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PFKM cDNA ORF clone, Homo sapiens (human)

Gene Symbol PFKM
Entrez Gene ID 5213
Full Name phosphofructokinase, muscle
Synonyms ATP-PFK, GSD7, PFK-1, PFK1, PFKA, PFKX, PPP1R122
General protein information
Preferred Names
6-phosphofructokinase, muscle type
Names
6-phosphofructokinase, muscle type
PFK-A
phosphohexokinase
phosphofructokinase 1
phosphofructokinase-M
6-phosphofructo-1-kinase
phosphofructo-1-kinase isozyme A
phosphofructokinase, polypeptide X
NP_000280.1
NP_001160158.1
NP_001160159.1
NP_001160160.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q13.3

Summary Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]. lac of sum
Disorder MIM:

610681

Disorder Html: Glycogen storage disease VII, 232800 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011538487 XP_011536789 ATP-dependent 6-phosphofructokinase, muscle type isoform X3
XM_005268975 XP_005269032 ATP-dependent 6-phosphofructokinase, muscle type isoform X4
XM_005268974 XP_005269031 ATP-dependent 6-phosphofructokinase, muscle type isoform X4
XM_005268976 XP_005269033 ATP-dependent 6-phosphofructokinase, muscle type isoform X4
XM_005268977 XP_005269034 ATP-dependent 6-phosphofructokinase, muscle type isoform X1
XM_005268978 XP_005269035 ATP-dependent 6-phosphofructokinase, muscle type isoform X1
XM_005268979 XP_005269036 ATP-dependent 6-phosphofructokinase, muscle type isoform X1
XM_011538488 XP_011536790 ATP-dependent 6-phosphofructokinase, muscle type isoform X2
NM_001166686 NP_001160158 ATP-dependent 6-phosphofructokinase, muscle type isoform 1
NM_000289 NP_000280 ATP-dependent 6-phosphofructokinase, muscle type isoform 2
NM_001166687 NP_001160159 ATP-dependent 6-phosphofructokinase, muscle type isoform 2
NM_001166688 NP_001160160 ATP-dependent 6-phosphofructokinase, muscle type isoform 2



Homo sapiens (human) PFKM NP_001160158.1
Pan troglodytes (chimpanzee) PFKM XP_509029.2
Macaca mulatta (Rhesus monkey) PFKM XP_001096244.1
Canis lupus familiaris (dog) PFKM NP_001003199.1
Bos taurus (cattle) PFKM NP_001068736.1
Mus musculus (house mouse) Pfkm NP_067489.3
Rattus norvegicus (Norway rat) Pfkm NP_113903.1
Gallus gallus (chicken) PFKM NP_989554.2
Danio rerio (zebrafish) pfkmb NP_001082886.1
Danio rerio (zebrafish) pfkma NP_001004575.1
Drosophila melanogaster (fruit fly) Pfk NP_724891.2
Caenorhabditis elegans Y71H10A.1 NP_741738.1
Xenopus (Silurana) tropicalis (western clawed frog) pfkm XP_004911968.1


