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PEPD cDNA ORF clone, Homo sapiens (human)

Gene Symbol PEPD
Entrez Gene ID 5184
Full Name peptidase D
Synonyms PROLIDASE
General protein information
Preferred Names
xaa-Pro dipeptidase
Names
xaa-Pro dipeptidase
imidodipeptidase
X-Pro dipeptidase
proline dipeptidase
aminoacyl-L-proline hydrolase
NP_000276.2
NP_001159528.1
NP_001159529.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19q13.11

Summary This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]. lac of sum
Disorder MIM:

613230

Disorder Html: Prolidase deficiency, 170100 (3)





The following PEPD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PEPD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu24790 NM_000285 Homo sapiens peptidase D (PEPD), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$265.30
$379.00
OHu24752 NM_001166056 Homo sapiens peptidase D (PEPD), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu25079 NM_001166057 Homo sapiens peptidase D (PEPD), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $359.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu24790
Accession Version NM_000285.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product xaa-Pro dipeptidase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395475.1, BC015027.1 and AW512991.1. This sequence is a reference standard in the RefSeqGene project. On Oct 6, 2009 this sequence version replaced gi:149589007. Summary: This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015027.1, AK225037.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159080 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC
ACCGGGGTCC TCTTCCGCCA GGAGTCCTTC TTTCACTGGG CGTTCGGTGT CACTGAGCCA
GGCTGCTATG GTGTCATCGA TGTTGACACT GGGAAGTCGA CCCTGTTTGT GCCCAGGCTT
CCTGCCAGCC ATGCCACCTG GATGGGAAAG ATCCATTCCA AGGAGCACTT CAAGGAGAAG
TATGCCGTGG ACGACGTCCA GTACGTAGAT GAGATTGCCA GCGTCCTGAC GTCACAGAAG
CCCTCTGTCC TCCTCACTTT GCGTGGCGTC AACACGGACA GCGGCAGTGT CTGCAGGGAG
GCCTCCTTTG ACGGCATCAG CAAGTTCGAA GTCAACAATA CCATTCTTCA CCCAGAGATC
GTTGAGTGCC GAGTGTTTAA GACGGATATG GAGCTGGAGG TTCTGCGCTA TACCAATAAA
ATCTCCAGCG AGGCCCACCG TGAGGTAATG AAGGCTGTAA AAGTGGGAAT GAAAGAATAT
GAGTTGGAAA GCCTCTTCGA GCACTACTGC TACTCCCGGG GCGGCATGCG CCACAGCTCC
TACACCTGCA TCTGCGGCAG TGGTGAGAAC TCAGCCGTGC TACACTACGG ACACGCCGGA
GCTCCCAACG ACCGAACGAT CCAGAATGGG GATATGTGCC TGTTCGACAT GGGCGGTGAG
TATTACTGCT TCGCTTCCGA CATCACCTGC TCCTTTCCCG CCAACGGCAA GTTCACTGCA
GACCAGAAGG CCGTCTATGA GGCAGTGCTG CGGAGCTCCC GTGCCGTCAT GGGTGCCATG
AAGCCAGGTG TCTGGTGGCC TGACATGCAC CGCCTGGCTG ACCGCATCCA CCTGGAGGAG
CTGGCCCACA TGGGCATCCT GAGCGGCAGC GTGGACGCCA TGGTCCAGGC TCACCTGGGG
GCCGTGTTTA TGCCTCACGG GCTTGGCCAC TTCCTGGGCA TTGACGTGCA CGACGTGGGA
GGCTACCCAG AGGGCGTGGA GCGCATCGAC GAGCCCGGCC TGCGGAGCCT GCGCACTGCA
CGGCACCTGC AGCCAGGCAT GGTGCTCACC GTGGAGCCGG GCATCTACTT CATCGACCAC
CTCCTGGATG AGGCCCTGGC GGACCCGGCC CGCGCCTCCT TCCTTAACCG CGAGGTCCTG
CAGCGCTTTC GCGGTTTTGG CGGGGTCCGC ATCGAGGAGG ACGTCGTGGT GACTGACAGC
GGCATAGAGC TGCTGACCTG CGTGCCCCGC ACTGTGGAAG AGATTGAAGC ATGCATGGCA
GGCTGTGACA AGGCCTTTAC CCCCTTCTCT GGCCCCAAGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_000276.2
CDS134..1615
Misc Feature(1)113..115(+)
Misc Feature(2)137..139(+)
Misc Feature(3)185..598(+)
Misc Feature(4)212..1576(+)
Misc Feature(5)707..1534(+)
Misc Feature(6)896..1489(+)
Exon (1)1..150
Gene:PEPD
Gene Synonym:
Exon (2)151..334
Gene:PEPD
Gene Synonym:
Exon (3)335..462
Gene:PEPD
Gene Synonym:
Exon (4)463..526
Gene:PEPD
Gene Synonym:
Exon (5)527..574
Gene:PEPD
Gene Synonym:
Exon (6)575..636
Gene:PEPD
Gene Synonym:
Exon (7)637..681
Gene:PEPD
Gene Synonym:
Exon (8)682..757
Gene:PEPD
Gene Synonym:
Exon (9)758..804
Gene:PEPD
Gene Synonym:
Exon (10)805..873
Gene:PEPD
Gene Synonym:
Exon (11)874..951
Gene:PEPD
Gene Synonym:
Exon (12)952..1100
Gene:PEPD
Gene Synonym:
Exon (13)1101..1285
Gene:PEPD
Gene Synonym:
Exon (14)1286..1477
Gene:PEPD
Gene Synonym:
Exon (15)1478..2010
Gene:PEPD
Gene Synonym:
Translation
Position Chain Variation Link
27 27 a, g dbSNP:528606776
67 67 c, t dbSNP:370425595
96 96 c, t dbSNP:752051747
98 98 a, t dbSNP:764276763
102 102 c, t dbSNP:763089219
103 103 a, g, t dbSNP:566130356
104 104 c, g, t dbSNP:773055268
105 105 a, g dbSNP:772057956
108 108 c, g, t dbSNP:774230871
109 109 c, t dbSNP:768175498
113 113 c, g, t dbSNP:779612612
118 118 c, t dbSNP:755783697
123 123 a, g dbSNP:746272071
129 129 c, t dbSNP:368370973
133 133 c, t dbSNP:757625583
134 134 a, g dbSNP:752145794
135 135 g, t dbSNP:764650519
138 138 c, t dbSNP:758460653
139 139 a, g dbSNP:752794552
140 140 g, t dbSNP:765445780
142 142 g, t dbSNP:759791606
148 148 a, c, t dbSNP:767306239
149 149 a, c, g dbSNP:774320647
152 152 a, c dbSNP:755133215
157 157 a, g dbSNP:374014841
164 164 c, t dbSNP:780319782
165 165 -, g dbSNP:35641847
165 165 c, t dbSNP:756315274
167 167 a, g dbSNP:201992066
175 175 a, g dbSNP:527981355
185 185 a, g dbSNP:758085843
188 188 c, t dbSNP:752700328
189 189 c, t dbSNP:377085952
190 190 a, g dbSNP:759161136
195 195 c, t dbSNP:199711203
196 196 a, g dbSNP:766192958
199 199 c, t dbSNP:760417796
201 201 -, tt dbSNP:754287293
212 212 a, c, t dbSNP:772520514
213 213 a, g dbSNP:372527759
214 214 a, g dbSNP:774960807
219 219 a, g dbSNP:778981204
221 221 c, g dbSNP:749428028
230 230 c, t dbSNP:61734503
231 231 a, g dbSNP:187269138
233 233 c, t dbSNP:376016676
236 236 c, t dbSNP:185183225
237 237 a, g dbSNP:200435937
240 240 a, g dbSNP:752407520
244 244 a, c dbSNP:765289799
259 259 -, c dbSNP:766530739
259 259 c, t dbSNP:754957045
266 266 a, g dbSNP:369878645
268 268 c, t dbSNP:765992641
269 269 a, g dbSNP:779939461
277 277 g, t dbSNP:772942483
283 283 c, t dbSNP:767044241
284 284 a, g dbSNP:762303328
285 285 a, g dbSNP:199892951
296 296 c, t dbSNP:769006603
300 300 a, g dbSNP:749796022
306 306 a, g dbSNP:564126763
310 310 c, g, t dbSNP:372913336
311 311 a, g dbSNP:781520198
316 316 -, g dbSNP:34004641
316 316 c, t dbSNP:560782839
317 317 a, g dbSNP:748037244
320 320 g, t dbSNP:778585918
329 329 c, t dbSNP:368995247
330 330 a, g dbSNP:753859371
333 333 a, g dbSNP:780093052
334 334 a, g dbSNP:755723530
347 347 c, g dbSNP:201752816
348 348 a, g dbSNP:777128955
354 354 c, t dbSNP:533996123
355 355 a, g dbSNP:200108299
358 358 c, g, t dbSNP:538934934
359 359 a, g dbSNP:749151683
366 366 c, g dbSNP:186203899
372 372 c, t dbSNP:769564664
381 381 a, g dbSNP:376397947
391 391 c, t dbSNP:371699437
392 392 a, c, g dbSNP:201865747
404 404 a, g dbSNP:777470431
411 411 c, t dbSNP:375348295
412 412 a, g dbSNP:780889402
416 416 c, t dbSNP:202186183
429 429 g, t dbSNP:755333779
