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PITX2 paired-like homeodomain 2 [Homo sapiens (human)]

Gene Symbol PITX2
Entrez Gene ID 5308
Full Name paired-like homeodomain 2
Synonyms ARP1, Brx1, IDG2, IGDS, IGDS2, IHG2, IRID2, Otlx2, PTX2, RGS, RIEG, RIEG1, RS
General protein information
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

4

4q25

Summary This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. The encoded protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. This protein plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdominal organs, and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this gene are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

601542

Disorder Html: Axenfeld-Rieger syndrome, type 1, 180500 (3); Iridogoniodysgenesis,





The following PITX2 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PITX2 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu17508 XM_006714235 PREDICTED: Homo sapiens paired-like homeodomain 2 (PITX2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu17480 XM_011532027 PREDICTED: Homo sapiens paired-like homeodomain 2 (PITX2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$199
OHu17508 NM_001204397 Homo sapiens paired-like homeodomain 2 (PITX2), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu17508 NM_001204398 Homo sapiens paired-like homeodomain 2 (PITX2), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu17480 NM_001204399 Homo sapiens paired-like homeodomain 2 (PITX2), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$199
OHu17508 NM_153426 Homo sapiens paired-like homeodomain 2 (PITX2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $309
OHu17480 NM_153427 Homo sapiens paired-like homeodomain 2 (PITX2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$309
OHu17360 NM_000325 Homo sapiens paired-like homeodomain 2 (PITX2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$199

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu17508
Accession Version XM_006714235.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pituitary homeobox 2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_016354.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578809166. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACCA ACTGCCGCAA ACTGGTGTCG GCGTGTGTGC AATTAGGCGT GCAGCCGGCG 
GCCGTTGAAT GTCTCTTCTC CAAAGACTCC GAAATCAAAA AGGTCGAGTT CACGGACTCT
CCTGAGAGCC GAAAAGAGGC AGCCAGCAGC AAGTTCTTCC CGCGGCAGCA TCCTGGCGCC
AATGAGAAAG ATAAAAGCCA GCAGGGGAAG AATGAGGACG TGGGCGCCGA GGACCCGTCT
AAGAAGAAGC GGCAAAGGCG GCAGCGGACT CACTTTACCA GCCAGCAGCT CCAGGAGCTG
GAGGCCACTT TCCAGAGGAA CCGCTACCCG GACATGTCCA CACGCGAAGA AATCGCTGTG
TGGACCAACC TTACGGAAGC CCGAGTCCGG GTTTGGTTCA AGAATCGTCG GGCCAAATGG
AGAAAGAGGG AGCGCAACCA GCAGGCCGAG CTATGCAAGA ATGGCTTCGG GCCGCAGTTC
AATGGGCTCA TGCAGCCCTA CGACGACATG TACCCAGGCT ATTCCTACAA CAACTGGGCC
GCCAAGGGCC TTACATCCGC CTCCCTATCC ACCAAGAGCT TCCCCTTCTT CAACTCTATG
AACGTCAACC CCCTGTCATC ACAGAGCATG TTTTCCCCAC CCAACTCTAT CTCGTCCATG
AGCATGTCGT CCAGCATGGT GCCCTCAGCA GTGACAGGCG TCCCGGGCTC CAGTCTCAAC
AGCCTGAATA ACTTGAACAA CCTGAGTAGC CCGTCGCTGA ATTCCGCGGT GCCGACGCCT
GCCTGTCCTT ACGCGCCGCC GACTCCTCCG TATGTTTATA GGGACACGTG TAACTCGAGC
CTGGCCAGCC TGAGACTGAA AGCAAAGCAG CACTCCAGCT TCGGCTACGC CAGCGTGCAG
AACCCGGCCT CCAACCTGAG TGCTTGCCAG TATGCAGTGG ACCGGCCCGT GTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006714298.1
CDS30..983
Misc Feature(1)285..461(+)
Misc Feature(2)285..455(+)
Misc Feature(3)291..443(+)
Misc Feature(4)849..905(+)
Translation
Position Chain Variation Link
9 9 c, g dbSNP:3796898
16 16 c, g dbSNP:554967575
31 31 g, t dbSNP:75334582
35 35 a, g dbSNP:376818069
42 42 c, g, t dbSNP:200683912
53 53 a, g, t dbSNP:143778014
56 56 a, g, t dbSNP:531925274
57 57 a, t dbSNP:139906074
59 59 c, g dbSNP:746215581
61 61 c, t dbSNP:373848340
62 62 c, g dbSNP:370048347
65 65 c, t dbSNP:749410556
66 66 c, g dbSNP:1131942
67 67 g, t dbSNP:374524467
73 73 c, t dbSNP:778111934
77 77 c, g dbSNP:763636556
78 78 g, t dbSNP:760317329
88 88 c, t dbSNP:150684621
93 93 c, g dbSNP:771505272
96 96 a, g dbSNP:763204161
109 109 c, t dbSNP:201299310
114 114 g, t dbSNP:770173811
133 133 c, t dbSNP:748305151
135 135 a, g dbSNP:781408424
141 141 a, g dbSNP:753677423
143 143 g, t dbSNP:200782789
144 144 g, t dbSNP:779828091
150 150 c, g dbSNP:758414076
163 163 a, g dbSNP:750353276
165 165 c, g dbSNP:565709983
182 182 a, g dbSNP:374552405
194 194 a, g dbSNP:142015013
200 200 a, t dbSNP:753524953
201 201 c, g dbSNP:763902543
206 206 a, c, t dbSNP:752299951
211 211 a, t dbSNP:766958445
212 212 c, t dbSNP:763475806
221 221 c, t dbSNP:370588233
229 229 a, c, t dbSNP:753935193
230 230 a, g dbSNP:756527247
232 232 a, c, g dbSNP:201628949
240 240 a, c dbSNP:781343571
242 242 a, t dbSNP:755404910
249 249 a, g dbSNP:535881495
253 253 a, g dbSNP:766774222
256 256 c, g dbSNP:763095137
257 257 c, g dbSNP:767533645
258 258 a, g dbSNP:765382079
276 276 -, aag dbSNP:770651665
280 280 a, c, g dbSNP:28936409
281 281 a, g dbSNP:149288560
290 290 g, t dbSNP:768386257
306 306 a, c dbSNP:760524995
311 311 c, t dbSNP:775089001
324 324 c, g dbSNP:1051887
328 328 a, t dbSNP:104893857
352 352 a, g dbSNP:104893862
369 369 a, c dbSNP:104893858
373 373 a, g dbSNP:104893861
410 410 c, t dbSNP:771757537
414 414 c, g dbSNP:121909249
416 416 c, g dbSNP:745540247
417 417 c, t dbSNP:121909248
424 424 g, t dbSNP:772800095
429 429 a, g dbSNP:387906810
437 437 c, t dbSNP:769461476
439 439 c, g dbSNP:104893859
451 451 a, g dbSNP:747454924
452 452 a, g dbSNP:138593200
458 458 a, g dbSNP:776157834
463 463 g, t dbSNP:772313984
464 464 c, t dbSNP:770354450
470 470 a, g dbSNP:746315869
476 476 a, c dbSNP:778975957
485 485 c, t dbSNP:757448249
494 494 c, t dbSNP:374404013
496 496 g, t dbSNP:777760630
497 497 c, t dbSNP:756196786
503 503 g, t dbSNP:752617007
506 506 a, g dbSNP:781170593
511 511 a, g dbSNP:754698054
529 529 a, g dbSNP:751418961
533 533 c, t dbSNP:113012179
538 538 c, t dbSNP:766110695
543 543 c, t dbSNP:527498517
556 556 a, g dbSNP:750107133
566 566 a, g dbSNP:104893860
570 570 a, g dbSNP:77144743
575 575 a, g dbSNP:781375019
578 578 c, t dbSNP:776139719
581 581 c, t dbSNP:772679916
584 584 a, t dbSNP:759978846
588 588 a, g dbSNP:193920830
594 594 c, g dbSNP:774733901
596 596 a, c, g dbSNP:749452484
601 601 c, g dbSNP:544781282
602 602 c, g dbSNP:577700410
613 613 a, c dbSNP:748266578
618 618 g, t dbSNP:780968713
620 620 c, t dbSNP:754895399
621 621 a, g dbSNP:751484586
622 622 a, g dbSNP:779833505
626 626 g, t dbSNP:35946364
627 627 a, g dbSNP:138163892
632 632 c, t dbSNP:765053157
642 642 c, t dbSNP:761378970
647 647 a, t dbSNP:141176394
659 659 c, g dbSNP:763539767
664 664 c, g dbSNP:760018349
678 678 a, g dbSNP:774730294
679 679 g, t dbSNP:771311013
681 681 c, t dbSNP:763373195
683 683 a, g dbSNP:140541021
686 686 c, t dbSNP:201319333
689 689 a, g dbSNP:748391268
692 692 c, t dbSNP:781291656
693 693 a, g dbSNP:768622921
703 703 a, g dbSNP:746908680
728 728 c, t dbSNP:555260580
729 729 a, g dbSNP:758267195
736 736 a, g dbSNP:146807994
737 737 c, t dbSNP:143714974
746 746 c, g dbSNP:756926743
763 763 g, t dbSNP:536920164
782 782 a, g dbSNP:763598663
787 787 c, t dbSNP:755635155
788 788 c, g dbSNP:752099801
794 794 c, g dbSNP:766833864
795 795 c, g, t dbSNP:149181425
803 803 g, t dbSNP:145355581
806 806 a, c, g dbSNP:776732876
816 816 a, c, t dbSNP:747126705
817 817 c, t dbSNP:775470219
820 820 a, t dbSNP:371238455
821 821 c, t dbSNP:745827821
824 824 a, g dbSNP:778779105
825 825 a, c dbSNP:376861814
826 826 c, t dbSNP:777541682
827 827 a, g dbSNP:148191851
829 829 a, c dbSNP:777353401
841 841 a, g dbSNP:755618053
845 845 a, t dbSNP:752197021
855 855 a, g dbSNP:374542009
857 857 a, g dbSNP:767036406
870 870 c, t dbSNP:139401187
874 874 c, t dbSNP:750934780
875 875 c, t dbSNP:573990899
914 914 c, g dbSNP:1051888
915 915 c, t dbSNP:762224214
917 917 c, t dbSNP:776910041
923 923 c, g dbSNP:764265570
924 924 a, g dbSNP:760790139
932 932 g, t dbSNP:2739199
943 943 a, g dbSNP:775387051
944 944 c, t dbSNP:772278492
949 949 a, g dbSNP:571758306
958 958 a, g dbSNP:774485351
973 973 c, g dbSNP:770843364
977 977 a, c, t dbSNP:147543001
978 978 c, g, t dbSNP:747865049
986 986 c, t dbSNP:780613951
988 988 a, c dbSNP:754498609
989 989 a, g dbSNP:750917268
997 997 c, t dbSNP:779465924
998 998 c, g dbSNP:549958385
1000 1000 c, t dbSNP:754207409
1001 1001 a, g dbSNP:764545113
1007 1007 c, g dbSNP:1051889
1014 1014 a, c dbSNP:760772767
1015 1015 g, t dbSNP:752977430
1019 1019 c, g, t dbSNP:201933531
1025 1025 a, g dbSNP:774397515
1027 1027 a, g dbSNP:771039657
1034 1034 a, c, t dbSNP:772842482
1038 1038 c, t dbSNP:750221534
1090 1090 a, c dbSNP:752553102
1096 1096 -, a dbSNP:755301025
1102 1102 a, t dbSNP:765040142
1159 1159 a, t dbSNP:567517676
1164 1164 c, t dbSNP:564793153
1191 1191 a, g dbSNP:548886681
1211 1211 c, g dbSNP:767478639
1234 1234 g, t dbSNP:527607188
1298 1298 c, t dbSNP:754978001
1308 1308 c, t dbSNP:559998304
1323 1323 a, g dbSNP:551209662
1337 1337 a, g dbSNP:761836715
1347 1347 a, g dbSNP:532938712
1357 1357 c, t dbSNP:562612539
1416 1416 a, g dbSNP:543939301
1437 1437 c, t dbSNP:6533526
1438 1438 g, t dbSNP:561697335
1454 1454 a, g dbSNP:75911264
1473 1473 c, t dbSNP:144573985
1492 1492 -, attat dbSNP:754182898
1492 1492 a, g dbSNP:554499964
1500 1500 -, tattat dbSNP:764176907
1500 1500 -, tat dbSNP:775662096
1502 1502 -, tat dbSNP:569839843
1503 1503 -, tat dbSNP:561702585
1505 1505 c, t dbSNP:188349821
1540 1540 c, g dbSNP:2739201
1542 1542 c, g dbSNP:2739202
1558 1558 c, t dbSNP:759153090
1573 1573 -, ta dbSNP:200739974
1576 1576 a, g dbSNP:760397096
1580 1580 a, g dbSNP:772929558
1614 1614 -, att dbSNP:767699594
1670 1670 a, g dbSNP:185925533

Target ORF information:

RefSeq Version XM_006714235
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens paired-like homeodomain 2 (PITX2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006714235

