGCTG
CAGCTTGATG AGGTCAACTT GTATTCAGCA GGTACCGCAC TTCTTGAACA AAACCTGCAT
ACTTTAGATA GTCTCCGTAT ATTCAATGAC AAGAGTCCAG AGGAAGTATT TATGGCAATC
CGGAATCCTC TGGAGTGGCA CTGCAAGCAA ATGGATCATT TTGTTGGACT CAATTTCAAC
TCTAACTTTA ACTTTGCATT GGTTGGACAC CTTTTAAAAG GGTACAGGCA TCCTTCACCT
GCTATTGTTG CAAGAACAGT CAGAATTTTA CATACACTAC TAACTCTGGT TAACAAACAC
AGAAATTGTG ACAAATTTGA AGTGAATACA CAGAGCGTGG CCTACTTAGC AGCTTTACTT
ACAGTGTCTG AAGAAGTTCG AAGTCGCTGC AGCCTAAAAC ATAGAAAGTC ACTTCTTCTT
ACTGATATTT CAATGGAAAA TGTTCCTATG GATACATATC CCATTCATCA TGGTGACCCT
TCCTATAGGA CACTAAAGGA GACTCAGCCA TGGTCCTCTC CCAAAGGTTC TGAAGGATAC
CTTGCAGCCA CCTATCCAAC TGTCGGCCAG ACCAGTCCCC GAGCCAGGAA ATCCATGAGC
CTGGACATGG GGCAACCTTC TCAGGCCAAC ACTAAGAAGT TGCTTGGAAC AAGGAAAAGT
TTTGATCACT TGATATCAGA CACAAAGGCT CCTAAAAGGC AAGAAATGGA ATCAGGGATC
ACAACACCCC CCAAAATGAG GAGAGTAGCA GAAACTGATT ATGAAATGGA AACTCAGAGG
ATTTCCTCAT CACAACAGCA CCCACATTTA CGTAAAGTTT CAGTGTCTGA ATCAAATGTT
CTCTTGGATG AAGAAGTACT TACTGATCCG AAGATCCAGG CGCTGCTTCT TACTGTTCTA
GCTACACTGG TAAAATATAC CACAGATGAG TTTGATCAAC GAATTCTTTA TGAATACTTA
GCAGAGGCCA GTGTTGTGTT TCCCAAAGTC TTTCCTGTTG TGCATAATTT GTTGGACTCT
AAGATCAACA CCCTGTTATC ATTGTGCCAA GATCCAAATT TGTTAAATCC AATCCATGGA
ATTGTGCAGA GTGTGGTGTA CCATGAAGAA TCCCCACCAC AATACCAAAC ATCTTACCTG
CAAAGTTTTG GTTTTAATGG CTTGTGGCGG TTTGCAGGAC CGTTTTCAAA GCAAACACAA
ATTCCAGACT ATGCTGAGCT TATTGTTAAG TTTCTTGATG CCTTGATTGA CACGTACCTG
CCTGGAATTG ATGAAGAAAC CAGTGAAGAA TCCCTCCTGA CTCCCACATC TCCTTACCCT
CCTGCACTGC AGAGCCAGCT TAGTATCACT GCCAACCTTA ACCTTTCTAA TTCCATGACC
TCACTTGCAA CTTCCCAGCA TTCCCCAGCT TCTCTGCCTT GCTCTAAATC AGCAGTTTTC
ATGCAGCTGT TCCCTCATCA AGGAATCGAC AAGGAGAACG TTGAACTCTC CCCTACCACT
GGCCACTGTA ACAGTGGACG AACTCGCCAC GGATCCGCAA GCCAAGTGCA GAAGCAAAGA
AGCGCTGGCA GTTTCAAACG TAATAGCATT AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523158.1
CDS1576..10140
Misc Feature(1)5125..6237(+)
Misc Feature(2)5158..6219(+)
Misc Feature(3)5272..5940(+)
Misc Feature(4)6307..6750(+)
Misc Feature(5)6376..6681(+)
Misc Feature(6)6580..6678(+)
Misc Feature(7)6748..7077(+)
Misc Feature(8)6748..7077(+)
Translation

Target ORF information:

RefSeq Version XM_011524856
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524856

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
ATGTGGAATG CAAAGGAGAG GCTTCCAATA AAAACAGGAC AGCAGAACAC ACATACCAAA 
GTCAGTACTG AGCACAACAA GGAATGTCTA ATCAATATTT CCAAATACAA GTTTTCTTTG
GTTATAAGCG GCCTCACTAC TATTTTAAAG AATGTTAACA ATATGAGAAT ATTTGGAGAA
GCTGCTGAAA AAAATTTATA TCTCTCTCAG TTGATTATAT TGGATACACT GGAAAAATGT
CTTGCTGGGC AACCAAAGGA CACAATGAGA TTAGATGAAA CGATGCTGGT CAAACAGTTG
CTGCCAGAAA TCTGCCATTT TCTTCACACC TGTCGTGAAG GAAACCAGCA TGCAGCTGAA
CTTCGGAATT CTGCCTCTGG GGTTTTATTT TCTCTCAGCT GCAACAACTT CAATGCAGTC
TTTAGTCGCA TTTCTACCAG GTTACAGGAA TTAACTGTTT GTTCAGAAGA CAATGTTGAT
GTTCATGATA TAGAATTGTT ACAGTATATC AATGTGGATT GTGCAAAATT AAAACGACTC
CTGAAGGAAA CAGCATTTAA ATTTAAAGCC CTAAAGAAGG TTGCGCAGTT AGCAGTTATA
AATAGCCTGG AAAAGGCATT TTGGAACTGG GTAGAAAATT ATCCAGATGA ATTTACAAAA
CTGTACCAGA TCCCACAGAC TGATATGGCT GAATGTGCAG AAAAGCTATT TGACTTGGTG
GATGGTTTTG CTGAAAGCAC CAAACGTAAA GCAGCAGTTT GGCCACTACA AATCATTCTC
CTTATCTTGT GTCCAGAAAT AATCCAGGAT ATATCCAAAG ACGTGGTTGA TGAAAACAAC
ATGAATAAGA AGTTATTTCT GGACAGTCTA CGAAAAGCTC TTGCTGGCCA TGGAGGAAGT
AGGCAGCTGA CAGAAAGTGC TGCAATTGCC TGTGTCAAAC TGTGTAAAGC AAGTACTTAC
ATCAATTGGG AAGATAACTC TGTCATTTTC CTACTTGTTC AGTCCATGGT GGTTGATCTT
AAGAACCTGC TTTTTAATCC AAGTAAGCCA TTCTCAAGAG GCAGTCAGCC TGCAGATGTG
GATCTAATGA TTGACTGCCT TGTTTCTTGC TTTCGTATAA GCCCTCACAA CAACCAACAC
TTTAAGATCT GCCTGGCTCA GAATTCACCT TCTACATTTC ACTATGTGCT GGTAAATTCA
CTCCATCGAA TCATCACCAA TAGCACTTTC AAGCATGGAC TTGGCACTGC TTCCGCATTG
GATTGGTGGC CTAAGATTGA TGCTGTGTAT TGTCACTCGG TTGAACTTCG AAATATGTTT
GGTGAAACAC TTCATAAAGC AGTGCAAGGT TGTGGAGCAC ACCCAGCAAT ACGAATGGCA
CCGAGTCTTA CATTTAAAGA AAAAGTAACA AGCCTTAAAT TTAAAGAAAA ACCTACAGAC
CTGGAGACAA GAAGCTATAA GTATCTTCTC TTGTCCATGG TGAAACTAAT TCATGCAGAT
CCAAAGCTCT TGCTTTGTAA TCCAAGAAAA CAGGGGCCCG AAACCCAAGG CAGTACAGCA
GAATTAATTA CAGGGCTCGT CCAACTGGTC CCTCAGTCAC ACATGCCAGA GATTGCTCAG
GAAGCAATGG AGGCTCTGCT GGTTCTTCAT CAGTTAGATA GCATTGATTT GTGGAATCCT
GATGCTCCTG TAGAAACATT TTGGGAGATT AGCTCACAAA TGCTTTTTTA CATCTGCAAG
AAATTAACTA GTCATCAAAT GCTTAGTAGC ACAGAAATTC TCAAGTGGTT GCGGGAAATA
TTGATCTGCA GGAATAAATT TCTTCTTAAA AATAAGCAGG CAGATAGAAG TTCCTGTCAC
TTTCTCCTTT TTTACGGGGT AGGATGTGAT ATTCCTTCTA GTGGAAATAC CAGTCAAATG
TCCATGGATC ATGAAGAATT ACTACGTACT CCTGGAGCCT CTCTCCGGAA GGGAAAAGGG
AACTCCTCTA TGGATAGTGC AGCAGGATGC AGCGGAACCC CCCCGATTTG CCGACAAGCC
CAGACCAAAC TAGAAGTGGC CCTGTACATG TTTCTGTGGA ACCCTGACAC TGAAGCTGTT
CTGGTTGCCA TGTCCTGTTT CCGCCACCTC TGTGAGGAAG CAGATATCCG GTGTGGGGTG
GATGAAGTGT CAGTGCATAA CCTCTTGCCC AACTATAACA CATTCATGGA GTTTGCCTCT
GTCAGCAATA TGATGTCAAC AGGAAGAGCA GCACTTCAGA AAAGAGTGAT GGCACTGCTG
AGGCGCATTG AGCATCCCAC TGCAGGAAAC ACTGAGGCTT GGGAAGATAC ACATGCAAAA
TGGGAACAAG CAACAAAGCT AATCCTTAAC TATCCAAAAG CCAAAATGGA AGATGGCCAG
GCTGCTGAAA GCCTTCACAA GACCATTGTT AAGAGGCGAA TGTCCCATGT GAGTGGAGGA
GGATCCATAG ATTTGTCTGA CACAGACTCC CTACAGGAAT GGATCAACAT GACTGGCTTC
CTTTGTGCCC TTGGGGGAGT GTGCCTCCAG CAGAGAAGCA ATTCTGGCCT GGCAACCTAT
AGCCCACCCA TGGGTCCAGT CAGTGAACGT AAGGGTTCTA TGATTTCAGT GATGTCTTCA
GAGGGAAACG CAGATACACC TGTCAGCAAA TTTATGGATC GGCTGTTGTC CTTAATGGTG
TGTAACCATG AGAAAGTGGG ACTTCAAATA CGGACCAATG TTAAGGATCT GGTGGGTCTA
GAATTGAGTC CTGCTCTGTA TCCAATGCTA TTTAACAAAT TGAAGAATAC CATCAGCAAG
TTTTTTGACT CCCAAGGACA GGTTTTATTG ACTGATACCA ATACTCAATT TGTAGAACAA
ACCATAGCTA TAATGAAGAA CTTGCTAGAT AATCATACTG AAGGCAGCTC TGAACATCTA
GGGCAAGCTA GCATTGAAAC AATGATGTTA AATCTGGTCA GGTATGTTCG TGTGCTTGGG
AATATGGTCC ATGCAATTCA AATAAAAACG AAACTGTGTC AATTAGTTGA AGTAATGATG
GCAAGGAGAG ATGACCTCTC ATTTTGCCAA GAGATGAAAT TTAGGAATAA GATGGTAGAA
TACCTGACAG ACTGGGTTAT GGGAACATCA AACCAAGCAG CAGATGATGA TGTAAAATGT
CTTACAAGAG ATTTGGACCA GGCAAGCATG GAAGCAGTAG TTTCACTTCT AGCTGGTCTC
CCTCTGCAGC CTGAAGAAGG AGATGGTGTG GAATTGATGG AAGCCAAATC ACAGTTATTT
CTTAAATACT TCACATTATT TATGAACCTT TTGAATGACT GCAGTGAAGT TGAAGATGAA
AGTGCGCAAA CAGGTGGCAG GAAACGTGGC ATGTCTCGGA GGCTGGCATC ACTGAGGCAC
TGTACGGTCC TTGCAATGTC AAACTTACTC AATGCCAACG TAGACAGTGG TCTCATGCAC
TCCATAGGCT TAGGTTACCA CAAGGATCTC CAGACAAGAG CTACATTTAT GGAAGTTCTG
ACAAAAATCC TTCAACAAGG CACAGAATTT GACACACTTG CAGAAACAGT ATTGGCTGAT
CGGTTTGAGA GATTGGTGGA ACTGGTCACA ATGATGGGTG ATCAAGGAGA ACTCCCTATA
GCGATGGCTC TGGCCAATGT GGTTCCTTGT TCTCAGTGGG ATGAACTAGC TCGAGTTCTG
GTTACTCTGT TTGATTCTCG GCATTTACTC TACCAACTGC TCTGGAACAT GTTTTCTAAA
GAAGTAGAAT TGGCAGACTC CATGCAGACT CTCTTCCGAG GCAACAGCTT GGCCAGTAAA
ATAATGACAT TCTGTTTCAA GGTATATGGT GCTACCTATC TACAAAAACT CCTGGATCCT
TTATTACGAA TTGTGATCAC ATCCTCTGAT TGGCAACATG TTAGCTTTGA AGTGGATCCT
ACCAGGTTAG AACCATCAGA GAGCCTTGAG GAAAACCAGC GGAACCTCCT TCAGATGACT
GAAAAGTTCT TCCATGCCAT CATCAGTTCC TCCTCAGAAT TCCCCCCTCA ACTTCGAAGT
GTGTGCCACT GTTTATACCA GGCAACTTGC CACTCCCTAC TGAATAAAGC TACAGTAAAA
GAAAAAAAGG AAAACAAAAA ATCAGTGGTT AGCCAGCGTT TCCCTCAGAA CAGCATCGGT
GCAGTAGGAA GTGCCATGTT CCTCAGATTT ATCAATCCTG CCATTGTCTC ACCGTATGAA
GCAGGGATTT TAGATAAAAA GCCACCACCT AGAATCGAAA GGGGCTTGAA GTTAATGTCA
AAGATACTTC AGAGTATTGC CAATCATGTT CTCTTCACAA AAGAAGAACA TATGCGGCCT
TTCAATGATT TTGTGAAAAG CAACTTTGAT GCAGCACGCA GGTTTTTCCT TGATATAGCA
TCTGATTGTC CTACAAGTGA TGCAGTAAAT CATAGTCTTT CCTTCATAAG TGACGGCAAT
GTGCTTGCTT TACATCGTCT ACTCTGGAAC AATCAGGAGA AAATTGGGCA GTATCTTTCC
AGCAACAGGG ATCATAAAGC TGTTGGAAGA CGACCTTTTG ATAAGATGGC AACACTTCTT
GCATACCTGG GTCCTCCAGA GCACAAACCT GTGGCAGATA CACACTGGTC CAGCCTTAAC
CTTACCAGTT CAAAGTTTGA GGAATTTATG ACTAGGCATC AGGTACATGA AAAAGAAGAA
TTCAAGGCTT TGAAAACGTT AAGTATTTTC TACCAAGCTG GGACTTCCAA AGCTGGGAAT
CCTATTTTTT ATTATGTTGC ACGGAGGTTC AAAACTGGTC AAATCAATGG TGATTTGCTG
ATATACCATG TCTTACTGAC TTTAAAGCCA TATTATGCAA AGCCATATGA AATTGTAGTG
GACCTTACCC ATACCGGGCC TAGCAATCGC TTTAAAACAG ACTTTCTCTC TAAGTGGTTT
GTTGTTTTTC CTGGCTTTGC TTACGACAAC GTCTCCGCAG TCTATATCTA TAACTGTAAC
TCCTGGGTCA GGGAGTACAC CAAGTATCAT GAGCGGCTGC TGACTGGCCT CAAAGGTAGC
AAAAGGCTTG TTTTCATAGA CTGTCCTGGG AAACTGGCTG AGCACATAGA GCATGAACAA
CAGAAACTAC CTGCTGCCAC CTTGGCTTTA GAAGAGGACC TGAAGGTATT CCACAATGCT
CTCAAGCTAG CTCACAAAGA CACCAAAGTT TCTATTAAAG TTGGTTCTAC TGCTGTCCAA
GTAACTTCAG CAGAGCGAAC AAAAGTCCTA GGGCAATCAG TCTTTCTAAA TGACATTTAT
TATGCTTCGG AAATTGAAGA AATCTGCCTA GTAGATGAGA ACCAGTTCAC CTTAACCATT
GCAAACCAGG GCACGCCGCT CACCTTCATG CACCAGGAGT GTGAAGCCAT TGTCCAGTCT
ATCATTCATA TCCGGACCCG CTGGGAACTG TCACAGCCCG ACTCTATCCC CCAACACACC
AAGATTCGGC CAAAAGATGT CCCTGGGACA CTGCTCAATA TCGCATTACT TAATTTAGGC
AGTTCTGACC CGAGTTTACG GTCAGCTGCC TATAATCTTC TGTGTGCCTT AACTTGTACC
TTTAATTTAA AAATCGAGGG CCAGTTACTA GAGACATCAG GTTTATGTAT CCCTGCCAAC
AACACCCTCT TTATTGTCTC TATTAGTAAG ACACTGGCAG CCAATGAGCC ACACCTCACG
TTAGAATTTT TGGAAGAGTG TATTTCTGGA TTTAGCAAAT CTAGTATTGA ATTGAAACAC
CTTTGTTTGG AATACATGAC TCCATGGCTG TCAAATCTAG TTCGTTTTTG CAAGCATAAT
GATGATGCCA AACGACAAAG AGTTACTGCT ATTCTTGACA AGCTGATAAC AATGACCATC
AATGAAAAAC AGATGTACCC ATCTATTCAA GCAAAAATAT GGGGAAGCCT TGGGCAGATT
ACAGATCTGC TTGATGTTGT ACTAGACAGT TTCATCAAAA CCAGTGCAAC AGGTGGCTTG
GGATCAATAA AAGCTGAGGT GATGGCAGAT ACTGCTGTAG CTTTGGCTTC TGGAAATGTG
AAATTGGTTT CAAGCAAGGT TATTGGAAGG ATGTGCAAAA TAATTGACAA GACATGCTTA
TCTCCAACTC CTACTTTAGA ACAACATCTT ATGTGGGATG ATATTGCTAT TTTAGCACGC
TACATGCTGA TGCTGTCCTT CAACAATTCC CTTGATGTGG CAGCTCATCT TCCCTACCTC
TTCCACGTTG TTACTTTCTT AGTAGCCACA GGTCCGCTCT CCCTTAGAGC TTCCACACAT
GGACTGGTCA TTAATATCAT TCACTCTCTG TGTACTTGTT CACAGCTTCA TTTTAGTGAA
GAGACCAAGC AAGTTTTGAG ACTCAGTCTG ACAGAGTTCT CATTACCCAA ATTTTACTTG
CTGTTTGGCA TTAGCAAAGT CAAGTCAGCT GCTGTCATTG CCTTCCGTTC CAGTTACCGG
GACAGGTCAT TCTCTCCTGG CTCCTATGAG AGAGAGACTT TTGCTTTGAC ATCCTTGGAA
ACAGTCACAG AAGCTTTGTT GGAGATCATG GAGGCATGCA TGAGAGATAT TCCAACGTGC
AAGTGGCTGG ACCAGTGGAC AGAACTAGCT CAAAGATTTG CATTCCAATA TAATCCATCC
CTGCAACCAA GAGCTCTTGT TGTCTTTGGG TGTATTAGCA AACGAGTGTC TCATGGGCAG
ATAAAGCAGA TAATCCGTAT TCTTAGCAAG GCACTTGAGA GTTGCTTAAA AGGACCTGAC
ACTTACAACA GTCAAGTTCT GATAGAAGCT ACAGTAATAG CACTAACCAA ATTACAGCCA
CTTCTTAATA AGGACTCGCC TCTGCACAAA GCCCTCTTTT GGGTAGCTGT GGCTGTGCTG
CAGCTTGATG AGGTCAACTT GTATTCAGCA GGTACCGCAC TTCTTGAACA AAACCTGCAT
ACTTTAGATA GTCTCCGTAT ATTCAATGAC AAGAGTCCAG AGGAAGTATT TATGGCAATC
CGGAATCCTC TGGAGTGGCA CTGCAAGCAA ATGGATCATT TTGTTGGACT CAATTTCAAC
TCTAACTTTA ACTTTGCATT GGTTGGACAC CTTTTAAAAG GGTACAGGCA TCCTTCACCT
GCTATTGTTG CAAGAACAGT CAGAATTTTA CATACACTAC TAACTCTGGT TAACAAACAC
AGAAATTGTG ACAAATTTGA AGTGAATACA CAGAGCGTGG CCTACTTAGC AGCTTTACTT
ACAGTGTCTG AAGAAGTTCG AAGTCGCTGC AGCCTAAAAC ATAGAAAGTC ACTTCTTCTT
ACTGATATTT CAATGGAAAA TGTTCCTATG GATACATATC CCATTCATCA TGGTGACCCT
TCCTATAGGA CACTAAAGGA GACTCAGCCA TGGTCCTCTC CCAAAGGTTC TGAAGGATAC
CTTGCAGCCA CCTATCCAAC TGTCGGCCAG ACCAGTCCCC GAGCCAGGAA ATCCATGAGC
CTGGACATGG GGCAACCTTC TCAGGCCAAC ACTAAGAAGT TGCTTGGAAC AAGGAAAAGT
TTTGATCACT TGATATCAGA CACAAAGGCT CCTAAAAGGC AAGAAATGGA ATCAGGGATC
ACAACACCCC CCAAAATGAG GAGAGTAGCA GAAACTGATT ATGAAATGGA AACTCAGAGG
ATTTCCTCAT CACAACAGCA CCCACATTTA CGTAAAGTTT CAGTGTCTGA ATCAAATGTT
CTCTTGGATG AAGAAGTACT TACTGATCCG AAGATCCAGG CGCTGCTTCT TACTGTTCTA
GCTACACTGG TAAAATATAC CACAGATGAG TTTGATCAAC GAATTCTTTA TGAATACTTA
GCAGAGGCCA GTGTTGTGTT TCCCAAAGTC TTTCCTGTTG TGCATAATTT GTTGGACTCT
AAGATCAACA CCCTGTTATC ATTGTGCCAA GATCCAAATT TGTTAAATCC AATCCATGGA
ATTGTGCAGA GTGTGGTGTA CCATGAAGAA TCCCCACCAC AATACCAAAC ATCTTACCTG
CAAAGTTTTG GTTTTAATGG CTTGTGGCGG TTTGCAGGAC CGTTTTCAAA GCAAACACAA
ATTCCAGACT ATGCTGAGCT TATTGTTAAG TTTCTTGATG CCTTGATTGA CACGTACCTG
CCTGGAATTG ATGAAGAAAC CAGTGAAGAA TCCCTCCTGA CTCCCACATC TCCTTACCCT
CCTGCACTGC AGAGCCAGCT TAGTATCACT GCCAACCTTA ACCTTTCTAA TTCCATGACC
TCACTTGCAA CTTCCCAGCA TTCCCCAGCT TCTCTGCCTT GCTCTAAATC AGCAGTTTTC
ATGCAGCTGT TCCCTCATCA AGGAATCGAC AAGGAGAACG TTGAACTCTC CCCTACCACT
GGCCACTGTA ACAGTGGACG AACTCGCCAC GGATCCGCAA GCCAAGTGCA GAAGCAAAGA
AGCGCTGGCA GTTTCAAACG TAATAGCATT AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50429
Clone ID Related Accession (Same CDS sequence) XM_006721923 , XM_011524853 , XM_011524854 , XM_011524855 , XM_011524856
Accession Version XM_006721923.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8565bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neurofibromin isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578830992. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
ATGTGGAATG CAAAGGAGAG GCTTCCAATA AAAACAGGAC AGCAGAACAC ACATACCAAA 
GTCAGTACTG AGCACAACAA GGAATGTCTA ATCAATATTT CCAAATACAA GTTTTCTTTG
GTTATAAGCG GCCTCACTAC TATTTTAAAG AATGTTAACA ATATGAGAAT ATTTGGAGAA
GCTGCTGAAA AAAATTTATA TCTCTCTCAG TTGATTATAT TGGATACACT GGAAAAATGT
CTTGCTGGGC AACCAAAGGA CACAATGAGA TTAGATGAAA CGATGCTGGT CAAACAGTTG
CTGCCAGAAA TCTGCCATTT TCTTCACACC TGTCGTGAAG GAAACCAGCA TGCAGCTGAA
CTTCGGAATT CTGCCTCTGG GGTTTTATTT TCTCTCAGCT GCAACAACTT CAATGCAGTC
TTTAGTCGCA TTTCTACCAG GTTACAGGAA TTAACTGTTT GTTCAGAAGA CAATGTTGAT
GTTCATGATA TAGAATTGTT ACAGTATATC AATGTGGATT GTGCAAAATT AAAACGACTC
CTGAAGGAAA CAGCATTTAA ATTTAAAGCC CTAAAGAAGG TTGCGCAGTT AGCAGTTATA
AATAGCCTGG AAAAGGCATT TTGGAACTGG GTAGAAAATT ATCCAGATGA ATTTACAAAA
CTGTACCAGA TCCCACAGAC TGATATGGCT GAATGTGCAG AAAAGCTATT TGACTTGGTG
GATGGTTTTG CTGAAAGCAC CAAACGTAAA GCAGCAGTTT GGCCACTACA AATCATTCTC
CTTATCTTGT GTCCAGAAAT AATCCAGGAT ATATCCAAAG ACGTGGTTGA TGAAAACAAC
ATGAATAAGA AGTTATTTCT GGACAGTCTA CGAAAAGCTC TTGCTGGCCA TGGAGGAAGT
AGGCAGCTGA CAGAAAGTGC TGCAATTGCC TGTGTCAAAC TGTGTAAAGC AAGTACTTAC
ATCAATTGGG AAGATAACTC TGTCATTTTC CTACTTGTTC AGTCCATGGT GGTTGATCTT
AAGAACCTGC TTTTTAATCC AAGTAAGCCA TTCTCAAGAG GCAGTCAGCC TGCAGATGTG
GATCTAATGA TTGACTGCCT TGTTTCTTGC TTTCGTATAA GCCCTCACAA CAACCAACAC
TTTAAGATCT GCCTGGCTCA GAATTCACCT TCTACATTTC ACTATGTGCT GGTAAATTCA
CTCCATCGAA TCATCACCAA TAGCACTTTC AAGCATGGAC TTGGCACTGC TTCCGCATTG
GATTGGTGGC CTAAGATTGA TGCTGTGTAT TGTCACTCGG TTGAACTTCG AAATATGTTT
GGTGAAACAC TTCATAAAGC AGTGCAAGGT TGTGGAGCAC ACCCAGCAAT ACGAATGGCA
CCGAGTCTTA CATTTAAAGA AAAAGTAACA AGCCTTAAAT TTAAAGAAAA ACCTACAGAC
CTGGAGACAA GAAGCTATAA GTATCTTCTC TTGTCCATGG TGAAACTAAT TCATGCAGAT
CCAAAGCTCT TGCTTTGTAA TCCAAGAAAA CAGGGGCCCG AAACCCAAGG CAGTACAGCA
GAATTAATTA CAGGGCTCGT CCAACTGGTC CCTCAGTCAC ACATGCCAGA GATTGCTCAG
GAAGCAATGG AGGCTCTGCT GGTTCTTCAT CAGTTAGATA GCATTGATTT GTGGAATCCT
GATGCTCCTG TAGAAACATT TTGGGAGATT AGCTCACAAA TGCTTTTTTA CATCTGCAAG
AAATTAACTA GTCATCAAAT GCTTAGTAGC ACAGAAATTC TCAAGTGGTT GCGGGAAATA
TTGATCTGCA GGAATAAATT TCTTCTTAAA AATAAGCAGG CAGATAGAAG TTCCTGTCAC
TTTCTCCTTT TTTACGGGGT AGGATGTGAT ATTCCTTCTA GTGGAAATAC CAGTCAAATG
TCCATGGATC ATGAAGAATT ACTACGTACT CCTGGAGCCT CTCTCCGGAA GGGAAAAGGG
AACTCCTCTA TGGATAGTGC AGCAGGATGC AGCGGAACCC CCCCGATTTG CCGACAAGCC
CAGACCAAAC TAGAAGTGGC CCTGTACATG TTTCTGTGGA ACCCTGACAC TGAAGCTGTT
CTGGTTGCCA TGTCCTGTTT CCGCCACCTC TGTGAGGAAG CAGATATCCG GTGTGGGGTG
GATGAAGTGT CAGTGCATAA CCTCTTGCCC AACTATAACA CATTCATGGA GTTTGCCTCT
GTCAGCAATA TGATGTCAAC AGGAAGAGCA GCACTTCAGA AAAGAGTGAT GGCACTGCTG
AGGCGCATTG AGCATCCCAC TGCAGGAAAC ACTGAGGCTT GGGAAGATAC ACATGCAAAA
TGGGAACAAG CAACAAAGCT AATCCTTAAC TATCCAAAAG CCAAAATGGA AGATGGCCAG
GCTGCTGAAA GCCTTCACAA GACCATTGTT AAGAGGCGAA TGTCCCATGT GAGTGGAGGA
GGATCCATAG ATTTGTCTGA CACAGACTCC CTACAGGAAT GGATCAACAT GACTGGCTTC
CTTTGTGCCC TTGGGGGAGT GTGCCTCCAG CAGAGAAGCA ATTCTGGCCT GGCAACCTAT
AGCCCACCCA TGGGTCCAGT CAGTGAACGT AAGGGTTCTA TGATTTCAGT GATGTCTTCA
GAGGGAAACG CAGATACACC TGTCAGCAAA TTTATGGATC GGCTGTTGTC CTTAATGGTG
TGTAACCATG AGAAAGTGGG ACTTCAAATA CGGACCAATG TTAAGGATCT GGTGGGTCTA
GAATTGAGTC CTGCTCTGTA TCCAATGCTA TTTAACAAAT TGAAGAATAC CATCAGCAAG
TTTTTTGACT CCCAAGGACA GGTTTTATTG ACTGATACCA ATACTCAATT TGTAGAACAA
ACCATAGCTA TAATGAAGAA CTTGCTAGAT AATCATACTG AAGGCAGCTC TGAACATCTA
GGGCAAGCTA GCATTGAAAC AATGATGTTA AATCTGGTCA GGTATGTTCG TGTGCTTGGG
AATATGGTCC ATGCAATTCA AATAAAAACG AAACTGTGTC AATTAGTTGA AGTAATGATG
GCAAGGAGAG ATGACCTCTC ATTTTGCCAA GAGATGAAAT TTAGGAATAA GATGGTAGAA
TACCTGACAG ACTGGGTTAT GGGAACATCA AACCAAGCAG CAGATGATGA TGTAAAATGT
CTTACAAGAG ATTTGGACCA GGCAAGCATG GAAGCAGTAG TTTCACTTCT AGCTGGTCTC
CCTCTGCAGC CTGAAGAAGG AGATGGTGTG GAATTGATGG AAGCCAAATC ACAGTTATTT
CTTAAATACT TCACATTATT TATGAACCTT TTGAATGACT GCAGTGAAGT TGAAGATGAA
AGTGCGCAAA CAGGTGGCAG GAAACGTGGC ATGTCTCGGA GGCTGGCATC ACTGAGGCAC
TGTACGGTCC TTGCAATGTC AAACTTACTC AATGCCAACG TAGACAGTGG TCTCATGCAC
TCCATAGGCT TAGGTTACCA CAAGGATCTC CAGACAAGAG CTACATTTAT GGAAGTTCTG
ACAAAAATCC TTCAACAAGG CACAGAATTT GACACACTTG CAGAAACAGT ATTGGCTGAT
CGGTTTGAGA GATTGGTGGA ACTGGTCACA ATGATGGGTG ATCAAGGAGA ACTCCCTATA
GCGATGGCTC TGGCCAATGT GGTTCCTTGT TCTCAGTGGG ATGAACTAGC TCGAGTTCTG
GTTACTCTGT TTGATTCTCG GCATTTACTC TACCAACTGC TCTGGAACAT GTTTTCTAAA
GAAGTAGAAT TGGCAGACTC CATGCAGACT CTCTTCCGAG GCAACAGCTT GGCCAGTAAA
ATAATGACAT TCTGTTTCAA GGTATATGGT GCTACCTATC TACAAAAACT CCTGGATCCT
TTATTACGAA TTGTGATCAC ATCCTCTGAT TGGCAACATG TTAGCTTTGA AGTGGATCCT
ACCAGGTTAG AACCATCAGA GAGCCTTGAG GAAAACCAGC GGAACCTCCT TCAGATGACT
GAAAAGTTCT TCCATGCCAT CATCAGTTCC TCCTCAGAAT TCCCCCCTCA ACTTCGAAGT
GTGTGCCACT GTTTATACCA GGCAACTTGC CACTCCCTAC TGAATAAAGC TACAGTAAAA
GAAAAAAAGG AAAACAAAAA ATCAGTGGTT AGCCAGCGTT TCCCTCAGAA CAGCATCGGT
GCAGTAGGAA GTGCCATGTT CCTCAGATTT ATCAATCCTG CCATTGTCTC ACCGTATGAA
GCAGGGATTT TAGATAAAAA GCCACCACCT AGAATCGAAA GGGGCTTGAA GTTAATGTCA
AAGATACTTC AGAGTATTGC CAATCATGTT CTCTTCACAA AAGAAGAACA TATGCGGCCT
TTCAATGATT TTGTGAAAAG CAACTTTGAT GCAGCACGCA GGTTTTTCCT TGATATAGCA
TCTGATTGTC CTACAAGTGA TGCAGTAAAT CATAGTCTTT CCTTCATAAG TGACGGCAAT
GTGCTTGCTT TACATCGTCT ACTCTGGAAC AATCAGGAGA AAATTGGGCA GTATCTTTCC
AGCAACAGGG ATCATAAAGC TGTTGGAAGA CGACCTTTTG ATAAGATGGC AACACTTCTT
GCATACCTGG GTCCTCCAGA GCACAAACCT GTGGCAGATA CACACTGGTC CAGCCTTAAC
CTTACCAGTT CAAAGTTTGA GGAATTTATG ACTAGGCATC AGGTACATGA AAAAGAAGAA
TTCAAGGCTT TGAAAACGTT AAGTATTTTC TACCAAGCTG GGACTTCCAA AGCTGGGAAT
CCTATTTTTT ATTATGTTGC ACGGAGGTTC AAAACTGGTC AAATCAATGG TGATTTGCTG
ATATACCATG TCTTACTGAC TTTAAAGCCA TATTATGCAA AGCCATATGA AATTGTAGTG
GACCTTACCC ATACCGGGCC TAGCAATCGC TTTAAAACAG ACTTTCTCTC TAAGTGGTTT
GTTGTTTTTC CTGGCTTTGC TTACGACAAC GTCTCCGCAG TCTATATCTA TAACTGTAAC
TCCTGGGTCA GGGAGTACAC CAAGTATCAT GAGCGGCTGC TGACTGGCCT CAAAGGTAGC
AAAAGGCTTG TTTTCATAGA CTGTCCTGGG AAACTGGCTG AGCACATAGA GCATGAACAA
CAGAAACTAC CTGCTGCCAC CTTGGCTTTA GAAGAGGACC TGAAGGTATT CCACAATGCT
CTCAAGCTAG CTCACAAAGA CACCAAAGTT TCTATTAAAG TTGGTTCTAC TGCTGTCCAA
GTAACTTCAG CAGAGCGAAC AAAAGTCCTA GGGCAATCAG TCTTTCTAAA TGACATTTAT
TATGCTTCGG AAATTGAAGA AATCTGCCTA GTAGATGAGA ACCAGTTCAC CTTAACCATT
GCAAACCAGG GCACGCCGCT CACCTTCATG CACCAGGAGT GTGAAGCCAT TGTCCAGTCT
ATCATTCATA TCCGGACCCG CTGGGAACTG TCACAGCCCG ACTCTATCCC CCAACACACC
AAGATTCGGC CAAAAGATGT CCCTGGGACA CTGCTCAATA TCGCATTACT TAATTTAGGC
AGTTCTGACC CGAGTTTACG GTCAGCTGCC TATAATCTTC TGTGTGCCTT AACTTGTACC
TTTAATTTAA AAATCGAGGG CCAGTTACTA GAGACATCAG GTTTATGTAT CCCTGCCAAC
AACACCCTCT TTATTGTCTC TATTAGTAAG ACACTGGCAG CCAATGAGCC ACACCTCACG
TTAGAATTTT TGGAAGAGTG TATTTCTGGA TTTAGCAAAT CTAGTATTGA ATTGAAACAC
CTTTGTTTGG AATACATGAC TCCATGGCTG TCAAATCTAG TTCGTTTTTG CAAGCATAAT
GATGATGCCA AACGACAAAG AGTTACTGCT ATTCTTGACA AGCTGATAAC AATGACCATC
AATGAAAAAC AGATGTACCC ATCTATTCAA GCAAAAATAT GGGGAAGCCT TGGGCAGATT
ACAGATCTGC TTGATGTTGT ACTAGACAGT TTCATCAAAA CCAGTGCAAC AGGTGGCTTG
GGATCAATAA AAGCTGAGGT GATGGCAGAT ACTGCTGTAG CTTTGGCTTC TGGAAATGTG
AAATTGGTTT CAAGCAAGGT TATTGGAAGG ATGTGCAAAA TAATTGACAA GACATGCTTA
TCTCCAACTC CTACTTTAGA ACAACATCTT ATGTGGGATG ATATTGCTAT TTTAGCACGC
TACATGCTGA TGCTGTCCTT CAACAATTCC CTTGATGTGG CAGCTCATCT TCCCTACCTC
TTCCACGTTG TTACTTTCTT AGTAGCCACA GGTCCGCTCT CCCTTAGAGC TTCCACACAT
GGACTGGTCA TTAATATCAT TCACTCTCTG TGTACTTGTT CACAGCTTCA TTTTAGTGAA
GAGACCAAGC AAGTTTTGAG ACTCAGTCTG ACAGAGTTCT CATTACCCAA ATTTTACTTG
CTGTTTGGCA TTAGCAAAGT CAAGTCAGCT GCTGTCATTG CCTTCCGTTC CAGTTACCGG
GACAGGTCAT TCTCTCCTGG CTCCTATGAG AGAGAGACTT TTGCTTTGAC ATCCTTGGAA
ACAGTCACAG AAGCTTTGTT GGAGATCATG GAGGCATGCA TGAGAGATAT TCCAACGTGC
AAGTGGCTGG ACCAGTGGAC AGAACTAGCT CAAAGATTTG CATTCCAATA TAATCCATCC
CTGCAACCAA GAGCTCTTGT TGTCTTTGGG TGTATTAGCA AACGAGTGTC TCATGGGCAG
ATAAAGCAGA TAATCCGTAT TCTTAGCAAG GCACTTGAGA GTTGCTTAAA AGGACCTGAC
ACTTACAACA GTCAAGTTCT GATAGAAGCT ACAGTAATAG CACTAACCAA ATTACAGCCA
CTTCTTAATA AGGACTCGCC TCTGCACAAA GCCCTCTTTT GGGTAGCTGT GGCTGTGCTG
CAGCTTGATG AGGTCAACTT GTATTCAGCA GGTACCGCAC TTCTTGAACA AAACCTGCAT
ACTTTAGATA GTCTCCGTAT ATTCAATGAC AAGAGTCCAG AGGAAGTATT TATGGCAATC
CGGAATCCTC TGGAGTGGCA CTGCAAGCAA ATGGATCATT TTGTTGGACT CAATTTCAAC
TCTAACTTTA ACTTTGCATT GGTTGGACAC CTTTTAAAAG GGTACAGGCA TCCTTCACCT
GCTATTGTTG CAAGAACAGT CAGAATTTTA CATACACTAC TAACTCTGGT TAACAAACAC
AGAAATTGTG ACAAATTTGA AGTGAATACA CAGAGCGTGG CCTACTTAGC AGCTTTACTT
ACAGTGTCTG AAGAAGTTCG AAGTCGCTGC AGCCTAAAAC ATAGAAAGTC ACTTCTTCTT
ACTGATATTT CAATGGAAAA TGTTCCTATG GATACATATC CCATTCATCA TGGTGACCCT
TCCTATAGGA CACTAAAGGA GACTCAGCCA TGGTCCTCTC CCAAAGGTTC TGAAGGATAC
CTTGCAGCCA CCTATCCAAC TGTCGGCCAG ACCAGTCCCC GAGCCAGGAA ATCCATGAGC
CTGGACATGG GGCAACCTTC TCAGGCCAAC ACTAAGAAGT TGCTTGGAAC AAGGAAAAGT
TTTGATCACT TGATATCAGA CACAAAGGCT CCTAAAAGGC AAGAAATGGA ATCAGGGATC
ACAACACCCC CCAAAATGAG GAGAGTAGCA GAAACTGATT ATGAAATGGA AACTCAGAGG
ATTTCCTCAT CACAACAGCA CCCACATTTA CGTAAAGTTT CAGTGTCTGA ATCAAATGTT
CTCTTGGATG AAGAAGTACT TACTGATCCG AAGATCCAGG CGCTGCTTCT TACTGTTCTA
GCTACACTGG TAAAATATAC CACAGATGAG TTTGATCAAC GAATTCTTTA TGAATACTTA
GCAGAGGCCA GTGTTGTGTT TCCCAAAGTC TTTCCTGTTG TGCATAATTT GTTGGACTCT
AAGATCAACA CCCTGTTATC ATTGTGCCAA GATCCAAATT TGTTAAATCC AATCCATGGA
ATTGTGCAGA GTGTGGTGTA CCATGAAGAA TCCCCACCAC AATACCAAAC ATCTTACCTG
CAAAGTTTTG GTTTTAATGG CTTGTGGCGG TTTGCAGGAC CGTTTTCAAA GCAAACACAA
ATTCCAGACT ATGCTGAGCT TATTGTTAAG TTTCTTGATG CCTTGATTGA CACGTACCTG
CCTGGAATTG ATGAAGAAAC CAGTGAAGAA TCCCTCCTGA CTCCCACATC TCCTTACCCT
CCTGCACTGC AGAGCCAGCT TAGTATCACT GCCAACCTTA ACCTTTCTAA TTCCATGACC
TCACTTGCAA CTTCCCAGCA TTCCCCAGCT TCTCTGCCTT GCTCTAAATC AGCAGTTTTC
ATGCAGCTGT TCCCTCATCA AGGAATCGAC AAGGAGAACG TTGAACTCTC CCCTACCACT
GGCCACTGTA ACAGTGGACG AACTCGCCAC GGATCCGCAA GCCAAGTGCA GAAGCAAAGA
AGCGCTGGCA GTTTCAAACG TAATAGCATT AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006721986.1
CDS640..9204
Misc Feature(1)4189..5301(+)
Misc Feature(2)4222..5283(+)
Misc Feature(3)4336..5004(+)
Misc Feature(4)5371..5814(+)
Misc Feature(5)5440..5745(+)
Misc Feature(6)5644..5742(+)
Misc Feature(7)5812..6141(+)
Misc Feature(8)5812..6141(+)
Translation

