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NF2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol NF2
Entrez Gene ID 4771
Full Name neurofibromin 2 (merlin)
Synonyms ACN, BANF, SCH
General protein information
Preferred Names
merlin
Names
merlin
schwannomin
schwannomerlin
neurofibromin-2
moesin-ezrin-radixin like
moesin-ezrin-radixin-like protein
moesin-ezrin-radizin-like protein
neurofibromin 2 (bilateral acoustic neuroma)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

22

22q12.2

Summary This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

607379

Disorder Html: Neurofibromatosis, type 2, 101000 (3); Meningioma, NF2-related,

mRNA and Protein(s)

mRNA Protein Name
NM_000268 NP_000259 merlin isoform 1
NM_016418 NP_057502 merlin isoform 2
NM_181828 NP_861966 merlin isoform 5
NM_181829 NP_861967 merlin isoform 6
NM_181830 NP_861968 merlin isoform 7
NM_181832 NP_861970 merlin isoform 2
NM_181833 NP_861971 merlin isoform 8
NM_181825 NP_861546 merlin isoform 2
NM_181831 NP_861969 merlin isoform 7



Homo sapiens (human) NF2 NP_000259.1
Macaca mulatta (Rhesus monkey) NF2 XP_001106489.1
Canis lupus familiaris (dog) NF2 XP_534729.2
Bos taurus (cattle) NF2 XP_003587221.1
Mus musculus (house mouse) Nf2 NP_035028.2
Rattus norvegicus (Norway rat) Nf2 NP_037325.1
Gallus gallus (chicken) NF2 NP_989828.2
Danio rerio (zebrafish) nf2a NP_001122179.1
Drosophila melanogaster (fruit fly) Mer NP_523413.1
Xenopus (Silurana) tropicalis (western clawed frog) nf2 NP_001072362.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18888
NM_000268 Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
OHu16401 NM_016418 Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu16463 NM_181828 Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu16365 NM_181829 Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu16489
NM_181830 Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
OHu16401 NM_181832 Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu16399 NM_181833 Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu16401 NM_181825 Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu16489
NM_181831 Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18888
Clone ID Related Accession (Same CDS sequence) NM_000268
Accession Version NM_000268.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1788bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product merlin isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN419318.1, AF369657.1, AC004882.3 and BC071653.1. This sequence is a reference standard in the RefSeqGene project. On Dec 20, 2007 this sequence version replaced gi:32967273. Transcript Variant: This variant (1) encodes the longer predominant isoform (1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF369657.1, BC020257.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGATGAAG
TGGAAAGGGA AGGACCTCTT TGATTTGGTG TGCCGGACTC TGGGGCTCCG AGAAACCTGG
TTCTTTGGAC TGCAGTACAC AATCAAGGAC ACAGTGGCCT GGCTCAAAAT GGACAAGAAG
GTACTGGATC ATGATGTTTC AAAGGAAGAA CCAGTCACCT TTCACTTCTT GGCCAAATTT
TATCCTGAGA ATGCTGAAGA GGAGCTGGTT CAGGAGATCA CACAACATTT ATTCTTCTTA
CAGGTAAAGA AGCAGATTTT AGATGAAAAG ATCTACTGCC CTCCTGAGGC TTCTGTGCTC
CTGGCTTCTT ACGCCGTCCA GGCCAAGTAT GGTGACTACG ACCCCAGTGT TCACAAGCGG
GGATTTTTGG CCCAAGAGGA ATTGCTTCCA AAAAGGGTAA TAAATCTGTA TCAGATGACT
CCGGAAATGT GGGAGGAGAG AATTACTGCT TGGTACGCAG AGCACCGAGG CCGAGCCAGG
GATGAAGCTG AAATGGAATA TCTGAAGATA GCTCAGGACC TGGAGATGTA CGGTGTGAAC
TACTTTGCAA TCCGGAATAA AAAGGGCACA GAGCTGCTGC TTGGAGTGGA TGCCCTGGGG
CTTCACATTT ATGACCCTGA GAACAGACTG ACCCCCAAGA TCTCCTTCCC GTGGAATGAA
ATCCGAAACA TCTCGTACAG TGACAAGGAG TTTACTATTA AACCACTGGA TAAGAAAATT
GATGTCTTCA AGTTTAACTC CTCAAAGCTT CGTGTTAATA AGCTGATTCT CCAGCTATGT
ATCGGGAACC ATGATCTATT TATGAGGAGA AGGAAAGCCG ATTCTTTGGA AGTTCAGCAG
ATGAAAGCCC AGGCCAGGGA GGAGAAGGCT AGAAAGCAGA TGGAGCGGCA GCGCCTCGCT
CGAGAGAAGC AGATGAGGGA GGAGGCTGAA CGCACGAGGG ATGAGTTGGA GAGGAGGCTG
CTGCAGATGA AAGAAGAAGC AACAATGGCC AACGAAGCAC TGATGCGGTC TGAGGAGACA
GCTGACCTGT TGGCTGAAAA GGCCCAGATC ACCGAGGAGG AGGCAAAACT TCTGGCCCAG
AAGGCCGCAG AGGCTGAGCA GGAAATGCAG CGCATCAAGG CCACAGCGAT TCGCACGGAG
GAGGAGAAGC GCCTGATGGA GCAGAAGGTG CTGGAAGCCG AGGTGCTGGC ACTGAAGATG
GCTGAGGAGT CAGAGAGGAG GGCCAAAGAG GCAGATCAGC TGAAGCAGGA CCTGCAGGAA
GCACGCGAGG CGGAGCGAAG AGCCAAGCAG AAGCTCCTGG AGATTGCCAC CAAGCCCACG
TACCCGCCCA TGAACCCAAT TCCAGCACCG TTGCCTCCTG ACATACCAAG CTTCAACCTC
ATTGGTGACA GCCTGTCTTT CGACTTCAAA GATACTGACA TGAAGCGGCT TTCCATGGAG
ATAGAGAAAG AAAAAGTGGA ATACATGGAA AAGAGCAAGC ATCTGCAGGA GCAGCTCAAT
GAACTCAAGA CAGAAATCGA GGCCTTGAAA CTGAAAGAGA GGGAGACAGC TCTGGATATT
CTGCACAATG AGAACTCCGA CAGGGGTGGC AGCAGCAAGC ACAATACCAT TAAAAAGCTC
ACCTTGCAGA GCGCCAAGTC CCGAGTGGCC TTCTTTGAAG AGCTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000259.1
CDS444..2231
Misc Feature(1)357..359(+)
Misc Feature(2)510..1109(+)
Misc Feature(3)519..749(+)
Misc Feature(4)783..1085(+)
Misc Feature(5)1089..1379(+)
Misc Feature(6)1215..1355(+)
Misc Feature(7)1215..1355(+)
Misc Feature(8)1323..1337(+)
Misc Feature(9)1332..1379(+)
Misc Feature(10)1404..2066(+)
Misc Feature(11)1482..2228(+)
Misc Feature(12)1995..1997(+)
Misc Feature(13)1995..1997(+)
Misc Feature(14)1995..1997(+)
Exon (1)1..557
Gene:NF2
Gene Synonym:
Exon (2)558..683
Gene:NF2
Gene Synonym:
Exon (3)684..806
Gene:NF2
Gene Synonym:
Exon (4)807..890
Gene:NF2
Gene Synonym:
Exon (5)891..959
Gene:NF2
Gene Synonym:
Exon (6)960..1042
Gene:NF2
Gene Synonym:
Exon (7)1043..1118
Gene:NF2
Gene Synonym:
Exon (8)1119..1253
Gene:NF2
Gene Synonym:
Exon (9)1254..1328
Gene:NF2
Gene Synonym:
Exon (10)1329..1442
Gene:NF2
Gene Synonym:
Exon (11)1443..1565
Gene:NF2
Gene Synonym:
Exon (12)1566..1783
Gene:NF2
Gene Synonym:
Exon (13)1784..1889
Gene:NF2
Gene Synonym:
Exon (14)1890..2017
Gene:NF2
Gene Synonym:
Exon (15)2018..2180
Gene:NF2
Gene Synonym:
Exon (16)2181..6029
Gene:NF2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000268
Organism Homo sapiens (human)
Definition Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000268

