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PHKG2 phosphorylase kinase, gamma 2 (testis) [Homo sapiens (human)]

Gene Symbol PHKG2
Entrez Gene ID 5261
Full Name phosphorylase kinase, gamma 2 (testis)
Synonyms GSD9C
General protein information
Preferred Names
phosphorylase b kinase gamma catalytic chain, liver/testis isoform
Names
phosphorylase b kinase gamma catalytic chain, liver/testis isoform
PSK-C3
PHK-gamma-T
PHK-gamma-LT
phosphorylase kinase subunit gamma-2
serine/threonine-protein kinase PHKG2
Phosphorylase kinase, gamma 2 (testis/liver)
phosphorylase b kinase gamma catalytic chain, testis/liver isoform
NP_000285.1
NP_001165903.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

16

16p11.2

Summary Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9C, also known as autosomal liver glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]. lac of sum
Disorder MIM:

172471

Disorder Html: Glycogen storage disease IXc, 613027 (3); Cirrhosis due to liver





The following PHKG2 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHKG2 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu21036 NM_001172432 Homo sapiens phosphorylase kinase, gamma 2 (testis) (PHKG2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu23652 NM_000294 Homo sapiens phosphorylase kinase, gamma 2 (testis) (PHKG2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector in pcDNA3.1+/C-(K)DYK $319

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu21036
Accession Version NM_001172432.1
Sequence Information ORF Nucleotide Sequence (Length: 1125bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product phosphorylase b kinase gamma catalytic chain, liver/testis isoform isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC106886.3, AK293551.1 and CA449954.1. Summary: Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9C, also known as autosomal liver glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]. Transcript Variant: This variant (2) lacks an internal segment in the 3' region, as compared to variant 1. The resulting isoform (2) is shorter and has a different C-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293551.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGACGCTGG ACGTGGGGCC GGAGGATGAG CTGCCCGACT GGGCCGCCGC CAAAGAGTTT 
TACCAGAAGT ACGACCCTAA GGACGTCATC GGCAGAGGAG TGAGCTCTGT GGTCCGCCGT
TGTGTTCATC GAGCTACTGG CCACGAGTTT GCGGTGAAGA TTATGGAAGT GACAGCTGAG
CGGCTGAGTC CTGAGCAGCT GGAGGAGGTG CGGGAAGCCA CACGGCGAGA GACACACATC
CTTCGCCAGG TCGCCGGCCA CCCCCACATC ATCACCCTCA TCGATTCCTA CGAGTCTTCT
AGCTTCATGT TCCTGGTGTT TGACCTGATG CGGAAGGGAG AGCTGTTTGA CTATCTCACA
GAGAAGGTGG CCCTCTCTGA AAAGGAAACC AGGTCCATCA TGCGGTCTCT GCTGGAAGCA
GTGAGCTTTC TCCATGCCAA CAACATTGTG CATCGAGATC TGAAGCCCGA GAATATTCTC
CTAGATGACA ATATGCAGAT CCGACTTTCA GATTTCGGGT TCTCCTGCCA CTTGGAACCT
GGCGAGAAGC TTCGAGAGTT GTGTGGGACC CCAGGGTATC TAGCGCCAGA GATCCTTAAA
TGCTCCATGG ATGAAACCCA CCCAGGCTAT GGCAAGGAGG TCGACCTCTG GGCCTGTGGG
GTGATCTTGT TCACACTCCT GGCTGGCTCG CCACCCTTCT GGCACCGGCG GCAGATCCTG
ATGTTACGCA TGATCATGGA GGGCCAGTAC CAGTTCAGTT CCCCCGAGTG GGATGACCGT
TCCAGCACTG TCAAAGACCT GATCTCCAGG CTGCTGCAGG TGGATCCTGA GGCACGCCTG
ACAGCTGAGC AGGCCCTACA GCACCCCTTC TTTGAGCGTT GTGAAGGCAG CCAACCCTGG
AACCTCACCC CCCGCCAGCG GTTCCGGGTG GCAGTGTGGA CAGTGCTGGC TGCTGGACGA
GTGGCCCTAA GCACCCATCG TGTACGGCCA CTGACCAAGA ATGCACTGTT GAGGGACCCT
TATGCGCTGC GGTCAGTGCG GCACCTCATC GACAACTGTG CCTTCCGGCT CTACGGGCAC
TGGATAAGGA AGCAGTGGAT TGGAAAGCTG ATGGCTTGTG TATGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001165903.1
CDS211..1335
Misc Feature(1)247..1083(+)
Misc Feature(2)280..1083(+)
Misc Feature(3)298..792(+)
Misc Feature(4)298..723(+)
Misc Feature(5)310..792(+)
Misc Feature(6)718..786(+)
Misc Feature(7)1126..1200(+)
Exon (1)1..192
Gene:PHKG2
Gene Synonym:
Exon (2)193..305
Gene:PHKG2
Gene Synonym:
Exon (3)306..481
Gene:PHKG2
Gene Synonym:
Exon (4)482..536
Gene:PHKG2
Gene Synonym:
Exon (5)537..602
Gene:PHKG2
Gene Synonym:
Exon (6)603..766
Gene:PHKG2
Gene Synonym:
Exon (7)767..857
Gene:PHKG2
Gene Synonym:
Exon (8)858..1011
Gene:PHKG2
Gene Synonym:
Exon (9)1012..1137
Gene:PHKG2
Gene Synonym:
Exon (10)1138..1293
Gene:PHKG2
Gene Synonym:
Exon (11)1294..2351
Gene:PHKG2
Gene Synonym:
Translation
Position Chain Variation Link
65 65 c, t dbSNP:536542470
97 97 c, t dbSNP:11863277
102 102 a, c dbSNP:566930196
151 151 c, g dbSNP:746968932
160 160 c, g dbSNP:112201430
183 183 c, g, t dbSNP:556945561
188 188 c, t dbSNP:11547255
190 190 c, g dbSNP:534374752
193 193 -, c dbSNP:754399010
197 197 c, g dbSNP:11547256
219 219 a, g dbSNP:780095616
222 222 c, t dbSNP:565389823
231 231 a, c, g dbSNP:200427925
232 232 g, t dbSNP:375063162
258 258 a, c dbSNP:747851738
278 278 a, c dbSNP:769648334
293 293 a, t dbSNP:777228104
301 301 a, g dbSNP:748940118
326 326 a, g dbSNP:373732367
329 329 a, g dbSNP:756077227
334 334 a, g dbSNP:777606340
337 337 c, t dbSNP:202177461
340 340 c, t dbSNP:137853590
341 341 a, g dbSNP:778737777
349 349 a, g dbSNP:373915359
352 352 c, t dbSNP:202145162
354 354 c, t dbSNP:141595425
355 355 a, g dbSNP:776935753
357 357 a, g dbSNP:527291776
362 362 c, t dbSNP:770253840
363 363 a, g dbSNP:368095671
366 366 a, g dbSNP:763340630
372 372 g, t dbSNP:199854449
379 379 g, t dbSNP:766541878
384 384 a, t dbSNP:56207641
385 385 a, g dbSNP:144930894
391 391 c, t dbSNP:201199884
392 392 a, g dbSNP:147950160
402 402 c, t dbSNP:372292588
421 421 c, t dbSNP:756052327
422 422 a, g dbSNP:777894749
424 424 a, g dbSNP:753644625
434 434 a, g dbSNP:538127151
437 437 a, g dbSNP:138299462
443 443 c, g dbSNP:745537506
445 445 c, t dbSNP:758053261
454 454 c, t dbSNP:779594016
455 455 a, g dbSNP:746541930
