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PKLR pyruvate kinase, liver and RBC [Homo sapiens (human)]

Gene Symbol PKLR
Entrez Gene ID 5313
Full Name pyruvate kinase, liver and RBC
Synonyms PK1, PKL, PKR, PKRL, RPK
General protein information
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q21

Summary The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

609712

Disorder Html: Pyruvate kinase deficiency, 266200 (3); Adenosine triphosphate,
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu34476 XM_006726212 PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu59730 XM_011546931 PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu34476 XM_011546932 PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu34475 XM_005245266 PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu34476 XM_006711386 PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu59731 XM_011509639 PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu34476 XM_011509640 PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu26995 NM_000298 Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu27268 NM_181871 Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu34476D
Sequence Information ORF Nucleotide Sequence (Length: 1533bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pyruvate kinase PKLR isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003315906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578839333. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCAGACA CCTTCCTGGA ACACCTCTGC CTACTGGACA TTGACTCCGA GCCCGTGGCT 
GCTCGCAGTA CCAGCATCAT TGCCACCATC GGGCCAGCAT CTCGCTCCGT GGAGCGCCTC
AAGGAGATGA TCAAGGCCGG GATGAACATT GCGCGACTCA ACTTCTCCCA CGGCTCCCAC
GAGTACCATG CTGAGTCCAT CGCCAACGTC CGGGAGGCGG TGGAGAGCTT TGCAGGTTCC
CCACTCAGCT ACCGGCCCGT GGCCATCGCC CTGGACACCA AGGGACCGGA GATCCGCACT
GGGATCCTGC AGGGGGGTCC AGAGTCGGAA GTGGAGCTGG TGAAGGGCTC CCAGGTGCTG
GTGACTGTGG ACCCCGCGTT CCGGACGCGG GGGAACGCGA ACACCGTGTG GGTGGACTAC
CCCAATATTG TCCGGGTCGT GCCGGTGGGG GGCCGCATCT ACATTGACGA CGGGCTCATC
TCCCTAGTGG TCCAGAAAAT CGGCCCAGAG GGACTGGTGA CCCAAGTGGA GAACGGCGGC
GTCCTGGGCA GCCGGAAGGG CGTGAACTTG CCAGGGGCCC AGGTGGACTT GCCCGGGCTG
TCCGAGCAGG ACGTCCGAGA CCTGCGCTTC GGGGTGGAGC ATGGGGTGGA CATCGTCTTT
GCCTCCTTTG TGCGGAAAGC CAGCGACGTG GCTGCCGTCA GGGCTGCTCT GGGTCCGGAA
GGACACGGCA TCAAGATCAT CAGCAAAATT GAGAACCACG AAGGCGTGAA GAGGTTTGAT
GAAATCCTGG AGGTGAGCGA CGGCATCATG GTGGCACGGG GGGACCTAGG CATCGAGATC
CCAGCAGAGA AGGTTTTCCT GGCTCAGAAG ATGATGATTG GGCGCTGCAA CTTGGCGGGC
AAGCCTGTTG TCTGTGCCAC ACAGATGCTG GAGAGCATGA TTACCAAGCC CCGGCCAACG
AGGGCAGAGA CAAGCGATGT CGCCAATGCT GTGCTGGATG GGGCTGACTG CATCATGCTG
TCAGGGGAGA CTGCCAAGGG CAACTTCCCT GTGGAAGCGG TGAAGATGCA GCATGCGATT
GCCCGGGAGG CAGAGGCCGC AGTGTACCAC CGGCAGCTGT TTGAGGAGCT ACGTCGGGCA
GCGCCACTAA GCCGTGATCC CACTGAGGTC ACCGCCATTG GTGCTGTGGA GGCTGCCTTC
AAGTGCTGTG CTGCTGCCAT CATTGTGCTG ACCACAACTG GCCGCTCAGC CCAGCTTCTG
TCTCGGTACC GACCTCGGGC AGCAGTCATT GCTGTCACCC GCTCTGCCCA GGCTGCCCGC
CAGGTCCACT TATGCCGAGG AGTCTTCCCC TTGCTTTACC GTGAACCTCC AGAAGCCATC
TGGGCAGATG ATGTAGATCG CCGGGTGCAA TTTGGCATTG AAAGTGGAAA GCTCCGTGGC
TTCCTCCGTG TTGGAGACCT GGTGATTGTG GTGACAGGCT GGCGACCTGG CTCCGGCTAC
ACCAACATCA TGCGGGTGCT AAGCATATCC TGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_006726275.1
CDS126..1658
Misc Feature(1)186..1652(+)
Misc Feature(2)189..1643(+)
Misc Feature(3)192..1466(+)
Misc Feature(4)279..1046(+)
Translation
Position Chain Variation Link
5 5 a, t dbSNP:747479690
9 9 c, t dbSNP:780460339
18 18 a, g dbSNP:375257968
20 20 c, t dbSNP:746119630
26 26 c, g dbSNP:778939180
27 27 c, t dbSNP:757391113
28 28 c, t dbSNP:753922649
30 30 a, t dbSNP:777978713
34 34 c, t dbSNP:762350074
49 49 a, g dbSNP:372152236
50 50 c, t dbSNP:766209695
53 53 g, t dbSNP:758061395
62 62 c, t dbSNP:137996782
63 63 c, t dbSNP:368867132
67 67 c, g dbSNP:761502347
69 69 c, g dbSNP:776156268
73 73 c, g dbSNP:373541741
76 76 a, g dbSNP:763586752
86 86 a, g dbSNP:761224118
92 92 a, g dbSNP:775921590
95 95 g, t dbSNP:772493914
96 96 g, t dbSNP:746180822
114 114 c, t dbSNP:8177962
126 126 a, g dbSNP:773589949
140 140 c, t dbSNP:770187539
145 145 a, t dbSNP:754696137
149 149 c, t dbSNP:762094018
150 150 c, t dbSNP:200791251
151 151 a, t dbSNP:140571200
156 156 c, t dbSNP:768765063
167 167 c, t dbSNP:745802541
171 171 a, t dbSNP:766051969
173 173 c, t dbSNP:185441545
174 174 a, g dbSNP:139707114
177 177 c, g dbSNP:151005140
179 179 c, t dbSNP:777525158
180 180 a, g dbSNP:368492204
181 181 c, t dbSNP:754785851
189 189 c, g dbSNP:752391774
190 190 a, g dbSNP:375471342
192 192 a, t dbSNP:754454017
205 205 c, t dbSNP:752037743
215 215 c, t dbSNP:147391950
216 216 a, g, t dbSNP:750857114
218 218 a, g dbSNP:759358280
221 221 a, g dbSNP:762937077
228 228 c, t dbSNP:148636159
229 229 a, g dbSNP:534895384
231 231 a, t dbSNP:762661462
233 233 c, g dbSNP:776268137
234 234 g, t dbSNP:143984223
240 240 a, c, t dbSNP:779765565