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGATGAAG
TGGAAAGGGA AGGACCTCTT TGATTTGGTG TGCCGGACTC TGGGGCTCCG AGAAACCTGG
TTCTTTGGAC TGCAGTACAC AATCAAGGAC ACAGTGGCCT GGCTCAAAAT GGACAAGAAG
GTACTGGATC ATGATGTTTC AAAGGAAGAA CCAGTCACCT TTCACTTCTT GGCCAAATTT
TATCCTGAGA ATGCTGAAGA GGAGCTGGTT CAGGAGATCA CACAACATTT ATTCTTCTTA
CAGGTAAAGA AGCAGATTTT AGATGAAAAG ATCTACTGCC CTCCTGAGGC TTCTGTGCTC
CTGGCTTCTT ACGCCGTCCA GGCCAAGTAT GGTGACTACG ACCCCAGTGT TCACAAGCGG
GGATTTTTGG CCCAAGAGGA ATTGCTTCCA AAAAGGGTAA TAAATCTGTA TCAGATGACT
CCGGAAATGT GGGAGGAGAG AATTACTGCT TGGTACGCAG AGCACCGAGG CCGAGCCAGG
GATGAAGCTG AAATGGAATA TCTGAAGATA GCTCAGGACC TGGAGATGTA CGGTGTGAAC
TACTTTGCAA TCCGGAATAA AAAGGGCACA GAGCTGCTGC TTGGAGTGGA TGCCCTGGGG
CTTCACATTT ATGACCCTGA GAACAGACTG ACCCCCAAGA TCTCCTTCCC GTGGAATGAA
ATCCGAAACA TCTCGTACAG TGACAAGGAG TTTACTATTA AACCACTGGA TAAGAAAATT
GATGTCTTCA AGTTTAACTC CTCAAAGCTT CGTGTTAATA AGCTGATTCT CCAGCTATGT
ATCGGGAACC ATGATCTATT TATGAGGAGA AGGAAAGCCG ATTCTTTGGA AGTTCAGCAG
ATGAAAGCCC AGGCCAGGGA GGAGAAGGCT AGAAAGCAGA TGGAGCGGCA GCGCCTCGCT
CGAGAGAAGC AGATGAGGGA GGAGGCTGAA CGCACGAGGG ATGAGTTGGA GAGGAGGCTG
CTGCAGATGA AAGAAGAAGC AACAATGGCC AACGAAGCAC TGATGCGGTC TGAGGAGACA
GCTGACCTGT TGGCTGAAAA GGCCCAGATC ACCGAGGAGG AGGCAAAACT TCTGGCCCAG
AAGGCCGCAG AGGCTGAGCA GGAAATGCAG CGCATCAAGG CCACAGCGAT TCGCACGGAG
GAGGAGAAGC GCCTGATGGA GCAGAAGGTG CTGGAAGCCG AGGTGCTGGC ACTGAAGATG
GCTGAGGAGT CAGAGAGGAG GGCCAAAGAG GCAGATCAGC TGAAGCAGGA CCTGCAGGAA
GCACGCGAGG CGGAGCGAAG AGCCAAGCAG AAGCTCCTGG AGATTGCCAC CAAGCCCACG
TACCCGCCCA TGAACCCAAT TCCAGCACCG TTGCCTCCTG ACATACCAAG CTTCAACCTC
ATTGGTGACA GCCTGTCTTT CGACTTCAAA GATACTGACA TGAAGCGGCT TTCCATGGAG
ATAGAGAAAG AAAAAGTGGA ATACATGGAA AAGAGCAAGC ATCTGCAGGA GCAGCTCAAT
GAACTCAAGA CAGAAATCGA GGCCTTGAAA CTGAAAGAGA GGGAGACAGC TCTGGATATT
CTGCACAATG AGAACTCCGA CAGGGGTGGC AGCAGCAAGC ACAATACCAT TAAAAAGCTC
ACCTTGCAGA GCGCCAAGTC CCGAGTGGCC TTCTTTGAAG AGCTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16401
Clone ID Related Accession (Same CDS sequence) NM_016418 , NM_181825 , NM_181832
Accession Version NM_016418.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1773bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product merlin isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN419318.1, AF369658.1, AC004882.3 and BC071653.1. This sequence is a reference standard in the RefSeqGene project. On Dec 20, 2007 this sequence version replaced gi:32967274. Transcript Variant: This variant (2) includes an alternate exon, compared to variant 1, that causes a frameshift. The resulting protein (isoform 2), one of the two predominant isoforms, has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF369658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGATGAAG
TGGAAAGGGA AGGACCTCTT TGATTTGGTG TGCCGGACTC TGGGGCTCCG AGAAACCTGG
TTCTTTGGAC TGCAGTACAC AATCAAGGAC ACAGTGGCCT GGCTCAAAAT GGACAAGAAG
GTACTGGATC ATGATGTTTC AAAGGAAGAA CCAGTCACCT TTCACTTCTT GGCCAAATTT
TATCCTGAGA ATGCTGAAGA GGAGCTGGTT CAGGAGATCA CACAACATTT ATTCTTCTTA
CAGGTAAAGA AGCAGATTTT AGATGAAAAG ATCTACTGCC CTCCTGAGGC TTCTGTGCTC
CTGGCTTCTT ACGCCGTCCA GGCCAAGTAT GGTGACTACG ACCCCAGTGT TCACAAGCGG
GGATTTTTGG CCCAAGAGGA ATTGCTTCCA AAAAGGGTAA TAAATCTGTA TCAGATGACT
CCGGAAATGT GGGAGGAGAG AATTACTGCT TGGTACGCAG AGCACCGAGG CCGAGCCAGG
GATGAAGCTG AAATGGAATA TCTGAAGATA GCTCAGGACC TGGAGATGTA CGGTGTGAAC
TACTTTGCAA TCCGGAATAA AAAGGGCACA GAGCTGCTGC TTGGAGTGGA TGCCCTGGGG
CTTCACATTT ATGACCCTGA GAACAGACTG ACCCCCAAGA TCTCCTTCCC GTGGAATGAA
ATCCGAAACA TCTCGTACAG TGACAAGGAG TTTACTATTA AACCACTGGA TAAGAAAATT
GATGTCTTCA AGTTTAACTC CTCAAAGCTT CGTGTTAATA AGCTGATTCT CCAGCTATGT
ATCGGGAACC ATGATCTATT TATGAGGAGA AGGAAAGCCG ATTCTTTGGA AGTTCAGCAG
ATGAAAGCCC AGGCCAGGGA GGAGAAGGCT AGAAAGCAGA TGGAGCGGCA GCGCCTCGCT
CGAGAGAAGC AGATGAGGGA GGAGGCTGAA CGCACGAGGG ATGAGTTGGA GAGGAGGCTG
CTGCAGATGA AAGAAGAAGC AACAATGGCC AACGAAGCAC TGATGCGGTC TGAGGAGACA
GCTGACCTGT TGGCTGAAAA GGCCCAGATC ACCGAGGAGG AGGCAAAACT TCTGGCCCAG
AAGGCCGCAG AGGCTGAGCA GGAAATGCAG CGCATCAAGG CCACAGCGAT TCGCACGGAG
GAGGAGAAGC GCCTGATGGA GCAGAAGGTG CTGGAAGCCG AGGTGCTGGC ACTGAAGATG
GCTGAGGAGT CAGAGAGGAG GGCCAAAGAG GCAGATCAGC TGAAGCAGGA CCTGCAGGAA
GCACGCGAGG CGGAGCGAAG AGCCAAGCAG AAGCTCCTGG AGATTGCCAC CAAGCCCACG
TACCCGCCCA TGAACCCAAT TCCAGCACCG TTGCCTCCTG ACATACCAAG CTTCAACCTC
ATTGGTGACA GCCTGTCTTT CGACTTCAAA GATACTGACA TGAAGCGGCT TTCCATGGAG
ATAGAGAAAG AAAAAGTGGA ATACATGGAA AAGAGCAAGC ATCTGCAGGA GCAGCTCAAT
GAACTCAAGA CAGAAATCGA GGCCTTGAAA CTGAAAGAGA GGGAGACAGC TCTGGATATT
CTGCACAATG AGAACTCCGA CAGGGGTGGC AGCAGCAAGC ACAATACCAT TAAAAAGCCT
CAAGCCCAAG GCAGAAGACC TATCTGCATT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_057502.2
CDS444..2216
Misc Feature(1)357..359(+)
Misc Feature(2)510..1109(+)
Misc Feature(3)519..749(+)
Misc Feature(4)783..1085(+)
Misc Feature(5)1089..1379(+)
Misc Feature(6)1215..1355(+)
Misc Feature(7)1215..1355(+)
Misc Feature(8)1323..1337(+)
Misc Feature(9)1332..1379(+)
Misc Feature(10)1404..2066(+)
Misc Feature(11)1482..2180(+)
Misc Feature(12)1995..1997(+)
Misc Feature(13)1995..1997(+)
Misc Feature(14)1995..1997(+)
Exon (1)1..557
Gene:NF2
Gene Synonym:
Exon (2)558..683
Gene:NF2
Gene Synonym:
Exon (3)684..806
Gene:NF2
Gene Synonym:
Exon (4)807..890
Gene:NF2
Gene Synonym:
Exon (5)891..959
Gene:NF2
Gene Synonym:
Exon (6)960..1042
Gene:NF2
Gene Synonym:
Exon (7)1043..1118
Gene:NF2
Gene Synonym:
Exon (8)1119..1253
Gene:NF2
Gene Synonym:
Exon (9)1254..1328
Gene:NF2
Gene Synonym:
Exon (10)1329..1442
Gene:NF2
Gene Synonym:
Exon (11)1443..1565
Gene:NF2
Gene Synonym:
Exon (12)1566..1783
Gene:NF2
Gene Synonym:
Exon (13)1784..1889
Gene:NF2
Gene Synonym:
Exon (14)1890..2017
Gene:NF2
Gene Synonym:
Exon (15)2018..2180
Gene:NF2
Gene Synonym:
Exon (16)2181..2225
Gene:NF2
Gene Synonym:
Exon (17)2226..6074
Gene:NF2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_016418
Organism Homo sapiens (human)
Definition Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_016418

