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OXCT1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol OXCT1
Entrez Gene ID 5019
Full Name 3-oxoacid CoA transferase 1
Synonyms OXCT, SCOT
General protein information
Preferred Names
succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial
Names
succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial
SCOT-s
3-oxoacid CoA-transferase 1
3-oxoacid-CoA transferase 1
succinyl CoA:3-oxoacid CoA transferase
succinyl-CoA:3-ketoacid-CoA transferase
somatic-type succinyl CoA:3-oxoacid CoA-transferase
somatic-type succinyl-CoA:3-oxoacid CoA-transferase
somatic-type succinyl-CoA:3-oxoacid-CoA-transferase
succinyl-CoA:3-ketoacid-coenzyme A transferase 1, mitochondrial
NP_000427.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

5

5p13.1

Summary This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

601424

Disorder Html: Succinyl CoA:3-oxoacid CoA transferase deficiency, 245050 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000436 NP_000427 succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial precursor



Homo sapiens (human) OXCT1 NP_000427.1
Pan troglodytes (chimpanzee) OXCT1 XP_001137948.1
Macaca mulatta (Rhesus monkey) OXCT1 XP_001114587.1
Canis lupus familiaris (dog) OXCT1 XP_536487.2
Bos taurus (cattle) OXCT1 NP_001069538.1
Mus musculus (house mouse) Oxct1 NP_077150.1
Rattus norvegicus (Norway rat) Oxct1 NP_001121052.1
Gallus gallus (chicken) OXCT1 NP_001006578.2
Danio rerio (zebrafish) oxct1a NP_001007292.1
Danio rerio (zebrafish) oxct1b NP_001070618.1
Drosophila melanogaster (fruit fly) CG1140 NP_647683.1
Caenorhabditis elegans C05C10.3 NP_496144.1
Xenopus (Silurana) tropicalis (western clawed frog) oxct1 NP_001007927.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following OXCT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OXCT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu17913
NM_000436 Homo sapiens 3-oxoacid CoA transferase 1 (OXCT1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $189.50
$379.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu17913
Clone ID Related Accession (Same CDS sequence) NM_000436
Accession Version NM_000436.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC034222.5 and BC009001.1. This sequence is a reference standard in the RefSeqGene project. On Aug 16, 2006 this sequence version replaced gi:47458829. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC009001.1, U62961.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCGGCTC TCAAACTCCT CTCCTCCGGG CTTCGGCTCT GCGCCTCTGC CCGCGGATCT 
GGGGCAACCT GGTACAAGGG ATGTGTTTGT TCCTTTTCCA CCAGTGCTCA TCGCCATACC
AAGTTTTATA CAGATCCAGT AGAAGCTGTA AAAGACATCC CTGATGGTGC CACGGTTTTG
GTTGGTGGTT TTGGGCTATG TGGAATTCCA GAGAATCTTA TAGATGCTTT ACTGAAAACT
GGAGTAAAAG GACTAACTGC AGTCAGCAAC AATGCAGGGG TTGACAATTT TGGTTTGGGG
CTTTTGCTTC GGTCCAAGCA GATAAAACGC ATGGTCTCTT CATATGTGGG AGAAAATGCA
GAATTTGAAC GACAGTACTT ATCTGGTGAA TTAGAAGTGG AGCTGACACC ACAGGGCACA
CTTGCAGAGA GGATCCGTGC AGGCGGGGCT GGAGTTCCTG CATTTTACAC CCCAACAGGG
TATGGGACCC TGGTACAAGA AGGAGGATCG CCCATCAAAT ACAACAAAGA TGGCAGTGTT
GCCATTGCCA GTAAGCCAAG AGAGGTGAGG GAGTTCAATG GTCAGCACTT TATTTTGGAG
GAAGCAATTA CAGGGGATTT TGCTTTGGTG AAAGCCTGGA AGGCGGACCG AGCAGGAAAC
GTGATTTTCA GGAAAAGTGC AAGGAATTTC AACTTGCCAA TGTGCAAAGC TGCAGAAACC
ACAGTGGTAG AGGTTGAAGA AATTGTGGAT ATTGGAGCAT TTGCTCCAGA AGACATCCAT
ATTCCTCAGA TTTATGTACA TCGCCTTATA AAGGGAGAAA AATATGAGAA AAGAATTGAG
CGTTTATCAA TCCGGAAAGA GGGAGATGGG GAAGCCAAAT CTGCTAAACC TGGAGATGAC
GTAAGGGAAC GAATCATCAA GAGGGCCGCT CTTGAGTTTG AGGATGGCAT GTATGCTAAT
TTGGGCATAG GAATCCCTCT CCTGGCCAGC AATTTTATCA GCCCAAATAT AACTGTTCAT
CTTCAAAGTG AAAATGGAGT TCTGGGTTTG GGTCCATATC CACGACAACA TGAAGCTGAT
GCAGATCTCA TCAATGCAGG CAAGGAAACA GTTACTATTC TTCCAGGAGC CTCTTTTTTC
TCCAGCGATG AATCATTTGC AATGATTAGA GGTGGACACG TCGATCTGAC AATGCTAGGA
GCGATGCAGG TTTCCAAATA TGGTGACCTG GCTAACTGGA TGATACCTGG GAAGATGGTG
AAAGGAATGG GAGGTGCTAT GGATTTAGTG TCCAGTGCGA AAACCAAAGT GGTGGTCACC
ATGGAGCATT CTGCAAAGGG AAATGCACAT AAAATCATGG AGAAATGTAC ATTACCATTG
ACTGGAAAGC AATGTGTCAA CCGCATTATT ACTGAAAAGG CTGTGTTTGA TGTGGACAAG
AAGAAAGGGT TGACTCTGAT TGAGCTCTGG GAAGGCCTGA CAGTGGATGA CGTACAGAAG
AGTACTGGGT GTGATTTTGC AGTTTCACCA AAACTCATGC CAATGCAGCA GATCGCAAAT
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000427.1
CDS332..1894
Misc Feature(1)164..166(+)
Misc Feature(2)452..1153(+)
Misc Feature(3)473..1882(+)
Misc Feature(4)839..841(+)
Misc Feature(5)1229..1855(+)
Exon (1)1..409
Gene:OXCT1
Gene Synonym:
Exon (2)410..518
Gene:OXCT1
Gene Synonym:
Exon (3)519..609
Gene:OXCT1
Gene Synonym:
Exon (4)610..745
Gene:OXCT1
Gene Synonym:
Exon (5)746..895
Gene:OXCT1
Gene Synonym:
Exon (6)896..1002
Gene:OXCT1
Gene Synonym:
Exon (7)1003..1063
Gene:OXCT1
Gene Synonym:
Exon (8)1064..1171
Gene:OXCT1
Gene Synonym:
Exon (9)1172..1286
Gene:OXCT1
Gene Synonym:
Exon (10)1287..1381
Gene:OXCT1
Gene Synonym:
Exon (11)1382..1430
Gene:OXCT1
Gene Synonym:
Exon (12)1431..1503
Gene:OXCT1
Gene Synonym:
Exon (13)1504..1579
Gene:OXCT1
Gene Synonym:
Exon (14)1580..1669
Gene:OXCT1
Gene Synonym:
Exon (15)1670..1750
Gene:OXCT1
Gene Synonym:
Exon (16)1751..1852
Gene:OXCT1
Gene Synonym:
Exon (17)1853..3558
Gene:OXCT1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000436
Organism Homo sapiens (human)
Definition Homo sapiens 3-oxoacid CoA transferase 1 (OXCT1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000436

