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PDHA1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol PDHA1
Entrez Gene ID 5160
Full Name pyruvate dehydrogenase (lipoamide) alpha 1
Synonyms PDHA, PDHAD, PDHCE1A, PHE1A
General protein information
Preferred Names
pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
Names
pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
PDHE1-A type I
pyruvate dehydrogenase complex, E1-alpha polypeptide 1
NP_000275.1
NP_001166925.1
NP_001166926.1
NP_001166927.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xp22.1

Summary The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]. lac of sum
Disorder MIM:

300502

Disorder Html: Pyruvate dehydrogenase deficiency, 312170 (3); Leigh syndrome,

mRNA and Protein(s)

mRNA Protein Name
XM_011545531 XP_011543833 pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform X1
XM_011545532 XP_011543834 pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform X2
NM_001173456 NP_001166927 pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform 4 precursor
NM_001173454 NP_001166925 pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform 2 precursor
NM_001173455 NP_001166926 pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform 3 precursor
NM_000284 NP_000275 pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform 1 precursor



Homo sapiens (human) PDHA1 NP_001166926.1
Pan troglodytes (chimpanzee) PDHA1 NP_001104283.1
Macaca mulatta (Rhesus monkey) LOC719277 XP_001113273.2
Canis lupus familiaris (dog) PDHA1 XP_003435519.1
Bos taurus (cattle) PDHA1 NP_001094516.1
Mus musculus (house mouse) Pdha1 NP_032836.1
Rattus norvegicus (Norway rat) Pdha1 NP_001004072.2
Gallus gallus (chicken) PDHA1 NP_001012562.1
Danio rerio (zebrafish) pdha1b NP_001002399.1
Danio rerio (zebrafish) pdha1a NP_998558.1
Caenorhabditis elegans pdha-1 NP_871953.1
Arabidopsis thaliana (thale cress) IAR4 NP_173828.1
Arabidopsis thaliana (thale cress) E1 ALPHA NP_176198.1
Xenopus (Silurana) tropicalis (western clawed frog) pdha1 NP_001017072.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PDHA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDHA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu59599 XM_011545531 PREDICTED: Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu59600 XM_011545532 PREDICTED: Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu10616
NM_001173456 Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$149.50-$209.30
$299.00
OHu11172 NM_001173454 Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $251.30
$359.00
OHu11667 NM_001173455 Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $237.30
$339.00
OHu20621
NM_000284 Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu59599
Clone ID Related Accession (Same CDS sequence) XM_011545531
Accession Version XM_011545531.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1308bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167197.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGAGA 
CATGGTCTTG CTACGTTGCC CAGTCTGGTC TCCATCTCCA GGCTCAAGCA GTCCTCCCAC
CTCGGCCTCC CAAAGTGCTG GGATTACTCT CACTCTCTTA AAACCAGGCA GGCAAGCAGA
GTGCTGGTAG CATCCCGTAA TTTTGCAAAT GATGCTACAT TTGAAATTAA GAAATGTGAC
CTTCACCGGC TGGAAGAAGG CCCTCCTGTC ACAACAGTGC TCACCAGGGA GGATGGGCTC
AAATACTACA GGATGATGCA GACTGTACGC CGAATGGAGT TGAAAGCAGA TCAGCTGTAT
AAACAGAAAA TTATTCGTGG TTTCTGTCAC TTGTGTGATG GTCAGTTTCT CCTTCCTCTA
ACACAGGAAG CTTGCTGTGT GGGCCTGGAG GCCGGCATCA ACCCCACAGA CCATCTCATC
ACAGCCTACC GGGCTCACGG CTTTACTTTC ACCCGGGGCC TTTCCGTCCG AGAAATTCTC
GCAGAGCTTA CAGGACGAAA AGGAGGTTGT GCTAAAGGGA AAGGAGGATC GATGCACATG
TATGCCAAGA ACTTCTACGG GGGCAATGGC ATCGTGGGAG CGCAGGTGCC CCTGGGCGCT
GGGATTGCTC TAGCCTGTAA GTATAATGGA AAAGATGAGG TCTGCCTGAC TTTATATGGC
GATGGTGCTG CTAACCAGGG CCAGATATTC GAAGCTTACA ACATGGCAGC TTTGTGGAAA
TTACCTTGTA TTTTCATCTG TGAGAATAAT CGCTATGGAA TGGGAACGTC TGTTGAGAGA
GCGGCAGCCA GCACTGATTA CTACAAGAGA GGCGATTTCA TTCCTGGGCT GAGAGTGGAT
GGAATGGATA TCCTGTGCGT CCGAGAGGCA ACAAGGTTTG CTGCTGCCTA TTGTAGATCT
GGGAAGGGGC CCATCCTGAT GGAGCTGCAG ACTTACCGTT ACCACGGACA CAGTATGAGT
GACCCTGGAG TCAGTTACCG TACACGAGAA GAAATTCAGG AAGTAAGAAG TAAGAGTGAC
CCTATTATGC TTCTCAAGGA CAGGATGGTG AACAGCAATC TTGCCAGTGT GGAAGAACTA
AAGGAAATTG ATGTGGAAGT GAGGAAGGAG ATTGAGGATG CTGCCCAGTT TGCCACGGCC
GATCCTGAGC CACCTTTGGA AGAGCTGGGC TACCACATCT ACTCCAGCGA CCCACCTTTT
GAAGTTCGTG GTGCCAATCA GTGGATCAAG TTTAAGTCAG TCAGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011543833.1
CDS133..1440
Misc Feature(1)436..1326(+)
Misc Feature(2)508..1167(+)
Misc Feature(3)619..1143(+)
Misc Feature(4)745..906(+)
Misc Feature(5)1126..1215(+)
Translation