Target ORF information:

RefSeq Version XM_006721923
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006721923

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
ATGTGGAATG CAAAGGAGAG GCTTCCAATA AAAACAGGAC AGCAGAACAC ACATACCAAA 
GTCAGTACTG AGCACAACAA GGAATGTCTA ATCAATATTT CCAAATACAA GTTTTCTTTG
GTTATAAGCG GCCTCACTAC TATTTTAAAG AATGTTAACA ATATGAGAAT ATTTGGAGAA
GCTGCTGAAA AAAATTTATA TCTCTCTCAG TTGATTATAT TGGATACACT GGAAAAATGT
CTTGCTGGGC AACCAAAGGA CACAATGAGA TTAGATGAAA CGATGCTGGT CAAACAGTTG
CTGCCAGAAA TCTGCCATTT TCTTCACACC TGTCGTGAAG GAAACCAGCA TGCAGCTGAA
CTTCGGAATT CTGCCTCTGG GGTTTTATTT TCTCTCAGCT GCAACAACTT CAATGCAGTC
TTTAGTCGCA TTTCTACCAG GTTACAGGAA TTAACTGTTT GTTCAGAAGA CAATGTTGAT
GTTCATGATA TAGAATTGTT ACAGTATATC AATGTGGATT GTGCAAAATT AAAACGACTC
CTGAAGGAAA CAGCATTTAA ATTTAAAGCC CTAAAGAAGG TTGCGCAGTT AGCAGTTATA
AATAGCCTGG AAAAGGCATT TTGGAACTGG GTAGAAAATT ATCCAGATGA ATTTACAAAA
CTGTACCAGA TCCCACAGAC TGATATGGCT GAATGTGCAG AAAAGCTATT TGACTTGGTG
GATGGTTTTG CTGAAAGCAC CAAACGTAAA GCAGCAGTTT GGCCACTACA AATCATTCTC
CTTATCTTGT GTCCAGAAAT AATCCAGGAT ATATCCAAAG ACGTGGTTGA TGAAAACAAC
ATGAATAAGA AGTTATTTCT GGACAGTCTA CGAAAAGCTC TTGCTGGCCA TGGAGGAAGT
AGGCAGCTGA CAGAAAGTGC TGCAATTGCC TGTGTCAAAC TGTGTAAAGC AAGTACTTAC
ATCAATTGGG AAGATAACTC TGTCATTTTC CTACTTGTTC AGTCCATGGT GGTTGATCTT
AAGAACCTGC TTTTTAATCC AAGTAAGCCA TTCTCAAGAG GCAGTCAGCC TGCAGATGTG
GATCTAATGA TTGACTGCCT TGTTTCTTGC TTTCGTATAA GCCCTCACAA CAACCAACAC
TTTAAGATCT GCCTGGCTCA GAATTCACCT TCTACATTTC ACTATGTGCT GGTAAATTCA
CTCCATCGAA TCATCACCAA TAGCACTTTC AAGCATGGAC TTGGCACTGC TTCCGCATTG
GATTGGTGGC CTAAGATTGA TGCTGTGTAT TGTCACTCGG TTGAACTTCG AAATATGTTT
GGTGAAACAC TTCATAAAGC AGTGCAAGGT TGTGGAGCAC ACCCAGCAAT ACGAATGGCA
CCGAGTCTTA CATTTAAAGA AAAAGTAACA AGCCTTAAAT TTAAAGAAAA ACCTACAGAC
CTGGAGACAA GAAGCTATAA GTATCTTCTC TTGTCCATGG TGAAACTAAT TCATGCAGAT
CCAAAGCTCT TGCTTTGTAA TCCAAGAAAA CAGGGGCCCG AAACCCAAGG CAGTACAGCA
GAATTAATTA CAGGGCTCGT CCAACTGGTC CCTCAGTCAC ACATGCCAGA GATTGCTCAG
GAAGCAATGG AGGCTCTGCT GGTTCTTCAT CAGTTAGATA GCATTGATTT GTGGAATCCT
GATGCTCCTG TAGAAACATT TTGGGAGATT AGCTCACAAA TGCTTTTTTA CATCTGCAAG
AAATTAACTA GTCATCAAAT GCTTAGTAGC ACAGAAATTC TCAAGTGGTT GCGGGAAATA
TTGATCTGCA GGAATAAATT TCTTCTTAAA AATAAGCAGG CAGATAGAAG TTCCTGTCAC
TTTCTCCTTT TTTACGGGGT AGGATGTGAT ATTCCTTCTA GTGGAAATAC CAGTCAAATG
TCCATGGATC ATGAAGAATT ACTACGTACT CCTGGAGCCT CTCTCCGGAA GGGAAAAGGG
AACTCCTCTA TGGATAGTGC AGCAGGATGC AGCGGAACCC CCCCGATTTG CCGACAAGCC
CAGACCAAAC TAGAAGTGGC CCTGTACATG TTTCTGTGGA ACCCTGACAC TGAAGCTGTT
CTGGTTGCCA TGTCCTGTTT CCGCCACCTC TGTGAGGAAG CAGATATCCG GTGTGGGGTG
GATGAAGTGT CAGTGCATAA CCTCTTGCCC AACTATAACA CATTCATGGA GTTTGCCTCT
GTCAGCAATA TGATGTCAAC AGGAAGAGCA GCACTTCAGA AAAGAGTGAT GGCACTGCTG
AGGCGCATTG AGCATCCCAC TGCAGGAAAC ACTGAGGCTT GGGAAGATAC ACATGCAAAA
TGGGAACAAG CAACAAAGCT AATCCTTAAC TATCCAAAAG CCAAAATGGA AGATGGCCAG
GCTGCTGAAA GCCTTCACAA GACCATTGTT AAGAGGCGAA TGTCCCATGT GAGTGGAGGA
GGATCCATAG ATTTGTCTGA CACAGACTCC CTACAGGAAT GGATCAACAT GACTGGCTTC
CTTTGTGCCC TTGGGGGAGT GTGCCTCCAG CAGAGAAGCA ATTCTGGCCT GGCAACCTAT
AGCCCACCCA TGGGTCCAGT CAGTGAACGT AAGGGTTCTA TGATTTCAGT GATGTCTTCA
GAGGGAAACG CAGATACACC TGTCAGCAAA TTTATGGATC GGCTGTTGTC CTTAATGGTG
TGTAACCATG AGAAAGTGGG ACTTCAAATA CGGACCAATG TTAAGGATCT GGTGGGTCTA
GAATTGAGTC CTGCTCTGTA TCCAATGCTA TTTAACAAAT TGAAGAATAC CATCAGCAAG
TTTTTTGACT CCCAAGGACA GGTTTTATTG ACTGATACCA ATACTCAATT TGTAGAACAA
ACCATAGCTA TAATGAAGAA CTTGCTAGAT AATCATACTG AAGGCAGCTC TGAACATCTA
GGGCAAGCTA GCATTGAAAC AATGATGTTA AATCTGGTCA GGTATGTTCG TGTGCTTGGG
AATATGGTCC ATGCAATTCA AATAAAAACG AAACTGTGTC AATTAGTTGA AGTAATGATG
GCAAGGAGAG ATGACCTCTC ATTTTGCCAA GAGATGAAAT TTAGGAATAA GATGGTAGAA
TACCTGACAG ACTGGGTTAT GGGAACATCA AACCAAGCAG CAGATGATGA TGTAAAATGT
CTTACAAGAG ATTTGGACCA GGCAAGCATG GAAGCAGTAG TTTCACTTCT AGCTGGTCTC
CCTCTGCAGC CTGAAGAAGG AGATGGTGTG GAATTGATGG AAGCCAAATC ACAGTTATTT
CTTAAATACT TCACATTATT TATGAACCTT TTGAATGACT GCAGTGAAGT TGAAGATGAA
AGTGCGCAAA CAGGTGGCAG GAAACGTGGC ATGTCTCGGA GGCTGGCATC ACTGAGGCAC
TGTACGGTCC TTGCAATGTC AAACTTACTC AATGCCAACG TAGACAGTGG TCTCATGCAC
TCCATAGGCT TAGGTTACCA CAAGGATCTC CAGACAAGAG CTACATTTAT GGAAGTTCTG
ACAAAAATCC TTCAACAAGG CACAGAATTT GACACACTTG CAGAAACAGT ATTGGCTGAT
CGGTTTGAGA GATTGGTGGA ACTGGTCACA ATGATGGGTG ATCAAGGAGA ACTCCCTATA
GCGATGGCTC TGGCCAATGT GGTTCCTTGT TCTCAGTGGG ATGAACTAGC TCGAGTTCTG
GTTACTCTGT TTGATTCTCG GCATTTACTC TACCAACTGC TCTGGAACAT GTTTTCTAAA
GAAGTAGAAT TGGCAGACTC CATGCAGACT CTCTTCCGAG GCAACAGCTT GGCCAGTAAA
ATAATGACAT TCTGTTTCAA GGTATATGGT GCTACCTATC TACAAAAACT CCTGGATCCT
TTATTACGAA TTGTGATCAC ATCCTCTGAT TGGCAACATG TTAGCTTTGA AGTGGATCCT
ACCAGGTTAG AACCATCAGA GAGCCTTGAG GAAAACCAGC GGAACCTCCT TCAGATGACT
GAAAAGTTCT TCCATGCCAT CATCAGTTCC TCCTCAGAAT TCCCCCCTCA ACTTCGAAGT
GTGTGCCACT GTTTATACCA GGCAACTTGC CACTCCCTAC TGAATAAAGC TACAGTAAAA
GAAAAAAAGG AAAACAAAAA ATCAGTGGTT AGCCAGCGTT TCCCTCAGAA CAGCATCGGT
GCAGTAGGAA GTGCCATGTT CCTCAGATTT ATCAATCCTG CCATTGTCTC ACCGTATGAA
GCAGGGATTT TAGATAAAAA GCCACCACCT AGAATCGAAA GGGGCTTGAA GTTAATGTCA
AAGATACTTC AGAGTATTGC CAATCATGTT CTCTTCACAA AAGAAGAACA TATGCGGCCT
TTCAATGATT TTGTGAAAAG CAACTTTGAT GCAGCACGCA GGTTTTTCCT TGATATAGCA
TCTGATTGTC CTACAAGTGA TGCAGTAAAT CATAGTCTTT CCTTCATAAG TGACGGCAAT
GTGCTTGCTT TACATCGTCT ACTCTGGAAC AATCAGGAGA AAATTGGGCA GTATCTTTCC
AGCAACAGGG ATCATAAAGC TGTTGGAAGA CGACCTTTTG ATAAGATGGC AACACTTCTT
GCATACCTGG GTCCTCCAGA GCACAAACCT GTGGCAGATA CACACTGGTC CAGCCTTAAC
CTTACCAGTT CAAAGTTTGA GGAATTTATG ACTAGGCATC AGGTACATGA AAAAGAAGAA
TTCAAGGCTT TGAAAACGTT AAGTATTTTC TACCAAGCTG GGACTTCCAA AGCTGGGAAT
CCTATTTTTT ATTATGTTGC ACGGAGGTTC AAAACTGGTC AAATCAATGG TGATTTGCTG
ATATACCATG TCTTACTGAC TTTAAAGCCA TATTATGCAA AGCCATATGA AATTGTAGTG
GACCTTACCC ATACCGGGCC TAGCAATCGC TTTAAAACAG ACTTTCTCTC TAAGTGGTTT
GTTGTTTTTC CTGGCTTTGC TTACGACAAC GTCTCCGCAG TCTATATCTA TAACTGTAAC
TCCTGGGTCA GGGAGTACAC CAAGTATCAT GAGCGGCTGC TGACTGGCCT CAAAGGTAGC
AAAAGGCTTG TTTTCATAGA CTGTCCTGGG AAACTGGCTG AGCACATAGA GCATGAACAA
CAGAAACTAC CTGCTGCCAC CTTGGCTTTA GAAGAGGACC TGAAGGTATT CCACAATGCT
CTCAAGCTAG CTCACAAAGA CACCAAAGTT TCTATTAAAG TTGGTTCTAC TGCTGTCCAA
GTAACTTCAG CAGAGCGAAC AAAAGTCCTA GGGCAATCAG TCTTTCTAAA TGACATTTAT
TATGCTTCGG AAATTGAAGA AATCTGCCTA GTAGATGAGA ACCAGTTCAC CTTAACCATT
GCAAACCAGG GCACGCCGCT CACCTTCATG CACCAGGAGT GTGAAGCCAT TGTCCAGTCT
ATCATTCATA TCCGGACCCG CTGGGAACTG TCACAGCCCG ACTCTATCCC CCAACACACC
AAGATTCGGC CAAAAGATGT CCCTGGGACA CTGCTCAATA TCGCATTACT TAATTTAGGC
AGTTCTGACC CGAGTTTACG GTCAGCTGCC TATAATCTTC TGTGTGCCTT AACTTGTACC
TTTAATTTAA AAATCGAGGG CCAGTTACTA GAGACATCAG GTTTATGTAT CCCTGCCAAC
AACACCCTCT TTATTGTCTC TATTAGTAAG ACACTGGCAG CCAATGAGCC ACACCTCACG
TTAGAATTTT TGGAAGAGTG TATTTCTGGA TTTAGCAAAT CTAGTATTGA ATTGAAACAC
CTTTGTTTGG AATACATGAC TCCATGGCTG TCAAATCTAG TTCGTTTTTG CAAGCATAAT
GATGATGCCA AACGACAAAG AGTTACTGCT ATTCTTGACA AGCTGATAAC AATGACCATC
AATGAAAAAC AGATGTACCC ATCTATTCAA GCAAAAATAT GGGGAAGCCT TGGGCAGATT
ACAGATCTGC TTGATGTTGT ACTAGACAGT TTCATCAAAA CCAGTGCAAC AGGTGGCTTG
GGATCAATAA AAGCTGAGGT GATGGCAGAT ACTGCTGTAG CTTTGGCTTC TGGAAATGTG
AAATTGGTTT CAAGCAAGGT TATTGGAAGG ATGTGCAAAA TAATTGACAA GACATGCTTA
TCTCCAACTC CTACTTTAGA ACAACATCTT ATGTGGGATG ATATTGCTAT TTTAGCACGC
TACATGCTGA TGCTGTCCTT CAACAATTCC CTTGATGTGG CAGCTCATCT TCCCTACCTC
TTCCACGTTG TTACTTTCTT AGTAGCCACA GGTCCGCTCT CCCTTAGAGC TTCCACACAT
GGACTGGTCA TTAATATCAT TCACTCTCTG TGTACTTGTT CACAGCTTCA TTTTAGTGAA
GAGACCAAGC AAGTTTTGAG ACTCAGTCTG ACAGAGTTCT CATTACCCAA ATTTTACTTG
CTGTTTGGCA TTAGCAAAGT CAAGTCAGCT GCTGTCATTG CCTTCCGTTC CAGTTACCGG
GACAGGTCAT TCTCTCCTGG CTCCTATGAG AGAGAGACTT TTGCTTTGAC ATCCTTGGAA
ACAGTCACAG AAGCTTTGTT GGAGATCATG GAGGCATGCA TGAGAGATAT TCCAACGTGC
AAGTGGCTGG ACCAGTGGAC AGAACTAGCT CAAAGATTTG CATTCCAATA TAATCCATCC
CTGCAACCAA GAGCTCTTGT TGTCTTTGGG TGTATTAGCA AACGAGTGTC TCATGGGCAG
ATAAAGCAGA TAATCCGTAT TCTTAGCAAG GCACTTGAGA GTTGCTTAAA AGGACCTGAC
ACTTACAACA GTCAAGTTCT GATAGAAGCT ACAGTAATAG CACTAACCAA ATTACAGCCA
CTTCTTAATA AGGACTCGCC TCTGCACAAA GCCCTCTTTT GGGTAGCTGT GGCTGTGCTG
CAGCTTGATG AGGTCAACTT GTATTCAGCA GGTACCGCAC TTCTTGAACA AAACCTGCAT
ACTTTAGATA GTCTCCGTAT ATTCAATGAC AAGAGTCCAG AGGAAGTATT TATGGCAATC
CGGAATCCTC TGGAGTGGCA CTGCAAGCAA ATGGATCATT TTGTTGGACT CAATTTCAAC
TCTAACTTTA ACTTTGCATT GGTTGGACAC CTTTTAAAAG GGTACAGGCA TCCTTCACCT
GCTATTGTTG CAAGAACAGT CAGAATTTTA CATACACTAC TAACTCTGGT TAACAAACAC
AGAAATTGTG ACAAATTTGA AGTGAATACA CAGAGCGTGG CCTACTTAGC AGCTTTACTT
ACAGTGTCTG AAGAAGTTCG AAGTCGCTGC AGCCTAAAAC ATAGAAAGTC ACTTCTTCTT
ACTGATATTT CAATGGAAAA TGTTCCTATG GATACATATC CCATTCATCA TGGTGACCCT
TCCTATAGGA CACTAAAGGA GACTCAGCCA TGGTCCTCTC CCAAAGGTTC TGAAGGATAC
CTTGCAGCCA CCTATCCAAC TGTCGGCCAG ACCAGTCCCC GAGCCAGGAA ATCCATGAGC
CTGGACATGG GGCAACCTTC TCAGGCCAAC ACTAAGAAGT TGCTTGGAAC AAGGAAAAGT
TTTGATCACT TGATATCAGA CACAAAGGCT CCTAAAAGGC AAGAAATGGA ATCAGGGATC
ACAACACCCC CCAAAATGAG GAGAGTAGCA GAAACTGATT ATGAAATGGA AACTCAGAGG
ATTTCCTCAT CACAACAGCA CCCACATTTA CGTAAAGTTT CAGTGTCTGA ATCAAATGTT
CTCTTGGATG AAGAAGTACT TACTGATCCG AAGATCCAGG CGCTGCTTCT TACTGTTCTA
GCTACACTGG TAAAATATAC CACAGATGAG TTTGATCAAC GAATTCTTTA TGAATACTTA
GCAGAGGCCA GTGTTGTGTT TCCCAAAGTC TTTCCTGTTG TGCATAATTT GTTGGACTCT
AAGATCAACA CCCTGTTATC ATTGTGCCAA GATCCAAATT TGTTAAATCC AATCCATGGA
ATTGTGCAGA GTGTGGTGTA CCATGAAGAA TCCCCACCAC AATACCAAAC ATCTTACCTG
CAAAGTTTTG GTTTTAATGG CTTGTGGCGG TTTGCAGGAC CGTTTTCAAA GCAAACACAA
ATTCCAGACT ATGCTGAGCT TATTGTTAAG TTTCTTGATG CCTTGATTGA CACGTACCTG
CCTGGAATTG ATGAAGAAAC CAGTGAAGAA TCCCTCCTGA CTCCCACATC TCCTTACCCT
CCTGCACTGC AGAGCCAGCT TAGTATCACT GCCAACCTTA ACCTTTCTAA TTCCATGACC
TCACTTGCAA CTTCCCAGCA TTCCCCAGCT TCTCTGCCTT GCTCTAAATC AGCAGTTTTC
ATGCAGCTGT TCCCTCATCA AGGAATCGAC AAGGAGAACG TTGAACTCTC CCCTACCACT
GGCCACTGTA ACAGTGGACG AACTCGCCAC GGATCCGCAA GCCAAGTGCA GAAGCAAAGA
AGCGCTGGCA GTTTCAAACG TAATAGCATT AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50430
Clone ID Related Accession (Same CDS sequence) XM_006721924
Accession Version XM_006721924.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8550bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neurofibromin isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
CGGAGGTTCA AAACTGGTCA AATCAATGGT GATTTGCTGA TATACCATGT CTTACTGACT
TTAAAGCCAT ATTATGCAAA GCCATATGAA ATTGTAGTGG ACCTTACCCA TACCGGGCCT
AGCAATCGCT TTAAAACAGA CTTTCTCTCT AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT
TACGACAACG TCTCCGCAGT CTATATCTAT AACTGTAACT CCTGGGTCAG GGAGTACACC
AAGTATCATG AGCGGCTGCT GACTGGCCTC AAAGGTAGCA AAAGGCTTGT TTTCATAGAC
TGTCCTGGGA AACTGGCTGA GCACATAGAG CATGAACAAC AGAAACTACC TGCTGCCACC
TTGGCTTTAG AAGAGGACCT GAAGGTATTC CACAATGCTC TCAAGCTAGC TCACAAAGAC
ACCAAAGTTT CTATTAAAGT TGGTTCTACT GCTGTCCAAG TAACTTCAGC AGAGCGAACA
AAAGTCCTAG GGCAATCAGT CTTTCTAAAT GACATTTATT ATGCTTCGGA AATTGAAGAA
ATCTGCCTAG TAGATGAGAA CCAGTTCACC TTAACCATTG CAAACCAGGG CACGCCGCTC
ACCTTCATGC ACCAGGAGTG TGAAGCCATT GTCCAGTCTA TCATTCATAT CCGGACCCGC
TGGGAACTGT CACAGCCCGA CTCTATCCCC CAACACACCA AGATTCGGCC AAAAGATGTC
CCTGGGACAC TGCTCAATAT CGCATTACTT AATTTAGGCA GTTCTGACCC GAGTTTACGG
TCAGCTGCCT ATAATCTTCT GTGTGCCTTA ACTTGTACCT TTAATTTAAA AATCGAGGGC
CAGTTACTAG AGACATCAGG TTTATGTATC CCTGCCAACA ACACCCTCTT TATTGTCTCT
ATTAGTAAGA CACTGGCAGC CAATGAGCCA CACCTCACGT TAGAATTTTT GGAAGAGTGT
ATTTCTGGAT TTAGCAAATC TAGTATTGAA TTGAAACACC TTTGTTTGGA ATACATGACT
CCATGGCTGT CAAATCTAGT TCGTTTTTGC AAGCATAATG ATGATGCCAA ACGACAAAGA
GTTACTGCTA TTCTTGACAA GCTGATAACA ATGACCATCA ATGAAAAACA GATGTACCCA
TCTATTCAAG CAAAAATATG GGGAAGCCTT GGGCAGATTA CAGATCTGCT TGATGTTGTA
CTAGACAGTT TCATCAAAAC CAGTGCAACA GGTGGCTTGG GATCAATAAA AGCTGAGGTG
ATGGCAGATA CTGCTGTAGC TTTGGCTTCT GGAAATGTGA AATTGGTTTC AAGCAAGGTT
ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACATGCTTAT CTCCAACTCC TACTTTAGAA
CAACATCTTA TGTGGGATGA TATTGCTATT TTAGCACGCT ACATGCTGAT GCTGTCCTTC
AACAATTCCC TTGATGTGGC AGCTCATCTT CCCTACCTCT TCCACGTTGT TACTTTCTTA
GTAGCCACAG GTCCGCTCTC CCTTAGAGCT TCCACACATG GACTGGTCAT TAATATCATT
CACTCTCTGT GTACTTGTTC ACAGCTTCAT TTTAGTGAAG AGACCAAGCA AGTTTTGAGA
CTCAGTCTGA CAGAGTTCTC ATTACCCAAA TTTTACTTGC TGTTTGGCAT TAGCAAAGTC
AAGTCAGCTG CTGTCATTGC CTTCCGTTCC AGTTACCGGG ACAGGTCATT CTCTCCTGGC
TCCTATGAGA GAGAGACTTT TGCTTTGACA TCCTTGGAAA CAGTCACAGA AGCTTTGTTG
GAGATCATGG AGGCATGCAT GAGAGATATT CCAACGTGCA AGTGGCTGGA CCAGTGGACA
GAACTAGCTC AAAGATTTGC ATTCCAATAT AATCCATCCC TGCAACCAAG AGCTCTTGTT
GTCTTTGGGT GTATTAGCAA ACGAGTGTCT CATGGGCAGA TAAAGCAGAT AATCCGTATT
CTTAGCAAGG CACTTGAGAG TTGCTTAAAA GGACCTGACA CTTACAACAG TCAAGTTCTG
ATAGAAGCTA CAGTAATAGC ACTAACCAAA TTACAGCCAC TTCTTAATAA GGACTCGCCT
CTGCACAAAG CCCTCTTTTG GGTAGCTGTG GCTGTGCTGC AGCTTGATGA GGTCAACTTG
TATTCAGCAG GTACCGCACT TCTTGAACAA AACCTGCATA CTTTAGATAG TCTCCGTATA
TTCAATGACA AGAGTCCAGA GGAAGTATTT ATGGCAATCC GGAATCCTCT GGAGTGGCAC
TGCAAGCAAA TGGATCATTT TGTTGGACTC AATTTCAACT CTAACTTTAA CTTTGCATTG
GTTGGACACC TTTTAAAAGG GTACAGGCAT CCTTCACCTG CTATTGTTGC AAGAACAGTC
AGAATTTTAC ATACACTACT AACTCTGGTT AACAAACACA GAAATTGTGA CAAATTTGAA
GTGAATACAC AGAGCGTGGC CTACTTAGCA GCTTTACTTA CAGTGTCTGA AGAAGTTCGA
AGTCGCTGCA GCCTAAAACA TAGAAAGTCA CTTCTTCTTA CTGATATTTC AATGGAAAAT
GTTCCTATGG ATACATATCC CATTCATCAT GGTGACCCTT CCTATAGGAC ACTAAAGGAG
ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT GAAGGATACC TTGCAGCCAC CTATCCAACT
GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA TCCATGAGCC TGGACATGGG GCAACCTTCT
CAGGCCAACA CTAAGAAGTT GCTTGGAACA AGGAAAAGTT TTGATCACTT GATATCAGAC
ACAAAGGCTC CTAAAAGGCA AGAAATGGAA TCAGGGATCA CAACACCCCC CAAAATGAGG
AGAGTAGCAG AAACTGATTA TGAAATGGAA ACTCAGAGGA TTTCCTCATC ACAACAGCAC
CCACATTTAC GTAAAGTTTC AGTGTCTGAA TCAAATGTTC TCTTGGATGA AGAAGTACTT
ACTGATCCGA AGATCCAGGC GCTGCTTCTT ACTGTTCTAG CTACACTGGT AAAATATACC
ACAGATGAGT TTGATCAACG AATTCTTTAT GAATACTTAG CAGAGGCCAG TGTTGTGTTT
CCCAAAGTCT TTCCTGTTGT GCATAATTTG TTGGACTCTA AGATCAACAC CCTGTTATCA
TTGTGCCAAG ATCCAAATTT GTTAAATCCA ATCCATGGAA TTGTGCAGAG TGTGGTGTAC
CATGAAGAAT CCCCACCACA ATACCAAACA TCTTACCTGC AAAGTTTTGG TTTTAATGGC
TTGTGGCGGT TTGCAGGACC GTTTTCAAAG CAAACACAAA TTCCAGACTA TGCTGAGCTT
ATTGTTAAGT TTCTTGATGC CTTGATTGAC ACGTACCTGC CTGGAATTGA TGAAGAAACC
AGTGAAGAAT CCCTCCTGAC TCCCACATCT CCTTACCCTC CTGCACTGCA GAGCCAGCTT
AGTATCACTG CCAACCTTAA CCTTTCTAAT TCCATGACCT CACTTGCAAC TTCCCAGCAT
TCCCCAGGAA TCGACAAGGA GAACGTTGAA CTCTCCCCTA CCACTGGCCA CTGTAACAGT
GGACGAACTC GCCACGGATC CGCAAGCCAA GTGCAGAAGC AAAGAAGCGC TGGCAGTTTC
AAACGTAATA GCATTAAGAA GATCGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006721987.1
CDS334..8883
Misc Feature(1)3922..5034(+)
Misc Feature(2)3955..5016(+)
Misc Feature(3)4069..4737(+)
Misc Feature(4)5104..5547(+)
Misc Feature(5)5173..5478(+)
Misc Feature(6)5377..5475(+)
Misc Feature(7)5545..5874(+)
Misc Feature(8)5545..5874(+)
Translation

