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OTX2 orthodenticle homeobox 2 [Homo sapiens (human)]

Gene Symbol OTX2
Entrez Gene ID 5015
Full Name orthodenticle homeobox 2
Synonyms CPHD6, MCOPS5
General protein information
Preferred Names
homeobox protein OTX2
Names
homeobox protein OTX2
orthodenticle homolog 2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

14

14q22.3

Summary This gene encodes a member of the bicoid subfamily of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and plays a role in brain, craniofacial, and sensory organ development. The encoded protein also influences the proliferation and differentiation of dopaminergic neuronal progenitor cells during mitosis. Mutations in this gene cause syndromic microphthalmia 5 (MCOPS5) and combined pituitary hormone deficiency 6 (CPHD6). This gene is also suspected of having an oncogenic role in medulloblastoma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Pseudogenes of this gene are known to exist on chromosomes two and nine. [provided by RefSeq, Jul 2012]. lac of sum
Disorder MIM:

600037

Disorder Html: Microphthalmia, syndromic 5, 610125 (3)
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu16734 NM_021728 Homo sapiens orthodenticle homeobox 2 (OTX2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu16931 NM_172337 Homo sapiens orthodenticle homeobox 2 (OTX2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu16931 NM_001270523 Homo sapiens orthodenticle homeobox 2 (OTX2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu16931 NM_001270524 Homo sapiens orthodenticle homeobox 2 (OTX2), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu16734 NM_001270525 Homo sapiens orthodenticle homeobox 2 (OTX2), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu16734D
Sequence Information ORF Nucleotide Sequence (Length: 894bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product homeobox protein OTX2 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB593058.1 and BC032579.1. On Jul 14, 2012 this sequence version replaced gi:27436931. Summary: This gene encodes a member of the bicoid subfamily of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and plays a role in brain, craniofacial, and sensory organ development. The encoded protein also influences the proliferation and differentiation of dopaminergic neuronal progenitor cells during mitosis. Mutations in this gene cause syndromic microphthalmia 5 (MCOPS5) and combined pituitary hormone deficiency 6 (CPHD6). This gene is also suspected of having an oncogenic role in medulloblastoma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Pseudogenes of this gene are known to exist on chromosomes two and nine. [provided by RefSeq, Jul 2012]. Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1 and 5 encode the same protein (isoform a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032579.1, BE779002.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2148093, SAMEA2151119 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGATGTCTT ATCTTAAGCA ACCGCCTTAC GCAGTCAATG GGCTGAGTCT GACCACTTCG 
GGTATGGACT TGCTGCACCC CTCCGTGGGC TACCCGGGGC CCTGGGCTTC TTGTCCCGCA
GCCACCCCCC GGAAACAGCG CCGGGAGAGG ACGACGTTCA CTCGGGCGCA GCTAGATGTG
CTGGAAGCAC TGTTTGCCAA GACCCGGTAC CCAGACATCT TCATGCGAGA GGAGGTGGCA
CTGAAAATCA ACTTGCCCGA GTCGAGGGTG CAGGTATGGT TTAAGAATCG AAGAGCTAAG
TGCCGCCAAC AACAGCAACA ACAGCAGAAT GGAGGTCAAA ACAAAGTGAG ACCTGCCAAA
AAGAAGACAT CTCCAGCTCG GGAAGTGAGT TCAGAGAGTG GAACAAGTGG CCAATTCACT
CCCCCCTCTA GCACCTCAGT CCCGACCATT GCCAGCAGCA GTGCTCCTGT GTCTATCTGG
AGCCCAGCTT CCATCTCCCC ACTGTCAGAT CCCTTGTCCA CCTCCTCTTC CTGCATGCAG
AGGTCCTATC CCATGACCTA TACTCAGGCT TCAGGTTATA GTCAAGGATA TGCTGGCTCA
ACTTCCTACT TTGGGGGCAT GGACTGTGGA TCATATTTGA CCCCTATGCA TCACCAGCTT
CCCGGACCAG GGGCCACACT CAGTCCCATG GGTACCAATG CAGTCACCAG CCATCTCAAT
CAGTCCCCAG CTTCTCTTTC CACCCAGGGA TATGGAGCTT CAAGCTTGGG TTTTAACTCA
ACCACTGATT GCTTGGATTA TAAGGACCAA ACTGCCTCCT GGAAGCTTAA CTTCAATGCT
GACTGCTTGG ATTATAAAGA TCAGACATCC TCGTGGAAAT TCCAGGTTTT GTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_068374.1
CDS285..1178
Misc Feature(1)162..164(+)
Misc Feature(2)423..590(+)
Misc Feature(3)423..590(+)
Misc Feature(4)429..581(+)
Misc Feature(5)765..1013(+)
Exon (1)1..101
Gene:OTX2
Gene Synonym:
Exon (2)102..165
Gene:OTX2
Gene Synonym:
Exon (3)166..381
Gene:OTX2
Gene Synonym:
Exon (4)382..557
Gene:OTX2
Gene Synonym:
Exon (5)558..2206
Gene:OTX2
Gene Synonym:
Translation
Position Chain Variation Link
22 22 c, g dbSNP:563987229
23 23 a, g dbSNP:746287642
80 80 c, t dbSNP:535146235
81 81 c, t dbSNP:566447220
88 88 g, t dbSNP:200705423
92 92 c, t dbSNP:773133418
97 97 a, g dbSNP:138281551
98 98 c, t dbSNP:185374570
111 111 c, t dbSNP:150887670
158 158 g, t dbSNP:780689107
193 193 c, g dbSNP:566671671
203 203 c, g dbSNP:540831263
234 234 a, t dbSNP:546858272
235 235 a, g dbSNP:747816534
238 238 c, g dbSNP:776104541
239 239 a, g dbSNP:200002729
241 241 c, t dbSNP:373972732
242 242 a, g dbSNP:564618315
244 244 c, t dbSNP:779606022
247 247 c, t dbSNP:758339288
252 252 c, t dbSNP:546311189
256 256 a, c dbSNP:370535121
258 258 c, g dbSNP:757069643
264 264 c, g dbSNP:754146678
268 268 c, t dbSNP:764157150
276 276 a, g dbSNP:756345000
278 278 a, c dbSNP:752785578
288 288 a, g dbSNP:767603643
293 293 c, t dbSNP:575736431
299 299 g, t dbSNP:752010967
309 309 c, t dbSNP:766756721
315 315 g, t dbSNP:763234678
322 322 a, g dbSNP:776356711
323 323 c, t dbSNP:768254721
326 326 g, t dbSNP:760374061
341 341 c, t dbSNP:774907916
342 342 c, t dbSNP:771687556
346 346 a, g dbSNP:745822138
347 347 -, t dbSNP:779418668
348 348 a, g dbSNP:200680654
350 350 c, g dbSNP:778956556
353 353 a, c dbSNP:770855748
360 360 c, g dbSNP:749151724
365 365 -, c dbSNP:786205874
369 369 a, g dbSNP:777618282
373 373 a, g dbSNP:756331034
379 379 c, t dbSNP:752941534
384 384 c, t dbSNP:371205339
390 390 c, g dbSNP:761773984
399 399 a, c dbSNP:751692939
402 402 a, g dbSNP:780106127
409 409 a, c dbSNP:758946986
410 410 c, t dbSNP:750923926
411 411 c, g dbSNP:374042850
414 414 -, c dbSNP:758119168
446 446 c, t dbSNP:757602259
460 460 a, g dbSNP:567955379
471 471 g, t dbSNP:368759331
476 476 a, g dbSNP:141664597
487 487 c, t dbSNP:759256101
488 488 c, t dbSNP:774043425
491 491 a, g dbSNP:765964221
497 497 a, g dbSNP:762821214
510 510 a, c dbSNP:773157352
542 542 c, t dbSNP:769477143
543 543 a, g dbSNP:786205224
548 548 a, g dbSNP:376651816
554 554 g, t dbSNP:747916036
573 573 c, g dbSNP:104894464
574 574 a, g dbSNP:200990681
591 591 a, c dbSNP:78953717
593 593 a, g dbSNP:749788509
600 600 -, c dbSNP:786205884
614 614 c, t dbSNP:778304481
620 620 c, t dbSNP:536495343
625 625 a, g dbSNP:751258203
634 634 c, g dbSNP:779696471
635 635 a, c dbSNP:757971368
636 636 a, c dbSNP:750013761
646 646 a, g dbSNP:764625033
650 650 a, g dbSNP:761862286
655 655 c, t dbSNP:753729345
656 656 c, t dbSNP:763911822
657 657 c, t dbSNP:760396081
663 663 c, t dbSNP:775702810
664 664 a, c, g dbSNP:199799627
670 670 g, t dbSNP:774537529
672 672 a, g dbSNP:748205899
680 680 a, g dbSNP:749843466
685 685 c, g dbSNP:778357805
690 690 a, g dbSNP:770291855
695 695 c, t dbSNP:772021946
696 696 c, t dbSNP:74658806
702 702 a, c, g dbSNP:758106958
705 705 a, c dbSNP:376333965
707 707 a, c, t dbSNP:372008404
708 708 c, g dbSNP:753783256
709 709 a, c, g dbSNP:199761861
710 710 c, t dbSNP:553884027
721 721 c, g dbSNP:786205879
727 727 c, t dbSNP:201721391
728 728 a, c, g dbSNP:147896150
730 730 c, t dbSNP:771047348
731 731 c, t dbSNP:763064579
735 735 a, g, t dbSNP:770486100
736 736 c, t dbSNP:748631596
737 737 c, t dbSNP:777104497
739 739 a, g dbSNP:768857621
743 743 c, t dbSNP:34537598
748 748 c, g, t dbSNP:200171280
761 761 a, c dbSNP:373798705
767 767 c, t dbSNP:538192166
772 772 -, gc dbSNP:786205873
778 778 a, t dbSNP:756087368
788 788 g, t dbSNP:752526255
798 798 a, t dbSNP:370354056
809 809 c, t dbSNP:767374937
824 824 a, g dbSNP:368033398
834 834 c, t dbSNP:751820068
836 836 a, c dbSNP:766762080
845 845 a, c, t dbSNP:104894465
858 858 g, t dbSNP:773463323
864 864 a, g dbSNP:765382721
865 865 a, g dbSNP:762435469
868 868 a, g dbSNP:776964451
870 870 g, t dbSNP:397514463
871 871 g, t dbSNP:142205965
875 875 c, t dbSNP:747279627
881 881 c, g dbSNP:775913832
885 885 a, g, t dbSNP:748910557
886 886 a, c, t dbSNP:755578825
925 925 a, c dbSNP:150982073
927 927 a, c dbSNP:780929725
939 939 c, g dbSNP:754929157
940 940 a, c dbSNP:751376563
945 945 c, t dbSNP:548455339
947 947 c, t dbSNP:142743327
948 948 a, g dbSNP:374968145
957 957 a, g dbSNP:750626422
961 961 c, t dbSNP:765441049
964 964 g, t dbSNP:761733794
966 966 a, g dbSNP:529946021
969 969 -, cccatgggt dbSNP:745480462
977 977 c, t dbSNP:764571884
982 982 a, g dbSNP:370761964
989 989 c, t dbSNP:775876318
997 997 a, g, t dbSNP:144449264
999 999 c, t dbSNP:772782540
1008 1008 c, t dbSNP:769304167
1015 1015 c, t dbSNP:747665958
1023 1023 c, t dbSNP:143131956
1027 1027 c, t dbSNP:199650198
1032 1032 c, g dbSNP:768514532
1033 1033 c, g dbSNP:746941491
1041 1041 a, g dbSNP:139800030
1042 1042 c, t dbSNP:758228717
1048 1048 a, g dbSNP:781096873
1066 1066 c, t dbSNP:750679604
1081 1081 a, c dbSNP:779220317
1115 1115 c, t dbSNP:78559885
1124 1124 c, t dbSNP:201396096
1128 1128 a, t dbSNP:764205900
1150 1150 c, t dbSNP:761138937
1156 1156 c, t dbSNP:753156713
1157 1157 a, c, g dbSNP:759833842
1165 1165 c, t dbSNP:774680300
1180 1180 a, g dbSNP:370275924
1183 1183 a, c, t dbSNP:776053080
1188 1188 a, g dbSNP:171978
1204 1204 a, c, g dbSNP:780072062
1208 1208 c, t dbSNP:772006682
1212 1212 a, t dbSNP:182392988
1219 1219 a, g dbSNP:3742576
1221 1221 g, t dbSNP:558072316
1224 1224 a, g dbSNP:757464106
1299 1299 a, g dbSNP:575988911
1302 1302 c, t dbSNP:555242082
1303 1303 c, t dbSNP:561466216
1304 1304 a, c, g dbSNP:750146313
1324 1324 c, t dbSNP:543291377
1338 1338 a, g dbSNP:75947588
1341 1341 a, g dbSNP:764880954
1439 1439 c, g dbSNP:553969803
1451 1451 c, t dbSNP:538805998
1494 1494 a, g dbSNP:142727455
1517 1517 c, t dbSNP:556614831
1518 1518 -, c dbSNP:3215889
1523 1523 a, c dbSNP:537889967
1537 1537 g, t dbSNP:567634820
1565 1565 g, t dbSNP:372333803
1602 1602 a, c dbSNP:778705827
1605 1605 a, g dbSNP:761387465
1614 1614 c, g dbSNP:190556214
1669 1669 a, g dbSNP:369779296
1670 1670 -, attg dbSNP:149455658
1687 1687 a, g dbSNP:754230866
1773 1773 a, g dbSNP:764392879
1811 1811 c, t dbSNP:536571793
1845 1845 a, c dbSNP:184421963
1855 1855 c, t dbSNP:56245644
1876 1876 a, t dbSNP:58246365
1900 1900 a, g dbSNP:192422162
1942 1942 a, g dbSNP:775857186
1957 1957 a, g dbSNP:138197536
2000 2000 a, t dbSNP:187055500
2006 2006 a, g dbSNP:772253161
2036 2036 a, g dbSNP:549838567
2097 2097 c, g dbSNP:74517617
2098 2098 a, g dbSNP:80140046
2099 2099 a, t dbSNP:78935901
2100 2100 c, t dbSNP:759551235
2103 2103 c, t dbSNP:781146554
2136 2136 a, g dbSNP:767628237
2144 2144 a, g dbSNP:57390094
2186 2186 a, g dbSNP:184224534

