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NGF cDNA ORF clone, Homo sapiens (human)

Gene Symbol NGF
Entrez Gene ID 4803
Full Name nerve growth factor (beta polypeptide)
Synonyms Beta-NGF, HSAN5, NGFB
General protein information
Preferred Names
beta-nerve growth factor
Names
beta-nerve growth factor
nerve growth factor, beta subunit
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p13.1

Summary This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

162030

Disorder Html: Neuropathy, hereditary sensory and autonomic, type V, 608654 (3)





The following NGF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NGF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu59274 XM_011541518 PREDICTED: Homo sapiens nerve growth factor (beta polypeptide) (NGF), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
OHu25822 XM_006710663 PREDICTED: Homo sapiens nerve growth factor (beta polypeptide) (NGF), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$139.30
$199.00
OHu25822 XM_006710665 PREDICTED: Homo sapiens nerve growth factor (beta polypeptide) (NGF), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$139.30
$199.00
OHu25822 NM_002506 Homo sapiens nerve growth factor (beta polypeptide) (NGF), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$139.30
$199.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu59274
Accession Version XM_011541518.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 891bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product beta-nerve growth factor isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_032977.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCCTCAT CTAATGGACA CTTTAATGAA GTTTTGGCCA GTGGTCGTGC AGTCCAAGGG 
GCTGGATGGC ATGCTGGACC CAAGCTCAGC TCAGCGTCCG GACCCAATAA CAGTTTTACC
AAGGGAGCAG CTTTCTATCC TGGCCACACT GAGGTGCATA GCGTAATGTC CATGTTGTTC
TACACTCTGA TCACAGCTTT TCTGATCGGC ATACAGGCGG AACCACACTC AGAGAGCAAT
GTCCCTGCAG GACACACCAT CCCCCAAGCC CACTGGACTA AACTTCAGCA TTCCCTTGAC
ACTGCCCTTC GCAGAGCCCG CAGCGCCCCG GCAGCGGCGA TAGCTGCACG CGTGGCGGGG
CAGACCCGCA ACATTACTGT GGACCCCAGG CTGTTTAAAA AGCGGCGACT CCGTTCACCC
CGTGTGCTGT TTAGCACCCA GCCTCCCCGT GAAGCTGCAG ACACTCAGGA TCTGGACTTC
GAGGTCGGTG GTGCTGCCCC CTTCAACAGG ACTCACAGGA GCAAGCGGTC ATCATCCCAT
CCCATCTTCC ACAGGGGCGA ATTCTCGGTG TGTGACAGTG TCAGCGTGTG GGTTGGGGAT
AAGACCACCG CCACAGACAT CAAGGGCAAG GAGGTGATGG TGTTGGGAGA GGTGAACATT
AACAACAGTG TATTCAAACA GTACTTTTTT GAGACCAAGT GCCGGGACCC AAATCCCGTT
GACAGCGGGT GCCGGGGCAT TGACTCAAAG CACTGGAACT CATATTGTAC CACGACTCAC
ACCTTTGTCA AGGCGCTGAC CATGGATGGC AAGCAGGCTG CCTGGCGGTT TATCCGGATA
GATACGGCCT GTGTGTGTGT GCTCAGCAGG AAGGCTGTGA GAAGAGCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011539820.1
CDS220..1110
Misc Feature(1)757..1101(+)
Translation
Position Chain Variation Link
19 19 a, g dbSNP:565459268
23 23 a, g dbSNP:767434906
78 78 a, g dbSNP:546941788
92 92 c, t dbSNP:144295125
101 101 c, t dbSNP:111684725
108 108 c, t dbSNP:72991986
128 128 c, t dbSNP:765102016
144 144 a, g dbSNP:761645016
162 162 a, g dbSNP:547372967
166 166 a, g dbSNP:531262596
167 167 c, t dbSNP:776376861
178 178 c, t dbSNP:764083625
179 179 a, g dbSNP:4573519
187 187 c, t dbSNP:775081224
232 232 a, g dbSNP:182452565
234 234 c, t dbSNP:771416966
240 240 c, t dbSNP:189603548
256 256 a, g dbSNP:747462818
289 289 a, c dbSNP:61730971
314 314 c, t dbSNP:756375071
315 315 a, g dbSNP:767567207
318 318 c, t dbSNP:535930103
319 319 a, g dbSNP:780222620
363 363 a, c dbSNP:572125330
378 378 c, t dbSNP:376149287
380 380 a, g dbSNP:745683574
381 381 c, g dbSNP:781065610
382 382 a, g, t dbSNP:750055928
386 386 c, t dbSNP:751638085
395 395 c, t dbSNP:777773454
402 402 c, t dbSNP:370169329
426 426 c, t dbSNP:752800027
427 427 a, c, g dbSNP:181255687
437 437 c, t dbSNP:754287903
438 438 a, g dbSNP:6325
439 439 a, g dbSNP:761319558
443 443 c, t dbSNP:773903815
453 453 a, g, t dbSNP:762536454
462 462 c, t dbSNP:150188752
467 467 c, t dbSNP:769465872
474 474 c, t dbSNP:745630230
488 488 c, t dbSNP:6330
490 490 c, t dbSNP:267597943
493 493 c, t dbSNP:770843971
502 502 c, g dbSNP:748028952
509 509 a, c dbSNP:746985577
512 512 c, t dbSNP:566033399
523 523 g, t dbSNP:185385214
524 524 a, c, t dbSNP:181709715
526 526 c, t dbSNP:779240755
529 529 c, t dbSNP:146716262
530 530 a, g dbSNP:781072056
534 534 a, g dbSNP:754238855
538 538 c, t dbSNP:766912679
539 539 a, g, t dbSNP:149054069
540 540 a, c dbSNP:750836533
543 543 c, t dbSNP:376241395
544 544 -, gccccgg dbSNP:768548505
