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PDHB pyruvate dehydrogenase (lipoamide) beta [Homo sapiens (human)]

Gene Symbol PDHB
Entrez Gene ID 5162
Full Name pyruvate dehydrogenase (lipoamide) beta
Synonyms PDHBD, PDHE1-B, PHE1B
General protein information
Preferred Names
pyruvate dehydrogenase E1 component subunit beta, mitochondrial
Names
pyruvate dehydrogenase E1 component subunit beta, mitochondrial
pyruvate dehydrogenase, E1 beta polypeptide
NP_000916.2
NP_001166939.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

3

3p21.1-p14.2

Summary The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and carbon dioxide, and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 beta subunit. Mutations in this gene are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2012]. lac of sum
Disorder MIM:

179060

Disorder Html: Pyruvate dehydrogenase E1-beta deficiency (3)





The following PDHB gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDHB gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu59601 XM_011533828 PREDICTED: Homo sapiens pyruvate dehydrogenase (lipoamide) beta (PDHB), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu18263 NM_000925 Homo sapiens pyruvate dehydrogenase (lipoamide) beta (PDHB), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$265.3
$379
OHu09402 NM_001173468 Homo sapiens pyruvate dehydrogenase (lipoamide) beta (PDHB), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $289

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu59601
Accession Version XM_011533828.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1026bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase E1 component subunit beta, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGGCGG TGTCTGGCTT GGTGCGGAGA CCCCTTCGGG AGGTGACAGT TCGTGATGCT 
ATAAATCAGG GTATGGATGA GGAGCTGGAA AGAGATGAGA AGGTATTTCT GCTTGGAGAA
GAAGTTGCCC AGTATGATGG GGCATACAAG GTTAGTCGAG GGCTGTGGAA GAAATATGGA
GACAAGAGGA TTATTGACAC TCCCATATCA GAGATGGGCT TTGCTGGAAT TGCTGTAGGT
GCAGCTATGG CTGGGTTGCG GCCCATTTGT GAATTTATGA CCTTCAATTT CTCCATGCAA
GCCATTGACC AGGTTATAAA CTCAGCTGCC AAGACCTACT ACATGTCTGG TGGCCTTCAG
CCTGTGCCTA TAGTCTTCAG AGGGCCCAAT GGTGCCTCAG CAGGTGTAGC TGCCCAGCAC
TCACAGTGCT TTGCTGCCTG GTATGGGCAC TGCCCAGGCT TAAAGGTGGT CAGTCCCTGG
AATTCAGAGG ATGCTAAAGG ACTTATTAAA TCAGCCATTC GGGATAACAA TCCAGTGGTG
GTGCTAGAGA ATGAATTGAT GTATGGGGTT CCTTTTGAAT TTCCTCCGGA AGCTCAGTCA
AAAGATTTTC TGATTCCTAT TGGAAAAGCC AAAATAGAAA GGCAAGGAAC ACATATAACT
GTGGTTTCCC ATTCAAGACC TGTGGGCCAC TGCTTAGAAG CTGCAGCAGT GCTATCTAAA
GAAGGAGTTG AATGTGAGGT GATAAATATG CGTACCATTA GACCAATGGA CATGGAAACC
ATAGAAGCCA GTGTCATGAA GACAAATCAT CTTGTAACTG TGGAAGGAGG CTGGCCACAG
TTTGGAGTAG GAGCTGAAAT CTGTGCCAGG ATCATGGAAG GTCCTGCGTT CAATTTCCTG
GATGCTCCTG CTGTTCGTGT CACTGGTGCT GATGTCCCTA TGCCTTATGC AAAGATTCTA
GAGGACAACT CTATACCTCA GGTCAAAGAC ATCATATTTG CAATAAAGAA AACATTAAAT
ATTTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011532130.1
CDS31..1056
Misc Feature(1)67..1044(+)
Misc Feature(2)85..585(+)
Misc Feature(3)127..561(+)
Misc Feature(4)148..330(+)
Misc Feature(5)151..486(+)
Misc Feature(6)652..1020(+)
Translation
Position Chain Variation Link
5 5 a, g dbSNP:201243189
16 16 c, g dbSNP:777786689
19 19 a, g dbSNP:758352130
26 26 c, g dbSNP:367624729
27 27 c, t dbSNP:778465584
28 28 a, g dbSNP:557082585
35 35 c, t dbSNP:753447396
38 38 c, t dbSNP:536872599
40 40 a, g dbSNP:201470762
41 41 c, t dbSNP:751921912
43 43 a, c, t dbSNP:200470565
45 45 c, t dbSNP:775907917
52 52 a, g dbSNP:769880461
54 54 a, g, t dbSNP:776938446
55 55 a, c, t dbSNP:747302644
59 59 a, g dbSNP:777698544
63 63 c, g dbSNP:771813233
64 64 a, c, t dbSNP:778745122
67 67 c, g dbSNP:754460400
68 68 a, g dbSNP:202185395
69 69 a, g dbSNP:779611285
82 82 c, t dbSNP:763842440
91 91 a, g dbSNP:762766442
93 93 a, t dbSNP:775351153
98 98 -, ag dbSNP:778727916
108 108 c, t dbSNP:11542399
114 114 a, g dbSNP:11542403
119 119 a, g dbSNP:759539954
124 124 a, g dbSNP:111470467
126 126 c, t dbSNP:367846961
139 139 c, t dbSNP:11542400
161 161 a, g dbSNP:777514148
166 166 c, g dbSNP:769095344
172 172 a, g dbSNP:749682039
174 174 a, g dbSNP:138621975
179 179 a, g dbSNP:200879699
187 187 c, t dbSNP:373124742
188 188 a, g dbSNP:772939767
190 190 g, t dbSNP:771853453
192 192 a, g dbSNP:150167131
204 204 a, g dbSNP:780552911
257 257 a, g dbSNP:11542401
264 264 c, t dbSNP:138128142
269 269 a, g dbSNP:771466468
277 277 a, g dbSNP:747573712
285 285 a, g, t dbSNP:773743155
286 286 g, t dbSNP:201199166
288 288 g, t dbSNP:748243880
289 289 c, g, t dbSNP:768916456
292 292 c, g dbSNP:749527278
295 295 a, c dbSNP:779872594
299 299 c, g dbSNP:75997072
303 303 a, g dbSNP:370951271
310 310 a, g dbSNP:534238658