440 440 a, g dbSNP:754327960
443 443 c, t dbSNP:766890433
447 447 c, t dbSNP:760711432
458 458 a, g dbSNP:773410252
464 464 a, g, t dbSNP:755350900
466 466 a, c dbSNP:754202710
468 468 a, g dbSNP:766850315
471 471 c, t dbSNP:570117233
479 479 c, t dbSNP:750890043
484 484 a, c, t dbSNP:374573875
485 485 a, g dbSNP:774763259
492 492 a, g dbSNP:764344730
499 499 a, c, t dbSNP:201145610
500 500 a, g dbSNP:776474982
505 505 c, t dbSNP:371364077
506 506 a, g dbSNP:377429945
508 508 c, t dbSNP:550472850
509 509 a, g dbSNP:770981490
510 510 g, t dbSNP:771596498
512 512 c, t dbSNP:747638822
514 514 g, t dbSNP:778473270
517 517 c, t dbSNP:768352518
518 518 a, g dbSNP:760812846
521 521 g, t dbSNP:533413828
526 526 a, g dbSNP:771409768
531 531 a, c dbSNP:757823745
535 535 c, t dbSNP:373639982
536 536 a, g dbSNP:777913990
543 543 c, t dbSNP:188930796
544 544 a, g dbSNP:370221183
558 558 c, t dbSNP:375915358
560 560 a, g dbSNP:760506591
565 565 c, t dbSNP:750044618
568 568 c, t dbSNP:750217246
575 575 c, t dbSNP:61734505
580 580 c, t dbSNP:375023206
581 581 a, g dbSNP:755007246
585 585 a, c, g dbSNP:368559424
586 586 c, t dbSNP:780223752
587 587 a, g dbSNP:757112748
588 588 a, c, t dbSNP:764150369
589 589 a, g, t dbSNP:200011486
595 595 c, t dbSNP:375631938
596 596 g, t dbSNP:759069264
600 600 c, g dbSNP:753594214
604 604 c, t dbSNP:765917851
605 605 c, t dbSNP:761171509
608 608 a, g dbSNP:773439566
609 609 -, cag dbSNP:769384433
618 618 c, t dbSNP:772426902
619 619 -, t dbSNP:35524700
619 619 c, g dbSNP:576309008
625 625 c, t dbSNP:370105932
627 627 a, g dbSNP:768768872
633 633 a, g dbSNP:61734506
640 640 c, g, t dbSNP:377714630
641 641 a, g dbSNP:758357853
642 642 a, t dbSNP:61748998
644 644 c, g dbSNP:368784737
648 648 a, g dbSNP:764384475
667 667 a, g dbSNP:376361050
669 669 a, c dbSNP:776024032
671 671 a, g dbSNP:765256652
673 673 c, t dbSNP:759640890
674 674 a, c, g dbSNP:372629704
681 681 a, g dbSNP:556625829
683 683 c, t dbSNP:375391662
684 684 a, g dbSNP:121917722
696 696 c, t dbSNP:141623136
697 697 a, g dbSNP:780683391
698 698 a, g dbSNP:756716001
699 699 a, g dbSNP:151278946
719 719 c, t dbSNP:368647287
720 720 a, g dbSNP:374162516
729 729 a, g dbSNP:533937837
738 738 c, t dbSNP:267606943
742 742 c, t dbSNP:575076607
746 746 c, g dbSNP:780447422
747 747 c, t dbSNP:755525017
748 748 c, t dbSNP:754234155
752 752 c, t dbSNP:555350937
753 753 a, g dbSNP:760791817
767 767 g, t dbSNP:747700126
780 780 g, t dbSNP:779343404
790 790 a, g dbSNP:755261014
793 793 c, t dbSNP:3745969
804 804 g, t dbSNP:377738544
806 806 c, t dbSNP:749663409
808 808 a, c, g dbSNP:756699991
811 811 c, t dbSNP:746307467
812 812 a, g dbSNP:539655680
816 816 a, g dbSNP:781362911
819 819 a, g dbSNP:757233987
820 820 c, g dbSNP:369525071
825 825 -, act dbSNP:745834191
825 825 a, g dbSNP:375061486
827 827 a, t dbSNP:751074130
830 830 -, c dbSNP:766829480
830 830 c, t dbSNP:371953949
831 831 a, g dbSNP:754870554
835 835 c, t dbSNP:753617751
836 836 a, g dbSNP:200871513
842 842 c, t dbSNP:766107449
843 843 a, g dbSNP:577079343
845 845 c, t dbSNP:773096608
860 860 c, t dbSNP:767078682
861 861 c, g dbSNP:554131711
868 868 a, c, t dbSNP:199612179
869 869 a, g dbSNP:368547324
873 873 c, g dbSNP:758148942
876 876 a, g dbSNP:775233434
877 877 c, t dbSNP:74988985
884 884 a, t dbSNP:201572375
886 886 a, t dbSNP:190681033
888 888 c, t dbSNP:771330819
889 889 c, t dbSNP:374710449
890 890 a, g dbSNP:200450538
895 895 a, g dbSNP:772101141
900 900 a, g dbSNP:748395241
901 901 c, t dbSNP:149406115
902 902 a, g dbSNP:755947485
905 905 c, g dbSNP:750183982
907 907 c, t dbSNP:559286821
908 908 a, g dbSNP:370370158
910 910 c, t dbSNP:573678038
911 911 a, g dbSNP:763716318
912 912 a, g dbSNP:376338457
917 917 c, t dbSNP:752437700
922 922 a, c dbSNP:764977621
923 923 a, g dbSNP:760037246
926 926 c, g, t dbSNP:121917725
927 927 -, t dbSNP:35226114
927 927 a, g dbSNP:200351927
929 929 a, g dbSNP:773722161
930 930 a, c, g dbSNP:537651471
943 943 a, g dbSNP:79755990
947 947 a, g dbSNP:199794147
952 952 c, t dbSNP:746814800
958 958 -, c dbSNP:750548522
959 959 a, g dbSNP:121917721
961 961 c, t dbSNP:777915295
962 962 a, t dbSNP:374795227
966 966 a, g dbSNP:121917723
967 967 c, t dbSNP:371699300
968 968 a, g dbSNP:754464372
974 974 a, t dbSNP:367902648
975 975 a, g dbSNP:766027294
976 976 c, t dbSNP:756506775
985 985 c, t dbSNP:764699532
986 986 a, g dbSNP:767798921
991 991 c, t dbSNP:375354020
992 992 a, g dbSNP:201584435
994 994 c, t dbSNP:764126413
996 996 c, t dbSNP:139214756
1001 1001 c, t dbSNP:775640182
1003 1003 c, t dbSNP:375514363
1012 1012 c, t dbSNP:773934450
1013 1013 a, g dbSNP:773146135
1018 1018 c, t dbSNP:771953603
1019 1019 a, g dbSNP:370970279
1021 1021 -, gaga dbSNP:767642241
1025 1025 g, t dbSNP:778974892
1034 1034 g, t dbSNP:574398144
1045 1045 -, gcc dbSNP:185359328
1045 1045 c, t dbSNP:199621901
1046 1046 -, ccg dbSNP:762188372
1046 1046 a, g dbSNP:200931112
1050 1050 a, g dbSNP:755808511
1064 1064 c, t dbSNP:750032811
1065 1065 a, g, t dbSNP:1063319
1073 1073 c, t dbSNP:537473674
1074 1074 a, g dbSNP:201447445
1078 1078 c, t dbSNP:529524294
1079 1079 a, g, t dbSNP:370219399
1082 1082 a, g dbSNP:752835320
1087 1087 c, t dbSNP:765164569
1094 1094 a, g dbSNP:759869782
1100 1100 a, c, g dbSNP:375919385
1104 1104 a, t dbSNP:1140312
1106 1106 c, t dbSNP:371556469
1110 1110 a, g dbSNP:529315200
1111 1111 c, g dbSNP:758649991
1117 1117 c, t dbSNP:368959300
1118 1118 a, g dbSNP:563348250
1119 1119 c, t dbSNP:755165324
1124 1124 c, t dbSNP:376817734
1136 1136 c, t dbSNP:543374825
1137 1137 a, g dbSNP:766496514
1149 1149 a, c dbSNP:756414069
1156 1156 a, g dbSNP:750575653
1164 1164 c, t dbSNP:763993962
1177 1177 c, t dbSNP:577733369
1178 1178 a, g dbSNP:765974570
1184 1184 a, g dbSNP:558843453
1189 1189 c, t dbSNP:557804462
1190 1190 a, g dbSNP:759402952
1194 1194 c, t dbSNP:776133687
1195 1195 a, g dbSNP:372210606
1199 1199 a, c dbSNP:746706251
1207 1207 c, t dbSNP:772707771
1212 1212 a, g dbSNP:201089253
1216 1216 c, t dbSNP:201975796
1217 1217 a, g dbSNP:779628053
1222 1222 a, c, t dbSNP:749751344
1227 1227 c, t dbSNP:200183031
1231 1231 c, t dbSNP:149579451
1246 1246 c, g, t dbSNP:767622921
1255 1255 c, t dbSNP:370399053
1258 1258 c, t dbSNP:752440638
1259 1259 a, g dbSNP:374603111
1262 1262 a, c dbSNP:759492742
1263 1263 a, g dbSNP:776511217
1264 1264 c, g, t dbSNP:17569
1265 1265 a, g dbSNP:367841505
1267 1267 c, t dbSNP:761553925
1268 1268 a, g dbSNP:771617102
1279 1279 c, t dbSNP:747766801
1288 1288 c, t dbSNP:763399509
1295 1295 c, t dbSNP:776209368
1296 1296 a, g dbSNP:2230062
