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IRAK4 interleukin-1 receptor-associated kinase 4 [Homo sapiens (human)]

Gene Symbol IRAK4
Entrez Gene ID 51135
Full Name interleukin-1 receptor-associated kinase 4
Synonyms IPD1, IRAK-4, NY-REN-64, REN64
General protein information
Preferred Names
interleukin-1 receptor-associated kinase 4
Names
interleukin-1 receptor-associated kinase 4
renal carcinoma antigen NY-REN-64
NP_001107654.1
NP_001138728.1
NP_001138729.1
NP_001138730.1
NP_057207.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q12

Summary This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]. lac of sum
Disorder MIM:

606883

Disorder Html: IRAK4 deficiency, 607676 (3); Invasive pneumococcal disease,
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu15137 XM_011538431 PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu15137 XM_006719438 PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu15137 XM_011538432 PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu15137 XM_011538433 PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu15137 XM_005268943 PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu15137 XM_005268945 PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu15137 XM_005268946 PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu15137 XM_005268944 PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu14924 XM_005268948 PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu14924 XM_005268947 PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu14924 XM_006719439 PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu14924 XM_005268949 PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu46611 XM_006719440 PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu46611 XM_006719442 PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu46611 XM_006719443 PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu46611 XM_006719441 PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu15137 NM_001114182 Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu15137 NM_016123 Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu14924 NM_001145256 Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu14924 NM_001145257 Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu14924 NM_001145258 Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu15137D
Sequence Information ORF Nucleotide Sequence (Length: 1383bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product interleukin-1 receptor-associated kinase 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAACAAAC CCATAACACC ATCAACATAT GTGCGCTGCC TCAATGTTGG ACTAATTAGG 
AAGCTGTCAG ATTTTATTGA TCCTCAAGAA GGATGGAAGA AGTTAGCTGT AGCTATTAAA
AAACCATCTG GTGATGATAG ATACAATCAG TTTCACATAA GGAGATTTGA AGCATTACTT
CAAACTGGAA AAAGTCCCAC TTCTGAATTA CTGTTTGACT GGGGCACCAC AAATTGCACA
GTTGGTGATC TTGTGGATCT TTTGATCCAA AATGAATTTT TTGCTCCTGC GAGTCTTTTG
CTCCCAGATG CTGTTCCCAA AACTGCTAAT ACACTACCTT CTAAAGAAGC TATAACAGTT
CAGCAAAAAC AGATGCCTTT CTGTGACAAA GACAGGACAT TGATGACACC TGTGCAGAAT
CTTGAACAAA GCTATATGCC ACCTGACTCC TCAAGTCCAG AAAATAAAAG TTTAGAAGTT
AGTGATACAC GTTTTCACAG TTTTTCATTT TATGAATTGA AGAATGTCAC AAATAACTTT
GATGAACGAC CCATTTCTGT TGGTGGTAAT AAAATGGGAG AGGGAGGATT TGGAGTTGTA
TATAAAGGCT ACGTAAATAA CACAACTGTG GCAGTGAAGA AGCTTGCAGC AATGGTTGAC
ATTACTACTG AAGAACTGAA ACAGCAGTTT GATCAAGAAA TAAAAGTAAT GGCAAAGTGT
CAACATGAAA ACTTAGTAGA ACTACTTGGT TTCTCAAGTG ATGGAGATGA CCTCTGCTTA
GTATATGTTT ACATGCCTAA TGGTTCATTG CTAGACAGAC TCTCTTGCTT GGATGGTACT
CCACCACTTT CTTGGCACAT GAGATGCAAG ATTGCTCAGG GTGCAGCTAA TGGCATCAAT
TTTCTACATG AAAATCATCA TATTCATAGA GATATTAAAA GTGCAAATAT CTTACTGGAT
GAAGCTTTTA CTGCTAAAAT ATCTGACTTT GGCCTTGCAC GGGCTTCTGA GAAGTTTGCC
CAGACAGTCA TGACTAGCAG AATTGTGGGA ACAACAGCTT ATATGGCACC AGAAGCTTTG
CGTGGAGAAA TAACACCCAA ATCTGATATT TACAGCTTTG GTGTGGTTTT ACTAGAAATA
ATAACTGGAC TTCCAGCTGT GGATGAACAC CGTGAACCTC AGTTATTGCT AGATATTAAA
GAAGAAATTG AAGATGAAGA AAAGACAATT GAAGATTATA TTGATAAAAA GATGAATGAT
GCTGATTCCA CTTCAGTTGA AGCTATGTAC TCTGTTGCTA GTCAATGTCT GCATGAAAAG
AAAAATAAGA GACCAGACAT TAAGAAGGTT CAACAGCTGC TGCAAGAGAT GACAGCTTCT
TAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011536733.1
CDS181..1563
Misc Feature(1)205..498(+)
Misc Feature(2)214..414(+)
Misc Feature(3)253..474(+)
Misc Feature(4)688..1551(+)
Misc Feature(5)745..1542(+)
Misc Feature(6)754..1239(+)
Misc Feature(7)754..1167(+)
Misc Feature(8)766..1239(+)
Misc Feature(9)1162..1239(+)
Translation
Position Chain Variation Link
9 9 a, g dbSNP:199757889
9 9 -, g dbSNP:375801214
14 14 a, g dbSNP:571339542
27 27 -, aa dbSNP:60728702
28 28 -, g dbSNP:367869835
41 41 a, g dbSNP:371218069
58 58 g, t dbSNP:540648745
113 113 c, t dbSNP:188323164
126 126 g, t dbSNP:555695963
144 144 c, g dbSNP:4251432
185 185 a, g dbSNP:561048547
187 187 a, c dbSNP:752890848
191 191 c, t dbSNP:750912907
193 193 a, g dbSNP:56312115
196 196 a, c dbSNP:780540482
201 201 a, g dbSNP:201400143
207 207 a, c dbSNP:749572231
214 214 c, t dbSNP:377584435
215 215 a, g dbSNP:370524518
220 220 c, t dbSNP:748441973
224 224 a, g dbSNP:772274120
230 230 a, g dbSNP:778057572
234 234 a, g dbSNP:747100232
238 238 a, t dbSNP:143625818
243 243 a, g dbSNP:775601867
246 246 a, g dbSNP:762610309
256 256 a, g dbSNP:768548402
257 257 c, t dbSNP:138116867
262 262 a, c dbSNP:761596704
279 279 a, g dbSNP:115877973
292 292 a, c, g dbSNP:565544986
295 295 a, g dbSNP:113588409
297 297 a, t dbSNP:141039652
303 303 a, g dbSNP:551138584
311 311 g, t dbSNP:755265254
324 324 a, c dbSNP:779400593
329 329 a, t dbSNP:752850989
334 334 c, g dbSNP:758634981
342 342 a, g dbSNP:772625246
363 363 a, c dbSNP:778094168
365 365 c, g dbSNP:751881820
368 368 c, g dbSNP:572719034
386 386 a, c dbSNP:202134282
399 399 c, g, t dbSNP:374971450
403 403 a, g dbSNP:778743146
407 407 c, t dbSNP:200175187
410 410 a, c dbSNP:771900851
411 411 a, c dbSNP:772817746
424 424 a, g dbSNP:746729422
428 428 a, g dbSNP:770333429
432 432 c, t dbSNP:531518356
444 444 a, g dbSNP:776148970
446 446 c, t dbSNP:758974509
448 448 c, t dbSNP:765736285
453 453 c, t dbSNP:775885677
467 467 c, t dbSNP:763390934
470 470 c, t dbSNP:764597369
471 471 a, g dbSNP:17851028
474 474 g, t dbSNP:4251469
477 477 g, t dbSNP:757615268
483 483 c, t dbSNP:371634312
488 488 a, c, g dbSNP:774802371
494 494 -, tt dbSNP:763078267
498 498 c, g dbSNP:56338336
506 506 c, g dbSNP:750658320
513 513 -, a dbSNP:766515269
514 514 c, t dbSNP:756185480
523 523 a, g dbSNP:766433748
525 525 a, g dbSNP:752616965
526 526 a, g dbSNP:62642475
532 532 a, g dbSNP:540605517
551 551 a, c dbSNP:771105240
563 563 g, t dbSNP:777691189
569 569 a, g dbSNP:751316240
572 572 a, c dbSNP:757025762
579 579 a, g dbSNP:780877191
583 583 a, g dbSNP:745549223
586 586 a, g dbSNP:769345767
587 587 c, t dbSNP:779771826
594 594 a, g dbSNP:748825445
595 595 c, t dbSNP:769242383
603 603 c, t dbSNP:775016898
604 604 c, g dbSNP:762213099
605 605 a, t dbSNP:772359426
607 607 a, c dbSNP:773476797
611 611 c, g dbSNP:147079926
612 612 c, t dbSNP:766601065
617 617 g, t dbSNP:753847808
625 625 c, g dbSNP:375927287
626 626 a, g dbSNP:112228761
627 627 -, ctc dbSNP:556809469
633 633 -, a dbSNP:760054904
636 636 c, t dbSNP:763971241
642 642 a, t dbSNP:751549092
644 644 a, c dbSNP:757047226
647 647 a, g dbSNP:780919244
649 649 a, t dbSNP:750080879
650 650 g, t dbSNP:755668007
654 654 a, g dbSNP:779861617
656 656 a, t dbSNP:748754154
659 659 a, t dbSNP:201782959
664 664 a, g dbSNP:779528103
669 669 a, g, t dbSNP:748667787
685 685 c, t dbSNP:778367357
686 686 c, t dbSNP:747296110
702 702 g, t dbSNP:771324378
708 708 a, c dbSNP:776810085
709 709 a, g dbSNP:141209982
714 714 c, t dbSNP:769857009
717 717 c, g dbSNP:373731553
718 718 -, t dbSNP:753106997
727 727 c, t dbSNP:114951157
728 728 a, g dbSNP:186194251
741 741 c, t dbSNP:137949295
772 772 c, g dbSNP:773245379
772 772 -, g dbSNP:756802570
774 774 a, t dbSNP:113948349
778 778 c, g dbSNP:377074100
785 785 a, g dbSNP:191097365
792 792 c, t dbSNP:753538997
793 793 a, g dbSNP:370225662
818 818 a, t dbSNP:149453390
839 839 a, g dbSNP:781671136
848 848 c, t dbSNP:750733250
850 850 a, g dbSNP:201500711
858 858 a, g dbSNP:780121221
871 871 a, g dbSNP:111935971
877 877 a, g dbSNP:200082169
882 882 a, g, t dbSNP:778986995
885 885 a, t dbSNP:748253425
892 892 g, t dbSNP:770943951
900 900 c, t dbSNP:144854858
911 911 a, g dbSNP:781252640
922 922 c, t dbSNP:375755560
929 929 a, g dbSNP:769470855
938 938 c, g dbSNP:775286298
940 940 g, t dbSNP:762667477
941 941 a, t dbSNP:768303231
943 943 a, g dbSNP:774020314
946 946 a, g dbSNP:761277223
951 951 c, t dbSNP:767075679
965 965 a, g dbSNP:773708174
966 966 c, t dbSNP:201704200
967 967 a, g dbSNP:112517169
971 971 a, t dbSNP:766812248
987 987 a, c dbSNP:754184412
999 999 a, t dbSNP:370042548
1002 1002 c, g, t dbSNP:755330743
1005 1005 c, t dbSNP:140210540
1012 1012 a, g dbSNP:757311009
1013 1013 a, g, t dbSNP:780005506
1014 1014 c, t dbSNP:754767022
1030 1030 c, t dbSNP:778616067
1035 1035 g, t dbSNP:747953616
1040 1040 g, t dbSNP:771667566
1045 1045 c, t dbSNP:373167326
1050 1050 c, g dbSNP:145622820
1054 1054 c, g dbSNP:770396929
1057 1057 c, t dbSNP:121908002
1058 1058 a, g dbSNP:760186666
1060 1060 a, g dbSNP:200883675
1061 1061 a, g dbSNP:775937250
1069 1069 a, g dbSNP:556565376
1073 1073 a, g, t dbSNP:568782766
1079 1079 a, g dbSNP:376948391
1080 1080 c, t dbSNP:536101269
1086 1086 a, t dbSNP:750525615
1096 1096 a, c dbSNP:115573494
1097 1097 a, t dbSNP:778846957
1103 1103 a, t dbSNP:752451445
1104 1104 a, t dbSNP:374532899
1115 1115 c, t dbSNP:777460833
1123 1123 a, g dbSNP:751373844
1130 1130 a, t dbSNP:756802579
1141 1141 a, g dbSNP:780917972
1142 1142 a, t dbSNP:147301051
1145 1145 c, g dbSNP:769465559
1153 1153 a, g dbSNP:780681951
1170 1170 c, t dbSNP:749864140
1172 1172 g, t dbSNP:769214804
1180 1180 c, t dbSNP:774525787
1181 1181 a, g dbSNP:748570560
1198 1198 g, t dbSNP:772148472
1209 1209 c, t dbSNP:773464920
1217 1217 a, g dbSNP:368108618
1237 1237 a, g dbSNP:140869783
1243 1243 a, c, g dbSNP:142376871
1253 1253 a, t dbSNP:775402267
1261 1261 c, t dbSNP:762731086
1262 1262 a, g dbSNP:764106350
1264 1264 a, g dbSNP:751261930
1278 1278 c, t dbSNP:757104944
1289 1289 c, t dbSNP:372448559
1291 1291 g, t dbSNP:750146218
1300 1300 a, g dbSNP:779468411
1303 1303 a, g dbSNP:755668302
1304 1304 c, t dbSNP:779773825
1322 1322 c, t dbSNP:767476422
1325 1325 c, g dbSNP:750092963
1326 1326 -, tg dbSNP:764404378
1328 1328 a, g, t dbSNP:755772831
1329 1329 a, g dbSNP:753251856
1341 1341 a, g dbSNP:757918302
1343 1343 a, t dbSNP:151272504
1349 1349 a, g dbSNP:4251583
1351 1351 c, t dbSNP:114820168
1352 1352 a, g dbSNP:55944915
1357 1357 c, g dbSNP:758688655
1371 1371 a, g dbSNP:773095217
1379 1379 -, aag dbSNP:758148548
1381 1381 a, g dbSNP:760580944
1384 1384 g, t dbSNP:766102187
1402 1402 a, c dbSNP:547810966
1405 1405 a, g dbSNP:758956283
1408 1408 a, g dbSNP:764868568
1409 1409 c, t dbSNP:146538989
1420 1420 a, g dbSNP:758846627
1421 1421 c, t dbSNP:778274322
1441 1441 a, g dbSNP:751879000
1459 1459 a, g dbSNP:757657887
1462 1462 a, g dbSNP:4251545
1466 1466 c, t dbSNP:533369384
1471 1471 g, t dbSNP:769754150
1473 1473 g, t dbSNP:780112532
1474 1474 c, g dbSNP:749338213
1477 1477 a, g dbSNP:772098333
1478 1478 c, g dbSNP:773326632
1481 1481 c, g dbSNP:760527974
1482 1482 c, t dbSNP:551524673
1486 1486 a, t dbSNP:770908857
1487 1487 c, g dbSNP:776143317
1491 1491 a, g dbSNP:759164006
1494 1494 c, t dbSNP:144842940
1509 1509 c, g dbSNP:752201648
1511 1511 g, t dbSNP:780633823
1513 1513 a, c, t dbSNP:762479548
1514 1514 a, c dbSNP:752108446
1518 1518 c, t dbSNP:138719119
1520 1520 a, g, t dbSNP:569421834
1524 1524 g, t dbSNP:756298129
1531 1531 c, g dbSNP:762236455
1545 1545 a, g dbSNP:767921421
1546 1546 a, g dbSNP:148873968
1547 1547 a, t dbSNP:372068514
1551 1551 a, g dbSNP:766606181
1553 1553 c, t dbSNP:754175088
1567 1567 c, t dbSNP:755104443
1570 1570 a, g dbSNP:567188868
1580 1580 a, t dbSNP:748253330
1582 1582 c, t dbSNP:199727839
1587 1587 a, g dbSNP:781021520
1590 1590 c, t dbSNP:781731425
1596 1596 a, g dbSNP:745643347
1604 1604 a, g dbSNP:553330681
1606 1606 c, g dbSNP:769526114
1614 1614 -, t dbSNP:755909125
1615 1615 a, t dbSNP:184380424
1675 1675 a, g dbSNP:577974384
1709 1709 c, t dbSNP:4251546
1713 1713 a, g dbSNP:182322900
1808 1808 a, g dbSNP:187201106
1833 1833 c, t dbSNP:4251547
1844 1844 a, g dbSNP:4251548
1868 1868 g, t dbSNP:115845154
1882 1882 a, g dbSNP:769535835
1905 1905 g, t dbSNP:147004395
1906 1906 a, g dbSNP:770150730
1960 1960 a, g dbSNP:773095431
1973 1973 a, g dbSNP:559634238
1991 1991 a, t dbSNP:4251549
2025 2025 c, t dbSNP:191617315
2051 2051 a, t dbSNP:183559035
2083 2083 a, t dbSNP:4251550
2085 2085 a, c dbSNP:548838962
2171 2171 a, g dbSNP:4251551
2183 2183 a, g dbSNP:138178820
2186 2186 a, g dbSNP:4251552
2201 2201 a, c dbSNP:571568921
2229 2229 g, t dbSNP:774327325
2233 2233 g, t dbSNP:759513055
2244 2244 a, g dbSNP:538982204
2331 2331 c, t dbSNP:576622168
2381 2381 a, t dbSNP:767298091
2403 2403 a, c dbSNP:752547785
2412 2412 -, a dbSNP:36085436
2417 2417 a, g dbSNP:567630494
2439 2439 a, g dbSNP:566365168
2519 2519 a, g dbSNP:575385169
2530 2530 a, g dbSNP:542787354
2552 2552 g, t dbSNP:4251584
2630 2630 a, g dbSNP:574043929
2639 2639 c, t dbSNP:541034481
2659 2659 a, c dbSNP:559273571
2665 2665 a, g dbSNP:753237737
2670 2670 a, c dbSNP:777619096
2671 2671 a, g dbSNP:141847763
2724 2724 a, g dbSNP:545334973
2733 2733 c, t dbSNP:563991845
2750 2750 c, t dbSNP:755598323
2761 2761 a, c dbSNP:531038940
2774 2774 a, g dbSNP:781639576
2835 2835 g, t dbSNP:182497864
2836 2836 c, t dbSNP:111571285
2852 2852 a, c dbSNP:150604439
2859 2859 c, t dbSNP:528147840
2865 2865 a, c dbSNP:554628455
2869 2869 c, t dbSNP:770097373
2888 2888 a, g dbSNP:139778399
2892 2892 a, g dbSNP:563645022
2902 2902 -, ataa dbSNP:138875923
2903 2903 -, ataa dbSNP:527750692
2906 2906 -, ataa dbSNP:531640584
2921 2921 -, aaat dbSNP:368676868
2927 2927 g, t dbSNP:186825265
2949 2949 a, g dbSNP:530704544
2964 2964 g, t dbSNP:4251553
3078 3078 c, t dbSNP:536073958
3087 3087 c, t dbSNP:554707596
3088 3088 a, g dbSNP:542838156
3097 3097 a, g dbSNP:574051595
3106 3106 a, c, g dbSNP:139675032
3151 3151 a, g dbSNP:778083287
3170 3170 a, t dbSNP:553037446
3231 3231 c, g dbSNP:576135948
3245 3245 g, t dbSNP:192534409
3251 3251 -, g dbSNP:778897449
3255 3255 c, t dbSNP:544979835
3259 3259 a, g dbSNP:543426658
3282 3282 a, g dbSNP:771014920
3316 3316 c, t dbSNP:558662153
3323 3323 a, g dbSNP:144535012
3340 3340 a, t dbSNP:4251554
3357 3357 c, t dbSNP:4251555
3387 3387 -, ctt dbSNP:79794889
3388 3388 -, t dbSNP:538947924
3439 3439 a, g dbSNP:528687727
3446 3446 a, g dbSNP:546754318
3450 3450 c, t dbSNP:4251556
3451 3451 a, g dbSNP:183931852
3456 3456 c, t dbSNP:4251557
3458 3458 a, g dbSNP:4251558
3496 3496 a, c dbSNP:536497843
3504 3504 a, c dbSNP:548182708
3537 3537 a, g dbSNP:12321678
3577 3577 a, g dbSNP:533723416
3603 3603 a, g dbSNP:371821781
3624 3624 c, g dbSNP:533412197
3634 3634 c, t dbSNP:776314485
3643 3643 c, t dbSNP:776764561
3646 3646 c, g dbSNP:111813306
3663 3663 a, g dbSNP:534645871
3686 3686 g, t dbSNP:374721629
3751 3751 a, g dbSNP:1141168
3761 3761 c, t dbSNP:563217241
3786 3786 g, t dbSNP:769976979
3818 3818 g, t dbSNP:557276770
3829 3829 a, g dbSNP:4251560
3864 3864 g, t dbSNP:543346753
3908 3908 a, g dbSNP:368152755
3912 3912 c, t dbSNP:749964168
3921 3921 g, t dbSNP:554949427
3970 3970 -, tgtttaga dbSNP:747864158
3996 3996 c, t dbSNP:9849
4107 4107 a, g dbSNP:76405665
4125 4125 c, g, t dbSNP:145856072
4126 4126 a, g dbSNP:756483703
4128 4128 a, g dbSNP:549897461
4140 4140 g, t dbSNP:549623720
4175 4175 c, t dbSNP:147831464
4228 4228 c, t dbSNP:532207331
4229 4229 a, g, t dbSNP:375914722
4232 4232 c, t dbSNP:12310328
4265 4265 a, g dbSNP:749524568
4267 4267 c, t dbSNP:4251562
4288 4288 c, g dbSNP:766712571
4315 4315 c, t dbSNP:369823166
4316 4316 a, g dbSNP:562549392
4324 4324 a, g dbSNP:4251563
4367 4367 c, t dbSNP:771957948
4380 4380 a, g dbSNP:4251564