Target ORF information:

RefSeq Version XM_006721924
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006721924

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
CGGAGGTTCA AAACTGGTCA AATCAATGGT GATTTGCTGA TATACCATGT CTTACTGACT
TTAAAGCCAT ATTATGCAAA GCCATATGAA ATTGTAGTGG ACCTTACCCA TACCGGGCCT
AGCAATCGCT TTAAAACAGA CTTTCTCTCT AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT
TACGACAACG TCTCCGCAGT CTATATCTAT AACTGTAACT CCTGGGTCAG GGAGTACACC
AAGTATCATG AGCGGCTGCT GACTGGCCTC AAAGGTAGCA AAAGGCTTGT TTTCATAGAC
TGTCCTGGGA AACTGGCTGA GCACATAGAG CATGAACAAC AGAAACTACC TGCTGCCACC
TTGGCTTTAG AAGAGGACCT GAAGGTATTC CACAATGCTC TCAAGCTAGC TCACAAAGAC
ACCAAAGTTT CTATTAAAGT TGGTTCTACT GCTGTCCAAG TAACTTCAGC AGAGCGAACA
AAAGTCCTAG GGCAATCAGT CTTTCTAAAT GACATTTATT ATGCTTCGGA AATTGAAGAA
ATCTGCCTAG TAGATGAGAA CCAGTTCACC TTAACCATTG CAAACCAGGG CACGCCGCTC
ACCTTCATGC ACCAGGAGTG TGAAGCCATT GTCCAGTCTA TCATTCATAT CCGGACCCGC
TGGGAACTGT CACAGCCCGA CTCTATCCCC CAACACACCA AGATTCGGCC AAAAGATGTC
CCTGGGACAC TGCTCAATAT CGCATTACTT AATTTAGGCA GTTCTGACCC GAGTTTACGG
TCAGCTGCCT ATAATCTTCT GTGTGCCTTA ACTTGTACCT TTAATTTAAA AATCGAGGGC
CAGTTACTAG AGACATCAGG TTTATGTATC CCTGCCAACA ACACCCTCTT TATTGTCTCT
ATTAGTAAGA CACTGGCAGC CAATGAGCCA CACCTCACGT TAGAATTTTT GGAAGAGTGT
ATTTCTGGAT TTAGCAAATC TAGTATTGAA TTGAAACACC TTTGTTTGGA ATACATGACT
CCATGGCTGT CAAATCTAGT TCGTTTTTGC AAGCATAATG ATGATGCCAA ACGACAAAGA
GTTACTGCTA TTCTTGACAA GCTGATAACA ATGACCATCA ATGAAAAACA GATGTACCCA
TCTATTCAAG CAAAAATATG GGGAAGCCTT GGGCAGATTA CAGATCTGCT TGATGTTGTA
CTAGACAGTT TCATCAAAAC CAGTGCAACA GGTGGCTTGG GATCAATAAA AGCTGAGGTG
ATGGCAGATA CTGCTGTAGC TTTGGCTTCT GGAAATGTGA AATTGGTTTC AAGCAAGGTT
ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACATGCTTAT CTCCAACTCC TACTTTAGAA
CAACATCTTA TGTGGGATGA TATTGCTATT TTAGCACGCT ACATGCTGAT GCTGTCCTTC
AACAATTCCC TTGATGTGGC AGCTCATCTT CCCTACCTCT TCCACGTTGT TACTTTCTTA
GTAGCCACAG GTCCGCTCTC CCTTAGAGCT TCCACACATG GACTGGTCAT TAATATCATT
CACTCTCTGT GTACTTGTTC ACAGCTTCAT TTTAGTGAAG AGACCAAGCA AGTTTTGAGA
CTCAGTCTGA CAGAGTTCTC ATTACCCAAA TTTTACTTGC TGTTTGGCAT TAGCAAAGTC
AAGTCAGCTG CTGTCATTGC CTTCCGTTCC AGTTACCGGG ACAGGTCATT CTCTCCTGGC
TCCTATGAGA GAGAGACTTT TGCTTTGACA TCCTTGGAAA CAGTCACAGA AGCTTTGTTG
GAGATCATGG AGGCATGCAT GAGAGATATT CCAACGTGCA AGTGGCTGGA CCAGTGGACA
GAACTAGCTC AAAGATTTGC ATTCCAATAT AATCCATCCC TGCAACCAAG AGCTCTTGTT
GTCTTTGGGT GTATTAGCAA ACGAGTGTCT CATGGGCAGA TAAAGCAGAT AATCCGTATT
CTTAGCAAGG CACTTGAGAG TTGCTTAAAA GGACCTGACA CTTACAACAG TCAAGTTCTG
ATAGAAGCTA CAGTAATAGC ACTAACCAAA TTACAGCCAC TTCTTAATAA GGACTCGCCT
CTGCACAAAG CCCTCTTTTG GGTAGCTGTG GCTGTGCTGC AGCTTGATGA GGTCAACTTG
TATTCAGCAG GTACCGCACT TCTTGAACAA AACCTGCATA CTTTAGATAG TCTCCGTATA
TTCAATGACA AGAGTCCAGA GGAAGTATTT ATGGCAATCC GGAATCCTCT GGAGTGGCAC
TGCAAGCAAA TGGATCATTT TGTTGGACTC AATTTCAACT CTAACTTTAA CTTTGCATTG
GTTGGACACC TTTTAAAAGG GTACAGGCAT CCTTCACCTG CTATTGTTGC AAGAACAGTC
AGAATTTTAC ATACACTACT AACTCTGGTT AACAAACACA GAAATTGTGA CAAATTTGAA
GTGAATACAC AGAGCGTGGC CTACTTAGCA GCTTTACTTA CAGTGTCTGA AGAAGTTCGA
AGTCGCTGCA GCCTAAAACA TAGAAAGTCA CTTCTTCTTA CTGATATTTC AATGGAAAAT
GTTCCTATGG ATACATATCC CATTCATCAT GGTGACCCTT CCTATAGGAC ACTAAAGGAG
ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT GAAGGATACC TTGCAGCCAC CTATCCAACT
GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA TCCATGAGCC TGGACATGGG GCAACCTTCT
CAGGCCAACA CTAAGAAGTT GCTTGGAACA AGGAAAAGTT TTGATCACTT GATATCAGAC
ACAAAGGCTC CTAAAAGGCA AGAAATGGAA TCAGGGATCA CAACACCCCC CAAAATGAGG
AGAGTAGCAG AAACTGATTA TGAAATGGAA ACTCAGAGGA TTTCCTCATC ACAACAGCAC
CCACATTTAC GTAAAGTTTC AGTGTCTGAA TCAAATGTTC TCTTGGATGA AGAAGTACTT
ACTGATCCGA AGATCCAGGC GCTGCTTCTT ACTGTTCTAG CTACACTGGT AAAATATACC
ACAGATGAGT TTGATCAACG AATTCTTTAT GAATACTTAG CAGAGGCCAG TGTTGTGTTT
CCCAAAGTCT TTCCTGTTGT GCATAATTTG TTGGACTCTA AGATCAACAC CCTGTTATCA
TTGTGCCAAG ATCCAAATTT GTTAAATCCA ATCCATGGAA TTGTGCAGAG TGTGGTGTAC
CATGAAGAAT CCCCACCACA ATACCAAACA TCTTACCTGC AAAGTTTTGG TTTTAATGGC
TTGTGGCGGT TTGCAGGACC GTTTTCAAAG CAAACACAAA TTCCAGACTA TGCTGAGCTT
ATTGTTAAGT TTCTTGATGC CTTGATTGAC ACGTACCTGC CTGGAATTGA TGAAGAAACC
AGTGAAGAAT CCCTCCTGAC TCCCACATCT CCTTACCCTC CTGCACTGCA GAGCCAGCTT
AGTATCACTG CCAACCTTAA CCTTTCTAAT TCCATGACCT CACTTGCAAC TTCCCAGCAT
TCCCCAGGAA TCGACAAGGA GAACGTTGAA CTCTCCCCTA CCACTGGCCA CTGTAACAGT
GGACGAACTC GCCACGGATC CGCAAGCCAA GTGCAGAAGC AAAGAAGCGC TGGCAGTTTC
AAACGTAATA GCATTAAGAA GATCGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50431
Clone ID Related Accession (Same CDS sequence) XM_006721925
Accession Version XM_006721925.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8541bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neurofibromin isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
GTGGTTAGCC AGCGTTTCCC TCAGAACAGC ATCGGTGCAG TAGGAAGTGC CATGTTCCTC
AGATTTATCA ATCCTGCCAT TGTCTCACCG TATGAAGCAG GGATTTTAGA TAAAAAGCCA
CCACCTAGAA TCGAAAGGGG CTTGAAGTTA ATGTCAAAGA TACTTCAGAG TATTGCCAAT
CATGTTCTCT TCACAAAAGA AGAACATATG CGGCCTTTCA ATGATTTTGT GAAAAGCAAC
TTTGATGCAG CACGCAGGTT TTTCCTTGAT ATAGCATCTG ATTGTCCTAC AAGTGATGCA
GTAAATCATA GTCTTTCCTT CATAAGTGAC GGCAATGTGC TTGCTTTACA TCGTCTACTC
TGGAACAATC AGGAGAAAAT TGGGCAGTAT CTTTCCAGCA ACAGGGATCA TAAAGCTGTT
GGAAGACGAC CTTTTGATAA GATGGCAACA CTTCTTGCAT ACCTGGGTCC TCCAGAGCAC
AAACCTGTGG CAGATACACA CTGGTCCAGC CTTAACCTTA CCAGTTCAAA GTTTGAGGAA
TTTATGACTA GGCATCAGGT ACATGAAAAA GAAGAATTCA AGGCTTTGAA AACGTTAAGT
ATTTTCTACC AAGCTGGGAC TTCCAAAGCT GGGAATCCTA TTTTTTATTA TGTTGCACGG
AGGTTCAAAA CTGGTCAAAT CAATGGTGAT TTGCTGATAT ACCATGTCTT ACTGACTTTA
AAGCCATATT ATGCAAAGCC ATATGAAATT GTAGTGGACC TTACCCATAC CGGGCCTAGC
AATCGCTTTA AAACAGACTT TCTCTCTAAG TGGTTTGTTG TTTTTCCTGG CTTTGCTTAC
GACAACGTCT CCGCAGTCTA TATCTATAAC TGTAACTCCT GGGTCAGGGA GTACACCAAG
TATCATGAGC GGCTGCTGAC TGGCCTCAAA GGTAGCAAAA GGCTTGTTTT CATAGACTGT
CCTGGGAAAC TGGCTGAGCA CATAGAGCAT GAACAACAGA AACTACCTGC TGCCACCTTG
GCTTTAGAAG AGGACCTGAA GGTATTCCAC AATGCTCTCA AGCTAGCTCA CAAAGACACC
AAAGTTTCTA TTAAAGTTGG TTCTACTGCT GTCCAAGTAA CTTCAGCAGA GCGAACAAAA
GTCCTAGGGC AATCAGTCTT TCTAAATGAC ATTTATTATG CTTCGGAAAT TGAAGAAATC
TGCCTAGTAG ATGAGAACCA GTTCACCTTA ACCATTGCAA ACCAGGGCAC GCCGCTCACC
TTCATGCACC AGGAGTGTGA AGCCATTGTC CAGTCTATCA TTCATATCCG GACCCGCTGG
GAACTGTCAC AGCCCGACTC TATCCCCCAA CACACCAAGA TTCGGCCAAA AGATGTCCCT
GGGACACTGC TCAATATCGC ATTACTTAAT TTAGGCAGTT CTGACCCGAG TTTACGGTCA
GCTGCCTATA ATCTTCTGTG TGCCTTAACT TGTACCTTTA ATTTAAAAAT CGAGGGCCAG
TTACTAGAGA CATCAGGTTT ATGTATCCCT GCCAACAACA CCCTCTTTAT TGTCTCTATT
AGTAAGACAC TGGCAGCCAA TGAGCCACAC CTCACGTTAG AATTTTTGGA AGAGTGTATT
TCTGGATTTA GCAAATCTAG TATTGAATTG AAACACCTTT GTTTGGAATA CATGACTCCA
TGGCTGTCAA ATCTAGTTCG TTTTTGCAAG CATAATGATG ATGCCAAACG ACAAAGAGTT
ACTGCTATTC TTGACAAGCT GATAACAATG ACCATCAATG AAAAACAGAT GTACCCATCT
ATTCAAGCAA AAATATGGGG AAGCCTTGGG CAGATTACAG ATCTGCTTGA TGTTGTACTA
GACAGTTTCA TCAAAACCAG TGCAACAGGT GGCTTGGGAT CAATAAAAGC TGAGGTGATG
GCAGATACTG CTGTAGCTTT GGCTTCTGGA AATGTGAAAT TGGTTTCAAG CAAGGTTATT
GGAAGGATGT GCAAAATAAT TGACAAGACA TGCTTATCTC CAACTCCTAC TTTAGAACAA
CATCTTATGT GGGATGATAT TGCTATTTTA GCACGCTACA TGCTGATGCT GTCCTTCAAC
AATTCCCTTG ATGTGGCAGC TCATCTTCCC TACCTCTTCC ACGTTGTTAC TTTCTTAGTA
GCCACAGGTC CGCTCTCCCT TAGAGCTTCC ACACATGGAC TGGTCATTAA TATCATTCAC
TCTCTGTGTA CTTGTTCACA GCTTCATTTT AGTGAAGAGA CCAAGCAAGT TTTGAGACTC
AGTCTGACAG AGTTCTCATT ACCCAAATTT TACTTGCTGT TTGGCATTAG CAAAGTCAAG
TCAGCTGCTG TCATTGCCTT CCGTTCCAGT TACCGGGACA GGTCATTCTC TCCTGGCTCC
TATGAGAGAG AGACTTTTGC TTTGACATCC TTGGAAACAG TCACAGAAGC TTTGTTGGAG
ATCATGGAGG CATGCATGAG AGATATTCCA ACGTGCAAGT GGCTGGACCA GTGGACAGAA
CTAGCTCAAA GATTTGCATT CCAATATAAT CCATCCCTGC AACCAAGAGC TCTTGTTGTC
TTTGGGTGTA TTAGCAAACG AGTGTCTCAT GGGCAGATAA AGCAGATAAT CCGTATTCTT
AGCAAGGCAC TTGAGAGTTG CTTAAAAGGA CCTGACACTT ACAACAGTCA AGTTCTGATA
GAAGCTACAG TAATAGCACT AACCAAATTA CAGCCACTTC TTAATAAGGA CTCGCCTCTG
CACAAAGCCC TCTTTTGGGT AGCTGTGGCT GTGCTGCAGC TTGATGAGGT CAACTTGTAT
TCAGCAGGTA CCGCACTTCT TGAACAAAAC CTGCATACTT TAGATAGTCT CCGTATATTC
AATGACAAGA GTCCAGAGGA AGTATTTATG GCAATCCGGA ATCCTCTGGA GTGGCACTGC
AAGCAAATGG ATCATTTTGT TGGACTCAAT TTCAACTCTA ACTTTAACTT TGCATTGGTT
GGACACCTTT TAAAAGGGTA CAGGCATCCT TCACCTGCTA TTGTTGCAAG AACAGTCAGA
ATTTTACATA CACTACTAAC TCTGGTTAAC AAACACAGAA ATTGTGACAA ATTTGAAGTG
AATACACAGA GCGTGGCCTA CTTAGCAGCT TTACTTACAG TGTCTGAAGA AGTTCGAAGT
CGCTGCAGCC TAAAACATAG AAAGTCACTT CTTCTTACTG ATATTTCAAT GGAAAATGTT
CCTATGGATA CATATCCCAT TCATCATGGT GACCCTTCCT ATAGGACACT AAAGGAGACT
CAGCCATGGT CCTCTCCCAA AGGTTCTGAA GGATACCTTG CAGCCACCTA TCCAACTGTC
GGCCAGACCA GTCCCCGAGC CAGGAAATCC ATGAGCCTGG ACATGGGGCA ACCTTCTCAG
GCCAACACTA AGAAGTTGCT TGGAACAAGG AAAAGTTTTG ATCACTTGAT ATCAGACACA
AAGGCTCCTA AAAGGCAAGA AATGGAATCA GGGATCACAA CACCCCCCAA AATGAGGAGA
GTAGCAGAAA CTGATTATGA AATGGAAACT CAGAGGATTT CCTCATCACA ACAGCACCCA
CATTTACGTA AAGTTTCAGT GTCTGAATCA AATGTTCTCT TGGATGAAGA AGTACTTACT
GATCCGAAGA TCCAGGCGCT GCTTCTTACT GTTCTAGCTA CACTGGTAAA ATATACCACA
GATGAGTTTG ATCAACGAAT TCTTTATGAA TACTTAGCAG AGGCCAGTGT TGTGTTTCCC
AAAGTCTTTC CTGTTGTGCA TAATTTGTTG GACTCTAAGA TCAACACCCT GTTATCATTG
TGCCAAGATC CAAATTTGTT AAATCCAATC CATGGAATTG TGCAGAGTGT GGTGTACCAT
GAAGAATCCC CACCACAATA CCAAACATCT TACCTGCAAA GTTTTGGTTT TAATGGCTTG
TGGCGGTTTG CAGGACCGTT TTCAAAGCAA ACACAAATTC CAGACTATGC TGAGCTTATT
GTTAAGTTTC TTGATGCCTT GATTGACACG TACCTGCCTG GAATTGATGA AGAAACCAGT
GAAGAATCCC TCCTGACTCC CACATCTCCT TACCCTCCTG CACTGCAGAG CCAGCTTAGT
ATCACTGCCA ACCTTAACCT TTCTAATTCC ATGACCTCAC TTGCAACTTC CCAGCATTCC
CCAGCTTCTC TGCCTTGCTC TAAATCAGCA GTTTTCATGC AGCTGTTCCC TCATCAAGGA
ATCGACAAGG AGAACGTTGA ACTCTCCCCT ACCACTGGCC ACTGTAACAG TGGACGAACT
CGCCACGGAT CCGCAAGCCA AGTGCAGAAG CAAAGAAGCG CTGGCAGTTT CAAACGTAAT
AGCATTAAGA AGATCGTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006721988.1
CDS334..8874
Misc Feature(1)3922..4971(+)
Misc Feature(2)3955..4953(+)
Misc Feature(3)4069..4674(+)
Misc Feature(4)5041..5484(+)
Misc Feature(5)5110..5415(+)
Misc Feature(6)5314..5412(+)
Misc Feature(7)5482..5811(+)
Misc Feature(8)5482..5811(+)
Translation

Target ORF information:

RefSeq Version XM_006721925
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006721925