1300 1300 c, t dbSNP:560163536
1301 1301 a, g, t dbSNP:377199331
1306 1306 a, g dbSNP:540500595
1309 1309 c, t dbSNP:530300580
1310 1310 a, g dbSNP:777949149
1311 1311 g, t dbSNP:377536201
1317 1317 a, g dbSNP:561611888
1334 1334 c, t dbSNP:753623908
1335 1335 a, g dbSNP:779829444
1337 1337 c, t dbSNP:756015867
1339 1339 c, t dbSNP:750322296
1347 1347 c, g dbSNP:766962619
1353 1353 c, t dbSNP:760080753
1356 1356 c, t dbSNP:761367683
1363 1363 c, t dbSNP:751317868
1364 1364 a, g dbSNP:544921062
1367 1367 a, g dbSNP:267606944
1371 1371 c, t dbSNP:762631029
1372 1372 a, g dbSNP:373060916
1377 1377 a, t dbSNP:575962845
1381 1381 c, t dbSNP:759993012
1382 1382 c, t dbSNP:777165495
1387 1387 c, t dbSNP:770965101
1388 1388 a, c, g dbSNP:559002568
1389 1389 a, g dbSNP:142070498
1390 1390 c, g dbSNP:748309406
1396 1396 c, t dbSNP:779733771
1402 1402 c, t dbSNP:755893460
1413 1413 c, t dbSNP:745829137
1414 1414 a, g dbSNP:183038027
1417 1417 c, t dbSNP:756898414
1424 1424 c, t dbSNP:751121493
1425 1425 a, g dbSNP:763642654
1426 1426 c, t dbSNP:758148546
1427 1427 a, g dbSNP:149042427
1435 1435 -, cct dbSNP:758031251
1436 1436 -, ctt dbSNP:749640022
1436 1436 c, t dbSNP:17570
1437 1437 c, t dbSNP:759922670
1441 1441 c, g, t dbSNP:368792538
1442 1442 c, t dbSNP:376372688
1443 1443 a, g dbSNP:373297406
1444 1444 a, c, t dbSNP:369197590
1445 1445 a, g dbSNP:376211407
1450 1450 c, t dbSNP:369183873
1457 1457 c, t dbSNP:537755422
1458 1458 a, g dbSNP:568774163
1462 1462 c, t dbSNP:2230063
1464 1464 a, g dbSNP:371934154
1466 1466 a, g, t dbSNP:368651528
1472 1472 a, g dbSNP:752342363
1474 1474 c, t dbSNP:764929566
1475 1475 a, g dbSNP:121917724
1483 1483 a, c dbSNP:763268525
1484 1484 a, g dbSNP:377685056
1486 1486 c, t dbSNP:770898814
1487 1487 a, g dbSNP:767937361
1492 1492 -, gga dbSNP:757386104
1492 1492 c, g dbSNP:766389659
1494 1494 a, t dbSNP:773348990
1495 1495 c, t dbSNP:772139535
1496 1496 a, g dbSNP:747974977
1498 1498 c, t dbSNP:778506447
1499 1499 a, g dbSNP:189549581
1500 1500 c, t dbSNP:749007919
1505 1505 a, c dbSNP:779654734
1506 1506 c, t dbSNP:756525693
1507 1507 g, t dbSNP:199999333
1513 1513 c, t dbSNP:781482255
1518 1518 c, t dbSNP:201222933
1520 1520 c, g dbSNP:751974246
1525 1525 g, t dbSNP:758361826
1535 1535 a, g dbSNP:762921626
1541 1541 c, t dbSNP:753026572
1542 1542 a, g dbSNP:765552774
1545 1545 c, t dbSNP:759764278
1546 1546 c, t dbSNP:773154220
1547 1547 a, g dbSNP:200072143
1552 1552 -, a dbSNP:751844685
1553 1553 a, g dbSNP:761839916
1557 1557 c, g, t dbSNP:768258169
1564 1564 a, g dbSNP:765378768
1566 1566 a, c, g dbSNP:372530277
1568 1568 a, g dbSNP:774917395
1575 1575 g, t dbSNP:144944440
1579 1579 c, t dbSNP:745476123
1587 1587 a, c dbSNP:781570190
1588 1588 c, t dbSNP:375224388
1591 1591 c, t dbSNP:776898608
1596 1596 c, g dbSNP:200567073
1603 1603 c, t dbSNP:529823817
1604 1604 a, g dbSNP:758571721
1607 1607 -, ttgg dbSNP:764251321
1608 1608 -, ccaa dbSNP:763184249
1617 1617 g, t dbSNP:567334835
1631 1631 c, t dbSNP:765349872
1635 1635 a, c, t dbSNP:369236384
1636 1636 a, g dbSNP:754071234
1640 1640 c, t dbSNP:767382813
1645 1645 a, g dbSNP:761486668
1653 1653 c, t dbSNP:774402944
1655 1655 a, c, t dbSNP:375067721
1662 1662 c, t dbSNP:775005348
1665 1665 c, t dbSNP:769257563
1666 1666 c, t dbSNP:745563079
1667 1667 c, t dbSNP:1061338
1668 1668 c, t dbSNP:77690463
1669 1669 a, g dbSNP:761144915
1683 1683 a, g dbSNP:552047006
1703 1703 c, t dbSNP:775834584
1729 1729 c, g dbSNP:772651583
1734 1734 g, t dbSNP:762510943
1742 1742 c, t dbSNP:149914845
1770 1770 a, g dbSNP:565481482
1773 1773 a, g dbSNP:762426196
1786 1786 -, ttaa dbSNP:79486519
1790 1790 a, g dbSNP:542575822
1793 1793 -, atta dbSNP:16426
1822 1822 c, g, t dbSNP:1038
1826 1826 c, t dbSNP:3556
1847 1847 c, g dbSNP:34742162
1849 1849 -, g dbSNP:764138313
1859 1859 g, t dbSNP:769512304
1861 1861 -, gat dbSNP:555238351
1861 1861 c, t dbSNP:563417855
1869 1869 a, g dbSNP:140038783
1875 1875 a, g dbSNP:747786921
1881 1881 -, cttt dbSNP:764724976
1922 1922 c, t dbSNP:575087787
1927 1927 c, t dbSNP:558627757
1942 1942 -, tctga dbSNP:201816618
1946 1946 -, tactt dbSNP:398120965
1951 1951 -, tactt dbSNP:10659604
1952 1952 -, acttt dbSNP:140842
1953 1953 -, ct dbSNP:760313240
1993 1993 c, g dbSNP:185823037
2003 2003 a, c dbSNP:560932525

Target ORF information:

RefSeq Version NM_000285
Organism Homo sapiens (human)
Definition Homo sapiens peptidase D (PEPD), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000285

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC
ACCGGGGTCC TCTTCCGCCA GGAGTCCTTC TTTCACTGGG CGTTCGGTGT CACTGAGCCA
GGCTGCTATG GTGTCATCGA TGTTGACACT GGGAAGTCGA CCCTGTTTGT GCCCAGGCTT
CCTGCCAGCC ATGCCACCTG GATGGGAAAG ATCCATTCCA AGGAGCACTT CAAGGAGAAG
TATGCCGTGG ACGACGTCCA GTACGTAGAT GAGATTGCCA GCGTCCTGAC GTCACAGAAG
CCCTCTGTCC TCCTCACTTT GCGTGGCGTC AACACGGACA GCGGCAGTGT CTGCAGGGAG
GCCTCCTTTG ACGGCATCAG CAAGTTCGAA GTCAACAATA CCATTCTTCA CCCAGAGATC
GTTGAGTGCC GAGTGTTTAA GACGGATATG GAGCTGGAGG TTCTGCGCTA TACCAATAAA
ATCTCCAGCG AGGCCCACCG TGAGGTAATG AAGGCTGTAA AAGTGGGAAT GAAAGAATAT
GAGTTGGAAA GCCTCTTCGA GCACTACTGC TACTCCCGGG GCGGCATGCG CCACAGCTCC
TACACCTGCA TCTGCGGCAG TGGTGAGAAC TCAGCCGTGC TACACTACGG ACACGCCGGA
GCTCCCAACG ACCGAACGAT CCAGAATGGG GATATGTGCC TGTTCGACAT GGGCGGTGAG
TATTACTGCT TCGCTTCCGA CATCACCTGC TCCTTTCCCG CCAACGGCAA GTTCACTGCA
GACCAGAAGG CCGTCTATGA GGCAGTGCTG CGGAGCTCCC GTGCCGTCAT GGGTGCCATG
AAGCCAGGTG TCTGGTGGCC TGACATGCAC CGCCTGGCTG ACCGCATCCA CCTGGAGGAG
CTGGCCCACA TGGGCATCCT GAGCGGCAGC GTGGACGCCA TGGTCCAGGC TCACCTGGGG
GCCGTGTTTA TGCCTCACGG GCTTGGCCAC TTCCTGGGCA TTGACGTGCA CGACGTGGGA
GGCTACCCAG AGGGCGTGGA GCGCATCGAC GAGCCCGGCC TGCGGAGCCT GCGCACTGCA
CGGCACCTGC AGCCAGGCAT GGTGCTCACC GTGGAGCCGG GCATCTACTT CATCGACCAC
CTCCTGGATG AGGCCCTGGC GGACCCGGCC CGCGCCTCCT TCCTTAACCG CGAGGTCCTG
CAGCGCTTTC GCGGTTTTGG CGGGGTCCGC ATCGAGGAGG ACGTCGTGGT GACTGACAGC
GGCATAGAGC TGCTGACCTG CGTGCCCCGC ACTGTGGAAG AGATTGAAGC ATGCATGGCA
GGCTGTGACA AGGCCTTTAC CCCCTTCTCT GGCCCCAAGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24752
Accession Version NM_001166056.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1359bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product xaa-Pro dipeptidase isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395475.1, AK294619.1 and AW512991.1. Summary: This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]. Transcript Variant: This variant (2) lacks two alternate in-frame exons in the central coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK294619.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159080 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC
