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NFKBIA cDNA ORF clone, Homo sapiens (human)

Gene Symbol NFKBIA
Entrez Gene ID 4792
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Synonyms IKBA, MAD-3, NFKBI
General protein information
Preferred Names
NF-kappa-B inhibitor alpha
Names
NF-kappa-B inhibitor alpha
ikB-alpha
IkappaBalpha
I-kappa-B-alpha
nuclear factor of kappa light chain gene enhancer in B-cells
major histocompatibility complex enhancer-binding protein MAD3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

14

14q13

Summary This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]. lac of sum
Disorder MIM:

164008

Disorder Html: Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency,

mRNA and Protein(s)

mRNA Protein Name
NM_020529 NP_065390 NF-kappa-B inhibitor alpha

KEGG
hsa04210 Apoptosis
hsa04660 T cell receptor signaling pathway
hsa04620 Toll-like receptor signaling pathway
hsa04662 B cell receptor signaling pathway
hsa05131 Shigellosis
hsa04920 Adipocytokine signaling pathway
hsa05222 Small cell lung cancer
hsa05120 Epithelial cell signaling in Helicobacter pylori infection
hsa05220 Chronic myeloid leukemia
hsa05215 Prostate cancer
hsa05200 Pathways in cancer
hsa04722 Neurotrophin signaling pathway
hsa04621 NOD-like receptor signaling pathway
hsa04622 RIG-I-like receptor signaling pathway
hsa04623 Cytosolic DNA-sensing pathway
hsa04062 Chemokine signaling pathway
hsa05142 Chagas disease (American trypanosomiasis)
hsa05140 Leishmaniasis
hsa05145 Toxoplasmosis
hsa05160 Hepatitis C
hsa04380 Osteoclast differentiation
hsa05162 Measles
hsa05166 HTLV-I infection
hsa05164 Influenza A
hsa05168 Herpes simplex infection
hsa05134 Legionellosis
hsa05203 Viral carcinogenesis
hsa05161 Hepatitis B
hsa05169 Epstein-Barr virus infection
hsa04064 NF-kappa B signaling pathway
hsa04024 cAMP signaling pathway
hsa04668 TNF signaling pathway
Reactome
R-HSA-1643685 Disease
R-HSA-5602358 Diseases associated with the TLR signaling cascade
R-HSA-168256 Immune System
R-HSA-1280218 Adaptive Immune System
R-HSA-5603027 IKBKG deficiency causes anhidrotic ectodermal dysplasia with immunodeficiency (EDA-ID) (via TLR)
R-HSA-5260271 Diseases of Immune System
R-HSA-5603029 IkBA variant leads to EDA-ID
R-HSA-1168372 Downstream signaling events of B Cell Receptor (BCR)
R-HSA-202424 Downstream TCR signaling
R-HSA-983705 Signaling by the B Cell Receptor (BCR)
R-HSA-1169091 Activation of NF-kappaB in B cells
R-HSA-202403 TCR signaling
R-HSA-445989 TAK1 activates NFkB by phosphorylation and activation of IKKs complex
R-HSA-168164 Toll Like Receptor 3 (TLR3) Cascade
R-HSA-166166 MyD88-independent TLR3/TLR4 cascade
R-HSA-975871 MyD88 cascade initiated on plasma membrane
R-HSA-168176 Toll Like Receptor 5 (TLR5) Cascade
R-HSA-168898 Toll-Like Receptors Cascades
R-HSA-168142 Toll Like Receptor 10 (TLR10) Cascade
R-HSA-975138 TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation
R-HSA-168249 Innate Immune System
R-HSA-937061 TRIF-mediated TLR3/TLR4 signaling
R-HSA-168179 Toll Like Receptor TLR1:TLR2 Cascade
R-HSA-168138 Toll Like Receptor 9 (TLR9) Cascade
R-HSA-181438 Toll Like Receptor 2 (TLR2) Cascade
R-HSA-166016 Toll Like Receptor 4 (TLR4) Cascade
R-HSA-168188 Toll Like Receptor TLR6:TLR2 Cascade
R-HSA-933542 TRAF6 mediated NF-kB activation
R-HSA-1606322 ZBP1(DAI) mediated induction of type I IFNs
R-HSA-166058 MyD88:Mal cascade initiated on plasma membrane
R-HSA-168181 Toll Like Receptor 7/8 (TLR7/8) Cascade
R-HSA-975155 MyD88 dependent cascade initiated on endosome
R-HSA-168928 RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways
R-HSA-2454202 Fc epsilon receptor (FCERI) signaling
R-HSA-166054 Activated TLR4 signalling
R-HSA-1834949 Cytosolic sensors of pathogen-associated DNA
R-HSA-2871837 FCERI mediated NF-kB activation
R-HSA-5621481 C-type lectin receptors (CLRs)
R-HSA-1810476 RIP-mediated NFkB activation via ZBP1
R-HSA-5607764 CLEC7A (Dectin-1) signaling
R-HSA-162582 Signal Transduction
R-HSA-166520 Signalling by NGF
R-HSA-193704 p75 NTR receptor-mediated signalling
R-HSA-209560 NF-kB is activated and signals survival
R-HSA-193639 p75NTR signals via NF-kB
Pathway Interaction Database
aurora_a_pathway Aurora A signaling
lysophospholipid_pathway LPA receptor mediated events
avb3_opn_pathway Osteopontin-mediated events
hivnefpathway HIV-1 Nef: Negative effector of Fas and TNF-alpha
hdac_classi_pathway Signaling events mediated by HDAC Class I
ceramide_pathway Ceramide signaling pathway
nfkappabcanonicalpathway Canonical NF-kappaB pathway
bcr_5pathway BCR signaling pathway
il23pathway IL23-mediated signaling events
nfkappabatypicalpathway Atypical NF-kappaB pathway
cd40_pathway CD40/CD40L signaling
WikiPathways
WP127 IL-5 Signaling Pathway
WP1433 NOD pathway
WP1772 Apoptosis Modulation and Signaling
WP254 Apoptosis
WP231 TNF-alpha/NF-kB Signaling Pathway
WP61 Delta-Notch Signaling Pathway
WP75 Toll-like receptor signaling pathway
WP69 TCR Signaling Pathway
WP23 B Cell Receptor Signaling Pathway
WP2377 Integrated Pancreatic Cancer Pathway
WP2374 Oncostatin M Signaling Pathway
WP2380 BDNF signaling pathway
WP2018 RANKL/RANK Signaling Pathway
WP2036 TWEAK Signaling Pathway
WP2037 Prolactin Signaling Pathway
WP2203 TSLP Signaling Pathway
WP2324 AGE/RAGE pathway
WP1449 Regulation of toll-like receptor signaling pathway
WP2355 Corticotropin-releasing hormone


