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PFKFB3 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 [Homo sapiens (human)]

Gene Symbol PFKFB3
Entrez Gene ID 5209
Full Name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Synonyms IPFK2, PFK2
General protein information
Preferred Names
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
Names
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
iPFK-2
PFK/FBPase 3
6PF-2-K/Fru-2,6-P2ase 3
renal carcinoma antigen NY-REN-56
6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme
6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase
6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase
fructose-6-phosphate,2-kinase/fructose-2, 6-bisphosphatase
inducible 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
NP_001138915.1
NP_004557.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

10

10p15.1

Summary lac of sum
Disorder MIM:

605319

Disorder Html:
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu43756 XM_005252463 PREDICTED: Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu59628 XM_011519492 PREDICTED: Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu43757 XM_005252464 PREDICTED: Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu59629 XM_011519493 PREDICTED: Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu14576 NM_001282630 Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu15166 NM_004566 Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu14704 NM_001145443 Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu43756D
Sequence Information ORF Nucleotide Sequence (Length: 1545bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008705.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGGG CGTCTGTGAG GAGCTGACCT ACGAGGAGAT CAGGGACACC
TACCCTGAGG AGTATGCGCT GCGGGAGCAG GACAAGTACT ATTACCGCTA CCCCACCGGG
GAGTCCTACC AGGACCTGGT CCAGCGCTTG GAGCCAGTGA TCATGGAGCT GGAGCGGCAG
GAGAATGTGC TGGTCATCTG CCACCAGGCC GTCCTGCGCT GCCTGCTTGC CTACTTCCTG
GATAAGAGTG CAGAGGAGAT GCCCTACCTG AAATGCCCTC TTCACACCGT CCTGAAACTG
ACGCCTGTCG CTTATGGCTG CCGTGTGGAA TCCATCTACC TGAACGTGGA GTCCGTCTGC
ACACACCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCGCTCAT GAGACGCAAT
AGTGTCACCC CGCTAGCCAG CCCCGAACCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG
GAGCATGTGG CCTCCACCTC GGCCGCCCTG CCCAGCTGCC TGCCCCCGGA GGTGCCCACG
CAGCTGCCTG GACAACCTTT GCTAGGGCAA GCCTGTCTAA CATGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005252520.1
CDS385..1929
Misc Feature(1)460..1122(+)
Misc Feature(2)1126..1686(+)
Misc Feature(3)1141..1551(+)
Translation
Position Chain Variation Link
19 19 c, g dbSNP:11257234
35 35 c, t dbSNP:553359827
37 37 a, g dbSNP:11257235
46 46 a, g dbSNP:74379289
65 65 a, g dbSNP:549825839
78 78 a, g dbSNP:560979015
80 80 a, g dbSNP:617245
86 86 a, g dbSNP:543607586
151 151 c, t dbSNP:565058212
163 163 c, g dbSNP:532277271
195 195 a, g dbSNP:547174306
200 200 -, g dbSNP:527630990
203 203 c, t dbSNP:760720377
206 206 c, g dbSNP:557166161
237 237 c, t dbSNP:149331745
245 245 c, t dbSNP:773328449
256 256 c, g dbSNP:764350812
276 276 c, g dbSNP:11596368
291 291 c, g dbSNP:548865023
292 292 -, ct dbSNP:368508609
299 299 c, t dbSNP:570222395
317 317 c, g, t dbSNP:2387004
335 335 a, g dbSNP:202167699
339 339 c, t dbSNP:558760497
342 342 a, g dbSNP:762968358
347 347 c, t dbSNP:369169953
348 348 c, g, t dbSNP:570689483
350 350 c, t dbSNP:767355262
351 351 c, t dbSNP:750229279
361 361 g, t dbSNP:755976157
364 364 c, g dbSNP:376534878
365 365 a, c dbSNP:753338368
368 368 c, t dbSNP:754495467
369 369 -, gg dbSNP:4002912
369 369 a, g dbSNP:553181137
374 374 c, t dbSNP:747812819
379 379 c, g dbSNP:771768885
384 384 c, g dbSNP:370673747
390 390 c, g dbSNP:746264347
397 397 c, g dbSNP:770373341
408 408 c, t dbSNP:368385191
432 432 c, t dbSNP:201238659
433 433 g, t dbSNP:768690057
436 436 a, g dbSNP:774623394
441 441 c, g, t dbSNP:761887123
450 450 c, g dbSNP:750180429
453 453 c, g dbSNP:760486257
458 458 a, c, g dbSNP:200996135
460 460 a, t dbSNP:751708658
461 461 c, t dbSNP:758028195
467 467 a, g dbSNP:763805870
486 486 a, c dbSNP:567913373
487 487 c, t dbSNP:756538480
489 489 a, c dbSNP:528993171
492 492 c, t dbSNP:371698685
493 493 a, g dbSNP:200349885
498 498 c, t dbSNP:779499668
499 499 a, g dbSNP:142603218
516 516 c, t dbSNP:146319524
517 517 a, g dbSNP:759203021
520 520 c, t dbSNP:747176453
546 546 -, gctgactc dbSNP:747356467
552 552 g, t dbSNP:138221143
558 558 c, t dbSNP:776247912
559 559 c, t dbSNP:567196228
564 564 c, t dbSNP:765060646
572 572 a, g dbSNP:775535420
574 574 a, g dbSNP:375018306
576 576 c, t dbSNP:763750903
587 587 c, t dbSNP:781263393
594 594 c, t dbSNP:746156494
595 595 a, g dbSNP:181651478
597 597 -, g dbSNP:780889322
597 597 c, t dbSNP:374201933
610 610 c, t dbSNP:749129357
626 626 a, g dbSNP:547569491
629 629 a, g dbSNP:774481512
631 631 a, g dbSNP:376208847
649 649 c, t dbSNP:771681077
651 651 c, t dbSNP:772727967
655 655 a, g dbSNP:760318737
657 657 c, t dbSNP:199980015
658 658 a, c dbSNP:753009870
664 664 a, g dbSNP:149555675
671 671 c, t dbSNP:764552151
679 679 c, t dbSNP:201889586
680 680 a, g dbSNP:36005730
683 683 a, g dbSNP:757268684
693 693 c, t dbSNP:542439439
695 695 c, t dbSNP:773635607
698 698 c, g dbSNP:760834564
700 700 g, t dbSNP:766625430
715 715 a, c dbSNP:753980509
716 716 a, g dbSNP:765641319
728 728 a, c, t dbSNP:765399775
729 729 a, g dbSNP:750805171
733 733 c, g, t dbSNP:763367903
750 750 a, g dbSNP:757197649
756 756 c, t dbSNP:748952044
770 770 c, t dbSNP:768370594
790 790 a, g dbSNP:777933166
809 809 a, g dbSNP:747409261
822 822 c, t dbSNP:138054261
827 827 c, t dbSNP:144243977
828 828 a, g dbSNP:34969226
837 837 c, t dbSNP:754555836
838 838 a, g dbSNP:773437490
841 841 c, t dbSNP:576701454
842 842 c, t dbSNP:764975677
843 843 a, g dbSNP:79668213
844 844 a, g dbSNP:758136512
845 845 c, t dbSNP:758712431
849 849 c, t dbSNP:746271169
850 850 a, g dbSNP:756527360
852 852 c, t dbSNP:573415324
853 853 a, g dbSNP:749299504
870 870 c, g dbSNP:374769986
877 877 a, g dbSNP:776918283
881 881 c, t dbSNP:774547245
888 888 a, g, t dbSNP:201048797
892 892 a, g dbSNP:373530404
896 896 a, c dbSNP:769828653
913 913 a, g dbSNP:775606147
920 920 a, t dbSNP:762545765
923 923 c, t dbSNP:768274515
924 924 a, g dbSNP:377330736
934 934 a, g dbSNP:761465902
938 938 a, g dbSNP:767107653
939 939 c, t dbSNP:545085751
956 956 a, t dbSNP:759944528
963 963 c, t dbSNP:147548407
969 969 a, c dbSNP:753259168
970 970 a, g dbSNP:758918175
982 982 a, c dbSNP:764167016
987 987 c, t dbSNP:751620453
988 988 a, g dbSNP:757448210
993 993 -, gacaaat dbSNP:765391948
993 993 c, t dbSNP:140074833
994 994 a, g dbSNP:199606482
999 999 a, g dbSNP:756036883
1002 1002 c, g, t dbSNP:780129830
1009 1009 a, g dbSNP:771551601
1016 1016 c, t dbSNP:772721261
1017 1017 a, g, t dbSNP:145567510
1020 1020 a, g dbSNP:558126063
1024 1024 a, c dbSNP:763272837
1031 1031 c, t dbSNP:369033520
1035 1035 c, t dbSNP:150949806
1036 1036 a, g dbSNP:373816623
1042 1042 c, t dbSNP:767650284
1043 1043 a, g dbSNP:140784797
1054 1054 c, g dbSNP:577158958
1061 1061 a, g dbSNP:766735374
1084 1084 c, t dbSNP:753664642
1085 1085 a, g dbSNP:765237719
1086 1086 c, t dbSNP:541321493
1087 1087 a, g dbSNP:752636577
1089 1089 c, t dbSNP:2230271
1090 1090 a, g dbSNP:777223374
1094 1094 a, g dbSNP:746543536
1113 1113 c, t dbSNP:376533039
1114 1114 a, g dbSNP:369124719
1120 1120 c, g dbSNP:749656403
1121 1121 c, t dbSNP:373844823
1122 1122 a, g dbSNP:774789106
1124 1124 a, g dbSNP:762163058
1146 1146 c, t dbSNP:772740743
1147 1147 a, g dbSNP:773379850
1155 1155 c, g, t dbSNP:145448801
1156 1156 a, g dbSNP:574908611
1158 1158 c, g dbSNP:759364786
1160 1160 a, g dbSNP:764872013
1161 1161 c, g dbSNP:752567472
1165 1165 c, g dbSNP:758281350
1174 1174 c, t dbSNP:140258391
1175 1175 a, g, t dbSNP:377041246
1179 1179 a, c, t dbSNP:370128069
1180 1180 a, g dbSNP:34832528
1182 1182 g, t dbSNP:745451556
1185 1185 c, t dbSNP:769493203
1192 1192 a, g dbSNP:12569742
1196 1196 c, t dbSNP:779109196
1202 1202 a, g dbSNP:748570830
1204 1204 a, c, t dbSNP:200723320
1205 1205 a, g dbSNP:200580141
1208 1208 c, g dbSNP:771195007
1212 1212 c, g dbSNP:776787195
1222 1222 a, g dbSNP:145547756
1239 1239 c, t dbSNP:749879794
1240 1240 a, g dbSNP:755690043
1242 1242 a, g dbSNP:765946876
1245 1245 a, g dbSNP:753606554
1264 1264 c, t dbSNP:34714325
1267 1267 c, t dbSNP:372640050
1268 1268 a, g, t dbSNP:748864570
1269 1269 c, t dbSNP:372042331
1270 1270 a, g dbSNP:745927157
1291 1291 a, t dbSNP:769974312
1304 1304 c, t dbSNP:770681879
1305 1305 a, g dbSNP:749444399
1308 1308 c, t dbSNP:768895564
1309 1309 a, g dbSNP:774085700
1313 1313 c, t dbSNP:569471270
1314 1314 a, g dbSNP:138134300
1318 1318 c, t dbSNP:140957456
1319 1319 a, c, g dbSNP:774521447
1329 1329 c, t dbSNP:34184265
1342 1342 a, g dbSNP:753406137
1343 1343 c, t dbSNP:759213296
1344 1344 a, g dbSNP:376217797
1354 1354 a, c dbSNP:751938866
1356 1356 c, t dbSNP:757703975
1357 1357 a, g dbSNP:199705255
1359 1359 c, t dbSNP:750883093
1361 1361 c, t dbSNP:35117690
1362 1362 a, c, g dbSNP:534357183
1365 1365 a, c, t dbSNP:148105023
1366 1366 a, g dbSNP:371075114
1379 1379 g, t dbSNP:762624356
1382 1382 -, cta dbSNP:757624406
1384 1384 g, t dbSNP:763673900
1386 1386 c, t dbSNP:376330766
1387 1387 a, g dbSNP:760987653
1405 1405 a, t dbSNP:766867142
1406 1406 a, g dbSNP:773253990
1417 1417 a, t dbSNP:368231126
1418 1418 a, g, t dbSNP:765237852
1421 1421 c, t dbSNP:141885543
1422 1422 a, g dbSNP:372499415