544 544 a, g dbSNP:200459956
548 548 c, t dbSNP:775019570
549 549 a, c, g dbSNP:149876217
552 552 a, t dbSNP:776406021
554 554 c, t dbSNP:770647680
555 555 a, g dbSNP:746837424
557 557 c, t dbSNP:201861727
558 558 a, g dbSNP:147326889
560 560 c, t dbSNP:748148537
564 564 c, t dbSNP:779189568
568 568 c, t dbSNP:755243469
569 569 a, g dbSNP:369266810
570 570 c, t dbSNP:780432648
571 571 a, c, g dbSNP:750829893
572 572 c, t dbSNP:767878560
573 573 a, g dbSNP:757830779
575 575 a, c, t dbSNP:201087374
576 576 a, g dbSNP:759051358
577 577 a, g dbSNP:776208737
580 580 a, c dbSNP:766082334
585 585 c, g dbSNP:760350868
586 586 c, g, t dbSNP:572066909
587 587 a, g dbSNP:377253859
591 591 c, t dbSNP:372994894
592 592 a, g dbSNP:768794852
598 598 a, g dbSNP:11466110
603 603 c, t dbSNP:780383333
606 606 c, t dbSNP:756396299
608 608 a, g dbSNP:141486298
609 609 c, g, t dbSNP:757701792
613 613 c, t dbSNP:751997242
622 622 c, t dbSNP:778266438
623 623 a, g dbSNP:11466111
625 625 a, c dbSNP:753382007
627 627 a, g dbSNP:766849758
630 630 c, t dbSNP:765918245
631 631 c, t dbSNP:138175552
632 632 a, c, g dbSNP:767272925
635 635 c, t dbSNP:267597942
636 636 a, t dbSNP:761709969
640 640 c, t dbSNP:774379026
641 641 a, g, t dbSNP:200629339
646 646 c, t dbSNP:775686730
648 648 a, g dbSNP:769893063
649 649 c, t dbSNP:746169118
660 660 a, g dbSNP:202060337
667 667 c, t dbSNP:771270154
668 668 a, g dbSNP:150136942
676 676 a, g dbSNP:537372354
682 682 a, c dbSNP:758948909
683 683 c, t dbSNP:142538674
693 693 g, t dbSNP:372694067
699 699 c, t dbSNP:779678303
700 700 a, g dbSNP:755580704
702 702 a, g dbSNP:750030065
705 705 c, t dbSNP:202005277
706 706 a, g dbSNP:145894043
716 716 c, t dbSNP:751440257
719 719 a, c, g dbSNP:147763877
720 720 c, t dbSNP:35941329
726 726 c, t dbSNP:775429651
727 727 a, g dbSNP:376618971
747 747 a, g dbSNP:759547617
754 754 a, t dbSNP:145691700
763 763 a, c dbSNP:28758211
765 765 c, t dbSNP:372985633
771 771 c, t dbSNP:747348126
777 777 c, t dbSNP:778162180
778 778 a, g dbSNP:772557857
783 783 a, c dbSNP:746897874
786 786 a, g dbSNP:565841831
788 788 c, t dbSNP:755599709
801 801 c, t dbSNP:369555032
803 803 a, g dbSNP:201867223
804 804 a, c, t dbSNP:751386981
805 805 a, c, g dbSNP:199511298
812 812 a, t dbSNP:758273427
821 821 a, g dbSNP:752622800
823 823 -, aacc dbSNP:370724679
825 825 c, t dbSNP:765111580
826 826 a, c dbSNP:202138909
828 828 c, t dbSNP:759622419
829 829 a, g dbSNP:149823633
832 832 a, g dbSNP:766568424
850 850 a, g dbSNP:369927492
853 853 a, g dbSNP:773565185
866 866 c, g dbSNP:139830389
867 867 a, g dbSNP:748481624
871 871 a, g dbSNP:774878867
882 882 a, c dbSNP:769082615
888 888 c, t dbSNP:745359909
892 892 c, t dbSNP:139541754
896 896 a, g dbSNP:375808064
898 898 c, g dbSNP:746593757
914 914 c, t dbSNP:777582167
923 923 a, g dbSNP:758166016
936 936 c, t dbSNP:532714783
937 937 a, g dbSNP:141889164
940 940 a, g dbSNP:201152024
945 945 c, t dbSNP:779744960
946 946 a, c, g dbSNP:760753923
948 948 c, g dbSNP:773369123
976 976 a, g dbSNP:767755905
981 981 a, t dbSNP:762203206
984 984 c, t dbSNP:774626005
992 992 c, t dbSNP:769190598
993 993 a, c, g dbSNP:143157422
1001 1001 c, t dbSNP:770508381
1014 1014 a, g dbSNP:112292538
1019 1019 c, g dbSNP:777529073
1029 1029 c, t dbSNP:371529651
1040 1040 c, g dbSNP:371216746
1045 1045 c, t dbSNP:11466112
1051 1051 a, t dbSNP:80277627
1061 1061 a, g dbSNP:34542615
1064 1064 -, tagatac dbSNP:746752363
1065 1065 a, c, g dbSNP:565497625
1068 1068 c, t dbSNP:549646930
1071 1071 c, t dbSNP:750539380
1073 1073 c, t dbSNP:767703003
1079 1079 -, tg dbSNP:142696332
1080 1080 a, g dbSNP:761997516
1083 1083 c, g dbSNP:531522066
1090 1090 a, c dbSNP:561107153
1093 1093 g, t dbSNP:763390069
1098 1098 a, g dbSNP:776092330
1107 1107 c, t dbSNP:34079702
1110 1110 a, c dbSNP:770457149
1111 1111 c, g dbSNP:760233445
1112 1112 c, t dbSNP:772900409
1116 1116 a, c, g, t dbSNP:542460046
1117 1117 a, g dbSNP:768283373
1119 1119 c, t dbSNP:749107814
1121 1121 c, g, t dbSNP:199812295
1122 1122 a, g dbSNP:560280132
1123 1123 c, t dbSNP:545171466
1128 1128 -, t dbSNP:758257834
1132 1132 c, g dbSNP:757362434
1144 1144 a, g dbSNP:751673509
1151 1151 a, c, t dbSNP:763370576
1185 1185 c, t dbSNP:757967985
1240 1240 c, t dbSNP:749879805

Target ORF information:

RefSeq Version XM_011541518
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens nerve growth factor (beta polypeptide) (NGF), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011541518