ACCGGGGTCC TCTTCCGCCA GGAGTCCTTC TTTCACTGGG CGTTCGGTGT CACTGAGCCA
GGCTGCTATG GTGTCATCGA TGTTGACACT GGGAAGTCGA CCCTGTTTGT GCCCAGGCTT
CCTGCCAGCC ATGCCACCTG GATGGGAAAG ATCCATTCCA AGGAGCACTT CAAGGAGAAG
TATGCCGTGG ACGACGTCCA GTACGTAGAT GAGATTGCCA GCGTCCTGAC GTCACAGAAG
CCCTCTGTCC TCCTCACTTT GCGTGGCGTC AACACGGACA GCGGCAGTGT CTGCAGGGAG
GCCTCCTTTG ACGGCATCAG CAAGTTCGAA GTCAACAATA CCATTCTTCA CCCAGAGATC
GTTGAGTGCC TCTTCGAGCA CTACTGCTAC TCCCGGGGCG GCATGCGCCA CAGCTCCTAC
ACCTGCATCT GCGGCAGTGG TGAGAACTCA GCCGTGCTAC ACTACGGACA CGCCGGAGCT
CCCAACGACC GAACGATCCA GAATGGGGAT ATGTGCCTGT TCGACATGGG CGGTGAGTAT
TACTGCTTCG CTTCCGACAT CACCTGCTCC TTTCCCGCCA ACGGCAAGTT CACTGCAGAC
CAGAAGGCCG TCTATGAGGC AGTGCTGCGG AGCTCCCGTG CCGTCATGGG TGCCATGAAG
CCAGGTGTCT GGTGGCCTGA CATGCACCGC CTGGCTGACC GCATCCACCT GGAGGAGCTG
GCCCACATGG GCATCCTGAG CGGCAGCGTG GACGCCATGG TCCAGGCTCA CCTGGGGGCC
GTGTTTATGC CTCACGGGCT TGGCCACTTC CTGGGCATTG ACGTGCACGA CGTGGGAGGC
TACCCAGAGG GCGTGGAGCG CATCGACGAG CCCGGCCTGC GGAGCCTGCG CACTGCACGG
CACCTGCAGC CAGGCATGGT GCTCACCGTG GAGCCGGGCA TCTACTTCAT CGACCACCTC
CTGGATGAGG CCCTGGCGGA CCCGGCCCGC GCCTCCTTCC TTAACCGCGA GGTCCTGCAG
CGCTTTCGCG GTTTTGGCGG GGTCCGCATC GAGGAGGACG TCGTGGTGAC TGACAGCGGC
ATAGAGCTGC TGACCTGCGT GCCCCGCACT GTGGAAGAGA TTGAAGCATG CATGGCAGGC
TGTGACAAGG CCTTTACCCC CTTCTCTGGC CCCAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001159528.1
CDS134..1492
Misc Feature(1)113..115(+)
Misc Feature(2)185..541(+)
Misc Feature(3)311..1438(+)
Misc Feature(4)611..1411(+)
Misc Feature(5)773..1366(+)
Exon (1)1..150
Gene:PEPD
Gene Synonym:
Exon (2)151..334
Gene:PEPD
Gene Synonym:
Exon (3)335..462
Gene:PEPD
Gene Synonym:
Exon (4)463..526
Gene:PEPD
Gene Synonym:
Exon (5)527..574
Gene:PEPD
Gene Synonym:
Exon (6)575..636
Gene:PEPD
Gene Synonym:
Exon (7)637..681
Gene:PEPD
Gene Synonym:
Exon (8)682..750
Gene:PEPD
Gene Synonym:
Exon (9)751..828
Gene:PEPD
Gene Synonym:
Exon (10)829..977
Gene:PEPD
Gene Synonym:
Exon (11)978..1162
Gene:PEPD
Gene Synonym:
Exon (12)1163..1354
Gene:PEPD
Gene Synonym:
Exon (13)1355..1887
Gene:PEPD
Gene Synonym:
Translation
Position Chain Variation Link
27 27 a, g dbSNP:528606776
67 67 c, t dbSNP:370425595
96 96 c, t dbSNP:752051747
98 98 a, t dbSNP:764276763
102 102 c, t dbSNP:763089219
103 103 a, g, t dbSNP:566130356
104 104 c, g, t dbSNP:773055268
105 105 a, g dbSNP:772057956
108 108 c, g, t dbSNP:774230871
109 109 c, t dbSNP:768175498
113 113 c, g, t dbSNP:779612612
118 118 c, t dbSNP:755783697
123 123 a, g dbSNP:746272071
129 129 c, t dbSNP:368370973
133 133 c, t dbSNP:757625583
134 134 a, g dbSNP:752145794
135 135 g, t dbSNP:764650519
138 138 c, t dbSNP:758460653
139 139 a, g dbSNP:752794552
140 140 g, t dbSNP:765445780
142 142 g, t dbSNP:759791606
148 148 a, c, t dbSNP:767306239
149 149 a, c, g dbSNP:774320647
152 152 a, c dbSNP:755133215
157 157 a, g dbSNP:374014841
164 164 c, t dbSNP:780319782
165 165 -, g dbSNP:35641847
165 165 c, t dbSNP:756315274
167 167 a, g dbSNP:201992066
175 175 a, g dbSNP:527981355
185 185 a, g dbSNP:758085843
188 188 c, t dbSNP:752700328
189 189 c, t dbSNP:377085952
190 190 a, g dbSNP:759161136
195 195 c, t dbSNP:199711203
196 196 a, g dbSNP:766192958
199 199 c, t dbSNP:760417796
201 201 -, tt dbSNP:754287293
212 212 a, c, t dbSNP:772520514
213 213 a, g dbSNP:372527759
214 214 a, g dbSNP:774960807
219 219 a, g dbSNP:778981204
221 221 c, g dbSNP:749428028
230 230 c, t dbSNP:61734503
231 231 a, g dbSNP:187269138
233 233 c, t dbSNP:376016676
236 236 c, t dbSNP:185183225
237 237 a, g dbSNP:200435937
240 240 a, g dbSNP:752407520
244 244 a, c dbSNP:765289799
259 259 -, c dbSNP:766530739
259 259 c, t dbSNP:754957045
266 266 a, g dbSNP:369878645
268 268 c, t dbSNP:765992641
269 269 a, g dbSNP:779939461
277 277 g, t dbSNP:772942483
283 283 c, t dbSNP:767044241
284 284 a, g dbSNP:762303328
285 285 a, g dbSNP:199892951
296 296 c, t dbSNP:769006603
300 300 a, g dbSNP:749796022
306 306 a, g dbSNP:564126763
310 310 c, g, t dbSNP:372913336
311 311 a, g dbSNP:781520198
316 316 -, g dbSNP:34004641
316 316 c, t dbSNP:560782839
317 317 a, g dbSNP:748037244
320 320 g, t dbSNP:778585918
329 329 c, t dbSNP:368995247
330 330 a, g dbSNP:753859371
333 333 a, g dbSNP:780093052
334 334 a, g dbSNP:755723530
347 347 c, g dbSNP:201752816
348 348 a, g dbSNP:777128955
354 354 c, t dbSNP:533996123
355 355 a, g dbSNP:200108299
358 358 c, g, t dbSNP:538934934
359 359 a, g dbSNP:749151683
366 366 c, g dbSNP:186203899
372 372 c, t dbSNP:769564664
381 381 a, g dbSNP:376397947
391 391 c, t dbSNP:371699437
392 392 a, c, g dbSNP:201865747
404 404 a, g dbSNP:777470431
411 411 c, t dbSNP:375348295
412 412 a, g dbSNP:780889402
416 416 c, t dbSNP:202186183
429 429 g, t dbSNP:755333779
440 440 a, g dbSNP:754327960
443 443 c, t dbSNP:766890433
447 447 c, t dbSNP:760711432
458 458 a, g dbSNP:773410252
464 464 a, g, t dbSNP:755350900
466 466 a, c dbSNP:754202710
468 468 a, g dbSNP:766850315
471 471 c, t dbSNP:570117233
479 479 c, t dbSNP:750890043
484 484 a, c, t dbSNP:374573875
485 485 a, g dbSNP:774763259
492 492 a, g dbSNP:764344730
499 499 a, c, t dbSNP:201145610
500 500 a, g dbSNP:776474982
505 505 c, t dbSNP:371364077
506 506 a, g dbSNP:377429945
508 508 c, t dbSNP:550472850
509 509 a, g dbSNP:770981490
510 510 g, t dbSNP:771596498
512 512 c, t dbSNP:747638822
514 514 g, t dbSNP:778473270
517 517 c, t dbSNP:768352518
518 518 a, g dbSNP:760812846
521 521 g, t dbSNP:533413828
526 526 a, g dbSNP:771409768
531 531 a, c dbSNP:757823745
535 535 c, t dbSNP:373639982
536 536 a, g dbSNP:777913990
543 543 c, t dbSNP:188930796
544 544 a, g dbSNP:370221183
558 558 c, t dbSNP:375915358
560 560 a, g dbSNP:760506591
565 565 c, t dbSNP:750044618
568 568 c, t dbSNP:750217246
575 575 c, t dbSNP:61734505
580 580 c, t dbSNP:375023206
581 581 a, g dbSNP:755007246
585 585 a, c, g dbSNP:368559424
586 586 c, t dbSNP:780223752
587 587 a, g dbSNP:757112748
588 588 a, c, t dbSNP:764150369
589 589 a, g, t dbSNP:200011486
595 595 c, t dbSNP:375631938
596 596 g, t dbSNP:759069264
600 600 c, g dbSNP:753594214
604 604 c, t dbSNP:765917851
605 605 c, t dbSNP:761171509
608 608 a, g dbSNP:773439566
609 609 -, cag dbSNP:769384433
618 618 c, t dbSNP:772426902
619 619 -, t dbSNP:35524700
619 619 c, g dbSNP:576309008
625 625 c, t dbSNP:370105932
627 627 a, g dbSNP:768768872
633 633 a, g dbSNP:61734506
640 640 c, g, t dbSNP:377714630
641 641 a, g dbSNP:758357853
642 642 a, t dbSNP:61748998
644 644 c, g dbSNP:368784737
648 648 a, g dbSNP:764384475
667 667 a, g dbSNP:376361050
669 669 a, c dbSNP:776024032
671 671 a, g dbSNP:765256652