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACCA ACTGCCGCAA ACTGGTGTCG GCGTGTGTGC AATTAGGCGT GCAGCCGGCG 
GCCGTTGAAT GTCTCTTCTC CAAAGACTCC GAAATCAAAA AGGTCGAGTT CACGGACTCT
CCTGAGAGCC GAAAAGAGGC AGCCAGCAGC AAGTTCTTCC CGCGGCAGCA TCCTGGCGCC
AATGAGAAAG ATAAAAGCCA GCAGGGGAAG AATGAGGACG TGGGCGCCGA GGACCCGTCT
AAGAAGAAGC GGCAAAGGCG GCAGCGGACT CACTTTACCA GCCAGCAGCT CCAGGAGCTG
GAGGCCACTT TCCAGAGGAA CCGCTACCCG GACATGTCCA CACGCGAAGA AATCGCTGTG
TGGACCAACC TTACGGAAGC CCGAGTCCGG GTTTGGTTCA AGAATCGTCG GGCCAAATGG
AGAAAGAGGG AGCGCAACCA GCAGGCCGAG CTATGCAAGA ATGGCTTCGG GCCGCAGTTC
AATGGGCTCA TGCAGCCCTA CGACGACATG TACCCAGGCT ATTCCTACAA CAACTGGGCC
GCCAAGGGCC TTACATCCGC CTCCCTATCC ACCAAGAGCT TCCCCTTCTT CAACTCTATG
AACGTCAACC CCCTGTCATC ACAGAGCATG TTTTCCCCAC CCAACTCTAT CTCGTCCATG
AGCATGTCGT CCAGCATGGT GCCCTCAGCA GTGACAGGCG TCCCGGGCTC CAGTCTCAAC
AGCCTGAATA ACTTGAACAA CCTGAGTAGC CCGTCGCTGA ATTCCGCGGT GCCGACGCCT
GCCTGTCCTT ACGCGCCGCC GACTCCTCCG TATGTTTATA GGGACACGTG TAACTCGAGC
CTGGCCAGCC TGAGACTGAA AGCAAAGCAG CACTCCAGCT TCGGCTACGC CAGCGTGCAG
AACCCGGCCT CCAACCTGAG TGCTTGCCAG TATGCAGTGG ACCGGCCCGT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17480
Accession Version XM_011532027.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 816bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pituitary homeobox 2 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_016354.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGAGACCA ACTGCCGCAA ACTGGTGTCG GCGTGTGTGC AATTAGAGAA AGATAAAAGC 
CAGCAGGGGA AGAATGAGGA CGTGGGCGCC GAGGACCCGT CTAAGAAGAA GCGGCAAAGG
CGGCAGCGGA CTCACTTTAC CAGCCAGCAG CTCCAGGAGC TGGAGGCCAC TTTCCAGAGG
AACCGCTACC CGGACATGTC CACACGCGAA GAAATCGCTG TGTGGACCAA CCTTACGGAA
GCCCGAGTCC GGGTTTGGTT CAAGAATCGT CGGGCCAAAT GGAGAAAGAG GGAGCGCAAC
CAGCAGGCCG AGCTATGCAA GAATGGCTTC GGGCCGCAGT TCAATGGGCT CATGCAGCCC
TACGACGACA TGTACCCAGG CTATTCCTAC AACAACTGGG CCGCCAAGGG CCTTACATCC
GCCTCCCTAT CCACCAAGAG CTTCCCCTTC TTCAACTCTA TGAACGTCAA CCCCCTGTCA
TCACAGAGCA TGTTTTCCCC ACCCAACTCT ATCTCGTCCA TGAGCATGTC GTCCAGCATG
GTGCCCTCAG CAGTGACAGG CGTCCCGGGC TCCAGTCTCA ACAGCCTGAA TAACTTGAAC
AACCTGAGTA GCCCGTCGCT GAATTCCGCG GTGCCGACGC CTGCCTGTCC TTACGCGCCG
CCGACTCCTC CGTATGTTTA TAGGGACACG TGTAACTCGA GCCTGGCCAG CCTGAGACTG
AAAGCAAAGC AGCACTCCAG CTTCGGCTAC GCCAGCGTGC AGAACCCGGC CTCCAACCTG
AGTGCTTGCC AGTATGCAGT GGACCGGCCC GTGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011530329.1
CDS1442..2257
Misc Feature(1)1559..1735(+)
Misc Feature(2)1559..1729(+)
Misc Feature(3)1565..1717(+)
Misc Feature(4)2123..2179(+)
Translation
Position Chain Variation Link
2 2 a, g dbSNP:138455901
5 5 c, t dbSNP:568073657
21 21 a, c dbSNP:114738786
36 36 g, t dbSNP:771605092
42 42 a, c dbSNP:768595732
91 91 c, t dbSNP:528361609
93 93 -, c dbSNP:61567593
107 107 a, c dbSNP:145638916
131 131 g, t dbSNP:192250966
138 138 a, g dbSNP:530599491
156 156 -, aa dbSNP:58693505
170 170 a, t dbSNP:774962199
183 183 -, g dbSNP:776236506
183 183 g, t dbSNP:375830124
205 205 c, g dbSNP:747895666
233 233 c, t dbSNP:563409386
291 291 c, g dbSNP:541903355
305 305 a, g dbSNP:141922822
331 331 c, g dbSNP:187210297
350 350 a, g dbSNP:754809224
374 374 c, t dbSNP:62338989
383 383 -, cttcccc dbSNP:548594714
386 386 a, c dbSNP:569838118
401 401 c, g dbSNP:2739191
405 405 c, g dbSNP:2739192
418 418 a, g dbSNP:181439547
435 435 c, t dbSNP:377371567
459 459 c, g dbSNP:2739193
462 462 c, g dbSNP:2739194
475 475 c, t dbSNP:368647502
522 522 c, t dbSNP:779178803
528 528 -, gg dbSNP:531052822
536 536 c, g dbSNP:369582630
610 610 a, c dbSNP:138899981
633 633 a, c dbSNP:569100114
649 649 a, c dbSNP:145044365
708 708 c, t dbSNP:189152290
717 717 c, t dbSNP:568128651
773 773 c, t dbSNP:546942744
778 778 a, c dbSNP:528451197
816 816 c, t dbSNP:570755690
847 847 c, t dbSNP:185557577
893 893 a, c dbSNP:13145739
922 922 g, t dbSNP:755517957
957 957 c, g dbSNP:2739200
962 962 g, t dbSNP:563434293
963 963 c, t dbSNP:548322604
973 973 c, t dbSNP:529787081
1024 1024 -, c dbSNP:111246791
1025 1025 -, c dbSNP:551766296
1035 1035 a, g dbSNP:559470704
1043 1043 a, c dbSNP:35956624
1050 1050 g, t dbSNP:113778706
1080 1080 c, g dbSNP:181197472
1082 1082 a, c dbSNP:564846436
1091 1091 a, c dbSNP:546132428
1130 1130 a, g dbSNP:776489864
1148 1148 c, t dbSNP:149097993
1155 1155 a, g dbSNP:113717851
1158 1158 -, ag dbSNP:774880258
1231 1231 a, g dbSNP:111733107
1237 1237 a, c dbSNP:535573526
1251 1251 a, g dbSNP:371965728
1303 1303 c, t dbSNP:574619224
1320 1320 g, t dbSNP:553173018
1363 1363 a, g dbSNP:535056127
1413 1413 a, g dbSNP:570712814
1422 1422 a, c dbSNP:2739203
1443 1443 g, t dbSNP:75334582
1447 1447 a, g dbSNP:376818069
1454 1454 c, g, t dbSNP:200683912
1465 1465 a, g, t dbSNP:143778014
1468 1468 a, g, t dbSNP:531925274
1469 1469 a, t dbSNP:139906074
1471 1471 c, g dbSNP:746215581
1473 1473 c, t dbSNP:373848340
1474 1474 c, g dbSNP:370048347
1477 1477 c, t dbSNP:749410556
1478 1478 c, g dbSNP:1131942
1479 1479 g, t dbSNP:374524467
1485 1485 c, t dbSNP:778111934
1495 1495 c, t dbSNP:370588233
1503 1503 a, c, t dbSNP:753935193
1504 1504 a, g dbSNP:756527247
1506 1506 a, c, g dbSNP:201628949
1514 1514 a, c dbSNP:781343571
1516 1516 a, t dbSNP:755404910
1523 1523 a, g dbSNP:535881495
1527 1527 a, g dbSNP:766774222
1530 1530 c, g dbSNP:763095137
1531 1531 c, g dbSNP:767533645
1532 1532 a, g dbSNP:765382079
1550 1550 -, aag dbSNP:770651665
1554 1554 a, c, g dbSNP:28936409
1555 1555 a, g dbSNP:149288560
1564 1564 g, t dbSNP:768386257
1580 1580 a, c dbSNP:760524995
1585 1585 c, t dbSNP:775089001
1598 1598 c, g dbSNP:1051887
1602 1602 a, t dbSNP:104893857
1626 1626 a, g dbSNP:104893862
1643 1643 a, c dbSNP:104893858
1647 1647 a, g dbSNP:104893861
1684 1684 c, t dbSNP:771757537
1688 1688 c, g dbSNP:121909249
1690 1690 c, g dbSNP:745540247
1691 1691 c, t dbSNP:121909248
1698 1698 g, t dbSNP:772800095
1703 1703 a, g dbSNP:387906810
1711 1711 c, t dbSNP:769461476
1713 1713 c, g dbSNP:104893859
1725 1725 a, g dbSNP:747454924
1726 1726 a, g dbSNP:138593200
1732 1732 a, g dbSNP:776157834
1737 1737 g, t dbSNP:772313984
1738 1738 c, t dbSNP:770354450
1744 1744 a, g dbSNP:746315869
1750 1750 a, c dbSNP:778975957
1759 1759 c, t dbSNP:757448249
1768 1768 c, t dbSNP:374404013
1770 1770 g, t dbSNP:777760630
1771 1771 c, t dbSNP:756196786
1777 1777 g, t dbSNP:752617007
1780 1780 a, g dbSNP:781170593
1785 1785 a, g dbSNP:754698054
1803 1803 a, g dbSNP:751418961
1807 1807 c, t dbSNP:113012179
1812 1812 c, t dbSNP:766110695
1817 1817 c, t dbSNP:527498517
1830 1830 a, g dbSNP:750107133
1840 1840 a, g dbSNP:104893860
1844 1844 a, g dbSNP:77144743
1849 1849 a, g dbSNP:781375019
1852 1852 c, t dbSNP:776139719
1855 1855 c, t dbSNP:772679916
1858 1858 a, t dbSNP:759978846
1862 1862 a, g dbSNP:193920830
1868 1868 c, g dbSNP:774733901
1870 1870 a, c, g dbSNP:749452484
1875 1875 c, g dbSNP:544781282
1876 1876 c, g dbSNP:577700410
1887 1887 a, c dbSNP:748266578
1892 1892 g, t dbSNP:780968713
1894 1894 c, t dbSNP:754895399
1895 1895 a, g dbSNP:751484586
1896 1896 a, g dbSNP:779833505
1900 1900 g, t dbSNP:35946364
1901 1901 a, g dbSNP:138163892
1906 1906 c, t dbSNP:765053157
1916 1916 c, t dbSNP:761378970
1921 1921 a, t dbSNP:141176394
1933 1933 c, g dbSNP:763539767
1938 1938 c, g dbSNP:760018349
1952 1952 a, g dbSNP:774730294
1953 1953 g, t dbSNP:771311013
1955 1955 c, t dbSNP:763373195
1957 1957 a, g dbSNP:140541021
1960 1960 c, t dbSNP:201319333
1963 1963 a, g dbSNP:748391268
1966 1966 c, t dbSNP:781291656
1967 1967 a, g dbSNP:768622921
1977 1977 a, g dbSNP:746908680
2002 2002 c, t dbSNP:555260580
2003 2003 a, g dbSNP:758267195
2010 2010 a, g dbSNP:146807994
2011 2011 c, t dbSNP:143714974
2020 2020 c, g dbSNP:756926743
2037 2037 g, t dbSNP:536920164
2056 2056 a, g dbSNP:763598663
2061 2061 c, t dbSNP:755635155
2062 2062 c, g dbSNP:752099801
2068 2068 c, g dbSNP:766833864
2069 2069 c, g, t dbSNP:149181425
2077 2077 g, t dbSNP:145355581
2080 2080 a, c, g dbSNP:776732876
2090 2090 a, c, t dbSNP:747126705
2091 2091 c, t dbSNP:775470219
2094 2094 a, t dbSNP:371238455
2095 2095 c, t dbSNP:745827821
2098 2098 a, g dbSNP:778779105
2099 2099 a, c dbSNP:376861814
2100 2100 c, t dbSNP:777541682
2101 2101 a, g dbSNP:148191851
2103 2103 a, c dbSNP:777353401
2115 2115 a, g dbSNP:755618053
2119 2119 a, t dbSNP:752197021
2129 2129 a, g dbSNP:374542009
2131 2131 a, g dbSNP:767036406
2144 2144 c, t dbSNP:139401187
2148 2148 c, t dbSNP:750934780
2149 2149 c, t dbSNP:573990899
2188 2188 c, g dbSNP:1051888
2189 2189 c, t dbSNP:762224214
2191 2191 c, t dbSNP:776910041
2197 2197 c, g dbSNP:764265570
2198 2198 a, g dbSNP:760790139
2206 2206 g, t dbSNP:2739199
2217 2217 a, g dbSNP:775387051
2218 2218 c, t dbSNP:772278492
2223 2223 a, g dbSNP:571758306
2232 2232 a, g dbSNP:774485351
2247 2247 c, g dbSNP:770843364
2251 2251 a, c, t dbSNP:147543001
2252 2252 c, g, t dbSNP:747865049
2260 2260 c, t dbSNP:780613951
2262 2262 a, c dbSNP:754498609
2263 2263 a, g dbSNP:750917268
2271 2271 c, t dbSNP:779465924
2272 2272 c, g dbSNP:549958385
2274 2274 c, t dbSNP:754207409
2275 2275 a, g dbSNP:764545113
2281 2281 c, g dbSNP:1051889
2288 2288 a, c dbSNP:760772767
2289 2289 g, t dbSNP:752977430
2293 2293 c, g, t dbSNP:201933531
2299 2299 a, g dbSNP:774397515
2301 2301 a, g dbSNP:771039657
2308 2308 a, c, t dbSNP:772842482
2312 2312 c, t dbSNP:750221534
2364 2364 a, c dbSNP:752553102
2370 2370 -, a dbSNP:755301025
2376 2376 a, t dbSNP:765040142
2433 2433 a, t dbSNP:567517676
2438 2438 c, t dbSNP:564793153
2465 2465 a, g dbSNP:548886681
2485 2485 c, g dbSNP:767478639
2508 2508 g, t dbSNP:527607188
2572 2572 c, t dbSNP:754978001
2582 2582 c, t dbSNP:559998304
2597 2597 a, g dbSNP:551209662
2611 2611 a, g dbSNP:761836715
2621 2621 a, g dbSNP:532938712
2631 2631 c, t dbSNP:562612539
2690 2690 a, g dbSNP:543939301
2711 2711 c, t dbSNP:6533526
2712 2712 g, t dbSNP:561697335
2728 2728 a, g dbSNP:75911264
2747 2747 c, t dbSNP:144573985
2766 2766 -, attat dbSNP:754182898
2766 2766 a, g dbSNP:554499964
2774 2774 -, tattat dbSNP:764176907
2774 2774 -, tat dbSNP:775662096
2776 2776 -, tat dbSNP:569839843
2777 2777 -, tat dbSNP:561702585
2779 2779 c, t dbSNP:188349821
2814 2814 c, g dbSNP:2739201
2816 2816 c, g dbSNP:2739202
2832 2832 c, t dbSNP:759153090
2847 2847 -, ta dbSNP:200739974
2850 2850 a, g dbSNP:760397096
2854 2854 a, g dbSNP:772929558
2888 2888 -, att dbSNP:767699594
2944 2944 a, g dbSNP:185925533

Target ORF information:

RefSeq Version XM_011532027
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens paired-like homeodomain 2 (PITX2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011532027

