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PAH cDNA ORF clone, Homo sapiens (human)

Gene Symbol PAH
Entrez Gene ID 5053
Full Name phenylalanine hydroxylase
Synonyms PH, PKU, PKU1
General protein information
Preferred Names
phenylalanine-4-hydroxylase
Names
phenylalanine-4-hydroxylase
phe-4-monooxygenase
phenylalanine 4-monooxygenase
NP_000268.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q22-q24.2

Summary PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

612349

Disorder Html: Phenylketonuria, 261600 (3); [Hyperphenylalaninemia, non-PKU mild],

mRNA and Protein(s)

mRNA Protein Name
XM_011538422 XP_011536724 phenylalanine-4-hydroxylase isoform X1
NM_000277 NP_000268 phenylalanine-4-hydroxylase



Homo sapiens (human) PAH NP_000268.1
Pan troglodytes (chimpanzee) PAH XP_001156919.1
Macaca mulatta (Rhesus monkey) PAH XP_001094859.1
Canis lupus familiaris (dog) PAH XP_532671.3
Bos taurus (cattle) PAH NP_001039523.1
Mus musculus (house mouse) Pah NP_032803.2
Rattus norvegicus (Norway rat) Pah NP_036751.2
Gallus gallus (chicken) PAH NP_001001298.1
Danio rerio (zebrafish) pah NP_956845.1
Drosophila melanogaster (fruit fly) Hn NP_001261542.1
Caenorhabditis elegans pah-1 NP_001254185.1
Xenopus (Silurana) tropicalis (western clawed frog) pah NP_001027484.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PAH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PAH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu59464 XM_011538422 PREDICTED: Homo sapiens phenylalanine hydroxylase (PAH), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu25787
NM_000277 Homo sapiens phenylalanine hydroxylase (PAH), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu59464
Clone ID Related Accession (Same CDS sequence) XM_011538422
Accession Version XM_011538422.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1302bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product phenylalanine-4-hydroxylase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTCCACTG CGGTCCTGGA AAACCCAGGC TTGGGCAGGA AACTCTCTGA CTTTGGACAG 
GAAACAAGCT ATATTGAAGA CAACTGCAAT CAAAATGGTG CCATATCACT GATCTTCTCA
CTCAAAGAAG AAGTTGGTGC ATTGGCCAAA GTATTGCGCT TATTTGAGGA GAATGATGTA
AACCTGACCC ACATTGAATC TAGACCTTCT CGTTTAAAGA AAGATGAGTA TGAATTTTTC
ACCCATTTGG ATAAACGTAG CCTGCCTGCT CTGACAAACA TCATCAAGAT CTTGAGGCAT
GACATTGGTG CCACTGTCCA TGAGCTTTCA CGAGATAAGA AGAAAGACAC AGTGCCCTGG
TTCCCAAGAA CCATTCAAGA GCTGGACAGA TTTGCCAATC AGATTCTCAG CTATGGAGCG
GAACTGGATG CTGACCACCC TGGTTTTAAA GATCCTGTGT ACCGTGCAAG ACGGAAGCAG
TTTGCTGACA TTGCCTACAA CTACCGCCAT GGGCAGCCCA TCCCTCGAGT GGAATACATG
GAGGAAGAAA AGAAAACATG GGGCACAGTG TTCAAGACTC TGAAGTCCTT GTATAAAACC
CATGCTTGCT ATGAGTACAA TCACATTTTT CCACTTCTTG AAAAGTACTG TGGCTTCCAT
GAAGATAACA TTCCCCAGCT GGAAGACGTT TCTCAGTTCC TGCAGACTTG CACTGGTTTC
CGCCTCCGAC CTGTGGCTGG CCTGCTTTCC TCTCGGGATT TCTTGGGTGG CCTGGCCTTC
CGAGTCTTCC ACTGCACACA GTACATCAGA CATGGATCCA AGCCCATGTA TACCCCCGAA
CCTGACATCT GCCATGAGCT GTTGGGACAT GTGCCCTTGT TTTCAGATCG CAGCTTTGCC
CAGTTTTCCC AGATTTACTG GTTTACTGTG GAGTTTGGGC TCTGCAAACA AGGAGACTCC
ATAAAGGCAT ATGGTGCTGG GCTCCTGTCA TCCTTTGGTG AATTACAGTA CTGCTTATCA
GAGAAGCCAA AGCTTCTCCC CCTGGAGCTG GAGAAGACAG CCATCCAAAA TTACACTGTC
ACGGAGTTCC AGCCCCTCTA TTACGTGGCA GAGAGTTTTA ATGATGCCAA GGAGAAAGTA
AGGAACTTTG CTGCCACAAT ACCTCGGCCC TTCTCAGTTC GCTACGACCC ATACACCCAA
AGGATTGAGG TCTTGGACAA TACCCAGCAG CTTAAGATTT TGGCTGATTC CATTAACAGT
GAAATTGGAA TCCTTTGCAG TGCCCTCCAG AAAATAAAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011536724.1
CDS116..1417
Misc Feature(1)173..1414(+)
Misc Feature(2)176..445(+)
Misc Feature(3)176..196(+)
Misc Feature(4)251..322(+)
Misc Feature(5)470..1330(+)
Misc Feature(6)527..1108(+)
Misc Feature(7)854..1033(+)
Misc Feature(8)968..1048(+)
Translation