462 462 c, t dbSNP:568221601
463 463 a, g dbSNP:535265672
465 465 c, t dbSNP:775095281
466 466 a, g dbSNP:143983247
470 470 -, c dbSNP:764699954
474 474 c, g dbSNP:774768035
477 477 c, t dbSNP:759795602
498 498 c, t dbSNP:56029513
500 500 a, g dbSNP:757735184
501 501 c, t dbSNP:779322298
502 502 a, g dbSNP:746197626
509 509 c, g dbSNP:564053771
523 523 c, t dbSNP:780257752
527 527 a, t dbSNP:137853589
528 528 g, t dbSNP:199743139
534 534 c, t dbSNP:138416154
537 537 -, g dbSNP:34885557
541 541 c, t dbSNP:773982918
552 552 g, t dbSNP:759361949
558 558 a, t dbSNP:767112052
559 559 a, g dbSNP:752363595
563 563 a, g dbSNP:370423261
567 567 c, g, t dbSNP:763509347
570 570 a, g dbSNP:149163610
571 571 c, g dbSNP:766949870
581 581 a, c, g dbSNP:555472522
589 589 -, a dbSNP:768238080
594 594 c, g dbSNP:201659307
603 603 a, g dbSNP:55751949
610 610 a, c, g dbSNP:578228524
613 613 c, t dbSNP:148036324
614 614 a, g dbSNP:775246080
632 632 c, t dbSNP:375764924
633 633 a, g dbSNP:768128006
635 635 g, t dbSNP:776358711
642 642 c, t dbSNP:761161501
643 643 c, t dbSNP:137853591
650 650 a, g dbSNP:764834643
651 651 a, c dbSNP:774068311
664 664 c, t dbSNP:772912966
668 668 a, t dbSNP:760096451
678 678 c, t dbSNP:768174256
679 679 a, g dbSNP:752961445
681 681 -, g dbSNP:773199410
699 699 c, t dbSNP:138740083
701 701 a, g dbSNP:545582783
705 705 g, t dbSNP:146181866
707 707 a, c dbSNP:757490714
712 712 c, t dbSNP:778952896
718 718 g, t dbSNP:750553595
726 726 c, t dbSNP:758301470
727 727 c, g, t dbSNP:779998350
729 729 g, t dbSNP:768501872
732 732 -, ctc dbSNP:762844937
733 733 c, t dbSNP:780913532
739 739 c, g dbSNP:45612445
740 740 a, t dbSNP:139116127
745 745 a, g dbSNP:368904522
749 749 c, g dbSNP:762578361
753 753 a, c, t dbSNP:372239376
756 756 a, g dbSNP:760885327
764 764 a, g, t dbSNP:61733974
776 776 a, g dbSNP:137853588
786 786 a, g, t dbSNP:373760692
788 788 a, t dbSNP:777504531
795 795 a, g dbSNP:187710792
808 808 a, c dbSNP:757031236
810 810 a, g dbSNP:778467590
815 815 c, t dbSNP:745412396
817 817 a, g dbSNP:769168117
818 818 c, t dbSNP:192866993
822 822 c, t dbSNP:748454786
827 827 c, t dbSNP:770138890
828 828 c, t dbSNP:773624428
829 829 c, t dbSNP:763137737
830 830 a, c dbSNP:766548642
849 849 a, g dbSNP:774446283
852 852 c, t dbSNP:759553322
853 853 a, g dbSNP:767427889
855 855 c, t dbSNP:368886148
866 866 c, g dbSNP:189032780
878 878 a, t dbSNP:775063859
886 886 a, c dbSNP:760671224
887 887 g, t dbSNP:137853592
899 899 c, t dbSNP:764170465
900 900 a, g dbSNP:373949480
914 914 a, c dbSNP:761535736
916 916 c, t dbSNP:370052455
917 917 a, g dbSNP:143915773
919 919 c, t dbSNP:374406482
921 921 a, g dbSNP:750049515
930 930 a, g dbSNP:758127060
937 937 c, t dbSNP:779515173
938 938 a, g dbSNP:777581985
946 946 a, g dbSNP:751109671
947 947 c, t dbSNP:577778473
950 950 a, g dbSNP:34006569
952 952 a, g dbSNP:756572785
966 966 c, t dbSNP:778270637
967 967 a, g dbSNP:368436227
971 971 -, ccc dbSNP:776611188
972 972 c, t dbSNP:771202086
973 973 c, t dbSNP:779404778
975 975 c, t dbSNP:746067638
976 976 a, g dbSNP:772392144
979 979 g, t dbSNP:775683935
981 981 a, g dbSNP:760869502
984 984 c, t dbSNP:372673156
987 987 a, c dbSNP:545313335
988 988 c, g, t dbSNP:148776797
992 992 c, g dbSNP:144792786
996 996 c, t dbSNP:750279480
999 999 c, t dbSNP:762482040
1001 1001 c, t dbSNP:111883335
1017 1017 a, c dbSNP:147966774
1034 1034 a, c dbSNP:748011645
1047 1047 a, c dbSNP:141832212
1048 1048 c, g dbSNP:568815926
1057 1057 a, g dbSNP:772842581
1065 1065 a, c dbSNP:762828840
1071 1071 a, g dbSNP:765954356
1077 1077 c, t dbSNP:773992574
1080 1080 c, t dbSNP:759248646
1087 1087 c, t dbSNP:767003992
1088 1088 a, g dbSNP:200348735
1089 1089 c, t dbSNP:757678936
1099 1099 a, g dbSNP:765807785
1113 1113 a, c, t dbSNP:750705511
1117 1117 a, c, g dbSNP:780358808
1118 1118 c, t dbSNP:755298493
1121 1121 a, c, t dbSNP:781099286
1123 1123 c, g, t dbSNP:200288919
1124 1124 a, c, g dbSNP:554920657
1127 1127 a, g dbSNP:770705206
1128 1128 a, g dbSNP:774187799
1129 1129 c, t dbSNP:759169790
1130 1130 a, g dbSNP:145390070
1131 1131 g, t dbSNP:201113602
1135 1135 c, t dbSNP:760257918
1136 1136 a, g dbSNP:572115942
1152 1152 a, c, g dbSNP:377396970
1156 1156 c, t dbSNP:751922915
1159 1159 a, g dbSNP:759992249
1160 1160 c, t dbSNP:767565421
1168 1168 c, t dbSNP:560414449
1169 1169 a, g dbSNP:756345604
1179 1179 a, g dbSNP:777884245
1184 1184 c, g dbSNP:753962410
1186 1186 c, t dbSNP:757148929
1187 1187 a, g dbSNP:778847073
1189 1189 c, t dbSNP:745609608
1190 1190 a, g dbSNP:771905827
1191 1191 c, t dbSNP:779780888
1195 1195 c, t dbSNP:367711698
1196 1196 a, g, t dbSNP:200724536
1205 1205 c, t dbSNP:761428508
1206 1206 c, t dbSNP:771505836
1220 1220 c, t dbSNP:775002634
1222 1222 a, g dbSNP:561316398
1225 1225 a, g dbSNP:375944529
1231 1231 c, t dbSNP:767906816
1232 1232 a, t dbSNP:753049419
1233 1233 c, t dbSNP:760917040
1235 1235 c, t dbSNP:764335210
1236 1236 a, g dbSNP:200852313
1237 1237 c, t dbSNP:757346673
1240 1240 c, t dbSNP:564686049
1241 1241 a, g dbSNP:532137455
1242 1242 g, t dbSNP:758327139
1249 1249 c, t dbSNP:779878553
1250 1250 a, g dbSNP:746809628
1254 1254 c, t dbSNP:201829095
1260 1260 a, c, t dbSNP:371914785
1261 1261 a, g dbSNP:151033581
1263 1263 c, t dbSNP:772911287
1264 1264 a, g dbSNP:746361026
1267 1267 a, t dbSNP:772493213
1271 1271 c, t dbSNP:775887057
1276 1276 c, g, t dbSNP:140913831
1277 1277 a, g dbSNP:776847234
1280 1280 c, t dbSNP:762089284
1281 1281 c, g dbSNP:200630126
1284 1284 c, t dbSNP:375398772
1285 1285 a, g dbSNP:371346868
1287 1287 a, g dbSNP:747926397
1288 1288 c, t dbSNP:766247639
1308 1308 a, g dbSNP:761514239
1317 1317 c, g dbSNP:764709039