Related articles in PubMed

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PFKM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFKM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu59637 XM_011538487 PREDICTED: Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu46638 XM_005268975 PREDICTED: Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu46638 XM_005268974 PREDICTED: Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu46638 XM_005268976 PREDICTED: Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu28127 XM_005268977 PREDICTED: Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu28127 XM_005268978 PREDICTED: Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu28127 XM_005268979 PREDICTED: Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu28128
XM_011538488 PREDICTED: Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu28127 NM_001166686 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu28128
NM_000289 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu28128
NM_001166687 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu28128
NM_001166688 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu59637
Clone ID Related Accession (Same CDS sequence) XM_011538487
Accession Version XM_011538487.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2559bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA
GACATCAAGA ATGGCAGCAG GATGGGTGTG GAAGCAGTGA TGGCACTTTT GGAGGGGACC
CCAGATACCC CAGCCTGTGT AGTGAGCCTC TCTGGTAACC AGGCTGTGCG CCTGCCCCTC
ATGGAATGTG TCCAGGTGAC CAAAGATGTG ACCAAGGCCA TGGATGAGAA GAAATTTGAC
GAAGCCCTGA AGCTGAGAGG CCGGAGCTTC ATGAACAACT GGGAGGTGTA CAAGCTTCTA
GCTCATGTCA GACCCCCGGT ATCTAAGAGT GGTTCGCACA CAGTGGCTGT GATGAACGTG
GGGGCTCCGG CTGCAGGCAT GAATGCTGCT GTTCGCTCCA CTGTGAGGAT TGGCCTTATC
CAGGGCAACC GAGTGCTCGT TGTCCATGAT GGTTTCGAGG GCCTGGCCAA GGGGCAGATA
GAGGAAGCTG GCTGGAGCTA TGTTGGGGGC TGGACTGGCC AAGGTGGCTC TAAACTTGGG
ACTAAAAGGA CTCTACCCAA GAAGAGCTTT GAACAGATCA GTGCCAATAT AACTAAGTTT
AACATTCAGG GCCTTGTCAT CATTGGGGGC TTTGAGGCTT ACACAGGGGG CCTGGAACTG
ATGGAGGGCA GGAAGCAGTT TGATGAGCTC TGCATCCCAT TTGTGGTCAT TCCTGCTACA
GTCTCCAACA ATGTCCCTGG CTCAGACTTC AGCGTTGGGG CTGACACAGC ACTCAATACT
ATCTGCACAA CCTGTGACCG CATCAAGCAG TCAGCAGCTG GCACCAAGCG TCGGGTGTTT
ATCATTGAGA CTATGGGTGG CTACTGTGGC TACCTGGCTA CCATGGCTGG ACTGGCAGCT
GGGGCCGATG CTGCCTACAT TTTTGAGGAG CCCTTCACCA TTCGAGACCT GCAGGCAAAT
GTTGAACATC TGGTGCAAAA GATGAAAACA ACTGTGAAAA GGGGCTTGGT GTTAAGGAAT
GAAAAGTGCA ATGAGAACTA TACCACTGAC TTCATTTTCA ACCTGTACTC TGAGGAGGGG
AAGGGCATCT TCGACAGCAG GAAGAATGTG CTTGGTCACA TGCAGCAGGG TGGGAGCCCA
ACCCCATTTG ATAGGAATTT TGCCACTAAG ATGGGCGCCA AGGCTATGAA CTGGATGTCT
GGGAAAATCA AAGAGAGTTA CCGTAATGGG CGGATCTTTG CCAATACTCC AGATTCGGGC
TGTGTTCTGG GGATGCGTAA GAGGGCTCTG GTCTTCCAAC CAGTGGCTGA GCTGAAGGAC
CAGACAGATT TTGAGCATCG AATCCCCAAG GAACAGTGGT GGCTGAAACT GAGGCCCATC
CTCAAAATCC TAGCCAAGTA CGAGATTGAC TTGGACACTT CAGACCATGC CCACCTGGAG
CACATCACCC GGAAGCGGTC CGGGGAAGCT GCCGTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011536789.1
CDS10..2568
Misc Feature(1)355..2547(+)
Misc Feature(2)364..2490(+)
Translation

Target ORF information:

RefSeq Version XM_011538487
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011538487