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCCTCAT CTAATGGACA CTTTAATGAA GTTTTGGCCA GTGGTCGTGC AGTCCAAGGG 
GCTGGATGGC ATGCTGGACC CAAGCTCAGC TCAGCGTCCG GACCCAATAA CAGTTTTACC
AAGGGAGCAG CTTTCTATCC TGGCCACACT GAGGTGCATA GCGTAATGTC CATGTTGTTC
TACACTCTGA TCACAGCTTT TCTGATCGGC ATACAGGCGG AACCACACTC AGAGAGCAAT
GTCCCTGCAG GACACACCAT CCCCCAAGCC CACTGGACTA AACTTCAGCA TTCCCTTGAC
ACTGCCCTTC GCAGAGCCCG CAGCGCCCCG GCAGCGGCGA TAGCTGCACG CGTGGCGGGG
CAGACCCGCA ACATTACTGT GGACCCCAGG CTGTTTAAAA AGCGGCGACT CCGTTCACCC
CGTGTGCTGT TTAGCACCCA GCCTCCCCGT GAAGCTGCAG ACACTCAGGA TCTGGACTTC
GAGGTCGGTG GTGCTGCCCC CTTCAACAGG ACTCACAGGA GCAAGCGGTC ATCATCCCAT
CCCATCTTCC ACAGGGGCGA ATTCTCGGTG TGTGACAGTG TCAGCGTGTG GGTTGGGGAT
AAGACCACCG CCACAGACAT CAAGGGCAAG GAGGTGATGG TGTTGGGAGA GGTGAACATT
AACAACAGTG TATTCAAACA GTACTTTTTT GAGACCAAGT GCCGGGACCC AAATCCCGTT
GACAGCGGGT GCCGGGGCAT TGACTCAAAG CACTGGAACT CATATTGTAC CACGACTCAC
ACCTTTGTCA AGGCGCTGAC CATGGATGGC AAGCAGGCTG CCTGGCGGTT TATCCGGATA
GATACGGCCT GTGTGTGTGT GCTCAGCAGG AAGGCTGTGA GAAGAGCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25822
Accession Version XM_006710663.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 726bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product beta-nerve growth factor isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_032977.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578799178. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCCATGT TGTTCTACAC TCTGATCACA GCTTTTCTGA TCGGCATACA GGCGGAACCA 
CACTCAGAGA GCAATGTCCC TGCAGGACAC ACCATCCCCC AAGCCCACTG GACTAAACTT
CAGCATTCCC TTGACACTGC CCTTCGCAGA GCCCGCAGCG CCCCGGCAGC GGCGATAGCT
GCACGCGTGG CGGGGCAGAC CCGCAACATT ACTGTGGACC CCAGGCTGTT TAAAAAGCGG
CGACTCCGTT CACCCCGTGT GCTGTTTAGC ACCCAGCCTC CCCGTGAAGC TGCAGACACT
CAGGATCTGG ACTTCGAGGT CGGTGGTGCT GCCCCCTTCA ACAGGACTCA CAGGAGCAAG
CGGTCATCAT CCCATCCCAT CTTCCACAGG GGCGAATTCT CGGTGTGTGA CAGTGTCAGC
GTGTGGGTTG GGGATAAGAC CACCGCCACA GACATCAAGG GCAAGGAGGT GATGGTGTTG
GGAGAGGTGA ACATTAACAA CAGTGTATTC AAACAGTACT TTTTTGAGAC CAAGTGCCGG
GACCCAAATC CCGTTGACAG CGGGTGCCGG GGCATTGACT CAAAGCACTG GAACTCATAT
TGTACCACGA CTCACACCTT TGTCAAGGCG CTGACCATGG ATGGCAAGCA GGCTGCCTGG
CGGTTTATCC GGATAGATAC GGCCTGTGTG TGTGTGCTCA GCAGGAAGGC TGTGAGAAGA
GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_006710726.1
CDS33..758
Misc Feature(1)405..749(+)
Translation
Position Chain Variation Link
3 3 c, g dbSNP:532927301
17 17 a, c dbSNP:538280316
26 26 c, t dbSNP:376149287
28 28 a, g dbSNP:745683574
29 29 c, g dbSNP:781065610
30 30 a, g, t dbSNP:750055928
34 34 c, t dbSNP:751638085
43 43 c, t dbSNP:777773454
50 50 c, t dbSNP:370169329
74 74 c, t dbSNP:752800027
75 75 a, c, g dbSNP:181255687
85 85 c, t dbSNP:754287903
86 86 a, g dbSNP:6325
87 87 a, g dbSNP:761319558
91 91 c, t dbSNP:773903815
101 101 a, g, t dbSNP:762536454
110 110 c, t dbSNP:150188752
115 115 c, t dbSNP:769465872
122 122 c, t dbSNP:745630230
136 136 c, t dbSNP:6330
138 138 c, t dbSNP:267597943
141 141 c, t dbSNP:770843971
150 150 c, g dbSNP:748028952
157 157 a, c dbSNP:746985577
160 160 c, t dbSNP:566033399
171 171 g, t dbSNP:185385214
172 172 a, c, t dbSNP:181709715
174 174 c, t dbSNP:779240755
177 177 c, t dbSNP:146716262
178 178 a, g dbSNP:781072056
182 182 a, g dbSNP:754238855
186 186 c, t dbSNP:766912679
187 187 a, g, t dbSNP:149054069
188 188 a, c dbSNP:750836533
191 191 c, t dbSNP:376241395
192 192 -, gccccgg dbSNP:768548505
192 192 a, g dbSNP:200459956
196 196 c, t dbSNP:775019570
197 197 a, c, g dbSNP:149876217
200 200 a, t dbSNP:776406021
202 202 c, t dbSNP:770647680
203 203 a, g dbSNP:746837424
205 205 c, t dbSNP:201861727
206 206 a, g dbSNP:147326889
208 208 c, t dbSNP:748148537
212 212 c, t dbSNP:779189568
216 216 c, t dbSNP:755243469
217 217 a, g dbSNP:369266810
218 218 c, t dbSNP:780432648