673 673 c, t dbSNP:759640890
674 674 a, c, g dbSNP:372629704
681 681 a, g dbSNP:556625829
683 683 c, t dbSNP:749663409
685 685 a, c, g dbSNP:756699991
688 688 c, t dbSNP:746307467
689 689 a, g dbSNP:539655680
693 693 a, g dbSNP:781362911
696 696 a, g dbSNP:757233987
697 697 c, g dbSNP:369525071
702 702 -, act dbSNP:745834191
702 702 a, g dbSNP:375061486
704 704 a, t dbSNP:751074130
707 707 -, c dbSNP:766829480
707 707 c, t dbSNP:371953949
708 708 a, g dbSNP:754870554
712 712 c, t dbSNP:753617751
713 713 a, g dbSNP:200871513
719 719 c, t dbSNP:766107449
720 720 a, g dbSNP:577079343
722 722 c, t dbSNP:773096608
737 737 c, t dbSNP:767078682
738 738 c, g dbSNP:554131711
745 745 a, c, t dbSNP:199612179
746 746 a, g dbSNP:368547324
750 750 c, g dbSNP:758148942
753 753 a, g dbSNP:775233434
754 754 c, t dbSNP:74988985
761 761 a, t dbSNP:201572375
763 763 a, t dbSNP:190681033
765 765 c, t dbSNP:771330819
766 766 c, t dbSNP:374710449
767 767 a, g dbSNP:200450538
772 772 a, g dbSNP:772101141
777 777 a, g dbSNP:748395241
778 778 c, t dbSNP:149406115
779 779 a, g dbSNP:755947485
782 782 c, g dbSNP:750183982
784 784 c, t dbSNP:559286821
785 785 a, g dbSNP:370370158
787 787 c, t dbSNP:573678038
788 788 a, g dbSNP:763716318
789 789 a, g dbSNP:376338457
794 794 c, t dbSNP:752437700
799 799 a, c dbSNP:764977621
800 800 a, g dbSNP:760037246
803 803 c, g, t dbSNP:121917725
804 804 -, t dbSNP:35226114
804 804 a, g dbSNP:200351927
806 806 a, g dbSNP:773722161
807 807 a, c, g dbSNP:537651471
820 820 a, g dbSNP:79755990
824 824 a, g dbSNP:199794147
829 829 c, t dbSNP:746814800
835 835 -, c dbSNP:750548522
836 836 a, g dbSNP:121917721
838 838 c, t dbSNP:777915295
839 839 a, t dbSNP:374795227
843 843 a, g dbSNP:121917723
844 844 c, t dbSNP:371699300
845 845 a, g dbSNP:754464372
851 851 a, t dbSNP:367902648
852 852 a, g dbSNP:766027294
853 853 c, t dbSNP:756506775
862 862 c, t dbSNP:764699532
863 863 a, g dbSNP:767798921
868 868 c, t dbSNP:375354020
869 869 a, g dbSNP:201584435
871 871 c, t dbSNP:764126413
873 873 c, t dbSNP:139214756
878 878 c, t dbSNP:775640182
880 880 c, t dbSNP:375514363
889 889 c, t dbSNP:773934450
890 890 a, g dbSNP:773146135
895 895 c, t dbSNP:771953603
896 896 a, g dbSNP:370970279
898 898 -, gaga dbSNP:767642241
902 902 g, t dbSNP:778974892
911 911 g, t dbSNP:574398144
922 922 -, gcc dbSNP:185359328
922 922 c, t dbSNP:199621901
923 923 -, ccg dbSNP:762188372
923 923 a, g dbSNP:200931112
927 927 a, g dbSNP:755808511
941 941 c, t dbSNP:750032811
942 942 a, g, t dbSNP:1063319
950 950 c, t dbSNP:537473674
951 951 a, g dbSNP:201447445
955 955 c, t dbSNP:529524294
956 956 a, g, t dbSNP:370219399
959 959 a, g dbSNP:752835320
964 964 c, t dbSNP:765164569
971 971 a, g dbSNP:759869782
977 977 a, c, g dbSNP:375919385
981 981 a, t dbSNP:1140312
983 983 c, t dbSNP:371556469
987 987 a, g dbSNP:529315200
988 988 c, g dbSNP:758649991
994 994 c, t dbSNP:368959300
995 995 a, g dbSNP:563348250
996 996 c, t dbSNP:755165324
1001 1001 c, t dbSNP:376817734
1013 1013 c, t dbSNP:543374825
1014 1014 a, g dbSNP:766496514
1026 1026 a, c dbSNP:756414069
1033 1033 a, g dbSNP:750575653
1041 1041 c, t dbSNP:763993962
1054 1054 c, t dbSNP:577733369
1055 1055 a, g dbSNP:765974570
1061 1061 a, g dbSNP:558843453
1066 1066 c, t dbSNP:557804462
1067 1067 a, g dbSNP:759402952
1071 1071 c, t dbSNP:776133687
1072 1072 a, g dbSNP:372210606
1076 1076 a, c dbSNP:746706251
1084 1084 c, t dbSNP:772707771
1089 1089 a, g dbSNP:201089253
1093 1093 c, t dbSNP:201975796
1094 1094 a, g dbSNP:779628053
1099 1099 a, c, t dbSNP:749751344
1104 1104 c, t dbSNP:200183031
1108 1108 c, t dbSNP:149579451
1123 1123 c, g, t dbSNP:767622921
1132 1132 c, t dbSNP:370399053
1135 1135 c, t dbSNP:752440638
1136 1136 a, g dbSNP:374603111
1139 1139 a, c dbSNP:759492742
1140 1140 a, g dbSNP:776511217
1141 1141 c, g, t dbSNP:17569
1142 1142 a, g dbSNP:367841505
1144 1144 c, t dbSNP:761553925
1145 1145 a, g dbSNP:771617102
1156 1156 c, t dbSNP:747766801
1165 1165 c, t dbSNP:763399509
1172 1172 c, t dbSNP:776209368
1173 1173 a, g dbSNP:2230062
1177 1177 c, t dbSNP:560163536
1178 1178 a, g, t dbSNP:377199331
1183 1183 a, g dbSNP:540500595
1186 1186 c, t dbSNP:530300580
1187 1187 a, g dbSNP:777949149
1188 1188 g, t dbSNP:377536201
1194 1194 a, g dbSNP:561611888
1211 1211 c, t dbSNP:753623908
1212 1212 a, g dbSNP:779829444
1214 1214 c, t dbSNP:756015867
1216 1216 c, t dbSNP:750322296
1224 1224 c, g dbSNP:766962619
1230 1230 c, t dbSNP:760080753
1233 1233 c, t dbSNP:761367683
1240 1240 c, t dbSNP:751317868
1241 1241 a, g dbSNP:544921062
1244 1244 a, g dbSNP:267606944
1248 1248 c, t dbSNP:762631029
1249 1249 a, g dbSNP:373060916
1254 1254 a, t dbSNP:575962845
1258 1258 c, t dbSNP:759993012
1259 1259 c, t dbSNP:777165495
1264 1264 c, t dbSNP:770965101
1265 1265 a, c, g dbSNP:559002568
1266 1266 a, g dbSNP:142070498
1267 1267 c, g dbSNP:748309406
1273 1273 c, t dbSNP:779733771
1279 1279 c, t dbSNP:755893460
1290 1290 c, t dbSNP:745829137
1291 1291 a, g dbSNP:183038027
1294 1294 c, t dbSNP:756898414
1301 1301 c, t dbSNP:751121493
1302 1302 a, g dbSNP:763642654
1303 1303 c, t dbSNP:758148546
1304 1304 a, g dbSNP:149042427
1312 1312 -, cct dbSNP:758031251
1313 1313 -, ctt dbSNP:749640022
1313 1313 c, t dbSNP:17570
1314 1314 c, t dbSNP:759922670
1318 1318 c, g, t dbSNP:368792538
1319 1319 c, t dbSNP:376372688
1320 1320 a, g dbSNP:373297406
1321 1321 a, c, t dbSNP:369197590
1322 1322 a, g dbSNP:376211407
1327 1327 c, t dbSNP:369183873
1334 1334 c, t dbSNP:537755422
1335 1335 a, g dbSNP:568774163
1339 1339 c, t dbSNP:2230063
1341 1341 a, g dbSNP:371934154
1343 1343 a, g, t dbSNP:368651528
1349 1349 a, g dbSNP:752342363
1351 1351 c, t dbSNP:764929566
1352 1352 a, g dbSNP:121917724
1360 1360 a, c dbSNP:763268525
1361 1361 a, g dbSNP:377685056
1363 1363 c, t dbSNP:770898814
1364 1364 a, g dbSNP:767937361
1369 1369 -, gga dbSNP:757386104
1369 1369 c, g dbSNP:766389659
1371 1371 a, t dbSNP:773348990
1372 1372 c, t dbSNP:772139535
1373 1373 a, g dbSNP:747974977
1375 1375 c, t dbSNP:778506447
1376 1376 a, g dbSNP:189549581
1377 1377 c, t dbSNP:749007919
1382 1382 a, c dbSNP:779654734
1383 1383 c, t dbSNP:756525693
1384 1384 g, t dbSNP:199999333
1390 1390 c, t dbSNP:781482255
1395 1395 c, t dbSNP:201222933
1397 1397 c, g dbSNP:751974246
1402 1402 g, t dbSNP:758361826
1412 1412 a, g dbSNP:762921626
1418 1418 c, t dbSNP:753026572
1419 1419 a, g dbSNP:765552774
1422 1422 c, t dbSNP:759764278
1423 1423 c, t dbSNP:773154220
1424 1424 a, g dbSNP:200072143
1429 1429 -, a dbSNP:751844685
1430 1430 a, g dbSNP:761839916
1434 1434 c, g, t dbSNP:768258169