Target ORF information:

RefSeq Version XM_011538431
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011538431

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAACAAAC CCATAACACC ATCAACATAT GTGCGCTGCC TCAATGTTGG ACTAATTAGG 
AAGCTGTCAG ATTTTATTGA TCCTCAAGAA GGATGGAAGA AGTTAGCTGT AGCTATTAAA
AAACCATCTG GTGATGATAG ATACAATCAG TTTCACATAA GGAGATTTGA AGCATTACTT
CAAACTGGAA AAAGTCCCAC TTCTGAATTA CTGTTTGACT GGGGCACCAC AAATTGCACA
GTTGGTGATC TTGTGGATCT TTTGATCCAA AATGAATTTT TTGCTCCTGC GAGTCTTTTG
CTCCCAGATG CTGTTCCCAA AACTGCTAAT ACACTACCTT CTAAAGAAGC TATAACAGTT
CAGCAAAAAC AGATGCCTTT CTGTGACAAA GACAGGACAT TGATGACACC TGTGCAGAAT
CTTGAACAAA GCTATATGCC ACCTGACTCC TCAAGTCCAG AAAATAAAAG TTTAGAAGTT
AGTGATACAC GTTTTCACAG TTTTTCATTT TATGAATTGA AGAATGTCAC AAATAACTTT
GATGAACGAC CCATTTCTGT TGGTGGTAAT AAAATGGGAG AGGGAGGATT TGGAGTTGTA
TATAAAGGCT ACGTAAATAA CACAACTGTG GCAGTGAAGA AGCTTGCAGC AATGGTTGAC
ATTACTACTG AAGAACTGAA ACAGCAGTTT GATCAAGAAA TAAAAGTAAT GGCAAAGTGT
CAACATGAAA ACTTAGTAGA ACTACTTGGT TTCTCAAGTG ATGGAGATGA CCTCTGCTTA
GTATATGTTT ACATGCCTAA TGGTTCATTG CTAGACAGAC TCTCTTGCTT GGATGGTACT
CCACCACTTT CTTGGCACAT GAGATGCAAG ATTGCTCAGG GTGCAGCTAA TGGCATCAAT
TTTCTACATG AAAATCATCA TATTCATAGA GATATTAAAA GTGCAAATAT CTTACTGGAT
GAAGCTTTTA CTGCTAAAAT ATCTGACTTT GGCCTTGCAC GGGCTTCTGA GAAGTTTGCC
CAGACAGTCA TGACTAGCAG AATTGTGGGA ACAACAGCTT ATATGGCACC AGAAGCTTTG
CGTGGAGAAA TAACACCCAA ATCTGATATT TACAGCTTTG GTGTGGTTTT ACTAGAAATA
ATAACTGGAC TTCCAGCTGT GGATGAACAC CGTGAACCTC AGTTATTGCT AGATATTAAA
GAAGAAATTG AAGATGAAGA AAAGACAATT GAAGATTATA TTGATAAAAA GATGAATGAT
GCTGATTCCA CTTCAGTTGA AGCTATGTAC TCTGTTGCTA GTCAATGTCT GCATGAAAAG
AAAAATAAGA GACCAGACAT TAAGAAGGTT CAACAGCTGC TGCAAGAGAT GACAGCTTCT
TAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu15137D
Sequence Information ORF Nucleotide Sequence (Length: 1383bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product interleukin-1 receptor-associated kinase 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578823831. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAACAAAC CCATAACACC ATCAACATAT GTGCGCTGCC TCAATGTTGG ACTAATTAGG 
AAGCTGTCAG ATTTTATTGA TCCTCAAGAA GGATGGAAGA AGTTAGCTGT AGCTATTAAA
AAACCATCTG GTGATGATAG ATACAATCAG TTTCACATAA GGAGATTTGA AGCATTACTT
CAAACTGGAA AAAGTCCCAC TTCTGAATTA CTGTTTGACT GGGGCACCAC AAATTGCACA
GTTGGTGATC TTGTGGATCT TTTGATCCAA AATGAATTTT TTGCTCCTGC GAGTCTTTTG
CTCCCAGATG CTGTTCCCAA AACTGCTAAT ACACTACCTT CTAAAGAAGC TATAACAGTT
CAGCAAAAAC AGATGCCTTT CTGTGACAAA GACAGGACAT TGATGACACC TGTGCAGAAT
CTTGAACAAA GCTATATGCC ACCTGACTCC TCAAGTCCAG AAAATAAAAG TTTAGAAGTT
AGTGATACAC GTTTTCACAG TTTTTCATTT TATGAATTGA AGAATGTCAC AAATAACTTT
GATGAACGAC CCATTTCTGT TGGTGGTAAT AAAATGGGAG AGGGAGGATT TGGAGTTGTA
TATAAAGGCT ACGTAAATAA CACAACTGTG GCAGTGAAGA AGCTTGCAGC AATGGTTGAC
ATTACTACTG AAGAACTGAA ACAGCAGTTT GATCAAGAAA TAAAAGTAAT GGCAAAGTGT
CAACATGAAA ACTTAGTAGA ACTACTTGGT TTCTCAAGTG ATGGAGATGA CCTCTGCTTA
GTATATGTTT ACATGCCTAA TGGTTCATTG CTAGACAGAC TCTCTTGCTT GGATGGTACT
CCACCACTTT CTTGGCACAT GAGATGCAAG ATTGCTCAGG GTGCAGCTAA TGGCATCAAT
TTTCTACATG AAAATCATCA TATTCATAGA GATATTAAAA GTGCAAATAT CTTACTGGAT
GAAGCTTTTA CTGCTAAAAT ATCTGACTTT GGCCTTGCAC GGGCTTCTGA GAAGTTTGCC
CAGACAGTCA TGACTAGCAG AATTGTGGGA ACAACAGCTT ATATGGCACC AGAAGCTTTG
CGTGGAGAAA TAACACCCAA ATCTGATATT TACAGCTTTG GTGTGGTTTT ACTAGAAATA
ATAACTGGAC TTCCAGCTGT GGATGAACAC CGTGAACCTC AGTTATTGCT AGATATTAAA
GAAGAAATTG AAGATGAAGA AAAGACAATT GAAGATTATA TTGATAAAAA GATGAATGAT
GCTGATTCCA CTTCAGTTGA AGCTATGTAC TCTGTTGCTA GTCAATGTCT GCATGAAAAG
AAAAATAAGA GACCAGACAT TAAGAAGGTT CAACAGCTGC TGCAAGAGAT GACAGCTTCT
TAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_006719501.1
CDS443..1825
Misc Feature(1)467..760(+)
Misc Feature(2)476..676(+)
Misc Feature(3)515..736(+)
Misc Feature(4)950..1813(+)
Misc Feature(5)1007..1804(+)
Misc Feature(6)1016..1501(+)
Misc Feature(7)1016..1429(+)
Misc Feature(8)1028..1501(+)
Misc Feature(9)1424..1501(+)
Translation
Position Chain Variation Link
8 8 a, g dbSNP:546604862
20 20 a, g dbSNP:750677998
23 23 c, g dbSNP:763261637
30 30 a, t dbSNP:758663016
101 101 c, t dbSNP:571580877
103 103 a, c dbSNP:538974475
118 118 c, t dbSNP:751628808
163 163 a, g dbSNP:557115716
191 191 c, t dbSNP:4251567
224 224 a, c dbSNP:576881180
228 228 g, t dbSNP:554603533
241 241 c, t dbSNP:4251423
264 264 a, g dbSNP:572864111
332 332 c, t dbSNP:4251568
339 339 c, t dbSNP:547329673
372 372 a, c dbSNP:181596834
388 388 g, t dbSNP:555695963
406 406 c, g dbSNP:4251432
447 447 a, g dbSNP:561048547
449 449 a, c dbSNP:752890848
453 453 c, t dbSNP:750912907
455 455 a, g dbSNP:56312115
458 458 a, c dbSNP:780540482
463 463 a, g dbSNP:201400143
469 469 a, c dbSNP:749572231
476 476 c, t dbSNP:377584435
477 477 a, g dbSNP:370524518
482 482 c, t dbSNP:748441973
486 486 a, g dbSNP:772274120
492 492 a, g dbSNP:778057572
496 496 a, g dbSNP:747100232
500 500 a, t dbSNP:143625818
505 505 a, g dbSNP:775601867
508 508 a, g dbSNP:762610309
518 518 a, g dbSNP:768548402
519 519 c, t dbSNP:138116867
524 524 a, c dbSNP:761596704
541 541 a, g dbSNP:115877973
554 554 a, c, g dbSNP:565544986
557 557 a, g dbSNP:113588409
559 559 a, t dbSNP:141039652
565 565 a, g dbSNP:551138584
573 573 g, t dbSNP:755265254
586 586 a, c dbSNP:779400593
591 591 a, t dbSNP:752850989
596 596 c, g dbSNP:758634981
604 604 a, g dbSNP:772625246
625 625 a, c dbSNP:778094168
627 627 c, g dbSNP:751881820
630 630 c, g dbSNP:572719034
648 648 a, c dbSNP:202134282
661 661 c, g, t dbSNP:374971450
665 665 a, g dbSNP:778743146
669 669 c, t dbSNP:200175187
672 672 a, c dbSNP:771900851
673 673 a, c dbSNP:772817746
686 686 a, g dbSNP:746729422
690 690 a, g dbSNP:770333429
694 694 c, t dbSNP:531518356
706 706 a, g dbSNP:776148970
708 708 c, t dbSNP:758974509
710 710 c, t dbSNP:765736285
715 715 c, t dbSNP:775885677
729 729 c, t dbSNP:763390934
732 732 c, t dbSNP:764597369
733 733 a, g dbSNP:17851028
736 736 g, t dbSNP:4251469
739 739 g, t dbSNP:757615268
745 745 c, t dbSNP:371634312
750 750 a, c, g dbSNP:774802371
756 756 -, tt dbSNP:763078267
760 760 c, g dbSNP:56338336
768 768 c, g dbSNP:750658320
775 775 -, a dbSNP:766515269
776 776 c, t dbSNP:756185480
785 785 a, g dbSNP:766433748
787 787 a, g dbSNP:752616965
788 788 a, g dbSNP:62642475
794 794 a, g dbSNP:540605517
813 813 a, c dbSNP:771105240
825 825 g, t dbSNP:777691189
831 831 a, g dbSNP:751316240
834 834 a, c dbSNP:757025762
841 841 a, g dbSNP:780877191
845 845 a, g dbSNP:745549223
848 848 a, g dbSNP:769345767
849 849 c, t dbSNP:779771826
856 856 a, g dbSNP:748825445
857 857 c, t dbSNP:769242383
865 865 c, t dbSNP:775016898
866 866 c, g dbSNP:762213099
867 867 a, t dbSNP:772359426
869 869 a, c dbSNP:773476797
873 873 c, g dbSNP:147079926
874 874 c, t dbSNP:766601065
879 879 g, t dbSNP:753847808
887 887 c, g dbSNP:375927287
888 888 a, g dbSNP:112228761
889 889 -, ctc dbSNP:556809469
895 895 -, a dbSNP:760054904
898 898 c, t dbSNP:763971241
904 904 a, t dbSNP:751549092
906 906 a, c dbSNP:757047226
909 909 a, g dbSNP:780919244
911 911 a, t dbSNP:750080879
912 912 g, t dbSNP:755668007
916 916 a, g dbSNP:779861617
918 918 a, t dbSNP:748754154
921 921 a, t dbSNP:201782959
926 926 a, g dbSNP:779528103
931 931 a, g, t dbSNP:748667787
947 947 c, t dbSNP:778367357
948 948 c, t dbSNP:747296110
964 964 g, t dbSNP:771324378
970 970 a, c dbSNP:776810085
971 971 a, g dbSNP:141209982
976 976 c, t dbSNP:769857009
979 979 c, g dbSNP:373731553
980 980 -, t dbSNP:753106997
989 989 c, t dbSNP:114951157
990 990 a, g dbSNP:186194251
1003 1003 c, t dbSNP:137949295
1034 1034 c, g dbSNP:773245379
1034 1034 -, g dbSNP:756802570
1036 1036 a, t dbSNP:113948349
1040 1040 c, g dbSNP:377074100
1047 1047 a, g dbSNP:191097365
1054 1054 c, t dbSNP:753538997
1055 1055 a, g dbSNP:370225662
1080 1080 a, t dbSNP:149453390
1101 1101 a, g dbSNP:781671136
1110 1110 c, t dbSNP:750733250
1112 1112 a, g dbSNP:201500711
1120 1120 a, g dbSNP:780121221
1133 1133 a, g dbSNP:111935971
1139 1139 a, g dbSNP:200082169
1144 1144 a, g, t dbSNP:778986995