Target ORF information:

RefSeq Version XM_011545531
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011545531

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGAGA 
CATGGTCTTG CTACGTTGCC CAGTCTGGTC TCCATCTCCA GGCTCAAGCA GTCCTCCCAC
CTCGGCCTCC CAAAGTGCTG GGATTACTCT CACTCTCTTA AAACCAGGCA GGCAAGCAGA
GTGCTGGTAG CATCCCGTAA TTTTGCAAAT GATGCTACAT TTGAAATTAA GAAATGTGAC
CTTCACCGGC TGGAAGAAGG CCCTCCTGTC ACAACAGTGC TCACCAGGGA GGATGGGCTC
AAATACTACA GGATGATGCA GACTGTACGC CGAATGGAGT TGAAAGCAGA TCAGCTGTAT
AAACAGAAAA TTATTCGTGG TTTCTGTCAC TTGTGTGATG GTCAGTTTCT CCTTCCTCTA
ACACAGGAAG CTTGCTGTGT GGGCCTGGAG GCCGGCATCA ACCCCACAGA CCATCTCATC
ACAGCCTACC GGGCTCACGG CTTTACTTTC ACCCGGGGCC TTTCCGTCCG AGAAATTCTC
GCAGAGCTTA CAGGACGAAA AGGAGGTTGT GCTAAAGGGA AAGGAGGATC GATGCACATG
TATGCCAAGA ACTTCTACGG GGGCAATGGC ATCGTGGGAG CGCAGGTGCC CCTGGGCGCT
GGGATTGCTC TAGCCTGTAA GTATAATGGA AAAGATGAGG TCTGCCTGAC TTTATATGGC
GATGGTGCTG CTAACCAGGG CCAGATATTC GAAGCTTACA ACATGGCAGC TTTGTGGAAA
TTACCTTGTA TTTTCATCTG TGAGAATAAT CGCTATGGAA TGGGAACGTC TGTTGAGAGA
GCGGCAGCCA GCACTGATTA CTACAAGAGA GGCGATTTCA TTCCTGGGCT GAGAGTGGAT
GGAATGGATA TCCTGTGCGT CCGAGAGGCA ACAAGGTTTG CTGCTGCCTA TTGTAGATCT
GGGAAGGGGC CCATCCTGAT GGAGCTGCAG ACTTACCGTT ACCACGGACA CAGTATGAGT
GACCCTGGAG TCAGTTACCG TACACGAGAA GAAATTCAGG AAGTAAGAAG TAAGAGTGAC
CCTATTATGC TTCTCAAGGA CAGGATGGTG AACAGCAATC TTGCCAGTGT GGAAGAACTA
AAGGAAATTG ATGTGGAAGT GAGGAAGGAG ATTGAGGATG CTGCCCAGTT TGCCACGGCC
GATCCTGAGC CACCTTTGGA AGAGCTGGGC TACCACATCT ACTCCAGCGA CCCACCTTTT
GAAGTTCGTG GTGCCAATCA GTGGATCAAG TTTAAGTCAG TCAGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59600
Clone ID Related Accession (Same CDS sequence) XM_011545532
Accession Version XM_011545532.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1215bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167197.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGAGA 
CATGGTCTTG CTACGTTGCC CAGTCTGGTC TCCATCTCCA GGCTCAAGCA GTCCTCCCAC
CTCGGCCTCC CAAAGTGCTG GGATTACTCT CACTCTCTTA AAACCAGGCA GGCAAGCAGA
GTGCTGGTAG CATCCCGTAA TTTTGCAAAT GATGCTACAT TTGAAATTAA GAAATGTGAC
CTTCACCGGC TGGAAGAAGG CCCTCCTGTC ACAACAGTGC TCACCAGGGA GGATGGGCTC
AAATACTACA GGATGATGCA GACTGTACGC CGAATGGAGT TGAAAGCAGA TCAGCTGTAT
AAACAGAAAA TTATTCGTGG TTTCTGTCAC TTGTGTGATG GTCAGTTTCT CCTTCCTCTA
ACACAGGAAG CTTGCTGTGT GGGCCTGGAG GCCGGCATCA ACCCCACAGA CCATCTCATC
ACAGCCTACC GGGCTCACGG CTTTACTTTC ACCCGGGGCC TTTCCGTCCG AGAAATTCTC
GCAGAGCTTA CAGGACGAAA AGGAGGTTGT GCTAAAGGGA AAGGAGGATC GATGCACATG
TATGCCAAGA ACTTCTACGG GGGCAATGGC ATCGTGGGAG CGCAGGGCCA GATATTCGAA
GCTTACAACA TGGCAGCTTT GTGGAAATTA CCTTGTATTT TCATCTGTGA GAATAATCGC
TATGGAATGG GAACGTCTGT TGAGAGAGCG GCAGCCAGCA CTGATTACTA CAAGAGAGGC
GATTTCATTC CTGGGCTGAG AGTGGATGGA ATGGATATCC TGTGCGTCCG AGAGGCAACA
AGGTTTGCTG CTGCCTATTG TAGATCTGGG AAGGGGCCCA TCCTGATGGA GCTGCAGACT
TACCGTTACC ACGGACACAG TATGAGTGAC CCTGGAGTCA GTTACCGTAC ACGAGAAGAA
ATTCAGGAAG TAAGAAGTAA GAGTGACCCT ATTATGCTTC TCAAGGACAG GATGGTGAAC
AGCAATCTTG CCAGTGTGGA AGAACTAAAG GAAATTGATG TGGAAGTGAG GAAGGAGATT
GAGGATGCTG CCCAGTTTGC CACGGCCGAT CCTGAGCCAC CTTTGGAAGA GCTGGGCTAC
CACATCTACT CCAGCGACCC ACCTTTTGAA GTTCGTGGTG CCAATCAGTG GATCAAGTTT
AAGTCAGTCA GTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011543834.1
CDS133..1347
Misc Feature(1)436..1233(+)
Misc Feature(2)508..1074(+)
Misc Feature(3)619..1050(+)
Misc Feature(4)745..813(+)
Misc Feature(5)1033..1122(+)
Translation