241 241 a, g dbSNP:371471086
248 248 a, g dbSNP:757921751
267 267 a, g dbSNP:746377052
274 274 c, t dbSNP:779517988
280 280 a, g, t dbSNP:754441130
283 283 g, t dbSNP:754282523
287 287 c, t dbSNP:764337069
301 301 c, g dbSNP:756428691
306 306 c, g dbSNP:752905261
307 307 a, c dbSNP:767598564
318 318 a, g dbSNP:773981429
322 322 a, c dbSNP:118204089
331 331 a, t dbSNP:770337533
332 332 a, c dbSNP:749817706
334 334 a, t dbSNP:574051756
336 336 a, c dbSNP:770175821
348 348 a, g dbSNP:748560715
349 349 a, t dbSNP:757997111
353 353 a, c dbSNP:781504670
365 365 c, t dbSNP:755258394
379 379 a, g dbSNP:757378318
388 388 c, g dbSNP:751716279
393 393 a, g dbSNP:780192373
396 396 c, t dbSNP:758341301
402 402 a, c dbSNP:753840260
407 407 a, g dbSNP:763884887
413 413 a, g dbSNP:146187567
420 420 c, t dbSNP:118204083
431 431 c, t dbSNP:771412921
432 432 c, t dbSNP:767419098
433 433 c, t dbSNP:759323460
438 438 c, g, t dbSNP:199824528
439 439 a, g dbSNP:762330662
440 440 a, g dbSNP:201674983
441 441 a, g dbSNP:779196057
447 447 c, g dbSNP:757359024
456 456 a, g dbSNP:370513342
457 457 c, t dbSNP:781236222
462 462 c, t dbSNP:138482544
465 465 a, g dbSNP:751361992
493 493 c, t dbSNP:370932346
495 495 g, t dbSNP:758042780
498 498 c, g dbSNP:577247981
512 512 a, g dbSNP:764731242
514 514 g, t dbSNP:552916457
518 518 g, t dbSNP:754361540
519 519 a, g dbSNP:781165565
521 521 c, g dbSNP:764631858
523 523 c, t dbSNP:761210207
527 527 a, c dbSNP:775787566
535 535 a, g dbSNP:772473652
548 548 -, c dbSNP:749541053
550 550 a, g dbSNP:759694655
551 551 c, t dbSNP:749833572
553 553 g, t dbSNP:780259014
559 559 a, g dbSNP:200480300
560 560 c, g dbSNP:749332866
564 564 c, g dbSNP:777980472
567 567 c, t dbSNP:768541145
568 568 c, t dbSNP:777667459
569 569 g, t dbSNP:779725913
571 571 c, t dbSNP:200979497
572 572 g, t dbSNP:750102822
578 578 a, c dbSNP:778484808
582 582 a, c dbSNP:756878847
588 588 a, t dbSNP:753384013
589 589 c, t dbSNP:200572803
590 590 c, t dbSNP:756559416
596 596 c, t dbSNP:761145518
599 599 a, g dbSNP:555325288
604 604 a, t dbSNP:768657605
607 607 c, t dbSNP:767781894
608 608 c, t dbSNP:759941126
613 613 a, t dbSNP:774736844
615 615 a, g dbSNP:771329959
619 619 a, g dbSNP:763237413
624 624 a, g dbSNP:764375399
627 627 g, t dbSNP:750606318
632 632 a, g dbSNP:765262843
640 640 c, t dbSNP:769475930
654 654 g, t dbSNP:201953584
655 655 a, t dbSNP:776546824
659 659 c, t dbSNP:772083920
660 660 a, g dbSNP:745797890
663 663 a, g dbSNP:759517847
664 664 a, g dbSNP:774192277
666 666 a, g dbSNP:555712170
678 678 c, t dbSNP:748947397
679 679 a, g dbSNP:368830326
680 680 a, g dbSNP:755602921
684 684 a, g dbSNP:267598065
689 689 c, g dbSNP:747744073
692 692 c, t dbSNP:780539049
698 698 a, g dbSNP:755610815
702 702 a, g dbSNP:752053420
703 703 c, t dbSNP:766853199
708 708 a, g dbSNP:750161685
711 711 a, c, g dbSNP:140575553
713 713 a, c dbSNP:765541284
716 716 g, t dbSNP:761905811
719 719 c, t dbSNP:148319141
723 723 c, t dbSNP:199959447
727 727 c, g dbSNP:769783555
732 732 a, c dbSNP:764143249
737 737 c, t dbSNP:576755609
738 738 g, t dbSNP:200695047
739 739 c, t dbSNP:770747923
741 741 a, c, g dbSNP:772860949
747 747 c, g dbSNP:147659527
750 750 c, t dbSNP:747753310
751 751 a, g dbSNP:141393570
756 756 a, c, g dbSNP:747549978
759 759 a, g dbSNP:780659178
759 759 -, g dbSNP:754939638
762 762 a, g dbSNP:147689373
767 767 c, t dbSNP:750836008
769 769 a, g, t dbSNP:758560647
770 770 c, g dbSNP:757554998
779 779 -, c dbSNP:751449001
791 791 a, c dbSNP:150532071
799 799 a, g dbSNP:749666187
802 802 a, g dbSNP:780875123
807 807 a, t dbSNP:749573131
813 813 g, t dbSNP:760709075
816 816 a, g dbSNP:751524706
817 817 c, t dbSNP:766353400
822 822 a, c, g dbSNP:148435198
829 829 a, c dbSNP:769464933
836 836 a, g dbSNP:761529212
839 839 c, t dbSNP:776382179
840 840 c, g dbSNP:201979697
841 841 c, t dbSNP:746566827
842 842 c, g dbSNP:200840994
851 851 a, c, t dbSNP:141571402
852 852 a, c, g dbSNP:373990660
853 853 g, t dbSNP:754124936
870 870 a, c dbSNP:777926506
872 872 a, g dbSNP:756311123
884 884 a, c, t dbSNP:745663677
886 886 a, g dbSNP:767580478
889 889 a, g dbSNP:763006858
891 891 c, g dbSNP:549295725
899 899 g, t dbSNP:763964110
904 904 a, g dbSNP:760413736
905 905 c, t dbSNP:8177974
912 912 c, g dbSNP:767088632
913 913 a, t dbSNP:758924903
924 924 a, g dbSNP:773893686
926 926 a, c, t dbSNP:138476691
927 927 a, g dbSNP:773626254
935 935 a, g dbSNP:756339496
942 942 c, g, t dbSNP:748376799
944 944 c, g dbSNP:781220573
948 948 a, g, t dbSNP:747097960
953 953 a, g dbSNP:780173822
956 956 c, g dbSNP:757318290
959 959 c, t dbSNP:376694187
960 960 a, g dbSNP:781715321
975 975 -, aag dbSNP:762301937
977 977 a, g dbSNP:763982504
982 982 a, t dbSNP:755934816
1000 1000 c, t dbSNP:752423472
1003 1003 c, t dbSNP:779152555
1008 1008 c, t dbSNP:138871700
1012 1012 c, g dbSNP:773803500
1014 1014 a, g dbSNP:765903674