Target ORF information:

RefSeq Version XM_011538422
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phenylalanine hydroxylase (PAH), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011538422

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTCCACTG CGGTCCTGGA AAACCCAGGC TTGGGCAGGA AACTCTCTGA CTTTGGACAG 
GAAACAAGCT ATATTGAAGA CAACTGCAAT CAAAATGGTG CCATATCACT GATCTTCTCA
CTCAAAGAAG AAGTTGGTGC ATTGGCCAAA GTATTGCGCT TATTTGAGGA GAATGATGTA
AACCTGACCC ACATTGAATC TAGACCTTCT CGTTTAAAGA AAGATGAGTA TGAATTTTTC
ACCCATTTGG ATAAACGTAG CCTGCCTGCT CTGACAAACA TCATCAAGAT CTTGAGGCAT
GACATTGGTG CCACTGTCCA TGAGCTTTCA CGAGATAAGA AGAAAGACAC AGTGCCCTGG
TTCCCAAGAA CCATTCAAGA GCTGGACAGA TTTGCCAATC AGATTCTCAG CTATGGAGCG
GAACTGGATG CTGACCACCC TGGTTTTAAA GATCCTGTGT ACCGTGCAAG ACGGAAGCAG
TTTGCTGACA TTGCCTACAA CTACCGCCAT GGGCAGCCCA TCCCTCGAGT GGAATACATG
GAGGAAGAAA AGAAAACATG GGGCACAGTG TTCAAGACTC TGAAGTCCTT GTATAAAACC
CATGCTTGCT ATGAGTACAA TCACATTTTT CCACTTCTTG AAAAGTACTG TGGCTTCCAT
GAAGATAACA TTCCCCAGCT GGAAGACGTT TCTCAGTTCC TGCAGACTTG CACTGGTTTC
CGCCTCCGAC CTGTGGCTGG CCTGCTTTCC TCTCGGGATT TCTTGGGTGG CCTGGCCTTC
CGAGTCTTCC ACTGCACACA GTACATCAGA CATGGATCCA AGCCCATGTA TACCCCCGAA
CCTGACATCT GCCATGAGCT GTTGGGACAT GTGCCCTTGT TTTCAGATCG CAGCTTTGCC
CAGTTTTCCC AGATTTACTG GTTTACTGTG GAGTTTGGGC TCTGCAAACA AGGAGACTCC
ATAAAGGCAT ATGGTGCTGG GCTCCTGTCA TCCTTTGGTG AATTACAGTA CTGCTTATCA
GAGAAGCCAA AGCTTCTCCC CCTGGAGCTG GAGAAGACAG CCATCCAAAA TTACACTGTC
ACGGAGTTCC AGCCCCTCTA TTACGTGGCA GAGAGTTTTA ATGATGCCAA GGAGAAAGTA
AGGAACTTTG CTGCCACAAT ACCTCGGCCC TTCTCAGTTC GCTACGACCC ATACACCCAA
AGGATTGAGG TCTTGGACAA TACCCAGCAG CTTAAGATTT TGGCTGATTC CATTAACAGT
GAAATTGGAA TCCTTTGCAG TGCCCTCCAG AAAATAAAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25787
Clone ID Related Accession (Same CDS sequence) NM_000277
Accession Version NM_000277.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1359bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product phenylalanine-4-hydroxylase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U49897.1. This sequence is a reference standard in the RefSeqGene project. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U49897.1, K03020.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTCCACTG CGGTCCTGGA AAACCCAGGC TTGGGCAGGA AACTCTCTGA CTTTGGACAG 
GAAACAAGCT ATATTGAAGA CAACTGCAAT CAAAATGGTG CCATATCACT GATCTTCTCA
CTCAAAGAAG AAGTTGGTGC ATTGGCCAAA GTATTGCGCT TATTTGAGGA GAATGATGTA