1320 1320 a, g dbSNP:749979390
1321 1321 a, g dbSNP:755425990
1330 1330 a, g dbSNP:768078442
1332 1332 -, atg dbSNP:776735043
1336 1336 a, t dbSNP:776771755
1340 1340 c, g dbSNP:752935164
1341 1341 c, t dbSNP:756494047
1346 1346 a, g dbSNP:778056178
1354 1354 c, t dbSNP:754089234
1356 1356 c, t dbSNP:757592922
1359 1359 a, t dbSNP:779095608
1365 1365 c, t dbSNP:745967713
1380 1380 g, t dbSNP:771846341
1392 1392 c, g dbSNP:780047944
1407 1407 -, gccc dbSNP:761837008
1410 1410 c, t dbSNP:746987447
1411 1411 a, g dbSNP:768487396
1412 1412 g, t dbSNP:776341960
1414 1414 c, t dbSNP:761427924
1415 1415 a, c dbSNP:769471773
1418 1418 c, t dbSNP:772647889
1420 1420 g, t dbSNP:762452726
1425 1425 c, t dbSNP:765869402
1427 1427 a, g dbSNP:753233778
1430 1430 a, g dbSNP:761133096
1431 1431 a, c dbSNP:764487886
1450 1450 c, g dbSNP:754285539
1451 1451 c, t dbSNP:757506435
1454 1454 c, g dbSNP:779291857
1458 1458 c, t dbSNP:750463077
1461 1461 a, g dbSNP:368738097
1468 1468 c, t dbSNP:780058909
1472 1472 c, g dbSNP:746945674
1474 1474 a, g dbSNP:768520508
1479 1479 c, t dbSNP:780928772
1484 1484 a, g dbSNP:140609394
1486 1486 a, g dbSNP:769365649
1488 1488 a, g dbSNP:772844299
1491 1491 c, g dbSNP:762568050
1492 1492 a, c dbSNP:770450819
1494 1494 a, c dbSNP:773772541
1496 1496 a, g dbSNP:761204117
1499 1499 a, c, t dbSNP:144822491
1500 1500 a, g dbSNP:536695925
1505 1505 c, t dbSNP:151271860
1506 1506 a, g dbSNP:372278563
1512 1512 c, t dbSNP:758600890
1515 1515 a, c, g dbSNP:766453263
1530 1530 c, t dbSNP:573634467
1532 1532 c, g dbSNP:754899337
1533 1533 c, t dbSNP:781108578
1534 1534 a, g dbSNP:748146078
1538 1538 -, a dbSNP:769895659
1547 1547 c, t dbSNP:540907841
1548 1548 a, g dbSNP:777592720
1549 1549 c, t dbSNP:377047162
1550 1550 a, g dbSNP:745955837
1554 1554 c, g dbSNP:773861206
1556 1556 a, g dbSNP:745384074
1557 1557 -, agaggtcc dbSNP:773681543
1561 1561 a, g, t dbSNP:769218304
1573 1573 c, t dbSNP:762470096
1575 1575 c, g dbSNP:559195500
1576 1576 c, t dbSNP:146729147
1601 1601 c, g dbSNP:763198572
1604 1604 c, t dbSNP:766536164
1611 1611 a, g dbSNP:140316897
1625 1625 c, t dbSNP:755003711
1629 1629 c, t dbSNP:767639553
1636 1636 g, t dbSNP:752605159
1642 1642 -, gag dbSNP:754220889
1642 1642 a, g dbSNP:756083443
1649 1649 a, g dbSNP:777502771
1656 1656 a, c dbSNP:749059928
1667 1667 c, t dbSNP:756875437
1670 1670 c, t dbSNP:778436357
1675 1675 a, g dbSNP:745600848
1691 1691 a, g dbSNP:577779107
1735 1735 c, t dbSNP:140478640
1748 1748 a, g dbSNP:775190630
1755 1755 -, t dbSNP:766717522
1763 1763 a, g dbSNP:748637602
1764 1764 c, t dbSNP:563676045
1769 1769 -, ct dbSNP:755274906
1772 1772 a, g dbSNP:773581097
1782 1782 c, g dbSNP:200933996
1796 1796 c, t dbSNP:771388927
1802 1802 a, g dbSNP:774418752
1810 1810 c, t dbSNP:759799433
1816 1816 a, g dbSNP:767551827
1817 1817 c, t dbSNP:370274490
1820 1820 a, g dbSNP:368771114
1827 1827 a, g dbSNP:374671822
1830 1830 c, t dbSNP:150382749
1835 1835 a, c dbSNP:753602877
1849 1849 a, c dbSNP:561244435
1850 1850 c, t dbSNP:756999045
1854 1854 -, aaaagaaa dbSNP:759824996
1856 1856 a, g dbSNP:778827493
1858 1858 a, g dbSNP:201327122
1859 1859 -, aa dbSNP:35446957
1863 1863 -, ag dbSNP:201882666
1878 1878 a, g dbSNP:750024690
1884 1884 c, t dbSNP:758167193
1887 1887 a, c dbSNP:779673765
1894 1894 a, g dbSNP:4889505
1897 1897 c, g dbSNP:547331495
1905 1905 a, g dbSNP:749885797
1937 1937 c, t dbSNP:545271753
1976 1976 c, t dbSNP:149984900
1979 1979 a, g dbSNP:538862364
1983 1983 a, c dbSNP:190683100
2005 2005 g, t dbSNP:761527381
2029 2029 c, t dbSNP:569691884
2058 2058 a, c dbSNP:767189076
2119 2119 c, t dbSNP:750112532
2135 2135 a, g dbSNP:546197463
2144 2144 a, g dbSNP:147660704
2175 2175 -, tt dbSNP:758821993
2220 2220 g, t dbSNP:554975918
2253 2253 c, t dbSNP:748995449
2279 2279 a, t dbSNP:573325591
2290 2290 a, g dbSNP:369131280
2317 2317 -, g dbSNP:528589526
2320 2320 -, g dbSNP:367805411
2323 2323 a, g dbSNP:770371321
2324 2324 c, t dbSNP:778134113
2326 2326 a, g dbSNP:765925576
2327 2327 c, t dbSNP:771175523
2330 2330 a, g dbSNP:774815803
2335 2335 a, g dbSNP:759713220
2346 2346 g, t dbSNP:772301654
2348 2348 a, g dbSNP:534288126
2349 2349 c, t dbSNP:760747798

Target ORF information:

RefSeq Version NM_001172432
Organism Homo sapiens (human)
Definition Homo sapiens phosphorylase kinase, gamma 2 (testis) (PHKG2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001172432

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGACGCTGG ACGTGGGGCC GGAGGATGAG CTGCCCGACT GGGCCGCCGC CAAAGAGTTT 
TACCAGAAGT ACGACCCTAA GGACGTCATC GGCAGAGGAG TGAGCTCTGT GGTCCGCCGT
TGTGTTCATC GAGCTACTGG CCACGAGTTT GCGGTGAAGA TTATGGAAGT GACAGCTGAG
CGGCTGAGTC CTGAGCAGCT GGAGGAGGTG CGGGAAGCCA CACGGCGAGA GACACACATC
CTTCGCCAGG TCGCCGGCCA CCCCCACATC ATCACCCTCA TCGATTCCTA CGAGTCTTCT
AGCTTCATGT TCCTGGTGTT TGACCTGATG CGGAAGGGAG AGCTGTTTGA CTATCTCACA
GAGAAGGTGG CCCTCTCTGA AAAGGAAACC AGGTCCATCA TGCGGTCTCT GCTGGAAGCA
GTGAGCTTTC TCCATGCCAA CAACATTGTG CATCGAGATC TGAAGCCCGA GAATATTCTC
CTAGATGACA ATATGCAGAT CCGACTTTCA GATTTCGGGT TCTCCTGCCA CTTGGAACCT
GGCGAGAAGC TTCGAGAGTT GTGTGGGACC CCAGGGTATC TAGCGCCAGA GATCCTTAAA
TGCTCCATGG ATGAAACCCA CCCAGGCTAT GGCAAGGAGG TCGACCTCTG GGCCTGTGGG
GTGATCTTGT TCACACTCCT GGCTGGCTCG CCACCCTTCT GGCACCGGCG GCAGATCCTG
ATGTTACGCA TGATCATGGA GGGCCAGTAC CAGTTCAGTT CCCCCGAGTG GGATGACCGT
TCCAGCACTG TCAAAGACCT GATCTCCAGG CTGCTGCAGG TGGATCCTGA GGCACGCCTG
ACAGCTGAGC AGGCCCTACA GCACCCCTTC TTTGAGCGTT GTGAAGGCAG CCAACCCTGG
AACCTCACCC CCCGCCAGCG GTTCCGGGTG GCAGTGTGGA CAGTGCTGGC TGCTGGACGA
GTGGCCCTAA GCACCCATCG TGTACGGCCA CTGACCAAGA ATGCACTGTT GAGGGACCCT
TATGCGCTGC GGTCAGTGCG GCACCTCATC GACAACTGTG CCTTCCGGCT CTACGGGCAC