1441 1441 a, g dbSNP:765378768
1443 1443 a, c, g dbSNP:372530277
1445 1445 a, g dbSNP:774917395
1452 1452 g, t dbSNP:144944440
1456 1456 c, t dbSNP:745476123
1464 1464 a, c dbSNP:781570190
1465 1465 c, t dbSNP:375224388
1468 1468 c, t dbSNP:776898608
1473 1473 c, g dbSNP:200567073
1480 1480 c, t dbSNP:529823817
1481 1481 a, g dbSNP:758571721
1484 1484 -, ttgg dbSNP:764251321
1485 1485 -, ccaa dbSNP:763184249
1494 1494 g, t dbSNP:567334835
1508 1508 c, t dbSNP:765349872
1512 1512 a, c, t dbSNP:369236384
1513 1513 a, g dbSNP:754071234
1517 1517 c, t dbSNP:767382813
1522 1522 a, g dbSNP:761486668
1530 1530 c, t dbSNP:774402944
1532 1532 a, c, t dbSNP:375067721
1539 1539 c, t dbSNP:775005348
1542 1542 c, t dbSNP:769257563
1543 1543 c, t dbSNP:745563079
1544 1544 c, t dbSNP:1061338
1545 1545 c, t dbSNP:77690463
1546 1546 a, g dbSNP:761144915
1560 1560 a, g dbSNP:552047006
1580 1580 c, t dbSNP:775834584
1606 1606 c, g dbSNP:772651583
1611 1611 g, t dbSNP:762510943
1619 1619 c, t dbSNP:149914845
1647 1647 a, g dbSNP:565481482
1650 1650 a, g dbSNP:762426196
1663 1663 -, ttaa dbSNP:79486519
1667 1667 a, g dbSNP:542575822
1670 1670 -, atta dbSNP:16426
1699 1699 c, g, t dbSNP:1038
1703 1703 c, t dbSNP:3556
1724 1724 c, g dbSNP:34742162
1726 1726 -, g dbSNP:764138313
1736 1736 g, t dbSNP:769512304
1738 1738 -, gat dbSNP:555238351
1738 1738 c, t dbSNP:563417855
1746 1746 a, g dbSNP:140038783
1752 1752 a, g dbSNP:747786921
1758 1758 -, cttt dbSNP:764724976
1799 1799 c, t dbSNP:575087787
1804 1804 c, t dbSNP:558627757
1819 1819 -, tctga dbSNP:201816618
1823 1823 -, tactt dbSNP:398120965
1828 1828 -, tactt dbSNP:10659604
1829 1829 -, acttt dbSNP:140842
1830 1830 -, ct dbSNP:760313240
1870 1870 c, g dbSNP:185823037
1880 1880 a, c dbSNP:560932525

Target ORF information:

RefSeq Version NM_001166056
Organism Homo sapiens (human)
Definition Homo sapiens peptidase D (PEPD), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001166056

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC
ACCGGGGTCC TCTTCCGCCA GGAGTCCTTC TTTCACTGGG CGTTCGGTGT CACTGAGCCA
GGCTGCTATG GTGTCATCGA TGTTGACACT GGGAAGTCGA CCCTGTTTGT GCCCAGGCTT
CCTGCCAGCC ATGCCACCTG GATGGGAAAG ATCCATTCCA AGGAGCACTT CAAGGAGAAG
TATGCCGTGG ACGACGTCCA GTACGTAGAT GAGATTGCCA GCGTCCTGAC GTCACAGAAG
CCCTCTGTCC TCCTCACTTT GCGTGGCGTC AACACGGACA GCGGCAGTGT CTGCAGGGAG
GCCTCCTTTG ACGGCATCAG CAAGTTCGAA GTCAACAATA CCATTCTTCA CCCAGAGATC
GTTGAGTGCC TCTTCGAGCA CTACTGCTAC TCCCGGGGCG GCATGCGCCA CAGCTCCTAC
ACCTGCATCT GCGGCAGTGG TGAGAACTCA GCCGTGCTAC ACTACGGACA CGCCGGAGCT
CCCAACGACC GAACGATCCA GAATGGGGAT ATGTGCCTGT TCGACATGGG CGGTGAGTAT
TACTGCTTCG CTTCCGACAT CACCTGCTCC TTTCCCGCCA ACGGCAAGTT CACTGCAGAC
CAGAAGGCCG TCTATGAGGC AGTGCTGCGG AGCTCCCGTG CCGTCATGGG TGCCATGAAG
CCAGGTGTCT GGTGGCCTGA CATGCACCGC CTGGCTGACC GCATCCACCT GGAGGAGCTG
GCCCACATGG GCATCCTGAG CGGCAGCGTG GACGCCATGG TCCAGGCTCA CCTGGGGGCC
GTGTTTATGC CTCACGGGCT TGGCCACTTC CTGGGCATTG ACGTGCACGA CGTGGGAGGC
TACCCAGAGG GCGTGGAGCG CATCGACGAG CCCGGCCTGC GGAGCCTGCG CACTGCACGG
CACCTGCAGC CAGGCATGGT GCTCACCGTG GAGCCGGGCA TCTACTTCAT CGACCACCTC
CTGGATGAGG CCCTGGCGGA CCCGGCCCGC GCCTCCTTCC TTAACCGCGA GGTCCTGCAG
CGCTTTCGCG GTTTTGGCGG GGTCCGCATC GAGGAGGACG TCGTGGTGAC TGACAGCGGC
ATAGAGCTGC TGACCTGCGT GCCCCGCACT GTGGAAGAGA TTGAAGCATG CATGGCAGGC
TGTGACAAGG CCTTTACCCC CTTCTCTGGC CCCAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25079
Accession Version NM_001166057.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1290bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product xaa-Pro dipeptidase isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395475.1, AK293126.1, BC015027.1 and AW512991.1. Summary: This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]. Transcript Variant: This variant (3) lacks two alternate in-frame exons in the central coding region, compared to variant 1. The resulting isoform (3) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293126.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC
ACCGGGGTCC TCTTCCGCCA GATTGCCAGC GTCCTGACGT CACAGAAGCC CTCTGTCCTC
CTCACTTTGC GTGGCGTCAA CACGGACAGC GGCAGTGTCT GCAGGGAGGC CTCCTTTGAC
GGCATCAGCA AGTTCGAAGT CAACAATACC ATTCTTCACC CAGAGATCGT TGAGTGCCGA
GTGTTTAAGA CGGATATGGA GCTGGAGGTT CTGCGCTATA CCAATAAAAT CTCCAGCGAG
GCCCACCGTG AGGTAATGAA GGCTGTAAAA GTGGGAATGA AAGAATATGA GTTGGAAAGC
CTCTTCGAGC ACTACTGCTA CTCCCGGGGC GGCATGCGCC ACAGCTCCTA CACCTGCATC
TGCGGCAGTG GTGAGAACTC AGCCGTGCTA CACTACGGAC ACGCCGGAGC TCCCAACGAC
CGAACGATCC AGAATGGGGA TATGTGCCTG TTCGACATGG GCGGTGAGTA TTACTGCTTC
GCTTCCGACA TCACCTGCTC CTTTCCCGCC AACGGCAAGT TCACTGCAGA CCAGAAGGCC
GTCTATGAGG CAGTGCTGCG GAGCTCCCGT GCCGTCATGG GTGCCATGAA GCCAGGTGTC
TGGTGGCCTG ACATGCACCG CCTGGCTGAC CGCATCCACC TGGAGGAGCT GGCCCACATG
GGCATCCTGA GCGGCAGCGT GGACGCCATG GTCCAGGCTC ACCTGGGGGC CGTGTTTATG
CCTCACGGGC TTGGCCACTT CCTGGGCATT GACGTGCACG ACGTGGGAGG CTACCCAGAG
GGCGTGGAGC GCATCGACGA GCCCGGCCTG CGGAGCCTGC GCACTGCACG GCACCTGCAG
CCAGGCATGG TGCTCACCGT GGAGCCGGGC ATCTACTTCA TCGACCACCT CCTGGATGAG
GCCCTGGCGG ACCCGGCCCG CGCCTCCTTC CTTAACCGCG AGGTCCTGCA GCGCTTTCGC
GGTTTTGGCG GGGTCCGCAT CGAGGAGGAC GTCGTGGTGA CTGACAGCGG CATAGAGCTG
CTGACCTGCG TGCCCCGCAC TGTGGAAGAG ATTGAAGCAT GCATGGCAGG CTGTGACAAG
GCCTTTACCC CCTTCTCTGG CCCCAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001159529.1
CDS134..1423
Misc Feature(1)113..115(+)
Misc Feature(2)137..139(+)
Misc Feature(3)185..>334(+)
Misc Feature(4)515..1342(+)
Misc Feature(5)704..1297(+)
Exon (1)1..150
Gene:PEPD
Gene Synonym:
Exon (2)151..334
Gene:PEPD
Gene Synonym:
Exon (3)335..382
Gene:PEPD
Gene Synonym:
Exon (4)383..444
Gene:PEPD
Gene Synonym:
Exon (5)445..489
Gene:PEPD
Gene Synonym:
Exon (6)490..565
Gene:PEPD
Gene Synonym:
Exon (7)566..612
Gene:PEPD
Gene Synonym:
Exon (8)613..681
Gene:PEPD
Gene Synonym:
Exon (9)682..759
Gene:PEPD
Gene Synonym:
Exon (10)760..908
Gene:PEPD
Gene Synonym:
Exon (11)909..1093
Gene:PEPD
Gene Synonym:
Exon (12)1094..1285
Gene:PEPD
Gene Synonym:
Exon (13)1286..1818
Gene:PEPD
Gene Synonym:
Translation
Position Chain Variation Link
27 27 a, g dbSNP:528606776
67 67 c, t dbSNP:370425595
96 96 c, t dbSNP:752051747
98 98 a, t dbSNP:764276763
102 102 c, t dbSNP:763089219
103 103 a, g, t dbSNP:566130356
104 104 c, g, t dbSNP:773055268
105 105 a, g dbSNP:772057956
108 108 c, g, t dbSNP:774230871
109 109 c, t dbSNP:768175498
113 113 c, g, t dbSNP:779612612
118 118 c, t dbSNP:755783697
123 123 a, g dbSNP:746272071
129 129 c, t dbSNP:368370973
133 133 c, t dbSNP:757625583
134 134 a, g dbSNP:752145794
135 135 g, t dbSNP:764650519
138 138 c, t dbSNP:758460653
139 139 a, g dbSNP:752794552
140 140 g, t dbSNP:765445780