1021 1021 a, c dbSNP:763466930
1022 1022 a, g dbSNP:773644908
1029 1029 c, g, t dbSNP:533316562
1032 1032 g, t dbSNP:777020292
1037 1037 -, ctgt dbSNP:749942178
1055 1055 a, g dbSNP:754739652
1061 1061 c, t dbSNP:751204824
1070 1070 c, t dbSNP:779728345
1075 1075 c, t dbSNP:758011557
1077 1077 a, c, t dbSNP:140964046
1079 1079 a, g dbSNP:764709204
1080 1080 c, g, t dbSNP:767333188
1082 1082 a, t dbSNP:764396178
1084 1084 c, t dbSNP:74315362
1085 1085 a, g dbSNP:761048881
1091 1091 a, c dbSNP:775697482
1092 1092 a, g dbSNP:147089210
1099 1099 a, g dbSNP:548802413
1100 1100 c, t dbSNP:772465237
1101 1101 a, g dbSNP:147034239
1102 1102 a, g dbSNP:759834904
1103 1103 c, t dbSNP:141596564
1105 1105 c, t dbSNP:771080781
1106 1106 c, t dbSNP:748044200
1111 1111 a, g dbSNP:776594413
1119 1119 c, t dbSNP:761407486
1135 1135 a, g dbSNP:766913459
1141 1141 c, t dbSNP:768361068
1142 1142 a, g dbSNP:368784290
1149 1149 a, g dbSNP:375456388
1150 1150 a, g dbSNP:779817999
1153 1153 a, t dbSNP:780985369
1165 1165 a, c, g dbSNP:745461639
1168 1168 a, c dbSNP:774006353
1169 1169 c, t dbSNP:756750467
1172 1172 c, t dbSNP:754321902
1179 1179 a, g dbSNP:764713394
1183 1183 c, t dbSNP:756549612
1184 1184 a, g, t dbSNP:767981692
1185 1185 a, g dbSNP:759855502
1194 1194 a, c dbSNP:118204084
1201 1201 a, c dbSNP:763528176
1202 1202 a, g dbSNP:774652817
1203 1203 -, a dbSNP:751363828
1206 1206 a, g dbSNP:756703156
1207 1207 a, c dbSNP:753198136
1210 1210 a, g dbSNP:768002493
1223 1223 c, t dbSNP:755357782
1224 1224 a, g dbSNP:762591322
1236 1236 c, t dbSNP:142238268
1239 1239 c, t dbSNP:778805401
1240 1240 a, g dbSNP:758965704
1251 1251 a, g, t dbSNP:771145576
1257 1257 c, t dbSNP:773396824
1258 1258 a, g dbSNP:370554398
1261 1261 a, g dbSNP:760606711
1267 1267 c, t dbSNP:531294009
1268 1268 a, g dbSNP:771876200
1272 1272 a, c, t dbSNP:774102445
1278 1278 c, t dbSNP:189360283
1279 1279 a, g dbSNP:149932430
1286 1286 c, t dbSNP:777357253
1291 1291 a, c dbSNP:769123407
1296 1296 a, g dbSNP:748700869
1298 1298 c, t dbSNP:146708702
1299 1299 a, g dbSNP:756864028
1302 1302 a, g dbSNP:377022809
1306 1306 a, g dbSNP:755522396
1311 1311 a, g dbSNP:752034960
1312 1312 c, t dbSNP:149946271
1316 1316 a, g dbSNP:758655787
1336 1336 c, g dbSNP:750540943
1341 1341 g, t dbSNP:765388322
1346 1346 a, c dbSNP:761831271
1353 1353 c, t dbSNP:753890733
1355 1355 c, g dbSNP:572233729
1362 1362 a, g dbSNP:759466273
1367 1367 a, c dbSNP:774262110
1368 1368 c, t dbSNP:201406712
1369 1369 a, g dbSNP:118204085
1375 1375 c, t dbSNP:773040738
1379 1379 a, g dbSNP:765024950
1382 1382 g, t dbSNP:761542089
1389 1389 c, t dbSNP:116100695
1390 1390 a, g dbSNP:768210809
1396 1396 a, g dbSNP:369183199
1401 1401 c, t dbSNP:200133000
1402 1402 a, g, t dbSNP:746257586
1409 1409 a, g, t dbSNP:757534925
1411 1411 a, t dbSNP:749456521
1417 1417 c, t dbSNP:141560532
1424 1424 c, t dbSNP:756147719
1425 1425 a, c, t dbSNP:551883218
1426 1426 a, g dbSNP:758327704
1441 1441 c, t dbSNP:750255401
1444 1444 g, t dbSNP:185753709
1445 1445 a, c dbSNP:761558916
1449 1449 a, g dbSNP:8177988
1451 1451 c, t dbSNP:376065116
1452 1452 a, c dbSNP:760152160
1454 1454 c, t dbSNP:201011399
1458 1458 c, t dbSNP:760208078
1462 1462 a, g dbSNP:113403872
1472 1472 c, t dbSNP:371489505
1480 1480 c, t dbSNP:779681839
1485 1485 c, t dbSNP:139002629
1486 1486 a, g dbSNP:771218326
1487 1487 a, t dbSNP:749544468
1490 1490 a, c dbSNP:761819694
1491 1491 a, c, t dbSNP:562318137
1494 1494 c, t dbSNP:756341032
1512 1512 a, g dbSNP:748219831
1527 1527 c, t dbSNP:201255024
1528 1528 a, g dbSNP:758278200
1529 1529 c, g dbSNP:750305814
1532 1532 g, t dbSNP:192697407
1537 1537 c, t dbSNP:754526647
1541 1541 c, t dbSNP:757065547
1547 1547 a, t dbSNP:201217064
1551 1551 g, t dbSNP:763878359
1561 1561 a, g dbSNP:752328700
1562 1562 c, t dbSNP:766970429
1564 1564 g, t dbSNP:759039445
1572 1572 c, t dbSNP:751050425
1573 1573 a, g dbSNP:375525211
1578 1578 a, g dbSNP:190966689
1587 1587 a, g dbSNP:370316462
1588 1588 c, t dbSNP:773628430
1596 1596 g, t dbSNP:770125793
1606 1606 a, g dbSNP:370938255
1608 1608 c, t dbSNP:532230312
1609 1609 -, gacctggc dbSNP:771142025
1619 1619 a, c, t dbSNP:140859641
1632 1632 a, t dbSNP:780275059
1634 1634 c, t dbSNP:771049942
1636 1636 c, t dbSNP:749179500
1637 1637 a, g dbSNP:777435169
1638 1638 a, c, t dbSNP:1052176
1639 1639 a, g dbSNP:61755431
1643 1643 c, g dbSNP:552203379
1646 1646 a, g dbSNP:761092859
1661 1661 a, c, t dbSNP:368150636
1662 1662 a, g dbSNP:765864419
1670 1670 a, c dbSNP:758922618
1671 1671 c, g, t dbSNP:1052177
1672 1672 c, g, t dbSNP:8177994
1673 1673 c, g, t dbSNP:374751259
1678 1678 a, g dbSNP:775724792
1680 1680 a, c dbSNP:772210964
1692 1692 c, t dbSNP:749269783
1696 1696 c, t dbSNP:773153185
1715 1715 a, g dbSNP:569710209
1720 1720 c, t dbSNP:542249964
1730 1730 a, c dbSNP:530076782
1736 1736 c, t dbSNP:563189341