TGGATAAGGA AGCAGTGGAT TGGAAAGCTG ATGGCTTGTG TATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23652
Accession Version NM_000294.2
Sequence Information ORF Nucleotide Sequence (Length: 1221bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product phosphorylase b kinase gamma catalytic chain, liver/testis isoform isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC106886.3 and M31606.1. This sequence is a reference standard in the RefSeqGene project. On Feb 18, 2010 this sequence version replaced gi:4505784. Summary: Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9C, also known as autosomal liver glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M31606.1, AK289492.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGACGCTGG ACGTGGGGCC GGAGGATGAG CTGCCCGACT GGGCCGCCGC CAAAGAGTTT 
TACCAGAAGT ACGACCCTAA GGACGTCATC GGCAGAGGAG TGAGCTCTGT GGTCCGCCGT
TGTGTTCATC GAGCTACTGG CCACGAGTTT GCGGTGAAGA TTATGGAAGT GACAGCTGAG
CGGCTGAGTC CTGAGCAGCT GGAGGAGGTG CGGGAAGCCA CACGGCGAGA GACACACATC
CTTCGCCAGG TCGCCGGCCA CCCCCACATC ATCACCCTCA TCGATTCCTA CGAGTCTTCT
AGCTTCATGT TCCTGGTGTT TGACCTGATG CGGAAGGGAG AGCTGTTTGA CTATCTCACA
GAGAAGGTGG CCCTCTCTGA AAAGGAAACC AGGTCCATCA TGCGGTCTCT GCTGGAAGCA
GTGAGCTTTC TCCATGCCAA CAACATTGTG CATCGAGATC TGAAGCCCGA GAATATTCTC
CTAGATGACA ATATGCAGAT CCGACTTTCA GATTTCGGGT TCTCCTGCCA CTTGGAACCT
GGCGAGAAGC TTCGAGAGTT GTGTGGGACC CCAGGGTATC TAGCGCCAGA GATCCTTAAA
TGCTCCATGG ATGAAACCCA CCCAGGCTAT GGCAAGGAGG TCGACCTCTG GGCCTGTGGG
GTGATCTTGT TCACACTCCT GGCTGGCTCG CCACCCTTCT GGCACCGGCG GCAGATCCTG
ATGTTACGCA TGATCATGGA GGGCCAGTAC CAGTTCAGTT CCCCCGAGTG GGATGACCGT
TCCAGCACTG TCAAAGACCT GATCTCCAGG CTGCTGCAGG TGGATCCTGA GGCACGCCTG
ACAGCTGAGC AGGCCCTACA GCACCCCTTC TTTGAGCGTT GTGAAGGCAG CCAACCCTGG
AACCTCACCC CCCGCCAGCG GTTCCGGGTG GCAGTGTGGA CAGTGCTGGC TGCTGGACGA
GTGGCCCTAA GCACCCATCG TGTACGGCCA CTGACCAAGA ATGCACTGTT GAGGGACCCT
TATGCGCTGC GGTCAGTGCG GCACCTCATC GACAACTGTG CCTTCCGGCT CTACGGGCAC
TGGGTAAAGA AAGGGGAGCA GCAGAACCGG GCGGCTCTCT TTCAGCACCG GCCCCCTGGG
CCTTTTCCCA TCATGGGCCC TGAAGAGGAG GGAGACTCTG CTGCTATAAC TGAGGATGAG
GCCGTGCTTG TGCTGGGCTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000285.1
CDS211..1431
Misc Feature(1)247..1083(+)
Misc Feature(2)280..1083(+)
Misc Feature(3)298..792(+)
Misc Feature(4)298..723(+)
Misc Feature(5)310..792(+)
Misc Feature(6)718..786(+)
Misc Feature(7)1126..1200(+)
Misc Feature(8)1246..1320(+)
Exon (1)1..192
Gene:PHKG2
Gene Synonym:
Exon (2)193..305
Gene:PHKG2
Gene Synonym:
Exon (3)306..481
Gene:PHKG2
Gene Synonym:
Exon (4)482..536
Gene:PHKG2
Gene Synonym:
Exon (5)537..602
Gene:PHKG2
Gene Synonym:
Exon (6)603..766
Gene:PHKG2
Gene Synonym:
Exon (7)767..857
Gene:PHKG2
Gene Synonym:
Exon (8)858..1011
Gene:PHKG2
Gene Synonym:
Exon (9)1012..1137
Gene:PHKG2
Gene Synonym:
Exon (10)1138..5503
Gene:PHKG2
Gene Synonym:
Translation
Position Chain Variation Link
65 65 c, t dbSNP:536542470
97 97 c, t dbSNP:11863277
102 102 a, c dbSNP:566930196
151 151 c, g dbSNP:746968932
160 160 c, g dbSNP:112201430
183 183 c, g, t dbSNP:556945561
188 188 c, t dbSNP:11547255
190 190 c, g dbSNP:534374752
193 193 -, c dbSNP:754399010
197 197 c, g dbSNP:11547256
219 219 a, g dbSNP:780095616
222 222 c, t dbSNP:565389823
231 231 a, c, g dbSNP:200427925
232 232 g, t dbSNP:375063162
258 258 a, c dbSNP:747851738
278 278 a, c dbSNP:769648334
293 293 a, t dbSNP:777228104
301 301 a, g dbSNP:748940118
326 326 a, g dbSNP:373732367
329 329 a, g dbSNP:756077227
334 334 a, g dbSNP:777606340
337 337 c, t dbSNP:202177461
340 340 c, t dbSNP:137853590
341 341 a, g dbSNP:778737777
349 349 a, g dbSNP:373915359
352 352 c, t dbSNP:202145162
354 354 c, t dbSNP:141595425
355 355 a, g dbSNP:776935753
357 357 a, g dbSNP:527291776
362 362 c, t dbSNP:770253840
363 363 a, g dbSNP:368095671
366 366 a, g dbSNP:763340630
372 372 g, t dbSNP:199854449
379 379 g, t dbSNP:766541878
384 384 a, t dbSNP:56207641
385 385 a, g dbSNP:144930894
391 391 c, t dbSNP:201199884
392 392 a, g dbSNP:147950160
402 402 c, t dbSNP:372292588
421 421 c, t dbSNP:756052327
422 422 a, g dbSNP:777894749
424 424 a, g dbSNP:753644625
434 434 a, g dbSNP:538127151
437 437 a, g dbSNP:138299462
443 443 c, g dbSNP:745537506
445 445 c, t dbSNP:758053261
454 454 c, t dbSNP:779594016
455 455 a, g dbSNP:746541930
462 462 c, t dbSNP:568221601
463 463 a, g dbSNP:535265672
465 465 c, t dbSNP:775095281
466 466 a, g dbSNP:143983247
470 470 -, c dbSNP:764699954
474 474 c, g dbSNP:774768035
477 477 c, t dbSNP:759795602
498 498 c, t dbSNP:56029513
500 500 a, g dbSNP:757735184
501 501 c, t dbSNP:779322298
502 502 a, g dbSNP:746197626
509 509 c, g dbSNP:564053771
523 523 c, t dbSNP:780257752
527 527 a, t dbSNP:137853589
528 528 g, t dbSNP:199743139
534 534 c, t dbSNP:138416154
537 537 -, g dbSNP:34885557
541 541 c, t dbSNP:773982918
552 552 g, t dbSNP:759361949
558 558 a, t dbSNP:767112052
559 559 a, g dbSNP:752363595
563 563 a, g dbSNP:370423261
567 567 c, g, t dbSNP:763509347
570 570 a, g dbSNP:149163610
571 571 c, g dbSNP:766949870
581 581 a, c, g dbSNP:555472522
589 589 -, a dbSNP:768238080
594 594 c, g dbSNP:201659307
603 603 a, g dbSNP:55751949
610 610 a, c, g dbSNP:578228524
613 613 c, t dbSNP:148036324
614 614 a, g dbSNP:775246080
632 632 c, t dbSNP:375764924
633 633 a, g dbSNP:768128006
635 635 g, t dbSNP:776358711
642 642 c, t dbSNP:761161501
643 643 c, t dbSNP:137853591
650 650 a, g dbSNP:764834643
651 651 a, c dbSNP:774068311
664 664 c, t dbSNP:772912966
668 668 a, t dbSNP:760096451
678 678 c, t dbSNP:768174256
679 679 a, g dbSNP:752961445
681 681 -, g dbSNP:773199410
699 699 c, t dbSNP:138740083
701 701 a, g dbSNP:545582783
705 705 g, t dbSNP:146181866