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
GTGGTTAGCC AGCGTTTCCC TCAGAACAGC ATCGGTGCAG TAGGAAGTGC CATGTTCCTC
AGATTTATCA ATCCTGCCAT TGTCTCACCG TATGAAGCAG GGATTTTAGA TAAAAAGCCA
CCACCTAGAA TCGAAAGGGG CTTGAAGTTA ATGTCAAAGA TACTTCAGAG TATTGCCAAT
CATGTTCTCT TCACAAAAGA AGAACATATG CGGCCTTTCA ATGATTTTGT GAAAAGCAAC
TTTGATGCAG CACGCAGGTT TTTCCTTGAT ATAGCATCTG ATTGTCCTAC AAGTGATGCA
GTAAATCATA GTCTTTCCTT CATAAGTGAC GGCAATGTGC TTGCTTTACA TCGTCTACTC
TGGAACAATC AGGAGAAAAT TGGGCAGTAT CTTTCCAGCA ACAGGGATCA TAAAGCTGTT
GGAAGACGAC CTTTTGATAA GATGGCAACA CTTCTTGCAT ACCTGGGTCC TCCAGAGCAC
AAACCTGTGG CAGATACACA CTGGTCCAGC CTTAACCTTA CCAGTTCAAA GTTTGAGGAA
TTTATGACTA GGCATCAGGT ACATGAAAAA GAAGAATTCA AGGCTTTGAA AACGTTAAGT
ATTTTCTACC AAGCTGGGAC TTCCAAAGCT GGGAATCCTA TTTTTTATTA TGTTGCACGG
AGGTTCAAAA CTGGTCAAAT CAATGGTGAT TTGCTGATAT ACCATGTCTT ACTGACTTTA
AAGCCATATT ATGCAAAGCC ATATGAAATT GTAGTGGACC TTACCCATAC CGGGCCTAGC
AATCGCTTTA AAACAGACTT TCTCTCTAAG TGGTTTGTTG TTTTTCCTGG CTTTGCTTAC
GACAACGTCT CCGCAGTCTA TATCTATAAC TGTAACTCCT GGGTCAGGGA GTACACCAAG
TATCATGAGC GGCTGCTGAC TGGCCTCAAA GGTAGCAAAA GGCTTGTTTT CATAGACTGT
CCTGGGAAAC TGGCTGAGCA CATAGAGCAT GAACAACAGA AACTACCTGC TGCCACCTTG
GCTTTAGAAG AGGACCTGAA GGTATTCCAC AATGCTCTCA AGCTAGCTCA CAAAGACACC
AAAGTTTCTA TTAAAGTTGG TTCTACTGCT GTCCAAGTAA CTTCAGCAGA GCGAACAAAA
GTCCTAGGGC AATCAGTCTT TCTAAATGAC ATTTATTATG CTTCGGAAAT TGAAGAAATC
TGCCTAGTAG ATGAGAACCA GTTCACCTTA ACCATTGCAA ACCAGGGCAC GCCGCTCACC
TTCATGCACC AGGAGTGTGA AGCCATTGTC CAGTCTATCA TTCATATCCG GACCCGCTGG
GAACTGTCAC AGCCCGACTC TATCCCCCAA CACACCAAGA TTCGGCCAAA AGATGTCCCT
GGGACACTGC TCAATATCGC ATTACTTAAT TTAGGCAGTT CTGACCCGAG TTTACGGTCA
GCTGCCTATA ATCTTCTGTG TGCCTTAACT TGTACCTTTA ATTTAAAAAT CGAGGGCCAG
TTACTAGAGA CATCAGGTTT ATGTATCCCT GCCAACAACA CCCTCTTTAT TGTCTCTATT
AGTAAGACAC TGGCAGCCAA TGAGCCACAC CTCACGTTAG AATTTTTGGA AGAGTGTATT
TCTGGATTTA GCAAATCTAG TATTGAATTG AAACACCTTT GTTTGGAATA CATGACTCCA
TGGCTGTCAA ATCTAGTTCG TTTTTGCAAG CATAATGATG ATGCCAAACG ACAAAGAGTT
ACTGCTATTC TTGACAAGCT GATAACAATG ACCATCAATG AAAAACAGAT GTACCCATCT
ATTCAAGCAA AAATATGGGG AAGCCTTGGG CAGATTACAG ATCTGCTTGA TGTTGTACTA
GACAGTTTCA TCAAAACCAG TGCAACAGGT GGCTTGGGAT CAATAAAAGC TGAGGTGATG
GCAGATACTG CTGTAGCTTT GGCTTCTGGA AATGTGAAAT TGGTTTCAAG CAAGGTTATT
GGAAGGATGT GCAAAATAAT TGACAAGACA TGCTTATCTC CAACTCCTAC TTTAGAACAA
CATCTTATGT GGGATGATAT TGCTATTTTA GCACGCTACA TGCTGATGCT GTCCTTCAAC
AATTCCCTTG ATGTGGCAGC TCATCTTCCC TACCTCTTCC ACGTTGTTAC TTTCTTAGTA
GCCACAGGTC CGCTCTCCCT TAGAGCTTCC ACACATGGAC TGGTCATTAA TATCATTCAC
TCTCTGTGTA CTTGTTCACA GCTTCATTTT AGTGAAGAGA CCAAGCAAGT TTTGAGACTC
AGTCTGACAG AGTTCTCATT ACCCAAATTT TACTTGCTGT TTGGCATTAG CAAAGTCAAG
TCAGCTGCTG TCATTGCCTT CCGTTCCAGT TACCGGGACA GGTCATTCTC TCCTGGCTCC
TATGAGAGAG AGACTTTTGC TTTGACATCC TTGGAAACAG TCACAGAAGC TTTGTTGGAG
ATCATGGAGG CATGCATGAG AGATATTCCA ACGTGCAAGT GGCTGGACCA GTGGACAGAA
CTAGCTCAAA GATTTGCATT CCAATATAAT CCATCCCTGC AACCAAGAGC TCTTGTTGTC
TTTGGGTGTA TTAGCAAACG AGTGTCTCAT GGGCAGATAA AGCAGATAAT CCGTATTCTT
AGCAAGGCAC TTGAGAGTTG CTTAAAAGGA CCTGACACTT ACAACAGTCA AGTTCTGATA
GAAGCTACAG TAATAGCACT AACCAAATTA CAGCCACTTC TTAATAAGGA CTCGCCTCTG
CACAAAGCCC TCTTTTGGGT AGCTGTGGCT GTGCTGCAGC TTGATGAGGT CAACTTGTAT
TCAGCAGGTA CCGCACTTCT TGAACAAAAC CTGCATACTT TAGATAGTCT CCGTATATTC
AATGACAAGA GTCCAGAGGA AGTATTTATG GCAATCCGGA ATCCTCTGGA GTGGCACTGC
AAGCAAATGG ATCATTTTGT TGGACTCAAT TTCAACTCTA ACTTTAACTT TGCATTGGTT
GGACACCTTT TAAAAGGGTA CAGGCATCCT TCACCTGCTA TTGTTGCAAG AACAGTCAGA
ATTTTACATA CACTACTAAC TCTGGTTAAC AAACACAGAA ATTGTGACAA ATTTGAAGTG
AATACACAGA GCGTGGCCTA CTTAGCAGCT TTACTTACAG TGTCTGAAGA AGTTCGAAGT
CGCTGCAGCC TAAAACATAG AAAGTCACTT CTTCTTACTG ATATTTCAAT GGAAAATGTT
CCTATGGATA CATATCCCAT TCATCATGGT GACCCTTCCT ATAGGACACT AAAGGAGACT
CAGCCATGGT CCTCTCCCAA AGGTTCTGAA GGATACCTTG CAGCCACCTA TCCAACTGTC
GGCCAGACCA GTCCCCGAGC CAGGAAATCC ATGAGCCTGG ACATGGGGCA ACCTTCTCAG
GCCAACACTA AGAAGTTGCT TGGAACAAGG AAAAGTTTTG ATCACTTGAT ATCAGACACA
AAGGCTCCTA AAAGGCAAGA AATGGAATCA GGGATCACAA CACCCCCCAA AATGAGGAGA
GTAGCAGAAA CTGATTATGA AATGGAAACT CAGAGGATTT CCTCATCACA ACAGCACCCA
CATTTACGTA AAGTTTCAGT GTCTGAATCA AATGTTCTCT TGGATGAAGA AGTACTTACT
GATCCGAAGA TCCAGGCGCT GCTTCTTACT GTTCTAGCTA CACTGGTAAA ATATACCACA
GATGAGTTTG ATCAACGAAT TCTTTATGAA TACTTAGCAG AGGCCAGTGT TGTGTTTCCC
AAAGTCTTTC CTGTTGTGCA TAATTTGTTG GACTCTAAGA TCAACACCCT GTTATCATTG
TGCCAAGATC CAAATTTGTT AAATCCAATC CATGGAATTG TGCAGAGTGT GGTGTACCAT
GAAGAATCCC CACCACAATA CCAAACATCT TACCTGCAAA GTTTTGGTTT TAATGGCTTG
TGGCGGTTTG CAGGACCGTT TTCAAAGCAA ACACAAATTC CAGACTATGC TGAGCTTATT
GTTAAGTTTC TTGATGCCTT GATTGACACG TACCTGCCTG GAATTGATGA AGAAACCAGT
GAAGAATCCC TCCTGACTCC CACATCTCCT TACCCTCCTG CACTGCAGAG CCAGCTTAGT
ATCACTGCCA ACCTTAACCT TTCTAATTCC ATGACCTCAC TTGCAACTTC CCAGCATTCC
CCAGCTTCTC TGCCTTGCTC TAAATCAGCA GTTTTCATGC AGCTGTTCCC TCATCAAGGA
ATCGACAAGG AGAACGTTGA ACTCTCCCCT ACCACTGGCC ACTGTAACAG TGGACGAACT
CGCCACGGAT CCGCAAGCCA AGTGCAGAAG CAAAGAAGCG CTGGCAGTTT CAAACGTAAT
AGCATTAAGA AGATCGTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50432
Clone ID Related Accession (Same CDS sequence) XM_005257984
Accession Version XM_005257984.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8511bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neurofibromin isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GCTCACAAAT GCTTTTTTAC
ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTG
CGGGAAATAT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
TCCTGTCACT TTCTCCTTTT TTACGGGGTA GGATGTGATA TTCCTTCTAG TGGAAATACC
AGTCAAATGT CCATGGATCA TGAAGAATTA CTACGTACTC CTGGAGCCTC TCTCCGGAAG
GGAAAAGGGA ACTCCTCTAT GGATAGTGCA GCAGGATGCA GCGGAACCCC CCCGATTTGC
CGACAAGCCC AGACCAAACT AGAAGTGGCC CTGTACATGT TTCTGTGGAA CCCTGACACT
GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC CGCCACCTCT GTGAGGAAGC AGATATCCGG
TGTGGGGTGG ATGAAGTGTC AGTGCATAAC CTCTTGCCCA ACTATAACAC ATTCATGGAG
TTTGCCTCTG TCAGCAATAT GATGTCAACA GGAAGAGCAG CACTTCAGAA AAGAGTGATG
GCACTGCTGA GGCGCATTGA GCATCCCACT GCAGGAAACA CTGAGGCTTG GGAAGATACA
CATGCAAAAT GGGAACAAGC AACAAAGCTA ATCCTTAACT ATCCAAAAGC CAAAATGGAA
GATGGCCAGG CTGCTGAAAG CCTTCACAAG ACCATTGTTA AGAGGCGAAT GTCCCATGTG
AGTGGAGGAG GATCCATAGA TTTGTCTGAC ACAGACTCCC TACAGGAATG GATCAACATG
ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG
GCAACCTATA GCCCACCCAT GGGTCCAGTC AGTGAACGTA AGGGTTCTAT GATTTCAGTG
ATGTCTTCAG AGGGAAACGC AGATACACCT GTCAGCAAAT TTATGGATCG GCTGTTGTCC
TTAATGGTGT GTAACCATGA GAAAGTGGGA CTTCAAATAC GGACCAATGT TAAGGATCTG
GTGGGTCTAG AATTGAGTCC TGCTCTGTAT CCAATGCTAT TTAACAAATT GAAGAATACC
ATCAGCAAGT TTTTTGACTC CCAAGGACAG GTTTTATTGA CTGATACCAA TACTCAATTT
GTAGAACAAA CCATAGCTAT AATGAAGAAC TTGCTAGATA ATCATACTGA AGGCAGCTCT
GAACATCTAG GGCAAGCTAG CATTGAAACA ATGATGTTAA ATCTGGTCAG GTATGTTCGT
GTGCTTGGGA ATATGGTCCA TGCAATTCAA ATAAAAACGA AACTGTGTCA ATTAGTTGAA
GTAATGATGG CAAGGAGAGA TGACCTCTCA TTTTGCCAAG AGATGAAATT TAGGAATAAG
ATGGTAGAAT ACCTGACAGA CTGGGTTATG GGAACATCAA ACCAAGCAGC AGATGATGAT
GTAAAATGTC TTACAAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTAGT TTCACTTCTA
GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA GATGGTGTGG AATTGATGGA AGCCAAATCA
CAGTTATTTC TTAAATACTT CACATTATTT ATGAACCTTT TGAATGACTG CAGTGAAGTT
GAAGATGAAA GTGCGCAAAC AGGTGGCAGG AAACGTGGCA TGTCTCGGAG GCTGGCATCA
CTGAGGCACT GTACGGTCCT TGCAATGTCA AACTTACTCA ATGCCAACGT AGACAGTGGT
CTCATGCACT CCATAGGCTT AGGTTACCAC AAGGATCTCC AGACAAGAGC TACATTTATG
GAAGTTCTGA CAAAAATCCT TCAACAAGGC ACAGAATTTG ACACACTTGC AGAAACAGTA
TTGGCTGATC GGTTTGAGAG ATTGGTGGAA CTGGTCACAA TGATGGGTGA TCAAGGAGAA
CTCCCTATAG CGATGGCTCT GGCCAATGTG GTTCCTTGTT CTCAGTGGGA TGAACTAGCT
CGAGTTCTGG TTACTCTGTT TGATTCTCGG CATTTACTCT ACCAACTGCT CTGGAACATG
TTTTCTAAAG AAGTAGAATT GGCAGACTCC ATGCAGACTC TCTTCCGAGG CAACAGCTTG
GCCAGTAAAA TAATGACATT CTGTTTCAAG GTATATGGTG CTACCTATCT ACAAAAACTC
CTGGATCCTT TATTACGAAT TGTGATCACA TCCTCTGATT GGCAACATGT TAGCTTTGAA
GTGGATCCTA CCAGGTTAGA ACCATCAGAG AGCCTTGAGG AAAACCAGCG GAACCTCCTT
CAGATGACTG AAAAGTTCTT CCATGCCATC ATCAGTTCCT CCTCAGAATT CCCCCCTCAA
CTTCGAAGTG TGTGCCACTG TTTATACCAG GTGGTTAGCC AGCGTTTCCC TCAGAACAGC
ATCGGTGCAG TAGGAAGTGC CATGTTCCTC AGATTTATCA ATCCTGCCAT TGTCTCACCG
TATGAAGCAG GGATTTTAGA TAAAAAGCCA CCACCTAGAA TCGAAAGGGG CTTGAAGTTA
ATGTCAAAGA TACTTCAGAG TATTGCCAAT CATGTTCTCT TCACAAAAGA AGAACATATG
CGGCCTTTCA ATGATTTTGT GAAAAGCAAC TTTGATGCAG CACGCAGGTT TTTCCTTGAT
ATAGCATCTG ATTGTCCTAC AAGTGATGCA GTAAATCATA GTCTTTCCTT CATAAGTGAC
GGCAATGTGC TTGCTTTACA TCGTCTACTC TGGAACAATC AGGAGAAAAT TGGGCAGTAT
CTTTCCAGCA ACAGGGATCA TAAAGCTGTT GGAAGACGAC CTTTTGATAA GATGGCAACA
CTTCTTGCAT ACCTGGGTCC TCCAGAGCAC AAACCTGTGG CAGATACACA CTGGTCCAGC
CTTAACCTTA CCAGTTCAAA GTTTGAGGAA TTTATGACTA GGCATCAGGT ACATGAAAAA
GAAGAATTCA AGGCTTTGAA AACGTTAAGT ATTTTCTACC AAGCTGGGAC TTCCAAAGCT
GGGAATCCTA TTTTTTATTA TGTTGCACGG AGGTTCAAAA CTGGTCAAAT CAATGGTGAT
TTGCTGATAT ACCATGTCTT ACTGACTTTA AAGCCATATT ATGCAAAGCC ATATGAAATT
GTAGTGGACC TTACCCATAC CGGGCCTAGC AATCGCTTTA AAACAGACTT TCTCTCTAAG
TGGTTTGTTG TTTTTCCTGG CTTTGCTTAC GACAACGTCT CCGCAGTCTA TATCTATAAC
TGTAACTCCT GGGTCAGGGA GTACACCAAG TATCATGAGC GGCTGCTGAC TGGCCTCAAA
GGTAGCAAAA GGCTTGTTTT CATAGACTGT CCTGGGAAAC TGGCTGAGCA CATAGAGCAT
GAACAACAGA AACTACCTGC TGCCACCTTG GCTTTAGAAG AGGACCTGAA GGTATTCCAC
AATGCTCTCA AGCTAGCTCA CAAAGACACC AAAGTTTCTA TTAAAGTTGG TTCTACTGCT
GTCCAAGTAA CTTCAGCAGA GCGAACAAAA GTCCTAGGGC AATCAGTCTT TCTAAATGAC
ATTTATTATG CTTCGGAAAT TGAAGAAATC TGCCTAGTAG ATGAGAACCA GTTCACCTTA
ACCATTGCAA ACCAGGGCAC GCCGCTCACC TTCATGCACC AGGAGTGTGA AGCCATTGTC
CAGTCTATCA TTCATATCCG GACCCGCTGG GAACTGTCAC AGCCCGACTC TATCCCCCAA
CACACCAAGA TTCGGCCAAA AGATGTCCCT GGGACACTGC TCAATATCGC ATTACTTAAT
TTAGGCAGTT CTGACCCGAG TTTACGGTCA GCTGCCTATA ATCTTCTGTG TGCCTTAACT
TGTACCTTTA ATTTAAAAAT CGAGGGCCAG TTACTAGAGA CATCAGGTTT ATGTATCCCT
GCCAACAACA CCCTCTTTAT TGTCTCTATT AGTAAGACAC TGGCAGCCAA TGAGCCACAC
CTCACGTTAG AATTTTTGGA AGAGTGTATT TCTGGATTTA GCAAATCTAG TATTGAATTG
AAACACCTTT GTTTGGAATA CATGACTCCA TGGCTGTCAA ATCTAGTTCG TTTTTGCAAG
CATAATGATG ATGCCAAACG ACAAAGAGTT ACTGCTATTC TTGACAAGCT GATAACAATG
ACCATCAATG AAAAACAGAT GTACCCATCT ATTCAAGCAA AAATATGGGG AAGCCTTGGG
CAGATTACAG ATCTGCTTGA TGTTGTACTA GACAGTTTCA TCAAAACCAG TGCAACAGGT
GGCTTGGGAT CAATAAAAGC TGAGGTGATG GCAGATACTG CTGTAGCTTT GGCTTCTGGA
AATGTGAAAT TGGTTTCAAG CAAGGTTATT GGAAGGATGT GCAAAATAAT TGACAAGACA
TGCTTATCTC CAACTCCTAC TTTAGAACAA CATCTTATGT GGGATGATAT TGCTATTTTA
GCACGCTACA TGCTGATGCT GTCCTTCAAC AATTCCCTTG ATGTGGCAGC TCATCTTCCC
TACCTCTTCC ACGTTGTTAC TTTCTTAGTA GCCACAGGTC CGCTCTCCCT TAGAGCTTCC
ACACATGGAC TGGTCATTAA TATCATTCAC TCTCTGTGTA CTTGTTCACA GCTTCATTTT
AGTGAAGAGA CCAAGCAAGT TTTGAGACTC AGTCTGACAG AGTTCTCATT ACCCAAATTT
TACTTGCTGT TTGGCATTAG CAAAGTCAAG TCAGCTGCTG TCATTGCCTT CCGTTCCAGT
TACCGGGACA GGTCATTCTC TCCTGGCTCC TATGAGAGAG AGACTTTTGC TTTGACATCC
TTGGAAACAG TCACAGAAGC TTTGTTGGAG ATCATGGAGG CATGCATGAG AGATATTCCA
ACGTGCAAGT GGCTGGACCA GTGGACAGAA CTAGCTCAAA GATTTGCATT CCAATATAAT
CCATCCCTGC AACCAAGAGC TCTTGTTGTC TTTGGGTGTA TTAGCAAACG AGTGTCTCAT
GGGCAGATAA AGCAGATAAT CCGTATTCTT AGCAAGGCAC TTGAGAGTTG CTTAAAAGGA
CCTGACACTT ACAACAGTCA AGTTCTGATA GAAGCTACAG TAATAGCACT AACCAAATTA
CAGCCACTTC TTAATAAGGA CTCGCCTCTG CACAAAGCCC TCTTTTGGGT AGCTGTGGCT
GTGCTGCAGC TTGATGAGGT CAACTTGTAT TCAGCAGGTA CCGCACTTCT TGAACAAAAC
CTGCATACTT TAGATAGTCT CCGTATATTC AATGACAAGA GTCCAGAGGA AGTATTTATG
GCAATCCGGA ATCCTCTGGA GTGGCACTGC AAGCAAATGG ATCATTTTGT TGGACTCAAT
TTCAACTCTA ACTTTAACTT TGCATTGGTT GGACACCTTT TAAAAGGGTA CAGGCATCCT
TCACCTGCTA TTGTTGCAAG AACAGTCAGA ATTTTACATA CACTACTAAC TCTGGTTAAC
AAACACAGAA ATTGTGACAA ATTTGAAGTG AATACACAGA GCGTGGCCTA CTTAGCAGCT
TTACTTACAG TGTCTGAAGA AGTTCGAAGT CGCTGCAGCC TAAAACATAG AAAGTCACTT
CTTCTTACTG ATATTTCAAT GGAAAATGTT CCTATGGATA CATATCCCAT TCATCATGGT
GACCCTTCCT ATAGGACACT AAAGGAGACT CAGCCATGGT CCTCTCCCAA AGGTTCTGAA
GGATACCTTG CAGCCACCTA TCCAACTGTC GGCCAGACCA GTCCCCGAGC CAGGAAATCC
ATGAGCCTGG ACATGGGGCA ACCTTCTCAG GCCAACACTA AGAAGTTGCT TGGAACAAGG
AAAAGTTTTG ATCACTTGAT ATCAGACACA AAGGCTCCTA AAAGGCAAGA AATGGAATCA
GGGATCACAA CACCCCCCAA AATGAGGAGA GTAGCAGAAA CTGATTATGA AATGGAAACT
CAGAGGATTT CCTCATCACA ACAGCACCCA CATTTACGTA AAGTTTCAGT GTCTGAATCA
AATGTTCTCT TGGATGAAGA AGTACTTACT GATCCGAAGA TCCAGGCGCT GCTTCTTACT
GTTCTAGCTA CACTGGTAAA ATATACCACA GATGAGTTTG ATCAACGAAT TCTTTATGAA
TACTTAGCAG AGGCCAGTGT TGTGTTTCCC AAAGTCTTTC CTGTTGTGCA TAATTTGTTG
GACTCTAAGA TCAACACCCT GTTATCATTG TGCCAAGATC CAAATTTGTT AAATCCAATC
CATGGAATTG TGCAGAGTGT GGTGTACCAT GAAGAATCCC CACCACAATA CCAAACATCT
TACCTGCAAA GTTTTGGTTT TAATGGCTTG TGGCGGTTTG CAGGACCGTT TTCAAAGCAA
ACACAAATTC CAGACTATGC TGAGCTTATT GTTAAGTTTC TTGATGCCTT GATTGACACG
TACCTGCCTG GAATTGATGA AGAAACCAGT GAAGAATCCC TCCTGACTCC CACATCTCCT
TACCCTCCTG CACTGCAGAG CCAGCTTAGT ATCACTGCCA ACCTTAACCT TTCTAATTCC
ATGACCTCAC TTGCAACTTC CCAGCATTCC CCAGCTTCTC TGCCTTGCTC TAAATCAGCA
GTTTTCATGC AGCTGTTCCC TCATCAAGGA ATCGACAAGG AGAACGTTGA ACTCTCCCCT
ACCACTGGCC ACTGTAACAG TGGACGAACT CGCCACGGAT CCGCAAGCCA AGTGCAGAAG
CAAAGAAGCG CTGGCAGTTT CAAACGTAAT AGCATTAAGA AGATCGTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005258041.1
CDS334..8844
Misc Feature(1)3892..4941(+)
Misc Feature(2)3925..4923(+)
Misc Feature(3)4039..4644(+)
Misc Feature(4)5011..5454(+)
Misc Feature(5)5080..5385(+)
Misc Feature(6)5284..5382(+)
Misc Feature(7)5452..5781(+)
Misc Feature(8)5452..5781(+)
Translation

Target ORF information:

RefSeq Version XM_005257984
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257984