Target ORF information:

RefSeq Version NM_021728
Organism Homo sapiens (human)
Definition Homo sapiens orthodenticle homeobox 2 (OTX2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_021728

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGATGTCTT ATCTTAAGCA ACCGCCTTAC GCAGTCAATG GGCTGAGTCT GACCACTTCG 
GGTATGGACT TGCTGCACCC CTCCGTGGGC TACCCGGGGC CCTGGGCTTC TTGTCCCGCA
GCCACCCCCC GGAAACAGCG CCGGGAGAGG ACGACGTTCA CTCGGGCGCA GCTAGATGTG
CTGGAAGCAC TGTTTGCCAA GACCCGGTAC CCAGACATCT TCATGCGAGA GGAGGTGGCA
CTGAAAATCA ACTTGCCCGA GTCGAGGGTG CAGGTATGGT TTAAGAATCG AAGAGCTAAG
TGCCGCCAAC AACAGCAACA ACAGCAGAAT GGAGGTCAAA ACAAAGTGAG ACCTGCCAAA
AAGAAGACAT CTCCAGCTCG GGAAGTGAGT TCAGAGAGTG GAACAAGTGG CCAATTCACT
CCCCCCTCTA GCACCTCAGT CCCGACCATT GCCAGCAGCA GTGCTCCTGT GTCTATCTGG
AGCCCAGCTT CCATCTCCCC ACTGTCAGAT CCCTTGTCCA CCTCCTCTTC CTGCATGCAG
AGGTCCTATC CCATGACCTA TACTCAGGCT TCAGGTTATA GTCAAGGATA TGCTGGCTCA
ACTTCCTACT TTGGGGGCAT GGACTGTGGA TCATATTTGA CCCCTATGCA TCACCAGCTT
CCCGGACCAG GGGCCACACT CAGTCCCATG GGTACCAATG CAGTCACCAG CCATCTCAAT
CAGTCCCCAG CTTCTCTTTC CACCCAGGGA TATGGAGCTT CAAGCTTGGG TTTTAACTCA
ACCACTGATT GCTTGGATTA TAAGGACCAA ACTGCCTCCT GGAAGCTTAA CTTCAATGCT
GACTGCTTGG ATTATAAAGA TCAGACATCC TCGTGGAAAT TCCAGGTTTT GTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu16931D
Sequence Information ORF Nucleotide Sequence (Length: 870bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product homeobox protein OTX2 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB593056.1, AB593057.1 and BC032579.1. On Jul 14, 2012 this sequence version replaced gi:27436932. Summary: This gene encodes a member of the bicoid subfamily of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and plays a role in brain, craniofacial, and sensory organ development. The encoded protein also influences the proliferation and differentiation of dopaminergic neuronal progenitor cells during mitosis. Mutations in this gene cause syndromic microphthalmia 5 (MCOPS5) and combined pituitary hormone deficiency 6 (CPHD6). This gene is also suspected of having an oncogenic role in medulloblastoma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Pseudogenes of this gene are known to exist on chromosomes two and nine. [provided by RefSeq, Jul 2012]. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an in-frame segment of the coding region, compared to variant 1, and, compared to isoform a, encodes a shorter isoform (b). Variants 2-4 encode the same protein (isoform b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CX753473.1, AB593057.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA962344 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGATGTCTT ATCTTAAGCA ACCGCCTTAC GCAGTCAATG GGCTGAGTCT GACCACTTCG 
GGTATGGACT TGCTGCACCC CTCCGTGGGC TACCCGGCCA CCCCCCGGAA ACAGCGCCGG
GAGAGGACGA CGTTCACTCG GGCGCAGCTA GATGTGCTGG AAGCACTGTT TGCCAAGACC
CGGTACCCAG ACATCTTCAT GCGAGAGGAG GTGGCACTGA AAATCAACTT GCCCGAGTCG
AGGGTGCAGG TATGGTTTAA GAATCGAAGA GCTAAGTGCC GCCAACAACA GCAACAACAG
CAGAATGGAG GTCAAAACAA AGTGAGACCT GCCAAAAAGA AGACATCTCC AGCTCGGGAA
GTGAGTTCAG AGAGTGGAAC AAGTGGCCAA TTCACTCCCC CCTCTAGCAC CTCAGTCCCG
ACCATTGCCA GCAGCAGTGC TCCTGTGTCT ATCTGGAGCC CAGCTTCCAT CTCCCCACTG
TCAGATCCCT TGTCCACCTC CTCTTCCTGC ATGCAGAGGT CCTATCCCAT GACCTATACT
CAGGCTTCAG GTTATAGTCA AGGATATGCT GGCTCAACTT CCTACTTTGG GGGCATGGAC
TGTGGATCAT ATTTGACCCC TATGCATCAC CAGCTTCCCG GACCAGGGGC CACACTCAGT
CCCATGGGTA CCAATGCAGT CACCAGCCAT CTCAATCAGT CCCCAGCTTC TCTTTCCACC
CAGGGATATG GAGCTTCAAG CTTGGGTTTT AACTCAACCA CTGATTGCTT GGATTATAAG
GACCAAACTG CCTCCTGGAA GCTTAACTTC AATGCTGACT GCTTGGATTA TAAAGATCAG
ACATCCTCGT GGAAATTCCA GGTTTTGTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_758840.1
CDS208..1077
Misc Feature(1)49..51(+)
Misc Feature(2)322..489(+)
Misc Feature(3)322..489(+)
Misc Feature(4)328..480(+)
Misc Feature(5)664..912(+)
Exon (1)1..304
Gene:OTX2
Gene Synonym:
Exon (2)305..456
Gene:OTX2
Gene Synonym:
Exon (3)457..2105
Gene:OTX2
Gene Synonym:
Translation
Position Chain Variation Link
116 116 c, g dbSNP:566671671
126 126 c, g dbSNP:540831263
157 157 a, t dbSNP:546858272
158 158 a, g dbSNP:747816534
161 161 c, g dbSNP:776104541
162 162 a, g dbSNP:200002729
164 164 c, t dbSNP:373972732
165 165 a, g dbSNP:564618315
167 167 c, t dbSNP:779606022
170 170 c, t dbSNP:758339288
175 175 c, t dbSNP:546311189
179 179 a, c dbSNP:370535121
181 181 c, g dbSNP:757069643
187 187 c, g dbSNP:754146678
191 191 c, t dbSNP:764157150
199 199 a, g dbSNP:756345000
201 201 a, c dbSNP:752785578
211 211 a, g dbSNP:767603643
216 216 c, t dbSNP:575736431
222 222 g, t dbSNP:752010967
232 232 c, t dbSNP:766756721
238 238 g, t dbSNP:763234678
245 245 a, g dbSNP:776356711
246 246 c, t dbSNP:768254721
249 249 g, t dbSNP:760374061
264 264 c, t dbSNP:774907916
265 265 c, t dbSNP:771687556
269 269 a, g dbSNP:745822138
270 270 -, t dbSNP:779418668
271 271 a, g dbSNP:200680654
273 273 c, g dbSNP:778956556
276 276 a, c dbSNP:770855748
283 283 c, g dbSNP:749151724
288 288 -, c dbSNP:786205874
292 292 a, g dbSNP:777618282
296 296 a, g dbSNP:756331034
302 302 c, t dbSNP:752941534
308 308 a, c dbSNP:758946986
309 309 c, t dbSNP:750923926
310 310 c, g dbSNP:374042850
313 313 -, c dbSNP:758119168
345 345 c, t dbSNP:757602259
359 359 a, g dbSNP:567955379
370 370 g, t dbSNP:368759331
375 375 a, g dbSNP:141664597
386 386 c, t dbSNP:759256101
387 387 c, t dbSNP:774043425
390 390 a, g dbSNP:765964221
396 396 a, g dbSNP:762821214
409 409 a, c dbSNP:773157352
441 441 c, t dbSNP:769477143
442 442 a, g dbSNP:786205224
447 447 a, g dbSNP:376651816
453 453 g, t dbSNP:747916036
472 472 c, g dbSNP:104894464
473 473 a, g dbSNP:200990681
490 490 a, c dbSNP:78953717
492 492 a, g dbSNP:749788509
499 499 -, c dbSNP:786205884
513 513 c, t dbSNP:778304481
519 519 c, t dbSNP:536495343
524 524 a, g dbSNP:751258203
533 533 c, g dbSNP:779696471
534 534 a, c dbSNP:757971368
535 535 a, c dbSNP:750013761
545 545 a, g dbSNP:764625033
549 549 a, g dbSNP:761862286
554 554 c, t dbSNP:753729345
555 555 c, t dbSNP:763911822
556 556 c, t dbSNP:760396081
562 562 c, t dbSNP:775702810
563 563 a, c, g dbSNP:199799627
569 569 g, t dbSNP:774537529
571 571 a, g dbSNP:748205899
579 579 a, g dbSNP:749843466
584 584 c, g dbSNP:778357805
589 589 a, g dbSNP:770291855
594 594 c, t dbSNP:772021946
595 595 c, t dbSNP:74658806
601 601 a, c, g dbSNP:758106958
604 604 a, c dbSNP:376333965
606 606 a, c, t dbSNP:372008404
607 607 c, g dbSNP:753783256
608 608 a, c, g dbSNP:199761861
609 609 c, t dbSNP:553884027
620 620 c, g dbSNP:786205879
626 626 c, t dbSNP:201721391
627 627 a, c, g dbSNP:147896150
629 629 c, t dbSNP:771047348
630 630 c, t dbSNP:763064579
634 634 a, g, t dbSNP:770486100
635 635 c, t dbSNP:748631596
636 636 c, t dbSNP:777104497
638 638 a, g dbSNP:768857621
642 642 c, t dbSNP:34537598
647 647 c, g, t dbSNP:200171280
660 660 a, c dbSNP:373798705
666 666 c, t dbSNP:538192166
671 671 -, gc dbSNP:786205873
677 677 a, t dbSNP:756087368
687 687 g, t dbSNP:752526255
697 697 a, t dbSNP:370354056
708 708 c, t dbSNP:767374937
723 723 a, g dbSNP:368033398
733 733 c, t dbSNP:751820068
735 735 a, c dbSNP:766762080
744 744 a, c, t dbSNP:104894465
757 757 g, t dbSNP:773463323
763 763 a, g dbSNP:765382721
764 764 a, g dbSNP:762435469
767 767 a, g dbSNP:776964451
769 769 g, t dbSNP:397514463
770 770 g, t dbSNP:142205965
774 774 c, t dbSNP:747279627
780 780 c, g dbSNP:775913832
784 784 a, g, t dbSNP:748910557
785 785 a, c, t dbSNP:755578825
824 824 a, c dbSNP:150982073
826 826 a, c dbSNP:780929725
838 838 c, g dbSNP:754929157
839 839 a, c dbSNP:751376563
844 844 c, t dbSNP:548455339
846 846 c, t dbSNP:142743327
847 847 a, g dbSNP:374968145
856 856 a, g dbSNP:750626422
860 860 c, t dbSNP:765441049
863 863 g, t dbSNP:761733794
865 865 a, g dbSNP:529946021
868 868 -, cccatgggt dbSNP:745480462
876 876 c, t dbSNP:764571884
881 881 a, g dbSNP:370761964
888 888 c, t dbSNP:775876318
896 896 a, g, t dbSNP:144449264
898 898 c, t dbSNP:772782540
907 907 c, t dbSNP:769304167
914 914 c, t dbSNP:747665958
922 922 c, t dbSNP:143131956
926 926 c, t dbSNP:199650198
931 931 c, g dbSNP:768514532
932 932 c, g dbSNP:746941491
940 940 a, g dbSNP:139800030
941 941 c, t dbSNP:758228717
947 947 a, g dbSNP:781096873
965 965 c, t dbSNP:750679604
980 980 a, c dbSNP:779220317
1014 1014 c, t dbSNP:78559885
1023 1023 c, t dbSNP:201396096
1027 1027 a, t dbSNP:764205900
1049 1049 c, t dbSNP:761138937
1055 1055 c, t dbSNP:753156713
1056 1056 a, c, g dbSNP:759833842
1064 1064 c, t dbSNP:774680300
1079 1079 a, g dbSNP:370275924
1082 1082 a, c, t dbSNP:776053080
1087 1087 a, g dbSNP:171978
1103 1103 a, c, g dbSNP:780072062
1107 1107 c, t dbSNP:772006682
1111 1111 a, t dbSNP:182392988
1118 1118 a, g dbSNP:3742576
1120 1120 g, t dbSNP:558072316
1123 1123 a, g dbSNP:757464106
1198 1198 a, g dbSNP:575988911
1201 1201 c, t dbSNP:555242082
1202 1202 c, t dbSNP:561466216
1203 1203 a, c, g dbSNP:750146313
1223 1223 c, t dbSNP:543291377
1237 1237 a, g dbSNP:75947588
1240 1240 a, g dbSNP:764880954
1338 1338 c, g dbSNP:553969803
1350 1350 c, t dbSNP:538805998
1393 1393 a, g dbSNP:142727455
1416 1416 c, t dbSNP:556614831
1417 1417 -, c dbSNP:3215889
1422 1422 a, c dbSNP:537889967
1436 1436 g, t dbSNP:567634820
1464 1464 g, t dbSNP:372333803
1501 1501 a, c dbSNP:778705827
1504 1504 a, g dbSNP:761387465
1513 1513 c, g dbSNP:190556214
1568 1568 a, g dbSNP:369779296
1569 1569 -, attg dbSNP:149455658
1586 1586 a, g dbSNP:754230866
1672 1672 a, g dbSNP:764392879
1710 1710 c, t dbSNP:536571793
1744 1744 a, c dbSNP:184421963
1754 1754 c, t dbSNP:56245644
1775 1775 a, t dbSNP:58246365
1799 1799 a, g dbSNP:192422162
1841 1841 a, g dbSNP:775857186
1856 1856 a, g dbSNP:138197536
1899 1899 a, t dbSNP:187055500
1905 1905 a, g dbSNP:772253161
1935 1935 a, g dbSNP:549838567
1996 1996 c, g dbSNP:74517617
1997 1997 a, g dbSNP:80140046
1998 1998 a, t dbSNP:78935901
1999 1999 c, t dbSNP:759551235
2002 2002 c, t dbSNP:781146554
2035 2035 a, g dbSNP:767628237
2043 2043 a, g dbSNP:57390094
2085 2085 a, g dbSNP:184224534

