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PARK2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol PARK2
Entrez Gene ID 5071
Full Name parkin RBR E3 ubiquitin protein ligase
Synonyms AR-JP, LPRS2, PDJ, PRKN
General protein information
Preferred Names
E3 ubiquitin-protein ligase parkin
Names
E3 ubiquitin-protein ligase parkin
parkinson juvenile disease protein 2
Parkinson disease (autosomal recessive, juvenile) 2, parkin
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6q25.2-q27

Summary The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

602544

Disorder Html: Parkinson disease, juvenile, type 2, 600116 (3); Adenocarcinoma of

mRNA and Protein(s)

mRNA Protein Name
XM_011535863 XP_011534165 E3 ubiquitin-protein ligase parkin isoform X1
XM_011535864 XP_011534166 E3 ubiquitin-protein ligase parkin isoform X2
XM_011535865 XP_011534167 E3 ubiquitin-protein ligase parkin isoform X3
XM_011535866 XP_011534168 E3 ubiquitin-protein ligase parkin isoform X4
XM_011535867 XP_011534169 E3 ubiquitin-protein ligase parkin isoform X5
NM_004562 NP_004553 E3 ubiquitin-protein ligase parkin isoform 1
NM_013987 NP_054642 E3 ubiquitin-protein ligase parkin isoform 2
NM_013988 NP_054643 E3 ubiquitin-protein ligase parkin isoform 3



Homo sapiens (human) PARK2 NP_004553.2
Pan troglodytes (chimpanzee) PARK2 XP_001153913.1
Macaca mulatta (Rhesus monkey) LOC710899 XP_001099588.2
Canis lupus familiaris (dog) PARK2 XP_855152.2
Bos taurus (cattle) PARK2 NP_001185994.1
Mus musculus (house mouse) Park2 NP_057903.1
Rattus norvegicus (Norway rat) Park2 NP_064478.1
Gallus gallus (chicken) PARK2 XP_004935613.1
Danio rerio (zebrafish) park2 NP_001017635.1
Drosophila melanogaster (fruit fly) park NP_730601.1
Caenorhabditis elegans pdr-1 NP_499846.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PARK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PARK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu59479 XM_011535863 PREDICTED: Homo sapiens parkin RBR E3 ubiquitin protein ligase (PARK2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu59480 XM_011535864 PREDICTED: Homo sapiens parkin RBR E3 ubiquitin protein ligase (PARK2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu59481 XM_011535865 PREDICTED: Homo sapiens parkin RBR E3 ubiquitin protein ligase (PARK2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu59482 XM_011535866 PREDICTED: Homo sapiens parkin RBR E3 ubiquitin protein ligase (PARK2), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu59483 XM_011535867 PREDICTED: Homo sapiens parkin RBR E3 ubiquitin protein ligase (PARK2), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu26139
NM_004562 Homo sapiens parkin RBR E3 ubiquitin protein ligase (PARK2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $159.50
$319.00
OHu26861 NM_013987 Homo sapiens parkin RBR E3 ubiquitin protein ligase (PARK2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu26823 NM_013988 Homo sapiens parkin RBR E3 ubiquitin protein ligase (PARK2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $216.30
$309.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu59479
Clone ID Related Accession (Same CDS sequence) XM_011535863
Accession Version XM_011535863.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1395bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product E3 ubiquitin-protein ligase parkin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_025741.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGATAGTGT TTGTCAGGTT CAACTCCAGC CATGGTTTCC CAGTGGAGGT CGATTCTGAC 
ACCAGCATCT TCCAGCTCAA GGAGGTGGTT GCTAAGCGAC AGGGGGTTCC GGCTGACCAG
TTGCGTGTGA TTTTCGCAGG GAAGGAGCTG AGGAATGACT GGACTGTGCA GAATTGTGAC
CTGGATCAGC AGAGCATTGT TCACATTGTG CAGAGACCGT GGAGAAAAGG TCAAGAAATG
AATGCAACTG GAGGCGACGA CCCCAGAAAC GCGGCGGGAG GCTGTGAGCG GGAGCCCCAG
AGCTTGACTC GGGTGGACCT CAGCAGCTCA GTCCTCCCAG GAGACTCTGT GGGGCTGGCT
GTCATTCTGC ACACTGACAG CAGGAAGGAC TCACCACCAG CTGGAAGTCC AGGTAGATCA
ATCTACAACA GCTTTTATGT GTATTGCAAA GGCCCCTGTC AAAGAGTGCA GCCGGGAAAA
CTCAGGGTAC AGTGCAGCAC CTGCAGGCAG GCAACGCTCA CCTTGACCCA GGGTCCATCT
TGCTGGGATG ATGTTTTAAT TCCAAACCGG ATGAGTGGTG AATGCCAATC CCCACACTGC
CCTGGGACTA GTGCAGAATT TTTCTTTAAA TGTGGAGCAC ACCCCACCTC TGACAAGGAA
ACATCAGTAG CTTTGCACCT GATCGCAACA AATAGTCGGA ACATCACTTG CATTACGTGC
ACAGACGTCA GGAGCCCCGT CCTGGTTTTC CAGTGCAACT CCCGCCACGT GATTTGCTTA
GACTGTTTCC ACTTATACTG TGTGACAAGA CTCAATGATC GGCAGTTTGT TCACGACCCT
CAACTTGGCT ACTCCCTGCC TTGTGTGGCT GGCTGTCCCA ACTCCTTGAT TAAAGAGCTC
CATCACTTCA GGATTCTGGG AGAAGAGCAG TACAACCGGT ACCAGCAGTA TGGTGCAGAG
GAGTGTGTCC TGCAGATGGG GGGCGTGTTA TGCCCCCGCC CTGGCTGTGG AGCGGGGCTG
CTGCCGGAGC CTGACCAGAG GAAAGTCACC TGCGAAGGGG GCAATGGCCT GGGCTGTGGG
TTTGCCTTCT GCCGGGAATG TAAAGAAGCG TACCATGAAG GGGAGTGCAG TGCCGTATTT
GAAGCCTCAG GAACAACTAC TCAGGCCTAC AGAGTCGATG AAAGAGCCGC CGAGCAGGCT
CGTTGGGAAG CAGCCTCCAA AGAAACCATC AAGAAAACCA CCAAGCCCTG TCCCCGCTGC
CATGTACCAG TGGAAAAAAA TGGAGGCTGC ATGCACATGA AGTGTCCGCA GCCCCAGTGC
AGGCTCGAGT GGTGCTGGAA CTGTGGCTGC GAGTGGAACC GCGTCTGCAT GGGGGACCAC
TGGTTCGACG TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011534165.1
CDS135..1529
Misc Feature(1)135..350(+)
Misc Feature(2)141..350(+)
Misc Feature(3)258..260(+)
Misc Feature(4)1068..1256(+)
Misc Feature(5)1365..1490(+)
Translation