1147 1147 a, t dbSNP:748253425
1154 1154 g, t dbSNP:770943951
1162 1162 c, t dbSNP:144854858
1173 1173 a, g dbSNP:781252640
1184 1184 c, t dbSNP:375755560
1191 1191 a, g dbSNP:769470855
1200 1200 c, g dbSNP:775286298
1202 1202 g, t dbSNP:762667477
1203 1203 a, t dbSNP:768303231
1205 1205 a, g dbSNP:774020314
1208 1208 a, g dbSNP:761277223
1213 1213 c, t dbSNP:767075679
1227 1227 a, g dbSNP:773708174
1228 1228 c, t dbSNP:201704200
1229 1229 a, g dbSNP:112517169
1233 1233 a, t dbSNP:766812248
1249 1249 a, c dbSNP:754184412
1261 1261 a, t dbSNP:370042548
1264 1264 c, g, t dbSNP:755330743
1267 1267 c, t dbSNP:140210540
1274 1274 a, g dbSNP:757311009
1275 1275 a, g, t dbSNP:780005506
1276 1276 c, t dbSNP:754767022
1292 1292 c, t dbSNP:778616067
1297 1297 g, t dbSNP:747953616
1302 1302 g, t dbSNP:771667566
1307 1307 c, t dbSNP:373167326
1312 1312 c, g dbSNP:145622820
1316 1316 c, g dbSNP:770396929
1319 1319 c, t dbSNP:121908002
1320 1320 a, g dbSNP:760186666
1322 1322 a, g dbSNP:200883675
1323 1323 a, g dbSNP:775937250
1331 1331 a, g dbSNP:556565376
1335 1335 a, g, t dbSNP:568782766
1341 1341 a, g dbSNP:376948391
1342 1342 c, t dbSNP:536101269
1348 1348 a, t dbSNP:750525615
1358 1358 a, c dbSNP:115573494
1359 1359 a, t dbSNP:778846957
1365 1365 a, t dbSNP:752451445
1366 1366 a, t dbSNP:374532899
1377 1377 c, t dbSNP:777460833
1385 1385 a, g dbSNP:751373844
1392 1392 a, t dbSNP:756802579
1403 1403 a, g dbSNP:780917972
1404 1404 a, t dbSNP:147301051
1407 1407 c, g dbSNP:769465559
1415 1415 a, g dbSNP:780681951
1432 1432 c, t dbSNP:749864140
1434 1434 g, t dbSNP:769214804
1442 1442 c, t dbSNP:774525787
1443 1443 a, g dbSNP:748570560
1460 1460 g, t dbSNP:772148472
1471 1471 c, t dbSNP:773464920
1479 1479 a, g dbSNP:368108618
1499 1499 a, g dbSNP:140869783
1505 1505 a, c, g dbSNP:142376871
1515 1515 a, t dbSNP:775402267
1523 1523 c, t dbSNP:762731086
1524 1524 a, g dbSNP:764106350
1526 1526 a, g dbSNP:751261930
1540 1540 c, t dbSNP:757104944
1551 1551 c, t dbSNP:372448559
1553 1553 g, t dbSNP:750146218
1562 1562 a, g dbSNP:779468411
1565 1565 a, g dbSNP:755668302
1566 1566 c, t dbSNP:779773825
1584 1584 c, t dbSNP:767476422
1587 1587 c, g dbSNP:750092963
1588 1588 -, tg dbSNP:764404378
1590 1590 a, g, t dbSNP:755772831
1591 1591 a, g dbSNP:753251856
1603 1603 a, g dbSNP:757918302
1605 1605 a, t dbSNP:151272504
1611 1611 a, g dbSNP:4251583
1613 1613 c, t dbSNP:114820168
1614 1614 a, g dbSNP:55944915
1619 1619 c, g dbSNP:758688655
1633 1633 a, g dbSNP:773095217
1641 1641 -, aag dbSNP:758148548
1643 1643 a, g dbSNP:760580944
1646 1646 g, t dbSNP:766102187
1664 1664 a, c dbSNP:547810966
1667 1667 a, g dbSNP:758956283
1670 1670 a, g dbSNP:764868568
1671 1671 c, t dbSNP:146538989
1682 1682 a, g dbSNP:758846627
1683 1683 c, t dbSNP:778274322
1703 1703 a, g dbSNP:751879000
1721 1721 a, g dbSNP:757657887
1724 1724 a, g dbSNP:4251545
1728 1728 c, t dbSNP:533369384
1733 1733 g, t dbSNP:769754150
1735 1735 g, t dbSNP:780112532
1736 1736 c, g dbSNP:749338213
1739 1739 a, g dbSNP:772098333
1740 1740 c, g dbSNP:773326632
1743 1743 c, g dbSNP:760527974
1744 1744 c, t dbSNP:551524673
1748 1748 a, t dbSNP:770908857
1749 1749 c, g dbSNP:776143317
1753 1753 a, g dbSNP:759164006
1756 1756 c, t dbSNP:144842940
1771 1771 c, g dbSNP:752201648
1773 1773 g, t dbSNP:780633823
1775 1775 a, c, t dbSNP:762479548
1776 1776 a, c dbSNP:752108446
1780 1780 c, t dbSNP:138719119
1782 1782 a, g, t dbSNP:569421834
1786 1786 g, t dbSNP:756298129
1793 1793 c, g dbSNP:762236455
1807 1807 a, g dbSNP:767921421
1808 1808 a, g dbSNP:148873968
1809 1809 a, t dbSNP:372068514
1813 1813 a, g dbSNP:766606181
1815 1815 c, t dbSNP:754175088
1829 1829 c, t dbSNP:755104443
1832 1832 a, g dbSNP:567188868
1842 1842 a, t dbSNP:748253330
1844 1844 c, t dbSNP:199727839
1849 1849 a, g dbSNP:781021520
1852 1852 c, t dbSNP:781731425
1858 1858 a, g dbSNP:745643347
1866 1866 a, g dbSNP:553330681
1868 1868 c, g dbSNP:769526114
1876 1876 -, t dbSNP:755909125
1877 1877 a, t dbSNP:184380424
1937 1937 a, g dbSNP:577974384
1971 1971 c, t dbSNP:4251546
1975 1975 a, g dbSNP:182322900
2070 2070 a, g dbSNP:187201106
2095 2095 c, t dbSNP:4251547
2106 2106 a, g dbSNP:4251548
2130 2130 g, t dbSNP:115845154
2144 2144 a, g dbSNP:769535835
2167 2167 g, t dbSNP:147004395
2168 2168 a, g dbSNP:770150730
2222 2222 a, g dbSNP:773095431
2235 2235 a, g dbSNP:559634238
2253 2253 a, t dbSNP:4251549
2287 2287 c, t dbSNP:191617315
2313 2313 a, t dbSNP:183559035
2345 2345 a, t dbSNP:4251550
2347 2347 a, c dbSNP:548838962
2433 2433 a, g dbSNP:4251551
2445 2445 a, g dbSNP:138178820
2448 2448 a, g dbSNP:4251552
2463 2463 a, c dbSNP:571568921
2491 2491 g, t dbSNP:774327325
2495 2495 g, t dbSNP:759513055
2506 2506 a, g dbSNP:538982204
2593 2593 c, t dbSNP:576622168
2643 2643 a, t dbSNP:767298091
2665 2665 a, c dbSNP:752547785
2674 2674 -, a dbSNP:36085436
2679 2679 a, g dbSNP:567630494
2701 2701 a, g dbSNP:566365168
2781 2781 a, g dbSNP:575385169
2792 2792 a, g dbSNP:542787354
2814 2814 g, t dbSNP:4251584
2892 2892 a, g dbSNP:574043929
2901 2901 c, t dbSNP:541034481
2921 2921 a, c dbSNP:559273571
2927 2927 a, g dbSNP:753237737
2932 2932 a, c dbSNP:777619096
2933 2933 a, g dbSNP:141847763
2986 2986 a, g dbSNP:545334973
2995 2995 c, t dbSNP:563991845
3012 3012 c, t dbSNP:755598323
3023 3023 a, c dbSNP:531038940
3036 3036 a, g dbSNP:781639576
3097 3097 g, t dbSNP:182497864
3098 3098 c, t dbSNP:111571285
3114 3114 a, c dbSNP:150604439
3121 3121 c, t dbSNP:528147840
3127 3127 a, c dbSNP:554628455
3131 3131 c, t dbSNP:770097373
3150 3150 a, g dbSNP:139778399
3154 3154 a, g dbSNP:563645022
3164 3164 -, ataa dbSNP:138875923
3165 3165 -, ataa dbSNP:527750692
3168 3168 -, ataa dbSNP:531640584
3183 3183 -, aaat dbSNP:368676868
3189 3189 g, t dbSNP:186825265
3211 3211 a, g dbSNP:530704544
3226 3226 g, t dbSNP:4251553
3340 3340 c, t dbSNP:536073958
3349 3349 c, t dbSNP:554707596
3350 3350 a, g dbSNP:542838156
3359 3359 a, g dbSNP:574051595
3368 3368 a, c, g dbSNP:139675032
3413 3413 a, g dbSNP:778083287
3432 3432 a, t dbSNP:553037446
3493 3493 c, g dbSNP:576135948
3507 3507 g, t dbSNP:192534409
3513 3513 -, g dbSNP:778897449
3517 3517 c, t dbSNP:544979835
3521 3521 a, g dbSNP:543426658
3544 3544 a, g dbSNP:771014920
3578 3578 c, t dbSNP:558662153
3585 3585 a, g dbSNP:144535012
3602 3602 a, t dbSNP:4251554
3619 3619 c, t dbSNP:4251555
3649 3649 -, ctt dbSNP:79794889
3650 3650 -, t dbSNP:538947924
3701 3701 a, g dbSNP:528687727
3708 3708 a, g dbSNP:546754318
3712 3712 c, t dbSNP:4251556
3713 3713 a, g dbSNP:183931852
3718 3718 c, t dbSNP:4251557
3720 3720 a, g dbSNP:4251558
3758 3758 a, c dbSNP:536497843
3766 3766 a, c dbSNP:548182708
3799 3799 a, g dbSNP:12321678
3839 3839 a, g dbSNP:533723416
3865 3865 a, g dbSNP:371821781
3886 3886 c, g dbSNP:533412197
3896 3896 c, t dbSNP:776314485
3905 3905 c, t dbSNP:776764561
3908 3908 c, g dbSNP:111813306
3925 3925 a, g dbSNP:534645871
3948 3948 g, t dbSNP:374721629
4013 4013 a, g dbSNP:1141168
4023 4023 c, t dbSNP:563217241
4048 4048 g, t dbSNP:769976979
4080 4080 g, t dbSNP:557276770
4091 4091 a, g dbSNP:4251560
4126 4126 g, t dbSNP:543346753
4170 4170 a, g dbSNP:368152755
4174 4174 c, t dbSNP:749964168
4183 4183 g, t dbSNP:554949427
4232 4232 -, tgtttaga dbSNP:747864158
4258 4258 c, t dbSNP:9849
4369 4369 a, g dbSNP:76405665
4387 4387 c, g, t dbSNP:145856072
4388 4388 a, g dbSNP:756483703
4390 4390 a, g dbSNP:549897461
4402 4402 g, t dbSNP:549623720
4437 4437 c, t dbSNP:147831464
4490 4490 c, t dbSNP:532207331
4491 4491 a, g, t dbSNP:375914722
4494 4494 c, t dbSNP:12310328
4527 4527 a, g dbSNP:749524568
4529 4529 c, t dbSNP:4251562
4550 4550 c, g dbSNP:766712571
4577 4577 c, t dbSNP:369823166
4578 4578 a, g dbSNP:562549392
4586 4586 a, g dbSNP:4251563
4629 4629 c, t dbSNP:771957948
4642 4642 a, g dbSNP:4251564

Target ORF information:

RefSeq Version XM_006719438
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_006719438

