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PDPK1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol PDPK1
Entrez Gene ID 5170
Full Name 3-phosphoinositide dependent protein kinase 1
Synonyms PDK1, PDPK2, PDPK2P, PRO0461
General protein information
Preferred Names
3-phosphoinositide-dependent protein kinase 1
Names
3-phosphoinositide-dependent protein kinase 1
PkB-like 1
PkB kinase like gene 1
NP_001248745.1
NP_002604.1
NP_112558.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

16

16p13.3

Summary lac of sum
Disorder MIM:

605213


mRNA and Protein(s)

mRNA Protein Name
XM_011522521 XP_011520823 3-phosphoinositide-dependent protein kinase 1 isoform X1
XM_011522522 XP_011520824 3-phosphoinositide-dependent protein kinase 1 isoform X2
XM_005255356 XP_005255413 3-phosphoinositide-dependent protein kinase 1 isoform X3
XM_011522523 XP_011520825 3-phosphoinositide-dependent protein kinase 1 isoform X4
XM_011522524 XP_011520826 3-phosphoinositide-dependent protein kinase 1 isoform X5
NM_002613 NP_002604 3-phosphoinositide-dependent protein kinase 1 isoform 1
NM_031268 NP_112558 3-phosphoinositide-dependent protein kinase 1 isoform 2
NM_001261816 NP_001248745 3-phosphoinositide-dependent protein kinase 1 isoform 3



Homo sapiens (human) PDPK1 NP_002604.1
Pan troglodytes (chimpanzee) PDPK1 XP_003952817.1
Canis lupus familiaris (dog) PDPK1 XP_005621736.1
Bos taurus (cattle) PDPK1 XP_002697908.2
Mus musculus (house mouse) Pdpk1 NP_035192.2
Rattus norvegicus (Norway rat) Pdpk1 NP_112343.1
Gallus gallus (chicken) PDPK1 NP_001012547.1
Danio rerio (zebrafish) pdpk1b NP_991262.1
Drosophila melanogaster (fruit fly) Pdk1 NP_728473.1
Caenorhabditis elegans pdk-1 NP_001024742.1
Arabidopsis thaliana (thale cress) PDK1 NP_568138.1
Arabidopsis thaliana (thale cress) AT3G10540 NP_187665.2
Xenopus (Silurana) tropicalis (western clawed frog) pdpk1 XP_002938460.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PDPK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDPK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu59606 XM_011522521 PREDICTED: Homo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu59607 XM_011522522 PREDICTED: Homo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu48855 XM_005255356 PREDICTED: Homo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $307.30
$439.00
OHu59608 XM_011522523 PREDICTED: Homo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu59609 XM_011522524 PREDICTED: Homo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu13008
NM_002613 Homo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$234.50-$328.30
$469.00
OHu13009
NM_031268 Homo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu13007 NM_001261816 Homo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu59606
Clone ID Related Accession (Same CDS sequence) XM_011522521
Accession Version XM_011522521.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1773bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 3-phosphoinositide-dependent protein kinase 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010393.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGTTGAAGA TGTGGGTTAG TGAAATTGAG ACCTGGCGCT TCCTGAGGCT GGCAGCCCGG 
TTGAGCCTGA GGACCAGACT TGAGGCAGTT GGCAGTCCGC GCCTCGTTGC CCTCTGTCTG
CCCTGGTATG ACGCCGTGCC CATCCAGTCC AGCGTGGTGT TATGTTCCTG CCCATCCCCA
TCAATGGTGA GGACCCAGAC TGAGTCCAGC ACGCCCCCTG GCATTCCTGG TGGCAGCAGG
CAGGGCCCCG CCATGGACGG CACTGCAGCC GAGCCTCGGC CCGGCGCCGG CTCCCTGCAG
CATGCCCAGC CTCCGCCGCA GCCTCGGAAG AAGCGGCCTG AGGACTTCAA GTTTGGGAAA
ATCCTTGGGG AAGGCTCTTT TTCCACGGTT GTCCTGGCTC GAGAACTGGC AACCTCCAGA
GAATATGCGA TTAAAATTCT GGAGAAGCGA CATATCATAA AAGAGAACAA GGTCCCCTAT
GTAACCAGAG AGCGGGATGT CATGTCGCGC CTGGATCACC CCTTCTTTGT TAAGCTTTAC
TTCACATTTC AGGACGACGA GAAGCTGTAT TTCGGCCTTA GTTATGCCAA AAATGGAGAA
CTACTTAAAT ATATTCGCAA AATCGGTTCA TTCGATGAGA CCTGTACCCG ATTTTACACG
GCTGAGATTG TGTCTGCTTT AGAGTACTTG CACGGCAAGG GCATCATTCA CAGGGACCTT
AAACCGGAAA ACATTTTGTT AAATGAAGAT ATGCACATCC AGATCACAGA TTTTGGAACA
GCAAAAGTCT TATCCCCAGA GAGCAAACAA GCCAGGGCCA ACTCATTCGT GGGAACAGCG
CAGTACGTTT CTCCAGAGCT GCTCACGGAG AAGTCCGCCT GTAAGAGTTC AGACCTTTGG
GCTCTTGGAT GCATAATATA CCAGCTTGTG GCAGGACTCC CACCATTCCG AGCTGGAAAC
GAGTATCTTA TATTTCAGAA GATCATTAAG TTGGAATATG ACTTTCCAGA AAAATTCTTC
CCTAAGGCAA GAGACCTCGT GGAGAAACTT TTGGTTTTAG ATGCCACAAA GCGGTTAGGC
TGTGAGGAAA TGGAAGGATA CGGACCTCTT AAAGCACACC CGTTCTTCGA GTCCGTCACG
TGGGAGAACC TGCACCAGCA GACGCCTCCG AAGCTCACCG CTTACCTGCC GGCTATGTCG
GAAGACGACG AGGACTGCTA TGGCAATTAT GACAATCTCC TGAGCCAGTT TGGCTGCATG
CAGGTGTCTT CGTCCTCCTC CTCACACTCC CTGTCAGCCT CCGACACGGG CCTGCCCCAG
AGGTCAGGCA GCAACATAGA GCAGTACATT CACGATCTGG ACTCGAACTC CTTTGAACTG
GACTTACAGT TTTCCGAAGA TGAGAAGAGG TTGTTGTTGG AGAAGCAGGC TGGCGGAAAC
CCTTGGCACC AGTTTGTAGA AAATAATTTA ATACTAAAGA TGGGCCCAGT GGATAAGCGG
AAGGGTTTAT TTGCAAGACG ACGACAGCTG TTGCTCACAG AAGGACCACA TTTATATTAT
GTGGATCCTG TCAACAAAGT TCTGAAAGGT GAAATTCCTT GGTCACAAGA ACTTCGACCA
GAGGCCAAGA ATTTTAAAAC TTTCTTTGTC CACACGCCTA ACAGGACGTA TTATCTGATG
GACCCCAGCG GGAACGCACA CAAGTGGTGC AGGAAGATCC AGGAGGTTTG GAGGCAGCGA
TACCAGAGCC ACCCGGACGC CGCTGTGCAG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011520823.1
CDS947..2719
Misc Feature(1)1286..2074(+)
Misc Feature(2)1292..2074(+)
Misc Feature(3)1310..1897(+)
Misc Feature(4)1310..1717(+)
Misc Feature(5)1322..1897(+)
Misc Feature(6)1712..1789(+)
Misc Feature(7)2369..2689(+)
Misc Feature(8)2441..2611(+)
Translation

Target ORF information:

RefSeq Version XM_011522521
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011522521