Target ORF information:

RefSeq Version XM_011545532
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011545532

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGAGA 
CATGGTCTTG CTACGTTGCC CAGTCTGGTC TCCATCTCCA GGCTCAAGCA GTCCTCCCAC
CTCGGCCTCC CAAAGTGCTG GGATTACTCT CACTCTCTTA AAACCAGGCA GGCAAGCAGA
GTGCTGGTAG CATCCCGTAA TTTTGCAAAT GATGCTACAT TTGAAATTAA GAAATGTGAC
CTTCACCGGC TGGAAGAAGG CCCTCCTGTC ACAACAGTGC TCACCAGGGA GGATGGGCTC
AAATACTACA GGATGATGCA GACTGTACGC CGAATGGAGT TGAAAGCAGA TCAGCTGTAT
AAACAGAAAA TTATTCGTGG TTTCTGTCAC TTGTGTGATG GTCAGTTTCT CCTTCCTCTA
ACACAGGAAG CTTGCTGTGT GGGCCTGGAG GCCGGCATCA ACCCCACAGA CCATCTCATC
ACAGCCTACC GGGCTCACGG CTTTACTTTC ACCCGGGGCC TTTCCGTCCG AGAAATTCTC
GCAGAGCTTA CAGGACGAAA AGGAGGTTGT GCTAAAGGGA AAGGAGGATC GATGCACATG
TATGCCAAGA ACTTCTACGG GGGCAATGGC ATCGTGGGAG CGCAGGGCCA GATATTCGAA
GCTTACAACA TGGCAGCTTT GTGGAAATTA CCTTGTATTT TCATCTGTGA GAATAATCGC
TATGGAATGG GAACGTCTGT TGAGAGAGCG GCAGCCAGCA CTGATTACTA CAAGAGAGGC
GATTTCATTC CTGGGCTGAG AGTGGATGGA ATGGATATCC TGTGCGTCCG AGAGGCAACA
AGGTTTGCTG CTGCCTATTG TAGATCTGGG AAGGGGCCCA TCCTGATGGA GCTGCAGACT
TACCGTTACC ACGGACACAG TATGAGTGAC CCTGGAGTCA GTTACCGTAC ACGAGAAGAA
ATTCAGGAAG TAAGAAGTAA GAGTGACCCT ATTATGCTTC TCAAGGACAG GATGGTGAAC
AGCAATCTTG CCAGTGTGGA AGAACTAAAG GAAATTGATG TGGAAGTGAG GAAGGAGATT
GAGGATGCTG CCCAGTTTGC CACGGCCGAT CCTGAGCCAC CTTTGGAAGA GCTGGGCTAC
CACATCTACT CCAGCGACCC ACCTTTTGAA GTTCGTGGTG CCAATCAGTG GATCAAGTTT
AAGTCAGTCA GTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10616
Clone ID Related Accession (Same CDS sequence) NM_001173456
Accession Version NM_001173456.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1080bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 21-MAY-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform 4 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC361191.1, AK296457.1, AK296341.1, AL732326.4 and R49470.1. Transcript Variant: This variant (4) is missing an in-frame coding exon compared to variant 1, resulting in a shorter isoform (4) compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296457.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGGCA 
AGCAGAGTGC TGGTAGCATC CCGTAATTTT GCAAATGATG CTACATTTGA AATTAAGAAA
TGTGACCTTC ACCGGCTGGA AGAAGGCCCT CCTGTCACAA CAGTGCTCAC CAGGGAGGAT
GGGCTCAAAT ACTACAGGAT GATGCAGACT GTACGCCGAA TGGAGTTGAA AGCAGATCAG
CTGTATAAAC AGAAAATTAT TCGTGGTTTC TGTCACTTGT GTGATGGTCA GGAAGCTTGC
TGTGTGGGCC TGGAGGCCGG CATCAACCCC ACAGACCATC TCATCACAGC CTACCGGGCT
CACGGCTTTA CTTTCACCCG GGGCCTTTCC GTCCGAGAAA TTCTCGCAGA GCTTACAGGA
CGAAAAGGAG GTTGTGCTAA AGGGAAAGGA GGATCGATGC ACATGTATGC CAAGAACTTC
TACGGGGGCA ATGGCATCGT GGGAGCGCAG GGCCAGATAT TCGAAGCTTA CAACATGGCA
GCTTTGTGGA AATTACCTTG TATTTTCATC TGTGAGAATA ATCGCTATGG AATGGGAACG
TCTGTTGAGA GAGCGGCAGC CAGCACTGAT TACTACAAGA GAGGCGATTT CATTCCTGGG
CTGAGAGTGG ATGGAATGGA TATCCTGTGC GTCCGAGAGG CAACAAGGTT TGCTGCTGCC
TATTGTAGAT CTGGGAAGGG GCCCATCCTG ATGGAGCTGC AGACTTACCG TTACCACGGA
CACAGTATGA GTGACCCTGG AGTCAGTTAC CGTACACGAG AAGAAATTCA GGAAGTAAGA
AGTAAGAGTG ACCCTATTAT GCTTCTCAAG GACAGGATGG TGAACAGCAA TCTTGCCAGT
GTGGAAGAAC TAAAGGAAAT TGATGTGGAA GTGAGGAAGG AGATTGAGGA TGCTGCCCAG
TTTGCCACGG CCGATCCTGA GCCACCTTTG GAAGAGCTGG GCTACCACAT CTACTCCAGC
GACCCACCTT TTGAAGTTCG TGGTGCCAAT CAGTGGATCA AGTTTAAGTC AGTCAGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001166927.1
CDS146..1225
Misc Feature(1)335..1111(+)
Misc Feature(2)407..952(+)
Misc Feature(3)497..928(+)
Misc Feature(4)623..691(+)
Misc Feature(5)911..1000(+)
Exon (1)1..202
Gene:PDHA1
Gene Synonym:
Exon (2)203..262
Gene:PDHA1
Gene Synonym:
Exon (3)263..436
Gene:PDHA1
Gene Synonym:
Exon (4)437..563
Gene:PDHA1
Gene Synonym:
Exon (5)564..655
Gene:PDHA1
Gene Synonym:
Exon (6)656..811
Gene:PDHA1
Gene Synonym:
Exon (7)812..883
Gene:PDHA1
Gene Synonym:
Exon (8)884..951
Gene:PDHA1
Gene Synonym:
Exon (9)952..1060
Gene:PDHA1
Gene Synonym:
Exon (10)1061..3279
Gene:PDHA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001173456
Organism Homo sapiens (human)
Definition Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001173456