317 317 a, g dbSNP:751436063
325 325 a, t dbSNP:781299313
342 342 a, g dbSNP:756767523
366 366 c, t dbSNP:192604927
371 371 a, g dbSNP:28935769
374 374 c, t dbSNP:751111486
391 391 c, t dbSNP:777639555
393 393 c, t dbSNP:762644804
396 396 a, g dbSNP:752332234
400 400 a, g dbSNP:766730807
402 402 a, g dbSNP:151247980
411 411 a, g dbSNP:4264746
414 414 -, a dbSNP:200687616
414 414 a, g dbSNP:1126551
415 415 c, t dbSNP:762371237
416 416 c, g dbSNP:774472788
419 419 a, g dbSNP:768663547
424 424 g, t dbSNP:139242990
434 434 a, g dbSNP:780170320
447 447 a, g dbSNP:769662255
453 453 a, c dbSNP:569429778
468 468 c, t dbSNP:200016955
474 474 c, t dbSNP:757249455
477 477 a, g, t dbSNP:759053641
483 483 c, t dbSNP:143765288
490 490 c, t dbSNP:535839290
496 496 a, g, t dbSNP:760966357
512 512 a, g dbSNP:750784388
535 535 -, a dbSNP:753215190
536 536 c, t dbSNP:767924413
540 540 a, g dbSNP:146996786
559 559 a, g dbSNP:774756909
569 569 c, t dbSNP:35514615
583 583 g, t dbSNP:767832452
611 611 c, t dbSNP:757645498
612 612 -, t dbSNP:766412593
614 614 c, t dbSNP:4071983
617 617 c, t dbSNP:752056463
618 618 a, g, t dbSNP:763021302
622 622 a, g dbSNP:775425923
628 628 a, t dbSNP:765457802
630 630 a, t dbSNP:759802905
634 634 a, g dbSNP:777024340
645 645 c, t dbSNP:573987651
648 648 c, t dbSNP:746797295
649 649 a, g dbSNP:773139051
667 667 c, g dbSNP:771901438
674 674 a, g dbSNP:146343258
685 685 a, g dbSNP:753206504
686 686 c, t dbSNP:779577082
692 692 -, tg dbSNP:772084220
706 706 a, c dbSNP:755045002
718 718 c, t dbSNP:138764163
723 723 c, t dbSNP:766540951
741 741 c, g dbSNP:760789048
743 743 c, t dbSNP:750694855
752 752 a, g dbSNP:767174898
758 758 c, t dbSNP:761614417
766 766 a, g dbSNP:774212597
768 768 a, g dbSNP:768584790
780 780 a, g dbSNP:774836997
782 782 a, g dbSNP:769187141
787 787 a, g dbSNP:755591394
802 802 a, g, t dbSNP:372743787
811 811 a, g dbSNP:756086285
817 817 g, t dbSNP:148957406
828 828 c, g dbSNP:369190855
835 835 a, g dbSNP:202236415
838 838 a, c, t dbSNP:376110096
842 842 c, t dbSNP:371393276
861 861 c, t dbSNP:763844372
868 868 a, c dbSNP:13446
870 870 a, g dbSNP:758213613
872 872 a, t dbSNP:752521945
873 873 c, t dbSNP:145795547
875 875 -, g dbSNP:757230123
883 883 a, g dbSNP:759461639
891 891 a, c dbSNP:140921216
892 892 c, t dbSNP:145876456
893 893 c, g dbSNP:140744404
897 897 c, t dbSNP:760374916
913 913 c, g dbSNP:201105914
917 917 c, t dbSNP:770940287
918 918 a, g, t dbSNP:148770511
932 932 a, t dbSNP:199983136
937 937 c, t dbSNP:772328849
942 942 c, t dbSNP:577477453
947 947 a, g dbSNP:778741789
951 951 c, t dbSNP:754765239
966 966 c, t dbSNP:267599918
967 967 c, t dbSNP:267599917
968 968 c, g dbSNP:753757096
972 972 a, g dbSNP:780150185
978 978 c, t dbSNP:755616546
981 981 a, g dbSNP:749945707
982 982 a, g dbSNP:767192593
983 983 a, g dbSNP:368051123
984 984 a, g dbSNP:751210948
986 986 a, t dbSNP:760143249
998 998 a, g dbSNP:751928661
1001 1001 c, g dbSNP:777056005
1002 1002 c, t dbSNP:771426412
1005 1005 a, g dbSNP:149830613
1006 1006 c, t dbSNP:28933391
1018 1018 g, t dbSNP:773256169
1023 1023 a, c dbSNP:772087134
1033 1033 a, g dbSNP:748366089
1035 1035 a, g dbSNP:537466954
1045 1045 c, t dbSNP:768436316
1049 1049 a, g dbSNP:749094591
1060 1060 a, g dbSNP:780059698
1063 1063 c, g, t dbSNP:374476885
1069 1069 -, c dbSNP:748490971
1078 1078 a, g dbSNP:370072205
1087 1087 c, t dbSNP:547747406
1099 1099 g, t dbSNP:751173808
1101 1101 -, g dbSNP:781681934
1125 1125 c, t dbSNP:763659400
1127 1127 a, g dbSNP:758137448
1128 1128 c, t dbSNP:754190213
1133 1133 c, g dbSNP:766780930
1140 1140 a, g dbSNP:761142168
1142 1142 c, t dbSNP:773743199
1143 1143 c, g dbSNP:767589310
1144 1144 a, g dbSNP:761809361
1148 1148 a, c, t dbSNP:4228
1153 1153 a, c, g dbSNP:555536762
1155 1155 c, g, t dbSNP:7230
1156 1156 a, g dbSNP:745760468
1157 1157 a, g dbSNP:547008035
1168 1168 a, c dbSNP:140169849
1171 1171 a, g dbSNP:780817297
1173 1173 a, c, t dbSNP:746496960
1191 1191 -, g dbSNP:755440612
1207 1207 c, t dbSNP:777401294
1229 1229 c, t dbSNP:546485141
1231 1231 c, t dbSNP:369712686
1257 1257 c, t dbSNP:375277648
1260 1260 c, g dbSNP:146644840
1261 1261 c, t dbSNP:756497263
1267 1267 a, g dbSNP:750876078
1295 1295 a, g dbSNP:767911301
1299 1299 a, c dbSNP:1126722
1309 1309 c, t dbSNP:367907119
1341 1341 a, g dbSNP:530847041
1352 1352 c, g dbSNP:7231
1363 1363 g, t dbSNP:548321877
1379 1379 c, t dbSNP:764281059
1383 1383 a, t dbSNP:763235215
1393 1393 a, g dbSNP:528568497
1406 1406 a, g dbSNP:775884188
1409 1409 a, c, t dbSNP:1126735
1412 1412 a, g dbSNP:1126737
1427 1427 -, gtt dbSNP:752036587
1427 1427 g, t dbSNP:770760887
1431 1431 c, t dbSNP:577439548
1446 1446 a, g dbSNP:747019479