Homo sapiens (human) NFKBIA NP_065390.1
Pan troglodytes (chimpanzee) NFKBIA XP_522823.2
Macaca mulatta (Rhesus monkey) NFKBIA XP_001087842.1
Canis lupus familiaris (dog) NFKBIA XP_537413.3
Bos taurus (cattle) NFKBIA NP_001039333.1
Mus musculus (house mouse) Nfkbia NP_035037.2
Rattus norvegicus (Norway rat) Nfkbia NP_001099190.2
Gallus gallus (chicken) NFKBIA NP_001001472.2
Danio rerio (zebrafish) nfkbiab NP_955923.1
Danio rerio (zebrafish) nfkbiaa NP_998349.1
Drosophila melanogaster (fruit fly) cact NP_001260496.1
Xenopus (Silurana) tropicalis (western clawed frog) nfkbia NP_001004851.1

Component

ID Name Evidence
GO:0005634 nucleus IDA
GO:0005737 cytoplasm IDA
GO:0005829 cytosol EXP
GO:0005829 cytosol TAS
GO:0005886 plasma membrane IDA
GO:0033256 I-kappaB/NF-kappaB complex TAS

Function

ID Name Evidence
GO:0005515 protein binding IPI
GO:0008134 transcription factor binding TAS
GO:0008139 nuclear localization sequence binding IPI
GO:0019899 enzyme binding IPI
GO:0031072 heat shock protein binding IEA
GO:0031625 ubiquitin protein ligase binding IPI
GO:0032403 protein complex binding IEA
GO:0042802 identical protein binding IPI
GO:0051059 NF-kappaB binding IDA
GO:0051059 NF-kappaB binding IPI

Process

ID Name Evidence
GO:0000060 protein import into nucleus, translocation IEA
GO:0002224 toll-like receptor signaling pathway TAS
GO:0002755 MyD88-dependent toll-like receptor signaling pathway EXP
GO:0002755 MyD88-dependent toll-like receptor signaling pathway TAS
GO:0002756 MyD88-independent toll-like receptor signaling pathway EXP
GO:0002756 MyD88-independent toll-like receptor signaling pathway TAS
GO:0006915 apoptosis TAS
GO:0006916 anti-apoptosis EXP
GO:0007253 cytoplasmic sequestering of NF-kappaB IMP
GO:0008063 Toll signaling pathway TAS
GO:0010745 negative regulation of macrophage derived foam cell differentiation IMP
GO:0010875 positive regulation of cholesterol efflux IMP
GO:0010888 negative regulation of lipid storage IMP
GO:0031663 lipopolysaccharide-mediated signaling pathway IEA
GO:0032088 negative regulation of NF-kappaB transcription factor activity IDA
GO:0032270 positive regulation of cellular protein metabolic process IMP
GO:0032495 response to muramyl dipeptide IEA
GO:0032496 response to lipopolysaccharide IEA
GO:0034130 toll-like receptor 1 signaling pathway TAS
GO:0034134 toll-like receptor 2 signaling pathway TAS
GO:0034138 toll-like receptor 3 signaling pathway TAS
GO:0034142 toll-like receptor 4 signaling pathway TAS
GO:0042127 regulation of cell proliferation IEA
GO:0042345 regulation of NF-kappaB import into nucleus NAS
GO:0042994 cytoplasmic sequestering of transcription factor IDA
GO:0043330 response to exogenous dsRNA IEA
GO:0043392 negative regulation of DNA binding NAS
GO:0044419 interspecies interaction between organisms IEA
GO:0045087 innate immune response TAS
GO:0045638 negative regulation of myeloid cell differentiation IEA
GO:0045746 negative regulation of Notch signaling pathway IEA
GO:0045944 positive regulation of transcription from RNA polymerase II promoter IMP
GO:0048011 nerve growth factor receptor signaling pathway TAS
GO:0050852 T cell receptor signaling pathway TAS
GO:0051092 positive regulation of NF-kappaB transcription factor activity TAS
GO:0070417 cellular response to cold NAS
GO:0070427 nucleotide-binding oligomerization domain containing 1 signaling pathway IEA
GO:0070431 nucleotide-binding oligomerization domain containing 2 signaling pathway IEA