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGGCA 
AGCAGAGTGC TGGTAGCATC CCGTAATTTT GCAAATGATG CTACATTTGA AATTAAGAAA
TGTGACCTTC ACCGGCTGGA AGAAGGCCCT CCTGTCACAA CAGTGCTCAC CAGGGAGGAT
GGGCTCAAAT ACTACAGGAT GATGCAGACT GTACGCCGAA TGGAGTTGAA AGCAGATCAG
CTGTATAAAC AGAAAATTAT TCGTGGTTTC TGTCACTTGT GTGATGGTCA GGAAGCTTGC
TGTGTGGGCC TGGAGGCCGG CATCAACCCC ACAGACCATC TCATCACAGC CTACCGGGCT
CACGGCTTTA CTTTCACCCG GGGCCTTTCC GTCCGAGAAA TTCTCGCAGA GCTTACAGGA
CGAAAAGGAG GTTGTGCTAA AGGGAAAGGA GGATCGATGC ACATGTATGC CAAGAACTTC
TACGGGGGCA ATGGCATCGT GGGAGCGCAG GGCCAGATAT TCGAAGCTTA CAACATGGCA
GCTTTGTGGA AATTACCTTG TATTTTCATC TGTGAGAATA ATCGCTATGG AATGGGAACG
TCTGTTGAGA GAGCGGCAGC CAGCACTGAT TACTACAAGA GAGGCGATTT CATTCCTGGG
CTGAGAGTGG ATGGAATGGA TATCCTGTGC GTCCGAGAGG CAACAAGGTT TGCTGCTGCC
TATTGTAGAT CTGGGAAGGG GCCCATCCTG ATGGAGCTGC AGACTTACCG TTACCACGGA
CACAGTATGA GTGACCCTGG AGTCAGTTAC CGTACACGAG AAGAAATTCA GGAAGTAAGA
AGTAAGAGTG ACCCTATTAT GCTTCTCAAG GACAGGATGG TGAACAGCAA TCTTGCCAGT
GTGGAAGAAC TAAAGGAAAT TGATGTGGAA GTGAGGAAGG AGATTGAGGA TGCTGCCCAG
TTTGCCACGG CCGATCCTGA GCCACCTTTG GAAGAGCTGG GCTACCACAT CTACTCCAGC
GACCCACCTT TTGAAGTTCG TGGTGCCAAT CAGTGGATCA AGTTTAAGTC AGTCAGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu11172
Clone ID Related Accession (Same CDS sequence) NM_001173454
Accession Version NM_001173454.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1287bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 21-MAY-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC361191.1, AK293250.1, BU170389.1, AL732326.4 and R49470.1. Transcript Variant: This variant (2) contains an additional in-frame coding exon compared to variant 1, resulting in a longer isoform (2) compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293250.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGAGA 
CATGGTCTTG CTACGTTGCC CAGTCTGGTC TCCATCTCCA GGCTCAAGCA GTCCTCCCAC
CTCGGCCTCC CAAAGTGCTG GGATTACTCT CACTCTCTTA AAACCAGGCA GGCAAGCAGA
GTGCTGGTAG CATCCCGTAA TTTTGCAAAT GATGCTACAT TTGAAATTAA GAAATGTGAC
CTTCACCGGC TGGAAGAAGG CCCTCCTGTC ACAACAGTGC TCACCAGGGA GGATGGGCTC
AAATACTACA GGATGATGCA GACTGTACGC CGAATGGAGT TGAAAGCAGA TCAGCTGTAT
AAACAGAAAA TTATTCGTGG TTTCTGTCAC TTGTGTGATG GTCAGGAAGC TTGCTGTGTG
GGCCTGGAGG CCGGCATCAA CCCCACAGAC CATCTCATCA CAGCCTACCG GGCTCACGGC
TTTACTTTCA CCCGGGGCCT TTCCGTCCGA GAAATTCTCG CAGAGCTTAC AGGACGAAAA
GGAGGTTGTG CTAAAGGGAA AGGAGGATCG ATGCACATGT ATGCCAAGAA CTTCTACGGG
GGCAATGGCA TCGTGGGAGC GCAGGTGCCC CTGGGCGCTG GGATTGCTCT AGCCTGTAAG
TATAATGGAA AAGATGAGGT CTGCCTGACT TTATATGGCG ATGGTGCTGC TAACCAGGGC
CAGATATTCG AAGCTTACAA CATGGCAGCT TTGTGGAAAT TACCTTGTAT TTTCATCTGT
GAGAATAATC GCTATGGAAT GGGAACGTCT GTTGAGAGAG CGGCAGCCAG CACTGATTAC
TACAAGAGAG GCGATTTCAT TCCTGGGCTG AGAGTGGATG GAATGGATAT CCTGTGCGTC
CGAGAGGCAA CAAGGTTTGC TGCTGCCTAT TGTAGATCTG GGAAGGGGCC CATCCTGATG
GAGCTGCAGA CTTACCGTTA CCACGGACAC AGTATGAGTG ACCCTGGAGT CAGTTACCGT
ACACGAGAAG AAATTCAGGA AGTAAGAAGT AAGAGTGACC CTATTATGCT TCTCAAGGAC
AGGATGGTGA ACAGCAATCT TGCCAGTGTG GAAGAACTAA AGGAAATTGA TGTGGAAGTG
AGGAAGGAGA TTGAGGATGC TGCCCAGTTT GCCACGGCCG ATCCTGAGCC ACCTTTGGAA
GAGCTGGGCT ACCACATCTA CTCCAGCGAC CCACCTTTTG AAGTTCGTGG TGCCAATCAG
TGGATCAAGT TTAAGTCAGT CAGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001166925.1
CDS146..1432
Misc Feature(1)449..1318(+)
Misc Feature(2)521..1159(+)
Misc Feature(3)611..1135(+)
Misc Feature(4)737..898(+)
Misc Feature(5)1118..1207(+)
Exon (1)1..202
Gene:PDHA1
Gene Synonym:
Exon (2)203..316
Gene:PDHA1
Gene Synonym:
Exon (3)317..376
Gene:PDHA1
Gene Synonym:
Exon (4)377..550
Gene:PDHA1
Gene Synonym:
Exon (5)551..677
Gene:PDHA1
Gene Synonym:
Exon (6)678..769
Gene:PDHA1
Gene Synonym:
Exon (7)770..862
Gene:PDHA1
Gene Synonym:
Exon (8)863..1018
Gene:PDHA1
Gene Synonym:
Exon (9)1019..1090
Gene:PDHA1
Gene Synonym:
Exon (10)1091..1158
Gene:PDHA1
Gene Synonym:
Exon (11)1159..1267
Gene:PDHA1
Gene Synonym:
Exon (12)1268..3486
Gene:PDHA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001173454
Organism Homo sapiens (human)
Definition Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001173454

