Email to GenScript

PGK1 phosphoglycerate kinase 1 [Homo sapiens (human)]

Gene Symbol PGK1
Entrez Gene ID 5230
Full Name phosphoglycerate kinase 1
Synonyms HEL-S-68p, MIG10, PGKA
General protein information
Preferred Names
phosphoglycerate kinase 1
Names
phosphoglycerate kinase 1
PRP 2
primer recognition protein 2
cell migration-inducing gene 10 protein
NP_000282.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xq13.3

Summary The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]. lac of sum
Disorder MIM:

311800

Disorder Html: Phosphoglycerate kinase 1 deficiency, 300653 (3)





The following PGK1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PGK1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19088 NM_000291 Homo sapiens phosphoglycerate kinase 1 (PGK1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$379

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu19088
Accession Version NM_000291.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1254bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product phosphoglycerate kinase 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC336483.1, BC103752.1 and BU677171.1. This sequence is a reference standard in the RefSeqGene project. On Apr 18, 2008 this sequence version replaced gi:22095338. Summary: The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 2324090, 11130727 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AB062432.1, BC113568.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCGCTTT CTAACAAGCT GACGCTGGAC AAGCTGGACG TTAAAGGGAA GCGGGTCGTT 
ATGAGAGTCG ACTTCAATGT TCCTATGAAG AACAACCAGA TAACAAACAA CCAGAGGATT
AAGGCTGCTG TCCCAAGCAT CAAATTCTGC TTGGACAATG GAGCCAAGTC GGTAGTCCTT
ATGAGCCACC TAGGCCGGCC TGATGGTGTG CCCATGCCTG ACAAGTACTC CTTAGAGCCA
GTTGCTGTAG AACTCAAATC TCTGCTGGGC AAGGATGTTC TGTTCTTGAA GGACTGTGTA
GGCCCAGAAG TGGAGAAAGC CTGTGCCAAC CCAGCTGCTG GGTCTGTCAT CCTGCTGGAG
AACCTCCGCT TTCATGTGGA GGAAGAAGGG AAGGGAAAAG ATGCTTCTGG GAACAAGGTT
AAAGCCGAGC CAGCCAAAAT AGAAGCTTTC CGAGCTTCAC TTTCCAAGCT AGGGGATGTC
TATGTCAATG ATGCTTTTGG CACTGCTCAC AGAGCCCACA GCTCCATGGT AGGAGTCAAT
CTGCCACAGA AGGCTGGTGG GTTTTTGATG AAGAAGGAGC TGAACTACTT TGCAAAGGCC
TTGGAGAGCC CAGAGCGACC CTTCCTGGCC ATCCTGGGCG GAGCTAAAGT TGCAGACAAG
ATCCAGCTCA TCAATAATAT GCTGGACAAA GTCAATGAGA TGATTATTGG TGGTGGAATG
GCTTTTACCT TCCTTAAGGT GCTCAACAAC ATGGAGATTG GCACTTCTCT GTTTGATGAA
GAGGGAGCCA AGATTGTCAA AGACCTAATG TCCAAAGCTG AGAAGAATGG TGTGAAGATT
ACCTTGCCTG TTGACTTTGT CACTGCTGAC AAGTTTGATG AGAATGCCAA GACTGGCCAA
GCCACTGTGG CTTCTGGCAT ACCTGCTGGC TGGATGGGCT TGGACTGTGG TCCTGAAAGC
AGCAAGAAGT ATGCTGAGGC TGTCACTCGG GCTAAGCAGA TTGTGTGGAA TGGTCCTGTG
GGGGTATTTG AATGGGAAGC TTTTGCCCGG GGAACCAAAG CTCTCATGGA TGAGGTGGTG
AAAGCCACTT CTAGGGGCTG CATCACCATC ATAGGTGGTG GAGACACTGC CACTTGCTGT
GCCAAATGGA ACACGGAGGA TAAAGTCAGC CATGTGAGCA CTGGGGGTGG TGCCAGTTTG
GAGCTCCTGG AAGGTAAAGT CCTTCCTGGG GTGGATGCTC TCAGCAATAT TTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000282.1
CDS173..1426
Misc Feature(1)50..52(+)
Misc Feature(2)176..178(+)
Misc Feature(3)197..1420(+)
Misc Feature(4)203..205(+)
Misc Feature(5)242..541(+)
Misc Feature(6)242..250(+)
Misc Feature(7)314..316(+)
Misc Feature(8)359..370(+)
Misc Feature(9)395..397(+)
Misc Feature(10)428..430(+)
Misc Feature(11)461..463(+)
Misc Feature(12)563..565(+)
Misc Feature(13)563..565(+)
Misc Feature(14)608..610(+)
Misc Feature(15)758..760(+)
Misc Feature(16)758..760(+)
Misc Feature(17)767..769(+)
Misc Feature(18)773..1354(+)
Misc Feature(19)779..781(+)
Misc Feature(20)779..781(+)
Misc Feature(21)887..1300(+)
Misc Feature(22)971..973(+)
Misc Feature(23)1043..1045(+)
Misc Feature(24)1295..1297(+)
Exon (1)1..237
Gene:PGK1
Gene Synonym:
Exon (2)238..288
Gene:PGK1
Gene Synonym:
Exon (3)289..444
Gene:PGK1
Gene Synonym:
Exon (4)445..589
Gene:PGK1
Gene Synonym:
Exon (5)590..693
Gene:PGK1
Gene Synonym:
Exon (6)694..813
Gene:PGK1
Gene Synonym:
Exon (7)814..928
Gene:PGK1
Gene Synonym:
Exon (8)929..1108
Gene:PGK1
Gene Synonym:
Exon (9)1109..1286
Gene:PGK1
Gene Synonym:
Exon (10)1287..1385
Gene:PGK1
Gene Synonym:
Exon (11)1386..2423
Gene:PGK1
Gene Synonym:
Translation
Position Chain Variation Link
103 103 g, t dbSNP:112702955
312 312 a, t dbSNP:137852536
315 315 a, g dbSNP:201833700
342 342 c, t dbSNP:200132648
343 343 a, g, t dbSNP:78165041
370 370 c, g dbSNP:201498142
389 389 c, t dbSNP:148077298
403 403 c, t dbSNP:199906447
409 409 a, g dbSNP:201217519
413 413 g, t dbSNP:202138276
420 420 c, t dbSNP:138851144
435 435 c, t dbSNP:137852531
590 590 a, g dbSNP:201024012
598 598 c, t dbSNP:201516932
600 600 a, t dbSNP:200869673
609 609 a, t dbSNP:200372952
645 645 g, t dbSNP:137852532
658 658 c, g dbSNP:201708335
660 660 a, g dbSNP:185071030
663 663 a, t dbSNP:137852538
746 746 -, aag dbSNP:431905502
789 789 c, g dbSNP:137852529
846 846 a, g dbSNP:143031509
907 907 a, t dbSNP:202023023
930 930 c, t dbSNP:137852534
933 933 c, g dbSNP:148399096
968 968 atg, gtc dbSNP:431905501
974 974 a, g dbSNP:137852528
1004 1004 a, g dbSNP:199571497
1026 1026 a, t dbSNP:137852535
1059 1059 c, t dbSNP:200543437
1070 1070 a, c, g dbSNP:201707198
1081 1081 c, g dbSNP:142525614
1086 1086 c, g dbSNP:200778983
1101 1101 a, g dbSNP:201238498
1118 1118 c, t dbSNP:137852533
1131 1131 a, g dbSNP:137852537
1227 1227 a, c dbSNP:137852530
1286 1286 a, g dbSNP:727504084
1304 1304 a, c dbSNP:137852539
1437 1437 c, t dbSNP:201597074
2378 2378 c, t dbSNP:199863795