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCGGCTC TCAAACTCCT CTCCTCCGGG CTTCGGCTCT GCGCCTCTGC CCGCGGATCT 
GGGGCAACCT GGTACAAGGG ATGTGTTTGT TCCTTTTCCA CCAGTGCTCA TCGCCATACC
AAGTTTTATA CAGATCCAGT AGAAGCTGTA AAAGACATCC CTGATGGTGC CACGGTTTTG
GTTGGTGGTT TTGGGCTATG TGGAATTCCA GAGAATCTTA TAGATGCTTT ACTGAAAACT
GGAGTAAAAG GACTAACTGC AGTCAGCAAC AATGCAGGGG TTGACAATTT TGGTTTGGGG
CTTTTGCTTC GGTCCAAGCA GATAAAACGC ATGGTCTCTT CATATGTGGG AGAAAATGCA
GAATTTGAAC GACAGTACTT ATCTGGTGAA TTAGAAGTGG AGCTGACACC ACAGGGCACA
CTTGCAGAGA GGATCCGTGC AGGCGGGGCT GGAGTTCCTG CATTTTACAC CCCAACAGGG
TATGGGACCC TGGTACAAGA AGGAGGATCG CCCATCAAAT ACAACAAAGA TGGCAGTGTT
GCCATTGCCA GTAAGCCAAG AGAGGTGAGG GAGTTCAATG GTCAGCACTT TATTTTGGAG
GAAGCAATTA CAGGGGATTT TGCTTTGGTG AAAGCCTGGA AGGCGGACCG AGCAGGAAAC
GTGATTTTCA GGAAAAGTGC AAGGAATTTC AACTTGCCAA TGTGCAAAGC TGCAGAAACC
ACAGTGGTAG AGGTTGAAGA AATTGTGGAT ATTGGAGCAT TTGCTCCAGA AGACATCCAT
ATTCCTCAGA TTTATGTACA TCGCCTTATA AAGGGAGAAA AATATGAGAA AAGAATTGAG
CGTTTATCAA TCCGGAAAGA GGGAGATGGG GAAGCCAAAT CTGCTAAACC TGGAGATGAC
GTAAGGGAAC GAATCATCAA GAGGGCCGCT CTTGAGTTTG AGGATGGCAT GTATGCTAAT
TTGGGCATAG GAATCCCTCT CCTGGCCAGC AATTTTATCA GCCCAAATAT AACTGTTCAT
CTTCAAAGTG AAAATGGAGT TCTGGGTTTG GGTCCATATC CACGACAACA TGAAGCTGAT
GCAGATCTCA TCAATGCAGG CAAGGAAACA GTTACTATTC TTCCAGGAGC CTCTTTTTTC
TCCAGCGATG AATCATTTGC AATGATTAGA GGTGGACACG TCGATCTGAC AATGCTAGGA
GCGATGCAGG TTTCCAAATA TGGTGACCTG GCTAACTGGA TGATACCTGG GAAGATGGTG
AAAGGAATGG GAGGTGCTAT GGATTTAGTG TCCAGTGCGA AAACCAAAGT GGTGGTCACC
ATGGAGCATT CTGCAAAGGG AAATGCACAT AAAATCATGG AGAAATGTAC ATTACCATTG
ACTGGAAAGC AATGTGTCAA CCGCATTATT ACTGAAAAGG CTGTGTTTGA TGTGGACAAG
AAGAAAGGGT TGACTCTGAT TGAGCTCTGG GAAGGCCTGA CAGTGGATGA CGTACAGAAG
AGTACTGGGT GTGATTTTGC AGTTTCACCA AAACTCATGC CAATGCAGCA GATCGCAAAT
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Clinical and molecular characterization of five patients with succinyl-CoA:3-ketoacid CoA transferase (SCOT) deficiency
Biochim. Biophys. Acta 1812 (5), 619-624 (2011)
Fukao T, Sass JO, Kursula P, Thimm E, Wendel U, Ficicioglu C, Monastiri K, Guffon N, Baric I, Zabot MT and Kondo N.