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGAGACCA ACTGCCGCAA ACTGGTGTCG GCGTGTGTGC AATTAGAGAA AGATAAAAGC 
CAGCAGGGGA AGAATGAGGA CGTGGGCGCC GAGGACCCGT CTAAGAAGAA GCGGCAAAGG
CGGCAGCGGA CTCACTTTAC CAGCCAGCAG CTCCAGGAGC TGGAGGCCAC TTTCCAGAGG
AACCGCTACC CGGACATGTC CACACGCGAA GAAATCGCTG TGTGGACCAA CCTTACGGAA
GCCCGAGTCC GGGTTTGGTT CAAGAATCGT CGGGCCAAAT GGAGAAAGAG GGAGCGCAAC
CAGCAGGCCG AGCTATGCAA GAATGGCTTC GGGCCGCAGT TCAATGGGCT CATGCAGCCC
TACGACGACA TGTACCCAGG CTATTCCTAC AACAACTGGG CCGCCAAGGG CCTTACATCC
GCCTCCCTAT CCACCAAGAG CTTCCCCTTC TTCAACTCTA TGAACGTCAA CCCCCTGTCA
TCACAGAGCA TGTTTTCCCC ACCCAACTCT ATCTCGTCCA TGAGCATGTC GTCCAGCATG
GTGCCCTCAG CAGTGACAGG CGTCCCGGGC TCCAGTCTCA ACAGCCTGAA TAACTTGAAC
AACCTGAGTA GCCCGTCGCT GAATTCCGCG GTGCCGACGC CTGCCTGTCC TTACGCGCCG
CCGACTCCTC CGTATGTTTA TAGGGACACG TGTAACTCGA GCCTGGCCAG CCTGAGACTG
AAAGCAAAGC AGCACTCCAG CTTCGGCTAC GCCAGCGTGC AGAACCCGGC CTCCAACCTG
AGTGCTTGCC AGTATGCAGT GGACCGGCCC GTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17508
Accession Version NM_001204397.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pituitary homeobox 2 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP372081.1, AK127829.1, BX380370.2, U69961.1 and BC106010.1. Summary: This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. The encoded protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. This protein plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdominal organs, and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this gene are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (4) has an additional exon in the 5' UTR, as compared to variant 2. Variants 2, 4 and 5 encode the same isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK127829.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2153307 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACCA ACTGCCGCAA ACTGGTGTCG GCGTGTGTGC AATTAGGCGT GCAGCCGGCG 
GCCGTTGAAT GTCTCTTCTC CAAAGACTCC GAAATCAAAA AGGTCGAGTT CACGGACTCT
CCTGAGAGCC GAAAAGAGGC AGCCAGCAGC AAGTTCTTCC CGCGGCAGCA TCCTGGCGCC
AATGAGAAAG ATAAAAGCCA GCAGGGGAAG AATGAGGACG TGGGCGCCGA GGACCCGTCT
AAGAAGAAGC GGCAAAGGCG GCAGCGGACT CACTTTACCA GCCAGCAGCT CCAGGAGCTG
GAGGCCACTT TCCAGAGGAA CCGCTACCCG GACATGTCCA CACGCGAAGA AATCGCTGTG
TGGACCAACC TTACGGAAGC CCGAGTCCGG GTTTGGTTCA AGAATCGTCG GGCCAAATGG
AGAAAGAGGG AGCGCAACCA GCAGGCCGAG CTATGCAAGA ATGGCTTCGG GCCGCAGTTC
AATGGGCTCA TGCAGCCCTA CGACGACATG TACCCAGGCT ATTCCTACAA CAACTGGGCC
GCCAAGGGCC TTACATCCGC CTCCCTATCC ACCAAGAGCT TCCCCTTCTT CAACTCTATG
AACGTCAACC CCCTGTCATC ACAGAGCATG TTTTCCCCAC CCAACTCTAT CTCGTCCATG
AGCATGTCGT CCAGCATGGT GCCCTCAGCA GTGACAGGCG TCCCGGGCTC CAGTCTCAAC
AGCCTGAATA ACTTGAACAA CCTGAGTAGC CCGTCGCTGA ATTCCGCGGT GCCGACGCCT
GCCTGTCCTT ACGCGCCGCC GACTCCTCCG TATGTTTATA GGGACACGTG TAACTCGAGC
CTGGCCAGCC TGAGACTGAA AGCAAAGCAG CACTCCAGCT TCGGCTACGC CAGCGTGCAG
AACCCGGCCT CCAACCTGAG TGCTTGCCAG TATGCAGTGG ACCGGCCCGT GTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001191326.1
CDS1824..2777
Misc Feature(1)1815..1817(+)
Misc Feature(2)2079..2255(+)
Misc Feature(3)2079..2249(+)
Misc Feature(4)2085..2237(+)
Misc Feature(5)2643..2699(+)
Misc Feature(6)2658..2699(+)
Misc Feature(7)2676..2690(+)
Exon (1)1..205
Gene:PITX2
Gene Synonym:
Exon (2)206..1579
Gene:PITX2
Gene Synonym:
Exon (3)1580..1813
Gene:PITX2
Gene Synonym:
Exon (4)1814..1869
Gene:PITX2
Gene Synonym:
Exon (5)1870..2007
Gene:PITX2
Gene Synonym:
Exon (6)2008..2213
Gene:PITX2
Gene Synonym:
Exon (7)2214..3479
Gene:PITX2
Gene Synonym:
Translation
Position Chain Variation Link
26 26 c, g dbSNP:746939015
28 28 g, t dbSNP:535584251
31 31 g, t dbSNP:549860822
58 58 a, c dbSNP:117231596
80 80 a, c dbSNP:777788477
133 133 c, t dbSNP:560563915
165 165 g, t dbSNP:568054450
169 169 c, g dbSNP:758425186
174 174 a, g dbSNP:752107218
239 239 c, t dbSNP:528361609
241 241 -, c dbSNP:61567593
255 255 a, c dbSNP:145638916
279 279 g, t dbSNP:192250966
286 286 a, g dbSNP:530599491
304 304 -, aa dbSNP:58693505
318 318 a, t dbSNP:774962199
331 331 -, g dbSNP:776236506
331 331 g, t dbSNP:375830124
353 353 c, g dbSNP:747895666
381 381 c, t dbSNP:563409386
439 439 c, g dbSNP:541903355
453 453 a, g dbSNP:141922822
479 479 c, g dbSNP:187210297
498 498 a, g dbSNP:754809224
522 522 c, t dbSNP:62338989
531 531 -, cttcccc dbSNP:548594714
534 534 a, c dbSNP:569838118
549 549 c, g dbSNP:2739191
553 553 c, g dbSNP:2739192
566 566 a, g dbSNP:181439547
583 583 c, t dbSNP:377371567
607 607 c, g dbSNP:2739193
610 610 c, g dbSNP:2739194
623 623 c, t dbSNP:368647502
670 670 c, t dbSNP:779178803
676 676 -, gg dbSNP:531052822
684 684 c, g dbSNP:369582630
758 758 a, c dbSNP:138899981
781 781 a, c dbSNP:569100114
797 797 a, c dbSNP:145044365
856 856 c, t dbSNP:189152290
865 865 c, t dbSNP:568128651
921 921 c, t dbSNP:546942744
926 926 a, c dbSNP:528451197
964 964 c, t dbSNP:570755690
995 995 c, t dbSNP:185557577
1041 1041 a, c dbSNP:13145739
1070 1070 g, t dbSNP:755517957
1105 1105 c, g dbSNP:2739200
1110 1110 g, t dbSNP:563434293
1111 1111 c, t dbSNP:548322604
1121 1121 c, t dbSNP:529787081
1172 1172 -, c dbSNP:111246791
1173 1173 -, c dbSNP:551766296
1183 1183 a, g dbSNP:559470704
1191 1191 a, c dbSNP:35956624
1198 1198 g, t dbSNP:113778706
1228 1228 c, g dbSNP:181197472
1230 1230 a, c dbSNP:564846436
1239 1239 a, c dbSNP:546132428
1278 1278 a, g dbSNP:776489864
1296 1296 c, t dbSNP:149097993
1303 1303 a, g dbSNP:113717851
1306 1306 -, ag dbSNP:774880258
1379 1379 a, g dbSNP:111733107
1385 1385 a, c dbSNP:535573526
1399 1399 a, g dbSNP:371965728
1451 1451 c, t dbSNP:574619224
1468 1468 g, t dbSNP:553173018
1511 1511 a, g dbSNP:535056127
1561 1561 a, g dbSNP:570712814
1570 1570 a, c dbSNP:2739203
1627 1627 c, t dbSNP:566884131
1689 1689 c, t dbSNP:377536294
1723 1723 c, t dbSNP:772672864
1738 1738 c, g dbSNP:530303045
1756 1756 a, g dbSNP:771863719
1763 1763 a, g dbSNP:72898110
1765 1765 a, c dbSNP:182641204
1807 1807 a, g dbSNP:541299082
1813 1813 c, g dbSNP:532731631
1825 1825 g, t dbSNP:75334582
1829 1829 a, g dbSNP:376818069
1836 1836 c, g, t dbSNP:200683912
1847 1847 a, g, t dbSNP:143778014
1850 1850 a, g, t dbSNP:531925274
1851 1851 a, t dbSNP:139906074
1853 1853 c, g dbSNP:746215581
1855 1855 c, t dbSNP:373848340
1856 1856 c, g dbSNP:370048347
1859 1859 c, t dbSNP:749410556
1860 1860 c, g dbSNP:1131942
1861 1861 g, t dbSNP:374524467
1867 1867 c, t dbSNP:778111934
1871 1871 c, g dbSNP:763636556
1872 1872 g, t dbSNP:760317329
1882 1882 c, t dbSNP:150684621
1887 1887 c, g dbSNP:771505272
1890 1890 a, g dbSNP:763204161
1903 1903 c, t dbSNP:201299310
1908 1908 g, t dbSNP:770173811
1927 1927 c, t dbSNP:748305151
1929 1929 a, g dbSNP:781408424
1935 1935 a, g dbSNP:753677423
1937 1937 g, t dbSNP:200782789
1938 1938 g, t dbSNP:779828091
1944 1944 c, g dbSNP:758414076
1957 1957 a, g dbSNP:750353276
1959 1959 c, g dbSNP:565709983
1976 1976 a, g dbSNP:374552405
1988 1988 a, g dbSNP:142015013
1994 1994 a, t dbSNP:753524953
1995 1995 c, g dbSNP:763902543
2000 2000 a, c, t dbSNP:752299951
2005 2005 a, t dbSNP:766958445
2006 2006 c, t dbSNP:763475806
2015 2015 c, t dbSNP:370588233
2023 2023 a, c, t dbSNP:753935193
2024 2024 a, g dbSNP:756527247
2026 2026 a, c, g dbSNP:201628949
2034 2034 a, c dbSNP:781343571
2036 2036 a, t dbSNP:755404910
2043 2043 a, g dbSNP:535881495
2047 2047 a, g dbSNP:766774222
2050 2050 c, g dbSNP:763095137
2051 2051 c, g dbSNP:767533645
2052 2052 a, g dbSNP:765382079
2070 2070 -, aag dbSNP:770651665
2074 2074 a, c, g dbSNP:28936409
2075 2075 a, g dbSNP:149288560
2084 2084 g, t dbSNP:768386257
2100 2100 a, c dbSNP:760524995
2105 2105 c, t dbSNP:775089001
2118 2118 c, g dbSNP:1051887
2122 2122 a, t dbSNP:104893857
2146 2146 a, g dbSNP:104893862
2163 2163 a, c dbSNP:104893858
2167 2167 a, g dbSNP:104893861
2204 2204 c, t dbSNP:771757537
2208 2208 c, g dbSNP:121909249
2210 2210 c, g dbSNP:745540247
2211 2211 c, t dbSNP:121909248
2218 2218 g, t dbSNP:772800095
2223 2223 a, g dbSNP:387906810
2231 2231 c, t dbSNP:769461476
2233 2233 c, g dbSNP:104893859
2245 2245 a, g dbSNP:747454924
2246 2246 a, g dbSNP:138593200
2252 2252 a, g dbSNP:776157834
2257 2257 g, t dbSNP:772313984
2258 2258 c, t dbSNP:770354450
2264 2264 a, g dbSNP:746315869
2270 2270 a, c dbSNP:778975957
2279 2279 c, t dbSNP:757448249
2288 2288 c, t dbSNP:374404013
2290 2290 g, t dbSNP:777760630
2291 2291 c, t dbSNP:756196786
2297 2297 g, t dbSNP:752617007
2300 2300 a, g dbSNP:781170593
2305 2305 a, g dbSNP:754698054
2323 2323 a, g dbSNP:751418961
2327 2327 c, t dbSNP:113012179
2332 2332 c, t dbSNP:766110695
2337 2337 c, t dbSNP:527498517
2350 2350 a, g dbSNP:750107133
2360 2360 a, g dbSNP:104893860
2364 2364 a, g dbSNP:77144743
2369 2369 a, g dbSNP:781375019
2372 2372 c, t dbSNP:776139719
2375 2375 c, t dbSNP:772679916
2378 2378 a, t dbSNP:759978846
2382 2382 a, g dbSNP:193920830
2388 2388 c, g dbSNP:774733901
2390 2390 a, c, g dbSNP:749452484
2395 2395 c, g dbSNP:544781282
2396 2396 c, g dbSNP:577700410
2407 2407 a, c dbSNP:748266578
2412 2412 g, t dbSNP:780968713
2414 2414 c, t dbSNP:754895399
2415 2415 a, g dbSNP:751484586
2416 2416 a, g dbSNP:779833505
2420 2420 g, t dbSNP:35946364
2421 2421 a, g dbSNP:138163892
2426 2426 c, t dbSNP:765053157
2436 2436 c, t dbSNP:761378970
2441 2441 a, t dbSNP:141176394
2453 2453 c, g dbSNP:763539767
2458 2458 c, g dbSNP:760018349
2472 2472 a, g dbSNP:774730294
2473 2473 g, t dbSNP:771311013
2475 2475 c, t dbSNP:763373195
2477 2477 a, g dbSNP:140541021
2480 2480 c, t dbSNP:201319333
2483 2483 a, g dbSNP:748391268
2486 2486 c, t dbSNP:781291656
2487 2487 a, g dbSNP:768622921
2497 2497 a, g dbSNP:746908680
2522 2522 c, t dbSNP:555260580
2523 2523 a, g dbSNP:758267195
2530 2530 a, g dbSNP:146807994
2531 2531 c, t dbSNP:143714974
2540 2540 c, g dbSNP:756926743
2557 2557 g, t dbSNP:536920164
2576 2576 a, g dbSNP:763598663
2581 2581 c, t dbSNP:755635155
2582 2582 c, g dbSNP:752099801
2588 2588 c, g dbSNP:766833864
2589 2589 c, g, t dbSNP:149181425
2597 2597 g, t dbSNP:145355581
2600 2600 a, c, g dbSNP:776732876
2610 2610 a, c, t dbSNP:747126705
2611 2611 c, t dbSNP:775470219
2614 2614 a, t dbSNP:371238455
2615 2615 c, t dbSNP:745827821
2618 2618 a, g dbSNP:778779105
2619 2619 a, c dbSNP:376861814
2620 2620 c, t dbSNP:777541682
2621 2621 a, g dbSNP:148191851
2623 2623 a, c dbSNP:777353401
2635 2635 a, g dbSNP:755618053
2639 2639 a, t dbSNP:752197021
2649 2649 a, g dbSNP:374542009
2651 2651 a, g dbSNP:767036406
2664 2664 c, t dbSNP:139401187
2668 2668 c, t dbSNP:750934780
2669 2669 c, t dbSNP:573990899
2708 2708 c, g dbSNP:1051888
2709 2709 c, t dbSNP:762224214
2711 2711 c, t dbSNP:776910041
2717 2717 c, g dbSNP:764265570
2718 2718 a, g dbSNP:760790139
2726 2726 g, t dbSNP:2739199
2737 2737 a, g dbSNP:775387051
2738 2738 c, t dbSNP:772278492
2743 2743 a, g dbSNP:571758306
2752 2752 a, g dbSNP:774485351
2767 2767 c, g dbSNP:770843364
2771 2771 a, c, t dbSNP:147543001
2772 2772 c, g, t dbSNP:747865049
2780 2780 c, t dbSNP:780613951
2782 2782 a, c dbSNP:754498609
2783 2783 a, g dbSNP:750917268
2791 2791 c, t dbSNP:779465924
2792 2792 c, g dbSNP:549958385
2794 2794 c, t dbSNP:754207409
2795 2795 a, g dbSNP:764545113
2801 2801 c, g dbSNP:1051889
2808 2808 a, c dbSNP:760772767
2809 2809 g, t dbSNP:752977430
2813 2813 c, g, t dbSNP:201933531
2819 2819 a, g dbSNP:774397515
2821 2821 a, g dbSNP:771039657
2828 2828 a, c, t dbSNP:772842482
2832 2832 c, t dbSNP:750221534
2884 2884 a, c dbSNP:752553102
2890 2890 -, a dbSNP:755301025
2896 2896 a, t dbSNP:765040142
2953 2953 a, t dbSNP:567517676
2958 2958 c, t dbSNP:564793153
2985 2985 a, g dbSNP:548886681
3005 3005 c, g dbSNP:767478639
3028 3028 g, t dbSNP:527607188
3092 3092 c, t dbSNP:754978001
3102 3102 c, t dbSNP:559998304
3117 3117 a, g dbSNP:551209662
3131 3131 a, g dbSNP:761836715
3141 3141 a, g dbSNP:532938712
3151 3151 c, t dbSNP:562612539
3210 3210 a, g dbSNP:543939301
3231 3231 c, t dbSNP:6533526
3232 3232 g, t dbSNP:561697335
3248 3248 a, g dbSNP:75911264
3267 3267 c, t dbSNP:144573985
3286 3286 -, attat dbSNP:754182898
3286 3286 a, g dbSNP:554499964
3294 3294 -, tattat dbSNP:764176907
3294 3294 -, tat dbSNP:775662096
3296 3296 -, tat dbSNP:569839843
3297 3297 -, tat dbSNP:561702585
3299 3299 c, t dbSNP:188349821
3334 3334 c, g dbSNP:2739201
3336 3336 c, g dbSNP:2739202
3352 3352 c, t dbSNP:759153090
3367 3367 -, ta dbSNP:200739974
3370 3370 a, g dbSNP:760397096
3374 3374 a, g dbSNP:772929558
3408 3408 -, att dbSNP:767699594
3464 3464 a, g dbSNP:185925533