1739 1739 c, t dbSNP:186058494
1743 1743 a, g dbSNP:768976043
1794 1794 a, g dbSNP:762039790
1821 1821 c, t dbSNP:774442850
1823 1823 a, g dbSNP:772906215
1850 1850 a, t dbSNP:577392110
1858 1858 a, t dbSNP:768777565
1867 1867 -, g dbSNP:35957800
1914 1914 a, g dbSNP:749428064
1925 1925 c, t dbSNP:932972
1939 1939 g, t dbSNP:770894965

Target ORF information:

RefSeq Version XM_006726212
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_006726212

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCAGACA CCTTCCTGGA ACACCTCTGC CTACTGGACA TTGACTCCGA GCCCGTGGCT 
GCTCGCAGTA CCAGCATCAT TGCCACCATC GGGCCAGCAT CTCGCTCCGT GGAGCGCCTC
AAGGAGATGA TCAAGGCCGG GATGAACATT GCGCGACTCA ACTTCTCCCA CGGCTCCCAC
GAGTACCATG CTGAGTCCAT CGCCAACGTC CGGGAGGCGG TGGAGAGCTT TGCAGGTTCC
CCACTCAGCT ACCGGCCCGT GGCCATCGCC CTGGACACCA AGGGACCGGA GATCCGCACT
GGGATCCTGC AGGGGGGTCC AGAGTCGGAA GTGGAGCTGG TGAAGGGCTC CCAGGTGCTG
GTGACTGTGG ACCCCGCGTT CCGGACGCGG GGGAACGCGA ACACCGTGTG GGTGGACTAC
CCCAATATTG TCCGGGTCGT GCCGGTGGGG GGCCGCATCT ACATTGACGA CGGGCTCATC
TCCCTAGTGG TCCAGAAAAT CGGCCCAGAG GGACTGGTGA CCCAAGTGGA GAACGGCGGC
GTCCTGGGCA GCCGGAAGGG CGTGAACTTG CCAGGGGCCC AGGTGGACTT GCCCGGGCTG
TCCGAGCAGG ACGTCCGAGA CCTGCGCTTC GGGGTGGAGC ATGGGGTGGA CATCGTCTTT
GCCTCCTTTG TGCGGAAAGC CAGCGACGTG GCTGCCGTCA GGGCTGCTCT GGGTCCGGAA
GGACACGGCA TCAAGATCAT CAGCAAAATT GAGAACCACG AAGGCGTGAA GAGGTTTGAT
GAAATCCTGG AGGTGAGCGA CGGCATCATG GTGGCACGGG GGGACCTAGG CATCGAGATC
CCAGCAGAGA AGGTTTTCCT GGCTCAGAAG ATGATGATTG GGCGCTGCAA CTTGGCGGGC
AAGCCTGTTG TCTGTGCCAC ACAGATGCTG GAGAGCATGA TTACCAAGCC CCGGCCAACG
AGGGCAGAGA CAAGCGATGT CGCCAATGCT GTGCTGGATG GGGCTGACTG CATCATGCTG
TCAGGGGAGA CTGCCAAGGG CAACTTCCCT GTGGAAGCGG TGAAGATGCA GCATGCGATT
GCCCGGGAGG CAGAGGCCGC AGTGTACCAC CGGCAGCTGT TTGAGGAGCT ACGTCGGGCA
GCGCCACTAA GCCGTGATCC CACTGAGGTC ACCGCCATTG GTGCTGTGGA GGCTGCCTTC
AAGTGCTGTG CTGCTGCCAT CATTGTGCTG ACCACAACTG GCCGCTCAGC CCAGCTTCTG
TCTCGGTACC GACCTCGGGC AGCAGTCATT GCTGTCACCC GCTCTGCCCA GGCTGCCCGC
CAGGTCCACT TATGCCGAGG AGTCTTCCCC TTGCTTTACC GTGAACCTCC AGAAGCCATC
TGGGCAGATG ATGTAGATCG CCGGGTGCAA TTTGGCATTG AAAGTGGAAA GCTCCGTGGC
TTCCTCCGTG TTGGAGACCT GGTGATTGTG GTGACAGGCT GGCGACCTGG CTCCGGCTAC
ACCAACATCA TGCGGGTGCT AAGCATATCC TGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu59730D
Sequence Information ORF Nucleotide Sequence (Length: 1185bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pyruvate kinase PKLR isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003315906.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGTCCCGC AGCCCCAGGC CCACACTGAA AGCATGTCGA TCCAGGAGAA CATATCATCC 
CTGCAGCTTC GGTCATGGGT CTCTAAGTCC CAAAGAGACT TAGCAAAGTC CATCCTGATT
GGGGCTCCAG GAGGGCCAGC GGGGTATCTG CGGCGGGCCA GTGTGGCCCA ACTGACCCAG
GAGCTGGGCA CTGCCTTCTT CCAGCAGCAG CAGCTGCCAG CTGCTATGGC AGACACCTTC
CTGGAACACC TCTGCCTACT GGACATTGAC TCCGAGCCCG TGGCTGCTCG CAGTACCAGC
ATCATTGCCA CCATCGGGCC AGCATCTCGC TCCGTGGAGC GCCTCAAGGA GATGATCAAG
GCCGGGATGA ACATTGCGCG ACTCAACTTC TCCCACGGCT CCCACGAGTA CCATGCTGAG
TCCATCGCCA ACGTCCGGGA GGCGGTGGAG AGCTTTGCAG GTTCCCCACT CAGCTACCGG
CCCGTGGCCA TCGCCCTGGA CACCAAGGGA CCGGAGATCC GCACTGGGAT CCTGCAGGGG
GGTCCAGAGT CGGAAGTGGA GCTGGTGAAG GGCTCCCAGG TGCTGGTGAC TGTGGACCCC
GCGTTCCGGA CGCGGGGGAA CGCGAACACC GTGTGGGTGG ACTACCCCAA TATTGTCCGG
GTCGTGCCGG TGGGGGGCCG CATCTACATT GACGACGGGC TCATCTCCCT AGTGGTCCAG
AAAATCGGCC CAGAGGGACT GGTGACCCAA GTGGAGAACG GCGGCGTCCT GGGCAGCCGG
AAGGGCGTGA ACTTGCCAGG GGCCCAGGTG GACTTGCCCG GGCTGTCCGA GCAGGACGTC
CGAGACCTGC GCTTCGGGGT GGAGCATGGG GTGGACATCG TCTTTGCCTC CTTTGTGCGG
AAAGCCAGCG ACGTGGCTGC CGTCAGGGCT GCTCTGGGTC CGGAAGGACA CGGCATCAAG
ATCATCAGCA AAATTGAGAA CCACGAAGGC GTGAAGAGGT TTGATGAAAT CCTGGAGGTG
AGCGACGGCA TCATGGTGGC ACGGGGGGAC CTAGGCATCG AGATCCCAGC AGAGAAGGTT
TTCCTGGCTC AGAAGATGAT GATTGGGCGC TGCAACTTGG CGGGCAAGCC TGTTGTCTGT
GCCACACAGG TCTGGAGTGA GGCCTTGAGA TGCTGGAGAG CATGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011545233.1
CDS12..1196
Misc Feature(1)297..>1160(+)
Misc Feature(2)303..1142(+)
Misc Feature(3)390..1157(+)
Translation
Position Chain Variation Link
11 11 c, t dbSNP:748791106
14 14 a, g dbSNP:778164541
15 15 g, t dbSNP:756692982
19 19 c, t dbSNP:200181342
20 20 a, g dbSNP:374904337
22 22 a, c dbSNP:368741800
24 24 c, t dbSNP:751786807
26 26 c, t dbSNP:766550230
27 27 c, g dbSNP:758498340
32 32 c, t dbSNP:750568645
36 36 a, g dbSNP:765203859
38 38 c, t dbSNP:760728696
43 43 a, c, g, t dbSNP:201169481
49 49 a, c dbSNP:774176341
50 50 a, g dbSNP:139697646
56 56 g, t dbSNP:758112712
64 64 c, t dbSNP:762490269
81 81 c, t dbSNP:772818969
82 82 a, g dbSNP:769174570
87 87 c, t dbSNP:748703432
89 89 a, g dbSNP:750812851
96 96 a, g dbSNP:781610938
100 100 c, g dbSNP:769038526
103 103 a, g dbSNP:200357340
114 114 a, g dbSNP:754783713
121 121 c, g dbSNP:747323509
127 127 c, t dbSNP:780370877
130 130 c, t dbSNP:142735355
133 133 a, g dbSNP:771523060
136 136 c, t dbSNP:150077703
151 151 c, g, t dbSNP:770019694
