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NF2 neurofibromin 2 (merlin) [Homo sapiens (human)]

Gene Symbol NF2
Entrez Gene ID 4771
Full Name neurofibromin 2 (merlin)
Synonyms ACN, BANF, SCH
General protein information
Preferred Names
merlin
Names
merlin
schwannomin
schwannomerlin
neurofibromin-2
moesin-ezrin-radixin like
moesin-ezrin-radixin-like protein
moesin-ezrin-radizin-like protein
neurofibromin 2 (bilateral acoustic neuroma)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

22

22q12.2

Summary This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

607379

Disorder Html: Neurofibromatosis, type 2, 101000 (3); Meningioma, NF2-related,





The following NF2 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NF2 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18888 NM_000268 Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu16401 NM_016418 Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu16463 NM_181828 Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu16365 NM_181829 Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu16489 NM_181830 Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$439
OHu16401 NM_181832 Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu16399 NM_181833 Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99
OHu16401 NM_181825 Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu16489 NM_181831 Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$439

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu18888
Accession Version NM_000268.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1788bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product merlin isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN419318.1, AF369657.1, AC004882.3 and BC071653.1. This sequence is a reference standard in the RefSeqGene project. On Dec 20, 2007 this sequence version replaced gi:32967273. Summary: This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) encodes the longer predominant isoform (1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF369657.1, BC020257.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGATGAAG
TGGAAAGGGA AGGACCTCTT TGATTTGGTG TGCCGGACTC TGGGGCTCCG AGAAACCTGG
TTCTTTGGAC TGCAGTACAC AATCAAGGAC ACAGTGGCCT GGCTCAAAAT GGACAAGAAG
GTACTGGATC ATGATGTTTC AAAGGAAGAA CCAGTCACCT TTCACTTCTT GGCCAAATTT
TATCCTGAGA ATGCTGAAGA GGAGCTGGTT CAGGAGATCA CACAACATTT ATTCTTCTTA
CAGGTAAAGA AGCAGATTTT AGATGAAAAG ATCTACTGCC CTCCTGAGGC TTCTGTGCTC
CTGGCTTCTT ACGCCGTCCA GGCCAAGTAT GGTGACTACG ACCCCAGTGT TCACAAGCGG
GGATTTTTGG CCCAAGAGGA ATTGCTTCCA AAAAGGGTAA TAAATCTGTA TCAGATGACT
CCGGAAATGT GGGAGGAGAG AATTACTGCT TGGTACGCAG AGCACCGAGG CCGAGCCAGG
GATGAAGCTG AAATGGAATA TCTGAAGATA GCTCAGGACC TGGAGATGTA CGGTGTGAAC
TACTTTGCAA TCCGGAATAA AAAGGGCACA GAGCTGCTGC TTGGAGTGGA TGCCCTGGGG
CTTCACATTT ATGACCCTGA GAACAGACTG ACCCCCAAGA TCTCCTTCCC GTGGAATGAA
ATCCGAAACA TCTCGTACAG TGACAAGGAG TTTACTATTA AACCACTGGA TAAGAAAATT
GATGTCTTCA AGTTTAACTC CTCAAAGCTT CGTGTTAATA AGCTGATTCT CCAGCTATGT
ATCGGGAACC ATGATCTATT TATGAGGAGA AGGAAAGCCG ATTCTTTGGA AGTTCAGCAG
ATGAAAGCCC AGGCCAGGGA GGAGAAGGCT AGAAAGCAGA TGGAGCGGCA GCGCCTCGCT
CGAGAGAAGC AGATGAGGGA GGAGGCTGAA CGCACGAGGG ATGAGTTGGA GAGGAGGCTG
CTGCAGATGA AAGAAGAAGC AACAATGGCC AACGAAGCAC TGATGCGGTC TGAGGAGACA
GCTGACCTGT TGGCTGAAAA GGCCCAGATC ACCGAGGAGG AGGCAAAACT TCTGGCCCAG
AAGGCCGCAG AGGCTGAGCA GGAAATGCAG CGCATCAAGG CCACAGCGAT TCGCACGGAG
GAGGAGAAGC GCCTGATGGA GCAGAAGGTG CTGGAAGCCG AGGTGCTGGC ACTGAAGATG
GCTGAGGAGT CAGAGAGGAG GGCCAAAGAG GCAGATCAGC TGAAGCAGGA CCTGCAGGAA
GCACGCGAGG CGGAGCGAAG AGCCAAGCAG AAGCTCCTGG AGATTGCCAC CAAGCCCACG
TACCCGCCCA TGAACCCAAT TCCAGCACCG TTGCCTCCTG ACATACCAAG CTTCAACCTC
ATTGGTGACA GCCTGTCTTT CGACTTCAAA GATACTGACA TGAAGCGGCT TTCCATGGAG
ATAGAGAAAG AAAAAGTGGA ATACATGGAA AAGAGCAAGC ATCTGCAGGA GCAGCTCAAT
GAACTCAAGA CAGAAATCGA GGCCTTGAAA CTGAAAGAGA GGGAGACAGC TCTGGATATT
CTGCACAATG AGAACTCCGA CAGGGGTGGC AGCAGCAAGC ACAATACCAT TAAAAAGCTC
ACCTTGCAGA GCGCCAAGTC CCGAGTGGCC TTCTTTGAAG AGCTCTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000259.1
CDS444..2231
Misc Feature(1)357..359(+)
Misc Feature(2)510..1109(+)
Misc Feature(3)519..749(+)
Misc Feature(4)783..1085(+)
Misc Feature(5)1089..1379(+)
Misc Feature(6)1215..1355(+)
Misc Feature(7)1215..1355(+)
Misc Feature(8)1323..1337(+)
Misc Feature(9)1332..1379(+)
Misc Feature(10)1404..2066(+)
Misc Feature(11)1482..2228(+)
Misc Feature(12)1995..1997(+)
Misc Feature(13)1995..1997(+)
Misc Feature(14)1995..1997(+)
Exon (1)1..557
Gene:NF2
Gene Synonym:
Exon (2)558..683
Gene:NF2
Gene Synonym:
Exon (3)684..806
Gene:NF2
Gene Synonym:
Exon (4)807..890
Gene:NF2
Gene Synonym:
Exon (5)891..959
Gene:NF2
Gene Synonym:
Exon (6)960..1042
Gene:NF2
Gene Synonym:
Exon (7)1043..1118
Gene:NF2
Gene Synonym:
Exon (8)1119..1253
Gene:NF2
Gene Synonym:
Exon (9)1254..1328
Gene:NF2
Gene Synonym:
Exon (10)1329..1442
Gene:NF2
Gene Synonym:
Exon (11)1443..1565
Gene:NF2
Gene Synonym:
Exon (12)1566..1783
Gene:NF2
Gene Synonym:
Exon (13)1784..1889
Gene:NF2
Gene Synonym:
Exon (14)1890..2017
Gene:NF2
Gene Synonym:
Exon (15)2018..2180
Gene:NF2
Gene Synonym:
Exon (16)2181..6029
Gene:NF2
Gene Synonym:
Translation
Position Chain Variation Link
40 40 c, t dbSNP:570135699
55 55 c, t dbSNP:539283977
56 56 a, g dbSNP:777179356
103 103 a, c dbSNP:571842282
114 114 g, t dbSNP:554177413
198 198 c, g dbSNP:56733822
199 199 c, g dbSNP:536438675
208 208 c, t dbSNP:555236806
240 240 a, c dbSNP:1800539
334 334 c, g dbSNP:1800540
341 341 c, g dbSNP:1042230
360 360 c, g dbSNP:558960493
396 396 a, c dbSNP:368022063
398 398 a, g dbSNP:753401671
405 405 c, g dbSNP:3181682
416 416 c, t dbSNP:763602804
425 425 c, t dbSNP:577180052
426 426 a, g dbSNP:201591536
427 427 a, g dbSNP:748043566
430 430 c, t dbSNP:758236169
435 435 c, t dbSNP:777578586
436 436 c, t dbSNP:746741839
442 442 c, g dbSNP:559386105
455 455 c, t dbSNP:144477078
466 466 c, g dbSNP:775564806
467 467 a, c dbSNP:749452887
479 479 c, t dbSNP:371800843
491 491 a, c, g dbSNP:774973059
500 500 c, g dbSNP:541641232
514 514 c, t dbSNP:773714780
515 515 c, g dbSNP:761062232
532 532 a, g dbSNP:563168478
537 537 a, g dbSNP:373337083
543 543 c, g dbSNP:753425376
550 550 a, g dbSNP:372279458
558 558 a, g dbSNP:761188569
568 568 -, g dbSNP:587776564
576 576 g, t dbSNP:370147621
578 578 c, t dbSNP:752664255
588 588 a, t dbSNP:759129920
597 597 c, t dbSNP:764901064
603 603 c, t dbSNP:752220604
604 604 g, t dbSNP:762440698
606 606 a, g dbSNP:764089682
612 612 c, t dbSNP:121434259
613 613 a, g dbSNP:368773485
620 620 c, t dbSNP:757125309
623 623 c, g, t dbSNP:780872661
628 628 c, t dbSNP:121434261
629 629 c, t dbSNP:373178836
633 633 c, t dbSNP:779218238
637 637 a, g dbSNP:541636141
639 639 a, t dbSNP:772274240
644 644 a, g dbSNP:557924117
645 645 a, g dbSNP:778460074
647 647 c, t dbSNP:747447569
648 648 -, aaggaca dbSNP:587776565
651 651 g, t dbSNP:559558673
675 675 a, g dbSNP:17851784
686 686 -, actgga dbSNP:777858863
689 689 a, g dbSNP:371270318
694 694 a, g dbSNP:773740023
695 695 a, c, t dbSNP:374512699
698 698 c, t dbSNP:375802248
699 699 a, g dbSNP:142380707
706 706 a, g dbSNP:547255779
717 717 a, g dbSNP:145935225
722 722 c, g dbSNP:758722464
729 729 -, ttc dbSNP:121434260
739 739 a, g dbSNP:181794923
758 758 c, t dbSNP:751650863
761 761 a, g dbSNP:535626069
768 768 c, t dbSNP:201603384
777 777 a, g dbSNP:781593146
788 788 a, t dbSNP:746369012
790 790 a, g dbSNP:371373672
797 797 c, t dbSNP:756564761
809 809 a, g dbSNP:750431087
811 811 a, g dbSNP:139779327
819 819 a, g dbSNP:780483061
839 839 c, t dbSNP:749708226
841 841 c, g dbSNP:759344163
848 848 a, t dbSNP:778469232
869 869 g, t dbSNP:747813808
879 879 a, g dbSNP:771572024
902 902 c, t dbSNP:142972020
908 908 c, t dbSNP:374911526
921 921 c, t dbSNP:150667239
940 940 a, t dbSNP:779353677
947 947 a, g dbSNP:182990439
958 958 a, g dbSNP:752963731
969 969 c, t dbSNP:756922719
974 974 c, t dbSNP:780880330
981 981 a, g dbSNP:745315897
986 986 a, g dbSNP:139838280
987 987 g, t dbSNP:74315495
1019 1019 c, t dbSNP:149803133
1023 1023 g, t dbSNP:374494685
1030 1030 a, g dbSNP:749176138
1056 1056 a, g dbSNP:141629512
1057 1057 c, t dbSNP:747871414
1094 1094 c, t dbSNP:367802186
1095 1095 a, g dbSNP:776818377
1105 1105 a, g dbSNP:746025177
1117 1117 a, g dbSNP:543087642
1126 1126 a, g dbSNP:145384260
1134 1134 c, g dbSNP:770019352
1142 1142 g, t dbSNP:780003734
1148 1148 a, c dbSNP:749331641
1156 1156 c, t dbSNP:761195572
1163 1163 g, t dbSNP:769348972
1167 1167 a, c dbSNP:771371228
1170 1170 a, g dbSNP:774996651
1181 1181 c, t dbSNP:147665163
1191 1191 c, t dbSNP:772540432
1201 1201 a, g dbSNP:773573049
1202 1202 a, g dbSNP:760272926
1205 1205 a, c dbSNP:765985886
1212 1212 c, t dbSNP:777059212
1213 1213 c, t dbSNP:753300935
1214 1214 a, g dbSNP:759032771
1226 1226 c, t dbSNP:765223726
1227 1227 c, t dbSNP:74315496
1238 1238 a, g dbSNP:376609988
1269 1269 c, t dbSNP:764702893
1270 1270 c, t dbSNP:549225513
1274 1274 g, t dbSNP:762883753
1294 1294 a, g, t dbSNP:764034925
1300 1300 a, g dbSNP:757074151
1304 1304 c, t dbSNP:143160499
1310 1310 a, g dbSNP:766554538
1314 1314 c, t dbSNP:754093587
1315 1315 a, g dbSNP:755200117
1322 1322 c, t dbSNP:151198477
1325 1325 a, g dbSNP:146166875
1334 1334 c, t dbSNP:755253391
1340 1340 a, g dbSNP:569956351
1344 1344 a, g dbSNP:752879150
1346 1346 c, t dbSNP:199957176
1355 1355 a, c, t dbSNP:200272173
1382 1382 c, t dbSNP:747526252
1390 1390 g, t dbSNP:750633919
1391 1391 g, t dbSNP:373606097
1401 1401 c, t dbSNP:74315497
1409 1409 a, g, t dbSNP:746175548
1412 1412 c, t dbSNP:775119805
1413 1413 c, t dbSNP:74315500
1420 1420 a, g dbSNP:748792522
1424 1424 a, g dbSNP:768194921
1426 1426 a, t dbSNP:200372028
1438 1438 -, a dbSNP:587776563
1449 1449 c, t dbSNP:140266312
1450 1450 a, g dbSNP:587778554
1455 1455 c, t dbSNP:761795291
1456 1456 a, g dbSNP:768053145
1457 1457 c, t dbSNP:750911082
1460 1460 c, t dbSNP:756568857
1461 1461 a, g dbSNP:780430071
1464 1464 c, t dbSNP:74315499
1465 1465 a, g, t dbSNP:754087071
1475 1475 c, g dbSNP:2229064
1491 1491 a, g dbSNP:778412102
1494 1494 c, t dbSNP:747756728
1495 1495 a, g dbSNP:771675702
1498 1498 c, t dbSNP:764441073
1499 1499 a, g, t dbSNP:145427713
1517 1517 c, g dbSNP:770949972
1522 1522 c, t dbSNP:74315492
1527 1527 c, t dbSNP:74315498
1530 1530 a, g dbSNP:776499914
1549 1549 c, t dbSNP:745637107
1555 1555 a, g dbSNP:577940601
1556 1556 c, t dbSNP:142459414
1560 1560 a, g dbSNP:761933185
1571 1571 c, g dbSNP:757322524
1582 1582 c, g dbSNP:780987048
1598 1598 c, t dbSNP:745717285
1606 1606 c, t dbSNP:587778553
1616 1616 c, t dbSNP:769454739
1649 1649 c, t dbSNP:137953976
1651 1651 c, t dbSNP:546551743
1655 1655 a, g dbSNP:748403377
1658 1658 g, t dbSNP:772219612
1662 1662 c, t dbSNP:74315501
1674 1674 c, t dbSNP:773296925
1675 1675 a, g dbSNP:201214090
1680 1680 a, g dbSNP:766974263
1686 1686 a, c dbSNP:776970251
1691 1691 a, g dbSNP:759993776
1695 1695 c, t dbSNP:765540111
1696 1696 a, g dbSNP:548217466
1700 1700 c, g dbSNP:375969005
1701 1701 a, g dbSNP:758128105
1713 1713 c, t dbSNP:763826793
1714 1714 a, g, t dbSNP:751182657
1715 1715 a, c dbSNP:781241303
1716 1716 c, t dbSNP:142446775
1723 1723 a, g dbSNP:760382219
1724 1724 a, g dbSNP:113715304
1733 1733 a, g dbSNP:756041768
1741 1741 c, t dbSNP:779721863
1742 1742 c, t dbSNP:749028891
1746 1746 g, t dbSNP:772334382
1754 1754 a, g dbSNP:144628209
1755 1755 c, t dbSNP:747129449
1817 1817 g, t dbSNP:747252414
1827 1827 c, t dbSNP:771143279
1828 1828 a, g dbSNP:373650983
1829 1829 c, t dbSNP:138354622
1830 1830 a, g, t dbSNP:74315503
1834 1834 c, t dbSNP:776109136
1835 1835 a, g dbSNP:375819833
1839 1839 c, t dbSNP:74315504
1859 1859 c, t dbSNP:148776784
1868 1868 a, t dbSNP:774818801
1873 1873 c, t dbSNP:761378464
1878 1878 c, t dbSNP:768587800
1882 1882 c, t dbSNP:145666157
1883 1883 a, g dbSNP:774373946
1886 1886 c, t dbSNP:760224815
1888 1888 c, t dbSNP:766339217
1889 1889 a, g dbSNP:753751373
1891 1891 c, t dbSNP:771692777
1894 1894 c, t dbSNP:141538143
1898 1898 c, t dbSNP:368966931
1902 1902 a, g dbSNP:147506929
1911 1911 c, g, t dbSNP:776076922
1912 1912 c, t dbSNP:765100922
1913 1913 a, g, t dbSNP:373442542
1943 1943 c, t dbSNP:758182356
1945 1945 c, t dbSNP:767682136
1948 1948 a, g dbSNP:552115565
1954 1954 g, t dbSNP:756366940
1961 1961 a, t dbSNP:780287551
1965 1965 a, g dbSNP:749326764
1975 1975 a, t dbSNP:140025001
1983 1983 a, g dbSNP:201527155
1989 1989 c, t dbSNP:762588182
1991 1991 g, t dbSNP:534398531
2022 2022 g, t dbSNP:74315505
2024 2024 a, t dbSNP:770627371
2029 2029 c, t dbSNP:780818183
2041 2041 a, g dbSNP:745432431
2043 2043 c, t dbSNP:769370159
2047 2047 c, t dbSNP:74315493
2056 2056 a, c dbSNP:74315494
2062 2062 a, g dbSNP:774824164
2073 2073 a, g dbSNP:762953921
2076 2076 c, g dbSNP:768422432
2081 2081 c, t dbSNP:774264046
2082 2082 a, g dbSNP:199669486
2110 2110 c, t dbSNP:766752048
2112 2112 a, g dbSNP:754087219
2114 2114 c, t dbSNP:759780271
2115 2115 c, g dbSNP:765386271
2121 2121 a, g, t dbSNP:557347747
2126 2126 a, g dbSNP:758955756
2127 2127 c, t dbSNP:778127814
2131 2131 a, g dbSNP:768303416
2141 2141 c, t dbSNP:751987156
2142 2142 a, g dbSNP:757586383
2145 2145 -, ag dbSNP:755032702
2149 2149 a, g dbSNP:781488145
2181 2181 c, t dbSNP:749475992
2186 2186 c, t dbSNP:768807694
2192 2192 a, g dbSNP:201911915
2194 2194 a, g dbSNP:761874138
2195 2195 c, t dbSNP:370999558
2196 2196 a, g dbSNP:145446060
2203 2203 c, t dbSNP:761191666
2206 2206 a, g dbSNP:766689587
2210 2210 a, g dbSNP:754168138
2214 2214 c, t dbSNP:759278621
2217 2217 c, t dbSNP:764972504
2235 2235 a, g dbSNP:141099051
2238 2238 a, c dbSNP:757952483
2240 2240 a, c dbSNP:777907253
2241 2241 c, g, t dbSNP:751474583
2246 2246 a, c dbSNP:780862097
2254 2254 a, c dbSNP:200740197
2259 2259 c, g dbSNP:768930578
2265 2265 -, ctc dbSNP:776194172
2267 2267 c, t dbSNP:536441436
2269 2269 c, t dbSNP:748272771
2273 2273 a, t dbSNP:772158241
2274 2274 c, g dbSNP:773299250
2275 2275 a, c, t dbSNP:374219104
2276 2276 a, g dbSNP:377393360
2279 2279 a, g dbSNP:759977916
2281 2281 c, t dbSNP:765024618
2282 2282 g, t dbSNP:554849830
2283 2283 a, c, t dbSNP:370016332
2284 2284 a, g dbSNP:751023311
2296 2296 a, g dbSNP:746841804
2301 2301 a, g dbSNP:754084562
2356 2356 c, t dbSNP:537177702
2363 2363 a, c dbSNP:3180505
2391 2391 g, t dbSNP:558494389
2420 2420 a, c dbSNP:754717476
2464 2464 c, t dbSNP:778408052
2488 2488 a, g dbSNP:531470040
2490 2490 c, g dbSNP:545104019
2511 2511 c, t dbSNP:576785860
2567 2567 a, g dbSNP:373511643
2585 2585 c, t dbSNP:1008515
2590 2590 a, g dbSNP:572307337
2619 2619 c, t dbSNP:777219109
2651 2651 c, t dbSNP:541235064
2681 2681 c, g dbSNP:557890822
2707 2707 c, t dbSNP:577882884
2734 2734 c, t dbSNP:191509325
2768 2768 c, t dbSNP:542811466
2792 2792 c, t dbSNP:776610927
2804 2804 c, t dbSNP:543669369
2805 2805 a, g dbSNP:561178202
2809 2809 c, t dbSNP:531658450
2820 2820 c, g dbSNP:770711462
2862 2862 a, g dbSNP:201571578
2876 2876 a, g dbSNP:543529572
2878 2878 a, g dbSNP:781112228
2935 2935 -, a dbSNP:567370520
2947 2947 a, g dbSNP:565234426
2951 2951 a, g dbSNP:184043147
2976 2976 -, c dbSNP:761028781
3024 3024 c, t dbSNP:562778105
3025 3025 a, g dbSNP:374557247
3063 3063 a, g dbSNP:186559222
3107 3107 c, t dbSNP:149840456
3165 3165 c, g dbSNP:145820299
3188 3188 c, t dbSNP:531577838
3205 3205 c, t dbSNP:8140096
3221 3221 a, g dbSNP:569883007
3307 3307 a, c dbSNP:762085797
3318 3318 c, t dbSNP:148973148
3330 3330 c, g dbSNP:773367316
3348 3348 c, t dbSNP:191183183
3371 3371 g, t dbSNP:145719271
3420 3420 c, t dbSNP:534317366
3423 3423 a, g dbSNP:761248952
3439 3439 c, t dbSNP:2530680
3451 3451 c, t dbSNP:572384896
3501 3501 c, t dbSNP:542927614
3548 3548 -, gtggga dbSNP:148703036
3599 3599 g, t dbSNP:754278601
3607 3607 a, g dbSNP:554713355
3636 3636 a, g dbSNP:759790063
3640 3640 c, g dbSNP:576229506
3669 3669 a, g dbSNP:376061969
3679 3679 g, t dbSNP:765548821
3683 3683 c, t dbSNP:752371775
3704 3704 a, c dbSNP:757990941
3762 3762 c, t dbSNP:565224641
3769 3769 c, g dbSNP:766153036
3787 3787 c, t dbSNP:76245756
3846 3846 g, t dbSNP:532686256
3849 3849 c, t dbSNP:184477204
3871 3871 a, c dbSNP:138312881
3883 3883 g, t dbSNP:751068883
3890 3890 a, g dbSNP:529983928
3903 3903 c, t dbSNP:757272448
3921 3921 a, g dbSNP:12106585
4023 4023 a, g dbSNP:17462054
4054 4054 c, t dbSNP:190590507
4105 4105 g, t dbSNP:530734498
4125 4125 a, g dbSNP:141268194
4135 4135 a, g dbSNP:73390944
4177 4177 c, t dbSNP:534745766
4178 4178 a, g dbSNP:546242180
4195 4195 a, g dbSNP:567737516
4249 4249 -, ttctg dbSNP:200967076
4253 4253 -, t, tt dbSNP:11442202
4263 4263 -, t dbSNP:397939715
4280 4280 c, g dbSNP:536498397
4284 4284 c, t dbSNP:193146795
4285 4285 g, t dbSNP:554850216
4308 4308 c, t dbSNP:144993615
4344 4344 c, t dbSNP:537244098
4356 4356 c, g dbSNP:559133039
4371 4371 a, g dbSNP:371637488
4390 4390 c, t dbSNP:550425263
4447 4447 c, t dbSNP:577249709
4452 4452 a, g dbSNP:541531112
4455 4455 a, g dbSNP:112175950
4466 4466 a, g dbSNP:5997506
4476 4476 c, g dbSNP:555359252
4480 4480 a, c dbSNP:773492147
4497 4497 c, t dbSNP:563567208
4498 4498 -, gct dbSNP:756940867
4518 4518 c, t dbSNP:77461085
4521 4521 c, t dbSNP:78535993
4526 4526 a, g dbSNP:771509575
4529 4529 a, t dbSNP:76646957
4541 4541 c, t dbSNP:777037756
4542 4542 a, g dbSNP:113956497
4574 4574 a, g dbSNP:534681348
4584 4584 c, g dbSNP:557684656
4596 4596 c, t dbSNP:765602116
4600 4600 c, t dbSNP:150581809
4639 4639 a, g dbSNP:114416141
4681 4681 c, t dbSNP:528145340
4686 4686 c, t dbSNP:73159090
4694 4694 a, c dbSNP:780221968
4695 4695 a, g dbSNP:185127651
4699 4699 c, g dbSNP:1034880
4718 4718 a, g dbSNP:550207111
4730 4730 c, t dbSNP:569992376
4782 4782 g, t dbSNP:537782319
4838 4838 c, t dbSNP:138606078
4855 4855 a, c dbSNP:768487274
4901 4901 c, t dbSNP:763727745
4908 4908 a, g dbSNP:565795130
4923 4923 a, t dbSNP:780919357
4984 4984 a, g dbSNP:199805259
4990 4990 a, g dbSNP:5763430
4995 4995 -, t dbSNP:771811332
4996 4996 -, t dbSNP:113760386
5011 5011 g, t dbSNP:77381275
5015 5015 a, g dbSNP:769634820
5035 5035 a, g dbSNP:189146105
5072 5072 c, t dbSNP:142797364
5079 5079 c, t dbSNP:575028212
5081 5081 c, t dbSNP:75293087
5091 5091 a, t dbSNP:76134776
5107 5107 c, t dbSNP:5763431
5115 5115 c, t dbSNP:181171324
5120 5120 c, t dbSNP:575435924
5153 5153 a, g dbSNP:112930471
5154 5154 c, t dbSNP:111641882
5156 5156 c, t dbSNP:564077209
5176 5176 g, t dbSNP:780709660
5188 5188 c, t dbSNP:74528592
5201 5201 a, g dbSNP:185938514
5204 5204 a, g dbSNP:190002811
5212 5212 c, t dbSNP:182522654
5277 5277 a, g dbSNP:750347453
5302 5302 a, g dbSNP:145080771
5309 5309 g, t dbSNP:117601743
5314 5314 c, t dbSNP:774123103
5364 5364 a, g dbSNP:527795536
5373 5373 a, g dbSNP:756019592
5397 5397 c, t dbSNP:764512220
5432 5432 c, t dbSNP:185621751
5439 5439 a, g dbSNP:117863523
5505 5505 a, g dbSNP:11090576
5510 5510 c, t dbSNP:369250167
5534 5534 a, g dbSNP:531083925
5544 5544 a, t dbSNP:188812769
5549 5549 -, g dbSNP:372162228
5558 5558 g, t dbSNP:552839202
5572 5572 a, c dbSNP:570917052
5636 5636 c, t dbSNP:533566312
5657 5657 a, t dbSNP:11537543
5698 5698 c, t dbSNP:535211691
5753 5753 -, t dbSNP:749974930
5784 5784 c, t dbSNP:553652217
5805 5805 c, t dbSNP:568548162
5806 5806 a, g dbSNP:41278851
5834 5834 a, c dbSNP:557359614
5841 5841 -, gcccaa dbSNP:755969033
5844 5844 -, a dbSNP:375908855
5875 5875 c, t dbSNP:372862060
5912 5912 c, t dbSNP:537735494
5921 5921 c, t dbSNP:556223075
5933 5933 c, t dbSNP:747215922