219 219 a, c, g dbSNP:750829893
220 220 c, t dbSNP:767878560
221 221 a, g dbSNP:757830779
223 223 a, c, t dbSNP:201087374
224 224 a, g dbSNP:759051358
225 225 a, g dbSNP:776208737
228 228 a, c dbSNP:766082334
233 233 c, g dbSNP:760350868
234 234 c, g, t dbSNP:572066909
235 235 a, g dbSNP:377253859
239 239 c, t dbSNP:372994894
240 240 a, g dbSNP:768794852
246 246 a, g dbSNP:11466110
251 251 c, t dbSNP:780383333
254 254 c, t dbSNP:756396299
256 256 a, g dbSNP:141486298
257 257 c, g, t dbSNP:757701792
261 261 c, t dbSNP:751997242
270 270 c, t dbSNP:778266438
271 271 a, g dbSNP:11466111
273 273 a, c dbSNP:753382007
275 275 a, g dbSNP:766849758
278 278 c, t dbSNP:765918245
279 279 c, t dbSNP:138175552
280 280 a, c, g dbSNP:767272925
283 283 c, t dbSNP:267597942
284 284 a, t dbSNP:761709969
288 288 c, t dbSNP:774379026
289 289 a, g, t dbSNP:200629339
294 294 c, t dbSNP:775686730
296 296 a, g dbSNP:769893063
297 297 c, t dbSNP:746169118
308 308 a, g dbSNP:202060337
315 315 c, t dbSNP:771270154
316 316 a, g dbSNP:150136942
324 324 a, g dbSNP:537372354
330 330 a, c dbSNP:758948909
331 331 c, t dbSNP:142538674
341 341 g, t dbSNP:372694067
347 347 c, t dbSNP:779678303
348 348 a, g dbSNP:755580704
350 350 a, g dbSNP:750030065
353 353 c, t dbSNP:202005277
354 354 a, g dbSNP:145894043
364 364 c, t dbSNP:751440257
367 367 a, c, g dbSNP:147763877
368 368 c, t dbSNP:35941329
374 374 c, t dbSNP:775429651
375 375 a, g dbSNP:376618971
395 395 a, g dbSNP:759547617
402 402 a, t dbSNP:145691700
411 411 a, c dbSNP:28758211
413 413 c, t dbSNP:372985633
419 419 c, t dbSNP:747348126
425 425 c, t dbSNP:778162180
426 426 a, g dbSNP:772557857
431 431 a, c dbSNP:746897874
434 434 a, g dbSNP:565841831
436 436 c, t dbSNP:755599709
449 449 c, t dbSNP:369555032
451 451 a, g dbSNP:201867223
452 452 a, c, t dbSNP:751386981
453 453 a, c, g dbSNP:199511298
460 460 a, t dbSNP:758273427
469 469 a, g dbSNP:752622800
471 471 -, aacc dbSNP:370724679
473 473 c, t dbSNP:765111580
474 474 a, c dbSNP:202138909
476 476 c, t dbSNP:759622419
477 477 a, g dbSNP:149823633
480 480 a, g dbSNP:766568424
498 498 a, g dbSNP:369927492
501 501 a, g dbSNP:773565185
514 514 c, g dbSNP:139830389
515 515 a, g dbSNP:748481624
519 519 a, g dbSNP:774878867
530 530 a, c dbSNP:769082615
536 536 c, t dbSNP:745359909
540 540 c, t dbSNP:139541754
544 544 a, g dbSNP:375808064
546 546 c, g dbSNP:746593757
562 562 c, t dbSNP:777582167
571 571 a, g dbSNP:758166016
584 584 c, t dbSNP:532714783
585 585 a, g dbSNP:141889164
588 588 a, g dbSNP:201152024
593 593 c, t dbSNP:779744960
594 594 a, c, g dbSNP:760753923
596 596 c, g dbSNP:773369123
624 624 a, g dbSNP:767755905
629 629 a, t dbSNP:762203206
632 632 c, t dbSNP:774626005
640 640 c, t dbSNP:769190598
641 641 a, c, g dbSNP:143157422
649 649 c, t dbSNP:770508381
662 662 a, g dbSNP:112292538
667 667 c, g dbSNP:777529073
677 677 c, t dbSNP:371529651
688 688 c, g dbSNP:371216746
693 693 c, t dbSNP:11466112
699 699 a, t dbSNP:80277627
709 709 a, g dbSNP:34542615
712 712 -, tagatac dbSNP:746752363
713 713 a, c, g dbSNP:565497625
716 716 c, t dbSNP:549646930
719 719 c, t dbSNP:750539380
721 721 c, t dbSNP:767703003
727 727 -, tg dbSNP:142696332
728 728 a, g dbSNP:761997516
731 731 c, g dbSNP:531522066
738 738 a, c dbSNP:561107153
741 741 g, t dbSNP:763390069
746 746 a, g dbSNP:776092330
755 755 c, t dbSNP:34079702
758 758 a, c dbSNP:770457149
759 759 c, g dbSNP:760233445
760 760 c, t dbSNP:772900409
764 764 a, c, g, t dbSNP:542460046
765 765 a, g dbSNP:768283373
767 767 c, t dbSNP:749107814
769 769 c, g, t dbSNP:199812295
770 770 a, g dbSNP:560280132
771 771 c, t dbSNP:545171466
776 776 -, t dbSNP:758257834
780 780 c, g dbSNP:757362434
792 792 a, g dbSNP:751673509
799 799 a, c, t dbSNP:763370576
833 833 c, t dbSNP:757967985
888 888 c, t dbSNP:749879805