Target ORF information:

RefSeq Version XM_011533828
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pyruvate dehydrogenase (lipoamide) beta (PDHB), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011533828

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGGCGG TGTCTGGCTT GGTGCGGAGA CCCCTTCGGG AGGTGACAGT TCGTGATGCT 
ATAAATCAGG GTATGGATGA GGAGCTGGAA AGAGATGAGA AGGTATTTCT GCTTGGAGAA
GAAGTTGCCC AGTATGATGG GGCATACAAG GTTAGTCGAG GGCTGTGGAA GAAATATGGA
GACAAGAGGA TTATTGACAC TCCCATATCA GAGATGGGCT TTGCTGGAAT TGCTGTAGGT
GCAGCTATGG CTGGGTTGCG GCCCATTTGT GAATTTATGA CCTTCAATTT CTCCATGCAA
GCCATTGACC AGGTTATAAA CTCAGCTGCC AAGACCTACT ACATGTCTGG TGGCCTTCAG
CCTGTGCCTA TAGTCTTCAG AGGGCCCAAT GGTGCCTCAG CAGGTGTAGC TGCCCAGCAC
TCACAGTGCT TTGCTGCCTG GTATGGGCAC TGCCCAGGCT TAAAGGTGGT CAGTCCCTGG
AATTCAGAGG ATGCTAAAGG ACTTATTAAA TCAGCCATTC GGGATAACAA TCCAGTGGTG
GTGCTAGAGA ATGAATTGAT GTATGGGGTT CCTTTTGAAT TTCCTCCGGA AGCTCAGTCA
AAAGATTTTC TGATTCCTAT TGGAAAAGCC AAAATAGAAA GGCAAGGAAC ACATATAACT
GTGGTTTCCC ATTCAAGACC TGTGGGCCAC TGCTTAGAAG CTGCAGCAGT GCTATCTAAA
GAAGGAGTTG AATGTGAGGT GATAAATATG CGTACCATTA GACCAATGGA CATGGAAACC
ATAGAAGCCA GTGTCATGAA GACAAATCAT CTTGTAACTG TGGAAGGAGG CTGGCCACAG
TTTGGAGTAG GAGCTGAAAT CTGTGCCAGG ATCATGGAAG GTCCTGCGTT CAATTTCCTG
GATGCTCCTG CTGTTCGTGT CACTGGTGCT GATGTCCCTA TGCCTTATGC AAAGATTCTA
GAGGACAACT CTATACCTCA GGTCAAAGAC ATCATATTTG CAATAAAGAA AACATTAAAT
ATTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18263
Accession Version NM_000925.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1080bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase E1 component subunit beta, mitochondrial isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA965753.1, AK225569.1, AK293153.1 and BM971427.1. This sequence is a reference standard in the RefSeqGene project. On Mar 16, 2010 this sequence version replaced gi:156564402. Summary: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and carbon dioxide, and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 beta subunit. Mutations in this gene are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2012]. Transcript Variant: This variant (1) represents the predominant transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK225621.1, J03576.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGGCGG TGTCTGGCTT GGTGCGGAGA CCCCTTCGGG AGGTCTCCGG GCTGCTGAAG 
AGGCGCTTTC ACTGGACCGC GCCGGCTGCG CTGCAGGTGA CAGTTCGTGA TGCTATAAAT
CAGGGTATGG ATGAGGAGCT GGAAAGAGAT GAGAAGGTAT TTCTGCTTGG AGAAGAAGTT
GCCCAGTATG ATGGGGCATA CAAGGTTAGT CGAGGGCTGT GGAAGAAATA TGGAGACAAG
AGGATTATTG ACACTCCCAT ATCAGAGATG GGCTTTGCTG GAATTGCTGT AGGTGCAGCT
ATGGCTGGGT TGCGGCCCAT TTGTGAATTT ATGACCTTCA ATTTCTCCAT GCAAGCCATT
GACCAGGTTA TAAACTCAGC TGCCAAGACC TACTACATGT CTGGTGGCCT TCAGCCTGTG
CCTATAGTCT TCAGGGGGCC CAATGGTGCC TCAGCAGGTG TAGCTGCCCA GCACTCACAG
TGCTTTGCTG CCTGGTATGG GCACTGCCCA GGCTTAAAGG TGGTCAGTCC CTGGAATTCA
GAGGATGCTA AAGGACTTAT TAAATCAGCC ATTCGGGATA ACAATCCAGT GGTGGTGCTA
GAGAATGAAT TGATGTATGG GGTTCCTTTT GAATTTCCTC CGGAAGCTCA GTCAAAAGAT
TTTCTGATTC CTATTGGAAA AGCCAAAATA GAAAGGCAAG GAACACATAT AACTGTGGTT
TCCCATTCAA GACCTGTGGG CCACTGCTTA GAAGCTGCAG CAGTGCTATC TAAAGAAGGA
GTTGAATGTG AGGTGATAAA TATGCGTACC ATTAGACCAA TGGACATGGA AACCATAGAA
GCCAGTGTCA TGAAGACAAA TCATCTTGTA ACTGTGGAAG GAGGCTGGCC ACAGTTTGGA
GTAGGAGCTG AAATCTGTGC CAGGATCATG GAAGGTCCTG CGTTCAATTT CCTGGATGCT
CCTGCTGTTC GTGTCACTGG TGCTGATGTC CCTATGCCTT ATGCAAAGAT TCTAGAGGAC
AACTCTATAC CTCAGGTCAA AGACATCATA TTTGCAATAA AGAAAACATT AAATATTTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000916.2
CDS44..1123
Misc Feature(1)59..1111(+)
Misc Feature(2)152..652(+)
