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PHKA1 phosphorylase kinase, alpha 1 (muscle) [Homo sapiens (human)]

Gene Symbol PHKA1
Entrez Gene ID 5255
Full Name phosphorylase kinase, alpha 1 (muscle)
Synonyms PHKA
General protein information
Preferred Names
phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
Names
phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
phosphorylase kinase alpha M subunit
phosphorylase kinase, alpha 1 (muscle), muscle glycogenosis
phosphorylase b kinase regulatory subunit alpha skeletal muscle isoform
NP_001116142.1
NP_001165907.1
NP_002628.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xq12-q13

Summary Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D, also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1.[provided by RefSeq, Feb 2010]. lac of sum
Disorder MIM:

311870

Disorder Html: Muscle glycogenosis, 300559 (3)





The following PHKA1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHKA1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu54173 XM_006724661 PREDICTED: Homo sapiens phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 Quote Price
OHu17843 NM_002637 Homo sapiens phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 Quote Price
OHu16403 NM_001122670 Homo sapiens phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$384.3
$549
OHu16802 NM_001172436 Homo sapiens phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 Quote Price

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu54173
Accession Version XM_006724661.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011651.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578838396. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGAGCC GGAGTAACTC CGGGGTCCGG CTGGACGGCT ACGCTCGACT GGTGCAACAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACT GGCTTGCTTC CAGCCAGCTA TGATCAGAAA
GATGCTTGGG TCCGAGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
TATCGGAAGA ATGCAGACCG GGATGAGGAT AAGGCAAAGG CCTATGAATT GGAGCAGAGT
GTAGTGAAGC TGATGAGAGG ACTACTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
TCCTTCAAAT ATAGTCAGAG TACTAAGGAT AGCCTCCATG CAAAGTACAA CACCAAAACC
TGTGCCACTG TAGTGGGTGA TGATCAATGG GGACACCTGC AGTTGGATGC TACCTCTGTG
TACCTGCTCT TCTTAGCCCA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAT
GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
TTCGGGATAT GGGAACGTGG AGACAAGACC AACCAAGGGA TCTCAGAGTT GAATGCCAGT
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGATCT GTTTGGTGTG
AAAGGTGGGC CTCAATCAGT TATCCATGTC CTGGCTGATG AAGTACAGCA CTGCCAGTCT
ATCCTAAATT CACTACTGCC CCGTGCTTCA ACATCAAAAG AGGTTGATGC TAGTCTACTC
TCAGTGGTTT CCTTCCCTGC CTTTGCAGTA GAGGATAGCC AGTTGGTGGA GCTCACAAAA
CAGGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGCTA
TTTGAAAACA TTGAGTGTGA ATGGCCATTG TTCTGGACAT ACTTTATTCT TGATGGGGTC
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TATAAAGAGG CTCTTGAAGC AGTCCTCATC
AAGGGCAAAA ATGGAGTCCC ACTTCTGCCA GAGCTGTACA GTGTTCCTCC TGACAGGGTC
GATGAAGAAT ATCAGAATCC TCACACTGTG GACCGAGTCC CCATGGGGAA ATTGCCTCAC
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCCCCT
GGAGAAATTG ATCCCCTGAA TCGCAGGTTT TCTACTGTAC CGAAGCCCGA TGTTGTGGTT
CAAGTCTCCA TTCTAGCTGA AACAGAAGAA ATCAAGACCA TTTTGAAGGA CAAGGGAATT
TACGTGGAGA CCATTGCTGA GGTATACCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC
CACATTTATT CCAGCCTAGG ATGCAACAAT AGAATGAAAC TCAGTGGACG ACCCTACAGA
CACATGGGAG TGCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACTTTC
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTG
GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGCGGATGAC AGGCCAGCCC
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACAAG CTTGAATTCA
AGTATCCTGG CAGCACTCCG AAAAATGCAA GATGGGTATT TTGGTGGGGC AAGGGTTCAA
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACACTTGAG CTTCATGGAC
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG ACAACTATGA TTACCTGGAA
TCTGGCAACT GGATGAATGA TTATGATTCA ACCAGTCATG ATGTTCACAT GTATCTTCCT
ACGAAGTTAT TTCAGGCTTC CCGGCCTTCA TTCAACTTAC TTGATTCACC TCATCCCCGA
CAGGAGAACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CCAGTCTGGG
GAGGTGGACT TTAAAGCACT GGTTTTACAG TTGAAGGAGA CCTCAAGCTT ACAGGAACAA
GCTGATATCC TCTATATGCT GTATACTATG AAAGGACCTG ACTGGAACAC TGAATTGTAT
AATGAACGGA GTGCTACAGT GAGAGAGCTT CTTACCGAGC TGTATGGCAA AGTGGGAGAA
ATTCGTCACT GGGGCCTGAT CCGATACATT TCTGGGATCT TAAGGAAGAA AGTGGAAGCA
CTTGATGAGG CCTGCACAGA CCTTCTCTCC CACCAGAAAC ATTTGACAGT AGGACTTCCT
CCAGAACCTC GAGAAAAGAC TATCTCTGCA CCTCTGCCCT ATGAGGCGCT CACTCAGCTG
ATAGATGAAG CCAGTGAAGG GGATATGAGC ATTTCAATCC TTACACAGGA AATAATGGTA
TATCTAGCCA TGTATATGCG AACCCAGCCT GGCCTCTTTG CTGAAATGTT TCGACTTCGA
ATTGGTCTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCCCTTCG ATGCTCAGCT
GAGGAAGCCA CAGAGGGCCT GATGAATCTC AGTCCTTCGG CCATGAAGAA TCTCCTGCAT
CACATTCTCA GCGGCAAGGA GTTTGGAGTG GAACGAAGCG TTCGTCCCAC TGATTCAAAT
GTCAGTCCTG CTATTTCTAT CCACGAGATT GGTGCTGTTG GAGCAACCAA AACAGAACGA
ACTGGGATCA TGCAGTTAAA AAGTGAGATA AAGCAGGTGG AATTTCGTAG ACTGTCAATC
TCAGCTGAGA GTCAGTCACC TGGAACCTCT ATGACTCCAA GTAGTGGGTC CTTTCCTAGT
GCATATGATC AGCAGTCATC TAAAGATAGT CGTCAAGGTC AATGGCAACG CCGAAGAAGG
CTGGATGGGG CACTGAATAG AGTTCCAGTT GGATTTTATC AGAAAGTATG GAAAGTTTTG
CAGAAGTGTC ACGGACTTTC TGTTGAAGGG TTTGTCCTTC CTTCCTCTAC CACTAGAGAG
ATGACTCCAG GTGAGATTAA ATTCTCTGTT CATGTGGAGT CTGTCCTGAA TCGTGTACCT
CAGCCAGAGT ACCGTCAGCT GCTGGTTGAA GCCATCCTTG TCCTCACCAT GCTGGCAGAT
ATTGAAATTC ATAGCATCGG AAGCATCATT GCTGTGGAAA AAATAGTGCA TATTGCCAAT
GACTTGTTCC TTCAAGAACA GAAAACCCTT GGCGCAGATG ATACCATGTT GGCAAAGGAT
CCCGCATCTG GCATCTGTAC TCTTCTGTAT GACAGTGCAC CCAGTGGCAG GTTTGGCACC
ATGACCTACC TCTCCAAGGC AGCCGCCACC TACGTGCAGG AGTTCCTGCC CCACAGCATC
TGTGCCATGC AATGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006724724.1
CDS144..3638
Misc Feature(1)165..>1460(+)
Misc Feature(2)2376..2732(+)
Translation
Position Chain Variation Link
222 222 a, c dbSNP:386352349
317 317 c, t dbSNP:113140625
531 531 g, t dbSNP:151085366
716 716 c, g dbSNP:200904768
810 810 a, g dbSNP:137852548
1039 1039 a, t dbSNP:137852547
1857 1857 a, g dbSNP:113352627
2400 2400 a, g dbSNP:200422936
2513 2513 c, t dbSNP:267606507
2747 2747 a, g dbSNP:111858819
3300 3300 g, t dbSNP:137852546
3330 3330 c, g dbSNP:202007590
3482 3482 g, t dbSNP:267606506
4263 4263 -, ct dbSNP:60008152
4877 4877 a, g dbSNP:199689345
4957 4957 c, t dbSNP:113496726