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGAGA 
CATGGTCTTG CTACGTTGCC CAGTCTGGTC TCCATCTCCA GGCTCAAGCA GTCCTCCCAC
CTCGGCCTCC CAAAGTGCTG GGATTACTCT CACTCTCTTA AAACCAGGCA GGCAAGCAGA
GTGCTGGTAG CATCCCGTAA TTTTGCAAAT GATGCTACAT TTGAAATTAA GAAATGTGAC
CTTCACCGGC TGGAAGAAGG CCCTCCTGTC ACAACAGTGC TCACCAGGGA GGATGGGCTC
AAATACTACA GGATGATGCA GACTGTACGC CGAATGGAGT TGAAAGCAGA TCAGCTGTAT
AAACAGAAAA TTATTCGTGG TTTCTGTCAC TTGTGTGATG GTCAGGAAGC TTGCTGTGTG
GGCCTGGAGG CCGGCATCAA CCCCACAGAC CATCTCATCA CAGCCTACCG GGCTCACGGC
TTTACTTTCA CCCGGGGCCT TTCCGTCCGA GAAATTCTCG CAGAGCTTAC AGGACGAAAA
GGAGGTTGTG CTAAAGGGAA AGGAGGATCG ATGCACATGT ATGCCAAGAA CTTCTACGGG
GGCAATGGCA TCGTGGGAGC GCAGGTGCCC CTGGGCGCTG GGATTGCTCT AGCCTGTAAG
TATAATGGAA AAGATGAGGT CTGCCTGACT TTATATGGCG ATGGTGCTGC TAACCAGGGC
CAGATATTCG AAGCTTACAA CATGGCAGCT TTGTGGAAAT TACCTTGTAT TTTCATCTGT
GAGAATAATC GCTATGGAAT GGGAACGTCT GTTGAGAGAG CGGCAGCCAG CACTGATTAC
TACAAGAGAG GCGATTTCAT TCCTGGGCTG AGAGTGGATG GAATGGATAT CCTGTGCGTC
CGAGAGGCAA CAAGGTTTGC TGCTGCCTAT TGTAGATCTG GGAAGGGGCC CATCCTGATG
GAGCTGCAGA CTTACCGTTA CCACGGACAC AGTATGAGTG ACCCTGGAGT CAGTTACCGT
ACACGAGAAG AAATTCAGGA AGTAAGAAGT AAGAGTGACC CTATTATGCT TCTCAAGGAC
AGGATGGTGA ACAGCAATCT TGCCAGTGTG GAAGAACTAA AGGAAATTGA TGTGGAAGTG
AGGAAGGAGA TTGAGGATGC TGCCCAGTTT GCCACGGCCG ATCCTGAGCC ACCTTTGGAA
GAGCTGGGCT ACCACATCTA CTCCAGCGAC CCACCTTTTG AAGTTCGTGG TGCCAATCAG
TGGATCAAGT TTAAGTCAGT CAGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu11667
Clone ID Related Accession (Same CDS sequence) NM_001173455
Accession Version NM_001173455.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1194bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 21-MAY-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC361191.1, AK296341.1, AL732326.4 and R49470.1. Transcript Variant: This variant (3) uses an alternative acceptor splice site at one of the coding exons compared to variant 1, resulting in a longer isoform (3) compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296341.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGGCA 
AGCAGAGTGC TGGTAGCATC CCGTAATTTT GCAAATGATG CTACATTTGA AATTAAGAAA
TGTGACCTTC ACCGGCTGGA AGAAGGCCCT CCTGTCACAA CAGTGCTCAC CAGGGAGGAT
GGGCTCAAAT ACTACAGGAT GATGCAGACT GTACGCCGAA TGGAGTTGAA AGCAGATCAG
CTGTATAAAC AGAAAATTAT TCGTGGTTTC TGTCACTTGT GTGATGGTCA GTTTCTCCTT
CCTCTAACAC AGGAAGCTTG CTGTGTGGGC CTGGAGGCCG GCATCAACCC CACAGACCAT
CTCATCACAG CCTACCGGGC TCACGGCTTT ACTTTCACCC GGGGCCTTTC CGTCCGAGAA
ATTCTCGCAG AGCTTACAGG ACGAAAAGGA GGTTGTGCTA AAGGGAAAGG AGGATCGATG
CACATGTATG CCAAGAACTT CTACGGGGGC AATGGCATCG TGGGAGCGCA GGTGCCCCTG
GGCGCTGGGA TTGCTCTAGC CTGTAAGTAT AATGGAAAAG ATGAGGTCTG CCTGACTTTA
TATGGCGATG GTGCTGCTAA CCAGGGCCAG ATATTCGAAG CTTACAACAT GGCAGCTTTG
TGGAAATTAC CTTGTATTTT CATCTGTGAG AATAATCGCT ATGGAATGGG AACGTCTGTT
GAGAGAGCGG CAGCCAGCAC TGATTACTAC AAGAGAGGCG ATTTCATTCC TGGGCTGAGA
GTGGATGGAA TGGATATCCT GTGCGTCCGA GAGGCAACAA GGTTTGCTGC TGCCTATTGT
AGATCTGGGA AGGGGCCCAT CCTGATGGAG CTGCAGACTT ACCGTTACCA CGGACACAGT
ATGAGTGACC CTGGAGTCAG TTACCGTACA CGAGAAGAAA TTCAGGAAGT AAGAAGTAAG
AGTGACCCTA TTATGCTTCT CAAGGACAGG ATGGTGAACA GCAATCTTGC CAGTGTGGAA
GAACTAAAGG AAATTGATGT GGAAGTGAGG AAGGAGATTG AGGATGCTGC CCAGTTTGCC
ACGGCCGATC CTGAGCCACC TTTGGAAGAG CTGGGCTACC ACATCTACTC CAGCGACCCA
CCTTTTGAAG TTCGTGGTGC CAATCAGTGG ATCAAGTTTA AGTCAGTCAG TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001166926.1
CDS146..1339
Misc Feature(1)332..334(+)
Misc Feature(2)332..334(+)
Misc Feature(3)335..1225(+)
Misc Feature(4)407..1066(+)
Misc Feature(5)518..1042(+)
Misc Feature(6)644..805(+)
Misc Feature(7)860..862(+)
Misc Feature(8)896..898(+)
Misc Feature(9)896..898(+)
Misc Feature(10)995..997(+)
Misc Feature(11)1025..1114(+)
Misc Feature(12)1043..1045(+)
Misc Feature(13)1049..1051(+)
Misc Feature(14)1064..1066(+)
Misc Feature(15)1067..1069(+)
Misc Feature(16)1103..1105(+)
Misc Feature(17)1103..1105(+)
Misc Feature(18)1127..1129(+)
Misc Feature(19)1172..1174(+)
Misc Feature(20)1319..1321(+)
Exon (1)1..202
Gene:PDHA1
Gene Synonym:
Exon (2)203..262
Gene:PDHA1
Gene Synonym:
Exon (3)263..436
Gene:PDHA1
Gene Synonym:
Exon (4)437..584
Gene:PDHA1
Gene Synonym:
Exon (5)585..676
Gene:PDHA1
Gene Synonym:
Exon (6)677..769
Gene:PDHA1
Gene Synonym:
Exon (7)770..925
Gene:PDHA1
Gene Synonym:
Exon (8)926..997
Gene:PDHA1
Gene Synonym:
Exon (9)998..1065
Gene:PDHA1
Gene Synonym:
Exon (10)1066..1174
Gene:PDHA1
Gene Synonym:
Exon (11)1175..3393
Gene:PDHA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001173455
Organism Homo sapiens (human)
Definition Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001173455