Misc Feature(3)194..628(+)
Misc Feature(4)215..397(+)
Misc Feature(5)218..553(+)
Misc Feature(6)719..1087(+)
Exon (1)1..85
Gene:PDHB
Gene Synonym:
Exon (2)86..139
Gene:PDHB
Gene Synonym:
Exon (3)140..247
Gene:PDHB
Gene Synonym:
Exon (4)248..310
Gene:PDHB
Gene Synonym:
Exon (5)311..346
Gene:PDHB
Gene Synonym:
Exon (6)347..632
Gene:PDHB
Gene Synonym:
Exon (7)633..743
Gene:PDHB
Gene Synonym:
Exon (8)744..835
Gene:PDHB
Gene Synonym:
Exon (9)836..977
Gene:PDHB
Gene Synonym:
Exon (10)978..1527
Gene:PDHB
Gene Synonym:
Translation
Position Chain Variation Link
2 2 a, c, t dbSNP:762870333
3 3 c, t dbSNP:775631842
4 4 a, c, t dbSNP:539491929
5 5 c, t dbSNP:781599347
12 12 c, t dbSNP:770804747
18 18 a, g dbSNP:201243189
29 29 c, g dbSNP:777786689
32 32 a, g dbSNP:758352130
39 39 c, g dbSNP:367624729
40 40 c, t dbSNP:778465584
41 41 a, g dbSNP:557082585
48 48 c, t dbSNP:753447396
51 51 c, t dbSNP:536872599
53 53 a, g dbSNP:201470762
54 54 c, t dbSNP:751921912
56 56 a, c, t dbSNP:200470565
58 58 c, t dbSNP:775907917
65 65 a, g dbSNP:769880461
67 67 a, g, t dbSNP:776938446
68 68 a, c, t dbSNP:747302644
72 72 a, g dbSNP:777698544
76 76 c, g dbSNP:771813233
77 77 a, c, t dbSNP:778745122
80 80 c, g dbSNP:754460400
81 81 a, g dbSNP:202185395
82 82 a, g dbSNP:779611285
88 88 c, g dbSNP:747916943
91 91 c, t dbSNP:375651141
95 95 c, t dbSNP:534688111
101 101 a, g dbSNP:749285624
105 105 c, g dbSNP:565786614
121 121 c, t dbSNP:552159384
124 124 a, g dbSNP:745487481
129 129 c, t dbSNP:780733289
131 131 a, g dbSNP:758735231
149 149 c, t dbSNP:763842440
158 158 a, g dbSNP:762766442
160 160 a, t dbSNP:775351153
165 165 -, ag dbSNP:778727916
175 175 c, t dbSNP:11542399
181 181 a, g dbSNP:11542403
186 186 a, g dbSNP:759539954
191 191 a, g dbSNP:111470467
193 193 c, t dbSNP:367846961
206 206 c, t dbSNP:11542400
228 228 a, g dbSNP:777514148
233 233 c, g dbSNP:769095344
239 239 a, g dbSNP:749682039
241 241 a, g dbSNP:138621975
246 246 a, g dbSNP:200879699
254 254 c, t dbSNP:373124742
255 255 a, g dbSNP:772939767
257 257 g, t dbSNP:771853453
259 259 a, g dbSNP:150167131
271 271 a, g dbSNP:780552911
324 324 a, g dbSNP:11542401
331 331 c, t dbSNP:138128142
336 336 a, g dbSNP:771466468
344 344 a, g dbSNP:747573712
352 352 a, g, t dbSNP:773743155
353 353 g, t dbSNP:201199166
355 355 g, t dbSNP:748243880
356 356 c, g, t dbSNP:768916456
359 359 c, g dbSNP:749527278
362 362 a, c dbSNP:779872594
366 366 c, g dbSNP:75997072
370 370 a, g dbSNP:370951271
377 377 a, g dbSNP:534238658
384 384 a, g dbSNP:751436063
392 392 a, t dbSNP:781299313
409 409 a, g dbSNP:756767523
433 433 c, t dbSNP:192604927
438 438 a, g dbSNP:28935769
441 441 c, t dbSNP:751111486
458 458 c, t dbSNP:777639555
460 460 c, t dbSNP:762644804
463 463 a, g dbSNP:752332234
467 467 a, g dbSNP:766730807
469 469 a, g dbSNP:151247980
478 478 a, g dbSNP:4264746
481 481 -, a dbSNP:200687616
481 481 a, g dbSNP:1126551
482 482 c, t dbSNP:762371237
483 483 c, g dbSNP:774472788
486 486 a, g dbSNP:768663547
491 491 g, t dbSNP:139242990
501 501 a, g dbSNP:780170320
514 514 a, g dbSNP:769662255
520 520 a, c dbSNP:569429778
535 535 c, t dbSNP:200016955
541 541 c, t dbSNP:757249455
544 544 a, g, t dbSNP:759053641
550 550 c, t dbSNP:143765288
557 557 c, t dbSNP:535839290
563 563 a, g, t dbSNP:760966357
579 579 a, g dbSNP:750784388
602 602 -, a dbSNP:753215190
603 603 c, t dbSNP:767924413
607 607 a, g dbSNP:146996786
626 626 a, g dbSNP:774756909
636 636 c, t dbSNP:35514615
650 650 g, t dbSNP:767832452
678 678 c, t dbSNP:757645498
679 679 -, t dbSNP:766412593
681 681 c, t dbSNP:4071983
684 684 c, t dbSNP:752056463