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGATGAAG
TGGAAAGGGA AGGACCTCTT TGATTTGGTG TGCCGGACTC TGGGGCTCCG AGAAACCTGG
TTCTTTGGAC TGCAGTACAC AATCAAGGAC ACAGTGGCCT GGCTCAAAAT GGACAAGAAG
GTACTGGATC ATGATGTTTC AAAGGAAGAA CCAGTCACCT TTCACTTCTT GGCCAAATTT
TATCCTGAGA ATGCTGAAGA GGAGCTGGTT CAGGAGATCA CACAACATTT ATTCTTCTTA
CAGGTAAAGA AGCAGATTTT AGATGAAAAG ATCTACTGCC CTCCTGAGGC TTCTGTGCTC
CTGGCTTCTT ACGCCGTCCA GGCCAAGTAT GGTGACTACG ACCCCAGTGT TCACAAGCGG
GGATTTTTGG CCCAAGAGGA ATTGCTTCCA AAAAGGGTAA TAAATCTGTA TCAGATGACT
CCGGAAATGT GGGAGGAGAG AATTACTGCT TGGTACGCAG AGCACCGAGG CCGAGCCAGG
GATGAAGCTG AAATGGAATA TCTGAAGATA GCTCAGGACC TGGAGATGTA CGGTGTGAAC
TACTTTGCAA TCCGGAATAA AAAGGGCACA GAGCTGCTGC TTGGAGTGGA TGCCCTGGGG
CTTCACATTT ATGACCCTGA GAACAGACTG ACCCCCAAGA TCTCCTTCCC GTGGAATGAA
ATCCGAAACA TCTCGTACAG TGACAAGGAG TTTACTATTA AACCACTGGA TAAGAAAATT
GATGTCTTCA AGTTTAACTC CTCAAAGCTT CGTGTTAATA AGCTGATTCT CCAGCTATGT
ATCGGGAACC ATGATCTATT TATGAGGAGA AGGAAAGCCG ATTCTTTGGA AGTTCAGCAG
ATGAAAGCCC AGGCCAGGGA GGAGAAGGCT AGAAAGCAGA TGGAGCGGCA GCGCCTCGCT
CGAGAGAAGC AGATGAGGGA GGAGGCTGAA CGCACGAGGG ATGAGTTGGA GAGGAGGCTG
CTGCAGATGA AAGAAGAAGC AACAATGGCC AACGAAGCAC TGATGCGGTC TGAGGAGACA
GCTGACCTGT TGGCTGAAAA GGCCCAGATC ACCGAGGAGG AGGCAAAACT TCTGGCCCAG
AAGGCCGCAG AGGCTGAGCA GGAAATGCAG CGCATCAAGG CCACAGCGAT TCGCACGGAG
GAGGAGAAGC GCCTGATGGA GCAGAAGGTG CTGGAAGCCG AGGTGCTGGC ACTGAAGATG
GCTGAGGAGT CAGAGAGGAG GGCCAAAGAG GCAGATCAGC TGAAGCAGGA CCTGCAGGAA
GCACGCGAGG CGGAGCGAAG AGCCAAGCAG AAGCTCCTGG AGATTGCCAC CAAGCCCACG
TACCCGCCCA TGAACCCAAT TCCAGCACCG TTGCCTCCTG ACATACCAAG CTTCAACCTC
ATTGGTGACA GCCTGTCTTT CGACTTCAAA GATACTGACA TGAAGCGGCT TTCCATGGAG
ATAGAGAAAG AAAAAGTGGA ATACATGGAA AAGAGCAAGC ATCTGCAGGA GCAGCTCAAT
GAACTCAAGA CAGAAATCGA GGCCTTGAAA CTGAAAGAGA GGGAGACAGC TCTGGATATT
CTGCACAATG AGAACTCCGA CAGGGGTGGC AGCAGCAAGC ACAATACCAT TAAAAAGCCT
CAAGCCCAAG GCAGAAGACC TATCTGCATT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16463
Clone ID Related Accession (Same CDS sequence) NM_181828
Accession Version NM_181828.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1647bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product merlin isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN419318.1, AF369658.1, AF369663.1, AC004882.3 and BC071653.1. On Dec 20, 2007 this sequence version replaced gi:32967259. Transcript Variant: This variant (5) lacks an alternate in-frame exon in the 5' coding region and includes an alternate exon in the 3' coding region, compared to variant 1. The resulting protein (isoform 5, also referred to as isoform delE2) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF369663.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGGTACTG
GATCATGATG TTTCAAAGGA AGAACCAGTC ACCTTTCACT TCTTGGCCAA ATTTTATCCT
GAGAATGCTG AAGAGGAGCT GGTTCAGGAG ATCACACAAC ATTTATTCTT CTTACAGGTA
AAGAAGCAGA TTTTAGATGA AAAGATCTAC TGCCCTCCTG AGGCTTCTGT GCTCCTGGCT
TCTTACGCCG TCCAGGCCAA GTATGGTGAC TACGACCCCA GTGTTCACAA GCGGGGATTT
TTGGCCCAAG AGGAATTGCT TCCAAAAAGG GTAATAAATC TGTATCAGAT GACTCCGGAA
ATGTGGGAGG AGAGAATTAC TGCTTGGTAC GCAGAGCACC GAGGCCGAGC CAGGGATGAA
GCTGAAATGG AATATCTGAA GATAGCTCAG GACCTGGAGA TGTACGGTGT GAACTACTTT
GCAATCCGGA ATAAAAAGGG CACAGAGCTG CTGCTTGGAG TGGATGCCCT GGGGCTTCAC
ATTTATGACC CTGAGAACAG ACTGACCCCC AAGATCTCCT TCCCGTGGAA TGAAATCCGA
AACATCTCGT ACAGTGACAA GGAGTTTACT ATTAAACCAC TGGATAAGAA AATTGATGTC
TTCAAGTTTA ACTCCTCAAA GCTTCGTGTT AATAAGCTGA TTCTCCAGCT ATGTATCGGG
AACCATGATC TATTTATGAG GAGAAGGAAA GCCGATTCTT TGGAAGTTCA GCAGATGAAA
GCCCAGGCCA GGGAGGAGAA GGCTAGAAAG CAGATGGAGC GGCAGCGCCT CGCTCGAGAG
AAGCAGATGA GGGAGGAGGC TGAACGCACG AGGGATGAGT TGGAGAGGAG GCTGCTGCAG
ATGAAAGAAG AAGCAACAAT GGCCAACGAA GCACTGATGC GGTCTGAGGA GACAGCTGAC
CTGTTGGCTG AAAAGGCCCA GATCACCGAG GAGGAGGCAA AACTTCTGGC CCAGAAGGCC
GCAGAGGCTG AGCAGGAAAT GCAGCGCATC AAGGCCACAG CGATTCGCAC GGAGGAGGAG
AAGCGCCTGA TGGAGCAGAA GGTGCTGGAA GCCGAGGTGC TGGCACTGAA GATGGCTGAG
GAGTCAGAGA GGAGGGCCAA AGAGGCAGAT CAGCTGAAGC AGGACCTGCA GGAAGCACGC
GAGGCGGAGC GAAGAGCCAA GCAGAAGCTC CTGGAGATTG CCACCAAGCC CACGTACCCG
CCCATGAACC CAATTCCAGC ACCGTTGCCT CCTGACATAC CAAGCTTCAA CCTCATTGGT
GACAGCCTGT CTTTCGACTT CAAAGATACT GACATGAAGC GGCTTTCCAT GGAGATAGAG
AAAGAAAAAG TGGAATACAT GGAAAAGAGC AAGCATCTGC AGGAGCAGCT CAATGAACTC
AAGACAGAAA TCGAGGCCTT GAAACTGAAA GAGAGGGAGA CAGCTCTGGA TATTCTGCAC
AATGAGAACT CCGACAGGGG TGGCAGCAGC AAGCACAATA CCATTAAAAA GCCTCAAGCC
CAAGGCAGAA GACCTATCTG CATTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_861966.1
CDS444..2090
Misc Feature(1)357..359(+)
Misc Feature(2)555..983(+)
Misc Feature(3)657..959(+)
Misc Feature(4)963..1253(+)
Misc Feature(5)1089..1229(+)
Misc Feature(6)1089..1229(+)
Misc Feature(7)1197..1211(+)
Misc Feature(8)1206..1253(+)
Misc Feature(9)1278..1940(+)
Misc Feature(10)1356..2054(+)
Misc Feature(11)1869..1871(+)
Misc Feature(12)1869..1871(+)
Exon (1)1..557
Gene:NF2
Gene Synonym:
Exon (2)558..680
Gene:NF2
Gene Synonym:
Exon (3)681..764
Gene:NF2
Gene Synonym:
Exon (4)765..833
Gene:NF2
Gene Synonym:
Exon (5)834..916
Gene:NF2
Gene Synonym:
Exon (6)917..992
Gene:NF2
Gene Synonym:
Exon (7)993..1127
Gene:NF2
Gene Synonym:
Exon (8)1128..1202
Gene:NF2
Gene Synonym:
Exon (9)1203..1316
Gene:NF2
Gene Synonym:
Exon (10)1317..1439
Gene:NF2
Gene Synonym:
Exon (11)1440..1657
Gene:NF2
Gene Synonym:
Exon (12)1658..1763
Gene:NF2
Gene Synonym:
Exon (13)1764..1891
Gene:NF2
Gene Synonym:
Exon (14)1892..2054
Gene:NF2
Gene Synonym:
Exon (15)2055..2099
Gene:NF2
Gene Synonym:
Exon (16)2100..5948
Gene:NF2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_181828
Organism Homo sapiens (human)
Definition Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_181828