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGTTGAAGA TGTGGGTTAG TGAAATTGAG ACCTGGCGCT TCCTGAGGCT GGCAGCCCGG 
TTGAGCCTGA GGACCAGACT TGAGGCAGTT GGCAGTCCGC GCCTCGTTGC CCTCTGTCTG
CCCTGGTATG ACGCCGTGCC CATCCAGTCC AGCGTGGTGT TATGTTCCTG CCCATCCCCA
TCAATGGTGA GGACCCAGAC TGAGTCCAGC ACGCCCCCTG GCATTCCTGG TGGCAGCAGG
CAGGGCCCCG CCATGGACGG CACTGCAGCC GAGCCTCGGC CCGGCGCCGG CTCCCTGCAG
CATGCCCAGC CTCCGCCGCA GCCTCGGAAG AAGCGGCCTG AGGACTTCAA GTTTGGGAAA
ATCCTTGGGG AAGGCTCTTT TTCCACGGTT GTCCTGGCTC GAGAACTGGC AACCTCCAGA
GAATATGCGA TTAAAATTCT GGAGAAGCGA CATATCATAA AAGAGAACAA GGTCCCCTAT
GTAACCAGAG AGCGGGATGT CATGTCGCGC CTGGATCACC CCTTCTTTGT TAAGCTTTAC
TTCACATTTC AGGACGACGA GAAGCTGTAT TTCGGCCTTA GTTATGCCAA AAATGGAGAA
CTACTTAAAT ATATTCGCAA AATCGGTTCA TTCGATGAGA CCTGTACCCG ATTTTACACG
GCTGAGATTG TGTCTGCTTT AGAGTACTTG CACGGCAAGG GCATCATTCA CAGGGACCTT
AAACCGGAAA ACATTTTGTT AAATGAAGAT ATGCACATCC AGATCACAGA TTTTGGAACA
GCAAAAGTCT TATCCCCAGA GAGCAAACAA GCCAGGGCCA ACTCATTCGT GGGAACAGCG
CAGTACGTTT CTCCAGAGCT GCTCACGGAG AAGTCCGCCT GTAAGAGTTC AGACCTTTGG
GCTCTTGGAT GCATAATATA CCAGCTTGTG GCAGGACTCC CACCATTCCG AGCTGGAAAC
GAGTATCTTA TATTTCAGAA GATCATTAAG TTGGAATATG ACTTTCCAGA AAAATTCTTC
CCTAAGGCAA GAGACCTCGT GGAGAAACTT TTGGTTTTAG ATGCCACAAA GCGGTTAGGC
TGTGAGGAAA TGGAAGGATA CGGACCTCTT AAAGCACACC CGTTCTTCGA GTCCGTCACG
TGGGAGAACC TGCACCAGCA GACGCCTCCG AAGCTCACCG CTTACCTGCC GGCTATGTCG
GAAGACGACG AGGACTGCTA TGGCAATTAT GACAATCTCC TGAGCCAGTT TGGCTGCATG
CAGGTGTCTT CGTCCTCCTC CTCACACTCC CTGTCAGCCT CCGACACGGG CCTGCCCCAG
AGGTCAGGCA GCAACATAGA GCAGTACATT CACGATCTGG ACTCGAACTC CTTTGAACTG
GACTTACAGT TTTCCGAAGA TGAGAAGAGG TTGTTGTTGG AGAAGCAGGC TGGCGGAAAC
CCTTGGCACC AGTTTGTAGA AAATAATTTA ATACTAAAGA TGGGCCCAGT GGATAAGCGG
AAGGGTTTAT TTGCAAGACG ACGACAGCTG TTGCTCACAG AAGGACCACA TTTATATTAT
GTGGATCCTG TCAACAAAGT TCTGAAAGGT GAAATTCCTT GGTCACAAGA ACTTCGACCA
GAGGCCAAGA ATTTTAAAAC TTTCTTTGTC CACACGCCTA ACAGGACGTA TTATCTGATG
GACCCCAGCG GGAACGCACA CAAGTGGTGC AGGAAGATCC AGGAGGTTTG GAGGCAGCGA
TACCAGAGCC ACCCGGACGC CGCTGTGCAG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59607
Clone ID Related Accession (Same CDS sequence) XM_011522522
Accession Version XM_011522522.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1677bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 3-phosphoinositide-dependent protein kinase 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010393.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGGTCGGA GAGCGGTGGC TTACCCACAG TATGACGCCG TGCCCATCCA GTCCAGCGTG 
GTGTTATGTT CCTGCCCATC CCCATCAATG GTGAGGACCC AGACTGAGTC CAGCACGCCC
CCTGGCATTC CTGGTGGCAG CAGGCAGGGC CCCGCCATGG ACGGCACTGC AGCCGAGCCT
CGGCCCGGCG CCGGCTCCCT GCAGCATGCC CAGCCTCCGC CGCAGCCTCG GAAGAAGCGG
CCTGAGGACT TCAAGTTTGG GAAAATCCTT GGGGAAGGCT CTTTTTCCAC GGTTGTCCTG
GCTCGAGAAC TGGCAACCTC CAGAGAATAT GCGATTAAAA TTCTGGAGAA GCGACATATC
ATAAAAGAGA ACAAGGTCCC CTATGTAACC AGAGAGCGGG ATGTCATGTC GCGCCTGGAT
CACCCCTTCT TTGTTAAGCT TTACTTCACA TTTCAGGACG ACGAGAAGCT GTATTTCGGC
CTTAGTTATG CCAAAAATGG AGAACTACTT AAATATATTC GCAAAATCGG TTCATTCGAT
GAGACCTGTA CCCGATTTTA CACGGCTGAG ATTGTGTCTG CTTTAGAGTA CTTGCACGGC
AAGGGCATCA TTCACAGGGA CCTTAAACCG GAAAACATTT TGTTAAATGA AGATATGCAC
ATCCAGATCA CAGATTTTGG AACAGCAAAA GTCTTATCCC CAGAGAGCAA ACAAGCCAGG
GCCAACTCAT TCGTGGGAAC AGCGCAGTAC GTTTCTCCAG AGCTGCTCAC GGAGAAGTCC
GCCTGTAAGA GTTCAGACCT TTGGGCTCTT GGATGCATAA TATACCAGCT TGTGGCAGGA
CTCCCACCAT TCCGAGCTGG AAACGAGTAT CTTATATTTC AGAAGATCAT TAAGTTGGAA
TATGACTTTC CAGAAAAATT CTTCCCTAAG GCAAGAGACC TCGTGGAGAA ACTTTTGGTT
TTAGATGCCA CAAAGCGGTT AGGCTGTGAG GAAATGGAAG GATACGGACC TCTTAAAGCA
CACCCGTTCT TCGAGTCCGT CACGTGGGAG AACCTGCACC AGCAGACGCC TCCGAAGCTC
ACCGCTTACC TGCCGGCTAT GTCGGAAGAC GACGAGGACT GCTATGGCAA TTATGACAAT
CTCCTGAGCC AGTTTGGCTG CATGCAGGTG TCTTCGTCCT CCTCCTCACA CTCCCTGTCA
GCCTCCGACA CGGGCCTGCC CCAGAGGTCA GGCAGCAACA TAGAGCAGTA CATTCACGAT
CTGGACTCGA ACTCCTTTGA ACTGGACTTA CAGTTTTCCG AAGATGAGAA GAGGTTGTTG
TTGGAGAAGC AGGCTGGCGG AAACCCTTGG CACCAGTTTG TAGAAAATAA TTTAATACTA
AAGATGGGCC CAGTGGATAA GCGGAAGGGT TTATTTGCAA GACGACGACA GCTGTTGCTC
ACAGAAGGAC CACATTTATA TTATGTGGAT CCTGTCAACA AAGTTCTGAA AGGTGAAATT
CCTTGGTCAC AAGAACTTCG ACCAGAGGCC AAGAATTTTA AAACTTTCTT TGTCCACACG
CCTAACAGGA CGTATTATCT GATGGACCCC AGCGGGAACG CACACAAGTG GTGCAGGAAG
ATCCAGGAGG TTTGGAGGCA GCGATACCAG AGCCACCCGG ACGCCGCTGT GCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011520824.1
CDS395..2071
Misc Feature(1)638..1426(+)
Misc Feature(2)644..1426(+)
Misc Feature(3)662..1249(+)
Misc Feature(4)662..1069(+)
Misc Feature(5)674..1249(+)
Misc Feature(6)1064..1141(+)
Misc Feature(7)1721..2041(+)
Misc Feature(8)1793..1963(+)
Translation

Target ORF information:

RefSeq Version XM_011522522
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011522522

