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SERPINE1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol SERPINE1
Entrez Gene ID 5054
Full Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Synonyms PAI, PAI-1, PAI1, PLANH1
General protein information
Preferred Names
plasminogen activator inhibitor 1
Names
plasminogen activator inhibitor 1
serpin E1
endothelial plasminogen activator inhibitor
serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7q22.1

Summary This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]. lac of sum
Disorder MIM:

173360

Disorder Html: Plasminogen activator inhibitor, type I, 613329 (3); {Transcription

mRNA and Protein(s)

mRNA Protein Name
NM_000602 NP_000593 plasminogen activator inhibitor 1 precursor



Homo sapiens (human) SERPINE1 NP_000593.1
Pan troglodytes (chimpanzee) SERPINE1 XP_527841.3
Macaca mulatta (Rhesus monkey) SERPINE1 XP_001107647.1
Canis lupus familiaris (dog) SERPINE1 NP_001184024.1
Bos taurus (cattle) SERPINE1 NP_776562.1
Mus musculus (house mouse) Serpine1 NP_032897.2
Rattus norvegicus (Norway rat) Serpine1 NP_036752.1
Danio rerio (zebrafish) serpine1 NP_001108031.1
Xenopus (Silurana) tropicalis (western clawed frog) serpine1 XP_002941247.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SERPINE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SERPINE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu27681
NM_000602 Homo sapiens serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 (SERPINE1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27681
Clone ID Related Accession (Same CDS sequence) NM_000602
Accession Version NM_000602.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1209bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product plasminogen activator inhibitor 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN309001.1, BC010860.1, M16006.1 and AI752990.1. This sequence is a reference standard in the RefSeqGene project. On Apr 5, 2012 this sequence version replaced gi:259155304. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M16006.1, BC010860.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCAGATGT CTCCAGCCCT CACCTGCCTA GTCCTGGGCC TGGCCCTTGT CTTTGGTGAA 
GGGTCTGCTG TGCACCATCC CCCATCCTAC GTGGCCCACC TGGCCTCAGA CTTCGGGGTG
AGGGTGTTTC AGCAGGTGGC GCAGGCCTCC AAGGACCGCA ACGTGGTTTT CTCACCCTAT
GGGGTGGCCT CGGTGTTGGC CATGCTCCAG CTGACAACAG GAGGAGAAAC CCAGCAGCAG
ATTCAAGCAG CTATGGGATT CAAGATTGAT GACAAGGGCA TGGCCCCCGC CCTCCGGCAT
CTGTACAAGG AGCTCATGGG GCCATGGAAC AAGGATGAGA TCAGCACCAC AGACGCGATC
TTCGTCCAGC GGGATCTGAA GCTGGTCCAG GGCTTCATGC CCCACTTCTT CAGGCTGTTC
CGGAGCACGG TCAAGCAAGT GGACTTTTCA GAGGTGGAGA GAGCCAGATT CATCATCAAT
GACTGGGTGA AGACACACAC AAAAGGTATG ATCAGCAACT TGCTTGGGAA AGGAGCCGTG
GACCAGCTGA CACGGCTGGT GCTGGTGAAT GCCCTCTACT TCAACGGCCA GTGGAAGACT
CCCTTCCCCG ACTCCAGCAC CCACCGCCGC CTCTTCCACA AATCAGACGG CAGCACTGTC
TCTGTGCCCA TGATGGCTCA GACCAACAAG TTCAACTATA CTGAGTTCAC CACGCCCGAT
GGCCATTACT ACGACATCCT GGAACTGCCC TACCACGGGG ACACCCTCAG CATGTTCATT
GCTGCCCCTT ATGAAAAAGA GGTGCCTCTC TCTGCCCTCA CCAACATTCT GAGTGCCCAG
CTCATCAGCC ACTGGAAAGG CAACATGACC AGGCTGCCCC GCCTCCTGGT TCTGCCCAAG
TTCTCCCTGG AGACTGAAGT CGACCTCAGG AAGCCCCTAG AGAACCTGGG AATGACCGAC
ATGTTCAGAC AGTTTCAGGC TGACTTCACG AGTCTTTCAG ACCAAGAGCC TCTCCACGTC
GCGCAGGCGC TGCAGAAAGT GAAGATCGAG GTGAACGAGA GTGGCACGGT GGCCTCCTCA
TCCACAGCTG TCATAGTCTC AGCCCGCATG GCCCCCGAGG AGATCATCAT GGACAGACCC
TTCCTCTTTG TGGTCCGGCA CAACCCCACA GGAACAGTCC TTTTCATGGG CCAAGTGATG
GAACCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000593.1
CDS158..1366
Misc Feature(1)230..1363(+)
Misc Feature(2)851..853(+)
Misc Feature(3)1019..1021(+)
Misc Feature(4)1220..1297(+)
Misc Feature(5)1235..1237(+)
Misc Feature(6)1247..1249(+)
Misc Feature(7)1262..1267(+)
Misc Feature(8)1292..1294(+)
Exon (1)1..156
Gene:SERPINE1
Gene Synonym:
Exon (2)157..428
Gene:SERPINE1
Gene Synonym:
Exon (3)429..662
Gene:SERPINE1
Gene Synonym:
Exon (4)663..857
Gene:SERPINE1
Gene Synonym:
Exon (5)858..1056
Gene:SERPINE1
Gene Synonym:
Exon (6)1057..1157
Gene:SERPINE1
Gene Synonym:
Exon (7)1158..1244
Gene:SERPINE1
Gene Synonym:
Exon (8)1245..1328
Gene:SERPINE1
Gene Synonym:
Exon (9)1329..3190
Gene:SERPINE1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000602
Organism Homo sapiens (human)
Definition Homo sapiens serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 (SERPINE1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000602