152 152 a, g dbSNP:148376707
154 154 a, g, t dbSNP:118204087
155 155 -, g dbSNP:762615239
155 155 a, g, t dbSNP:141341788
159 159 c, t dbSNP:758304847
163 163 g, t dbSNP:745811076
165 165 c, t dbSNP:375189218
166 166 a, g, t dbSNP:374805791
173 173 c, t dbSNP:777368485
175 175 a, t dbSNP:753415917
182 182 a, g dbSNP:370696464
192 192 a, g dbSNP:752199380
194 194 a, g dbSNP:767023470
198 198 g, t dbSNP:760029571
199 199 a, g dbSNP:752093404
201 201 a, t dbSNP:760443294
204 204 a, g dbSNP:753089348
211 211 a, t dbSNP:746308116
213 213 c, t dbSNP:772567442
215 215 a, g dbSNP:200581879
225 225 c, t dbSNP:8177962
237 237 a, g dbSNP:773589949
251 251 c, t dbSNP:770187539
256 256 a, t dbSNP:754696137
260 260 c, t dbSNP:762094018
261 261 c, t dbSNP:200791251
262 262 a, t dbSNP:140571200
267 267 c, t dbSNP:768765063
278 278 c, t dbSNP:745802541
282 282 a, t dbSNP:766051969
284 284 c, t dbSNP:185441545
285 285 a, g dbSNP:139707114
288 288 c, g dbSNP:151005140
290 290 c, t dbSNP:777525158
291 291 a, g dbSNP:368492204
292 292 c, t dbSNP:754785851
300 300 c, g dbSNP:752391774
301 301 a, g dbSNP:375471342
303 303 a, t dbSNP:754454017
316 316 c, t dbSNP:752037743
326 326 c, t dbSNP:147391950
327 327 a, g, t dbSNP:750857114
329 329 a, g dbSNP:759358280
332 332 a, g dbSNP:762937077
339 339 c, t dbSNP:148636159
340 340 a, g dbSNP:534895384
342 342 a, t dbSNP:762661462
344 344 c, g dbSNP:776268137
345 345 g, t dbSNP:143984223
351 351 a, c, t dbSNP:779765565
352 352 a, g dbSNP:371471086
359 359 a, g dbSNP:757921751
378 378 a, g dbSNP:746377052
385 385 c, t dbSNP:779517988
391 391 a, g, t dbSNP:754441130
394 394 g, t dbSNP:754282523
398 398 c, t dbSNP:764337069
412 412 c, g dbSNP:756428691
417 417 c, g dbSNP:752905261
418 418 a, c dbSNP:767598564
429 429 a, g dbSNP:773981429
433 433 a, c dbSNP:118204089
442 442 a, t dbSNP:770337533
443 443 a, c dbSNP:749817706
445 445 a, t dbSNP:574051756
447 447 a, c dbSNP:770175821
459 459 a, g dbSNP:748560715
460 460 a, t dbSNP:757997111
464 464 a, c dbSNP:781504670
476 476 c, t dbSNP:755258394
490 490 a, g dbSNP:757378318
499 499 c, g dbSNP:751716279
504 504 a, g dbSNP:780192373
507 507 c, t dbSNP:758341301
513 513 a, c dbSNP:753840260
518 518 a, g dbSNP:763884887
524 524 a, g dbSNP:146187567
531 531 c, t dbSNP:118204083
542 542 c, t dbSNP:771412921
543 543 c, t dbSNP:767419098
544 544 c, t dbSNP:759323460
549 549 c, g, t dbSNP:199824528
550 550 a, g dbSNP:762330662
551 551 a, g dbSNP:201674983
552 552 a, g dbSNP:779196057
558 558 c, g dbSNP:757359024
567 567 a, g dbSNP:370513342
568 568 c, t dbSNP:781236222
573 573 c, t dbSNP:138482544
576 576 a, g dbSNP:751361992
604 604 c, t dbSNP:370932346
606 606 g, t dbSNP:758042780
609 609 c, g dbSNP:577247981
623 623 a, g dbSNP:764731242
625 625 g, t dbSNP:552916457
629 629 g, t dbSNP:754361540
630 630 a, g dbSNP:781165565
632 632 c, g dbSNP:764631858
634 634 c, t dbSNP:761210207
638 638 a, c dbSNP:775787566
646 646 a, g dbSNP:772473652
659 659 -, c dbSNP:749541053
661 661 a, g dbSNP:759694655
662 662 c, t dbSNP:749833572
664 664 g, t dbSNP:780259014
670 670 a, g dbSNP:200480300
671 671 c, g dbSNP:749332866
675 675 c, g dbSNP:777980472
678 678 c, t dbSNP:768541145
679 679 c, t dbSNP:777667459
680 680 g, t dbSNP:779725913
682 682 c, t dbSNP:200979497
683 683 g, t dbSNP:750102822
689 689 a, c dbSNP:778484808
693 693 a, c dbSNP:756878847
699 699 a, t dbSNP:753384013
700 700 c, t dbSNP:200572803
701 701 c, t dbSNP:756559416
707 707 c, t dbSNP:761145518
710 710 a, g dbSNP:555325288
715 715 a, t dbSNP:768657605
718 718 c, t dbSNP:767781894
719 719 c, t dbSNP:759941126
724 724 a, t dbSNP:774736844
726 726 a, g dbSNP:771329959
730 730 a, g dbSNP:763237413
735 735 a, g dbSNP:764375399
738 738 g, t dbSNP:750606318
743 743 a, g dbSNP:765262843
751 751 c, t dbSNP:769475930
765 765 g, t dbSNP:201953584
766 766 a, t dbSNP:776546824
770 770 c, t dbSNP:772083920
771 771 a, g dbSNP:745797890
774 774 a, g dbSNP:759517847
775 775 a, g dbSNP:774192277
777 777 a, g dbSNP:555712170
789 789 c, t dbSNP:748947397
790 790 a, g dbSNP:368830326
791 791 a, g dbSNP:755602921
795 795 a, g dbSNP:267598065
800 800 c, g dbSNP:747744073
803 803 c, t dbSNP:780539049
809 809 a, g dbSNP:755610815
813 813 a, g dbSNP:752053420
814 814 c, t dbSNP:766853199
819 819 a, g dbSNP:750161685
822 822 a, c, g dbSNP:140575553
824 824 a, c dbSNP:765541284
827 827 g, t dbSNP:761905811
830 830 c, t dbSNP:148319141
834 834 c, t dbSNP:199959447
838 838 c, g dbSNP:769783555
843 843 a, c dbSNP:764143249
848 848 c, t dbSNP:576755609
849 849 g, t dbSNP:200695047
850 850 c, t dbSNP:770747923
852 852 a, c, g dbSNP:772860949
858 858 c, g dbSNP:147659527
861 861 c, t dbSNP:747753310
862 862 a, g dbSNP:141393570
867 867 a, c, g dbSNP:747549978
870 870 a, g dbSNP:780659178
870 870 -, g dbSNP:754939638
873 873 a, g dbSNP:147689373
878 878 c, t dbSNP:750836008
880 880 a, g, t dbSNP:758560647