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGGTCGGA GAGCGGTGGC TTACCCACAG TATGACGCCG TGCCCATCCA GTCCAGCGTG 
GTGTTATGTT CCTGCCCATC CCCATCAATG GTGAGGACCC AGACTGAGTC CAGCACGCCC
CCTGGCATTC CTGGTGGCAG CAGGCAGGGC CCCGCCATGG ACGGCACTGC AGCCGAGCCT
CGGCCCGGCG CCGGCTCCCT GCAGCATGCC CAGCCTCCGC CGCAGCCTCG GAAGAAGCGG
CCTGAGGACT TCAAGTTTGG GAAAATCCTT GGGGAAGGCT CTTTTTCCAC GGTTGTCCTG
GCTCGAGAAC TGGCAACCTC CAGAGAATAT GCGATTAAAA TTCTGGAGAA GCGACATATC
ATAAAAGAGA ACAAGGTCCC CTATGTAACC AGAGAGCGGG ATGTCATGTC GCGCCTGGAT
CACCCCTTCT TTGTTAAGCT TTACTTCACA TTTCAGGACG ACGAGAAGCT GTATTTCGGC
CTTAGTTATG CCAAAAATGG AGAACTACTT AAATATATTC GCAAAATCGG TTCATTCGAT
GAGACCTGTA CCCGATTTTA CACGGCTGAG ATTGTGTCTG CTTTAGAGTA CTTGCACGGC
AAGGGCATCA TTCACAGGGA CCTTAAACCG GAAAACATTT TGTTAAATGA AGATATGCAC
ATCCAGATCA CAGATTTTGG AACAGCAAAA GTCTTATCCC CAGAGAGCAA ACAAGCCAGG
GCCAACTCAT TCGTGGGAAC AGCGCAGTAC GTTTCTCCAG AGCTGCTCAC GGAGAAGTCC
GCCTGTAAGA GTTCAGACCT TTGGGCTCTT GGATGCATAA TATACCAGCT TGTGGCAGGA
CTCCCACCAT TCCGAGCTGG AAACGAGTAT CTTATATTTC AGAAGATCAT TAAGTTGGAA
TATGACTTTC CAGAAAAATT CTTCCCTAAG GCAAGAGACC TCGTGGAGAA ACTTTTGGTT
TTAGATGCCA CAAAGCGGTT AGGCTGTGAG GAAATGGAAG GATACGGACC TCTTAAAGCA
CACCCGTTCT TCGAGTCCGT CACGTGGGAG AACCTGCACC AGCAGACGCC TCCGAAGCTC
ACCGCTTACC TGCCGGCTAT GTCGGAAGAC GACGAGGACT GCTATGGCAA TTATGACAAT
CTCCTGAGCC AGTTTGGCTG CATGCAGGTG TCTTCGTCCT CCTCCTCACA CTCCCTGTCA
GCCTCCGACA CGGGCCTGCC CCAGAGGTCA GGCAGCAACA TAGAGCAGTA CATTCACGAT
CTGGACTCGA ACTCCTTTGA ACTGGACTTA CAGTTTTCCG AAGATGAGAA GAGGTTGTTG
TTGGAGAAGC AGGCTGGCGG AAACCCTTGG CACCAGTTTG TAGAAAATAA TTTAATACTA
AAGATGGGCC CAGTGGATAA GCGGAAGGGT TTATTTGCAA GACGACGACA GCTGTTGCTC
ACAGAAGGAC CACATTTATA TTATGTGGAT CCTGTCAACA AAGTTCTGAA AGGTGAAATT
CCTTGGTCAC AAGAACTTCG ACCAGAGGCC AAGAATTTTA AAACTTTCTT TGTCCACACG
CCTAACAGGA CGTATTATCT GATGGACCCC AGCGGGAACG CACACAAGTG GTGCAGGAAG
ATCCAGGAGG TTTGGAGGCA GCGATACCAG AGCCACCCGG ACGCCGCTGT GCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48855
Clone ID Related Accession (Same CDS sequence) XM_005255356
Accession Version XM_005255356.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1590bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 3-phosphoinositide-dependent protein kinase 1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010393.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGTGAGGA CCCAGACTGA GTCCAGCACG CCCCCTGGCA TTCCTGGTGG CAGCAGGCAG 
GGCCCCGCCA TGGACGGCAC TGCAGCCGAG CCTCGGCCCG GCGCCGGCTC CCTGCAGCAT
GCCCAGCCTC CGCCGCAGCC TCGGAAGAAG CGGCCTGAGG ACTTCAAGTT TGGGAAAATC
CTTGGGGAAG GCTCTTTTTC CACGGTTGTC CTGGCTCGAG AACTGGCAAC CTCCAGAGAA
TATGCGATTA AAATTCTGGA GAAGCGACAT ATCATAAAAG AGAACAAGGT CCCCTATGTA
ACCAGAGAGC GGGATGTCAT GTCGCGCCTG GATCACCCCT TCTTTGTTAA GCTTTACTTC
ACATTTCAGG ACGACGAGAA GCTGTATTTC GGCCTTAGTT ATGCCAAAAA TGGAGAACTA
CTTAAATATA TTCGCAAAAT CGGTTCATTC GATGAGACCT GTACCCGATT TTACACGGCT
GAGATTGTGT CTGCTTTAGA GTACTTGCAC GGCAAGGGCA TCATTCACAG GGACCTTAAA
CCGGAAAACA TTTTGTTAAA TGAAGATATG CACATCCAGA TCACAGATTT TGGAACAGCA
AAAGTCTTAT CCCCAGAGAG CAAACAAGCC AGGGCCAACT CATTCGTGGG AACAGCGCAG
TACGTTTCTC CAGAGCTGCT CACGGAGAAG TCCGCCTGTA AGAGTTCAGA CCTTTGGGCT
CTTGGATGCA TAATATACCA GCTTGTGGCA GGACTCCCAC CATTCCGAGC TGGAAACGAG
TATCTTATAT TTCAGAAGAT CATTAAGTTG GAATATGACT TTCCAGAAAA ATTCTTCCCT
AAGGCAAGAG ACCTCGTGGA GAAACTTTTG GTTTTAGATG CCACAAAGCG GTTAGGCTGT
GAGGAAATGG AAGGATACGG ACCTCTTAAA GCACACCCGT TCTTCGAGTC CGTCACGTGG
GAGAACCTGC ACCAGCAGAC GCCTCCGAAG CTCACCGCTT ACCTGCCGGC TATGTCGGAA
GACGACGAGG ACTGCTATGG CAATTATGAC AATCTCCTGA GCCAGTTTGG CTGCATGCAG
GTGTCTTCGT CCTCCTCCTC ACACTCCCTG TCAGCCTCCG ACACGGGCCT GCCCCAGAGG
TCAGGCAGCA ACATAGAGCA GTACATTCAC GATCTGGACT CGAACTCCTT TGAACTGGAC
TTACAGTTTT CCGAAGATGA GAAGAGGTTG TTGTTGGAGA AGCAGGCTGG CGGAAACCCT
TGGCACCAGT TTGTAGAAAA TAATTTAATA CTAAAGATGG GCCCAGTGGA TAAGCGGAAG
GGTTTATTTG CAAGACGACG ACAGCTGTTG CTCACAGAAG GACCACATTT ATATTATGTG
GATCCTGTCA ACAAAGTTCT GAAAGGTGAA ATTCCTTGGT CACAAGAACT TCGACCAGAG
GCCAAGAATT TTAAAACTTT CTTTGTCCAC ACGCCTAACA GGACGTATTA TCTGATGGAC
CCCAGCGGGA ACGCACACAA GTGGTGCAGG AAGATCCAGG AGGTTTGGAG GCAGCGATAC
CAGAGCCACC CGGACGCCGC TGTGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005255413.1
CDS327..1916
Misc Feature(1)483..1271(+)
Misc Feature(2)489..1271(+)
Misc Feature(3)507..1094(+)
Misc Feature(4)507..914(+)
Misc Feature(5)519..1094(+)
Misc Feature(6)909..986(+)
Misc Feature(7)1566..1886(+)
Misc Feature(8)1638..1808(+)
Translation

Target ORF information:

RefSeq Version XM_005255356
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005255356