Target ORF information:

RefSeq Version NM_000268
Organism Homo sapiens (human)
Definition Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000268

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGATGAAG
TGGAAAGGGA AGGACCTCTT TGATTTGGTG TGCCGGACTC TGGGGCTCCG AGAAACCTGG
TTCTTTGGAC TGCAGTACAC AATCAAGGAC ACAGTGGCCT GGCTCAAAAT GGACAAGAAG
GTACTGGATC ATGATGTTTC AAAGGAAGAA CCAGTCACCT TTCACTTCTT GGCCAAATTT
TATCCTGAGA ATGCTGAAGA GGAGCTGGTT CAGGAGATCA CACAACATTT ATTCTTCTTA
CAGGTAAAGA AGCAGATTTT AGATGAAAAG ATCTACTGCC CTCCTGAGGC TTCTGTGCTC
CTGGCTTCTT ACGCCGTCCA GGCCAAGTAT GGTGACTACG ACCCCAGTGT TCACAAGCGG
GGATTTTTGG CCCAAGAGGA ATTGCTTCCA AAAAGGGTAA TAAATCTGTA TCAGATGACT
CCGGAAATGT GGGAGGAGAG AATTACTGCT TGGTACGCAG AGCACCGAGG CCGAGCCAGG
GATGAAGCTG AAATGGAATA TCTGAAGATA GCTCAGGACC TGGAGATGTA CGGTGTGAAC
TACTTTGCAA TCCGGAATAA AAAGGGCACA GAGCTGCTGC TTGGAGTGGA TGCCCTGGGG
CTTCACATTT ATGACCCTGA GAACAGACTG ACCCCCAAGA TCTCCTTCCC GTGGAATGAA
ATCCGAAACA TCTCGTACAG TGACAAGGAG TTTACTATTA AACCACTGGA TAAGAAAATT
GATGTCTTCA AGTTTAACTC CTCAAAGCTT CGTGTTAATA AGCTGATTCT CCAGCTATGT
ATCGGGAACC ATGATCTATT TATGAGGAGA AGGAAAGCCG ATTCTTTGGA AGTTCAGCAG
ATGAAAGCCC AGGCCAGGGA GGAGAAGGCT AGAAAGCAGA TGGAGCGGCA GCGCCTCGCT
CGAGAGAAGC AGATGAGGGA GGAGGCTGAA CGCACGAGGG ATGAGTTGGA GAGGAGGCTG
CTGCAGATGA AAGAAGAAGC AACAATGGCC AACGAAGCAC TGATGCGGTC TGAGGAGACA
GCTGACCTGT TGGCTGAAAA GGCCCAGATC ACCGAGGAGG AGGCAAAACT TCTGGCCCAG
AAGGCCGCAG AGGCTGAGCA GGAAATGCAG CGCATCAAGG CCACAGCGAT TCGCACGGAG
GAGGAGAAGC GCCTGATGGA GCAGAAGGTG CTGGAAGCCG AGGTGCTGGC ACTGAAGATG
GCTGAGGAGT CAGAGAGGAG GGCCAAAGAG GCAGATCAGC TGAAGCAGGA CCTGCAGGAA
GCACGCGAGG CGGAGCGAAG AGCCAAGCAG AAGCTCCTGG AGATTGCCAC CAAGCCCACG
TACCCGCCCA TGAACCCAAT TCCAGCACCG TTGCCTCCTG ACATACCAAG CTTCAACCTC
ATTGGTGACA GCCTGTCTTT CGACTTCAAA GATACTGACA TGAAGCGGCT TTCCATGGAG
ATAGAGAAAG AAAAAGTGGA ATACATGGAA AAGAGCAAGC ATCTGCAGGA GCAGCTCAAT
GAACTCAAGA CAGAAATCGA GGCCTTGAAA CTGAAAGAGA GGGAGACAGC TCTGGATATT
CTGCACAATG AGAACTCCGA CAGGGGTGGC AGCAGCAAGC ACAATACCAT TAAAAAGCTC
ACCTTGCAGA GCGCCAAGTC CCGAGTGGCC TTCTTTGAAG AGCTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16401
Accession Version NM_016418.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1773bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product merlin isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN419318.1, AF369658.1, AC004882.3 and BC071653.1. This sequence is a reference standard in the RefSeqGene project. On Dec 20, 2007 this sequence version replaced gi:32967274. Summary: This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (2) includes an alternate exon, compared to variant 1, that causes a frameshift. The resulting protein (isoform 2), one of the two predominant isoforms, has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF369658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGATGAAG
TGGAAAGGGA AGGACCTCTT TGATTTGGTG TGCCGGACTC TGGGGCTCCG AGAAACCTGG
TTCTTTGGAC TGCAGTACAC AATCAAGGAC ACAGTGGCCT GGCTCAAAAT GGACAAGAAG
GTACTGGATC ATGATGTTTC AAAGGAAGAA CCAGTCACCT TTCACTTCTT GGCCAAATTT
TATCCTGAGA ATGCTGAAGA GGAGCTGGTT CAGGAGATCA CACAACATTT ATTCTTCTTA
CAGGTAAAGA AGCAGATTTT AGATGAAAAG ATCTACTGCC CTCCTGAGGC TTCTGTGCTC
CTGGCTTCTT ACGCCGTCCA GGCCAAGTAT GGTGACTACG ACCCCAGTGT TCACAAGCGG
GGATTTTTGG CCCAAGAGGA ATTGCTTCCA AAAAGGGTAA TAAATCTGTA TCAGATGACT
CCGGAAATGT GGGAGGAGAG AATTACTGCT TGGTACGCAG AGCACCGAGG CCGAGCCAGG
GATGAAGCTG AAATGGAATA TCTGAAGATA GCTCAGGACC TGGAGATGTA CGGTGTGAAC
TACTTTGCAA TCCGGAATAA AAAGGGCACA GAGCTGCTGC TTGGAGTGGA TGCCCTGGGG
CTTCACATTT ATGACCCTGA GAACAGACTG ACCCCCAAGA TCTCCTTCCC GTGGAATGAA
ATCCGAAACA TCTCGTACAG TGACAAGGAG TTTACTATTA AACCACTGGA TAAGAAAATT
GATGTCTTCA AGTTTAACTC CTCAAAGCTT CGTGTTAATA AGCTGATTCT CCAGCTATGT
ATCGGGAACC ATGATCTATT TATGAGGAGA AGGAAAGCCG ATTCTTTGGA AGTTCAGCAG
ATGAAAGCCC AGGCCAGGGA GGAGAAGGCT AGAAAGCAGA TGGAGCGGCA GCGCCTCGCT
CGAGAGAAGC AGATGAGGGA GGAGGCTGAA CGCACGAGGG ATGAGTTGGA GAGGAGGCTG
CTGCAGATGA AAGAAGAAGC AACAATGGCC AACGAAGCAC TGATGCGGTC TGAGGAGACA
GCTGACCTGT TGGCTGAAAA GGCCCAGATC ACCGAGGAGG AGGCAAAACT TCTGGCCCAG
AAGGCCGCAG AGGCTGAGCA GGAAATGCAG CGCATCAAGG CCACAGCGAT TCGCACGGAG
GAGGAGAAGC GCCTGATGGA GCAGAAGGTG CTGGAAGCCG AGGTGCTGGC ACTGAAGATG
GCTGAGGAGT CAGAGAGGAG GGCCAAAGAG GCAGATCAGC TGAAGCAGGA CCTGCAGGAA
GCACGCGAGG CGGAGCGAAG AGCCAAGCAG AAGCTCCTGG AGATTGCCAC CAAGCCCACG
TACCCGCCCA TGAACCCAAT TCCAGCACCG TTGCCTCCTG ACATACCAAG CTTCAACCTC
ATTGGTGACA GCCTGTCTTT CGACTTCAAA GATACTGACA TGAAGCGGCT TTCCATGGAG
ATAGAGAAAG AAAAAGTGGA ATACATGGAA AAGAGCAAGC ATCTGCAGGA GCAGCTCAAT
GAACTCAAGA CAGAAATCGA GGCCTTGAAA CTGAAAGAGA GGGAGACAGC TCTGGATATT
CTGCACAATG AGAACTCCGA CAGGGGTGGC AGCAGCAAGC ACAATACCAT TAAAAAGCCT
CAAGCCCAAG GCAGAAGACC TATCTGCATT TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_057502.2
CDS444..2216
Misc Feature(1)357..359(+)
Misc Feature(2)510..1109(+)
Misc Feature(3)519..749(+)
Misc Feature(4)783..1085(+)
Misc Feature(5)1089..1379(+)
Misc Feature(6)1215..1355(+)
Misc Feature(7)1215..1355(+)
Misc Feature(8)1323..1337(+)
Misc Feature(9)1332..1379(+)
Misc Feature(10)1404..2066(+)
Misc Feature(11)1482..2180(+)
Misc Feature(12)1995..1997(+)
Misc Feature(13)1995..1997(+)
Misc Feature(14)1995..1997(+)
Exon (1)1..557
Gene:NF2
Gene Synonym:
Exon (2)558..683
Gene:NF2
Gene Synonym:
Exon (3)684..806
Gene:NF2
Gene Synonym:
Exon (4)807..890
Gene:NF2
Gene Synonym:
Exon (5)891..959
Gene:NF2
Gene Synonym:
Exon (6)960..1042
Gene:NF2
Gene Synonym:
Exon (7)1043..1118
Gene:NF2
Gene Synonym:
Exon (8)1119..1253
Gene:NF2
Gene Synonym:
Exon (9)1254..1328
Gene:NF2
Gene Synonym:
Exon (10)1329..1442
Gene:NF2
Gene Synonym:
Exon (11)1443..1565
Gene:NF2
Gene Synonym:
Exon (12)1566..1783
Gene:NF2
Gene Synonym:
Exon (13)1784..1889
Gene:NF2
Gene Synonym:
Exon (14)1890..2017
Gene:NF2
Gene Synonym:
Exon (15)2018..2180
Gene:NF2
Gene Synonym:
Exon (16)2181..2225
Gene:NF2
Gene Synonym:
Exon (17)2226..6074
Gene:NF2
Gene Synonym:
Translation
Position Chain Variation Link
40 40 c, t dbSNP:570135699
55 55 c, t dbSNP:539283977
56 56 a, g dbSNP:777179356
103 103 a, c dbSNP:571842282
114 114 g, t dbSNP:554177413
198 198 c, g dbSNP:56733822
199 199 c, g dbSNP:536438675
208 208 c, t dbSNP:555236806
240 240 a, c dbSNP:1800539
334 334 c, g dbSNP:1800540
341 341 c, g dbSNP:1042230
360 360 c, g dbSNP:558960493
396 396 a, c dbSNP:368022063
398 398 a, g dbSNP:753401671
405 405 c, g dbSNP:3181682
416 416 c, t dbSNP:763602804
425 425 c, t dbSNP:577180052
426 426 a, g dbSNP:201591536
427 427 a, g dbSNP:748043566
430 430 c, t dbSNP:758236169
435 435 c, t dbSNP:777578586
436 436 c, t dbSNP:746741839
442 442 c, g dbSNP:559386105
455 455 c, t dbSNP:144477078
466 466 c, g dbSNP:775564806
467 467 a, c dbSNP:749452887
479 479 c, t dbSNP:371800843
491 491 a, c, g dbSNP:774973059
500 500 c, g dbSNP:541641232
514 514 c, t dbSNP:773714780
515 515 c, g dbSNP:761062232
532 532 a, g dbSNP:563168478
537 537 a, g dbSNP:373337083
543 543 c, g dbSNP:753425376
550 550 a, g dbSNP:372279458
558 558 a, g dbSNP:761188569
568 568 -, g dbSNP:587776564
576 576 g, t dbSNP:370147621
578 578 c, t dbSNP:752664255
588 588 a, t dbSNP:759129920
597 597 c, t dbSNP:764901064
603 603 c, t dbSNP:752220604
604 604 g, t dbSNP:762440698
606 606 a, g dbSNP:764089682
612 612 c, t dbSNP:121434259
613 613 a, g dbSNP:368773485
620 620 c, t dbSNP:757125309
623 623 c, g, t dbSNP:780872661
628 628 c, t dbSNP:121434261
629 629 c, t dbSNP:373178836
633 633 c, t dbSNP:779218238
637 637 a, g dbSNP:541636141
639 639 a, t dbSNP:772274240
644 644 a, g dbSNP:557924117
645 645 a, g dbSNP:778460074
647 647 c, t dbSNP:747447569
648 648 -, aaggaca dbSNP:587776565
651 651 g, t dbSNP:559558673
675 675 a, g dbSNP:17851784
686 686 -, actgga dbSNP:777858863
689 689 a, g dbSNP:371270318
694 694 a, g dbSNP:773740023
695 695 a, c, t dbSNP:374512699
698 698 c, t dbSNP:375802248
699 699 a, g dbSNP:142380707
706 706 a, g dbSNP:547255779
717 717 a, g dbSNP:145935225
722 722 c, g dbSNP:758722464
729 729 -, ttc dbSNP:121434260
739 739 a, g dbSNP:181794923
758 758 c, t dbSNP:751650863
761 761 a, g dbSNP:535626069
768 768 c, t dbSNP:201603384
777 777 a, g dbSNP:781593146
788 788 a, t dbSNP:746369012
790 790 a, g dbSNP:371373672
797 797 c, t dbSNP:756564761
809 809 a, g dbSNP:750431087
811 811 a, g dbSNP:139779327
819 819 a, g dbSNP:780483061
839 839 c, t dbSNP:749708226
841 841 c, g dbSNP:759344163
848 848 a, t dbSNP:778469232
869 869 g, t dbSNP:747813808
879 879 a, g dbSNP:771572024
902 902 c, t dbSNP:142972020
908 908 c, t dbSNP:374911526
921 921 c, t dbSNP:150667239
940 940 a, t dbSNP:779353677
947 947 a, g dbSNP:182990439
958 958 a, g dbSNP:752963731
969 969 c, t dbSNP:756922719
974 974 c, t dbSNP:780880330
981 981 a, g dbSNP:745315897
986 986 a, g dbSNP:139838280
987 987 g, t dbSNP:74315495
1019 1019 c, t dbSNP:149803133
1023 1023 g, t dbSNP:374494685
1030 1030 a, g dbSNP:749176138
1056 1056 a, g dbSNP:141629512
1057 1057 c, t dbSNP:747871414
1094 1094 c, t dbSNP:367802186
1095 1095 a, g dbSNP:776818377
1105 1105 a, g dbSNP:746025177
1117 1117 a, g dbSNP:543087642
1126 1126 a, g dbSNP:145384260
1134 1134 c, g dbSNP:770019352
1142 1142 g, t dbSNP:780003734
1148 1148 a, c dbSNP:749331641
1156 1156 c, t dbSNP:761195572
1163 1163 g, t dbSNP:769348972
1167 1167 a, c dbSNP:771371228
1170 1170 a, g dbSNP:774996651
1181 1181 c, t dbSNP:147665163
1191 1191 c, t dbSNP:772540432
1201 1201 a, g dbSNP:773573049
1202 1202 a, g dbSNP:760272926
1205 1205 a, c dbSNP:765985886
1212 1212 c, t dbSNP:777059212
1213 1213 c, t dbSNP:753300935
1214 1214 a, g dbSNP:759032771
1226 1226 c, t dbSNP:765223726
1227 1227 c, t dbSNP:74315496
1238 1238 a, g dbSNP:376609988
1269 1269 c, t dbSNP:764702893
1270 1270 c, t dbSNP:549225513
1274 1274 g, t dbSNP:762883753
1294 1294 a, g, t dbSNP:764034925
1300 1300 a, g dbSNP:757074151
1304 1304 c, t dbSNP:143160499
1310 1310 a, g dbSNP:766554538
1314 1314 c, t dbSNP:754093587
1315 1315 a, g dbSNP:755200117
1322 1322 c, t dbSNP:151198477
1325 1325 a, g dbSNP:146166875
1334 1334 c, t dbSNP:755253391
1340 1340 a, g dbSNP:569956351
1344 1344 a, g dbSNP:752879150
1346 1346 c, t dbSNP:199957176
1355 1355 a, c, t dbSNP:200272173
1382 1382 c, t dbSNP:747526252
1390 1390 g, t dbSNP:750633919