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGGTACTG
GATCATGATG TTTCAAAGGA AGAACCAGTC ACCTTTCACT TCTTGGCCAA ATTTTATCCT
GAGAATGCTG AAGAGGAGCT GGTTCAGGAG ATCACACAAC ATTTATTCTT CTTACAGGTA
AAGAAGCAGA TTTTAGATGA AAAGATCTAC TGCCCTCCTG AGGCTTCTGT GCTCCTGGCT
TCTTACGCCG TCCAGGCCAA GTATGGTGAC TACGACCCCA GTGTTCACAA GCGGGGATTT
TTGGCCCAAG AGGAATTGCT TCCAAAAAGG GTAATAAATC TGTATCAGAT GACTCCGGAA
ATGTGGGAGG AGAGAATTAC TGCTTGGTAC GCAGAGCACC GAGGCCGAGC CAGGGATGAA
GCTGAAATGG AATATCTGAA GATAGCTCAG GACCTGGAGA TGTACGGTGT GAACTACTTT
GCAATCCGGA ATAAAAAGGG CACAGAGCTG CTGCTTGGAG TGGATGCCCT GGGGCTTCAC
ATTTATGACC CTGAGAACAG ACTGACCCCC AAGATCTCCT TCCCGTGGAA TGAAATCCGA
AACATCTCGT ACAGTGACAA GGAGTTTACT ATTAAACCAC TGGATAAGAA AATTGATGTC
TTCAAGTTTA ACTCCTCAAA GCTTCGTGTT AATAAGCTGA TTCTCCAGCT ATGTATCGGG
AACCATGATC TATTTATGAG GAGAAGGAAA GCCGATTCTT TGGAAGTTCA GCAGATGAAA
GCCCAGGCCA GGGAGGAGAA GGCTAGAAAG CAGATGGAGC GGCAGCGCCT CGCTCGAGAG
AAGCAGATGA GGGAGGAGGC TGAACGCACG AGGGATGAGT TGGAGAGGAG GCTGCTGCAG
ATGAAAGAAG AAGCAACAAT GGCCAACGAA GCACTGATGC GGTCTGAGGA GACAGCTGAC
CTGTTGGCTG AAAAGGCCCA GATCACCGAG GAGGAGGCAA AACTTCTGGC CCAGAAGGCC
GCAGAGGCTG AGCAGGAAAT GCAGCGCATC AAGGCCACAG CGATTCGCAC GGAGGAGGAG
AAGCGCCTGA TGGAGCAGAA GGTGCTGGAA GCCGAGGTGC TGGCACTGAA GATGGCTGAG
GAGTCAGAGA GGAGGGCCAA AGAGGCAGAT CAGCTGAAGC AGGACCTGCA GGAAGCACGC
GAGGCGGAGC GAAGAGCCAA GCAGAAGCTC CTGGAGATTG CCACCAAGCC CACGTACCCG
CCCATGAACC CAATTCCAGC ACCGTTGCCT CCTGACATAC CAAGCTTCAA CCTCATTGGT
GACAGCCTGT CTTTCGACTT CAAAGATACT GACATGAAGC GGCTTTCCAT GGAGATAGAG
AAAGAAAAAG TGGAATACAT GGAAAAGAGC AAGCATCTGC AGGAGCAGCT CAATGAACTC
AAGACAGAAA TCGAGGCCTT GAAACTGAAA GAGAGGGAGA CAGCTCTGGA TATTCTGCAC
AATGAGAACT CCGACAGGGG TGGCAGCAGC AAGCACAATA CCATTAAAAA GCCTCAAGCC
CAAGGCAGAA GACCTATCTG CATTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16365
Clone ID Related Accession (Same CDS sequence) NM_181829
Accession Version NM_181829.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1650bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product merlin isoform 6
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN419318.1, CF138178.1, AF369664.1, AC004882.3 and BC071653.1. On Dec 20, 2007 this sequence version replaced gi:32967261. Transcript Variant: This variant (6) lacks an alternate in-frame exon in the 5' coding region and includes an alternate exon in the 3' coding region, compared to variant 1. The resulting protein (isoform 6, also referred to as isoform delE3) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF369664.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGATGAAG
TGGAAAGGGA AGGACCTCTT TGATTTGGTG TGCCGGACTC TGGGGCTCCG AGAAACCTGG
TTCTTTGGAC TGCAGTACAC AATCAAGGAC ACAGTGGCCT GGCTCAAAAT GGACAAGAAG
GTAAAGAAGC AGATTTTAGA TGAAAAGATC TACTGCCCTC CTGAGGCTTC TGTGCTCCTG
GCTTCTTACG CCGTCCAGGC CAAGTATGGT GACTACGACC CCAGTGTTCA CAAGCGGGGA
TTTTTGGCCC AAGAGGAATT GCTTCCAAAA AGGGTAATAA ATCTGTATCA GATGACTCCG
GAAATGTGGG AGGAGAGAAT TACTGCTTGG TACGCAGAGC ACCGAGGCCG AGCCAGGGAT
GAAGCTGAAA TGGAATATCT GAAGATAGCT CAGGACCTGG AGATGTACGG TGTGAACTAC
TTTGCAATCC GGAATAAAAA GGGCACAGAG CTGCTGCTTG GAGTGGATGC CCTGGGGCTT
CACATTTATG ACCCTGAGAA CAGACTGACC CCCAAGATCT CCTTCCCGTG GAATGAAATC
CGAAACATCT CGTACAGTGA CAAGGAGTTT ACTATTAAAC CACTGGATAA GAAAATTGAT
GTCTTCAAGT TTAACTCCTC AAAGCTTCGT GTTAATAAGC TGATTCTCCA GCTATGTATC
GGGAACCATG ATCTATTTAT GAGGAGAAGG AAAGCCGATT CTTTGGAAGT TCAGCAGATG
AAAGCCCAGG CCAGGGAGGA GAAGGCTAGA AAGCAGATGG AGCGGCAGCG CCTCGCTCGA
GAGAAGCAGA TGAGGGAGGA GGCTGAACGC ACGAGGGATG AGTTGGAGAG GAGGCTGCTG
CAGATGAAAG AAGAAGCAAC AATGGCCAAC GAAGCACTGA TGCGGTCTGA GGAGACAGCT
GACCTGTTGG CTGAAAAGGC CCAGATCACC GAGGAGGAGG CAAAACTTCT GGCCCAGAAG
GCCGCAGAGG CTGAGCAGGA AATGCAGCGC ATCAAGGCCA CAGCGATTCG CACGGAGGAG
GAGAAGCGCC TGATGGAGCA GAAGGTGCTG GAAGCCGAGG TGCTGGCACT GAAGATGGCT
GAGGAGTCAG AGAGGAGGGC CAAAGAGGCA GATCAGCTGA AGCAGGACCT GCAGGAAGCA
CGCGAGGCGG AGCGAAGAGC CAAGCAGAAG CTCCTGGAGA TTGCCACCAA GCCCACGTAC
CCGCCCATGA ACCCAATTCC AGCACCGTTG CCTCCTGACA TACCAAGCTT CAACCTCATT
GGTGACAGCC TGTCTTTCGA CTTCAAAGAT ACTGACATGA AGCGGCTTTC CATGGAGATA
GAGAAAGAAA AAGTGGAATA CATGGAAAAG AGCAAGCATC TGCAGGAGCA GCTCAATGAA
CTCAAGACAG AAATCGAGGC CTTGAAACTG AAAGAGAGGG AGACAGCTCT GGATATTCTG
CACAATGAGA ACTCCGACAG GGGTGGCAGC AGCAAGCACA ATACCATTAA AAAGCCTCAA
GCCCAAGGCA GAAGACCTAT CTGCATTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_861967.1
CDS444..2093
Misc Feature(1)357..359(+)
Misc Feature(2)510..986(+)
Misc Feature(3)519..719(+)
Misc Feature(4)684..962(+)
Misc Feature(5)966..1256(+)
Misc Feature(6)1092..1232(+)
Misc Feature(7)1092..1232(+)
Misc Feature(8)1200..1214(+)
Misc Feature(9)1209..1256(+)
Misc Feature(10)1281..1943(+)
Misc Feature(11)1359..2057(+)
Misc Feature(12)1872..1874(+)
Misc Feature(13)1872..1874(+)
Exon (1)1..557
Gene:NF2
Gene Synonym:
Exon (2)558..683
Gene:NF2
Gene Synonym:
Exon (3)684..767
Gene:NF2
Gene Synonym:
Exon (4)768..836
Gene:NF2
Gene Synonym:
Exon (5)837..919
Gene:NF2
Gene Synonym:
Exon (6)920..995
Gene:NF2
Gene Synonym:
Exon (7)996..1130
Gene:NF2
Gene Synonym:
Exon (8)1131..1205
Gene:NF2
Gene Synonym:
Exon (9)1206..1319
Gene:NF2
Gene Synonym:
Exon (10)1320..1442
Gene:NF2
Gene Synonym:
Exon (11)1443..1660
Gene:NF2
Gene Synonym:
Exon (12)1661..1766
Gene:NF2
Gene Synonym:
Exon (13)1767..1894
Gene:NF2
Gene Synonym:
Exon (14)1895..2057
Gene:NF2
Gene Synonym:
Exon (15)2058..2102
Gene:NF2
Gene Synonym:
Exon (16)2103..5951
Gene:NF2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_181829
Organism Homo sapiens (human)
Definition Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_181829

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGATGAAG
TGGAAAGGGA AGGACCTCTT TGATTTGGTG TGCCGGACTC TGGGGCTCCG AGAAACCTGG
TTCTTTGGAC TGCAGTACAC AATCAAGGAC ACAGTGGCCT GGCTCAAAAT GGACAAGAAG
GTAAAGAAGC AGATTTTAGA TGAAAAGATC TACTGCCCTC CTGAGGCTTC TGTGCTCCTG
GCTTCTTACG CCGTCCAGGC CAAGTATGGT GACTACGACC CCAGTGTTCA CAAGCGGGGA
TTTTTGGCCC AAGAGGAATT GCTTCCAAAA AGGGTAATAA ATCTGTATCA GATGACTCCG
GAAATGTGGG AGGAGAGAAT TACTGCTTGG TACGCAGAGC ACCGAGGCCG AGCCAGGGAT
GAAGCTGAAA TGGAATATCT GAAGATAGCT CAGGACCTGG AGATGTACGG TGTGAACTAC
TTTGCAATCC GGAATAAAAA GGGCACAGAG CTGCTGCTTG GAGTGGATGC CCTGGGGCTT
CACATTTATG ACCCTGAGAA CAGACTGACC CCCAAGATCT CCTTCCCGTG GAATGAAATC
CGAAACATCT CGTACAGTGA CAAGGAGTTT ACTATTAAAC CACTGGATAA GAAAATTGAT
GTCTTCAAGT TTAACTCCTC AAAGCTTCGT GTTAATAAGC TGATTCTCCA GCTATGTATC
GGGAACCATG ATCTATTTAT GAGGAGAAGG AAAGCCGATT CTTTGGAAGT TCAGCAGATG
AAAGCCCAGG CCAGGGAGGA GAAGGCTAGA AAGCAGATGG AGCGGCAGCG CCTCGCTCGA
GAGAAGCAGA TGAGGGAGGA GGCTGAACGC ACGAGGGATG AGTTGGAGAG GAGGCTGCTG
CAGATGAAAG AAGAAGCAAC AATGGCCAAC GAAGCACTGA TGCGGTCTGA GGAGACAGCT
GACCTGTTGG CTGAAAAGGC CCAGATCACC GAGGAGGAGG CAAAACTTCT GGCCCAGAAG
GCCGCAGAGG CTGAGCAGGA AATGCAGCGC ATCAAGGCCA CAGCGATTCG CACGGAGGAG
GAGAAGCGCC TGATGGAGCA GAAGGTGCTG GAAGCCGAGG TGCTGGCACT GAAGATGGCT
GAGGAGTCAG AGAGGAGGGC CAAAGAGGCA GATCAGCTGA AGCAGGACCT GCAGGAAGCA
CGCGAGGCGG AGCGAAGAGC CAAGCAGAAG CTCCTGGAGA TTGCCACCAA GCCCACGTAC
CCGCCCATGA ACCCAATTCC AGCACCGTTG CCTCCTGACA TACCAAGCTT CAACCTCATT
GGTGACAGCC TGTCTTTCGA CTTCAAAGAT ACTGACATGA AGCGGCTTTC CATGGAGATA
GAGAAAGAAA AAGTGGAATA CATGGAAAAG AGCAAGCATC TGCAGGAGCA GCTCAATGAA
CTCAAGACAG AAATCGAGGC CTTGAAACTG AAAGAGAGGG AGACAGCTCT GGATATTCTG
CACAATGAGA ACTCCGACAG GGGTGGCAGC AGCAAGCACA ATACCATTAA AAAGCCTCAA
GCCCAAGGCA GAAGACCTAT CTGCATTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16489
Clone ID Related Accession (Same CDS sequence) NM_181830 , NM_181831
Accession Version NM_181830.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1524bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product merlin isoform 7
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN419318.1, AF369657.1, AF369665.1, AC004882.3 and BC071653.1. On Dec 20, 2007 this sequence version replaced gi:32967263. Transcript Variant: This variant (7) lacks two alternate in-frame exons in the 5' coding region and includes an alternate exon in the 3' coding region, compared to variant 1. The resulting protein (isoform 7, also referred to as isoform delE2/3) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF369665.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGGTAAAG
AAGCAGATTT TAGATGAAAA GATCTACTGC CCTCCTGAGG CTTCTGTGCT CCTGGCTTCT
TACGCCGTCC AGGCCAAGTA TGGTGACTAC GACCCCAGTG TTCACAAGCG GGGATTTTTG
GCCCAAGAGG AATTGCTTCC AAAAAGGGTA ATAAATCTGT ATCAGATGAC TCCGGAAATG
TGGGAGGAGA GAATTACTGC TTGGTACGCA GAGCACCGAG GCCGAGCCAG GGATGAAGCT
GAAATGGAAT ATCTGAAGAT AGCTCAGGAC CTGGAGATGT ACGGTGTGAA CTACTTTGCA
ATCCGGAATA AAAAGGGCAC AGAGCTGCTG CTTGGAGTGG ATGCCCTGGG GCTTCACATT
TATGACCCTG AGAACAGACT GACCCCCAAG ATCTCCTTCC CGTGGAATGA AATCCGAAAC
ATCTCGTACA GTGACAAGGA GTTTACTATT AAACCACTGG ATAAGAAAAT TGATGTCTTC
AAGTTTAACT CCTCAAAGCT TCGTGTTAAT AAGCTGATTC TCCAGCTATG TATCGGGAAC
CATGATCTAT TTATGAGGAG AAGGAAAGCC GATTCTTTGG AAGTTCAGCA GATGAAAGCC
CAGGCCAGGG AGGAGAAGGC TAGAAAGCAG ATGGAGCGGC AGCGCCTCGC TCGAGAGAAG
CAGATGAGGG AGGAGGCTGA ACGCACGAGG GATGAGTTGG AGAGGAGGCT GCTGCAGATG
AAAGAAGAAG CAACAATGGC CAACGAAGCA CTGATGCGGT CTGAGGAGAC AGCTGACCTG
TTGGCTGAAA AGGCCCAGAT CACCGAGGAG GAGGCAAAAC TTCTGGCCCA GAAGGCCGCA
GAGGCTGAGC AGGAAATGCA GCGCATCAAG GCCACAGCGA TTCGCACGGA GGAGGAGAAG
CGCCTGATGG AGCAGAAGGT GCTGGAAGCC GAGGTGCTGG CACTGAAGAT GGCTGAGGAG
TCAGAGAGGA GGGCCAAAGA GGCAGATCAG CTGAAGCAGG ACCTGCAGGA AGCACGCGAG
GCGGAGCGAA GAGCCAAGCA GAAGCTCCTG GAGATTGCCA CCAAGCCCAC GTACCCGCCC
ATGAACCCAA TTCCAGCACC GTTGCCTCCT GACATACCAA GCTTCAACCT CATTGGTGAC
AGCCTGTCTT TCGACTTCAA AGATACTGAC ATGAAGCGGC TTTCCATGGA GATAGAGAAA
GAAAAAGTGG AATACATGGA AAAGAGCAAG CATCTGCAGG AGCAGCTCAA TGAACTCAAG
ACAGAAATCG AGGCCTTGAA ACTGAAAGAG AGGGAGACAG CTCTGGATAT TCTGCACAAT
GAGAACTCCG ACAGGGGTGG CAGCAGCAAG CACAATACCA TTAAAAAGCC TCAAGCCCAA
GGCAGAAGAC CTATCTGCAT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_861968.1
CDS444..1967
Misc Feature(1)357..359(+)
Misc Feature(2)552..860(+)
Misc Feature(3)558..836(+)
Misc Feature(4)840..1130(+)
Misc Feature(5)966..1106(+)
Misc Feature(6)966..1106(+)
Misc Feature(7)1074..1088(+)
Misc Feature(8)1083..1130(+)
Misc Feature(9)1155..1817(+)
Misc Feature(10)1233..1931(+)
Misc Feature(11)1746..1748(+)
Misc Feature(12)1746..1748(+)
Exon (1)1..557
Gene:NF2
Gene Synonym:
Exon (2)558..641
Gene:NF2
Gene Synonym:
Exon (3)642..710
Gene:NF2
Gene Synonym:
Exon (4)711..793
Gene:NF2
Gene Synonym:
Exon (5)794..869
Gene:NF2
Gene Synonym:
Exon (6)870..1004
Gene:NF2
Gene Synonym:
Exon (7)1005..1079
Gene:NF2
Gene Synonym:
Exon (8)1080..1193
Gene:NF2
Gene Synonym:
Exon (9)1194..1316
Gene:NF2
Gene Synonym:
Exon (10)1317..1534
Gene:NF2
Gene Synonym:
Exon (11)1535..1640
Gene:NF2
Gene Synonym:
Exon (12)1641..1768
Gene:NF2
Gene Synonym:
Exon (13)1769..1931
Gene:NF2
Gene Synonym:
Exon (14)1932..1976
Gene:NF2
Gene Synonym:
Exon (15)1977..5825
Gene:NF2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_181830
Organism Homo sapiens (human)
Definition Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_181830