1426 1426 c, t dbSNP:746812707
1427 1427 a, g dbSNP:757173532
1437 1437 c, t dbSNP:562103199
1444 1444 c, t dbSNP:745733849
1445 1445 a, g dbSNP:769882100
1451 1451 a, g dbSNP:775015647
1452 1452 c, g dbSNP:369242634
1461 1461 c, t dbSNP:574652943
1462 1462 a, g dbSNP:768325811
1464 1464 g, t dbSNP:773944166
1479 1479 c, t dbSNP:762077860
1482 1482 c, g dbSNP:150649154
1489 1489 c, t dbSNP:750211278
1494 1494 a, g dbSNP:756041065
1497 1497 a, g dbSNP:780029811
1498 1498 c, g dbSNP:753660268
1506 1506 a, c, t dbSNP:369277668
1519 1519 c, t dbSNP:759672045
1520 1520 a, g dbSNP:747683414
1526 1526 a, g dbSNP:771692459
1533 1533 a, g dbSNP:2230272
1551 1551 a, g dbSNP:746214599
1554 1554 c, t dbSNP:139855095
1555 1555 a, g dbSNP:775924004
1560 1560 a, g dbSNP:763329061
1562 1562 g, t dbSNP:769227468
1570 1570 c, t dbSNP:774548797
1586 1586 a, t dbSNP:202182009
1591 1591 a, g dbSNP:201735991
1606 1606 a, c dbSNP:756837478
1611 1611 c, t dbSNP:780936222
1614 1614 a, g dbSNP:370388643
1622 1622 a, c dbSNP:755429809
1630 1630 a, g dbSNP:779435896
1632 1632 c, t dbSNP:199594802
1633 1633 a, g dbSNP:143044080
1636 1636 c, t dbSNP:202060851
1646 1646 c, t dbSNP:747166742
1647 1647 a, g, t dbSNP:771030415
1653 1653 c, t dbSNP:749869691
1654 1654 a, g dbSNP:769508911
1666 1666 c, t dbSNP:761670678
1667 1667 a, g dbSNP:766986473
1671 1671 a, g dbSNP:368503704
1677 1677 c, t dbSNP:35050271
1684 1684 c, g dbSNP:760356335
1689 1689 a, c, t dbSNP:140195872
1698 1698 a, c, t dbSNP:780959057
1699 1699 a, g dbSNP:145516111
1701 1701 c, t dbSNP:550940952
1703 1703 g, t dbSNP:778133808
1707 1707 -, a dbSNP:768753859
1710 1710 c, t dbSNP:752034138
1711 1711 c, t dbSNP:144329353
1712 1712 a, g dbSNP:781282147
1716 1716 c, g dbSNP:148777860
1719 1719 a, g dbSNP:745993283
1727 1727 a, g dbSNP:769125713
1736 1736 a, g dbSNP:779620448
1748 1748 c, t dbSNP:143645269
1749 1749 a, g dbSNP:572844646
1750 1750 c, t dbSNP:773544557
1754 1754 c, t dbSNP:761070509
1761 1761 c, g dbSNP:374784976
1764 1764 c, t dbSNP:771424732
1767 1767 c, t dbSNP:777066350
1768 1768 a, g dbSNP:760104468
1771 1771 a, g dbSNP:765168696
1776 1776 a, g dbSNP:35877496
1779 1779 a, g dbSNP:763060765
1788 1788 c, t dbSNP:764446578
1789 1789 a, g dbSNP:368490604
1791 1791 a, g dbSNP:757173698
1805 1805 c, t dbSNP:781147637
1807 1807 c, t dbSNP:750259449
1809 1809 a, c dbSNP:756107683
1810 1810 a, g dbSNP:779383308
1817 1817 a, g dbSNP:748879307
1825 1825 a, g dbSNP:372262915
1827 1827 c, g dbSNP:778605704
1829 1829 a, c, g dbSNP:375524545
1833 1833 g, t dbSNP:371283024
1835 1835 c, t dbSNP:746325286
1838 1838 c, g dbSNP:770377241
1839 1839 c, t dbSNP:775397211
1840 1840 a, g dbSNP:763189277
1842 1842 c, t dbSNP:764395379
1844 1844 c, t dbSNP:143989781
1845 1845 a, g dbSNP:146509899
1848 1848 c, t dbSNP:141132608
1849 1849 a, g, t dbSNP:750254792
1851 1851 c, t dbSNP:766381255
1854 1854 a, g dbSNP:372800366
1858 1858 a, g dbSNP:753284638
1860 1860 c, g dbSNP:754542260
1863 1863 a, c dbSNP:778409661
1864 1864 c, t dbSNP:747734714
1867 1867 a, c, t dbSNP:61731874
1871 1871 c, t dbSNP:746279454
1872 1872 a, g dbSNP:200977986
1877 1877 g, t dbSNP:776120009
1878 1878 a, g dbSNP:749823288
1879 1879 c, g, t dbSNP:768786913
1881 1881 c, t dbSNP:376238290
1883 1883 a, c dbSNP:150176706
1884 1884 a, g dbSNP:201949985
1890 1890 a, g, t dbSNP:760586804
1917 1917 c, t dbSNP:373825024
1918 1918 c, t dbSNP:779603842
1921 1921 c, t dbSNP:751051714
1925 1925 c, t dbSNP:145555537
1931 1931 a, g dbSNP:763945442
1933 1933 g, t dbSNP:35413228
1938 1938 c, t dbSNP:757123518
1939 1939 a, g dbSNP:368989673
1949 1949 c, t dbSNP:749898309
1951 1951 a, t dbSNP:755584243
1955 1955 c, t dbSNP:61731875
1958 1958 c, t dbSNP:748889875
1959 1959 a, g dbSNP:754656515
1962 1962 a, t dbSNP:778078199
1966 1966 a, g dbSNP:747448805
1967 1967 c, t dbSNP:771325150
1969 1969 a, g dbSNP:781754660
1975 1975 g, t dbSNP:765714125
1977 1977 c, g, t dbSNP:373386969
1978 1978 a, g dbSNP:188624660
1982 1982 c, g, t dbSNP:374134958
1983 1983 a, g dbSNP:193259523
1989 1989 a, c, g dbSNP:761737367
2006 2006 c, g dbSNP:762414101
2010 2010 c, t dbSNP:750318120
2012 2012 c, t dbSNP:760551092
2014 2014 a, g dbSNP:201758210
2017 2017 c, t dbSNP:765835251
2018 2018 c, g dbSNP:753384342
2019 2019 c, t dbSNP:754601463
2021 2021 c, t dbSNP:753086468
2031 2031 c, t dbSNP:553362925
2032 2032 a, g dbSNP:3750665
2055 2055 c, g dbSNP:542881129
2062 2062 c, t dbSNP:561001045
2065 2065 c, t dbSNP:751150752
2066 2066 a, g dbSNP:114458714
2092 2092 a, g dbSNP:543203585
2094 2094 c, g dbSNP:564684755
2102 2102 c, t dbSNP:532028772
2103 2103 a, g dbSNP:577319547
2112 2112 c, t dbSNP:546996786
2120 2120 g, t dbSNP:11257460
2133 2133 c, t dbSNP:752489515
2134 2134 c, t dbSNP:760685079
2138 2138 a, c, t dbSNP:370060304
2139 2139 g, t dbSNP:756054427
2163 2163 a, g dbSNP:548899943
2175 2175 c, t dbSNP:373062080
2176 2176 a, g, t dbSNP:111841872
2190 2190 c, t dbSNP:539072183
2200 2200 c, t dbSNP:41291285
2203 2203 c, t dbSNP:570967445
2205 2205 c, t dbSNP:535086675
2210 2210 c, t dbSNP:1539232
2245 2245 c, g, t dbSNP:56656269
2253 2253 a, g dbSNP:555154575
2269 2269 a, g dbSNP:747250693
2273 2273 a, g dbSNP:188911010
2288 2288 a, g dbSNP:1539233
2301 2301 g, t dbSNP:554887905
2372 2372 c, t dbSNP:576254554
2398 2398 c, g dbSNP:766884294
2437 2437 -, c dbSNP:34059583
2454 2454 c, t dbSNP:543213777
2485 2485 c, t dbSNP:564694905
2486 2486 a, g dbSNP:543925758
2511 2511 c, g dbSNP:41291287
2533 2533 c, g dbSNP:149180628
2541 2541 -, c dbSNP:542213461
2542 2542 c, t dbSNP:529736533
2563 2563 a, g dbSNP:11541590
2564 2564 c, t dbSNP:763468630
2592 2592 c, t dbSNP:770357758
2598 2598 a, t dbSNP:115020525
2655 2655 c, g dbSNP:531189229
2667 2667 c, t dbSNP:3750667
2687 2687 a, c, t dbSNP:571030665
2694 2694 g, t dbSNP:759211530
2717 2717 a, c dbSNP:767121904
2719 2719 c, g dbSNP:535210931
2738 2738 c, g dbSNP:75380779
2749 2749 g, t dbSNP:568451349
2761 2761 g, t dbSNP:371109958
2781 2781 c, g dbSNP:752504378
2811 2811 c, g dbSNP:760346345
2814 2814 a, c dbSNP:138719592
2832 2832 c, t dbSNP:576292121
2837 2837 a, g dbSNP:36102318
2841 2841 c, t dbSNP:55643411
2849 2849 c, g dbSNP:3829148
2861 2861 a, g dbSNP:540819800
2862 2862 g, t dbSNP:751642477
2887 2887 c, t dbSNP:181624552
2888 2888 a, g dbSNP:574405068
2924 2924 c, g, t dbSNP:1064887
2963 2963 a, g dbSNP:563104441
2969 2969 c, t dbSNP:116150030
2972 2972 a, t dbSNP:1064888
2973 2973 c, t dbSNP:552934020
2987 2987 c, g dbSNP:564752658
2995 2995 a, g dbSNP:755198570
2999 2999 a, g dbSNP:528734720
3005 3005 c, t dbSNP:546873537
3096 3096 a, g dbSNP:568541518
3100 3100 a, g dbSNP:35781052
3111 3111 a, g dbSNP:146927431
3135 3135 a, g dbSNP:550968611
3174 3174 c, t dbSNP:137871718
3190 3190 c, t dbSNP:183868086
3191 3191 g, t dbSNP:188597321
3229 3229 a, g dbSNP:778111157
3238 3238 a, g dbSNP:576724255
3244 3244 c, g dbSNP:534820299
3294 3294 c, t dbSNP:749787572
3302 3302 g, t dbSNP:552851905
3311 3311 c, t dbSNP:574493771
3321 3321 g, t dbSNP:541534421
3339 3339 c, t dbSNP:563377725
3368 3368 -, att dbSNP:767685301
3406 3406 a, g dbSNP:771483643
3447 3447 a, g dbSNP:1064889
3485 3485 a, g dbSNP:745655832
3544 3544 c, t dbSNP:575348110
3550 3550 c, g dbSNP:546087445
3551 3551 a, g dbSNP:545803813
3554 3554 c, g dbSNP:1064890
3559 3559 c, t dbSNP:373060819
3574 3574 c, t dbSNP:564814115
3575 3575 c, t dbSNP:11541589
3583 3583 a, c dbSNP:561156068
3597 3597 c, t dbSNP:773887335
3598 3598 a, g dbSNP:771717149
3603 3603 c, t dbSNP:528746797
3606 3606 a, g dbSNP:759007137
3615 3615 c, t dbSNP:771642038
3621 3621 c, t dbSNP:540827250
3622 3622 a, g dbSNP:180716582
3623 3623 c, t dbSNP:531498608
3624 3624 a, g dbSNP:113076566
3625 3625 c, t dbSNP:550822376
3627 3627 c, g dbSNP:150577187
3631 3631 -, tc dbSNP:201934407
3639 3639 c, t dbSNP:1064891
3649 3649 c, t dbSNP:574236062
3657 3657 c, t dbSNP:571800119
3674 3674 a, g dbSNP:1064892
3676 3676 g, t dbSNP:551315848
3697 3697 a, g, t dbSNP:111928593
3703 3703 a, g dbSNP:149295239
3759 3759 a, t dbSNP:1064893
3784 3784 c, t dbSNP:186096730
3785 3785 a, g, t dbSNP:3814190
3788 3788 c, g dbSNP:1064894
3805 3805 a, c dbSNP:1064895
3808 3808 a, g dbSNP:1539234
3816 3816 a, t dbSNP:1064896
3830 3830 a, g dbSNP:1064897
3857 3857 c, t dbSNP:547813518
3902 3902 c, t dbSNP:190957966
3909 3909 c, t dbSNP:765521774
3926 3926 a, g dbSNP:373840460
3938 3938 c, t dbSNP:545815893
3949 3949 a, g dbSNP:557742706
3996 3996 c, g dbSNP:573594459
4000 4000 a, c dbSNP:1064898
4020 4020 c, t dbSNP:562383215
4038 4038 c, t dbSNP:565896863
4039 4039 -, ggacggg dbSNP:575355598
4049 4049 a, c dbSNP:113438580
4050 4050 c, t dbSNP:746211569
4064 4064 c, g dbSNP:1064899
4075 4075 -, ac dbSNP:759507402
4077 4077 a, c dbSNP:1064900
4132 4132 c, t dbSNP:773353443
4133 4133 a, g dbSNP:752799904
4138 4138 c, g dbSNP:756391076
4151 4151 c, t dbSNP:529496552
4176 4176 a, t dbSNP:763256173
4179 4179 -, c dbSNP:545922011
4184 4184 -, cc dbSNP:57936464
4186 4186 c, t dbSNP:146257115
4225 4225 c, t dbSNP:562645839
4227 4227 g, t dbSNP:182354027
4279 4279 c, t dbSNP:778092840
4281 4281 c, g dbSNP:551377173
4290 4290 c, t dbSNP:566503745
4308 4308 a, g dbSNP:766589456
4318 4318 g, t dbSNP:186547454
4344 4344 -, g dbSNP:564323726
4419 4419 -, a dbSNP:147522216
4421 4421 c, t dbSNP:372346664
4445 4445 g, t dbSNP:371173465
4456 4456 a, c dbSNP:74795188
4458 4458 a, g dbSNP:536956181
4522 4522 a, t dbSNP:568337048
4547 4547 c, g dbSNP:76767288