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGTGAGGA CCCAGACTGA GTCCAGCACG CCCCCTGGCA TTCCTGGTGG CAGCAGGCAG 
GGCCCCGCCA TGGACGGCAC TGCAGCCGAG CCTCGGCCCG GCGCCGGCTC CCTGCAGCAT
GCCCAGCCTC CGCCGCAGCC TCGGAAGAAG CGGCCTGAGG ACTTCAAGTT TGGGAAAATC
CTTGGGGAAG GCTCTTTTTC CACGGTTGTC CTGGCTCGAG AACTGGCAAC CTCCAGAGAA
TATGCGATTA AAATTCTGGA GAAGCGACAT ATCATAAAAG AGAACAAGGT CCCCTATGTA
ACCAGAGAGC GGGATGTCAT GTCGCGCCTG GATCACCCCT TCTTTGTTAA GCTTTACTTC
ACATTTCAGG ACGACGAGAA GCTGTATTTC GGCCTTAGTT ATGCCAAAAA TGGAGAACTA
CTTAAATATA TTCGCAAAAT CGGTTCATTC GATGAGACCT GTACCCGATT TTACACGGCT
GAGATTGTGT CTGCTTTAGA GTACTTGCAC GGCAAGGGCA TCATTCACAG GGACCTTAAA
CCGGAAAACA TTTTGTTAAA TGAAGATATG CACATCCAGA TCACAGATTT TGGAACAGCA
AAAGTCTTAT CCCCAGAGAG CAAACAAGCC AGGGCCAACT CATTCGTGGG AACAGCGCAG
TACGTTTCTC CAGAGCTGCT CACGGAGAAG TCCGCCTGTA AGAGTTCAGA CCTTTGGGCT
CTTGGATGCA TAATATACCA GCTTGTGGCA GGACTCCCAC CATTCCGAGC TGGAAACGAG
TATCTTATAT TTCAGAAGAT CATTAAGTTG GAATATGACT TTCCAGAAAA ATTCTTCCCT
AAGGCAAGAG ACCTCGTGGA GAAACTTTTG GTTTTAGATG CCACAAAGCG GTTAGGCTGT
GAGGAAATGG AAGGATACGG ACCTCTTAAA GCACACCCGT TCTTCGAGTC CGTCACGTGG
GAGAACCTGC ACCAGCAGAC GCCTCCGAAG CTCACCGCTT ACCTGCCGGC TATGTCGGAA
GACGACGAGG ACTGCTATGG CAATTATGAC AATCTCCTGA GCCAGTTTGG CTGCATGCAG
GTGTCTTCGT CCTCCTCCTC ACACTCCCTG TCAGCCTCCG ACACGGGCCT GCCCCAGAGG
TCAGGCAGCA ACATAGAGCA GTACATTCAC GATCTGGACT CGAACTCCTT TGAACTGGAC
TTACAGTTTT CCGAAGATGA GAAGAGGTTG TTGTTGGAGA AGCAGGCTGG CGGAAACCCT
TGGCACCAGT TTGTAGAAAA TAATTTAATA CTAAAGATGG GCCCAGTGGA TAAGCGGAAG
GGTTTATTTG CAAGACGACG ACAGCTGTTG CTCACAGAAG GACCACATTT ATATTATGTG
GATCCTGTCA ACAAAGTTCT GAAAGGTGAA ATTCCTTGGT CACAAGAACT TCGACCAGAG
GCCAAGAATT TTAAAACTTT CTTTGTCCAC ACGCCTAACA GGACGTATTA TCTGATGGAC
CCCAGCGGGA ACGCACACAA GTGGTGCAGG AAGATCCAGG AGGTTTGGAG GCAGCGATAC
CAGAGCCACC CGGACGCCGC TGTGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59608
Clone ID Related Accession (Same CDS sequence) XM_011522523
Accession Version XM_011522523.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1392bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 3-phosphoinositide-dependent protein kinase 1 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010393.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGTTGAAGA TGTGGGTTAG TGAAATTGAG ACCTGGCGCT TCCTGAGGCT GGCAGCCCGG 
TTGAGCCTGA GGACCAGACT TGAGGCAGTT GGCAGTCCGC GCCTCGTTGC CCTCTGTCTG
CCCTGGTATG ACGCCGTGCC CATCCAGTCC AGCGTGGTGT TATGTTCCTG CCCATCCCCA
TCAATGGTGA GGACCCAGAC TGAGTCCAGC ACGCCCCCTG GCATTCCTGG TGGCAGCAGG
CAGGGCCCCG CCATGGACGG CACTGCAGCC GAGCCTCGGC CCGGCGCCGG CTCCCTGCAG
CATGCCCAGC CTCCGCCGCA GCCTCGGAAG AAGCGGCCTG AGGACTTCAA GTTTGGGAAA
ATCCTTGGGG AAGGCTCTTT TTCCACGGTT GTCCTGGCTC GAGAACTGGC AACCTCCAGA
GAATATGCGA CCAGGGCCAA CTCATTCGTG GGAACAGCGC AGTACGTTTC TCCAGAGCTG
CTCACGGAGA AGTCCGCCTG TAAGAGTTCA GACCTTTGGG CTCTTGGATG CATAATATAC
CAGCTTGTGG CAGGACTCCC ACCATTCCGA GCTGGAAACG AGTATCTTAT ATTTCAGAAG
ATCATTAAGT TGGAATATGA CTTTCCAGAA AAATTCTTCC CTAAGGCAAG AGACCTCGTG
GAGAAACTTT TGGTTTTAGA TGCCACAAAG CGGTTAGGCT GTGAGGAAAT GGAAGGATAC
GGACCTCTTA AAGCACACCC GTTCTTCGAG TCCGTCACGT GGGAGAACCT GCACCAGCAG
ACGCCTCCGA AGCTCACCGC TTACCTGCCG GCTATGTCGG AAGACGACGA GGACTGCTAT
GGCAATTATG ACAATCTCCT GAGCCAGTTT GGCTGCATGC AGGTGTCTTC GTCCTCCTCC
TCACACTCCC TGTCAGCCTC CGACACGGGC CTGCCCCAGA GGTCAGGCAG CAACATAGAG
CAGTACATTC ACGATCTGGA CTCGAACTCC TTTGAACTGG ACTTACAGTT TTCCGAAGAT
GAGAAGAGGT TGTTGTTGGA GAAGCAGGCT GGCGGAAACC CTTGGCACCA GTTTGTAGAA
AATAATTTAA TACTAAAGAT GGGCCCAGTG GATAAGCGGA AGGGTTTATT TGCAAGACGA
CGACAGCTGT TGCTCACAGA AGGACCACAT TTATATTATG TGGATCCTGT CAACAAAGTT
CTGAAAGGTG AAATTCCTTG GTCACAAGAA CTTCGACCAG AGGCCAAGAA TTTTAAAACT
TTCTTTGTCC ACACGCCTAA CAGGACGTAT TATCTGATGG ACCCCAGCGG GAACGCACAC
AAGTGGTGCA GGAAGATCCA GGAGGTTTGG AGGCAGCGAT ACCAGAGCCA CCCGGACGCC
GCTGTGCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011520825.1
CDS947..2338
Misc Feature(1)1289..1693(+)
Misc Feature(2)1988..2308(+)
Misc Feature(3)2060..2230(+)
Translation

Target ORF information:

RefSeq Version XM_011522523
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011522523