Target ORF information:

RefSeq Version XM_011535863
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens parkin RBR E3 ubiquitin protein ligase (PARK2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535863

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGATAGTGT TTGTCAGGTT CAACTCCAGC CATGGTTTCC CAGTGGAGGT CGATTCTGAC 
ACCAGCATCT TCCAGCTCAA GGAGGTGGTT GCTAAGCGAC AGGGGGTTCC GGCTGACCAG
TTGCGTGTGA TTTTCGCAGG GAAGGAGCTG AGGAATGACT GGACTGTGCA GAATTGTGAC
CTGGATCAGC AGAGCATTGT TCACATTGTG CAGAGACCGT GGAGAAAAGG TCAAGAAATG
AATGCAACTG GAGGCGACGA CCCCAGAAAC GCGGCGGGAG GCTGTGAGCG GGAGCCCCAG
AGCTTGACTC GGGTGGACCT CAGCAGCTCA GTCCTCCCAG GAGACTCTGT GGGGCTGGCT
GTCATTCTGC ACACTGACAG CAGGAAGGAC TCACCACCAG CTGGAAGTCC AGGTAGATCA
ATCTACAACA GCTTTTATGT GTATTGCAAA GGCCCCTGTC AAAGAGTGCA GCCGGGAAAA
CTCAGGGTAC AGTGCAGCAC CTGCAGGCAG GCAACGCTCA CCTTGACCCA GGGTCCATCT
TGCTGGGATG ATGTTTTAAT TCCAAACCGG ATGAGTGGTG AATGCCAATC CCCACACTGC
CCTGGGACTA GTGCAGAATT TTTCTTTAAA TGTGGAGCAC ACCCCACCTC TGACAAGGAA
ACATCAGTAG CTTTGCACCT GATCGCAACA AATAGTCGGA ACATCACTTG CATTACGTGC
ACAGACGTCA GGAGCCCCGT CCTGGTTTTC CAGTGCAACT CCCGCCACGT GATTTGCTTA
GACTGTTTCC ACTTATACTG TGTGACAAGA CTCAATGATC GGCAGTTTGT TCACGACCCT
CAACTTGGCT ACTCCCTGCC TTGTGTGGCT GGCTGTCCCA ACTCCTTGAT TAAAGAGCTC
CATCACTTCA GGATTCTGGG AGAAGAGCAG TACAACCGGT ACCAGCAGTA TGGTGCAGAG
GAGTGTGTCC TGCAGATGGG GGGCGTGTTA TGCCCCCGCC CTGGCTGTGG AGCGGGGCTG
CTGCCGGAGC CTGACCAGAG GAAAGTCACC TGCGAAGGGG GCAATGGCCT GGGCTGTGGG
TTTGCCTTCT GCCGGGAATG TAAAGAAGCG TACCATGAAG GGGAGTGCAG TGCCGTATTT
GAAGCCTCAG GAACAACTAC TCAGGCCTAC AGAGTCGATG AAAGAGCCGC CGAGCAGGCT
CGTTGGGAAG CAGCCTCCAA AGAAACCATC AAGAAAACCA CCAAGCCCTG TCCCCGCTGC
CATGTACCAG TGGAAAAAAA TGGAGGCTGC ATGCACATGA AGTGTCCGCA GCCCCAGTGC
AGGCTCGAGT GGTGCTGGAA CTGTGGCTGC GAGTGGAACC GCGTCTGCAT GGGGGACCAC
TGGTTCGACG TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59480
Clone ID Related Accession (Same CDS sequence) XM_011535864
Accession Version XM_011535864.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1107bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product E3 ubiquitin-protein ligase parkin isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_025741.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGATAGTGT TTGTCAGGTT CAACTCCAGC CATGGTTTCC CAGTGGAGGT CGATTCTGAC 
ACCAGCATCT TCCAGCTCAA GGAGGTGGTT GCTAAGCGAC AGGGGGTTCC GGCTGACCAG
TTGCGTGTGA TTTTCGCAGG GAAGGAGCTG AGGAATGACT GGACTGTGCA GAATTGTGAC
CTGGATCAGC AGAGCATTGT TCACATTGTG CAGAGACCGT GGAGAAAAGG TCAAGAAATG
AATGCAACTG GAGGCGACGA CCCCAGAAAC GCGGCGGGAG GCTGTGAGCG GGAGCCCCAG
AGCTTGACTC GGGTGGACCT CAGCAGCTCA GTCCTCCCAG GAGACTCTGT GGGGCTGGCT
GTCATTCTGC ACACTGACAG CAGGAAGGAC TCACCACCAG CTGGAAGTCC AGCAGGTAGA
TCAATCTACA ACAGCTTTTA TGTGTATTGC AAAGGCCCCT GTCAAAGAGT GCAGCCGGGA
AAACTCAGGG TACAGTGCAG CACCTGCAGG CAGGCAACGC TCACCTTGAC CCAGGGTCCA
TCTTGCTGGG ATGATGTTTT AATTCCAAAC CGGATGAGTG GTGAATGCCA ATCCCCACAC
TGCCCTGGGA CTAGTGCAGA ATTTTTCTTT AAATGTGGAG CACACCCCAC CTCTGACAAG
GAAACATCAG TAGCTTTGCA CCTGATCGCA ACAAATAGTC GGAACATCAC TTGCATTACG
TGCACAGACG TCAGGAGCCC CGTCCTGGTT TTCCAGTGCA ACTCCCGCCA CGTGATTTGC
TTAGACTGTT TCCACTTATA CTGTGTGACA AGACTCAATG ATCGGCAGTT TGTTCACGAC
CCTCAACTTG GCTACTCCCT GCCTTGTGTG GCTGGCTGTC CCAACTCCTT GATTAAAGAG
CTCCATCACT TCAGGATTCT GGGAGAAGAG CAGTACAACC GGTACCAGCA GTATGGTGCA
GAGGAGTGTG TCCTGCAGAT GGGGGGCGTG TTATGCCCCC GCCCTGGCTG TGGAGCGGGG
CTGCTGCCGG AGCCTGACCA GAGGAAAGTC ACCTGCGAAG GGGGCAATGG CCTGGGCTGT
GGGTATGGAC AACGAAGAAC AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011534166.1
CDS135..1241
Misc Feature(1)135..350(+)
Misc Feature(2)141..350(+)
Misc Feature(3)258..260(+)
Translation