Target ORF information:

RefSeq Version XM_005252463
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005252463

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGGG CGTCTGTGAG GAGCTGACCT ACGAGGAGAT CAGGGACACC
TACCCTGAGG AGTATGCGCT GCGGGAGCAG GACAAGTACT ATTACCGCTA CCCCACCGGG
GAGTCCTACC AGGACCTGGT CCAGCGCTTG GAGCCAGTGA TCATGGAGCT GGAGCGGCAG
GAGAATGTGC TGGTCATCTG CCACCAGGCC GTCCTGCGCT GCCTGCTTGC CTACTTCCTG
GATAAGAGTG CAGAGGAGAT GCCCTACCTG AAATGCCCTC TTCACACCGT CCTGAAACTG
ACGCCTGTCG CTTATGGCTG CCGTGTGGAA TCCATCTACC TGAACGTGGA GTCCGTCTGC
ACACACCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCGCTCAT GAGACGCAAT
AGTGTCACCC CGCTAGCCAG CCCCGAACCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG
GAGCATGTGG CCTCCACCTC GGCCGCCCTG CCCAGCTGCC TGCCCCCGGA GGTGCCCACG
CAGCTGCCTG GACAACCTTT GCTAGGGCAA GCCTGTCTAA CATGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu59628D
Sequence Information ORF Nucleotide Sequence (Length: 1458bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008705.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGTC CTACCAGGAC CTGGTCCAGC GCTTGGAGCC AGTGATCATG
GAGCTGGAGC GGCAGGAGAA TGTGCTGGTC ATCTGCCACC AGGCCGTCCT GCGCTGCCTG
CTTGCCTACT TCCTGGATAA GAGTGCAGAG GAGATGCCCT ACCTGAAATG CCCTCTTCAC
ACCGTCCTGA AACTGACGCC TGTCGCTTAT GGCTGCCGTG TGGAATCCAT CTACCTGAAC
GTGGAGTCCG TCTGCACACA CCGGGAGAGG TCAGAGGATG CAAAGAAGGG ACCTAACCCG
CTCATGAGAC GCAATAGTGT CACCCCGCTA GCCAGCCCCG AACCCACCAA AAAGCCTCGC
ATCAACAGCT TTGAGGAGCA TGTGGCCTCC ACCTCGGCCG CCCTGCCCAG CTGCCTGCCC
CCGGAGGTGC CCACGCAGCT GCCTGGACAA AACATGAAAG GCTCCCGGAG CAGCGCTGAC
TCCTCCAGGA AACACTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011517794.1
CDS385..1842
Misc Feature(1)460..1122(+)
Misc Feature(2)1126..1581(+)
Misc Feature(3)1141..1446(+)
Translation
Position Chain Variation Link
19 19 c, g dbSNP:11257234
35 35 c, t dbSNP:553359827
37 37 a, g dbSNP:11257235
46 46 a, g dbSNP:74379289
65 65 a, g dbSNP:549825839
78 78 a, g dbSNP:560979015
80 80 a, g dbSNP:617245
86 86 a, g dbSNP:543607586
151 151 c, t dbSNP:565058212
163 163 c, g dbSNP:532277271
195 195 a, g dbSNP:547174306
200 200 -, g dbSNP:527630990
203 203 c, t dbSNP:760720377
206 206 c, g dbSNP:557166161
237 237 c, t dbSNP:149331745
245 245 c, t dbSNP:773328449
256 256 c, g dbSNP:764350812
276 276 c, g dbSNP:11596368
291 291 c, g dbSNP:548865023
292 292 -, ct dbSNP:368508609
299 299 c, t dbSNP:570222395
317 317 c, g, t dbSNP:2387004
335 335 a, g dbSNP:202167699
339 339 c, t dbSNP:558760497
342 342 a, g dbSNP:762968358
347 347 c, t dbSNP:369169953
348 348 c, g, t dbSNP:570689483
350 350 c, t dbSNP:767355262
351 351 c, t dbSNP:750229279
361 361 g, t dbSNP:755976157
364 364 c, g dbSNP:376534878
365 365 a, c dbSNP:753338368
368 368 c, t dbSNP:754495467
369 369 -, gg dbSNP:4002912
369 369 a, g dbSNP:553181137
374 374 c, t dbSNP:747812819
379 379 c, g dbSNP:771768885
384 384 c, g dbSNP:370673747
390 390 c, g dbSNP:746264347
397 397 c, g dbSNP:770373341
408 408 c, t dbSNP:368385191
432 432 c, t dbSNP:201238659
433 433 g, t dbSNP:768690057
436 436 a, g dbSNP:774623394
441 441 c, g, t dbSNP:761887123
450 450 c, g dbSNP:750180429
453 453 c, g dbSNP:760486257
458 458 a, c, g dbSNP:200996135
460 460 a, t dbSNP:751708658
461 461 c, t dbSNP:758028195
467 467 a, g dbSNP:763805870
486 486 a, c dbSNP:567913373
487 487 c, t dbSNP:756538480
489 489 a, c dbSNP:528993171
492 492 c, t dbSNP:371698685
493 493 a, g dbSNP:200349885
498 498 c, t dbSNP:779499668
499 499 a, g dbSNP:142603218
516 516 c, t dbSNP:146319524
517 517 a, g dbSNP:759203021
520 520 c, t dbSNP:747176453
546 546 -, gctgactc dbSNP:747356467
552 552 g, t dbSNP:138221143
558 558 c, t dbSNP:776247912
559 559 c, t dbSNP:567196228
564 564 c, t dbSNP:765060646
572 572 a, g dbSNP:775535420
574 574 a, g dbSNP:375018306
576 576 c, t dbSNP:763750903
587 587 c, t dbSNP:781263393
594 594 c, t dbSNP:746156494
595 595 a, g dbSNP:181651478
597 597 -, g dbSNP:780889322
597 597 c, t dbSNP:374201933
610 610 c, t dbSNP:749129357
626 626 a, g dbSNP:547569491
629 629 a, g dbSNP:774481512
631 631 a, g dbSNP:376208847
649 649 c, t dbSNP:771681077
651 651 c, t dbSNP:772727967
655 655 a, g dbSNP:760318737
657 657 c, t dbSNP:199980015
658 658 a, c dbSNP:753009870
664 664 a, g dbSNP:149555675
671 671 c, t dbSNP:764552151
679 679 c, t dbSNP:201889586
680 680 a, g dbSNP:36005730
683 683 a, g dbSNP:757268684
693 693 c, t dbSNP:542439439
695 695 c, t dbSNP:773635607
698 698 c, g dbSNP:760834564
700 700 g, t dbSNP:766625430
715 715 a, c dbSNP:753980509
716 716 a, g dbSNP:765641319
728 728 a, c, t dbSNP:765399775
729 729 a, g dbSNP:750805171
733 733 c, g, t dbSNP:763367903
750 750 a, g dbSNP:757197649
756 756 c, t dbSNP:748952044
770 770 c, t dbSNP:768370594
790 790 a, g dbSNP:777933166
809 809 a, g dbSNP:747409261
822 822 c, t dbSNP:138054261
827 827 c, t dbSNP:144243977
828 828 a, g dbSNP:34969226
837 837 c, t dbSNP:754555836
838 838 a, g dbSNP:773437490
841 841 c, t dbSNP:576701454
842 842 c, t dbSNP:764975677
843 843 a, g dbSNP:79668213
844 844 a, g dbSNP:758136512
845 845 c, t dbSNP:758712431
849 849 c, t dbSNP:746271169
850 850 a, g dbSNP:756527360
852 852 c, t dbSNP:573415324
853 853 a, g dbSNP:749299504
870 870 c, g dbSNP:374769986
877 877 a, g dbSNP:776918283
881 881 c, t dbSNP:774547245
888 888 a, g, t dbSNP:201048797
892 892 a, g dbSNP:373530404
896 896 a, c dbSNP:769828653
913 913 a, g dbSNP:775606147
920 920 a, t dbSNP:762545765
923 923 c, t dbSNP:768274515
924 924 a, g dbSNP:377330736
934 934 a, g dbSNP:761465902
938 938 a, g dbSNP:767107653
939 939 c, t dbSNP:545085751
956 956 a, t dbSNP:759944528
963 963 c, t dbSNP:147548407
969 969 a, c dbSNP:753259168
970 970 a, g dbSNP:758918175
982 982 a, c dbSNP:764167016
987 987 c, t dbSNP:751620453
988 988 a, g dbSNP:757448210
993 993 -, gacaaat dbSNP:765391948
993 993 c, t dbSNP:140074833
994 994 a, g dbSNP:199606482
999 999 a, g dbSNP:756036883
1002 1002 c, g, t dbSNP:780129830
1009 1009 a, g dbSNP:771551601
1016 1016 c, t dbSNP:772721261
1017 1017 a, g, t dbSNP:145567510
1020 1020 a, g dbSNP:558126063
1024 1024 a, c dbSNP:763272837
1031 1031 c, t dbSNP:369033520
1035 1035 c, t dbSNP:150949806
1036 1036 a, g dbSNP:373816623
1042 1042 c, t dbSNP:767650284
1043 1043 a, g dbSNP:140784797
1054 1054 c, g dbSNP:577158958
1061 1061 a, g dbSNP:766735374
1084 1084 c, t dbSNP:753664642
1085 1085 a, g dbSNP:765237719
1086 1086 c, t dbSNP:541321493
1087 1087 a, g dbSNP:752636577
1089 1089 c, t dbSNP:2230271
1090 1090 a, g dbSNP:777223374
1094 1094 a, g dbSNP:746543536
1113 1113 c, t dbSNP:376533039
1114 1114 a, g dbSNP:369124719
1120 1120 c, g dbSNP:749656403
1121 1121 c, t dbSNP:373844823
1122 1122 a, g dbSNP:774789106
1124 1124 a, g dbSNP:762163058
1146 1146 c, t dbSNP:772740743
1147 1147 a, g dbSNP:773379850
1155 1155 c, g, t dbSNP:145448801
1156 1156 a, g dbSNP:574908611
1158 1158 c, g dbSNP:759364786
1160 1160 a, g dbSNP:764872013
1161 1161 c, g dbSNP:752567472
1165 1165 c, g dbSNP:758281350
1174 1174 c, t dbSNP:140258391
1175 1175 a, g, t dbSNP:377041246
1179 1179 a, c, t dbSNP:370128069
1180 1180 a, g dbSNP:34832528
1182 1182 g, t dbSNP:745451556
1185 1185 c, t dbSNP:769493203
1192 1192 a, g dbSNP:12569742
1196 1196 c, t dbSNP:779109196
1202 1202 a, g dbSNP:748570830
1204 1204 a, c, t dbSNP:200723320
1205 1205 a, g dbSNP:200580141
1208 1208 c, g dbSNP:771195007
1212 1212 c, g dbSNP:776787195
1222 1222 a, g dbSNP:145547756
1239 1239 c, t dbSNP:749879794
1240 1240 a, g dbSNP:755690043
1242 1242 a, g dbSNP:765946876
1245 1245 a, g dbSNP:753606554
1264 1264 c, t dbSNP:34714325
1267 1267 c, t dbSNP:372640050
1268 1268 a, g, t dbSNP:748864570
1269 1269 c, t dbSNP:372042331
1270 1270 a, g dbSNP:745927157
1291 1291 a, t dbSNP:769974312
1304 1304 c, t dbSNP:770681879
1305 1305 a, g dbSNP:749444399
1308 1308 c, t dbSNP:768895564
1309 1309 a, g dbSNP:774085700
1313 1313 c, t dbSNP:569471270
1314 1314 a, g dbSNP:138134300
1318 1318 c, t dbSNP:140957456
1319 1319 a, c, g dbSNP:774521447
1329 1329 c, t dbSNP:34184265
1342 1342 a, g dbSNP:753406137
1343 1343 c, t dbSNP:759213296
1344 1344 a, g dbSNP:376217797
1354 1354 a, c dbSNP:751938866
1356 1356 c, t dbSNP:757703975
1357 1357 a, g dbSNP:199705255
1359 1359 c, t dbSNP:750883093
1361 1361 c, t dbSNP:35117690
1362 1362 a, c, g dbSNP:534357183
1374 1374 c, t dbSNP:762077860
1377 1377 c, g dbSNP:150649154
1384 1384 c, t dbSNP:750211278
1389 1389 a, g dbSNP:756041065
1392 1392 a, g dbSNP:780029811
1393 1393 c, g dbSNP:753660268
1401 1401 a, c, t dbSNP:369277668
1414 1414 c, t dbSNP:759672045
1415 1415 a, g dbSNP:747683414
1421 1421 a, g dbSNP:771692459
1428 1428 a, g dbSNP:2230272
1446 1446 a, g dbSNP:746214599
1449 1449 c, t dbSNP:139855095