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGTTGAAGA TGTGGGTTAG TGAAATTGAG ACCTGGCGCT TCCTGAGGCT GGCAGCCCGG 
TTGAGCCTGA GGACCAGACT TGAGGCAGTT GGCAGTCCGC GCCTCGTTGC CCTCTGTCTG
CCCTGGTATG ACGCCGTGCC CATCCAGTCC AGCGTGGTGT TATGTTCCTG CCCATCCCCA
TCAATGGTGA GGACCCAGAC TGAGTCCAGC ACGCCCCCTG GCATTCCTGG TGGCAGCAGG
CAGGGCCCCG CCATGGACGG CACTGCAGCC GAGCCTCGGC CCGGCGCCGG CTCCCTGCAG
CATGCCCAGC CTCCGCCGCA GCCTCGGAAG AAGCGGCCTG AGGACTTCAA GTTTGGGAAA
ATCCTTGGGG AAGGCTCTTT TTCCACGGTT GTCCTGGCTC GAGAACTGGC AACCTCCAGA
GAATATGCGA CCAGGGCCAA CTCATTCGTG GGAACAGCGC AGTACGTTTC TCCAGAGCTG
CTCACGGAGA AGTCCGCCTG TAAGAGTTCA GACCTTTGGG CTCTTGGATG CATAATATAC
CAGCTTGTGG CAGGACTCCC ACCATTCCGA GCTGGAAACG AGTATCTTAT ATTTCAGAAG
ATCATTAAGT TGGAATATGA CTTTCCAGAA AAATTCTTCC CTAAGGCAAG AGACCTCGTG
GAGAAACTTT TGGTTTTAGA TGCCACAAAG CGGTTAGGCT GTGAGGAAAT GGAAGGATAC
GGACCTCTTA AAGCACACCC GTTCTTCGAG TCCGTCACGT GGGAGAACCT GCACCAGCAG
ACGCCTCCGA AGCTCACCGC TTACCTGCCG GCTATGTCGG AAGACGACGA GGACTGCTAT
GGCAATTATG ACAATCTCCT GAGCCAGTTT GGCTGCATGC AGGTGTCTTC GTCCTCCTCC
TCACACTCCC TGTCAGCCTC CGACACGGGC CTGCCCCAGA GGTCAGGCAG CAACATAGAG
CAGTACATTC ACGATCTGGA CTCGAACTCC TTTGAACTGG ACTTACAGTT TTCCGAAGAT
GAGAAGAGGT TGTTGTTGGA GAAGCAGGCT GGCGGAAACC CTTGGCACCA GTTTGTAGAA
AATAATTTAA TACTAAAGAT GGGCCCAGTG GATAAGCGGA AGGGTTTATT TGCAAGACGA
CGACAGCTGT TGCTCACAGA AGGACCACAT TTATATTATG TGGATCCTGT CAACAAAGTT
CTGAAAGGTG AAATTCCTTG GTCACAAGAA CTTCGACCAG AGGCCAAGAA TTTTAAAACT
TTCTTTGTCC ACACGCCTAA CAGGACGTAT TATCTGATGG ACCCCAGCGG GAACGCACAC
AAGTGGTGCA GGAAGATCCA GGAGGTTTGG AGGCAGCGAT ACCAGAGCCA CCCGGACGCC
GCTGTGCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59609
Clone ID Related Accession (Same CDS sequence) XM_011522524
Accession Version XM_011522524.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 912bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 3-phosphoinositide-dependent protein kinase 1 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010393.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTTGAAGA TGTGGGTTAG TGAAATTGAG ACCTGGCGCT TCCTGAGGCT GGCAGCCCGG 
TTGAGCCTGA GGACCAGACT TGAGGCAGTT GGCAGTCCGC GCCTCGTTGC CCTCTGTCTG
CCCTGGTATG ACGCCGTGCC CATCCAGTCC AGCGTGGTGT TATGTTCCTG CCCATCCCCA
TCAATGGTGA GGACCCAGAC TGAGTCCAGC ACGCCCCCTG GCATTCCTGG TGGCAGCAGG
CAGGGCCCCG CCATGGACGG CACTGCAGCC GAGCCTCGGC CCGGCGCCGG CTCCCTGCAG
CATGCCCAGC CTCCGCCGCA GCCTCGGAAG AAGCGGCCTG AGGACTTCAA GTTTGGGAAA
ATCCTTGGGG AAGGCTCTTT TTCCACGGTT GTCCTGGCTC GAGAACTGGC AACCTCCAGA
GAATATGCGA TTAAAATTCT GGAGAAGCGA CATATCATAA AAGAGAACAA GGTCCCCTAT
GTAACCAGAG AGCGGGATGT CATGTCGCGC CTGGATCACC CCTTCTTTGT TAAGCTTTAC
TTCACATTTC AGGACGACGA GAAGCTGTAT TTCGGCCTTA GTTATGCCAA AAATGGAGAA
CTACTTAAAT ATATTCGCAA AATCGGTTCA TTCGATGAGA CCTGTACCCG ATTTTACACG
GCTGAGATTG TGTCTGCTTT AGAGTACTTG CACGGCAAGG GCATCATTCA CAGGGACCTT
AAACCGGAAA ACATTTTGTT AAATGAAGAT ATGCACATCC AGATCACAGA TTTTGGAACA
GCAAAAGTCT TATCCCCAGA GAGCAAACAA GACCCTCAGA CTAGCTGCCG TTGCGCTTCC
CACTGTGCTG TGGACTCCAG ACGCTCTCCT GAGGGCCACA GCTGCCTTGA GCTTGCCGCC
ATTCTAGCTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011520826.1
CDS947..1858
Misc Feature(1)1286..>1819(+)
Misc Feature(2)1292..>1753(+)
Misc Feature(3)1310..1717(+)
Translation

Target ORF information:

RefSeq Version XM_011522524
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011522524