Target ORF information:

RefSeq Version NM_172337
Organism Homo sapiens (human)
Definition Homo sapiens orthodenticle homeobox 2 (OTX2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_172337

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGATGTCTT ATCTTAAGCA ACCGCCTTAC GCAGTCAATG GGCTGAGTCT GACCACTTCG 
GGTATGGACT TGCTGCACCC CTCCGTGGGC TACCCGGCCA CCCCCCGGAA ACAGCGCCGG
GAGAGGACGA CGTTCACTCG GGCGCAGCTA GATGTGCTGG AAGCACTGTT TGCCAAGACC
CGGTACCCAG ACATCTTCAT GCGAGAGGAG GTGGCACTGA AAATCAACTT GCCCGAGTCG
AGGGTGCAGG TATGGTTTAA GAATCGAAGA GCTAAGTGCC GCCAACAACA GCAACAACAG
CAGAATGGAG GTCAAAACAA AGTGAGACCT GCCAAAAAGA AGACATCTCC AGCTCGGGAA
GTGAGTTCAG AGAGTGGAAC AAGTGGCCAA TTCACTCCCC CCTCTAGCAC CTCAGTCCCG
ACCATTGCCA GCAGCAGTGC TCCTGTGTCT ATCTGGAGCC CAGCTTCCAT CTCCCCACTG
TCAGATCCCT TGTCCACCTC CTCTTCCTGC ATGCAGAGGT CCTATCCCAT GACCTATACT
CAGGCTTCAG GTTATAGTCA AGGATATGCT GGCTCAACTT CCTACTTTGG GGGCATGGAC
TGTGGATCAT ATTTGACCCC TATGCATCAC CAGCTTCCCG GACCAGGGGC CACACTCAGT
CCCATGGGTA CCAATGCAGT CACCAGCCAT CTCAATCAGT CCCCAGCTTC TCTTTCCACC
CAGGGATATG GAGCTTCAAG CTTGGGTTTT AACTCAACCA CTGATTGCTT GGATTATAAG
GACCAAACTG CCTCCTGGAA GCTTAACTTC AATGCTGACT GCTTGGATTA TAAAGATCAG
ACATCCTCGT GGAAATTCCA GGTTTTGTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu16931D
Sequence Information ORF Nucleotide Sequence (Length: 870bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product homeobox protein OTX2 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB593058.1, CV812961.1 and BC032579.1. Summary: This gene encodes a member of the bicoid subfamily of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and plays a role in brain, craniofacial, and sensory organ development. The encoded protein also influences the proliferation and differentiation of dopaminergic neuronal progenitor cells during mitosis. Mutations in this gene cause syndromic microphthalmia 5 (MCOPS5) and combined pituitary hormone deficiency 6 (CPHD6). This gene is also suspected of having an oncogenic role in medulloblastoma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Pseudogenes of this gene are known to exist on chromosomes two and nine. [provided by RefSeq, Jul 2012]. Transcript Variant: This variant (3) lacks an in-frame segment of the coding region, compared to variant 1, and, compared to isoform a, encodes a shorter isoform (b). Variants 2-4 encode the same protein (isoform b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CV812961.1, BY795606.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962344 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGATGTCTT ATCTTAAGCA ACCGCCTTAC GCAGTCAATG GGCTGAGTCT GACCACTTCG 
GGTATGGACT TGCTGCACCC CTCCGTGGGC TACCCGGCCA CCCCCCGGAA ACAGCGCCGG
GAGAGGACGA CGTTCACTCG GGCGCAGCTA GATGTGCTGG AAGCACTGTT TGCCAAGACC
CGGTACCCAG ACATCTTCAT GCGAGAGGAG GTGGCACTGA AAATCAACTT GCCCGAGTCG
AGGGTGCAGG TATGGTTTAA GAATCGAAGA GCTAAGTGCC GCCAACAACA GCAACAACAG
CAGAATGGAG GTCAAAACAA AGTGAGACCT GCCAAAAAGA AGACATCTCC AGCTCGGGAA
GTGAGTTCAG AGAGTGGAAC AAGTGGCCAA TTCACTCCCC CCTCTAGCAC CTCAGTCCCG
ACCATTGCCA GCAGCAGTGC TCCTGTGTCT ATCTGGAGCC CAGCTTCCAT CTCCCCACTG
TCAGATCCCT TGTCCACCTC CTCTTCCTGC ATGCAGAGGT CCTATCCCAT GACCTATACT
CAGGCTTCAG GTTATAGTCA AGGATATGCT GGCTCAACTT CCTACTTTGG GGGCATGGAC
TGTGGATCAT ATTTGACCCC TATGCATCAC CAGCTTCCCG GACCAGGGGC CACACTCAGT
CCCATGGGTA CCAATGCAGT CACCAGCCAT CTCAATCAGT CCCCAGCTTC TCTTTCCACC
CAGGGATATG GAGCTTCAAG CTTGGGTTTT AACTCAACCA CTGATTGCTT GGATTATAAG
GACCAAACTG CCTCCTGGAA GCTTAACTTC AATGCTGACT GCTTGGATTA TAAAGATCAG
ACATCCTCGT GGAAATTCCA GGTTTTGTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001257452.1
CDS285..1154
Misc Feature(1)162..164(+)
Misc Feature(2)399..566(+)
Misc Feature(3)399..566(+)
Misc Feature(4)405..557(+)
Misc Feature(5)741..989(+)
Exon (1)1..101
Gene:OTX2
Gene Synonym:
Exon (2)102..165
Gene:OTX2
Gene Synonym:
Exon (3)166..381
Gene:OTX2
Gene Synonym:
Exon (4)382..533
Gene:OTX2
Gene Synonym:
Exon (5)534..2182
Gene:OTX2
Gene Synonym:
Translation
Position Chain Variation Link
22 22 c, g dbSNP:563987229
23 23 a, g dbSNP:746287642
80 80 c, t dbSNP:535146235
81 81 c, t dbSNP:566447220
88 88 g, t dbSNP:200705423
92 92 c, t dbSNP:773133418
97 97 a, g dbSNP:138281551
98 98 c, t dbSNP:185374570
111 111 c, t dbSNP:150887670
158 158 g, t dbSNP:780689107
193 193 c, g dbSNP:566671671
203 203 c, g dbSNP:540831263
234 234 a, t dbSNP:546858272
235 235 a, g dbSNP:747816534
238 238 c, g dbSNP:776104541
239 239 a, g dbSNP:200002729
241 241 c, t dbSNP:373972732
242 242 a, g dbSNP:564618315
244 244 c, t dbSNP:779606022
247 247 c, t dbSNP:758339288
252 252 c, t dbSNP:546311189
256 256 a, c dbSNP:370535121
258 258 c, g dbSNP:757069643
264 264 c, g dbSNP:754146678
268 268 c, t dbSNP:764157150
276 276 a, g dbSNP:756345000
278 278 a, c dbSNP:752785578
288 288 a, g dbSNP:767603643
293 293 c, t dbSNP:575736431
299 299 g, t dbSNP:752010967
309 309 c, t dbSNP:766756721
315 315 g, t dbSNP:763234678
322 322 a, g dbSNP:776356711
323 323 c, t dbSNP:768254721
326 326 g, t dbSNP:760374061
341 341 c, t dbSNP:774907916
342 342 c, t dbSNP:771687556
346 346 a, g dbSNP:745822138
347 347 -, t dbSNP:779418668
348 348 a, g dbSNP:200680654