Target ORF information:

RefSeq Version XM_011535864
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens parkin RBR E3 ubiquitin protein ligase (PARK2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535864

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGATAGTGT TTGTCAGGTT CAACTCCAGC CATGGTTTCC CAGTGGAGGT CGATTCTGAC 
ACCAGCATCT TCCAGCTCAA GGAGGTGGTT GCTAAGCGAC AGGGGGTTCC GGCTGACCAG
TTGCGTGTGA TTTTCGCAGG GAAGGAGCTG AGGAATGACT GGACTGTGCA GAATTGTGAC
CTGGATCAGC AGAGCATTGT TCACATTGTG CAGAGACCGT GGAGAAAAGG TCAAGAAATG
AATGCAACTG GAGGCGACGA CCCCAGAAAC GCGGCGGGAG GCTGTGAGCG GGAGCCCCAG
AGCTTGACTC GGGTGGACCT CAGCAGCTCA GTCCTCCCAG GAGACTCTGT GGGGCTGGCT
GTCATTCTGC ACACTGACAG CAGGAAGGAC TCACCACCAG CTGGAAGTCC AGCAGGTAGA
TCAATCTACA ACAGCTTTTA TGTGTATTGC AAAGGCCCCT GTCAAAGAGT GCAGCCGGGA
AAACTCAGGG TACAGTGCAG CACCTGCAGG CAGGCAACGC TCACCTTGAC CCAGGGTCCA
TCTTGCTGGG ATGATGTTTT AATTCCAAAC CGGATGAGTG GTGAATGCCA ATCCCCACAC
TGCCCTGGGA CTAGTGCAGA ATTTTTCTTT AAATGTGGAG CACACCCCAC CTCTGACAAG
GAAACATCAG TAGCTTTGCA CCTGATCGCA ACAAATAGTC GGAACATCAC TTGCATTACG
TGCACAGACG TCAGGAGCCC CGTCCTGGTT TTCCAGTGCA ACTCCCGCCA CGTGATTTGC
TTAGACTGTT TCCACTTATA CTGTGTGACA AGACTCAATG ATCGGCAGTT TGTTCACGAC
CCTCAACTTG GCTACTCCCT GCCTTGTGTG GCTGGCTGTC CCAACTCCTT GATTAAAGAG
CTCCATCACT TCAGGATTCT GGGAGAAGAG CAGTACAACC GGTACCAGCA GTATGGTGCA
GAGGAGTGTG TCCTGCAGAT GGGGGGCGTG TTATGCCCCC GCCCTGGCTG TGGAGCGGGG
CTGCTGCCGG AGCCTGACCA GAGGAAAGTC ACCTGCGAAG GGGGCAATGG CCTGGGCTGT
GGGTATGGAC AACGAAGAAC AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59481
Clone ID Related Accession (Same CDS sequence) XM_011535865
Accession Version XM_011535865.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1092bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product E3 ubiquitin-protein ligase parkin isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_025741.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGATAGTGT TTGTCAGGTT CAACTCCAGC CATGGTTTCC CAGTGGAGGT CGATTCTGAC 
ACCAGCATCT TCCAGCTCAA GGAGGTGGTT GCTAAGCGAC AGGGGGTTCC GGCTGACCAG
TTGCGTGTGA TTTTCGCAGG GAAGGAGCTG AGGAATGACT GGACTGTGCA GAATTGTGAC
CTGGATCAGC AGAGCATTGT TCACATTGTG CAGAGACCGT GGAGAAAAGG TCAAGAAATG
AATGCAACTG GAGGCGACGA CCCCAGAAAC GCGGCGGGAG GCTGTGAGCG GGAGCCCCAG
AGCTTGACTC GGGTGGACCT CAGCAGCTCA GTCCTCCCAG GAGACTCTGT GGGGCTGGCT
GTCATTCTGC ACACTGACAG CAGGAAGGAC TCACCACCAG CTGGAAGTCC AGCAGGTAGA
TCAATCTACA ACAGCTTTTA TGTGTATTGC AAAGGCCCCT GTCAAAGAGT GCAGCCGGGA
AAACTCAGGG TACAGTGCAG CACCTGCAGG CAGGCAACGC TCACCTTGAC CCAGGGTCCA
TCTTGCTGGG ATGATGTTTT AATTCCAAAC CGGATGAGTG GTGAATGCCA ATCCCCACAC
TGCCCTGGGA CTAGTGCAGA ATTTTTCTTT AAATGTGGAG CACACCCCAC CTCTGACAAG
GAAACATCAG TAGCTTTGCA CCTGATCGCA ACAAATAGTC GGAACATCAC TTGCATTACG
TGCACAGACG TCAGGAGCCC CGTCCTGGTT TTCCAGTGCA ACTCCCGCCA CGTGATTTGC
TTAGACTGTT TCCACTTATA CTGTGTGACA AGACTCAATG ATCGGCAGTT TGTTCACGAC
CCTCAACTTG GCTACTCCCT GCCTTGTGTG GCTGGCTGTC CCAACTCCTT GATTAAAGAG
CTCCATCACT TCAGGATTCT GGGAGAAGAG CAGTACAACC GGTACCAGCA GTATGGTGCA
GAGGAGTGTG TCCTGCAGAT GGGGGGCGTG TTATGCCCCC GCCCTGGCTG TGGAGCGGGG
CTGCTGCCGG AGCCTGACCA GAGGAAAGTC ACCTGCGAAG GGGGCAATGG CCTGGGCTGT
GGGGTCAGTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011534167.1
CDS135..1226
Misc Feature(1)135..350(+)
Misc Feature(2)141..350(+)
Misc Feature(3)258..260(+)
Translation

Target ORF information:

RefSeq Version XM_011535865
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens parkin RBR E3 ubiquitin protein ligase (PARK2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535865