881 881 c, g dbSNP:757554998
890 890 -, c dbSNP:751449001
902 902 a, c dbSNP:150532071
910 910 a, g dbSNP:749666187
913 913 a, g dbSNP:780875123
918 918 a, t dbSNP:749573131
924 924 g, t dbSNP:760709075
927 927 a, g dbSNP:751524706
928 928 c, t dbSNP:766353400
933 933 a, c, g dbSNP:148435198
940 940 a, c dbSNP:769464933
947 947 a, g dbSNP:761529212
950 950 c, t dbSNP:776382179
951 951 c, g dbSNP:201979697
952 952 c, t dbSNP:746566827
953 953 c, g dbSNP:200840994
962 962 a, c, t dbSNP:141571402
963 963 a, c, g dbSNP:373990660
964 964 g, t dbSNP:754124936
981 981 a, c dbSNP:777926506
983 983 a, g dbSNP:756311123
995 995 a, c, t dbSNP:745663677
997 997 a, g dbSNP:767580478
1000 1000 a, g dbSNP:763006858
1002 1002 c, g dbSNP:549295725
1010 1010 g, t dbSNP:763964110
1015 1015 a, g dbSNP:760413736
1016 1016 c, t dbSNP:8177974
1023 1023 c, g dbSNP:767088632
1024 1024 a, t dbSNP:758924903
1035 1035 a, g dbSNP:773893686
1037 1037 a, c, t dbSNP:138476691
1038 1038 a, g dbSNP:773626254
1046 1046 a, g dbSNP:756339496
1053 1053 c, g, t dbSNP:748376799
1055 1055 c, g dbSNP:781220573
1059 1059 a, g, t dbSNP:747097960
1064 1064 a, g dbSNP:780173822
1067 1067 c, g dbSNP:757318290
1070 1070 c, t dbSNP:376694187
1071 1071 a, g dbSNP:781715321
1086 1086 -, aag dbSNP:762301937
1088 1088 a, g dbSNP:763982504
1093 1093 a, t dbSNP:755934816
1111 1111 c, t dbSNP:752423472
1114 1114 c, t dbSNP:779152555
1119 1119 c, t dbSNP:138871700
1123 1123 c, g dbSNP:773803500
1125 1125 a, g dbSNP:765903674
1132 1132 a, c dbSNP:763466930
1133 1133 a, g dbSNP:773644908
1140 1140 c, g, t dbSNP:533316562
1143 1143 g, t dbSNP:777020292
1148 1148 -, ctgt dbSNP:749942178
1162 1162 g, t dbSNP:768823171
1167 1167 a, t dbSNP:201407348
1170 1170 g, t dbSNP:780010017
1171 1171 a, c dbSNP:772210846
1176 1176 g, t dbSNP:749267549
1186 1186 a, g dbSNP:754739652
1192 1192 c, t dbSNP:751204824
1201 1201 c, t dbSNP:779728345
1206 1206 c, t dbSNP:758011557
1208 1208 a, c, t dbSNP:140964046
1210 1210 a, g dbSNP:764709204
1211 1211 c, g, t dbSNP:767333188
1213 1213 a, t dbSNP:764396178
1215 1215 c, t dbSNP:74315362
1216 1216 a, g dbSNP:761048881
1222 1222 a, c dbSNP:775697482
1223 1223 a, g dbSNP:147089210
1230 1230 a, g dbSNP:548802413
1231 1231 c, t dbSNP:772465237
1232 1232 a, g dbSNP:147034239
1233 1233 a, g dbSNP:759834904
1234 1234 c, t dbSNP:141596564
1236 1236 c, t dbSNP:771080781
1237 1237 c, t dbSNP:748044200
1242 1242 a, g dbSNP:776594413
1250 1250 c, t dbSNP:761407486
1266 1266 a, g dbSNP:766913459
1272 1272 c, t dbSNP:768361068
1273 1273 a, g dbSNP:368784290
1280 1280 a, g dbSNP:375456388
1281 1281 a, g dbSNP:779817999
1284 1284 a, t dbSNP:780985369

Target ORF information:

RefSeq Version XM_011546931
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011546931

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGTCCCGC AGCCCCAGGC CCACACTGAA AGCATGTCGA TCCAGGAGAA CATATCATCC 
CTGCAGCTTC GGTCATGGGT CTCTAAGTCC CAAAGAGACT TAGCAAAGTC CATCCTGATT
GGGGCTCCAG GAGGGCCAGC GGGGTATCTG CGGCGGGCCA GTGTGGCCCA ACTGACCCAG
GAGCTGGGCA CTGCCTTCTT CCAGCAGCAG CAGCTGCCAG CTGCTATGGC AGACACCTTC
CTGGAACACC TCTGCCTACT GGACATTGAC TCCGAGCCCG TGGCTGCTCG CAGTACCAGC
ATCATTGCCA CCATCGGGCC AGCATCTCGC TCCGTGGAGC GCCTCAAGGA GATGATCAAG
GCCGGGATGA ACATTGCGCG ACTCAACTTC TCCCACGGCT CCCACGAGTA CCATGCTGAG
TCCATCGCCA ACGTCCGGGA GGCGGTGGAG AGCTTTGCAG GTTCCCCACT CAGCTACCGG
CCCGTGGCCA TCGCCCTGGA CACCAAGGGA CCGGAGATCC GCACTGGGAT CCTGCAGGGG
GGTCCAGAGT CGGAAGTGGA GCTGGTGAAG GGCTCCCAGG TGCTGGTGAC TGTGGACCCC
GCGTTCCGGA CGCGGGGGAA CGCGAACACC GTGTGGGTGG ACTACCCCAA TATTGTCCGG
GTCGTGCCGG TGGGGGGCCG CATCTACATT GACGACGGGC TCATCTCCCT AGTGGTCCAG
AAAATCGGCC CAGAGGGACT GGTGACCCAA GTGGAGAACG GCGGCGTCCT GGGCAGCCGG
AAGGGCGTGA ACTTGCCAGG GGCCCAGGTG GACTTGCCCG GGCTGTCCGA GCAGGACGTC
CGAGACCTGC GCTTCGGGGT GGAGCATGGG GTGGACATCG TCTTTGCCTC CTTTGTGCGG
AAAGCCAGCG ACGTGGCTGC CGTCAGGGCT GCTCTGGGTC CGGAAGGACA CGGCATCAAG
ATCATCAGCA AAATTGAGAA CCACGAAGGC GTGAAGAGGT TTGATGAAAT CCTGGAGGTG
AGCGACGGCA TCATGGTGGC ACGGGGGGAC CTAGGCATCG AGATCCCAGC AGAGAAGGTT
TTCCTGGCTC AGAAGATGAT GATTGGGCGC TGCAACTTGG CGGGCAAGCC TGTTGTCTGT
GCCACACAGG TCTGGAGTGA GGCCTTGAGA TGCTGGAGAG CATGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu34476D
Sequence Information ORF Nucleotide Sequence (Length: 1533bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pyruvate kinase PKLR isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003315906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCAGACA CCTTCCTGGA ACACCTCTGC CTACTGGACA TTGACTCCGA GCCCGTGGCT 
GCTCGCAGTA CCAGCATCAT TGCCACCATC GGGCCAGCAT CTCGCTCCGT GGAGCGCCTC
AAGGAGATGA TCAAGGCCGG GATGAACATT GCGCGACTCA ACTTCTCCCA CGGCTCCCAC
GAGTACCATG CTGAGTCCAT CGCCAACGTC CGGGAGGCGG TGGAGAGCTT TGCAGGTTCC
CCACTCAGCT ACCGGCCCGT GGCCATCGCC CTGGACACCA AGGGACCGGA GATCCGCACT
GGGATCCTGC AGGGGGGTCC AGAGTCGGAA GTGGAGCTGG TGAAGGGCTC CCAGGTGCTG
GTGACTGTGG ACCCCGCGTT CCGGACGCGG GGGAACGCGA ACACCGTGTG GGTGGACTAC