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA
GACATCAAGA ATGGCAGCAG GATGGGTGTG GAAGCAGTGA TGGCACTTTT GGAGGGGACC
CCAGATACCC CAGCCTGTGT AGTGAGCCTC TCTGGTAACC AGGCTGTGCG CCTGCCCCTC
ATGGAATGTG TCCAGGTGAC CAAAGATGTG ACCAAGGCCA TGGATGAGAA GAAATTTGAC
GAAGCCCTGA AGCTGAGAGG CCGGAGCTTC ATGAACAACT GGGAGGTGTA CAAGCTTCTA
GCTCATGTCA GACCCCCGGT ATCTAAGAGT GGTTCGCACA CAGTGGCTGT GATGAACGTG
GGGGCTCCGG CTGCAGGCAT GAATGCTGCT GTTCGCTCCA CTGTGAGGAT TGGCCTTATC
CAGGGCAACC GAGTGCTCGT TGTCCATGAT GGTTTCGAGG GCCTGGCCAA GGGGCAGATA
GAGGAAGCTG GCTGGAGCTA TGTTGGGGGC TGGACTGGCC AAGGTGGCTC TAAACTTGGG
ACTAAAAGGA CTCTACCCAA GAAGAGCTTT GAACAGATCA GTGCCAATAT AACTAAGTTT
AACATTCAGG GCCTTGTCAT CATTGGGGGC TTTGAGGCTT ACACAGGGGG CCTGGAACTG
ATGGAGGGCA GGAAGCAGTT TGATGAGCTC TGCATCCCAT TTGTGGTCAT TCCTGCTACA
GTCTCCAACA ATGTCCCTGG CTCAGACTTC AGCGTTGGGG CTGACACAGC ACTCAATACT
ATCTGCACAA CCTGTGACCG CATCAAGCAG TCAGCAGCTG GCACCAAGCG TCGGGTGTTT
ATCATTGAGA CTATGGGTGG CTACTGTGGC TACCTGGCTA CCATGGCTGG ACTGGCAGCT
GGGGCCGATG CTGCCTACAT TTTTGAGGAG CCCTTCACCA TTCGAGACCT GCAGGCAAAT
GTTGAACATC TGGTGCAAAA GATGAAAACA ACTGTGAAAA GGGGCTTGGT GTTAAGGAAT
GAAAAGTGCA ATGAGAACTA TACCACTGAC TTCATTTTCA ACCTGTACTC TGAGGAGGGG
AAGGGCATCT TCGACAGCAG GAAGAATGTG CTTGGTCACA TGCAGCAGGG TGGGAGCCCA
ACCCCATTTG ATAGGAATTT TGCCACTAAG ATGGGCGCCA AGGCTATGAA CTGGATGTCT
GGGAAAATCA AAGAGAGTTA CCGTAATGGG CGGATCTTTG CCAATACTCC AGATTCGGGC
TGTGTTCTGG GGATGCGTAA GAGGGCTCTG GTCTTCCAAC CAGTGGCTGA GCTGAAGGAC
CAGACAGATT TTGAGCATCG AATCCCCAAG GAACAGTGGT GGCTGAAACT GAGGCCCATC
CTCAAAATCC TAGCCAAGTA CGAGATTGAC TTGGACACTT CAGACCATGC CCACCTGGAG
CACATCACCC GGAAGCGGTC CGGGGAAGCT GCCGTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu46638
Clone ID Related Accession (Same CDS sequence) XM_005268974 , XM_005268975 , XM_005268976
Accession Version XM_005268975.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005269032.1
CDS215..2866
Misc Feature(1)560..2845(+)
Misc Feature(2)569..2788(+)
Translation

Target ORF information:

RefSeq Version XM_005268975
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005268975

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu46638
Clone ID Related Accession (Same CDS sequence) XM_005268974 , XM_005268975 , XM_005268976
Accession Version XM_005268974.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005269031.1
CDS76..2727
Misc Feature(1)421..2706(+)
Misc Feature(2)430..2649(+)
Translation

Target ORF information:

RefSeq Version XM_005268974
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005268974

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu46638
Clone ID Related Accession (Same CDS sequence) XM_005268974 , XM_005268975 , XM_005268976
Accession Version XM_005268976.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530400465. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005269033.1
CDS120..2771
Misc Feature(1)465..2750(+)
Misc Feature(2)474..2693(+)
Translation

Target ORF information:

RefSeq Version XM_005268976
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005268976

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28127
Clone ID Related Accession (Same CDS sequence) NM_001166686 , XM_005268977 , XM_005268978 , XM_005268979
Accession Version XM_005268977.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2556bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005269034.1
CDS158..2713
Misc Feature(1)407..2692(+)
Misc Feature(2)416..2635(+)
Translation