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTTGAAGA TGTGGGTTAG TGAAATTGAG ACCTGGCGCT TCCTGAGGCT GGCAGCCCGG 
TTGAGCCTGA GGACCAGACT TGAGGCAGTT GGCAGTCCGC GCCTCGTTGC CCTCTGTCTG
CCCTGGTATG ACGCCGTGCC CATCCAGTCC AGCGTGGTGT TATGTTCCTG CCCATCCCCA
TCAATGGTGA GGACCCAGAC TGAGTCCAGC ACGCCCCCTG GCATTCCTGG TGGCAGCAGG
CAGGGCCCCG CCATGGACGG CACTGCAGCC GAGCCTCGGC CCGGCGCCGG CTCCCTGCAG
CATGCCCAGC CTCCGCCGCA GCCTCGGAAG AAGCGGCCTG AGGACTTCAA GTTTGGGAAA
ATCCTTGGGG AAGGCTCTTT TTCCACGGTT GTCCTGGCTC GAGAACTGGC AACCTCCAGA
GAATATGCGA TTAAAATTCT GGAGAAGCGA CATATCATAA AAGAGAACAA GGTCCCCTAT
GTAACCAGAG AGCGGGATGT CATGTCGCGC CTGGATCACC CCTTCTTTGT TAAGCTTTAC
TTCACATTTC AGGACGACGA GAAGCTGTAT TTCGGCCTTA GTTATGCCAA AAATGGAGAA
CTACTTAAAT ATATTCGCAA AATCGGTTCA TTCGATGAGA CCTGTACCCG ATTTTACACG
GCTGAGATTG TGTCTGCTTT AGAGTACTTG CACGGCAAGG GCATCATTCA CAGGGACCTT
AAACCGGAAA ACATTTTGTT AAATGAAGAT ATGCACATCC AGATCACAGA TTTTGGAACA
GCAAAAGTCT TATCCCCAGA GAGCAAACAA GACCCTCAGA CTAGCTGCCG TTGCGCTTCC
CACTGTGCTG TGGACTCCAG ACGCTCTCCT GAGGGCCACA GCTGCCTTGA GCTTGCCGCC
ATTCTAGCTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu13008
Clone ID Related Accession (Same CDS sequence) NM_002613
Accession Version NM_002613.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1671bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 3-phosphoinositide-dependent protein kinase 1 isoform 1
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC093525.3, BC012103.1 and AC141586.2. On May 17, 2012 this sequence version replaced gi:60498971. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012103.1, AK222581.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCCAGGA CCACCAGCCA GCTGTATGAC GCCGTGCCCA TCCAGTCCAG CGTGGTGTTA 
TGTTCCTGCC CATCCCCATC AATGGTGAGG ACCCAGACTG AGTCCAGCAC GCCCCCTGGC
ATTCCTGGTG GCAGCAGGCA GGGCCCCGCC ATGGACGGCA CTGCAGCCGA GCCTCGGCCC
GGCGCCGGCT CCCTGCAGCA TGCCCAGCCT CCGCCGCAGC CTCGGAAGAA GCGGCCTGAG
GACTTCAAGT TTGGGAAAAT CCTTGGGGAA GGCTCTTTTT CCACGGTTGT CCTGGCTCGA
GAACTGGCAA CCTCCAGAGA ATATGCGATT AAAATTCTGG AGAAGCGACA TATCATAAAA
GAGAACAAGG TCCCCTATGT AACCAGAGAG CGGGATGTCA TGTCGCGCCT GGATCACCCC
TTCTTTGTTA AGCTTTACTT CACATTTCAG GACGACGAGA AGCTGTATTT CGGCCTTAGT
TATGCCAAAA ATGGAGAACT ACTTAAATAT ATTCGCAAAA TCGGTTCATT CGATGAGACC
TGTACCCGAT TTTACACGGC TGAGATTGTG TCTGCTTTAG AGTACTTGCA CGGCAAGGGC
ATCATTCACA GGGACCTTAA ACCGGAAAAC ATTTTGTTAA ATGAAGATAT GCACATCCAG
ATCACAGATT TTGGAACAGC AAAAGTCTTA TCCCCAGAGA GCAAACAAGC CAGGGCCAAC
TCATTCGTGG GAACAGCGCA GTACGTTTCT CCAGAGCTGC TCACGGAGAA GTCCGCCTGT
AAGAGTTCAG ACCTTTGGGC TCTTGGATGC ATAATATACC AGCTTGTGGC AGGACTCCCA
CCATTCCGAG CTGGAAACGA GTATCTTATA TTTCAGAAGA TCATTAAGTT GGAATATGAC
TTTCCAGAAA AATTCTTCCC TAAGGCAAGA GACCTCGTGG AGAAACTTTT GGTTTTAGAT
GCCACAAAGC GGTTAGGCTG TGAGGAAATG GAAGGATACG GACCTCTTAA AGCACACCCG
TTCTTCGAGT CCGTCACGTG GGAGAACCTG CACCAGCAGA CGCCTCCGAA GCTCACCGCT
TACCTGCCGG CTATGTCGGA AGACGACGAG GACTGCTATG GCAATTATGA CAATCTCCTG
AGCCAGTTTG GCTGCATGCA GGTGTCTTCG TCCTCCTCCT CACACTCCCT GTCAGCCTCC
GACACGGGCC TGCCCCAGAG GTCAGGCAGC AACATAGAGC AGTACATTCA CGATCTGGAC
TCGAACTCCT TTGAACTGGA CTTACAGTTT TCCGAAGATG AGAAGAGGTT GTTGTTGGAG
AAGCAGGCTG GCGGAAACCC TTGGCACCAG TTTGTAGAAA ATAATTTAAT ACTAAAGATG
GGCCCAGTGG ATAAGCGGAA GGGTTTATTT GCAAGACGAC GACAGCTGTT GCTCACAGAA
GGACCACATT TATATTATGT GGATCCTGTC AACAAAGTTC TGAAAGGTGA AATTCCTTGG
TCACAAGAAC TTCGACCAGA GGCCAAGAAT TTTAAAACTT TCTTTGTCCA CACGCCTAAC
AGGACGTATT ATCTGATGGA CCCCAGCGGG AACGCACACA AGTGGTGCAG GAAGATCCAG
GAGGTTTGGA GGCAGCGATA CCAGAGCCAC CCGGACGCCG CTGTGCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002604.1
CDS150..1820
Misc Feature(1)174..176(+)
Misc Feature(2)174..176(+)
Misc Feature(3)222..224(+)
Misc Feature(4)222..224(+)
Misc Feature(5)246..248(+)
Misc Feature(6)387..1175(+)
Misc Feature(7)393..1175(+)
Misc Feature(8)411..998(+)
Misc Feature(9)411..818(+)
Misc Feature(10)423..998(+)
Misc Feature(11)813..890(+)
Misc Feature(12)870..872(+)
Misc Feature(13)870..872(+)
Misc Feature(14)870..872(+)
Misc Feature(15)870..872(+)
Misc Feature(16)882..884(+)
Misc Feature(17)1059..1061(+)
Misc Feature(18)1209..1211(+)
Misc Feature(19)1266..1268(+)
Misc Feature(20)1266..1268(+)
Misc Feature(21)1275..1277(+)
Misc Feature(22)1326..1328(+)
Misc Feature(23)1326..1328(+)
Misc Feature(24)1329..1331(+)
Misc Feature(25)1335..1337(+)
Misc Feature(26)1335..1337(+)
Misc Feature(27)1341..1343(+)
Misc Feature(28)1377..1379(+)
Misc Feature(29)1377..1379(+)
Misc Feature(30)1470..1790(+)
Misc Feature(31)1542..1712(+)
Misc Feature(32)1602..1604(+)
Misc Feature(33)1650..1652(+)
Misc Feature(34)1686..1688(+)
Misc Feature(35)1686..1688(+)
Misc Feature(36)1734..1736(+)
Exon (1)1..173
Gene:PDPK1
Gene Synonym:
Exon (2)174..434
Gene:PDPK1
Gene Synonym:
Exon (3)435..477
Gene:PDPK1
Gene Synonym:
Exon (4)478..615
Gene:PDPK1
Gene Synonym:
Exon (5)616..760
Gene:PDPK1
Gene Synonym:
Exon (6)761..858
Gene:PDPK1
Gene Synonym:
Exon (7)859..934
Gene:PDPK1
Gene Synonym:
Exon (8)935..1003
Gene:PDPK1
Gene Synonym:
Exon (9)1004..1100
Gene:PDPK1
Gene Synonym:
Exon (10)1101..1274
Gene:PDPK1
Gene Synonym:
Exon (11)1275..1492
Gene:PDPK1
Gene Synonym:
Exon (12)1493..1550
Gene:PDPK1
Gene Synonym:
Exon (13)1551..1703
Gene:PDPK1
Gene Synonym:
Exon (14)1704..7243
Gene:PDPK1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002613
Organism Homo sapiens (human)
Definition Homo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002613