1450 1450 a, g dbSNP:775924004
1455 1455 a, g dbSNP:763329061
1457 1457 g, t dbSNP:769227468
1465 1465 c, t dbSNP:774548797
1481 1481 a, t dbSNP:202182009
1486 1486 a, g dbSNP:201735991
1501 1501 a, c dbSNP:756837478
1506 1506 c, t dbSNP:780936222
1509 1509 a, g dbSNP:370388643
1517 1517 a, c dbSNP:755429809
1525 1525 a, g dbSNP:779435896
1527 1527 c, t dbSNP:199594802
1528 1528 a, g dbSNP:143044080
1531 1531 c, t dbSNP:202060851
1541 1541 c, t dbSNP:747166742
1542 1542 a, g, t dbSNP:771030415
1548 1548 c, t dbSNP:749869691
1549 1549 a, g dbSNP:769508911
1561 1561 c, t dbSNP:761670678
1562 1562 a, g dbSNP:766986473
1566 1566 a, g dbSNP:368503704
1572 1572 c, t dbSNP:35050271
1579 1579 c, g dbSNP:760356335
1584 1584 a, c, t dbSNP:140195872
1593 1593 a, c, t dbSNP:780959057
1594 1594 a, g dbSNP:145516111
1596 1596 c, t dbSNP:550940952
1598 1598 g, t dbSNP:778133808
1602 1602 -, a dbSNP:768753859
1605 1605 c, t dbSNP:752034138
1606 1606 c, t dbSNP:144329353
1607 1607 a, g dbSNP:781282147
1611 1611 c, g dbSNP:148777860
1614 1614 a, g dbSNP:745993283
1622 1622 a, g dbSNP:769125713
1631 1631 a, g dbSNP:779620448
1643 1643 c, t dbSNP:143645269
1644 1644 a, g dbSNP:572844646
1645 1645 c, t dbSNP:773544557
1649 1649 c, t dbSNP:761070509
1656 1656 c, g dbSNP:374784976
1659 1659 c, t dbSNP:771424732
1662 1662 c, t dbSNP:777066350
1663 1663 a, g dbSNP:760104468
1666 1666 a, g dbSNP:765168696
1671 1671 a, g dbSNP:35877496
1674 1674 a, g dbSNP:763060765
1683 1683 c, t dbSNP:764446578
1684 1684 a, g dbSNP:368490604
1686 1686 a, g dbSNP:757173698
1700 1700 c, t dbSNP:781147637
1702 1702 c, t dbSNP:750259449
1704 1704 a, c dbSNP:756107683
1705 1705 a, g dbSNP:779383308
1712 1712 a, g dbSNP:748879307
1720 1720 a, g dbSNP:372262915
1722 1722 c, g dbSNP:778605704
1724 1724 a, c, g dbSNP:375524545
1728 1728 g, t dbSNP:371283024
1730 1730 c, t dbSNP:746325286
1733 1733 c, g dbSNP:770377241
1734 1734 c, t dbSNP:775397211
1735 1735 a, g dbSNP:763189277
1737 1737 c, t dbSNP:764395379
1739 1739 c, t dbSNP:143989781
1740 1740 a, g dbSNP:146509899
1743 1743 c, t dbSNP:141132608
1744 1744 a, g, t dbSNP:750254792
1746 1746 c, t dbSNP:766381255
1749 1749 a, g dbSNP:372800366
1753 1753 a, g dbSNP:753284638
1755 1755 c, g dbSNP:754542260
1758 1758 a, c dbSNP:778409661
1759 1759 c, t dbSNP:747734714
1762 1762 a, c, t dbSNP:61731874
1766 1766 c, t dbSNP:746279454
1767 1767 a, g dbSNP:200977986
1772 1772 g, t dbSNP:776120009
1773 1773 a, g dbSNP:749823288
1774 1774 c, g, t dbSNP:768786913
1776 1776 c, t dbSNP:376238290
1778 1778 a, c dbSNP:150176706
1779 1779 a, g dbSNP:201949985
1785 1785 a, g, t dbSNP:760586804
1797 1797 c, t dbSNP:145555537
1803 1803 a, g dbSNP:763945442
1805 1805 g, t dbSNP:35413228
1810 1810 c, t dbSNP:757123518
1811 1811 a, g dbSNP:368989673
1821 1821 c, t dbSNP:749898309
1823 1823 a, t dbSNP:755584243
1827 1827 c, t dbSNP:61731875
1830 1830 c, t dbSNP:748889875
1831 1831 a, g dbSNP:754656515
1834 1834 a, t dbSNP:778078199
1838 1838 a, g dbSNP:747448805
1839 1839 c, t dbSNP:771325150
1841 1841 a, g dbSNP:781754660
1847 1847 g, t dbSNP:765714125
1849 1849 c, g, t dbSNP:373386969
1850 1850 a, g dbSNP:188624660
1854 1854 c, g, t dbSNP:374134958
1855 1855 a, g dbSNP:193259523
1861 1861 a, c, g dbSNP:761737367
1878 1878 c, g dbSNP:762414101
1882 1882 c, t dbSNP:750318120
1884 1884 c, t dbSNP:760551092
1886 1886 a, g dbSNP:201758210
1889 1889 c, t dbSNP:765835251
1890 1890 c, g dbSNP:753384342
1891 1891 c, t dbSNP:754601463
1893 1893 c, t dbSNP:753086468
1903 1903 c, t dbSNP:553362925
1904 1904 a, g dbSNP:3750665
1927 1927 c, g dbSNP:542881129
1934 1934 c, t dbSNP:561001045
1937 1937 c, t dbSNP:751150752
1938 1938 a, g dbSNP:114458714
1964 1964 a, g dbSNP:543203585
1966 1966 c, g dbSNP:564684755
1974 1974 c, t dbSNP:532028772
1975 1975 a, g dbSNP:577319547
1984 1984 c, t dbSNP:546996786
1992 1992 g, t dbSNP:11257460
2005 2005 c, t dbSNP:752489515
2006 2006 c, t dbSNP:760685079
2010 2010 a, c, t dbSNP:370060304
2011 2011 g, t dbSNP:756054427
2035 2035 a, g dbSNP:548899943
2047 2047 c, t dbSNP:373062080
2048 2048 a, g, t dbSNP:111841872
2062 2062 c, t dbSNP:539072183
2072 2072 c, t dbSNP:41291285
2075 2075 c, t dbSNP:570967445
2077 2077 c, t dbSNP:535086675
2082 2082 c, t dbSNP:1539232
2117 2117 c, g, t dbSNP:56656269
2125 2125 a, g dbSNP:555154575
2141 2141 a, g dbSNP:747250693
2145 2145 a, g dbSNP:188911010
2160 2160 a, g dbSNP:1539233
2173 2173 g, t dbSNP:554887905
2244 2244 c, t dbSNP:576254554
2270 2270 c, g dbSNP:766884294
2309 2309 -, c dbSNP:34059583
2326 2326 c, t dbSNP:543213777
2357 2357 c, t dbSNP:564694905
2358 2358 a, g dbSNP:543925758
2383 2383 c, g dbSNP:41291287
2405 2405 c, g dbSNP:149180628
2413 2413 -, c dbSNP:542213461
2414 2414 c, t dbSNP:529736533
2435 2435 a, g dbSNP:11541590
2436 2436 c, t dbSNP:763468630
2464 2464 c, t dbSNP:770357758
2470 2470 a, t dbSNP:115020525
2527 2527 c, g dbSNP:531189229
2539 2539 c, t dbSNP:3750667
2559 2559 a, c, t dbSNP:571030665
2566 2566 g, t dbSNP:759211530
2589 2589 a, c dbSNP:767121904
2591 2591 c, g dbSNP:535210931
2610 2610 c, g dbSNP:75380779
2621 2621 g, t dbSNP:568451349
2633 2633 g, t dbSNP:371109958
2653 2653 c, g dbSNP:752504378
2683 2683 c, g dbSNP:760346345
2686 2686 a, c dbSNP:138719592
2704 2704 c, t dbSNP:576292121
2709 2709 a, g dbSNP:36102318
2713 2713 c, t dbSNP:55643411
2721 2721 c, g dbSNP:3829148
2733 2733 a, g dbSNP:540819800
2734 2734 g, t dbSNP:751642477
2759 2759 c, t dbSNP:181624552
2760 2760 a, g dbSNP:574405068
2796 2796 c, g, t dbSNP:1064887
2835 2835 a, g dbSNP:563104441
2841 2841 c, t dbSNP:116150030
2844 2844 a, t dbSNP:1064888
2845 2845 c, t dbSNP:552934020
2859 2859 c, g dbSNP:564752658
2867 2867 a, g dbSNP:755198570
2871 2871 a, g dbSNP:528734720
2877 2877 c, t dbSNP:546873537
2968 2968 a, g dbSNP:568541518
2972 2972 a, g dbSNP:35781052
2983 2983 a, g dbSNP:146927431
3007 3007 a, g dbSNP:550968611
3046 3046 c, t dbSNP:137871718
3062 3062 c, t dbSNP:183868086
3063 3063 g, t dbSNP:188597321
3101 3101 a, g dbSNP:778111157
3110 3110 a, g dbSNP:576724255
3116 3116 c, g dbSNP:534820299
3166 3166 c, t dbSNP:749787572
3174 3174 g, t dbSNP:552851905
3183 3183 c, t dbSNP:574493771
3193 3193 g, t dbSNP:541534421
3211 3211 c, t dbSNP:563377725
3240 3240 -, att dbSNP:767685301
3278 3278 a, g dbSNP:771483643
3319 3319 a, g dbSNP:1064889
3357 3357 a, g dbSNP:745655832
3416 3416 c, t dbSNP:575348110
3422 3422 c, g dbSNP:546087445
3423 3423 a, g dbSNP:545803813
3426 3426 c, g dbSNP:1064890
3431 3431 c, t dbSNP:373060819
3446 3446 c, t dbSNP:564814115
3447 3447 c, t dbSNP:11541589
3455 3455 a, c dbSNP:561156068
3469 3469 c, t dbSNP:773887335
3470 3470 a, g dbSNP:771717149
3475 3475 c, t dbSNP:528746797
3478 3478 a, g dbSNP:759007137
3487 3487 c, t dbSNP:771642038
3493 3493 c, t dbSNP:540827250
3494 3494 a, g dbSNP:180716582
3495 3495 c, t dbSNP:531498608
3496 3496 a, g dbSNP:113076566
3497 3497 c, t dbSNP:550822376
3499 3499 c, g dbSNP:150577187
3503 3503 -, tc dbSNP:201934407
3511 3511 c, t dbSNP:1064891
3521 3521 c, t dbSNP:574236062
3529 3529 c, t dbSNP:571800119
3546 3546 a, g dbSNP:1064892
3548 3548 g, t dbSNP:551315848
3569 3569 a, g, t dbSNP:111928593
3575 3575 a, g dbSNP:149295239
3631 3631 a, t dbSNP:1064893
3656 3656 c, t dbSNP:186096730
3657 3657 a, g, t dbSNP:3814190
3660 3660 c, g dbSNP:1064894
3677 3677 a, c dbSNP:1064895
3680 3680 a, g dbSNP:1539234
3688 3688 a, t dbSNP:1064896
3702 3702 a, g dbSNP:1064897
3729 3729 c, t dbSNP:547813518
3774 3774 c, t dbSNP:190957966
3781 3781 c, t dbSNP:765521774
3798 3798 a, g dbSNP:373840460
3810 3810 c, t dbSNP:545815893
3821 3821 a, g dbSNP:557742706
3868 3868 c, g dbSNP:573594459
3872 3872 a, c dbSNP:1064898
3892 3892 c, t dbSNP:562383215
3910 3910 c, t dbSNP:565896863
3911 3911 -, ggacggg dbSNP:575355598
3921 3921 a, c dbSNP:113438580
3922 3922 c, t dbSNP:746211569
3936 3936 c, g dbSNP:1064899
3947 3947 -, ac dbSNP:759507402
3949 3949 a, c dbSNP:1064900
4004 4004 c, t dbSNP:773353443
4005 4005 a, g dbSNP:752799904
4010 4010 c, g dbSNP:756391076
4023 4023 c, t dbSNP:529496552
4048 4048 a, t dbSNP:763256173
4051 4051 -, c dbSNP:545922011
4056 4056 -, cc dbSNP:57936464
4058 4058 c, t dbSNP:146257115
4097 4097 c, t dbSNP:562645839
4099 4099 g, t dbSNP:182354027
4151 4151 c, t dbSNP:778092840
4153 4153 c, g dbSNP:551377173
4162 4162 c, t dbSNP:566503745
4180 4180 a, g dbSNP:766589456
4190 4190 g, t dbSNP:186547454
4216 4216 -, g dbSNP:564323726
4291 4291 -, a dbSNP:147522216
4293 4293 c, t dbSNP:372346664
4317 4317 g, t dbSNP:371173465
4328 4328 a, c dbSNP:74795188
4330 4330 a, g dbSNP:536956181
4394 4394 a, t dbSNP:568337048
4419 4419 c, g dbSNP:76767288

