×

ATP5F1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol ATP5F1
Entrez Gene ID 515
Full Name ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1
Synonyms PIG47
General protein information
Preferred Names
ATP synthase subunit b, mitochondrial
Names
ATP synthase subunit b, mitochondrial
ATPase subunit b
H+-ATP synthase subunit b
ATP synthase B chain, mitochondrial
cell proliferation-inducing protein 47
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1
NP_001679.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p13.2

Summary This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

603270


mRNA and Protein(s)

mRNA Protein Name
NM_001688 NP_001679 ATP synthase F(0) complex subunit B1, mitochondrial precursor



Homo sapiens (human) ATP5F1 NP_001679.2
Pan troglodytes (chimpanzee) ATP5F1 XP_513639.1
Macaca mulatta (Rhesus monkey) ATP5F1 XP_001105535.1
Canis lupus familiaris (dog) LOC479901 XP_537027.2
Bos taurus (cattle) ATP5F1 NP_001033590.1
Mus musculus (house mouse) Atp5f1 NP_033855.2
Rattus norvegicus (Norway rat) Atp5f1 NP_599192.1
Rattus norvegicus (Norway rat) LOC100911417 XP_003749420.1
Gallus gallus (chicken) ATP5F1 XP_417993.3
Danio rerio (zebrafish) atp5f1 NP_001005960.1
Drosophila melanogaster (fruit fly) ATPsyn-b NP_524011.1
Xenopus (Silurana) tropicalis (western clawed frog) atp5f1 NP_989118.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ATP5F1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP5F1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu21926
NM_001688 Homo sapiens ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1 (ATP5F1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu21926
Clone ID Related Accession (Same CDS sequence) NM_001688
Accession Version NM_001688.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 771bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ATP synthase F(0) complex subunit B1, mitochondrial precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF237506.1, AL390195.10, AW778751.1 and AI754631.1. On Jan 27, 2006 this sequence version replaced gi:50593508. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK291077.1, BC016350.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGCTGTCCC GGGTGGTACT TTCCGCCGCC GCCACAGCGG CCCCCTCTCT GAAGAATGCA 
GCCTTCCTAG GTCCAGGGGT ATTGCAGGCA ACAAGGACCT TTCATACAGG GCAGCCACAC
CTTGTCCCTG TACCACCTCT TCCTGAATAC GGAGGAAAAG TTCGTTATGG ACTGATCCCT
GAGGAATTCT TCCAGTTTCT TTATCCTAAA ACTGGTGTAA CAGGACCCTA TGTACTCGGA
ACTGGGCTTA TCTTGTACGC TTTATCCAAA GAAATATATG TGATTAGCGC AGAGACCTTC
ACTGCCCTAT CAGTACTAGG TGTAATGGTC TATGGAATTA AAAAATATGG TCCCTTTGTT
GCAGACTTTG CTGATAAACT CAATGAGCAA AAACTTGCCC AACTAGAAGA GGCGAAGCAG
GCTTCCATCC AACACATCCA GAATGCAATT GATACGGAGA AGTCACAACA GGCACTGGTT
CAGAAGCGCC ATTACCTTTT TGATGTGCAA AGGAATAACA TTGCTATGGC TTTGGAAGTT
ACTTACCGGG AACGACTGTA TAGAGTATAT AAGGAAGTAA AGAATCGCCT GGACTATCAT
ATATCTGTGC AGAACATGAT GCGTCGAAAG GAACAAGAAC ACATGATAAA TTGGGTGGAG
AAGCACGTGG TGCAAAGCAT CTCCACACAG CAGGAAAAGG AGACAATTGC CAAGTGCATT
GCGGACCTAA AGCTGCTGGC AAAGAAGGCT CAAGCACAGC CAGTTATGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001679.2
CDS422..1192
Misc Feature(1)23..25(+)
Misc Feature(2)668..1153(+)
Misc Feature(3)1082..1084(+)
Misc Feature(4)1118..1120(+)
Exon (1)1..461
Gene:ATP5F1
Gene Synonym:
Exon (2)462..498
Gene:ATP5F1
Gene Synonym:
Exon (3)499..644
Gene:ATP5F1
Gene Synonym:
Exon (4)645..808
Gene:ATP5F1
Gene Synonym:
Exon (5)809..934
Gene:ATP5F1
Gene Synonym:
Exon (6)935..1114
Gene:ATP5F1
Gene Synonym:
Exon (7)1115..2116
Gene:ATP5F1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001688
Organism Homo sapiens (human)
Definition Homo sapiens ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1 (ATP5F1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001688