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NFKBIA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFKBIA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19341
NM_020529 Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OHu19341
Accession Version NM_020529.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product NF-kappa-B inhibitor alpha
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL133163.3, BC004983.1 and BU737609.1. This sequence is a reference standard in the RefSeqGene project. On Feb 23, 2008 this sequence version replaced gi:10092618. Summary: This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002601.2, M69043.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTTCCAGG CGGCCGAGCG CCCCCAGGAG TGGGCCATGG AGGGCCCCCG CGACGGGCTG 
AAGAAGGAGC GGCTACTGGA CGACCGCCAC GACAGCGGCC TGGACTCCAT GAAAGACGAG
GAGTACGAGC AGATGGTCAA GGAGCTGCAG GAGATCCGCC TCGAGCCGCA GGAGGTGCCG
CGCGGCTCGG AGCCCTGGAA GCAGCAGCTC ACCGAGGACG GGGACTCGTT CCTGCACTTG
GCCATCATCC ATGAAGAAAA GGCACTGACC ATGGAAGTGA TCCGCCAGGT GAAGGGAGAC
CTGGCCTTCC TCAACTTCCA GAACAACCTG CAGCAGACTC CACTCCACTT GGCTGTGATC
ACCAACCAGC CAGAAATTGC TGAGGCACTT CTGGGAGCTG GCTGTGATCC TGAGCTCCGA
GACTTTCGAG GAAATACCCC CCTACACCTT GCCTGTGAGC AGGGCTGCCT GGCCAGCGTG
GGAGTCCTGA CTCAGTCCTG CACCACCCCG CACCTCCACT CCATCCTGAA GGCTACCAAC
TACAATGGCC ACACGTGTCT ACACTTAGCC TCTATCCATG GCTACCTGGG CATCGTGGAG
CTTTTGGTGT CCTTGGGTGC TGATGTCAAT GCTCAGGAGC CCTGTAATGG CCGGACTGCC
CTTCACCTCG CAGTGGACCT GCAAAATCCT GACCTGGTGT CACTCCTGTT GAAGTGTGGG
GCTGATGTCA ACAGAGTTAC CTACCAGGGC TATTCTCCCT ACCAGCTCAC CTGGGGCCGC
CCAAGCACCC GGATACAGCA GCAGCTGGGC CAGCTGACAC TAGAAAACCT TCAGATGCTG
CCAGAGAGTG AGGATGAGGA GAGCTATGAC ACAGAGTCAG AGTTCACGGA GTTCACAGAG
GACGAGCTGC CCTATGATGA CTGTGTGTTT GGAGGCCAGC GTCTGACGTT ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_065390.1
CDS111..1064
Misc Feature(1)171..173(+)
Misc Feature(2)171..173(+)
Misc Feature(3)174..176(+)
Misc Feature(4)198..218(+)
Misc Feature(5)204..206(+)
Misc Feature(6)204..206(+)
Misc Feature(7)204..206(+)
Misc Feature(8)204..206(+)
Misc Feature(9)204..206(+)
Misc Feature(10)204..206(+)
Misc Feature(11)216..218(+)
Misc Feature(12)216..218(+)
Misc Feature(13)216..218(+)
Misc Feature(14)216..218(+)
Misc Feature(15)216..218(+)
Misc Feature(16)234..236(+)
Misc Feature(17)234..236(+)
Misc Feature(18)234..236(+)
Misc Feature(19)234..236(+)
Misc Feature(20)243..272(+)
Misc Feature(21)327..419(+)
Misc Feature(22)330..503(+)
Misc Feature(23)423..821(+)
Misc Feature(24)438..527(+)
Misc Feature(25)438..470(+)
Misc Feature(26)537..626(+)
Misc Feature(27)552..848(+)
Misc Feature(28)654..743(+)
Misc Feature(29)738..740(+)
Misc Feature(30)756..845(+)
Misc Feature(31)840..842(+)
Misc Feature(32)957..959(+)