Target ORF information:

RefSeq Version XM_011519492
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011519492

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGTC CTACCAGGAC CTGGTCCAGC GCTTGGAGCC AGTGATCATG
GAGCTGGAGC GGCAGGAGAA TGTGCTGGTC ATCTGCCACC AGGCCGTCCT GCGCTGCCTG
CTTGCCTACT TCCTGGATAA GAGTGCAGAG GAGATGCCCT ACCTGAAATG CCCTCTTCAC
ACCGTCCTGA AACTGACGCC TGTCGCTTAT GGCTGCCGTG TGGAATCCAT CTACCTGAAC
GTGGAGTCCG TCTGCACACA CCGGGAGAGG TCAGAGGATG CAAAGAAGGG ACCTAACCCG
CTCATGAGAC GCAATAGTGT CACCCCGCTA GCCAGCCCCG AACCCACCAA AAAGCCTCGC
ATCAACAGCT TTGAGGAGCA TGTGGCCTCC ACCTCGGCCG CCCTGCCCAG CTGCCTGCCC
CCGGAGGTGC CCACGCAGCT GCCTGGACAA AACATGAAAG GCTCCCGGAG CAGCGCTGAC
TCCTCCAGGA AACACTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu43757D
Sequence Information ORF Nucleotide Sequence (Length: 1389bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008705.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGGG CGTCTGTGAG GAGCTGACCT ACGAGGAGAT CAGGGACACC
TACCCTGAGG AGTATGCGCT GCGGGAGCAG GACAAGTACT ATTACCGCTA CCCCACCGGG
GAGTCCTACC AGGACCTGGT CCAGCGCTTG GAGCCAGTGA TCATGGAGCT GGAGCGGCAG
GAGAATGTGC TGGTCATCTG CCACCAGGCC GTCCTGCGCT GCCTGCTTGC CTACTTCCTG
GATAAGAGTG CAGAGGAGAT GCCCTACCTG AAATGCCCTC TTCACACCGT CCTGAAACTG
ACGCCTGTCG CTTATGGCTG CCGTGTGGAA TCCATCTACC TGAACGTGGA GTCCGTCTGC
ACACACCGGG AGAGGTCAGA GAACATGAAA GGCTCCCGGA GCAGCGCTGA CTCCTCCAGG
AAACACTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005252521.1
CDS385..1773
Misc Feature(1)460..1122(+)
Misc Feature(2)1126..1686(+)
Misc Feature(3)1141..1551(+)
Translation
Position Chain Variation Link
19 19 c, g dbSNP:11257234
35 35 c, t dbSNP:553359827
37 37 a, g dbSNP:11257235
46 46 a, g dbSNP:74379289
65 65 a, g dbSNP:549825839
78 78 a, g dbSNP:560979015
80 80 a, g dbSNP:617245
86 86 a, g dbSNP:543607586
151 151 c, t dbSNP:565058212
163 163 c, g dbSNP:532277271
195 195 a, g dbSNP:547174306
200 200 -, g dbSNP:527630990
203 203 c, t dbSNP:760720377
206 206 c, g dbSNP:557166161
237 237 c, t dbSNP:149331745
245 245 c, t dbSNP:773328449
256 256 c, g dbSNP:764350812
276 276 c, g dbSNP:11596368
291 291 c, g dbSNP:548865023
292 292 -, ct dbSNP:368508609
299 299 c, t dbSNP:570222395
317 317 c, g, t dbSNP:2387004
335 335 a, g dbSNP:202167699
339 339 c, t dbSNP:558760497
342 342 a, g dbSNP:762968358
347 347 c, t dbSNP:369169953
348 348 c, g, t dbSNP:570689483
350 350 c, t dbSNP:767355262
351 351 c, t dbSNP:750229279
361 361 g, t dbSNP:755976157
364 364 c, g dbSNP:376534878
365 365 a, c dbSNP:753338368
368 368 c, t dbSNP:754495467
369 369 -, gg dbSNP:4002912
369 369 a, g dbSNP:553181137
374 374 c, t dbSNP:747812819
379 379 c, g dbSNP:771768885
384 384 c, g dbSNP:370673747
390 390 c, g dbSNP:746264347
397 397 c, g dbSNP:770373341
408 408 c, t dbSNP:368385191
432 432 c, t dbSNP:201238659
433 433 g, t dbSNP:768690057
436 436 a, g dbSNP:774623394
441 441 c, g, t dbSNP:761887123
450 450 c, g dbSNP:750180429
453 453 c, g dbSNP:760486257
458 458 a, c, g dbSNP:200996135
460 460 a, t dbSNP:751708658
461 461 c, t dbSNP:758028195
467 467 a, g dbSNP:763805870
486 486 a, c dbSNP:567913373
487 487 c, t dbSNP:756538480
489 489 a, c dbSNP:528993171
492 492 c, t dbSNP:371698685
493 493 a, g dbSNP:200349885
498 498 c, t dbSNP:779499668
499 499 a, g dbSNP:142603218
516 516 c, t dbSNP:146319524
517 517 a, g dbSNP:759203021
520 520 c, t dbSNP:747176453
546 546 -, gctgactc dbSNP:747356467
552 552 g, t dbSNP:138221143
558 558 c, t dbSNP:776247912
559 559 c, t dbSNP:567196228
564 564 c, t dbSNP:765060646
572 572 a, g dbSNP:775535420
574 574 a, g dbSNP:375018306
576 576 c, t dbSNP:763750903
587 587 c, t dbSNP:781263393
594 594 c, t dbSNP:746156494
595 595 a, g dbSNP:181651478
597 597 -, g dbSNP:780889322
597 597 c, t dbSNP:374201933
610 610 c, t dbSNP:749129357
626 626 a, g dbSNP:547569491
629 629 a, g dbSNP:774481512
631 631 a, g dbSNP:376208847
649 649 c, t dbSNP:771681077
651 651 c, t dbSNP:772727967
655 655 a, g dbSNP:760318737
657 657 c, t dbSNP:199980015
658 658 a, c dbSNP:753009870
664 664 a, g dbSNP:149555675
671 671 c, t dbSNP:764552151
679 679 c, t dbSNP:201889586
680 680 a, g dbSNP:36005730
683 683 a, g dbSNP:757268684
693 693 c, t dbSNP:542439439
695 695 c, t dbSNP:773635607
698 698 c, g dbSNP:760834564
700 700 g, t dbSNP:766625430
715 715 a, c dbSNP:753980509
716 716 a, g dbSNP:765641319
728 728 a, c, t dbSNP:765399775
729 729 a, g dbSNP:750805171
733 733 c, g, t dbSNP:763367903
750 750 a, g dbSNP:757197649
756 756 c, t dbSNP:748952044
770 770 c, t dbSNP:768370594
790 790 a, g dbSNP:777933166
809 809 a, g dbSNP:747409261
822 822 c, t dbSNP:138054261
827 827 c, t dbSNP:144243977
828 828 a, g dbSNP:34969226
837 837 c, t dbSNP:754555836
838 838 a, g dbSNP:773437490
841 841 c, t dbSNP:576701454
842 842 c, t dbSNP:764975677
843 843 a, g dbSNP:79668213
844 844 a, g dbSNP:758136512
845 845 c, t dbSNP:758712431
849 849 c, t dbSNP:746271169
850 850 a, g dbSNP:756527360
852 852 c, t dbSNP:573415324
853 853 a, g dbSNP:749299504
870 870 c, g dbSNP:374769986
877 877 a, g dbSNP:776918283
881 881 c, t dbSNP:774547245
888 888 a, g, t dbSNP:201048797
892 892 a, g dbSNP:373530404
896 896 a, c dbSNP:769828653
913 913 a, g dbSNP:775606147
920 920 a, t dbSNP:762545765
923 923 c, t dbSNP:768274515
924 924 a, g dbSNP:377330736
934 934 a, g dbSNP:761465902
938 938 a, g dbSNP:767107653
939 939 c, t dbSNP:545085751
956 956 a, t dbSNP:759944528
963 963 c, t dbSNP:147548407
969 969 a, c dbSNP:753259168
970 970 a, g dbSNP:758918175
982 982 a, c dbSNP:764167016
987 987 c, t dbSNP:751620453
988 988 a, g dbSNP:757448210
993 993 -, gacaaat dbSNP:765391948
993 993 c, t dbSNP:140074833
994 994 a, g dbSNP:199606482
999 999 a, g dbSNP:756036883
1002 1002 c, g, t dbSNP:780129830
1009 1009 a, g dbSNP:771551601
1016 1016 c, t dbSNP:772721261
1017 1017 a, g, t dbSNP:145567510
1020 1020 a, g dbSNP:558126063
1024 1024 a, c dbSNP:763272837
1031 1031 c, t dbSNP:369033520
1035 1035 c, t dbSNP:150949806
1036 1036 a, g dbSNP:373816623
1042 1042 c, t dbSNP:767650284
1043 1043 a, g dbSNP:140784797
1054 1054 c, g dbSNP:577158958
1061 1061 a, g dbSNP:766735374
1084 1084 c, t dbSNP:753664642
1085 1085 a, g dbSNP:765237719
1086 1086 c, t dbSNP:541321493
1087 1087 a, g dbSNP:752636577
1089 1089 c, t dbSNP:2230271
1090 1090 a, g dbSNP:777223374
1094 1094 a, g dbSNP:746543536
1113 1113 c, t dbSNP:376533039
1114 1114 a, g dbSNP:369124719
1120 1120 c, g dbSNP:749656403
1121 1121 c, t dbSNP:373844823
1122 1122 a, g dbSNP:774789106
1124 1124 a, g dbSNP:762163058
1146 1146 c, t dbSNP:772740743
1147 1147 a, g dbSNP:773379850
1155 1155 c, g, t dbSNP:145448801
1156 1156 a, g dbSNP:574908611
1158 1158 c, g dbSNP:759364786
1160 1160 a, g dbSNP:764872013
1161 1161 c, g dbSNP:752567472
1165 1165 c, g dbSNP:758281350
1174 1174 c, t dbSNP:140258391
1175 1175 a, g, t dbSNP:377041246
1179 1179 a, c, t dbSNP:370128069
1180 1180 a, g dbSNP:34832528
1182 1182 g, t dbSNP:745451556
1185 1185 c, t dbSNP:769493203
1192 1192 a, g dbSNP:12569742
1196 1196 c, t dbSNP:779109196
1202 1202 a, g dbSNP:748570830
1204 1204 a, c, t dbSNP:200723320
1205 1205 a, g dbSNP:200580141
1208 1208 c, g dbSNP:771195007
1212 1212 c, g dbSNP:776787195
1222 1222 a, g dbSNP:145547756
1239 1239 c, t dbSNP:749879794
1240 1240 a, g dbSNP:755690043
1242 1242 a, g dbSNP:765946876
1245 1245 a, g dbSNP:753606554
1264 1264 c, t dbSNP:34714325
1267 1267 c, t dbSNP:372640050
1268 1268 a, g, t dbSNP:748864570
1269 1269 c, t dbSNP:372042331
1270 1270 a, g dbSNP:745927157
1291 1291 a, t dbSNP:769974312
1304 1304 c, t dbSNP:770681879
1305 1305 a, g dbSNP:749444399
1308 1308 c, t dbSNP:768895564
1309 1309 a, g dbSNP:774085700
1313 1313 c, t dbSNP:569471270
1314 1314 a, g dbSNP:138134300
1318 1318 c, t dbSNP:140957456
1319 1319 a, c, g dbSNP:774521447
1329 1329 c, t dbSNP:34184265
1342 1342 a, g dbSNP:753406137
1343 1343 c, t dbSNP:759213296
1344 1344 a, g dbSNP:376217797
1354 1354 a, c dbSNP:751938866
1356 1356 c, t dbSNP:757703975
1357 1357 a, g dbSNP:199705255
1359 1359 c, t dbSNP:750883093
1361 1361 c, t dbSNP:35117690
1362 1362 a, c, g dbSNP:534357183
1365 1365 a, c, t dbSNP:148105023
1366 1366 a, g dbSNP:371075114