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAACAAAC CCATAACACC ATCAACATAT GTGCGCTGCC TCAATGTTGG ACTAATTAGG 
AAGCTGTCAG ATTTTATTGA TCCTCAAGAA GGATGGAAGA AGTTAGCTGT AGCTATTAAA
AAACCATCTG GTGATGATAG ATACAATCAG TTTCACATAA GGAGATTTGA AGCATTACTT
CAAACTGGAA AAAGTCCCAC TTCTGAATTA CTGTTTGACT GGGGCACCAC AAATTGCACA
GTTGGTGATC TTGTGGATCT TTTGATCCAA AATGAATTTT TTGCTCCTGC GAGTCTTTTG
CTCCCAGATG CTGTTCCCAA AACTGCTAAT ACACTACCTT CTAAAGAAGC TATAACAGTT
CAGCAAAAAC AGATGCCTTT CTGTGACAAA GACAGGACAT TGATGACACC TGTGCAGAAT
CTTGAACAAA GCTATATGCC ACCTGACTCC TCAAGTCCAG AAAATAAAAG TTTAGAAGTT
AGTGATACAC GTTTTCACAG TTTTTCATTT TATGAATTGA AGAATGTCAC AAATAACTTT
GATGAACGAC CCATTTCTGT TGGTGGTAAT AAAATGGGAG AGGGAGGATT TGGAGTTGTA
TATAAAGGCT ACGTAAATAA CACAACTGTG GCAGTGAAGA AGCTTGCAGC AATGGTTGAC
ATTACTACTG AAGAACTGAA ACAGCAGTTT GATCAAGAAA TAAAAGTAAT GGCAAAGTGT
CAACATGAAA ACTTAGTAGA ACTACTTGGT TTCTCAAGTG ATGGAGATGA CCTCTGCTTA
GTATATGTTT ACATGCCTAA TGGTTCATTG CTAGACAGAC TCTCTTGCTT GGATGGTACT
CCACCACTTT CTTGGCACAT GAGATGCAAG ATTGCTCAGG GTGCAGCTAA TGGCATCAAT
TTTCTACATG AAAATCATCA TATTCATAGA GATATTAAAA GTGCAAATAT CTTACTGGAT
GAAGCTTTTA CTGCTAAAAT ATCTGACTTT GGCCTTGCAC GGGCTTCTGA GAAGTTTGCC
CAGACAGTCA TGACTAGCAG AATTGTGGGA ACAACAGCTT ATATGGCACC AGAAGCTTTG
CGTGGAGAAA TAACACCCAA ATCTGATATT TACAGCTTTG GTGTGGTTTT ACTAGAAATA
ATAACTGGAC TTCCAGCTGT GGATGAACAC CGTGAACCTC AGTTATTGCT AGATATTAAA
GAAGAAATTG AAGATGAAGA AAAGACAATT GAAGATTATA TTGATAAAAA GATGAATGAT
GCTGATTCCA CTTCAGTTGA AGCTATGTAC TCTGTTGCTA GTCAATGTCT GCATGAAAAG
AAAAATAAGA GACCAGACAT TAAGAAGGTT CAACAGCTGC TGCAAGAGAT GACAGCTTCT
TAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu15137D
Sequence Information ORF Nucleotide Sequence (Length: 1383bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product interleukin-1 receptor-associated kinase 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAACAAAC CCATAACACC ATCAACATAT GTGCGCTGCC TCAATGTTGG ACTAATTAGG 
AAGCTGTCAG ATTTTATTGA TCCTCAAGAA GGATGGAAGA AGTTAGCTGT AGCTATTAAA
AAACCATCTG GTGATGATAG ATACAATCAG TTTCACATAA GGAGATTTGA AGCATTACTT
CAAACTGGAA AAAGTCCCAC TTCTGAATTA CTGTTTGACT GGGGCACCAC AAATTGCACA
GTTGGTGATC TTGTGGATCT TTTGATCCAA AATGAATTTT TTGCTCCTGC GAGTCTTTTG
CTCCCAGATG CTGTTCCCAA AACTGCTAAT ACACTACCTT CTAAAGAAGC TATAACAGTT
CAGCAAAAAC AGATGCCTTT CTGTGACAAA GACAGGACAT TGATGACACC TGTGCAGAAT
CTTGAACAAA GCTATATGCC ACCTGACTCC TCAAGTCCAG AAAATAAAAG TTTAGAAGTT
AGTGATACAC GTTTTCACAG TTTTTCATTT TATGAATTGA AGAATGTCAC AAATAACTTT
GATGAACGAC CCATTTCTGT TGGTGGTAAT AAAATGGGAG AGGGAGGATT TGGAGTTGTA
TATAAAGGCT ACGTAAATAA CACAACTGTG GCAGTGAAGA AGCTTGCAGC AATGGTTGAC
ATTACTACTG AAGAACTGAA ACAGCAGTTT GATCAAGAAA TAAAAGTAAT GGCAAAGTGT
CAACATGAAA ACTTAGTAGA ACTACTTGGT TTCTCAAGTG ATGGAGATGA CCTCTGCTTA
GTATATGTTT ACATGCCTAA TGGTTCATTG CTAGACAGAC TCTCTTGCTT GGATGGTACT
CCACCACTTT CTTGGCACAT GAGATGCAAG ATTGCTCAGG GTGCAGCTAA TGGCATCAAT
TTTCTACATG AAAATCATCA TATTCATAGA GATATTAAAA GTGCAAATAT CTTACTGGAT
GAAGCTTTTA CTGCTAAAAT ATCTGACTTT GGCCTTGCAC GGGCTTCTGA GAAGTTTGCC
CAGACAGTCA TGACTAGCAG AATTGTGGGA ACAACAGCTT ATATGGCACC AGAAGCTTTG
CGTGGAGAAA TAACACCCAA ATCTGATATT TACAGCTTTG GTGTGGTTTT ACTAGAAATA
ATAACTGGAC TTCCAGCTGT GGATGAACAC CGTGAACCTC AGTTATTGCT AGATATTAAA
GAAGAAATTG AAGATGAAGA AAAGACAATT GAAGATTATA TTGATAAAAA GATGAATGAT
GCTGATTCCA CTTCAGTTGA AGCTATGTAC TCTGTTGCTA GTCAATGTCT GCATGAAAAG
AAAAATAAGA GACCAGACAT TAAGAAGGTT CAACAGCTGC TGCAAGAGAT GACAGCTTCT
TAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011536734.1
CDS236..1618
Misc Feature(1)260..553(+)
Misc Feature(2)269..469(+)
Misc Feature(3)308..529(+)
Misc Feature(4)743..1606(+)
Misc Feature(5)800..1597(+)
Misc Feature(6)809..1294(+)
Misc Feature(7)809..1222(+)
Misc Feature(8)821..1294(+)
Misc Feature(9)1217..1294(+)
Translation
Position Chain Variation Link
37 37 c, g dbSNP:770034521
48 48 a, c dbSNP:189919547
57 57 a, g dbSNP:773753761
61 61 c, t dbSNP:530898596
83 83 g, t dbSNP:549501045
99 99 a, g dbSNP:567654648
113 113 c, t dbSNP:528426421
175 175 -, t dbSNP:765330474
181 181 g, t dbSNP:555695963
199 199 c, g dbSNP:4251432
240 240 a, g dbSNP:561048547
242 242 a, c dbSNP:752890848
246 246 c, t dbSNP:750912907
248 248 a, g dbSNP:56312115
251 251 a, c dbSNP:780540482
256 256 a, g dbSNP:201400143
262 262 a, c dbSNP:749572231
269 269 c, t dbSNP:377584435
270 270 a, g dbSNP:370524518
275 275 c, t dbSNP:748441973
279 279 a, g dbSNP:772274120
285 285 a, g dbSNP:778057572
289 289 a, g dbSNP:747100232
293 293 a, t dbSNP:143625818
298 298 a, g dbSNP:775601867
301 301 a, g dbSNP:762610309
311 311 a, g dbSNP:768548402
312 312 c, t dbSNP:138116867
317 317 a, c dbSNP:761596704
334 334 a, g dbSNP:115877973
347 347 a, c, g dbSNP:565544986
350 350 a, g dbSNP:113588409
352 352 a, t dbSNP:141039652
358 358 a, g dbSNP:551138584
366 366 g, t dbSNP:755265254
379 379 a, c dbSNP:779400593
384 384 a, t dbSNP:752850989
389 389 c, g dbSNP:758634981
397 397 a, g dbSNP:772625246
418 418 a, c dbSNP:778094168
420 420 c, g dbSNP:751881820
423 423 c, g dbSNP:572719034
441 441 a, c dbSNP:202134282
454 454 c, g, t dbSNP:374971450
458 458 a, g dbSNP:778743146
462 462 c, t dbSNP:200175187
465 465 a, c dbSNP:771900851
466 466 a, c dbSNP:772817746
479 479 a, g dbSNP:746729422
483 483 a, g dbSNP:770333429
487 487 c, t dbSNP:531518356
499 499 a, g dbSNP:776148970
501 501 c, t dbSNP:758974509
503 503 c, t dbSNP:765736285
508 508 c, t dbSNP:775885677
522 522 c, t dbSNP:763390934
525 525 c, t dbSNP:764597369
526 526 a, g dbSNP:17851028
529 529 g, t dbSNP:4251469
532 532 g, t dbSNP:757615268
538 538 c, t dbSNP:371634312
543 543 a, c, g dbSNP:774802371
549 549 -, tt dbSNP:763078267
553 553 c, g dbSNP:56338336
561 561 c, g dbSNP:750658320
568 568 -, a dbSNP:766515269
569 569 c, t dbSNP:756185480
578 578 a, g dbSNP:766433748
580 580 a, g dbSNP:752616965
581 581 a, g dbSNP:62642475
587 587 a, g dbSNP:540605517
606 606 a, c dbSNP:771105240
618 618 g, t dbSNP:777691189
624 624 a, g dbSNP:751316240
627 627 a, c dbSNP:757025762
634 634 a, g dbSNP:780877191
638 638 a, g dbSNP:745549223
641 641 a, g dbSNP:769345767
642 642 c, t dbSNP:779771826
649 649 a, g dbSNP:748825445
650 650 c, t dbSNP:769242383
658 658 c, t dbSNP:775016898
659 659 c, g dbSNP:762213099
660 660 a, t dbSNP:772359426
662 662 a, c dbSNP:773476797
666 666 c, g dbSNP:147079926
667 667 c, t dbSNP:766601065
672 672 g, t dbSNP:753847808
680 680 c, g dbSNP:375927287
681 681 a, g dbSNP:112228761
682 682 -, ctc dbSNP:556809469
688 688 -, a dbSNP:760054904
691 691 c, t dbSNP:763971241
697 697 a, t dbSNP:751549092
699 699 a, c dbSNP:757047226
702 702 a, g dbSNP:780919244
704 704 a, t dbSNP:750080879
705 705 g, t dbSNP:755668007
709 709 a, g dbSNP:779861617
711 711 a, t dbSNP:748754154
714 714 a, t dbSNP:201782959
719 719 a, g dbSNP:779528103
724 724 a, g, t dbSNP:748667787
740 740 c, t dbSNP:778367357
741 741 c, t dbSNP:747296110
757 757 g, t dbSNP:771324378
763 763 a, c dbSNP:776810085
764 764 a, g dbSNP:141209982
769 769 c, t dbSNP:769857009
772 772 c, g dbSNP:373731553
773 773 -, t dbSNP:753106997
782 782 c, t dbSNP:114951157
783 783 a, g dbSNP:186194251
796 796 c, t dbSNP:137949295
827 827 c, g dbSNP:773245379
827 827 -, g dbSNP:756802570
829 829 a, t dbSNP:113948349
833 833 c, g dbSNP:377074100
840 840 a, g dbSNP:191097365
847 847 c, t dbSNP:753538997
848 848 a, g dbSNP:370225662
873 873 a, t dbSNP:149453390
894 894 a, g dbSNP:781671136
903 903 c, t dbSNP:750733250
905 905 a, g dbSNP:201500711
913 913 a, g dbSNP:780121221
926 926 a, g dbSNP:111935971
932 932 a, g dbSNP:200082169
937 937 a, g, t dbSNP:778986995
940 940 a, t dbSNP:748253425
947 947 g, t dbSNP:770943951
955 955 c, t dbSNP:144854858
966 966 a, g dbSNP:781252640
977 977 c, t dbSNP:375755560
984 984 a, g dbSNP:769470855
993 993 c, g dbSNP:775286298
995 995 g, t dbSNP:762667477
996 996 a, t dbSNP:768303231
998 998 a, g dbSNP:774020314
1001 1001 a, g dbSNP:761277223
1006 1006 c, t dbSNP:767075679
1020 1020 a, g dbSNP:773708174
1021 1021 c, t dbSNP:201704200
1022 1022 a, g dbSNP:112517169
1026 1026 a, t dbSNP:766812248
1042 1042 a, c dbSNP:754184412
1054 1054 a, t dbSNP:370042548
1057 1057 c, g, t dbSNP:755330743
1060 1060 c, t dbSNP:140210540
1067 1067 a, g dbSNP:757311009
1068 1068 a, g, t dbSNP:780005506
1069 1069 c, t dbSNP:754767022
1085 1085 c, t dbSNP:778616067
1090 1090 g, t dbSNP:747953616
1095 1095 g, t dbSNP:771667566
1100 1100 c, t dbSNP:373167326
1105 1105 c, g dbSNP:145622820
1109 1109 c, g dbSNP:770396929
1112 1112 c, t dbSNP:121908002
1113 1113 a, g dbSNP:760186666
1115 1115 a, g dbSNP:200883675
1116 1116 a, g dbSNP:775937250
1124 1124 a, g dbSNP:556565376
1128 1128 a, g, t dbSNP:568782766
1134 1134 a, g dbSNP:376948391
1135 1135 c, t dbSNP:536101269
1141 1141 a, t dbSNP:750525615
1151 1151 a, c dbSNP:115573494
1152 1152 a, t dbSNP:778846957
1158 1158 a, t dbSNP:752451445
1159 1159 a, t dbSNP:374532899
1170 1170 c, t dbSNP:777460833
1178 1178 a, g dbSNP:751373844
1185 1185 a, t dbSNP:756802579
1196 1196 a, g dbSNP:780917972
1197 1197 a, t dbSNP:147301051
1200 1200 c, g dbSNP:769465559
1208 1208 a, g dbSNP:780681951
1225 1225 c, t dbSNP:749864140
1227 1227 g, t dbSNP:769214804
1235 1235 c, t dbSNP:774525787
1236 1236 a, g dbSNP:748570560
1253 1253 g, t dbSNP:772148472
1264 1264 c, t dbSNP:773464920
1272 1272 a, g dbSNP:368108618
1292 1292 a, g dbSNP:140869783
1298 1298 a, c, g dbSNP:142376871
1308 1308 a, t dbSNP:775402267
1316 1316 c, t dbSNP:762731086
1317 1317 a, g dbSNP:764106350
1319 1319 a, g dbSNP:751261930
1333 1333 c, t dbSNP:757104944
1344 1344 c, t dbSNP:372448559
1346 1346 g, t dbSNP:750146218
1355 1355 a, g dbSNP:779468411
1358 1358 a, g dbSNP:755668302
1359 1359 c, t dbSNP:779773825
1377 1377 c, t dbSNP:767476422
1380 1380 c, g dbSNP:750092963
1381 1381 -, tg dbSNP:764404378
1383 1383 a, g, t dbSNP:755772831
1384 1384 a, g dbSNP:753251856
1396 1396 a, g dbSNP:757918302
1398 1398 a, t dbSNP:151272504
1404 1404 a, g dbSNP:4251583
1406 1406 c, t dbSNP:114820168
1407 1407 a, g dbSNP:55944915
1412 1412 c, g dbSNP:758688655
1426 1426 a, g dbSNP:773095217
1434 1434 -, aag dbSNP:758148548
1436 1436 a, g dbSNP:760580944
1439 1439 g, t dbSNP:766102187
1457 1457 a, c dbSNP:547810966
1460 1460 a, g dbSNP:758956283
1463 1463 a, g dbSNP:764868568
1464 1464 c, t dbSNP:146538989
1475 1475 a, g dbSNP:758846627
1476 1476 c, t dbSNP:778274322
1496 1496 a, g dbSNP:751879000
1514 1514 a, g dbSNP:757657887
1517 1517 a, g dbSNP:4251545
1521 1521 c, t dbSNP:533369384
1526 1526 g, t dbSNP:769754150
1528 1528 g, t dbSNP:780112532
1529 1529 c, g dbSNP:749338213
1532 1532 a, g dbSNP:772098333
1533 1533 c, g dbSNP:773326632
1536 1536 c, g dbSNP:760527974
1537 1537 c, t dbSNP:551524673
1541 1541 a, t dbSNP:770908857
1542 1542 c, g dbSNP:776143317
1546 1546 a, g dbSNP:759164006
1549 1549 c, t dbSNP:144842940
1564 1564 c, g dbSNP:752201648
1566 1566 g, t dbSNP:780633823
1568 1568 a, c, t dbSNP:762479548
1569 1569 a, c dbSNP:752108446
1573 1573 c, t dbSNP:138719119
1575 1575 a, g, t dbSNP:569421834
1579 1579 g, t dbSNP:756298129
1586 1586 c, g dbSNP:762236455
1600 1600 a, g dbSNP:767921421
1601 1601 a, g dbSNP:148873968
1602 1602 a, t dbSNP:372068514
1606 1606 a, g dbSNP:766606181
1608 1608 c, t dbSNP:754175088
1622 1622 c, t dbSNP:755104443
1625 1625 a, g dbSNP:567188868
1635 1635 a, t dbSNP:748253330
1637 1637 c, t dbSNP:199727839
1642 1642 a, g dbSNP:781021520
1645 1645 c, t dbSNP:781731425
1651 1651 a, g dbSNP:745643347
1659 1659 a, g dbSNP:553330681
1661 1661 c, g dbSNP:769526114
1669 1669 -, t dbSNP:755909125
1670 1670 a, t dbSNP:184380424
1730 1730 a, g dbSNP:577974384
1764 1764 c, t dbSNP:4251546
1768 1768 a, g dbSNP:182322900
1863 1863 a, g dbSNP:187201106
1888 1888 c, t dbSNP:4251547
1899 1899 a, g dbSNP:4251548
1923 1923 g, t dbSNP:115845154
1937 1937 a, g dbSNP:769535835
1960 1960 g, t dbSNP:147004395
1961 1961 a, g dbSNP:770150730
2015 2015 a, g dbSNP:773095431
2028 2028 a, g dbSNP:559634238
2046 2046 a, t dbSNP:4251549
2080 2080 c, t dbSNP:191617315
2106 2106 a, t dbSNP:183559035
2138 2138 a, t dbSNP:4251550
2140 2140 a, c dbSNP:548838962
2226 2226 a, g dbSNP:4251551
2238 2238 a, g dbSNP:138178820
2241 2241 a, g dbSNP:4251552
2256 2256 a, c dbSNP:571568921
2284 2284 g, t dbSNP:774327325
2288 2288 g, t dbSNP:759513055
2299 2299 a, g dbSNP:538982204
2386 2386 c, t dbSNP:576622168
2436 2436 a, t dbSNP:767298091
2458 2458 a, c dbSNP:752547785
2467 2467 -, a dbSNP:36085436
2472 2472 a, g dbSNP:567630494
2494 2494 a, g dbSNP:566365168
2574 2574 a, g dbSNP:575385169
2585 2585 a, g dbSNP:542787354
2607 2607 g, t dbSNP:4251584
2685 2685 a, g dbSNP:574043929
2694 2694 c, t dbSNP:541034481
2714 2714 a, c dbSNP:559273571
2720 2720 a, g dbSNP:753237737
2725 2725 a, c dbSNP:777619096
2726 2726 a, g dbSNP:141847763
2779 2779 a, g dbSNP:545334973
2788 2788 c, t dbSNP:563991845
2805 2805 c, t dbSNP:755598323
2816 2816 a, c dbSNP:531038940
2829 2829 a, g dbSNP:781639576
2890 2890 g, t dbSNP:182497864
2891 2891 c, t dbSNP:111571285
2907 2907 a, c dbSNP:150604439
2914 2914 c, t dbSNP:528147840
2920 2920 a, c dbSNP:554628455
2924 2924 c, t dbSNP:770097373
2943 2943 a, g dbSNP:139778399
2947 2947 a, g dbSNP:563645022
2957 2957 -, ataa dbSNP:138875923
2958 2958 -, ataa dbSNP:527750692
2961 2961 -, ataa dbSNP:531640584
2976 2976 -, aaat dbSNP:368676868
2982 2982 g, t dbSNP:186825265
3004 3004 a, g dbSNP:530704544
3019 3019 g, t dbSNP:4251553
3133 3133 c, t dbSNP:536073958
3142 3142 c, t dbSNP:554707596
3143 3143 a, g dbSNP:542838156
3152 3152 a, g dbSNP:574051595
3161 3161 a, c, g dbSNP:139675032
3206 3206 a, g dbSNP:778083287
3225 3225 a, t dbSNP:553037446
3286 3286 c, g dbSNP:576135948
3300 3300 g, t dbSNP:192534409
3306 3306 -, g dbSNP:778897449
3310 3310 c, t dbSNP:544979835
3314 3314 a, g dbSNP:543426658
3337 3337 a, g dbSNP:771014920
3371 3371 c, t dbSNP:558662153
3378 3378 a, g dbSNP:144535012
3395 3395 a, t dbSNP:4251554
3412 3412 c, t dbSNP:4251555
3442 3442 -, ctt dbSNP:79794889
3443 3443 -, t dbSNP:538947924
3494 3494 a, g dbSNP:528687727
3501 3501 a, g dbSNP:546754318
3505 3505 c, t dbSNP:4251556
3506 3506 a, g dbSNP:183931852
3511 3511 c, t dbSNP:4251557
3513 3513 a, g dbSNP:4251558
3551 3551 a, c dbSNP:536497843
3559 3559 a, c dbSNP:548182708
3592 3592 a, g dbSNP:12321678
3632 3632 a, g dbSNP:533723416
3658 3658 a, g dbSNP:371821781
3679 3679 c, g dbSNP:533412197
3689 3689 c, t dbSNP:776314485
3698 3698 c, t dbSNP:776764561
3701 3701 c, g dbSNP:111813306
3718 3718 a, g dbSNP:534645871
3741 3741 g, t dbSNP:374721629
3806 3806 a, g dbSNP:1141168
3816 3816 c, t dbSNP:563217241
3841 3841 g, t dbSNP:769976979
3873 3873 g, t dbSNP:557276770
3884 3884 a, g dbSNP:4251560
3919 3919 g, t dbSNP:543346753
3963 3963 a, g dbSNP:368152755
3967 3967 c, t dbSNP:749964168
3976 3976 g, t dbSNP:554949427
4025 4025 -, tgtttaga dbSNP:747864158
4051 4051 c, t dbSNP:9849
4162 4162 a, g dbSNP:76405665
4180 4180 c, g, t dbSNP:145856072
4181 4181 a, g dbSNP:756483703
4183 4183 a, g dbSNP:549897461
4195 4195 g, t dbSNP:549623720
4230 4230 c, t dbSNP:147831464
4283 4283 c, t dbSNP:532207331
4284 4284 a, g, t dbSNP:375914722
4287 4287 c, t dbSNP:12310328
4320 4320 a, g dbSNP:749524568
4322 4322 c, t dbSNP:4251562
4343 4343 c, g dbSNP:766712571
4370 4370 c, t dbSNP:369823166
4371 4371 a, g dbSNP:562549392
4379 4379 a, g dbSNP:4251563
4422 4422 c, t dbSNP:771957948
4435 4435 a, g dbSNP:4251564