350 350 c, g dbSNP:778956556
353 353 a, c dbSNP:770855748
360 360 c, g dbSNP:749151724
365 365 -, c dbSNP:786205874
369 369 a, g dbSNP:777618282
373 373 a, g dbSNP:756331034
379 379 c, t dbSNP:752941534
385 385 a, c dbSNP:758946986
386 386 c, t dbSNP:750923926
387 387 c, g dbSNP:374042850
390 390 -, c dbSNP:758119168
422 422 c, t dbSNP:757602259
436 436 a, g dbSNP:567955379
447 447 g, t dbSNP:368759331
452 452 a, g dbSNP:141664597
463 463 c, t dbSNP:759256101
464 464 c, t dbSNP:774043425
467 467 a, g dbSNP:765964221
473 473 a, g dbSNP:762821214
486 486 a, c dbSNP:773157352
518 518 c, t dbSNP:769477143
519 519 a, g dbSNP:786205224
524 524 a, g dbSNP:376651816
530 530 g, t dbSNP:747916036
549 549 c, g dbSNP:104894464
550 550 a, g dbSNP:200990681
567 567 a, c dbSNP:78953717
569 569 a, g dbSNP:749788509
576 576 -, c dbSNP:786205884
590 590 c, t dbSNP:778304481
596 596 c, t dbSNP:536495343
601 601 a, g dbSNP:751258203
610 610 c, g dbSNP:779696471
611 611 a, c dbSNP:757971368
612 612 a, c dbSNP:750013761
622 622 a, g dbSNP:764625033
626 626 a, g dbSNP:761862286
631 631 c, t dbSNP:753729345
632 632 c, t dbSNP:763911822
633 633 c, t dbSNP:760396081
639 639 c, t dbSNP:775702810
640 640 a, c, g dbSNP:199799627
646 646 g, t dbSNP:774537529
648 648 a, g dbSNP:748205899
656 656 a, g dbSNP:749843466
661 661 c, g dbSNP:778357805
666 666 a, g dbSNP:770291855
671 671 c, t dbSNP:772021946
672 672 c, t dbSNP:74658806
678 678 a, c, g dbSNP:758106958
681 681 a, c dbSNP:376333965
683 683 a, c, t dbSNP:372008404
684 684 c, g dbSNP:753783256
685 685 a, c, g dbSNP:199761861
686 686 c, t dbSNP:553884027
697 697 c, g dbSNP:786205879
703 703 c, t dbSNP:201721391
704 704 a, c, g dbSNP:147896150
706 706 c, t dbSNP:771047348
707 707 c, t dbSNP:763064579
711 711 a, g, t dbSNP:770486100
712 712 c, t dbSNP:748631596
713 713 c, t dbSNP:777104497
715 715 a, g dbSNP:768857621
719 719 c, t dbSNP:34537598
724 724 c, g, t dbSNP:200171280
737 737 a, c dbSNP:373798705
743 743 c, t dbSNP:538192166
748 748 -, gc dbSNP:786205873
754 754 a, t dbSNP:756087368
764 764 g, t dbSNP:752526255
774 774 a, t dbSNP:370354056
785 785 c, t dbSNP:767374937
800 800 a, g dbSNP:368033398
810 810 c, t dbSNP:751820068
812 812 a, c dbSNP:766762080
821 821 a, c, t dbSNP:104894465
834 834 g, t dbSNP:773463323
840 840 a, g dbSNP:765382721
841 841 a, g dbSNP:762435469
844 844 a, g dbSNP:776964451
846 846 g, t dbSNP:397514463
847 847 g, t dbSNP:142205965
851 851 c, t dbSNP:747279627
857 857 c, g dbSNP:775913832
861 861 a, g, t dbSNP:748910557
862 862 a, c, t dbSNP:755578825
901 901 a, c dbSNP:150982073
903 903 a, c dbSNP:780929725
915 915 c, g dbSNP:754929157
916 916 a, c dbSNP:751376563
921 921 c, t dbSNP:548455339
923 923 c, t dbSNP:142743327
924 924 a, g dbSNP:374968145
933 933 a, g dbSNP:750626422
937 937 c, t dbSNP:765441049
940 940 g, t dbSNP:761733794
942 942 a, g dbSNP:529946021
945 945 -, cccatgggt dbSNP:745480462
953 953 c, t dbSNP:764571884
958 958 a, g dbSNP:370761964
965 965 c, t dbSNP:775876318
973 973 a, g, t dbSNP:144449264
975 975 c, t dbSNP:772782540
984 984 c, t dbSNP:769304167
991 991 c, t dbSNP:747665958
999 999 c, t dbSNP:143131956
1003 1003 c, t dbSNP:199650198
1008 1008 c, g dbSNP:768514532
1009 1009 c, g dbSNP:746941491
1017 1017 a, g dbSNP:139800030
1018 1018 c, t dbSNP:758228717
1024 1024 a, g dbSNP:781096873
1042 1042 c, t dbSNP:750679604
1057 1057 a, c dbSNP:779220317
1091 1091 c, t dbSNP:78559885
1100 1100 c, t dbSNP:201396096
1104 1104 a, t dbSNP:764205900
1126 1126 c, t dbSNP:761138937
1132 1132 c, t dbSNP:753156713
1133 1133 a, c, g dbSNP:759833842
1141 1141 c, t dbSNP:774680300
1156 1156 a, g dbSNP:370275924
1159 1159 a, c, t dbSNP:776053080
1164 1164 a, g dbSNP:171978
1180 1180 a, c, g dbSNP:780072062
1184 1184 c, t dbSNP:772006682
1188 1188 a, t dbSNP:182392988
1195 1195 a, g dbSNP:3742576
1197 1197 g, t dbSNP:558072316
1200 1200 a, g dbSNP:757464106
1275 1275 a, g dbSNP:575988911
1278 1278 c, t dbSNP:555242082
1279 1279 c, t dbSNP:561466216
1280 1280 a, c, g dbSNP:750146313
1300 1300 c, t dbSNP:543291377
1314 1314 a, g dbSNP:75947588
1317 1317 a, g dbSNP:764880954
1415 1415 c, g dbSNP:553969803
1427 1427 c, t dbSNP:538805998
1470 1470 a, g dbSNP:142727455
1493 1493 c, t dbSNP:556614831
1494 1494 -, c dbSNP:3215889
1499 1499 a, c dbSNP:537889967
1513 1513 g, t dbSNP:567634820
1541 1541 g, t dbSNP:372333803
1578 1578 a, c dbSNP:778705827
1581 1581 a, g dbSNP:761387465
1590 1590 c, g dbSNP:190556214
1645 1645 a, g dbSNP:369779296
1646 1646 -, attg dbSNP:149455658
1663 1663 a, g dbSNP:754230866
1749 1749 a, g dbSNP:764392879
1787 1787 c, t dbSNP:536571793
1821 1821 a, c dbSNP:184421963
1831 1831 c, t dbSNP:56245644
1852 1852 a, t dbSNP:58246365
1876 1876 a, g dbSNP:192422162
1918 1918 a, g dbSNP:775857186
1933 1933 a, g dbSNP:138197536
1976 1976 a, t dbSNP:187055500
1982 1982 a, g dbSNP:772253161
2012 2012 a, g dbSNP:549838567
2073 2073 c, g dbSNP:74517617
2074 2074 a, g dbSNP:80140046
2075 2075 a, t dbSNP:78935901
2076 2076 c, t dbSNP:759551235
2079 2079 c, t dbSNP:781146554
2112 2112 a, g dbSNP:767628237
2120 2120 a, g dbSNP:57390094
2162 2162 a, g dbSNP:184224534