Target ORF information:

RefSeq Version NM_001204397
Organism Homo sapiens (human)
Definition Homo sapiens paired-like homeodomain 2 (PITX2), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204397

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACCA ACTGCCGCAA ACTGGTGTCG GCGTGTGTGC AATTAGGCGT GCAGCCGGCG 
GCCGTTGAAT GTCTCTTCTC CAAAGACTCC GAAATCAAAA AGGTCGAGTT CACGGACTCT
CCTGAGAGCC GAAAAGAGGC AGCCAGCAGC AAGTTCTTCC CGCGGCAGCA TCCTGGCGCC
AATGAGAAAG ATAAAAGCCA GCAGGGGAAG AATGAGGACG TGGGCGCCGA GGACCCGTCT
AAGAAGAAGC GGCAAAGGCG GCAGCGGACT CACTTTACCA GCCAGCAGCT CCAGGAGCTG
GAGGCCACTT TCCAGAGGAA CCGCTACCCG GACATGTCCA CACGCGAAGA AATCGCTGTG
TGGACCAACC TTACGGAAGC CCGAGTCCGG GTTTGGTTCA AGAATCGTCG GGCCAAATGG
AGAAAGAGGG AGCGCAACCA GCAGGCCGAG CTATGCAAGA ATGGCTTCGG GCCGCAGTTC
AATGGGCTCA TGCAGCCCTA CGACGACATG TACCCAGGCT ATTCCTACAA CAACTGGGCC
GCCAAGGGCC TTACATCCGC CTCCCTATCC ACCAAGAGCT TCCCCTTCTT CAACTCTATG
AACGTCAACC CCCTGTCATC ACAGAGCATG TTTTCCCCAC CCAACTCTAT CTCGTCCATG
AGCATGTCGT CCAGCATGGT GCCCTCAGCA GTGACAGGCG TCCCGGGCTC CAGTCTCAAC
AGCCTGAATA ACTTGAACAA CCTGAGTAGC CCGTCGCTGA ATTCCGCGGT GCCGACGCCT
GCCTGTCCTT ACGCGCCGCC GACTCCTCCG TATGTTTATA GGGACACGTG TAACTCGAGC
CTGGCCAGCC TGAGACTGAA AGCAAAGCAG CACTCCAGCT TCGGCTACGC CAGCGTGCAG
AACCCGGCCT CCAACCTGAG TGCTTGCCAG TATGCAGTGG ACCGGCCCGT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17508
Accession Version NM_001204398.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pituitary homeobox 2 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP372081.1 and BC106010.1. Summary: This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. The encoded protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. This protein plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdominal organs, and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this gene are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (5) lacks an exon in the 5' UTR, as compared to variant 2. Variants 2, 4 and 5 encode the same isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX403546.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153307 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACCA ACTGCCGCAA ACTGGTGTCG GCGTGTGTGC AATTAGGCGT GCAGCCGGCG 
GCCGTTGAAT GTCTCTTCTC CAAAGACTCC GAAATCAAAA AGGTCGAGTT CACGGACTCT
CCTGAGAGCC GAAAAGAGGC AGCCAGCAGC AAGTTCTTCC CGCGGCAGCA TCCTGGCGCC
AATGAGAAAG ATAAAAGCCA GCAGGGGAAG AATGAGGACG TGGGCGCCGA GGACCCGTCT
AAGAAGAAGC GGCAAAGGCG GCAGCGGACT CACTTTACCA GCCAGCAGCT CCAGGAGCTG
GAGGCCACTT TCCAGAGGAA CCGCTACCCG GACATGTCCA CACGCGAAGA AATCGCTGTG
TGGACCAACC TTACGGAAGC CCGAGTCCGG GTTTGGTTCA AGAATCGTCG GGCCAAATGG
AGAAAGAGGG AGCGCAACCA GCAGGCCGAG CTATGCAAGA ATGGCTTCGG GCCGCAGTTC
AATGGGCTCA TGCAGCCCTA CGACGACATG TACCCAGGCT ATTCCTACAA CAACTGGGCC
GCCAAGGGCC TTACATCCGC CTCCCTATCC ACCAAGAGCT TCCCCTTCTT CAACTCTATG
AACGTCAACC CCCTGTCATC ACAGAGCATG TTTTCCCCAC CCAACTCTAT CTCGTCCATG
AGCATGTCGT CCAGCATGGT GCCCTCAGCA GTGACAGGCG TCCCGGGCTC CAGTCTCAAC
AGCCTGAATA ACTTGAACAA CCTGAGTAGC CCGTCGCTGA ATTCCGCGGT GCCGACGCCT
GCCTGTCCTT ACGCGCCGCC GACTCCTCCG TATGTTTATA GGGACACGTG TAACTCGAGC
CTGGCCAGCC TGAGACTGAA AGCAAAGCAG CACTCCAGCT TCGGCTACGC CAGCGTGCAG
AACCCGGCCT CCAACCTGAG TGCTTGCCAG TATGCAGTGG ACCGGCCCGT GTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001191327.1
CDS216..1169
Misc Feature(1)207..209(+)
Misc Feature(2)471..647(+)
Misc Feature(3)471..641(+)
Misc Feature(4)477..629(+)
Misc Feature(5)1035..1091(+)
Misc Feature(6)1050..1091(+)
Misc Feature(7)1068..1082(+)
Exon (1)1..205
Gene:PITX2
Gene Synonym:
Exon (2)206..261
Gene:PITX2
Gene Synonym:
Exon (3)262..399
Gene:PITX2
Gene Synonym:
Exon (4)400..605
Gene:PITX2
Gene Synonym:
Exon (5)606..1871
Gene:PITX2
Gene Synonym:
Translation
Position Chain Variation Link
26 26 c, g dbSNP:746939015
28 28 g, t dbSNP:535584251
31 31 g, t dbSNP:549860822
58 58 a, c dbSNP:117231596
80 80 a, c dbSNP:777788477
133 133 c, t dbSNP:560563915
165 165 g, t dbSNP:568054450
169 169 c, g dbSNP:758425186
174 174 a, g dbSNP:752107218
217 217 g, t dbSNP:75334582
221 221 a, g dbSNP:376818069
228 228 c, g, t dbSNP:200683912
239 239 a, g, t dbSNP:143778014
242 242 a, g, t dbSNP:531925274
243 243 a, t dbSNP:139906074
245 245 c, g dbSNP:746215581
247 247 c, t dbSNP:373848340
248 248 c, g dbSNP:370048347
251 251 c, t dbSNP:749410556
252 252 c, g dbSNP:1131942
253 253 g, t dbSNP:374524467
259 259 c, t dbSNP:778111934
263 263 c, g dbSNP:763636556
264 264 g, t dbSNP:760317329
274 274 c, t dbSNP:150684621
279 279 c, g dbSNP:771505272
282 282 a, g dbSNP:763204161
295 295 c, t dbSNP:201299310
300 300 g, t dbSNP:770173811
319 319 c, t dbSNP:748305151
321 321 a, g dbSNP:781408424
327 327 a, g dbSNP:753677423
329 329 g, t dbSNP:200782789
330 330 g, t dbSNP:779828091
336 336 c, g dbSNP:758414076
349 349 a, g dbSNP:750353276
351 351 c, g dbSNP:565709983
368 368 a, g dbSNP:374552405
380 380 a, g dbSNP:142015013
386 386 a, t dbSNP:753524953
387 387 c, g dbSNP:763902543
392 392 a, c, t dbSNP:752299951
397 397 a, t dbSNP:766958445
398 398 c, t dbSNP:763475806
407 407 c, t dbSNP:370588233
415 415 a, c, t dbSNP:753935193
416 416 a, g dbSNP:756527247
418 418 a, c, g dbSNP:201628949
426 426 a, c dbSNP:781343571
428 428 a, t dbSNP:755404910
435 435 a, g dbSNP:535881495
439 439 a, g dbSNP:766774222
442 442 c, g dbSNP:763095137
443 443 c, g dbSNP:767533645
444 444 a, g dbSNP:765382079
462 462 -, aag dbSNP:770651665
466 466 a, c, g dbSNP:28936409
467 467 a, g dbSNP:149288560
476 476 g, t dbSNP:768386257
492 492 a, c dbSNP:760524995
497 497 c, t dbSNP:775089001
510 510 c, g dbSNP:1051887
514 514 a, t dbSNP:104893857
538 538 a, g dbSNP:104893862
555 555 a, c dbSNP:104893858
559 559 a, g dbSNP:104893861
596 596 c, t dbSNP:771757537
600 600 c, g dbSNP:121909249
602 602 c, g dbSNP:745540247
603 603 c, t dbSNP:121909248
610 610 g, t dbSNP:772800095
615 615 a, g dbSNP:387906810
623 623 c, t dbSNP:769461476
625 625 c, g dbSNP:104893859
637 637 a, g dbSNP:747454924
638 638 a, g dbSNP:138593200
644 644 a, g dbSNP:776157834
649 649 g, t dbSNP:772313984
650 650 c, t dbSNP:770354450
656 656 a, g dbSNP:746315869
662 662 a, c dbSNP:778975957
671 671 c, t dbSNP:757448249
680 680 c, t dbSNP:374404013
682 682 g, t dbSNP:777760630
683 683 c, t dbSNP:756196786
689 689 g, t dbSNP:752617007
692 692 a, g dbSNP:781170593
697 697 a, g dbSNP:754698054
715 715 a, g dbSNP:751418961
719 719 c, t dbSNP:113012179
724 724 c, t dbSNP:766110695
729 729 c, t dbSNP:527498517
742 742 a, g dbSNP:750107133
752 752 a, g dbSNP:104893860
756 756 a, g dbSNP:77144743
761 761 a, g dbSNP:781375019
764 764 c, t dbSNP:776139719
767 767 c, t dbSNP:772679916
770 770 a, t dbSNP:759978846
774 774 a, g dbSNP:193920830
780 780 c, g dbSNP:774733901
782 782 a, c, g dbSNP:749452484
787 787 c, g dbSNP:544781282
788 788 c, g dbSNP:577700410
799 799 a, c dbSNP:748266578
804 804 g, t dbSNP:780968713
806 806 c, t dbSNP:754895399
807 807 a, g dbSNP:751484586
808 808 a, g dbSNP:779833505
812 812 g, t dbSNP:35946364
813 813 a, g dbSNP:138163892
818 818 c, t dbSNP:765053157
828 828 c, t dbSNP:761378970
833 833 a, t dbSNP:141176394
845 845 c, g dbSNP:763539767
850 850 c, g dbSNP:760018349
864 864 a, g dbSNP:774730294
865 865 g, t dbSNP:771311013
867 867 c, t dbSNP:763373195
869 869 a, g dbSNP:140541021
872 872 c, t dbSNP:201319333
875 875 a, g dbSNP:748391268
878 878 c, t dbSNP:781291656
879 879 a, g dbSNP:768622921
889 889 a, g dbSNP:746908680
914 914 c, t dbSNP:555260580
915 915 a, g dbSNP:758267195
922 922 a, g dbSNP:146807994
923 923 c, t dbSNP:143714974
932 932 c, g dbSNP:756926743
949 949 g, t dbSNP:536920164
968 968 a, g dbSNP:763598663
973 973 c, t dbSNP:755635155
974 974 c, g dbSNP:752099801
980 980 c, g dbSNP:766833864
981 981 c, g, t dbSNP:149181425
989 989 g, t dbSNP:145355581
992 992 a, c, g dbSNP:776732876
1002 1002 a, c, t dbSNP:747126705
1003 1003 c, t dbSNP:775470219
1006 1006 a, t dbSNP:371238455
1007 1007 c, t dbSNP:745827821
1010 1010 a, g dbSNP:778779105
1011 1011 a, c dbSNP:376861814
1012 1012 c, t dbSNP:777541682
1013 1013 a, g dbSNP:148191851
1015 1015 a, c dbSNP:777353401
1027 1027 a, g dbSNP:755618053
1031 1031 a, t dbSNP:752197021
1041 1041 a, g dbSNP:374542009
1043 1043 a, g dbSNP:767036406
1056 1056 c, t dbSNP:139401187
1060 1060 c, t dbSNP:750934780
1061 1061 c, t dbSNP:573990899
1100 1100 c, g dbSNP:1051888
1101 1101 c, t dbSNP:762224214
1103 1103 c, t dbSNP:776910041
1109 1109 c, g dbSNP:764265570
1110 1110 a, g dbSNP:760790139
1118 1118 g, t dbSNP:2739199
1129 1129 a, g dbSNP:775387051
1130 1130 c, t dbSNP:772278492
1135 1135 a, g dbSNP:571758306
1144 1144 a, g dbSNP:774485351
1159 1159 c, g dbSNP:770843364
1163 1163 a, c, t dbSNP:147543001
1164 1164 c, g, t dbSNP:747865049
1172 1172 c, t dbSNP:780613951
1174 1174 a, c dbSNP:754498609
1175 1175 a, g dbSNP:750917268
1183 1183 c, t dbSNP:779465924
1184 1184 c, g dbSNP:549958385
1186 1186 c, t dbSNP:754207409
1187 1187 a, g dbSNP:764545113
1193 1193 c, g dbSNP:1051889
1200 1200 a, c dbSNP:760772767
1201 1201 g, t dbSNP:752977430
1205 1205 c, g, t dbSNP:201933531
1211 1211 a, g dbSNP:774397515
1213 1213 a, g dbSNP:771039657
1220 1220 a, c, t dbSNP:772842482
1224 1224 c, t dbSNP:750221534
1276 1276 a, c dbSNP:752553102
1282 1282 -, a dbSNP:755301025
1288 1288 a, t dbSNP:765040142
1345 1345 a, t dbSNP:567517676
1350 1350 c, t dbSNP:564793153
1377 1377 a, g dbSNP:548886681
1397 1397 c, g dbSNP:767478639
1420 1420 g, t dbSNP:527607188
1484 1484 c, t dbSNP:754978001
1494 1494 c, t dbSNP:559998304
1509 1509 a, g dbSNP:551209662
1523 1523 a, g dbSNP:761836715
1533 1533 a, g dbSNP:532938712
1543 1543 c, t dbSNP:562612539
1602 1602 a, g dbSNP:543939301
1623 1623 c, t dbSNP:6533526
1624 1624 g, t dbSNP:561697335
1640 1640 a, g dbSNP:75911264
1659 1659 c, t dbSNP:144573985
1678 1678 -, attat dbSNP:754182898
1678 1678 a, g dbSNP:554499964
1686 1686 -, tattat dbSNP:764176907
1686 1686 -, tat dbSNP:775662096
1688 1688 -, tat dbSNP:569839843
1689 1689 -, tat dbSNP:561702585
1691 1691 c, t dbSNP:188349821
1726 1726 c, g dbSNP:2739201
1728 1728 c, g dbSNP:2739202
1744 1744 c, t dbSNP:759153090
1759 1759 -, ta dbSNP:200739974
1762 1762 a, g dbSNP:760397096
1766 1766 a, g dbSNP:772929558
1800 1800 -, att dbSNP:767699594
1856 1856 a, g dbSNP:185925533