Target ORF information:

RefSeq Version XM_011538432
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011538432

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAACAAAC CCATAACACC ATCAACATAT GTGCGCTGCC TCAATGTTGG ACTAATTAGG 
AAGCTGTCAG ATTTTATTGA TCCTCAAGAA GGATGGAAGA AGTTAGCTGT AGCTATTAAA
AAACCATCTG GTGATGATAG ATACAATCAG TTTCACATAA GGAGATTTGA AGCATTACTT
CAAACTGGAA AAAGTCCCAC TTCTGAATTA CTGTTTGACT GGGGCACCAC AAATTGCACA
GTTGGTGATC TTGTGGATCT TTTGATCCAA AATGAATTTT TTGCTCCTGC GAGTCTTTTG
CTCCCAGATG CTGTTCCCAA AACTGCTAAT ACACTACCTT CTAAAGAAGC TATAACAGTT
CAGCAAAAAC AGATGCCTTT CTGTGACAAA GACAGGACAT TGATGACACC TGTGCAGAAT
CTTGAACAAA GCTATATGCC ACCTGACTCC TCAAGTCCAG AAAATAAAAG TTTAGAAGTT
AGTGATACAC GTTTTCACAG TTTTTCATTT TATGAATTGA AGAATGTCAC AAATAACTTT
GATGAACGAC CCATTTCTGT TGGTGGTAAT AAAATGGGAG AGGGAGGATT TGGAGTTGTA
TATAAAGGCT ACGTAAATAA CACAACTGTG GCAGTGAAGA AGCTTGCAGC AATGGTTGAC
ATTACTACTG AAGAACTGAA ACAGCAGTTT GATCAAGAAA TAAAAGTAAT GGCAAAGTGT
CAACATGAAA ACTTAGTAGA ACTACTTGGT TTCTCAAGTG ATGGAGATGA CCTCTGCTTA
GTATATGTTT ACATGCCTAA TGGTTCATTG CTAGACAGAC TCTCTTGCTT GGATGGTACT
CCACCACTTT CTTGGCACAT GAGATGCAAG ATTGCTCAGG GTGCAGCTAA TGGCATCAAT
TTTCTACATG AAAATCATCA TATTCATAGA GATATTAAAA GTGCAAATAT CTTACTGGAT
GAAGCTTTTA CTGCTAAAAT ATCTGACTTT GGCCTTGCAC GGGCTTCTGA GAAGTTTGCC
CAGACAGTCA TGACTAGCAG AATTGTGGGA ACAACAGCTT ATATGGCACC AGAAGCTTTG
CGTGGAGAAA TAACACCCAA ATCTGATATT TACAGCTTTG GTGTGGTTTT ACTAGAAATA
ATAACTGGAC TTCCAGCTGT GGATGAACAC CGTGAACCTC AGTTATTGCT AGATATTAAA
GAAGAAATTG AAGATGAAGA AAAGACAATT GAAGATTATA TTGATAAAAA GATGAATGAT
GCTGATTCCA CTTCAGTTGA AGCTATGTAC TCTGTTGCTA GTCAATGTCT GCATGAAAAG
AAAAATAAGA GACCAGACAT TAAGAAGGTT CAACAGCTGC TGCAAGAGAT GACAGCTTCT
TAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu15137D
Sequence Information ORF Nucleotide Sequence (Length: 1383bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product interleukin-1 receptor-associated kinase 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAACAAAC CCATAACACC ATCAACATAT GTGCGCTGCC TCAATGTTGG ACTAATTAGG 
AAGCTGTCAG ATTTTATTGA TCCTCAAGAA GGATGGAAGA AGTTAGCTGT AGCTATTAAA
AAACCATCTG GTGATGATAG ATACAATCAG TTTCACATAA GGAGATTTGA AGCATTACTT
CAAACTGGAA AAAGTCCCAC TTCTGAATTA CTGTTTGACT GGGGCACCAC AAATTGCACA
GTTGGTGATC TTGTGGATCT TTTGATCCAA AATGAATTTT TTGCTCCTGC GAGTCTTTTG
CTCCCAGATG CTGTTCCCAA AACTGCTAAT ACACTACCTT CTAAAGAAGC TATAACAGTT
CAGCAAAAAC AGATGCCTTT CTGTGACAAA GACAGGACAT TGATGACACC TGTGCAGAAT
CTTGAACAAA GCTATATGCC ACCTGACTCC TCAAGTCCAG AAAATAAAAG TTTAGAAGTT
AGTGATACAC GTTTTCACAG TTTTTCATTT TATGAATTGA AGAATGTCAC AAATAACTTT
GATGAACGAC CCATTTCTGT TGGTGGTAAT AAAATGGGAG AGGGAGGATT TGGAGTTGTA
TATAAAGGCT ACGTAAATAA CACAACTGTG GCAGTGAAGA AGCTTGCAGC AATGGTTGAC
ATTACTACTG AAGAACTGAA ACAGCAGTTT GATCAAGAAA TAAAAGTAAT GGCAAAGTGT
CAACATGAAA ACTTAGTAGA ACTACTTGGT TTCTCAAGTG ATGGAGATGA CCTCTGCTTA
GTATATGTTT ACATGCCTAA TGGTTCATTG CTAGACAGAC TCTCTTGCTT GGATGGTACT
CCACCACTTT CTTGGCACAT GAGATGCAAG ATTGCTCAGG GTGCAGCTAA TGGCATCAAT
TTTCTACATG AAAATCATCA TATTCATAGA GATATTAAAA GTGCAAATAT CTTACTGGAT
GAAGCTTTTA CTGCTAAAAT ATCTGACTTT GGCCTTGCAC GGGCTTCTGA GAAGTTTGCC
CAGACAGTCA TGACTAGCAG AATTGTGGGA ACAACAGCTT ATATGGCACC AGAAGCTTTG
CGTGGAGAAA TAACACCCAA ATCTGATATT TACAGCTTTG GTGTGGTTTT ACTAGAAATA
ATAACTGGAC TTCCAGCTGT GGATGAACAC CGTGAACCTC AGTTATTGCT AGATATTAAA
GAAGAAATTG AAGATGAAGA AAAGACAATT GAAGATTATA TTGATAAAAA GATGAATGAT
GCTGATTCCA CTTCAGTTGA AGCTATGTAC TCTGTTGCTA GTCAATGTCT GCATGAAAAG
AAAAATAAGA GACCAGACAT TAAGAAGGTT CAACAGCTGC TGCAAGAGAT GACAGCTTCT
TAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011536735.1
CDS249..1631
Misc Feature(1)273..566(+)
Misc Feature(2)282..482(+)
Misc Feature(3)321..542(+)
Misc Feature(4)756..1619(+)
Misc Feature(5)813..1610(+)
Misc Feature(6)822..1307(+)
Misc Feature(7)822..1235(+)
Misc Feature(8)834..1307(+)
Misc Feature(9)1230..1307(+)
Translation
Position Chain Variation Link
12 12 c, t dbSNP:527844708
13 13 a, c dbSNP:552817462
23 23 -, a dbSNP:35671691
59 59 c, g dbSNP:570532333
68 68 c, t dbSNP:566213725
73 73 c, g dbSNP:530094138
99 99 c, t dbSNP:4251569
129 129 g, t dbSNP:140402035
140 140 a, g dbSNP:149690962
165 165 a, c dbSNP:779428716
213 213 c, t dbSNP:187147690
222 222 -, a dbSNP:755886097
253 253 a, g dbSNP:561048547
255 255 a, c dbSNP:752890848
259 259 c, t dbSNP:750912907
261 261 a, g dbSNP:56312115
264 264 a, c dbSNP:780540482
269 269 a, g dbSNP:201400143
275 275 a, c dbSNP:749572231
282 282 c, t dbSNP:377584435
283 283 a, g dbSNP:370524518
288 288 c, t dbSNP:748441973
292 292 a, g dbSNP:772274120
298 298 a, g dbSNP:778057572
302 302 a, g dbSNP:747100232
306 306 a, t dbSNP:143625818
311 311 a, g dbSNP:775601867
314 314 a, g dbSNP:762610309
324 324 a, g dbSNP:768548402
325 325 c, t dbSNP:138116867
330 330 a, c dbSNP:761596704
347 347 a, g dbSNP:115877973
360 360 a, c, g dbSNP:565544986
363 363 a, g dbSNP:113588409
365 365 a, t dbSNP:141039652
371 371 a, g dbSNP:551138584
379 379 g, t dbSNP:755265254
392 392 a, c dbSNP:779400593
397 397 a, t dbSNP:752850989
402 402 c, g dbSNP:758634981
410 410 a, g dbSNP:772625246
431 431 a, c dbSNP:778094168
433 433 c, g dbSNP:751881820
436 436 c, g dbSNP:572719034
454 454 a, c dbSNP:202134282
467 467 c, g, t dbSNP:374971450
471 471 a, g dbSNP:778743146
475 475 c, t dbSNP:200175187
478 478 a, c dbSNP:771900851
479 479 a, c dbSNP:772817746
492 492 a, g dbSNP:746729422
496 496 a, g dbSNP:770333429
500 500 c, t dbSNP:531518356
512 512 a, g dbSNP:776148970
514 514 c, t dbSNP:758974509
516 516 c, t dbSNP:765736285
521 521 c, t dbSNP:775885677
535 535 c, t dbSNP:763390934
538 538 c, t dbSNP:764597369
539 539 a, g dbSNP:17851028
542 542 g, t dbSNP:4251469
545 545 g, t dbSNP:757615268
551 551 c, t dbSNP:371634312
556 556 a, c, g dbSNP:774802371
562 562 -, tt dbSNP:763078267
566 566 c, g dbSNP:56338336
574 574 c, g dbSNP:750658320
581 581 -, a dbSNP:766515269
582 582 c, t dbSNP:756185480
591 591 a, g dbSNP:766433748
593 593 a, g dbSNP:752616965
594 594 a, g dbSNP:62642475
600 600 a, g dbSNP:540605517
619 619 a, c dbSNP:771105240
631 631 g, t dbSNP:777691189
637 637 a, g dbSNP:751316240
640 640 a, c dbSNP:757025762
647 647 a, g dbSNP:780877191
651 651 a, g dbSNP:745549223
654 654 a, g dbSNP:769345767
655 655 c, t dbSNP:779771826
662 662 a, g dbSNP:748825445
663 663 c, t dbSNP:769242383
671 671 c, t dbSNP:775016898
672 672 c, g dbSNP:762213099
673 673 a, t dbSNP:772359426
675 675 a, c dbSNP:773476797
679 679 c, g dbSNP:147079926
680 680 c, t dbSNP:766601065
685 685 g, t dbSNP:753847808
693 693 c, g dbSNP:375927287
694 694 a, g dbSNP:112228761
695 695 -, ctc dbSNP:556809469
701 701 -, a dbSNP:760054904
704 704 c, t dbSNP:763971241
710 710 a, t dbSNP:751549092
712 712 a, c dbSNP:757047226
715 715 a, g dbSNP:780919244
717 717 a, t dbSNP:750080879
718 718 g, t dbSNP:755668007
722 722 a, g dbSNP:779861617
724 724 a, t dbSNP:748754154
727 727 a, t dbSNP:201782959
732 732 a, g dbSNP:779528103
737 737 a, g, t dbSNP:748667787
753 753 c, t dbSNP:778367357
754 754 c, t dbSNP:747296110
770 770 g, t dbSNP:771324378
776 776 a, c dbSNP:776810085
777 777 a, g dbSNP:141209982
782 782 c, t dbSNP:769857009
785 785 c, g dbSNP:373731553
786 786 -, t dbSNP:753106997
795 795 c, t dbSNP:114951157
796 796 a, g dbSNP:186194251
809 809 c, t dbSNP:137949295
840 840 c, g dbSNP:773245379
840 840 -, g dbSNP:756802570
842 842 a, t dbSNP:113948349
846 846 c, g dbSNP:377074100
853 853 a, g dbSNP:191097365
860 860 c, t dbSNP:753538997
861 861 a, g dbSNP:370225662
886 886 a, t dbSNP:149453390
907 907 a, g dbSNP:781671136
916 916 c, t dbSNP:750733250
918 918 a, g dbSNP:201500711
926 926 a, g dbSNP:780121221
939 939 a, g dbSNP:111935971
945 945 a, g dbSNP:200082169
950 950 a, g, t dbSNP:778986995
953 953 a, t dbSNP:748253425
960 960 g, t dbSNP:770943951
968 968 c, t dbSNP:144854858
979 979 a, g dbSNP:781252640