Target ORF information:

RefSeq Version NM_001270523
Organism Homo sapiens (human)
Definition Homo sapiens orthodenticle homeobox 2 (OTX2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001270523

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGATGTCTT ATCTTAAGCA ACCGCCTTAC GCAGTCAATG GGCTGAGTCT GACCACTTCG 
GGTATGGACT TGCTGCACCC CTCCGTGGGC TACCCGGCCA CCCCCCGGAA ACAGCGCCGG
GAGAGGACGA CGTTCACTCG GGCGCAGCTA GATGTGCTGG AAGCACTGTT TGCCAAGACC
CGGTACCCAG ACATCTTCAT GCGAGAGGAG GTGGCACTGA AAATCAACTT GCCCGAGTCG
AGGGTGCAGG TATGGTTTAA GAATCGAAGA GCTAAGTGCC GCCAACAACA GCAACAACAG
CAGAATGGAG GTCAAAACAA AGTGAGACCT GCCAAAAAGA AGACATCTCC AGCTCGGGAA
GTGAGTTCAG AGAGTGGAAC AAGTGGCCAA TTCACTCCCC CCTCTAGCAC CTCAGTCCCG
ACCATTGCCA GCAGCAGTGC TCCTGTGTCT ATCTGGAGCC CAGCTTCCAT CTCCCCACTG
TCAGATCCCT TGTCCACCTC CTCTTCCTGC ATGCAGAGGT CCTATCCCAT GACCTATACT
CAGGCTTCAG GTTATAGTCA AGGATATGCT GGCTCAACTT CCTACTTTGG GGGCATGGAC
TGTGGATCAT ATTTGACCCC TATGCATCAC CAGCTTCCCG GACCAGGGGC CACACTCAGT
CCCATGGGTA CCAATGCAGT CACCAGCCAT CTCAATCAGT CCCCAGCTTC TCTTTCCACC
CAGGGATATG GAGCTTCAAG CTTGGGTTTT AACTCAACCA CTGATTGCTT GGATTATAAG
GACCAAACTG CCTCCTGGAA GCTTAACTTC AATGCTGACT GCTTGGATTA TAAAGATCAG
ACATCCTCGT GGAAATTCCA GGTTTTGTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu16931D
Sequence Information ORF Nucleotide Sequence (Length: 870bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product homeobox protein OTX2 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB593058.1, AK314271.1 and BC032579.1. Summary: This gene encodes a member of the bicoid subfamily of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and plays a role in brain, craniofacial, and sensory organ development. The encoded protein also influences the proliferation and differentiation of dopaminergic neuronal progenitor cells during mitosis. Mutations in this gene cause syndromic microphthalmia 5 (MCOPS5) and combined pituitary hormone deficiency 6 (CPHD6). This gene is also suspected of having an oncogenic role in medulloblastoma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Pseudogenes of this gene are known to exist on chromosomes two and nine. [provided by RefSeq, Jul 2012]. Transcript Variant: This variant (4) differs in the 5' UTR and lacks an in-frame segment of the coding region, compared to variant 1, and, compared to isoform a, encodes a shorter isoform (b). Variants 2-4 encode the same protein (isoform b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314271.1, BM564760.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962344 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGATGTCTT ATCTTAAGCA ACCGCCTTAC GCAGTCAATG GGCTGAGTCT GACCACTTCG 
GGTATGGACT TGCTGCACCC CTCCGTGGGC TACCCGGCCA CCCCCCGGAA ACAGCGCCGG
GAGAGGACGA CGTTCACTCG GGCGCAGCTA GATGTGCTGG AAGCACTGTT TGCCAAGACC
CGGTACCCAG ACATCTTCAT GCGAGAGGAG GTGGCACTGA AAATCAACTT GCCCGAGTCG
AGGGTGCAGG TATGGTTTAA GAATCGAAGA GCTAAGTGCC GCCAACAACA GCAACAACAG
CAGAATGGAG GTCAAAACAA AGTGAGACCT GCCAAAAAGA AGACATCTCC AGCTCGGGAA
GTGAGTTCAG AGAGTGGAAC AAGTGGCCAA TTCACTCCCC CCTCTAGCAC CTCAGTCCCG
ACCATTGCCA GCAGCAGTGC TCCTGTGTCT ATCTGGAGCC CAGCTTCCAT CTCCCCACTG
TCAGATCCCT TGTCCACCTC CTCTTCCTGC ATGCAGAGGT CCTATCCCAT GACCTATACT
CAGGCTTCAG GTTATAGTCA AGGATATGCT GGCTCAACTT CCTACTTTGG GGGCATGGAC
TGTGGATCAT ATTTGACCCC TATGCATCAC CAGCTTCCCG GACCAGGGGC CACACTCAGT
CCCATGGGTA CCAATGCAGT CACCAGCCAT CTCAATCAGT CCCCAGCTTC TCTTTCCACC
CAGGGATATG GAGCTTCAAG CTTGGGTTTT AACTCAACCA CTGATTGCTT GGATTATAAG
GACCAAACTG CCTCCTGGAA GCTTAACTTC AATGCTGACT GCTTGGATTA TAAAGATCAG
ACATCCTCGT GGAAATTCCA GGTTTTGTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001257453.1
CDS438..1307
Misc Feature(1)315..317(+)
Misc Feature(2)552..719(+)
Misc Feature(3)552..719(+)
Misc Feature(4)558..710(+)
Misc Feature(5)894..1142(+)
Exon (1)1..318
Gene:OTX2
Gene Synonym:
Exon (2)319..534
Gene:OTX2
Gene Synonym:
Exon (3)535..686
Gene:OTX2
Gene Synonym:
Exon (4)687..2335
Gene:OTX2
Gene Synonym:
Translation
Position Chain Variation Link
22 22 c, g dbSNP:563987229
23 23 a, g dbSNP:746287642
80 80 c, t dbSNP:535146235
81 81 c, t dbSNP:566447220
88 88 g, t dbSNP:200705423
92 92 c, t dbSNP:773133418
97 97 a, g dbSNP:138281551
98 98 c, t dbSNP:185374570
115 115 -, a dbSNP:570520714
117 117 a, g dbSNP:541127366
127 127 g, t dbSNP:192478552
171 171 a, c dbSNP:571695883
174 174 a, t dbSNP:12590685
176 176 g, t dbSNP:559258863
195 195 c, g dbSNP:550599732
264 264 c, t dbSNP:150887670
311 311 g, t dbSNP:780689107
346 346 c, g dbSNP:566671671
356 356 c, g dbSNP:540831263
387 387 a, t dbSNP:546858272
388 388 a, g dbSNP:747816534
391 391 c, g dbSNP:776104541
392 392 a, g dbSNP:200002729
394 394 c, t dbSNP:373972732
395 395 a, g dbSNP:564618315
397 397 c, t dbSNP:779606022
400 400 c, t dbSNP:758339288
405 405 c, t dbSNP:546311189
409 409 a, c dbSNP:370535121
411 411 c, g dbSNP:757069643
417 417 c, g dbSNP:754146678
421 421 c, t dbSNP:764157150
429 429 a, g dbSNP:756345000
431 431 a, c dbSNP:752785578
441 441 a, g dbSNP:767603643
446 446 c, t dbSNP:575736431
452 452 g, t dbSNP:752010967
462 462 c, t dbSNP:766756721
468 468 g, t dbSNP:763234678
475 475 a, g dbSNP:776356711
476 476 c, t dbSNP:768254721
479 479 g, t dbSNP:760374061
494 494 c, t dbSNP:774907916
495 495 c, t dbSNP:771687556
499 499 a, g dbSNP:745822138