707 707 a, c dbSNP:757490714
712 712 c, t dbSNP:778952896
718 718 g, t dbSNP:750553595
726 726 c, t dbSNP:758301470
727 727 c, g, t dbSNP:779998350
729 729 g, t dbSNP:768501872
732 732 -, ctc dbSNP:762844937
733 733 c, t dbSNP:780913532
739 739 c, g dbSNP:45612445
740 740 a, t dbSNP:139116127
745 745 a, g dbSNP:368904522
749 749 c, g dbSNP:762578361
753 753 a, c, t dbSNP:372239376
756 756 a, g dbSNP:760885327
764 764 a, g, t dbSNP:61733974
776 776 a, g dbSNP:137853588
786 786 a, g, t dbSNP:373760692
788 788 a, t dbSNP:777504531
795 795 a, g dbSNP:187710792
808 808 a, c dbSNP:757031236
810 810 a, g dbSNP:778467590
815 815 c, t dbSNP:745412396
817 817 a, g dbSNP:769168117
818 818 c, t dbSNP:192866993
822 822 c, t dbSNP:748454786
827 827 c, t dbSNP:770138890
828 828 c, t dbSNP:773624428
829 829 c, t dbSNP:763137737
830 830 a, c dbSNP:766548642
849 849 a, g dbSNP:774446283
852 852 c, t dbSNP:759553322
853 853 a, g dbSNP:767427889
855 855 c, t dbSNP:368886148
866 866 c, g dbSNP:189032780
878 878 a, t dbSNP:775063859
886 886 a, c dbSNP:760671224
887 887 g, t dbSNP:137853592
899 899 c, t dbSNP:764170465
900 900 a, g dbSNP:373949480
914 914 a, c dbSNP:761535736
916 916 c, t dbSNP:370052455
917 917 a, g dbSNP:143915773
919 919 c, t dbSNP:374406482
921 921 a, g dbSNP:750049515
930 930 a, g dbSNP:758127060
937 937 c, t dbSNP:779515173
938 938 a, g dbSNP:777581985
946 946 a, g dbSNP:751109671
947 947 c, t dbSNP:577778473
950 950 a, g dbSNP:34006569
952 952 a, g dbSNP:756572785
966 966 c, t dbSNP:778270637
967 967 a, g dbSNP:368436227
971 971 -, ccc dbSNP:776611188
972 972 c, t dbSNP:771202086
973 973 c, t dbSNP:779404778
975 975 c, t dbSNP:746067638
976 976 a, g dbSNP:772392144
979 979 g, t dbSNP:775683935
981 981 a, g dbSNP:760869502
984 984 c, t dbSNP:372673156
987 987 a, c dbSNP:545313335
988 988 c, g, t dbSNP:148776797
992 992 c, g dbSNP:144792786
996 996 c, t dbSNP:750279480
999 999 c, t dbSNP:762482040
1001 1001 c, t dbSNP:111883335
1017 1017 a, c dbSNP:147966774
1034 1034 a, c dbSNP:748011645
1047 1047 a, c dbSNP:141832212
1048 1048 c, g dbSNP:568815926
1057 1057 a, g dbSNP:772842581
1065 1065 a, c dbSNP:762828840
1071 1071 a, g dbSNP:765954356
1077 1077 c, t dbSNP:773992574
1080 1080 c, t dbSNP:759248646
1087 1087 c, t dbSNP:767003992
1088 1088 a, g dbSNP:200348735
1089 1089 c, t dbSNP:757678936
1099 1099 a, g dbSNP:765807785
1113 1113 a, c, t dbSNP:750705511
1117 1117 a, c, g dbSNP:780358808
1118 1118 c, t dbSNP:755298493
1121 1121 a, c, t dbSNP:781099286
1123 1123 c, g, t dbSNP:200288919
1124 1124 a, c, g dbSNP:554920657
1127 1127 a, g dbSNP:770705206
1128 1128 a, g dbSNP:774187799
1129 1129 c, t dbSNP:759169790
1130 1130 a, g dbSNP:145390070
1131 1131 g, t dbSNP:201113602
1135 1135 c, t dbSNP:760257918
1136 1136 a, g dbSNP:572115942
1152 1152 a, c, g dbSNP:377396970
1156 1156 c, t dbSNP:751922915
1159 1159 a, g dbSNP:759992249
1160 1160 c, t dbSNP:767565421
1168 1168 c, t dbSNP:560414449
1169 1169 a, g dbSNP:756345604
1179 1179 a, g dbSNP:777884245
1184 1184 c, g dbSNP:753962410
1186 1186 c, t dbSNP:757148929
1187 1187 a, g dbSNP:778847073
1189 1189 c, t dbSNP:745609608
1190 1190 a, g dbSNP:771905827
1191 1191 c, t dbSNP:779780888
1195 1195 c, t dbSNP:367711698
1196 1196 a, g, t dbSNP:200724536
1205 1205 c, t dbSNP:761428508
1206 1206 c, t dbSNP:771505836
1220 1220 c, t dbSNP:775002634
1222 1222 a, g dbSNP:561316398
1225 1225 a, g dbSNP:375944529
1231 1231 c, t dbSNP:767906816
1232 1232 a, t dbSNP:753049419
1233 1233 c, t dbSNP:760917040
1235 1235 c, t dbSNP:764335210
1236 1236 a, g dbSNP:200852313
1237 1237 c, t dbSNP:757346673
1240 1240 c, t dbSNP:564686049
1241 1241 a, g dbSNP:532137455
1242 1242 g, t dbSNP:758327139
1249 1249 c, t dbSNP:779878553
1250 1250 a, g dbSNP:746809628
1254 1254 c, t dbSNP:201829095
1260 1260 a, c, t dbSNP:371914785
1261 1261 a, g dbSNP:151033581
1263 1263 c, t dbSNP:772911287
1264 1264 a, g dbSNP:746361026
1267 1267 a, t dbSNP:772493213
1271 1271 c, t dbSNP:775887057
1276 1276 c, g, t dbSNP:140913831
1277 1277 a, g dbSNP:776847234
1280 1280 c, t dbSNP:762089284
1281 1281 c, g dbSNP:200630126
1284 1284 c, t dbSNP:375398772
1285 1285 a, g dbSNP:371346868
1287 1287 a, g dbSNP:747926397
1288 1288 c, t dbSNP:766247639
1303 1303 c, g dbSNP:556977075
1304 1304 c, g dbSNP:751308686
1317 1317 c, g dbSNP:754732907
1318 1318 c, t dbSNP:201376250
1319 1319 a, g dbSNP:747784842
1322 1322 c, t dbSNP:755877198
1323 1323 g, t dbSNP:777384232
1324 1324 a, g dbSNP:748777868
1325 1325 -, ct dbSNP:750723072
1327 1327 c, g dbSNP:772584191
1340 1340 a, g dbSNP:550674389
1344 1344 c, t dbSNP:747336431
1347 1347 c, t dbSNP:61731628
1348 1348 a, g dbSNP:777039348
1350 1350 c, g dbSNP:202215781
1362 1362 c, t dbSNP:762002530
1364 1364 c, t dbSNP:765485631
1368 1368 c, t dbSNP:773291409
1379 1379 a, g dbSNP:762954272
1384 1384 a, g dbSNP:766271272
1391 1391 c, g dbSNP:145746592
1394 1394 c, t dbSNP:141025408
1396 1396 a, g dbSNP:759599736
1400 1400 c, g dbSNP:767394756
1401 1401 c, t dbSNP:752576835
1413 1413 c, t dbSNP:369904042
1414 1414 a, g dbSNP:777315776
1416 1416 g, t dbSNP:753334153
1434 1434 c, g dbSNP:756727483
1435 1435 c, t dbSNP:548164881
1443 1443 c, t dbSNP:747457838
1445 1445 -, g dbSNP:758779510
1446 1446 a, g dbSNP:1804159
1447 1447 g, t dbSNP:781506704
1453 1453 c, t dbSNP:372172604
1468 1468 c, t dbSNP:769944084
1469 1469 c, t dbSNP:773310416
1470 1470 c, g dbSNP:763301997
1471 1471 -, aga dbSNP:779635900
1471 1471 a, g dbSNP:770901938
1476 1476 a, g dbSNP:774535429
1477 1477 a, g dbSNP:759511764
1478 1478 a, g dbSNP:767591090
1479 1479 a, g dbSNP:752489044
1502 1502 g, t dbSNP:376574340
1503 1503 c, g dbSNP:369544826