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GCTCACAAAT GCTTTTTTAC
ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTG
CGGGAAATAT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
TCCTGTCACT TTCTCCTTTT TTACGGGGTA GGATGTGATA TTCCTTCTAG TGGAAATACC
AGTCAAATGT CCATGGATCA TGAAGAATTA CTACGTACTC CTGGAGCCTC TCTCCGGAAG
GGAAAAGGGA ACTCCTCTAT GGATAGTGCA GCAGGATGCA GCGGAACCCC CCCGATTTGC
CGACAAGCCC AGACCAAACT AGAAGTGGCC CTGTACATGT TTCTGTGGAA CCCTGACACT
GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC CGCCACCTCT GTGAGGAAGC AGATATCCGG
TGTGGGGTGG ATGAAGTGTC AGTGCATAAC CTCTTGCCCA ACTATAACAC ATTCATGGAG
TTTGCCTCTG TCAGCAATAT GATGTCAACA GGAAGAGCAG CACTTCAGAA AAGAGTGATG
GCACTGCTGA GGCGCATTGA GCATCCCACT GCAGGAAACA CTGAGGCTTG GGAAGATACA
CATGCAAAAT GGGAACAAGC AACAAAGCTA ATCCTTAACT ATCCAAAAGC CAAAATGGAA
GATGGCCAGG CTGCTGAAAG CCTTCACAAG ACCATTGTTA AGAGGCGAAT GTCCCATGTG
AGTGGAGGAG GATCCATAGA TTTGTCTGAC ACAGACTCCC TACAGGAATG GATCAACATG
ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG
GCAACCTATA GCCCACCCAT GGGTCCAGTC AGTGAACGTA AGGGTTCTAT GATTTCAGTG
ATGTCTTCAG AGGGAAACGC AGATACACCT GTCAGCAAAT TTATGGATCG GCTGTTGTCC
TTAATGGTGT GTAACCATGA GAAAGTGGGA CTTCAAATAC GGACCAATGT TAAGGATCTG
GTGGGTCTAG AATTGAGTCC TGCTCTGTAT CCAATGCTAT TTAACAAATT GAAGAATACC
ATCAGCAAGT TTTTTGACTC CCAAGGACAG GTTTTATTGA CTGATACCAA TACTCAATTT
GTAGAACAAA CCATAGCTAT AATGAAGAAC TTGCTAGATA ATCATACTGA AGGCAGCTCT
GAACATCTAG GGCAAGCTAG CATTGAAACA ATGATGTTAA ATCTGGTCAG GTATGTTCGT
GTGCTTGGGA ATATGGTCCA TGCAATTCAA ATAAAAACGA AACTGTGTCA ATTAGTTGAA
GTAATGATGG CAAGGAGAGA TGACCTCTCA TTTTGCCAAG AGATGAAATT TAGGAATAAG
ATGGTAGAAT ACCTGACAGA CTGGGTTATG GGAACATCAA ACCAAGCAGC AGATGATGAT
GTAAAATGTC TTACAAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTAGT TTCACTTCTA
GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA GATGGTGTGG AATTGATGGA AGCCAAATCA
CAGTTATTTC TTAAATACTT CACATTATTT ATGAACCTTT TGAATGACTG CAGTGAAGTT
GAAGATGAAA GTGCGCAAAC AGGTGGCAGG AAACGTGGCA TGTCTCGGAG GCTGGCATCA
CTGAGGCACT GTACGGTCCT TGCAATGTCA AACTTACTCA ATGCCAACGT AGACAGTGGT
CTCATGCACT CCATAGGCTT AGGTTACCAC AAGGATCTCC AGACAAGAGC TACATTTATG
GAAGTTCTGA CAAAAATCCT TCAACAAGGC ACAGAATTTG ACACACTTGC AGAAACAGTA
TTGGCTGATC GGTTTGAGAG ATTGGTGGAA CTGGTCACAA TGATGGGTGA TCAAGGAGAA
CTCCCTATAG CGATGGCTCT GGCCAATGTG GTTCCTTGTT CTCAGTGGGA TGAACTAGCT
CGAGTTCTGG TTACTCTGTT TGATTCTCGG CATTTACTCT ACCAACTGCT CTGGAACATG
TTTTCTAAAG AAGTAGAATT GGCAGACTCC ATGCAGACTC TCTTCCGAGG CAACAGCTTG
GCCAGTAAAA TAATGACATT CTGTTTCAAG GTATATGGTG CTACCTATCT ACAAAAACTC
CTGGATCCTT TATTACGAAT TGTGATCACA TCCTCTGATT GGCAACATGT TAGCTTTGAA
GTGGATCCTA CCAGGTTAGA ACCATCAGAG AGCCTTGAGG AAAACCAGCG GAACCTCCTT
CAGATGACTG AAAAGTTCTT CCATGCCATC ATCAGTTCCT CCTCAGAATT CCCCCCTCAA
CTTCGAAGTG TGTGCCACTG TTTATACCAG GTGGTTAGCC AGCGTTTCCC TCAGAACAGC
ATCGGTGCAG TAGGAAGTGC CATGTTCCTC AGATTTATCA ATCCTGCCAT TGTCTCACCG
TATGAAGCAG GGATTTTAGA TAAAAAGCCA CCACCTAGAA TCGAAAGGGG CTTGAAGTTA
ATGTCAAAGA TACTTCAGAG TATTGCCAAT CATGTTCTCT TCACAAAAGA AGAACATATG
CGGCCTTTCA ATGATTTTGT GAAAAGCAAC TTTGATGCAG CACGCAGGTT TTTCCTTGAT
ATAGCATCTG ATTGTCCTAC AAGTGATGCA GTAAATCATA GTCTTTCCTT CATAAGTGAC
GGCAATGTGC TTGCTTTACA TCGTCTACTC TGGAACAATC AGGAGAAAAT TGGGCAGTAT
CTTTCCAGCA ACAGGGATCA TAAAGCTGTT GGAAGACGAC CTTTTGATAA GATGGCAACA
CTTCTTGCAT ACCTGGGTCC TCCAGAGCAC AAACCTGTGG CAGATACACA CTGGTCCAGC
CTTAACCTTA CCAGTTCAAA GTTTGAGGAA TTTATGACTA GGCATCAGGT ACATGAAAAA
GAAGAATTCA AGGCTTTGAA AACGTTAAGT ATTTTCTACC AAGCTGGGAC TTCCAAAGCT
GGGAATCCTA TTTTTTATTA TGTTGCACGG AGGTTCAAAA CTGGTCAAAT CAATGGTGAT
TTGCTGATAT ACCATGTCTT ACTGACTTTA AAGCCATATT ATGCAAAGCC ATATGAAATT
GTAGTGGACC TTACCCATAC CGGGCCTAGC AATCGCTTTA AAACAGACTT TCTCTCTAAG
TGGTTTGTTG TTTTTCCTGG CTTTGCTTAC GACAACGTCT CCGCAGTCTA TATCTATAAC
TGTAACTCCT GGGTCAGGGA GTACACCAAG TATCATGAGC GGCTGCTGAC TGGCCTCAAA
GGTAGCAAAA GGCTTGTTTT CATAGACTGT CCTGGGAAAC TGGCTGAGCA CATAGAGCAT
GAACAACAGA AACTACCTGC TGCCACCTTG GCTTTAGAAG AGGACCTGAA GGTATTCCAC
AATGCTCTCA AGCTAGCTCA CAAAGACACC AAAGTTTCTA TTAAAGTTGG TTCTACTGCT
GTCCAAGTAA CTTCAGCAGA GCGAACAAAA GTCCTAGGGC AATCAGTCTT TCTAAATGAC
ATTTATTATG CTTCGGAAAT TGAAGAAATC TGCCTAGTAG ATGAGAACCA GTTCACCTTA
ACCATTGCAA ACCAGGGCAC GCCGCTCACC TTCATGCACC AGGAGTGTGA AGCCATTGTC
CAGTCTATCA TTCATATCCG GACCCGCTGG GAACTGTCAC AGCCCGACTC TATCCCCCAA
CACACCAAGA TTCGGCCAAA AGATGTCCCT GGGACACTGC TCAATATCGC ATTACTTAAT
TTAGGCAGTT CTGACCCGAG TTTACGGTCA GCTGCCTATA ATCTTCTGTG TGCCTTAACT
TGTACCTTTA ATTTAAAAAT CGAGGGCCAG TTACTAGAGA CATCAGGTTT ATGTATCCCT
GCCAACAACA CCCTCTTTAT TGTCTCTATT AGTAAGACAC TGGCAGCCAA TGAGCCACAC
CTCACGTTAG AATTTTTGGA AGAGTGTATT TCTGGATTTA GCAAATCTAG TATTGAATTG
AAACACCTTT GTTTGGAATA CATGACTCCA TGGCTGTCAA ATCTAGTTCG TTTTTGCAAG
CATAATGATG ATGCCAAACG ACAAAGAGTT ACTGCTATTC TTGACAAGCT GATAACAATG
ACCATCAATG AAAAACAGAT GTACCCATCT ATTCAAGCAA AAATATGGGG AAGCCTTGGG
CAGATTACAG ATCTGCTTGA TGTTGTACTA GACAGTTTCA TCAAAACCAG TGCAACAGGT
GGCTTGGGAT CAATAAAAGC TGAGGTGATG GCAGATACTG CTGTAGCTTT GGCTTCTGGA
AATGTGAAAT TGGTTTCAAG CAAGGTTATT GGAAGGATGT GCAAAATAAT TGACAAGACA
TGCTTATCTC CAACTCCTAC TTTAGAACAA CATCTTATGT GGGATGATAT TGCTATTTTA
GCACGCTACA TGCTGATGCT GTCCTTCAAC AATTCCCTTG ATGTGGCAGC TCATCTTCCC
TACCTCTTCC ACGTTGTTAC TTTCTTAGTA GCCACAGGTC CGCTCTCCCT TAGAGCTTCC
ACACATGGAC TGGTCATTAA TATCATTCAC TCTCTGTGTA CTTGTTCACA GCTTCATTTT
AGTGAAGAGA CCAAGCAAGT TTTGAGACTC AGTCTGACAG AGTTCTCATT ACCCAAATTT
TACTTGCTGT TTGGCATTAG CAAAGTCAAG TCAGCTGCTG TCATTGCCTT CCGTTCCAGT
TACCGGGACA GGTCATTCTC TCCTGGCTCC TATGAGAGAG AGACTTTTGC TTTGACATCC
TTGGAAACAG TCACAGAAGC TTTGTTGGAG ATCATGGAGG CATGCATGAG AGATATTCCA
ACGTGCAAGT GGCTGGACCA GTGGACAGAA CTAGCTCAAA GATTTGCATT CCAATATAAT
CCATCCCTGC AACCAAGAGC TCTTGTTGTC TTTGGGTGTA TTAGCAAACG AGTGTCTCAT
GGGCAGATAA AGCAGATAAT CCGTATTCTT AGCAAGGCAC TTGAGAGTTG CTTAAAAGGA
CCTGACACTT ACAACAGTCA AGTTCTGATA GAAGCTACAG TAATAGCACT AACCAAATTA
CAGCCACTTC TTAATAAGGA CTCGCCTCTG CACAAAGCCC TCTTTTGGGT AGCTGTGGCT
GTGCTGCAGC TTGATGAGGT CAACTTGTAT TCAGCAGGTA CCGCACTTCT TGAACAAAAC
CTGCATACTT TAGATAGTCT CCGTATATTC AATGACAAGA GTCCAGAGGA AGTATTTATG
GCAATCCGGA ATCCTCTGGA GTGGCACTGC AAGCAAATGG ATCATTTTGT TGGACTCAAT
TTCAACTCTA ACTTTAACTT TGCATTGGTT GGACACCTTT TAAAAGGGTA CAGGCATCCT
TCACCTGCTA TTGTTGCAAG AACAGTCAGA ATTTTACATA CACTACTAAC TCTGGTTAAC
AAACACAGAA ATTGTGACAA ATTTGAAGTG AATACACAGA GCGTGGCCTA CTTAGCAGCT
TTACTTACAG TGTCTGAAGA AGTTCGAAGT CGCTGCAGCC TAAAACATAG AAAGTCACTT
CTTCTTACTG ATATTTCAAT GGAAAATGTT CCTATGGATA CATATCCCAT TCATCATGGT
GACCCTTCCT ATAGGACACT AAAGGAGACT CAGCCATGGT CCTCTCCCAA AGGTTCTGAA
GGATACCTTG CAGCCACCTA TCCAACTGTC GGCCAGACCA GTCCCCGAGC CAGGAAATCC
ATGAGCCTGG ACATGGGGCA ACCTTCTCAG GCCAACACTA AGAAGTTGCT TGGAACAAGG
AAAAGTTTTG ATCACTTGAT ATCAGACACA AAGGCTCCTA AAAGGCAAGA AATGGAATCA
GGGATCACAA CACCCCCCAA AATGAGGAGA GTAGCAGAAA CTGATTATGA AATGGAAACT
CAGAGGATTT CCTCATCACA ACAGCACCCA CATTTACGTA AAGTTTCAGT GTCTGAATCA
AATGTTCTCT TGGATGAAGA AGTACTTACT GATCCGAAGA TCCAGGCGCT GCTTCTTACT
GTTCTAGCTA CACTGGTAAA ATATACCACA GATGAGTTTG ATCAACGAAT TCTTTATGAA
TACTTAGCAG AGGCCAGTGT TGTGTTTCCC AAAGTCTTTC CTGTTGTGCA TAATTTGTTG
GACTCTAAGA TCAACACCCT GTTATCATTG TGCCAAGATC CAAATTTGTT AAATCCAATC
CATGGAATTG TGCAGAGTGT GGTGTACCAT GAAGAATCCC CACCACAATA CCAAACATCT
TACCTGCAAA GTTTTGGTTT TAATGGCTTG TGGCGGTTTG CAGGACCGTT TTCAAAGCAA
ACACAAATTC CAGACTATGC TGAGCTTATT GTTAAGTTTC TTGATGCCTT GATTGACACG
TACCTGCCTG GAATTGATGA AGAAACCAGT GAAGAATCCC TCCTGACTCC CACATCTCCT
TACCCTCCTG CACTGCAGAG CCAGCTTAGT ATCACTGCCA ACCTTAACCT TTCTAATTCC
ATGACCTCAC TTGCAACTTC CCAGCATTCC CCAGCTTCTC TGCCTTGCTC TAAATCAGCA
GTTTTCATGC AGCTGTTCCC TCATCAAGGA ATCGACAAGG AGAACGTTGA ACTCTCCCCT
ACCACTGGCC ACTGTAACAG TGGACGAACT CGCCACGGAT CCGCAAGCCA AGTGCAGAAG
CAAAGAAGCG CTGGCAGTTT CAAACGTAAT AGCATTAAGA AGATCGTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59246
Clone ID Related Accession (Same CDS sequence) XM_011524857
Accession Version XM_011524857.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8481bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neurofibromin isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
CGGAGGTTCA AAACTGGTCA AATCAATGGT GATTTGCTGA TATACCATGT CTTACTGACT
TTAAAGCCAT ATTATGCAAA GCCATATGAA ATTGTAGTGG ACCTTACCCA TACCGGGCCT
AGCAATCGCT TTAAAACAGA CTTTCTCTCT AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT
TACGACAACG TCTCCGCAGT CTATATCTAT AACTGTAACT CCTGGGTCAG GGAGTACACC
AAGTATCATG AGCGGCTGCT GACTGGCCTC AAAGGTAGCA AAAGGCTTGT TTTCATAGAC
TGTCCTGGGA AACTGGCTGA GCACATAGAG CATGAACAAC AGAAACTACC TGCTGCCACC
TTGGCTTTAG AAGAGGACCT GAAGGTATTC CACAATGCTC TCAAGCTAGC TCACAAAGAC
ACCAAAGTTT CTATTAAAGT TGGTTCTACT GCTGTCCAAG TAACTTCAGC AGAGCGAACA
AAAGTCCTAG GGCAATCAGT CTTTCTAAAT GACATTTATT ATGCTTCGGA AATTGAAGAA
ATCTGCCTAG TAGATGAGAA CCAGTTCACC TTAACCATTG CAAACCAGGG CACGCCGCTC
ACCTTCATGC ACCAGGAGTG TGAAGCCATT GTCCAGTCTA TCATTCATAT CCGGACCCGC
TGGGAACTGT CACAGCCCGA CTCTATCCCC CAACACACCA AGATTCGGCC AAAAGATGTC
CCTGGGACAC TGCTCAATAT CGCATTACTT AATTTAGGCA GTTCTGACCC GAGTTTACGG
TCAGCTGCCT ATAATCTTCT GTGTGCCTTA ACTTGTACCT TTAATTTAAA AATCGAGGGC
CAGTTACTAG AGACATCAGG TTTATGTATC CCTGCCAACA ACACCCTCTT TATTGTCTCT
ATTAGTAAGA CACTGGCAGC CAATGAGCCA CACCTCACGT TAGAATTTTT GGAAGAGTGT
ATTTCTGGAT TTAGCAAATC TAGTATTGAA TTGAAACACC TTTGTTTGGA ATACATGACT
CCATGGCTGT CAAATCTAGT TCGTTTTTGC AAGCATAATG ATGATGCCAA ACGACAAAGA
GTTACTGCTA TTCTTGACAA GCTGATAACA ATGACCATCA ATGAAAAACA GATGTACCCA
TCTATTCAAG CAAAAATATG GGGAAGCCTT GGGCAGATTA CAGATCTGCT TGATGTTGTA
CTAGACAGTT TCATCAAAAC CAGTGCAACA GGTGGCTTGG GATCAATAAA AGCTGAGGTG
ATGGCAGATA CTGCTGTAGC TTTGGCTTCT GGAAATGTGA AATTGGTTTC AAGCAAGGTT
ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACATGCTTAT CTCCAACTCC TACTTTAGAA
CAACATCTTA TGTGGGATGA TATTGCTATT TTAGCACGCT ACATGCTGAT GCTGTCCTTC
AACAATTCCC TTGATGTGGC AGCTCATCTT CCCTACCTCT TCCACGTTGT TACTTTCTTA
GTAGCCACAG GTCCGCTCTC CCTTAGAGCT TCCACACATG GACTGGTCAT TAATATCATT
CACTCTCTGT GTACTTGTTC ACAGCTTCAT TTTAGTGAAG AGACCAAGCA AGTTTTGAGA
CTCAGTCTGA CAGAGTTCTC ATTACCCAAA TTTTACTTGC TGTTTGGCAT TAGCAAAGTC
AAGTCAGCTG CTGTCATTGC CTTCCGTTCC AGTTACCGGG ACAGGTCATT CTCTCCTGGC
TCCTATGAGA GAGAGACTTT TGCTTTGACA TCCTTGGAAA CAGTCACAGA AGCTTTGTTG
GAGATCATGG AGGCATGCAT GAGAGATATT CCAACGTGCA AGTGGCTGGA CCAGTGGACA
GAACTAGCTC AAAGATTTGC ATTCCAATAT AATCCATCCC TGCAACCAAG AGCTCTTGTT
GTCTTTGGGT GTATTAGCAA ACGAGTGTCT CATGGGCAGA TAAAGCAGAT AATCCGTATT
CTTAGCAAGG CACTTGAGAG TTGCTTAAAA GGACCTGACA CTTACAACAG TCAAGTTCTG
ATAGAAGCTA CAGTAATAGC ACTAACCAAA TTACAGCCAC TTCTTAATAA GGACTCGCCT
CTGCACAAAG CCCTCTTTTG GGTAGCTGTG GCTGTGCTGC AGCTTGATGA GGTCAACTTG
TATTCAGCAG GTACCGCACT TCTTGAACAA AACCTGCATA CTTTAGATAG TCTCCGTATA
TTCAATGACA AGAGTCCAGA GGAAGTATTT ATGGCAATCC GGAATCCTCT GGAGTGGCAC
TGCAAGCAAA TGGATCATTT TGTTGGACTC AATTTCAACT CTAACTTTAA CTTTGCATTG
GTTGGACACC TTTTAAAAGG GTACAGGCAT CCTTCACCTG CTATTGTTGC AAGAACAGTC
AGAATTTTAC ATACACTACT AACTCTGGTT AACAAACACA GAAATTGTGA CAAATTTGAA
GTGAATACAC AGAGCGTGGC CTACTTAGCA GCTTTACTTA CAGTGTCTGA AGAAGTTCGA
AGTCGCTGCA GCCTAAAACA TAGAAAGTCA CTTCTTCTTA CTGATATTTC AATGGAAAAT
GTTCCTATGG ATACATATCC CATTCATCAT GGTGACCCTT CCTATAGGAC ACTAAAGGAG
ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT GAAGGATACC TTGCAGCCAC CTATCCAACT
GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA TCCATGAGCC TGGACATGGG GCAACCTTCT
CAGGCCAACA CTAAGAAGTT GCTTGAAACT CAGAGGATTT CCTCATCACA ACAGCACCCA
CATTTACGTA AAGTTTCAGT GTCTGAATCA AATGTTCTCT TGGATGAAGA AGTACTTACT
GATCCGAAGA TCCAGGCGCT GCTTCTTACT GTTCTAGCTA CACTGGTAAA ATATACCACA
GATGAGTTTG ATCAACGAAT TCTTTATGAA TACTTAGCAG AGGCCAGTGT TGTGTTTCCC
AAAGTCTTTC CTGTTGTGCA TAATTTGTTG GACTCTAAGA TCAACACCCT GTTATCATTG
TGCCAAGATC CAAATTTGTT AAATCCAATC CATGGAATTG TGCAGAGTGT GGTGTACCAT
GAAGAATCCC CACCACAATA CCAAACATCT TACCTGCAAA GTTTTGGTTT TAATGGCTTG
TGGCGGTTTG CAGGACCGTT TTCAAAGCAA ACACAAATTC CAGACTATGC TGAGCTTATT
GTTAAGTTTC TTGATGCCTT GATTGACACG TACCTGCCTG GAATTGATGA AGAAACCAGT
GAAGAATCCC TCCTGACTCC CACATCTCCT TACCCTCCTG CACTGCAGAG CCAGCTTAGT
ATCACTGCCA ACCTTAACCT TTCTAATTCC ATGACCTCAC TTGCAACTTC CCAGCATTCC
CCAGCTTCTC TGCCTTGCTC TAAATCAGCA GTTTTCATGC AGCTGTTCCC TCATCAAGGA
ATCGACAAGG AGAACGTTGA ACTCTCCCCT ACCACTGGCC ACTGTAACAG TGGACGAACT
CGCCACGGAT CCGCAAGCCA AGTGCAGAAG CAAAGAAGCG CTGGCAGTTT CAAACGTAAT
AGCATTAAGA AGATCGTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523159.1
CDS334..8814
Misc Feature(1)3922..5034(+)
Misc Feature(2)3955..5016(+)
Misc Feature(3)4069..4737(+)
Misc Feature(4)5104..5547(+)
Misc Feature(5)5173..5478(+)
Misc Feature(6)5377..5475(+)
Misc Feature(7)5545..5874(+)
Misc Feature(8)5545..5874(+)
Translation

Target ORF information:

RefSeq Version XM_011524857
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524857

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
CGGAGGTTCA AAACTGGTCA AATCAATGGT GATTTGCTGA TATACCATGT CTTACTGACT
TTAAAGCCAT ATTATGCAAA GCCATATGAA ATTGTAGTGG ACCTTACCCA TACCGGGCCT
AGCAATCGCT TTAAAACAGA CTTTCTCTCT AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT
TACGACAACG TCTCCGCAGT CTATATCTAT AACTGTAACT CCTGGGTCAG GGAGTACACC
AAGTATCATG AGCGGCTGCT GACTGGCCTC AAAGGTAGCA AAAGGCTTGT TTTCATAGAC
TGTCCTGGGA AACTGGCTGA GCACATAGAG CATGAACAAC AGAAACTACC TGCTGCCACC
TTGGCTTTAG AAGAGGACCT GAAGGTATTC CACAATGCTC TCAAGCTAGC TCACAAAGAC
ACCAAAGTTT CTATTAAAGT TGGTTCTACT GCTGTCCAAG TAACTTCAGC AGAGCGAACA
AAAGTCCTAG GGCAATCAGT CTTTCTAAAT GACATTTATT ATGCTTCGGA AATTGAAGAA
ATCTGCCTAG TAGATGAGAA CCAGTTCACC TTAACCATTG CAAACCAGGG CACGCCGCTC
ACCTTCATGC ACCAGGAGTG TGAAGCCATT GTCCAGTCTA TCATTCATAT CCGGACCCGC
TGGGAACTGT CACAGCCCGA CTCTATCCCC CAACACACCA AGATTCGGCC AAAAGATGTC
CCTGGGACAC TGCTCAATAT CGCATTACTT AATTTAGGCA GTTCTGACCC GAGTTTACGG
TCAGCTGCCT ATAATCTTCT GTGTGCCTTA ACTTGTACCT TTAATTTAAA AATCGAGGGC
CAGTTACTAG AGACATCAGG TTTATGTATC CCTGCCAACA ACACCCTCTT TATTGTCTCT
ATTAGTAAGA CACTGGCAGC CAATGAGCCA CACCTCACGT TAGAATTTTT GGAAGAGTGT
ATTTCTGGAT TTAGCAAATC TAGTATTGAA TTGAAACACC TTTGTTTGGA ATACATGACT
CCATGGCTGT CAAATCTAGT TCGTTTTTGC AAGCATAATG ATGATGCCAA ACGACAAAGA
GTTACTGCTA TTCTTGACAA GCTGATAACA ATGACCATCA ATGAAAAACA GATGTACCCA
TCTATTCAAG CAAAAATATG GGGAAGCCTT GGGCAGATTA CAGATCTGCT TGATGTTGTA
CTAGACAGTT TCATCAAAAC CAGTGCAACA GGTGGCTTGG GATCAATAAA AGCTGAGGTG
ATGGCAGATA CTGCTGTAGC TTTGGCTTCT GGAAATGTGA AATTGGTTTC AAGCAAGGTT
ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACATGCTTAT CTCCAACTCC TACTTTAGAA
CAACATCTTA TGTGGGATGA TATTGCTATT TTAGCACGCT ACATGCTGAT GCTGTCCTTC
AACAATTCCC TTGATGTGGC AGCTCATCTT CCCTACCTCT TCCACGTTGT TACTTTCTTA
GTAGCCACAG GTCCGCTCTC CCTTAGAGCT TCCACACATG GACTGGTCAT TAATATCATT
CACTCTCTGT GTACTTGTTC ACAGCTTCAT TTTAGTGAAG AGACCAAGCA AGTTTTGAGA
CTCAGTCTGA CAGAGTTCTC ATTACCCAAA TTTTACTTGC TGTTTGGCAT TAGCAAAGTC
AAGTCAGCTG CTGTCATTGC CTTCCGTTCC AGTTACCGGG ACAGGTCATT CTCTCCTGGC
TCCTATGAGA GAGAGACTTT TGCTTTGACA TCCTTGGAAA CAGTCACAGA AGCTTTGTTG
GAGATCATGG AGGCATGCAT GAGAGATATT CCAACGTGCA AGTGGCTGGA CCAGTGGACA
GAACTAGCTC AAAGATTTGC ATTCCAATAT AATCCATCCC TGCAACCAAG AGCTCTTGTT
GTCTTTGGGT GTATTAGCAA ACGAGTGTCT CATGGGCAGA TAAAGCAGAT AATCCGTATT
CTTAGCAAGG CACTTGAGAG TTGCTTAAAA GGACCTGACA CTTACAACAG TCAAGTTCTG
ATAGAAGCTA CAGTAATAGC ACTAACCAAA TTACAGCCAC TTCTTAATAA GGACTCGCCT
CTGCACAAAG CCCTCTTTTG GGTAGCTGTG GCTGTGCTGC AGCTTGATGA GGTCAACTTG
TATTCAGCAG GTACCGCACT TCTTGAACAA AACCTGCATA CTTTAGATAG TCTCCGTATA
TTCAATGACA AGAGTCCAGA GGAAGTATTT ATGGCAATCC GGAATCCTCT GGAGTGGCAC
TGCAAGCAAA TGGATCATTT TGTTGGACTC AATTTCAACT CTAACTTTAA CTTTGCATTG
GTTGGACACC TTTTAAAAGG GTACAGGCAT CCTTCACCTG CTATTGTTGC AAGAACAGTC
AGAATTTTAC ATACACTACT AACTCTGGTT AACAAACACA GAAATTGTGA CAAATTTGAA
GTGAATACAC AGAGCGTGGC CTACTTAGCA GCTTTACTTA CAGTGTCTGA AGAAGTTCGA
AGTCGCTGCA GCCTAAAACA TAGAAAGTCA CTTCTTCTTA CTGATATTTC AATGGAAAAT
GTTCCTATGG ATACATATCC CATTCATCAT GGTGACCCTT CCTATAGGAC ACTAAAGGAG
ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT GAAGGATACC TTGCAGCCAC CTATCCAACT
GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA TCCATGAGCC TGGACATGGG GCAACCTTCT
CAGGCCAACA CTAAGAAGTT GCTTGAAACT CAGAGGATTT CCTCATCACA ACAGCACCCA
CATTTACGTA AAGTTTCAGT GTCTGAATCA AATGTTCTCT TGGATGAAGA AGTACTTACT
GATCCGAAGA TCCAGGCGCT GCTTCTTACT GTTCTAGCTA CACTGGTAAA ATATACCACA
GATGAGTTTG ATCAACGAAT TCTTTATGAA TACTTAGCAG AGGCCAGTGT TGTGTTTCCC
AAAGTCTTTC CTGTTGTGCA TAATTTGTTG GACTCTAAGA TCAACACCCT GTTATCATTG
TGCCAAGATC CAAATTTGTT AAATCCAATC CATGGAATTG TGCAGAGTGT GGTGTACCAT
GAAGAATCCC CACCACAATA CCAAACATCT TACCTGCAAA GTTTTGGTTT TAATGGCTTG
TGGCGGTTTG CAGGACCGTT TTCAAAGCAA ACACAAATTC CAGACTATGC TGAGCTTATT
GTTAAGTTTC TTGATGCCTT GATTGACACG TACCTGCCTG GAATTGATGA AGAAACCAGT
GAAGAATCCC TCCTGACTCC CACATCTCCT TACCCTCCTG CACTGCAGAG CCAGCTTAGT
ATCACTGCCA ACCTTAACCT TTCTAATTCC ATGACCTCAC TTGCAACTTC CCAGCATTCC
CCAGCTTCTC TGCCTTGCTC TAAATCAGCA GTTTTCATGC AGCTGTTCCC TCATCAAGGA
ATCGACAAGG AGAACGTTGA ACTCTCCCCT ACCACTGGCC ACTGTAACAG TGGACGAACT
CGCCACGGAT CCGCAAGCCA AGTGCAGAAG CAAAGAAGCG CTGGCAGTTT CAAACGTAAT
AGCATTAAGA AGATCGTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50433
Clone ID Related Accession (Same CDS sequence) XM_006721926 , XM_006721927
Accession Version XM_006721926.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8472bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neurofibromin isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578830998. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
CGGAGGTTCA AAACTGGTCA AATCAATGGT GATTTGCTGA TATACCATGT CTTACTGACT
TTAAAGCCAT ATTATGCAAA GCCATATGAA ATTGTAGTGG ACCTTACCCA TACCGGGCCT
AGCAATCGCT TTAAAACAGA CTTTCTCTCT AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT
TACGACAACG TCTCCGCAGT CTATATCTAT AACTGTAACT CCTGGGTCAG GGAGTACACC
AAGTATCATG AGCGGCTGCT GACTGGCCTC AAAGGTAGCA AAAGGCTTGT TTTCATAGAC
TGTCCTGGGA AACTGGCTGA GCACATAGAG CATGAACAAC AGAAACTACC TGCTGCCACC
TTGGCTTTAG AAGAGGACCT GAAGGTATTC CACAATGCTC TCAAGCTAGC TCACAAAGAC
ACCAAAGTTT CTATTAAAGT TGGTTCTACT GCTGTCCAAG TAACTTCAGC AGAGCGAACA
AAAGTCCTAG GGCAATCAGT CTTTCTAAAT GACATTTATT ATGCTTCGGA AATTGAAGAA
ATCTGCCTAG TAGATGAGAA CCAGTTCACC TTAACCATTG CAAACCAGGG CACGCCGCTC
ACCTTCATGC ACCAGGAGTG TGAAGCCATT GTCCAGTCTA TCATTCATAT CCGGACCCGC
TGGGAACTGT CACAGCCCGA CTCTATCCCC CAACACACCA AGATTCGGCC AAAAGATGTC
CCTGGGACAC TGCTCAATAT CGCATTACTT AATTTAGGCA GTTCTGACCC GAGTTTACGG
TCAGCTGCCT ATAATCTTCT GTGTGCCTTA ACTTGTACCT TTAATTTAAA AATCGAGGGC
CAGTTACTAG AGACATCAGG TTTATGTATC CCTGCCAACA ACACCCTCTT TATTGTCTCT
ATTAGTAAGA CACTGGCAGC CAATGAGCCA CACCTCACGT TAGAATTTTT GGAAGAGTGT
ATTTCTGGAT TTAGCAAATC TAGTATTGAA TTGAAACACC TTTGTTTGGA ATACATGACT
CCATGGCTGT CAAATCTAGT TCGTTTTTGC AAGCATAATG ATGATGCCAA ACGACAAAGA
GTTACTGCTA TTCTTGACAA GCTGATAACA ATGACCATCA ATGAAAAACA GATGTACCCA
TCTATTCAAG CAAAAATATG GGGAAGCCTT GGGCAGATTA CAGATCTGCT TGATGTTGTA
CTAGACAGTT TCATCAAAAC CAGTGCAACA GGTGGCTTGG GATCAATAAA AGCTGAGGTG
ATGGCAGATA CTGCTGTAGC TTTGGCTTCT GGAAATGTGA AATTGGTTTC AAGCAAGGTT
ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACATGCTTAT CTCCAACTCC TACTTTAGAA
CAACATCTTA TGTGGGATGA TATTGCTATT TTAGCACGCT ACATGCTGAT GCTGTCCTTC
AACAATTCCC TTGATGTGGC AGCTCATCTT CCCTACCTCT TCCACGTTGT TACTTTCTTA
GTAGCCACAG GTCCGCTCTC CCTTAGAGCT TCCACACATG GACTGGTCAT TAATATCATT
CACTCTCTGT GTACTTGTTC ACAGCTTCAT TTTAGTGAAG AGACCAAGCA AGTTTTGAGA
CTCAGTCTGA CAGAGTTCTC ATTACCCAAA TTTTACTTGC TGTTTGGCAT TAGCAAAGTC
AAGTCAGCTG CTGTCATTGC CTTCCGTTCC AGTTACCGGG ACAGGTCATT CTCTCCTGGC
TCCTATGAGA GAGAGACTTT TGCTTTGACA TCCTTGGAAA CAGTCACAGA AGCTTTGTTG
GAGATCATGG AGGCATGCAT GAGAGATATT CCAACGTGCA AGTGGCTGGA CCAGTGGACA
GAACTAGCTC AAAGATTTGC ATTCCAATAT AATCCATCCC TGCAACCAAG AGCTCTTGTT
GTCTTTGGGT GTATTAGCAA ACGAGTGTCT CATGGGCAGA TAAAGCAGAT AATCCGTATT
CTTAGCAAGG CACTTGAGAG TTGCTTAAAA GGACCTGACA CTTACAACAG TCAAGTTCTG
ATAGAAGCTA CAGTAATAGC ACTAACCAAA TTACAGCCAC TTCTTAATAA GGACTCGCCT
CTGCACAAAG CCCTCTTTTG GGTAGCTGTG GCTGTGCTGC AGCTTGATGA GGTCAACTTG
TATTCAGCAG GTACCGCACT TCTTGAACAA AACCTGCATA CTTTAGATAG TCTCCGTATA
TTCAATGACA AGAGTCCAGA GGAAGTATTT ATGGCAATCC GGAATCCTCT GGAGTGGCAC
TGCAAGCAAA TGGATCATTT TGTTGGACTC AATTTCAACT CTAACTTTAA CTTTGCATTG
GTTGGACACC TTTTAAAAGG GTACAGGCAT CCTTCACCTG CTATTGTTGC AAGAACAGTC
AGAATTTTAC ATACACTACT AACTCTGGTT AACAAACACA GAAATTGTGA CAAATTTGAA
GTGAATACAC AGAGCGTGGC CTACTTAGCA GCTTTACTTA CAGTGTCTGA AGAAGTTCGA
AGTCGCTGCA GCCTAAAACA TAGAAAGTCA CTTCTTCTTA CTGATATTTC AATGGAAAAT
GTTCCTATGG ATACATATCC CATTCATCAT GGTGACCCTT CCTATAGGAC ACTAAAGGAG
ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT GAAGGATACC TTGCAGCCAC CTATCCAACT
GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA TCCATGAGCC TGGACATGGG GCAACCTTCT
CAGGCCAACA CTAAGAAGTT GCTTGGAACA AGGAAAAGTT TTGATCACTT GATATCAGAC
ACAAAGGCTC CTAAAAGGCA AGAAATGGAA TCAGGGATCA CAACACCCCC CAAAATGAGG
AGAGTAGCAG AAACTGATTA TGAAATGGAA ACTCAGAGGA TTTCCTCATC ACAACAGCAC
CCACATTTAC GTAAAGTTTC AGTGTCTGAA TCAAATGTTC TCTTGGATGA AGAAGTACTT
ACTGATCCGA AGATCCAGGC GCTGCTTCTT ACTGTTCTAG CTACACTGGT AAAATATACC
ACAGATGAGT TTGATCAACG AATTCTTTAT GAATACTTAG CAGAGGCCAG TGTTGTGTTT
CCCAAAGTCT TTCCTGTTGT GCATAATTTG TTGGACTCTA AGATCAACAC CCTGTTATCA
TTGTGCCAAG ATCCAAATTT GTTAAATCCA ATCCATGGAA TTGTGCAGAG TGTGGTGTAC
CATGAAGAAT CCCCACCACA ATACCAAACA TCTTACCTGC AAAGTTTTGG TTTTAATGGC
TTGTGGCGGT TTGCAGGACC GTTTTCAAAG CAAACACAAA TTCCAGACTA TGCTGAGCTT
ATTGTTAAGT TTCTTGATGC CTTGATTGAC ACGTACCTGC CTGGAATTGA TGAAGAAACC
AGTGAAGAAT CCCTCCTGAC TCCCACATCT CCTTACCCTC CTGCACTGCA GAGCCAGCTT
AGTATCACTG CCAACCTTAA CCTTTCTAAT TCCATGACCT CACTTGCAAC TTCCCAGCAT
TCCCCAGATA CTGCAGCCAC TGAATTCCTT CCTGCTTCCC CGCTGATAAA CCTTTTAGCA
TTTTGGAGCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006721989.1
CDS334..8805
Misc Feature(1)3922..5034(+)
Misc Feature(2)3955..5016(+)
Misc Feature(3)4069..4737(+)
Misc Feature(4)5104..5547(+)
Misc Feature(5)5173..5478(+)
Misc Feature(6)5377..5475(+)
Misc Feature(7)5545..5874(+)
Misc Feature(8)5545..5874(+)
Translation

Target ORF information:

RefSeq Version XM_006721926
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X13, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006721926