Misc Feature(33)957..959(+)
Misc Feature(34)972..974(+)
Misc Feature(35)972..974(+)
Misc Feature(36)981..983(+)
Misc Feature(37)981..983(+)
Misc Feature(38)987..989(+)
Misc Feature(39)987..989(+)
Misc Feature(40)1005..1007(+)
Exon (1)1..337
Gene:NFKBIA
Gene Synonym:
Exon (2)338..446
Gene:NFKBIA
Gene Synonym:
Exon (3)447..657
Gene:NFKBIA
Gene Synonym:
Exon (4)658..746
Gene:NFKBIA
Gene Synonym:
Exon (5)747..1016
Gene:NFKBIA
Gene Synonym:
Exon (6)1017..1567
Gene:NFKBIA
Gene Synonym:
Translation
Position Chain Variation Link
3 3 c, g dbSNP:570469596
20 20 c, t dbSNP:550686147
25 25 c, g, t dbSNP:11569615
31 31 -, gtcccgcc dbSNP:540103354
34 34 -, ccgcccgc dbSNP:11569617
35 35 c, t dbSNP:531808763
45 45 a, g, t dbSNP:2273651
46 46 c, t dbSNP:543302234
47 47 a, g dbSNP:529614549
49 49 a, g dbSNP:368072323
50 50 a, g dbSNP:560482972
64 64 c, t dbSNP:779679632
70 70 a, g dbSNP:540282095
77 77 a, g dbSNP:2233410
78 78 a, c dbSNP:577994340
79 79 a, g dbSNP:749968901
80 80 c, g dbSNP:765624161
82 82 c, t dbSNP:757788159
90 90 c, t dbSNP:557951647
94 94 c, t dbSNP:754145057
99 99 c, t dbSNP:764501009
101 101 c, t dbSNP:760886864
102 102 c, g dbSNP:775733297
105 105 a, c, t dbSNP:200864499
108 108 a, g dbSNP:759488129
110 110 a, c dbSNP:774490774
122 122 a, g dbSNP:769557443
137 137 a, g dbSNP:748171915
142 142 a, g dbSNP:121913664
146 146 a, c dbSNP:776559937
150 150 g, t dbSNP:121913665
154 154 a, g dbSNP:768610997
162 162 a, g dbSNP:746636446
173 173 a, g dbSNP:779802553
174 174 -, aag dbSNP:760942646
176 176 a, g dbSNP:575087658
184 184 c, t dbSNP:758102701
186 186 a, c dbSNP:200033269
188 188 a, g dbSNP:8192285
191 191 c, t dbSNP:1957106
196 196 g, t dbSNP:754412949
205 205 g, t dbSNP:28933100
221 221 a, g dbSNP:764415243
222 222 a, c dbSNP:373643552
227 227 c, g dbSNP:753085459
230 230 a, g dbSNP:767565601
236 236 c, t dbSNP:759815989
261 261 a, g dbSNP:774277544
266 266 a, c dbSNP:369801548
268 268 a, g dbSNP:761810172
272 272 c, t dbSNP:138468321
279 279 c, t dbSNP:768735436
289 289 c, t dbSNP:371482940
293 293 c, t dbSNP:775432076
294 294 a, g dbSNP:771568277
296 296 c, t dbSNP:143798499
298 298 c, g, t dbSNP:376762724
302 302 a, g dbSNP:552899761
303 303 c, g dbSNP:778206898
304 304 c, t dbSNP:756648390
305 305 c, t dbSNP:752999258
316 316 a, t dbSNP:781662093
318 318 c, g dbSNP:755146648
329 329 c, t dbSNP:371348054
330 330 c, g dbSNP:766345843
331 331 c, g dbSNP:763076696
332 332 a, g, t dbSNP:764058016
335 335 c, t dbSNP:760704249
338 338 a, g dbSNP:772702898
342 342 c, t dbSNP:745443930
347 347 c, t dbSNP:769330755
353 353 c, t dbSNP:2233412
372 372 a, g dbSNP:556918000
375 375 c, t dbSNP:201058640
380 380 a, c, t dbSNP:780297815
381 381 a, c dbSNP:758681852