Target ORF information:

RefSeq Version XM_006710663
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens nerve growth factor (beta polypeptide) (NGF), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006710663

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCCATGT TGTTCTACAC TCTGATCACA GCTTTTCTGA TCGGCATACA GGCGGAACCA 
CACTCAGAGA GCAATGTCCC TGCAGGACAC ACCATCCCCC AAGCCCACTG GACTAAACTT
CAGCATTCCC TTGACACTGC CCTTCGCAGA GCCCGCAGCG CCCCGGCAGC GGCGATAGCT
GCACGCGTGG CGGGGCAGAC CCGCAACATT ACTGTGGACC CCAGGCTGTT TAAAAAGCGG
CGACTCCGTT CACCCCGTGT GCTGTTTAGC ACCCAGCCTC CCCGTGAAGC TGCAGACACT
CAGGATCTGG ACTTCGAGGT CGGTGGTGCT GCCCCCTTCA ACAGGACTCA CAGGAGCAAG
CGGTCATCAT CCCATCCCAT CTTCCACAGG GGCGAATTCT CGGTGTGTGA CAGTGTCAGC
GTGTGGGTTG GGGATAAGAC CACCGCCACA GACATCAAGG GCAAGGAGGT GATGGTGTTG
GGAGAGGTGA ACATTAACAA CAGTGTATTC AAACAGTACT TTTTTGAGAC CAAGTGCCGG
GACCCAAATC CCGTTGACAG CGGGTGCCGG GGCATTGACT CAAAGCACTG GAACTCATAT
TGTACCACGA CTCACACCTT TGTCAAGGCG CTGACCATGG ATGGCAAGCA GGCTGCCTGG
CGGTTTATCC GGATAGATAC GGCCTGTGTG TGTGTGCTCA GCAGGAAGGC TGTGAGAAGA
GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25822
Accession Version XM_006710665.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 726bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product beta-nerve growth factor isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_032977.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578799182. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCCATGT TGTTCTACAC TCTGATCACA GCTTTTCTGA TCGGCATACA GGCGGAACCA 
CACTCAGAGA GCAATGTCCC TGCAGGACAC ACCATCCCCC AAGCCCACTG GACTAAACTT
CAGCATTCCC TTGACACTGC CCTTCGCAGA GCCCGCAGCG CCCCGGCAGC GGCGATAGCT
GCACGCGTGG CGGGGCAGAC CCGCAACATT ACTGTGGACC CCAGGCTGTT TAAAAAGCGG
CGACTCCGTT CACCCCGTGT GCTGTTTAGC ACCCAGCCTC CCCGTGAAGC TGCAGACACT
CAGGATCTGG ACTTCGAGGT CGGTGGTGCT GCCCCCTTCA ACAGGACTCA CAGGAGCAAG
CGGTCATCAT CCCATCCCAT CTTCCACAGG GGCGAATTCT CGGTGTGTGA CAGTGTCAGC
GTGTGGGTTG GGGATAAGAC CACCGCCACA GACATCAAGG GCAAGGAGGT GATGGTGTTG
GGAGAGGTGA ACATTAACAA CAGTGTATTC AAACAGTACT TTTTTGAGAC CAAGTGCCGG
GACCCAAATC CCGTTGACAG CGGGTGCCGG GGCATTGACT CAAAGCACTG GAACTCATAT
TGTACCACGA CTCACACCTT TGTCAAGGCG CTGACCATGG ATGGCAAGCA GGCTGCCTGG
CGGTTTATCC GGATAGATAC GGCCTGTGTG TGTGTGCTCA GCAGGAAGGC TGTGAGAAGA
GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_006710728.1
CDS157..882
Misc Feature(1)529..873(+)
Translation
Position Chain Variation Link
28 28 a, g dbSNP:747462818
61 61 a, c dbSNP:61730971
86 86 c, t dbSNP:756375071
87 87 a, g dbSNP:767567207
90 90 c, t dbSNP:535930103
91 91 a, g dbSNP:780222620
135 135 a, c dbSNP:572125330
150 150 c, t dbSNP:376149287
152 152 a, g dbSNP:745683574
153 153 c, g dbSNP:781065610
154 154 a, g, t dbSNP:750055928
158 158 c, t dbSNP:751638085
167 167 c, t dbSNP:777773454
174 174 c, t dbSNP:370169329
198 198 c, t dbSNP:752800027
199 199 a, c, g dbSNP:181255687
209 209 c, t dbSNP:754287903
210 210 a, g dbSNP:6325
211 211 a, g dbSNP:761319558
215 215 c, t dbSNP:773903815
225 225 a, g, t dbSNP:762536454
234 234 c, t dbSNP:150188752
239 239 c, t dbSNP:769465872
246 246 c, t dbSNP:745630230
260 260 c, t dbSNP:6330
262 262 c, t dbSNP:267597943
265 265 c, t dbSNP:770843971
274 274 c, g dbSNP:748028952
281 281 a, c dbSNP:746985577
284 284 c, t dbSNP:566033399
295 295 g, t dbSNP:185385214
296 296 a, c, t dbSNP:181709715
298 298 c, t dbSNP:779240755
301 301 c, t dbSNP:146716262
302 302 a, g dbSNP:781072056
306 306 a, g dbSNP:754238855
310 310 c, t dbSNP:766912679
311 311 a, g, t dbSNP:149054069
312 312 a, c dbSNP:750836533
315 315 c, t dbSNP:376241395
316 316 -, gccccgg dbSNP:768548505
316 316 a, g dbSNP:200459956
320 320 c, t dbSNP:775019570
321 321 a, c, g dbSNP:149876217
324 324 a, t dbSNP:776406021
326 326 c, t dbSNP:770647680
327 327 a, g dbSNP:746837424
329 329 c, t dbSNP:201861727
330 330 a, g dbSNP:147326889
332 332 c, t dbSNP:748148537
336 336 c, t dbSNP:779189568
340 340 c, t dbSNP:755243469
341 341 a, g dbSNP:369266810
342 342 c, t dbSNP:780432648
343 343 a, c, g dbSNP:750829893
344 344 c, t dbSNP:767878560
345 345 a, g dbSNP:757830779
347 347 a, c, t dbSNP:201087374
348 348 a, g dbSNP:759051358
349 349 a, g dbSNP:776208737
352 352 a, c dbSNP:766082334
357 357 c, g dbSNP:760350868
358 358 c, g, t dbSNP:572066909
359 359 a, g dbSNP:377253859
363 363 c, t dbSNP:372994894
364 364 a, g dbSNP:768794852
370 370 a, g dbSNP:11466110
375 375 c, t dbSNP:780383333
378 378 c, t dbSNP:756396299
380 380 a, g dbSNP:141486298
381 381 c, g, t dbSNP:757701792
385 385 c, t dbSNP:751997242
394 394 c, t dbSNP:778266438
395 395 a, g dbSNP:11466111
397 397 a, c dbSNP:753382007
399 399 a, g dbSNP:766849758
402 402 c, t dbSNP:765918245
403 403 c, t dbSNP:138175552
404 404 a, c, g dbSNP:767272925
407 407 c, t dbSNP:267597942
408 408 a, t dbSNP:761709969
412 412 c, t dbSNP:774379026
413 413 a, g, t dbSNP:200629339
418 418 c, t dbSNP:775686730
420 420 a, g dbSNP:769893063
421 421 c, t dbSNP:746169118
432 432 a, g dbSNP:202060337
439 439 c, t dbSNP:771270154
440 440 a, g dbSNP:150136942
448 448 a, g dbSNP:537372354
454 454 a, c dbSNP:758948909
455 455 c, t dbSNP:142538674
465 465 g, t dbSNP:372694067
471 471 c, t dbSNP:779678303
472 472 a, g dbSNP:755580704
474 474 a, g dbSNP:750030065
477 477 c, t dbSNP:202005277
478 478 a, g dbSNP:145894043
488 488 c, t dbSNP:751440257
491 491 a, c, g dbSNP:147763877
492 492 c, t dbSNP:35941329
498 498 c, t dbSNP:775429651
499 499 a, g dbSNP:376618971
519 519 a, g dbSNP:759547617
526 526 a, t dbSNP:145691700
535 535 a, c dbSNP:28758211
537 537 c, t dbSNP:372985633
543 543 c, t dbSNP:747348126
549 549 c, t dbSNP:778162180
550 550 a, g dbSNP:772557857
555 555 a, c dbSNP:746897874
558 558 a, g dbSNP:565841831
560 560 c, t dbSNP:755599709
573 573 c, t dbSNP:369555032
575 575 a, g dbSNP:201867223
576 576 a, c, t dbSNP:751386981
577 577 a, c, g dbSNP:199511298
584 584 a, t dbSNP:758273427
593 593 a, g dbSNP:752622800
595 595 -, aacc dbSNP:370724679
597 597 c, t dbSNP:765111580
598 598 a, c dbSNP:202138909
600 600 c, t dbSNP:759622419
601 601 a, g dbSNP:149823633
604 604 a, g dbSNP:766568424
622 622 a, g dbSNP:369927492
625 625 a, g dbSNP:773565185
638 638 c, g dbSNP:139830389
639 639 a, g dbSNP:748481624
643 643 a, g dbSNP:774878867
654 654 a, c dbSNP:769082615
660 660 c, t dbSNP:745359909
664 664 c, t dbSNP:139541754
668 668 a, g dbSNP:375808064
670 670 c, g dbSNP:746593757
686 686 c, t dbSNP:777582167
695 695 a, g dbSNP:758166016
708 708 c, t dbSNP:532714783
709 709 a, g dbSNP:141889164
712 712 a, g dbSNP:201152024
717 717 c, t dbSNP:779744960
718 718 a, c, g dbSNP:760753923
720 720 c, g dbSNP:773369123
748 748 a, g dbSNP:767755905
753 753 a, t dbSNP:762203206
756 756 c, t dbSNP:774626005
764 764 c, t dbSNP:769190598
765 765 a, c, g dbSNP:143157422
773 773 c, t dbSNP:770508381
786 786 a, g dbSNP:112292538
791 791 c, g dbSNP:777529073
801 801 c, t dbSNP:371529651
812 812 c, g dbSNP:371216746
817 817 c, t dbSNP:11466112
823 823 a, t dbSNP:80277627
833 833 a, g dbSNP:34542615
836 836 -, tagatac dbSNP:746752363
837 837 a, c, g dbSNP:565497625
840 840 c, t dbSNP:549646930
843 843 c, t dbSNP:750539380
845 845 c, t dbSNP:767703003
851 851 -, tg dbSNP:142696332
852 852 a, g dbSNP:761997516
855 855 c, g dbSNP:531522066
862 862 a, c dbSNP:561107153
865 865 g, t dbSNP:763390069
870 870 a, g dbSNP:776092330
879 879 c, t dbSNP:34079702
882 882 a, c dbSNP:770457149
883 883 c, g dbSNP:760233445
884 884 c, t dbSNP:772900409
888 888 a, c, g, t dbSNP:542460046
889 889 a, g dbSNP:768283373
891 891 c, t dbSNP:749107814
893 893 c, g, t dbSNP:199812295
894 894 a, g dbSNP:560280132
895 895 c, t dbSNP:545171466
900 900 -, t dbSNP:758257834
904 904 c, g dbSNP:757362434
916 916 a, g dbSNP:751673509
923 923 a, c, t dbSNP:763370576
957 957 c, t dbSNP:757967985
1012 1012 c, t dbSNP:749879805