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCCAGGA CCACCAGCCA GCTGTATGAC GCCGTGCCCA TCCAGTCCAG CGTGGTGTTA 
TGTTCCTGCC CATCCCCATC AATGGTGAGG ACCCAGACTG AGTCCAGCAC GCCCCCTGGC
ATTCCTGGTG GCAGCAGGCA GGGCCCCGCC ATGGACGGCA CTGCAGCCGA GCCTCGGCCC
GGCGCCGGCT CCCTGCAGCA TGCCCAGCCT CCGCCGCAGC CTCGGAAGAA GCGGCCTGAG
GACTTCAAGT TTGGGAAAAT CCTTGGGGAA GGCTCTTTTT CCACGGTTGT CCTGGCTCGA
GAACTGGCAA CCTCCAGAGA ATATGCGATT AAAATTCTGG AGAAGCGACA TATCATAAAA
GAGAACAAGG TCCCCTATGT AACCAGAGAG CGGGATGTCA TGTCGCGCCT GGATCACCCC
TTCTTTGTTA AGCTTTACTT CACATTTCAG GACGACGAGA AGCTGTATTT CGGCCTTAGT
TATGCCAAAA ATGGAGAACT ACTTAAATAT ATTCGCAAAA TCGGTTCATT CGATGAGACC
TGTACCCGAT TTTACACGGC TGAGATTGTG TCTGCTTTAG AGTACTTGCA CGGCAAGGGC
ATCATTCACA GGGACCTTAA ACCGGAAAAC ATTTTGTTAA ATGAAGATAT GCACATCCAG
ATCACAGATT TTGGAACAGC AAAAGTCTTA TCCCCAGAGA GCAAACAAGC CAGGGCCAAC
TCATTCGTGG GAACAGCGCA GTACGTTTCT CCAGAGCTGC TCACGGAGAA GTCCGCCTGT
AAGAGTTCAG ACCTTTGGGC TCTTGGATGC ATAATATACC AGCTTGTGGC AGGACTCCCA
CCATTCCGAG CTGGAAACGA GTATCTTATA TTTCAGAAGA TCATTAAGTT GGAATATGAC
TTTCCAGAAA AATTCTTCCC TAAGGCAAGA GACCTCGTGG AGAAACTTTT GGTTTTAGAT
GCCACAAAGC GGTTAGGCTG TGAGGAAATG GAAGGATACG GACCTCTTAA AGCACACCCG
TTCTTCGAGT CCGTCACGTG GGAGAACCTG CACCAGCAGA CGCCTCCGAA GCTCACCGCT
TACCTGCCGG CTATGTCGGA AGACGACGAG GACTGCTATG GCAATTATGA CAATCTCCTG
AGCCAGTTTG GCTGCATGCA GGTGTCTTCG TCCTCCTCCT CACACTCCCT GTCAGCCTCC
GACACGGGCC TGCCCCAGAG GTCAGGCAGC AACATAGAGC AGTACATTCA CGATCTGGAC
TCGAACTCCT TTGAACTGGA CTTACAGTTT TCCGAAGATG AGAAGAGGTT GTTGTTGGAG
AAGCAGGCTG GCGGAAACCC TTGGCACCAG TTTGTAGAAA ATAATTTAAT ACTAAAGATG
GGCCCAGTGG ATAAGCGGAA GGGTTTATTT GCAAGACGAC GACAGCTGTT GCTCACAGAA
GGACCACATT TATATTATGT GGATCCTGTC AACAAAGTTC TGAAAGGTGA AATTCCTTGG
TCACAAGAAC TTCGACCAGA GGCCAAGAAT TTTAAAACTT TCTTTGTCCA CACGCCTAAC
AGGACGTATT ATCTGATGGA CCCCAGCGGG AACGCACACA AGTGGTGCAG GAAGATCCAG
GAGGTTTGGA GGCAGCGATA CCAGAGCCAC CCGGACGCCG CTGTGCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu13009
Clone ID Related Accession (Same CDS sequence) NM_031268
Accession Version NM_031268.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1290bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 3-phosphoinositide-dependent protein kinase 1 isoform 2
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC093525.3, BC033494.1 and AC141586.2. On May 17, 2012 this sequence version replaced gi:60498970. Transcript Variant: This variant (2) lacks three consecutive exons in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033494.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCCAGGA CCACCAGCCA GCTGTATGAC GCCGTGCCCA TCCAGTCCAG CGTGGTGTTA 
TGTTCCTGCC CATCCCCATC AATGGTGAGG ACCCAGACTG AGTCCAGCAC GCCCCCTGGC
ATTCCTGGTG GCAGCAGGCA GGGCCCCGCC ATGGACGGCA CTGCAGCCGA GCCTCGGCCC
GGCGCCGGCT CCCTGCAGCA TGCCCAGCCT CCGCCGCAGC CTCGGAAGAA GCGGCCTGAG
GACTTCAAGT TTGGGAAAAT CCTTGGGGAA GGCTCTTTTT CCACGGTTGT CCTGGCTCGA
GAACTGGCAA CCTCCAGAGA ATATGCGACC AGGGCCAACT CATTCGTGGG AACAGCGCAG
TACGTTTCTC CAGAGCTGCT CACGGAGAAG TCCGCCTGTA AGAGTTCAGA CCTTTGGGCT
CTTGGATGCA TAATATACCA GCTTGTGGCA GGACTCCCAC CATTCCGAGC TGGAAACGAG
TATCTTATAT TTCAGAAGAT CATTAAGTTG GAATATGACT TTCCAGAAAA ATTCTTCCCT
AAGGCAAGAG ACCTCGTGGA GAAACTTTTG GTTTTAGATG CCACAAAGCG GTTAGGCTGT
GAGGAAATGG AAGGATACGG ACCTCTTAAA GCACACCCGT TCTTCGAGTC CGTCACGTGG
GAGAACCTGC ACCAGCAGAC GCCTCCGAAG CTCACCGCTT ACCTGCCGGC TATGTCGGAA
GACGACGAGG ACTGCTATGG CAATTATGAC AATCTCCTGA GCCAGTTTGG CTGCATGCAG
GTGTCTTCGT CCTCCTCCTC ACACTCCCTG TCAGCCTCCG ACACGGGCCT GCCCCAGAGG
TCAGGCAGCA ACATAGAGCA GTACATTCAC GATCTGGACT CGAACTCCTT TGAACTGGAC
TTACAGTTTT CCGAAGATGA GAAGAGGTTG TTGTTGGAGA AGCAGGCTGG CGGAAACCCT
TGGCACCAGT TTGTAGAAAA TAATTTAATA CTAAAGATGG GCCCAGTGGA TAAGCGGAAG
GGTTTATTTG CAAGACGACG ACAGCTGTTG CTCACAGAAG GACCACATTT ATATTATGTG
GATCCTGTCA ACAAAGTTCT GAAAGGTGAA ATTCCTTGGT CACAAGAACT TCGACCAGAG
GCCAAGAATT TTAAAACTTT CTTTGTCCAC ACGCCTAACA GGACGTATTA TCTGATGGAC
CCCAGCGGGA ACGCACACAA GTGGTGCAGG AAGATCCAGG AGGTTTGGAG GCAGCGATAC
CAGAGCCACC CGGACGCCGC TGTGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_112558.2
CDS150..1439
Misc Feature(1)174..176(+)
Misc Feature(2)174..176(+)
Misc Feature(3)222..224(+)
Misc Feature(4)222..224(+)
Misc Feature(5)246..248(+)
Misc Feature(6)390..794(+)
Misc Feature(7)489..491(+)
Misc Feature(8)489..491(+)
Misc Feature(9)489..491(+)
Misc Feature(10)501..503(+)
Misc Feature(11)885..887(+)
Misc Feature(12)945..947(+)
Misc Feature(13)954..956(+)
Misc Feature(14)996..998(+)
Misc Feature(15)1089..1409(+)
Misc Feature(16)1161..1331(+)
Misc Feature(17)1221..1223(+)
Misc Feature(18)1305..1307(+)
Exon (1)1..173
Gene:PDPK1
Gene Synonym:
Exon (2)174..434
Gene:PDPK1
Gene Synonym:
Exon (3)435..477
Gene:PDPK1
Gene Synonym:
Exon (4)478..553
Gene:PDPK1
Gene Synonym:
Exon (5)554..622
Gene:PDPK1
Gene Synonym:
Exon (6)623..719
Gene:PDPK1
Gene Synonym:
Exon (7)720..893
Gene:PDPK1
Gene Synonym:
Exon (8)894..1111
Gene:PDPK1
Gene Synonym:
Exon (9)1112..1169
Gene:PDPK1
Gene Synonym:
Exon (10)1170..1322
Gene:PDPK1
Gene Synonym:
Exon (11)1323..6862
Gene:PDPK1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_031268
Organism Homo sapiens (human)
Definition Homo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_031268