Target ORF information:

RefSeq Version XM_006724661
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006724661

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGAGCC GGAGTAACTC CGGGGTCCGG CTGGACGGCT ACGCTCGACT GGTGCAACAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACT GGCTTGCTTC CAGCCAGCTA TGATCAGAAA
GATGCTTGGG TCCGAGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
TATCGGAAGA ATGCAGACCG GGATGAGGAT AAGGCAAAGG CCTATGAATT GGAGCAGAGT
GTAGTGAAGC TGATGAGAGG ACTACTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
TCCTTCAAAT ATAGTCAGAG TACTAAGGAT AGCCTCCATG CAAAGTACAA CACCAAAACC
TGTGCCACTG TAGTGGGTGA TGATCAATGG GGACACCTGC AGTTGGATGC TACCTCTGTG
TACCTGCTCT TCTTAGCCCA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAT
GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
TTCGGGATAT GGGAACGTGG AGACAAGACC AACCAAGGGA TCTCAGAGTT GAATGCCAGT
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGATCT GTTTGGTGTG
AAAGGTGGGC CTCAATCAGT TATCCATGTC CTGGCTGATG AAGTACAGCA CTGCCAGTCT
ATCCTAAATT CACTACTGCC CCGTGCTTCA ACATCAAAAG AGGTTGATGC TAGTCTACTC
TCAGTGGTTT CCTTCCCTGC CTTTGCAGTA GAGGATAGCC AGTTGGTGGA GCTCACAAAA
CAGGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGCTA
TTTGAAAACA TTGAGTGTGA ATGGCCATTG TTCTGGACAT ACTTTATTCT TGATGGGGTC
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TATAAAGAGG CTCTTGAAGC AGTCCTCATC
AAGGGCAAAA ATGGAGTCCC ACTTCTGCCA GAGCTGTACA GTGTTCCTCC TGACAGGGTC
GATGAAGAAT ATCAGAATCC TCACACTGTG GACCGAGTCC CCATGGGGAA ATTGCCTCAC
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCCCCT
GGAGAAATTG ATCCCCTGAA TCGCAGGTTT TCTACTGTAC CGAAGCCCGA TGTTGTGGTT
CAAGTCTCCA TTCTAGCTGA AACAGAAGAA ATCAAGACCA TTTTGAAGGA CAAGGGAATT
TACGTGGAGA CCATTGCTGA GGTATACCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC
CACATTTATT CCAGCCTAGG ATGCAACAAT AGAATGAAAC TCAGTGGACG ACCCTACAGA
CACATGGGAG TGCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACTTTC
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTG
GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGCGGATGAC AGGCCAGCCC
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACAAG CTTGAATTCA
AGTATCCTGG CAGCACTCCG AAAAATGCAA GATGGGTATT TTGGTGGGGC AAGGGTTCAA
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACACTTGAG CTTCATGGAC
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG ACAACTATGA TTACCTGGAA
TCTGGCAACT GGATGAATGA TTATGATTCA ACCAGTCATG ATGTTCACAT GTATCTTCCT
ACGAAGTTAT TTCAGGCTTC CCGGCCTTCA TTCAACTTAC TTGATTCACC TCATCCCCGA
CAGGAGAACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CCAGTCTGGG
GAGGTGGACT TTAAAGCACT GGTTTTACAG TTGAAGGAGA CCTCAAGCTT ACAGGAACAA
GCTGATATCC TCTATATGCT GTATACTATG AAAGGACCTG ACTGGAACAC TGAATTGTAT
AATGAACGGA GTGCTACAGT GAGAGAGCTT CTTACCGAGC TGTATGGCAA AGTGGGAGAA
ATTCGTCACT GGGGCCTGAT CCGATACATT TCTGGGATCT TAAGGAAGAA AGTGGAAGCA
CTTGATGAGG CCTGCACAGA CCTTCTCTCC CACCAGAAAC ATTTGACAGT AGGACTTCCT
CCAGAACCTC GAGAAAAGAC TATCTCTGCA CCTCTGCCCT ATGAGGCGCT CACTCAGCTG
ATAGATGAAG CCAGTGAAGG GGATATGAGC ATTTCAATCC TTACACAGGA AATAATGGTA
TATCTAGCCA TGTATATGCG AACCCAGCCT GGCCTCTTTG CTGAAATGTT TCGACTTCGA
ATTGGTCTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCCCTTCG ATGCTCAGCT
GAGGAAGCCA CAGAGGGCCT GATGAATCTC AGTCCTTCGG CCATGAAGAA TCTCCTGCAT
CACATTCTCA GCGGCAAGGA GTTTGGAGTG GAACGAAGCG TTCGTCCCAC TGATTCAAAT
GTCAGTCCTG CTATTTCTAT CCACGAGATT GGTGCTGTTG GAGCAACCAA AACAGAACGA
ACTGGGATCA TGCAGTTAAA AAGTGAGATA AAGCAGGTGG AATTTCGTAG ACTGTCAATC
TCAGCTGAGA GTCAGTCACC TGGAACCTCT ATGACTCCAA GTAGTGGGTC CTTTCCTAGT
GCATATGATC AGCAGTCATC TAAAGATAGT CGTCAAGGTC AATGGCAACG CCGAAGAAGG
CTGGATGGGG CACTGAATAG AGTTCCAGTT GGATTTTATC AGAAAGTATG GAAAGTTTTG
CAGAAGTGTC ACGGACTTTC TGTTGAAGGG TTTGTCCTTC CTTCCTCTAC CACTAGAGAG
ATGACTCCAG GTGAGATTAA ATTCTCTGTT CATGTGGAGT CTGTCCTGAA TCGTGTACCT
CAGCCAGAGT ACCGTCAGCT GCTGGTTGAA GCCATCCTTG TCCTCACCAT GCTGGCAGAT
ATTGAAATTC ATAGCATCGG AAGCATCATT GCTGTGGAAA AAATAGTGCA TATTGCCAAT
GACTTGTTCC TTCAAGAACA GAAAACCCTT GGCGCAGATG ATACCATGTT GGCAAAGGAT
CCCGCATCTG GCATCTGTAC TCTTCTGTAT GACAGTGCAC CCAGTGGCAG GTTTGGCACC
ATGACCTACC TCTCCAAGGC AGCCGCCACC TACGTGCAGG AGTTCCTGCC CCACAGCATC
TGTGCCATGC AATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17843
Accession Version NM_002637.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3672bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002787.1, BC104944.1, X73874.1, BJ998054.1, BX647542.1 and AW450116.1. This sequence is a reference standard in the RefSeqGene project. On Mar 14, 2008 this sequence version replaced gi:141802074. Summary: Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D, also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1.[provided by RefSeq, Feb 2010]. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X73874.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGAGGAGCC GGAGTAACTC CGGGGTCCGG CTGGACGGCT ACGCTCGACT GGTGCAACAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACT GGCTTGCTTC CAGCCAGCTA TGATCAGAAA
GATGCTTGGG TCCGAGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
TATCGGAAGA ATGCAGACCG GGATGAGGAT AAGGCAAAGG CCTATGAATT GGAGCAGAGT
GTAGTGAAGC TGATGAGAGG ACTACTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
TCCTTCAAAT ATAGTCAGAG TACTAAGGAT AGCCTCCATG CAAAGTACAA CACCAAAACC
TGTGCCACTG TAGTGGGTGA TGATCAATGG GGACACCTGC AGTTGGATGC TACCTCTGTG
TACCTGCTCT TCTTAGCCCA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAT
GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
TTCGGGATAT GGGAACGTGG AGACAAGACC AACCAAGGGA TCTCAGAGTT GAATGCCAGT
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGATCT GTTTGGTGTG
AAAGGTGGGC CTCAATCAGT TATCCATGTC CTGGCTGATG AAGTACAGCA CTGCCAGTCT