Target ORF information:

RefSeq Version NM_001204398
Organism Homo sapiens (human)
Definition Homo sapiens paired-like homeodomain 2 (PITX2), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204398

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACCA ACTGCCGCAA ACTGGTGTCG GCGTGTGTGC AATTAGGCGT GCAGCCGGCG 
GCCGTTGAAT GTCTCTTCTC CAAAGACTCC GAAATCAAAA AGGTCGAGTT CACGGACTCT
CCTGAGAGCC GAAAAGAGGC AGCCAGCAGC AAGTTCTTCC CGCGGCAGCA TCCTGGCGCC
AATGAGAAAG ATAAAAGCCA GCAGGGGAAG AATGAGGACG TGGGCGCCGA GGACCCGTCT
AAGAAGAAGC GGCAAAGGCG GCAGCGGACT CACTTTACCA GCCAGCAGCT CCAGGAGCTG
GAGGCCACTT TCCAGAGGAA CCGCTACCCG GACATGTCCA CACGCGAAGA AATCGCTGTG
TGGACCAACC TTACGGAAGC CCGAGTCCGG GTTTGGTTCA AGAATCGTCG GGCCAAATGG
AGAAAGAGGG AGCGCAACCA GCAGGCCGAG CTATGCAAGA ATGGCTTCGG GCCGCAGTTC
AATGGGCTCA TGCAGCCCTA CGACGACATG TACCCAGGCT ATTCCTACAA CAACTGGGCC
GCCAAGGGCC TTACATCCGC CTCCCTATCC ACCAAGAGCT TCCCCTTCTT CAACTCTATG
AACGTCAACC CCCTGTCATC ACAGAGCATG TTTTCCCCAC CCAACTCTAT CTCGTCCATG
AGCATGTCGT CCAGCATGGT GCCCTCAGCA GTGACAGGCG TCCCGGGCTC CAGTCTCAAC
AGCCTGAATA ACTTGAACAA CCTGAGTAGC CCGTCGCTGA ATTCCGCGGT GCCGACGCCT
GCCTGTCCTT ACGCGCCGCC GACTCCTCCG TATGTTTATA GGGACACGTG TAACTCGAGC
CTGGCCAGCC TGAGACTGAA AGCAAAGCAG CACTCCAGCT TCGGCTACGC CAGCGTGCAG
AACCCGGCCT CCAACCTGAG TGCTTGCCAG TATGCAGTGG ACCGGCCCGT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17480
Accession Version NM_001204399.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 816bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pituitary homeobox 2 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP372081.1 and BC106010.1. Summary: This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. The encoded protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. This protein plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdominal organs, and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this gene are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (6) lacks an exon in the 5' UTR and an in-frame exon in the 5' CDS, as compared to variant 2. The resulting isoform (a) lacks an internal segment, as compared to isoform b. Variants 1 and 6 encode the same isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM706464.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2149398 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGAGACCA ACTGCCGCAA ACTGGTGTCG GCGTGTGTGC AATTAGAGAA AGATAAAAGC 
CAGCAGGGGA AGAATGAGGA CGTGGGCGCC GAGGACCCGT CTAAGAAGAA GCGGCAAAGG
CGGCAGCGGA CTCACTTTAC CAGCCAGCAG CTCCAGGAGC TGGAGGCCAC TTTCCAGAGG
AACCGCTACC CGGACATGTC CACACGCGAA GAAATCGCTG TGTGGACCAA CCTTACGGAA
GCCCGAGTCC GGGTTTGGTT CAAGAATCGT CGGGCCAAAT GGAGAAAGAG GGAGCGCAAC
CAGCAGGCCG AGCTATGCAA GAATGGCTTC GGGCCGCAGT TCAATGGGCT CATGCAGCCC
TACGACGACA TGTACCCAGG CTATTCCTAC AACAACTGGG CCGCCAAGGG CCTTACATCC
GCCTCCCTAT CCACCAAGAG CTTCCCCTTC TTCAACTCTA TGAACGTCAA CCCCCTGTCA
TCACAGAGCA TGTTTTCCCC ACCCAACTCT ATCTCGTCCA TGAGCATGTC GTCCAGCATG
GTGCCCTCAG CAGTGACAGG CGTCCCGGGC TCCAGTCTCA ACAGCCTGAA TAACTTGAAC
AACCTGAGTA GCCCGTCGCT GAATTCCGCG GTGCCGACGC CTGCCTGTCC TTACGCGCCG
CCGACTCCTC CGTATGTTTA TAGGGACACG TGTAACTCGA GCCTGGCCAG CCTGAGACTG
AAAGCAAAGC AGCACTCCAG CTTCGGCTAC GCCAGCGTGC AGAACCCGGC CTCCAACCTG
AGTGCTTGCC AGTATGCAGT GGACCGGCCC GTGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001191328.1
CDS216..1031
Misc Feature(1)207..209(+)
Misc Feature(2)333..509(+)
Misc Feature(3)333..503(+)
Misc Feature(4)339..491(+)
Misc Feature(5)897..953(+)
Exon (1)1..205
Gene:PITX2
Gene Synonym:
Exon (2)206..261
Gene:PITX2
Gene Synonym:
Exon (3)262..467
Gene:PITX2
Gene Synonym:
Exon (4)468..1733
Gene:PITX2
Gene Synonym:
Translation
Position Chain Variation Link
26 26 c, g dbSNP:746939015
28 28 g, t dbSNP:535584251
31 31 g, t dbSNP:549860822
58 58 a, c dbSNP:117231596
80 80 a, c dbSNP:777788477
133 133 c, t dbSNP:560563915
165 165 g, t dbSNP:568054450
169 169 c, g dbSNP:758425186
174 174 a, g dbSNP:752107218
217 217 g, t dbSNP:75334582
221 221 a, g dbSNP:376818069
228 228 c, g, t dbSNP:200683912
239 239 a, g, t dbSNP:143778014
242 242 a, g, t dbSNP:531925274
243 243 a, t dbSNP:139906074
245 245 c, g dbSNP:746215581
247 247 c, t dbSNP:373848340
248 248 c, g dbSNP:370048347
251 251 c, t dbSNP:749410556
252 252 c, g dbSNP:1131942
253 253 g, t dbSNP:374524467
259 259 c, t dbSNP:778111934
269 269 c, t dbSNP:370588233
277 277 a, c, t dbSNP:753935193
278 278 a, g dbSNP:756527247
280 280 a, c, g dbSNP:201628949
288 288 a, c dbSNP:781343571
290 290 a, t dbSNP:755404910
297 297 a, g dbSNP:535881495
301 301 a, g dbSNP:766774222
304 304 c, g dbSNP:763095137
305 305 c, g dbSNP:767533645
306 306 a, g dbSNP:765382079
324 324 -, aag dbSNP:770651665
328 328 a, c, g dbSNP:28936409
329 329 a, g dbSNP:149288560
338 338 g, t dbSNP:768386257
354 354 a, c dbSNP:760524995
359 359 c, t dbSNP:775089001
372 372 c, g dbSNP:1051887
376 376 a, t dbSNP:104893857
400 400 a, g dbSNP:104893862
417 417 a, c dbSNP:104893858
421 421 a, g dbSNP:104893861
458 458 c, t dbSNP:771757537
462 462 c, g dbSNP:121909249
464 464 c, g dbSNP:745540247
465 465 c, t dbSNP:121909248
472 472 g, t dbSNP:772800095
477 477 a, g dbSNP:387906810
485 485 c, t dbSNP:769461476
487 487 c, g dbSNP:104893859
499 499 a, g dbSNP:747454924
500 500 a, g dbSNP:138593200
506 506 a, g dbSNP:776157834
511 511 g, t dbSNP:772313984
512 512 c, t dbSNP:770354450
518 518 a, g dbSNP:746315869
524 524 a, c dbSNP:778975957
533 533 c, t dbSNP:757448249
542 542 c, t dbSNP:374404013
544 544 g, t dbSNP:777760630
545 545 c, t dbSNP:756196786
551 551 g, t dbSNP:752617007
554 554 a, g dbSNP:781170593
559 559 a, g dbSNP:754698054
577 577 a, g dbSNP:751418961
581 581 c, t dbSNP:113012179
586 586 c, t dbSNP:766110695
591 591 c, t dbSNP:527498517
604 604 a, g dbSNP:750107133
614 614 a, g dbSNP:104893860
618 618 a, g dbSNP:77144743
623 623 a, g dbSNP:781375019
626 626 c, t dbSNP:776139719
629 629 c, t dbSNP:772679916
632 632 a, t dbSNP:759978846
636 636 a, g dbSNP:193920830
642 642 c, g dbSNP:774733901
644 644 a, c, g dbSNP:749452484
649 649 c, g dbSNP:544781282
650 650 c, g dbSNP:577700410
661 661 a, c dbSNP:748266578
666 666 g, t dbSNP:780968713
668 668 c, t dbSNP:754895399
669 669 a, g dbSNP:751484586
670 670 a, g dbSNP:779833505
674 674 g, t dbSNP:35946364
675 675 a, g dbSNP:138163892
680 680 c, t dbSNP:765053157
690 690 c, t dbSNP:761378970
695 695 a, t dbSNP:141176394
707 707 c, g dbSNP:763539767
712 712 c, g dbSNP:760018349
726 726 a, g dbSNP:774730294
727 727 g, t dbSNP:771311013
729 729 c, t dbSNP:763373195
731 731 a, g dbSNP:140541021
734 734 c, t dbSNP:201319333
737 737 a, g dbSNP:748391268
740 740 c, t dbSNP:781291656
741 741 a, g dbSNP:768622921
751 751 a, g dbSNP:746908680
776 776 c, t dbSNP:555260580
777 777 a, g dbSNP:758267195
784 784 a, g dbSNP:146807994
785 785 c, t dbSNP:143714974
794 794 c, g dbSNP:756926743
811 811 g, t dbSNP:536920164
830 830 a, g dbSNP:763598663
835 835 c, t dbSNP:755635155
836 836 c, g dbSNP:752099801
842 842 c, g dbSNP:766833864
843 843 c, g, t dbSNP:149181425
851 851 g, t dbSNP:145355581
854 854 a, c, g dbSNP:776732876
864 864 a, c, t dbSNP:747126705
865 865 c, t dbSNP:775470219
868 868 a, t dbSNP:371238455
869 869 c, t dbSNP:745827821
872 872 a, g dbSNP:778779105
873 873 a, c dbSNP:376861814
874 874 c, t dbSNP:777541682
875 875 a, g dbSNP:148191851
877 877 a, c dbSNP:777353401
889 889 a, g dbSNP:755618053
893 893 a, t dbSNP:752197021
903 903 a, g dbSNP:374542009
905 905 a, g dbSNP:767036406
918 918 c, t dbSNP:139401187
922 922 c, t dbSNP:750934780
923 923 c, t dbSNP:573990899
962 962 c, g dbSNP:1051888
963 963 c, t dbSNP:762224214
965 965 c, t dbSNP:776910041
971 971 c, g dbSNP:764265570
972 972 a, g dbSNP:760790139
980 980 g, t dbSNP:2739199
991 991 a, g dbSNP:775387051
992 992 c, t dbSNP:772278492
997 997 a, g dbSNP:571758306
1006 1006 a, g dbSNP:774485351
1021 1021 c, g dbSNP:770843364
1025 1025 a, c, t dbSNP:147543001
1026 1026 c, g, t dbSNP:747865049
1034 1034 c, t dbSNP:780613951
1036 1036 a, c dbSNP:754498609
1037 1037 a, g dbSNP:750917268
1045 1045 c, t dbSNP:779465924
1046 1046 c, g dbSNP:549958385
1048 1048 c, t dbSNP:754207409
1049 1049 a, g dbSNP:764545113
1055 1055 c, g dbSNP:1051889
1062 1062 a, c dbSNP:760772767
1063 1063 g, t dbSNP:752977430
1067 1067 c, g, t dbSNP:201933531
1073 1073 a, g dbSNP:774397515
1075 1075 a, g dbSNP:771039657
1082 1082 a, c, t dbSNP:772842482
1086 1086 c, t dbSNP:750221534
1138 1138 a, c dbSNP:752553102
1144 1144 -, a dbSNP:755301025
1150 1150 a, t dbSNP:765040142
1207 1207 a, t dbSNP:567517676
1212 1212 c, t dbSNP:564793153
1239 1239 a, g dbSNP:548886681
1259 1259 c, g dbSNP:767478639
1282 1282 g, t dbSNP:527607188
1346 1346 c, t dbSNP:754978001
1356 1356 c, t dbSNP:559998304
1371 1371 a, g dbSNP:551209662
1385 1385 a, g dbSNP:761836715
1395 1395 a, g dbSNP:532938712
1405 1405 c, t dbSNP:562612539
1464 1464 a, g dbSNP:543939301
1485 1485 c, t dbSNP:6533526
1486 1486 g, t dbSNP:561697335
1502 1502 a, g dbSNP:75911264
1521 1521 c, t dbSNP:144573985
1540 1540 -, attat dbSNP:754182898
1540 1540 a, g dbSNP:554499964
1548 1548 -, tattat dbSNP:764176907
1548 1548 -, tat dbSNP:775662096
1550 1550 -, tat dbSNP:569839843
1551 1551 -, tat dbSNP:561702585
1553 1553 c, t dbSNP:188349821
1588 1588 c, g dbSNP:2739201
1590 1590 c, g dbSNP:2739202
1606 1606 c, t dbSNP:759153090
1621 1621 -, ta dbSNP:200739974
1624 1624 a, g dbSNP:760397096
1628 1628 a, g dbSNP:772929558
1662 1662 -, att dbSNP:767699594
1718 1718 a, g dbSNP:185925533

Target ORF information:

RefSeq Version NM_001204399
Organism Homo sapiens (human)
Definition Homo sapiens paired-like homeodomain 2 (PITX2), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204399