392 392 c, t dbSNP:2233413
393 393 c, t dbSNP:779105881
407 407 a, c dbSNP:757465465
409 409 a, g dbSNP:753891384
411 411 a, c dbSNP:767452313
416 416 c, t dbSNP:1050851
420 420 c, t dbSNP:751353414
425 425 c, t dbSNP:766226600
431 431 c, g dbSNP:762557295
435 435 a, c dbSNP:772897174
436 436 a, g dbSNP:150221265
437 437 c, t dbSNP:761346881
438 438 c, t dbSNP:776007975
447 447 a, g dbSNP:574509775
450 450 c, t dbSNP:763639524
451 451 c, t dbSNP:760056320
454 454 a, t dbSNP:752288851
475 475 a, g dbSNP:768072084
478 478 a, g dbSNP:142422402
488 488 g, t dbSNP:774847049
489 489 a, g dbSNP:377380656
496 496 c, t dbSNP:763208600
501 501 c, t dbSNP:773256852
505 505 g, t dbSNP:554698790
506 506 a, t dbSNP:534752743
519 519 c, t dbSNP:147627228
529 529 a, g dbSNP:771984394
537 537 a, c, g dbSNP:778739283
538 538 a, g dbSNP:757053022
547 547 c, t dbSNP:753691609
548 548 c, t dbSNP:777254521
551 551 a, c, g, t dbSNP:375015401
552 552 c, t dbSNP:371253544
558 558 c, t dbSNP:377594611
560 560 c, t dbSNP:752157617
561 561 g, t dbSNP:766929321
575 575 c, t dbSNP:763183892
580 580 c, t dbSNP:372930764
587 587 a, c, t dbSNP:199877737
596 596 c, g dbSNP:762080279
611 611 c, t dbSNP:200219960
614 614 c, t dbSNP:768764676
615 615 a, g dbSNP:745783613
616 616 c, t dbSNP:151228045
619 619 c, t dbSNP:770933211
620 620 a, g dbSNP:748972316
635 635 c, g dbSNP:777640654
636 636 c, g dbSNP:755819611
641 641 a, g dbSNP:747880612
645 645 a, g dbSNP:780823092
649 649 a, c dbSNP:376763952
653 653 c, t dbSNP:751108447
659 659 c, t dbSNP:769491611
663 663 a, g dbSNP:760968064
664 664 c, t dbSNP:142195196
672 672 c, t dbSNP:767707931
679 679 c, t dbSNP:762771956
686 686 c, t dbSNP:773149235
691 691 c, g dbSNP:148656104
693 693 a, t dbSNP:761632092
694 694 a, g dbSNP:780815318
696 696 c, t dbSNP:768407309
704 704 c, t dbSNP:746741230
705 705 g, t dbSNP:779399614
717 717 a, g dbSNP:771660743
721 721 c, t dbSNP:745392200
723 723 c, t dbSNP:779353997
727 727 a, g dbSNP:757768073
734 734 c, t dbSNP:754147035
737 737 c, t dbSNP:778172206
740 740 c, t dbSNP:756290170
751 751 c, t dbSNP:373228608
770 770 c, g dbSNP:781404120
779 779 a, c, t dbSNP:143869405
801 801 g, t dbSNP:766631284
803 803 c, t dbSNP:757228861
804 804 c, t dbSNP:753873999
809 809 a, g dbSNP:763960349
815 815 c, t dbSNP:760478586
830 830 a, g dbSNP:369802093
839 839 c, t dbSNP:767141805
849 849 a, g dbSNP:759246777
853 853 a, g dbSNP:773849463
858 858 c, g dbSNP:770472070
860 860 c, t dbSNP:749777504
863 863 c, t dbSNP:773665307
881 881 c, t dbSNP:770202858
888 888 c, t dbSNP:528542318
889 889 g, t dbSNP:781464425
893 893 a, c dbSNP:376370831
895 895 a, g dbSNP:755221348
900 900 c, t dbSNP:747273949
901 901 a, g dbSNP:149917774
911 911 g, t dbSNP:758502941