142 142 g, t dbSNP:759791606
148 148 a, c, t dbSNP:767306239
149 149 a, c, g dbSNP:774320647
152 152 a, c dbSNP:755133215
157 157 a, g dbSNP:374014841
164 164 c, t dbSNP:780319782
165 165 -, g dbSNP:35641847
165 165 c, t dbSNP:756315274
167 167 a, g dbSNP:201992066
175 175 a, g dbSNP:527981355
185 185 a, g dbSNP:758085843
188 188 c, t dbSNP:752700328
189 189 c, t dbSNP:377085952
190 190 a, g dbSNP:759161136
195 195 c, t dbSNP:199711203
196 196 a, g dbSNP:766192958
199 199 c, t dbSNP:760417796
201 201 -, tt dbSNP:754287293
212 212 a, c, t dbSNP:772520514
213 213 a, g dbSNP:372527759
214 214 a, g dbSNP:774960807
219 219 a, g dbSNP:778981204
221 221 c, g dbSNP:749428028
230 230 c, t dbSNP:61734503
231 231 a, g dbSNP:187269138
233 233 c, t dbSNP:376016676
236 236 c, t dbSNP:185183225
237 237 a, g dbSNP:200435937
240 240 a, g dbSNP:752407520
244 244 a, c dbSNP:765289799
259 259 -, c dbSNP:766530739
259 259 c, t dbSNP:754957045
266 266 a, g dbSNP:369878645
268 268 c, t dbSNP:765992641
269 269 a, g dbSNP:779939461
277 277 g, t dbSNP:772942483
283 283 c, t dbSNP:767044241
284 284 a, g dbSNP:762303328
285 285 a, g dbSNP:199892951
296 296 c, t dbSNP:769006603
300 300 a, g dbSNP:749796022
306 306 a, g dbSNP:564126763
310 310 c, g, t dbSNP:372913336
311 311 a, g dbSNP:781520198
316 316 -, g dbSNP:34004641
316 316 c, t dbSNP:560782839
317 317 a, g dbSNP:748037244
320 320 g, t dbSNP:778585918
329 329 c, t dbSNP:368995247
330 330 a, g dbSNP:753859371
333 333 a, g dbSNP:780093052
334 334 a, g dbSNP:755723530
339 339 a, c dbSNP:757823745
343 343 c, t dbSNP:373639982
344 344 a, g dbSNP:777913990
351 351 c, t dbSNP:188930796
352 352 a, g dbSNP:370221183
366 366 c, t dbSNP:375915358
368 368 a, g dbSNP:760506591
373 373 c, t dbSNP:750044618
376 376 c, t dbSNP:750217246
383 383 c, t dbSNP:61734505
388 388 c, t dbSNP:375023206
389 389 a, g dbSNP:755007246
393 393 a, c, g dbSNP:368559424
394 394 c, t dbSNP:780223752
395 395 a, g dbSNP:757112748
396 396 a, c, t dbSNP:764150369
397 397 a, g, t dbSNP:200011486
403 403 c, t dbSNP:375631938
404 404 g, t dbSNP:759069264
408 408 c, g dbSNP:753594214
412 412 c, t dbSNP:765917851
413 413 c, t dbSNP:761171509
416 416 a, g dbSNP:773439566
417 417 -, cag dbSNP:769384433
426 426 c, t dbSNP:772426902
427 427 -, t dbSNP:35524700
427 427 c, g dbSNP:576309008
433 433 c, t dbSNP:370105932
435 435 a, g dbSNP:768768872
441 441 a, g dbSNP:61734506
448 448 c, g, t dbSNP:377714630
449 449 a, g dbSNP:758357853
450 450 a, t dbSNP:61748998
452 452 c, g dbSNP:368784737
456 456 a, g dbSNP:764384475
475 475 a, g dbSNP:376361050
477 477 a, c dbSNP:776024032
479 479 a, g dbSNP:765256652
481 481 c, t dbSNP:759640890
482 482 a, c, g dbSNP:372629704
489 489 a, g dbSNP:556625829
491 491 c, t dbSNP:375391662
492 492 a, g dbSNP:121917722
504 504 c, t dbSNP:141623136
505 505 a, g dbSNP:780683391
506 506 a, g dbSNP:756716001
507 507 a, g dbSNP:151278946
527 527 c, t dbSNP:368647287
528 528 a, g dbSNP:374162516
537 537 a, g dbSNP:533937837
546 546 c, t dbSNP:267606943
550 550 c, t dbSNP:575076607
554 554 c, g dbSNP:780447422
555 555 c, t dbSNP:755525017
556 556 c, t dbSNP:754234155
560 560 c, t dbSNP:555350937
561 561 a, g dbSNP:760791817
575 575 g, t dbSNP:747700126
588 588 g, t dbSNP:779343404
598 598 a, g dbSNP:755261014
601 601 c, t dbSNP:3745969
612 612 g, t dbSNP:377738544
614 614 c, t dbSNP:749663409
616 616 a, c, g dbSNP:756699991
619 619 c, t dbSNP:746307467
620 620 a, g dbSNP:539655680
624 624 a, g dbSNP:781362911
627 627 a, g dbSNP:757233987
628 628 c, g dbSNP:369525071
633 633 -, act dbSNP:745834191
633 633 a, g dbSNP:375061486
635 635 a, t dbSNP:751074130
638 638 -, c dbSNP:766829480
638 638 c, t dbSNP:371953949
639 639 a, g dbSNP:754870554
643 643 c, t dbSNP:753617751
644 644 a, g dbSNP:200871513
650 650 c, t dbSNP:766107449
651 651 a, g dbSNP:577079343
653 653 c, t dbSNP:773096608
668 668 c, t dbSNP:767078682
669 669 c, g dbSNP:554131711
676 676 a, c, t dbSNP:199612179
677 677 a, g dbSNP:368547324
681 681 c, g dbSNP:758148942
684 684 a, g dbSNP:775233434
685 685 c, t dbSNP:74988985
692 692 a, t dbSNP:201572375
694 694 a, t dbSNP:190681033
696 696 c, t dbSNP:771330819
697 697 c, t dbSNP:374710449
698 698 a, g dbSNP:200450538
703 703 a, g dbSNP:772101141
708 708 a, g dbSNP:748395241
709 709 c, t dbSNP:149406115
710 710 a, g dbSNP:755947485
713 713 c, g dbSNP:750183982
715 715 c, t dbSNP:559286821
716 716 a, g dbSNP:370370158
718 718 c, t dbSNP:573678038
719 719 a, g dbSNP:763716318
720 720 a, g dbSNP:376338457
725 725 c, t dbSNP:752437700
730 730 a, c dbSNP:764977621
731 731 a, g dbSNP:760037246
734 734 c, g, t dbSNP:121917725
735 735 -, t dbSNP:35226114
735 735 a, g dbSNP:200351927
737 737 a, g dbSNP:773722161
738 738 a, c, g dbSNP:537651471
751 751 a, g dbSNP:79755990
755 755 a, g dbSNP:199794147
760 760 c, t dbSNP:746814800
766 766 -, c dbSNP:750548522
767 767 a, g dbSNP:121917721
769 769 c, t dbSNP:777915295
770 770 a, t dbSNP:374795227
774 774 a, g dbSNP:121917723
775 775 c, t dbSNP:371699300
776 776 a, g dbSNP:754464372
782 782 a, t dbSNP:367902648
783 783 a, g dbSNP:766027294
784 784 c, t dbSNP:756506775
793 793 c, t dbSNP:764699532
794 794 a, g dbSNP:767798921
799 799 c, t dbSNP:375354020
800 800 a, g dbSNP:201584435
802 802 c, t dbSNP:764126413
804 804 c, t dbSNP:139214756
809 809 c, t dbSNP:775640182
811 811 c, t dbSNP:375514363
820 820 c, t dbSNP:773934450
821 821 a, g dbSNP:773146135
826 826 c, t dbSNP:771953603
827 827 a, g dbSNP:370970279
829 829 -, gaga dbSNP:767642241
833 833 g, t dbSNP:778974892
842 842 g, t dbSNP:574398144
853 853 -, gcc dbSNP:185359328
853 853 c, t dbSNP:199621901
854 854 -, ccg dbSNP:762188372
854 854 a, g dbSNP:200931112
858 858 a, g dbSNP:755808511
872 872 c, t dbSNP:750032811
873 873 a, g, t dbSNP:1063319
881 881 c, t dbSNP:537473674
882 882 a, g dbSNP:201447445
886 886 c, t dbSNP:529524294
887 887 a, g, t dbSNP:370219399
890 890 a, g dbSNP:752835320
895 895 c, t dbSNP:765164569
902 902 a, g dbSNP:759869782
908 908 a, c, g dbSNP:375919385
912 912 a, t dbSNP:1140312
914 914 c, t dbSNP:371556469
918 918 a, g dbSNP:529315200
919 919 c, g dbSNP:758649991
925 925 c, t dbSNP:368959300
926 926 a, g dbSNP:563348250
927 927 c, t dbSNP:755165324
932 932 c, t dbSNP:376817734
944 944 c, t dbSNP:543374825
945 945 a, g dbSNP:766496514
957 957 a, c dbSNP:756414069
964 964 a, g dbSNP:750575653
972 972 c, t dbSNP:763993962
985 985 c, t dbSNP:577733369
986 986 a, g dbSNP:765974570
992 992 a, g dbSNP:558843453
997 997 c, t dbSNP:557804462
998 998 a, g dbSNP:759402952
1002 1002 c, t dbSNP:776133687