Target ORF information:

RefSeq Version XM_005268977
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005268977

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28127
Clone ID Related Accession (Same CDS sequence) NM_001166686 , XM_005268977 , XM_005268978 , XM_005268979
Accession Version XM_005268978.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2556bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced gi:530400469. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005269035.1
CDS71..2626
Misc Feature(1)320..2605(+)
Misc Feature(2)329..2548(+)
Translation

Target ORF information:

RefSeq Version XM_005268978
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005268978

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28127
Clone ID Related Accession (Same CDS sequence) NM_001166686 , XM_005268977 , XM_005268978 , XM_005268979
Accession Version XM_005268979.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2556bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005269036.1
CDS158..2713
Misc Feature(1)407..2692(+)
Misc Feature(2)416..2635(+)
Translation

Target ORF information:

RefSeq Version XM_005268979
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005268979

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28128
Clone ID Related Accession (Same CDS sequence) NM_000289 , NM_001166687 , NM_001166688 , XM_011538488
Accession Version XM_011538488.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2343bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011536790.1
CDS27..2369
Misc Feature(1)63..2348(+)
Misc Feature(2)72..2291(+)
Translation

Target ORF information:

RefSeq Version XM_011538488
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011538488

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28127
Clone ID Related Accession (Same CDS sequence) NM_001166686 , XM_005268977 , XM_005268978 , XM_005268979
Accession Version NM_001166686.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2556bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB081387.1, BC021203.2, BC000534.2 and BU683561.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longest transcript (also known as TE-PFKM) and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK126229.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001160158.1
CDS275..2830
Misc Feature(1)263..265(+)
Misc Feature(2)524..2809(+)
Misc Feature(3)533..2752(+)
Exon (1)1..265
Gene:PFKM
Gene Synonym:
Exon (2)266..356
Gene:PFKM
Gene Synonym:
Exon (3)357..479
Gene:PFKM
Gene Synonym:
Exon (4)480..572
Gene:PFKM
Gene Synonym:
Exon (5)573..646
Gene:PFKM
Gene Synonym:
Exon (6)647..724
Gene:PFKM
Gene Synonym:
Exon (7)725..914
Gene:PFKM
Gene Synonym:
Exon (8)915..1080
Gene:PFKM
Gene Synonym:
Exon (9)1081..1125
Gene:PFKM
Gene Synonym:
Exon (10)1126..1234
Gene:PFKM
Gene Synonym:
Exon (11)1235..1330
Gene:PFKM
Gene Synonym:
Exon (12)1331..1423
Gene:PFKM
Gene Synonym:
Exon (13)1424..1549
Gene:PFKM
Gene Synonym:
Exon (14)1550..1614
Gene:PFKM
Gene Synonym:
Exon (15)1615..1678
Gene:PFKM
Gene Synonym:
Exon (16)1679..1828
Gene:PFKM
Gene Synonym:
Exon (17)1829..1899
Gene:PFKM
Gene Synonym:
Exon (18)1900..1987
Gene:PFKM
Gene Synonym:
Exon (19)1988..2140
Gene:PFKM
Gene Synonym:
Exon (20)2141..2305
Gene:PFKM
Gene Synonym:
Exon (21)2306..2367
Gene:PFKM
Gene Synonym:
Exon (22)2368..2479
Gene:PFKM
Gene Synonym:
Exon (23)2480..2579
Gene:PFKM
Gene Synonym:
Exon (24)2580..2685
Gene:PFKM
Gene Synonym:
Exon (25)2686..3526
Gene:PFKM
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001166686
Organism Homo sapiens (human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001166686