Target ORF information:

RefSeq Version XM_006710665
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens nerve growth factor (beta polypeptide) (NGF), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006710665

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCCATGT TGTTCTACAC TCTGATCACA GCTTTTCTGA TCGGCATACA GGCGGAACCA 
CACTCAGAGA GCAATGTCCC TGCAGGACAC ACCATCCCCC AAGCCCACTG GACTAAACTT
CAGCATTCCC TTGACACTGC CCTTCGCAGA GCCCGCAGCG CCCCGGCAGC GGCGATAGCT
GCACGCGTGG CGGGGCAGAC CCGCAACATT ACTGTGGACC CCAGGCTGTT TAAAAAGCGG
CGACTCCGTT CACCCCGTGT GCTGTTTAGC ACCCAGCCTC CCCGTGAAGC TGCAGACACT
CAGGATCTGG ACTTCGAGGT CGGTGGTGCT GCCCCCTTCA ACAGGACTCA CAGGAGCAAG
CGGTCATCAT CCCATCCCAT CTTCCACAGG GGCGAATTCT CGGTGTGTGA CAGTGTCAGC
GTGTGGGTTG GGGATAAGAC CACCGCCACA GACATCAAGG GCAAGGAGGT GATGGTGTTG
GGAGAGGTGA ACATTAACAA CAGTGTATTC AAACAGTACT TTTTTGAGAC CAAGTGCCGG
GACCCAAATC CCGTTGACAG CGGGTGCCGG GGCATTGACT CAAAGCACTG GAACTCATAT
TGTACCACGA CTCACACCTT TGTCAAGGCG CTGACCATGG ATGGCAAGCA GGCTGCCTGG
CGGTTTATCC GGATAGATAC GGCCTGTGTG TGTGTGCTCA GCAGGAAGGC TGTGAGAAGA
GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25822
Accession Version NM_002506.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 726bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product beta-nerve growth factor precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X52599.1, BC032517.2, CR541855.1 and BE045524.1. This sequence is a reference standard in the RefSeqGene project. On Jul 19, 2005 this sequence version replaced gi:4505390. Summary: This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X52599.1, BC032517.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCCATGT TGTTCTACAC TCTGATCACA GCTTTTCTGA TCGGCATACA GGCGGAACCA 
CACTCAGAGA GCAATGTCCC TGCAGGACAC ACCATCCCCC AAGCCCACTG GACTAAACTT
CAGCATTCCC TTGACACTGC CCTTCGCAGA GCCCGCAGCG CCCCGGCAGC GGCGATAGCT
GCACGCGTGG CGGGGCAGAC CCGCAACATT ACTGTGGACC CCAGGCTGTT TAAAAAGCGG
CGACTCCGTT CACCCCGTGT GCTGTTTAGC ACCCAGCCTC CCCGTGAAGC TGCAGACACT
CAGGATCTGG ACTTCGAGGT CGGTGGTGCT GCCCCCTTCA ACAGGACTCA CAGGAGCAAG
CGGTCATCAT CCCATCCCAT CTTCCACAGG GGCGAATTCT CGGTGTGTGA CAGTGTCAGC
GTGTGGGTTG GGGATAAGAC CACCGCCACA GACATCAAGG GCAAGGAGGT GATGGTGTTG
GGAGAGGTGA ACATTAACAA CAGTGTATTC AAACAGTACT TTTTTGAGAC CAAGTGCCGG
GACCCAAATC CCGTTGACAG CGGGTGCCGG GGCATTGACT CAAAGCACTG GAACTCATAT
TGTACCACGA CTCACACCTT TGTCAAGGCG CTGACCATGG ATGGCAAGCA GGCTGCCTGG
CGGTTTATCC GGATAGATAC GGCCTGTGTG TGTGTGCTCA GCAGGAAGGC TGTGAGAAGA
GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_002497.2
CDS170..895
Misc Feature(1)419..421(+)
Misc Feature(2)542..886(+)
Exon (1)1..33
Gene:NGF
Gene Synonym:
Exon (2)34..157
Gene:NGF
Gene Synonym:
Exon (3)158..1049
Gene:NGF
Gene Synonym:
Translation
Position Chain Variation Link
2 2 a, g dbSNP:551282986
16 16 c, g dbSNP:532927301
30 30 a, c dbSNP:538280316
41 41 a, g dbSNP:747462818
74 74 a, c dbSNP:61730971
99 99 c, t dbSNP:756375071
100 100 a, g dbSNP:767567207
103 103 c, t dbSNP:535930103
104 104 a, g dbSNP:780222620
148 148 a, c dbSNP:572125330
163 163 c, t dbSNP:376149287
165 165 a, g dbSNP:745683574
166 166 c, g dbSNP:781065610
167 167 a, g, t dbSNP:750055928
171 171 c, t dbSNP:751638085
180 180 c, t dbSNP:777773454
187 187 c, t dbSNP:370169329
211 211 c, t dbSNP:752800027
212 212 a, c, g dbSNP:181255687
222 222 c, t dbSNP:754287903
223 223 a, g dbSNP:6325
224 224 a, g dbSNP:761319558
228 228 c, t dbSNP:773903815
238 238 a, g, t dbSNP:762536454
247 247 c, t dbSNP:150188752
252 252 c, t dbSNP:769465872
259 259 c, t dbSNP:745630230
273 273 c, t dbSNP:6330
275 275 c, t dbSNP:267597943
278 278 c, t dbSNP:770843971
287 287 c, g dbSNP:748028952
294 294 a, c dbSNP:746985577
297 297 c, t dbSNP:566033399
308 308 g, t dbSNP:185385214
309 309 a, c, t dbSNP:181709715
311 311 c, t dbSNP:779240755
314 314 c, t dbSNP:146716262
315 315 a, g dbSNP:781072056
319 319 a, g dbSNP:754238855
323 323 c, t dbSNP:766912679
324 324 a, g, t dbSNP:149054069
325 325 a, c dbSNP:750836533
328 328 c, t dbSNP:376241395
329 329 -, gccccgg dbSNP:768548505
329 329 a, g dbSNP:200459956
333 333 c, t dbSNP:775019570
334 334 a, c, g dbSNP:149876217