500 500 -, t dbSNP:779418668
501 501 a, g dbSNP:200680654
503 503 c, g dbSNP:778956556
506 506 a, c dbSNP:770855748
513 513 c, g dbSNP:749151724
518 518 -, c dbSNP:786205874
522 522 a, g dbSNP:777618282
526 526 a, g dbSNP:756331034
532 532 c, t dbSNP:752941534
538 538 a, c dbSNP:758946986
539 539 c, t dbSNP:750923926
540 540 c, g dbSNP:374042850
543 543 -, c dbSNP:758119168
575 575 c, t dbSNP:757602259
589 589 a, g dbSNP:567955379
600 600 g, t dbSNP:368759331
605 605 a, g dbSNP:141664597
616 616 c, t dbSNP:759256101
617 617 c, t dbSNP:774043425
620 620 a, g dbSNP:765964221
626 626 a, g dbSNP:762821214
639 639 a, c dbSNP:773157352
671 671 c, t dbSNP:769477143
672 672 a, g dbSNP:786205224
677 677 a, g dbSNP:376651816
683 683 g, t dbSNP:747916036
702 702 c, g dbSNP:104894464
703 703 a, g dbSNP:200990681
720 720 a, c dbSNP:78953717
722 722 a, g dbSNP:749788509
729 729 -, c dbSNP:786205884
743 743 c, t dbSNP:778304481
749 749 c, t dbSNP:536495343
754 754 a, g dbSNP:751258203
763 763 c, g dbSNP:779696471
764 764 a, c dbSNP:757971368
765 765 a, c dbSNP:750013761
775 775 a, g dbSNP:764625033
779 779 a, g dbSNP:761862286
784 784 c, t dbSNP:753729345
785 785 c, t dbSNP:763911822
786 786 c, t dbSNP:760396081
792 792 c, t dbSNP:775702810
793 793 a, c, g dbSNP:199799627
799 799 g, t dbSNP:774537529
801 801 a, g dbSNP:748205899
809 809 a, g dbSNP:749843466
814 814 c, g dbSNP:778357805
819 819 a, g dbSNP:770291855
824 824 c, t dbSNP:772021946
825 825 c, t dbSNP:74658806
831 831 a, c, g dbSNP:758106958
834 834 a, c dbSNP:376333965
836 836 a, c, t dbSNP:372008404
837 837 c, g dbSNP:753783256
838 838 a, c, g dbSNP:199761861
839 839 c, t dbSNP:553884027
850 850 c, g dbSNP:786205879
856 856 c, t dbSNP:201721391
857 857 a, c, g dbSNP:147896150
859 859 c, t dbSNP:771047348
860 860 c, t dbSNP:763064579
864 864 a, g, t dbSNP:770486100
865 865 c, t dbSNP:748631596
866 866 c, t dbSNP:777104497
868 868 a, g dbSNP:768857621
872 872 c, t dbSNP:34537598
877 877 c, g, t dbSNP:200171280
890 890 a, c dbSNP:373798705
896 896 c, t dbSNP:538192166
901 901 -, gc dbSNP:786205873
907 907 a, t dbSNP:756087368
917 917 g, t dbSNP:752526255
927 927 a, t dbSNP:370354056
938 938 c, t dbSNP:767374937
953 953 a, g dbSNP:368033398
963 963 c, t dbSNP:751820068
965 965 a, c dbSNP:766762080
974 974 a, c, t dbSNP:104894465
987 987 g, t dbSNP:773463323
993 993 a, g dbSNP:765382721
994 994 a, g dbSNP:762435469
997 997 a, g dbSNP:776964451
999 999 g, t dbSNP:397514463
1000 1000 g, t dbSNP:142205965
1004 1004 c, t dbSNP:747279627
1010 1010 c, g dbSNP:775913832
1014 1014 a, g, t dbSNP:748910557
1015 1015 a, c, t dbSNP:755578825
1054 1054 a, c dbSNP:150982073
1056 1056 a, c dbSNP:780929725
1068 1068 c, g dbSNP:754929157
1069 1069 a, c dbSNP:751376563
1074 1074 c, t dbSNP:548455339
1076 1076 c, t dbSNP:142743327
1077 1077 a, g dbSNP:374968145
1086 1086 a, g dbSNP:750626422
1090 1090 c, t dbSNP:765441049
1093 1093 g, t dbSNP:761733794
1095 1095 a, g dbSNP:529946021
1098 1098 -, cccatgggt dbSNP:745480462
1106 1106 c, t dbSNP:764571884
1111 1111 a, g dbSNP:370761964
1118 1118 c, t dbSNP:775876318
1126 1126 a, g, t dbSNP:144449264
1128 1128 c, t dbSNP:772782540
1137 1137 c, t dbSNP:769304167
1144 1144 c, t dbSNP:747665958
1152 1152 c, t dbSNP:143131956
1156 1156 c, t dbSNP:199650198
1161 1161 c, g dbSNP:768514532
1162 1162 c, g dbSNP:746941491
1170 1170 a, g dbSNP:139800030
1171 1171 c, t dbSNP:758228717
1177 1177 a, g dbSNP:781096873
1195 1195 c, t dbSNP:750679604
1210 1210 a, c dbSNP:779220317
1244 1244 c, t dbSNP:78559885
1253 1253 c, t dbSNP:201396096
1257 1257 a, t dbSNP:764205900
1279 1279 c, t dbSNP:761138937
1285 1285 c, t dbSNP:753156713
1286 1286 a, c, g dbSNP:759833842
1294 1294 c, t dbSNP:774680300
1309 1309 a, g dbSNP:370275924
1312 1312 a, c, t dbSNP:776053080
1317 1317 a, g dbSNP:171978
1333 1333 a, c, g dbSNP:780072062
1337 1337 c, t dbSNP:772006682
1341 1341 a, t dbSNP:182392988
1348 1348 a, g dbSNP:3742576
1350 1350 g, t dbSNP:558072316
1353 1353 a, g dbSNP:757464106
1428 1428 a, g dbSNP:575988911
1431 1431 c, t dbSNP:555242082
1432 1432 c, t dbSNP:561466216
1433 1433 a, c, g dbSNP:750146313
1453 1453 c, t dbSNP:543291377
1467 1467 a, g dbSNP:75947588
1470 1470 a, g dbSNP:764880954
1568 1568 c, g dbSNP:553969803
1580 1580 c, t dbSNP:538805998
1623 1623 a, g dbSNP:142727455
1646 1646 c, t dbSNP:556614831
1647 1647 -, c dbSNP:3215889
1652 1652 a, c dbSNP:537889967
1666 1666 g, t dbSNP:567634820
1694 1694 g, t dbSNP:372333803
1731 1731 a, c dbSNP:778705827
1734 1734 a, g dbSNP:761387465
1743 1743 c, g dbSNP:190556214
1798 1798 a, g dbSNP:369779296
1799 1799 -, attg dbSNP:149455658
1816 1816 a, g dbSNP:754230866
1902 1902 a, g dbSNP:764392879
1940 1940 c, t dbSNP:536571793
1974 1974 a, c dbSNP:184421963
1984 1984 c, t dbSNP:56245644
2005 2005 a, t dbSNP:58246365
2029 2029 a, g dbSNP:192422162
2071 2071 a, g dbSNP:775857186
2086 2086 a, g dbSNP:138197536
2129 2129 a, t dbSNP:187055500
2135 2135 a, g dbSNP:772253161
2165 2165 a, g dbSNP:549838567
2226 2226 c, g dbSNP:74517617
2227 2227 a, g dbSNP:80140046
2228 2228 a, t dbSNP:78935901
2229 2229 c, t dbSNP:759551235
2232 2232 c, t dbSNP:781146554
2265 2265 a, g dbSNP:767628237
2273 2273 a, g dbSNP:57390094
2315 2315 a, g dbSNP:184224534

