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NME8 cDNA ORF clone, Homo sapiens (human)

Gene Symbol NME8
Entrez Gene ID 51314
Full Name NME/NM23 family member 8
Synonyms CILD6, NM23-H8, SPTRX2, TXNDC3, sptrx-2
General protein information
Preferred Names
thioredoxin domain-containing protein 3
Names
thioredoxin domain-containing protein 3
sperm-specific thioredoxin 2
spermatid-specific thioredoxin-2
thioredoxin domain containing 3 (spermatozoa)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7p14.1

Summary This gene encodes a protein with an N-terminal thioredoxin domain and three C-terminal nucleoside diphosphate kinase (NDK) domains, but the NDK domains are thought to be catalytically inactive. The sea urchin ortholog of this gene encodes a component of sperm outer dynein arms, and the protein is implicated in ciliary function. Mutations in this gene are implicated in primary ciliary dyskinesia type 6.[provided by RefSeq, Nov 2009]. lac of sum
Disorder MIM:

607421

Disorder Html: Ciliary dyskinesia, primary, 6, 610852 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_016616 NP_057700 thioredoxin domain-containing protein 3


Homo sapiens (human) NME8 NP_057700.3
Pan troglodytes (chimpanzee) TXNDC3 XP_527723.3
Macaca mulatta (Rhesus monkey) TXNDC3 XP_001101594.1
Canis lupus familiaris (dog) NME8 XP_851794.1
Mus musculus (house mouse) Nme8 NP_853622.2
Rattus norvegicus (Norway rat) Nme8 NP_942087.2
Gallus gallus (chicken) TXNDC3 XP_426021.3
Danio rerio (zebrafish) nme8 NP_001082944.1
Xenopus (Silurana) tropicalis (western clawed frog) nme9 NP_001121456.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NME8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NME8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu27090 NM_016616 Homo sapiens NME/NM23 family member 8 (NME8), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27090
Clone ID Related Accession (Same CDS sequence) NM_016616
Accession Version NM_016616.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1767bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product thioredoxin domain-containing protein 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC036816.1, AF202051.2 and AF305596.1. This sequence is a reference standard in the RefSeqGene project. On or before Aug 31, 2013 this sequence version replaced gi:530384802, gi:148839371. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151119, SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCAAGCA AAAAACGAGA AGTCCAGTTA CAGACAGTCA TCAATAATCA AAGCCTGTGG 
GATGAGATGT TGCAGAACAA AGGCTTAACA GTGATTGATG TTTACCAAGC CTGGTGTGGA
CCTTGCAGAG CAATGCAACC TTTATTCAGA AAATTGAAAA ATGAACTGAA CGAAGACGAA
ATTCTGCATT TTGCTGTCGC AGAAGCTGAC AACATTGTGA CTTTGCAGCC ATTTAGAGAT
AAATGTGAAC CTGTTTTTCT CTTTAGTGTT AATGGCAAAA TTATCGAAAA GATTCAGGGT
GCAAATGCAC CGCTTGTTAA TAAAAAAGTT ATTAATTTGA TCGATGAGGA GAGAAAAATT
GCAGCAGGTG AAATGGCTCG ACCTCAGTAT CCTGAAATTC CATTAGTAGA CTCAGATTCA