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
CGGAGGTTCA AAACTGGTCA AATCAATGGT GATTTGCTGA TATACCATGT CTTACTGACT
TTAAAGCCAT ATTATGCAAA GCCATATGAA ATTGTAGTGG ACCTTACCCA TACCGGGCCT
AGCAATCGCT TTAAAACAGA CTTTCTCTCT AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT
TACGACAACG TCTCCGCAGT CTATATCTAT AACTGTAACT CCTGGGTCAG GGAGTACACC
AAGTATCATG AGCGGCTGCT GACTGGCCTC AAAGGTAGCA AAAGGCTTGT TTTCATAGAC
TGTCCTGGGA AACTGGCTGA GCACATAGAG CATGAACAAC AGAAACTACC TGCTGCCACC
TTGGCTTTAG AAGAGGACCT GAAGGTATTC CACAATGCTC TCAAGCTAGC TCACAAAGAC
ACCAAAGTTT CTATTAAAGT TGGTTCTACT GCTGTCCAAG TAACTTCAGC AGAGCGAACA
AAAGTCCTAG GGCAATCAGT CTTTCTAAAT GACATTTATT ATGCTTCGGA AATTGAAGAA
ATCTGCCTAG TAGATGAGAA CCAGTTCACC TTAACCATTG CAAACCAGGG CACGCCGCTC
ACCTTCATGC ACCAGGAGTG TGAAGCCATT GTCCAGTCTA TCATTCATAT CCGGACCCGC
TGGGAACTGT CACAGCCCGA CTCTATCCCC CAACACACCA AGATTCGGCC AAAAGATGTC
CCTGGGACAC TGCTCAATAT CGCATTACTT AATTTAGGCA GTTCTGACCC GAGTTTACGG
TCAGCTGCCT ATAATCTTCT GTGTGCCTTA ACTTGTACCT TTAATTTAAA AATCGAGGGC
CAGTTACTAG AGACATCAGG TTTATGTATC CCTGCCAACA ACACCCTCTT TATTGTCTCT
ATTAGTAAGA CACTGGCAGC CAATGAGCCA CACCTCACGT TAGAATTTTT GGAAGAGTGT
ATTTCTGGAT TTAGCAAATC TAGTATTGAA TTGAAACACC TTTGTTTGGA ATACATGACT
CCATGGCTGT CAAATCTAGT TCGTTTTTGC AAGCATAATG ATGATGCCAA ACGACAAAGA
GTTACTGCTA TTCTTGACAA GCTGATAACA ATGACCATCA ATGAAAAACA GATGTACCCA
TCTATTCAAG CAAAAATATG GGGAAGCCTT GGGCAGATTA CAGATCTGCT TGATGTTGTA
CTAGACAGTT TCATCAAAAC CAGTGCAACA GGTGGCTTGG GATCAATAAA AGCTGAGGTG
ATGGCAGATA CTGCTGTAGC TTTGGCTTCT GGAAATGTGA AATTGGTTTC AAGCAAGGTT
ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACATGCTTAT CTCCAACTCC TACTTTAGAA
CAACATCTTA TGTGGGATGA TATTGCTATT TTAGCACGCT ACATGCTGAT GCTGTCCTTC
AACAATTCCC TTGATGTGGC AGCTCATCTT CCCTACCTCT TCCACGTTGT TACTTTCTTA
GTAGCCACAG GTCCGCTCTC CCTTAGAGCT TCCACACATG GACTGGTCAT TAATATCATT
CACTCTCTGT GTACTTGTTC ACAGCTTCAT TTTAGTGAAG AGACCAAGCA AGTTTTGAGA
CTCAGTCTGA CAGAGTTCTC ATTACCCAAA TTTTACTTGC TGTTTGGCAT TAGCAAAGTC
AAGTCAGCTG CTGTCATTGC CTTCCGTTCC AGTTACCGGG ACAGGTCATT CTCTCCTGGC
TCCTATGAGA GAGAGACTTT TGCTTTGACA TCCTTGGAAA CAGTCACAGA AGCTTTGTTG
GAGATCATGG AGGCATGCAT GAGAGATATT CCAACGTGCA AGTGGCTGGA CCAGTGGACA
GAACTAGCTC AAAGATTTGC ATTCCAATAT AATCCATCCC TGCAACCAAG AGCTCTTGTT
GTCTTTGGGT GTATTAGCAA ACGAGTGTCT CATGGGCAGA TAAAGCAGAT AATCCGTATT
CTTAGCAAGG CACTTGAGAG TTGCTTAAAA GGACCTGACA CTTACAACAG TCAAGTTCTG
ATAGAAGCTA CAGTAATAGC ACTAACCAAA TTACAGCCAC TTCTTAATAA GGACTCGCCT
CTGCACAAAG CCCTCTTTTG GGTAGCTGTG GCTGTGCTGC AGCTTGATGA GGTCAACTTG
TATTCAGCAG GTACCGCACT TCTTGAACAA AACCTGCATA CTTTAGATAG TCTCCGTATA
TTCAATGACA AGAGTCCAGA GGAAGTATTT ATGGCAATCC GGAATCCTCT GGAGTGGCAC
TGCAAGCAAA TGGATCATTT TGTTGGACTC AATTTCAACT CTAACTTTAA CTTTGCATTG
GTTGGACACC TTTTAAAAGG GTACAGGCAT CCTTCACCTG CTATTGTTGC AAGAACAGTC
AGAATTTTAC ATACACTACT AACTCTGGTT AACAAACACA GAAATTGTGA CAAATTTGAA
GTGAATACAC AGAGCGTGGC CTACTTAGCA GCTTTACTTA CAGTGTCTGA AGAAGTTCGA
AGTCGCTGCA GCCTAAAACA TAGAAAGTCA CTTCTTCTTA CTGATATTTC AATGGAAAAT
GTTCCTATGG ATACATATCC CATTCATCAT GGTGACCCTT CCTATAGGAC ACTAAAGGAG
ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT GAAGGATACC TTGCAGCCAC CTATCCAACT
GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA TCCATGAGCC TGGACATGGG GCAACCTTCT
CAGGCCAACA CTAAGAAGTT GCTTGGAACA AGGAAAAGTT TTGATCACTT GATATCAGAC
ACAAAGGCTC CTAAAAGGCA AGAAATGGAA TCAGGGATCA CAACACCCCC CAAAATGAGG
AGAGTAGCAG AAACTGATTA TGAAATGGAA ACTCAGAGGA TTTCCTCATC ACAACAGCAC
CCACATTTAC GTAAAGTTTC AGTGTCTGAA TCAAATGTTC TCTTGGATGA AGAAGTACTT
ACTGATCCGA AGATCCAGGC GCTGCTTCTT ACTGTTCTAG CTACACTGGT AAAATATACC
ACAGATGAGT TTGATCAACG AATTCTTTAT GAATACTTAG CAGAGGCCAG TGTTGTGTTT
CCCAAAGTCT TTCCTGTTGT GCATAATTTG TTGGACTCTA AGATCAACAC CCTGTTATCA
TTGTGCCAAG ATCCAAATTT GTTAAATCCA ATCCATGGAA TTGTGCAGAG TGTGGTGTAC
CATGAAGAAT CCCCACCACA ATACCAAACA TCTTACCTGC AAAGTTTTGG TTTTAATGGC
TTGTGGCGGT TTGCAGGACC GTTTTCAAAG CAAACACAAA TTCCAGACTA TGCTGAGCTT
ATTGTTAAGT TTCTTGATGC CTTGATTGAC ACGTACCTGC CTGGAATTGA TGAAGAAACC
AGTGAAGAAT CCCTCCTGAC TCCCACATCT CCTTACCCTC CTGCACTGCA GAGCCAGCTT
AGTATCACTG CCAACCTTAA CCTTTCTAAT TCCATGACCT CACTTGCAAC TTCCCAGCAT
TCCCCAGATA CTGCAGCCAC TGAATTCCTT CCTGCTTCCC CGCTGATAAA CCTTTTAGCA
TTTTGGAGCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50433
Clone ID Related Accession (Same CDS sequence) XM_006721926 , XM_006721927
Accession Version XM_006721927.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8472bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neurofibromin isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
CGGAGGTTCA AAACTGGTCA AATCAATGGT GATTTGCTGA TATACCATGT CTTACTGACT
TTAAAGCCAT ATTATGCAAA GCCATATGAA ATTGTAGTGG ACCTTACCCA TACCGGGCCT
AGCAATCGCT TTAAAACAGA CTTTCTCTCT AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT
TACGACAACG TCTCCGCAGT CTATATCTAT AACTGTAACT CCTGGGTCAG GGAGTACACC
AAGTATCATG AGCGGCTGCT GACTGGCCTC AAAGGTAGCA AAAGGCTTGT TTTCATAGAC
TGTCCTGGGA AACTGGCTGA GCACATAGAG CATGAACAAC AGAAACTACC TGCTGCCACC
TTGGCTTTAG AAGAGGACCT GAAGGTATTC CACAATGCTC TCAAGCTAGC TCACAAAGAC
ACCAAAGTTT CTATTAAAGT TGGTTCTACT GCTGTCCAAG TAACTTCAGC AGAGCGAACA
AAAGTCCTAG GGCAATCAGT CTTTCTAAAT GACATTTATT ATGCTTCGGA AATTGAAGAA
ATCTGCCTAG TAGATGAGAA CCAGTTCACC TTAACCATTG CAAACCAGGG CACGCCGCTC
ACCTTCATGC ACCAGGAGTG TGAAGCCATT GTCCAGTCTA TCATTCATAT CCGGACCCGC
TGGGAACTGT CACAGCCCGA CTCTATCCCC CAACACACCA AGATTCGGCC AAAAGATGTC
CCTGGGACAC TGCTCAATAT CGCATTACTT AATTTAGGCA GTTCTGACCC GAGTTTACGG
TCAGCTGCCT ATAATCTTCT GTGTGCCTTA ACTTGTACCT TTAATTTAAA AATCGAGGGC
CAGTTACTAG AGACATCAGG TTTATGTATC CCTGCCAACA ACACCCTCTT TATTGTCTCT
ATTAGTAAGA CACTGGCAGC CAATGAGCCA CACCTCACGT TAGAATTTTT GGAAGAGTGT
ATTTCTGGAT TTAGCAAATC TAGTATTGAA TTGAAACACC TTTGTTTGGA ATACATGACT
CCATGGCTGT CAAATCTAGT TCGTTTTTGC AAGCATAATG ATGATGCCAA ACGACAAAGA
GTTACTGCTA TTCTTGACAA GCTGATAACA ATGACCATCA ATGAAAAACA GATGTACCCA
TCTATTCAAG CAAAAATATG GGGAAGCCTT GGGCAGATTA CAGATCTGCT TGATGTTGTA
CTAGACAGTT TCATCAAAAC CAGTGCAACA GGTGGCTTGG GATCAATAAA AGCTGAGGTG
ATGGCAGATA CTGCTGTAGC TTTGGCTTCT GGAAATGTGA AATTGGTTTC AAGCAAGGTT
ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACATGCTTAT CTCCAACTCC TACTTTAGAA
CAACATCTTA TGTGGGATGA TATTGCTATT TTAGCACGCT ACATGCTGAT GCTGTCCTTC
AACAATTCCC TTGATGTGGC AGCTCATCTT CCCTACCTCT TCCACGTTGT TACTTTCTTA
GTAGCCACAG GTCCGCTCTC CCTTAGAGCT TCCACACATG GACTGGTCAT TAATATCATT
CACTCTCTGT GTACTTGTTC ACAGCTTCAT TTTAGTGAAG AGACCAAGCA AGTTTTGAGA
CTCAGTCTGA CAGAGTTCTC ATTACCCAAA TTTTACTTGC TGTTTGGCAT TAGCAAAGTC
AAGTCAGCTG CTGTCATTGC CTTCCGTTCC AGTTACCGGG ACAGGTCATT CTCTCCTGGC
TCCTATGAGA GAGAGACTTT TGCTTTGACA TCCTTGGAAA CAGTCACAGA AGCTTTGTTG
GAGATCATGG AGGCATGCAT GAGAGATATT CCAACGTGCA AGTGGCTGGA CCAGTGGACA
GAACTAGCTC AAAGATTTGC ATTCCAATAT AATCCATCCC TGCAACCAAG AGCTCTTGTT
GTCTTTGGGT GTATTAGCAA ACGAGTGTCT CATGGGCAGA TAAAGCAGAT AATCCGTATT
CTTAGCAAGG CACTTGAGAG TTGCTTAAAA GGACCTGACA CTTACAACAG TCAAGTTCTG
ATAGAAGCTA CAGTAATAGC ACTAACCAAA TTACAGCCAC TTCTTAATAA GGACTCGCCT
CTGCACAAAG CCCTCTTTTG GGTAGCTGTG GCTGTGCTGC AGCTTGATGA GGTCAACTTG
TATTCAGCAG GTACCGCACT TCTTGAACAA AACCTGCATA CTTTAGATAG TCTCCGTATA
TTCAATGACA AGAGTCCAGA GGAAGTATTT ATGGCAATCC GGAATCCTCT GGAGTGGCAC
TGCAAGCAAA TGGATCATTT TGTTGGACTC AATTTCAACT CTAACTTTAA CTTTGCATTG
GTTGGACACC TTTTAAAAGG GTACAGGCAT CCTTCACCTG CTATTGTTGC AAGAACAGTC
AGAATTTTAC ATACACTACT AACTCTGGTT AACAAACACA GAAATTGTGA CAAATTTGAA
GTGAATACAC AGAGCGTGGC CTACTTAGCA GCTTTACTTA CAGTGTCTGA AGAAGTTCGA
AGTCGCTGCA GCCTAAAACA TAGAAAGTCA CTTCTTCTTA CTGATATTTC AATGGAAAAT
GTTCCTATGG ATACATATCC CATTCATCAT GGTGACCCTT CCTATAGGAC ACTAAAGGAG
ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT GAAGGATACC TTGCAGCCAC CTATCCAACT
GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA TCCATGAGCC TGGACATGGG GCAACCTTCT
CAGGCCAACA CTAAGAAGTT GCTTGGAACA AGGAAAAGTT TTGATCACTT GATATCAGAC
ACAAAGGCTC CTAAAAGGCA AGAAATGGAA TCAGGGATCA CAACACCCCC CAAAATGAGG
AGAGTAGCAG AAACTGATTA TGAAATGGAA ACTCAGAGGA TTTCCTCATC ACAACAGCAC
CCACATTTAC GTAAAGTTTC AGTGTCTGAA TCAAATGTTC TCTTGGATGA AGAAGTACTT
ACTGATCCGA AGATCCAGGC GCTGCTTCTT ACTGTTCTAG CTACACTGGT AAAATATACC
ACAGATGAGT TTGATCAACG AATTCTTTAT GAATACTTAG CAGAGGCCAG TGTTGTGTTT
CCCAAAGTCT TTCCTGTTGT GCATAATTTG TTGGACTCTA AGATCAACAC CCTGTTATCA
TTGTGCCAAG ATCCAAATTT GTTAAATCCA ATCCATGGAA TTGTGCAGAG TGTGGTGTAC
CATGAAGAAT CCCCACCACA ATACCAAACA TCTTACCTGC AAAGTTTTGG TTTTAATGGC
TTGTGGCGGT TTGCAGGACC GTTTTCAAAG CAAACACAAA TTCCAGACTA TGCTGAGCTT
ATTGTTAAGT TTCTTGATGC CTTGATTGAC ACGTACCTGC CTGGAATTGA TGAAGAAACC
AGTGAAGAAT CCCTCCTGAC TCCCACATCT CCTTACCCTC CTGCACTGCA GAGCCAGCTT
AGTATCACTG CCAACCTTAA CCTTTCTAAT TCCATGACCT CACTTGCAAC TTCCCAGCAT
TCCCCAGATA CTGCAGCCAC TGAATTCCTT CCTGCTTCCC CGCTGATAAA CCTTTTAGCA
TTTTGGAGCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006721990.1
CDS334..8805
Misc Feature(1)3922..5034(+)
Misc Feature(2)3955..5016(+)
Misc Feature(3)4069..4737(+)
Misc Feature(4)5104..5547(+)
Misc Feature(5)5173..5478(+)
Misc Feature(6)5377..5475(+)
Misc Feature(7)5545..5874(+)
Misc Feature(8)5545..5874(+)
Translation

Target ORF information:

RefSeq Version XM_006721927
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X14, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006721927

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
CGGAGGTTCA AAACTGGTCA AATCAATGGT GATTTGCTGA TATACCATGT CTTACTGACT
TTAAAGCCAT ATTATGCAAA GCCATATGAA ATTGTAGTGG ACCTTACCCA TACCGGGCCT
AGCAATCGCT TTAAAACAGA CTTTCTCTCT AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT
TACGACAACG TCTCCGCAGT CTATATCTAT AACTGTAACT CCTGGGTCAG GGAGTACACC
AAGTATCATG AGCGGCTGCT GACTGGCCTC AAAGGTAGCA AAAGGCTTGT TTTCATAGAC
TGTCCTGGGA AACTGGCTGA GCACATAGAG CATGAACAAC AGAAACTACC TGCTGCCACC
TTGGCTTTAG AAGAGGACCT GAAGGTATTC CACAATGCTC TCAAGCTAGC TCACAAAGAC
ACCAAAGTTT CTATTAAAGT TGGTTCTACT GCTGTCCAAG TAACTTCAGC AGAGCGAACA
AAAGTCCTAG GGCAATCAGT CTTTCTAAAT GACATTTATT ATGCTTCGGA AATTGAAGAA
ATCTGCCTAG TAGATGAGAA CCAGTTCACC TTAACCATTG CAAACCAGGG CACGCCGCTC
ACCTTCATGC ACCAGGAGTG TGAAGCCATT GTCCAGTCTA TCATTCATAT CCGGACCCGC
TGGGAACTGT CACAGCCCGA CTCTATCCCC CAACACACCA AGATTCGGCC AAAAGATGTC
CCTGGGACAC TGCTCAATAT CGCATTACTT AATTTAGGCA GTTCTGACCC GAGTTTACGG
TCAGCTGCCT ATAATCTTCT GTGTGCCTTA ACTTGTACCT TTAATTTAAA AATCGAGGGC
CAGTTACTAG AGACATCAGG TTTATGTATC CCTGCCAACA ACACCCTCTT TATTGTCTCT
ATTAGTAAGA CACTGGCAGC CAATGAGCCA CACCTCACGT TAGAATTTTT GGAAGAGTGT
ATTTCTGGAT TTAGCAAATC TAGTATTGAA TTGAAACACC TTTGTTTGGA ATACATGACT
CCATGGCTGT CAAATCTAGT TCGTTTTTGC AAGCATAATG ATGATGCCAA ACGACAAAGA
GTTACTGCTA TTCTTGACAA GCTGATAACA ATGACCATCA ATGAAAAACA GATGTACCCA
TCTATTCAAG CAAAAATATG GGGAAGCCTT GGGCAGATTA CAGATCTGCT TGATGTTGTA
CTAGACAGTT TCATCAAAAC CAGTGCAACA GGTGGCTTGG GATCAATAAA AGCTGAGGTG
ATGGCAGATA CTGCTGTAGC TTTGGCTTCT GGAAATGTGA AATTGGTTTC AAGCAAGGTT
ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACATGCTTAT CTCCAACTCC TACTTTAGAA
CAACATCTTA TGTGGGATGA TATTGCTATT TTAGCACGCT ACATGCTGAT GCTGTCCTTC
AACAATTCCC TTGATGTGGC AGCTCATCTT CCCTACCTCT TCCACGTTGT TACTTTCTTA
GTAGCCACAG GTCCGCTCTC CCTTAGAGCT TCCACACATG GACTGGTCAT TAATATCATT
CACTCTCTGT GTACTTGTTC ACAGCTTCAT TTTAGTGAAG AGACCAAGCA AGTTTTGAGA
CTCAGTCTGA CAGAGTTCTC ATTACCCAAA TTTTACTTGC TGTTTGGCAT TAGCAAAGTC
AAGTCAGCTG CTGTCATTGC CTTCCGTTCC AGTTACCGGG ACAGGTCATT CTCTCCTGGC
TCCTATGAGA GAGAGACTTT TGCTTTGACA TCCTTGGAAA CAGTCACAGA AGCTTTGTTG
GAGATCATGG AGGCATGCAT GAGAGATATT CCAACGTGCA AGTGGCTGGA CCAGTGGACA
GAACTAGCTC AAAGATTTGC ATTCCAATAT AATCCATCCC TGCAACCAAG AGCTCTTGTT
GTCTTTGGGT GTATTAGCAA ACGAGTGTCT CATGGGCAGA TAAAGCAGAT AATCCGTATT
CTTAGCAAGG CACTTGAGAG TTGCTTAAAA GGACCTGACA CTTACAACAG TCAAGTTCTG
ATAGAAGCTA CAGTAATAGC ACTAACCAAA TTACAGCCAC TTCTTAATAA GGACTCGCCT
CTGCACAAAG CCCTCTTTTG GGTAGCTGTG GCTGTGCTGC AGCTTGATGA GGTCAACTTG
TATTCAGCAG GTACCGCACT TCTTGAACAA AACCTGCATA CTTTAGATAG TCTCCGTATA
TTCAATGACA AGAGTCCAGA GGAAGTATTT ATGGCAATCC GGAATCCTCT GGAGTGGCAC
TGCAAGCAAA TGGATCATTT TGTTGGACTC AATTTCAACT CTAACTTTAA CTTTGCATTG
GTTGGACACC TTTTAAAAGG GTACAGGCAT CCTTCACCTG CTATTGTTGC AAGAACAGTC
AGAATTTTAC ATACACTACT AACTCTGGTT AACAAACACA GAAATTGTGA CAAATTTGAA
GTGAATACAC AGAGCGTGGC CTACTTAGCA GCTTTACTTA CAGTGTCTGA AGAAGTTCGA
AGTCGCTGCA GCCTAAAACA TAGAAAGTCA CTTCTTCTTA CTGATATTTC AATGGAAAAT
GTTCCTATGG ATACATATCC CATTCATCAT GGTGACCCTT CCTATAGGAC ACTAAAGGAG
ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT GAAGGATACC TTGCAGCCAC CTATCCAACT
GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA TCCATGAGCC TGGACATGGG GCAACCTTCT
CAGGCCAACA CTAAGAAGTT GCTTGGAACA AGGAAAAGTT TTGATCACTT GATATCAGAC
ACAAAGGCTC CTAAAAGGCA AGAAATGGAA TCAGGGATCA CAACACCCCC CAAAATGAGG
AGAGTAGCAG AAACTGATTA TGAAATGGAA ACTCAGAGGA TTTCCTCATC ACAACAGCAC
CCACATTTAC GTAAAGTTTC AGTGTCTGAA TCAAATGTTC TCTTGGATGA AGAAGTACTT
ACTGATCCGA AGATCCAGGC GCTGCTTCTT ACTGTTCTAG CTACACTGGT AAAATATACC
ACAGATGAGT TTGATCAACG AATTCTTTAT GAATACTTAG CAGAGGCCAG TGTTGTGTTT
CCCAAAGTCT TTCCTGTTGT GCATAATTTG TTGGACTCTA AGATCAACAC CCTGTTATCA
TTGTGCCAAG ATCCAAATTT GTTAAATCCA ATCCATGGAA TTGTGCAGAG TGTGGTGTAC
CATGAAGAAT CCCCACCACA ATACCAAACA TCTTACCTGC AAAGTTTTGG TTTTAATGGC
TTGTGGCGGT TTGCAGGACC GTTTTCAAAG CAAACACAAA TTCCAGACTA TGCTGAGCTT
ATTGTTAAGT TTCTTGATGC CTTGATTGAC ACGTACCTGC CTGGAATTGA TGAAGAAACC
AGTGAAGAAT CCCTCCTGAC TCCCACATCT CCTTACCCTC CTGCACTGCA GAGCCAGCTT
AGTATCACTG CCAACCTTAA CCTTTCTAAT TCCATGACCT CACTTGCAAC TTCCCAGCAT
TCCCCAGATA CTGCAGCCAC TGAATTCCTT CCTGCTTCCC CGCTGATAAA CCTTTTAGCA
TTTTGGAGCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50434
Clone ID Related Accession (Same CDS sequence) XM_006721928
Accession Version XM_006721928.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4899bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neurofibromin isoform X10
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578831002. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
CGGAGGATGG AGTCCCGAAG AGAAAATTAC ATGTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006721991.1
CDS334..5232
Misc Feature(1)3922..5034(+)
Misc Feature(2)3955..5016(+)
Misc Feature(3)4069..4737(+)
Translation

Target ORF information:

RefSeq Version XM_006721928
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens neurofibromin 1 (NF1), transcript variant X15, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006721928