1379 1379 g, t dbSNP:762624356
1382 1382 -, cta dbSNP:757624406
1384 1384 g, t dbSNP:763673900
1386 1386 c, t dbSNP:376330766
1387 1387 a, g dbSNP:760987653
1405 1405 a, t dbSNP:766867142
1406 1406 a, g dbSNP:773253990
1417 1417 a, t dbSNP:368231126
1418 1418 a, g, t dbSNP:765237852
1421 1421 c, t dbSNP:141885543
1422 1422 a, g dbSNP:372499415
1426 1426 c, t dbSNP:746812707
1427 1427 a, g dbSNP:757173532
1437 1437 c, t dbSNP:562103199
1444 1444 c, t dbSNP:745733849
1445 1445 a, g dbSNP:769882100
1451 1451 a, g dbSNP:775015647
1452 1452 c, g dbSNP:369242634
1461 1461 c, t dbSNP:574652943
1462 1462 a, g dbSNP:768325811
1464 1464 g, t dbSNP:773944166
1479 1479 c, t dbSNP:762077860
1482 1482 c, g dbSNP:150649154
1489 1489 c, t dbSNP:750211278
1494 1494 a, g dbSNP:756041065
1497 1497 a, g dbSNP:780029811
1498 1498 c, g dbSNP:753660268
1506 1506 a, c, t dbSNP:369277668
1519 1519 c, t dbSNP:759672045
1520 1520 a, g dbSNP:747683414
1526 1526 a, g dbSNP:771692459
1533 1533 a, g dbSNP:2230272
1551 1551 a, g dbSNP:746214599
1554 1554 c, t dbSNP:139855095
1555 1555 a, g dbSNP:775924004
1560 1560 a, g dbSNP:763329061
1562 1562 g, t dbSNP:769227468
1570 1570 c, t dbSNP:774548797
1586 1586 a, t dbSNP:202182009
1591 1591 a, g dbSNP:201735991
1606 1606 a, c dbSNP:756837478
1611 1611 c, t dbSNP:780936222
1614 1614 a, g dbSNP:370388643
1622 1622 a, c dbSNP:755429809
1630 1630 a, g dbSNP:779435896
1632 1632 c, t dbSNP:199594802
1633 1633 a, g dbSNP:143044080
1636 1636 c, t dbSNP:202060851
1646 1646 c, t dbSNP:747166742
1647 1647 a, g, t dbSNP:771030415
1653 1653 c, t dbSNP:749869691
1654 1654 a, g dbSNP:769508911
1666 1666 c, t dbSNP:761670678
1667 1667 a, g dbSNP:766986473
1671 1671 a, g dbSNP:368503704
1677 1677 c, t dbSNP:35050271
1684 1684 c, g dbSNP:760356335
1689 1689 a, c, t dbSNP:140195872
1698 1698 a, c, t dbSNP:780959057
1699 1699 a, g dbSNP:145516111
1701 1701 c, t dbSNP:550940952
1703 1703 g, t dbSNP:778133808
1707 1707 -, a dbSNP:768753859
1710 1710 c, t dbSNP:752034138
1711 1711 c, t dbSNP:144329353
1712 1712 a, g dbSNP:781282147
1716 1716 c, g dbSNP:148777860
1719 1719 a, g dbSNP:745993283
1728 1728 c, t dbSNP:145555537
1734 1734 a, g dbSNP:763945442
1736 1736 g, t dbSNP:35413228
1741 1741 c, t dbSNP:757123518
1742 1742 a, g dbSNP:368989673
1752 1752 c, t dbSNP:749898309
1754 1754 a, t dbSNP:755584243
1758 1758 c, t dbSNP:61731875
1761 1761 c, t dbSNP:748889875
1762 1762 a, g dbSNP:754656515
1765 1765 a, t dbSNP:778078199
1769 1769 a, g dbSNP:747448805
1770 1770 c, t dbSNP:771325150
1772 1772 a, g dbSNP:781754660
1778 1778 g, t dbSNP:765714125
1780 1780 c, g, t dbSNP:373386969
1781 1781 a, g dbSNP:188624660
1785 1785 c, g, t dbSNP:374134958
1786 1786 a, g dbSNP:193259523
1792 1792 a, c, g dbSNP:761737367
1809 1809 c, g dbSNP:762414101
1813 1813 c, t dbSNP:750318120
1815 1815 c, t dbSNP:760551092
1817 1817 a, g dbSNP:201758210
1820 1820 c, t dbSNP:765835251
1821 1821 c, g dbSNP:753384342
1822 1822 c, t dbSNP:754601463
1824 1824 c, t dbSNP:753086468
1834 1834 c, t dbSNP:553362925
1835 1835 a, g dbSNP:3750665
1858 1858 c, g dbSNP:542881129
1865 1865 c, t dbSNP:561001045
1868 1868 c, t dbSNP:751150752
1869 1869 a, g dbSNP:114458714
1895 1895 a, g dbSNP:543203585
1897 1897 c, g dbSNP:564684755
1905 1905 c, t dbSNP:532028772
1906 1906 a, g dbSNP:577319547
1915 1915 c, t dbSNP:546996786
1923 1923 g, t dbSNP:11257460
1936 1936 c, t dbSNP:752489515
1937 1937 c, t dbSNP:760685079
1941 1941 a, c, t dbSNP:370060304
1942 1942 g, t dbSNP:756054427
1966 1966 a, g dbSNP:548899943
1978 1978 c, t dbSNP:373062080
1979 1979 a, g, t dbSNP:111841872
1993 1993 c, t dbSNP:539072183
2003 2003 c, t dbSNP:41291285
2006 2006 c, t dbSNP:570967445
2008 2008 c, t dbSNP:535086675
2013 2013 c, t dbSNP:1539232
2048 2048 c, g, t dbSNP:56656269
2056 2056 a, g dbSNP:555154575
2072 2072 a, g dbSNP:747250693
2076 2076 a, g dbSNP:188911010
2091 2091 a, g dbSNP:1539233
2104 2104 g, t dbSNP:554887905
2175 2175 c, t dbSNP:576254554
2201 2201 c, g dbSNP:766884294
2240 2240 -, c dbSNP:34059583
2257 2257 c, t dbSNP:543213777
2288 2288 c, t dbSNP:564694905
2289 2289 a, g dbSNP:543925758
2314 2314 c, g dbSNP:41291287
2336 2336 c, g dbSNP:149180628
2344 2344 -, c dbSNP:542213461
2345 2345 c, t dbSNP:529736533
2366 2366 a, g dbSNP:11541590
2367 2367 c, t dbSNP:763468630
2395 2395 c, t dbSNP:770357758
2401 2401 a, t dbSNP:115020525
2458 2458 c, g dbSNP:531189229
2470 2470 c, t dbSNP:3750667
2490 2490 a, c, t dbSNP:571030665
2497 2497 g, t dbSNP:759211530
2520 2520 a, c dbSNP:767121904
2522 2522 c, g dbSNP:535210931
2541 2541 c, g dbSNP:75380779
2552 2552 g, t dbSNP:568451349
2564 2564 g, t dbSNP:371109958
2584 2584 c, g dbSNP:752504378
2614 2614 c, g dbSNP:760346345
2617 2617 a, c dbSNP:138719592
2635 2635 c, t dbSNP:576292121
2640 2640 a, g dbSNP:36102318
2644 2644 c, t dbSNP:55643411
2652 2652 c, g dbSNP:3829148
2664 2664 a, g dbSNP:540819800
2665 2665 g, t dbSNP:751642477
2690 2690 c, t dbSNP:181624552
2691 2691 a, g dbSNP:574405068
2727 2727 c, g, t dbSNP:1064887
2766 2766 a, g dbSNP:563104441
2772 2772 c, t dbSNP:116150030
2775 2775 a, t dbSNP:1064888
2776 2776 c, t dbSNP:552934020
2790 2790 c, g dbSNP:564752658
2798 2798 a, g dbSNP:755198570
2802 2802 a, g dbSNP:528734720
2808 2808 c, t dbSNP:546873537
2899 2899 a, g dbSNP:568541518
2903 2903 a, g dbSNP:35781052
2914 2914 a, g dbSNP:146927431
2938 2938 a, g dbSNP:550968611
2977 2977 c, t dbSNP:137871718
2993 2993 c, t dbSNP:183868086
2994 2994 g, t dbSNP:188597321
3032 3032 a, g dbSNP:778111157
3041 3041 a, g dbSNP:576724255
3047 3047 c, g dbSNP:534820299
3097 3097 c, t dbSNP:749787572
3105 3105 g, t dbSNP:552851905
3114 3114 c, t dbSNP:574493771
3124 3124 g, t dbSNP:541534421
3142 3142 c, t dbSNP:563377725
3171 3171 -, att dbSNP:767685301
3209 3209 a, g dbSNP:771483643
3250 3250 a, g dbSNP:1064889
3288 3288 a, g dbSNP:745655832
3347 3347 c, t dbSNP:575348110
3353 3353 c, g dbSNP:546087445
3354 3354 a, g dbSNP:545803813
3357 3357 c, g dbSNP:1064890
3362 3362 c, t dbSNP:373060819
3377 3377 c, t dbSNP:564814115
3378 3378 c, t dbSNP:11541589
3386 3386 a, c dbSNP:561156068
3400 3400 c, t dbSNP:773887335
3401 3401 a, g dbSNP:771717149
3406 3406 c, t dbSNP:528746797
3409 3409 a, g dbSNP:759007137
3418 3418 c, t dbSNP:771642038
3424 3424 c, t dbSNP:540827250
3425 3425 a, g dbSNP:180716582
3426 3426 c, t dbSNP:531498608
3427 3427 a, g dbSNP:113076566
3428 3428 c, t dbSNP:550822376
3430 3430 c, g dbSNP:150577187
3434 3434 -, tc dbSNP:201934407
3442 3442 c, t dbSNP:1064891
3452 3452 c, t dbSNP:574236062
3460 3460 c, t dbSNP:571800119
3477 3477 a, g dbSNP:1064892
3479 3479 g, t dbSNP:551315848
3500 3500 a, g, t dbSNP:111928593
3506 3506 a, g dbSNP:149295239
3562 3562 a, t dbSNP:1064893
3587 3587 c, t dbSNP:186096730
3588 3588 a, g, t dbSNP:3814190
3591 3591 c, g dbSNP:1064894
3608 3608 a, c dbSNP:1064895
3611 3611 a, g dbSNP:1539234
3619 3619 a, t dbSNP:1064896
3633 3633 a, g dbSNP:1064897
3660 3660 c, t dbSNP:547813518
3705 3705 c, t dbSNP:190957966
3712 3712 c, t dbSNP:765521774
3729 3729 a, g dbSNP:373840460
3741 3741 c, t dbSNP:545815893
3752 3752 a, g dbSNP:557742706
3799 3799 c, g dbSNP:573594459
3803 3803 a, c dbSNP:1064898
3823 3823 c, t dbSNP:562383215
3841 3841 c, t dbSNP:565896863
3842 3842 -, ggacggg dbSNP:575355598
3852 3852 a, c dbSNP:113438580
3853 3853 c, t dbSNP:746211569
3867 3867 c, g dbSNP:1064899
3878 3878 -, ac dbSNP:759507402
3880 3880 a, c dbSNP:1064900
3935 3935 c, t dbSNP:773353443
3936 3936 a, g dbSNP:752799904
3941 3941 c, g dbSNP:756391076
3954 3954 c, t dbSNP:529496552
3979 3979 a, t dbSNP:763256173
3982 3982 -, c dbSNP:545922011
3987 3987 -, cc dbSNP:57936464
3989 3989 c, t dbSNP:146257115
4028 4028 c, t dbSNP:562645839
4030 4030 g, t dbSNP:182354027
4082 4082 c, t dbSNP:778092840
4084 4084 c, g dbSNP:551377173
4093 4093 c, t dbSNP:566503745
4111 4111 a, g dbSNP:766589456
4121 4121 g, t dbSNP:186547454
4147 4147 -, g dbSNP:564323726
4222 4222 -, a dbSNP:147522216
4224 4224 c, t dbSNP:372346664
4248 4248 g, t dbSNP:371173465
4259 4259 a, c dbSNP:74795188
4261 4261 a, g dbSNP:536956181
4325 4325 a, t dbSNP:568337048
4350 4350 c, g dbSNP:76767288