990 990 c, t dbSNP:375755560
997 997 a, g dbSNP:769470855
1006 1006 c, g dbSNP:775286298
1008 1008 g, t dbSNP:762667477
1009 1009 a, t dbSNP:768303231
1011 1011 a, g dbSNP:774020314
1014 1014 a, g dbSNP:761277223
1019 1019 c, t dbSNP:767075679
1033 1033 a, g dbSNP:773708174
1034 1034 c, t dbSNP:201704200
1035 1035 a, g dbSNP:112517169
1039 1039 a, t dbSNP:766812248
1055 1055 a, c dbSNP:754184412
1067 1067 a, t dbSNP:370042548
1070 1070 c, g, t dbSNP:755330743
1073 1073 c, t dbSNP:140210540
1080 1080 a, g dbSNP:757311009
1081 1081 a, g, t dbSNP:780005506
1082 1082 c, t dbSNP:754767022
1098 1098 c, t dbSNP:778616067
1103 1103 g, t dbSNP:747953616
1108 1108 g, t dbSNP:771667566
1113 1113 c, t dbSNP:373167326
1118 1118 c, g dbSNP:145622820
1122 1122 c, g dbSNP:770396929
1125 1125 c, t dbSNP:121908002
1126 1126 a, g dbSNP:760186666
1128 1128 a, g dbSNP:200883675
1129 1129 a, g dbSNP:775937250
1137 1137 a, g dbSNP:556565376
1141 1141 a, g, t dbSNP:568782766
1147 1147 a, g dbSNP:376948391
1148 1148 c, t dbSNP:536101269
1154 1154 a, t dbSNP:750525615
1164 1164 a, c dbSNP:115573494
1165 1165 a, t dbSNP:778846957
1171 1171 a, t dbSNP:752451445
1172 1172 a, t dbSNP:374532899
1183 1183 c, t dbSNP:777460833
1191 1191 a, g dbSNP:751373844
1198 1198 a, t dbSNP:756802579
1209 1209 a, g dbSNP:780917972
1210 1210 a, t dbSNP:147301051
1213 1213 c, g dbSNP:769465559
1221 1221 a, g dbSNP:780681951
1238 1238 c, t dbSNP:749864140
1240 1240 g, t dbSNP:769214804
1248 1248 c, t dbSNP:774525787
1249 1249 a, g dbSNP:748570560
1266 1266 g, t dbSNP:772148472
1277 1277 c, t dbSNP:773464920
1285 1285 a, g dbSNP:368108618
1305 1305 a, g dbSNP:140869783
1311 1311 a, c, g dbSNP:142376871
1321 1321 a, t dbSNP:775402267
1329 1329 c, t dbSNP:762731086
1330 1330 a, g dbSNP:764106350
1332 1332 a, g dbSNP:751261930
1346 1346 c, t dbSNP:757104944
1357 1357 c, t dbSNP:372448559
1359 1359 g, t dbSNP:750146218
1368 1368 a, g dbSNP:779468411
1371 1371 a, g dbSNP:755668302
1372 1372 c, t dbSNP:779773825
1390 1390 c, t dbSNP:767476422
1393 1393 c, g dbSNP:750092963
1394 1394 -, tg dbSNP:764404378
1396 1396 a, g, t dbSNP:755772831
1397 1397 a, g dbSNP:753251856
1409 1409 a, g dbSNP:757918302
1411 1411 a, t dbSNP:151272504
1417 1417 a, g dbSNP:4251583
1419 1419 c, t dbSNP:114820168
1420 1420 a, g dbSNP:55944915
1425 1425 c, g dbSNP:758688655
1439 1439 a, g dbSNP:773095217
1447 1447 -, aag dbSNP:758148548
1449 1449 a, g dbSNP:760580944
1452 1452 g, t dbSNP:766102187
1470 1470 a, c dbSNP:547810966
1473 1473 a, g dbSNP:758956283
1476 1476 a, g dbSNP:764868568
1477 1477 c, t dbSNP:146538989
1488 1488 a, g dbSNP:758846627
1489 1489 c, t dbSNP:778274322
1509 1509 a, g dbSNP:751879000
1527 1527 a, g dbSNP:757657887
1530 1530 a, g dbSNP:4251545
1534 1534 c, t dbSNP:533369384
1539 1539 g, t dbSNP:769754150
1541 1541 g, t dbSNP:780112532
1542 1542 c, g dbSNP:749338213
1545 1545 a, g dbSNP:772098333
1546 1546 c, g dbSNP:773326632
1549 1549 c, g dbSNP:760527974
1550 1550 c, t dbSNP:551524673
1554 1554 a, t dbSNP:770908857
1555 1555 c, g dbSNP:776143317
1559 1559 a, g dbSNP:759164006
1562 1562 c, t dbSNP:144842940
1577 1577 c, g dbSNP:752201648
1579 1579 g, t dbSNP:780633823
1581 1581 a, c, t dbSNP:762479548
1582 1582 a, c dbSNP:752108446
1586 1586 c, t dbSNP:138719119
1588 1588 a, g, t dbSNP:569421834
1592 1592 g, t dbSNP:756298129
1599 1599 c, g dbSNP:762236455
1613 1613 a, g dbSNP:767921421
1614 1614 a, g dbSNP:148873968
1615 1615 a, t dbSNP:372068514
1619 1619 a, g dbSNP:766606181
1621 1621 c, t dbSNP:754175088
1635 1635 c, t dbSNP:755104443
1638 1638 a, g dbSNP:567188868
1648 1648 a, t dbSNP:748253330
1650 1650 c, t dbSNP:199727839
1655 1655 a, g dbSNP:781021520
1658 1658 c, t dbSNP:781731425
1664 1664 a, g dbSNP:745643347
1672 1672 a, g dbSNP:553330681
1674 1674 c, g dbSNP:769526114
1682 1682 -, t dbSNP:755909125
1683 1683 a, t dbSNP:184380424
1743 1743 a, g dbSNP:577974384
1777 1777 c, t dbSNP:4251546
1781 1781 a, g dbSNP:182322900
1876 1876 a, g dbSNP:187201106
1901 1901 c, t dbSNP:4251547
1912 1912 a, g dbSNP:4251548
1936 1936 g, t dbSNP:115845154
1950 1950 a, g dbSNP:769535835
1973 1973 g, t dbSNP:147004395
1974 1974 a, g dbSNP:770150730
2028 2028 a, g dbSNP:773095431
2041 2041 a, g dbSNP:559634238
2059 2059 a, t dbSNP:4251549
2093 2093 c, t dbSNP:191617315
2119 2119 a, t dbSNP:183559035
2151 2151 a, t dbSNP:4251550
2153 2153 a, c dbSNP:548838962
2239 2239 a, g dbSNP:4251551
2251 2251 a, g dbSNP:138178820
2254 2254 a, g dbSNP:4251552
2269 2269 a, c dbSNP:571568921
2297 2297 g, t dbSNP:774327325
2301 2301 g, t dbSNP:759513055
2312 2312 a, g dbSNP:538982204
2399 2399 c, t dbSNP:576622168
2449 2449 a, t dbSNP:767298091
2471 2471 a, c dbSNP:752547785
2480 2480 -, a dbSNP:36085436
2485 2485 a, g dbSNP:567630494
2507 2507 a, g dbSNP:566365168
2587 2587 a, g dbSNP:575385169
2598 2598 a, g dbSNP:542787354
2620 2620 g, t dbSNP:4251584
2698 2698 a, g dbSNP:574043929
2707 2707 c, t dbSNP:541034481
2727 2727 a, c dbSNP:559273571
2733 2733 a, g dbSNP:753237737
2738 2738 a, c dbSNP:777619096
2739 2739 a, g dbSNP:141847763
2792 2792 a, g dbSNP:545334973
2801 2801 c, t dbSNP:563991845
2818 2818 c, t dbSNP:755598323
2829 2829 a, c dbSNP:531038940
2842 2842 a, g dbSNP:781639576
2903 2903 g, t dbSNP:182497864
2904 2904 c, t dbSNP:111571285
2920 2920 a, c dbSNP:150604439
2927 2927 c, t dbSNP:528147840
2933 2933 a, c dbSNP:554628455
2937 2937 c, t dbSNP:770097373
2956 2956 a, g dbSNP:139778399
2960 2960 a, g dbSNP:563645022
2970 2970 -, ataa dbSNP:138875923
2971 2971 -, ataa dbSNP:527750692
2974 2974 -, ataa dbSNP:531640584
2989 2989 -, aaat dbSNP:368676868
2995 2995 g, t dbSNP:186825265
3017 3017 a, g dbSNP:530704544
3032 3032 g, t dbSNP:4251553
3146 3146 c, t dbSNP:536073958
3155 3155 c, t dbSNP:554707596
3156 3156 a, g dbSNP:542838156
3165 3165 a, g dbSNP:574051595
3174 3174 a, c, g dbSNP:139675032
3219 3219 a, g dbSNP:778083287
3238 3238 a, t dbSNP:553037446
3299 3299 c, g dbSNP:576135948
3313 3313 g, t dbSNP:192534409
3319 3319 -, g dbSNP:778897449
3323 3323 c, t dbSNP:544979835
3327 3327 a, g dbSNP:543426658
3350 3350 a, g dbSNP:771014920
3384 3384 c, t dbSNP:558662153
3391 3391 a, g dbSNP:144535012
3408 3408 a, t dbSNP:4251554
3425 3425 c, t dbSNP:4251555
3455 3455 -, ctt dbSNP:79794889
3456 3456 -, t dbSNP:538947924
3507 3507 a, g dbSNP:528687727
3514 3514 a, g dbSNP:546754318
3518 3518 c, t dbSNP:4251556
3519 3519 a, g dbSNP:183931852
3524 3524 c, t dbSNP:4251557
3526 3526 a, g dbSNP:4251558
3564 3564 a, c dbSNP:536497843
3572 3572 a, c dbSNP:548182708
3605 3605 a, g dbSNP:12321678
3645 3645 a, g dbSNP:533723416
3671 3671 a, g dbSNP:371821781
3692 3692 c, g dbSNP:533412197
3702 3702 c, t dbSNP:776314485
3711 3711 c, t dbSNP:776764561
3714 3714 c, g dbSNP:111813306
3731 3731 a, g dbSNP:534645871
3754 3754 g, t dbSNP:374721629
3819 3819 a, g dbSNP:1141168
3829 3829 c, t dbSNP:563217241
3854 3854 g, t dbSNP:769976979
3886 3886 g, t dbSNP:557276770
3897 3897 a, g dbSNP:4251560
3932 3932 g, t dbSNP:543346753
3976 3976 a, g dbSNP:368152755
3980 3980 c, t dbSNP:749964168
3989 3989 g, t dbSNP:554949427
4038 4038 -, tgtttaga dbSNP:747864158
4064 4064 c, t dbSNP:9849
4175 4175 a, g dbSNP:76405665
4193 4193 c, g, t dbSNP:145856072
4194 4194 a, g dbSNP:756483703
4196 4196 a, g dbSNP:549897461
4208 4208 g, t dbSNP:549623720
4243 4243 c, t dbSNP:147831464
4296 4296 c, t dbSNP:532207331
4297 4297 a, g, t dbSNP:375914722
4300 4300 c, t dbSNP:12310328
4333 4333 a, g dbSNP:749524568
4335 4335 c, t dbSNP:4251562
4356 4356 c, g dbSNP:766712571
4383 4383 c, t dbSNP:369823166
4384 4384 a, g dbSNP:562549392
4392 4392 a, g dbSNP:4251563
4435 4435 c, t dbSNP:771957948
4448 4448 a, g dbSNP:4251564

Target ORF information:

RefSeq Version XM_011538433
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011538433