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGGCA 
AGCAGAGTGC TGGTAGCATC CCGTAATTTT GCAAATGATG CTACATTTGA AATTAAGAAA
TGTGACCTTC ACCGGCTGGA AGAAGGCCCT CCTGTCACAA CAGTGCTCAC CAGGGAGGAT
GGGCTCAAAT ACTACAGGAT GATGCAGACT GTACGCCGAA TGGAGTTGAA AGCAGATCAG
CTGTATAAAC AGAAAATTAT TCGTGGTTTC TGTCACTTGT GTGATGGTCA GTTTCTCCTT
CCTCTAACAC AGGAAGCTTG CTGTGTGGGC CTGGAGGCCG GCATCAACCC CACAGACCAT
CTCATCACAG CCTACCGGGC TCACGGCTTT ACTTTCACCC GGGGCCTTTC CGTCCGAGAA
ATTCTCGCAG AGCTTACAGG ACGAAAAGGA GGTTGTGCTA AAGGGAAAGG AGGATCGATG
CACATGTATG CCAAGAACTT CTACGGGGGC AATGGCATCG TGGGAGCGCA GGTGCCCCTG
GGCGCTGGGA TTGCTCTAGC CTGTAAGTAT AATGGAAAAG ATGAGGTCTG CCTGACTTTA
TATGGCGATG GTGCTGCTAA CCAGGGCCAG ATATTCGAAG CTTACAACAT GGCAGCTTTG
TGGAAATTAC CTTGTATTTT CATCTGTGAG AATAATCGCT ATGGAATGGG AACGTCTGTT
GAGAGAGCGG CAGCCAGCAC TGATTACTAC AAGAGAGGCG ATTTCATTCC TGGGCTGAGA
GTGGATGGAA TGGATATCCT GTGCGTCCGA GAGGCAACAA GGTTTGCTGC TGCCTATTGT
AGATCTGGGA AGGGGCCCAT CCTGATGGAG CTGCAGACTT ACCGTTACCA CGGACACAGT
ATGAGTGACC CTGGAGTCAG TTACCGTACA CGAGAAGAAA TTCAGGAAGT AAGAAGTAAG
AGTGACCCTA TTATGCTTCT CAAGGACAGG ATGGTGAACA GCAATCTTGC CAGTGTGGAA
GAACTAAAGG AAATTGATGT GGAAGTGAGG AAGGAGATTG AGGATGCTGC CCAGTTTGCC
ACGGCCGATC CTGAGCCACC TTTGGAAGAG CTGGGCTACC ACATCTACTC CAGCGACCCA
CCTTTTGAAG TTCGTGGTGC CAATCAGTGG ATCAAGTTTA AGTCAGTCAG TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20621
Clone ID Related Accession (Same CDS sequence) NM_000284
Accession Version NM_000284.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1173bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 21-MAY-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC361191.1, L13318.1, BU170389.1, AL732326.4 and R49470.1. This sequence is a reference standard in the RefSeqGene project. On Mar 16, 2010 this sequence version replaced gi:164664523. Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L48690.1, L13318.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGGCA 
AGCAGAGTGC TGGTAGCATC CCGTAATTTT GCAAATGATG CTACATTTGA AATTAAGAAA
TGTGACCTTC ACCGGCTGGA AGAAGGCCCT CCTGTCACAA CAGTGCTCAC CAGGGAGGAT
GGGCTCAAAT ACTACAGGAT GATGCAGACT GTACGCCGAA TGGAGTTGAA AGCAGATCAG
CTGTATAAAC AGAAAATTAT TCGTGGTTTC TGTCACTTGT GTGATGGTCA GGAAGCTTGC
TGTGTGGGCC TGGAGGCCGG CATCAACCCC ACAGACCATC TCATCACAGC CTACCGGGCT
CACGGCTTTA CTTTCACCCG GGGCCTTTCC GTCCGAGAAA TTCTCGCAGA GCTTACAGGA
CGAAAAGGAG GTTGTGCTAA AGGGAAAGGA GGATCGATGC ACATGTATGC CAAGAACTTC
TACGGGGGCA ATGGCATCGT GGGAGCGCAG GTGCCCCTGG GCGCTGGGAT TGCTCTAGCC