CCCAATATTG TCCGGGTCGT GCCGGTGGGG GGCCGCATCT ACATTGACGA CGGGCTCATC
TCCCTAGTGG TCCAGAAAAT CGGCCCAGAG GGACTGGTGA CCCAAGTGGA GAACGGCGGC
GTCCTGGGCA GCCGGAAGGG CGTGAACTTG CCAGGGGCCC AGGTGGACTT GCCCGGGCTG
TCCGAGCAGG ACGTCCGAGA CCTGCGCTTC GGGGTGGAGC ATGGGGTGGA CATCGTCTTT
GCCTCCTTTG TGCGGAAAGC CAGCGACGTG GCTGCCGTCA GGGCTGCTCT GGGTCCGGAA
GGACACGGCA TCAAGATCAT CAGCAAAATT GAGAACCACG AAGGCGTGAA GAGGTTTGAT
GAAATCCTGG AGGTGAGCGA CGGCATCATG GTGGCACGGG GGGACCTAGG CATCGAGATC
CCAGCAGAGA AGGTTTTCCT GGCTCAGAAG ATGATGATTG GGCGCTGCAA CTTGGCGGGC
AAGCCTGTTG TCTGTGCCAC ACAGATGCTG GAGAGCATGA TTACCAAGCC CCGGCCAACG
AGGGCAGAGA CAAGCGATGT CGCCAATGCT GTGCTGGATG GGGCTGACTG CATCATGCTG
TCAGGGGAGA CTGCCAAGGG CAACTTCCCT GTGGAAGCGG TGAAGATGCA GCATGCGATT
GCCCGGGAGG CAGAGGCCGC AGTGTACCAC CGGCAGCTGT TTGAGGAGCT ACGTCGGGCA
GCGCCACTAA GCCGTGATCC CACTGAGGTC ACCGCCATTG GTGCTGTGGA GGCTGCCTTC
AAGTGCTGTG CTGCTGCCAT CATTGTGCTG ACCACAACTG GCCGCTCAGC CCAGCTTCTG
TCTCGGTACC GACCTCGGGC AGCAGTCATT GCTGTCACCC GCTCTGCCCA GGCTGCCCGC
CAGGTCCACT TATGCCGAGG AGTCTTCCCC TTGCTTTACC GTGAACCTCC AGAAGCCATC
TGGGCAGATG ATGTAGATCG CCGGGTGCAA TTTGGCATTG AAAGTGGAAA GCTCCGTGGC
TTCCTCCGTG TTGGAGACCT GGTGATTGTG GTGACAGGCT GGCGACCTGG CTCCGGCTAC
ACCAACATCA TGCGGGTGCT AAGCATATCC TGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011545234.1
CDS165..1697
Misc Feature(1)225..1691(+)
Misc Feature(2)228..1682(+)
Misc Feature(3)231..1505(+)
Misc Feature(4)318..1085(+)
Translation
Position Chain Variation Link
5 5 a, g dbSNP:375257968
7 7 c, t dbSNP:746119630
13 13 c, g dbSNP:778939180
14 14 c, t dbSNP:757391113
15 15 c, t dbSNP:753922649
17 17 a, t dbSNP:777978713
21 21 c, t dbSNP:762350074
36 36 a, g dbSNP:372152236
37 37 c, t dbSNP:766209695
40 40 g, t dbSNP:758061395
49 49 c, t dbSNP:137996782
50 50 c, t dbSNP:368867132
54 54 c, g dbSNP:761502347
56 56 c, g dbSNP:776156268
60 60 c, g dbSNP:373541741
63 63 a, g dbSNP:763586752
79 79 c, g, t dbSNP:770019694
80 80 a, g dbSNP:148376707
82 82 a, g, t dbSNP:118204087
83 83 -, g dbSNP:762615239
83 83 a, g, t dbSNP:141341788
87 87 c, t dbSNP:758304847
91 91 g, t dbSNP:745811076
93 93 c, t dbSNP:375189218
94 94 a, g, t dbSNP:374805791
101 101 c, t dbSNP:777368485
103 103 a, t dbSNP:753415917
110 110 a, g dbSNP:370696464
120 120 a, g dbSNP:752199380
122 122 a, g dbSNP:767023470
126 126 g, t dbSNP:760029571
127 127 a, g dbSNP:752093404
129 129 a, t dbSNP:760443294
132 132 a, g dbSNP:753089348
139 139 a, t dbSNP:746308116
141 141 c, t dbSNP:772567442
143 143 a, g dbSNP:200581879
153 153 c, t dbSNP:8177962
165 165 a, g dbSNP:773589949
179 179 c, t dbSNP:770187539
184 184 a, t dbSNP:754696137
188 188 c, t dbSNP:762094018
189 189 c, t dbSNP:200791251
190 190 a, t dbSNP:140571200
195 195 c, t dbSNP:768765063
206 206 c, t dbSNP:745802541
210 210 a, t dbSNP:766051969
212 212 c, t dbSNP:185441545
213 213 a, g dbSNP:139707114
216 216 c, g dbSNP:151005140
218 218 c, t dbSNP:777525158
219 219 a, g dbSNP:368492204
220 220 c, t dbSNP:754785851
228 228 c, g dbSNP:752391774
229 229 a, g dbSNP:375471342
231 231 a, t dbSNP:754454017
244 244 c, t dbSNP:752037743
254 254 c, t dbSNP:147391950
255 255 a, g, t dbSNP:750857114
257 257 a, g dbSNP:759358280
260 260 a, g dbSNP:762937077
267 267 c, t dbSNP:148636159
268 268 a, g dbSNP:534895384
270 270 a, t dbSNP:762661462
272 272 c, g dbSNP:776268137
273 273 g, t dbSNP:143984223
279 279 a, c, t dbSNP:779765565
280 280 a, g dbSNP:371471086
287 287 a, g dbSNP:757921751
306 306 a, g dbSNP:746377052
313 313 c, t dbSNP:779517988
319 319 a, g, t dbSNP:754441130
322 322 g, t dbSNP:754282523
326 326 c, t dbSNP:764337069
340 340 c, g dbSNP:756428691
345 345 c, g dbSNP:752905261
346 346 a, c dbSNP:767598564
357 357 a, g dbSNP:773981429
361 361 a, c dbSNP:118204089
370 370 a, t dbSNP:770337533
371 371 a, c dbSNP:749817706
373 373 a, t dbSNP:574051756
375 375 a, c dbSNP:770175821
387 387 a, g dbSNP:748560715
388 388 a, t dbSNP:757997111
392 392 a, c dbSNP:781504670
404 404 c, t dbSNP:755258394
418 418 a, g dbSNP:757378318
427 427 c, g dbSNP:751716279
432 432 a, g dbSNP:780192373
435 435 c, t dbSNP:758341301
441 441 a, c dbSNP:753840260
446 446 a, g dbSNP:763884887
452 452 a, g dbSNP:146187567
459 459 c, t dbSNP:118204083
470 470 c, t dbSNP:771412921
471 471 c, t dbSNP:767419098
472 472 c, t dbSNP:759323460
477 477 c, g, t dbSNP:199824528
478 478 a, g dbSNP:762330662
479 479 a, g dbSNP:201674983
480 480 a, g dbSNP:779196057
486 486 c, g dbSNP:757359024
495 495 a, g dbSNP:370513342
496 496 c, t dbSNP:781236222
501 501 c, t dbSNP:138482544
504 504 a, g dbSNP:751361992
532 532 c, t dbSNP:370932346
534 534 g, t dbSNP:758042780
537 537 c, g dbSNP:577247981
551 551 a, g dbSNP:764731242
553 553 g, t dbSNP:552916457
557 557 g, t dbSNP:754361540
558 558 a, g dbSNP:781165565
560 560 c, g dbSNP:764631858
562 562 c, t dbSNP:761210207
566 566 a, c dbSNP:775787566
574 574 a, g dbSNP:772473652