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGATAGTGT TTGTCAGGTT CAACTCCAGC CATGGTTTCC CAGTGGAGGT CGATTCTGAC 
ACCAGCATCT TCCAGCTCAA GGAGGTGGTT GCTAAGCGAC AGGGGGTTCC GGCTGACCAG
TTGCGTGTGA TTTTCGCAGG GAAGGAGCTG AGGAATGACT GGACTGTGCA GAATTGTGAC
CTGGATCAGC AGAGCATTGT TCACATTGTG CAGAGACCGT GGAGAAAAGG TCAAGAAATG
AATGCAACTG GAGGCGACGA CCCCAGAAAC GCGGCGGGAG GCTGTGAGCG GGAGCCCCAG
AGCTTGACTC GGGTGGACCT CAGCAGCTCA GTCCTCCCAG GAGACTCTGT GGGGCTGGCT
GTCATTCTGC ACACTGACAG CAGGAAGGAC TCACCACCAG CTGGAAGTCC AGCAGGTAGA
TCAATCTACA ACAGCTTTTA TGTGTATTGC AAAGGCCCCT GTCAAAGAGT GCAGCCGGGA
AAACTCAGGG TACAGTGCAG CACCTGCAGG CAGGCAACGC TCACCTTGAC CCAGGGTCCA
TCTTGCTGGG ATGATGTTTT AATTCCAAAC CGGATGAGTG GTGAATGCCA ATCCCCACAC
TGCCCTGGGA CTAGTGCAGA ATTTTTCTTT AAATGTGGAG CACACCCCAC CTCTGACAAG
GAAACATCAG TAGCTTTGCA CCTGATCGCA ACAAATAGTC GGAACATCAC TTGCATTACG
TGCACAGACG TCAGGAGCCC CGTCCTGGTT TTCCAGTGCA ACTCCCGCCA CGTGATTTGC
TTAGACTGTT TCCACTTATA CTGTGTGACA AGACTCAATG ATCGGCAGTT TGTTCACGAC
CCTCAACTTG GCTACTCCCT GCCTTGTGTG GCTGGCTGTC CCAACTCCTT GATTAAAGAG
CTCCATCACT TCAGGATTCT GGGAGAAGAG CAGTACAACC GGTACCAGCA GTATGGTGCA
GAGGAGTGTG TCCTGCAGAT GGGGGGCGTG TTATGCCCCC GCCCTGGCTG TGGAGCGGGG
CTGCTGCCGG AGCCTGACCA GAGGAAAGTC ACCTGCGAAG GGGGCAATGG CCTGGGCTGT
GGGGTCAGTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59482
Clone ID Related Accession (Same CDS sequence) XM_011535866
Accession Version XM_011535866.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 765bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product E3 ubiquitin-protein ligase parkin isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_025741.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGATAGTGT TTGTCAGGTT CAACTCCAGC CATGGTTTCC CAGTGGAGGT CGATTCTGAC 
ACCAGCATCT TCCAGCTCAA GGAGGTGGTT GCTAAGCGAC AGGGGGTTCC GGCTGACCAG
TTGCGTGTGA TTTTCGCAGG GAAGGAGCTG AGGAATGACT GGACTGTGCA GAATTGTGAC
CTGGATCAGC AGAGCATTGT TCACATTGTG CAGAGACCGT GGAGAAAAGG TCAAGAAATG
AATGCAACTG GAGGCGACGA CCCCAGAAAC GCGGCGGGAG GCTGTGAGCG GGAGCCCCAG
AGCTTGACTC GGGTGGACCT CAGCAGCTCA GTCCTCCCAG GAGACTCTGT GGGGCTGGCT
GTCATTCTGC ACACTGACAG CAGGAAGGAC TCACCACCAG CTGGAAGTCC AGCAGGTAGA
TCAATCTACA ACAGCTTTTA TGTGTATTGC AAAGGCCCCT GTCAAAGAGT GCAGCCGGGA
AAACTCAGGG TACAGTGCAG CACCTGCAGG CAGGCAACGC TCACCTTGAC CCAGGGTCCA
TCTTGCTGGG ATGATGTTTT AATTCCAAAC CGGATGAGTG GTGAATGCCA ATCCCCACAC
TGCCCTGGGA CTAGTGCAGA ATTTTTCTTT AAATGTGGAG CACACCCCAC CTCTGACAAG
GAAACATCAG TAGCTTTGCA CCTGATCGCA ACAAATAGTC GGAACATCAC TTGCATTACG
TGCACAGACG TCAGAATTAT TTTCAGCTTC GCGTCAACTT CATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011534168.1
CDS135..899
Misc Feature(1)135..350(+)
Misc Feature(2)141..350(+)
Misc Feature(3)258..260(+)
Translation

Target ORF information:

RefSeq Version XM_011535866
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens parkin RBR E3 ubiquitin protein ligase (PARK2), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535866

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGATAGTGT TTGTCAGGTT CAACTCCAGC CATGGTTTCC CAGTGGAGGT CGATTCTGAC 
ACCAGCATCT TCCAGCTCAA GGAGGTGGTT GCTAAGCGAC AGGGGGTTCC GGCTGACCAG
TTGCGTGTGA TTTTCGCAGG GAAGGAGCTG AGGAATGACT GGACTGTGCA GAATTGTGAC
CTGGATCAGC AGAGCATTGT TCACATTGTG CAGAGACCGT GGAGAAAAGG TCAAGAAATG
AATGCAACTG GAGGCGACGA CCCCAGAAAC GCGGCGGGAG GCTGTGAGCG GGAGCCCCAG
AGCTTGACTC GGGTGGACCT CAGCAGCTCA GTCCTCCCAG GAGACTCTGT GGGGCTGGCT
GTCATTCTGC ACACTGACAG CAGGAAGGAC TCACCACCAG CTGGAAGTCC AGCAGGTAGA
TCAATCTACA ACAGCTTTTA TGTGTATTGC AAAGGCCCCT GTCAAAGAGT GCAGCCGGGA
AAACTCAGGG TACAGTGCAG CACCTGCAGG CAGGCAACGC TCACCTTGAC CCAGGGTCCA
TCTTGCTGGG ATGATGTTTT AATTCCAAAC CGGATGAGTG GTGAATGCCA ATCCCCACAC
TGCCCTGGGA CTAGTGCAGA ATTTTTCTTT AAATGTGGAG CACACCCCAC CTCTGACAAG
GAAACATCAG TAGCTTTGCA CCTGATCGCA ACAAATAGTC GGAACATCAC TTGCATTACG
TGCACAGACG TCAGAATTAT TTTCAGCTTC GCGTCAACTT CATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59483
Clone ID Related Accession (Same CDS sequence) XM_011535867
Accession Version XM_011535867.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 750bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product E3 ubiquitin-protein ligase parkin isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_025741.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGATAGTGT TTGTCAGGTT CAACTCCAGC CATGGTTTCC CAGTGGAGGT CGATTCTGAC 
ACCAGCATCT TCCAGCTCAA GGAGGTGGTT GCTAAGCGAC AGGGGGTTCC GGCTGACCAG
TTGCGTGTGA TTTTCGCAGG GAAGGAGCTG AGGAATGACT GGACTGTGCA GAATTGTGAC
CTGGATCAGC AGAGCATTGT TCACATTGTG CAGAGACCGT GGAGAAAAGG TCAAGAAATG
AATGCAACTG GAGGCGACGA CCCCAGAAAC GCGGCGGGAG GCTGTGAGCG GGAGCCCCAG
AGCTTGACTC GGGTGGACCT CAGCAGCTCA GTCCTCCCAG GAGACTCTGT GGGGCTGGCT
GTCATTCTGC ACACTGACAG CAGGAAGGAC TCACCACCAG CTGGAAGTCC AGCAGGTAGA
TCAATCTACA ACAGCTTTTA TGTGTATTGC AAAGGCCCCT GTCAAAGAGT GCAGCCGGGA
AAACTCAGGG TACAGTGCAG CACCTGCAGG CAGGCAACGC TCACCTTGAC CCAGGGTCCA
TCTTGCTGGG ATGATGTTTT AATTCCAAAC CGGATGAGTG GTGAATGCCA ATCCCCACAC
TGCCCTGGGA CTAGTGCAGA ATTTTTCTTT AAATGTGGAG CACACCCCAC CTCTGACAAG
GAAACATCAG TAGCTTTGCA CCTGATCGCA ACAAATAGTC GGAACATCAC TTGCATTACG
TGCACAGACG TCAGAAATAA TTCACAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011534169.1
CDS135..884
Misc Feature(1)135..350(+)
Misc Feature(2)141..350(+)
Misc Feature(3)258..260(+)
Translation