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAACAAAC CCATAACACC ATCAACATAT GTGCGCTGCC TCAATGTTGG ACTAATTAGG 
AAGCTGTCAG ATTTTATTGA TCCTCAAGAA GGATGGAAGA AGTTAGCTGT AGCTATTAAA
AAACCATCTG GTGATGATAG ATACAATCAG TTTCACATAA GGAGATTTGA AGCATTACTT
CAAACTGGAA AAAGTCCCAC TTCTGAATTA CTGTTTGACT GGGGCACCAC AAATTGCACA
GTTGGTGATC TTGTGGATCT TTTGATCCAA AATGAATTTT TTGCTCCTGC GAGTCTTTTG
CTCCCAGATG CTGTTCCCAA AACTGCTAAT ACACTACCTT CTAAAGAAGC TATAACAGTT
CAGCAAAAAC AGATGCCTTT CTGTGACAAA GACAGGACAT TGATGACACC TGTGCAGAAT
CTTGAACAAA GCTATATGCC ACCTGACTCC TCAAGTCCAG AAAATAAAAG TTTAGAAGTT
AGTGATACAC GTTTTCACAG TTTTTCATTT TATGAATTGA AGAATGTCAC AAATAACTTT
GATGAACGAC CCATTTCTGT TGGTGGTAAT AAAATGGGAG AGGGAGGATT TGGAGTTGTA
TATAAAGGCT ACGTAAATAA CACAACTGTG GCAGTGAAGA AGCTTGCAGC AATGGTTGAC
ATTACTACTG AAGAACTGAA ACAGCAGTTT GATCAAGAAA TAAAAGTAAT GGCAAAGTGT
CAACATGAAA ACTTAGTAGA ACTACTTGGT TTCTCAAGTG ATGGAGATGA CCTCTGCTTA
GTATATGTTT ACATGCCTAA TGGTTCATTG CTAGACAGAC TCTCTTGCTT GGATGGTACT
CCACCACTTT CTTGGCACAT GAGATGCAAG ATTGCTCAGG GTGCAGCTAA TGGCATCAAT
TTTCTACATG AAAATCATCA TATTCATAGA GATATTAAAA GTGCAAATAT CTTACTGGAT
GAAGCTTTTA CTGCTAAAAT ATCTGACTTT GGCCTTGCAC GGGCTTCTGA GAAGTTTGCC
CAGACAGTCA TGACTAGCAG AATTGTGGGA ACAACAGCTT ATATGGCACC AGAAGCTTTG
CGTGGAGAAA TAACACCCAA ATCTGATATT TACAGCTTTG GTGTGGTTTT ACTAGAAATA
ATAACTGGAC TTCCAGCTGT GGATGAACAC CGTGAACCTC AGTTATTGCT AGATATTAAA
GAAGAAATTG AAGATGAAGA AAAGACAATT GAAGATTATA TTGATAAAAA GATGAATGAT
GCTGATTCCA CTTCAGTTGA AGCTATGTAC TCTGTTGCTA GTCAATGTCT GCATGAAAAG
AAAAATAAGA GACCAGACAT TAAGAAGGTT CAACAGCTGC TGCAAGAGAT GACAGCTTCT
TAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu15137D
Sequence Information ORF Nucleotide Sequence (Length: 1383bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product interleukin-1 receptor-associated kinase 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578823835. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAACAAAC CCATAACACC ATCAACATAT GTGCGCTGCC TCAATGTTGG ACTAATTAGG 
AAGCTGTCAG ATTTTATTGA TCCTCAAGAA GGATGGAAGA AGTTAGCTGT AGCTATTAAA
AAACCATCTG GTGATGATAG ATACAATCAG TTTCACATAA GGAGATTTGA AGCATTACTT
CAAACTGGAA AAAGTCCCAC TTCTGAATTA CTGTTTGACT GGGGCACCAC AAATTGCACA
GTTGGTGATC TTGTGGATCT TTTGATCCAA AATGAATTTT TTGCTCCTGC GAGTCTTTTG
CTCCCAGATG CTGTTCCCAA AACTGCTAAT ACACTACCTT CTAAAGAAGC TATAACAGTT
CAGCAAAAAC AGATGCCTTT CTGTGACAAA GACAGGACAT TGATGACACC TGTGCAGAAT
CTTGAACAAA GCTATATGCC ACCTGACTCC TCAAGTCCAG AAAATAAAAG TTTAGAAGTT
AGTGATACAC GTTTTCACAG TTTTTCATTT TATGAATTGA AGAATGTCAC AAATAACTTT
GATGAACGAC CCATTTCTGT TGGTGGTAAT AAAATGGGAG AGGGAGGATT TGGAGTTGTA
TATAAAGGCT ACGTAAATAA CACAACTGTG GCAGTGAAGA AGCTTGCAGC AATGGTTGAC
ATTACTACTG AAGAACTGAA ACAGCAGTTT GATCAAGAAA TAAAAGTAAT GGCAAAGTGT
CAACATGAAA ACTTAGTAGA ACTACTTGGT TTCTCAAGTG ATGGAGATGA CCTCTGCTTA
GTATATGTTT ACATGCCTAA TGGTTCATTG CTAGACAGAC TCTCTTGCTT GGATGGTACT
CCACCACTTT CTTGGCACAT GAGATGCAAG ATTGCTCAGG GTGCAGCTAA TGGCATCAAT
TTTCTACATG AAAATCATCA TATTCATAGA GATATTAAAA GTGCAAATAT CTTACTGGAT
GAAGCTTTTA CTGCTAAAAT ATCTGACTTT GGCCTTGCAC GGGCTTCTGA GAAGTTTGCC
CAGACAGTCA TGACTAGCAG AATTGTGGGA ACAACAGCTT ATATGGCACC AGAAGCTTTG
CGTGGAGAAA TAACACCCAA ATCTGATATT TACAGCTTTG GTGTGGTTTT ACTAGAAATA
ATAACTGGAC TTCCAGCTGT GGATGAACAC CGTGAACCTC AGTTATTGCT AGATATTAAA
GAAGAAATTG AAGATGAAGA AAAGACAATT GAAGATTATA TTGATAAAAA GATGAATGAT
GCTGATTCCA CTTCAGTTGA AGCTATGTAC TCTGTTGCTA GTCAATGTCT GCATGAAAAG
AAAAATAAGA GACCAGACAT TAAGAAGGTT CAACAGCTGC TGCAAGAGAT GACAGCTTCT
TAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005269000.1
CDS188..1570
Misc Feature(1)212..505(+)
Misc Feature(2)221..421(+)
Misc Feature(3)260..481(+)
Misc Feature(4)695..1558(+)
Misc Feature(5)752..1549(+)
Misc Feature(6)761..1246(+)
Misc Feature(7)761..1174(+)
Misc Feature(8)773..1246(+)
Misc Feature(9)1169..1246(+)
Translation
Position Chain Variation Link
37 37 c, g dbSNP:770034521
48 48 a, c dbSNP:189919547
57 57 a, g dbSNP:773753761
61 61 c, t dbSNP:530898596
83 83 g, t dbSNP:549501045
99 99 a, g dbSNP:567654648
113 113 c, t dbSNP:528426421
175 175 -, t dbSNP:765330474
192 192 a, g dbSNP:561048547
194 194 a, c dbSNP:752890848
198 198 c, t dbSNP:750912907
200 200 a, g dbSNP:56312115
203 203 a, c dbSNP:780540482
208 208 a, g dbSNP:201400143
214 214 a, c dbSNP:749572231
221 221 c, t dbSNP:377584435
222 222 a, g dbSNP:370524518
227 227 c, t dbSNP:748441973
231 231 a, g dbSNP:772274120
237 237 a, g dbSNP:778057572
241 241 a, g dbSNP:747100232
245 245 a, t dbSNP:143625818
250 250 a, g dbSNP:775601867
253 253 a, g dbSNP:762610309
263 263 a, g dbSNP:768548402
264 264 c, t dbSNP:138116867
269 269 a, c dbSNP:761596704
286 286 a, g dbSNP:115877973
299 299 a, c, g dbSNP:565544986
302 302 a, g dbSNP:113588409
304 304 a, t dbSNP:141039652
310 310 a, g dbSNP:551138584
318 318 g, t dbSNP:755265254
331 331 a, c dbSNP:779400593
336 336 a, t dbSNP:752850989
341 341 c, g dbSNP:758634981
349 349 a, g dbSNP:772625246
370 370 a, c dbSNP:778094168
372 372 c, g dbSNP:751881820
375 375 c, g dbSNP:572719034
393 393 a, c dbSNP:202134282
406 406 c, g, t dbSNP:374971450
410 410 a, g dbSNP:778743146
414 414 c, t dbSNP:200175187
417 417 a, c dbSNP:771900851
418 418 a, c dbSNP:772817746
431 431 a, g dbSNP:746729422
435 435 a, g dbSNP:770333429
439 439 c, t dbSNP:531518356
451 451 a, g dbSNP:776148970
453 453 c, t dbSNP:758974509
455 455 c, t dbSNP:765736285
460 460 c, t dbSNP:775885677
474 474 c, t dbSNP:763390934
477 477 c, t dbSNP:764597369
478 478 a, g dbSNP:17851028
481 481 g, t dbSNP:4251469
484 484 g, t dbSNP:757615268
490 490 c, t dbSNP:371634312
495 495 a, c, g dbSNP:774802371
501 501 -, tt dbSNP:763078267
505 505 c, g dbSNP:56338336
513 513 c, g dbSNP:750658320
520 520 -, a dbSNP:766515269
521 521 c, t dbSNP:756185480
530 530 a, g dbSNP:766433748
532 532 a, g dbSNP:752616965
533 533 a, g dbSNP:62642475
539 539 a, g dbSNP:540605517
558 558 a, c dbSNP:771105240
570 570 g, t dbSNP:777691189
576 576 a, g dbSNP:751316240
579 579 a, c dbSNP:757025762
586 586 a, g dbSNP:780877191
590 590 a, g dbSNP:745549223
593 593 a, g dbSNP:769345767
594 594 c, t dbSNP:779771826
601 601 a, g dbSNP:748825445
602 602 c, t dbSNP:769242383
610 610 c, t dbSNP:775016898
611 611 c, g dbSNP:762213099
612 612 a, t dbSNP:772359426
614 614 a, c dbSNP:773476797
618 618 c, g dbSNP:147079926
619 619 c, t dbSNP:766601065
624 624 g, t dbSNP:753847808
632 632 c, g dbSNP:375927287
633 633 a, g dbSNP:112228761
634 634 -, ctc dbSNP:556809469
640 640 -, a dbSNP:760054904
643 643 c, t dbSNP:763971241
649 649 a, t dbSNP:751549092
651 651 a, c dbSNP:757047226
654 654 a, g dbSNP:780919244
656 656 a, t dbSNP:750080879
657 657 g, t dbSNP:755668007
661 661 a, g dbSNP:779861617
663 663 a, t dbSNP:748754154
666 666 a, t dbSNP:201782959
671 671 a, g dbSNP:779528103
676 676 a, g, t dbSNP:748667787
692 692 c, t dbSNP:778367357
693 693 c, t dbSNP:747296110
709 709 g, t dbSNP:771324378
715 715 a, c dbSNP:776810085
716 716 a, g dbSNP:141209982
721 721 c, t dbSNP:769857009
724 724 c, g dbSNP:373731553
725 725 -, t dbSNP:753106997
734 734 c, t dbSNP:114951157
735 735 a, g dbSNP:186194251
748 748 c, t dbSNP:137949295
779 779 c, g dbSNP:773245379
779 779 -, g dbSNP:756802570
781 781 a, t dbSNP:113948349
785 785 c, g dbSNP:377074100
792 792 a, g dbSNP:191097365
799 799 c, t dbSNP:753538997
800 800 a, g dbSNP:370225662
825 825 a, t dbSNP:149453390
846 846 a, g dbSNP:781671136
855 855 c, t dbSNP:750733250
857 857 a, g dbSNP:201500711
865 865 a, g dbSNP:780121221
878 878 a, g dbSNP:111935971
884 884 a, g dbSNP:200082169
889 889 a, g, t dbSNP:778986995
892 892 a, t dbSNP:748253425
899 899 g, t dbSNP:770943951
907 907 c, t dbSNP:144854858
918 918 a, g dbSNP:781252640
929 929 c, t dbSNP:375755560
936 936 a, g dbSNP:769470855
945 945 c, g dbSNP:775286298
947 947 g, t dbSNP:762667477
948 948 a, t dbSNP:768303231
950 950 a, g dbSNP:774020314
953 953 a, g dbSNP:761277223
958 958 c, t dbSNP:767075679
972 972 a, g dbSNP:773708174
973 973 c, t dbSNP:201704200
974 974 a, g dbSNP:112517169
978 978 a, t dbSNP:766812248
994 994 a, c dbSNP:754184412
1006 1006 a, t dbSNP:370042548
1009 1009 c, g, t dbSNP:755330743
1012 1012 c, t dbSNP:140210540
1019 1019 a, g dbSNP:757311009
1020 1020 a, g, t dbSNP:780005506
1021 1021 c, t dbSNP:754767022
1037 1037 c, t dbSNP:778616067
1042 1042 g, t dbSNP:747953616
1047 1047 g, t dbSNP:771667566
1052 1052 c, t dbSNP:373167326
1057 1057 c, g dbSNP:145622820
1061 1061 c, g dbSNP:770396929
1064 1064 c, t dbSNP:121908002
1065 1065 a, g dbSNP:760186666
1067 1067 a, g dbSNP:200883675
1068 1068 a, g dbSNP:775937250
1076 1076 a, g dbSNP:556565376
1080 1080 a, g, t dbSNP:568782766
1086 1086 a, g dbSNP:376948391
1087 1087 c, t dbSNP:536101269
1093 1093 a, t dbSNP:750525615
1103 1103 a, c dbSNP:115573494
1104 1104 a, t dbSNP:778846957
1110 1110 a, t dbSNP:752451445
1111 1111 a, t dbSNP:374532899
1122 1122 c, t dbSNP:777460833
1130 1130 a, g dbSNP:751373844
1137 1137 a, t dbSNP:756802579
1148 1148 a, g dbSNP:780917972
1149 1149 a, t dbSNP:147301051
1152 1152 c, g dbSNP:769465559
1160 1160 a, g dbSNP:780681951
1177 1177 c, t dbSNP:749864140
1179 1179 g, t dbSNP:769214804
1187 1187 c, t dbSNP:774525787
1188 1188 a, g dbSNP:748570560
1205 1205 g, t dbSNP:772148472
1216 1216 c, t dbSNP:773464920
1224 1224 a, g dbSNP:368108618
1244 1244 a, g dbSNP:140869783
1250 1250 a, c, g dbSNP:142376871
1260 1260 a, t dbSNP:775402267
1268 1268 c, t dbSNP:762731086
1269 1269 a, g dbSNP:764106350
1271 1271 a, g dbSNP:751261930
1285 1285 c, t dbSNP:757104944
1296 1296 c, t dbSNP:372448559
1298 1298 g, t dbSNP:750146218
1307 1307 a, g dbSNP:779468411
1310 1310 a, g dbSNP:755668302
1311 1311 c, t dbSNP:779773825
1329 1329 c, t dbSNP:767476422
1332 1332 c, g dbSNP:750092963
1333 1333 -, tg dbSNP:764404378
1335 1335 a, g, t dbSNP:755772831
1336 1336 a, g dbSNP:753251856
1348 1348 a, g dbSNP:757918302
1350 1350 a, t dbSNP:151272504
1356 1356 a, g dbSNP:4251583
1358 1358 c, t dbSNP:114820168
1359 1359 a, g dbSNP:55944915
1364 1364 c, g dbSNP:758688655
1378 1378 a, g dbSNP:773095217
1386 1386 -, aag dbSNP:758148548
1388 1388 a, g dbSNP:760580944
1391 1391 g, t dbSNP:766102187
1409 1409 a, c dbSNP:547810966
1412 1412 a, g dbSNP:758956283
1415 1415 a, g dbSNP:764868568
1416 1416 c, t dbSNP:146538989
1427 1427 a, g dbSNP:758846627
1428 1428 c, t dbSNP:778274322
1448 1448 a, g dbSNP:751879000
1466 1466 a, g dbSNP:757657887
1469 1469 a, g dbSNP:4251545
1473 1473 c, t dbSNP:533369384
1478 1478 g, t dbSNP:769754150
1480 1480 g, t dbSNP:780112532
1481 1481 c, g dbSNP:749338213
1484 1484 a, g dbSNP:772098333
1485 1485 c, g dbSNP:773326632
1488 1488 c, g dbSNP:760527974
1489 1489 c, t dbSNP:551524673
1493 1493 a, t dbSNP:770908857
1494 1494 c, g dbSNP:776143317
1498 1498 a, g dbSNP:759164006
1501 1501 c, t dbSNP:144842940
1516 1516 c, g dbSNP:752201648
1518 1518 g, t dbSNP:780633823
1520 1520 a, c, t dbSNP:762479548
1521 1521 a, c dbSNP:752108446
1525 1525 c, t dbSNP:138719119
1527 1527 a, g, t dbSNP:569421834
1531 1531 g, t dbSNP:756298129
1538 1538 c, g dbSNP:762236455
1552 1552 a, g dbSNP:767921421
1553 1553 a, g dbSNP:148873968
1554 1554 a, t dbSNP:372068514
1558 1558 a, g dbSNP:766606181
1560 1560 c, t dbSNP:754175088
1574 1574 c, t dbSNP:755104443
1577 1577 a, g dbSNP:567188868
1587 1587 a, t dbSNP:748253330
1589 1589 c, t dbSNP:199727839
1594 1594 a, g dbSNP:781021520
1597 1597 c, t dbSNP:781731425
1603 1603 a, g dbSNP:745643347
1611 1611 a, g dbSNP:553330681
1613 1613 c, g dbSNP:769526114
1621 1621 -, t dbSNP:755909125
1622 1622 a, t dbSNP:184380424
1682 1682 a, g dbSNP:577974384
1716 1716 c, t dbSNP:4251546
1720 1720 a, g dbSNP:182322900
1815 1815 a, g dbSNP:187201106
1840 1840 c, t dbSNP:4251547
1851 1851 a, g dbSNP:4251548
1875 1875 g, t dbSNP:115845154
1889 1889 a, g dbSNP:769535835
1912 1912 g, t dbSNP:147004395
1913 1913 a, g dbSNP:770150730
1967 1967 a, g dbSNP:773095431
1980 1980 a, g dbSNP:559634238
1998 1998 a, t dbSNP:4251549
2032 2032 c, t dbSNP:191617315
2058 2058 a, t dbSNP:183559035
2090 2090 a, t dbSNP:4251550
2092 2092 a, c dbSNP:548838962
2178 2178 a, g dbSNP:4251551
2190 2190 a, g dbSNP:138178820
2193 2193 a, g dbSNP:4251552
2208 2208 a, c dbSNP:571568921
2236 2236 g, t dbSNP:774327325
2240 2240 g, t dbSNP:759513055
2251 2251 a, g dbSNP:538982204
2338 2338 c, t dbSNP:576622168
2388 2388 a, t dbSNP:767298091
2410 2410 a, c dbSNP:752547785
2419 2419 -, a dbSNP:36085436
2424 2424 a, g dbSNP:567630494
2446 2446 a, g dbSNP:566365168
2526 2526 a, g dbSNP:575385169
2537 2537 a, g dbSNP:542787354
2559 2559 g, t dbSNP:4251584
2637 2637 a, g dbSNP:574043929
2646 2646 c, t dbSNP:541034481
2666 2666 a, c dbSNP:559273571
2672 2672 a, g dbSNP:753237737
2677 2677 a, c dbSNP:777619096
2678 2678 a, g dbSNP:141847763
2731 2731 a, g dbSNP:545334973
2740 2740 c, t dbSNP:563991845
2757 2757 c, t dbSNP:755598323
2768 2768 a, c dbSNP:531038940
2781 2781 a, g dbSNP:781639576
2842 2842 g, t dbSNP:182497864
2843 2843 c, t dbSNP:111571285
2859 2859 a, c dbSNP:150604439
2866 2866 c, t dbSNP:528147840
2872 2872 a, c dbSNP:554628455
2876 2876 c, t dbSNP:770097373
2895 2895 a, g dbSNP:139778399
2899 2899 a, g dbSNP:563645022
2909 2909 -, ataa dbSNP:138875923
2910 2910 -, ataa dbSNP:527750692
2913 2913 -, ataa dbSNP:531640584
2928 2928 -, aaat dbSNP:368676868
2934 2934 g, t dbSNP:186825265
2956 2956 a, g dbSNP:530704544
2971 2971 g, t dbSNP:4251553
3085 3085 c, t dbSNP:536073958
3094 3094 c, t dbSNP:554707596
3095 3095 a, g dbSNP:542838156
3104 3104 a, g dbSNP:574051595
3113 3113 a, c, g dbSNP:139675032
3158 3158 a, g dbSNP:778083287
3177 3177 a, t dbSNP:553037446
3238 3238 c, g dbSNP:576135948
3252 3252 g, t dbSNP:192534409
3258 3258 -, g dbSNP:778897449
3262 3262 c, t dbSNP:544979835
3266 3266 a, g dbSNP:543426658
3289 3289 a, g dbSNP:771014920
3323 3323 c, t dbSNP:558662153
3330 3330 a, g dbSNP:144535012
3347 3347 a, t dbSNP:4251554
3364 3364 c, t dbSNP:4251555
3394 3394 -, ctt dbSNP:79794889
3395 3395 -, t dbSNP:538947924
3446 3446 a, g dbSNP:528687727
3453 3453 a, g dbSNP:546754318
3457 3457 c, t dbSNP:4251556
3458 3458 a, g dbSNP:183931852
3463 3463 c, t dbSNP:4251557
3465 3465 a, g dbSNP:4251558
3503 3503 a, c dbSNP:536497843
3511 3511 a, c dbSNP:548182708
3544 3544 a, g dbSNP:12321678
3584 3584 a, g dbSNP:533723416
3610 3610 a, g dbSNP:371821781
3631 3631 c, g dbSNP:533412197
3641 3641 c, t dbSNP:776314485
3650 3650 c, t dbSNP:776764561
3653 3653 c, g dbSNP:111813306
3670 3670 a, g dbSNP:534645871
3693 3693 g, t dbSNP:374721629
3758 3758 a, g dbSNP:1141168
3768 3768 c, t dbSNP:563217241
3793 3793 g, t dbSNP:769976979
3825 3825 g, t dbSNP:557276770
3836 3836 a, g dbSNP:4251560
3871 3871 g, t dbSNP:543346753
3915 3915 a, g dbSNP:368152755
3919 3919 c, t dbSNP:749964168
3928 3928 g, t dbSNP:554949427
3977 3977 -, tgtttaga dbSNP:747864158
4003 4003 c, t dbSNP:9849
4114 4114 a, g dbSNP:76405665
4132 4132 c, g, t dbSNP:145856072
4133 4133 a, g dbSNP:756483703
4135 4135 a, g dbSNP:549897461
4147 4147 g, t dbSNP:549623720
4182 4182 c, t dbSNP:147831464
4235 4235 c, t dbSNP:532207331
4236 4236 a, g, t dbSNP:375914722
4239 4239 c, t dbSNP:12310328
4272 4272 a, g dbSNP:749524568
4274 4274 c, t dbSNP:4251562
4295 4295 c, g dbSNP:766712571
4322 4322 c, t dbSNP:369823166
4323 4323 a, g dbSNP:562549392
4331 4331 a, g dbSNP:4251563
4374 4374 c, t dbSNP:771957948
4387 4387 a, g dbSNP:4251564