ATCCTAAATT CACTACTGCC CCGTGCTTCA ACATCAAAAG AGGTTGATGC TAGTCTACTC
TCAGTGGTTT CCTTCCCTGC CTTTGCAGTA GAGGATAGCC AGTTGGTGGA GCTCACAAAA
CAGGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGCTA
TTTGAAAACA TTGAGTGTGA ATGGCCATTG TTCTGGACAT ACTTTATTCT TGATGGGGTC
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TATAAAGAGG CTCTTGAAGC AGTCCTCATC
AAGGGCAAAA ATGGAGTCCC ACTTCTGCCA GAGCTGTACA GTGTTCCTCC TGACAGGGTC
GATGAAGAAT ATCAGAATCC TCACACTGTG GACCGAGTCC CCATGGGGAA ATTGCCTCAC
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCCCCT
GGAGAAATTG ATCCCCTGAA TCGCAGGTTT TCTACTGTAC CGAAGCCCGA TGTTGTGGTT
CAAGTCTCCA TTCTAGCTGA AACAGAAGAA ATCAAGACCA TTTTGAAGGA CAAGGGAATT
TACGTGGAGA CCATTGCTGA GGTATACCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC
CACATTTATT CCAGCCTAGG ATGCAACAAT AGAATGAAAC TCAGTGGACG ACCCTACAGA
CACATGGGAG TGCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACTTTC
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTG
GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGCGGATGAC AGGCCAGCCC
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACAAG CTTGAATTCA
AGTATCCTGG CAGCACTCCG AAAAATGCAA GATGGGTATT TTGGTGGGGC AAGGGTTCAA
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACACTTGAG CTTCATGGAC
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG ACAACTATGA TTACCTGGAA
TCTGGCAACT GGATGAATGA TTATGATTCA ACCAGTCATG CTCGCTGTGG TGATGAAGTT
GCTCGTTATT TAGATCACCT TTTGGCGCAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC
TCACAGAAGG GAGGGCTAGA TCGGTTCCAA GCTGCTGTGC AAACAACCTG CGACTTAATG
TCCTTGGTGA CCAAGGCCAA GGAACTGCAT GTACAGAATG TTCACATGTA TCTTCCTACG
AAGTTATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG ATTCACCTCA TCCCCGACAG
GAGAACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
GTGGACTTTA AAGCACTGGT TTTACAGTTG AAGGAGACCT CAAGCTTACA GGAACAAGCT
GATATCCTCT ATATGCTGTA TACTATGAAA GGACCTGACT GGAACACTGA ATTGTATAAT
GAACGGAGTG CTACAGTGAG AGAGCTTCTT ACCGAGCTGT ATGGCAAAGT GGGAGAAATT
CGTCACTGGG GCCTGATCCG ATACATTTCT GGGATCTTAA GGAAGAAAGT GGAAGCACTT
GATGAGGCCT GCACAGACCT TCTCTCCCAC CAGAAACATT TGACAGTAGG ACTTCCTCCA
GAACCTCGAG AAAAGACTAT CTCTGCACCT CTGCCCTATG AGGCGCTCAC TCAGCTGATA
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATCCTTA CACAGGAAAT AATGGTATAT
CTAGCCATGT ATATGCGAAC CCAGCCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG
GAAGCCACAG AGGGCCTGAT GAATCTCAGT CCTTCGGCCA TGAAGAATCT CCTGCATCAC
ATTCTCAGCG GCAAGGAGTT TGGAGTGGAA CGAAGCGTTC GTCCCACTGA TTCAAATGTC
AGTCCTGCTA TTTCTATCCA CGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGGTGGAAT TTCGTAGACT GTCAATCTCA
GCTGAGAGTC AGTCACCTGG AACCTCTATG ACTCCAAGTA GTGGGTCCTT TCCTAGTGCA
TATGATCAGC AGTCATCTAA AGATAGTCGT CAAGGTCAAT GGCAACGCCG AAGAAGGCTG
GATGGGGCAC TGAATAGAGT TCCAGTTGGA TTTTATCAGA AAGTATGGAA AGTTTTGCAG
AAGTGTCACG GACTTTCTGT TGAAGGGTTT GTCCTTCCTT CCTCTACCAC TAGAGAGATG
ACTCCAGGTG AGATTAAATT CTCTGTTCAT GTGGAGTCTG TCCTGAATCG TGTACCTCAG
CCAGAGTACC GTCAGCTGCT GGTTGAAGCC ATCCTTGTCC TCACCATGCT GGCAGATATT
GAAATTCATA GCATCGGAAG CATCATTGCT GTGGAAAAAA TAGTGCATAT TGCCAATGAC
TTGTTCCTTC AAGAACAGAA AACCCTTGGC GCAGATGATA CCATGTTGGC AAAGGATCCC
GCATCTGGCA TCTGTACTCT TCTGTATGAC AGTGCACCCA GTGGCAGGTT TGGCACCATG
ACCTACCTCT CCAAGGCAGC CGCCACCTAC GTGCAGGAGT TCCTGCCCCA CAGCATCTGT
GCCATGCAAT GA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_002628.2
CDS302..3973
Misc Feature(1)290..292(+)
Misc Feature(2)323..>1618(+)
Misc Feature(3)2504..2506(+)
Misc Feature(4)2573..2575(+)
Misc Feature(5)2711..3067(+)
Misc Feature(6)2729..2821(+)
Misc Feature(7)3215..3217(+)
Misc Feature(8)3254..3256(+)
Misc Feature(9)3437..3559(+)
Misc Feature(10)3959..3961(+)
Exon (1)1..379
Gene:PHKA1
Gene Synonym:
Exon (2)380..538
Gene:PHKA1
Gene Synonym:
Exon (3)539..586
Gene:PHKA1
Gene Synonym:
Exon (4)587..755
Gene:PHKA1
Gene Synonym:
Exon (5)756..838
Gene:PHKA1
Gene Synonym:
Exon (6)839..919
Gene:PHKA1
Gene Synonym:
Exon (7)920..1018
Gene:PHKA1
Gene Synonym:
Exon (8)1019..1165
Gene:PHKA1
Gene Synonym:
Exon (9)1166..1219
Gene:PHKA1
Gene Synonym:
Exon (10)1220..1342
Gene:PHKA1
Gene Synonym:
Exon (11)1343..1438
Gene:PHKA1
Gene Synonym:
Exon (12)1439..1546
Gene:PHKA1
Gene Synonym:
Exon (13)1547..1625
Gene:PHKA1
Gene Synonym:
Exon (14)1626..1760
Gene:PHKA1
Gene Synonym:
Exon (15)1761..1870
Gene:PHKA1
Gene Synonym:
Exon (16)1871..2015
Gene:PHKA1
Gene Synonym:
Exon (17)2016..2094
Gene:PHKA1
Gene Synonym:
Exon (18)2095..2261
Gene:PHKA1
Gene Synonym:
Exon (19)2262..2438
Gene:PHKA1
Gene Synonym:
Exon (20)2439..2530
Gene:PHKA1
Gene Synonym:
Exon (21)2531..2670
Gene:PHKA1
Gene Synonym:
Exon (22)2671..2827
Gene:PHKA1
Gene Synonym:
Exon (23)2828..2907
Gene:PHKA1
Gene Synonym:
Exon (24)2908..2986
Gene:PHKA1
Gene Synonym:
Exon (25)2987..3116
Gene:PHKA1
Gene Synonym:
Exon (26)3117..3218
Gene:PHKA1
Gene Synonym:
Exon (27)3219..3334
Gene:PHKA1
Gene Synonym:
Exon (28)3335..3373
Gene:PHKA1
Gene Synonym:
Exon (29)3374..3544
Gene:PHKA1
Gene Synonym:
Exon (30)3545..3598
Gene:PHKA1
Gene Synonym:
Exon (31)3599..3799
Gene:PHKA1
Gene Synonym:
Exon (32)3800..6161
Gene:PHKA1
Gene Synonym:
Translation
Position Chain Variation Link
380 380 a, c dbSNP:386352349
475 475 c, t dbSNP:113140625
689 689 g, t dbSNP:151085366
874 874 c, g dbSNP:200904768
968 968 a, g dbSNP:137852548
1197 1197 a, t dbSNP:137852547
2015 2015 a, g dbSNP:113352627
2320 2320 c, g dbSNP:201801160
2735 2735 a, g dbSNP:200422936
2848 2848 c, t dbSNP:267606507
3082 3082 a, g dbSNP:111858819
3635 3635 g, t dbSNP:137852546
3665 3665 c, g dbSNP:202007590
3817 3817 g, t dbSNP:267606506
4598 4598 -, ct dbSNP:60008152
5212 5212 a, g dbSNP:199689345
5292 5292 c, t dbSNP:113496726