AACCTGACCC ACATTGAATC TAGACCTTCT CGTTTAAAGA AAGATGAGTA TGAATTTTTC
ACCCATTTGG ATAAACGTAG CCTGCCTGCT CTGACAAACA TCATCAAGAT CTTGAGGCAT
GACATTGGTG CCACTGTCCA TGAGCTTTCA CGAGATAAGA AGAAAGACAC AGTGCCCTGG
TTCCCAAGAA CCATTCAAGA GCTGGACAGA TTTGCCAATC AGATTCTCAG CTATGGAGCG
GAACTGGATG CTGACCACCC TGGTTTTAAA GATCCTGTGT ACCGTGCAAG ACGGAAGCAG
TTTGCTGACA TTGCCTACAA CTACCGCCAT GGGCAGCCCA TCCCTCGAGT GGAATACATG
GAGGAAGAAA AGAAAACATG GGGCACAGTG TTCAAGACTC TGAAGTCCTT GTATAAAACC
CATGCTTGCT ATGAGTACAA TCACATTTTT CCACTTCTTG AAAAGTACTG TGGCTTCCAT
GAAGATAACA TTCCCCAGCT GGAAGACGTT TCTCAATTCC TGCAGACTTG CACTGGTTTC
CGCCTCCGAC CTGTGGCTGG CCTGCTTTCC TCTCGGGATT TCTTGGGTGG CCTGGCCTTC
CGAGTCTTCC ACTGCACACA GTACATCAGA CATGGATCCA AGCCCATGTA TACCCCCGAA
CCTGACATCT GCCATGAGCT GTTGGGACAT GTGCCCTTGT TTTCAGATCG CAGCTTTGCC
CAGTTTTCCC AGGAAATTGG CCTTGCCTCT CTGGGTGCAC CTGATGAATA CATTGAAAAG
CTCGCCACAA TTTACTGGTT TACTGTGGAG TTTGGGCTCT GCAAACAAGG AGACTCCATA
AAGGCATATG GTGCTGGGCT CCTGTCATCC TTTGGTGAAT TACAGTACTG CTTATCAGAG
AAGCCAAAGC TTCTCCCCCT GGAGCTGGAG AAGACAGCCA TCCAAAATTA CACTGTCACG
GAGTTCCAGC CCCTGTATTA CGTGGCAGAG AGTTTTAATG ATGCCAAGGA GAAAGTAAGG
AACTTTGCTG CCACAATACC TCGGCCCTTC TCAGTTCGCT ACGACCCATA CACCCAAAGG
ATTGAGGTCT TGGACAATAC CCAGCAGCTT AAGATTTTGG CTGATTCCAT TAACAGTGAA
ATTGGAATCC TTTGCAGTGC CCTCCAGAAA ATAAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000268.1
CDS473..1831
Misc Feature(1)386..388(+)
Misc Feature(2)518..520(+)
Misc Feature(3)530..1828(+)
Misc Feature(4)533..802(+)
Misc Feature(5)533..553(+)
Misc Feature(6)608..679(+)
Misc Feature(7)827..1744(+)
Misc Feature(8)884..1522(+)
Misc Feature(9)1211..1447(+)
Misc Feature(10)1325..1462(+)
Exon (1)1..532
Gene:PAH
Gene Synonym:
Exon (2)533..640
Gene:PAH
Gene Synonym:
Exon (3)641..824
Gene:PAH
Gene Synonym:
Exon (4)825..913
Gene:PAH
Gene Synonym:
Exon (5)914..981
Gene:PAH
Gene Synonym:
Exon (6)982..1178
Gene:PAH
Gene Synonym:
Exon (7)1179..1314
Gene:PAH
Gene Synonym:
Exon (8)1315..1384
Gene:PAH
Gene Synonym:
Exon (9)1385..1441
Gene:PAH
Gene Synonym:
Exon (10)1442..1537
Gene:PAH
Gene Synonym:
Exon (11)1538..1671
Gene:PAH
Gene Synonym:
Exon (12)1672..1787
Gene:PAH
Gene Synonym:
Exon (13)1788..2680
Gene:PAH
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000277
Organism Homo sapiens (human)
Definition Homo sapiens phenylalanine hydroxylase (PAH), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000277