book

Differential utilization of ketone bodies by neurons and glioma cell lines: a rationale for ketogenic diet as experimental glioma therapy
BMC Cancer 11, 315 (2011)
Maurer GD, Brucker DP, Bahr O, Harter PN, Hattingen E, Walenta S, Mueller-Klieser W, Steinbach JP and Rieger J.


book

A neonatal-onset succinyl-CoA:3-ketoacid CoA transferase (SCOT)-deficient patient with T435N and c.658-666dupAACGTGATT p.N220_I222dup mutations in the OXCT1 gene
J. Inherit. Metab. Dis. 33 (SUPPL 3), S307-S313 (2010)
Fukao T, Ishii T, Amano N, Kursula P, Takayanagi M, Murase K, Sakaguchi N, Kondo N and Hasegawa T.


book

Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia
J Psychiatr Res 44 (14), 989-991 (2010)
Martins-de-Souza D, Schmitt A, Roder R, Lebar M, Schneider-Axmann T, Falkai P and Turck CW.


book

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression
PLoS ONE 5 (9), E12862 (2010)
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL and O'Brien SJ.


book

Succinyl-CoA:3-ketoacid CoA transferase (SCOT) deficiency: two pathogenic mutations, V133E and C456F, in Japanese siblings
Hum. Mutat. 12 (2), 83-88 (1998)
Song XQ, Fukao T, Watanabe H, Shintaku H, Hirayama K, Kassovska-Bratinova S, Kondo N and Mitchell GA.


book

Enzymes of ketone body utilization in human tissues: protein and messenger RNA levels of succinyl-coenzyme A (CoA):3-ketoacid CoA transferase and mitochondrial and cytosolic acetoacetyl-CoA thiolases
Pediatr. Res. 42 (4), 498-502 (1997)
Fukao T, Song XQ, Mitchell GA, Yamaguchi S, Sukegawa K, Orii T and Kondo N.


book

Succinyl CoA: 3-oxoacid CoA transferase (SCOT): human cDNA cloning, human chromosomal mapping to 5p13, and mutation detection in a SCOT-deficient patient
Am. J. Hum. Genet. 59 (3), 519-528 (1996)
Kassovska-Bratinova S, Fukao T, Song XQ, Duncan AM, Chen HS, Robert MF, Perez-Cerda C, Ugarte M, Chartrand C, Vobecky S, Kondo N and Mitchell GA.


book

A new case of succinyl-CoA: acetoacetate transferase deficiency
J. Inherit. Metab. Dis. 15 (3), 371-373 (1992)
Perez-Cerda C, Merinero B, Sanz P, Jimenez A, Hernandez C, Garcia MJ and Ugarte M.


book

Acetoacetate utilization by human placental mitochondria
Placenta 5 (3), 271-276 (1984)
Zolnierowicz,S., Scislowski,P.W., Swierczynski,J. and Zelewski,L.


 
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