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGGTAAAG
AAGCAGATTT TAGATGAAAA GATCTACTGC CCTCCTGAGG CTTCTGTGCT CCTGGCTTCT
TACGCCGTCC AGGCCAAGTA TGGTGACTAC GACCCCAGTG TTCACAAGCG GGGATTTTTG
GCCCAAGAGG AATTGCTTCC AAAAAGGGTA ATAAATCTGT ATCAGATGAC TCCGGAAATG
TGGGAGGAGA GAATTACTGC TTGGTACGCA GAGCACCGAG GCCGAGCCAG GGATGAAGCT
GAAATGGAAT ATCTGAAGAT AGCTCAGGAC CTGGAGATGT ACGGTGTGAA CTACTTTGCA
ATCCGGAATA AAAAGGGCAC AGAGCTGCTG CTTGGAGTGG ATGCCCTGGG GCTTCACATT
TATGACCCTG AGAACAGACT GACCCCCAAG ATCTCCTTCC CGTGGAATGA AATCCGAAAC
ATCTCGTACA GTGACAAGGA GTTTACTATT AAACCACTGG ATAAGAAAAT TGATGTCTTC
AAGTTTAACT CCTCAAAGCT TCGTGTTAAT AAGCTGATTC TCCAGCTATG TATCGGGAAC
CATGATCTAT TTATGAGGAG AAGGAAAGCC GATTCTTTGG AAGTTCAGCA GATGAAAGCC
CAGGCCAGGG AGGAGAAGGC TAGAAAGCAG ATGGAGCGGC AGCGCCTCGC TCGAGAGAAG
CAGATGAGGG AGGAGGCTGA ACGCACGAGG GATGAGTTGG AGAGGAGGCT GCTGCAGATG
AAAGAAGAAG CAACAATGGC CAACGAAGCA CTGATGCGGT CTGAGGAGAC AGCTGACCTG
TTGGCTGAAA AGGCCCAGAT CACCGAGGAG GAGGCAAAAC TTCTGGCCCA GAAGGCCGCA
GAGGCTGAGC AGGAAATGCA GCGCATCAAG GCCACAGCGA TTCGCACGGA GGAGGAGAAG
CGCCTGATGG AGCAGAAGGT GCTGGAAGCC GAGGTGCTGG CACTGAAGAT GGCTGAGGAG
TCAGAGAGGA GGGCCAAAGA GGCAGATCAG CTGAAGCAGG ACCTGCAGGA AGCACGCGAG
GCGGAGCGAA GAGCCAAGCA GAAGCTCCTG GAGATTGCCA CCAAGCCCAC GTACCCGCCC
ATGAACCCAA TTCCAGCACC GTTGCCTCCT GACATACCAA GCTTCAACCT CATTGGTGAC
AGCCTGTCTT TCGACTTCAA AGATACTGAC ATGAAGCGGC TTTCCATGGA GATAGAGAAA
GAAAAAGTGG AATACATGGA AAAGAGCAAG CATCTGCAGG AGCAGCTCAA TGAACTCAAG
ACAGAAATCG AGGCCTTGAA ACTGAAAGAG AGGGAGACAG CTCTGGATAT TCTGCACAAT
GAGAACTCCG ACAGGGGTGG CAGCAGCAAG CACAATACCA TTAAAAAGCC TCAAGCCCAA
GGCAGAAGAC CTATCTGCAT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16401
Clone ID Related Accession (Same CDS sequence) NM_016418 , NM_181825 , NM_181832
Accession Version NM_181832.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1773bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product merlin isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN419318.1, CF138178.1, AF369668.1, AC004882.3 and BC071653.1. On Dec 20, 2007 this sequence version replaced gi:32967265. Transcript Variant: This variant (8) includes an alternate exon, compared to variant 1, that causes a frameshift. The resulting protein (isoform 2), one of the two predominant isoforms, has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF369668.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGATGAAG
TGGAAAGGGA AGGACCTCTT TGATTTGGTG TGCCGGACTC TGGGGCTCCG AGAAACCTGG
TTCTTTGGAC TGCAGTACAC AATCAAGGAC ACAGTGGCCT GGCTCAAAAT GGACAAGAAG
GTACTGGATC ATGATGTTTC AAAGGAAGAA CCAGTCACCT TTCACTTCTT GGCCAAATTT
TATCCTGAGA ATGCTGAAGA GGAGCTGGTT CAGGAGATCA CACAACATTT ATTCTTCTTA
CAGGTAAAGA AGCAGATTTT AGATGAAAAG ATCTACTGCC CTCCTGAGGC TTCTGTGCTC
CTGGCTTCTT ACGCCGTCCA GGCCAAGTAT GGTGACTACG ACCCCAGTGT TCACAAGCGG
GGATTTTTGG CCCAAGAGGA ATTGCTTCCA AAAAGGGTAA TAAATCTGTA TCAGATGACT
CCGGAAATGT GGGAGGAGAG AATTACTGCT TGGTACGCAG AGCACCGAGG CCGAGCCAGG
GATGAAGCTG AAATGGAATA TCTGAAGATA GCTCAGGACC TGGAGATGTA CGGTGTGAAC
TACTTTGCAA TCCGGAATAA AAAGGGCACA GAGCTGCTGC TTGGAGTGGA TGCCCTGGGG
CTTCACATTT ATGACCCTGA GAACAGACTG ACCCCCAAGA TCTCCTTCCC GTGGAATGAA
ATCCGAAACA TCTCGTACAG TGACAAGGAG TTTACTATTA AACCACTGGA TAAGAAAATT
GATGTCTTCA AGTTTAACTC CTCAAAGCTT CGTGTTAATA AGCTGATTCT CCAGCTATGT
ATCGGGAACC ATGATCTATT TATGAGGAGA AGGAAAGCCG ATTCTTTGGA AGTTCAGCAG
ATGAAAGCCC AGGCCAGGGA GGAGAAGGCT AGAAAGCAGA TGGAGCGGCA GCGCCTCGCT
CGAGAGAAGC AGATGAGGGA GGAGGCTGAA CGCACGAGGG ATGAGTTGGA GAGGAGGCTG
CTGCAGATGA AAGAAGAAGC AACAATGGCC AACGAAGCAC TGATGCGGTC TGAGGAGACA
GCTGACCTGT TGGCTGAAAA GGCCCAGATC ACCGAGGAGG AGGCAAAACT TCTGGCCCAG
AAGGCCGCAG AGGCTGAGCA GGAAATGCAG CGCATCAAGG CCACAGCGAT TCGCACGGAG
GAGGAGAAGC GCCTGATGGA GCAGAAGGTG CTGGAAGCCG AGGTGCTGGC ACTGAAGATG
GCTGAGGAGT CAGAGAGGAG GGCCAAAGAG GCAGATCAGC TGAAGCAGGA CCTGCAGGAA
GCACGCGAGG CGGAGCGAAG AGCCAAGCAG AAGCTCCTGG AGATTGCCAC CAAGCCCACG
TACCCGCCCA TGAACCCAAT TCCAGCACCG TTGCCTCCTG ACATACCAAG CTTCAACCTC
ATTGGTGACA GCCTGTCTTT CGACTTCAAA GATACTGACA TGAAGCGGCT TTCCATGGAG
ATAGAGAAAG AAAAAGTGGA ATACATGGAA AAGAGCAAGC ATCTGCAGGA GCAGCTCAAT
GAACTCAAGA CAGAAATCGA GGCCTTGAAA CTGAAAGAGA GGGAGACAGC TCTGGATATT
CTGCACAATG AGAACTCCGA CAGGGGTGGC AGCAGCAAGC ACAATACCAT TAAAAAGCCT
CAAGCCCAAG GCAGAAGACC TATCTGCATT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_861970.1
CDS444..2216
Misc Feature(1)357..359(+)
Misc Feature(2)510..1109(+)
Misc Feature(3)519..749(+)
Misc Feature(4)783..1085(+)
Misc Feature(5)1089..1379(+)
Misc Feature(6)1215..1355(+)
Misc Feature(7)1215..1355(+)
Misc Feature(8)1323..1337(+)
Misc Feature(9)1332..1379(+)
Misc Feature(10)1404..2066(+)
Misc Feature(11)1482..2180(+)
Misc Feature(12)1995..1997(+)
Misc Feature(13)1995..1997(+)
Misc Feature(14)1995..1997(+)
Exon (1)1..557
Gene:NF2
Gene Synonym:
Exon (2)558..683
Gene:NF2
Gene Synonym:
Exon (3)684..806
Gene:NF2
Gene Synonym:
Exon (4)807..890
Gene:NF2
Gene Synonym:
Exon (5)891..959
Gene:NF2
Gene Synonym:
Exon (6)960..1042
Gene:NF2
Gene Synonym:
Exon (7)1043..1118
Gene:NF2
Gene Synonym:
Exon (8)1119..1253
Gene:NF2
Gene Synonym:
Exon (9)1254..1328
Gene:NF2
Gene Synonym:
Exon (10)1329..1442
Gene:NF2
Gene Synonym:
Exon (11)1443..1565
Gene:NF2
Gene Synonym:
Exon (12)1566..1783
Gene:NF2
Gene Synonym:
Exon (13)1784..1889
Gene:NF2
Gene Synonym:
Exon (14)1890..2017
Gene:NF2
Gene Synonym:
Exon (15)2018..2180
Gene:NF2
Gene Synonym:
Exon (16)2181..2240
Gene:NF2
Gene Synonym:
Exon (17)2241..6089
Gene:NF2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_181832
Organism Homo sapiens (human)
Definition Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_181832