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCAGATGT CTCCAGCCCT CACCTGCCTA GTCCTGGGCC TGGCCCTTGT CTTTGGTGAA 
GGGTCTGCTG TGCACCATCC CCCATCCTAC GTGGCCCACC TGGCCTCAGA CTTCGGGGTG
AGGGTGTTTC AGCAGGTGGC GCAGGCCTCC AAGGACCGCA ACGTGGTTTT CTCACCCTAT
GGGGTGGCCT CGGTGTTGGC CATGCTCCAG CTGACAACAG GAGGAGAAAC CCAGCAGCAG
ATTCAAGCAG CTATGGGATT CAAGATTGAT GACAAGGGCA TGGCCCCCGC CCTCCGGCAT
CTGTACAAGG AGCTCATGGG GCCATGGAAC AAGGATGAGA TCAGCACCAC AGACGCGATC
TTCGTCCAGC GGGATCTGAA GCTGGTCCAG GGCTTCATGC CCCACTTCTT CAGGCTGTTC
CGGAGCACGG TCAAGCAAGT GGACTTTTCA GAGGTGGAGA GAGCCAGATT CATCATCAAT
GACTGGGTGA AGACACACAC AAAAGGTATG ATCAGCAACT TGCTTGGGAA AGGAGCCGTG
GACCAGCTGA CACGGCTGGT GCTGGTGAAT GCCCTCTACT TCAACGGCCA GTGGAAGACT
CCCTTCCCCG ACTCCAGCAC CCACCGCCGC CTCTTCCACA AATCAGACGG CAGCACTGTC
TCTGTGCCCA TGATGGCTCA GACCAACAAG TTCAACTATA CTGAGTTCAC CACGCCCGAT
GGCCATTACT ACGACATCCT GGAACTGCCC TACCACGGGG ACACCCTCAG CATGTTCATT
GCTGCCCCTT ATGAAAAAGA GGTGCCTCTC TCTGCCCTCA CCAACATTCT GAGTGCCCAG
CTCATCAGCC ACTGGAAAGG CAACATGACC AGGCTGCCCC GCCTCCTGGT TCTGCCCAAG
TTCTCCCTGG AGACTGAAGT CGACCTCAGG AAGCCCCTAG AGAACCTGGG AATGACCGAC
ATGTTCAGAC AGTTTCAGGC TGACTTCACG AGTCTTTCAG ACCAAGAGCC TCTCCACGTC
GCGCAGGCGC TGCAGAAAGT GAAGATCGAG GTGAACGAGA GTGGCACGGT GGCCTCCTCA
TCCACAGCTG TCATAGTCTC AGCCCGCATG GCCCCCGAGG AGATCATCAT GGACAGACCC
TTCCTCTTTG TGGTCCGGCA CAACCCCACA GGAACAGTCC TTTTCATGGG CCAAGTGATG
GAACCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Plasma plasminogen activator inhibitor-1 (PAI-1) levels in breast cancer - relationship with clinical outcome
Anticancer Res. 34 (3), 1153-1161 (2014)
Ferroni P, Roselli M, Portarena I, Formica V, Riondino S, LA Farina F, Costarelli L, Melino A, Massimiani G, Cavaliere F, Palmirotta R and Guadagni F.