GAAGTTAGTG AAGAATCACC ATGTGAAAGT GTTCAGGAAT TATACAGTAT TGCTATTATC
AAACCGGATG CTGTGATTAG TAAAAAAGTT CTAGAAATTA AAAGAAAAAT TACCAAAGCT
GGATTTATTA TAGAAGCAGA GCATAAGACA GTGCTCACTG AAGAACAAGT TGTCAACTTC
TATAGTCGAA TAGCAGACCA GTGTGACTTC GAAGAGTTTG TCTCTTTTAT GACAAGTGGC
TTAAGCTATA TTCTAGTTGT ATCTCAAGGA AGTAAACACA ATCCTCCCTC TGAAGAAACC
GAACCACAGA CTGACACCGA ACCTAACGAA CGATCTGAGG ATCAACCTGA GGTCGAAGCC
CAGGTTACAC CTGGAATGAT GAAGAACAAA CAAGACAGTT TACAAGAATA TCTGGAAAGA
CAACATTTAG CTCAGCTCTG TGACATTGAA GAGGATGCAG CTAATGTTGC TAAGTTCATG
GATGCTTTCT TCCCCGATTT TAAAAAAATG AAAAGCATGA AATTAGAAAA GACATTGGCA
TTACTTCGAC CAAATCTCTT TCATGAAAGG AAAGATGATG TTTTGCGTAT TATTAAAGAT
GAAGACTTCA AAATACTGGA GCAAAGACAA GTAGTATTAT CGGAAAAAGA AGCACAAGCA
CTGTGCAAGG AATATGAAAA TGAAGACTAT TTTAATAAAC TTATAGAAAA CATGACCAGT
GGTCCATCTC TAGCCCTTGT TTTATTGAGA GACAATGGCT TGCAATACTG GAAACAATTA
CTGGGACCAA GAACTGTTGA AGAAGCCATT GAATATTTTC CAGAGAGTTT ATGTGCACAG
TTTGCGATGG ACAGTTTGCC GGTCAACCAG TTGTATGGCA GCGATTCATT AGAAACCGCT
GAAAGGGAAA TACAGCATTT CTTTCCTCTT CAAAGCACTT TAGGCTTGAT TAAACCTCAT
GCAACAAGTG AACAAAGAGA GCAGATCCTG AAGATAGTTA AGGAGGCTGG ATTTGATCTG
ACACAGGTGA AGAAAATGTT CCTAACTCCT GAGCAAATAG AGAAAATTTA TCCAAAAGTA
ACAGGAAAAG ACTTTTATAA AGATTTATTG GAAATGTTAT CTGTGGGTCC ATCTATGGTC
ATGATTCTGA CCAAGTGGAA TGCTGTTGCA GAATGGAGAC GATTGATGGG CCCAACAGAC
CCAGAAGAAG CAAAATTACT TTCCCCTGAC TCCATCCGAG CCCAGTTTGG AATAAGTAAA
TTGAAAAACA TTGTCCATGG AGCATCTAAC GCCTATGAAG CAAAAGAGGT TGTTAATAGA
CTCTTTGAGG ATCCTGAGGA AAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_057700.3
CDS373..2139
Misc Feature(1)370..372(+)
Misc Feature(2)403..711(+)
Misc Feature(3)487..498(+)
Misc Feature(4)832..1284(+)
Misc Feature(5)841..1143(+)
Misc Feature(6)853..1269(+)
Misc Feature(7)865..939(+)
Misc Feature(8)1315..1737(+)
Misc Feature(9)1318..1731(+)
Misc Feature(10)1318..1716(+)
Misc Feature(11)1339..1701(+)
Misc Feature(12)1351..1422(+)
Misc Feature(13)1723..2130(+)
Misc Feature(14)1723..2118(+)
Misc Feature(15)1738..2136(+)
Misc Feature(16)1744..2103(+)
Misc Feature(17)1756..1827(+)
Exon (1)1..132
Gene:NME8
Gene Synonym:
Exon (2)133..365
Gene:NME8
Gene Synonym:
Exon (3)366..405
Gene:NME8
Gene Synonym:
Exon (4)406..463
Gene:NME8
Gene Synonym:
Exon (5)464..570
Gene:NME8
Gene Synonym:
Exon (6)571..642
Gene:NME8
Gene Synonym:
Exon (7)643..759
Gene:NME8
Gene Synonym:
Exon (8)760..826
Gene:NME8
Gene Synonym:
Exon (9)827..900
Gene:NME8
Gene Synonym:
Exon (10)901..993
Gene:NME8
Gene Synonym:
Exon (11)994..1190
Gene:NME8
Gene Synonym:
Exon (12)1191..1366
Gene:NME8
Gene Synonym:
Exon (13)1367..1511
Gene:NME8
Gene Synonym:
Exon (14)1512..1619
Gene:NME8
Gene Synonym:
Exon (15)1620..1771
Gene:NME8
Gene Synonym:
Exon (16)1772..1916
Gene:NME8
Gene Synonym:
Exon (17)1917..2154
Gene:NME8
Gene Synonym:
Exon (18)2155..2312
Gene:NME8
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_016616
Organism Homo sapiens (human)
Definition Homo sapiens NME/NM23 family member 8 (NME8), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_016616