Target ORF information:

RefSeq Version NM_000291
Organism Homo sapiens (human)
Definition Homo sapiens phosphoglycerate kinase 1 (PGK1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000291

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCGCTTT CTAACAAGCT GACGCTGGAC AAGCTGGACG TTAAAGGGAA GCGGGTCGTT 
ATGAGAGTCG ACTTCAATGT TCCTATGAAG AACAACCAGA TAACAAACAA CCAGAGGATT
AAGGCTGCTG TCCCAAGCAT CAAATTCTGC TTGGACAATG GAGCCAAGTC GGTAGTCCTT
ATGAGCCACC TAGGCCGGCC TGATGGTGTG CCCATGCCTG ACAAGTACTC CTTAGAGCCA
GTTGCTGTAG AACTCAAATC TCTGCTGGGC AAGGATGTTC TGTTCTTGAA GGACTGTGTA
GGCCCAGAAG TGGAGAAAGC CTGTGCCAAC CCAGCTGCTG GGTCTGTCAT CCTGCTGGAG
AACCTCCGCT TTCATGTGGA GGAAGAAGGG AAGGGAAAAG ATGCTTCTGG GAACAAGGTT
AAAGCCGAGC CAGCCAAAAT AGAAGCTTTC CGAGCTTCAC TTTCCAAGCT AGGGGATGTC
TATGTCAATG ATGCTTTTGG CACTGCTCAC AGAGCCCACA GCTCCATGGT AGGAGTCAAT
CTGCCACAGA AGGCTGGTGG GTTTTTGATG AAGAAGGAGC TGAACTACTT TGCAAAGGCC
TTGGAGAGCC CAGAGCGACC CTTCCTGGCC ATCCTGGGCG GAGCTAAAGT TGCAGACAAG
ATCCAGCTCA TCAATAATAT GCTGGACAAA GTCAATGAGA TGATTATTGG TGGTGGAATG
GCTTTTACCT TCCTTAAGGT GCTCAACAAC ATGGAGATTG GCACTTCTCT GTTTGATGAA
GAGGGAGCCA AGATTGTCAA AGACCTAATG TCCAAAGCTG AGAAGAATGG TGTGAAGATT
ACCTTGCCTG TTGACTTTGT CACTGCTGAC AAGTTTGATG AGAATGCCAA GACTGGCCAA
GCCACTGTGG CTTCTGGCAT ACCTGCTGGC TGGATGGGCT TGGACTGTGG TCCTGAAAGC
AGCAAGAAGT ATGCTGAGGC TGTCACTCGG GCTAAGCAGA TTGTGTGGAA TGGTCCTGTG
GGGGTATTTG AATGGGAAGC TTTTGCCCGG GGAACCAAAG CTCTCATGGA TGAGGTGGTG
AAAGCCACTT CTAGGGGCTG CATCACCATC ATAGGTGGTG GAGACACTGC CACTTGCTGT
GCCAAATGGA ACACGGAGGA TAAAGTCAGC CATGTGAGCA CTGGGGGTGG TGCCAGTTTG
GAGCTCCTGG AAGGTAAAGT CCTTCCTGGG GTGGATGCTC TCAGCAATAT TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.