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTCCACTG CGGTCCTGGA AAACCCAGGC TTGGGCAGGA AACTCTCTGA CTTTGGACAG 
GAAACAAGCT ATATTGAAGA CAACTGCAAT CAAAATGGTG CCATATCACT GATCTTCTCA
CTCAAAGAAG AAGTTGGTGC ATTGGCCAAA GTATTGCGCT TATTTGAGGA GAATGATGTA
AACCTGACCC ACATTGAATC TAGACCTTCT CGTTTAAAGA AAGATGAGTA TGAATTTTTC
ACCCATTTGG ATAAACGTAG CCTGCCTGCT CTGACAAACA TCATCAAGAT CTTGAGGCAT
GACATTGGTG CCACTGTCCA TGAGCTTTCA CGAGATAAGA AGAAAGACAC AGTGCCCTGG
TTCCCAAGAA CCATTCAAGA GCTGGACAGA TTTGCCAATC AGATTCTCAG CTATGGAGCG
GAACTGGATG CTGACCACCC TGGTTTTAAA GATCCTGTGT ACCGTGCAAG ACGGAAGCAG
TTTGCTGACA TTGCCTACAA CTACCGCCAT GGGCAGCCCA TCCCTCGAGT GGAATACATG
GAGGAAGAAA AGAAAACATG GGGCACAGTG TTCAAGACTC TGAAGTCCTT GTATAAAACC
CATGCTTGCT ATGAGTACAA TCACATTTTT CCACTTCTTG AAAAGTACTG TGGCTTCCAT
GAAGATAACA TTCCCCAGCT GGAAGACGTT TCTCAATTCC TGCAGACTTG CACTGGTTTC
CGCCTCCGAC CTGTGGCTGG CCTGCTTTCC TCTCGGGATT TCTTGGGTGG CCTGGCCTTC
CGAGTCTTCC ACTGCACACA GTACATCAGA CATGGATCCA AGCCCATGTA TACCCCCGAA
CCTGACATCT GCCATGAGCT GTTGGGACAT GTGCCCTTGT TTTCAGATCG CAGCTTTGCC
CAGTTTTCCC AGGAAATTGG CCTTGCCTCT CTGGGTGCAC CTGATGAATA CATTGAAAAG
CTCGCCACAA TTTACTGGTT TACTGTGGAG TTTGGGCTCT GCAAACAAGG AGACTCCATA
AAGGCATATG GTGCTGGGCT CCTGTCATCC TTTGGTGAAT TACAGTACTG CTTATCAGAG
AAGCCAAAGC TTCTCCCCCT GGAGCTGGAG AAGACAGCCA TCCAAAATTA CACTGTCACG
GAGTTCCAGC CCCTGTATTA CGTGGCAGAG AGTTTTAATG ATGCCAAGGA GAAAGTAAGG
AACTTTGCTG CCACAATACC TCGGCCCTTC TCAGTTCGCT ACGACCCATA CACCCAAAGG
ATTGAGGTCT TGGACAATAC CCAGCAGCTT AAGATTTTGG CTGATTCCAT TAACAGTGAA
ATTGGAATCC TTTGCAGTGC CCTCCAGAAA ATAAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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