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCAAGCA AAAAACGAGA AGTCCAGTTA CAGACAGTCA TCAATAATCA AAGCCTGTGG 
GATGAGATGT TGCAGAACAA AGGCTTAACA GTGATTGATG TTTACCAAGC CTGGTGTGGA
CCTTGCAGAG CAATGCAACC TTTATTCAGA AAATTGAAAA ATGAACTGAA CGAAGACGAA
ATTCTGCATT TTGCTGTCGC AGAAGCTGAC AACATTGTGA CTTTGCAGCC ATTTAGAGAT
AAATGTGAAC CTGTTTTTCT CTTTAGTGTT AATGGCAAAA TTATCGAAAA GATTCAGGGT
GCAAATGCAC CGCTTGTTAA TAAAAAAGTT ATTAATTTGA TCGATGAGGA GAGAAAAATT
GCAGCAGGTG AAATGGCTCG ACCTCAGTAT CCTGAAATTC CATTAGTAGA CTCAGATTCA
GAAGTTAGTG AAGAATCACC ATGTGAAAGT GTTCAGGAAT TATACAGTAT TGCTATTATC
AAACCGGATG CTGTGATTAG TAAAAAAGTT CTAGAAATTA AAAGAAAAAT TACCAAAGCT
GGATTTATTA TAGAAGCAGA GCATAAGACA GTGCTCACTG AAGAACAAGT TGTCAACTTC
TATAGTCGAA TAGCAGACCA GTGTGACTTC GAAGAGTTTG TCTCTTTTAT GACAAGTGGC
TTAAGCTATA TTCTAGTTGT ATCTCAAGGA AGTAAACACA ATCCTCCCTC TGAAGAAACC
GAACCACAGA CTGACACCGA ACCTAACGAA CGATCTGAGG ATCAACCTGA GGTCGAAGCC
CAGGTTACAC CTGGAATGAT GAAGAACAAA CAAGACAGTT TACAAGAATA TCTGGAAAGA
CAACATTTAG CTCAGCTCTG TGACATTGAA GAGGATGCAG CTAATGTTGC TAAGTTCATG
GATGCTTTCT TCCCCGATTT TAAAAAAATG AAAAGCATGA AATTAGAAAA GACATTGGCA
TTACTTCGAC CAAATCTCTT TCATGAAAGG AAAGATGATG TTTTGCGTAT TATTAAAGAT
GAAGACTTCA AAATACTGGA GCAAAGACAA GTAGTATTAT CGGAAAAAGA AGCACAAGCA
CTGTGCAAGG AATATGAAAA TGAAGACTAT TTTAATAAAC TTATAGAAAA CATGACCAGT
GGTCCATCTC TAGCCCTTGT TTTATTGAGA GACAATGGCT TGCAATACTG GAAACAATTA
CTGGGACCAA GAACTGTTGA AGAAGCCATT GAATATTTTC CAGAGAGTTT ATGTGCACAG
TTTGCGATGG ACAGTTTGCC GGTCAACCAG TTGTATGGCA GCGATTCATT AGAAACCGCT
GAAAGGGAAA TACAGCATTT CTTTCCTCTT CAAAGCACTT TAGGCTTGAT TAAACCTCAT
GCAACAAGTG AACAAAGAGA GCAGATCCTG AAGATAGTTA AGGAGGCTGG ATTTGATCTG
ACACAGGTGA AGAAAATGTT CCTAACTCCT GAGCAAATAG AGAAAATTTA TCCAAAAGTA
ACAGGAAAAG ACTTTTATAA AGATTTATTG GAAATGTTAT CTGTGGGTCC ATCTATGGTC
ATGATTCTGA CCAAGTGGAA TGCTGTTGCA GAATGGAGAC GATTGATGGG CCCAACAGAC
CCAGAAGAAG CAAAATTACT TTCCCCTGAC TCCATCCGAG CCCAGTTTGG AATAAGTAAA
TTGAAAAACA TTGTCCATGG AGCATCTAAC GCCTATGAAG CAAAAGAGGT TGTTAATAGA
CTCTTTGAGG ATCCTGAGGA AAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Meta-analysis of 74,046 individuals identifies 11 new susceptibility loci for Alzheimer's disease
Nat. Genet. 45 (12), 1452-1458 (2013)
Lambert JC, Ibrahim-Verbaas CA, Harold D, Naj AC, Sims R, Bellenguez C, DeStafano AL, Bis JC, Beecham GW, Granier-Boley B, Russo G, Thorton-Wells TA, Jones N, Smith AV, Chouraki V, Thomas C, Ikram MA, Zelenika D, Vardarajan BN, Kamatani Y, Lin CF, Gerrish A, Schmidt H, Kunkle B, Dunstan ML, Ruiz A, Bihoreau MT, Choi SH, Reitz C, Pasquier F, Cruchaga C, Craig D, Amin N, Berr C, Lopez OL, De Jager PL, Deramecourt V, Johnston JA, Evans D, Lovestone S, Letenneur L, Moron FJ, Rubinsztein DC, Eiriksdottir G, Sleegers K, Goate AM, Fievet N, Huentelman MW, Gill M, Brown K, Kamboh MI, Keller L, Barberger-Gateau P, McGuiness B, Larson EB, Green R, Myers AJ, Dufouil C, Todd S, Wallon D, Love S, Rogaeva E, Gallacher J, St George-Hyslop P, Clarimon J, Lleo A, Bayer A, Tsuang DW, Yu L, Tsolaki M, Bossu P, Spalletta G, Proitsi P, Collinge J, Sorbi S, Sanchez-Garcia F, Fox NC, Hardy J, Deniz Naranjo MC, Bosco P, Clarke R, Brayne C, Galimberti D, Mancuso M, Matthews F, Moebus S, Mecocci P, Del Zompo M, Maier W, Hampel H, Pilotto A, Bullido M, Panza F, Caffarra P, Nacmias B, Gilbert JR, Mayhaus M, Lannefelt L, Hakonarson H, Pichler S, Carrasquillo MM, Ingelsson M, Beekly D, Alvarez V, Zou F, Valladares O, Younkin SG, Coto E, Hamilton-Nelson KL, Gu W, Razquin C, Pastor P, Mateo I, Owen MJ, Faber KM, Jonsson PV, Combarros O, O'Donovan MC, Cantwell LB, Soininen H, Blacker D, Mead S, Mosley TH Jr, Bennett DA, Harris TB, Fratiglioni L, Holmes C, de Bruijn RF, Passmore P, Montine TJ, Bettens K, Rotter JI, Brice A, Morgan K, Foroud TM, Kukull WA, Hannequin D, Powell JF, Nalls MA, Ritchie K, Lunetta KL, Kauwe JS, Boerwinkle E, Riemenschneider M, Boada M, Hiltuenen M, Martin ER, Schmidt R, Rujescu D, Wang LS, Dartigues JF, Mayeux R, Tzourio C, Hofman A, Nothen MM, Graff C, Psaty BM, Jones L, Haines JL, Holmans PA, Lathrop M, Pericak-Vance MA, Launer LJ, Farrer LA, van Duijn CM, Van Broeckhoven C, Moskvina V, Seshadri S, Williams J, Schellenberg GD and Amouyel P.