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
CGGAGGATGG AGTCCCGAAG AGAAAATTAC ATGTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24060
Clone ID Related Accession (Same CDS sequence) NM_000267
Accession Version NM_000267.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8457bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neurofibromin isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D12625.1, M82814.1, CN415204.1, AC135724.9 and AK026658.1. This sequence is a reference standard in the RefSeqGene project. On Dec 3, 2009 this sequence version replaced gi:109826365. Transcript Variant: This variant (2) lacks an in-frame coding exon compared to transcript variant 1, resulting in a shorter isoform (2) missing an internal 21 aa segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## undergoes RNA editing :: PMID: 8602361, 11727199 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: M82814.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GCTCACAAAT GCTTTTTTAC
ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTG
CGGGAAATAT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
TCCTGTCACT TTCTCCTTTT TTACGGGGTA GGATGTGATA TTCCTTCTAG TGGAAATACC
AGTCAAATGT CCATGGATCA TGAAGAATTA CTACGTACTC CTGGAGCCTC TCTCCGGAAG
GGAAAAGGGA ACTCCTCTAT GGATAGTGCA GCAGGATGCA GCGGAACCCC CCCGATTTGC
CGACAAGCCC AGACCAAACT AGAAGTGGCC CTGTACATGT TTCTGTGGAA CCCTGACACT
GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC CGCCACCTCT GTGAGGAAGC AGATATCCGG
TGTGGGGTGG ATGAAGTGTC AGTGCATAAC CTCTTGCCCA ACTATAACAC ATTCATGGAG
TTTGCCTCTG TCAGCAATAT GATGTCAACA GGAAGAGCAG CACTTCAGAA AAGAGTGATG
GCACTGCTGA GGCGCATTGA GCATCCCACT GCAGGAAACA CTGAGGCTTG GGAAGATACA
CATGCAAAAT GGGAACAAGC AACAAAGCTA ATCCTTAACT ATCCAAAAGC CAAAATGGAA
GATGGCCAGG CTGCTGAAAG CCTTCACAAG ACCATTGTTA AGAGGCGAAT GTCCCATGTG
AGTGGAGGAG GATCCATAGA TTTGTCTGAC ACAGACTCCC TACAGGAATG GATCAACATG
ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG
GCAACCTATA GCCCACCCAT GGGTCCAGTC AGTGAACGTA AGGGTTCTAT GATTTCAGTG
ATGTCTTCAG AGGGAAACGC AGATACACCT GTCAGCAAAT TTATGGATCG GCTGTTGTCC
TTAATGGTGT GTAACCATGA GAAAGTGGGA CTTCAAATAC GGACCAATGT TAAGGATCTG
GTGGGTCTAG AATTGAGTCC TGCTCTGTAT CCAATGCTAT TTAACAAATT GAAGAATACC
ATCAGCAAGT TTTTTGACTC CCAAGGACAG GTTTTATTGA CTGATACCAA TACTCAATTT
GTAGAACAAA CCATAGCTAT AATGAAGAAC TTGCTAGATA ATCATACTGA AGGCAGCTCT
GAACATCTAG GGCAAGCTAG CATTGAAACA ATGATGTTAA ATCTGGTCAG GTATGTTCGT
GTGCTTGGGA ATATGGTCCA TGCAATTCAA ATAAAAACGA AACTGTGTCA ATTAGTTGAA
GTAATGATGG CAAGGAGAGA TGACCTCTCA TTTTGCCAAG AGATGAAATT TAGGAATAAG
ATGGTAGAAT ACCTGACAGA CTGGGTTATG GGAACATCAA ACCAAGCAGC AGATGATGAT
GTAAAATGTC TTACAAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTAGT TTCACTTCTA
GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA GATGGTGTGG AATTGATGGA AGCCAAATCA
CAGTTATTTC TTAAATACTT CACATTATTT ATGAACCTTT TGAATGACTG CAGTGAAGTT
GAAGATGAAA GTGCGCAAAC AGGTGGCAGG AAACGTGGCA TGTCTCGGAG GCTGGCATCA
CTGAGGCACT GTACGGTCCT TGCAATGTCA AACTTACTCA ATGCCAACGT AGACAGTGGT
CTCATGCACT CCATAGGCTT AGGTTACCAC AAGGATCTCC AGACAAGAGC TACATTTATG
GAAGTTCTGA CAAAAATCCT TCAACAAGGC ACAGAATTTG ACACACTTGC AGAAACAGTA
TTGGCTGATC GGTTTGAGAG ATTGGTGGAA CTGGTCACAA TGATGGGTGA TCAAGGAGAA
CTCCCTATAG CGATGGCTCT GGCCAATGTG GTTCCTTGTT CTCAGTGGGA TGAACTAGCT
CGAGTTCTGG TTACTCTGTT TGATTCTCGG CATTTACTCT ACCAACTGCT CTGGAACATG
TTTTCTAAAG AAGTAGAATT GGCAGACTCC ATGCAGACTC TCTTCCGAGG CAACAGCTTG
GCCAGTAAAA TAATGACATT CTGTTTCAAG GTATATGGTG CTACCTATCT ACAAAAACTC
CTGGATCCTT TATTACGAAT TGTGATCACA TCCTCTGATT GGCAACATGT TAGCTTTGAA
GTGGATCCTA CCAGGTTAGA ACCATCAGAG AGCCTTGAGG AAAACCAGCG GAACCTCCTT
CAGATGACTG AAAAGTTCTT CCATGCCATC ATCAGTTCCT CCTCAGAATT CCCCCCTCAA
CTTCGAAGTG TGTGCCACTG TTTATACCAG GTGGTTAGCC AGCGTTTCCC TCAGAACAGC
ATCGGTGCAG TAGGAAGTGC CATGTTCCTC AGATTTATCA ATCCTGCCAT TGTCTCACCG
TATGAAGCAG GGATTTTAGA TAAAAAGCCA CCACCTAGAA TCGAAAGGGG CTTGAAGTTA
ATGTCAAAGA TACTTCAGAG TATTGCCAAT CATGTTCTCT TCACAAAAGA AGAACATATG
CGGCCTTTCA ATGATTTTGT GAAAAGCAAC TTTGATGCAG CACGCAGGTT TTTCCTTGAT
ATAGCATCTG ATTGTCCTAC AAGTGATGCA GTAAATCATA GTCTTTCCTT CATAAGTGAC
GGCAATGTGC TTGCTTTACA TCGTCTACTC TGGAACAATC AGGAGAAAAT TGGGCAGTAT
CTTTCCAGCA ACAGGGATCA TAAAGCTGTT GGAAGACGAC CTTTTGATAA GATGGCAACA
CTTCTTGCAT ACCTGGGTCC TCCAGAGCAC AAACCTGTGG CAGATACACA CTGGTCCAGC
CTTAACCTTA CCAGTTCAAA GTTTGAGGAA TTTATGACTA GGCATCAGGT ACATGAAAAA
GAAGAATTCA AGGCTTTGAA AACGTTAAGT ATTTTCTACC AAGCTGGGAC TTCCAAAGCT
GGGAATCCTA TTTTTTATTA TGTTGCACGG AGGTTCAAAA CTGGTCAAAT CAATGGTGAT
TTGCTGATAT ACCATGTCTT ACTGACTTTA AAGCCATATT ATGCAAAGCC ATATGAAATT
GTAGTGGACC TTACCCATAC CGGGCCTAGC AATCGCTTTA AAACAGACTT TCTCTCTAAG
TGGTTTGTTG TTTTTCCTGG CTTTGCTTAC GACAACGTCT CCGCAGTCTA TATCTATAAC
TGTAACTCCT GGGTCAGGGA GTACACCAAG TATCATGAGC GGCTGCTGAC TGGCCTCAAA
GGTAGCAAAA GGCTTGTTTT CATAGACTGT CCTGGGAAAC TGGCTGAGCA CATAGAGCAT
GAACAACAGA AACTACCTGC TGCCACCTTG GCTTTAGAAG AGGACCTGAA GGTATTCCAC
AATGCTCTCA AGCTAGCTCA CAAAGACACC AAAGTTTCTA TTAAAGTTGG TTCTACTGCT
GTCCAAGTAA CTTCAGCAGA GCGAACAAAA GTCCTAGGGC AATCAGTCTT TCTAAATGAC
ATTTATTATG CTTCGGAAAT TGAAGAAATC TGCCTAGTAG ATGAGAACCA GTTCACCTTA
ACCATTGCAA ACCAGGGCAC GCCGCTCACC TTCATGCACC AGGAGTGTGA AGCCATTGTC
CAGTCTATCA TTCATATCCG GACCCGCTGG GAACTGTCAC AGCCCGACTC TATCCCCCAA
CACACCAAGA TTCGGCCAAA AGATGTCCCT GGGACACTGC TCAATATCGC ATTACTTAAT
TTAGGCAGTT CTGACCCGAG TTTACGGTCA GCTGCCTATA ATCTTCTGTG TGCCTTAACT
TGTACCTTTA ATTTAAAAAT CGAGGGCCAG TTACTAGAGA CATCAGGTTT ATGTATCCCT
GCCAACAACA CCCTCTTTAT TGTCTCTATT AGTAAGACAC TGGCAGCCAA TGAGCCACAC
CTCACGTTAG AATTTTTGGA AGAGTGTATT TCTGGATTTA GCAAATCTAG TATTGAATTG
AAACACCTTT GTTTGGAATA CATGACTCCA TGGCTGTCAA ATCTAGTTCG TTTTTGCAAG
CATAATGATG ATGCCAAACG ACAAAGAGTT ACTGCTATTC TTGACAAGCT GATAACAATG
ACCATCAATG AAAAACAGAT GTACCCATCT ATTCAAGCAA AAATATGGGG AAGCCTTGGG
CAGATTACAG ATCTGCTTGA TGTTGTACTA GACAGTTTCA TCAAAACCAG TGCAACAGGT
GGCTTGGGAT CAATAAAAGC TGAGGTGATG GCAGATACTG CTGTAGCTTT GGCTTCTGGA
AATGTGAAAT TGGTTTCAAG CAAGGTTATT GGAAGGATGT GCAAAATAAT TGACAAGACA
TGCTTATCTC CAACTCCTAC TTTAGAACAA CATCTTATGT GGGATGATAT TGCTATTTTA
GCACGCTACA TGCTGATGCT GTCCTTCAAC AATTCCCTTG ATGTGGCAGC TCATCTTCCC
TACCTCTTCC ACGTTGTTAC TTTCTTAGTA GCCACAGGTC CGCTCTCCCT TAGAGCTTCC
ACACATGGAC TGGTCATTAA TATCATTCAC TCTCTGTGTA CTTGTTCACA GCTTCATTTT
AGTGAAGAGA CCAAGCAAGT TTTGAGACTC AGTCTGACAG AGTTCTCATT ACCCAAATTT
TACTTGCTGT TTGGCATTAG CAAAGTCAAG TCAGCTGCTG TCATTGCCTT CCGTTCCAGT
TACCGGGACA GGTCATTCTC TCCTGGCTCC TATGAGAGAG AGACTTTTGC TTTGACATCC
TTGGAAACAG TCACAGAAGC TTTGTTGGAG ATCATGGAGG CATGCATGAG AGATATTCCA
ACGTGCAAGT GGCTGGACCA GTGGACAGAA CTAGCTCAAA GATTTGCATT CCAATATAAT
CCATCCCTGC AACCAAGAGC TCTTGTTGTC TTTGGGTGTA TTAGCAAACG AGTGTCTCAT
GGGCAGATAA AGCAGATAAT CCGTATTCTT AGCAAGGCAC TTGAGAGTTG CTTAAAAGGA
CCTGACACTT ACAACAGTCA AGTTCTGATA GAAGCTACAG TAATAGCACT AACCAAATTA
CAGCCACTTC TTAATAAGGA CTCGCCTCTG CACAAAGCCC TCTTTTGGGT AGCTGTGGCT
GTGCTGCAGC TTGATGAGGT CAACTTGTAT TCAGCAGGTA CCGCACTTCT TGAACAAAAC
CTGCATACTT TAGATAGTCT CCGTATATTC AATGACAAGA GTCCAGAGGA AGTATTTATG
GCAATCCGGA ATCCTCTGGA GTGGCACTGC AAGCAAATGG ATCATTTTGT TGGACTCAAT
TTCAACTCTA ACTTTAACTT TGCATTGGTT GGACACCTTT TAAAAGGGTA CAGGCATCCT
TCACCTGCTA TTGTTGCAAG AACAGTCAGA ATTTTACATA CACTACTAAC TCTGGTTAAC
AAACACAGAA ATTGTGACAA ATTTGAAGTG AATACACAGA GCGTGGCCTA CTTAGCAGCT
TTACTTACAG TGTCTGAAGA AGTTCGAAGT CGCTGCAGCC TAAAACATAG AAAGTCACTT
CTTCTTACTG ATATTTCAAT GGAAAATGTT CCTATGGATA CATATCCCAT TCATCATGGT
GACCCTTCCT ATAGGACACT AAAGGAGACT CAGCCATGGT CCTCTCCCAA AGGTTCTGAA
GGATACCTTG CAGCCACCTA TCCAACTGTC GGCCAGACCA GTCCCCGAGC CAGGAAATCC
ATGAGCCTGG ACATGGGGCA ACCTTCTCAG GCCAACACTA AGAAGTTGCT TGGAACAAGG
AAAAGTTTTG ATCACTTGAT ATCAGACACA AAGGCTCCTA AAAGGCAAGA AATGGAATCA
GGGATCACAA CACCCCCCAA AATGAGGAGA GTAGCAGAAA CTGATTATGA AATGGAAACT
CAGAGGATTT CCTCATCACA ACAGCACCCA CATTTACGTA AAGTTTCAGT GTCTGAATCA
AATGTTCTCT TGGATGAAGA AGTACTTACT GATCCGAAGA TCCAGGCGCT GCTTCTTACT
GTTCTAGCTA CACTGGTAAA ATATACCACA GATGAGTTTG ATCAACGAAT TCTTTATGAA
TACTTAGCAG AGGCCAGTGT TGTGTTTCCC AAAGTCTTTC CTGTTGTGCA TAATTTGTTG
GACTCTAAGA TCAACACCCT GTTATCATTG TGCCAAGATC CAAATTTGTT AAATCCAATC
CATGGAATTG TGCAGAGTGT GGTGTACCAT GAAGAATCCC CACCACAATA CCAAACATCT
TACCTGCAAA GTTTTGGTTT TAATGGCTTG TGGCGGTTTG CAGGACCGTT TTCAAAGCAA
ACACAAATTC CAGACTATGC TGAGCTTATT GTTAAGTTTC TTGATGCCTT GATTGACACG
TACCTGCCTG GAATTGATGA AGAAACCAGT GAAGAATCCC TCCTGACTCC CACATCTCCT
TACCCTCCTG CACTGCAGAG CCAGCTTAGT ATCACTGCCA ACCTTAACCT TTCTAATTCC
ATGACCTCAC TTGCAACTTC CCAGCATTCC CCAGGAATCG ACAAGGAGAA CGTTGAACTC
TCCCCTACCA CTGGCCACTG TAACAGTGGA CGAACTCGCC ACGGATCCGC AAGCCAAGTG
CAGAAGCAAA GAAGCGCTGG CAGTTTCAAA CGTAATAGCA TTAAGAAGAT CGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000258.1
CDS384..8840
Misc Feature(1)225..227(+)
Misc Feature(2)2973..2975(+)
Misc Feature(3)2973..2975(+)
Misc Feature(4)3009..3011(+)
Misc Feature(5)3942..4991(+)
Misc Feature(6)3975..4973(+)
Misc Feature(7)4089..4694(+)
Misc Feature(8)5058..5831(+)
Misc Feature(9)5061..5504(+)
Misc Feature(10)5130..5435(+)
Misc Feature(11)5334..5432(+)
Misc Feature(12)5502..5831(+)
Misc Feature(13)5502..5831(+)
Misc Feature(14)6882..6884(+)
Misc Feature(15)6882..6884(+)
Misc Feature(16)7746..7748(+)
Misc Feature(17)7863..7865(+)
Misc Feature(18)7863..7865(+)
Misc Feature(19)7863..7865(+)
Misc Feature(20)7881..7883(+)
Misc Feature(21)7947..7949(+)
Misc Feature(22)8769..8771(+)
Misc Feature(23)8805..8807(+)
Exon (1)1..443
Gene:NF1
Gene Synonym:
Exon (2)444..587
Gene:NF1
Gene Synonym:
Exon (3)588..671
Gene:NF1
Gene Synonym:
Exon (4)672..862
Gene:NF1
Gene Synonym:
Exon (5)863..969
Gene:NF1
Gene Synonym:
Exon (6)970..1037
Gene:NF1
Gene Synonym:
Exon (7)1038..1113
Gene:NF1
Gene Synonym:
Exon (8)1114..1271
Gene:NF1
Gene Synonym:
Exon (9)1272..1445
Gene:NF1
Gene Synonym:
Exon (10)1446..1568
Gene:NF1
Gene Synonym:
Exon (11)1569..1643
Gene:NF1
Gene Synonym:
Exon (12)1644..1775
Gene:NF1
Gene Synonym:
Exon (13)1776..1910
Gene:NF1
Gene Synonym:
Exon (14)1911..2024
Gene:NF1
Gene Synonym:
Exon (15)2025..2104
Gene:NF1
Gene Synonym:
Exon (16)2105..2228
Gene:NF1
Gene Synonym:
Exon (17)2229..2384
Gene:NF1
Gene Synonym:
Exon (18)2385..2634
Gene:NF1
Gene Synonym:
Exon (19)2635..2708
Gene:NF1
Gene Synonym:
Exon (20)2709..2792
Gene:NF1
Gene Synonym:
Exon (21)2793..3233
Gene:NF1
Gene Synonym:
Exon (22)3234..3373
Gene:NF1
Gene Synonym:
Exon (23)3374..3496
Gene:NF1
Gene Synonym:
Exon (24)3497..3580
Gene:NF1
Gene Synonym:
Exon (25)3581..3697
Gene:NF1
Gene Synonym:
Exon (26)3698..3879
Gene:NF1
Gene Synonym:
Exon (27)3880..4091
Gene:NF1
Gene Synonym:
Exon (28)4092..4253
Gene:NF1
Gene Synonym:
Exon (29)4254..4357
Gene:NF1
Gene Synonym:
Exon (30)4358..4493
Gene:NF1
Gene Synonym:
Exon (31)4494..4652
Gene:NF1
Gene Synonym:
Exon (32)4653..4750
Gene:NF1
Gene Synonym:
Exon (33)4751..4897
Gene:NF1
Gene Synonym:
Exon (34)4898..5044
Gene:NF1
Gene Synonym:
Exon (35)5045..5155
Gene:NF1
Gene Synonym:
Exon (36)5156..5588
Gene:NF1
Gene Synonym:
Exon (37)5589..5929
Gene:NF1
Gene Synonym:
Exon (38)5930..6132
Gene:NF1
Gene Synonym:
Exon (39)6133..6326
Gene:NF1
Gene Synonym:
Exon (40)6327..6467
Gene:NF1
Gene Synonym:
Exon (41)6468..6747
Gene:NF1
Gene Synonym:
Exon (42)6748..6962
Gene:NF1
Gene Synonym:
Exon (43)6963..7024
Gene:NF1
Gene Synonym:
Exon (44)7025..7139
Gene:NF1
Gene Synonym:
Exon (45)7140..7241
Gene:NF1
Gene Synonym:
Exon (46)7242..7382
Gene:NF1
Gene Synonym:
Exon (47)7383..7509
Gene:NF1
Gene Synonym:
Exon (48)7510..7641
Gene:NF1
Gene Synonym:
Exon (49)7642..7777
Gene:NF1
Gene Synonym:
Exon (50)7778..7935
Gene:NF1
Gene Synonym:
Exon (51)7936..8058
Gene:NF1
Gene Synonym:
Exon (52)8059..8189
Gene:NF1
Gene Synonym:
Exon (53)8190..8290
Gene:NF1
Gene Synonym:
Exon (54)8291..8433
Gene:NF1
Gene Synonym:
Exon (55)8434..8480
Gene:NF1
Gene Synonym:
Exon (56)8481..8697
Gene:NF1
Gene Synonym:
Exon (57)8698..12362
Gene:NF1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000267
Organism Homo sapiens (human)
Definition Homo sapiens neurofibromin 1 (NF1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000267

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GCTCACAAAT GCTTTTTTAC
ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTG
CGGGAAATAT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
TCCTGTCACT TTCTCCTTTT TTACGGGGTA GGATGTGATA TTCCTTCTAG TGGAAATACC
AGTCAAATGT CCATGGATCA TGAAGAATTA CTACGTACTC CTGGAGCCTC TCTCCGGAAG
GGAAAAGGGA ACTCCTCTAT GGATAGTGCA GCAGGATGCA GCGGAACCCC CCCGATTTGC
CGACAAGCCC AGACCAAACT AGAAGTGGCC CTGTACATGT TTCTGTGGAA CCCTGACACT
GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC CGCCACCTCT GTGAGGAAGC AGATATCCGG
TGTGGGGTGG ATGAAGTGTC AGTGCATAAC CTCTTGCCCA ACTATAACAC ATTCATGGAG
TTTGCCTCTG TCAGCAATAT GATGTCAACA GGAAGAGCAG CACTTCAGAA AAGAGTGATG
GCACTGCTGA GGCGCATTGA GCATCCCACT GCAGGAAACA CTGAGGCTTG GGAAGATACA
CATGCAAAAT GGGAACAAGC AACAAAGCTA ATCCTTAACT ATCCAAAAGC CAAAATGGAA
GATGGCCAGG CTGCTGAAAG CCTTCACAAG ACCATTGTTA AGAGGCGAAT GTCCCATGTG
AGTGGAGGAG GATCCATAGA TTTGTCTGAC ACAGACTCCC TACAGGAATG GATCAACATG
ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG
GCAACCTATA GCCCACCCAT GGGTCCAGTC AGTGAACGTA AGGGTTCTAT GATTTCAGTG
ATGTCTTCAG AGGGAAACGC AGATACACCT GTCAGCAAAT TTATGGATCG GCTGTTGTCC
TTAATGGTGT GTAACCATGA GAAAGTGGGA CTTCAAATAC GGACCAATGT TAAGGATCTG
GTGGGTCTAG AATTGAGTCC TGCTCTGTAT CCAATGCTAT TTAACAAATT GAAGAATACC
ATCAGCAAGT TTTTTGACTC CCAAGGACAG GTTTTATTGA CTGATACCAA TACTCAATTT
GTAGAACAAA CCATAGCTAT AATGAAGAAC TTGCTAGATA ATCATACTGA AGGCAGCTCT
GAACATCTAG GGCAAGCTAG CATTGAAACA ATGATGTTAA ATCTGGTCAG GTATGTTCGT
GTGCTTGGGA ATATGGTCCA TGCAATTCAA ATAAAAACGA AACTGTGTCA ATTAGTTGAA
GTAATGATGG CAAGGAGAGA TGACCTCTCA TTTTGCCAAG AGATGAAATT TAGGAATAAG
ATGGTAGAAT ACCTGACAGA CTGGGTTATG GGAACATCAA ACCAAGCAGC AGATGATGAT
GTAAAATGTC TTACAAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTAGT TTCACTTCTA
GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA GATGGTGTGG AATTGATGGA AGCCAAATCA
CAGTTATTTC TTAAATACTT CACATTATTT ATGAACCTTT TGAATGACTG CAGTGAAGTT
GAAGATGAAA GTGCGCAAAC AGGTGGCAGG AAACGTGGCA TGTCTCGGAG GCTGGCATCA
CTGAGGCACT GTACGGTCCT TGCAATGTCA AACTTACTCA ATGCCAACGT AGACAGTGGT
CTCATGCACT CCATAGGCTT AGGTTACCAC AAGGATCTCC AGACAAGAGC TACATTTATG
GAAGTTCTGA CAAAAATCCT TCAACAAGGC ACAGAATTTG ACACACTTGC AGAAACAGTA
TTGGCTGATC GGTTTGAGAG ATTGGTGGAA CTGGTCACAA TGATGGGTGA TCAAGGAGAA
CTCCCTATAG CGATGGCTCT GGCCAATGTG GTTCCTTGTT CTCAGTGGGA TGAACTAGCT
CGAGTTCTGG TTACTCTGTT TGATTCTCGG CATTTACTCT ACCAACTGCT CTGGAACATG
TTTTCTAAAG AAGTAGAATT GGCAGACTCC ATGCAGACTC TCTTCCGAGG CAACAGCTTG
GCCAGTAAAA TAATGACATT CTGTTTCAAG GTATATGGTG CTACCTATCT ACAAAAACTC
CTGGATCCTT TATTACGAAT TGTGATCACA TCCTCTGATT GGCAACATGT TAGCTTTGAA
GTGGATCCTA CCAGGTTAGA ACCATCAGAG AGCCTTGAGG AAAACCAGCG GAACCTCCTT
CAGATGACTG AAAAGTTCTT CCATGCCATC ATCAGTTCCT CCTCAGAATT CCCCCCTCAA
CTTCGAAGTG TGTGCCACTG TTTATACCAG GTGGTTAGCC AGCGTTTCCC TCAGAACAGC
ATCGGTGCAG TAGGAAGTGC CATGTTCCTC AGATTTATCA ATCCTGCCAT TGTCTCACCG
TATGAAGCAG GGATTTTAGA TAAAAAGCCA CCACCTAGAA TCGAAAGGGG CTTGAAGTTA
ATGTCAAAGA TACTTCAGAG TATTGCCAAT CATGTTCTCT TCACAAAAGA AGAACATATG
CGGCCTTTCA ATGATTTTGT GAAAAGCAAC TTTGATGCAG CACGCAGGTT TTTCCTTGAT
ATAGCATCTG ATTGTCCTAC AAGTGATGCA GTAAATCATA GTCTTTCCTT CATAAGTGAC
GGCAATGTGC TTGCTTTACA TCGTCTACTC TGGAACAATC AGGAGAAAAT TGGGCAGTAT
CTTTCCAGCA ACAGGGATCA TAAAGCTGTT GGAAGACGAC CTTTTGATAA GATGGCAACA
CTTCTTGCAT ACCTGGGTCC TCCAGAGCAC AAACCTGTGG CAGATACACA CTGGTCCAGC
CTTAACCTTA CCAGTTCAAA GTTTGAGGAA TTTATGACTA GGCATCAGGT ACATGAAAAA
GAAGAATTCA AGGCTTTGAA AACGTTAAGT ATTTTCTACC AAGCTGGGAC TTCCAAAGCT
GGGAATCCTA TTTTTTATTA TGTTGCACGG AGGTTCAAAA CTGGTCAAAT CAATGGTGAT
TTGCTGATAT ACCATGTCTT ACTGACTTTA AAGCCATATT ATGCAAAGCC ATATGAAATT
GTAGTGGACC TTACCCATAC CGGGCCTAGC AATCGCTTTA AAACAGACTT TCTCTCTAAG
TGGTTTGTTG TTTTTCCTGG CTTTGCTTAC GACAACGTCT CCGCAGTCTA TATCTATAAC
TGTAACTCCT GGGTCAGGGA GTACACCAAG TATCATGAGC GGCTGCTGAC TGGCCTCAAA
GGTAGCAAAA GGCTTGTTTT CATAGACTGT CCTGGGAAAC TGGCTGAGCA CATAGAGCAT
GAACAACAGA AACTACCTGC TGCCACCTTG GCTTTAGAAG AGGACCTGAA GGTATTCCAC
AATGCTCTCA AGCTAGCTCA CAAAGACACC AAAGTTTCTA TTAAAGTTGG TTCTACTGCT
GTCCAAGTAA CTTCAGCAGA GCGAACAAAA GTCCTAGGGC AATCAGTCTT TCTAAATGAC
ATTTATTATG CTTCGGAAAT TGAAGAAATC TGCCTAGTAG ATGAGAACCA GTTCACCTTA
ACCATTGCAA ACCAGGGCAC GCCGCTCACC TTCATGCACC AGGAGTGTGA AGCCATTGTC
CAGTCTATCA TTCATATCCG GACCCGCTGG GAACTGTCAC AGCCCGACTC TATCCCCCAA
CACACCAAGA TTCGGCCAAA AGATGTCCCT GGGACACTGC TCAATATCGC ATTACTTAAT
TTAGGCAGTT CTGACCCGAG TTTACGGTCA GCTGCCTATA ATCTTCTGTG TGCCTTAACT
TGTACCTTTA ATTTAAAAAT CGAGGGCCAG TTACTAGAGA CATCAGGTTT ATGTATCCCT
GCCAACAACA CCCTCTTTAT TGTCTCTATT AGTAAGACAC TGGCAGCCAA TGAGCCACAC
CTCACGTTAG AATTTTTGGA AGAGTGTATT TCTGGATTTA GCAAATCTAG TATTGAATTG
AAACACCTTT GTTTGGAATA CATGACTCCA TGGCTGTCAA ATCTAGTTCG TTTTTGCAAG
CATAATGATG ATGCCAAACG ACAAAGAGTT ACTGCTATTC TTGACAAGCT GATAACAATG
ACCATCAATG AAAAACAGAT GTACCCATCT ATTCAAGCAA AAATATGGGG AAGCCTTGGG
CAGATTACAG ATCTGCTTGA TGTTGTACTA GACAGTTTCA TCAAAACCAG TGCAACAGGT
GGCTTGGGAT CAATAAAAGC TGAGGTGATG GCAGATACTG CTGTAGCTTT GGCTTCTGGA
AATGTGAAAT TGGTTTCAAG CAAGGTTATT GGAAGGATGT GCAAAATAAT TGACAAGACA
TGCTTATCTC CAACTCCTAC TTTAGAACAA CATCTTATGT GGGATGATAT TGCTATTTTA
GCACGCTACA TGCTGATGCT GTCCTTCAAC AATTCCCTTG ATGTGGCAGC TCATCTTCCC
TACCTCTTCC ACGTTGTTAC TTTCTTAGTA GCCACAGGTC CGCTCTCCCT TAGAGCTTCC
ACACATGGAC TGGTCATTAA TATCATTCAC TCTCTGTGTA CTTGTTCACA GCTTCATTTT
AGTGAAGAGA CCAAGCAAGT TTTGAGACTC AGTCTGACAG AGTTCTCATT ACCCAAATTT
TACTTGCTGT TTGGCATTAG CAAAGTCAAG TCAGCTGCTG TCATTGCCTT CCGTTCCAGT
TACCGGGACA GGTCATTCTC TCCTGGCTCC TATGAGAGAG AGACTTTTGC TTTGACATCC
TTGGAAACAG TCACAGAAGC TTTGTTGGAG ATCATGGAGG CATGCATGAG AGATATTCCA
ACGTGCAAGT GGCTGGACCA GTGGACAGAA CTAGCTCAAA GATTTGCATT CCAATATAAT
CCATCCCTGC AACCAAGAGC TCTTGTTGTC TTTGGGTGTA TTAGCAAACG AGTGTCTCAT
GGGCAGATAA AGCAGATAAT CCGTATTCTT AGCAAGGCAC TTGAGAGTTG CTTAAAAGGA
CCTGACACTT ACAACAGTCA AGTTCTGATA GAAGCTACAG TAATAGCACT AACCAAATTA
CAGCCACTTC TTAATAAGGA CTCGCCTCTG CACAAAGCCC TCTTTTGGGT AGCTGTGGCT
GTGCTGCAGC TTGATGAGGT CAACTTGTAT TCAGCAGGTA CCGCACTTCT TGAACAAAAC
CTGCATACTT TAGATAGTCT CCGTATATTC AATGACAAGA GTCCAGAGGA AGTATTTATG
GCAATCCGGA ATCCTCTGGA GTGGCACTGC AAGCAAATGG ATCATTTTGT TGGACTCAAT
TTCAACTCTA ACTTTAACTT TGCATTGGTT GGACACCTTT TAAAAGGGTA CAGGCATCCT
TCACCTGCTA TTGTTGCAAG AACAGTCAGA ATTTTACATA CACTACTAAC TCTGGTTAAC
AAACACAGAA ATTGTGACAA ATTTGAAGTG AATACACAGA GCGTGGCCTA CTTAGCAGCT
TTACTTACAG TGTCTGAAGA AGTTCGAAGT CGCTGCAGCC TAAAACATAG AAAGTCACTT
CTTCTTACTG ATATTTCAAT GGAAAATGTT CCTATGGATA CATATCCCAT TCATCATGGT
GACCCTTCCT ATAGGACACT AAAGGAGACT CAGCCATGGT CCTCTCCCAA AGGTTCTGAA
GGATACCTTG CAGCCACCTA TCCAACTGTC GGCCAGACCA GTCCCCGAGC CAGGAAATCC
ATGAGCCTGG ACATGGGGCA ACCTTCTCAG GCCAACACTA AGAAGTTGCT TGGAACAAGG
AAAAGTTTTG ATCACTTGAT ATCAGACACA AAGGCTCCTA AAAGGCAAGA AATGGAATCA
GGGATCACAA CACCCCCCAA AATGAGGAGA GTAGCAGAAA CTGATTATGA AATGGAAACT
CAGAGGATTT CCTCATCACA ACAGCACCCA CATTTACGTA AAGTTTCAGT GTCTGAATCA
AATGTTCTCT TGGATGAAGA AGTACTTACT GATCCGAAGA TCCAGGCGCT GCTTCTTACT
GTTCTAGCTA CACTGGTAAA ATATACCACA GATGAGTTTG ATCAACGAAT TCTTTATGAA
TACTTAGCAG AGGCCAGTGT TGTGTTTCCC AAAGTCTTTC CTGTTGTGCA TAATTTGTTG
GACTCTAAGA TCAACACCCT GTTATCATTG TGCCAAGATC CAAATTTGTT AAATCCAATC
CATGGAATTG TGCAGAGTGT GGTGTACCAT GAAGAATCCC CACCACAATA CCAAACATCT
TACCTGCAAA GTTTTGGTTT TAATGGCTTG TGGCGGTTTG CAGGACCGTT TTCAAAGCAA
ACACAAATTC CAGACTATGC TGAGCTTATT GTTAAGTTTC TTGATGCCTT GATTGACACG
TACCTGCCTG GAATTGATGA AGAAACCAGT GAAGAATCCC TCCTGACTCC CACATCTCCT
TACCCTCCTG CACTGCAGAG CCAGCTTAGT ATCACTGCCA ACCTTAACCT TTCTAATTCC
ATGACCTCAC TTGCAACTTC CCAGCATTCC CCAGGAATCG ACAAGGAGAA CGTTGAACTC
TCCCCTACCA CTGGCCACTG TAACAGTGGA CGAACTCGCC ACGGATCCGC AAGCCAAGTG
CAGAAGCAAA GAAGCGCTGG CAGTTTCAAA CGTAATAGCA TTAAGAAGAT CGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23641
Clone ID Related Accession (Same CDS sequence) NM_001042492
Accession Version NM_001042492.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8520bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neurofibromin isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D12625.1, M89914.1, BX391799.2, BP271334.1, AC135724.9 and AK026658.1. This sequence is a reference standard in the RefSeqGene project. On Dec 3, 2009 this sequence version replaced gi:109826563. Transcript Variant: This variant (1), with an additional in-frame coding exon, represents the longest transcript and encodes the longest isoform (1). Studies suggest preferential C->U RNA editing of transcripts containing this exon. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## undergoes RNA editing :: PMID: 8602361, 11727199 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: M89914.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GCTCACAAAT GCTTTTTTAC
ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTG
CGGGAAATAT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
TCCTGTCACT TTCTCCTTTT TTACGGGGTA GGATGTGATA TTCCTTCTAG TGGAAATACC
AGTCAAATGT CCATGGATCA TGAAGAATTA CTACGTACTC CTGGAGCCTC TCTCCGGAAG
GGAAAAGGGA ACTCCTCTAT GGATAGTGCA GCAGGATGCA GCGGAACCCC CCCGATTTGC
CGACAAGCCC AGACCAAACT AGAAGTGGCC CTGTACATGT TTCTGTGGAA CCCTGACACT
GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC CGCCACCTCT GTGAGGAAGC AGATATCCGG
TGTGGGGTGG ATGAAGTGTC AGTGCATAAC CTCTTGCCCA ACTATAACAC ATTCATGGAG
TTTGCCTCTG TCAGCAATAT GATGTCAACA GGAAGAGCAG CACTTCAGAA AAGAGTGATG
GCACTGCTGA GGCGCATTGA GCATCCCACT GCAGGAAACA CTGAGGCTTG GGAAGATACA
CATGCAAAAT GGGAACAAGC AACAAAGCTA ATCCTTAACT ATCCAAAAGC CAAAATGGAA
GATGGCCAGG CTGCTGAAAG CCTTCACAAG ACCATTGTTA AGAGGCGAAT GTCCCATGTG
AGTGGAGGAG GATCCATAGA TTTGTCTGAC ACAGACTCCC TACAGGAATG GATCAACATG
ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG
GCAACCTATA GCCCACCCAT GGGTCCAGTC AGTGAACGTA AGGGTTCTAT GATTTCAGTG
ATGTCTTCAG AGGGAAACGC AGATACACCT GTCAGCAAAT TTATGGATCG GCTGTTGTCC
TTAATGGTGT GTAACCATGA GAAAGTGGGA CTTCAAATAC GGACCAATGT TAAGGATCTG
GTGGGTCTAG AATTGAGTCC TGCTCTGTAT CCAATGCTAT TTAACAAATT GAAGAATACC
ATCAGCAAGT TTTTTGACTC CCAAGGACAG GTTTTATTGA CTGATACCAA TACTCAATTT
GTAGAACAAA CCATAGCTAT AATGAAGAAC TTGCTAGATA ATCATACTGA AGGCAGCTCT
GAACATCTAG GGCAAGCTAG CATTGAAACA ATGATGTTAA ATCTGGTCAG GTATGTTCGT
GTGCTTGGGA ATATGGTCCA TGCAATTCAA ATAAAAACGA AACTGTGTCA ATTAGTTGAA
GTAATGATGG CAAGGAGAGA TGACCTCTCA TTTTGCCAAG AGATGAAATT TAGGAATAAG
ATGGTAGAAT ACCTGACAGA CTGGGTTATG GGAACATCAA ACCAAGCAGC AGATGATGAT
GTAAAATGTC TTACAAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTAGT TTCACTTCTA
GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA GATGGTGTGG AATTGATGGA AGCCAAATCA
CAGTTATTTC TTAAATACTT CACATTATTT ATGAACCTTT TGAATGACTG CAGTGAAGTT
GAAGATGAAA GTGCGCAAAC AGGTGGCAGG AAACGTGGCA TGTCTCGGAG GCTGGCATCA
CTGAGGCACT GTACGGTCCT TGCAATGTCA AACTTACTCA ATGCCAACGT AGACAGTGGT
CTCATGCACT CCATAGGCTT AGGTTACCAC AAGGATCTCC AGACAAGAGC TACATTTATG
GAAGTTCTGA CAAAAATCCT TCAACAAGGC ACAGAATTTG ACACACTTGC AGAAACAGTA
TTGGCTGATC GGTTTGAGAG ATTGGTGGAA CTGGTCACAA TGATGGGTGA TCAAGGAGAA
CTCCCTATAG CGATGGCTCT GGCCAATGTG GTTCCTTGTT CTCAGTGGGA TGAACTAGCT
CGAGTTCTGG TTACTCTGTT TGATTCTCGG CATTTACTCT ACCAACTGCT CTGGAACATG
TTTTCTAAAG AAGTAGAATT GGCAGACTCC ATGCAGACTC TCTTCCGAGG CAACAGCTTG
GCCAGTAAAA TAATGACATT CTGTTTCAAG GTATATGGTG CTACCTATCT ACAAAAACTC
CTGGATCCTT TATTACGAAT TGTGATCACA TCCTCTGATT GGCAACATGT TAGCTTTGAA
GTGGATCCTA CCAGGTTAGA ACCATCAGAG AGCCTTGAGG AAAACCAGCG GAACCTCCTT
CAGATGACTG AAAAGTTCTT CCATGCCATC ATCAGTTCCT CCTCAGAATT CCCCCCTCAA
CTTCGAAGTG TGTGCCACTG TTTATACCAG GCAACTTGCC ACTCCCTACT GAATAAAGCT
ACAGTAAAAG AAAAAAAGGA AAACAAAAAA TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC
AGCATCGGTG CAGTAGGAAG TGCCATGTTC CTCAGATTTA TCAATCCTGC CATTGTCTCA
CCGTATGAAG CAGGGATTTT AGATAAAAAG CCACCACCTA GAATCGAAAG GGGCTTGAAG
TTAATGTCAA AGATACTTCA GAGTATTGCC AATCATGTTC TCTTCACAAA AGAAGAACAT
ATGCGGCCTT TCAATGATTT TGTGAAAAGC AACTTTGATG CAGCACGCAG GTTTTTCCTT
GATATAGCAT CTGATTGTCC TACAAGTGAT GCAGTAAATC ATAGTCTTTC CTTCATAAGT
GACGGCAATG TGCTTGCTTT ACATCGTCTA CTCTGGAACA ATCAGGAGAA AATTGGGCAG
TATCTTTCCA GCAACAGGGA TCATAAAGCT GTTGGAAGAC GACCTTTTGA TAAGATGGCA
ACACTTCTTG CATACCTGGG TCCTCCAGAG CACAAACCTG TGGCAGATAC ACACTGGTCC
AGCCTTAACC TTACCAGTTC AAAGTTTGAG GAATTTATGA CTAGGCATCA GGTACATGAA
AAAGAAGAAT TCAAGGCTTT GAAAACGTTA AGTATTTTCT ACCAAGCTGG GACTTCCAAA
GCTGGGAATC CTATTTTTTA TTATGTTGCA CGGAGGTTCA AAACTGGTCA AATCAATGGT
GATTTGCTGA TATACCATGT CTTACTGACT TTAAAGCCAT ATTATGCAAA GCCATATGAA
ATTGTAGTGG ACCTTACCCA TACCGGGCCT AGCAATCGCT TTAAAACAGA CTTTCTCTCT
AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT TACGACAACG TCTCCGCAGT CTATATCTAT
AACTGTAACT CCTGGGTCAG GGAGTACACC AAGTATCATG AGCGGCTGCT GACTGGCCTC
AAAGGTAGCA AAAGGCTTGT TTTCATAGAC TGTCCTGGGA AACTGGCTGA GCACATAGAG
CATGAACAAC AGAAACTACC TGCTGCCACC TTGGCTTTAG AAGAGGACCT GAAGGTATTC
CACAATGCTC TCAAGCTAGC TCACAAAGAC ACCAAAGTTT CTATTAAAGT TGGTTCTACT
GCTGTCCAAG TAACTTCAGC AGAGCGAACA AAAGTCCTAG GGCAATCAGT CTTTCTAAAT
GACATTTATT ATGCTTCGGA AATTGAAGAA ATCTGCCTAG TAGATGAGAA CCAGTTCACC
TTAACCATTG CAAACCAGGG CACGCCGCTC ACCTTCATGC ACCAGGAGTG TGAAGCCATT
GTCCAGTCTA TCATTCATAT CCGGACCCGC TGGGAACTGT CACAGCCCGA CTCTATCCCC
CAACACACCA AGATTCGGCC AAAAGATGTC CCTGGGACAC TGCTCAATAT CGCATTACTT
AATTTAGGCA GTTCTGACCC GAGTTTACGG TCAGCTGCCT ATAATCTTCT GTGTGCCTTA
ACTTGTACCT TTAATTTAAA AATCGAGGGC CAGTTACTAG AGACATCAGG TTTATGTATC
CCTGCCAACA ACACCCTCTT TATTGTCTCT ATTAGTAAGA CACTGGCAGC CAATGAGCCA
CACCTCACGT TAGAATTTTT GGAAGAGTGT ATTTCTGGAT TTAGCAAATC TAGTATTGAA
TTGAAACACC TTTGTTTGGA ATACATGACT CCATGGCTGT CAAATCTAGT TCGTTTTTGC
AAGCATAATG ATGATGCCAA ACGACAAAGA GTTACTGCTA TTCTTGACAA GCTGATAACA
ATGACCATCA ATGAAAAACA GATGTACCCA TCTATTCAAG CAAAAATATG GGGAAGCCTT
GGGCAGATTA CAGATCTGCT TGATGTTGTA CTAGACAGTT TCATCAAAAC CAGTGCAACA
GGTGGCTTGG GATCAATAAA AGCTGAGGTG ATGGCAGATA CTGCTGTAGC TTTGGCTTCT
GGAAATGTGA AATTGGTTTC AAGCAAGGTT ATTGGAAGGA TGTGCAAAAT AATTGACAAG
ACATGCTTAT CTCCAACTCC TACTTTAGAA CAACATCTTA TGTGGGATGA TATTGCTATT
TTAGCACGCT ACATGCTGAT GCTGTCCTTC AACAATTCCC TTGATGTGGC AGCTCATCTT
CCCTACCTCT TCCACGTTGT TACTTTCTTA GTAGCCACAG GTCCGCTCTC CCTTAGAGCT
TCCACACATG GACTGGTCAT TAATATCATT CACTCTCTGT GTACTTGTTC ACAGCTTCAT
TTTAGTGAAG AGACCAAGCA AGTTTTGAGA CTCAGTCTGA CAGAGTTCTC ATTACCCAAA
TTTTACTTGC TGTTTGGCAT TAGCAAAGTC AAGTCAGCTG CTGTCATTGC CTTCCGTTCC
AGTTACCGGG ACAGGTCATT CTCTCCTGGC TCCTATGAGA GAGAGACTTT TGCTTTGACA
TCCTTGGAAA CAGTCACAGA AGCTTTGTTG GAGATCATGG AGGCATGCAT GAGAGATATT
CCAACGTGCA AGTGGCTGGA CCAGTGGACA GAACTAGCTC AAAGATTTGC ATTCCAATAT
AATCCATCCC TGCAACCAAG AGCTCTTGTT GTCTTTGGGT GTATTAGCAA ACGAGTGTCT
CATGGGCAGA TAAAGCAGAT AATCCGTATT CTTAGCAAGG CACTTGAGAG TTGCTTAAAA
GGACCTGACA CTTACAACAG TCAAGTTCTG ATAGAAGCTA CAGTAATAGC ACTAACCAAA
TTACAGCCAC TTCTTAATAA GGACTCGCCT CTGCACAAAG CCCTCTTTTG GGTAGCTGTG
GCTGTGCTGC AGCTTGATGA GGTCAACTTG TATTCAGCAG GTACCGCACT TCTTGAACAA
AACCTGCATA CTTTAGATAG TCTCCGTATA TTCAATGACA AGAGTCCAGA GGAAGTATTT
ATGGCAATCC GGAATCCTCT GGAGTGGCAC TGCAAGCAAA TGGATCATTT TGTTGGACTC
AATTTCAACT CTAACTTTAA CTTTGCATTG GTTGGACACC TTTTAAAAGG GTACAGGCAT
CCTTCACCTG CTATTGTTGC AAGAACAGTC AGAATTTTAC ATACACTACT AACTCTGGTT
AACAAACACA GAAATTGTGA CAAATTTGAA GTGAATACAC AGAGCGTGGC CTACTTAGCA
GCTTTACTTA CAGTGTCTGA AGAAGTTCGA AGTCGCTGCA GCCTAAAACA TAGAAAGTCA
CTTCTTCTTA CTGATATTTC AATGGAAAAT GTTCCTATGG ATACATATCC CATTCATCAT
GGTGACCCTT CCTATAGGAC ACTAAAGGAG ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT
GAAGGATACC TTGCAGCCAC CTATCCAACT GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA
TCCATGAGCC TGGACATGGG GCAACCTTCT CAGGCCAACA CTAAGAAGTT GCTTGGAACA
AGGAAAAGTT TTGATCACTT GATATCAGAC ACAAAGGCTC CTAAAAGGCA AGAAATGGAA
TCAGGGATCA CAACACCCCC CAAAATGAGG AGAGTAGCAG AAACTGATTA TGAAATGGAA
ACTCAGAGGA TTTCCTCATC ACAACAGCAC CCACATTTAC GTAAAGTTTC AGTGTCTGAA
TCAAATGTTC TCTTGGATGA AGAAGTACTT ACTGATCCGA AGATCCAGGC GCTGCTTCTT
ACTGTTCTAG CTACACTGGT AAAATATACC ACAGATGAGT TTGATCAACG AATTCTTTAT
GAATACTTAG CAGAGGCCAG TGTTGTGTTT CCCAAAGTCT TTCCTGTTGT GCATAATTTG
TTGGACTCTA AGATCAACAC CCTGTTATCA TTGTGCCAAG ATCCAAATTT GTTAAATCCA
ATCCATGGAA TTGTGCAGAG TGTGGTGTAC CATGAAGAAT CCCCACCACA ATACCAAACA
TCTTACCTGC AAAGTTTTGG TTTTAATGGC TTGTGGCGGT TTGCAGGACC GTTTTCAAAG
CAAACACAAA TTCCAGACTA TGCTGAGCTT ATTGTTAAGT TTCTTGATGC CTTGATTGAC
ACGTACCTGC CTGGAATTGA TGAAGAAACC AGTGAAGAAT CCCTCCTGAC TCCCACATCT
CCTTACCCTC CTGCACTGCA GAGCCAGCTT AGTATCACTG CCAACCTTAA CCTTTCTAAT
TCCATGACCT CACTTGCAAC TTCCCAGCAT TCCCCAGGAA TCGACAAGGA GAACGTTGAA
CTCTCCCCTA CCACTGGCCA CTGTAACAGT GGACGAACTC GCCACGGATC CGCAAGCCAA
GTGCAGAAGC AAAGAAGCGC TGGCAGTTTC AAACGTAATA GCATTAAGAA GATCGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001035957.1
CDS384..8903
Misc Feature(1)225..227(+)
Misc Feature(2)2973..2975(+)
Misc Feature(3)3009..3011(+)
Misc Feature(4)3942..5054(+)
Misc Feature(5)3975..5036(+)
Misc Feature(6)4089..4757(+)
Misc Feature(7)5121..5894(+)
Misc Feature(8)5124..5567(+)
Misc Feature(9)5193..5498(+)
Misc Feature(10)5397..5495(+)
Misc Feature(11)5565..5894(+)
Misc Feature(12)5565..5894(+)
Misc Feature(13)6945..6947(+)
Misc Feature(14)7809..7811(+)
Misc Feature(15)7926..7928(+)
Misc Feature(16)7926..7928(+)
Misc Feature(17)7944..7946(+)
Misc Feature(18)8010..8012(+)
Misc Feature(19)8832..8834(+)
Exon (1)1..443
Gene:NF1
Gene Synonym:
Exon (2)444..587
Gene:NF1
Gene Synonym:
Exon (3)588..671
Gene:NF1
Gene Synonym:
Exon (4)672..862
Gene:NF1
Gene Synonym:
Exon (5)863..969
Gene:NF1
Gene Synonym:
Exon (6)970..1037
Gene:NF1
Gene Synonym:
Exon (7)1038..1113
Gene:NF1
Gene Synonym:
Exon (8)1114..1271
Gene:NF1
Gene Synonym:
Exon (9)1272..1445
Gene:NF1
Gene Synonym:
Exon (10)1446..1568
Gene:NF1
Gene Synonym:
Exon (11)1569..1643
Gene:NF1
Gene Synonym:
Exon (12)1644..1775
Gene:NF1
Gene Synonym:
Exon (13)1776..1910
Gene:NF1
Gene Synonym:
Exon (14)1911..2024
Gene:NF1
Gene Synonym:
Exon (15)2025..2104
Gene:NF1
Gene Synonym:
Exon (16)2105..2228
Gene:NF1
Gene Synonym:
Exon (17)2229..2384
Gene:NF1
Gene Synonym:
Exon (18)2385..2634
Gene:NF1
Gene Synonym:
Exon (19)2635..2708
Gene:NF1
Gene Synonym:
Exon (20)2709..2792
Gene:NF1
Gene Synonym:
Exon (21)2793..3233
Gene:NF1
Gene Synonym:
Exon (22)3234..3373
Gene:NF1
Gene Synonym:
Exon (23)3374..3496
Gene:NF1
Gene Synonym:
Exon (24)3497..3580
Gene:NF1
Gene Synonym:
Exon (25)3581..3697
Gene:NF1
Gene Synonym:
Exon (26)3698..3879
Gene:NF1
Gene Synonym:
Exon (27)3880..4091
Gene:NF1
Gene Synonym:
Exon (28)4092..4253
Gene:NF1
Gene Synonym:
Exon (29)4254..4357
Gene:NF1
Gene Synonym:
Exon (30)4358..4493
Gene:NF1
Gene Synonym:
Exon (31)4494..4556
Gene:NF1
Gene Synonym:
Exon (32)4557..4715
Gene:NF1
Gene Synonym:
Exon (33)4716..4813
Gene:NF1
Gene Synonym:
Exon (34)4814..4960
Gene:NF1
Gene Synonym:
Exon (35)4961..5107
Gene:NF1
Gene Synonym:
Exon (36)5108..5218
Gene:NF1
Gene Synonym:
Exon (37)5219..5651
Gene:NF1
Gene Synonym:
Exon (38)5652..5992
Gene:NF1
Gene Synonym:
Exon (39)5993..6195
Gene:NF1
Gene Synonym:
Exon (40)6196..6389
Gene:NF1
Gene Synonym:
Exon (41)6390..6530
Gene:NF1
Gene Synonym:
Exon (42)6531..6810
Gene:NF1
Gene Synonym:
Exon (43)6811..7025
Gene:NF1
Gene Synonym:
Exon (44)7026..7087
Gene:NF1
Gene Synonym:
Exon (45)7088..7202
Gene:NF1
Gene Synonym:
Exon (46)7203..7304
Gene:NF1
Gene Synonym:
Exon (47)7305..7445
Gene:NF1
Gene Synonym:
Exon (48)7446..7572
Gene:NF1
Gene Synonym:
Exon (49)7573..7704
Gene:NF1
Gene Synonym:
Exon (50)7705..7840
Gene:NF1
Gene Synonym:
Exon (51)7841..7998
Gene:NF1
Gene Synonym:
Exon (52)7999..8121
Gene:NF1
Gene Synonym:
Exon (53)8122..8252
Gene:NF1
Gene Synonym:
Exon (54)8253..8353
Gene:NF1
Gene Synonym:
Exon (55)8354..8496
Gene:NF1
Gene Synonym:
Exon (56)8497..8543
Gene:NF1
Gene Synonym:
Exon (57)8544..8760
Gene:NF1
Gene Synonym:
Exon (58)8761..12425
Gene:NF1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001042492
Organism Homo sapiens (human)
Definition Homo sapiens neurofibromin 1 (NF1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001042492