Target ORF information:

RefSeq Version XM_005252464
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005252464

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGGG CGTCTGTGAG GAGCTGACCT ACGAGGAGAT CAGGGACACC
TACCCTGAGG AGTATGCGCT GCGGGAGCAG GACAAGTACT ATTACCGCTA CCCCACCGGG
GAGTCCTACC AGGACCTGGT CCAGCGCTTG GAGCCAGTGA TCATGGAGCT GGAGCGGCAG
GAGAATGTGC TGGTCATCTG CCACCAGGCC GTCCTGCGCT GCCTGCTTGC CTACTTCCTG
GATAAGAGTG CAGAGGAGAT GCCCTACCTG AAATGCCCTC TTCACACCGT CCTGAAACTG
ACGCCTGTCG CTTATGGCTG CCGTGTGGAA TCCATCTACC TGAACGTGGA GTCCGTCTGC
ACACACCGGG AGAGGTCAGA GAACATGAAA GGCTCCCGGA GCAGCGCTGA CTCCTCCAGG
AAACACTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu59629D
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008705.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGTC CTACCAGGAC CTGGTCCAGC GCTTGGAGCC AGTGATCATG
GAGCTGGAGC GGCAGGAGAA TGTGCTGGTC ATCTGCCACC AGGCCGTCCT GCGCTGCCTG
CTTGCCTACT TCCTGGATAA GAGTGCAGAG GAGATGCCCT ACCTGAAATG CCCTCTTCAC
ACCGTCCTGA AACTGACGCC TGTCGCTTAT GGCTGCCGTG TGGAATCCAT CTACCTGAAC
GTGGAGTCCG TCTGCACACA CCGGGAGAGG TCAGAGAACA TGAAAGGCTC CCGGAGCAGC
GCTGACTCCT CCAGGAAACA CTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011517795.1
CDS385..1668
Misc Feature(1)460..1122(+)
Misc Feature(2)1126..1581(+)
Misc Feature(3)1141..1446(+)
Translation
Position Chain Variation Link
19 19 c, g dbSNP:11257234
35 35 c, t dbSNP:553359827
37 37 a, g dbSNP:11257235
46 46 a, g dbSNP:74379289
65 65 a, g dbSNP:549825839
78 78 a, g dbSNP:560979015
80 80 a, g dbSNP:617245
86 86 a, g dbSNP:543607586
151 151 c, t dbSNP:565058212
163 163 c, g dbSNP:532277271
195 195 a, g dbSNP:547174306
200 200 -, g dbSNP:527630990
203 203 c, t dbSNP:760720377
206 206 c, g dbSNP:557166161
237 237 c, t dbSNP:149331745
245 245 c, t dbSNP:773328449
256 256 c, g dbSNP:764350812
276 276 c, g dbSNP:11596368
291 291 c, g dbSNP:548865023
292 292 -, ct dbSNP:368508609
299 299 c, t dbSNP:570222395
317 317 c, g, t dbSNP:2387004
335 335 a, g dbSNP:202167699
339 339 c, t dbSNP:558760497
342 342 a, g dbSNP:762968358
347 347 c, t dbSNP:369169953
348 348 c, g, t dbSNP:570689483
350 350 c, t dbSNP:767355262
351 351 c, t dbSNP:750229279
361 361 g, t dbSNP:755976157
364 364 c, g dbSNP:376534878
365 365 a, c dbSNP:753338368
368 368 c, t dbSNP:754495467
369 369 -, gg dbSNP:4002912
369 369 a, g dbSNP:553181137
374 374 c, t dbSNP:747812819
379 379 c, g dbSNP:771768885
384 384 c, g dbSNP:370673747
390 390 c, g dbSNP:746264347
397 397 c, g dbSNP:770373341
408 408 c, t dbSNP:368385191
432 432 c, t dbSNP:201238659
433 433 g, t dbSNP:768690057
436 436 a, g dbSNP:774623394
441 441 c, g, t dbSNP:761887123
450 450 c, g dbSNP:750180429
453 453 c, g dbSNP:760486257
458 458 a, c, g dbSNP:200996135
460 460 a, t dbSNP:751708658
461 461 c, t dbSNP:758028195
467 467 a, g dbSNP:763805870
486 486 a, c dbSNP:567913373
487 487 c, t dbSNP:756538480
489 489 a, c dbSNP:528993171
492 492 c, t dbSNP:371698685
493 493 a, g dbSNP:200349885
498 498 c, t dbSNP:779499668
499 499 a, g dbSNP:142603218
516 516 c, t dbSNP:146319524
517 517 a, g dbSNP:759203021
520 520 c, t dbSNP:747176453
546 546 -, gctgactc dbSNP:747356467
552 552 g, t dbSNP:138221143
558 558 c, t dbSNP:776247912
559 559 c, t dbSNP:567196228
564 564 c, t dbSNP:765060646
572 572 a, g dbSNP:775535420
574 574 a, g dbSNP:375018306
576 576 c, t dbSNP:763750903
587 587 c, t dbSNP:781263393
594 594 c, t dbSNP:746156494
595 595 a, g dbSNP:181651478
597 597 -, g dbSNP:780889322
597 597 c, t dbSNP:374201933
610 610 c, t dbSNP:749129357
626 626 a, g dbSNP:547569491
629 629 a, g dbSNP:774481512
631 631 a, g dbSNP:376208847
649 649 c, t dbSNP:771681077
651 651 c, t dbSNP:772727967
655 655 a, g dbSNP:760318737
657 657 c, t dbSNP:199980015
658 658 a, c dbSNP:753009870
664 664 a, g dbSNP:149555675
671 671 c, t dbSNP:764552151
679 679 c, t dbSNP:201889586
680 680 a, g dbSNP:36005730
683 683 a, g dbSNP:757268684
693 693 c, t dbSNP:542439439
695 695 c, t dbSNP:773635607
698 698 c, g dbSNP:760834564
700 700 g, t dbSNP:766625430
715 715 a, c dbSNP:753980509
716 716 a, g dbSNP:765641319
728 728 a, c, t dbSNP:765399775
729 729 a, g dbSNP:750805171
733 733 c, g, t dbSNP:763367903
750 750 a, g dbSNP:757197649
756 756 c, t dbSNP:748952044
770 770 c, t dbSNP:768370594
790 790 a, g dbSNP:777933166
809 809 a, g dbSNP:747409261
822 822 c, t dbSNP:138054261
827 827 c, t dbSNP:144243977
828 828 a, g dbSNP:34969226
837 837 c, t dbSNP:754555836
838 838 a, g dbSNP:773437490
841 841 c, t dbSNP:576701454
842 842 c, t dbSNP:764975677
843 843 a, g dbSNP:79668213
844 844 a, g dbSNP:758136512
845 845 c, t dbSNP:758712431
849 849 c, t dbSNP:746271169
850 850 a, g dbSNP:756527360
852 852 c, t dbSNP:573415324
853 853 a, g dbSNP:749299504
870 870 c, g dbSNP:374769986
877 877 a, g dbSNP:776918283
881 881 c, t dbSNP:774547245
888 888 a, g, t dbSNP:201048797
892 892 a, g dbSNP:373530404
896 896 a, c dbSNP:769828653
913 913 a, g dbSNP:775606147
920 920 a, t dbSNP:762545765
923 923 c, t dbSNP:768274515
924 924 a, g dbSNP:377330736
934 934 a, g dbSNP:761465902
938 938 a, g dbSNP:767107653
939 939 c, t dbSNP:545085751
956 956 a, t dbSNP:759944528
963 963 c, t dbSNP:147548407
969 969 a, c dbSNP:753259168
970 970 a, g dbSNP:758918175
982 982 a, c dbSNP:764167016
987 987 c, t dbSNP:751620453
988 988 a, g dbSNP:757448210
993 993 -, gacaaat dbSNP:765391948
993 993 c, t dbSNP:140074833
994 994 a, g dbSNP:199606482
999 999 a, g dbSNP:756036883
1002 1002 c, g, t dbSNP:780129830
1009 1009 a, g dbSNP:771551601
1016 1016 c, t dbSNP:772721261
1017 1017 a, g, t dbSNP:145567510
1020 1020 a, g dbSNP:558126063
1024 1024 a, c dbSNP:763272837
1031 1031 c, t dbSNP:369033520
1035 1035 c, t dbSNP:150949806
1036 1036 a, g dbSNP:373816623
1042 1042 c, t dbSNP:767650284
1043 1043 a, g dbSNP:140784797
1054 1054 c, g dbSNP:577158958
1061 1061 a, g dbSNP:766735374
1084 1084 c, t dbSNP:753664642
1085 1085 a, g dbSNP:765237719
1086 1086 c, t dbSNP:541321493
1087 1087 a, g dbSNP:752636577
1089 1089 c, t dbSNP:2230271
1090 1090 a, g dbSNP:777223374
1094 1094 a, g dbSNP:746543536
1113 1113 c, t dbSNP:376533039
1114 1114 a, g dbSNP:369124719
1120 1120 c, g dbSNP:749656403
1121 1121 c, t dbSNP:373844823
1122 1122 a, g dbSNP:774789106
1124 1124 a, g dbSNP:762163058
1146 1146 c, t dbSNP:772740743
1147 1147 a, g dbSNP:773379850
1155 1155 c, g, t dbSNP:145448801
1156 1156 a, g dbSNP:574908611
1158 1158 c, g dbSNP:759364786
1160 1160 a, g dbSNP:764872013
1161 1161 c, g dbSNP:752567472
1165 1165 c, g dbSNP:758281350
1174 1174 c, t dbSNP:140258391
1175 1175 a, g, t dbSNP:377041246
1179 1179 a, c, t dbSNP:370128069
1180 1180 a, g dbSNP:34832528
1182 1182 g, t dbSNP:745451556
1185 1185 c, t dbSNP:769493203
1192 1192 a, g dbSNP:12569742
1196 1196 c, t dbSNP:779109196
1202 1202 a, g dbSNP:748570830
1204 1204 a, c, t dbSNP:200723320
1205 1205 a, g dbSNP:200580141
1208 1208 c, g dbSNP:771195007
1212 1212 c, g dbSNP:776787195
1222 1222 a, g dbSNP:145547756
1239 1239 c, t dbSNP:749879794
1240 1240 a, g dbSNP:755690043
1242 1242 a, g dbSNP:765946876
1245 1245 a, g dbSNP:753606554
1264 1264 c, t dbSNP:34714325
1267 1267 c, t dbSNP:372640050
1268 1268 a, g, t dbSNP:748864570
1269 1269 c, t dbSNP:372042331
1270 1270 a, g dbSNP:745927157
1291 1291 a, t dbSNP:769974312
1304 1304 c, t dbSNP:770681879
1305 1305 a, g dbSNP:749444399
1308 1308 c, t dbSNP:768895564
1309 1309 a, g dbSNP:774085700
1313 1313 c, t dbSNP:569471270
1314 1314 a, g dbSNP:138134300
1318 1318 c, t dbSNP:140957456
1319 1319 a, c, g dbSNP:774521447
1329 1329 c, t dbSNP:34184265
1342 1342 a, g dbSNP:753406137
1343 1343 c, t dbSNP:759213296
1344 1344 a, g dbSNP:376217797
1354 1354 a, c dbSNP:751938866
1356 1356 c, t dbSNP:757703975
1357 1357 a, g dbSNP:199705255
1359 1359 c, t dbSNP:750883093
1361 1361 c, t dbSNP:35117690
1362 1362 a, c, g dbSNP:534357183
1374 1374 c, t dbSNP:762077860
1377 1377 c, g dbSNP:150649154
1384 1384 c, t dbSNP:750211278
1389 1389 a, g dbSNP:756041065
1392 1392 a, g dbSNP:780029811
1393 1393 c, g dbSNP:753660268
1401 1401 a, c, t dbSNP:369277668
1414 1414 c, t dbSNP:759672045
1415 1415 a, g dbSNP:747683414
1421 1421 a, g dbSNP:771692459
1428 1428 a, g dbSNP:2230272
1446 1446 a, g dbSNP:746214599
1449 1449 c, t dbSNP:139855095
1450 1450 a, g dbSNP:775924004
1455 1455 a, g dbSNP:763329061
1457 1457 g, t dbSNP:769227468
1465 1465 c, t dbSNP:774548797
1481 1481 a, t dbSNP:202182009
1486 1486 a, g dbSNP:201735991
1501 1501 a, c dbSNP:756837478
1506 1506 c, t dbSNP:780936222
1509 1509 a, g dbSNP:370388643
1517 1517 a, c dbSNP:755429809
1525 1525 a, g dbSNP:779435896
1527 1527 c, t dbSNP:199594802
1528 1528 a, g dbSNP:143044080
1531 1531 c, t dbSNP:202060851
1541 1541 c, t dbSNP:747166742
1542 1542 a, g, t dbSNP:771030415
1548 1548 c, t dbSNP:749869691
1549 1549 a, g dbSNP:769508911
1561 1561 c, t dbSNP:761670678
1562 1562 a, g dbSNP:766986473
1566 1566 a, g dbSNP:368503704
1572 1572 c, t dbSNP:35050271
1579 1579 c, g dbSNP:760356335
1584 1584 a, c, t dbSNP:140195872
1593 1593 a, c, t dbSNP:780959057
1594 1594 a, g dbSNP:145516111
1596 1596 c, t dbSNP:550940952
1598 1598 g, t dbSNP:778133808
1602 1602 -, a dbSNP:768753859
1605 1605 c, t dbSNP:752034138
1606 1606 c, t dbSNP:144329353
1607 1607 a, g dbSNP:781282147
1611 1611 c, g dbSNP:148777860
1614 1614 a, g dbSNP:745993283
1623 1623 c, t dbSNP:145555537
1629 1629 a, g dbSNP:763945442
1631 1631 g, t dbSNP:35413228
1636 1636 c, t dbSNP:757123518
1637 1637 a, g dbSNP:368989673
1647 1647 c, t dbSNP:749898309
1649 1649 a, t dbSNP:755584243
1653 1653 c, t dbSNP:61731875
1656 1656 c, t dbSNP:748889875
1657 1657 a, g dbSNP:754656515
1660 1660 a, t dbSNP:778078199
1664 1664 a, g dbSNP:747448805
1665 1665 c, t dbSNP:771325150
1667 1667 a, g dbSNP:781754660
1673 1673 g, t dbSNP:765714125
1675 1675 c, g, t dbSNP:373386969
1676 1676 a, g dbSNP:188624660
1680 1680 c, g, t dbSNP:374134958
1681 1681 a, g dbSNP:193259523
1687 1687 a, c, g dbSNP:761737367
1704 1704 c, g dbSNP:762414101
1708 1708 c, t dbSNP:750318120
1710 1710 c, t dbSNP:760551092
1712 1712 a, g dbSNP:201758210
1715 1715 c, t dbSNP:765835251
1716 1716 c, g dbSNP:753384342
1717 1717 c, t dbSNP:754601463
1719 1719 c, t dbSNP:753086468
1729 1729 c, t dbSNP:553362925
1730 1730 a, g dbSNP:3750665
1753 1753 c, g dbSNP:542881129
1760 1760 c, t dbSNP:561001045
1763 1763 c, t dbSNP:751150752
1764 1764 a, g dbSNP:114458714
1790 1790 a, g dbSNP:543203585
1792 1792 c, g dbSNP:564684755
1800 1800 c, t dbSNP:532028772
1801 1801 a, g dbSNP:577319547
1810 1810 c, t dbSNP:546996786
1818 1818 g, t dbSNP:11257460
1831 1831 c, t dbSNP:752489515
1832 1832 c, t dbSNP:760685079
1836 1836 a, c, t dbSNP:370060304
1837 1837 g, t dbSNP:756054427
1861 1861 a, g dbSNP:548899943
1873 1873 c, t dbSNP:373062080
1874 1874 a, g, t dbSNP:111841872
1888 1888 c, t dbSNP:539072183
1898 1898 c, t dbSNP:41291285
1901 1901 c, t dbSNP:570967445
1903 1903 c, t dbSNP:535086675
1908 1908 c, t dbSNP:1539232
1943 1943 c, g, t dbSNP:56656269
1951 1951 a, g dbSNP:555154575
1967 1967 a, g dbSNP:747250693
1971 1971 a, g dbSNP:188911010
1986 1986 a, g dbSNP:1539233
1999 1999 g, t dbSNP:554887905
2070 2070 c, t dbSNP:576254554
2096 2096 c, g dbSNP:766884294
2135 2135 -, c dbSNP:34059583
2152 2152 c, t dbSNP:543213777
2183 2183 c, t dbSNP:564694905
2184 2184 a, g dbSNP:543925758
2209 2209 c, g dbSNP:41291287
2231 2231 c, g dbSNP:149180628
2239 2239 -, c dbSNP:542213461
2240 2240 c, t dbSNP:529736533
2261 2261 a, g dbSNP:11541590
2262 2262 c, t dbSNP:763468630
2290 2290 c, t dbSNP:770357758
2296 2296 a, t dbSNP:115020525
2353 2353 c, g dbSNP:531189229
2365 2365 c, t dbSNP:3750667
2385 2385 a, c, t dbSNP:571030665
2392 2392 g, t dbSNP:759211530
2415 2415 a, c dbSNP:767121904
2417 2417 c, g dbSNP:535210931
2436 2436 c, g dbSNP:75380779
2447 2447 g, t dbSNP:568451349
2459 2459 g, t dbSNP:371109958
2479 2479 c, g dbSNP:752504378
2509 2509 c, g dbSNP:760346345
2512 2512 a, c dbSNP:138719592
2530 2530 c, t dbSNP:576292121
2535 2535 a, g dbSNP:36102318
2539 2539 c, t dbSNP:55643411
2547 2547 c, g dbSNP:3829148
2559 2559 a, g dbSNP:540819800
2560 2560 g, t dbSNP:751642477
2585 2585 c, t dbSNP:181624552
2586 2586 a, g dbSNP:574405068
2622 2622 c, g, t dbSNP:1064887
2661 2661 a, g dbSNP:563104441
2667 2667 c, t dbSNP:116150030
2670 2670 a, t dbSNP:1064888
2671 2671 c, t dbSNP:552934020
2685 2685 c, g dbSNP:564752658
2693 2693 a, g dbSNP:755198570
2697 2697 a, g dbSNP:528734720
2703 2703 c, t dbSNP:546873537
2794 2794 a, g dbSNP:568541518
2798 2798 a, g dbSNP:35781052
2809 2809 a, g dbSNP:146927431
2833 2833 a, g dbSNP:550968611
2872 2872 c, t dbSNP:137871718
2888 2888 c, t dbSNP:183868086
2889 2889 g, t dbSNP:188597321
2927 2927 a, g dbSNP:778111157
2936 2936 a, g dbSNP:576724255
2942 2942 c, g dbSNP:534820299
2992 2992 c, t dbSNP:749787572
3000 3000 g, t dbSNP:552851905
3009 3009 c, t dbSNP:574493771
3019 3019 g, t dbSNP:541534421
3037 3037 c, t dbSNP:563377725
3066 3066 -, att dbSNP:767685301
3104 3104 a, g dbSNP:771483643
3145 3145 a, g dbSNP:1064889
3183 3183 a, g dbSNP:745655832
3242 3242 c, t dbSNP:575348110
3248 3248 c, g dbSNP:546087445
3249 3249 a, g dbSNP:545803813
3252 3252 c, g dbSNP:1064890
3257 3257 c, t dbSNP:373060819
3272 3272 c, t dbSNP:564814115
3273 3273 c, t dbSNP:11541589
3281 3281 a, c dbSNP:561156068
3295 3295 c, t dbSNP:773887335
3296 3296 a, g dbSNP:771717149
3301 3301 c, t dbSNP:528746797
3304 3304 a, g dbSNP:759007137
3313 3313 c, t dbSNP:771642038
3319 3319 c, t dbSNP:540827250
3320 3320 a, g dbSNP:180716582
3321 3321 c, t dbSNP:531498608
3322 3322 a, g dbSNP:113076566
3323 3323 c, t dbSNP:550822376
3325 3325 c, g dbSNP:150577187
3329 3329 -, tc dbSNP:201934407
3337 3337 c, t dbSNP:1064891
3347 3347 c, t dbSNP:574236062
3355 3355 c, t dbSNP:571800119
3372 3372 a, g dbSNP:1064892
3374 3374 g, t dbSNP:551315848
3395 3395 a, g, t dbSNP:111928593
3401 3401 a, g dbSNP:149295239
3457 3457 a, t dbSNP:1064893
3482 3482 c, t dbSNP:186096730
3483 3483 a, g, t dbSNP:3814190
3486 3486 c, g dbSNP:1064894
3503 3503 a, c dbSNP:1064895
3506 3506 a, g dbSNP:1539234
3514 3514 a, t dbSNP:1064896
3528 3528 a, g dbSNP:1064897
3555 3555 c, t dbSNP:547813518
3600 3600 c, t dbSNP:190957966
3607 3607 c, t dbSNP:765521774
3624 3624 a, g dbSNP:373840460
3636 3636 c, t dbSNP:545815893
3647 3647 a, g dbSNP:557742706
3694 3694 c, g dbSNP:573594459
3698 3698 a, c dbSNP:1064898
3718 3718 c, t dbSNP:562383215
3736 3736 c, t dbSNP:565896863
3737 3737 -, ggacggg dbSNP:575355598
3747 3747 a, c dbSNP:113438580
3748 3748 c, t dbSNP:746211569
3762 3762 c, g dbSNP:1064899
3773 3773 -, ac dbSNP:759507402
3775 3775 a, c dbSNP:1064900
3830 3830 c, t dbSNP:773353443
3831 3831 a, g dbSNP:752799904
3836 3836 c, g dbSNP:756391076
3849 3849 c, t dbSNP:529496552
3874 3874 a, t dbSNP:763256173
3877 3877 -, c dbSNP:545922011
3882 3882 -, cc dbSNP:57936464
3884 3884 c, t dbSNP:146257115
3923 3923 c, t dbSNP:562645839
3925 3925 g, t dbSNP:182354027
3977 3977 c, t dbSNP:778092840
3979 3979 c, g dbSNP:551377173
3988 3988 c, t dbSNP:566503745
4006 4006 a, g dbSNP:766589456
4016 4016 g, t dbSNP:186547454
4042 4042 -, g dbSNP:564323726
4117 4117 -, a dbSNP:147522216
4119 4119 c, t dbSNP:372346664
4143 4143 g, t dbSNP:371173465
4154 4154 a, c dbSNP:74795188
4156 4156 a, g dbSNP:536956181
4220 4220 a, t dbSNP:568337048
4245 4245 c, g dbSNP:76767288