Target ORF information:

RefSeq Version NM_002637
Organism Homo sapiens (human)
Definition Homo sapiens phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002637

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGAGGAGCC GGAGTAACTC CGGGGTCCGG CTGGACGGCT ACGCTCGACT GGTGCAACAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACT GGCTTGCTTC CAGCCAGCTA TGATCAGAAA
GATGCTTGGG TCCGAGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
TATCGGAAGA ATGCAGACCG GGATGAGGAT AAGGCAAAGG CCTATGAATT GGAGCAGAGT
GTAGTGAAGC TGATGAGAGG ACTACTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
TCCTTCAAAT ATAGTCAGAG TACTAAGGAT AGCCTCCATG CAAAGTACAA CACCAAAACC
TGTGCCACTG TAGTGGGTGA TGATCAATGG GGACACCTGC AGTTGGATGC TACCTCTGTG
TACCTGCTCT TCTTAGCCCA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAT
GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
TTCGGGATAT GGGAACGTGG AGACAAGACC AACCAAGGGA TCTCAGAGTT GAATGCCAGT
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGATCT GTTTGGTGTG
AAAGGTGGGC CTCAATCAGT TATCCATGTC CTGGCTGATG AAGTACAGCA CTGCCAGTCT
ATCCTAAATT CACTACTGCC CCGTGCTTCA ACATCAAAAG AGGTTGATGC TAGTCTACTC
TCAGTGGTTT CCTTCCCTGC CTTTGCAGTA GAGGATAGCC AGTTGGTGGA GCTCACAAAA
CAGGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGCTA
TTTGAAAACA TTGAGTGTGA ATGGCCATTG TTCTGGACAT ACTTTATTCT TGATGGGGTC
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TATAAAGAGG CTCTTGAAGC AGTCCTCATC
AAGGGCAAAA ATGGAGTCCC ACTTCTGCCA GAGCTGTACA GTGTTCCTCC TGACAGGGTC
GATGAAGAAT ATCAGAATCC TCACACTGTG GACCGAGTCC CCATGGGGAA ATTGCCTCAC
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCCCCT
GGAGAAATTG ATCCCCTGAA TCGCAGGTTT TCTACTGTAC CGAAGCCCGA TGTTGTGGTT
CAAGTCTCCA TTCTAGCTGA AACAGAAGAA ATCAAGACCA TTTTGAAGGA CAAGGGAATT
TACGTGGAGA CCATTGCTGA GGTATACCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC
CACATTTATT CCAGCCTAGG ATGCAACAAT AGAATGAAAC TCAGTGGACG ACCCTACAGA
CACATGGGAG TGCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACTTTC
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTG
GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGCGGATGAC AGGCCAGCCC
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACAAG CTTGAATTCA
AGTATCCTGG CAGCACTCCG AAAAATGCAA GATGGGTATT TTGGTGGGGC AAGGGTTCAA
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACACTTGAG CTTCATGGAC
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG ACAACTATGA TTACCTGGAA
TCTGGCAACT GGATGAATGA TTATGATTCA ACCAGTCATG CTCGCTGTGG TGATGAAGTT
GCTCGTTATT TAGATCACCT TTTGGCGCAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC
TCACAGAAGG GAGGGCTAGA TCGGTTCCAA GCTGCTGTGC AAACAACCTG CGACTTAATG
TCCTTGGTGA CCAAGGCCAA GGAACTGCAT GTACAGAATG TTCACATGTA TCTTCCTACG
AAGTTATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG ATTCACCTCA TCCCCGACAG
GAGAACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
GTGGACTTTA AAGCACTGGT TTTACAGTTG AAGGAGACCT CAAGCTTACA GGAACAAGCT
GATATCCTCT ATATGCTGTA TACTATGAAA GGACCTGACT GGAACACTGA ATTGTATAAT
GAACGGAGTG CTACAGTGAG AGAGCTTCTT ACCGAGCTGT ATGGCAAAGT GGGAGAAATT
CGTCACTGGG GCCTGATCCG ATACATTTCT GGGATCTTAA GGAAGAAAGT GGAAGCACTT
GATGAGGCCT GCACAGACCT TCTCTCCCAC CAGAAACATT TGACAGTAGG ACTTCCTCCA
GAACCTCGAG AAAAGACTAT CTCTGCACCT CTGCCCTATG AGGCGCTCAC TCAGCTGATA
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATCCTTA CACAGGAAAT AATGGTATAT
CTAGCCATGT ATATGCGAAC CCAGCCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG
GAAGCCACAG AGGGCCTGAT GAATCTCAGT CCTTCGGCCA TGAAGAATCT CCTGCATCAC
ATTCTCAGCG GCAAGGAGTT TGGAGTGGAA CGAAGCGTTC GTCCCACTGA TTCAAATGTC
AGTCCTGCTA TTTCTATCCA CGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGGTGGAAT TTCGTAGACT GTCAATCTCA
GCTGAGAGTC AGTCACCTGG AACCTCTATG ACTCCAAGTA GTGGGTCCTT TCCTAGTGCA
TATGATCAGC AGTCATCTAA AGATAGTCGT CAAGGTCAAT GGCAACGCCG AAGAAGGCTG
GATGGGGCAC TGAATAGAGT TCCAGTTGGA TTTTATCAGA AAGTATGGAA AGTTTTGCAG
AAGTGTCACG GACTTTCTGT TGAAGGGTTT GTCCTTCCTT CCTCTACCAC TAGAGAGATG
ACTCCAGGTG AGATTAAATT CTCTGTTCAT GTGGAGTCTG TCCTGAATCG TGTACCTCAG
CCAGAGTACC GTCAGCTGCT GGTTGAAGCC ATCCTTGTCC TCACCATGCT GGCAGATATT
GAAATTCATA GCATCGGAAG CATCATTGCT GTGGAAAAAA TAGTGCATAT TGCCAATGAC
TTGTTCCTTC AAGAACAGAA AACCCTTGGC GCAGATGATA CCATGTTGGC AAAGGATCCC
GCATCTGGCA TCTGTACTCT TCTGTATGAC AGTGCACCCA GTGGCAGGTT TGGCACCATG
ACCTACCTCT CCAAGGCAGC CGCCACCTAC GTGCAGGAGT TCCTGCCCCA CAGCATCTGT
GCCATGCAAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16403
Accession Version NM_001122670.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002787.1, BC104944.1, X73874.1, BJ998054.1, BX647542.1 and AW450116.1. Summary: Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D, also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1.[provided by RefSeq, Feb 2010]. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC104944.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGAGGAGCC GGAGTAACTC CGGGGTCCGG CTGGACGGCT ACGCTCGACT GGTGCAACAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACT GGCTTGCTTC CAGCCAGCTA TGATCAGAAA
GATGCTTGGG TCCGAGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
TATCGGAAGA ATGCAGACCG GGATGAGGAT AAGGCAAAGG CCTATGAATT GGAGCAGAGT
GTAGTGAAGC TGATGAGAGG ACTACTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
TCCTTCAAAT ATAGTCAGAG TACTAAGGAT AGCCTCCATG CAAAGTACAA CACCAAAACC
TGTGCCACTG TAGTGGGTGA TGATCAATGG GGACACCTGC AGTTGGATGC TACCTCTGTG
TACCTGCTCT TCTTAGCCCA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAT
GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
TTCGGGATAT GGGAACGTGG AGACAAGACC AACCAAGGGA TCTCAGAGTT GAATGCCAGT
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGATCT GTTTGGTGTG