685 685 a, g, t dbSNP:763021302
689 689 a, g dbSNP:775425923
695 695 a, t dbSNP:765457802
697 697 a, t dbSNP:759802905
701 701 a, g dbSNP:777024340
712 712 c, t dbSNP:573987651
715 715 c, t dbSNP:746797295
716 716 a, g dbSNP:773139051
734 734 c, g dbSNP:771901438
741 741 a, g dbSNP:146343258
752 752 a, g dbSNP:753206504
753 753 c, t dbSNP:779577082
759 759 -, tg dbSNP:772084220
773 773 a, c dbSNP:755045002
785 785 c, t dbSNP:138764163
790 790 c, t dbSNP:766540951
808 808 c, g dbSNP:760789048
810 810 c, t dbSNP:750694855
819 819 a, g dbSNP:767174898
825 825 c, t dbSNP:761614417
833 833 a, g dbSNP:774212597
835 835 a, g dbSNP:768584790
847 847 a, g dbSNP:774836997
849 849 a, g dbSNP:769187141
854 854 a, g dbSNP:755591394
869 869 a, g, t dbSNP:372743787
878 878 a, g dbSNP:756086285
884 884 g, t dbSNP:148957406
895 895 c, g dbSNP:369190855
902 902 a, g dbSNP:202236415
905 905 a, c, t dbSNP:376110096
909 909 c, t dbSNP:371393276
928 928 c, t dbSNP:763844372
935 935 a, c dbSNP:13446
937 937 a, g dbSNP:758213613
939 939 a, t dbSNP:752521945
940 940 c, t dbSNP:145795547
942 942 -, g dbSNP:757230123
950 950 a, g dbSNP:759461639
958 958 a, c dbSNP:140921216
959 959 c, t dbSNP:145876456
960 960 c, g dbSNP:140744404
964 964 c, t dbSNP:760374916
980 980 c, g dbSNP:201105914
984 984 c, t dbSNP:770940287
985 985 a, g, t dbSNP:148770511
999 999 a, t dbSNP:199983136
1004 1004 c, t dbSNP:772328849
1009 1009 c, t dbSNP:577477453
1014 1014 a, g dbSNP:778741789
1018 1018 c, t dbSNP:754765239
1033 1033 c, t dbSNP:267599918
1034 1034 c, t dbSNP:267599917
1035 1035 c, g dbSNP:753757096
1039 1039 a, g dbSNP:780150185
1045 1045 c, t dbSNP:755616546
1048 1048 a, g dbSNP:749945707
1049 1049 a, g dbSNP:767192593
1050 1050 a, g dbSNP:368051123
1051 1051 a, g dbSNP:751210948
1053 1053 a, t dbSNP:760143249
1065 1065 a, g dbSNP:751928661
1068 1068 c, g dbSNP:777056005
1069 1069 c, t dbSNP:771426412
1072 1072 a, g dbSNP:149830613
1073 1073 c, t dbSNP:28933391
1085 1085 g, t dbSNP:773256169
1090 1090 a, c dbSNP:772087134
1100 1100 a, g dbSNP:748366089
1102 1102 a, g dbSNP:537466954
1112 1112 c, t dbSNP:768436316
1116 1116 a, g dbSNP:749094591
1127 1127 a, g dbSNP:780059698
1130 1130 c, g, t dbSNP:374476885
1136 1136 -, c dbSNP:748490971
1145 1145 a, g dbSNP:370072205
1154 1154 c, t dbSNP:547747406
1166 1166 g, t dbSNP:751173808
1168 1168 -, g dbSNP:781681934
1192 1192 c, t dbSNP:763659400
1194 1194 a, g dbSNP:758137448
1195 1195 c, t dbSNP:754190213
1200 1200 c, g dbSNP:766780930
1207 1207 a, g dbSNP:761142168
1209 1209 c, t dbSNP:773743199
1210 1210 c, g dbSNP:767589310
1211 1211 a, g dbSNP:761809361
1215 1215 a, c, t dbSNP:4228
1220 1220 a, c, g dbSNP:555536762
1222 1222 c, g, t dbSNP:7230
1223 1223 a, g dbSNP:745760468
1224 1224 a, g dbSNP:547008035
1235 1235 a, c dbSNP:140169849
1238 1238 a, g dbSNP:780817297
1240 1240 a, c, t dbSNP:746496960
1258 1258 -, g dbSNP:755440612
1274 1274 c, t dbSNP:777401294
1296 1296 c, t dbSNP:546485141
1298 1298 c, t dbSNP:369712686
1324 1324 c, t dbSNP:375277648
1327 1327 c, g dbSNP:146644840
1328 1328 c, t dbSNP:756497263
1334 1334 a, g dbSNP:750876078
1362 1362 a, g dbSNP:767911301
1366 1366 a, c dbSNP:1126722
1376 1376 c, t dbSNP:367907119
1408 1408 a, g dbSNP:530847041
1419 1419 c, g dbSNP:7231
1430 1430 g, t dbSNP:548321877
1446 1446 c, t dbSNP:764281059
1450 1450 a, t dbSNP:763235215
1460 1460 a, g dbSNP:528568497
1473 1473 a, g dbSNP:775884188
1476 1476 a, c, t dbSNP:1126735
1479 1479 a, g dbSNP:1126737
1494 1494 -, gtt dbSNP:752036587
1494 1494 g, t dbSNP:770760887
1498 1498 c, t dbSNP:577439548
1513 1513 a, g dbSNP:747019479