337 337 a, t dbSNP:776406021
339 339 c, t dbSNP:770647680
340 340 a, g dbSNP:746837424
342 342 c, t dbSNP:201861727
343 343 a, g dbSNP:147326889
345 345 c, t dbSNP:748148537
349 349 c, t dbSNP:779189568
353 353 c, t dbSNP:755243469
354 354 a, g dbSNP:369266810
355 355 c, t dbSNP:780432648
356 356 a, c, g dbSNP:750829893
357 357 c, t dbSNP:767878560
358 358 a, g dbSNP:757830779
360 360 a, c, t dbSNP:201087374
361 361 a, g dbSNP:759051358
362 362 a, g dbSNP:776208737
365 365 a, c dbSNP:766082334
370 370 c, g dbSNP:760350868
371 371 c, g, t dbSNP:572066909
372 372 a, g dbSNP:377253859
376 376 c, t dbSNP:372994894
377 377 a, g dbSNP:768794852
383 383 a, g dbSNP:11466110
388 388 c, t dbSNP:780383333
391 391 c, t dbSNP:756396299
393 393 a, g dbSNP:141486298
394 394 c, g, t dbSNP:757701792
398 398 c, t dbSNP:751997242
407 407 c, t dbSNP:778266438
408 408 a, g dbSNP:11466111
410 410 a, c dbSNP:753382007
412 412 a, g dbSNP:766849758
415 415 c, t dbSNP:765918245
416 416 c, t dbSNP:138175552
417 417 a, c, g dbSNP:767272925
420 420 c, t dbSNP:267597942
421 421 a, t dbSNP:761709969
425 425 c, t dbSNP:774379026
426 426 a, g, t dbSNP:200629339
431 431 c, t dbSNP:775686730
433 433 a, g dbSNP:769893063
434 434 c, t dbSNP:746169118
445 445 a, g dbSNP:202060337
452 452 c, t dbSNP:771270154
453 453 a, g dbSNP:150136942
461 461 a, g dbSNP:537372354
467 467 a, c dbSNP:758948909
468 468 c, t dbSNP:142538674
478 478 g, t dbSNP:372694067
484 484 c, t dbSNP:779678303
485 485 a, g dbSNP:755580704
487 487 a, g dbSNP:750030065
490 490 c, t dbSNP:202005277
491 491 a, g dbSNP:145894043
501 501 c, t dbSNP:751440257
504 504 a, c, g dbSNP:147763877
505 505 c, t dbSNP:35941329
511 511 c, t dbSNP:775429651
512 512 a, g dbSNP:376618971
532 532 a, g dbSNP:759547617
539 539 a, t dbSNP:145691700
548 548 a, c dbSNP:28758211
550 550 c, t dbSNP:372985633
556 556 c, t dbSNP:747348126
562 562 c, t dbSNP:778162180
563 563 a, g dbSNP:772557857
568 568 a, c dbSNP:746897874
571 571 a, g dbSNP:565841831
573 573 c, t dbSNP:755599709
586 586 c, t dbSNP:369555032
588 588 a, g dbSNP:201867223
589 589 a, c, t dbSNP:751386981
590 590 a, c, g dbSNP:199511298
597 597 a, t dbSNP:758273427
606 606 a, g dbSNP:752622800
608 608 -, aacc dbSNP:370724679
610 610 c, t dbSNP:765111580
611 611 a, c dbSNP:202138909
613 613 c, t dbSNP:759622419
614 614 a, g dbSNP:149823633
617 617 a, g dbSNP:766568424
635 635 a, g dbSNP:369927492
638 638 a, g dbSNP:773565185
651 651 c, g dbSNP:139830389
652 652 a, g dbSNP:748481624
656 656 a, g dbSNP:774878867
667 667 a, c dbSNP:769082615
673 673 c, t dbSNP:745359909
677 677 c, t dbSNP:139541754
681 681 a, g dbSNP:375808064
683 683 c, g dbSNP:746593757
699 699 c, t dbSNP:777582167
708 708 a, g dbSNP:758166016
721 721 c, t dbSNP:532714783
722 722 a, g dbSNP:141889164
725 725 a, g dbSNP:201152024
730 730 c, t dbSNP:779744960
731 731 a, c, g dbSNP:760753923
733 733 c, g dbSNP:773369123
761 761 a, g dbSNP:767755905
766 766 a, t dbSNP:762203206
769 769 c, t dbSNP:774626005
777 777 c, t dbSNP:769190598
778 778 a, c, g dbSNP:143157422
786 786 c, t dbSNP:770508381
799 799 a, g dbSNP:112292538
804 804 c, g dbSNP:777529073
814 814 c, t dbSNP:371529651
825 825 c, g dbSNP:371216746
830 830 c, t dbSNP:11466112
836 836 a, t dbSNP:80277627
846 846 a, g dbSNP:34542615
849 849 -, tagatac dbSNP:746752363
850 850 a, c, g dbSNP:565497625
853 853 c, t dbSNP:549646930
856 856 c, t dbSNP:750539380
858 858 c, t dbSNP:767703003
864 864 -, tg dbSNP:142696332
865 865 a, g dbSNP:761997516
868 868 c, g dbSNP:531522066
875 875 a, c dbSNP:561107153
878 878 g, t dbSNP:763390069
883 883 a, g dbSNP:776092330
892 892 c, t dbSNP:34079702
895 895 a, c dbSNP:770457149
896 896 c, g dbSNP:760233445
897 897 c, t dbSNP:772900409
901 901 a, c, g, t dbSNP:542460046
902 902 a, g dbSNP:768283373
904 904 c, t dbSNP:749107814
906 906 c, g, t dbSNP:199812295
907 907 a, g dbSNP:560280132
908 908 c, t dbSNP:545171466
913 913 -, t dbSNP:758257834
917 917 c, g dbSNP:757362434
929 929 a, g dbSNP:751673509
936 936 a, c, t dbSNP:763370576
970 970 c, t dbSNP:757967985
1025 1025 c, t dbSNP:749879805