AAAGGTGGGC CTCAATCAGT TATCCATGTC CTGGCTGATG AAGTACAGCA CTGCCAGTCT
ATCCTAAATT CACTACTGCC CCGTGCTTCA ACATCAAAAG AGGTTGATGC TAGTCTACTC
TCAGTGGTTT CCTTCCCTGC CTTTGCAGTA GAGGATAGCC AGTTGGTGGA GCTCACAAAA
CAGGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGCTA
TTTGAAAACA TTGAGTGTGA ATGGCCATTG TTCTGGACAT ACTTTATTCT TGATGGGGTC
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TATAAAGAGG CTCTTGAAGC AGTCCTCATC
AAGGGCAAAA ATGGAGTCCC ACTTCTGCCA GAGCTGTACA GTGTTCCTCC TGACAGGGTC
GATGAAGAAT ATCAGAATCC TCACACTGTG GACCGAGTCC CCATGGGGAA ATTGCCTCAC
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCCCCT
GGAGAAATTG ATCCCCTGAA TCGCAGGTTT TCTACTGTAC CGAAGCCCGA TGTTGTGGTT
CAAGTCTCCA TTCTAGCTGA AACAGAAGAA ATCAAGACCA TTTTGAAGGA CAAGGGAATT
TACGTGGAGA CCATTGCTGA GGTATACCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC
CACATTTATT CCAGCCTAGG ATGCAACAAT AGAATGAAAC TCAGTGGACG ACCCTACAGA
CACATGGGAG TGCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACTTTC
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTG
GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGCGGATGAC AGGCCAGCCC
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACAAG CTTGAATTCA
AGTATCCTGG CAGCACTCCG AAAAATGCAA GATGGGTATT TTGGTGGGGC AAGGGTTCAA
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACACTTGAG CTTCATGGAC
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG ACAACTATGA TTACCTGGAA
TCTGGCAACT GGATGAATGA TTATGATTCA ACCAGTCATG CTCGCTGTGG TGATGAAGTT
GCTCGTTATT TAGATCACCT TTTGGCGCAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC
TCACAGAAGG GAGGGCTAGA TCGGTTCCAA GCTGCTGTGC AAACAACCTG CGACTTAATG
TCCTTGGTGA CCAAGGCCAA GGAACTGCAT GTACAGAATG TTCACATGTA TCTTCCTACG
AAGTTATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG ATTCACCTCA TCCCCGACAG
GAGAACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
GTGGACTTTA AAGCACTGGT TTTACAGTTG AAGGAGACCT CAAGCTTACA GGAACAAGCT
GATATCCTCT ATATGCTGTA TACTATGAAA GGACCTGACT GGAACACTGA ATTGTATAAT
GAACGGAGTG CTACAGTGAG AGAGCTTCTT ACCGAGCTGT ATGGCAAAGT GGGAGAAATT
CGTCACTGGG GCCTGATCCG ATACATTTCT GGGATCTTAA GGAAGAAAGT GGAAGCACTT
GATGAGGCCT GCACAGACCT TCTCTCCCAC CAGAAACATT TGACAGTAGG ACTTCCTCCA
GAACCTCGAG AAAAGACTAT CTCTGCACCT CTGCCCTATG AGGCGCTCAC TCAGCTGATA
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATCCTTA CACAGGAAAT AATGGTATAT
CTAGCCATGT ATATGCGAAC CCAGCCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG
GAAGCCACAG AGGGCCTGAT GAATCTCAGT CCTTCGGCCA TGAAGAATCT CCTGCATCAC
ATTCTCAGCG GCAAGGAGTT TGGAGTGGAA CGAAGCGTTC GTCCCACTGA TTCAAATGTC
AGTCCTGCTA TTTCTATCCA CGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGTCACCTG GAACCTCTAT GACTCCAAGT
AGTGGGTCCT TTCCTAGTGC ATATGATCAG CAGTCATCTA AAGATAGTCG TCAAGGTCAA
TGGCAACGCC GAAGAAGGCT GGATGGGGCA CTGAATAGAG TTCCAGTTGG ATTTTATCAG
AAAGTATGGA AAGTTTTGCA GAAGTGTCAC GGACTTTCTG TTGAAGGGTT TGTCCTTCCT
TCCTCTACCA CTAGAGAGAT GACTCCAGGT GAGATTAAAT TCTCTGTTCA TGTGGAGTCT
GTCCTGAATC GTGTACCTCA GCCAGAGTAC CGTCAGCTGC TGGTTGAAGC CATCCTTGTC
CTCACCATGC TGGCAGATAT TGAAATTCAT AGCATCGGAA GCATCATTGC TGTGGAAAAA
ATAGTGCATA TTGCCAATGA CTTGTTCCTT CAAGAACAGA AAACCCTTGG CGCAGATGAT
ACCATGTTGG CAAAGGATCC CGCATCTGGC ATCTGTACTC TTCTGTATGA CAGTGCACCC
AGTGGCAGGT TTGGCACCAT GACCTACCTC TCCAAGGCAG CCGCCACCTA CGTGCAGGAG
TTCCTGCCCC ACAGCATCTG TGCCATGCAA TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001116142.1
CDS302..3934
Misc Feature(1)290..292(+)
Misc Feature(2)323..>1618(+)
Misc Feature(3)2504..2506(+)
Misc Feature(4)2573..2575(+)
Misc Feature(5)2711..3067(+)
Misc Feature(6)2729..2821(+)
Misc Feature(7)3215..3217(+)
Misc Feature(8)3254..3256(+)
Misc Feature(9)3398..3520(+)
Misc Feature(10)3920..3922(+)
Exon (1)1..379
Gene:PHKA1
Gene Synonym:
Exon (2)380..538
Gene:PHKA1
Gene Synonym:
Exon (3)539..586
Gene:PHKA1
Gene Synonym:
Exon (4)587..755
Gene:PHKA1
Gene Synonym:
Exon (5)756..838
Gene:PHKA1
Gene Synonym:
Exon (6)839..919
Gene:PHKA1
Gene Synonym:
Exon (7)920..1018
Gene:PHKA1
Gene Synonym:
Exon (8)1019..1165
Gene:PHKA1
Gene Synonym:
Exon (9)1166..1219
Gene:PHKA1
Gene Synonym:
Exon (10)1220..1342
Gene:PHKA1
Gene Synonym:
Exon (11)1343..1438
Gene:PHKA1
Gene Synonym:
Exon (12)1439..1546
Gene:PHKA1
Gene Synonym:
Exon (13)1547..1625
Gene:PHKA1
Gene Synonym:
Exon (14)1626..1760
Gene:PHKA1
Gene Synonym:
Exon (15)1761..1870
Gene:PHKA1
Gene Synonym:
Exon (16)1871..2015
Gene:PHKA1
Gene Synonym:
Exon (17)2016..2094
Gene:PHKA1
Gene Synonym:
Exon (18)2095..2261
Gene:PHKA1
Gene Synonym:
Exon (19)2262..2438
Gene:PHKA1
Gene Synonym:
Exon (20)2439..2530
Gene:PHKA1
Gene Synonym:
Exon (21)2531..2670
Gene:PHKA1
Gene Synonym:
Exon (22)2671..2827
Gene:PHKA1
Gene Synonym:
Exon (23)2828..2907
Gene:PHKA1
Gene Synonym:
Exon (24)2908..2986
Gene:PHKA1
Gene Synonym:
Exon (25)2987..3116
Gene:PHKA1
Gene Synonym:
Exon (26)3117..3218
Gene:PHKA1
Gene Synonym:
Exon (27)3219..3334
Gene:PHKA1
Gene Synonym:
Exon (28)3335..3505
Gene:PHKA1
Gene Synonym:
Exon (29)3506..3559
Gene:PHKA1
Gene Synonym:
Exon (30)3560..3760
Gene:PHKA1
Gene Synonym:
Exon (31)3761..6122
Gene:PHKA1
Gene Synonym:
Translation
Position Chain Variation Link
380 380 a, c dbSNP:386352349
475 475 c, t dbSNP:113140625
689 689 g, t dbSNP:151085366
874 874 c, g dbSNP:200904768
968 968 a, g dbSNP:137852548
1197 1197 a, t dbSNP:137852547
2015 2015 a, g dbSNP:113352627
2320 2320 c, g dbSNP:201801160
2735 2735 a, g dbSNP:200422936
2848 2848 c, t dbSNP:267606507
3082 3082 a, g dbSNP:111858819
3596 3596 g, t dbSNP:137852546
3626 3626 c, g dbSNP:202007590
3778 3778 g, t dbSNP:267606506
4559 4559 -, ct dbSNP:60008152
5173 5173 a, g dbSNP:199689345
5253 5253 c, t dbSNP:113496726

