×

GLRX5 cDNA ORF clone, Homo sapiens (human)

Gene Symbol GLRX5
Entrez Gene ID 51218
Full Name glutaredoxin 5
Synonyms C14orf87, FLB4739, GRX5, PR01238, PRO1238, PRSA
General protein information
Preferred Names
glutaredoxin-related protein 5, mitochondrial
Names
glutaredoxin-related protein 5, mitochondrial
glutaredoxin 5 homolog
monothiol glutaredoxin-5
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

14

14q32.13

Summary This gene encodes a mitochondrial protein, which is evolutionarily conserved. It is involved in the biogenesis of iron-sulfur clusters, which are required for normal iron homeostasis. Mutations in this gene are associated with autosomal recessive pyridoxine-refractory sideroblastic anemia. [provided by RefSeq, May 2010]. lac of sum
Disorder MIM:

609588

Disorder Html: Anemia, sideroblastic, pyridoxine-refractory, autosomal recessive,

mRNA and Protein(s)

mRNA Protein Name
NM_016417 NP_057501 glutaredoxin-related protein 5, mitochondrial precursor


Homo sapiens (human) GLRX5 NP_057501.2
Pan troglodytes (chimpanzee) GLRX5 XP_001154482.1
Macaca mulatta (Rhesus monkey) GLRX5 XP_001101070.1
Bos taurus (cattle) GLRX5 NP_001093773.1
Mus musculus (house mouse) Glrx5 NP_082695.1
Rattus norvegicus (Norway rat) Glrx5 NP_001102192.1
Gallus gallus (chicken) GLRX5 NP_001008472.1
Danio rerio (zebrafish) glrx5 NP_998186.1
Drosophila melanogaster (fruit fly) CG14407 NP_572974.1
Caenorhabditis elegans glrx-5 NP_499610.1
Xenopus (Silurana) tropicalis (western clawed frog) glrx5 NP_001004919.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GLRX5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLRX5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18481
NM_016417 Homo sapiens glutaredoxin 5 (GLRX5), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18481
Clone ID Related Accession (Same CDS sequence) NM_016417
Accession Version NM_016417.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 474bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glutaredoxin-related protein 5, mitochondrial precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI259789.1, BX248075.1, BC047680.1, BI834047.1 and AI089763.1. This sequence is a reference standard in the RefSeqGene project. On Feb 10, 2004 this sequence version replaced gi:9994192. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BI599990.1, BC047680.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
ATGAGCGGGT CCCTCGGCCG AGCTGCGGCG GCTCTGCTCC GCTGGGGGCG CGGCGCGGGC 
GGCGGTGGCC TTTGGGGTCC GGGCGTGCGG GCGGCGGGCT CGGGCGCGGG CGGCGGCGGC
TCGGCGGAGC AGTTGGACGC GCTGGTGAAG AAGGACAAGG TGGTGGTCTT CCTCAAGGGG
ACGCCGGAGC AGCCCCAGTG CGGCTTCAGC AACGCCGTGG TGCAGATCCT GCGGCTGCAC
GGCGTCCGCG ATTACGCGGC CTACAACGTG CTGGACGACC CGGAGCTCCG ACAAGGCATT
AAAGACTATT CCAACTGGCC CACCATCCCG CAAGTGTACC TCAATGGCGA GTTTGTAGGG
GGCTGTGACA TTCTTCTGCA GATGCACCAG AATGGGGACT TGGTGGAAGA ACTGAAAAAG
CTGGGGATCC ACTCCGCCCT TTTAGATGAA AAGAAAGACC AAGACTCCAA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_057501.2
CDS105..578
Misc Feature(1)3..5(+)
Misc Feature(2)237..509(+)
Misc Feature(3)279..431(+)
Misc Feature(4)279..281(+)
Misc Feature(5)303..314(+)
Misc Feature(6)393..407(+)
Misc Feature(7)468..473(+)
Exon (1)1..399
Gene:GLRX5
Gene Synonym:
Exon (2)400..1171
Gene:GLRX5
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_016417
Organism Homo sapiens (human)
Definition Homo sapiens glutaredoxin 5 (GLRX5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_016417