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCCAGGA CCACCAGCCA GCTGTATGAC GCCGTGCCCA TCCAGTCCAG CGTGGTGTTA 
TGTTCCTGCC CATCCCCATC AATGGTGAGG ACCCAGACTG AGTCCAGCAC GCCCCCTGGC
ATTCCTGGTG GCAGCAGGCA GGGCCCCGCC ATGGACGGCA CTGCAGCCGA GCCTCGGCCC
GGCGCCGGCT CCCTGCAGCA TGCCCAGCCT CCGCCGCAGC CTCGGAAGAA GCGGCCTGAG
GACTTCAAGT TTGGGAAAAT CCTTGGGGAA GGCTCTTTTT CCACGGTTGT CCTGGCTCGA
GAACTGGCAA CCTCCAGAGA ATATGCGACC AGGGCCAACT CATTCGTGGG AACAGCGCAG
TACGTTTCTC CAGAGCTGCT CACGGAGAAG TCCGCCTGTA AGAGTTCAGA CCTTTGGGCT
CTTGGATGCA TAATATACCA GCTTGTGGCA GGACTCCCAC CATTCCGAGC TGGAAACGAG
TATCTTATAT TTCAGAAGAT CATTAAGTTG GAATATGACT TTCCAGAAAA ATTCTTCCCT
AAGGCAAGAG ACCTCGTGGA GAAACTTTTG GTTTTAGATG CCACAAAGCG GTTAGGCTGT
GAGGAAATGG AAGGATACGG ACCTCTTAAA GCACACCCGT TCTTCGAGTC CGTCACGTGG
GAGAACCTGC ACCAGCAGAC GCCTCCGAAG CTCACCGCTT ACCTGCCGGC TATGTCGGAA
GACGACGAGG ACTGCTATGG CAATTATGAC AATCTCCTGA GCCAGTTTGG CTGCATGCAG
GTGTCTTCGT CCTCCTCCTC ACACTCCCTG TCAGCCTCCG ACACGGGCCT GCCCCAGAGG
TCAGGCAGCA ACATAGAGCA GTACATTCAC GATCTGGACT CGAACTCCTT TGAACTGGAC
TTACAGTTTT CCGAAGATGA GAAGAGGTTG TTGTTGGAGA AGCAGGCTGG CGGAAACCCT
TGGCACCAGT TTGTAGAAAA TAATTTAATA CTAAAGATGG GCCCAGTGGA TAAGCGGAAG
GGTTTATTTG CAAGACGACG ACAGCTGTTG CTCACAGAAG GACCACATTT ATATTATGTG
GATCCTGTCA ACAAAGTTCT GAAAGGTGAA ATTCCTTGGT CACAAGAACT TCGACCAGAG
GCCAAGAATT TTAAAACTTT CTTTGTCCAC ACGCCTAACA GGACGTATTA TCTGATGGAC
CCCAGCGGGA ACGCACACAA GTGGTGCAGG AAGATCCAGG AGGTTTGGAG GCAGCGATAC
CAGAGCCACC CGGACGCCGC TGTGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu13007
Clone ID Related Accession (Same CDS sequence) NM_001261816
Accession Version NM_001261816.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1365bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 3-phosphoinositide-dependent protein kinase 1 isoform 3
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC093525.3, AB209835.1 and AC141586.2. Transcript Variant: This variant (3) lacks two consecutive exons in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (3) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209835.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004, SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCCAGGA CCACCAGCCA GCTGTATGAC GCCGTGCCCA TCCAGTCCAG CGTGGTGTTA 
TGTTCCTGCC CATCCCCATC AATGGTGAGG ACCCAGACTG AGTCCAGCAC GCCCCCTGGC
ATTCCTGGTG GCAGCAGGCA GGGCCCCGCC ATGGACGGCA CTGCAGCCGA GCCTCGGCCC
GGCGCCGGCT CCCTGCAGCA TGCCCAGCCT CCGCCGCAGC CTCGGAAGAA GCGGCCTGAG
GACTTCAAGT TTGGGAAAAT CCTTGGGGAA GGCTCTTTTT CCACGGTTGT CCTGGCTCGA
GAACTGGCAA CCTCCAGAGA ATATGCGATT AAAATTCTGG AGAAGCGACA TATCATAAAA
GAGAACAAGG TCCCCTATGT AACCAGAGAG CGGGATGTCA TGTCGCGCCT GGATCACCCC
TTCTTTGTTA AGCTTTACTT CACATTTCAG GACGACGAGA AGCTGTATTT CGGCCTTAGT
TATGCCAAAA ATGGAGAACT ACTTAAATAT ATTCGCAAAA TCGGTTCATT CGATGAGACC
TGTACCCGAT TTTACACGGC TGAGATTGTG TCTGCTTTAG AGTACTTGCA CGGCAAGGGC
ATCATTCACA GGGACCTTAA ACCGGAAAAC ATTTTGTTAA ATGAAGATAT GCACATCCAG
ATCACAGATT TTGGAACAGC AAAAGTCTTA TCCCCAGAGA GCAAACAAGC CAGGGCCAAC
TCATTCGTGG GAACAGCGCA GTACGTTTCT CCAGAGCTGC TCACGGAGAA GTCCGCCTGT
AAGAGTTCAG ACCTTTGGGC TCTTGGATGC ATAATATACC AGCTTGTGGC AGGACTCCCA
CCATTCCGAG CTGGAAACGA GTATCTTATA TTTCAGAAGA TCATTAAGTT GGAATATGAC
TTTCCAGAAA AATTCTTCCC TAAGGCAAGA GACCTCGTGG AGAAACTTTT GGTTTTAGAT
GCCACAAAGC GGTTAGGCTG TGAGGAAATG GAAGGATACG GACCTCTTAA AGCACACCCG
TTCTTCGAGT CCGTCACGTG GGAGAACCTG CACCAGCAGA CGCCTCCGAA GCTCACCGCT
TACCTGCCGG CTATGTCGGA AGACGACGAG GACTGCTATG GCAATTATGA CAATCTCCTG
AGCCAGTTTG GCTGCATGCA GGTGTCTTCG TCCTCCTCCT CACACTCCCT GTCAGCCTCC
GACACGGGCC TGCCCCAGAG GTCAGGCAGC AACATAGAGC AGTACATTCA CGATCTGGAC
TCGAACTCCT TTGAACTGGA CTTACAGTTT TCCGAAGATG AGAAGAGGTT GTTGTTGGAG
AAGCAGGCTG GCGGAAACCC TTGCCTAACA GGACGTATTA TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001248745.1
CDS150..1514
Misc Feature(1)174..176(+)
Misc Feature(2)222..224(+)
Misc Feature(3)387..1175(+)
Misc Feature(4)393..1175(+)
Misc Feature(5)411..998(+)
Misc Feature(6)411..818(+)
Misc Feature(7)423..998(+)
Misc Feature(8)813..890(+)
Misc Feature(9)870..872(+)
Misc Feature(10)1059..1061(+)
Misc Feature(11)1209..1211(+)
Misc Feature(12)1266..1268(+)
Misc Feature(13)1275..1277(+)
Misc Feature(14)1326..1328(+)
Misc Feature(15)1329..1331(+)
Misc Feature(16)1335..1337(+)
Misc Feature(17)1341..1343(+)
Misc Feature(18)1377..1379(+)
Exon (1)1..173
Gene:PDPK1
Gene Synonym:
Exon (2)174..434
Gene:PDPK1
Gene Synonym:
Exon (3)435..477
Gene:PDPK1
Gene Synonym:
Exon (4)478..615
Gene:PDPK1
Gene Synonym:
Exon (5)616..760
Gene:PDPK1
Gene Synonym:
Exon (6)761..858
Gene:PDPK1
Gene Synonym:
Exon (7)859..934
Gene:PDPK1
Gene Synonym:
Exon (8)935..1003
Gene:PDPK1
Gene Synonym:
Exon (9)1004..1100
Gene:PDPK1
Gene Synonym:
Exon (10)1101..1274
Gene:PDPK1
Gene Synonym:
Exon (11)1275..1492
Gene:PDPK1
Gene Synonym:
Exon (12)1493..7032
Gene:PDPK1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001261816
Organism Homo sapiens (human)
Definition Homo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001261816

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCCAGGA CCACCAGCCA GCTGTATGAC GCCGTGCCCA TCCAGTCCAG CGTGGTGTTA 
TGTTCCTGCC CATCCCCATC AATGGTGAGG ACCCAGACTG AGTCCAGCAC GCCCCCTGGC
ATTCCTGGTG GCAGCAGGCA GGGCCCCGCC ATGGACGGCA CTGCAGCCGA GCCTCGGCCC
GGCGCCGGCT CCCTGCAGCA TGCCCAGCCT CCGCCGCAGC CTCGGAAGAA GCGGCCTGAG
GACTTCAAGT TTGGGAAAAT CCTTGGGGAA GGCTCTTTTT CCACGGTTGT CCTGGCTCGA
GAACTGGCAA CCTCCAGAGA ATATGCGATT AAAATTCTGG AGAAGCGACA TATCATAAAA
GAGAACAAGG TCCCCTATGT AACCAGAGAG CGGGATGTCA TGTCGCGCCT GGATCACCCC
TTCTTTGTTA AGCTTTACTT CACATTTCAG GACGACGAGA AGCTGTATTT CGGCCTTAGT
TATGCCAAAA ATGGAGAACT ACTTAAATAT ATTCGCAAAA TCGGTTCATT CGATGAGACC
TGTACCCGAT TTTACACGGC TGAGATTGTG TCTGCTTTAG AGTACTTGCA CGGCAAGGGC
ATCATTCACA GGGACCTTAA ACCGGAAAAC ATTTTGTTAA ATGAAGATAT GCACATCCAG
ATCACAGATT TTGGAACAGC AAAAGTCTTA TCCCCAGAGA GCAAACAAGC CAGGGCCAAC
TCATTCGTGG GAACAGCGCA GTACGTTTCT CCAGAGCTGC TCACGGAGAA GTCCGCCTGT
AAGAGTTCAG ACCTTTGGGC TCTTGGATGC ATAATATACC AGCTTGTGGC AGGACTCCCA
CCATTCCGAG CTGGAAACGA GTATCTTATA TTTCAGAAGA TCATTAAGTT GGAATATGAC
TTTCCAGAAA AATTCTTCCC TAAGGCAAGA GACCTCGTGG AGAAACTTTT GGTTTTAGAT
GCCACAAAGC GGTTAGGCTG TGAGGAAATG GAAGGATACG GACCTCTTAA AGCACACCCG
TTCTTCGAGT CCGTCACGTG GGAGAACCTG CACCAGCAGA CGCCTCCGAA GCTCACCGCT
TACCTGCCGG CTATGTCGGA AGACGACGAG GACTGCTATG GCAATTATGA CAATCTCCTG
AGCCAGTTTG GCTGCATGCA GGTGTCTTCG TCCTCCTCCT CACACTCCCT GTCAGCCTCC
GACACGGGCC TGCCCCAGAG GTCAGGCAGC AACATAGAGC AGTACATTCA CGATCTGGAC
TCGAACTCCT TTGAACTGGA CTTACAGTTT TCCGAAGATG AGAAGAGGTT GTTGTTGGAG
AAGCAGGCTG GCGGAAACCC TTGCCTAACA GGACGTATTA TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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