Target ORF information:

RefSeq Version XM_011535867
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens parkin RBR E3 ubiquitin protein ligase (PARK2), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535867

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGATAGTGT TTGTCAGGTT CAACTCCAGC CATGGTTTCC CAGTGGAGGT CGATTCTGAC 
ACCAGCATCT TCCAGCTCAA GGAGGTGGTT GCTAAGCGAC AGGGGGTTCC GGCTGACCAG
TTGCGTGTGA TTTTCGCAGG GAAGGAGCTG AGGAATGACT GGACTGTGCA GAATTGTGAC
CTGGATCAGC AGAGCATTGT TCACATTGTG CAGAGACCGT GGAGAAAAGG TCAAGAAATG
AATGCAACTG GAGGCGACGA CCCCAGAAAC GCGGCGGGAG GCTGTGAGCG GGAGCCCCAG
AGCTTGACTC GGGTGGACCT CAGCAGCTCA GTCCTCCCAG GAGACTCTGT GGGGCTGGCT
GTCATTCTGC ACACTGACAG CAGGAAGGAC TCACCACCAG CTGGAAGTCC AGCAGGTAGA
TCAATCTACA ACAGCTTTTA TGTGTATTGC AAAGGCCCCT GTCAAAGAGT GCAGCCGGGA
AAACTCAGGG TACAGTGCAG CACCTGCAGG CAGGCAACGC TCACCTTGAC CCAGGGTCCA
TCTTGCTGGG ATGATGTTTT AATTCCAAAC CGGATGAGTG GTGAATGCCA ATCCCCACAC
TGCCCTGGGA CTAGTGCAGA ATTTTTCTTT AAATGTGGAG CACACCCCAC CTCTGACAAG
GAAACATCAG TAGCTTTGCA CCTGATCGCA ACAAATAGTC GGAACATCAC TTGCATTACG
TGCACAGACG TCAGAAATAA TTCACAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26139
Clone ID Related Accession (Same CDS sequence) NM_004562
Accession Version NM_004562.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1398bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product E3 ubiquitin-protein ligase parkin isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB023187.1, AK292590.1 and AL132982.12. This sequence is a reference standard in the RefSeqGene project. On Mar 12, 2008 this sequence version replaced gi:4758883. Transcript Variant: Transcript variant 1 represents the predominant and full-length form of this gene. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB009973.1, AB245403.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGATAGTGT TTGTCAGGTT CAACTCCAGC CATGGTTTCC CAGTGGAGGT CGATTCTGAC 
ACCAGCATCT TCCAGCTCAA GGAGGTGGTT GCTAAGCGAC AGGGGGTTCC GGCTGACCAG
TTGCGTGTGA TTTTCGCAGG GAAGGAGCTG AGGAATGACT GGACTGTGCA GAATTGTGAC
CTGGATCAGC AGAGCATTGT TCACATTGTG CAGAGACCGT GGAGAAAAGG TCAAGAAATG
AATGCAACTG GAGGCGACGA CCCCAGAAAC GCGGCGGGAG GCTGTGAGCG GGAGCCCCAG
AGCTTGACTC GGGTGGACCT CAGCAGCTCA GTCCTCCCAG GAGACTCTGT GGGGCTGGCT
GTCATTCTGC ACACTGACAG CAGGAAGGAC TCACCACCAG CTGGAAGTCC AGCAGGTAGA
TCAATCTACA ACAGCTTTTA TGTGTATTGC AAAGGCCCCT GTCAAAGAGT GCAGCCGGGA
AAACTCAGGG TACAGTGCAG CACCTGCAGG CAGGCAACGC TCACCTTGAC CCAGGGTCCA
TCTTGCTGGG ATGATGTTTT AATTCCAAAC CGGATGAGTG GTGAATGCCA ATCCCCACAC
TGCCCTGGGA CTAGTGCAGA ATTTTTCTTT AAATGTGGAG CACACCCCAC CTCTGACAAG
GAAACATCAG TAGCTTTGCA CCTGATCGCA ACAAATAGTC GGAACATCAC TTGCATTACG
TGCACAGACG TCAGGAGCCC CGTCCTGGTT TTCCAGTGCA ACTCCCGCCA CGTGATTTGC
TTAGACTGTT TCCACTTATA CTGTGTGACA AGACTCAATG ATCGGCAGTT TGTTCACGAC
CCTCAACTTG GCTACTCCCT GCCTTGTGTG GCTGGCTGTC CCAACTCCTT GATTAAAGAG
CTCCATCACT TCAGGATTCT GGGAGAAGAG CAGTACAACC GGTACCAGCA GTATGGTGCA
GAGGAGTGTG TCCTGCAGAT GGGGGGCGTG TTATGCCCCC GCCCTGGCTG TGGAGCGGGG
CTGCTGCCGG AGCCTGACCA GAGGAAAGTC ACCTGCGAAG GGGGCAATGG CCTGGGCTGT
GGGTTTGCCT TCTGCCGGGA ATGTAAAGAA GCGTACCATG AAGGGGAGTG CAGTGCCGTA
TTTGAAGCCT CAGGAACAAC TACTCAGGCC TACAGAGTCG ATGAAAGAGC CGCCGAGCAG
GCTCGTTGGG AAGCAGCCTC CAAAGAAACC ATCAAGAAAA CCACCAAGCC CTGTCCCCGC
TGCCATGTAC CAGTGGAAAA AAATGGAGGC TGCATGCACA TGAAGTGTCC GCAGCCCCAG
TGCAGGCTCG AGTGGTGCTG GAACTGTGGC TGCGAGTGGA ACCGCGTCTG CATGGGGGAC
CACTGGTTCG ACGTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004553.2
CDS135..1532
Misc Feature(1)6..8(+)
Misc Feature(2)135..350(+)
Misc Feature(3)141..350(+)
Misc Feature(4)258..260(+)
Misc Feature(5)744..848(+)
Misc Feature(6)903..1013(+)
Misc Feature(7)1071..1259(+)
Misc Feature(8)1266..1364(+)
Misc Feature(9)1368..1493(+)
Exon (1)1..141
Gene:PARK2
Gene Synonym:
Exon (2)142..305
Gene:PARK2
Gene Synonym:
Exon (3)306..546
Gene:PARK2
Gene Synonym:
Exon (4)547..668
Gene:PARK2
Gene Synonym:
Exon (5)669..752
Gene:PARK2
Gene Synonym:
Exon (6)753..868
Gene:PARK2
Gene Synonym:
Exon (7)869..1005
Gene:PARK2
Gene Synonym:
Exon (8)1006..1067
Gene:PARK2
Gene Synonym:
Exon (9)1068..1217
Gene:PARK2
Gene Synonym:
Exon (10)1218..1301
Gene:PARK2
Gene Synonym:
Exon (11)1302..1419
Gene:PARK2
Gene Synonym:
Exon (12)1420..4073
Gene:PARK2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_004562
Organism Homo sapiens (human)
Definition Homo sapiens parkin RBR E3 ubiquitin protein ligase (PARK2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004562