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28128
Clone ID Related Accession (Same CDS sequence) NM_000289 , NM_001166687 , NM_001166688 , XM_011538488
Accession Version NM_000289.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2343bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA525437.1, BC021203.2, BC000534.2 and BU683561.1. On Nov 11, 2009 this sequence version replaced gi:171543862. Transcript Variant: This variant (4, also known as type C) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2, 3 and 4 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M26066.1, BC021203.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000280.1
CDS186..2528
Misc Feature(1)189..1355(+)
Misc Feature(2)189..191(+)
Misc Feature(3)222..2507(+)
Misc Feature(4)231..2450(+)
Misc Feature(5)675..683(+)
Misc Feature(6)807..815(+)
Misc Feature(7)1077..1088(+)
Misc Feature(8)1314..1316(+)
Misc Feature(9)1356..1388(+)
Misc Feature(10)1389..2525(+)
Misc Feature(11)1767..1781(+)
Misc Feature(12)1902..1910(+)
Misc Feature(13)2166..2177(+)
Misc Feature(14)2184..2186(+)
Misc Feature(15)2508..2510(+)
Misc Feature(16)2508..2510(+)
Exon (1)1..177
Gene:PFKM
Gene Synonym:
Exon (2)178..270
Gene:PFKM
Gene Synonym:
Exon (3)271..344
Gene:PFKM
Gene Synonym:
Exon (4)345..422
Gene:PFKM
Gene Synonym:
Exon (5)423..612
Gene:PFKM
Gene Synonym:
Exon (6)613..778
Gene:PFKM
Gene Synonym:
Exon (7)779..823
Gene:PFKM
Gene Synonym:
Exon (8)824..932
Gene:PFKM
Gene Synonym:
Exon (9)933..1028
Gene:PFKM
Gene Synonym:
Exon (10)1029..1121
Gene:PFKM
Gene Synonym:
Exon (11)1122..1247
Gene:PFKM
Gene Synonym:
Exon (12)1248..1312
Gene:PFKM
Gene Synonym:
Exon (13)1313..1376
Gene:PFKM
Gene Synonym:
Exon (14)1377..1526
Gene:PFKM
Gene Synonym:
Exon (15)1527..1597
Gene:PFKM
Gene Synonym:
Exon (16)1598..1685
Gene:PFKM
Gene Synonym:
Exon (17)1686..1838
Gene:PFKM
Gene Synonym:
Exon (18)1839..2003
Gene:PFKM
Gene Synonym:
Exon (19)2004..2065
Gene:PFKM
Gene Synonym:
Exon (20)2066..2177
Gene:PFKM
Gene Synonym:
Exon (21)2178..2277
Gene:PFKM
Gene Synonym:
Exon (22)2278..2383
Gene:PFKM
Gene Synonym:
Exon (23)2384..3224
Gene:PFKM
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000289
Organism Homo sapiens (human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000289

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28128
Clone ID Related Accession (Same CDS sequence) NM_000289 , NM_001166687 , NM_001166688 , XM_011538488
Accession Version NM_001166687.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2343bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA560355.1, Y00698.1, BC000534.2 and BU683561.1. Transcript Variant: This variant (2, also known as type A) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2, 3 and 4 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y00698.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001160159.1
CDS40..2382
Misc Feature(1)28..30(+)
Misc Feature(2)43..1209(+)
Misc Feature(3)43..45(+)
Misc Feature(4)76..2361(+)
Misc Feature(5)85..2304(+)
Misc Feature(6)529..537(+)
Misc Feature(7)661..669(+)
Misc Feature(8)931..942(+)
Misc Feature(9)1210..1242(+)
Misc Feature(10)1243..2379(+)
Misc Feature(11)1621..1635(+)
Misc Feature(12)1756..1764(+)
Misc Feature(13)2020..2031(+)
Misc Feature(14)2038..2040(+)
Misc Feature(15)2362..2364(+)
Exon (1)1..31
Gene:PFKM
Gene Synonym:
Exon (2)32..124
Gene:PFKM
Gene Synonym:
Exon (3)125..198
Gene:PFKM
Gene Synonym:
Exon (4)199..276
Gene:PFKM
Gene Synonym:
Exon (5)277..466
Gene:PFKM
Gene Synonym:
Exon (6)467..632
Gene:PFKM
Gene Synonym:
Exon (7)633..677
Gene:PFKM
Gene Synonym:
Exon (8)678..786
Gene:PFKM
Gene Synonym:
Exon (9)787..882
Gene:PFKM
Gene Synonym:
Exon (10)883..975
Gene:PFKM
Gene Synonym:
Exon (11)976..1101
Gene:PFKM
Gene Synonym:
Exon (12)1102..1166
Gene:PFKM
Gene Synonym:
Exon (13)1167..1230
Gene:PFKM
Gene Synonym:
Exon (14)1231..1380
Gene:PFKM
Gene Synonym:
Exon (15)1381..1451
Gene:PFKM
Gene Synonym:
Exon (16)1452..1539
Gene:PFKM
Gene Synonym:
Exon (17)1540..1692
Gene:PFKM
Gene Synonym:
Exon (18)1693..1857
Gene:PFKM
Gene Synonym:
Exon (19)1858..1919
Gene:PFKM
Gene Synonym:
Exon (20)1920..2031
Gene:PFKM
Gene Synonym:
Exon (21)2032..2131
Gene:PFKM
Gene Synonym:
Exon (22)2132..2237
Gene:PFKM
Gene Synonym:
Exon (23)2238..3078
Gene:PFKM
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001166687
Organism Homo sapiens (human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001166687