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Genome-wide meta-analysis identifies 56 bone mineral density loci and reveals 14 loci associated with risk of fracture
Nat. Genet. 44 (5), 491-501 (2012)
Estrada K, Styrkarsdottir U, Evangelou E, Hsu YH, Duncan EL, Ntzani EE, Oei L, Albagha OM, Amin N, Kemp JP, Koller DL, Li G, Liu CT, Minster RL, Moayyeri A, Vandenput L, Willner D, Xiao SM, Yerges-Armstrong LM, Zheng HF, Alonso N, Eriksson J, Kammerer CM, Kaptoge SK, Leo PJ, Thorleifsson G, Wilson SG, Wilson JF, Aalto V, Alen M, Aragaki AK, Aspelund T, Center JR, Dailiana Z, Duggan DJ, Garcia M, Garcia-Giralt N, Giroux S, Hallmans G, Hocking LJ, Husted LB, Jameson KA, Khusainova R, Kim GS, Kooperberg C, Koromila T, Kruk M, Laaksonen M, Lacroix AZ, Lee SH, Leung PC, Lewis JR, Masi L, Mencej-Bedrac S, Nguyen TV, Nogues X, Patel MS, Prezelj J, Rose LM, Scollen S, Siggeirsdottir K, Smith AV, Svensson O, Trompet S, Trummer O, van Schoor NM, Woo J, Zhu K, Balcells S, Brandi ML, Buckley BM, Cheng S, Christiansen C, Cooper C, Dedoussis G, Ford I, Frost M, Goltzman D, Gonzalez-Macias J, Kahonen M, Karlsson M, Khusnutdinova E, Koh JM, Kollia P, Langdahl BL, Leslie WD, Lips P, Ljunggren O, Lorenc RS, Marc J, Mellstrom D, Obermayer-Pietsch B, Olmos JM, Pettersson-Kymmer U, Reid DM, Riancho JA, Ridker PM, Rousseau F, Slagboom PE, Tang NL, Urreizti R, Van Hul W, Viikari J, Zarrabeitia MT, Aulchenko YS, Castano-Betancourt M, Grundberg E, Herrera L, Ingvarsson T, Johannsdottir H, Kwan T, Li R, Luben R, Medina-Gomez C, Palsson ST, Reppe S, Rotter JI, Sigurdsson G, van Meurs JB, Verlaan D, Williams FM, Wood AR, Zhou Y, Gautvik KM, Pastinen T, Raychaudhuri S, Cauley JA, Chasman DI, Clark GR, Cummings SR, Danoy P, Dennison EM, Eastell R, Eisman JA, Gudnason V, Hofman A, Jackson RD, Jones G, Jukema JW, Khaw KT, Lehtimaki T, Liu Y, Lorentzon M, McCloskey E, Mitchell BD, Nandakumar K, Nicholson GC, Oostra BA, Peacock M, Pols HA, Prince RL, Raitakari O, Reid IR, Robbins J, Sambrook PN, Sham PC, Shuldiner AR, Tylavsky FA, van Duijn CM, Wareham NJ, Cupples LA, Econs MJ, Evans DM, Harris TB, Kung AW, Psaty BM, Reeve J, Spector TD, Streeten EA, Zillikens MC, Thorsteinsdottir U, Ohlsson C, Karasik D, Richards JB, Brown MA, Stefansson K, Uitterlinden AG, Ralston SH, Ioannidis JP, Kiel DP and Rivadeneira F.