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGAGACCA ACTGCCGCAA ACTGGTGTCG GCGTGTGTGC AATTAGAGAA AGATAAAAGC 
CAGCAGGGGA AGAATGAGGA CGTGGGCGCC GAGGACCCGT CTAAGAAGAA GCGGCAAAGG
CGGCAGCGGA CTCACTTTAC CAGCCAGCAG CTCCAGGAGC TGGAGGCCAC TTTCCAGAGG
AACCGCTACC CGGACATGTC CACACGCGAA GAAATCGCTG TGTGGACCAA CCTTACGGAA
GCCCGAGTCC GGGTTTGGTT CAAGAATCGT CGGGCCAAAT GGAGAAAGAG GGAGCGCAAC
CAGCAGGCCG AGCTATGCAA GAATGGCTTC GGGCCGCAGT TCAATGGGCT CATGCAGCCC
TACGACGACA TGTACCCAGG CTATTCCTAC AACAACTGGG CCGCCAAGGG CCTTACATCC
GCCTCCCTAT CCACCAAGAG CTTCCCCTTC TTCAACTCTA TGAACGTCAA CCCCCTGTCA
TCACAGAGCA TGTTTTCCCC ACCCAACTCT ATCTCGTCCA TGAGCATGTC GTCCAGCATG
GTGCCCTCAG CAGTGACAGG CGTCCCGGGC TCCAGTCTCA ACAGCCTGAA TAACTTGAAC
AACCTGAGTA GCCCGTCGCT GAATTCCGCG GTGCCGACGC CTGCCTGTCC TTACGCGCCG
CCGACTCCTC CGTATGTTTA TAGGGACACG TGTAACTCGA GCCTGGCCAG CCTGAGACTG
AAAGCAAAGC AGCACTCCAG CTTCGGCTAC GCCAGCGTGC AGAACCCGGC CTCCAACCTG
AGTGCTTGCC AGTATGCAGT GGACCGGCCC GTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17508
Accession Version NM_153426.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pituitary homeobox 2 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP372081.1, AK127829.1, BX380370.2, U69961.1, BC106010.1 and BC013998.2. On or before Aug 31, 2013 this sequence version replaced gi:530377830, gi:24234707. Summary: This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. The encoded protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. This protein plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdominal organs, and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this gene are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (2), also known as ARP1b, encodes the predominant isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF048721.1, AK127829.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACCA ACTGCCGCAA ACTGGTGTCG GCGTGTGTGC AATTAGGCGT GCAGCCGGCG 
GCCGTTGAAT GTCTCTTCTC CAAAGACTCC GAAATCAAAA AGGTCGAGTT CACGGACTCT
CCTGAGAGCC GAAAAGAGGC AGCCAGCAGC AAGTTCTTCC CGCGGCAGCA TCCTGGCGCC
AATGAGAAAG ATAAAAGCCA GCAGGGGAAG AATGAGGACG TGGGCGCCGA GGACCCGTCT
AAGAAGAAGC GGCAAAGGCG GCAGCGGACT CACTTTACCA GCCAGCAGCT CCAGGAGCTG
GAGGCCACTT TCCAGAGGAA CCGCTACCCG GACATGTCCA CACGCGAAGA AATCGCTGTG
TGGACCAACC TTACGGAAGC CCGAGTCCGG GTTTGGTTCA AGAATCGTCG GGCCAAATGG
AGAAAGAGGG AGCGCAACCA GCAGGCCGAG CTATGCAAGA ATGGCTTCGG GCCGCAGTTC
AATGGGCTCA TGCAGCCCTA CGACGACATG TACCCAGGCT ATTCCTACAA CAACTGGGCC
GCCAAGGGCC TTACATCCGC CTCCCTATCC ACCAAGAGCT TCCCCTTCTT CAACTCTATG
AACGTCAACC CCCTGTCATC ACAGAGCATG TTTTCCCCAC CCAACTCTAT CTCGTCCATG
AGCATGTCGT CCAGCATGGT GCCCTCAGCA GTGACAGGCG TCCCGGGCTC CAGTCTCAAC
AGCCTGAATA ACTTGAACAA CCTGAGTAGC CCGTCGCTGA ATTCCGCGGT GCCGACGCCT
GCCTGTCCTT ACGCGCCGCC GACTCCTCCG TATGTTTATA GGGACACGTG TAACTCGAGC
CTGGCCAGCC TGAGACTGAA AGCAAAGCAG CACTCCAGCT TCGGCTACGC CAGCGTGCAG
AACCCGGCCT CCAACCTGAG TGCTTGCCAG TATGCAGTGG ACCGGCCCGT GTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_700475.1
CDS1590..2543
Misc Feature(1)1581..1583(+)
Misc Feature(2)1845..2021(+)
Misc Feature(3)1845..2015(+)
Misc Feature(4)1851..2003(+)
Misc Feature(5)2409..2465(+)
Misc Feature(6)2424..2465(+)
Misc Feature(7)2442..2456(+)
Exon (1)1..205
Gene:PITX2
Gene Synonym:
Exon (2)206..1579
Gene:PITX2
Gene Synonym:
Exon (3)1580..1635
Gene:PITX2
Gene Synonym:
Exon (4)1636..1773
Gene:PITX2
Gene Synonym:
Exon (5)1774..1979
Gene:PITX2
Gene Synonym:
Exon (6)1980..3245
Gene:PITX2
Gene Synonym:
Translation
Position Chain Variation Link
26 26 c, g dbSNP:746939015
28 28 g, t dbSNP:535584251
31 31 g, t dbSNP:549860822
58 58 a, c dbSNP:117231596
80 80 a, c dbSNP:777788477
133 133 c, t dbSNP:560563915
165 165 g, t dbSNP:568054450
169 169 c, g dbSNP:758425186
174 174 a, g dbSNP:752107218
239 239 c, t dbSNP:528361609
241 241 -, c dbSNP:61567593
255 255 a, c dbSNP:145638916
279 279 g, t dbSNP:192250966
286 286 a, g dbSNP:530599491
304 304 -, aa dbSNP:58693505
318 318 a, t dbSNP:774962199
331 331 -, g dbSNP:776236506
331 331 g, t dbSNP:375830124
353 353 c, g dbSNP:747895666
381 381 c, t dbSNP:563409386
439 439 c, g dbSNP:541903355
453 453 a, g dbSNP:141922822
479 479 c, g dbSNP:187210297
498 498 a, g dbSNP:754809224
522 522 c, t dbSNP:62338989
531 531 -, cttcccc dbSNP:548594714
534 534 a, c dbSNP:569838118
549 549 c, g dbSNP:2739191
553 553 c, g dbSNP:2739192
566 566 a, g dbSNP:181439547
583 583 c, t dbSNP:377371567
607 607 c, g dbSNP:2739193
610 610 c, g dbSNP:2739194
623 623 c, t dbSNP:368647502
670 670 c, t dbSNP:779178803
676 676 -, gg dbSNP:531052822
684 684 c, g dbSNP:369582630
758 758 a, c dbSNP:138899981
781 781 a, c dbSNP:569100114
797 797 a, c dbSNP:145044365
856 856 c, t dbSNP:189152290
865 865 c, t dbSNP:568128651
921 921 c, t dbSNP:546942744
926 926 a, c dbSNP:528451197
964 964 c, t dbSNP:570755690
995 995 c, t dbSNP:185557577
1041 1041 a, c dbSNP:13145739
1070 1070 g, t dbSNP:755517957
1105 1105 c, g dbSNP:2739200
1110 1110 g, t dbSNP:563434293
1111 1111 c, t dbSNP:548322604
1121 1121 c, t dbSNP:529787081
1172 1172 -, c dbSNP:111246791
1173 1173 -, c dbSNP:551766296
1183 1183 a, g dbSNP:559470704
1191 1191 a, c dbSNP:35956624
1198 1198 g, t dbSNP:113778706
1228 1228 c, g dbSNP:181197472
1230 1230 a, c dbSNP:564846436
1239 1239 a, c dbSNP:546132428
1278 1278 a, g dbSNP:776489864
1296 1296 c, t dbSNP:149097993
1303 1303 a, g dbSNP:113717851
1306 1306 -, ag dbSNP:774880258
1379 1379 a, g dbSNP:111733107
1385 1385 a, c dbSNP:535573526
1399 1399 a, g dbSNP:371965728
1451 1451 c, t dbSNP:574619224
1468 1468 g, t dbSNP:553173018
1511 1511 a, g dbSNP:535056127
1561 1561 a, g dbSNP:570712814
1570 1570 a, c dbSNP:2739203
1591 1591 g, t dbSNP:75334582
1595 1595 a, g dbSNP:376818069
1602 1602 c, g, t dbSNP:200683912
1613 1613 a, g, t dbSNP:143778014
1616 1616 a, g, t dbSNP:531925274
1617 1617 a, t dbSNP:139906074
1619 1619 c, g dbSNP:746215581
1621 1621 c, t dbSNP:373848340
1622 1622 c, g dbSNP:370048347
1625 1625 c, t dbSNP:749410556
1626 1626 c, g dbSNP:1131942
1627 1627 g, t dbSNP:374524467
1633 1633 c, t dbSNP:778111934
1637 1637 c, g dbSNP:763636556
1638 1638 g, t dbSNP:760317329
1648 1648 c, t dbSNP:150684621
1653 1653 c, g dbSNP:771505272
1656 1656 a, g dbSNP:763204161
1669 1669 c, t dbSNP:201299310
1674 1674 g, t dbSNP:770173811
1693 1693 c, t dbSNP:748305151
1695 1695 a, g dbSNP:781408424
1701 1701 a, g dbSNP:753677423
1703 1703 g, t dbSNP:200782789
1704 1704 g, t dbSNP:779828091
1710 1710 c, g dbSNP:758414076
1723 1723 a, g dbSNP:750353276
1725 1725 c, g dbSNP:565709983
1742 1742 a, g dbSNP:374552405
1754 1754 a, g dbSNP:142015013
1760 1760 a, t dbSNP:753524953
1761 1761 c, g dbSNP:763902543
1766 1766 a, c, t dbSNP:752299951
1771 1771 a, t dbSNP:766958445
1772 1772 c, t dbSNP:763475806
1781 1781 c, t dbSNP:370588233
1789 1789 a, c, t dbSNP:753935193
1790 1790 a, g dbSNP:756527247
1792 1792 a, c, g dbSNP:201628949
1800 1800 a, c dbSNP:781343571
1802 1802 a, t dbSNP:755404910
1809 1809 a, g dbSNP:535881495
1813 1813 a, g dbSNP:766774222
1816 1816 c, g dbSNP:763095137
1817 1817 c, g dbSNP:767533645
1818 1818 a, g dbSNP:765382079
1836 1836 -, aag dbSNP:770651665
1840 1840 a, c, g dbSNP:28936409
1841 1841 a, g dbSNP:149288560
1850 1850 g, t dbSNP:768386257
1866 1866 a, c dbSNP:760524995
1871 1871 c, t dbSNP:775089001
1884 1884 c, g dbSNP:1051887
1888 1888 a, t dbSNP:104893857
1912 1912 a, g dbSNP:104893862
1929 1929 a, c dbSNP:104893858
1933 1933 a, g dbSNP:104893861
1970 1970 c, t dbSNP:771757537
1974 1974 c, g dbSNP:121909249
1976 1976 c, g dbSNP:745540247
1977 1977 c, t dbSNP:121909248
1984 1984 g, t dbSNP:772800095
1989 1989 a, g dbSNP:387906810
1997 1997 c, t dbSNP:769461476
1999 1999 c, g dbSNP:104893859
2011 2011 a, g dbSNP:747454924
2012 2012 a, g dbSNP:138593200
2018 2018 a, g dbSNP:776157834
2023 2023 g, t dbSNP:772313984
2024 2024 c, t dbSNP:770354450
2030 2030 a, g dbSNP:746315869
2036 2036 a, c dbSNP:778975957
2045 2045 c, t dbSNP:757448249
2054 2054 c, t dbSNP:374404013
2056 2056 g, t dbSNP:777760630
2057 2057 c, t dbSNP:756196786
2063 2063 g, t dbSNP:752617007
2066 2066 a, g dbSNP:781170593
2071 2071 a, g dbSNP:754698054
2089 2089 a, g dbSNP:751418961
2093 2093 c, t dbSNP:113012179
2098 2098 c, t dbSNP:766110695
2103 2103 c, t dbSNP:527498517
2116 2116 a, g dbSNP:750107133
2126 2126 a, g dbSNP:104893860
2130 2130 a, g dbSNP:77144743
2135 2135 a, g dbSNP:781375019
2138 2138 c, t dbSNP:776139719
2141 2141 c, t dbSNP:772679916
2144 2144 a, t dbSNP:759978846
2148 2148 a, g dbSNP:193920830
2154 2154 c, g dbSNP:774733901
2156 2156 a, c, g dbSNP:749452484
2161 2161 c, g dbSNP:544781282
2162 2162 c, g dbSNP:577700410
2173 2173 a, c dbSNP:748266578
2178 2178 g, t dbSNP:780968713
2180 2180 c, t dbSNP:754895399
2181 2181 a, g dbSNP:751484586
2182 2182 a, g dbSNP:779833505
2186 2186 g, t dbSNP:35946364
2187 2187 a, g dbSNP:138163892
2192 2192 c, t dbSNP:765053157
2202 2202 c, t dbSNP:761378970
2207 2207 a, t dbSNP:141176394
2219 2219 c, g dbSNP:763539767
2224 2224 c, g dbSNP:760018349
2238 2238 a, g dbSNP:774730294
2239 2239 g, t dbSNP:771311013
2241 2241 c, t dbSNP:763373195
2243 2243 a, g dbSNP:140541021
2246 2246 c, t dbSNP:201319333
2249 2249 a, g dbSNP:748391268
2252 2252 c, t dbSNP:781291656
2253 2253 a, g dbSNP:768622921
2263 2263 a, g dbSNP:746908680
2288 2288 c, t dbSNP:555260580
2289 2289 a, g dbSNP:758267195
2296 2296 a, g dbSNP:146807994
2297 2297 c, t dbSNP:143714974
2306 2306 c, g dbSNP:756926743
2323 2323 g, t dbSNP:536920164
2342 2342 a, g dbSNP:763598663
2347 2347 c, t dbSNP:755635155
2348 2348 c, g dbSNP:752099801
2354 2354 c, g dbSNP:766833864
2355 2355 c, g, t dbSNP:149181425
2363 2363 g, t dbSNP:145355581
2366 2366 a, c, g dbSNP:776732876
2376 2376 a, c, t dbSNP:747126705
2377 2377 c, t dbSNP:775470219
2380 2380 a, t dbSNP:371238455
2381 2381 c, t dbSNP:745827821
2384 2384 a, g dbSNP:778779105
2385 2385 a, c dbSNP:376861814
2386 2386 c, t dbSNP:777541682
2387 2387 a, g dbSNP:148191851
2389 2389 a, c dbSNP:777353401
2401 2401 a, g dbSNP:755618053
2405 2405 a, t dbSNP:752197021
2415 2415 a, g dbSNP:374542009
2417 2417 a, g dbSNP:767036406
2430 2430 c, t dbSNP:139401187
2434 2434 c, t dbSNP:750934780
2435 2435 c, t dbSNP:573990899
2474 2474 c, g dbSNP:1051888
2475 2475 c, t dbSNP:762224214
2477 2477 c, t dbSNP:776910041
2483 2483 c, g dbSNP:764265570
2484 2484 a, g dbSNP:760790139
2492 2492 g, t dbSNP:2739199
2503 2503 a, g dbSNP:775387051
2504 2504 c, t dbSNP:772278492
2509 2509 a, g dbSNP:571758306
2518 2518 a, g dbSNP:774485351
2533 2533 c, g dbSNP:770843364
2537 2537 a, c, t dbSNP:147543001
2538 2538 c, g, t dbSNP:747865049
2546 2546 c, t dbSNP:780613951
2548 2548 a, c dbSNP:754498609
2549 2549 a, g dbSNP:750917268
2557 2557 c, t dbSNP:779465924
2558 2558 c, g dbSNP:549958385
2560 2560 c, t dbSNP:754207409
2561 2561 a, g dbSNP:764545113
2567 2567 c, g dbSNP:1051889
2574 2574 a, c dbSNP:760772767
2575 2575 g, t dbSNP:752977430
2579 2579 c, g, t dbSNP:201933531
2585 2585 a, g dbSNP:774397515
2587 2587 a, g dbSNP:771039657
2594 2594 a, c, t dbSNP:772842482
2598 2598 c, t dbSNP:750221534
2650 2650 a, c dbSNP:752553102
2656 2656 -, a dbSNP:755301025
2662 2662 a, t dbSNP:765040142
2719 2719 a, t dbSNP:567517676
2724 2724 c, t dbSNP:564793153
2751 2751 a, g dbSNP:548886681
2771 2771 c, g dbSNP:767478639
2794 2794 g, t dbSNP:527607188
2858 2858 c, t dbSNP:754978001
2868 2868 c, t dbSNP:559998304
2883 2883 a, g dbSNP:551209662
2897 2897 a, g dbSNP:761836715
2907 2907 a, g dbSNP:532938712
2917 2917 c, t dbSNP:562612539
2976 2976 a, g dbSNP:543939301
2997 2997 c, t dbSNP:6533526
2998 2998 g, t dbSNP:561697335
3014 3014 a, g dbSNP:75911264
3033 3033 c, t dbSNP:144573985
3052 3052 -, attat dbSNP:754182898
3052 3052 a, g dbSNP:554499964
3060 3060 -, tattat dbSNP:764176907
3060 3060 -, tat dbSNP:775662096
3062 3062 -, tat dbSNP:569839843
3063 3063 -, tat dbSNP:561702585
3065 3065 c, t dbSNP:188349821
3100 3100 c, g dbSNP:2739201
3102 3102 c, g dbSNP:2739202
3118 3118 c, t dbSNP:759153090
3133 3133 -, ta dbSNP:200739974
3136 3136 a, g dbSNP:760397096
3140 3140 a, g dbSNP:772929558
3174 3174 -, att dbSNP:767699594
3230 3230 a, g dbSNP:185925533