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGATAGTGT TTGTCAGGTT CAACTCCAGC CATGGTTTCC CAGTGGAGGT CGATTCTGAC 
ACCAGCATCT TCCAGCTCAA GGAGGTGGTT GCTAAGCGAC AGGGGGTTCC GGCTGACCAG
TTGCGTGTGA TTTTCGCAGG GAAGGAGCTG AGGAATGACT GGACTGTGCA GAATTGTGAC
CTGGATCAGC AGAGCATTGT TCACATTGTG CAGAGACCGT GGAGAAAAGG TCAAGAAATG
AATGCAACTG GAGGCGACGA CCCCAGAAAC GCGGCGGGAG GCTGTGAGCG GGAGCCCCAG
AGCTTGACTC GGGTGGACCT CAGCAGCTCA GTCCTCCCAG GAGACTCTGT GGGGCTGGCT
GTCATTCTGC ACACTGACAG CAGGAAGGAC TCACCACCAG CTGGAAGTCC AGCAGGTAGA
TCAATCTACA ACAGCTTTTA TGTGTATTGC AAAGGCCCCT GTCAAAGAGT GCAGCCGGGA
AAACTCAGGG TACAGTGCAG CACCTGCAGG CAGGCAACGC TCACCTTGAC CCAGGGTCCA
TCTTGCTGGG ATGATGTTTT AATTCCAAAC CGGATGAGTG GTGAATGCCA ATCCCCACAC
TGCCCTGGGA CTAGTGCAGA ATTTTTCTTT AAATGTGGAG CACACCCCAC CTCTGACAAG
GAAACATCAG TAGCTTTGCA CCTGATCGCA ACAAATAGTC GGAACATCAC TTGCATTACG
TGCACAGACG TCAGGAGCCC CGTCCTGGTT TTCCAGTGCA ACTCCCGCCA CGTGATTTGC
TTAGACTGTT TCCACTTATA CTGTGTGACA AGACTCAATG ATCGGCAGTT TGTTCACGAC
CCTCAACTTG GCTACTCCCT GCCTTGTGTG GCTGGCTGTC CCAACTCCTT GATTAAAGAG
CTCCATCACT TCAGGATTCT GGGAGAAGAG CAGTACAACC GGTACCAGCA GTATGGTGCA
GAGGAGTGTG TCCTGCAGAT GGGGGGCGTG TTATGCCCCC GCCCTGGCTG TGGAGCGGGG
CTGCTGCCGG AGCCTGACCA GAGGAAAGTC ACCTGCGAAG GGGGCAATGG CCTGGGCTGT
GGGTTTGCCT TCTGCCGGGA ATGTAAAGAA GCGTACCATG AAGGGGAGTG CAGTGCCGTA
TTTGAAGCCT CAGGAACAAC TACTCAGGCC TACAGAGTCG ATGAAAGAGC CGCCGAGCAG
GCTCGTTGGG AAGCAGCCTC CAAAGAAACC ATCAAGAAAA CCACCAAGCC CTGTCCCCGC
TGCCATGTAC CAGTGGAAAA AAATGGAGGC TGCATGCACA TGAAGTGTCC GCAGCCCCAG
TGCAGGCTCG AGTGGTGCTG GAACTGTGGC TGCGAGTGGA ACCGCGTCTG CATGGGGGAC
CACTGGTTCG ACGTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26861
Clone ID Related Accession (Same CDS sequence) NM_013987
Accession Version NM_013987.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1314bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product E3 ubiquitin-protein ligase parkin isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB023187.1, AK292590.1 and AL132982.12. On Mar 12, 2008 this sequence version replaced gi:7669537. Transcript Variant: Transcript variant 2 lacks exons 5 which is present in the full-length variant 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGATAGTGT TTGTCAGGTT CAACTCCAGC CATGGTTTCC CAGTGGAGGT CGATTCTGAC 
ACCAGCATCT TCCAGCTCAA GGAGGTGGTT GCTAAGCGAC AGGGGGTTCC GGCTGACCAG
TTGCGTGTGA TTTTCGCAGG GAAGGAGCTG AGGAATGACT GGACTGTGCA GAATTGTGAC
CTGGATCAGC AGAGCATTGT TCACATTGTG CAGAGACCGT GGAGAAAAGG TCAAGAAATG
AATGCAACTG GAGGCGACGA CCCCAGAAAC GCGGCGGGAG GCTGTGAGCG GGAGCCCCAG
AGCTTGACTC GGGTGGACCT CAGCAGCTCA GTCCTCCCAG GAGACTCTGT GGGGCTGGCT
GTCATTCTGC ACACTGACAG CAGGAAGGAC TCACCACCAG CTGGAAGTCC AGCAGGTAGA
TCAATCTACA ACAGCTTTTA TGTGTATTGC AAAGGCCCCT GTCAAAGAGT GCAGCCGGGA
AAACTCAGGG TACAGTGCAG CACCTGCAGG CAGGCAACGC TCACCTTGAC CCAGGAATTT
TTCTTTAAAT GTGGAGCACA CCCCACCTCT GACAAGGAAA CATCAGTAGC TTTGCACCTG
ATCGCAACAA ATAGTCGGAA CATCACTTGC ATTACGTGCA CAGACGTCAG GAGCCCCGTC
CTGGTTTTCC AGTGCAACTC CCGCCACGTG ATTTGCTTAG ACTGTTTCCA CTTATACTGT
GTGACAAGAC TCAATGATCG GCAGTTTGTT CACGACCCTC AACTTGGCTA CTCCCTGCCT
TGTGTGGCTG GCTGTCCCAA CTCCTTGATT AAAGAGCTCC ATCACTTCAG GATTCTGGGA
GAAGAGCAGT ACAACCGGTA CCAGCAGTAT GGTGCAGAGG AGTGTGTCCT GCAGATGGGG
GGCGTGTTAT GCCCCCGCCC TGGCTGTGGA GCGGGGCTGC TGCCGGAGCC TGACCAGAGG
AAAGTCACCT GCGAAGGGGG CAATGGCCTG GGCTGTGGGT TTGCCTTCTG CCGGGAATGT
AAAGAAGCGT ACCATGAAGG GGAGTGCAGT GCCGTATTTG AAGCCTCAGG AACAACTACT
CAGGCCTACA GAGTCGATGA AAGAGCCGCC GAGCAGGCTC GTTGGGAAGC AGCCTCCAAA
GAAACCATCA AGAAAACCAC CAAGCCCTGT CCCCGCTGCC ATGTACCAGT GGAAAAAAAT
GGAGGCTGCA TGCACATGAA GTGTCCGCAG CCCCAGTGCA GGCTCGAGTG GTGCTGGAAC
TGTGGCTGCG AGTGGAACCG CGTCTGCATG GGGGACCACT GGTTCGACGT GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_054642.2
CDS135..1448
Misc Feature(1)6..8(+)
Misc Feature(2)135..350(+)
Misc Feature(3)141..350(+)
Misc Feature(4)258..260(+)
Misc Feature(5)987..1175(+)
Misc Feature(6)1284..1409(+)
Exon (1)1..141
Gene:PARK2
Gene Synonym:
Exon (2)142..305
Gene:PARK2
Gene Synonym:
Exon (3)306..546
Gene:PARK2
Gene Synonym:
Exon (4)547..668
Gene:PARK2
Gene Synonym:
Exon (5)669..784
Gene:PARK2
Gene Synonym:
Exon (6)785..921
Gene:PARK2
Gene Synonym:
Exon (7)922..983
Gene:PARK2
Gene Synonym:
Exon (8)984..1133
Gene:PARK2
Gene Synonym:
Exon (9)1134..1217
Gene:PARK2
Gene Synonym:
Exon (10)1218..1335
Gene:PARK2
Gene Synonym:
Exon (11)1336..3989
Gene:PARK2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_013987
Organism Homo sapiens (human)
Definition Homo sapiens parkin RBR E3 ubiquitin protein ligase (PARK2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_013987