1504 1504 g, t dbSNP:139507813
1505 1505 c, g dbSNP:147632793
1506 1506 -, ctctggc dbSNP:71820772
1508 1508 -, ctggcc dbSNP:775441488
1509 1509 -, tggcctc dbSNP:56107910
1509 1509 -, tggcctc dbSNP:397792208
1512 1512 c, g dbSNP:77175815
1521 1521 c, g dbSNP:756251216
1539 1539 c, t dbSNP:534073457
1572 1572 a, c dbSNP:552438292
1574 1574 g, t dbSNP:533114328
1606 1606 a, g dbSNP:570646711
1618 1618 c, t dbSNP:1061190
1624 1624 a, c, g dbSNP:375389458
1628 1628 c, t dbSNP:551710249
1678 1678 a, g dbSNP:191968602
1679 1679 c, t dbSNP:150529725
1682 1682 a, g dbSNP:766522688
1703 1703 c, g dbSNP:574789707
1730 1730 c, t dbSNP:184619676
1751 1751 a, g dbSNP:189336168
1754 1754 c, g dbSNP:760562749
1760 1760 g, t dbSNP:763739487
1764 1764 c, t dbSNP:753431904
1773 1773 c, t dbSNP:756992033
1776 1776 a, g dbSNP:373040790
1783 1783 a, g dbSNP:749977249
1784 1784 a, t dbSNP:755440328
1785 1785 g, t dbSNP:781672472
1790 1790 a, c dbSNP:748444172
1796 1796 c, g, t dbSNP:753883532
1798 1798 -, g dbSNP:746708732
1818 1818 c, g dbSNP:778258937
1819 1819 a, t dbSNP:749449323
1830 1830 c, t dbSNP:771223442
1849 1849 c, t dbSNP:572438853
1850 1850 a, g dbSNP:776921895
1853 1853 c, g dbSNP:759709742
1868 1868 -, tgc dbSNP:754416154
1879 1879 c, t dbSNP:772079956
1886 1886 c, t dbSNP:775575214
1887 1887 a, g dbSNP:760762883
1889 1889 c, t dbSNP:200334105
1900 1900 c, t dbSNP:138413324
1901 1901 a, g, t dbSNP:182267845
1909 1909 a, c dbSNP:764975253
1911 1911 c, t dbSNP:1048703
1914 1914 c, g dbSNP:757905209
1919 1919 c, g dbSNP:765937812
1928 1928 c, t dbSNP:753185535
1932 1932 -, ct dbSNP:748023608
1937 1937 c, t dbSNP:756688945
1942 1942 a, g dbSNP:777968026
1950 1950 c, t dbSNP:576593404
1961 1961 c, t dbSNP:757370237
1963 1963 a, c, g, t dbSNP:201418865
1964 1964 a, g dbSNP:373334034
1968 1968 a, g dbSNP:746924858
1970 1970 c, t dbSNP:768683366
1971 1971 a, g dbSNP:186003632
1972 1972 a, t dbSNP:761692751
1979 1979 c, g dbSNP:764727003
1982 1982 c, t dbSNP:772844089
1986 1986 a, g dbSNP:199800099
1989 1989 g, t dbSNP:765849404
1993 1993 a, g dbSNP:751109759
1998 1998 a, g dbSNP:756537669
2007 2007 a, g dbSNP:57192967
2011 2011 a, g dbSNP:377700607
2014 2014 -, ga dbSNP:769920487
2015 2015 a, g dbSNP:560177152
2018 2018 g, t dbSNP:189371716
2024 2024 a, g, t dbSNP:149242536
2027 2027 c, t dbSNP:780254584
2034 2034 -, g dbSNP:773295686
2069 2069 a, g dbSNP:373555168
2080 2080 c, g dbSNP:538044343
2089 2089 c, t dbSNP:549706731
2100 2100 a, g dbSNP:111252867
2137 2137 g, t dbSNP:746992681
2138 2138 a, g dbSNP:144420827
2153 2153 a, g dbSNP:535538948
2172 2172 c, t dbSNP:376402103
2220 2220 a, g dbSNP:139412754
2234 2234 c, t dbSNP:181307107
2246 2246 a, t dbSNP:765087062
2257 2257 c, g dbSNP:144133909
2265 2265 c, t dbSNP:186181298
2339 2339 a, g dbSNP:758326509
2345 2345 a, g dbSNP:756773062
2349 2349 c, t dbSNP:190107307
2352 2352 a, g dbSNP:182778444
2366 2366 a, g dbSNP:527578872
2383 2383 c, t dbSNP:544043833
2397 2397 c, t dbSNP:201737213
2400 2400 -, t dbSNP:575308078
2412 2412 -, ttttt dbSNP:374158229
2421 2421 c, t dbSNP:562136515
2428 2428 c, t dbSNP:574621853
2434 2434 a, t dbSNP:185919468
2461 2461 a, g dbSNP:541925908
2500 2500 c, t dbSNP:777561828
2503 2503 c, t dbSNP:370287873
2514 2514 c, t dbSNP:560253671
2555 2555 c, t dbSNP:527291442
2559 2559 c, t dbSNP:371856479
2560 2560 c, g dbSNP:551838376
2574 2574 a, g dbSNP:564448699
2582 2582 c, t dbSNP:531497276
2589 2589 a, g dbSNP:549981689
2598 2598 c, t dbSNP:756702414
2620 2620 ga, tt dbSNP:386790273
2620 2620 g, t dbSNP:190827459
2621 2621 a, t dbSNP:184000563
2687 2687 c, t dbSNP:780524779
2706 2706 -, t dbSNP:551154426
2736 2736 c, t dbSNP:187853639
2746 2746 a, g dbSNP:374205730
2761 2761 -, t dbSNP:567237520
2762 2762 c, t dbSNP:192483412
2767 2767 a, g dbSNP:112113047
2781 2781 a, g dbSNP:539823111
2805 2805 a, g dbSNP:375235385
2811 2811 c, t dbSNP:769270614
2813 2813 a, g dbSNP:780705475
2816 2816 c, t dbSNP:182864479
2826 2826 c, t dbSNP:570370884
2827 2827 a, g dbSNP:745402254
2876 2876 -, tttg dbSNP:537559274
2876 2876 g, t dbSNP:755446232
2877 2877 -, tttg dbSNP:774843102
2908 2908 a, t dbSNP:537331206
2961 2961 c, t dbSNP:779411243
2983 2983 c, t dbSNP:376553532
3030 3030 a, g dbSNP:555958023
3033 3033 c, t dbSNP:574298869
3061 3061 g, t dbSNP:541595921
3068 3068 c, t dbSNP:555809513
3080 3080 c, t dbSNP:11645367
3099 3099 c, t dbSNP:769181220
3131 3131 c, t dbSNP:572165552
3155 3155 g, t dbSNP:7193412
3167 3167 c, t dbSNP:563909532
3168 3168 a, g dbSNP:531661509
3199 3199 c, t dbSNP:771085997
3273 3273 c, g dbSNP:7188077
3282 3282 -, cctt dbSNP:560822842
3294 3294 a, g dbSNP:747146679
3299 3299 c, t dbSNP:561615712
3306 3306 a, g dbSNP:776645687
3309 3309 c, t dbSNP:371457610
3314 3314 c, t dbSNP:769560917
3322 3322 c, g dbSNP:773037049
3325 3325 a, c dbSNP:762557343
3329 3329 a, g dbSNP:538805878
3334 3334 c, t dbSNP:187063168
3336 3336 c, t dbSNP:553935133
3340 3340 -, tggctc dbSNP:749042317
3342 3342 -, ct dbSNP:770878389
3353 3353 a, g dbSNP:759098573
3363 3363 c, g dbSNP:764439368
3373 3373 g, t dbSNP:144755146
3376 3376 -, ct dbSNP:775822711
3378 3378 c, g dbSNP:61746942
3382 3382 c, g dbSNP:765676123
3385 3385 -, g dbSNP:761003739
3385 3385 c, t dbSNP:750859134
3386 3386 a, g dbSNP:565882864
3389 3389 a, g dbSNP:780448832
3391 3391 a, g dbSNP:751538068
3395 3395 g, t dbSNP:755078275
3402 3402 c, t dbSNP:368395391
3408 3408 a, t dbSNP:141842194
3417 3417 c, t dbSNP:748092461
3418 3418 g, t dbSNP:769758772
3423 3423 c, t dbSNP:777504706
3443 3443 c, t dbSNP:749101763
3444 3444 a, g dbSNP:770511048
3455 3455 a, g dbSNP:374688295
3456 3456 c, t dbSNP:533260900