932 932 a, g dbSNP:750562815
942 942 a, c dbSNP:373499073
951 951 c, t dbSNP:756020078
953 953 a, g dbSNP:752524885
954 954 a, g dbSNP:767285341
958 958 a, g dbSNP:759161424
972 972 a, g dbSNP:774043011
982 982 c, g dbSNP:766046360
984 984 c, g dbSNP:762379232
986 986 a, g dbSNP:772607545
997 997 c, t dbSNP:201191831
998 998 a, g dbSNP:139180460
1004 1004 c, t dbSNP:777020702
1006 1006 c, t dbSNP:769191348
1013 1013 c, t dbSNP:146132589
1014 1014 a, g dbSNP:116801398
1020 1020 c, t dbSNP:751338755
1021 1021 c, g dbSNP:779867290
1022 1022 c, t dbSNP:758044062
1024 1024 a, g dbSNP:750102278
1039 1039 c, t dbSNP:1803939
1041 1041 a, g dbSNP:764958121
1044 1044 -, c dbSNP:34378588
1049 1049 g, t dbSNP:761161778
1050 1050 c, t dbSNP:753419932
1051 1051 a, g dbSNP:142970414
1054 1054 g, t dbSNP:761313156
1057 1057 c, t dbSNP:775963900
1058 1058 a, g dbSNP:772630322
1066 1066 c, t dbSNP:8904
1067 1067 a, g dbSNP:368317245
1068 1068 c, t dbSNP:375907017
1070 1070 a, g dbSNP:749282975
1073 1073 a, g dbSNP:777883239
1078 1078 a, g dbSNP:768552445
1082 1082 c, g dbSNP:9333352
1085 1085 a, c dbSNP:779814000
1088 1088 a, g dbSNP:375256332
1093 1093 -, t dbSNP:760073242
1095 1095 c, t dbSNP:752005120
1105 1105 a, c dbSNP:569822955
1106 1106 a, c dbSNP:778389686
1108 1108 a, c, g dbSNP:753264298
1111 1111 -, aaaa dbSNP:774814890
1111 1111 a, g dbSNP:763736452
1114 1114 -, a dbSNP:770482283
1114 1114 -, a dbSNP:748787798
1114 1114 a, g dbSNP:756683575
1118 1118 c, t dbSNP:191372109
1135 1135 a, g dbSNP:1131420
1138 1138 a, g dbSNP:138781182
1140 1140 -, aag dbSNP:370364535
1140 1140 -, aaa dbSNP:200984079
1142 1142 -, gaagaaaaaa dbSNP:530975586
1142 1142 a, g dbSNP:149783842
1145 1145 -, gaa dbSNP:145753299
1145 1145 g, t dbSNP:112369437
1151 1151 a, t dbSNP:3209769
1163 1163 a, t dbSNP:529984279
1172 1172 c, g dbSNP:147363386
1173 1173 c, t dbSNP:142696288
1175 1175 c, t dbSNP:540995241
1176 1176 a, g dbSNP:762277256
1188 1188 c, t dbSNP:570132393
1190 1190 a, g dbSNP:696
1194 1194 c, t dbSNP:564770241
1199 1199 c, t dbSNP:545040308
1201 1201 c, t dbSNP:575753227
1207 1207 -, aa, tt dbSNP:11569610
1222 1222 a, c dbSNP:187988391
1240 1240 c, g dbSNP:45438895
1248 1248 g, t dbSNP:543593582
1256 1256 a, g dbSNP:574717633
1259 1259 c, t dbSNP:182929073
1289 1289 c, t dbSNP:535046409
1327 1327 g, t dbSNP:11569620
1341 1341 a, c dbSNP:373095812
1367 1367 c, t dbSNP:566538132
1376 1376 a, g dbSNP:539095991
1400 1400 g, t dbSNP:113277534
1430 1430 a, g dbSNP:552418731
1448 1448 a, g dbSNP:570182384
1485 1485 a, g dbSNP:2273650
1490 1490 c, t dbSNP:536430863
1497 1497 c, t dbSNP:116765051
1498 1498 a, t dbSNP:747730828
1552 1552 a, g dbSNP:776503882
1556 1556 a, t dbSNP:547662178