Target ORF information:

RefSeq Version NM_153426
Organism Homo sapiens (human)
Definition Homo sapiens paired-like homeodomain 2 (PITX2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_153426

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACCA ACTGCCGCAA ACTGGTGTCG GCGTGTGTGC AATTAGGCGT GCAGCCGGCG 
GCCGTTGAAT GTCTCTTCTC CAAAGACTCC GAAATCAAAA AGGTCGAGTT CACGGACTCT
CCTGAGAGCC GAAAAGAGGC AGCCAGCAGC AAGTTCTTCC CGCGGCAGCA TCCTGGCGCC
AATGAGAAAG ATAAAAGCCA GCAGGGGAAG AATGAGGACG TGGGCGCCGA GGACCCGTCT
AAGAAGAAGC GGCAAAGGCG GCAGCGGACT CACTTTACCA GCCAGCAGCT CCAGGAGCTG
GAGGCCACTT TCCAGAGGAA CCGCTACCCG GACATGTCCA CACGCGAAGA AATCGCTGTG
TGGACCAACC TTACGGAAGC CCGAGTCCGG GTTTGGTTCA AGAATCGTCG GGCCAAATGG
AGAAAGAGGG AGCGCAACCA GCAGGCCGAG CTATGCAAGA ATGGCTTCGG GCCGCAGTTC
AATGGGCTCA TGCAGCCCTA CGACGACATG TACCCAGGCT ATTCCTACAA CAACTGGGCC
GCCAAGGGCC TTACATCCGC CTCCCTATCC ACCAAGAGCT TCCCCTTCTT CAACTCTATG
AACGTCAACC CCCTGTCATC ACAGAGCATG TTTTCCCCAC CCAACTCTAT CTCGTCCATG
AGCATGTCGT CCAGCATGGT GCCCTCAGCA GTGACAGGCG TCCCGGGCTC CAGTCTCAAC
AGCCTGAATA ACTTGAACAA CCTGAGTAGC CCGTCGCTGA ATTCCGCGGT GCCGACGCCT
GCCTGTCCTT ACGCGCCGCC GACTCCTCCG TATGTTTATA GGGACACGTG TAACTCGAGC
CTGGCCAGCC TGAGACTGAA AGCAAAGCAG CACTCCAGCT TCGGCTACGC CAGCGTGCAG
AACCCGGCCT CCAACCTGAG TGCTTGCCAG TATGCAGTGG ACCGGCCCGT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17480
Accession Version NM_153427.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 816bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pituitary homeobox 2 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP372081.1, AK127829.1, BX380370.2, U69961.1 and BC106010.1. This sequence is a reference standard in the RefSeqGene project. On Mar 1, 2011 this sequence version replaced gi:24234710. Summary: This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. The encoded protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. This protein plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdominal organs, and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this gene are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1), also known as ARP1a, lacks an in-frame exon in the 5' region, as compared to variant 2. The resulting isoform (a) lacks an internal segment, as compared to isoform b. Variants 1 and 6 encode the same isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U69961.1, AF048720.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGAGACCA ACTGCCGCAA ACTGGTGTCG GCGTGTGTGC AATTAGAGAA AGATAAAAGC 
CAGCAGGGGA AGAATGAGGA CGTGGGCGCC GAGGACCCGT CTAAGAAGAA GCGGCAAAGG
CGGCAGCGGA CTCACTTTAC CAGCCAGCAG CTCCAGGAGC TGGAGGCCAC TTTCCAGAGG
AACCGCTACC CGGACATGTC CACACGCGAA GAAATCGCTG TGTGGACCAA CCTTACGGAA
GCCCGAGTCC GGGTTTGGTT CAAGAATCGT CGGGCCAAAT GGAGAAAGAG GGAGCGCAAC
CAGCAGGCCG AGCTATGCAA GAATGGCTTC GGGCCGCAGT TCAATGGGCT CATGCAGCCC
TACGACGACA TGTACCCAGG CTATTCCTAC AACAACTGGG CCGCCAAGGG CCTTACATCC
GCCTCCCTAT CCACCAAGAG CTTCCCCTTC TTCAACTCTA TGAACGTCAA CCCCCTGTCA
TCACAGAGCA TGTTTTCCCC ACCCAACTCT ATCTCGTCCA TGAGCATGTC GTCCAGCATG
GTGCCCTCAG CAGTGACAGG CGTCCCGGGC TCCAGTCTCA ACAGCCTGAA TAACTTGAAC
AACCTGAGTA GCCCGTCGCT GAATTCCGCG GTGCCGACGC CTGCCTGTCC TTACGCGCCG
CCGACTCCTC CGTATGTTTA TAGGGACACG TGTAACTCGA GCCTGGCCAG CCTGAGACTG
AAAGCAAAGC AGCACTCCAG CTTCGGCTAC GCCAGCGTGC AGAACCCGGC CTCCAACCTG
AGTGCTTGCC AGTATGCAGT GGACCGGCCC GTGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_700476.1
CDS1590..2405
Misc Feature(1)1581..1583(+)
Misc Feature(2)1707..1883(+)
Misc Feature(3)1707..1877(+)
Misc Feature(4)1713..1865(+)
Misc Feature(5)2271..2327(+)
Exon (1)1..205
Gene:PITX2
Gene Synonym:
Exon (2)206..1579
Gene:PITX2
Gene Synonym:
Exon (3)1580..1635
Gene:PITX2
Gene Synonym:
Exon (4)1636..1841
Gene:PITX2
Gene Synonym:
Exon (5)1842..3107
Gene:PITX2
Gene Synonym:
Translation
Position Chain Variation Link
26 26 c, g dbSNP:746939015
28 28 g, t dbSNP:535584251
31 31 g, t dbSNP:549860822
58 58 a, c dbSNP:117231596
80 80 a, c dbSNP:777788477
133 133 c, t dbSNP:560563915
165 165 g, t dbSNP:568054450
169 169 c, g dbSNP:758425186
174 174 a, g dbSNP:752107218
239 239 c, t dbSNP:528361609
241 241 -, c dbSNP:61567593
255 255 a, c dbSNP:145638916
279 279 g, t dbSNP:192250966
286 286 a, g dbSNP:530599491
304 304 -, aa dbSNP:58693505
318 318 a, t dbSNP:774962199
331 331 -, g dbSNP:776236506
331 331 g, t dbSNP:375830124
353 353 c, g dbSNP:747895666
381 381 c, t dbSNP:563409386
439 439 c, g dbSNP:541903355
453 453 a, g dbSNP:141922822
479 479 c, g dbSNP:187210297
498 498 a, g dbSNP:754809224
522 522 c, t dbSNP:62338989
531 531 -, cttcccc dbSNP:548594714
534 534 a, c dbSNP:569838118
549 549 c, g dbSNP:2739191
553 553 c, g dbSNP:2739192
566 566 a, g dbSNP:181439547
583 583 c, t dbSNP:377371567
607 607 c, g dbSNP:2739193
610 610 c, g dbSNP:2739194
623 623 c, t dbSNP:368647502
670 670 c, t dbSNP:779178803
676 676 -, gg dbSNP:531052822
684 684 c, g dbSNP:369582630
758 758 a, c dbSNP:138899981
781 781 a, c dbSNP:569100114
797 797 a, c dbSNP:145044365
856 856 c, t dbSNP:189152290
865 865 c, t dbSNP:568128651
921 921 c, t dbSNP:546942744
926 926 a, c dbSNP:528451197
964 964 c, t dbSNP:570755690
995 995 c, t dbSNP:185557577
1041 1041 a, c dbSNP:13145739
1070 1070 g, t dbSNP:755517957
1105 1105 c, g dbSNP:2739200
1110 1110 g, t dbSNP:563434293
1111 1111 c, t dbSNP:548322604
1121 1121 c, t dbSNP:529787081
1172 1172 -, c dbSNP:111246791
1173 1173 -, c dbSNP:551766296
1183 1183 a, g dbSNP:559470704
1191 1191 a, c dbSNP:35956624
1198 1198 g, t dbSNP:113778706
1228 1228 c, g dbSNP:181197472
1230 1230 a, c dbSNP:564846436
1239 1239 a, c dbSNP:546132428
1278 1278 a, g dbSNP:776489864
1296 1296 c, t dbSNP:149097993
1303 1303 a, g dbSNP:113717851
1306 1306 -, ag dbSNP:774880258
1379 1379 a, g dbSNP:111733107
1385 1385 a, c dbSNP:535573526
1399 1399 a, g dbSNP:371965728
1451 1451 c, t dbSNP:574619224
1468 1468 g, t dbSNP:553173018
1511 1511 a, g dbSNP:535056127
1561 1561 a, g dbSNP:570712814
1570 1570 a, c dbSNP:2739203
1591 1591 g, t dbSNP:75334582
1595 1595 a, g dbSNP:376818069
1602 1602 c, g, t dbSNP:200683912
1613 1613 a, g, t dbSNP:143778014
1616 1616 a, g, t dbSNP:531925274
1617 1617 a, t dbSNP:139906074
1619 1619 c, g dbSNP:746215581
1621 1621 c, t dbSNP:373848340
1622 1622 c, g dbSNP:370048347
1625 1625 c, t dbSNP:749410556
1626 1626 c, g dbSNP:1131942
1627 1627 g, t dbSNP:374524467
1633 1633 c, t dbSNP:778111934
1643 1643 c, t dbSNP:370588233
1651 1651 a, c, t dbSNP:753935193
1652 1652 a, g dbSNP:756527247
1654 1654 a, c, g dbSNP:201628949
1662 1662 a, c dbSNP:781343571
1664 1664 a, t dbSNP:755404910
1671 1671 a, g dbSNP:535881495
1675 1675 a, g dbSNP:766774222
1678 1678 c, g dbSNP:763095137
1679 1679 c, g dbSNP:767533645
1680 1680 a, g dbSNP:765382079
1698 1698 -, aag dbSNP:770651665
1702 1702 a, c, g dbSNP:28936409
1703 1703 a, g dbSNP:149288560
1712 1712 g, t dbSNP:768386257
1728 1728 a, c dbSNP:760524995
1733 1733 c, t dbSNP:775089001
1746 1746 c, g dbSNP:1051887
1750 1750 a, t dbSNP:104893857
1774 1774 a, g dbSNP:104893862
1791 1791 a, c dbSNP:104893858
1795 1795 a, g dbSNP:104893861
1832 1832 c, t dbSNP:771757537
1836 1836 c, g dbSNP:121909249
1838 1838 c, g dbSNP:745540247
1839 1839 c, t dbSNP:121909248
1846 1846 g, t dbSNP:772800095
1851 1851 a, g dbSNP:387906810
1859 1859 c, t dbSNP:769461476
1861 1861 c, g dbSNP:104893859
1873 1873 a, g dbSNP:747454924
1874 1874 a, g dbSNP:138593200
1880 1880 a, g dbSNP:776157834
1885 1885 g, t dbSNP:772313984
1886 1886 c, t dbSNP:770354450
1892 1892 a, g dbSNP:746315869
1898 1898 a, c dbSNP:778975957
1907 1907 c, t dbSNP:757448249
1916 1916 c, t dbSNP:374404013
1918 1918 g, t dbSNP:777760630
1919 1919 c, t dbSNP:756196786
1925 1925 g, t dbSNP:752617007
1928 1928 a, g dbSNP:781170593
1933 1933 a, g dbSNP:754698054
1951 1951 a, g dbSNP:751418961
1955 1955 c, t dbSNP:113012179
1960 1960 c, t dbSNP:766110695
1965 1965 c, t dbSNP:527498517
1978 1978 a, g dbSNP:750107133
1988 1988 a, g dbSNP:104893860
1992 1992 a, g dbSNP:77144743
1997 1997 a, g dbSNP:781375019
2000 2000 c, t dbSNP:776139719
2003 2003 c, t dbSNP:772679916
2006 2006 a, t dbSNP:759978846
2010 2010 a, g dbSNP:193920830
2016 2016 c, g dbSNP:774733901
2018 2018 a, c, g dbSNP:749452484
2023 2023 c, g dbSNP:544781282
2024 2024 c, g dbSNP:577700410
2035 2035 a, c dbSNP:748266578
2040 2040 g, t dbSNP:780968713
2042 2042 c, t dbSNP:754895399
2043 2043 a, g dbSNP:751484586
2044 2044 a, g dbSNP:779833505
2048 2048 g, t dbSNP:35946364
2049 2049 a, g dbSNP:138163892
2054 2054 c, t dbSNP:765053157
2064 2064 c, t dbSNP:761378970
2069 2069 a, t dbSNP:141176394
2081 2081 c, g dbSNP:763539767
2086 2086 c, g dbSNP:760018349
2100 2100 a, g dbSNP:774730294
2101 2101 g, t dbSNP:771311013
2103 2103 c, t dbSNP:763373195
2105 2105 a, g dbSNP:140541021
2108 2108 c, t dbSNP:201319333
2111 2111 a, g dbSNP:748391268
2114 2114 c, t dbSNP:781291656
2115 2115 a, g dbSNP:768622921
2125 2125 a, g dbSNP:746908680
2150 2150 c, t dbSNP:555260580
2151 2151 a, g dbSNP:758267195
2158 2158 a, g dbSNP:146807994
2159 2159 c, t dbSNP:143714974
2168 2168 c, g dbSNP:756926743
2185 2185 g, t dbSNP:536920164
2204 2204 a, g dbSNP:763598663
2209 2209 c, t dbSNP:755635155
2210 2210 c, g dbSNP:752099801
2216 2216 c, g dbSNP:766833864
2217 2217 c, g, t dbSNP:149181425
2225 2225 g, t dbSNP:145355581
2228 2228 a, c, g dbSNP:776732876
2238 2238 a, c, t dbSNP:747126705
2239 2239 c, t dbSNP:775470219
2242 2242 a, t dbSNP:371238455
2243 2243 c, t dbSNP:745827821
2246 2246 a, g dbSNP:778779105
2247 2247 a, c dbSNP:376861814
2248 2248 c, t dbSNP:777541682
2249 2249 a, g dbSNP:148191851
2251 2251 a, c dbSNP:777353401
2263 2263 a, g dbSNP:755618053
2267 2267 a, t dbSNP:752197021
2277 2277 a, g dbSNP:374542009
2279 2279 a, g dbSNP:767036406
2292 2292 c, t dbSNP:139401187
2296 2296 c, t dbSNP:750934780
2297 2297 c, t dbSNP:573990899
2336 2336 c, g dbSNP:1051888
2337 2337 c, t dbSNP:762224214
2339 2339 c, t dbSNP:776910041
2345 2345 c, g dbSNP:764265570
2346 2346 a, g dbSNP:760790139
2354 2354 g, t dbSNP:2739199
2365 2365 a, g dbSNP:775387051
2366 2366 c, t dbSNP:772278492
2371 2371 a, g dbSNP:571758306
2380 2380 a, g dbSNP:774485351
2395 2395 c, g dbSNP:770843364
2399 2399 a, c, t dbSNP:147543001
2400 2400 c, g, t dbSNP:747865049
2408 2408 c, t dbSNP:780613951
2410 2410 a, c dbSNP:754498609
2411 2411 a, g dbSNP:750917268
2419 2419 c, t dbSNP:779465924
2420 2420 c, g dbSNP:549958385
2422 2422 c, t dbSNP:754207409
2423 2423 a, g dbSNP:764545113
2429 2429 c, g dbSNP:1051889
2436 2436 a, c dbSNP:760772767
2437 2437 g, t dbSNP:752977430
2441 2441 c, g, t dbSNP:201933531
2447 2447 a, g dbSNP:774397515
2449 2449 a, g dbSNP:771039657
2456 2456 a, c, t dbSNP:772842482
2460 2460 c, t dbSNP:750221534
2512 2512 a, c dbSNP:752553102
2518 2518 -, a dbSNP:755301025
2524 2524 a, t dbSNP:765040142
2581 2581 a, t dbSNP:567517676
2586 2586 c, t dbSNP:564793153
2613 2613 a, g dbSNP:548886681
2633 2633 c, g dbSNP:767478639
2656 2656 g, t dbSNP:527607188
2720 2720 c, t dbSNP:754978001
2730 2730 c, t dbSNP:559998304
2745 2745 a, g dbSNP:551209662
2759 2759 a, g dbSNP:761836715
2769 2769 a, g dbSNP:532938712
2779 2779 c, t dbSNP:562612539
2838 2838 a, g dbSNP:543939301
2859 2859 c, t dbSNP:6533526
2860 2860 g, t dbSNP:561697335
2876 2876 a, g dbSNP:75911264
2895 2895 c, t dbSNP:144573985
2914 2914 -, attat dbSNP:754182898
2914 2914 a, g dbSNP:554499964
2922 2922 -, tattat dbSNP:764176907
2922 2922 -, tat dbSNP:775662096
2924 2924 -, tat dbSNP:569839843
2925 2925 -, tat dbSNP:561702585
2927 2927 c, t dbSNP:188349821
2962 2962 c, g dbSNP:2739201
2964 2964 c, g dbSNP:2739202
2980 2980 c, t dbSNP:759153090
2995 2995 -, ta dbSNP:200739974
2998 2998 a, g dbSNP:760397096
3002 3002 a, g dbSNP:772929558
3036 3036 -, att dbSNP:767699594
3092 3092 a, g dbSNP:185925533