TGTAAGTATA ATGGAAAAGA TGAGGTCTGC CTGACTTTAT ATGGCGATGG TGCTGCTAAC
CAGGGCCAGA TATTCGAAGC TTACAACATG GCAGCTTTGT GGAAATTACC TTGTATTTTC
ATCTGTGAGA ATAATCGCTA TGGAATGGGA ACGTCTGTTG AGAGAGCGGC AGCCAGCACT
GATTACTACA AGAGAGGCGA TTTCATTCCT GGGCTGAGAG TGGATGGAAT GGATATCCTG
TGCGTCCGAG AGGCAACAAG GTTTGCTGCT GCCTATTGTA GATCTGGGAA GGGGCCCATC
CTGATGGAGC TGCAGACTTA CCGTTACCAC GGACACAGTA TGAGTGACCC TGGAGTCAGT
TACCGTACAC GAGAAGAAAT TCAGGAAGTA AGAAGTAAGA GTGACCCTAT TATGCTTCTC
AAGGACAGGA TGGTGAACAG CAATCTTGCC AGTGTGGAAG AACTAAAGGA AATTGATGTG
GAAGTGAGGA AGGAGATTGA GGATGCTGCC CAGTTTGCCA CGGCCGATCC TGAGCCACCT
TTGGAAGAGC TGGGCTACCA CATCTACTCC AGCGACCCAC CTTTTGAAGT TCGTGGTGCC
AATCAGTGGA TCAAGTTTAA GTCAGTCAGT TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000275.1
CDS146..1318
Misc Feature(1)332..334(+)
Misc Feature(2)332..334(+)
Misc Feature(3)335..1204(+)
Misc Feature(4)407..1045(+)
Misc Feature(5)497..1021(+)
Misc Feature(6)623..784(+)
Misc Feature(7)839..841(+)
Misc Feature(8)839..841(+)
Misc Feature(9)839..841(+)
Misc Feature(10)839..841(+)
Misc Feature(11)839..841(+)
Misc Feature(12)875..877(+)
Misc Feature(13)875..877(+)
Misc Feature(14)974..976(+)
Misc Feature(15)1004..1093(+)
Misc Feature(16)1022..1024(+)
Misc Feature(17)1022..1024(+)
Misc Feature(18)1022..1024(+)
Misc Feature(19)1022..1024(+)
Misc Feature(20)1022..1024(+)
Misc Feature(21)1022..1024(+)
Misc Feature(22)1028..1030(+)
Misc Feature(23)1043..1045(+)
Misc Feature(24)1043..1045(+)
Misc Feature(25)1043..1045(+)
Misc Feature(26)1043..1045(+)
Misc Feature(27)1043..1045(+)
Misc Feature(28)1046..1048(+)
Misc Feature(29)1046..1048(+)
Misc Feature(30)1082..1084(+)
Misc Feature(31)1082..1084(+)
Misc Feature(32)1106..1108(+)
Misc Feature(33)1151..1153(+)
Misc Feature(34)1250..1252(+)
Misc Feature(35)1298..1300(+)
Exon (1)1..202
Gene:PDHA1
Gene Synonym:
Exon (2)203..262
Gene:PDHA1
Gene Synonym:
Exon (3)263..436
Gene:PDHA1
Gene Synonym:
Exon (4)437..563
Gene:PDHA1
Gene Synonym:
Exon (5)564..655
Gene:PDHA1
Gene Synonym:
Exon (6)656..748
Gene:PDHA1
Gene Synonym:
Exon (7)749..904
Gene:PDHA1
Gene Synonym:
Exon (8)905..976
Gene:PDHA1
Gene Synonym:
Exon (9)977..1044
Gene:PDHA1
Gene Synonym:
Exon (10)1045..1153
Gene:PDHA1
Gene Synonym:
Exon (11)1154..3372
Gene:PDHA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000284
Organism Homo sapiens (human)
Definition Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000284