Target ORF information:

RefSeq Version NM_000925
Organism Homo sapiens (human)
Definition Homo sapiens pyruvate dehydrogenase (lipoamide) beta (PDHB), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000925

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGGCGG TGTCTGGCTT GGTGCGGAGA CCCCTTCGGG AGGTCTCCGG GCTGCTGAAG 
AGGCGCTTTC ACTGGACCGC GCCGGCTGCG CTGCAGGTGA CAGTTCGTGA TGCTATAAAT
CAGGGTATGG ATGAGGAGCT GGAAAGAGAT GAGAAGGTAT TTCTGCTTGG AGAAGAAGTT
GCCCAGTATG ATGGGGCATA CAAGGTTAGT CGAGGGCTGT GGAAGAAATA TGGAGACAAG
AGGATTATTG ACACTCCCAT ATCAGAGATG GGCTTTGCTG GAATTGCTGT AGGTGCAGCT
ATGGCTGGGT TGCGGCCCAT TTGTGAATTT ATGACCTTCA ATTTCTCCAT GCAAGCCATT
GACCAGGTTA TAAACTCAGC TGCCAAGACC TACTACATGT CTGGTGGCCT TCAGCCTGTG
CCTATAGTCT TCAGGGGGCC CAATGGTGCC TCAGCAGGTG TAGCTGCCCA GCACTCACAG
TGCTTTGCTG CCTGGTATGG GCACTGCCCA GGCTTAAAGG TGGTCAGTCC CTGGAATTCA
GAGGATGCTA AAGGACTTAT TAAATCAGCC ATTCGGGATA ACAATCCAGT GGTGGTGCTA
GAGAATGAAT TGATGTATGG GGTTCCTTTT GAATTTCCTC CGGAAGCTCA GTCAAAAGAT
TTTCTGATTC CTATTGGAAA AGCCAAAATA GAAAGGCAAG GAACACATAT AACTGTGGTT
TCCCATTCAA GACCTGTGGG CCACTGCTTA GAAGCTGCAG CAGTGCTATC TAAAGAAGGA
GTTGAATGTG AGGTGATAAA TATGCGTACC ATTAGACCAA TGGACATGGA AACCATAGAA
GCCAGTGTCA TGAAGACAAA TCATCTTGTA ACTGTGGAAG GAGGCTGGCC ACAGTTTGGA
GTAGGAGCTG AAATCTGTGC CAGGATCATG GAAGGTCCTG CGTTCAATTT CCTGGATGCT
CCTGCTGTTC GTGTCACTGG TGCTGATGTC CCTATGCCTT ATGCAAAGAT TCTAGAGGAC
AACTCTATAC CTCAGGTCAA AGACATCATA TTTGCAATAA AGAAAACATT AAATATTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09402
Accession Version NM_001173468.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1026bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase E1 component subunit beta, mitochondrial isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA965753.1, AK293153.1 and BM971427.1. Summary: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and carbon dioxide, and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 beta subunit. Mutations in this gene are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2012]. Transcript Variant: This variant (2) lacks a segment in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK293153.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1970526, SAMEA2146411 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGGCGG TGTCTGGCTT GGTGCGGAGA CCCCTTCGGG AGGTCTCCGG GCTGCTGAAG 
AGGCGCTTTC ACTGGACCGC GCCGGCTGCG CTGCAGGTGA CAGTTCGTGA TGCTATAAAT
CAGGGTATGG ATGAGGAGCT GGAAAGAGAT GAGAAGGTAT TTCTGCTTGG AGAAGAAGTT
GCCCAGTATG ATGGGGCATA CAAGGTTAGT CGAGGGCTGT GGAAGAAATA TGGAGACAAG
AGGATTATTG ACACTCCCAT ATCAGAGATG GGCTTTGCTG GAATTGCTGT AGGTGCAGCT
ATGGCTGGGT TGCGGCCCAT TTGTGAATTT ATGACCTTCA ATTTCTCCAT GCAAGCCATT
GACCAGGTTA TAAACTCAGC TGCCAAGACC TACTACATGT CTGGTGTAGC TGCCCAGCAC
TCACAGTGCT TTGCTGCCTG GTATGGGCAC TGCCCAGGCT TAAAGGTGGT CAGTCCCTGG
AATTCAGAGG ATGCTAAAGG ACTTATTAAA TCAGCCATTC GGGATAACAA TCCAGTGGTG
GTGCTAGAGA ATGAATTGAT GTATGGGGTT CCTTTTGAAT TTCCTCCGGA AGCTCAGTCA
AAAGATTTTC TGATTCCTAT TGGAAAAGCC AAAATAGAAA GGCAAGGAAC ACATATAACT
GTGGTTTCCC ATTCAAGACC TGTGGGCCAC TGCTTAGAAG CTGCAGCAGT GCTATCTAAA
GAAGGAGTTG AATGTGAGGT GATAAATATG CGTACCATTA GACCAATGGA CATGGAAACC
ATAGAAGCCA GTGTCATGAA GACAAATCAT CTTGTAACTG TGGAAGGAGG CTGGCCACAG
TTTGGAGTAG GAGCTGAAAT CTGTGCCAGG ATCATGGAAG GTCCTGCGTT CAATTTCCTG
GATGCTCCTG CTGTTCGTGT CACTGGTGCT GATGTCCCTA TGCCTTATGC AAAGATTCTA
GAGGACAACT CTATACCTCA GGTCAAAGAC ATCATATTTG CAATAAAGAA AACATTAAAT
ATTTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001166939.1
CDS44..1069
Misc Feature(1)59..1057(+)
Misc Feature(2)152..598(+)
Misc Feature(3)194..574(+)
Misc Feature(4)215..397(+)
Misc Feature(5)218..499(+)
Misc Feature(6)665..1033(+)
Exon (1)1..85
Gene:PDHB
Gene Synonym:
Exon (2)86..139
Gene:PDHB
Gene Synonym:
Exon (3)140..247
Gene:PDHB
Gene Synonym:
Exon (4)248..310
Gene:PDHB
Gene Synonym:
Exon (5)311..346
Gene:PDHB
Gene Synonym:
Exon (6)347..446
Gene:PDHB
Gene Synonym:
Exon (7)447..578
Gene:PDHB
Gene Synonym:
Exon (8)579..689
Gene:PDHB
Gene Synonym:
Exon (9)690..781
Gene:PDHB
Gene Synonym:
Exon (10)782..923
Gene:PDHB
Gene Synonym:
Exon (11)924..1473
Gene:PDHB
Gene Synonym:
Translation
Position Chain Variation Link
2 2 a, c, t dbSNP:762870333
3 3 c, t dbSNP:775631842
4 4 a, c, t dbSNP:539491929
5 5 c, t dbSNP:781599347
12 12 c, t dbSNP:770804747
18 18 a, g dbSNP:201243189
29 29 c, g dbSNP:777786689
32 32 a, g dbSNP:758352130
39 39 c, g dbSNP:367624729
40 40 c, t dbSNP:778465584
41 41 a, g dbSNP:557082585
48 48 c, t dbSNP:753447396
51 51 c, t dbSNP:536872599
53 53 a, g dbSNP:201470762
54 54 c, t dbSNP:751921912
56 56 a, c, t dbSNP:200470565
58 58 c, t dbSNP:775907917
65 65 a, g dbSNP:769880461
67 67 a, g, t dbSNP:776938446
68 68 a, c, t dbSNP:747302644
72 72 a, g dbSNP:777698544
76 76 c, g dbSNP:771813233
77 77 a, c, t dbSNP:778745122
80 80 c, g dbSNP:754460400
81 81 a, g dbSNP:202185395
82 82 a, g dbSNP:779611285
88 88 c, g dbSNP:747916943
91 91 c, t dbSNP:375651141
95 95 c, t dbSNP:534688111
101 101 a, g dbSNP:749285624
105 105 c, g dbSNP:565786614
121 121 c, t dbSNP:552159384
124 124 a, g dbSNP:745487481
129 129 c, t dbSNP:780733289
131 131 a, g dbSNP:758735231
149 149 c, t dbSNP:763842440
158 158 a, g dbSNP:762766442
160 160 a, t dbSNP:775351153