1391 1391 g, t dbSNP:373606097
1401 1401 c, t dbSNP:74315497
1409 1409 a, g, t dbSNP:746175548
1412 1412 c, t dbSNP:775119805
1413 1413 c, t dbSNP:74315500
1420 1420 a, g dbSNP:748792522
1424 1424 a, g dbSNP:768194921
1426 1426 a, t dbSNP:200372028
1438 1438 -, a dbSNP:587776563
1449 1449 c, t dbSNP:140266312
1450 1450 a, g dbSNP:587778554
1455 1455 c, t dbSNP:761795291
1456 1456 a, g dbSNP:768053145
1457 1457 c, t dbSNP:750911082
1460 1460 c, t dbSNP:756568857
1461 1461 a, g dbSNP:780430071
1464 1464 c, t dbSNP:74315499
1465 1465 a, g, t dbSNP:754087071
1475 1475 c, g dbSNP:2229064
1491 1491 a, g dbSNP:778412102
1494 1494 c, t dbSNP:747756728
1495 1495 a, g dbSNP:771675702
1498 1498 c, t dbSNP:764441073
1499 1499 a, g, t dbSNP:145427713
1517 1517 c, g dbSNP:770949972
1522 1522 c, t dbSNP:74315492
1527 1527 c, t dbSNP:74315498
1530 1530 a, g dbSNP:776499914
1549 1549 c, t dbSNP:745637107
1555 1555 a, g dbSNP:577940601
1556 1556 c, t dbSNP:142459414
1560 1560 a, g dbSNP:761933185
1571 1571 c, g dbSNP:757322524
1582 1582 c, g dbSNP:780987048
1598 1598 c, t dbSNP:745717285
1606 1606 c, t dbSNP:587778553
1616 1616 c, t dbSNP:769454739
1649 1649 c, t dbSNP:137953976
1651 1651 c, t dbSNP:546551743
1655 1655 a, g dbSNP:748403377
1658 1658 g, t dbSNP:772219612
1662 1662 c, t dbSNP:74315501
1674 1674 c, t dbSNP:773296925
1675 1675 a, g dbSNP:201214090
1680 1680 a, g dbSNP:766974263
1686 1686 a, c dbSNP:776970251
1691 1691 a, g dbSNP:759993776
1695 1695 c, t dbSNP:765540111
1696 1696 a, g dbSNP:548217466
1700 1700 c, g dbSNP:375969005
1701 1701 a, g dbSNP:758128105
1713 1713 c, t dbSNP:763826793
1714 1714 a, g, t dbSNP:751182657
1715 1715 a, c dbSNP:781241303
1716 1716 c, t dbSNP:142446775
1723 1723 a, g dbSNP:760382219
1724 1724 a, g dbSNP:113715304
1733 1733 a, g dbSNP:756041768
1741 1741 c, t dbSNP:779721863
1742 1742 c, t dbSNP:749028891
1746 1746 g, t dbSNP:772334382
1754 1754 a, g dbSNP:144628209
1755 1755 c, t dbSNP:747129449
1817 1817 g, t dbSNP:747252414
1827 1827 c, t dbSNP:771143279
1828 1828 a, g dbSNP:373650983
1829 1829 c, t dbSNP:138354622
1830 1830 a, g, t dbSNP:74315503
1834 1834 c, t dbSNP:776109136
1835 1835 a, g dbSNP:375819833
1839 1839 c, t dbSNP:74315504
1859 1859 c, t dbSNP:148776784
1868 1868 a, t dbSNP:774818801
1873 1873 c, t dbSNP:761378464
1878 1878 c, t dbSNP:768587800
1882 1882 c, t dbSNP:145666157
1883 1883 a, g dbSNP:774373946
1886 1886 c, t dbSNP:760224815
1888 1888 c, t dbSNP:766339217
1889 1889 a, g dbSNP:753751373
1891 1891 c, t dbSNP:771692777
1894 1894 c, t dbSNP:141538143
1898 1898 c, t dbSNP:368966931
1902 1902 a, g dbSNP:147506929
1911 1911 c, g, t dbSNP:776076922
1912 1912 c, t dbSNP:765100922
1913 1913 a, g, t dbSNP:373442542
1943 1943 c, t dbSNP:758182356
1945 1945 c, t dbSNP:767682136
1948 1948 a, g dbSNP:552115565
1954 1954 g, t dbSNP:756366940
1961 1961 a, t dbSNP:780287551
1965 1965 a, g dbSNP:749326764
1975 1975 a, t dbSNP:140025001
1983 1983 a, g dbSNP:201527155
1989 1989 c, t dbSNP:762588182
1991 1991 g, t dbSNP:534398531
2022 2022 g, t dbSNP:74315505
2024 2024 a, t dbSNP:770627371
2029 2029 c, t dbSNP:780818183
2041 2041 a, g dbSNP:745432431
2043 2043 c, t dbSNP:769370159
2047 2047 c, t dbSNP:74315493
2056 2056 a, c dbSNP:74315494
2062 2062 a, g dbSNP:774824164
2073 2073 a, g dbSNP:762953921
2076 2076 c, g dbSNP:768422432
2081 2081 c, t dbSNP:774264046
2082 2082 a, g dbSNP:199669486
2110 2110 c, t dbSNP:766752048
2112 2112 a, g dbSNP:754087219
2114 2114 c, t dbSNP:759780271
2115 2115 c, g dbSNP:765386271
2121 2121 a, g, t dbSNP:557347747
2126 2126 a, g dbSNP:758955756
2127 2127 c, t dbSNP:778127814
2131 2131 a, g dbSNP:768303416
2141 2141 c, t dbSNP:751987156
2142 2142 a, g dbSNP:757586383
2145 2145 -, ag dbSNP:755032702
2149 2149 a, g dbSNP:781488145
2194 2194 a, g dbSNP:746764835
2205 2205 a, g dbSNP:187478738
2219 2219 c, t dbSNP:770119441
2226 2226 c, t dbSNP:749475992
2231 2231 c, t dbSNP:768807694
2237 2237 a, g dbSNP:201911915
2239 2239 a, g dbSNP:761874138
2240 2240 c, t dbSNP:370999558
2241 2241 a, g dbSNP:145446060
2248 2248 c, t dbSNP:761191666
2251 2251 a, g dbSNP:766689587
2255 2255 a, g dbSNP:754168138
2259 2259 c, t dbSNP:759278621
2262 2262 c, t dbSNP:764972504
2280 2280 a, g dbSNP:141099051
2283 2283 a, c dbSNP:757952483
2285 2285 a, c dbSNP:777907253
2286 2286 c, g, t dbSNP:751474583
2291 2291 a, c dbSNP:780862097
2299 2299 a, c dbSNP:200740197
2304 2304 c, g dbSNP:768930578
2310 2310 -, ctc dbSNP:776194172
2312 2312 c, t dbSNP:536441436
2314 2314 c, t dbSNP:748272771
2318 2318 a, t dbSNP:772158241
2319 2319 c, g dbSNP:773299250
2320 2320 a, c, t dbSNP:374219104
2321 2321 a, g dbSNP:377393360
2324 2324 a, g dbSNP:759977916
2326 2326 c, t dbSNP:765024618
2327 2327 g, t dbSNP:554849830
2328 2328 a, c, t dbSNP:370016332
2329 2329 a, g dbSNP:751023311
2341 2341 a, g dbSNP:746841804
2346 2346 a, g dbSNP:754084562
2401 2401 c, t dbSNP:537177702
2408 2408 a, c dbSNP:3180505
2436 2436 g, t dbSNP:558494389
2465 2465 a, c dbSNP:754717476
2509 2509 c, t dbSNP:778408052
2533 2533 a, g dbSNP:531470040
2535 2535 c, g dbSNP:545104019
2556 2556 c, t dbSNP:576785860
2612 2612 a, g dbSNP:373511643
2630 2630 c, t dbSNP:1008515
2635 2635 a, g dbSNP:572307337
2664 2664 c, t dbSNP:777219109
2696 2696 c, t dbSNP:541235064
2726 2726 c, g dbSNP:557890822
2752 2752 c, t dbSNP:577882884
2779 2779 c, t dbSNP:191509325
2813 2813 c, t dbSNP:542811466
2837 2837 c, t dbSNP:776610927
2849 2849 c, t dbSNP:543669369
2850 2850 a, g dbSNP:561178202
2854 2854 c, t dbSNP:531658450
2865 2865 c, g dbSNP:770711462
2907 2907 a, g dbSNP:201571578
2921 2921 a, g dbSNP:543529572
2923 2923 a, g dbSNP:781112228
2980 2980 -, a dbSNP:567370520
2992 2992 a, g dbSNP:565234426
2996 2996 a, g dbSNP:184043147
3021 3021 -, c dbSNP:761028781
3069 3069 c, t dbSNP:562778105
3070 3070 a, g dbSNP:374557247
3108 3108 a, g dbSNP:186559222
3152 3152 c, t dbSNP:149840456
3210 3210 c, g dbSNP:145820299
3233 3233 c, t dbSNP:531577838
3250 3250 c, t dbSNP:8140096
3266 3266 a, g dbSNP:569883007
3352 3352 a, c dbSNP:762085797
3363 3363 c, t dbSNP:148973148
3375 3375 c, g dbSNP:773367316
3393 3393 c, t dbSNP:191183183
3416 3416 g, t dbSNP:145719271
3465 3465 c, t dbSNP:534317366
3468 3468 a, g dbSNP:761248952
3484 3484 c, t dbSNP:2530680
3496 3496 c, t dbSNP:572384896
3546 3546 c, t dbSNP:542927614
3593 3593 -, gtggga dbSNP:148703036
3644 3644 g, t dbSNP:754278601
3652 3652 a, g dbSNP:554713355
3681 3681 a, g dbSNP:759790063
3685 3685 c, g dbSNP:576229506
3714 3714 a, g dbSNP:376061969
3724 3724 g, t dbSNP:765548821
3728 3728 c, t dbSNP:752371775
3749 3749 a, c dbSNP:757990941
3807 3807 c, t dbSNP:565224641
3814 3814 c, g dbSNP:766153036
3832 3832 c, t dbSNP:76245756
3891 3891 g, t dbSNP:532686256
3894 3894 c, t dbSNP:184477204
3916 3916 a, c dbSNP:138312881
3928 3928 g, t dbSNP:751068883
3935 3935 a, g dbSNP:529983928
3948 3948 c, t dbSNP:757272448
3966 3966 a, g dbSNP:12106585
4068 4068 a, g dbSNP:17462054
4099 4099 c, t dbSNP:190590507
4150 4150 g, t dbSNP:530734498
4170 4170 a, g dbSNP:141268194
4180 4180 a, g dbSNP:73390944
4222 4222 c, t dbSNP:534745766
4223 4223 a, g dbSNP:546242180
4240 4240 a, g dbSNP:567737516
4294 4294 -, ttctg dbSNP:200967076
4298 4298 -, t, tt dbSNP:11442202
4308 4308 -, t dbSNP:397939715
4325 4325 c, g dbSNP:536498397
4329 4329 c, t dbSNP:193146795
4330 4330 g, t dbSNP:554850216
4353 4353 c, t dbSNP:144993615
4389 4389 c, t dbSNP:537244098
4401 4401 c, g dbSNP:559133039
4416 4416 a, g dbSNP:371637488
4435 4435 c, t dbSNP:550425263
4492 4492 c, t dbSNP:577249709
4497 4497 a, g dbSNP:541531112
4500 4500 a, g dbSNP:112175950
4511 4511 a, g dbSNP:5997506
4521 4521 c, g dbSNP:555359252
4525 4525 a, c dbSNP:773492147
4542 4542 c, t dbSNP:563567208
4543 4543 -, gct dbSNP:756940867
4563 4563 c, t dbSNP:77461085
4566 4566 c, t dbSNP:78535993
4571 4571 a, g dbSNP:771509575
4574 4574 a, t dbSNP:76646957
4586 4586 c, t dbSNP:777037756
4587 4587 a, g dbSNP:113956497
4619 4619 a, g dbSNP:534681348
4629 4629 c, g dbSNP:557684656
4641 4641 c, t dbSNP:765602116
4645 4645 c, t dbSNP:150581809
4684 4684 a, g dbSNP:114416141
4726 4726 c, t dbSNP:528145340
4731 4731 c, t dbSNP:73159090
4739 4739 a, c dbSNP:780221968
4740 4740 a, g dbSNP:185127651
4744 4744 c, g dbSNP:1034880
4763 4763 a, g dbSNP:550207111
4775 4775 c, t dbSNP:569992376
4827 4827 g, t dbSNP:537782319
4883 4883 c, t dbSNP:138606078
4900 4900 a, c dbSNP:768487274
4946 4946 c, t dbSNP:763727745
4953 4953 a, g dbSNP:565795130
4968 4968 a, t dbSNP:780919357
5029 5029 a, g dbSNP:199805259
5035 5035 a, g dbSNP:5763430
5040 5040 -, t dbSNP:771811332
5041 5041 -, t dbSNP:113760386
5056 5056 g, t dbSNP:77381275
5060 5060 a, g dbSNP:769634820
5080 5080 a, g dbSNP:189146105
5117 5117 c, t dbSNP:142797364
5124 5124 c, t dbSNP:575028212
5126 5126 c, t dbSNP:75293087
5136 5136 a, t dbSNP:76134776
5152 5152 c, t dbSNP:5763431
5160 5160 c, t dbSNP:181171324
5165 5165 c, t dbSNP:575435924
5198 5198 a, g dbSNP:112930471
5199 5199 c, t dbSNP:111641882
5201 5201 c, t dbSNP:564077209
5221 5221 g, t dbSNP:780709660
5233 5233 c, t dbSNP:74528592
5246 5246 a, g dbSNP:185938514
5249 5249 a, g dbSNP:190002811
5257 5257 c, t dbSNP:182522654
5322 5322 a, g dbSNP:750347453
5347 5347 a, g dbSNP:145080771
5354 5354 g, t dbSNP:117601743
5359 5359 c, t dbSNP:774123103
5409 5409 a, g dbSNP:527795536
5418 5418 a, g dbSNP:756019592
5442 5442 c, t dbSNP:764512220
5477 5477 c, t dbSNP:185621751
5484 5484 a, g dbSNP:117863523
5550 5550 a, g dbSNP:11090576
5555 5555 c, t dbSNP:369250167
5579 5579 a, g dbSNP:531083925
5589 5589 a, t dbSNP:188812769
5594 5594 -, g dbSNP:372162228
5603 5603 g, t dbSNP:552839202
5617 5617 a, c dbSNP:570917052
5681 5681 c, t dbSNP:533566312
5702 5702 a, t dbSNP:11537543
5743 5743 c, t dbSNP:535211691
5798 5798 -, t dbSNP:749974930
5829 5829 c, t dbSNP:553652217
5850 5850 c, t dbSNP:568548162
5851 5851 a, g dbSNP:41278851
5879 5879 a, c dbSNP:557359614
5886 5886 -, gcccaa dbSNP:755969033
5889 5889 -, a dbSNP:375908855
5920 5920 c, t dbSNP:372862060
5957 5957 c, t dbSNP:537735494
5966 5966 c, t dbSNP:556223075
5978 5978 c, t dbSNP:747215922