book

Field synopsis and synthesis of genetic association studies in osteoarthritis: the CUMAGAS-OSTEO information system
Am. J. Epidemiol. 171 (8), 851-858 (2010)
Zintzaras E, Kitsios GD, Ziogas DC, Rodopoulou P and Karachalios T.


book

Nme protein family evolutionary history, a vertebrate perspective
BMC Evol. Biol. 9, 256 (2009)
Desvignes T, Pontarotti P, Fauvel C and Bobe J.


book

A common variant in combination with a nonsense mutation in a member of the thioredoxin family causes primary ciliary dyskinesia
Proc. Natl. Acad. Sci. U.S.A. 104 (9), 3336-3341 (2007)
Duriez B, Duquesnoy P, Escudier E, Bridoux AM, Escalier D, Rayet I, Marcos E, Vojtek AM, Bercher JF and Amselem S.


book

Cis- and trans-acting gene regulation is associated with osteoarthritis
Am. J. Hum. Genet. 78 (5), 793-803 (2006)
Mahr S, Burmester GR, Hilke D, Gobel U, Grutzkau A, Haupl T, Hauschild M, Koczan D, Krenn V, Neidel J, Perka C, Radbruch A, Thiesen HJ and Muller B.


book

Sptrx-2, a fusion protein composed of one thioredoxin and three tandemly repeated NDP-kinase domains is expressed in human testis germ cells
Genes Cells 6 (12), 1077-1090 (2001)
Sadek CM, Damdimopoulos AE, Pelto-Huikko M, Gustafsson JA, Spyrou G and Miranda-Vizuete A.


book

Molecular cloning and characterization of a thioredoxin/nucleoside diphosphate kinase related dynein intermediate chain from the ascidian, Ciona intestinalis
Gene 275 (1), 177-183 (2001)
Padma P, Hozumi A, Ogawa K and Inaba K.


book

The human Nm23/nucleoside diphosphate kinases
J. Bioenerg. Biomembr. 32 (3), 247-258 (2000)
Lacombe ML, Milon L, Munier A, Mehus JG and Lambeth DO.


book

Primary Ciliary Dyskinesia
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Zariwala,M.A., Knowles,M.R. and Leigh,M.W.


 
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