Target ORF information:

RefSeq Version NM_001270524
Organism Homo sapiens (human)
Definition Homo sapiens orthodenticle homeobox 2 (OTX2), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001270524

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGATGTCTT ATCTTAAGCA ACCGCCTTAC GCAGTCAATG GGCTGAGTCT GACCACTTCG 
GGTATGGACT TGCTGCACCC CTCCGTGGGC TACCCGGCCA CCCCCCGGAA ACAGCGCCGG
GAGAGGACGA CGTTCACTCG GGCGCAGCTA GATGTGCTGG AAGCACTGTT TGCCAAGACC
CGGTACCCAG ACATCTTCAT GCGAGAGGAG GTGGCACTGA AAATCAACTT GCCCGAGTCG
AGGGTGCAGG TATGGTTTAA GAATCGAAGA GCTAAGTGCC GCCAACAACA GCAACAACAG
CAGAATGGAG GTCAAAACAA AGTGAGACCT GCCAAAAAGA AGACATCTCC AGCTCGGGAA
GTGAGTTCAG AGAGTGGAAC AAGTGGCCAA TTCACTCCCC CCTCTAGCAC CTCAGTCCCG
ACCATTGCCA GCAGCAGTGC TCCTGTGTCT ATCTGGAGCC CAGCTTCCAT CTCCCCACTG
TCAGATCCCT TGTCCACCTC CTCTTCCTGC ATGCAGAGGT CCTATCCCAT GACCTATACT
CAGGCTTCAG GTTATAGTCA AGGATATGCT GGCTCAACTT CCTACTTTGG GGGCATGGAC
TGTGGATCAT ATTTGACCCC TATGCATCAC CAGCTTCCCG GACCAGGGGC CACACTCAGT
CCCATGGGTA CCAATGCAGT CACCAGCCAT CTCAATCAGT CCCCAGCTTC TCTTTCCACC
CAGGGATATG GAGCTTCAAG CTTGGGTTTT AACTCAACCA CTGATTGCTT GGATTATAAG
GACCAAACTG CCTCCTGGAA GCTTAACTTC AATGCTGACT GCTTGGATTA TAAAGATCAG
ACATCCTCGT GGAAATTCCA GGTTTTGTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu16734D
Sequence Information ORF Nucleotide Sequence (Length: 894bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product homeobox protein OTX2 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB593056.1, BU176852.1 and BC032579.1. Summary: This gene encodes a member of the bicoid subfamily of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and plays a role in brain, craniofacial, and sensory organ development. The encoded protein also influences the proliferation and differentiation of dopaminergic neuronal progenitor cells during mitosis. Mutations in this gene cause syndromic microphthalmia 5 (MCOPS5) and combined pituitary hormone deficiency 6 (CPHD6). This gene is also suspected of having an oncogenic role in medulloblastoma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Pseudogenes of this gene are known to exist on chromosomes two and nine. [provided by RefSeq, Jul 2012]. Transcript Variant: This variant (5) differs in the 5' UTR, compared to variant 1, and encodes the longer isoform (a). Variants 1 and 5 encode the same protein (isoform a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU176441.1, BU176852.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2148093, SAMEA2151119 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGATGTCTT ATCTTAAGCA ACCGCCTTAC GCAGTCAATG GGCTGAGTCT GACCACTTCG 
GGTATGGACT TGCTGCACCC CTCCGTGGGC TACCCGGGGC CCTGGGCTTC TTGTCCCGCA
GCCACCCCCC GGAAACAGCG CCGGGAGAGG ACGACGTTCA CTCGGGCGCA GCTAGATGTG
CTGGAAGCAC TGTTTGCCAA GACCCGGTAC CCAGACATCT TCATGCGAGA GGAGGTGGCA
CTGAAAATCA ACTTGCCCGA GTCGAGGGTG CAGGTATGGT TTAAGAATCG AAGAGCTAAG
TGCCGCCAAC AACAGCAACA ACAGCAGAAT GGAGGTCAAA ACAAAGTGAG ACCTGCCAAA
AAGAAGACAT CTCCAGCTCG GGAAGTGAGT TCAGAGAGTG GAACAAGTGG CCAATTCACT
CCCCCCTCTA GCACCTCAGT CCCGACCATT GCCAGCAGCA GTGCTCCTGT GTCTATCTGG
AGCCCAGCTT CCATCTCCCC ACTGTCAGAT CCCTTGTCCA CCTCCTCTTC CTGCATGCAG
AGGTCCTATC CCATGACCTA TACTCAGGCT TCAGGTTATA GTCAAGGATA TGCTGGCTCA
ACTTCCTACT TTGGGGGCAT GGACTGTGGA TCATATTTGA CCCCTATGCA TCACCAGCTT
CCCGGACCAG GGGCCACACT CAGTCCCATG GGTACCAATG CAGTCACCAG CCATCTCAAT
CAGTCCCCAG CTTCTCTTTC CACCCAGGGA TATGGAGCTT CAAGCTTGGG TTTTAACTCA
ACCACTGATT GCTTGGATTA TAAGGACCAA ACTGCCTCCT GGAAGCTTAA CTTCAATGCT
GACTGCTTGG ATTATAAAGA TCAGACATCC TCGTGGAAAT TCCAGGTTTT GTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001257454.1
CDS208..1101
Misc Feature(1)49..51(+)
Misc Feature(2)346..513(+)
Misc Feature(3)346..513(+)
Misc Feature(4)352..504(+)
Misc Feature(5)688..936(+)
Exon (1)1..304
Gene:OTX2
Gene Synonym:
Exon (2)305..480
Gene:OTX2
Gene Synonym:
Exon (3)481..2129
Gene:OTX2
Gene Synonym:
Translation
Position Chain Variation Link
116 116 c, g dbSNP:566671671
126 126 c, g dbSNP:540831263
157 157 a, t dbSNP:546858272
158 158 a, g dbSNP:747816534
161 161 c, g dbSNP:776104541
162 162 a, g dbSNP:200002729
164 164 c, t dbSNP:373972732
165 165 a, g dbSNP:564618315
167 167 c, t dbSNP:779606022
170 170 c, t dbSNP:758339288
175 175 c, t dbSNP:546311189
179 179 a, c dbSNP:370535121
181 181 c, g dbSNP:757069643
187 187 c, g dbSNP:754146678
191 191 c, t dbSNP:764157150
199 199 a, g dbSNP:756345000
201 201 a, c dbSNP:752785578
211 211 a, g dbSNP:767603643
216 216 c, t dbSNP:575736431
222 222 g, t dbSNP:752010967
232 232 c, t dbSNP:766756721
238 238 g, t dbSNP:763234678
245 245 a, g dbSNP:776356711
246 246 c, t dbSNP:768254721
249 249 g, t dbSNP:760374061
264 264 c, t dbSNP:774907916
265 265 c, t dbSNP:771687556
269 269 a, g dbSNP:745822138
270 270 -, t dbSNP:779418668
271 271 a, g dbSNP:200680654
273 273 c, g dbSNP:778956556
276 276 a, c dbSNP:770855748
283 283 c, g dbSNP:749151724
288 288 -, c dbSNP:786205874
292 292 a, g dbSNP:777618282
296 296 a, g dbSNP:756331034
302 302 c, t dbSNP:752941534
307 307 c, t dbSNP:371205339
313 313 c, g dbSNP:761773984
322 322 a, c dbSNP:751692939
325 325 a, g dbSNP:780106127
332 332 a, c dbSNP:758946986
333 333 c, t dbSNP:750923926
334 334 c, g dbSNP:374042850
337 337 -, c dbSNP:758119168
369 369 c, t dbSNP:757602259
383 383 a, g dbSNP:567955379
394 394 g, t dbSNP:368759331
399 399 a, g dbSNP:141664597
410 410 c, t dbSNP:759256101
411 411 c, t dbSNP:774043425
414 414 a, g dbSNP:765964221
420 420 a, g dbSNP:762821214
433 433 a, c dbSNP:773157352
465 465 c, t dbSNP:769477143
466 466 a, g dbSNP:786205224
471 471 a, g dbSNP:376651816
477 477 g, t dbSNP:747916036
496 496 c, g dbSNP:104894464
497 497 a, g dbSNP:200990681