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGCTGTCCC GGGTGGTACT TTCCGCCGCC GCCACAGCGG CCCCCTCTCT GAAGAATGCA 
GCCTTCCTAG GTCCAGGGGT ATTGCAGGCA ACAAGGACCT TTCATACAGG GCAGCCACAC
CTTGTCCCTG TACCACCTCT TCCTGAATAC GGAGGAAAAG TTCGTTATGG ACTGATCCCT
GAGGAATTCT TCCAGTTTCT TTATCCTAAA ACTGGTGTAA CAGGACCCTA TGTACTCGGA
ACTGGGCTTA TCTTGTACGC TTTATCCAAA GAAATATATG TGATTAGCGC AGAGACCTTC
ACTGCCCTAT CAGTACTAGG TGTAATGGTC TATGGAATTA AAAAATATGG TCCCTTTGTT
GCAGACTTTG CTGATAAACT CAATGAGCAA AAACTTGCCC AACTAGAAGA GGCGAAGCAG
GCTTCCATCC AACACATCCA GAATGCAATT GATACGGAGA AGTCACAACA GGCACTGGTT
CAGAAGCGCC ATTACCTTTT TGATGTGCAA AGGAATAACA TTGCTATGGC TTTGGAAGTT
ACTTACCGGG AACGACTGTA TAGAGTATAT AAGGAAGTAA AGAATCGCCT GGACTATCAT
ATATCTGTGC AGAACATGAT GCGTCGAAAG GAACAAGAAC ACATGATAAA TTGGGTGGAG
AAGCACGTGG TGCAAAGCAT CTCCACACAG CAGGAAAAGG AGACAATTGC CAAGTGCATT
GCGGACCTAA AGCTGCTGGC AAAGAAGGCT CAAGCACAGC CAGTTATGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Down-regulation of mitochondrial ATPase by hypermethylation mechanism in chronic myeloid leukemia is associated with multidrug resistance
Ann. Oncol. 21 (7), 1506-1514 (2010)
Li RJ, Zhang GS, Chen YH, Zhu JF, Lu QJ, Gong FJ and Kuang WY.


book

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression
PLoS ONE 5 (9), E12862 (2010)
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL and O'Brien SJ.


book

Nitration of tyrosine residues 368 and 345 in the beta-subunit elicits FoF1-ATPase activity loss
Biochem. J. 423 (2), 219-231 (2009)
Fujisawa Y, Kato K and Giulivi C.


book

Sequential use of transcriptional profiling, expression quantitative trait mapping, and gene association implicates MMP20 in human kidney aging
PLoS Genet. 5 (10), E1000685 (2009)
Wheeler HE, Metter EJ, Tanaka T, Absher D, Higgins J, Zahn JM, Wilhelmy J, Davis RW, Singleton A, Myers RM, Ferrucci L and Kim SK.


book

Polymorphisms in mitochondrial genes and prostate cancer risk
Cancer Epidemiol. Biomarkers Prev. 17 (12), 3558-3566 (2008)
Wang L, McDonnell SK, Hebbring SJ, Cunningham JM, St Sauver J, Cerhan JR, Isaya G, Schaid DJ and Thibodeau SN.


book

Energy transduction in ATP synthase
Nature 391 (6666), 510-513 (1998)
Elston T, Wang H and Oster G.


book

The expression of subunit c correlates with and thus may limit the biosynthesis of the mitochondrial F0F1-ATPase in brown adipose tissue
J. Biol. Chem. 270 (13), 7689-7694 (1995)
Houstek J, Andersson U, Tvrdik P, Nedergaard J and Cannon B.


book

Construction of a human full-length cDNA bank
Gene 150 (2), 243-250 (1994)
Kato S, Sekine S, Oh SW, Kim NS, Umezawa Y, Abe N, Yokoyama-Kobayashi M and Aoki T.


book

Molecular cloning of cDNA for the import precursor of human subunit B of H(+)-ATP synthase in mitochondria
Biochem. Biophys. Res. Commun. 178 (3), 1014-1020 (1991)
Higuti T, Tsurumi C, Osaka F, Kawamura Y, Tsujita H, Yoshihara Y, Tani I, Tanaka K and Ichihara A.


book

A new concept for energy coupling in oxidative phosphorylation based on a molecular explanation of the oxygen exchange reactions
Proc. Natl. Acad. Sci. U.S.A. 70 (10), 2837-2839 (1973)
Boyer,P.D., Cross,R.L. and Momsen,W.


 
*
*
*
*