1003 1003 a, g dbSNP:372210606
1007 1007 a, c dbSNP:746706251
1015 1015 c, t dbSNP:772707771
1020 1020 a, g dbSNP:201089253
1024 1024 c, t dbSNP:201975796
1025 1025 a, g dbSNP:779628053
1030 1030 a, c, t dbSNP:749751344
1035 1035 c, t dbSNP:200183031
1039 1039 c, t dbSNP:149579451
1054 1054 c, g, t dbSNP:767622921
1063 1063 c, t dbSNP:370399053
1066 1066 c, t dbSNP:752440638
1067 1067 a, g dbSNP:374603111
1070 1070 a, c dbSNP:759492742
1071 1071 a, g dbSNP:776511217
1072 1072 c, g, t dbSNP:17569
1073 1073 a, g dbSNP:367841505
1075 1075 c, t dbSNP:761553925
1076 1076 a, g dbSNP:771617102
1087 1087 c, t dbSNP:747766801
1096 1096 c, t dbSNP:763399509
1103 1103 c, t dbSNP:776209368
1104 1104 a, g dbSNP:2230062
1108 1108 c, t dbSNP:560163536
1109 1109 a, g, t dbSNP:377199331
1114 1114 a, g dbSNP:540500595
1117 1117 c, t dbSNP:530300580
1118 1118 a, g dbSNP:777949149
1119 1119 g, t dbSNP:377536201
1125 1125 a, g dbSNP:561611888
1142 1142 c, t dbSNP:753623908
1143 1143 a, g dbSNP:779829444
1145 1145 c, t dbSNP:756015867
1147 1147 c, t dbSNP:750322296
1155 1155 c, g dbSNP:766962619
1161 1161 c, t dbSNP:760080753
1164 1164 c, t dbSNP:761367683
1171 1171 c, t dbSNP:751317868
1172 1172 a, g dbSNP:544921062
1175 1175 a, g dbSNP:267606944
1179 1179 c, t dbSNP:762631029
1180 1180 a, g dbSNP:373060916
1185 1185 a, t dbSNP:575962845
1189 1189 c, t dbSNP:759993012
1190 1190 c, t dbSNP:777165495
1195 1195 c, t dbSNP:770965101
1196 1196 a, c, g dbSNP:559002568
1197 1197 a, g dbSNP:142070498
1198 1198 c, g dbSNP:748309406
1204 1204 c, t dbSNP:779733771
1210 1210 c, t dbSNP:755893460
1221 1221 c, t dbSNP:745829137
1222 1222 a, g dbSNP:183038027
1225 1225 c, t dbSNP:756898414
1232 1232 c, t dbSNP:751121493
1233 1233 a, g dbSNP:763642654
1234 1234 c, t dbSNP:758148546
1235 1235 a, g dbSNP:149042427
1243 1243 -, cct dbSNP:758031251
1244 1244 -, ctt dbSNP:749640022
1244 1244 c, t dbSNP:17570
1245 1245 c, t dbSNP:759922670
1249 1249 c, g, t dbSNP:368792538
1250 1250 c, t dbSNP:376372688
1251 1251 a, g dbSNP:373297406
1252 1252 a, c, t dbSNP:369197590
1253 1253 a, g dbSNP:376211407
1258 1258 c, t dbSNP:369183873
1265 1265 c, t dbSNP:537755422
1266 1266 a, g dbSNP:568774163
1270 1270 c, t dbSNP:2230063
1272 1272 a, g dbSNP:371934154
1274 1274 a, g, t dbSNP:368651528
1280 1280 a, g dbSNP:752342363
1282 1282 c, t dbSNP:764929566
1283 1283 a, g dbSNP:121917724
1291 1291 a, c dbSNP:763268525
1292 1292 a, g dbSNP:377685056
1294 1294 c, t dbSNP:770898814
1295 1295 a, g dbSNP:767937361
1300 1300 -, gga dbSNP:757386104
1300 1300 c, g dbSNP:766389659
1302 1302 a, t dbSNP:773348990
1303 1303 c, t dbSNP:772139535
1304 1304 a, g dbSNP:747974977
1306 1306 c, t dbSNP:778506447
1307 1307 a, g dbSNP:189549581
1308 1308 c, t dbSNP:749007919
1313 1313 a, c dbSNP:779654734
1314 1314 c, t dbSNP:756525693
1315 1315 g, t dbSNP:199999333
1321 1321 c, t dbSNP:781482255
1326 1326 c, t dbSNP:201222933
1328 1328 c, g dbSNP:751974246
1333 1333 g, t dbSNP:758361826
1343 1343 a, g dbSNP:762921626
1349 1349 c, t dbSNP:753026572
1350 1350 a, g dbSNP:765552774
1353 1353 c, t dbSNP:759764278
1354 1354 c, t dbSNP:773154220
1355 1355 a, g dbSNP:200072143
1360 1360 -, a dbSNP:751844685
1361 1361 a, g dbSNP:761839916
1365 1365 c, g, t dbSNP:768258169
1372 1372 a, g dbSNP:765378768
1374 1374 a, c, g dbSNP:372530277
1376 1376 a, g dbSNP:774917395
1383 1383 g, t dbSNP:144944440
1387 1387 c, t dbSNP:745476123
1395 1395 a, c dbSNP:781570190
1396 1396 c, t dbSNP:375224388
1399 1399 c, t dbSNP:776898608
1404 1404 c, g dbSNP:200567073
1411 1411 c, t dbSNP:529823817
1412 1412 a, g dbSNP:758571721
1415 1415 -, ttgg dbSNP:764251321
1416 1416 -, ccaa dbSNP:763184249
1425 1425 g, t dbSNP:567334835
1439 1439 c, t dbSNP:765349872
1443 1443 a, c, t dbSNP:369236384
1444 1444 a, g dbSNP:754071234
1448 1448 c, t dbSNP:767382813
1453 1453 a, g dbSNP:761486668
1461 1461 c, t dbSNP:774402944
1463 1463 a, c, t dbSNP:375067721
1470 1470 c, t dbSNP:775005348
1473 1473 c, t dbSNP:769257563
1474 1474 c, t dbSNP:745563079
1475 1475 c, t dbSNP:1061338
1476 1476 c, t dbSNP:77690463
1477 1477 a, g dbSNP:761144915
1491 1491 a, g dbSNP:552047006
1511 1511 c, t dbSNP:775834584
1537 1537 c, g dbSNP:772651583
1542 1542 g, t dbSNP:762510943
1550 1550 c, t dbSNP:149914845
1578 1578 a, g dbSNP:565481482
1581 1581 a, g dbSNP:762426196
1594 1594 -, ttaa dbSNP:79486519
1598 1598 a, g dbSNP:542575822
1601 1601 -, atta dbSNP:16426
1630 1630 c, g, t dbSNP:1038
1634 1634 c, t dbSNP:3556
1655 1655 c, g dbSNP:34742162
1657 1657 -, g dbSNP:764138313
1667 1667 g, t dbSNP:769512304
1669 1669 -, gat dbSNP:555238351
1669 1669 c, t dbSNP:563417855
1677 1677 a, g dbSNP:140038783
1683 1683 a, g dbSNP:747786921
1689 1689 -, cttt dbSNP:764724976
1730 1730 c, t dbSNP:575087787
1735 1735 c, t dbSNP:558627757
1750 1750 -, tctga dbSNP:201816618
1754 1754 -, tactt dbSNP:398120965
1759 1759 -, tactt dbSNP:10659604
1760 1760 -, acttt dbSNP:140842
1761 1761 -, ct dbSNP:760313240
1801 1801 c, g dbSNP:185823037
1811 1811 a, c dbSNP:560932525

Target ORF information:

RefSeq Version NM_001166057
Organism Homo sapiens (human)
Definition Homo sapiens peptidase D (PEPD), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001166057

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC
ACCGGGGTCC TCTTCCGCCA GATTGCCAGC GTCCTGACGT CACAGAAGCC CTCTGTCCTC
CTCACTTTGC GTGGCGTCAA CACGGACAGC GGCAGTGTCT GCAGGGAGGC CTCCTTTGAC
GGCATCAGCA AGTTCGAAGT CAACAATACC ATTCTTCACC CAGAGATCGT TGAGTGCCGA
GTGTTTAAGA CGGATATGGA GCTGGAGGTT CTGCGCTATA CCAATAAAAT CTCCAGCGAG
GCCCACCGTG AGGTAATGAA GGCTGTAAAA GTGGGAATGA AAGAATATGA GTTGGAAAGC
CTCTTCGAGC ACTACTGCTA CTCCCGGGGC GGCATGCGCC ACAGCTCCTA CACCTGCATC
TGCGGCAGTG GTGAGAACTC AGCCGTGCTA CACTACGGAC ACGCCGGAGC TCCCAACGAC
CGAACGATCC AGAATGGGGA TATGTGCCTG TTCGACATGG GCGGTGAGTA TTACTGCTTC
GCTTCCGACA TCACCTGCTC CTTTCCCGCC AACGGCAAGT TCACTGCAGA CCAGAAGGCC
GTCTATGAGG CAGTGCTGCG GAGCTCCCGT GCCGTCATGG GTGCCATGAA GCCAGGTGTC
TGGTGGCCTG ACATGCACCG CCTGGCTGAC CGCATCCACC TGGAGGAGCT GGCCCACATG
GGCATCCTGA GCGGCAGCGT GGACGCCATG GTCCAGGCTC ACCTGGGGGC CGTGTTTATG
CCTCACGGGC TTGGCCACTT CCTGGGCATT GACGTGCACG ACGTGGGAGG CTACCCAGAG
GGCGTGGAGC GCATCGACGA GCCCGGCCTG CGGAGCCTGC GCACTGCACG GCACCTGCAG
CCAGGCATGG TGCTCACCGT GGAGCCGGGC ATCTACTTCA TCGACCACCT CCTGGATGAG
GCCCTGGCGG ACCCGGCCCG CGCCTCCTTC CTTAACCGCG AGGTCCTGCA GCGCTTTCGC
GGTTTTGGCG GGGTCCGCAT CGAGGAGGAC GTCGTGGTGA CTGACAGCGG CATAGAGCTG
CTGACCTGCG TGCCCCGCAC TGTGGAAGAG ATTGAAGCAT GCATGGCAGG CTGTGACAAG
GCCTTTACCC CCTTCTCTGG CCCCAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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