3457 3457 a, g dbSNP:774055979
3459 3459 a, c dbSNP:368458049
3461 3461 c, g dbSNP:370922410
3462 3462 c, t dbSNP:774912276
3469 3469 -, gccca dbSNP:750447347
3472 3472 c, t dbSNP:373898885
3474 3474 a, g dbSNP:765880267
3475 3475 c, g dbSNP:750771634
3476 3476 c, g, t dbSNP:368035673
3480 3480 a, g dbSNP:371228244
3488 3488 a, c dbSNP:755201655
3506 3506 c, t dbSNP:759543408
3507 3507 a, g dbSNP:551789158
3513 3513 c, g dbSNP:752747001
3522 3522 -, ca dbSNP:369330243
3529 3529 g, t dbSNP:755996498
3535 3535 a, g dbSNP:145518957
3539 3539 c, t dbSNP:762177394
3542 3542 c, t dbSNP:147982789
3543 3543 a, c dbSNP:770706959
3547 3547 c, t dbSNP:141615439
3548 3548 -, g dbSNP:761807384
3553 3553 a, t dbSNP:778632923
3570 3570 c, t dbSNP:374769997
3571 3571 a, g dbSNP:367827173
3582 3582 a, g dbSNP:775026044
3589 3589 a, g dbSNP:375995977
3591 3591 a, c dbSNP:770369435
3592 3592 g, t dbSNP:773862121
3599 3599 c, t dbSNP:763609894
3608 3608 c, t dbSNP:191838893
3612 3612 c, t dbSNP:556104200
3623 3623 g, t dbSNP:759760912
3624 3624 a, g dbSNP:767655301
3630 3630 a, g dbSNP:752763963
3631 3631 c, t dbSNP:756188707
3632 3632 a, g dbSNP:775634684
3636 3636 a, g dbSNP:373154657
3637 3637 a, g dbSNP:753763872
3639 3639 a, g dbSNP:757236599
3644 3644 -, ctc dbSNP:765679699
3652 3652 c, g dbSNP:778739275
3661 3661 a, c dbSNP:745682011
3661 3661 -, c dbSNP:750953688
3664 3664 c, g, t dbSNP:567910325
3665 3665 -, c dbSNP:35293079
3666 3666 -, cct dbSNP:374419590
3667 3667 a, c dbSNP:150540962
3678 3678 a, g dbSNP:768280328
3683 3683 -, cg dbSNP:763267334
3683 3683 c, t dbSNP:535279129
3684 3684 -, t dbSNP:766770966
3684 3684 a, g dbSNP:749778320
3690 3690 a, g dbSNP:771368044
3691 3691 c, t dbSNP:774725515
3695 3695 a, g dbSNP:759831279
3696 3696 c, t dbSNP:200532920
3701 3701 a, c dbSNP:767712119
3706 3706 c, t dbSNP:775682412
3711 3711 -, t dbSNP:751151606
3711 3711 a, g dbSNP:760943927
3716 3716 c, t dbSNP:139508776
3725 3725 c, t dbSNP:143239070
3737 3737 a, t dbSNP:757146657
3741 3741 c, t dbSNP:765109749
3743 3743 a, g dbSNP:147508732
3746 3746 a, g dbSNP:758136946
3747 3747 c, g dbSNP:140233714
3756 3756 c, t dbSNP:746634571
3758 3758 c, t dbSNP:754715082
3765 3765 a, g dbSNP:780804706
3767 3767 c, t dbSNP:376772095
3768 3768 a, g dbSNP:117306997
3774 3774 c, g dbSNP:779291634
3776 3776 a, c dbSNP:139907701
3781 3781 a, g dbSNP:148722707
3782 3782 a, g dbSNP:775882763
3793 3793 c, g dbSNP:760858040
3794 3794 a, g dbSNP:768897775
3796 3796 -, gactcg dbSNP:754717485
3798 3798 -, ctc dbSNP:780563774
3800 3800 c, g, t dbSNP:543365803
3801 3801 a, g dbSNP:373928064
3808 3808 c, g dbSNP:750326712
3812 3812 c, g dbSNP:763008720
3814 3814 g, t dbSNP:766242065
3819 3819 a, c dbSNP:751429905
3821 3821 c, t dbSNP:754624847
3825 3825 c, t dbSNP:561652243
3826 3826 a, g dbSNP:370346934
3842 3842 c, t dbSNP:189191189
3845 3845 c, t dbSNP:777397325
3866 3866 a, c dbSNP:746337535
3871 3871 a, g dbSNP:772676090
3877 3877 a, t dbSNP:780358480
3878 3878 a, c dbSNP:747397298
3890 3890 c, g dbSNP:768809722
3896 3896 g, t dbSNP:776847168
3901 3901 a, g dbSNP:762166233
3912 3912 g, t dbSNP:769752076
3914 3914 -, ga dbSNP:752193448
3918 3918 a, g dbSNP:773378624
3920 3920 a, g dbSNP:762757340
3921 3921 a, t dbSNP:766437980
3922 3922 c, g dbSNP:751338113
3928 3928 a, g dbSNP:759409260
3929 3929 a, g dbSNP:767486727
3932 3932 a, c dbSNP:373882523
3943 3943 -, a dbSNP:542596716
3947 3947 a, g dbSNP:755838861
3950 3950 -, aa dbSNP:777811070
3956 3956 c, t dbSNP:62622830
3957 3957 c, t dbSNP:201836721
3966 3966 c, t dbSNP:758854757
3978 3978 a, g dbSNP:780552714
3979 3979 a, c dbSNP:747311389
3980 3980 c, g dbSNP:749891103
3981 3981 c, g dbSNP:755336129
3989 3989 a, c dbSNP:781596696
3993 3993 a, g dbSNP:748299636
3994 3994 -, a dbSNP:749276025
4000 4000 -, ag dbSNP:770824963
4002 4002 a, g dbSNP:142254614
4007 4007 a, t dbSNP:773114148
4018 4018 c, t dbSNP:749395777
4019 4019 a, c, g dbSNP:374340751
4022 4022 a, t dbSNP:759605425
4024 4024 -, cac dbSNP:778823423
4024 4024 c, t dbSNP:367914019
4025 4025 a, c, g dbSNP:775394419
4027 4027 c, t dbSNP:151283168
4034 4034 c, t dbSNP:753229558
4035 4035 a, g dbSNP:756766317
4052 4052 c, t dbSNP:764799196
4057 4057 a, g dbSNP:563759395
4077 4077 a, g dbSNP:376622488
4085 4085 -, ct dbSNP:747188865
4091 4091 a, g dbSNP:755531693
4101 4101 c, t dbSNP:781382632
4103 4103 c, t dbSNP:530811946
4109 4109 g, t dbSNP:549392115
4112 4112 c, g, t dbSNP:778058702
4122 4122 c, g dbSNP:755641514
4124 4124 c, t dbSNP:377104428
4127 4127 a, t dbSNP:771119546
4139 4139 a, t dbSNP:774622300
4146 4146 c, t dbSNP:779391296
4148 4148 g, t dbSNP:745768787
4152 4152 a, g dbSNP:772168145
4156 4156 c, t dbSNP:567997789
4172 4172 c, t dbSNP:748697681
4243 4243 -, acaag dbSNP:760995706
4256 4256 a, g dbSNP:575930265
4260 4260 a, c dbSNP:758742628
4265 4265 a, c dbSNP:760824721
4269 4269 c, t dbSNP:142375933
4311 4311 g, t dbSNP:193252316
4317 4317 c, t dbSNP:539188179
4345 4345 a, g dbSNP:557578179
4355 4355 a, g dbSNP:778069910
4358 4358 c, g dbSNP:565338762
4376 4376 a, c dbSNP:376287076
4380 4380 a, g dbSNP:753394598
4405 4405 a, g dbSNP:775099923
4411 4411 a, t dbSNP:747336871
4423 4423 -, tgtgc dbSNP:768853154
4423 4423 g, t dbSNP:760533846
4424 4424 a, g dbSNP:771123082
4429 4429 a, g dbSNP:763588019
4435 4435 g, t dbSNP:185728673
4445 4445 c, g dbSNP:776344669
4460 4460 a, g dbSNP:761514239
4469 4469 c, g dbSNP:764709039
4472 4472 a, g dbSNP:749979390
4473 4473 a, g dbSNP:755425990
4482 4482 a, g dbSNP:768078442
4484 4484 -, atg dbSNP:776735043
4488 4488 a, t dbSNP:776771755
4492 4492 c, g dbSNP:752935164
4493 4493 c, t dbSNP:756494047