Target ORF information:

RefSeq Version NM_016418
Organism Homo sapiens (human)
Definition Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_016418

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGATGAAG
TGGAAAGGGA AGGACCTCTT TGATTTGGTG TGCCGGACTC TGGGGCTCCG AGAAACCTGG
TTCTTTGGAC TGCAGTACAC AATCAAGGAC ACAGTGGCCT GGCTCAAAAT GGACAAGAAG
GTACTGGATC ATGATGTTTC AAAGGAAGAA CCAGTCACCT TTCACTTCTT GGCCAAATTT
TATCCTGAGA ATGCTGAAGA GGAGCTGGTT CAGGAGATCA CACAACATTT ATTCTTCTTA
CAGGTAAAGA AGCAGATTTT AGATGAAAAG ATCTACTGCC CTCCTGAGGC TTCTGTGCTC
CTGGCTTCTT ACGCCGTCCA GGCCAAGTAT GGTGACTACG ACCCCAGTGT TCACAAGCGG
GGATTTTTGG CCCAAGAGGA ATTGCTTCCA AAAAGGGTAA TAAATCTGTA TCAGATGACT
CCGGAAATGT GGGAGGAGAG AATTACTGCT TGGTACGCAG AGCACCGAGG CCGAGCCAGG
GATGAAGCTG AAATGGAATA TCTGAAGATA GCTCAGGACC TGGAGATGTA CGGTGTGAAC
TACTTTGCAA TCCGGAATAA AAAGGGCACA GAGCTGCTGC TTGGAGTGGA TGCCCTGGGG
CTTCACATTT ATGACCCTGA GAACAGACTG ACCCCCAAGA TCTCCTTCCC GTGGAATGAA
ATCCGAAACA TCTCGTACAG TGACAAGGAG TTTACTATTA AACCACTGGA TAAGAAAATT
GATGTCTTCA AGTTTAACTC CTCAAAGCTT CGTGTTAATA AGCTGATTCT CCAGCTATGT
ATCGGGAACC ATGATCTATT TATGAGGAGA AGGAAAGCCG ATTCTTTGGA AGTTCAGCAG
ATGAAAGCCC AGGCCAGGGA GGAGAAGGCT AGAAAGCAGA TGGAGCGGCA GCGCCTCGCT
CGAGAGAAGC AGATGAGGGA GGAGGCTGAA CGCACGAGGG ATGAGTTGGA GAGGAGGCTG
CTGCAGATGA AAGAAGAAGC AACAATGGCC AACGAAGCAC TGATGCGGTC TGAGGAGACA
GCTGACCTGT TGGCTGAAAA GGCCCAGATC ACCGAGGAGG AGGCAAAACT TCTGGCCCAG
AAGGCCGCAG AGGCTGAGCA GGAAATGCAG CGCATCAAGG CCACAGCGAT TCGCACGGAG
GAGGAGAAGC GCCTGATGGA GCAGAAGGTG CTGGAAGCCG AGGTGCTGGC ACTGAAGATG
GCTGAGGAGT CAGAGAGGAG GGCCAAAGAG GCAGATCAGC TGAAGCAGGA CCTGCAGGAA
GCACGCGAGG CGGAGCGAAG AGCCAAGCAG AAGCTCCTGG AGATTGCCAC CAAGCCCACG
TACCCGCCCA TGAACCCAAT TCCAGCACCG TTGCCTCCTG ACATACCAAG CTTCAACCTC
ATTGGTGACA GCCTGTCTTT CGACTTCAAA GATACTGACA TGAAGCGGCT TTCCATGGAG
ATAGAGAAAG AAAAAGTGGA ATACATGGAA AAGAGCAAGC ATCTGCAGGA GCAGCTCAAT
GAACTCAAGA CAGAAATCGA GGCCTTGAAA CTGAAAGAGA GGGAGACAGC TCTGGATATT
CTGCACAATG AGAACTCCGA CAGGGGTGGC AGCAGCAAGC ACAATACCAT TAAAAAGCCT
CAAGCCCAAG GCAGAAGACC TATCTGCATT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16463
Accession Version NM_181828.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1647bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product merlin isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN419318.1, AF369658.1, AF369663.1, AC004882.3 and BC071653.1. On Dec 20, 2007 this sequence version replaced gi:32967259. Summary: This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (5) lacks an alternate in-frame exon in the 5' coding region and includes an alternate exon in the 3' coding region, compared to variant 1. The resulting protein (isoform 5, also referred to as isoform delE2) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF369663.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGGTACTG
GATCATGATG TTTCAAAGGA AGAACCAGTC ACCTTTCACT TCTTGGCCAA ATTTTATCCT
GAGAATGCTG AAGAGGAGCT GGTTCAGGAG ATCACACAAC ATTTATTCTT CTTACAGGTA
AAGAAGCAGA TTTTAGATGA AAAGATCTAC TGCCCTCCTG AGGCTTCTGT GCTCCTGGCT
TCTTACGCCG TCCAGGCCAA GTATGGTGAC TACGACCCCA GTGTTCACAA GCGGGGATTT
TTGGCCCAAG AGGAATTGCT TCCAAAAAGG GTAATAAATC TGTATCAGAT GACTCCGGAA
ATGTGGGAGG AGAGAATTAC TGCTTGGTAC GCAGAGCACC GAGGCCGAGC CAGGGATGAA
GCTGAAATGG AATATCTGAA GATAGCTCAG GACCTGGAGA TGTACGGTGT GAACTACTTT
GCAATCCGGA ATAAAAAGGG CACAGAGCTG CTGCTTGGAG TGGATGCCCT GGGGCTTCAC
ATTTATGACC CTGAGAACAG ACTGACCCCC AAGATCTCCT TCCCGTGGAA TGAAATCCGA
AACATCTCGT ACAGTGACAA GGAGTTTACT ATTAAACCAC TGGATAAGAA AATTGATGTC
TTCAAGTTTA ACTCCTCAAA GCTTCGTGTT AATAAGCTGA TTCTCCAGCT ATGTATCGGG
AACCATGATC TATTTATGAG GAGAAGGAAA GCCGATTCTT TGGAAGTTCA GCAGATGAAA
GCCCAGGCCA GGGAGGAGAA GGCTAGAAAG CAGATGGAGC GGCAGCGCCT CGCTCGAGAG
AAGCAGATGA GGGAGGAGGC TGAACGCACG AGGGATGAGT TGGAGAGGAG GCTGCTGCAG
ATGAAAGAAG AAGCAACAAT GGCCAACGAA GCACTGATGC GGTCTGAGGA GACAGCTGAC
CTGTTGGCTG AAAAGGCCCA GATCACCGAG GAGGAGGCAA AACTTCTGGC CCAGAAGGCC
GCAGAGGCTG AGCAGGAAAT GCAGCGCATC AAGGCCACAG CGATTCGCAC GGAGGAGGAG
AAGCGCCTGA TGGAGCAGAA GGTGCTGGAA GCCGAGGTGC TGGCACTGAA GATGGCTGAG
GAGTCAGAGA GGAGGGCCAA AGAGGCAGAT CAGCTGAAGC AGGACCTGCA GGAAGCACGC
GAGGCGGAGC GAAGAGCCAA GCAGAAGCTC CTGGAGATTG CCACCAAGCC CACGTACCCG
CCCATGAACC CAATTCCAGC ACCGTTGCCT CCTGACATAC CAAGCTTCAA CCTCATTGGT
GACAGCCTGT CTTTCGACTT CAAAGATACT GACATGAAGC GGCTTTCCAT GGAGATAGAG
AAAGAAAAAG TGGAATACAT GGAAAAGAGC AAGCATCTGC AGGAGCAGCT CAATGAACTC
AAGACAGAAA TCGAGGCCTT GAAACTGAAA GAGAGGGAGA CAGCTCTGGA TATTCTGCAC
AATGAGAACT CCGACAGGGG TGGCAGCAGC AAGCACAATA CCATTAAAAA GCCTCAAGCC
CAAGGCAGAA GACCTATCTG CATTTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_861966.1
CDS444..2090
Misc Feature(1)357..359(+)
Misc Feature(2)555..983(+)
Misc Feature(3)657..959(+)
Misc Feature(4)963..1253(+)
Misc Feature(5)1089..1229(+)
Misc Feature(6)1089..1229(+)
Misc Feature(7)1197..1211(+)
Misc Feature(8)1206..1253(+)
Misc Feature(9)1278..1940(+)
Misc Feature(10)1356..2054(+)
Misc Feature(11)1869..1871(+)
Misc Feature(12)1869..1871(+)
Exon (1)1..557
Gene:NF2
Gene Synonym:
Exon (2)558..680
Gene:NF2
Gene Synonym:
Exon (3)681..764
Gene:NF2
Gene Synonym:
Exon (4)765..833
Gene:NF2
Gene Synonym:
Exon (5)834..916
Gene:NF2
Gene Synonym:
Exon (6)917..992
Gene:NF2
Gene Synonym:
Exon (7)993..1127
Gene:NF2
Gene Synonym:
Exon (8)1128..1202
Gene:NF2
Gene Synonym:
Exon (9)1203..1316
Gene:NF2
Gene Synonym:
Exon (10)1317..1439
Gene:NF2
Gene Synonym:
Exon (11)1440..1657
Gene:NF2
Gene Synonym:
Exon (12)1658..1763
Gene:NF2
Gene Synonym:
Exon (13)1764..1891
Gene:NF2
Gene Synonym:
Exon (14)1892..2054
Gene:NF2
Gene Synonym:
Exon (15)2055..2099
Gene:NF2
Gene Synonym:
Exon (16)2100..5948
Gene:NF2
Gene Synonym:
Translation
Position Chain Variation Link
40 40 c, t dbSNP:570135699
55 55 c, t dbSNP:539283977
56 56 a, g dbSNP:777179356
103 103 a, c dbSNP:571842282
114 114 g, t dbSNP:554177413
198 198 c, g dbSNP:56733822
199 199 c, g dbSNP:536438675
208 208 c, t dbSNP:555236806
240 240 a, c dbSNP:1800539
334 334 c, g dbSNP:1800540
341 341 c, g dbSNP:1042230
360 360 c, g dbSNP:558960493
396 396 a, c dbSNP:368022063
398 398 a, g dbSNP:753401671
405 405 c, g dbSNP:3181682
416 416 c, t dbSNP:763602804
425 425 c, t dbSNP:577180052
426 426 a, g dbSNP:201591536
427 427 a, g dbSNP:748043566
430 430 c, t dbSNP:758236169
435 435 c, t dbSNP:777578586
436 436 c, t dbSNP:746741839
442 442 c, g dbSNP:559386105
455 455 c, t dbSNP:144477078
466 466 c, g dbSNP:775564806
467 467 a, c dbSNP:749452887
479 479 c, t dbSNP:371800843
491 491 a, c, g dbSNP:774973059
500 500 c, g dbSNP:541641232
514 514 c, t dbSNP:773714780
515 515 c, g dbSNP:761062232
532 532 a, g dbSNP:563168478
537 537 a, g dbSNP:373337083
543 543 c, g dbSNP:753425376
550 550 a, g dbSNP:372279458
560 560 -, actgga dbSNP:777858863
563 563 a, g dbSNP:371270318
568 568 a, g dbSNP:773740023
569 569 a, c, t dbSNP:374512699
572 572 c, t dbSNP:375802248
573 573 a, g dbSNP:142380707
580 580 a, g dbSNP:547255779
591 591 a, g dbSNP:145935225
596 596 c, g dbSNP:758722464
603 603 -, ttc dbSNP:121434260
613 613 a, g dbSNP:181794923
632 632 c, t dbSNP:751650863
635 635 a, g dbSNP:535626069
642 642 c, t dbSNP:201603384
651 651 a, g dbSNP:781593146
662 662 a, t dbSNP:746369012
664 664 a, g dbSNP:371373672
671 671 c, t dbSNP:756564761
683 683 a, g dbSNP:750431087
685 685 a, g dbSNP:139779327
693 693 a, g dbSNP:780483061
713 713 c, t dbSNP:749708226
715 715 c, g dbSNP:759344163
722 722 a, t dbSNP:778469232
743 743 g, t dbSNP:747813808
753 753 a, g dbSNP:771572024
776 776 c, t dbSNP:142972020
782 782 c, t dbSNP:374911526
795 795 c, t dbSNP:150667239
814 814 a, t dbSNP:779353677
821 821 a, g dbSNP:182990439
832 832 a, g dbSNP:752963731
843 843 c, t dbSNP:756922719
848 848 c, t dbSNP:780880330
855 855 a, g dbSNP:745315897
860 860 a, g dbSNP:139838280
861 861 g, t dbSNP:74315495
893 893 c, t dbSNP:149803133
897 897 g, t dbSNP:374494685
904 904 a, g dbSNP:749176138
930 930 a, g dbSNP:141629512
931 931 c, t dbSNP:747871414
968 968 c, t dbSNP:367802186
969 969 a, g dbSNP:776818377
979 979 a, g dbSNP:746025177
991 991 a, g dbSNP:543087642
1000 1000 a, g dbSNP:145384260
1008 1008 c, g dbSNP:770019352
1016 1016 g, t dbSNP:780003734
1022 1022 a, c dbSNP:749331641
1030 1030 c, t dbSNP:761195572
1037 1037 g, t dbSNP:769348972
1041 1041 a, c dbSNP:771371228
1044 1044 a, g dbSNP:774996651
1055 1055 c, t dbSNP:147665163
1065 1065 c, t dbSNP:772540432
1075 1075 a, g dbSNP:773573049
1076 1076 a, g dbSNP:760272926
1079 1079 a, c dbSNP:765985886
1086 1086 c, t dbSNP:777059212
1087 1087 c, t dbSNP:753300935
1088 1088 a, g dbSNP:759032771
1100 1100 c, t dbSNP:765223726
1101 1101 c, t dbSNP:74315496
1112 1112 a, g dbSNP:376609988
1143 1143 c, t dbSNP:764702893
1144 1144 c, t dbSNP:549225513
1148 1148 g, t dbSNP:762883753
1168 1168 a, g, t dbSNP:764034925
1174 1174 a, g dbSNP:757074151
1178 1178 c, t dbSNP:143160499
1184 1184 a, g dbSNP:766554538
1188 1188 c, t dbSNP:754093587
1189 1189 a, g dbSNP:755200117
1196 1196 c, t dbSNP:151198477
1199 1199 a, g dbSNP:146166875
1208 1208 c, t dbSNP:755253391
1214 1214 a, g dbSNP:569956351
1218 1218 a, g dbSNP:752879150
1220 1220 c, t dbSNP:199957176
1229 1229 a, c, t dbSNP:200272173
1256 1256 c, t dbSNP:747526252
1264 1264 g, t dbSNP:750633919
1265 1265 g, t dbSNP:373606097
1275 1275 c, t dbSNP:74315497
1283 1283 a, g, t dbSNP:746175548
1286 1286 c, t dbSNP:775119805
1287 1287 c, t dbSNP:74315500
1294 1294 a, g dbSNP:748792522
1298 1298 a, g dbSNP:768194921
1300 1300 a, t dbSNP:200372028
1312 1312 -, a dbSNP:587776563
1323 1323 c, t dbSNP:140266312
1324 1324 a, g dbSNP:587778554
1329 1329 c, t dbSNP:761795291
1330 1330 a, g dbSNP:768053145
1331 1331 c, t dbSNP:750911082
1334 1334 c, t dbSNP:756568857
1335 1335 a, g dbSNP:780430071