Target ORF information:

RefSeq Version NM_001122670
Organism Homo sapiens (human)
Definition Homo sapiens phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001122670

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGAGGAGCC GGAGTAACTC CGGGGTCCGG CTGGACGGCT ACGCTCGACT GGTGCAACAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACT GGCTTGCTTC CAGCCAGCTA TGATCAGAAA
GATGCTTGGG TCCGAGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
TATCGGAAGA ATGCAGACCG GGATGAGGAT AAGGCAAAGG CCTATGAATT GGAGCAGAGT
GTAGTGAAGC TGATGAGAGG ACTACTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
TCCTTCAAAT ATAGTCAGAG TACTAAGGAT AGCCTCCATG CAAAGTACAA CACCAAAACC
TGTGCCACTG TAGTGGGTGA TGATCAATGG GGACACCTGC AGTTGGATGC TACCTCTGTG
TACCTGCTCT TCTTAGCCCA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAT
GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
TTCGGGATAT GGGAACGTGG AGACAAGACC AACCAAGGGA TCTCAGAGTT GAATGCCAGT
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGATCT GTTTGGTGTG
AAAGGTGGGC CTCAATCAGT TATCCATGTC CTGGCTGATG AAGTACAGCA CTGCCAGTCT
ATCCTAAATT CACTACTGCC CCGTGCTTCA ACATCAAAAG AGGTTGATGC TAGTCTACTC
TCAGTGGTTT CCTTCCCTGC CTTTGCAGTA GAGGATAGCC AGTTGGTGGA GCTCACAAAA
CAGGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGCTA
TTTGAAAACA TTGAGTGTGA ATGGCCATTG TTCTGGACAT ACTTTATTCT TGATGGGGTC
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TATAAAGAGG CTCTTGAAGC AGTCCTCATC
AAGGGCAAAA ATGGAGTCCC ACTTCTGCCA GAGCTGTACA GTGTTCCTCC TGACAGGGTC
GATGAAGAAT ATCAGAATCC TCACACTGTG GACCGAGTCC CCATGGGGAA ATTGCCTCAC
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCCCCT
GGAGAAATTG ATCCCCTGAA TCGCAGGTTT TCTACTGTAC CGAAGCCCGA TGTTGTGGTT
CAAGTCTCCA TTCTAGCTGA AACAGAAGAA ATCAAGACCA TTTTGAAGGA CAAGGGAATT
TACGTGGAGA CCATTGCTGA GGTATACCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC
CACATTTATT CCAGCCTAGG ATGCAACAAT AGAATGAAAC TCAGTGGACG ACCCTACAGA
CACATGGGAG TGCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACTTTC
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTG
GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGCGGATGAC AGGCCAGCCC
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACAAG CTTGAATTCA
AGTATCCTGG CAGCACTCCG AAAAATGCAA GATGGGTATT TTGGTGGGGC AAGGGTTCAA
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACACTTGAG CTTCATGGAC
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG ACAACTATGA TTACCTGGAA
TCTGGCAACT GGATGAATGA TTATGATTCA ACCAGTCATG CTCGCTGTGG TGATGAAGTT
GCTCGTTATT TAGATCACCT TTTGGCGCAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC
TCACAGAAGG GAGGGCTAGA TCGGTTCCAA GCTGCTGTGC AAACAACCTG CGACTTAATG
TCCTTGGTGA CCAAGGCCAA GGAACTGCAT GTACAGAATG TTCACATGTA TCTTCCTACG
AAGTTATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG ATTCACCTCA TCCCCGACAG
GAGAACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
GTGGACTTTA AAGCACTGGT TTTACAGTTG AAGGAGACCT CAAGCTTACA GGAACAAGCT
GATATCCTCT ATATGCTGTA TACTATGAAA GGACCTGACT GGAACACTGA ATTGTATAAT
GAACGGAGTG CTACAGTGAG AGAGCTTCTT ACCGAGCTGT ATGGCAAAGT GGGAGAAATT
CGTCACTGGG GCCTGATCCG ATACATTTCT GGGATCTTAA GGAAGAAAGT GGAAGCACTT
GATGAGGCCT GCACAGACCT TCTCTCCCAC CAGAAACATT TGACAGTAGG ACTTCCTCCA
GAACCTCGAG AAAAGACTAT CTCTGCACCT CTGCCCTATG AGGCGCTCAC TCAGCTGATA
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATCCTTA CACAGGAAAT AATGGTATAT
CTAGCCATGT ATATGCGAAC CCAGCCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG
GAAGCCACAG AGGGCCTGAT GAATCTCAGT CCTTCGGCCA TGAAGAATCT CCTGCATCAC
ATTCTCAGCG GCAAGGAGTT TGGAGTGGAA CGAAGCGTTC GTCCCACTGA TTCAAATGTC
AGTCCTGCTA TTTCTATCCA CGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGTCACCTG GAACCTCTAT GACTCCAAGT
AGTGGGTCCT TTCCTAGTGC ATATGATCAG CAGTCATCTA AAGATAGTCG TCAAGGTCAA
TGGCAACGCC GAAGAAGGCT GGATGGGGCA CTGAATAGAG TTCCAGTTGG ATTTTATCAG
AAAGTATGGA AAGTTTTGCA GAAGTGTCAC GGACTTTCTG TTGAAGGGTT TGTCCTTCCT
TCCTCTACCA CTAGAGAGAT GACTCCAGGT GAGATTAAAT TCTCTGTTCA TGTGGAGTCT
GTCCTGAATC GTGTACCTCA GCCAGAGTAC CGTCAGCTGC TGGTTGAAGC CATCCTTGTC
CTCACCATGC TGGCAGATAT TGAAATTCAT AGCATCGGAA GCATCATTGC TGTGGAAAAA
ATAGTGCATA TTGCCAATGA CTTGTTCCTT CAAGAACAGA AAACCCTTGG CGCAGATGAT
ACCATGTTGG CAAAGGATCC CGCATCTGGC ATCTGTACTC TTCTGTATGA CAGTGCACCC
AGTGGCAGGT TTGGCACCAT GACCTACCTC TCCAAGGCAG CCGCCACCTA CGTGCAGGAG
TTCCTGCCCC ACAGCATCTG TGCCATGCAA TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16802
Accession Version NM_001172436.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3456bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002787.1, BC143501.1, DA171597.1, DA998603.1, BX647542.1 and AW450116.1. Summary: Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D, also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1.[provided by RefSeq, Feb 2010]. Transcript Variant: This variant (3) lacks two alternate in-frame exons in the middle and 3' coding regions, respectively, compared to variant 1, resulting in a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143501.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGAGGAGCC GGAGTAACTC CGGGGTCCGG CTGGACGGCT ACGCTCGACT GGTGCAACAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACT GGCTTGCTTC CAGCCAGCTA TGATCAGAAA
GATGCTTGGG TCCGAGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
TATCGGAAGA ATGCAGACCG GGATGAGGAT AAGGCAAAGG CCTATGAATT GGAGCAGAGT
GTAGTGAAGC TGATGAGAGG ACTACTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
TCCTTCAAAT ATAGTCAGAG TACTAAGGAT AGCCTCCATG CAAAGTACAA CACCAAAACC
TGTGCCACTG TAGTGGGTGA TGATCAATGG GGACACCTGC AGTTGGATGC TACCTCTGTG