514 514 a, c dbSNP:78953717
516 516 a, g dbSNP:749788509
523 523 -, c dbSNP:786205884
537 537 c, t dbSNP:778304481
543 543 c, t dbSNP:536495343
548 548 a, g dbSNP:751258203
557 557 c, g dbSNP:779696471
558 558 a, c dbSNP:757971368
559 559 a, c dbSNP:750013761
569 569 a, g dbSNP:764625033
573 573 a, g dbSNP:761862286
578 578 c, t dbSNP:753729345
579 579 c, t dbSNP:763911822
580 580 c, t dbSNP:760396081
586 586 c, t dbSNP:775702810
587 587 a, c, g dbSNP:199799627
593 593 g, t dbSNP:774537529
595 595 a, g dbSNP:748205899
603 603 a, g dbSNP:749843466
608 608 c, g dbSNP:778357805
613 613 a, g dbSNP:770291855
618 618 c, t dbSNP:772021946
619 619 c, t dbSNP:74658806
625 625 a, c, g dbSNP:758106958
628 628 a, c dbSNP:376333965
630 630 a, c, t dbSNP:372008404
631 631 c, g dbSNP:753783256
632 632 a, c, g dbSNP:199761861
633 633 c, t dbSNP:553884027
644 644 c, g dbSNP:786205879
650 650 c, t dbSNP:201721391
651 651 a, c, g dbSNP:147896150
653 653 c, t dbSNP:771047348
654 654 c, t dbSNP:763064579
658 658 a, g, t dbSNP:770486100
659 659 c, t dbSNP:748631596
660 660 c, t dbSNP:777104497
662 662 a, g dbSNP:768857621
666 666 c, t dbSNP:34537598
671 671 c, g, t dbSNP:200171280
684 684 a, c dbSNP:373798705
690 690 c, t dbSNP:538192166
695 695 -, gc dbSNP:786205873
701 701 a, t dbSNP:756087368
711 711 g, t dbSNP:752526255
721 721 a, t dbSNP:370354056
732 732 c, t dbSNP:767374937
747 747 a, g dbSNP:368033398
757 757 c, t dbSNP:751820068
759 759 a, c dbSNP:766762080
768 768 a, c, t dbSNP:104894465
781 781 g, t dbSNP:773463323
787 787 a, g dbSNP:765382721
788 788 a, g dbSNP:762435469
791 791 a, g dbSNP:776964451
793 793 g, t dbSNP:397514463
794 794 g, t dbSNP:142205965
798 798 c, t dbSNP:747279627
804 804 c, g dbSNP:775913832
808 808 a, g, t dbSNP:748910557
809 809 a, c, t dbSNP:755578825
848 848 a, c dbSNP:150982073
850 850 a, c dbSNP:780929725
862 862 c, g dbSNP:754929157
863 863 a, c dbSNP:751376563
868 868 c, t dbSNP:548455339
870 870 c, t dbSNP:142743327
871 871 a, g dbSNP:374968145
880 880 a, g dbSNP:750626422
884 884 c, t dbSNP:765441049
887 887 g, t dbSNP:761733794
889 889 a, g dbSNP:529946021
892 892 -, cccatgggt dbSNP:745480462
900 900 c, t dbSNP:764571884
905 905 a, g dbSNP:370761964
912 912 c, t dbSNP:775876318
920 920 a, g, t dbSNP:144449264
922 922 c, t dbSNP:772782540
931 931 c, t dbSNP:769304167
938 938 c, t dbSNP:747665958
946 946 c, t dbSNP:143131956
950 950 c, t dbSNP:199650198
955 955 c, g dbSNP:768514532
956 956 c, g dbSNP:746941491
964 964 a, g dbSNP:139800030
965 965 c, t dbSNP:758228717
971 971 a, g dbSNP:781096873
989 989 c, t dbSNP:750679604
1004 1004 a, c dbSNP:779220317
1038 1038 c, t dbSNP:78559885
1047 1047 c, t dbSNP:201396096
1051 1051 a, t dbSNP:764205900
1073 1073 c, t dbSNP:761138937
1079 1079 c, t dbSNP:753156713
1080 1080 a, c, g dbSNP:759833842
1088 1088 c, t dbSNP:774680300
1103 1103 a, g dbSNP:370275924
1106 1106 a, c, t dbSNP:776053080
1111 1111 a, g dbSNP:171978
1127 1127 a, c, g dbSNP:780072062
1131 1131 c, t dbSNP:772006682
1135 1135 a, t dbSNP:182392988
1142 1142 a, g dbSNP:3742576
1144 1144 g, t dbSNP:558072316
1147 1147 a, g dbSNP:757464106
1222 1222 a, g dbSNP:575988911
1225 1225 c, t dbSNP:555242082
1226 1226 c, t dbSNP:561466216
1227 1227 a, c, g dbSNP:750146313
1247 1247 c, t dbSNP:543291377
1261 1261 a, g dbSNP:75947588
1264 1264 a, g dbSNP:764880954
1362 1362 c, g dbSNP:553969803
1374 1374 c, t dbSNP:538805998
1417 1417 a, g dbSNP:142727455
1440 1440 c, t dbSNP:556614831
1441 1441 -, c dbSNP:3215889
1446 1446 a, c dbSNP:537889967
1460 1460 g, t dbSNP:567634820
1488 1488 g, t dbSNP:372333803
1525 1525 a, c dbSNP:778705827
1528 1528 a, g dbSNP:761387465
1537 1537 c, g dbSNP:190556214
1592 1592 a, g dbSNP:369779296
1593 1593 -, attg dbSNP:149455658
1610 1610 a, g dbSNP:754230866
1696 1696 a, g dbSNP:764392879
1734 1734 c, t dbSNP:536571793
1768 1768 a, c dbSNP:184421963
1778 1778 c, t dbSNP:56245644
1799 1799 a, t dbSNP:58246365
1823 1823 a, g dbSNP:192422162
1865 1865 a, g dbSNP:775857186
1880 1880 a, g dbSNP:138197536
1923 1923 a, t dbSNP:187055500
1929 1929 a, g dbSNP:772253161
1959 1959 a, g dbSNP:549838567
2020 2020 c, g dbSNP:74517617
2021 2021 a, g dbSNP:80140046
2022 2022 a, t dbSNP:78935901
2023 2023 c, t dbSNP:759551235
2026 2026 c, t dbSNP:781146554
2059 2059 a, g dbSNP:767628237
2067 2067 a, g dbSNP:57390094
2109 2109 a, g dbSNP:184224534

Target ORF information:

RefSeq Version NM_001270525
Organism Homo sapiens (human)
Definition Homo sapiens orthodenticle homeobox 2 (OTX2), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001270525

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGATGTCTT ATCTTAAGCA ACCGCCTTAC GCAGTCAATG GGCTGAGTCT GACCACTTCG 
GGTATGGACT TGCTGCACCC CTCCGTGGGC TACCCGGGGC CCTGGGCTTC TTGTCCCGCA
GCCACCCCCC GGAAACAGCG CCGGGAGAGG ACGACGTTCA CTCGGGCGCA GCTAGATGTG
CTGGAAGCAC TGTTTGCCAA GACCCGGTAC CCAGACATCT TCATGCGAGA GGAGGTGGCA
CTGAAAATCA ACTTGCCCGA GTCGAGGGTG CAGGTATGGT TTAAGAATCG AAGAGCTAAG
TGCCGCCAAC AACAGCAACA ACAGCAGAAT GGAGGTCAAA ACAAAGTGAG ACCTGCCAAA
AAGAAGACAT CTCCAGCTCG GGAAGTGAGT TCAGAGAGTG GAACAAGTGG CCAATTCACT
CCCCCCTCTA GCACCTCAGT CCCGACCATT GCCAGCAGCA GTGCTCCTGT GTCTATCTGG
AGCCCAGCTT CCATCTCCCC ACTGTCAGAT CCCTTGTCCA CCTCCTCTTC CTGCATGCAG
AGGTCCTATC CCATGACCTA TACTCAGGCT TCAGGTTATA GTCAAGGATA TGCTGGCTCA
ACTTCCTACT TTGGGGGCAT GGACTGTGGA TCATATTTGA CCCCTATGCA TCACCAGCTT
CCCGGACCAG GGGCCACACT CAGTCCCATG GGTACCAATG CAGTCACCAG CCATCTCAAT
CAGTCCCCAG CTTCTCTTTC CACCCAGGGA TATGGAGCTT CAAGCTTGGG TTTTAACTCA
ACCACTGATT GCTTGGATTA TAAGGACCAA ACTGCCTCCT GGAAGCTTAA CTTCAATGCT
GACTGCTTGG ATTATAAAGA TCAGACATCC TCGTGGAAAT TCCAGGTTTT GTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.