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGATGAAG
TGGAAAGGGA AGGACCTCTT TGATTTGGTG TGCCGGACTC TGGGGCTCCG AGAAACCTGG
TTCTTTGGAC TGCAGTACAC AATCAAGGAC ACAGTGGCCT GGCTCAAAAT GGACAAGAAG
GTACTGGATC ATGATGTTTC AAAGGAAGAA CCAGTCACCT TTCACTTCTT GGCCAAATTT
TATCCTGAGA ATGCTGAAGA GGAGCTGGTT CAGGAGATCA CACAACATTT ATTCTTCTTA
CAGGTAAAGA AGCAGATTTT AGATGAAAAG ATCTACTGCC CTCCTGAGGC TTCTGTGCTC
CTGGCTTCTT ACGCCGTCCA GGCCAAGTAT GGTGACTACG ACCCCAGTGT TCACAAGCGG
GGATTTTTGG CCCAAGAGGA ATTGCTTCCA AAAAGGGTAA TAAATCTGTA TCAGATGACT
CCGGAAATGT GGGAGGAGAG AATTACTGCT TGGTACGCAG AGCACCGAGG CCGAGCCAGG
GATGAAGCTG AAATGGAATA TCTGAAGATA GCTCAGGACC TGGAGATGTA CGGTGTGAAC
TACTTTGCAA TCCGGAATAA AAAGGGCACA GAGCTGCTGC TTGGAGTGGA TGCCCTGGGG
CTTCACATTT ATGACCCTGA GAACAGACTG ACCCCCAAGA TCTCCTTCCC GTGGAATGAA
ATCCGAAACA TCTCGTACAG TGACAAGGAG TTTACTATTA AACCACTGGA TAAGAAAATT
GATGTCTTCA AGTTTAACTC CTCAAAGCTT CGTGTTAATA AGCTGATTCT CCAGCTATGT
ATCGGGAACC ATGATCTATT TATGAGGAGA AGGAAAGCCG ATTCTTTGGA AGTTCAGCAG
ATGAAAGCCC AGGCCAGGGA GGAGAAGGCT AGAAAGCAGA TGGAGCGGCA GCGCCTCGCT
CGAGAGAAGC AGATGAGGGA GGAGGCTGAA CGCACGAGGG ATGAGTTGGA GAGGAGGCTG
CTGCAGATGA AAGAAGAAGC AACAATGGCC AACGAAGCAC TGATGCGGTC TGAGGAGACA
GCTGACCTGT TGGCTGAAAA GGCCCAGATC ACCGAGGAGG AGGCAAAACT TCTGGCCCAG
AAGGCCGCAG AGGCTGAGCA GGAAATGCAG CGCATCAAGG CCACAGCGAT TCGCACGGAG
GAGGAGAAGC GCCTGATGGA GCAGAAGGTG CTGGAAGCCG AGGTGCTGGC ACTGAAGATG
GCTGAGGAGT CAGAGAGGAG GGCCAAAGAG GCAGATCAGC TGAAGCAGGA CCTGCAGGAA
GCACGCGAGG CGGAGCGAAG AGCCAAGCAG AAGCTCCTGG AGATTGCCAC CAAGCCCACG
TACCCGCCCA TGAACCCAAT TCCAGCACCG TTGCCTCCTG ACATACCAAG CTTCAACCTC
ATTGGTGACA GCCTGTCTTT CGACTTCAAA GATACTGACA TGAAGCGGCT TTCCATGGAG
ATAGAGAAAG AAAAAGTGGA ATACATGGAA AAGAGCAAGC ATCTGCAGGA GCAGCTCAAT
GAACTCAAGA CAGAAATCGA GGCCTTGAAA CTGAAAGAGA GGGAGACAGC TCTGGATATT
CTGCACAATG AGAACTCCGA CAGGGGTGGC AGCAGCAAGC ACAATACCAT TAAAAAGCCT
CAAGCCCAAG GCAGAAGACC TATCTGCATT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16399
Clone ID Related Accession (Same CDS sequence) NM_181833
Accession Version NM_181833.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 498bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product merlin isoform 8
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN419318.1, CF138178.1, AF122827.1, AC004882.3 and BC071653.1. On Dec 20, 2007 this sequence version replaced gi:32967267. Transcript Variant: This variant (9) lacks several alternate in-frame exons, compared to variant 1. The resulting protein (isoform 8, also referred to as isoform Mer162) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF122827.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGATGAAG
TGGAAAGGGA AGGACCTCTT TGATTTGGTG TGCCGGACTC TGGGGCTCCG AGAAACCTGG
TTCTTTGGAC TGCAGTACAC AATCAAGGAC ACAGTGGCCT GGCTCAAAAT GGACAAGAAG
GTACTGGATC ATGATGTTTC AAAGGAAGAA CCAGTCACCT TTCACTTCTT GGCCAAATTT
TATCCTGAGA ATGCTGAAGA GGAGCTGGTT CAGGAGATCA CACAACATTT ATTCTTCTTA
CAGGTAAAGA AGCAGATTTT AGATGAAAAG ATCTACTGCC CTCCTGAGGC TTCTGTGCTC
CTGGCTTCTT ACGCCGTCCA GGCCAAGCTC ACCTTGCAGA GCGCCAAGTC CCGAGTGGCC
TTCTTTGAAG AGCTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_861971.1
CDS444..941
Misc Feature(1)357..359(+)
Misc Feature(2)510..>893(+)
Misc Feature(3)519..749(+)
Misc Feature(4)783..>893(+)
Exon (1)1..557
Gene:NF2
Gene Synonym:
Exon (2)558..683
Gene:NF2
Gene Synonym:
Exon (3)684..806
Gene:NF2
Gene Synonym:
Exon (4)807..890
Gene:NF2
Gene Synonym:
Exon (5)891..4739
Gene:NF2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_181833
Organism Homo sapiens (human)
Definition Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_181833