TACCTGCTCT TCTTAGCCCA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAT
GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
TTCGGGATAT GGGAACGTGG AGACAAGACC AACCAAGGGA TCTCAGAGTT GAATGCCAGT
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGATCT GTTTGGTGTG
AAAGGTGGGC CTCAATCAGT TATCCATGTC CTGGCTGATG AAGTACAGCA CTGCCAGTCT
ATCCTAAATT CACTACTGCC CCGTGCTTCA ACATCAAAAG AGGTTGATGC TAGTCTACTC
TCAGTGGTTT CCTTCCCTGC CTTTGCAGTA GAGGATAGCC AGTTGGTGGA GCTCACAAAA
CAGGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGCTA
TTTGAAAACA TTGAGTGTGA ATGGCCATTG TTCTGGACAT ACTTTATTCT TGATGGGGTC
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TATAAAGAGG CTCTTGAAGC AGTCCTCATC
AAGGGCAAAA ATGGAGTCCC ACTTCTGCCA GAGCTGTACA GTGTTCCTCC TGACAGGGTC
GATGAAGAAT ATCAGAATCC TCACACTGTG GACCGAGTCC CCATGGGGAA ATTGCCTCAC
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCCCCT
GGAGAAATTG ATCCCCTGAA TCGCAGGTTT TCTACTGTAC CGAAGCCCGA TGTTGTGGTT
CAAGTCTCCA TTCTAGCTGA AACAGAAGAA ATCAAGACCA TTTTGAAGGA CAAGGGAATT
TACGTGGAGA CCATTGCTGA GGTATACCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC
CACATTTATT CCAGCCTAGG ATGCAACAAT AGAATGAAAC TCAGTGGACG ACCCTACAGA
CACATGGGAG TGCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACTTTC
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTG
GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGCGGATGAC AGGCCAGCCC
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACAAG CTTGAATTCA
AGTATCCTGG CAGCACTCCG AAAAATGCAA GATGGGTATT TTGGTGGGGC AAGGGTTCAA
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACACTTGAG CTTCATGGAC
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG ACAACTATGA TTACCTGGAA
TCTGGCAACT GGATGAATGA TTATGATTCA ACCAGTCATG ATGTTCACAT GTATCTTCCT
ACGAAGTTAT TTCAGGCTTC CCGGCCTTCA TTCAACTTAC TTGATTCACC TCATCCCCGA
CAGGAGAACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CCAGTCTGGG
GAGGTGGACT TTAAAGCACT GGTTTTACAG TTGAAGGAGA CCTCAAGCTT ACAGGAACAA
GCTGATATCC TCTATATGCT GTATACTATG AAAGGACCTG ACTGGAACAC TGAATTGTAT
AATGAACGGA GTGCTACAGT GAGAGAGCTT CTTACCGAGC TGTATGGCAA AGTGGGAGAA
ATTCGTCACT GGGGCCTGAT CCGATACATT TCTGGGATCT TAAGGAAGAA AGTGGAAGCA
CTTGATGAGG CCTGCACAGA CCTTCTCTCC CACCAGAAAC ATTTGACAGT AGGACTTCCT
CCAGAACCTC GAGAAAAGAC TATCTCTGCA CCTCTGCCCT ATGAGGCGCT CACTCAGCTG
ATAGATGAAG CCAGTGAAGG GGATATGAGC ATTTCAATCC TTACACAGGA AATAATGGTA
TATCTAGCCA TGTATATGCG AACCCAGCCT GGCCTCTTTG CTGAAATGTT TCGACTTCGA
ATTGGTCTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCCCTTCG ATGCTCAGCT
GAGGAAGCCA CAGAGGGCCT GATGAATCTC AGTCCTTCGG CCATGAAGAA TCTCCTGCAT
CACATTCTCA GCGGCAAGGA GTTTGGAGTG GAACGAAGCG TTCGTCCCAC TGATTCAAAT
GTCAGTCCTG CTATTTCTAT CCACGAGATT GGTGCTGTTG GAGCAACCAA AACAGAACGA
ACTGGGATCA TGCAGTTAAA AAGTGAGATA AAGCAGTCAC CTGGAACCTC TATGACTCCA
AGTAGTGGGT CCTTTCCTAG TGCATATGAT CAGCAGTCAT CTAAAGATAG TCGTCAAGGT
CAATGGCAAC GCCGAAGAAG GCTGGATGGG GCACTGAATA GAGTTCCAGT TGGATTTTAT
CAGAAAGTAT GGAAAGTTTT GCAGAAGTGT CACGGACTTT CTGTTGAAGG GTTTGTCCTT
CCTTCCTCTA CCACTAGAGA GATGACTCCA GGTGAGATTA AATTCTCTGT TCATGTGGAG
TCTGTCCTGA ATCGTGTACC TCAGCCAGAG TACCGTCAGC TGCTGGTTGA AGCCATCCTT
GTCCTCACCA TGCTGGCAGA TATTGAAATT CATAGCATCG GAAGCATCAT TGCTGTGGAA
AAAATAGTGC ATATTGCCAA TGACTTGTTC CTTCAAGAAC AGAAAACCCT TGGCGCAGAT
GATACCATGT TGGCAAAGGA TCCCGCATCT GGCATCTGTA CTCTTCTGTA TGACAGTGCA
CCCAGTGGCA GGTTTGGCAC CATGACCTAC CTCTCCAAGG CAGCCGCCAC CTACGTGCAG
GAGTTCCTGC CCCACAGCAT CTGTGCCATG CAATGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001165907.1
CDS302..3757
Misc Feature(1)290..292(+)
Misc Feature(2)323..>1618(+)
Misc Feature(3)2534..2890(+)
Exon (1)1..379
Gene:PHKA1
Gene Synonym:
Exon (2)380..538
Gene:PHKA1
Gene Synonym:
Exon (3)539..586
Gene:PHKA1
Gene Synonym:
Exon (4)587..755
Gene:PHKA1
Gene Synonym:
Exon (5)756..838
Gene:PHKA1
Gene Synonym:
Exon (6)839..919
Gene:PHKA1
Gene Synonym:
Exon (7)920..1018
Gene:PHKA1
Gene Synonym:
Exon (8)1019..1165
Gene:PHKA1
Gene Synonym:
Exon (9)1166..1219
Gene:PHKA1
Gene Synonym:
Exon (10)1220..1342
Gene:PHKA1
Gene Synonym:
Exon (11)1343..1438
Gene:PHKA1
Gene Synonym:
Exon (12)1439..1546
Gene:PHKA1
Gene Synonym:
Exon (13)1547..1625
Gene:PHKA1
Gene Synonym:
Exon (14)1626..1760
Gene:PHKA1
Gene Synonym:
Exon (15)1761..1870
Gene:PHKA1
Gene Synonym:
Exon (16)1871..2015
Gene:PHKA1
Gene Synonym:
Exon (17)2016..2094
Gene:PHKA1
Gene Synonym:
Exon (18)2095..2261
Gene:PHKA1
Gene Synonym:
Exon (19)2262..2353
Gene:PHKA1
Gene Synonym:
Exon (20)2354..2493
Gene:PHKA1
Gene Synonym:
Exon (21)2494..2650
Gene:PHKA1
Gene Synonym:
Exon (22)2651..2730
Gene:PHKA1
Gene Synonym:
Exon (23)2731..2809
Gene:PHKA1
Gene Synonym:
Exon (24)2810..2939
Gene:PHKA1
Gene Synonym:
Exon (25)2940..3041
Gene:PHKA1
Gene Synonym:
Exon (26)3042..3157
Gene:PHKA1
Gene Synonym:
Exon (27)3158..3328
Gene:PHKA1
Gene Synonym:
Exon (28)3329..3382
Gene:PHKA1
Gene Synonym:
Exon (29)3383..3583
Gene:PHKA1
Gene Synonym:
Exon (30)3584..5945
Gene:PHKA1
Gene Synonym:
Translation
Position Chain Variation Link
380 380 a, c dbSNP:386352349
475 475 c, t dbSNP:113140625
689 689 g, t dbSNP:151085366
874 874 c, g dbSNP:200904768
968 968 a, g dbSNP:137852548
1197 1197 a, t dbSNP:137852547
2015 2015 a, g dbSNP:113352627
2558 2558 a, g dbSNP:200422936
2671 2671 c, t dbSNP:267606507
2905 2905 a, g dbSNP:111858819
3419 3419 g, t dbSNP:137852546
3449 3449 c, g dbSNP:202007590
3601 3601 g, t dbSNP:267606506
4382 4382 -, ct dbSNP:60008152
4996 4996 a, g dbSNP:199689345
5076 5076 c, t dbSNP:113496726