Target ORF information:

RefSeq Version XM_011519493
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011519493

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGTC CTACCAGGAC CTGGTCCAGC GCTTGGAGCC AGTGATCATG
GAGCTGGAGC GGCAGGAGAA TGTGCTGGTC ATCTGCCACC AGGCCGTCCT GCGCTGCCTG
CTTGCCTACT TCCTGGATAA GAGTGCAGAG GAGATGCCCT ACCTGAAATG CCCTCTTCAC
ACCGTCCTGA AACTGACGCC TGTCGCTTAT GGCTGCCGTG TGGAATCCAT CTACCTGAAC
GTGGAGTCCG TCTGCACACA CCGGGAGAGG TCAGAGAACA TGAAAGGCTC CCGGAGCAGC
GCTGACTCCT CCAGGAAACA CTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu14576D
Sequence Information ORF Nucleotide Sequence (Length: 1605bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 3
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK304450.1, DC316967.1, L77662.1, AL359960.23 and AI633567.1. On Sep 17, 2013 this sequence version replaced gi:530392180. Transcript Variant: This variant (3) uses an alternate 5' terminal exon compared to variant 1. The resulting isoform (3) has a longer and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304450.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGGGGAGG GTGGCCAGAA GGAAGGTGAC AGCCAGCAAG CAGGGGCTCT GCCACTCCTC 
TGTCAGCTGG ACACGTTTAG TCCCAAGGCC ACTGTCTTCG GTGTCTCCAT TAATCCAGCC
TGTGGGCCAA AGCTGACCAA CTCCCCCACC GTCATCGTCA TGGTGGGCCT CCCCGCCCGG
GGCAAGACCT ACATCTCCAA GAAGCTGACT CGCTACCTCA ACTGGATTGG CGTCCCCACA
AAAGTGTTCA ACGTCGGGGA GTATCGCCGG GAGGCTGTGA AGCAGTACAG CTCCTACAAC
TTCTTCCGCC CCGACAATGA GGAAGCCATG AAAGTCCGGA AGCAATGTGC CTTAGCTGCC
TTGAGAGATG TCAAAAGCTA CCTGGCGAAA GAAGGGGGAC AAATTGCGGT TTTCGATGCC
ACCAATACTA CTAGAGAGAG GAGACACATG ATCCTTCATT TTGCCAAAGA AAATGACTTT
AAGGCGTTTT TCATCGAGTC GGTGTGCGAC GACCCTACAG TTGTGGCCTC CAATATCATG
GAAGTTAAAA TCTCCAGCCC GGATTACAAA GACTGCAACT CGGCAGAAGC CATGGACGAC
TTCATGAAGA GGATCAGTTG CTATGAAGCC AGCTACCAGC CCCTCGACCC CGACAAATGC
GACAGGGACT TGTCGCTGAT CAAGGTGATT GACGTGGGCC GGAGGTTCCT GGTGAACCGG
GTGCAGGACC ACATCCAGAG CCGCATCGTG TACTACCTGA TGAACATCCA CGTGCAGCCG
CGTACCATCT ACCTGTGCCG GCACGGCGAG AACGAGCACA ACCTCCAGGG CCGCATCGGG
GGCGACTCAG GCCTGTCCAG CCGGGGCAAG AAGTTTGCCA GTGCTCTGAG CAAGTTCGTG
GAGGAGCAGA ACCTGAAGGA CCTGCGCGTG TGGACCAGCC AGCTGAAGAG CACCATCCAG
ACGGCCGAGG CGCTGCGGCT GCCCTACGAG CAGTGGAAGG CGCTCAATGA GATCGACGCG
GGCGTCTGTG AGGAGCTGAC CTACGAGGAG ATCAGGGACA CCTACCCTGA GGAGTATGCG
CTGCGGGAGC AGGACAAGTA CTATTACCGC TACCCCACCG GGGAGTCCTA CCAGGACCTG
GTCCAGCGCT TGGAGCCAGT GATCATGGAG CTGGAGCGGC AGGAGAATGT GCTGGTCATC
TGCCACCAGG CCGTCCTGCG CTGCCTGCTT GCCTACTTCC TGGATAAGAG TGCAGAGGAG
ATGCCCTACC TGAAATGCCC TCTTCACACC GTCCTGAAAC TGACGCCTGT CGCTTATGGC
TGCCGTGTGG AATCCATCTA CCTGAACGTG GAGTCCGTCT GCACACACCG GGAGAGGTCA
GAGGATGCAA AGAAGGGACC TAACCCGCTC ATGAGACGCA ATAGTGTCAC CCCGCTAGCC
AGCCCCGAAC CCACCAAAAA GCCTCGCATC AACAGCTTTG AGGAGCATGT GGCCTCCACC
TCGGCCGCCC TGCCCAGCTG CCTGCCCCCG GAGGTGCCCA CGCAGCTGCC TGGACAAAAC
ATGAAAGGCT CCCGGAGCAG CGCTGACTCC TCCAGGAAAC ACTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001269559.1
CDS64..1668
Misc Feature(1)19..21(+)
Misc Feature(2)184..843(+)
Misc Feature(3)847..1407(+)
Misc Feature(4)862..1272(+)
Exon (1)1..181
Gene:PFKFB3
Gene Synonym:
Exon (2)182..307
Gene:PFKFB3
Gene Synonym:
Exon (3)308..404
Gene:PFKFB3
Gene Synonym:
Exon (4)405..471
Gene:PFKFB3
Gene Synonym:
Exon (5)472..546
Gene:PFKFB3
Gene Synonym:
Exon (6)547..603
Gene:PFKFB3
Gene Synonym:
Exon (7)604..728
Gene:PFKFB3
Gene Synonym:
Exon (8)729..936
Gene:PFKFB3
Gene Synonym:
Exon (9)937..1083
Gene:PFKFB3
Gene Synonym:
Exon (10)1084..1188
Gene:PFKFB3
Gene Synonym:
Exon (11)1189..1318
Gene:PFKFB3
Gene Synonym:
Exon (12)1319..1381
Gene:PFKFB3
Gene Synonym:
Exon (13)1382..1446
Gene:PFKFB3
Gene Synonym:
Exon (14)1447..1620
Gene:PFKFB3
Gene Synonym:
Exon (15)1621..4271
Gene:PFKFB3
Gene Synonym:
Translation
Position Chain Variation Link
1 1 a, c dbSNP:531002021
11 11 c, t dbSNP:764773110
24 24 a, c, g dbSNP:530198466
27 27 g, t dbSNP:777326651
43 43 a, c dbSNP:548671826
53 53 g, t dbSNP:765472700
58 58 a, g dbSNP:752840290
60 60 a, g dbSNP:7074082
61 61 c, g dbSNP:764257198
64 64 a, t dbSNP:778814373
67 67 a, g dbSNP:751323083
105 105 -, g dbSNP:751358483
106 106 a, g dbSNP:757054514
133 133 c, g dbSNP:781148937
147 147 c, t dbSNP:182290931
151 151 a, g dbSNP:559034540
155 155 c, t dbSNP:570845140
162 162 c, t dbSNP:188663693
177 177 -, c dbSNP:761000535
182 182 c, t dbSNP:758028195
188 188 a, g dbSNP:763805870
207 207 a, c dbSNP:567913373
208 208 c, t dbSNP:756538480
210 210 a, c dbSNP:528993171
213 213 c, t dbSNP:371698685
214 214 a, g dbSNP:200349885
219 219 c, t dbSNP:779499668
220 220 a, g dbSNP:142603218
237 237 c, t dbSNP:146319524
238 238 a, g dbSNP:759203021
241 241 c, t dbSNP:747176453
267 267 -, gctgactc dbSNP:747356467
273 273 g, t dbSNP:138221143
279 279 c, t dbSNP:776247912
280 280 c, t dbSNP:567196228
285 285 c, t dbSNP:765060646
293 293 a, g dbSNP:775535420
295 295 a, g dbSNP:375018306
297 297 c, t dbSNP:763750903
308 308 c, t dbSNP:781263393
315 315 c, t dbSNP:746156494
316 316 a, g dbSNP:181651478
318 318 -, g dbSNP:780889322
318 318 c, t dbSNP:374201933
331 331 c, t dbSNP:749129357
347 347 a, g dbSNP:547569491
350 350 a, g dbSNP:774481512
352 352 a, g dbSNP:376208847
370 370 c, t dbSNP:771681077
372 372 c, t dbSNP:772727967
376 376 a, g dbSNP:760318737
378 378 c, t dbSNP:199980015
379 379 a, c dbSNP:753009870
385 385 a, g dbSNP:149555675
392 392 c, t dbSNP:764552151
400 400 c, t dbSNP:201889586
401 401 a, g dbSNP:36005730
404 404 a, g dbSNP:757268684
414 414 c, t dbSNP:542439439
416 416 c, t dbSNP:773635607
419 419 c, g dbSNP:760834564
421 421 g, t dbSNP:766625430
436 436 a, c dbSNP:753980509
437 437 a, g dbSNP:765641319
449 449 a, c, t dbSNP:765399775
450 450 a, g dbSNP:750805171
454 454 c, g, t dbSNP:763367903
471 471 a, g dbSNP:757197649
477 477 c, t dbSNP:748952044
491 491 c, t dbSNP:768370594
511 511 a, g dbSNP:777933166
530 530 a, g dbSNP:747409261
543 543 c, t dbSNP:138054261
548 548 c, t dbSNP:144243977
549 549 a, g dbSNP:34969226
558 558 c, t dbSNP:754555836
559 559 a, g dbSNP:773437490
562 562 c, t dbSNP:576701454
563 563 c, t dbSNP:764975677
564 564 a, g dbSNP:79668213
565 565 a, g dbSNP:758136512
566 566 c, t dbSNP:758712431
570 570 c, t dbSNP:746271169
571 571 a, g dbSNP:756527360
573 573 c, t dbSNP:573415324
574 574 a, g dbSNP:749299504
591 591 c, g dbSNP:374769986
598 598 a, g dbSNP:776918283
602 602 c, t dbSNP:774547245
609 609 a, g, t dbSNP:201048797
613 613 a, g dbSNP:373530404
617 617 a, c dbSNP:769828653
634 634 a, g dbSNP:775606147
641 641 a, t dbSNP:762545765
644 644 c, t dbSNP:768274515
645 645 a, g dbSNP:377330736
655 655 a, g dbSNP:761465902
659 659 a, g dbSNP:767107653
660 660 c, t dbSNP:545085751
677 677