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28128
Clone ID Related Accession (Same CDS sequence) NM_000289 , NM_001166687 , NM_001166688 , XM_011538488
Accession Version NM_001166688.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2343bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA761651.1, BC000534.2 and BU683561.1. Transcript Variant: This variant (3, also known as type B) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2, 3 and 4 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000534.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001160160.1
CDS129..2471
Misc Feature(1)111..113(+)
Misc Feature(2)132..1298(+)
Misc Feature(3)132..134(+)
Misc Feature(4)165..2450(+)
Misc Feature(5)174..2393(+)
Misc Feature(6)618..626(+)
Misc Feature(7)750..758(+)
Misc Feature(8)1020..1031(+)
Misc Feature(9)1299..1331(+)
Misc Feature(10)1332..2468(+)
Misc Feature(11)1710..1724(+)
Misc Feature(12)1845..1853(+)
Misc Feature(13)2109..2120(+)
Misc Feature(14)2127..2129(+)
Misc Feature(15)2451..2453(+)
Exon (1)1..213
Gene:PFKM
Gene Synonym:
Exon (2)214..287
Gene:PFKM
Gene Synonym:
Exon (3)288..365
Gene:PFKM
Gene Synonym:
Exon (4)366..555
Gene:PFKM
Gene Synonym:
Exon (5)556..721
Gene:PFKM
Gene Synonym:
Exon (6)722..766
Gene:PFKM
Gene Synonym:
Exon (7)767..875
Gene:PFKM
Gene Synonym:
Exon (8)876..971
Gene:PFKM
Gene Synonym:
Exon (9)972..1064
Gene:PFKM
Gene Synonym:
Exon (10)1065..1190
Gene:PFKM
Gene Synonym:
Exon (11)1191..1255
Gene:PFKM
Gene Synonym:
Exon (12)1256..1319
Gene:PFKM
Gene Synonym:
Exon (13)1320..1469
Gene:PFKM
Gene Synonym:
Exon (14)1470..1540
Gene:PFKM
Gene Synonym:
Exon (15)1541..1628
Gene:PFKM
Gene Synonym:
Exon (16)1629..1781
Gene:PFKM
Gene Synonym:
Exon (17)1782..1946
Gene:PFKM
Gene Synonym:
Exon (18)1947..2008
Gene:PFKM
Gene Synonym:
Exon (19)2009..2120
Gene:PFKM
Gene Synonym:
Exon (20)2121..2220
Gene:PFKM
Gene Synonym:
Exon (21)2221..2326
Gene:PFKM
Gene Synonym:
Exon (22)2327..3167
Gene:PFKM
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001166688
Organism Homo sapiens (human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001166688

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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