Target ORF information:

RefSeq Version NM_001172436
Organism Homo sapiens (human)
Definition Homo sapiens phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001172436

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGAGGAGCC GGAGTAACTC CGGGGTCCGG CTGGACGGCT ACGCTCGACT GGTGCAACAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACT GGCTTGCTTC CAGCCAGCTA TGATCAGAAA
GATGCTTGGG TCCGAGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
TATCGGAAGA ATGCAGACCG GGATGAGGAT AAGGCAAAGG CCTATGAATT GGAGCAGAGT
GTAGTGAAGC TGATGAGAGG ACTACTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
TCCTTCAAAT ATAGTCAGAG TACTAAGGAT AGCCTCCATG CAAAGTACAA CACCAAAACC
TGTGCCACTG TAGTGGGTGA TGATCAATGG GGACACCTGC AGTTGGATGC TACCTCTGTG
TACCTGCTCT TCTTAGCCCA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAT
GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
TTCGGGATAT GGGAACGTGG AGACAAGACC AACCAAGGGA TCTCAGAGTT GAATGCCAGT
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGATCT GTTTGGTGTG
AAAGGTGGGC CTCAATCAGT TATCCATGTC CTGGCTGATG AAGTACAGCA CTGCCAGTCT
ATCCTAAATT CACTACTGCC CCGTGCTTCA ACATCAAAAG AGGTTGATGC TAGTCTACTC
TCAGTGGTTT CCTTCCCTGC CTTTGCAGTA GAGGATAGCC AGTTGGTGGA GCTCACAAAA
CAGGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGCTA
TTTGAAAACA TTGAGTGTGA ATGGCCATTG TTCTGGACAT ACTTTATTCT TGATGGGGTC
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TATAAAGAGG CTCTTGAAGC AGTCCTCATC
AAGGGCAAAA ATGGAGTCCC ACTTCTGCCA GAGCTGTACA GTGTTCCTCC TGACAGGGTC
GATGAAGAAT ATCAGAATCC TCACACTGTG GACCGAGTCC CCATGGGGAA ATTGCCTCAC
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCCCCT
GGAGAAATTG ATCCCCTGAA TCGCAGGTTT TCTACTGTAC CGAAGCCCGA TGTTGTGGTT
CAAGTCTCCA TTCTAGCTGA AACAGAAGAA ATCAAGACCA TTTTGAAGGA CAAGGGAATT
TACGTGGAGA CCATTGCTGA GGTATACCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC
CACATTTATT CCAGCCTAGG ATGCAACAAT AGAATGAAAC TCAGTGGACG ACCCTACAGA
CACATGGGAG TGCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACTTTC
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTG
GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGCGGATGAC AGGCCAGCCC
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACAAG CTTGAATTCA
AGTATCCTGG CAGCACTCCG AAAAATGCAA GATGGGTATT TTGGTGGGGC AAGGGTTCAA
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACACTTGAG CTTCATGGAC
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG ACAACTATGA TTACCTGGAA
TCTGGCAACT GGATGAATGA TTATGATTCA ACCAGTCATG ATGTTCACAT GTATCTTCCT
ACGAAGTTAT TTCAGGCTTC CCGGCCTTCA TTCAACTTAC TTGATTCACC TCATCCCCGA
CAGGAGAACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CCAGTCTGGG
GAGGTGGACT TTAAAGCACT GGTTTTACAG TTGAAGGAGA CCTCAAGCTT ACAGGAACAA
GCTGATATCC TCTATATGCT GTATACTATG AAAGGACCTG ACTGGAACAC TGAATTGTAT
AATGAACGGA GTGCTACAGT GAGAGAGCTT CTTACCGAGC TGTATGGCAA AGTGGGAGAA
ATTCGTCACT GGGGCCTGAT CCGATACATT TCTGGGATCT TAAGGAAGAA AGTGGAAGCA
CTTGATGAGG CCTGCACAGA CCTTCTCTCC CACCAGAAAC ATTTGACAGT AGGACTTCCT
CCAGAACCTC GAGAAAAGAC TATCTCTGCA CCTCTGCCCT ATGAGGCGCT CACTCAGCTG
ATAGATGAAG CCAGTGAAGG GGATATGAGC ATTTCAATCC TTACACAGGA AATAATGGTA
TATCTAGCCA TGTATATGCG AACCCAGCCT GGCCTCTTTG CTGAAATGTT TCGACTTCGA
ATTGGTCTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCCCTTCG ATGCTCAGCT
GAGGAAGCCA CAGAGGGCCT GATGAATCTC AGTCCTTCGG CCATGAAGAA TCTCCTGCAT
CACATTCTCA GCGGCAAGGA GTTTGGAGTG GAACGAAGCG TTCGTCCCAC TGATTCAAAT
GTCAGTCCTG CTATTTCTAT CCACGAGATT GGTGCTGTTG GAGCAACCAA AACAGAACGA
ACTGGGATCA TGCAGTTAAA AAGTGAGATA AAGCAGTCAC CTGGAACCTC TATGACTCCA
AGTAGTGGGT CCTTTCCTAG TGCATATGAT CAGCAGTCAT CTAAAGATAG TCGTCAAGGT
CAATGGCAAC GCCGAAGAAG GCTGGATGGG GCACTGAATA GAGTTCCAGT TGGATTTTAT
CAGAAAGTAT GGAAAGTTTT GCAGAAGTGT CACGGACTTT CTGTTGAAGG GTTTGTCCTT
CCTTCCTCTA CCACTAGAGA GATGACTCCA GGTGAGATTA AATTCTCTGT TCATGTGGAG
TCTGTCCTGA ATCGTGTACC TCAGCCAGAG TACCGTCAGC TGCTGGTTGA AGCCATCCTT
GTCCTCACCA TGCTGGCAGA TATTGAAATT CATAGCATCG GAAGCATCAT TGCTGTGGAA
AAAATAGTGC ATATTGCCAA TGACTTGTTC CTTCAAGAAC AGAAAACCCT TGGCGCAGAT
GATACCATGT TGGCAAAGGA TCCCGCATCT GGCATCTGTA CTCTTCTGTA TGACAGTGCA
CCCAGTGGCA GGTTTGGCAC CATGACCTAC CTCTCCAAGG CAGCCGCCAC CTACGTGCAG
GAGTTCCTGC CCCACAGCAT CTGTGCCATG CAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.