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGATAGTGT TTGTCAGGTT CAACTCCAGC CATGGTTTCC CAGTGGAGGT CGATTCTGAC 
ACCAGCATCT TCCAGCTCAA GGAGGTGGTT GCTAAGCGAC AGGGGGTTCC GGCTGACCAG
TTGCGTGTGA TTTTCGCAGG GAAGGAGCTG AGGAATGACT GGACTGTGCA GAATTGTGAC
CTGGATCAGC AGAGCATTGT TCACATTGTG CAGAGACCGT GGAGAAAAGG TCAAGAAATG
AATGCAACTG GAGGCGACGA CCCCAGAAAC GCGGCGGGAG GCTGTGAGCG GGAGCCCCAG
AGCTTGACTC GGGTGGACCT CAGCAGCTCA GTCCTCCCAG GAGACTCTGT GGGGCTGGCT
GTCATTCTGC ACACTGACAG CAGGAAGGAC TCACCACCAG CTGGAAGTCC AGCAGGTAGA
TCAATCTACA ACAGCTTTTA TGTGTATTGC AAAGGCCCCT GTCAAAGAGT GCAGCCGGGA
AAACTCAGGG TACAGTGCAG CACCTGCAGG CAGGCAACGC TCACCTTGAC CCAGGAATTT
TTCTTTAAAT GTGGAGCACA CCCCACCTCT GACAAGGAAA CATCAGTAGC TTTGCACCTG
ATCGCAACAA ATAGTCGGAA CATCACTTGC ATTACGTGCA CAGACGTCAG GAGCCCCGTC
CTGGTTTTCC AGTGCAACTC CCGCCACGTG ATTTGCTTAG ACTGTTTCCA CTTATACTGT
GTGACAAGAC TCAATGATCG GCAGTTTGTT CACGACCCTC AACTTGGCTA CTCCCTGCCT
TGTGTGGCTG GCTGTCCCAA CTCCTTGATT AAAGAGCTCC ATCACTTCAG GATTCTGGGA
GAAGAGCAGT ACAACCGGTA CCAGCAGTAT GGTGCAGAGG AGTGTGTCCT GCAGATGGGG
GGCGTGTTAT GCCCCCGCCC TGGCTGTGGA GCGGGGCTGC TGCCGGAGCC TGACCAGAGG
AAAGTCACCT GCGAAGGGGG CAATGGCCTG GGCTGTGGGT TTGCCTTCTG CCGGGAATGT
AAAGAAGCGT ACCATGAAGG GGAGTGCAGT GCCGTATTTG AAGCCTCAGG AACAACTACT
CAGGCCTACA GAGTCGATGA AAGAGCCGCC GAGCAGGCTC GTTGGGAAGC AGCCTCCAAA
GAAACCATCA AGAAAACCAC CAAGCCCTGT CCCCGCTGCC ATGTACCAGT GGAAAAAAAT
GGAGGCTGCA TGCACATGAA GTGTCCGCAG CCCCAGTGCA GGCTCGAGTG GTGCTGGAAC
TGTGGCTGCG AGTGGAACCG CGTCTGCATG GGGGACCACT GGTTCGACGT GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26823
Clone ID Related Accession (Same CDS sequence) NM_013988
Accession Version NM_013988.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 951bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product E3 ubiquitin-protein ligase parkin isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB023187.1, AK292590.1 and AL132982.12. On Mar 12, 2008 this sequence version replaced gi:7669539. Transcript Variant: Transcript variant 3 lacks exons 3 to 5 present in the full-length transcript variant 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGATAGTGT TTGTCAGGTT CAACTCCAGC CATGGTTTCC CAGTGGAGGT CGATTCTGAC 
ACCAGCATCT TCCAGCTCAA GGAGGTGGTT GCTAAGCGAC AGGGGGTTCC GGCTGACCAG
TTGCGTGTGA TTTTCGCAGG GAAGGAGCTG AGGAATGACT GGACTGTGCA GGAATTTTTC
TTTAAATGTG GAGCACACCC CACCTCTGAC AAGGAAACAT CAGTAGCTTT GCACCTGATC
GCAACAAATA GTCGGAACAT CACTTGCATT ACGTGCACAG ACGTCAGGAG CCCCGTCCTG
GTTTTCCAGT GCAACTCCCG CCACGTGATT TGCTTAGACT GTTTCCACTT ATACTGTGTG
ACAAGACTCA ATGATCGGCA GTTTGTTCAC GACCCTCAAC TTGGCTACTC CCTGCCTTGT
GTGGCTGGCT GTCCCAACTC CTTGATTAAA GAGCTCCATC ACTTCAGGAT TCTGGGAGAA
GAGCAGTACA ACCGGTACCA GCAGTATGGT GCAGAGGAGT GTGTCCTGCA GATGGGGGGC
GTGTTATGCC CCCGCCCTGG CTGTGGAGCG GGGCTGCTGC CGGAGCCTGA CCAGAGGAAA
GTCACCTGCG AAGGGGGCAA TGGCCTGGGC TGTGGGTTTG CCTTCTGCCG GGAATGTAAA
GAAGCGTACC ATGAAGGGGA GTGCAGTGCC GTATTTGAAG CCTCAGGAAC AACTACTCAG
GCCTACAGAG TCGATGAAAG AGCCGCCGAG CAGGCTCGTT GGGAAGCAGC CTCCAAAGAA
ACCATCAAGA AAACCACCAA GCCCTGTCCC CGCTGCCATG TACCAGTGGA AAAAAATGGA
GGCTGCATGC ACATGAAGTG TCCGCAGCCC CAGTGCAGGC TCGAGTGGTG CTGGAACTGT
GGCTGCGAGT GGAACCGCGT CTGCATGGGG GACCACTGGT TCGACGTGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_054643.2
CDS135..1085
Misc Feature(1)6..8(+)
Misc Feature(2)135..>308(+)
Misc Feature(3)141..380(+)
Misc Feature(4)258..260(+)
Misc Feature(5)624..812(+)
Misc Feature(6)921..1046(+)
Exon (1)1..141
Gene:PARK2
Gene Synonym:
Exon (2)142..305
Gene:PARK2
Gene Synonym:
Exon (3)306..421
Gene:PARK2
Gene Synonym:
Exon (4)422..558
Gene:PARK2
Gene Synonym:
Exon (5)559..620
Gene:PARK2
Gene Synonym:
Exon (6)621..770
Gene:PARK2
Gene Synonym:
Exon (7)771..854
Gene:PARK2
Gene Synonym:
Exon (8)855..972
Gene:PARK2
Gene Synonym:
Exon (9)973..3626
Gene:PARK2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_013988
Organism Homo sapiens (human)
Definition Homo sapiens parkin RBR E3 ubiquitin protein ligase (PARK2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_013988