4498 4498 a, g dbSNP:778056178
4506 4506 c, t dbSNP:754089234
4508 4508 c, t dbSNP:757592922
4511 4511 a, t dbSNP:779095608
4517 4517 c, t dbSNP:745967713
4532 4532 g, t dbSNP:771846341
4544 4544 c, g dbSNP:780047944
4559 4559 -, gccc dbSNP:761837008
4562 4562 c, t dbSNP:746987447
4563 4563 a, g dbSNP:768487396
4564 4564 g, t dbSNP:776341960
4566 4566 c, t dbSNP:761427924
4567 4567 a, c dbSNP:769471773
4570 4570 c, t dbSNP:772647889
4572 4572 g, t dbSNP:762452726
4577 4577 c, t dbSNP:765869402
4579 4579 a, g dbSNP:753233778
4582 4582 a, g dbSNP:761133096
4583 4583 a, c dbSNP:764487886
4602 4602 c, g dbSNP:754285539
4603 4603 c, t dbSNP:757506435
4606 4606 c, g dbSNP:779291857
4610 4610 c, t dbSNP:750463077
4613 4613 a, g dbSNP:368738097
4620 4620 c, t dbSNP:780058909
4624 4624 c, g dbSNP:746945674
4626 4626 a, g dbSNP:768520508
4631 4631 c, t dbSNP:780928772
4636 4636 a, g dbSNP:140609394
4638 4638 a, g dbSNP:769365649
4640 4640 a, g dbSNP:772844299
4643 4643 c, g dbSNP:762568050
4644 4644 a, c dbSNP:770450819
4646 4646 a, c dbSNP:773772541
4648 4648 a, g dbSNP:761204117
4651 4651 a, c, t dbSNP:144822491
4652 4652 a, g dbSNP:536695925
4657 4657 c, t dbSNP:151271860
4658 4658 a, g dbSNP:372278563
4664 4664 c, t dbSNP:758600890
4667 4667 a, c, g dbSNP:766453263
4682 4682 c, t dbSNP:573634467
4684 4684 c, g dbSNP:754899337
4685 4685 c, t dbSNP:781108578
4686 4686 a, g dbSNP:748146078
4690 4690 -, a dbSNP:769895659
4699 4699 c, t dbSNP:540907841
4700 4700 a, g dbSNP:777592720
4701 4701 c, t dbSNP:377047162
4702 4702 a, g dbSNP:745955837
4706 4706 c, g dbSNP:773861206
4708 4708 a, g dbSNP:745384074
4709 4709 -, agaggtcc dbSNP:773681543
4713 4713 a, g, t dbSNP:769218304
4725 4725 c, t dbSNP:762470096
4727 4727 c, g dbSNP:559195500
4728 4728 c, t dbSNP:146729147
4753 4753 c, g dbSNP:763198572
4756 4756 c, t dbSNP:766536164
4763 4763 a, g dbSNP:140316897
4777 4777 c, t dbSNP:755003711
4781 4781 c, t dbSNP:767639553
4788 4788 g, t dbSNP:752605159
4794 4794 -, gag dbSNP:754220889
4794 4794 a, g dbSNP:756083443
4801 4801 a, g dbSNP:777502771
4808 4808 a, c dbSNP:749059928
4819 4819 c, t dbSNP:756875437
4822 4822 c, t dbSNP:778436357
4827 4827 a, g dbSNP:745600848
4843 4843 a, g dbSNP:577779107
4887 4887 c, t dbSNP:140478640
4900 4900 a, g dbSNP:775190630
4907 4907 -, t dbSNP:766717522
4915 4915 a, g dbSNP:748637602
4916 4916 c, t dbSNP:563676045
4921 4921 -, ct dbSNP:755274906
4924 4924 a, g dbSNP:773581097
4934 4934 c, g dbSNP:200933996
4948 4948 c, t dbSNP:771388927
4954 4954 a, g dbSNP:774418752
4962 4962 c, t dbSNP:759799433
4968 4968 a, g dbSNP:767551827
4969 4969 c, t dbSNP:370274490
4972 4972 a, g dbSNP:368771114
4979 4979 a, g dbSNP:374671822
4982 4982 c, t dbSNP:150382749
4987 4987 a, c dbSNP:753602877
5001 5001 a, c dbSNP:561244435
5002 5002 c, t dbSNP:756999045
5006 5006 -, aaaagaaa dbSNP:759824996
5008 5008 a, g dbSNP:778827493
5010 5010 a, g dbSNP:201327122
5011 5011 -, aa dbSNP:35446957
5015 5015 -, ag dbSNP:201882666
5030 5030 a, g dbSNP:750024690
5036 5036 c, t dbSNP:758167193
5039 5039 a, c dbSNP:779673765
5046 5046 a, g dbSNP:4889505
5049 5049 c, g dbSNP:547331495
5057 5057 a, g dbSNP:749885797
5089 5089 c, t dbSNP:545271753
5128 5128 c, t dbSNP:149984900
5131 5131 a, g dbSNP:538862364
5135 5135 a, c dbSNP:190683100
5157 5157 g, t dbSNP:761527381
5181 5181 c, t dbSNP:569691884
5210 5210 a, c dbSNP:767189076
5271 5271 c, t dbSNP:750112532
5287 5287 a, g dbSNP:546197463
5296 5296 a, g dbSNP:147660704
5327 5327 -, tt dbSNP:758821993
5372 5372 g, t dbSNP:554975918
5405 5405 c, t dbSNP:748995449
5431 5431 a, t dbSNP:573325591
5442 5442 a, g dbSNP:369131280
5469 5469 -, g dbSNP:528589526
5472 5472 -, g dbSNP:367805411
5475 5475 a, g dbSNP:770371321
5476 5476 c, t dbSNP:778134113
5478 5478 a, g dbSNP:765925576
5479 5479 c, t dbSNP:771175523
5482 5482 a, g dbSNP:774815803
5487 5487 a, g dbSNP:759713220
5498 5498 g, t dbSNP:772301654
5500 5500 a, g dbSNP:534288126
5501 5501 c, t dbSNP:760747798

Target ORF information:

RefSeq Version NM_000294
Organism Homo sapiens (human)
Definition Homo sapiens phosphorylase kinase, gamma 2 (testis) (PHKG2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000294

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGACGCTGG ACGTGGGGCC GGAGGATGAG CTGCCCGACT GGGCCGCCGC CAAAGAGTTT 
TACCAGAAGT ACGACCCTAA GGACGTCATC GGCAGAGGAG TGAGCTCTGT GGTCCGCCGT
TGTGTTCATC GAGCTACTGG CCACGAGTTT GCGGTGAAGA TTATGGAAGT GACAGCTGAG
CGGCTGAGTC CTGAGCAGCT GGAGGAGGTG CGGGAAGCCA CACGGCGAGA GACACACATC
CTTCGCCAGG TCGCCGGCCA CCCCCACATC ATCACCCTCA TCGATTCCTA CGAGTCTTCT
AGCTTCATGT TCCTGGTGTT TGACCTGATG CGGAAGGGAG AGCTGTTTGA CTATCTCACA
GAGAAGGTGG CCCTCTCTGA AAAGGAAACC AGGTCCATCA TGCGGTCTCT GCTGGAAGCA
GTGAGCTTTC TCCATGCCAA CAACATTGTG CATCGAGATC TGAAGCCCGA GAATATTCTC
CTAGATGACA ATATGCAGAT CCGACTTTCA GATTTCGGGT TCTCCTGCCA CTTGGAACCT
GGCGAGAAGC TTCGAGAGTT GTGTGGGACC CCAGGGTATC TAGCGCCAGA GATCCTTAAA
TGCTCCATGG ATGAAACCCA CCCAGGCTAT GGCAAGGAGG TCGACCTCTG GGCCTGTGGG
GTGATCTTGT TCACACTCCT GGCTGGCTCG CCACCCTTCT GGCACCGGCG GCAGATCCTG
ATGTTACGCA TGATCATGGA GGGCCAGTAC CAGTTCAGTT CCCCCGAGTG GGATGACCGT
TCCAGCACTG TCAAAGACCT GATCTCCAGG CTGCTGCAGG TGGATCCTGA GGCACGCCTG
ACAGCTGAGC AGGCCCTACA GCACCCCTTC TTTGAGCGTT GTGAAGGCAG CCAACCCTGG
AACCTCACCC CCCGCCAGCG GTTCCGGGTG GCAGTGTGGA CAGTGCTGGC TGCTGGACGA
GTGGCCCTAA GCACCCATCG TGTACGGCCA CTGACCAAGA ATGCACTGTT GAGGGACCCT
TATGCGCTGC GGTCAGTGCG GCACCTCATC GACAACTGTG CCTTCCGGCT CTACGGGCAC
TGGGTAAAGA AAGGGGAGCA GCAGAACCGG GCGGCTCTCT TTCAGCACCG GCCCCCTGGG
CCTTTTCCCA TCATGGGCCC TGAAGAGGAG GGAGACTCTG CTGCTATAAC TGAGGATGAG
GCCGTGCTTG TGCTGGGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.