Target ORF information:

RefSeq Version XM_005268943
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005268943

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAACAAAC CCATAACACC ATCAACATAT GTGCGCTGCC TCAATGTTGG ACTAATTAGG 
AAGCTGTCAG ATTTTATTGA TCCTCAAGAA GGATGGAAGA AGTTAGCTGT AGCTATTAAA
AAACCATCTG GTGATGATAG ATACAATCAG TTTCACATAA GGAGATTTGA AGCATTACTT
CAAACTGGAA AAAGTCCCAC TTCTGAATTA CTGTTTGACT GGGGCACCAC AAATTGCACA
GTTGGTGATC TTGTGGATCT TTTGATCCAA AATGAATTTT TTGCTCCTGC GAGTCTTTTG
CTCCCAGATG CTGTTCCCAA AACTGCTAAT ACACTACCTT CTAAAGAAGC TATAACAGTT
CAGCAAAAAC AGATGCCTTT CTGTGACAAA GACAGGACAT TGATGACACC TGTGCAGAAT
CTTGAACAAA GCTATATGCC ACCTGACTCC TCAAGTCCAG AAAATAAAAG TTTAGAAGTT
AGTGATACAC GTTTTCACAG TTTTTCATTT TATGAATTGA AGAATGTCAC AAATAACTTT
GATGAACGAC CCATTTCTGT TGGTGGTAAT AAAATGGGAG AGGGAGGATT TGGAGTTGTA
TATAAAGGCT ACGTAAATAA CACAACTGTG GCAGTGAAGA AGCTTGCAGC AATGGTTGAC
ATTACTACTG AAGAACTGAA ACAGCAGTTT GATCAAGAAA TAAAAGTAAT GGCAAAGTGT
CAACATGAAA ACTTAGTAGA ACTACTTGGT TTCTCAAGTG ATGGAGATGA CCTCTGCTTA
GTATATGTTT ACATGCCTAA TGGTTCATTG CTAGACAGAC TCTCTTGCTT GGATGGTACT
CCACCACTTT CTTGGCACAT GAGATGCAAG ATTGCTCAGG GTGCAGCTAA TGGCATCAAT
TTTCTACATG AAAATCATCA TATTCATAGA GATATTAAAA GTGCAAATAT CTTACTGGAT
GAAGCTTTTA CTGCTAAAAT ATCTGACTTT GGCCTTGCAC GGGCTTCTGA GAAGTTTGCC
CAGACAGTCA TGACTAGCAG AATTGTGGGA ACAACAGCTT ATATGGCACC AGAAGCTTTG
CGTGGAGAAA TAACACCCAA ATCTGATATT TACAGCTTTG GTGTGGTTTT ACTAGAAATA
ATAACTGGAC TTCCAGCTGT GGATGAACAC CGTGAACCTC AGTTATTGCT AGATATTAAA
GAAGAAATTG AAGATGAAGA AAAGACAATT GAAGATTATA TTGATAAAAA GATGAATGAT
GCTGATTCCA CTTCAGTTGA AGCTATGTAC TCTGTTGCTA GTCAATGTCT GCATGAAAAG
AAAAATAAGA GACCAGACAT TAAGAAGGTT CAACAGCTGC TGCAAGAGAT GACAGCTTCT
TAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu15137D
Sequence Information ORF Nucleotide Sequence (Length: 1383bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product interleukin-1 receptor-associated kinase 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578823833. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAACAAAC CCATAACACC ATCAACATAT GTGCGCTGCC TCAATGTTGG ACTAATTAGG 
AAGCTGTCAG ATTTTATTGA TCCTCAAGAA GGATGGAAGA AGTTAGCTGT AGCTATTAAA
AAACCATCTG GTGATGATAG ATACAATCAG TTTCACATAA GGAGATTTGA AGCATTACTT
CAAACTGGAA AAAGTCCCAC TTCTGAATTA CTGTTTGACT GGGGCACCAC AAATTGCACA
GTTGGTGATC TTGTGGATCT TTTGATCCAA AATGAATTTT TTGCTCCTGC GAGTCTTTTG
CTCCCAGATG CTGTTCCCAA AACTGCTAAT ACACTACCTT CTAAAGAAGC TATAACAGTT
CAGCAAAAAC AGATGCCTTT CTGTGACAAA GACAGGACAT TGATGACACC TGTGCAGAAT
CTTGAACAAA GCTATATGCC ACCTGACTCC TCAAGTCCAG AAAATAAAAG TTTAGAAGTT
AGTGATACAC GTTTTCACAG TTTTTCATTT TATGAATTGA AGAATGTCAC AAATAACTTT
GATGAACGAC CCATTTCTGT TGGTGGTAAT AAAATGGGAG AGGGAGGATT TGGAGTTGTA
TATAAAGGCT ACGTAAATAA CACAACTGTG GCAGTGAAGA AGCTTGCAGC AATGGTTGAC
ATTACTACTG AAGAACTGAA ACAGCAGTTT GATCAAGAAA TAAAAGTAAT GGCAAAGTGT
CAACATGAAA ACTTAGTAGA ACTACTTGGT TTCTCAAGTG ATGGAGATGA CCTCTGCTTA
GTATATGTTT ACATGCCTAA TGGTTCATTG CTAGACAGAC TCTCTTGCTT GGATGGTACT
CCACCACTTT CTTGGCACAT GAGATGCAAG ATTGCTCAGG GTGCAGCTAA TGGCATCAAT
TTTCTACATG AAAATCATCA TATTCATAGA GATATTAAAA GTGCAAATAT CTTACTGGAT
GAAGCTTTTA CTGCTAAAAT ATCTGACTTT GGCCTTGCAC GGGCTTCTGA GAAGTTTGCC
CAGACAGTCA TGACTAGCAG AATTGTGGGA ACAACAGCTT ATATGGCACC AGAAGCTTTG
CGTGGAGAAA TAACACCCAA ATCTGATATT TACAGCTTTG GTGTGGTTTT ACTAGAAATA
ATAACTGGAC TTCCAGCTGT GGATGAACAC CGTGAACCTC AGTTATTGCT AGATATTAAA
GAAGAAATTG AAGATGAAGA AAAGACAATT GAAGATTATA TTGATAAAAA GATGAATGAT
GCTGATTCCA CTTCAGTTGA AGCTATGTAC TCTGTTGCTA GTCAATGTCT GCATGAAAAG
AAAAATAAGA GACCAGACAT TAAGAAGGTT CAACAGCTGC TGCAAGAGAT GACAGCTTCT
TAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005269002.1
CDS395..1777
Misc Feature(1)419..712(+)
Misc Feature(2)428..628(+)
Misc Feature(3)467..688(+)
Misc Feature(4)902..1765(+)
Misc Feature(5)959..1756(+)
Misc Feature(6)968..1453(+)
Misc Feature(7)968..1381(+)
Misc Feature(8)980..1453(+)
Misc Feature(9)1376..1453(+)
Translation
Position Chain Variation Link
8 8 a, g dbSNP:546604862
20 20 a, g dbSNP:750677998
23 23 c, g dbSNP:763261637
30 30 a, t dbSNP:758663016
101 101 c, t dbSNP:571580877
103 103 a, c dbSNP:538974475
118 118 c, t dbSNP:751628808
163 163 a, g dbSNP:557115716
191 191 c, t dbSNP:4251567
224 224 a, c dbSNP:576881180
228 228 g, t dbSNP:554603533
241 241 c, t dbSNP:4251423
264 264 a, g dbSNP:572864111
332 332 c, t dbSNP:4251568
339 339 c, t dbSNP:547329673
372 372 a, c dbSNP:181596834
399 399 a, g dbSNP:561048547
401 401 a, c dbSNP:752890848
405 405 c, t dbSNP:750912907
407 407 a, g dbSNP:56312115
410 410 a, c dbSNP:780540482
415 415 a, g dbSNP:201400143
421 421 a, c dbSNP:749572231
428 428 c, t dbSNP:377584435
429 429 a, g dbSNP:370524518
434 434 c, t dbSNP:748441973
438 438 a, g dbSNP:772274120
444 444 a, g dbSNP:778057572
448 448 a, g dbSNP:747100232
452 452 a, t dbSNP:143625818
457 457 a, g dbSNP:775601867
460 460 a, g dbSNP:762610309
470 470 a, g dbSNP:768548402
471 471 c, t dbSNP:138116867
476 476 a, c dbSNP:761596704
493 493 a, g dbSNP:115877973
506 506 a, c, g dbSNP:565544986
509 509 a, g dbSNP:1135