1338 1338 c, t dbSNP:74315499
1339 1339 a, g, t dbSNP:754087071
1349 1349 c, g dbSNP:2229064
1365 1365 a, g dbSNP:778412102
1368 1368 c, t dbSNP:747756728
1369 1369 a, g dbSNP:771675702
1372 1372 c, t dbSNP:764441073
1373 1373 a, g, t dbSNP:145427713
1391 1391 c, g dbSNP:770949972
1396 1396 c, t dbSNP:74315492
1401 1401 c, t dbSNP:74315498
1404 1404 a, g dbSNP:776499914
1423 1423 c, t dbSNP:745637107
1429 1429 a, g dbSNP:577940601
1430 1430 c, t dbSNP:142459414
1434 1434 a, g dbSNP:761933185
1445 1445 c, g dbSNP:757322524
1456 1456 c, g dbSNP:780987048
1472 1472 c, t dbSNP:745717285
1480 1480 c, t dbSNP:587778553
1490 1490 c, t dbSNP:769454739
1523 1523 c, t dbSNP:137953976
1525 1525 c, t dbSNP:546551743
1529 1529 a, g dbSNP:748403377
1532 1532 g, t dbSNP:772219612
1536 1536 c, t dbSNP:74315501
1548 1548 c, t dbSNP:773296925
1549 1549 a, g dbSNP:201214090
1554 1554 a, g dbSNP:766974263
1560 1560 a, c dbSNP:776970251
1565 1565 a, g dbSNP:759993776
1569 1569 c, t dbSNP:765540111
1570 1570 a, g dbSNP:548217466
1574 1574 c, g dbSNP:375969005
1575 1575 a, g dbSNP:758128105
1587 1587 c, t dbSNP:763826793
1588 1588 a, g, t dbSNP:751182657
1589 1589 a, c dbSNP:781241303
1590 1590 c, t dbSNP:142446775
1597 1597 a, g dbSNP:760382219
1598 1598 a, g dbSNP:113715304
1607 1607 a, g dbSNP:756041768
1615 1615 c, t dbSNP:779721863
1616 1616 c, t dbSNP:749028891
1620 1620 g, t dbSNP:772334382
1628 1628 a, g dbSNP:144628209
1629 1629 c, t dbSNP:747129449
1691 1691 g, t dbSNP:747252414
1701 1701 c, t dbSNP:771143279
1702 1702 a, g dbSNP:373650983
1703 1703 c, t dbSNP:138354622
1704 1704 a, g, t dbSNP:74315503
1708 1708 c, t dbSNP:776109136
1709 1709 a, g dbSNP:375819833
1713 1713 c, t dbSNP:74315504
1733 1733 c, t dbSNP:148776784
1742 1742 a, t dbSNP:774818801
1747 1747 c, t dbSNP:761378464
1752 1752 c, t dbSNP:768587800
1756 1756 c, t dbSNP:145666157
1757 1757 a, g dbSNP:774373946
1760 1760 c, t dbSNP:760224815
1762 1762 c, t dbSNP:766339217
1763 1763 a, g dbSNP:753751373
1765 1765 c, t dbSNP:771692777
1768 1768 c, t dbSNP:141538143
1772 1772 c, t dbSNP:368966931
1776 1776 a, g dbSNP:147506929
1785 1785 c, g, t dbSNP:776076922
1786 1786 c, t dbSNP:765100922
1787 1787 a, g, t dbSNP:373442542
1817 1817 c, t dbSNP:758182356
1819 1819 c, t dbSNP:767682136
1822 1822 a, g dbSNP:552115565
1828 1828 g, t dbSNP:756366940
1835 1835 a, t dbSNP:780287551
1839 1839 a, g dbSNP:749326764
1849 1849 a, t dbSNP:140025001
1857 1857 a, g dbSNP:201527155
1863 1863 c, t dbSNP:762588182
1865 1865 g, t dbSNP:534398531
1896 1896 g, t dbSNP:74315505
1898 1898 a, t dbSNP:770627371
1903 1903 c, t dbSNP:780818183
1915 1915 a, g dbSNP:745432431
1917 1917 c, t dbSNP:769370159
1921 1921 c, t dbSNP:74315493
1930 1930 a, c dbSNP:74315494
1936 1936 a, g dbSNP:774824164
1947 1947 a, g dbSNP:762953921
1950 1950 c, g dbSNP:768422432
1955 1955 c, t dbSNP:774264046
1956 1956 a, g dbSNP:199669486
1984 1984 c, t dbSNP:766752048
1986 1986 a, g dbSNP:754087219
1988 1988 c, t dbSNP:759780271
1989 1989 c, g dbSNP:765386271
1995 1995 a, g, t dbSNP:557347747
2000 2000 a, g dbSNP:758955756
2001 2001 c, t dbSNP:778127814
2005 2005 a, g dbSNP:768303416
2015 2015 c, t dbSNP:751987156
2016 2016 a, g dbSNP:757586383
2019 2019 -, ag dbSNP:755032702
2023 2023 a, g dbSNP:781488145
2068 2068 a, g dbSNP:746764835
2079 2079 a, g dbSNP:187478738
2093 2093 c, t dbSNP:770119441
2100 2100 c, t dbSNP:749475992
2105 2105 c, t dbSNP:768807694
2111 2111 a, g dbSNP:201911915
2113 2113 a, g dbSNP:761874138
2114 2114 c, t dbSNP:370999558
2115 2115 a, g dbSNP:145446060
2122 2122 c, t dbSNP:761191666
2125 2125 a, g dbSNP:766689587
2129 2129 a, g dbSNP:754168138
2133 2133 c, t dbSNP:759278621
2136 2136 c, t dbSNP:764972504
2154 2154 a, g dbSNP:141099051
2157 2157 a, c dbSNP:757952483
2159 2159 a, c dbSNP:777907253
2160 2160 c, g, t dbSNP:751474583
2165 2165 a, c dbSNP:780862097
2173 2173 a, c dbSNP:200740197
2178 2178 c, g dbSNP:768930578
2184 2184 -, ctc dbSNP:776194172
2186 2186 c, t dbSNP:536441436
2188 2188 c, t dbSNP:748272771
2192 2192 a, t dbSNP:772158241
2193 2193 c, g dbSNP:773299250
2194 2194 a, c, t dbSNP:374219104
2195 2195 a, g dbSNP:377393360
2198 2198 a, g dbSNP:759977916
2200 2200 c, t dbSNP:765024618
2201 2201 g, t dbSNP:554849830
2202 2202 a, c, t dbSNP:370016332
2203 2203 a, g dbSNP:751023311
2215 2215 a, g dbSNP:746841804
2220 2220 a, g dbSNP:754084562
2275 2275 c, t dbSNP:537177702
2282 2282 a, c dbSNP:3180505
2310 2310 g, t dbSNP:558494389
2339 2339 a, c dbSNP:754717476
2383 2383 c, t dbSNP:778408052
2407 2407 a, g dbSNP:531470040
2409 2409 c, g dbSNP:545104019
2430 2430 c, t dbSNP:576785860
2486 2486 a, g dbSNP:373511643
2504 2504 c, t dbSNP:1008515
2509 2509 a, g dbSNP:572307337
2538 2538 c, t dbSNP:777219109
2570 2570 c, t dbSNP:541235064
2600 2600 c, g dbSNP:557890822
2626 2626 c, t dbSNP:577882884
2653 2653 c, t dbSNP:191509325
2687 2687 c, t dbSNP:542811466
2711 2711 c, t dbSNP:776610927
2723 2723 c, t dbSNP:543669369
2724 2724 a, g dbSNP:561178202
2728 2728 c, t dbSNP:531658450
2739 2739 c, g dbSNP:770711462
2781 2781 a, g dbSNP:201571578
2795 2795 a, g dbSNP:543529572
2797 2797 a, g dbSNP:781112228
2854 2854 -, a dbSNP:567370520
2866 2866 a, g dbSNP:565234426
2870 2870 a, g dbSNP:184043147
2895 2895 -, c dbSNP:761028781
2943 2943 c, t dbSNP:562778105
2944 2944 a, g dbSNP:374557247
2982 2982 a, g dbSNP:186559222
3026 3026 c, t dbSNP:149840456
3084 3084 c, g dbSNP:145820299
3107 3107 c, t dbSNP:531577838
3124 3124 c, t dbSNP:8140096
3140 3140 a, g dbSNP:569883007
3226 3226 a, c dbSNP:762085797
3237 3237 c, t dbSNP:148973148
3249 3249 c, g dbSNP:773367316
3267 3267 c, t dbSNP:191183183
3290 3290 g, t dbSNP:145719271
3339 3339 c, t dbSNP:534317366
3342 3342 a, g dbSNP:761248952
3358 3358 c, t dbSNP:2530680
3370 3370 c, t dbSNP:572384896
3420 3420 c, t dbSNP:542927614
3467 3467 -, gtggga dbSNP:148703036
3518 3518 g, t dbSNP:754278601
3526 3526 a, g dbSNP:554713355
3555 3555 a, g dbSNP:759790063
3559 3559 c, g dbSNP:576229506
3588 3588 a, g dbSNP:376061969
3598 3598 g, t dbSNP:765548821
3602 3602 c, t dbSNP:752371775
3623 3623 a, c dbSNP:757990941
3681 3681 c, t dbSNP:565224641
3688 3688 c, g dbSNP:766153036
3706 3706 c, t dbSNP:76245756
3765 3765 g, t dbSNP:532686256
3768 3768 c, t dbSNP:184477204
3790 3790 a, c dbSNP:138312881
3802 3802 g, t dbSNP:751068883
3809 3809 a, g dbSNP:529983928
3822 3822 c, t dbSNP:757272448
3840 3840 a, g dbSNP:12106585
3942 3942 a, g dbSNP:17462054
3973 3973 c, t dbSNP:190590507
4024 4024 g, t dbSNP:530734498
4044 4044 a, g dbSNP:141268194
4054 4054 a, g dbSNP:73390944
4096 4096 c, t dbSNP:534745766
4097 4097 a, g dbSNP:546242180
4114 4114 a, g dbSNP:567737516
4168 4168 -, ttctg dbSNP:200967076
4172 4172 -, t, tt dbSNP:11442202
4182 4182 -, t dbSNP:397939715
4199 4199 c, g dbSNP:536498397
4203 4203 c, t dbSNP:193146795
4204 4204 g, t dbSNP:554850216
4227 4227 c, t dbSNP:144993615
4263 4263 c, t dbSNP:537244098
4275 4275 c, g dbSNP:559133039
4290 4290 a, g dbSNP:371637488
4309 4309 c, t dbSNP:550425263
4366 4366 c, t dbSNP:577249709
4371 4371 a, g dbSNP:541531112
4374 4374 a, g dbSNP:112175950
4385 4385 a, g dbSNP:5997506
4395 4395 c, g dbSNP:555359252
4399 4399 a, c dbSNP:773492147
4416 4416 c, t dbSNP:563567208
4417 4417 -, gct dbSNP:756940867
4437 4437 c, t dbSNP:77461085
4440 4440 c, t dbSNP:78535993
4445 4445 a, g dbSNP:771509575
4448 4448 a, t dbSNP:76646957
4460 4460 c, t dbSNP:777037756
4461 4461 a, g dbSNP:113956497
4493 4493 a, g dbSNP:534681348
4503 4503 c, g dbSNP:557684656
4515 4515 c, t dbSNP:765602116
4519 4519 c, t dbSNP:150581809
4558 4558 a, g dbSNP:114416141
4600 4600 c, t dbSNP:528145340
4605 4605 c, t dbSNP:73159090
4613 4613 a, c dbSNP:780221968
4614 4614 a, g dbSNP:185127651
4618 4618 c, g dbSNP:1034880
4637 4637 a, g dbSNP:550207111
4649 4649 c, t dbSNP:569992376
4701 4701 g, t dbSNP:537782319
4757 4757 c, t dbSNP:138606078
4774 4774 a, c dbSNP:768487274
4820 4820 c, t dbSNP:763727745
4827 4827 a, g dbSNP:565795130
4842 4842 a, t dbSNP:780919357
4903 4903 a, g dbSNP:199805259
4909 4909 a, g dbSNP:5763430
4914 4914 -, t dbSNP:771811332
4915 4915 -, t dbSNP:113760386
4930 4930 g, t dbSNP:77381275
4934 4934 a, g dbSNP:769634820
4954 4954 a, g dbSNP:189146105
4991 4991 c, t dbSNP:142797364
4998 4998 c, t dbSNP:575028212
5000 5000 c, t dbSNP:75293087
5010 5010 a, t dbSNP:76134776
5026 5026 c, t dbSNP:5763431
5034 5034 c, t dbSNP:181171324
5039 5039 c, t dbSNP:575435924
5072 5072 a, g dbSNP:112930471
5073 5073 c, t dbSNP:111641882
5075 5075 c, t dbSNP:564077209
5095 5095 g, t dbSNP:780709660
5107 5107 c, t dbSNP:74528592
5120 5120 a, g dbSNP:185938514
5123 5123 a, g dbSNP:190002811
5131 5131 c, t dbSNP:182522654
5196 5196 a, g dbSNP:750347453
5221 5221 a, g dbSNP:145080771
5228 5228 g, t dbSNP:117601743
5233 5233 c, t dbSNP:774123103
5283 5283 a, g dbSNP:527795536
5292 5292 a, g dbSNP:756019592
5316 5316 c, t dbSNP:764512220
5351 5351 c, t dbSNP:185621751
5358 5358 a, g dbSNP:117863523
5424 5424 a, g dbSNP:11090576
5429 5429 c, t dbSNP:369250167
5453 5453 a, g dbSNP:531083925
5463 5463 a, t dbSNP:188812769
5468 5468 -, g dbSNP:372162228
5477 5477 g, t dbSNP:552839202
5491 5491 a, c dbSNP:570917052
5555 5555 c, t dbSNP:533566312
5576 5576 a, t dbSNP:11537543
5617 5617 c, t dbSNP:535211691
5672 5672 -, t dbSNP:749974930
5703 5703 c, t dbSNP:553652217
5724 5724 c, t dbSNP:568548162
5725 5725 a, g dbSNP:41278851
5753 5753 a, c dbSNP:557359614
5760 5760 -, gcccaa dbSNP:755969033
5763 5763 -, a dbSNP:375908855
5794 5794 c, t dbSNP:372862060
5831 5831 c, t dbSNP:537735494
5840 5840 c, t dbSNP:556223075
5852 5852 c, t dbSNP:747215922