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGATGAAG
TGGAAAGGGA AGGACCTCTT TGATTTGGTG TGCCGGACTC TGGGGCTCCG AGAAACCTGG
TTCTTTGGAC TGCAGTACAC AATCAAGGAC ACAGTGGCCT GGCTCAAAAT GGACAAGAAG
GTACTGGATC ATGATGTTTC AAAGGAAGAA CCAGTCACCT TTCACTTCTT GGCCAAATTT
TATCCTGAGA ATGCTGAAGA GGAGCTGGTT CAGGAGATCA CACAACATTT ATTCTTCTTA
CAGGTAAAGA AGCAGATTTT AGATGAAAAG ATCTACTGCC CTCCTGAGGC TTCTGTGCTC
CTGGCTTCTT ACGCCGTCCA GGCCAAGCTC ACCTTGCAGA GCGCCAAGTC CCGAGTGGCC
TTCTTTGAAG AGCTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16401
Clone ID Related Accession (Same CDS sequence) NM_016418 , NM_181825 , NM_181832
Accession Version NM_181825.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1773bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product merlin isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN419318.1 and AF369701.1. On Dec 20, 2007 this sequence version replaced gi:32967253. Transcript Variant: This variant (12) includes an alternate exon and differs in the 3' UTR, compared to variant 1. The resulting protein (isoform 2), one of the two predominant isoforms, has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF369701.1, AF369662.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGATGAAG
TGGAAAGGGA AGGACCTCTT TGATTTGGTG TGCCGGACTC TGGGGCTCCG AGAAACCTGG
TTCTTTGGAC TGCAGTACAC AATCAAGGAC ACAGTGGCCT GGCTCAAAAT GGACAAGAAG
GTACTGGATC ATGATGTTTC AAAGGAAGAA CCAGTCACCT TTCACTTCTT GGCCAAATTT
TATCCTGAGA ATGCTGAAGA GGAGCTGGTT CAGGAGATCA CACAACATTT ATTCTTCTTA
CAGGTAAAGA AGCAGATTTT AGATGAAAAG ATCTACTGCC CTCCTGAGGC TTCTGTGCTC
CTGGCTTCTT ACGCCGTCCA GGCCAAGTAT GGTGACTACG ACCCCAGTGT TCACAAGCGG
GGATTTTTGG CCCAAGAGGA ATTGCTTCCA AAAAGGGTAA TAAATCTGTA TCAGATGACT
CCGGAAATGT GGGAGGAGAG AATTACTGCT TGGTACGCAG AGCACCGAGG CCGAGCCAGG
GATGAAGCTG AAATGGAATA TCTGAAGATA GCTCAGGACC TGGAGATGTA CGGTGTGAAC
TACTTTGCAA TCCGGAATAA AAAGGGCACA GAGCTGCTGC TTGGAGTGGA TGCCCTGGGG
CTTCACATTT ATGACCCTGA GAACAGACTG ACCCCCAAGA TCTCCTTCCC GTGGAATGAA
ATCCGAAACA TCTCGTACAG TGACAAGGAG TTTACTATTA AACCACTGGA TAAGAAAATT
GATGTCTTCA AGTTTAACTC CTCAAAGCTT CGTGTTAATA AGCTGATTCT CCAGCTATGT
ATCGGGAACC ATGATCTATT TATGAGGAGA AGGAAAGCCG ATTCTTTGGA AGTTCAGCAG
ATGAAAGCCC AGGCCAGGGA GGAGAAGGCT AGAAAGCAGA TGGAGCGGCA GCGCCTCGCT
CGAGAGAAGC AGATGAGGGA GGAGGCTGAA CGCACGAGGG ATGAGTTGGA GAGGAGGCTG
CTGCAGATGA AAGAAGAAGC AACAATGGCC AACGAAGCAC TGATGCGGTC TGAGGAGACA
GCTGACCTGT TGGCTGAAAA GGCCCAGATC ACCGAGGAGG AGGCAAAACT TCTGGCCCAG
AAGGCCGCAG AGGCTGAGCA GGAAATGCAG CGCATCAAGG CCACAGCGAT TCGCACGGAG
GAGGAGAAGC GCCTGATGGA GCAGAAGGTG CTGGAAGCCG AGGTGCTGGC ACTGAAGATG
GCTGAGGAGT CAGAGAGGAG GGCCAAAGAG GCAGATCAGC TGAAGCAGGA CCTGCAGGAA
GCACGCGAGG CGGAGCGAAG AGCCAAGCAG AAGCTCCTGG AGATTGCCAC CAAGCCCACG
TACCCGCCCA TGAACCCAAT TCCAGCACCG TTGCCTCCTG ACATACCAAG CTTCAACCTC
ATTGGTGACA GCCTGTCTTT CGACTTCAAA GATACTGACA TGAAGCGGCT TTCCATGGAG
ATAGAGAAAG AAAAAGTGGA ATACATGGAA AAGAGCAAGC ATCTGCAGGA GCAGCTCAAT
GAACTCAAGA CAGAAATCGA GGCCTTGAAA CTGAAAGAGA GGGAGACAGC TCTGGATATT
CTGCACAATG AGAACTCCGA CAGGGGTGGC AGCAGCAAGC ACAATACCAT TAAAAAGCCT
CAAGCCCAAG GCAGAAGACC TATCTGCATT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_861546.1
CDS444..2216
Misc Feature(1)357..359(+)
Misc Feature(2)510..1109(+)
Misc Feature(3)519..749(+)
Misc Feature(4)783..1085(+)
Misc Feature(5)1089..1379(+)
Misc Feature(6)1215..1355(+)
Misc Feature(7)1215..1355(+)
Misc Feature(8)1323..1337(+)
Misc Feature(9)1332..1379(+)
Misc Feature(10)1404..2066(+)
Misc Feature(11)1482..2180(+)
Misc Feature(12)1995..1997(+)
Misc Feature(13)1995..1997(+)
Misc Feature(14)1995..1997(+)
Exon (1)1..557
Gene:NF2
Gene Synonym:
Exon (2)558..683
Gene:NF2
Gene Synonym:
Exon (3)684..806
Gene:NF2
Gene Synonym:
Exon (4)807..890
Gene:NF2
Gene Synonym:
Exon (5)891..959
Gene:NF2
Gene Synonym:
Exon (6)960..1042
Gene:NF2
Gene Synonym:
Exon (7)1043..1118
Gene:NF2
Gene Synonym:
Exon (8)1119..1253
Gene:NF2
Gene Synonym:
Exon (9)1254..1328
Gene:NF2
Gene Synonym:
Exon (10)1329..1442
Gene:NF2
Gene Synonym:
Exon (11)1443..1565
Gene:NF2
Gene Synonym:
Exon (12)1566..1783
Gene:NF2
Gene Synonym:
Exon (13)1784..1889
Gene:NF2
Gene Synonym:
Exon (14)1890..2017
Gene:NF2
Gene Synonym:
Exon (15)2018..2180
Gene:NF2
Gene Synonym:
Exon (16)2181..3076
Gene:NF2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_181825
Organism Homo sapiens (human)
Definition Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_181825

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGATGAAG
TGGAAAGGGA AGGACCTCTT TGATTTGGTG TGCCGGACTC TGGGGCTCCG AGAAACCTGG
TTCTTTGGAC TGCAGTACAC AATCAAGGAC ACAGTGGCCT GGCTCAAAAT GGACAAGAAG
GTACTGGATC ATGATGTTTC AAAGGAAGAA CCAGTCACCT TTCACTTCTT GGCCAAATTT
TATCCTGAGA ATGCTGAAGA GGAGCTGGTT CAGGAGATCA CACAACATTT ATTCTTCTTA
CAGGTAAAGA AGCAGATTTT AGATGAAAAG ATCTACTGCC CTCCTGAGGC TTCTGTGCTC
CTGGCTTCTT ACGCCGTCCA GGCCAAGTAT GGTGACTACG ACCCCAGTGT TCACAAGCGG
GGATTTTTGG CCCAAGAGGA ATTGCTTCCA AAAAGGGTAA TAAATCTGTA TCAGATGACT
CCGGAAATGT GGGAGGAGAG AATTACTGCT TGGTACGCAG AGCACCGAGG CCGAGCCAGG
GATGAAGCTG AAATGGAATA TCTGAAGATA GCTCAGGACC TGGAGATGTA CGGTGTGAAC
TACTTTGCAA TCCGGAATAA AAAGGGCACA GAGCTGCTGC TTGGAGTGGA TGCCCTGGGG
CTTCACATTT ATGACCCTGA GAACAGACTG ACCCCCAAGA TCTCCTTCCC GTGGAATGAA
ATCCGAAACA TCTCGTACAG TGACAAGGAG TTTACTATTA AACCACTGGA TAAGAAAATT
GATGTCTTCA AGTTTAACTC CTCAAAGCTT CGTGTTAATA AGCTGATTCT CCAGCTATGT
ATCGGGAACC ATGATCTATT TATGAGGAGA AGGAAAGCCG ATTCTTTGGA AGTTCAGCAG
ATGAAAGCCC AGGCCAGGGA GGAGAAGGCT AGAAAGCAGA TGGAGCGGCA GCGCCTCGCT
CGAGAGAAGC AGATGAGGGA GGAGGCTGAA CGCACGAGGG ATGAGTTGGA GAGGAGGCTG
CTGCAGATGA AAGAAGAAGC AACAATGGCC AACGAAGCAC TGATGCGGTC TGAGGAGACA
GCTGACCTGT TGGCTGAAAA GGCCCAGATC ACCGAGGAGG AGGCAAAACT TCTGGCCCAG
AAGGCCGCAG AGGCTGAGCA GGAAATGCAG CGCATCAAGG CCACAGCGAT TCGCACGGAG
GAGGAGAAGC GCCTGATGGA GCAGAAGGTG CTGGAAGCCG AGGTGCTGGC ACTGAAGATG
GCTGAGGAGT CAGAGAGGAG GGCCAAAGAG GCAGATCAGC TGAAGCAGGA CCTGCAGGAA
GCACGCGAGG CGGAGCGAAG AGCCAAGCAG AAGCTCCTGG AGATTGCCAC CAAGCCCACG
TACCCGCCCA TGAACCCAAT TCCAGCACCG TTGCCTCCTG ACATACCAAG CTTCAACCTC
ATTGGTGACA GCCTGTCTTT CGACTTCAAA GATACTGACA TGAAGCGGCT TTCCATGGAG
ATAGAGAAAG AAAAAGTGGA ATACATGGAA AAGAGCAAGC ATCTGCAGGA GCAGCTCAAT
GAACTCAAGA CAGAAATCGA GGCCTTGAAA CTGAAAGAGA GGGAGACAGC TCTGGATATT
CTGCACAATG AGAACTCCGA CAGGGGTGGC AGCAGCAAGC ACAATACCAT TAAAAAGCCT
CAAGCCCAAG GCAGAAGACC TATCTGCATT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16489
Clone ID Related Accession (Same CDS sequence) NM_181830 , NM_181831
Accession Version NM_181831.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1524bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product merlin isoform 7
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN419318.1, AF369657.1 and BC003112.1. On Dec 20, 2007 this sequence version replaced gi:32967513. Transcript Variant: This variant (13) lacks two alternate in-frame exons in the 5' coding region and differs in the 3' coding region and UTR, compared to variant 1. The resulting protein (isoform 7, also referred to as isoform delE2/3) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003112.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGGTAAAG
AAGCAGATTT TAGATGAAAA GATCTACTGC CCTCCTGAGG CTTCTGTGCT CCTGGCTTCT
TACGCCGTCC AGGCCAAGTA TGGTGACTAC GACCCCAGTG TTCACAAGCG GGGATTTTTG
GCCCAAGAGG AATTGCTTCC AAAAAGGGTA ATAAATCTGT ATCAGATGAC TCCGGAAATG
TGGGAGGAGA GAATTACTGC TTGGTACGCA GAGCACCGAG GCCGAGCCAG GGATGAAGCT
GAAATGGAAT ATCTGAAGAT AGCTCAGGAC CTGGAGATGT ACGGTGTGAA CTACTTTGCA
ATCCGGAATA AAAAGGGCAC AGAGCTGCTG CTTGGAGTGG ATGCCCTGGG GCTTCACATT
TATGACCCTG AGAACAGACT GACCCCCAAG ATCTCCTTCC CGTGGAATGA AATCCGAAAC
ATCTCGTACA GTGACAAGGA GTTTACTATT AAACCACTGG ATAAGAAAAT TGATGTCTTC
AAGTTTAACT CCTCAAAGCT TCGTGTTAAT AAGCTGATTC TCCAGCTATG TATCGGGAAC
CATGATCTAT TTATGAGGAG AAGGAAAGCC GATTCTTTGG AAGTTCAGCA GATGAAAGCC
CAGGCCAGGG AGGAGAAGGC TAGAAAGCAG ATGGAGCGGC AGCGCCTCGC TCGAGAGAAG
CAGATGAGGG AGGAGGCTGA ACGCACGAGG GATGAGTTGG AGAGGAGGCT GCTGCAGATG
AAAGAAGAAG CAACAATGGC CAACGAAGCA CTGATGCGGT CTGAGGAGAC AGCTGACCTG
TTGGCTGAAA AGGCCCAGAT CACCGAGGAG GAGGCAAAAC TTCTGGCCCA GAAGGCCGCA
GAGGCTGAGC AGGAAATGCA GCGCATCAAG GCCACAGCGA TTCGCACGGA GGAGGAGAAG
CGCCTGATGG AGCAGAAGGT GCTGGAAGCC GAGGTGCTGG CACTGAAGAT GGCTGAGGAG
TCAGAGAGGA GGGCCAAAGA GGCAGATCAG CTGAAGCAGG ACCTGCAGGA AGCACGCGAG
GCGGAGCGAA GAGCCAAGCA GAAGCTCCTG GAGATTGCCA CCAAGCCCAC GTACCCGCCC
ATGAACCCAA TTCCAGCACC GTTGCCTCCT GACATACCAA GCTTCAACCT CATTGGTGAC
AGCCTGTCTT TCGACTTCAA AGATACTGAC ATGAAGCGGC TTTCCATGGA GATAGAGAAA
GAAAAAGTGG AATACATGGA AAAGAGCAAG CATCTGCAGG AGCAGCTCAA TGAACTCAAG
ACAGAAATCG AGGCCTTGAA ACTGAAAGAG AGGGAGACAG CTCTGGATAT TCTGCACAAT
GAGAACTCCG ACAGGGGTGG CAGCAGCAAG CACAATACCA TTAAAAAGCC TCAAGCCCAA
GGCAGAAGAC CTATCTGCAT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_861969.1
CDS444..1967
Misc Feature(1)357..359(+)
Misc Feature(2)552..860(+)
Misc Feature(3)558..836(+)
Misc Feature(4)840..1130(+)
Misc Feature(5)966..1106(+)
Misc Feature(6)966..1106(+)
Misc Feature(7)1074..1088(+)
Misc Feature(8)1083..1130(+)
Misc Feature(9)1155..1817(+)
Misc Feature(10)1233..1931(+)
Misc Feature(11)1746..1748(+)
Misc Feature(12)1746..1748(+)
Exon (1)1..557
Gene:NF2
Gene Synonym:
Exon (2)558..641
Gene:NF2
Gene Synonym:
Exon (3)642..710
Gene:NF2
Gene Synonym:
Exon (4)711..793
Gene:NF2
Gene Synonym:
Exon (5)794..869
Gene:NF2
Gene Synonym:
Exon (6)870..1004
Gene:NF2
Gene Synonym:
Exon (7)1005..1079
Gene:NF2
Gene Synonym:
Exon (8)1080..1193
Gene:NF2
Gene Synonym:
Exon (9)1194..1316
Gene:NF2
Gene Synonym:
Exon (10)1317..1534
Gene:NF2
Gene Synonym:
Exon (11)1535..1640
Gene:NF2
Gene Synonym:
Exon (12)1641..1768
Gene:NF2
Gene Synonym:
Exon (13)1769..1931
Gene:NF2
Gene Synonym:
Exon (14)1932..1976
Gene:NF2
Gene Synonym:
Exon (15)1977..2191
Gene:NF2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_181831
Organism Homo sapiens (human)
Definition Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 13, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_181831