165 165 -, ag dbSNP:778727916
175 175 c, t dbSNP:11542399
181 181 a, g dbSNP:11542403
186 186 a, g dbSNP:759539954
191 191 a, g dbSNP:111470467
193 193 c, t dbSNP:367846961
206 206 c, t dbSNP:11542400
228 228 a, g dbSNP:777514148
233 233 c, g dbSNP:769095344
239 239 a, g dbSNP:749682039
241 241 a, g dbSNP:138621975
246 246 a, g dbSNP:200879699
254 254 c, t dbSNP:373124742
255 255 a, g dbSNP:772939767
257 257 g, t dbSNP:771853453
259 259 a, g dbSNP:150167131
271 271 a, g dbSNP:780552911
324 324 a, g dbSNP:11542401
331 331 c, t dbSNP:138128142
336 336 a, g dbSNP:771466468
344 344 a, g dbSNP:747573712
352 352 a, g, t dbSNP:773743155
353 353 g, t dbSNP:201199166
355 355 g, t dbSNP:748243880
356 356 c, g, t dbSNP:768916456
359 359 c, g dbSNP:749527278
362 362 a, c dbSNP:779872594
366 366 c, g dbSNP:75997072
370 370 a, g dbSNP:370951271
377 377 a, g dbSNP:534238658
384 384 a, g dbSNP:751436063
392 392 a, t dbSNP:781299313
409 409 a, g dbSNP:756767523
433 433 c, t dbSNP:192604927
438 438 a, g dbSNP:28935769
441 441 c, t dbSNP:751111486
447 447 a, g dbSNP:780170320
460 460 a, g dbSNP:769662255
466 466 a, c dbSNP:569429778
481 481 c, t dbSNP:200016955
487 487 c, t dbSNP:757249455
490 490 a, g, t dbSNP:759053641
496 496 c, t dbSNP:143765288
503 503 c, t dbSNP:535839290
509 509 a, g, t dbSNP:760966357
525 525 a, g dbSNP:750784388
548 548 -, a dbSNP:753215190
549 549 c, t dbSNP:767924413
553 553 a, g dbSNP:146996786
572 572 a, g dbSNP:774756909
582 582 c, t dbSNP:35514615
596 596 g, t dbSNP:767832452
624 624 c, t dbSNP:757645498
625 625 -, t dbSNP:766412593
627 627 c, t dbSNP:4071983
630 630 c, t dbSNP:752056463
631 631 a, g, t dbSNP:763021302
635 635 a, g dbSNP:775425923
641 641 a, t dbSNP:765457802
643 643 a, t dbSNP:759802905
647 647 a, g dbSNP:777024340
658 658 c, t dbSNP:573987651
661 661 c, t dbSNP:746797295
662 662 a, g dbSNP:773139051
680 680 c, g dbSNP:771901438
687 687 a, g dbSNP:146343258
698 698 a, g dbSNP:753206504
699 699 c, t dbSNP:779577082
705 705 -, tg dbSNP:772084220
719 719 a, c dbSNP:755045002
731 731 c, t dbSNP:138764163
736 736 c, t dbSNP:766540951
754 754 c, g dbSNP:760789048
756 756 c, t dbSNP:750694855
765 765 a, g dbSNP:767174898
771 771 c, t dbSNP:761614417
779 779 a, g dbSNP:774212597
781 781 a, g dbSNP:768584790
793 793 a, g dbSNP:774836997
795 795 a, g dbSNP:769187141
800 800 a, g dbSNP:755591394
815 815 a, g, t dbSNP:372743787
824 824 a, g dbSNP:756086285
830 830 g, t dbSNP:148957406
841 841 c, g dbSNP:369190855
848 848 a, g dbSNP:202236415
851 851 a, c, t dbSNP:376110096
855 855 c, t dbSNP:371393276
874 874 c, t dbSNP:763844372
881 881 a, c dbSNP:13446
883 883 a, g dbSNP:758213613
885 885 a, t dbSNP:752521945
886 886 c, t dbSNP:145795547
888 888 -, g dbSNP:757230123
896 896 a, g dbSNP:759461639
904 904 a, c dbSNP:140921216
905 905 c, t dbSNP:145876456
906 906 c, g dbSNP:140744404
910 910 c, t dbSNP:760374916
926 926 c, g dbSNP:201105914
930 930 c, t dbSNP:770940287
931 931 a, g, t dbSNP:148770511
945 945 a, t dbSNP:199983136
950 950 c, t dbSNP:772328849
955 955 c, t dbSNP:577477453
960 960 a, g dbSNP:778741789
964 964 c, t dbSNP:754765239
979 979 c, t dbSNP:267599918
980 980 c, t dbSNP:267599917
981 981 c, g dbSNP:753757096
985 985 a, g dbSNP:780150185
991 991 c, t dbSNP:755616546
994 994 a, g dbSNP:749945707
995 995 a, g dbSNP:767192593
996 996 a, g dbSNP:368051123
997 997 a, g dbSNP:751210948
999 999 a, t dbSNP:760143249
1011 1011 a, g dbSNP:751928661
1014 1014 c, g dbSNP:777056005
1015 1015 c, t dbSNP:771426412
1018 1018 a, g dbSNP:149830613
1019 1019 c, t dbSNP:28933391
1031 1031 g, t dbSNP:773256169
1036 1036 a, c dbSNP:772087134
1046 1046 a, g dbSNP:748366089
1048 1048 a, g dbSNP:537466954
1058 1058 c, t dbSNP:768436316
1062 1062 a, g dbSNP:749094591
1073 1073 a, g dbSNP:780059698
1076 1076 c, g, t dbSNP:374476885
1082 1082 -, c dbSNP:748490971
1091 1091 a, g dbSNP:370072205
1100 1100 c, t dbSNP:547747406
1112 1112 g, t dbSNP:751173808
1114 1114 -, g dbSNP:781681934
1138 1138 c, t dbSNP:763659400
1140 1140 a, g dbSNP:758137448
1141 1141 c, t dbSNP:754190213
1146 1146 c, g dbSNP:766780930
1153 1153 a, g dbSNP:761142168
1155 1155 c, t dbSNP:773743199
1156 1156 c, g dbSNP:767589310
1157 1157 a, g dbSNP:761809361
1161 1161 a, c, t dbSNP:4228
1166 1166 a, c, g dbSNP:555536762
1168 1168 c, g, t dbSNP:7230
1169 1169 a, g dbSNP:745760468
1170 1170 a, g dbSNP:547008035
1181 1181 a, c dbSNP:140169849
1184 1184 a, g dbSNP:780817297
1186 1186 a, c, t dbSNP:746496960
1204 1204 -, g dbSNP:755440612
1220 1220 c, t dbSNP:777401294
1242 1242 c, t dbSNP:546485141
1244 1244 c, t dbSNP:369712686
1270 1270 c, t dbSNP:375277648
1273 1273 c, g dbSNP:146644840
1274 1274 c, t dbSNP:756497263
1280 1280 a, g dbSNP:750876078
1308 1308 a, g dbSNP:767911301
1312 1312 a, c dbSNP:1126722
1322 1322 c, t dbSNP:367907119
1354 1354 a, g dbSNP:530847041
1365 1365 c, g dbSNP:7231
1376 1376 g, t dbSNP:548321877
1392 1392 c, t dbSNP:764281059
1396 1396 a, t dbSNP:763235215
1406 1406 a, g dbSNP:528568497
1419 1419 a, g dbSNP:775884188
1422 1422 a, c, t dbSNP:1126735
1425 1425 a, g dbSNP:1126737
1440 1440 -, gtt dbSNP:752036587
1440 1440 g, t dbSNP:770760887
1444 1444 c, t dbSNP:577439548
1459 1459 a, g dbSNP:747019479