Target ORF information:

RefSeq Version NM_181828
Organism Homo sapiens (human)
Definition Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_181828

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGGTACTG
GATCATGATG TTTCAAAGGA AGAACCAGTC ACCTTTCACT TCTTGGCCAA ATTTTATCCT
GAGAATGCTG AAGAGGAGCT GGTTCAGGAG ATCACACAAC ATTTATTCTT CTTACAGGTA
AAGAAGCAGA TTTTAGATGA AAAGATCTAC TGCCCTCCTG AGGCTTCTGT GCTCCTGGCT
TCTTACGCCG TCCAGGCCAA GTATGGTGAC TACGACCCCA GTGTTCACAA GCGGGGATTT
TTGGCCCAAG AGGAATTGCT TCCAAAAAGG GTAATAAATC TGTATCAGAT GACTCCGGAA
ATGTGGGAGG AGAGAATTAC TGCTTGGTAC GCAGAGCACC GAGGCCGAGC CAGGGATGAA
GCTGAAATGG AATATCTGAA GATAGCTCAG GACCTGGAGA TGTACGGTGT GAACTACTTT
GCAATCCGGA ATAAAAAGGG CACAGAGCTG CTGCTTGGAG TGGATGCCCT GGGGCTTCAC
ATTTATGACC CTGAGAACAG ACTGACCCCC AAGATCTCCT TCCCGTGGAA TGAAATCCGA
AACATCTCGT ACAGTGACAA GGAGTTTACT ATTAAACCAC TGGATAAGAA AATTGATGTC
TTCAAGTTTA ACTCCTCAAA GCTTCGTGTT AATAAGCTGA TTCTCCAGCT ATGTATCGGG
AACCATGATC TATTTATGAG GAGAAGGAAA GCCGATTCTT TGGAAGTTCA GCAGATGAAA
GCCCAGGCCA GGGAGGAGAA GGCTAGAAAG CAGATGGAGC GGCAGCGCCT CGCTCGAGAG
AAGCAGATGA GGGAGGAGGC TGAACGCACG AGGGATGAGT TGGAGAGGAG GCTGCTGCAG
ATGAAAGAAG AAGCAACAAT GGCCAACGAA GCACTGATGC GGTCTGAGGA GACAGCTGAC
CTGTTGGCTG AAAAGGCCCA GATCACCGAG GAGGAGGCAA AACTTCTGGC CCAGAAGGCC
GCAGAGGCTG AGCAGGAAAT GCAGCGCATC AAGGCCACAG CGATTCGCAC GGAGGAGGAG
AAGCGCCTGA TGGAGCAGAA GGTGCTGGAA GCCGAGGTGC TGGCACTGAA GATGGCTGAG
GAGTCAGAGA GGAGGGCCAA AGAGGCAGAT CAGCTGAAGC AGGACCTGCA GGAAGCACGC
GAGGCGGAGC GAAGAGCCAA GCAGAAGCTC CTGGAGATTG CCACCAAGCC CACGTACCCG
CCCATGAACC CAATTCCAGC ACCGTTGCCT CCTGACATAC CAAGCTTCAA CCTCATTGGT
GACAGCCTGT CTTTCGACTT CAAAGATACT GACATGAAGC GGCTTTCCAT GGAGATAGAG
AAAGAAAAAG TGGAATACAT GGAAAAGAGC AAGCATCTGC AGGAGCAGCT CAATGAACTC
AAGACAGAAA TCGAGGCCTT GAAACTGAAA GAGAGGGAGA CAGCTCTGGA TATTCTGCAC
AATGAGAACT CCGACAGGGG TGGCAGCAGC AAGCACAATA CCATTAAAAA GCCTCAAGCC
CAAGGCAGAA GACCTATCTG CATTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16365
Accession Version NM_181829.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1650bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product merlin isoform 6
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN419318.1, CF138178.1, AF369664.1, AC004882.3 and BC071653.1. On Dec 20, 2007 this sequence version replaced gi:32967261. Summary: This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (6) lacks an alternate in-frame exon in the 5' coding region and includes an alternate exon in the 3' coding region, compared to variant 1. The resulting protein (isoform 6, also referred to as isoform delE3) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF369664.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGATGAAG
TGGAAAGGGA AGGACCTCTT TGATTTGGTG TGCCGGACTC TGGGGCTCCG AGAAACCTGG
TTCTTTGGAC TGCAGTACAC AATCAAGGAC ACAGTGGCCT GGCTCAAAAT GGACAAGAAG
GTAAAGAAGC AGATTTTAGA TGAAAAGATC TACTGCCCTC CTGAGGCTTC TGTGCTCCTG
GCTTCTTACG CCGTCCAGGC CAAGTATGGT GACTACGACC CCAGTGTTCA CAAGCGGGGA
TTTTTGGCCC AAGAGGAATT GCTTCCAAAA AGGGTAATAA ATCTGTATCA GATGACTCCG
GAAATGTGGG AGGAGAGAAT TACTGCTTGG TACGCAGAGC ACCGAGGCCG AGCCAGGGAT
GAAGCTGAAA TGGAATATCT GAAGATAGCT CAGGACCTGG AGATGTACGG TGTGAACTAC
TTTGCAATCC GGAATAAAAA GGGCACAGAG CTGCTGCTTG GAGTGGATGC CCTGGGGCTT
CACATTTATG ACCCTGAGAA CAGACTGACC CCCAAGATCT CCTTCCCGTG GAATGAAATC
CGAAACATCT CGTACAGTGA CAAGGAGTTT ACTATTAAAC CACTGGATAA GAAAATTGAT
GTCTTCAAGT TTAACTCCTC AAAGCTTCGT GTTAATAAGC TGATTCTCCA GCTATGTATC
GGGAACCATG ATCTATTTAT GAGGAGAAGG AAAGCCGATT CTTTGGAAGT TCAGCAGATG
AAAGCCCAGG CCAGGGAGGA GAAGGCTAGA AAGCAGATGG AGCGGCAGCG CCTCGCTCGA
GAGAAGCAGA TGAGGGAGGA GGCTGAACGC ACGAGGGATG AGTTGGAGAG GAGGCTGCTG
CAGATGAAAG AAGAAGCAAC AATGGCCAAC GAAGCACTGA TGCGGTCTGA GGAGACAGCT
GACCTGTTGG CTGAAAAGGC CCAGATCACC GAGGAGGAGG CAAAACTTCT GGCCCAGAAG
GCCGCAGAGG CTGAGCAGGA AATGCAGCGC ATCAAGGCCA CAGCGATTCG CACGGAGGAG
GAGAAGCGCC TGATGGAGCA GAAGGTGCTG GAAGCCGAGG TGCTGGCACT GAAGATGGCT
GAGGAGTCAG AGAGGAGGGC CAAAGAGGCA GATCAGCTGA AGCAGGACCT GCAGGAAGCA
CGCGAGGCGG AGCGAAGAGC CAAGCAGAAG CTCCTGGAGA TTGCCACCAA GCCCACGTAC
CCGCCCATGA ACCCAATTCC AGCACCGTTG CCTCCTGACA TACCAAGCTT CAACCTCATT
GGTGACAGCC TGTCTTTCGA CTTCAAAGAT ACTGACATGA AGCGGCTTTC CATGGAGATA
GAGAAAGAAA AAGTGGAATA CATGGAAAAG AGCAAGCATC TGCAGGAGCA GCTCAATGAA
CTCAAGACAG AAATCGAGGC CTTGAAACTG AAAGAGAGGG AGACAGCTCT GGATATTCTG
CACAATGAGA ACTCCGACAG GGGTGGCAGC AGCAAGCACA ATACCATTAA AAAGCCTCAA
GCCCAAGGCA GAAGACCTAT CTGCATTTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_861967.1
CDS444..2093
Misc Feature(1)357..359(+)
Misc Feature(2)510..986(+)
Misc Feature(3)519..719(+)
Misc Feature(4)684..962(+)
Misc Feature(5)966..1256(+)
Misc Feature(6)1092..1232(+)
Misc Feature(7)1092..1232(+)
Misc Feature(8)1200..1214(+)
Misc Feature(9)1209..1256(+)
Misc Feature(10)1281..1943(+)
Misc Feature(11)1359..2057(+)
Misc Feature(12)1872..1874(+)
Misc Feature(13)1872..1874(+)
Exon (1)1..557
Gene:NF2
Gene Synonym:
Exon (2)558..683
Gene:NF2
Gene Synonym:
Exon (3)684..767
Gene:NF2
Gene Synonym:
Exon (4)768..836
Gene:NF2
Gene Synonym:
Exon (5)837..919
Gene:NF2
Gene Synonym:
Exon (6)920..995
Gene:NF2
Gene Synonym:
Exon (7)996..1130
Gene:NF2
Gene Synonym:
Exon (8)1131..1205
Gene:NF2
Gene Synonym:
Exon (9)1206..1319
Gene:NF2
Gene Synonym:
Exon (10)1320..1442
Gene:NF2
Gene Synonym:
Exon (11)1443..1660
Gene:NF2
Gene Synonym:
Exon (12)1661..1766
Gene:NF2
Gene Synonym:
Exon (13)1767..1894
Gene:NF2
Gene Synonym:
Exon (14)1895..2057
Gene:NF2
Gene Synonym:
Exon (15)2058..2102
Gene:NF2
Gene Synonym:
Exon (16)2103..5951
Gene:NF2
Gene Synonym:
Translation
Position Chain Variation Link
40 40 c, t dbSNP:570135699
55 55 c, t dbSNP:539283977
56 56 a, g dbSNP:777179356
103 103 a, c dbSNP:571842282
114 114 g, t dbSNP:554177413
198 198 c, g dbSNP:56733822
199 199 c, g dbSNP:536438675
208 208 c, t dbSNP:555236806
240 240 a, c dbSNP:1800539
334 334 c, g dbSNP:1800540
341 341 c, g dbSNP:1042230
360 360 c, g dbSNP:558960493
396 396 a, c dbSNP:368022063
398 398 a, g dbSNP:753401671
405 405 c, g dbSNP:3181682
416 416 c, t dbSNP:763602804
425 425 c, t dbSNP:577180052
426 426 a, g dbSNP:201591536
427 427 a, g dbSNP:748043566
430 430 c, t dbSNP:758236169
435 435 c, t dbSNP:777578586
436 436 c, t dbSNP:746741839
442 442 c, g dbSNP:559386105
455 455 c, t dbSNP:144477078
466 466 c, g dbSNP:775564806
467 467 a, c dbSNP:749452887
479 479 c, t dbSNP:371800843
491 491 a, c, g dbSNP:774973059
500 500 c, g dbSNP:541641232
514 514 c, t dbSNP:773714780
515 515 c, g dbSNP:761062232
532 532 a, g dbSNP:563168478
537 537 a, g dbSNP:373337083
543 543 c, g dbSNP:753425376
550 550 a, g dbSNP:372279458
558 558 a, g dbSNP:761188569
568 568 -, g dbSNP:587776564
576 576 g, t dbSNP:370147621
578 578 c, t dbSNP:752664255
588 588 a, t dbSNP:759129920
597 597 c, t dbSNP:764901064
603 603 c, t dbSNP:752220604
604 604 g, t dbSNP:762440698
606 606 a, g dbSNP:764089682
612 612 c, t dbSNP:121434259
613 613 a, g dbSNP:368773485
620 620 c, t dbSNP:757125309
623 623 c, g, t dbSNP:780872661
628 628 c, t dbSNP:121434261
629 629 c, t dbSNP:373178836
633 633 c, t dbSNP:779218238
637 637 a, g dbSNP:541636141
639 639 a, t dbSNP:772274240
644 644 a, g dbSNP:557924117
645 645 a, g dbSNP:778460074
647 647 c, t dbSNP:747447569
648 648 -, aaggaca dbSNP:587776565
651 651 g, t dbSNP:559558673
675 675 a, g dbSNP:17851784
686 686 a, g dbSNP:750431087
688 688 a, g dbSNP:139779327
696 696 a, g dbSNP:780483061
716 716 c, t dbSNP:749708226
718 718 c, g dbSNP:759344163
725 725 a, t dbSNP:778469232
746 746 g, t dbSNP:747813808
756 756 a, g dbSNP:771572024
779 779 c, t dbSNP:142972020
785 785 c, t dbSNP:374911526
798 798 c, t dbSNP:150667239
817 817 a, t dbSNP:779353677
824 824 a, g dbSNP:182990439
835 835 a, g dbSNP:752963731
846 846 c, t dbSNP:756922719
851 851 c, t dbSNP:780880330
858 858 a, g dbSNP:745315897
863 863 a, g dbSNP:139838280
864 864 g, t dbSNP:74315495
896 896 c, t dbSNP:149803133
900 900 g, t dbSNP:374494685
907 907 a, g dbSNP:749176138
933 933 a, g dbSNP:141629512
934 934 c, t dbSNP:747871414
971 971 c, t dbSNP:367802186
972 972 a, g dbSNP:776818377
982 982 a, g dbSNP:746025177
994 994 a, g dbSNP:543087642
1003 1003 a, g dbSNP:145384260
1011 1011 c, g dbSNP:770019352
1019 1019 g, t dbSNP:780003734
1025 1025 a, c dbSNP:749331641
1033 1033 c, t dbSNP:761195572
1040 1040 g, t dbSNP:769348972
1044 1044 a, c dbSNP:771371228
1047 1047 a, g dbSNP:774996651
1058 1058 c, t dbSNP:147665163
1068 1068 c, t dbSNP:772540432
1078 1078 a, g dbSNP:773573049
1079 1079 a, g dbSNP:760272926
1082 1082 a, c dbSNP:765985886
1089 1089 c, t dbSNP:777059212
1090 1090 c, t dbSNP:753300935
1091 1091 a, g dbSNP:759032771
1103 1103 c, t dbSNP:765223726
1104 1104 c, t dbSNP:74315496
1115 1115 a, g dbSNP:376609988
1146 1146 c, t dbSNP:764702893
1147 1147 c, t dbSNP:549225513
1151 1151 g, t dbSNP:762883753
1171 1171 a, g, t dbSNP:764034925
1177 1177 a, g dbSNP:757074151
1181 1181 c, t dbSNP:143160499
1187 1187 a, g dbSNP:766554538
1191 1191 c, t dbSNP:754093587
1192 1192 a, g dbSNP:755200117
1199 1199 c, t dbSNP:151198477
1202 1202 a, g dbSNP:146166875
1211 1211 c, t dbSNP:755253391
1217 1217 a, g dbSNP:569956351
1221 1221 a, g dbSNP:752879150
1223 1223 c, t dbSNP:199957176
1232 1232 a, c, t dbSNP:200272173
1259 1259 c, t dbSNP:747526252
1267 1267 g, t dbSNP:750633919
1268 1268 g, t dbSNP:373606097
1278 1278 c, t dbSNP:74315497
1286 1286 a, g, t dbSNP:746175548
1289 1289 c, t dbSNP:775119805
1290 1290 c, t dbSNP:74315500
1297 1297 a, g dbSNP:748792522
1301 1301 a, g dbSNP:768194921
1303 1303 a, t dbSNP:200372028
1315 1315 -, a dbSNP:587776563
1326 1326 c, t dbSNP:140266312
1327 1327 a, g dbSNP:587778554
1332 1332 c, t dbSNP:761795291
1333 1333 a, g dbSNP:768053145
1334 1334 c, t dbSNP:750911082