Target ORF information:

RefSeq Version NM_020529
Organism Homo sapiens (human)
Definition Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_020529

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTTCCAGG CGGCCGAGCG CCCCCAGGAG TGGGCCATGG AGGGCCCCCG CGACGGGCTG 
AAGAAGGAGC GGCTACTGGA CGACCGCCAC GACAGCGGCC TGGACTCCAT GAAAGACGAG
GAGTACGAGC AGATGGTCAA GGAGCTGCAG GAGATCCGCC TCGAGCCGCA GGAGGTGCCG
CGCGGCTCGG AGCCCTGGAA GCAGCAGCTC ACCGAGGACG GGGACTCGTT CCTGCACTTG
GCCATCATCC ATGAAGAAAA GGCACTGACC ATGGAAGTGA TCCGCCAGGT GAAGGGAGAC
CTGGCCTTCC TCAACTTCCA GAACAACCTG CAGCAGACTC CACTCCACTT GGCTGTGATC
ACCAACCAGC CAGAAATTGC TGAGGCACTT CTGGGAGCTG GCTGTGATCC TGAGCTCCGA
GACTTTCGAG GAAATACCCC CCTACACCTT GCCTGTGAGC AGGGCTGCCT GGCCAGCGTG
GGAGTCCTGA CTCAGTCCTG CACCACCCCG CACCTCCACT CCATCCTGAA GGCTACCAAC
TACAATGGCC ACACGTGTCT ACACTTAGCC TCTATCCATG GCTACCTGGG CATCGTGGAG
CTTTTGGTGT CCTTGGGTGC TGATGTCAAT GCTCAGGAGC CCTGTAATGG CCGGACTGCC
CTTCACCTCG CAGTGGACCT GCAAAATCCT GACCTGGTGT CACTCCTGTT GAAGTGTGGG
GCTGATGTCA ACAGAGTTAC CTACCAGGGC TATTCTCCCT ACCAGCTCAC CTGGGGCCGC
CCAAGCACCC GGATACAGCA GCAGCTGGGC CAGCTGACAC TAGAAAACCT TCAGATGCTG
CCAGAGAGTG AGGATGAGGA GAGCTATGAC ACAGAGTCAG AGTTCACGGA GTTCACAGAG
GACGAGCTGC CCTATGATGA CTGTGTGTTT GGAGGCCAGC GTCTGACGTT ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Ribosomal protein S3 interacts with the NF-kappaB inhibitor IkappaBalpha
FEBS Lett. 588 (5), 659-664 (2014)
Stanborough T, Niederhauser J, Koch B, Bergler H and Pertschy B.