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGATAGTGT TTGTCAGGTT CAACTCCAGC CATGGTTTCC CAGTGGAGGT CGATTCTGAC 
ACCAGCATCT TCCAGCTCAA GGAGGTGGTT GCTAAGCGAC AGGGGGTTCC GGCTGACCAG
TTGCGTGTGA TTTTCGCAGG GAAGGAGCTG AGGAATGACT GGACTGTGCA GGAATTTTTC
TTTAAATGTG GAGCACACCC CACCTCTGAC AAGGAAACAT CAGTAGCTTT GCACCTGATC
GCAACAAATA GTCGGAACAT CACTTGCATT ACGTGCACAG ACGTCAGGAG CCCCGTCCTG
GTTTTCCAGT GCAACTCCCG CCACGTGATT TGCTTAGACT GTTTCCACTT ATACTGTGTG
ACAAGACTCA ATGATCGGCA GTTTGTTCAC GACCCTCAAC TTGGCTACTC CCTGCCTTGT
GTGGCTGGCT GTCCCAACTC CTTGATTAAA GAGCTCCATC ACTTCAGGAT TCTGGGAGAA
GAGCAGTACA ACCGGTACCA GCAGTATGGT GCAGAGGAGT GTGTCCTGCA GATGGGGGGC
GTGTTATGCC CCCGCCCTGG CTGTGGAGCG GGGCTGCTGC CGGAGCCTGA CCAGAGGAAA
GTCACCTGCG AAGGGGGCAA TGGCCTGGGC TGTGGGTTTG CCTTCTGCCG GGAATGTAAA
GAAGCGTACC ATGAAGGGGA GTGCAGTGCC GTATTTGAAG CCTCAGGAAC AACTACTCAG
GCCTACAGAG TCGATGAAAG AGCCGCCGAG CAGGCTCGTT GGGAAGCAGC CTCCAAAGAA
ACCATCAAGA AAACCACCAA GCCCTGTCCC CGCTGCCATG TACCAGTGGA AAAAAATGGA
GGCTGCATGC ACATGAAGTG TCCGCAGCCC CAGTGCAGGC TCGAGTGGTG CTGGAACTGT
GGCTGCGAGT GGAACCGCGT CTGCATGGGG GACCACTGGT TCGACGTGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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