book

Association of five common polymorphisms in the plasminogen activator inhibitor-1 gene with primary ovarian insufficiency
Fertil. Steril. 101 (3), 825-832 (2014)
Jeon YJ, Kim YR, Lee BE, Cha SH, Moon MJ, Oh D, Lee WS and Kim NK.


book

[Polymorphism of the plasminogen activator inhibitor type 1 gene, plasminogen level and thrombosis in patients with antiphospholipid syndrome]
Biomed Khim 60 (1), 72-93 (2014)
Aisina,R.B., Mukhametova,L.I., Ostriakova,E.V., Seredavkina,N.V., Patrushev,L.I., Patrusheva,N.L., Reshetniak,T.M., Gulin,D.A., Gershkovich,K.B., Nasonov,E.L. and Varfolomeev,S.D.


book

Prothrombotic factors in histologically proven nonalcoholic fatty liver disease and nonalcoholic steatohepatitis
Hepatology 59 (1), 121-129 (2014)
Verrijken A, Francque S, Mertens I, Prawitt J, Caron S, Hubens G, Van Marck E, Staels B, Michielsen P and Van Gaal L.


book

Mechanistic characterization and crystal structure of a small molecule inactivator bound to plasminogen activator inhibitor-1
Proc. Natl. Acad. Sci. U.S.A. 110 (51), E4941-E4949 (2013)
Li SH, Reinke AA, Sanders KL, Emal CD, Whisstock JC, Stuckey JA and Lawrence DA.


book

Brief report: complete deficiency of plasminogen-activator inhibitor type 1 due to a frame-shift mutation
N. Engl. J. Med. 327 (24), 1729-1733 (1992)
Fay WP, Shapiro AD, Shih JL, Schleef RR and Ginsburg D.


book

Concentration of plasminogen activators and inhibitor in the human endometrium at different phases of the menstrual cycle
J. Reprod. Fertil. 96 (2), 407-413 (1992)
Koh SC, Wong PC, Yuen R, Chua SE, Ng BL and Ratnam SS.


book

Plasminogen activator inhibitor-1 deposition in the extracellular matrix of cultured human mesangial cells
Am. J. Pathol. 141 (1), 117-128 (1992)
Hagege J, Peraldi MN, Rondeau E, Adida C, Delarue F, Medcalf R, Schleuning WD and Sraer JD.


book

Structural basis of latency in plasminogen activator inhibitor-1
Nature 355 (6357), 270-273 (1992)
Mottonen J, Strand A, Symersky J, Sweet RM, Danley DE, Geoghegan KF, Gerard RD and Goldsmith EJ.


book

Alteration of serpin specificity by a protein cofactor. Vitronectin endows plasminogen activator inhibitor 1 with thrombin inhibitory properties
J. Biol. Chem. 265 (22), 13029-13035 (1990)
Ehrlich HJ, Gebbink RK, Keijer J, Linders M, Preissner KT and Pannekoek H.


 
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