1337 1337 c, t dbSNP:756568857
1338 1338 a, g dbSNP:780430071
1341 1341 c, t dbSNP:74315499
1342 1342 a, g, t dbSNP:754087071
1352 1352 c, g dbSNP:2229064
1368 1368 a, g dbSNP:778412102
1371 1371 c, t dbSNP:747756728
1372 1372 a, g dbSNP:771675702
1375 1375 c, t dbSNP:764441073
1376 1376 a, g, t dbSNP:145427713
1394 1394 c, g dbSNP:770949972
1399 1399 c, t dbSNP:74315492
1404 1404 c, t dbSNP:74315498
1407 1407 a, g dbSNP:776499914
1426 1426 c, t dbSNP:745637107
1432 1432 a, g dbSNP:577940601
1433 1433 c, t dbSNP:142459414
1437 1437 a, g dbSNP:761933185
1448 1448 c, g dbSNP:757322524
1459 1459 c, g dbSNP:780987048
1475 1475 c, t dbSNP:745717285
1483 1483 c, t dbSNP:587778553
1493 1493 c, t dbSNP:769454739
1526 1526 c, t dbSNP:137953976
1528 1528 c, t dbSNP:546551743
1532 1532 a, g dbSNP:748403377
1535 1535 g, t dbSNP:772219612
1539 1539 c, t dbSNP:74315501
1551 1551 c, t dbSNP:773296925
1552 1552 a, g dbSNP:201214090
1557 1557 a, g dbSNP:766974263
1563 1563 a, c dbSNP:776970251
1568 1568 a, g dbSNP:759993776
1572 1572 c, t dbSNP:765540111
1573 1573 a, g dbSNP:548217466
1577 1577 c, g dbSNP:375969005
1578 1578 a, g dbSNP:758128105
1590 1590 c, t dbSNP:763826793
1591 1591 a, g, t dbSNP:751182657
1592 1592 a, c dbSNP:781241303
1593 1593 c, t dbSNP:142446775
1600 1600 a, g dbSNP:760382219
1601 1601 a, g dbSNP:113715304
1610 1610 a, g dbSNP:756041768
1618 1618 c, t dbSNP:779721863
1619 1619 c, t dbSNP:749028891
1623 1623 g, t dbSNP:772334382
1631 1631 a, g dbSNP:144628209
1632 1632 c, t dbSNP:747129449
1694 1694 g, t dbSNP:747252414
1704 1704 c, t dbSNP:771143279
1705 1705 a, g dbSNP:373650983
1706 1706 c, t dbSNP:138354622
1707 1707 a, g, t dbSNP:74315503
1711 1711 c, t dbSNP:776109136
1712 1712 a, g dbSNP:375819833
1716 1716 c, t dbSNP:74315504
1736 1736 c, t dbSNP:148776784
1745 1745 a, t dbSNP:774818801
1750 1750 c, t dbSNP:761378464
1755 1755 c, t dbSNP:768587800
1759 1759 c, t dbSNP:145666157
1760 1760 a, g dbSNP:774373946
1763 1763 c, t dbSNP:760224815
1765 1765 c, t dbSNP:766339217
1766 1766 a, g dbSNP:753751373
1768 1768 c, t dbSNP:771692777
1771 1771 c, t dbSNP:141538143
1775 1775 c, t dbSNP:368966931
1779 1779 a, g dbSNP:147506929
1788 1788 c, g, t dbSNP:776076922
1789 1789 c, t dbSNP:765100922
1790 1790 a, g, t dbSNP:373442542
1820 1820 c, t dbSNP:758182356
1822 1822 c, t dbSNP:767682136
1825 1825 a, g dbSNP:552115565
1831 1831 g, t dbSNP:756366940
1838 1838 a, t dbSNP:780287551
1842 1842 a, g dbSNP:749326764
1852 1852 a, t dbSNP:140025001
1860 1860 a, g dbSNP:201527155
1866 1866 c, t dbSNP:762588182
1868 1868 g, t dbSNP:534398531
1899 1899 g, t dbSNP:74315505
1901 1901 a, t dbSNP:770627371
1906 1906 c, t dbSNP:780818183
1918 1918 a, g dbSNP:745432431
1920 1920 c, t dbSNP:769370159
1924 1924 c, t dbSNP:74315493
1933 1933 a, c dbSNP:74315494
1939 1939 a, g dbSNP:774824164
1950 1950 a, g dbSNP:762953921
1953 1953 c, g dbSNP:768422432
1958 1958 c, t dbSNP:774264046
1959 1959 a, g dbSNP:199669486
1987 1987 c, t dbSNP:766752048
1989 1989 a, g dbSNP:754087219
1991 1991 c, t dbSNP:759780271
1992 1992 c, g dbSNP:765386271
1998 1998 a, g, t dbSNP:557347747
2003 2003 a, g dbSNP:758955756
2004 2004 c, t dbSNP:778127814
2008 2008 a, g dbSNP:768303416
2018 2018 c, t dbSNP:751987156
2019 2019 a, g dbSNP:757586383
2022 2022 -, ag dbSNP:755032702
2026 2026 a, g dbSNP:781488145
2071 2071 a, g dbSNP:746764835
2082 2082 a, g dbSNP:187478738
2096 2096 c, t dbSNP:770119441
2103 2103 c, t dbSNP:749475992
2108 2108 c, t dbSNP:768807694
2114 2114 a, g dbSNP:201911915
2116 2116 a, g dbSNP:761874138
2117 2117 c, t dbSNP:370999558
2118 2118 a, g dbSNP:145446060
2125 2125 c, t dbSNP:761191666
2128 2128 a, g dbSNP:766689587
2132 2132 a, g dbSNP:754168138
2136 2136 c, t dbSNP:759278621
2139 2139 c, t dbSNP:764972504
2157 2157 a, g dbSNP:141099051
2160 2160 a, c dbSNP:757952483
2162 2162 a, c dbSNP:777907253
2163 2163 c, g, t dbSNP:751474583
2168 2168 a, c dbSNP:780862097
2176 2176 a, c dbSNP:200740197
2181 2181 c, g dbSNP:768930578
2187 2187 -, ctc dbSNP:776194172
2189 2189 c, t dbSNP:536441436
2191 2191 c, t dbSNP:748272771
2195 2195 a, t dbSNP:772158241
2196 2196 c, g dbSNP:773299250
2197 2197 a, c, t dbSNP:374219104
2198 2198 a, g dbSNP:377393360
2201 2201 a, g dbSNP:759977916
2203 2203 c, t dbSNP:765024618
2204 2204 g, t dbSNP:554849830
2205 2205 a, c, t dbSNP:370016332
2206 2206 a, g dbSNP:751023311
2218 2218 a, g dbSNP:746841804
2223 2223 a, g dbSNP:754084562
2278 2278 c, t dbSNP:537177702
2285 2285 a, c dbSNP:3180505
2313 2313 g, t dbSNP:558494389
2342 2342 a, c dbSNP:754717476
2386 2386 c, t dbSNP:778408052
2410 2410 a, g dbSNP:531470040
2412 2412 c, g dbSNP:545104019
2433 2433 c, t dbSNP:576785860
2489 2489 a, g dbSNP:373511643
2507 2507 c, t dbSNP:1008515
2512 2512 a, g dbSNP:572307337
2541 2541 c, t dbSNP:777219109
2573 2573 c, t dbSNP:541235064
2603 2603 c, g dbSNP:557890822
2629 2629 c, t dbSNP:577882884
2656 2656 c, t dbSNP:191509325
2690 2690 c, t dbSNP:542811466
2714 2714 c, t dbSNP:776610927
2726 2726 c, t dbSNP:543669369
2727 2727 a, g dbSNP:561178202
2731 2731 c, t dbSNP:531658450
2742 2742 c, g dbSNP:770711462
2784 2784 a, g dbSNP:201571578
2798 2798 a, g dbSNP:543529572
2800 2800 a, g dbSNP:781112228
2857 2857 -, a dbSNP:567370520
2869 2869 a, g dbSNP:565234426
2873 2873 a, g dbSNP:184043147
2898 2898 -, c dbSNP:761028781
2946 2946 c, t dbSNP:562778105
2947 2947 a, g dbSNP:374557247
2985 2985 a, g dbSNP:186559222
3029 3029 c, t dbSNP:149840456
3087 3087 c, g dbSNP:145820299
3110 3110 c, t dbSNP:531577838
3127 3127 c, t dbSNP:8140096
3143 3143 a, g dbSNP:569883007
3229 3229 a, c dbSNP:762085797
3240 3240 c, t dbSNP:148973148
3252 3252 c, g dbSNP:773367316
3270 3270 c, t dbSNP:191183183
3293 3293 g, t dbSNP:145719271
3342 3342 c, t dbSNP:534317366
3345 3345 a, g dbSNP:761248952
3361 3361 c, t dbSNP:2530680
3373 3373 c, t dbSNP:572384896
3423 3423 c, t dbSNP:542927614
3470 3470 -, gtggga dbSNP:148703036
3521 3521 g, t dbSNP:754278601
3529 3529 a, g dbSNP:554713355
3558 3558 a, g dbSNP:759790063
3562 3562 c, g dbSNP:576229506
3591 3591 a, g dbSNP:376061969
3601 3601 g, t dbSNP:765548821
3605 3605 c, t dbSNP:752371775
3626 3626 a, c dbSNP:757990941
3684 3684 c, t dbSNP:565224641
3691 3691 c, g dbSNP:766153036
3709 3709 c, t dbSNP:76245756
3768 3768 g, t dbSNP:532686256
3771 3771 c, t dbSNP:184477204
3793 3793 a, c dbSNP:138312881
3805 3805 g, t dbSNP:751068883
3812 3812 a, g dbSNP:529983928
3825 3825 c, t dbSNP:757272448
3843 3843 a, g dbSNP:12106585
3945 3945 a, g dbSNP:17462054
3976 3976 c, t dbSNP:190590507
4027 4027 g, t dbSNP:530734498
4047 4047 a, g dbSNP:141268194
4057 4057 a, g dbSNP:73390944
4099 4099 c, t dbSNP:534745766
4100 4100 a, g dbSNP:546242180
4117 4117 a, g dbSNP:567737516
4171 4171 -, ttctg dbSNP:200967076
4175 4175 -, t, tt dbSNP:11442202
4185 4185 -, t dbSNP:397939715
4202 4202 c, g dbSNP:536498397
4206 4206 c, t dbSNP:193146795
4207 4207 g, t dbSNP:554850216
4230 4230 c, t dbSNP:144993615
4266 4266 c, t dbSNP:537244098
4278 4278 c, g dbSNP:559133039
4293 4293 a, g dbSNP:371637488
4312 4312 c, t dbSNP:550425263
4369 4369 c, t dbSNP:577249709
4374 4374 a, g dbSNP:541531112
4377 4377 a, g dbSNP:112175950
4388 4388 a, g dbSNP:5997506
4398 4398 c, g dbSNP:555359252
4402 4402 a, c dbSNP:773492147
4419 4419 c, t dbSNP:563567208
4420 4420 -, gct dbSNP:756940867
4440 4440 c, t dbSNP:77461085
4443 4443 c, t dbSNP:78535993
4448 4448 a, g dbSNP:771509575
4451 4451 a, t dbSNP:76646957
4463 4463 c, t dbSNP:777037756
4464 4464 a, g dbSNP:113956497
4496 4496 a, g dbSNP:534681348
4506 4506 c, g dbSNP:557684656
4518 4518 c, t dbSNP:765602116
4522 4522 c, t dbSNP:150581809
4561 4561 a, g dbSNP:114416141
4603 4603 c, t dbSNP:528145340
4608 4608 c, t dbSNP:73159090
4616 4616 a, c dbSNP:780221968
4617 4617 a, g dbSNP:185127651
4621 4621 c, g dbSNP:1034880
4640 4640 a, g dbSNP:550207111
4652 4652 c, t dbSNP:569992376
4704 4704 g, t dbSNP:537782319
4760 4760 c, t dbSNP:138606078
4777 4777 a, c dbSNP:768487274
4823 4823 c, t dbSNP:763727745
4830 4830 a, g dbSNP:565795130
4845 4845 a, t dbSNP:780919357
4906 4906 a, g dbSNP:199805259
4912 4912 a, g dbSNP:5763430
4917 4917 -, t dbSNP:771811332
4918 4918 -, t dbSNP:113760386
4933 4933 g, t dbSNP:77381275
4937 4937 a, g dbSNP:769634820
4957 4957 a, g dbSNP:189146105
4994 4994 c, t dbSNP:142797364
5001 5001 c, t dbSNP:575028212
5003 5003 c, t dbSNP:75293087
5013 5013 a, t dbSNP:76134776
5029 5029 c, t dbSNP:5763431
5037 5037 c, t dbSNP:181171324
5042 5042 c, t dbSNP:575435924
5075 5075 a, g dbSNP:112930471
5076 5076 c, t dbSNP:111641882
5078 5078 c, t dbSNP:564077209
5098 5098 g, t dbSNP:780709660
5110 5110 c, t dbSNP:74528592
5123 5123 a, g dbSNP:185938514
5126 5126 a, g dbSNP:190002811
5134 5134 c, t dbSNP:182522654
5199 5199 a, g dbSNP:750347453
5224 5224 a, g dbSNP:145080771
5231 5231 g, t dbSNP:117601743
5236 5236 c, t dbSNP:774123103
5286 5286 a, g dbSNP:527795536
5295 5295 a, g dbSNP:756019592
5319 5319 c, t dbSNP:764512220
5354 5354 c, t dbSNP:185621751
5361 5361 a, g dbSNP:117863523
5427 5427 a, g dbSNP:11090576
5432 5432 c, t dbSNP:369250167
5456 5456 a, g dbSNP:531083925
5466 5466 a, t dbSNP:188812769
5471 5471 -, g dbSNP:372162228
5480 5480 g, t dbSNP:552839202
5494 5494 a, c dbSNP:570917052
5558 5558 c, t dbSNP:533566312
5579 5579 a, t dbSNP:11537543
5620 5620 c, t dbSNP:535211691
5675 5675 -, t dbSNP:749974930
5706 5706 c, t dbSNP:553652217
5727 5727 c, t dbSNP:568548162
5728 5728 a, g dbSNP:41278851
5756 5756 a, c dbSNP:557359614
5763 5763 -, gcccaa dbSNP:755969033
5766 5766 -, a dbSNP:375908855
5797 5797 c, t dbSNP:372862060
5834 5834 c, t dbSNP:537735494
5843 5843 c, t dbSNP:556223075
5855 5855 c, t dbSNP:747215922