Target ORF information:

RefSeq Version NM_153427
Organism Homo sapiens (human)
Definition Homo sapiens paired-like homeodomain 2 (PITX2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_153427

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGAGACCA ACTGCCGCAA ACTGGTGTCG GCGTGTGTGC AATTAGAGAA AGATAAAAGC 
CAGCAGGGGA AGAATGAGGA CGTGGGCGCC GAGGACCCGT CTAAGAAGAA GCGGCAAAGG
CGGCAGCGGA CTCACTTTAC CAGCCAGCAG CTCCAGGAGC TGGAGGCCAC TTTCCAGAGG
AACCGCTACC CGGACATGTC CACACGCGAA GAAATCGCTG TGTGGACCAA CCTTACGGAA
GCCCGAGTCC GGGTTTGGTT CAAGAATCGT CGGGCCAAAT GGAGAAAGAG GGAGCGCAAC
CAGCAGGCCG AGCTATGCAA GAATGGCTTC GGGCCGCAGT TCAATGGGCT CATGCAGCCC
TACGACGACA TGTACCCAGG CTATTCCTAC AACAACTGGG CCGCCAAGGG CCTTACATCC
GCCTCCCTAT CCACCAAGAG CTTCCCCTTC TTCAACTCTA TGAACGTCAA CCCCCTGTCA
TCACAGAGCA TGTTTTCCCC ACCCAACTCT ATCTCGTCCA TGAGCATGTC GTCCAGCATG
GTGCCCTCAG CAGTGACAGG CGTCCCGGGC TCCAGTCTCA ACAGCCTGAA TAACTTGAAC
AACCTGAGTA GCCCGTCGCT GAATTCCGCG GTGCCGACGC CTGCCTGTCC TTACGCGCCG
CCGACTCCTC CGTATGTTTA TAGGGACACG TGTAACTCGA GCCTGGCCAG CCTGAGACTG
AAAGCAAAGC AGCACTCCAG CTTCGGCTAC GCCAGCGTGC AGAACCCGGC CTCCAACCTG
AGTGCTTGCC AGTATGCAGT GGACCGGCCC GTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17360
Accession Version NM_000325.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 975bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pituitary homeobox 2 isoform c
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC017068.7 and BC013998.2. On Jul 24, 2007 this sequence version replaced gi:40316913. Summary: This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. The encoded protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. This protein plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdominal organs, and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this gene are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (3), also known as ARP1c, lacks several exons at the 5' end and has an alternate 5' exon, as compared to variant 2. The resulting isoform (c) has a longer and distinct N-terminus, as compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC013998.2, AK313987.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162568 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

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661
721
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841
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961
ATGAACTGCA TGAAAGGCCC GCTTCACTTG GAGCACCGAG CAGCGGGGAC CAAGCTGTCG 
GCCGTCTCCT CATCTTCCTG TCACCATCCC CAGCCGTTAG CCATGGCTTC GGTTCTGGCT
CCCGGTCAGC CCCGGTCGCT GGACTCCTCC AAGCACAGGC TGGAGGTGCA CACCATCTCC
GACACCTCCA GCCCGGAGGC CGCAGAGAAA GATAAAAGCC AGCAGGGGAA GAATGAGGAC
GTGGGCGCCG AGGACCCGTC TAAGAAGAAG CGGCAAAGGC GGCAGCGGAC TCACTTTACC
AGCCAGCAGC TCCAGGAGCT GGAGGCCACT TTCCAGAGGA ACCGCTACCC GGACATGTCC
ACACGCGAAG AAATCGCTGT GTGGACCAAC CTTACGGAAG CCCGAGTCCG GGTTTGGTTC
AAGAATCGTC GGGCCAAATG GAGAAAGAGG GAGCGCAACC AGCAGGCCGA GCTATGCAAG
AATGGCTTCG GGCCGCAGTT CAATGGGCTC ATGCAGCCCT ACGACGACAT GTACCCAGGC
TATTCCTACA ACAACTGGGC CGCCAAGGGC CTTACATCCG CCTCCCTATC CACCAAGAGC
TTCCCCTTCT TCAACTCTAT GAACGTCAAC CCCCTGTCAT CACAGAGCAT GTTTTCCCCA
CCCAACTCTA TCTCGTCCAT GAGCATGTCG TCCAGCATGG TGCCCTCAGC AGTGACAGGC
GTCCCGGGCT CCAGTCTCAA CAGCCTGAAT AACTTGAACA ACCTGAGTAG CCCGTCGCTG
AATTCCGCGG TGCCGACGCC TGCCTGTCCT TACGCGCCGC CGACTCCTCC GTATGTTTAT
AGGGACACGT GTAACTCGAG CCTGGCCAGC CTGAGACTGA AAGCAAAGCA GCACTCCAGC
TTCGGCTACG CCAGCGTGCA GAACCCGGCC TCCAACCTGA GTGCTTGCCA GTATGCAGTG
GACCGGCCCG TGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000316.2
CDS639..1613
Misc Feature(1)429..431(+)
Misc Feature(2)810..812(+)
Misc Feature(3)816..818(+)
Misc Feature(4)822..824(+)
Misc Feature(5)825..827(+)
Misc Feature(6)828..830(+)
Misc Feature(7)915..1091(+)
Misc Feature(8)915..1085(+)
Misc Feature(9)921..1073(+)
Misc Feature(10)1479..1535(+)
Exon (1)1..843
Gene:PITX2
Gene Synonym:
Exon (2)844..1049
Gene:PITX2
Gene Synonym:
Exon (3)1050..2315
Gene:PITX2
Gene Synonym:
Translation
Position Chain Variation Link
5 5 g, t dbSNP:766665953
21 21 c, t dbSNP:560349547
28 28 c, g dbSNP:573041939
45 45 c, g dbSNP:148820148
47 47 c, t dbSNP:539452328
55 55 -, tg dbSNP:768902116
56 56 g, t dbSNP:569118049
60 60 g, t dbSNP:2595111
108 108 a, t dbSNP:550866477
126 126 a, g dbSNP:545323487
240 240 -, cgt dbSNP:778783909
242 242 -, t dbSNP:762116474
243 243 -, gg dbSNP:564546832
243 243 -, g dbSNP:56195298
244 244 g, t dbSNP:78468841
251 251 -, gg dbSNP:10546315
251 251 -, g dbSNP:764419682
252 252 -, g dbSNP:138238047
253 253 -, cg dbSNP:577719189
253 253 -, c dbSNP:563949186
268 268 a, c dbSNP:535357890
298 298 c, t dbSNP:374348901
311 311 a, g dbSNP:185507493
382 382 -, a dbSNP:34729216
383 383 c, t dbSNP:546511871
403 403 c, t dbSNP:528282642
468 468 g, t dbSNP:373631143
502 502 c, g dbSNP:570605499
519 519 -, g dbSNP:370581020
575 575 a, g dbSNP:552252673
588 588 c, g dbSNP:765800469
592 592 a, g dbSNP:530528602
606 606 c, t dbSNP:563110908
624 624 a, c dbSNP:764468500
630 630 c, g dbSNP:760914601
634 634 c, g dbSNP:775582988
635 635 g, t dbSNP:772175693
646 646 a, g dbSNP:745998445
648 648 a, t dbSNP:774395470
654 654 g, t dbSNP:200583809
658 658 c, t dbSNP:749259376
661 661 g, t dbSNP:777670230
666 666 a, t dbSNP:755864040
680 680 a, g dbSNP:747777069
685 685 a, g dbSNP:780767938
686 686 c, g dbSNP:754509231
688 688 c, g dbSNP:751109859
693 693 c, t dbSNP:370268988
699 699 a, g dbSNP:765712420
701 701 c, t dbSNP:61756183
707 707 c, t dbSNP:371757955
709 709 c, t dbSNP:764455104
717 717 c, t dbSNP:760964656
725 725 c, t dbSNP:775774487
734 734 a, g dbSNP:767669969
742 742 c, t dbSNP:369021240
749 749 c, g dbSNP:774520241
759 759 c, t dbSNP:143452464
762 762 c, g dbSNP:749171340
768 768 c, t dbSNP:773293936
771 771 a, c dbSNP:769617540
772 772 c, g dbSNP:747962392
775 775 a, c dbSNP:780866744
792 792 c, g dbSNP:754636613
798 798 c, t dbSNP:137987101
799 799 a, t dbSNP:28936408
802 802 a, t dbSNP:779502717
805 805 c, t dbSNP:757913059
817 817 c, t dbSNP:377094876
818 818 c, t dbSNP:754290007
822 822 a, g dbSNP:764572161
835 835 a, g dbSNP:756465683
841 841 c, g dbSNP:753049637
851 851 c, t dbSNP:370588233
859 859 a, c, t dbSNP:753935193
860 860 a, g dbSNP:756527247
862 862 a, c, g dbSNP:201628949
870 870 a, c dbSNP:781343571
872 872 a, t dbSNP:755404910
879 879 a, g dbSNP:535881495
883 883 a, g dbSNP:766774222
886 886 c, g dbSNP:763095137
887 887 c, g dbSNP:767533645
888 888 a, g dbSNP:765382079
906 906 -, aag dbSNP:770651665
910 910 a, c, g dbSNP:28936409
911 911 a, g dbSNP:149288560
920 920 g, t dbSNP:768386257
936 936 a, c dbSNP:760524995
941 941 c, t dbSNP:775089001
954 954 c, g dbSNP:1051887
958 958 a, t dbSNP:104893857
982 982 a, g dbSNP:104893862
999 999 a, c dbSNP:104893858
1003 1003 a, g dbSNP:104893861
1040 1040 c, t dbSNP:771757537
1044 1044 c, g dbSNP:121909249
1046 1046 c, g dbSNP:745540247
1047 1047 c, t dbSNP:121909248
1054 1054 g, t dbSNP:772800095
1059 1059 a, g dbSNP:387906810
1067 1067 c, t dbSNP:769461476
1069 1069 c, g dbSNP:104893859
1081 1081 a, g dbSNP:747454924
1082 1082 a, g dbSNP:138593200
1088 1088 a, g dbSNP:776157834
1093 1093 g, t dbSNP:772313984
1094 1094 c, t dbSNP:770354450
1100 1100 a, g dbSNP:746315869
1106 1106 a, c dbSNP:778975957
1115 1115 c, t dbSNP:757448249
1124 1124 c, t dbSNP:374404013
1126 1126 g, t dbSNP:777760630
1127 1127 c, t dbSNP:756196786
1133 1133 g, t dbSNP:752617007
1136 1136 a, g dbSNP:781170593
1141 1141 a, g dbSNP:754698054
1159 1159 a, g dbSNP:751418961
1163 1163 c, t dbSNP:113012179
1168 1168 c, t dbSNP:766110695
1173 1173 c, t dbSNP:527498517
1186 1186 a, g dbSNP:750107133
1196 1196 a, g dbSNP:104893860
1200 1200