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The p97-UFD1L-NPL4 protein complex mediates cytokine-induced IkappaBalpha proteolysis
Mol. Cell. Biol. 34 (3), 335-347 (2014)
Li JM, Wu H, Zhang W, Blackburn MR and Jin J.


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Direct observation of a transient ternary complex during IkappaBalpha-mediated dissociation of NF-kappaB from DNA
Proc. Natl. Acad. Sci. U.S.A. 111 (1), 225-230 (2014)
Alverdi V, Hetrick B, Joseph S and Komives EA.


book

Long-term incubation with proteasome inhibitors (PIs) induces IkappaBalpha degradation via the lysosomal pathway in an IkappaB kinase (IKK)-dependent and IKK-independent manner
J. Biol. Chem. 288 (45), 32777-32786 (2013)
Lee KH, Jeong J and Yoo CG.


book

Intracellular NF-kB-decrease and IKBalpha increase in human macrophages following CTLA4-Ig treatment
Clin. Exp. Rheumatol. 31 (6), 943-946 (2013)
Cutolo M, Soldano S, Contini P, Sulli A, Seriolo B, Montagna P and Brizzolara R.


book

Signal-induced degradation of I kappa B alpha requires site-specific ubiquitination
Proc. Natl. Acad. Sci. U.S.A. 92 (24), 11259-11263 (1995)
Scherer DC, Brockman JA, Chen Z, Maniatis T and Ballard DW.


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I kappa B/MAD-3 masks the nuclear localization signal of NF-kappa B p65 and requires the transactivation domain to inhibit NF-kappa B p65 DNA binding
Mol. Biol. Cell 3 (12), 1339-1352 (1992)
Ganchi PA, Sun SC, Greene WC and Ballard DW.


book

Chromosomal localization of the genes encoding the p50/p105 subunits of NF-kappa B (NFKB2) and the I kappa B/MAD-3 (NFKBI) inhibitor of NF-kappa B to 4q24 and 14q13, respectively
Genomics 14 (2), 529-531 (1992)
Le Beau MM, Ito C, Cogswell P, Espinosa R 3rd, Fernald AA and Baldwin AS Jr.


book

Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity
Cell 65 (7), 1281-1289 (1991)
Haskill S, Beg AA, Tompkins SM, Morris JS, Yurochko AD, Sampson-Johannes A, Mondal K, Ralph P and Baldwin AS Jr.


book

I kappa B: a specific inhibitor of the NF-kappa B transcription factor
Science 242 (4878), 540-546 (1988)
Baeuerle PA and Baltimore D.


 
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