Target ORF information:

RefSeq Version NM_002506
Organism Homo sapiens (human)
Definition Homo sapiens nerve growth factor (beta polypeptide) (NGF), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002506

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCCATGT TGTTCTACAC TCTGATCACA GCTTTTCTGA TCGGCATACA GGCGGAACCA 
CACTCAGAGA GCAATGTCCC TGCAGGACAC ACCATCCCCC AAGCCCACTG GACTAAACTT
CAGCATTCCC TTGACACTGC CCTTCGCAGA GCCCGCAGCG CCCCGGCAGC GGCGATAGCT
GCACGCGTGG CGGGGCAGAC CCGCAACATT ACTGTGGACC CCAGGCTGTT TAAAAAGCGG
CGACTCCGTT CACCCCGTGT GCTGTTTAGC ACCCAGCCTC CCCGTGAAGC TGCAGACACT
CAGGATCTGG ACTTCGAGGT CGGTGGTGCT GCCCCCTTCA ACAGGACTCA CAGGAGCAAG
CGGTCATCAT CCCATCCCAT CTTCCACAGG GGCGAATTCT CGGTGTGTGA CAGTGTCAGC
GTGTGGGTTG GGGATAAGAC CACCGCCACA GACATCAAGG GCAAGGAGGT GATGGTGTTG
GGAGAGGTGA ACATTAACAA CAGTGTATTC AAACAGTACT TTTTTGAGAC CAAGTGCCGG
GACCCAAATC CCGTTGACAG CGGGTGCCGG GGCATTGACT CAAAGCACTG GAACTCATAT
TGTACCACGA CTCACACCTT TGTCAAGGCG CTGACCATGG ATGGCAAGCA GGCTGCCTGG
CGGTTTATCC GGATAGATAC GGCCTGTGTG TGTGTGCTCA GCAGGAAGGC TGTGAGAAGA
GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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