Target ORF information:

RefSeq Version NM_181829
Organism Homo sapiens (human)
Definition Homo sapiens neurofibromin 2 (merlin) (NF2), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_181829

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGATGAAG
TGGAAAGGGA AGGACCTCTT TGATTTGGTG TGCCGGACTC TGGGGCTCCG AGAAACCTGG
TTCTTTGGAC TGCAGTACAC AATCAAGGAC ACAGTGGCCT GGCTCAAAAT GGACAAGAAG
GTAAAGAAGC AGATTTTAGA TGAAAAGATC TACTGCCCTC CTGAGGCTTC TGTGCTCCTG
GCTTCTTACG CCGTCCAGGC CAAGTATGGT GACTACGACC CCAGTGTTCA CAAGCGGGGA
TTTTTGGCCC AAGAGGAATT GCTTCCAAAA AGGGTAATAA ATCTGTATCA GATGACTCCG
GAAATGTGGG AGGAGAGAAT TACTGCTTGG TACGCAGAGC ACCGAGGCCG AGCCAGGGAT
GAAGCTGAAA TGGAATATCT GAAGATAGCT CAGGACCTGG AGATGTACGG TGTGAACTAC
TTTGCAATCC GGAATAAAAA GGGCACAGAG CTGCTGCTTG GAGTGGATGC CCTGGGGCTT
CACATTTATG ACCCTGAGAA CAGACTGACC CCCAAGATCT CCTTCCCGTG GAATGAAATC
CGAAACATCT CGTACAGTGA CAAGGAGTTT ACTATTAAAC CACTGGATAA GAAAATTGAT
GTCTTCAAGT TTAACTCCTC AAAGCTTCGT GTTAATAAGC TGATTCTCCA GCTATGTATC
GGGAACCATG ATCTATTTAT GAGGAGAAGG AAAGCCGATT CTTTGGAAGT TCAGCAGATG
AAAGCCCAGG CCAGGGAGGA GAAGGCTAGA AAGCAGATGG AGCGGCAGCG CCTCGCTCGA
GAGAAGCAGA TGAGGGAGGA GGCTGAACGC ACGAGGGATG AGTTGGAGAG GAGGCTGCTG
CAGATGAAAG AAGAAGCAAC AATGGCCAAC GAAGCACTGA TGCGGTCTGA GGAGACAGCT
GACCTGTTGG CTGAAAAGGC CCAGATCACC GAGGAGGAGG CAAAACTTCT GGCCCAGAAG
GCCGCAGAGG CTGAGCAGGA AATGCAGCGC ATCAAGGCCA CAGCGATTCG CACGGAGGAG
GAGAAGCGCC TGATGGAGCA GAAGGTGCTG GAAGCCGAGG TGCTGGCACT GAAGATGGCT
GAGGAGTCAG AGAGGAGGGC CAAAGAGGCA GATCAGCTGA AGCAGGACCT GCAGGAAGCA
CGCGAGGCGG AGCGAAGAGC CAAGCAGAAG CTCCTGGAGA TTGCCACCAA GCCCACGTAC
CCGCCCATGA ACCCAATTCC AGCACCGTTG CCTCCTGACA TACCAAGCTT CAACCTCATT
GGTGACAGCC TGTCTTTCGA CTTCAAAGAT ACTGACATGA AGCGGCTTTC CATGGAGATA
GAGAAAGAAA AAGTGGAATA CATGGAAAAG AGCAAGCATC TGCAGGAGCA GCTCAATGAA
CTCAAGACAG AAATCGAGGC CTTGAAACTG AAAGAGAGGG AGACAGCTCT GGATATTCTG
CACAATGAGA ACTCCGACAG GGGTGGCAGC AGCAAGCACA ATACCATTAA AAAGCCTCAA
GCCCAAGGCA GAAGACCTAT CTGCATTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16489
Accession Version NM_181830.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1524bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu16489D_COA.pdf (pdf)
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product merlin isoform 7
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN419318.1, AF369657.1, AF369665.1, AC004882.3 and BC071653.1. On Dec 20, 2007 this sequence version replaced gi:32967263. Summary: This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (7) lacks two alternate in-frame exons in the 5' coding region and includes an alternate exon in the 3' coding region, compared to variant 1. The resulting protein (isoform 7, also referred to as isoform delE2/3) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF369665.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGGGG CCATCGCTTC CCGCATGAGC TTCAGCTCTC TCAAGAGGAA GCAACCCAAG 
ACGTTCACCG TGAGGATCGT CACCATGGAC GCCGAGATGG AGTTCAATTG CGAGGTAAAG
AAGCAGATTT TAGATGAAAA GATCTACTGC CCTCCTGAGG CTTCTGTGCT CCTGGCTTCT
TACGCCGTCC AGGCCAAGTA TGGTGACTAC GACCCCAGTG TTCACAAGCG GGGATTTTTG
GCCCAAGAGG AATTGCTTCC AAAAAGGGTA ATAAATCTGT ATCAGATGAC TCCGGAAATG
TGGGAGGAGA GAATTACTGC TTGGTACGCA GAGCACCGAG GCCGAGCCAG GGATGAAGCT
GAAATGGAAT ATCTGAAGAT AGCTCAGGAC CTGGAGATGT ACGGTGTGAA CTACTTTGCA
ATCCGGAATA AAAAGGGCAC AGAGCTGCTG CTTGGAGTGG ATGCCCTGGG GCTTCACATT
TATGACCCTG AGAACAGACT GACCCCCAAG ATCTCCTTCC CGTGGAATGA AATCCGAAAC
ATCTCGTACA GTGACAAGGA GTTTACTATT AAACCACTGG ATAAGAAAAT TGATGTCTTC
AAGTTTAACT CCTCAAAGCT TCGTGTTAAT AAGCTGATTC TCCAGCTATG TATCGGGAAC
CATGATCTAT TTATGAGGAG AAGGAAAGCC GATTCTTTGG AAGTTCAGCA GATGAAAGCC
CAGGCCAGGG AGGAGAAGGC TAGAAAGCAG ATGGAGCGGC AGCGCCTCGC TCGAGAGAAG
CAGATGAGGG AGGAGGCTGA ACGCACGAGG GATGAGTTGG AGAGGAGGCT GCTGCAGATG
AAAGAAGAAG CAACAATGGC CAACGAAGCA CTGATGCGGT CTGAGGAGAC AGCTGACCTG
TTGGCTGAAA AGGCCCAGAT CACCGAGGAG GAGGCAAAAC TTCTGGCCCA GAAGGCCGCA
GAGGCTGAGC AGGAAATGCA GCGCATCAAG GCCACAGCGA TTCGCACGGA GGAGGAGAAG
CGCCTGATGG AGCAGAAGGT GCTGGAAGCC GAGGTGCTGG CACTGAAGAT GGCTGAGGAG
TCAGAGAGGA GGGCCAAAGA GGCAGATCAG CTGAAGCAGG ACCTGCAGGA AGCACGCGAG
GCGGAGCGAA GAGCCAAGCA GAAGCTCCTG GAGATTGCCA CCAAGCCCAC GTACCCGCCC
ATGAACCCAA TTCCAGCACC GTTGCCTCCT GACATACCAA GCTTCAACCT CATTGGTGAC
AGCCTGTCTT TCGACTTCAA AGATACTGAC ATGAAGCGGC TTTCCATGGA GATAGAGAAA
GAAAAAGTGG AATACATGGA AAAGAGCAAG CATCTGCAGG AGCAGCTCAA TGAACTCAAG
ACAGAAATCG AGGCCTTGAA ACTGAAAGAG AGGGAGACAG CTCTGGATAT TCTGCACAAT
GAGAACTCCG ACAGGGGTGG CAGCAGCAAG CACAATACCA TTAAAAAGCC TCAAGCCCAA
GGCAGAAGAC CTATCTGCAT TTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_861968.1
CDS444..1967
Misc Feature(1)357..359(+)
Misc Feature(2)552..860(+)
Misc Feature(3)558..836(+)
Misc Feature(4)840..1130(+)
Misc Feature(5)966..1106(+)
Misc Feature(6)966..1106(+)
Misc Feature(7)1074..1088(+)
Misc Feature(8)1083..1130(+)
Misc Feature(9)1155..1817(+)
Misc Feature(10)1233..1931(+)
Misc Feature(11)1746..1748(+)
Misc Feature(12)1746..1748(+)
Exon (1)1..557
Gene:NF2
Gene Synonym:
Exon (2)558..641
Gene:NF2
Gene Synonym:
Exon (3)642..710
Gene:NF2
Gene Synonym:
Exon (4)711..793
Gene:NF2
Gene Synonym:
Exon (5)794..869
Gene:NF2
Gene Synonym:
Exon (6)870..1004
Gene:NF2
Gene Synonym:
Exon (7)1005..1079
Gene:NF2
Gene Synonym:
Exon (8)1080..1193
Gene:NF2
Gene Synonym:
Exon (9)1194..1316
Gene:NF2
Gene Synonym:
Exon (10)1317..1534
Gene:NF2
Gene Synonym:
Exon (11)1535..1640
Gene:NF2
Gene Synonym:
Exon (12)1641..1768
Gene:NF2
Gene Synonym:
Exon (13)1769..1931
Gene:NF2
Gene Synonym:
Exon (14)1932..1976
Gene:NF2
Gene Synonym:
Exon (15)1977..5825
Gene:NF2
Gene Synonym:
Translation
Position Chain Variation Link
40 40 c, t dbSNP:570135699
55 55 c, t dbSNP:539283977
56 56 a, g dbSNP:777179356
103 103 a, c dbSNP:571842282
114 114 g, t dbSNP:554177413
198 198 c, g dbSNP:56733822
199 199 c, g dbSNP:536438675
208 208 c, t dbSNP:555236806
240 240 a, c dbSNP:1800539
334 334 c, g dbSNP:1800540
341 341 c, g dbSNP:1042230
360 360 c, g dbSNP:558960493
396 396 a, c dbSNP:368022063
398 398 a, g dbSNP:753401671
405 405 c, g dbSNP:3181682
416 416 c, t dbSNP:763602804
425 425 c, t dbSNP:577180052
426 426 a, g dbSNP:201591536
427 427 a, g dbSNP:748043566
430 430 c, t dbSNP:758236169
435 435 c, t dbSNP:777578586
436 436 c, t dbSNP:746741839
442 442 c, g dbSNP:559386105
455 455 c, t dbSNP:144477078
466 466 c, g dbSNP:775564806
467 467 a, c dbSNP:749452887
479 479 c, t dbSNP:371800843
491 491 a, c, g dbSNP:774973059
500 500 c, g dbSNP:541641232
514 514 c, t dbSNP:773714780
515 515 c, g dbSNP:761062232
532 532 a, g dbSNP:563168478
537 537 a, g dbSNP:373337083
543 543 c, g dbSNP:753425376
550 550 a, g dbSNP:372279458
560 560 a, g dbSNP:750431087
562 562 a, g dbSNP:139779327
570 570 a, g dbSNP:780483061
590 590 c, t dbSNP:749708226
592 592 c, g dbSNP:759344163
599 599 a, t dbSNP:778469232
620 620 g, t dbSNP:747813808
630 630 a, g dbSNP:771572024
653 653 c, t dbSNP:142972020
659 659 c, t dbSNP:374911526
672 672 c, t dbSNP:150667239
691 691 a, t dbSNP:779353677
698