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGGTAAAG
AAGCAGATTT TAGATGAAAA GATCTACTGC CCTCCTGAGG CTTCTGTGCT CCTGGCTTCT
TACGCCGTCC AGGCCAAGTA TGGTGACTAC GACCCCAGTG TTCACAAGCG GGGATTTTTG
GCCCAAGAGG AATTGCTTCC AAAAAGGGTA ATAAATCTGT ATCAGATGAC TCCGGAAATG
TGGGAGGAGA GAATTACTGC TTGGTACGCA GAGCACCGAG GCCGAGCCAG GGATGAAGCT
GAAATGGAAT ATCTGAAGAT AGCTCAGGAC CTGGAGATGT ACGGTGTGAA CTACTTTGCA
ATCCGGAATA AAAAGGGCAC AGAGCTGCTG CTTGGAGTGG ATGCCCTGGG GCTTCACATT
TATGACCCTG AGAACAGACT GACCCCCAAG ATCTCCTTCC CGTGGAATGA AATCCGAAAC
ATCTCGTACA GTGACAAGGA GTTTACTATT AAACCACTGG ATAAGAAAAT TGATGTCTTC
AAGTTTAACT CCTCAAAGCT TCGTGTTAAT AAGCTGATTC TCCAGCTATG TATCGGGAAC
CATGATCTAT TTATGAGGAG AAGGAAAGCC GATTCTTTGG AAGTTCAGCA GATGAAAGCC
CAGGCCAGGG AGGAGAAGGC TAGAAAGCAG ATGGAGCGGC AGCGCCTCGC TCGAGAGAAG
CAGATGAGGG AGGAGGCTGA ACGCACGAGG GATGAGTTGG AGAGGAGGCT GCTGCAGATG
AAAGAAGAAG CAACAATGGC CAACGAAGCA CTGATGCGGT CTGAGGAGAC AGCTGACCTG
TTGGCTGAAA AGGCCCAGAT CACCGAGGAG GAGGCAAAAC TTCTGGCCCA GAAGGCCGCA
GAGGCTGAGC AGGAAATGCA GCGCATCAAG GCCACAGCGA TTCGCACGGA GGAGGAGAAG
CGCCTGATGG AGCAGAAGGT GCTGGAAGCC GAGGTGCTGG CACTGAAGAT GGCTGAGGAG
TCAGAGAGGA GGGCCAAAGA GGCAGATCAG CTGAAGCAGG ACCTGCAGGA AGCACGCGAG
GCGGAGCGAA GAGCCAAGCA GAAGCTCCTG GAGATTGCCA CCAAGCCCAC GTACCCGCCC
ATGAACCCAA TTCCAGCACC GTTGCCTCCT GACATACCAA GCTTCAACCT CATTGGTGAC
AGCCTGTCTT TCGACTTCAA AGATACTGAC ATGAAGCGGC TTTCCATGGA GATAGAGAAA
GAAAAAGTGG AATACATGGA AAAGAGCAAG CATCTGCAGG AGCAGCTCAA TGAACTCAAG
ACAGAAATCG AGGCCTTGAA ACTGAAAGAG AGGGAGACAG CTCTGGATAT TCTGCACAAT
GAGAACTCCG ACAGGGGTGG CAGCAGCAAG CACAATACCA TTAAAAAGCC TCAAGCCCAA
GGCAGAAGAC CTATCTGCAT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Neuronal merlin influences ERBB2 receptor expression on Schwann cells through neuregulin 1 type III signalling
Brain 137 (PT 2), 420-432 (2014)
Schulz A, Kyselyova A, Baader SL, Jung MJ, Zoch A, Mautner VF, Hagel C and Morrison H.


book

Hearing and facial function outcomes for neurofibromatosis 2 clinical trials
Neurology 81 (21 SUPPL 1), S25-S32 (2013)
Plotkin SR, Ardern-Holmes SL, Barker FG 2nd, Blakeley JO, Evans DG, Ferner RE, Hadlock TA and Halpin C.


book

NF2 genetic alterations in sporadic vestibular schwannomas: clinical implications
Otol. Neurotol. 34 (7), 1355-1361 (2013)
Lassaletta L, Torres-Martin M, Pena-Granero C, Roda JM, Santa-Cruz-Ruiz S, Castresana JS, Gavilan J and Rey JA.


book

Merlin, the NF2 gene product
Pathol. Oncol. Res. 19 (3), 365-373 (2013)
Pecina-Slaus N.


book

Association between mutation of the NF2 gene and monosomy 22 in menopausal women with sporadic meningiomas
BMC Med. Genet. 14, 114 (2013)
Tabernero M, Jara-Acevedo M, Nieto AB, Caballero AR, Otero A, Sousa P, Goncalves J, Domingues PH and Orfao A.


book

Neurofibromatosis type 2 protein co-localizes with elements of the cytoskeleton
Am. J. Pathol. 147 (5), 1339-1349 (1995)
den Bakker MA, Tascilar M, Riegman PH, Hekman AC, Boersma W, Janssen PJ, de Jong TA, Hendriks W, van der Kwast TH and Zwarthoff EC.


book

Diagnostic issues in a family with late onset type 2 neurofibromatosis
J. Med. Genet. 32 (6), 470-474 (1995)
Evans DG, Bourn D, Wallace A, Ramsden RT, Mitchell JD and Strachan T.


book

Neurofibromatosis 2 and neurilemmomatosis gene are identical
J. Invest. Dermatol. 104 (1), 74-77 (1995)
Honda M, Arai E, Sawada S, Ohta A and Niimura M.


book

Neurofibromatosis 2
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Evans,D.G.


book

Constitutional translocation t(4;22) (q12;q12.2) associated with neurofibromatosis type 2
Am. J. Med. Genet. 44 (2), 163-167 (1992)
Arai E, Ikeuchi T, Karasawa S, Tamura A, Yamamoto K, Kida M, Ichimura K, Yuasa Y and Tonomura A.


 
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