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGAGGAAGA TGCTCGCCGC CGTCTCCCGC GTGCTGTCTG GCGCTTCTCA GAAGCCGGCA 
AGCAGAGTGC TGGTAGCATC CCGTAATTTT GCAAATGATG CTACATTTGA AATTAAGAAA
TGTGACCTTC ACCGGCTGGA AGAAGGCCCT CCTGTCACAA CAGTGCTCAC CAGGGAGGAT
GGGCTCAAAT ACTACAGGAT GATGCAGACT GTACGCCGAA TGGAGTTGAA AGCAGATCAG
CTGTATAAAC AGAAAATTAT TCGTGGTTTC TGTCACTTGT GTGATGGTCA GGAAGCTTGC
TGTGTGGGCC TGGAGGCCGG CATCAACCCC ACAGACCATC TCATCACAGC CTACCGGGCT
CACGGCTTTA CTTTCACCCG GGGCCTTTCC GTCCGAGAAA TTCTCGCAGA GCTTACAGGA
CGAAAAGGAG GTTGTGCTAA AGGGAAAGGA GGATCGATGC ACATGTATGC CAAGAACTTC
TACGGGGGCA ATGGCATCGT GGGAGCGCAG GTGCCCCTGG GCGCTGGGAT TGCTCTAGCC
TGTAAGTATA ATGGAAAAGA TGAGGTCTGC CTGACTTTAT ATGGCGATGG TGCTGCTAAC
CAGGGCCAGA TATTCGAAGC TTACAACATG GCAGCTTTGT GGAAATTACC TTGTATTTTC
ATCTGTGAGA ATAATCGCTA TGGAATGGGA ACGTCTGTTG AGAGAGCGGC AGCCAGCACT
GATTACTACA AGAGAGGCGA TTTCATTCCT GGGCTGAGAG TGGATGGAAT GGATATCCTG
TGCGTCCGAG AGGCAACAAG GTTTGCTGCT GCCTATTGTA GATCTGGGAA GGGGCCCATC
CTGATGGAGC TGCAGACTTA CCGTTACCAC GGACACAGTA TGAGTGACCC TGGAGTCAGT
TACCGTACAC GAGAAGAAAT TCAGGAAGTA AGAAGTAAGA GTGACCCTAT TATGCTTCTC
AAGGACAGGA TGGTGAACAG CAATCTTGCC AGTGTGGAAG AACTAAAGGA AATTGATGTG
GAAGTGAGGA AGGAGATTGA GGATGCTGCC CAGTTTGCCA CGGCCGATCC TGAGCCACCT
TTGGAAGAGC TGGGCTACCA CATCTACTCC AGCGACCCAC CTTTTGAAGT TCGTGGTGCC
AATCAGTGGA TCAAGTTTAA GTCAGTCAGT TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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