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGAGCGGGT CCCTCGGCCG AGCTGCGGCG GCTCTGCTCC GCTGGGGGCG CGGCGCGGGC 
GGCGGTGGCC TTTGGGGTCC GGGCGTGCGG GCGGCGGGCT CGGGCGCGGG CGGCGGCGGC
TCGGCGGAGC AGTTGGACGC GCTGGTGAAG AAGGACAAGG TGGTGGTCTT CCTCAAGGGG
ACGCCGGAGC AGCCCCAGTG CGGCTTCAGC AACGCCGTGG TGCAGATCCT GCGGCTGCAC
GGCGTCCGCG ATTACGCGGC CTACAACGTG CTGGACGACC CGGAGCTCCG ACAAGGCATT
AAAGACTATT CCAACTGGCC CACCATCCCG CAAGTGTACC TCAATGGCGA GTTTGTAGGG
GGCTGTGACA TTCTTCTGCA GATGCACCAG AATGGGGACT TGGTGGAAGA ACTGAAAAAG
CTGGGGATCC ACTCCGCCCT TTTAGATGAA AAGAAAGACC AAGACTCCAA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Genome-wide association study combining pathway analysis for typical sporadic amyotrophic lateral sclerosis in Chinese Han populations
Neurobiol. Aging 35 (7), 1778 (2014)
Xie T, Deng L, Mei P, Zhou Y, Wang B, Zhang J, Lin J, Wei Y, Zhang X and Xu R.


book

Variant non ketotic hyperglycinemia is caused by mutations in LIAS, BOLA3 and the novel gene GLRX5
Brain 137 (PT 2), 366-379 (2014)
Baker PR 2nd, Friederich MW, Swanson MA, Shaikh T, Bhattacharya K, Scharer GH, Aicher J, Creadon-Swindell G, Geiger E, MacLean KN, Lee WT, Deshpande C, Freckmann ML, Shih LY, Wasserstein M, Rasmussen MB, Lund AM, Procopis P, Cameron JM, Robinson BH, Brown GK, Brown RM, Compton AG, Dieckmann CL, Collard R, Coughlin CR 2nd, Spector E, Wempe MF and Van Hove JL.


book

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population
PLoS ONE 7 (12), E51954 (2012)
Comuzzie AG, Cole SA, Laston SL, Voruganti VS, Haack K, Gibbs RA and Butte NF.


book

The crystal structure of human GLRX5: iron-sulfur cluster co-ordination, tetrameric assembly and monomer activity
Biochem. J. 433 (2), 303-311 (2011)
Johansson C, Roos AK, Montano SJ, Sengupta R, Filippakopoulos P, Guo K, von Delft F, Holmgren A, Oppermann U and Kavanagh KL.


book

Glutaredoxin 5 deficiency causes sideroblastic anemia by specifically impairing heme biosynthesis and depleting cytosolic iron in human erythroblasts
J. Clin. Invest. 120 (5), 1749-1761 (2010)
Ye H, Jeong SY, Ghosh MC, Kovtunovych G, Silvestri L, Ortillo D, Uchida N, Tisdale J, Camaschella C and Rouault TA.


book

Systematic molecular genetic analysis of congenital sideroblastic anemia: evidence for genetic heterogeneity and identification of novel mutations
Pediatr Blood Cancer 54 (2), 273-278 (2010)
Bergmann AK, Campagna DR, McLoughlin EM, Agarwal S, Fleming MD, Bottomley SS and Neufeld EJ.


book

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression
PLoS ONE 5 (9), E12862 (2010)
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL and O'Brien SJ.


book

The human counterpart of zebrafish shiraz shows sideroblastic-like microcytic anemia and iron overload
Blood 110 (4), 1353-1358 (2007)
Camaschella C, Campanella A, De Falco L, Boschetto L, Merlini R, Silvestri L, Levi S and Iolascon A.


book

Deficiency of glutaredoxin 5 reveals Fe-S clusters are required for vertebrate haem synthesis
Nature 436 (7053), 1035-1039 (2005)
Wingert RA, Galloway JL, Barut B, Foott H, Fraenkel P, Axe JL, Weber GJ, Dooley K, Davidson AJ, Schmid B, Paw BH, Shaw GC, Kingsley P, Palis J, Schubert H, Chen O, Kaplan J and Zon LI.


 
*
*
*
*