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CISD2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CISD2
Entrez Gene ID 493856
Full Name CDGSH iron sulfur domain 2
Synonyms ERIS, Miner1, NAF-1, WFS2, ZCD2
General protein information
Preferred Names
CDGSH iron-sulfur domain-containing protein 2
Names
CDGSH iron-sulfur domain-containing protein 2
mitoNEET related 1
mitoNEET-related 1 protein
zinc finger, CDGSH-type domain 2
nutrient-deprivation autophagy factor-1
endoplasmic reticulum intermembrane small protein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

4

4q24

Summary The protein encoded by this gene is a zinc finger protein that localizes to the endoplasmic reticulum. The encoded protein binds an iron/sulfur cluster and may be involved in calcium homeostasis. Defects in this gene are a cause of Wolfram syndrome 2. [provided by RefSeq, Mar 2011]. lac of sum
Disorder MIM:

611507

Disorder Html: Wolfram syndrome 2, 604928 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001008388 NP_001008389 CDGSH iron-sulfur domain-containing protein 2


Homo sapiens (human) CISD2 NP_001008389.1
Pan troglodytes (chimpanzee) CISD2 XP_003310469.1
Macaca mulatta (Rhesus monkey) LOC100429989 XP_002804204.1
Bos taurus (cattle) CISD2 NP_001073807.1
Mus musculus (house mouse) Cisd2 NP_080178.1
Rattus norvegicus (Norway rat) Cisd2 NP_001178537.1
Gallus gallus (chicken) CISD2 XP_420668.1
Danio rerio (zebrafish) cisd2 NP_956677.1
Drosophila melanogaster (fruit fly) CG1458 NP_651684.1
Xenopus (Silurana) tropicalis (western clawed frog) cisd2 NP_001011479.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CISD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CISD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu23185
NM_001008388 Homo sapiens CDGSH iron sulfur domain 2 (CISD2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu23185
Clone ID Related Accession (Same CDS sequence) NM_001008388
Accession Version NM_001008388.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 408bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product CDGSH iron-sulfur domain-containing protein 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018797.4, AK292134.1, BX537971.1 and AC083826.7. This sequence is a reference standard in the RefSeqGene project. On Jul 3, 2010 this sequence version replaced gi:197313633. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DN993861.1, BX537971.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
ATGGTGCTGG AGAGCGTGGC CCGTATCGTG AAGGTGCAGC TCCCTGCATA TCTGAAGCGG 
CTCCCAGTCC CTGAAAGCAT TACCGGGTTC GCTAGGCTCA CAGTTTCAGA ATGGCTTCGG
TTATTGCCTT TCCTTGGTGT ACTCGCACTT CTTGGCTACC TTGCAGTTCG TCCATTCCTC
CCGAAGAAGA AACAACAGAA GGATAGCTTG ATTAATCTTA AAATACAAAA GGAAAATCCG
AAAGTAGTGA ATGAAATAAA CATTGAAGAT TTGTGTCTTA CTAAAGCAGC TTATTGTAGG
TGTTGGCGTT CTAAAACGTT TCCTGCCTGC GATGGTTCAC ATAATAAACA CAATGAATTG
ACAGGAGATA ATGTGGGTCC ACTAATACTG AAGAAGAAAG AAGTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001008389.1
CDS108..515
Misc Feature(1)114..305(+)
Misc Feature(2)219..287(+)
Misc Feature(3)348..464(+)
Exon (1)1..210
Gene:CISD2
Gene Synonym:
Exon (2)211..425
Gene:CISD2
Gene Synonym:
Exon (3)426..5892
Gene:CISD2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001008388
Organism Homo sapiens (human)
Definition Homo sapiens CDGSH iron sulfur domain 2 (CISD2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001008388

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGGTGCTGG AGAGCGTGGC CCGTATCGTG AAGGTGCAGC TCCCTGCATA TCTGAAGCGG 
CTCCCAGTCC CTGAAAGCAT TACCGGGTTC GCTAGGCTCA CAGTTTCAGA ATGGCTTCGG
TTATTGCCTT TCCTTGGTGT ACTCGCACTT CTTGGCTACC TTGCAGTTCG TCCATTCCTC
CCGAAGAAGA AACAACAGAA GGATAGCTTG ATTAATCTTA AAATACAAAA GGAAAATCCG
AAAGTAGTGA ATGAAATAAA CATTGAAGAT TTGTGTCTTA CTAAAGCAGC TTATTGTAGG
TGTTGGCGTT CTAAAACGTT TCCTGCCTGC GATGGTTCAC ATAATAAACA CAATGAATTG
ACAGGAGATA ATGTGGGTCC ACTAATACTG AAGAAGAAAG AAGTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

NAF-1 and mitoNEET are central to human breast cancer proliferation by maintaining mitochondrial homeostasis and promoting tumor growth
Proc. Natl. Acad. Sci. U.S.A. 110 (36), 14676-14681 (2013)
Sohn YS, Tamir S, Song L, Michaeli D, Matouk I, Conlan AR, Harir Y, Holt SH, Shulaev V, Paddock ML, Hochberg A, Cabanchick IZ, Onuchic JN, Jennings PA, Nechushtai R and Mittler R.


book

A role for the CISD2 gene in lifespan control and human disease
Ann. N. Y. Acad. Sci. 1201, 58-64 (2010)
Chen YF, Wu CY, Kirby R, Kao CH and Tsai TF.


book

Antagonism of Beclin 1-dependent autophagy by BCL-2 at the endoplasmic reticulum requires NAF-1
EMBO J. 29 (3), 606-618 (2010)
Chang NC, Nguyen M, Germain M and Shore GC.


book

Crystal structure of Miner1: The redox-active 2Fe-2S protein causative in Wolfram Syndrome 2
J. Mol. Biol. 392 (1), 143-153 (2009)
Conlan AR, Axelrod HL, Cohen AE, Abresch EC, Zuris J, Yee D, Nechushtai R, Jennings PA and Paddock ML.


book

A homozygous mutation in a novel zinc-finger protein, ERIS, is responsible for Wolfram syndrome 2
Am. J. Hum. Genet. 81 (4), 673-683 (2007)
Amr S, Heisey C, Zhang M, Xia XJ, Shows KH, Ajlouni K, Pandya A, Satin LS, El-Shanti H and Shiang R.


book

MitoNEET is an iron-containing outer mitochondrial membrane protein that regulates oxidative capacity
Proc. Natl. Acad. Sci. U.S.A. 104 (13), 5318-5323 (2007)
Wiley SE, Murphy AN, Ross SA, van der Geer P and Dixon JE.


book

Complete sequencing and characterization of 21,243 full-length human cDNAs
Nat. Genet. 36 (1), 40-45 (2004)
Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T and Sugano S.


book

Wolfram syndrome: phenotype and novel mutation in two Taiwanese siblings
J. Formos. Med. Assoc. 102 (11), 808-811 (2003)
Shu SG, Tsai CR and Chi CS.


book

Bleeding tendency in Wolfram syndrome: a newly identified feature with phenotype genotype correlation
Eur. J. Pediatr. 160 (4), 243-246 (2001)
al-Sheyyab M, Jarrah N, Younis E, Shennak MM, Hadidi A, Awidi A, el-Shanti H and Ajlouni K.


book

Homozygosity mapping identifies an additional locus for Wolfram syndrome on chromosome 4q
Am. J. Hum. Genet. 66 (4), 1229-1236 (2000)
El-Shanti H, Lidral AC, Jarrah N, Druhan L and Ajlouni K.


 
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