Target ORF information:

RefSeq Version NM_001173468
Organism Homo sapiens (human)
Definition Homo sapiens pyruvate dehydrogenase (lipoamide) beta (PDHB), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001173468

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGGCGG TGTCTGGCTT GGTGCGGAGA CCCCTTCGGG AGGTCTCCGG GCTGCTGAAG 
AGGCGCTTTC ACTGGACCGC GCCGGCTGCG CTGCAGGTGA CAGTTCGTGA TGCTATAAAT
CAGGGTATGG ATGAGGAGCT GGAAAGAGAT GAGAAGGTAT TTCTGCTTGG AGAAGAAGTT
GCCCAGTATG ATGGGGCATA CAAGGTTAGT CGAGGGCTGT GGAAGAAATA TGGAGACAAG
AGGATTATTG ACACTCCCAT ATCAGAGATG GGCTTTGCTG GAATTGCTGT AGGTGCAGCT
ATGGCTGGGT TGCGGCCCAT TTGTGAATTT ATGACCTTCA ATTTCTCCAT GCAAGCCATT
GACCAGGTTA TAAACTCAGC TGCCAAGACC TACTACATGT CTGGTGTAGC TGCCCAGCAC
TCACAGTGCT TTGCTGCCTG GTATGGGCAC TGCCCAGGCT TAAAGGTGGT CAGTCCCTGG
AATTCAGAGG ATGCTAAAGG ACTTATTAAA TCAGCCATTC GGGATAACAA TCCAGTGGTG
GTGCTAGAGA ATGAATTGAT GTATGGGGTT CCTTTTGAAT TTCCTCCGGA AGCTCAGTCA
AAAGATTTTC TGATTCCTAT TGGAAAAGCC AAAATAGAAA GGCAAGGAAC ACATATAACT
GTGGTTTCCC ATTCAAGACC TGTGGGCCAC TGCTTAGAAG CTGCAGCAGT GCTATCTAAA
GAAGGAGTTG AATGTGAGGT GATAAATATG CGTACCATTA GACCAATGGA CATGGAAACC
ATAGAAGCCA GTGTCATGAA GACAAATCAT CTTGTAACTG TGGAAGGAGG CTGGCCACAG
TTTGGAGTAG GAGCTGAAAT CTGTGCCAGG ATCATGGAAG GTCCTGCGTT CAATTTCCTG
GATGCTCCTG CTGTTCGTGT CACTGGTGCT GATGTCCCTA TGCCTTATGC AAAGATTCTA
GAGGACAACT CTATACCTCA GGTCAAAGAC ATCATATTTG CAATAAAGAA AACATTAAAT
ATTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.