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GCTCACAAAT GCTTTTTTAC
ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTG
CGGGAAATAT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
TCCTGTCACT TTCTCCTTTT TTACGGGGTA GGATGTGATA TTCCTTCTAG TGGAAATACC
AGTCAAATGT CCATGGATCA TGAAGAATTA CTACGTACTC CTGGAGCCTC TCTCCGGAAG
GGAAAAGGGA ACTCCTCTAT GGATAGTGCA GCAGGATGCA GCGGAACCCC CCCGATTTGC
CGACAAGCCC AGACCAAACT AGAAGTGGCC CTGTACATGT TTCTGTGGAA CCCTGACACT
GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC CGCCACCTCT GTGAGGAAGC AGATATCCGG
TGTGGGGTGG ATGAAGTGTC AGTGCATAAC CTCTTGCCCA ACTATAACAC ATTCATGGAG
TTTGCCTCTG TCAGCAATAT GATGTCAACA GGAAGAGCAG CACTTCAGAA AAGAGTGATG
GCACTGCTGA GGCGCATTGA GCATCCCACT GCAGGAAACA CTGAGGCTTG GGAAGATACA
CATGCAAAAT GGGAACAAGC AACAAAGCTA ATCCTTAACT ATCCAAAAGC CAAAATGGAA
GATGGCCAGG CTGCTGAAAG CCTTCACAAG ACCATTGTTA AGAGGCGAAT GTCCCATGTG
AGTGGAGGAG GATCCATAGA TTTGTCTGAC ACAGACTCCC TACAGGAATG GATCAACATG
ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG
GCAACCTATA GCCCACCCAT GGGTCCAGTC AGTGAACGTA AGGGTTCTAT GATTTCAGTG
ATGTCTTCAG AGGGAAACGC AGATACACCT GTCAGCAAAT TTATGGATCG GCTGTTGTCC
TTAATGGTGT GTAACCATGA GAAAGTGGGA CTTCAAATAC GGACCAATGT TAAGGATCTG
GTGGGTCTAG AATTGAGTCC TGCTCTGTAT CCAATGCTAT TTAACAAATT GAAGAATACC
ATCAGCAAGT TTTTTGACTC CCAAGGACAG GTTTTATTGA CTGATACCAA TACTCAATTT
GTAGAACAAA CCATAGCTAT AATGAAGAAC TTGCTAGATA ATCATACTGA AGGCAGCTCT
GAACATCTAG GGCAAGCTAG CATTGAAACA ATGATGTTAA ATCTGGTCAG GTATGTTCGT
GTGCTTGGGA ATATGGTCCA TGCAATTCAA ATAAAAACGA AACTGTGTCA ATTAGTTGAA
GTAATGATGG CAAGGAGAGA TGACCTCTCA TTTTGCCAAG AGATGAAATT TAGGAATAAG
ATGGTAGAAT ACCTGACAGA CTGGGTTATG GGAACATCAA ACCAAGCAGC AGATGATGAT
GTAAAATGTC TTACAAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTAGT TTCACTTCTA
GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA GATGGTGTGG AATTGATGGA AGCCAAATCA
CAGTTATTTC TTAAATACTT CACATTATTT ATGAACCTTT TGAATGACTG CAGTGAAGTT
GAAGATGAAA GTGCGCAAAC AGGTGGCAGG AAACGTGGCA TGTCTCGGAG GCTGGCATCA
CTGAGGCACT GTACGGTCCT TGCAATGTCA AACTTACTCA ATGCCAACGT AGACAGTGGT
CTCATGCACT CCATAGGCTT AGGTTACCAC AAGGATCTCC AGACAAGAGC TACATTTATG
GAAGTTCTGA CAAAAATCCT TCAACAAGGC ACAGAATTTG ACACACTTGC AGAAACAGTA
TTGGCTGATC GGTTTGAGAG ATTGGTGGAA CTGGTCACAA TGATGGGTGA TCAAGGAGAA
CTCCCTATAG CGATGGCTCT GGCCAATGTG GTTCCTTGTT CTCAGTGGGA TGAACTAGCT
CGAGTTCTGG TTACTCTGTT TGATTCTCGG CATTTACTCT ACCAACTGCT CTGGAACATG
TTTTCTAAAG AAGTAGAATT GGCAGACTCC ATGCAGACTC TCTTCCGAGG CAACAGCTTG
GCCAGTAAAA TAATGACATT CTGTTTCAAG GTATATGGTG CTACCTATCT ACAAAAACTC
CTGGATCCTT TATTACGAAT TGTGATCACA TCCTCTGATT GGCAACATGT TAGCTTTGAA
GTGGATCCTA CCAGGTTAGA ACCATCAGAG AGCCTTGAGG AAAACCAGCG GAACCTCCTT
CAGATGACTG AAAAGTTCTT CCATGCCATC ATCAGTTCCT CCTCAGAATT CCCCCCTCAA
CTTCGAAGTG TGTGCCACTG TTTATACCAG GCAACTTGCC ACTCCCTACT GAATAAAGCT
ACAGTAAAAG AAAAAAAGGA AAACAAAAAA TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC
AGCATCGGTG CAGTAGGAAG TGCCATGTTC CTCAGATTTA TCAATCCTGC CATTGTCTCA
CCGTATGAAG CAGGGATTTT AGATAAAAAG CCACCACCTA GAATCGAAAG GGGCTTGAAG
TTAATGTCAA AGATACTTCA GAGTATTGCC AATCATGTTC TCTTCACAAA AGAAGAACAT
ATGCGGCCTT TCAATGATTT TGTGAAAAGC AACTTTGATG CAGCACGCAG GTTTTTCCTT
GATATAGCAT CTGATTGTCC TACAAGTGAT GCAGTAAATC ATAGTCTTTC CTTCATAAGT
GACGGCAATG TGCTTGCTTT ACATCGTCTA CTCTGGAACA ATCAGGAGAA AATTGGGCAG
TATCTTTCCA GCAACAGGGA TCATAAAGCT GTTGGAAGAC GACCTTTTGA TAAGATGGCA
ACACTTCTTG CATACCTGGG TCCTCCAGAG CACAAACCTG TGGCAGATAC ACACTGGTCC
AGCCTTAACC TTACCAGTTC AAAGTTTGAG GAATTTATGA CTAGGCATCA GGTACATGAA
AAAGAAGAAT TCAAGGCTTT GAAAACGTTA AGTATTTTCT ACCAAGCTGG GACTTCCAAA
GCTGGGAATC CTATTTTTTA TTATGTTGCA CGGAGGTTCA AAACTGGTCA AATCAATGGT
GATTTGCTGA TATACCATGT CTTACTGACT TTAAAGCCAT ATTATGCAAA GCCATATGAA
ATTGTAGTGG ACCTTACCCA TACCGGGCCT AGCAATCGCT TTAAAACAGA CTTTCTCTCT
AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT TACGACAACG TCTCCGCAGT CTATATCTAT
AACTGTAACT CCTGGGTCAG GGAGTACACC AAGTATCATG AGCGGCTGCT GACTGGCCTC
AAAGGTAGCA AAAGGCTTGT TTTCATAGAC TGTCCTGGGA AACTGGCTGA GCACATAGAG
CATGAACAAC AGAAACTACC TGCTGCCACC TTGGCTTTAG AAGAGGACCT GAAGGTATTC
CACAATGCTC TCAAGCTAGC TCACAAAGAC ACCAAAGTTT CTATTAAAGT TGGTTCTACT
GCTGTCCAAG TAACTTCAGC AGAGCGAACA AAAGTCCTAG GGCAATCAGT CTTTCTAAAT
GACATTTATT ATGCTTCGGA AATTGAAGAA ATCTGCCTAG TAGATGAGAA CCAGTTCACC
TTAACCATTG CAAACCAGGG CACGCCGCTC ACCTTCATGC ACCAGGAGTG TGAAGCCATT
GTCCAGTCTA TCATTCATAT CCGGACCCGC TGGGAACTGT CACAGCCCGA CTCTATCCCC
CAACACACCA AGATTCGGCC AAAAGATGTC CCTGGGACAC TGCTCAATAT CGCATTACTT
AATTTAGGCA GTTCTGACCC GAGTTTACGG TCAGCTGCCT ATAATCTTCT GTGTGCCTTA
ACTTGTACCT TTAATTTAAA AATCGAGGGC CAGTTACTAG AGACATCAGG TTTATGTATC
CCTGCCAACA ACACCCTCTT TATTGTCTCT ATTAGTAAGA CACTGGCAGC CAATGAGCCA
CACCTCACGT TAGAATTTTT GGAAGAGTGT ATTTCTGGAT TTAGCAAATC TAGTATTGAA
TTGAAACACC TTTGTTTGGA ATACATGACT CCATGGCTGT CAAATCTAGT TCGTTTTTGC
AAGCATAATG ATGATGCCAA ACGACAAAGA GTTACTGCTA TTCTTGACAA GCTGATAACA
ATGACCATCA ATGAAAAACA GATGTACCCA TCTATTCAAG CAAAAATATG GGGAAGCCTT
GGGCAGATTA CAGATCTGCT TGATGTTGTA CTAGACAGTT TCATCAAAAC CAGTGCAACA
GGTGGCTTGG GATCAATAAA AGCTGAGGTG ATGGCAGATA CTGCTGTAGC TTTGGCTTCT
GGAAATGTGA AATTGGTTTC AAGCAAGGTT ATTGGAAGGA TGTGCAAAAT AATTGACAAG
ACATGCTTAT CTCCAACTCC TACTTTAGAA CAACATCTTA TGTGGGATGA TATTGCTATT
TTAGCACGCT ACATGCTGAT GCTGTCCTTC AACAATTCCC TTGATGTGGC AGCTCATCTT
CCCTACCTCT TCCACGTTGT TACTTTCTTA GTAGCCACAG GTCCGCTCTC CCTTAGAGCT
TCCACACATG GACTGGTCAT TAATATCATT CACTCTCTGT GTACTTGTTC ACAGCTTCAT
TTTAGTGAAG AGACCAAGCA AGTTTTGAGA CTCAGTCTGA CAGAGTTCTC ATTACCCAAA
TTTTACTTGC TGTTTGGCAT TAGCAAAGTC AAGTCAGCTG CTGTCATTGC CTTCCGTTCC
AGTTACCGGG ACAGGTCATT CTCTCCTGGC TCCTATGAGA GAGAGACTTT TGCTTTGACA
TCCTTGGAAA CAGTCACAGA AGCTTTGTTG GAGATCATGG AGGCATGCAT GAGAGATATT
CCAACGTGCA AGTGGCTGGA CCAGTGGACA GAACTAGCTC AAAGATTTGC ATTCCAATAT
AATCCATCCC TGCAACCAAG AGCTCTTGTT GTCTTTGGGT GTATTAGCAA ACGAGTGTCT
CATGGGCAGA TAAAGCAGAT AATCCGTATT CTTAGCAAGG CACTTGAGAG TTGCTTAAAA
GGACCTGACA CTTACAACAG TCAAGTTCTG ATAGAAGCTA CAGTAATAGC ACTAACCAAA
TTACAGCCAC TTCTTAATAA GGACTCGCCT CTGCACAAAG CCCTCTTTTG GGTAGCTGTG
GCTGTGCTGC AGCTTGATGA GGTCAACTTG TATTCAGCAG GTACCGCACT TCTTGAACAA
AACCTGCATA CTTTAGATAG TCTCCGTATA TTCAATGACA AGAGTCCAGA GGAAGTATTT
ATGGCAATCC GGAATCCTCT GGAGTGGCAC TGCAAGCAAA TGGATCATTT TGTTGGACTC
AATTTCAACT CTAACTTTAA CTTTGCATTG GTTGGACACC TTTTAAAAGG GTACAGGCAT
CCTTCACCTG CTATTGTTGC AAGAACAGTC AGAATTTTAC ATACACTACT AACTCTGGTT
AACAAACACA GAAATTGTGA CAAATTTGAA GTGAATACAC AGAGCGTGGC CTACTTAGCA
GCTTTACTTA CAGTGTCTGA AGAAGTTCGA AGTCGCTGCA GCCTAAAACA TAGAAAGTCA
CTTCTTCTTA CTGATATTTC AATGGAAAAT GTTCCTATGG ATACATATCC CATTCATCAT
GGTGACCCTT CCTATAGGAC ACTAAAGGAG ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT
GAAGGATACC TTGCAGCCAC CTATCCAACT GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA
TCCATGAGCC TGGACATGGG GCAACCTTCT CAGGCCAACA CTAAGAAGTT GCTTGGAACA
AGGAAAAGTT TTGATCACTT GATATCAGAC ACAAAGGCTC CTAAAAGGCA AGAAATGGAA
TCAGGGATCA CAACACCCCC CAAAATGAGG AGAGTAGCAG AAACTGATTA TGAAATGGAA
ACTCAGAGGA TTTCCTCATC ACAACAGCAC CCACATTTAC GTAAAGTTTC AGTGTCTGAA
TCAAATGTTC TCTTGGATGA AGAAGTACTT ACTGATCCGA AGATCCAGGC GCTGCTTCTT
ACTGTTCTAG CTACACTGGT AAAATATACC ACAGATGAGT TTGATCAACG AATTCTTTAT
GAATACTTAG CAGAGGCCAG TGTTGTGTTT CCCAAAGTCT TTCCTGTTGT GCATAATTTG
TTGGACTCTA AGATCAACAC CCTGTTATCA TTGTGCCAAG ATCCAAATTT GTTAAATCCA
ATCCATGGAA TTGTGCAGAG TGTGGTGTAC CATGAAGAAT CCCCACCACA ATACCAAACA
TCTTACCTGC AAAGTTTTGG TTTTAATGGC TTGTGGCGGT TTGCAGGACC GTTTTCAAAG
CAAACACAAA TTCCAGACTA TGCTGAGCTT ATTGTTAAGT TTCTTGATGC CTTGATTGAC
ACGTACCTGC CTGGAATTGA TGAAGAAACC AGTGAAGAAT CCCTCCTGAC TCCCACATCT
CCTTACCCTC CTGCACTGCA GAGCCAGCTT AGTATCACTG CCAACCTTAA CCTTTCTAAT
TCCATGACCT CACTTGCAAC TTCCCAGCAT TCCCCAGGAA TCGACAAGGA GAACGTTGAA
CTCTCCCCTA CCACTGGCCA CTGTAACAGT GGACGAACTC GCCACGGATC CGCAAGCCAA
GTGCAGAAGC AAAGAAGCGC TGGCAGTTTC AAACGTAATA GCATTAAGAA GATCGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26980
Clone ID Related Accession (Same CDS sequence) NM_001128147
Accession Version NM_001128147.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1782bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neurofibromin isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D12625.1, M82814.1 and D42072.1. On Dec 3, 2009 this sequence version replaced gi:189458803. Transcript Variant: This variant (3) lacks multiple 3' exons and has an alternate 3' end, as compared to variant 1. The resulting isoform (3) has a much shorter and different C-terminus, and lacks ras-GTPase activating domain and SEC14 domain, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## undergoes RNA editing :: PMID: 8602361, 11727199 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: D42072.1, AK289936.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GGTATATGTA CTTTTATTTT
TTAAATTCAA CTTTTAAATT TTATTTTGTA TTTTTGTCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001121619.1
CDS384..2165
Misc Feature(1)225..227(+)
Exon (1)1..443
Gene:NF1
Gene Synonym:
Exon (2)444..587
Gene:NF1
Gene Synonym:
Exon (3)588..671
Gene:NF1
Gene Synonym:
Exon (4)672..862
Gene:NF1
Gene Synonym:
Exon (5)863..969
Gene:NF1
Gene Synonym:
Exon (6)970..1037
Gene:NF1
Gene Synonym:
Exon (7)1038..1113
Gene:NF1
Gene Synonym:
Exon (8)1114..1271
Gene:NF1
Gene Synonym:
Exon (9)1272..1445
Gene:NF1
Gene Synonym:
Exon (10)1446..1568
Gene:NF1
Gene Synonym:
Exon (11)1569..1643
Gene:NF1
Gene Synonym:
Exon (12)1644..1775
Gene:NF1
Gene Synonym:
Exon (13)1776..1910
Gene:NF1
Gene Synonym:
Exon (14)1911..2024
Gene:NF1
Gene Synonym:
Exon (15)2025..2939
Gene:NF1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001128147
Organism Homo sapiens (human)
Definition Homo sapiens neurofibromin 1 (NF1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001128147

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GGTATATGTA CTTTTATTTT
TTAAATTCAA CTTTTAAATT TTATTTTGTA TTTTTGTCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
*
*
*
*