587 587 -, c dbSNP:749541053
589 589 a, g dbSNP:759694655
590 590 c, t dbSNP:749833572
592 592 g, t dbSNP:780259014
598 598 a, g dbSNP:200480300
599 599 c, g dbSNP:749332866
603 603 c, g dbSNP:777980472
606 606 c, t dbSNP:768541145
607 607 c, t dbSNP:777667459
608 608 g, t dbSNP:779725913
610 610 c, t dbSNP:200979497
611 611 g, t dbSNP:750102822
617 617 a, c dbSNP:778484808
621 621 a, c dbSNP:756878847
627 627 a, t dbSNP:753384013
628 628 c, t dbSNP:200572803
629 629 c, t dbSNP:756559416
635 635 c, t dbSNP:761145518
638 638 a, g dbSNP:555325288
643 643 a, t dbSNP:768657605
646 646 c, t dbSNP:767781894
647 647 c, t dbSNP:759941126
652 652 a, t dbSNP:774736844
654 654 a, g dbSNP:771329959
658 658 a, g dbSNP:763237413
663 663 a, g dbSNP:764375399
666 666 g, t dbSNP:750606318
671 671 a, g dbSNP:765262843
679 679 c, t dbSNP:769475930
693 693 g, t dbSNP:201953584
694 694 a, t dbSNP:776546824
698 698 c, t dbSNP:772083920
699 699 a, g dbSNP:745797890
702 702 a, g dbSNP:759517847
703 703 a, g dbSNP:774192277
705 705 a, g dbSNP:555712170
717 717 c, t dbSNP:748947397
718 718 a, g dbSNP:368830326
719 719 a, g dbSNP:755602921
723 723 a, g dbSNP:267598065
728 728 c, g dbSNP:747744073
731 731 c, t dbSNP:780539049
737 737 a, g dbSNP:755610815
741 741 a, g dbSNP:752053420
742 742 c, t dbSNP:766853199
747 747 a, g dbSNP:750161685
750 750 a, c, g dbSNP:140575553
752 752 a, c dbSNP:765541284
755 755 g, t dbSNP:761905811
758 758 c, t dbSNP:148319141
762 762 c, t dbSNP:199959447
766 766 c, g dbSNP:769783555
771 771 a, c dbSNP:764143249
776 776 c, t dbSNP:576755609
777 777 g, t dbSNP:200695047
778 778 c, t dbSNP:770747923
780 780 a, c, g dbSNP:772860949
786 786 c, g dbSNP:147659527
789 789 c, t dbSNP:747753310
790 790 a, g dbSNP:141393570
795 795 a, c, g dbSNP:747549978
798 798 a, g dbSNP:780659178
798 798 -, g dbSNP:754939638
801 801 a, g dbSNP:147689373
806 806 c, t dbSNP:750836008
808 808 a, g, t dbSNP:758560647
809 809 c, g dbSNP:757554998
818 818 -, c dbSNP:751449001
830 830 a, c dbSNP:150532071
838 838 a, g dbSNP:749666187
841 841 a, g dbSNP:780875123
846 846 a, t dbSNP:749573131
852 852 g, t dbSNP:760709075
855 855 a, g dbSNP:751524706
856 856 c, t dbSNP:766353400
861 861 a, c, g dbSNP:148435198
868 868 a, c dbSNP:769464933
875 875 a, g dbSNP:761529212
878 878 c, t dbSNP:776382179
879 879 c, g dbSNP:201979697
880 880 c, t dbSNP:746566827
881 881 c, g dbSNP:200840994
890 890 a, c, t dbSNP:141571402
891 891 a, c, g dbSNP:373990660
892 892 g, t dbSNP:754124936
909 909 a, c dbSNP:777926506
911 911 a, g dbSNP:756311123
923 923 a, c, t dbSNP:745663677
925 925 a, g dbSNP:767580478
928 928 a, g dbSNP:763006858
930 930 c, g dbSNP:549295725
938 938 g, t dbSNP:763964110
943 943 a, g dbSNP:760413736
944 944 c, t dbSNP:8177974
951 951 c, g dbSNP:767088632
952 952 a, t dbSNP:758924903
963 963 a, g dbSNP:773893686
965 965 a, c, t dbSNP:138476691
966 966 a, g dbSNP:773626254
974 974 a, g dbSNP:756339496
981 981 c, g, t dbSNP:748376799
983 983 c, g dbSNP:781220573
987 987 a, g, t dbSNP:747097960
992 992 a, g dbSNP:780173822
995 995 c, g dbSNP:757318290
998 998 c, t dbSNP:376694187
999 999 a, g dbSNP:781715321
1014 1014 -, aag dbSNP:762301937
1016 1016 a, g dbSNP:763982504
1021 1021 a, t dbSNP:755934816
1039 1039 c, t dbSNP:752423472
1042 1042 c, t dbSNP:779152555
1047 1047 c, t dbSNP:138871700
1051 1051 c, g dbSNP:773803500
1053 1053 a, g dbSNP:765903674
1060 1060 a, c dbSNP:763466930
1061 1061 a, g dbSNP:773644908
1068 1068 c, g, t dbSNP:533316562
1071 1071 g, t dbSNP:777020292
1076 1076 -, ctgt dbSNP:749942178
1094 1094 a, g dbSNP:754739652
1100 1100 c, t dbSNP:751204824
1109 1109 c, t dbSNP:779728345
1114 1114 c, t dbSNP:758011557
1116 1116 a, c, t dbSNP:140964046
1118 1118 a, g dbSNP:764709204
1119 1119 c, g, t dbSNP:767333188
1121 1121 a, t dbSNP:764396178
1123 1123 c, t dbSNP:74315362
1124 1124 a, g dbSNP:761048881
1130 1130 a, c dbSNP:775697482
1131 1131 a, g dbSNP:147089210
1138 1138 a, g dbSNP:548802413
1139 1139 c, t dbSNP:772465237
1140 1140 a, g dbSNP:147034239
1141 1141 a, g dbSNP:759834904
1142 1142 c, t dbSNP:141596564
1144 1144 c, t dbSNP:771080781
1145 1145 c, t dbSNP:748044200
1150 1150 a, g dbSNP:776594413
1158 1158 c, t dbSNP:761407486
1174 1174 a, g dbSNP:766913459
1180 1180 c, t dbSNP:768361068
1181 1181 a, g dbSNP:368784290
1188 1188 a, g dbSNP:375456388
1189 1189 a, g dbSNP:779817999
1192 1192 a, t dbSNP:780985369
1204 1204 a, c, g dbSNP:745461639
1207 1207 a, c dbSNP:774006353
1208 1208 c, t dbSNP:756750467
1211 1211 c, t dbSNP:754321902
1218 1218 a, g dbSNP:764713394
1222 1222 c, t dbSNP:756549612
1223 1223 a, g, t dbSNP:767981692
1224 1224 a, g dbSNP:759855502
1233 1233 a, c dbSNP:118204084
1240 1240 a, c dbSNP:763528176
1241 1241 a, g dbSNP:774652817
1242 1242 -, a dbSNP:751363828
1245 1245 a, g dbSNP:756703156
1246 1246 a, c dbSNP:753198136
1249 1249 a, g dbSNP:768002493
1262 1262 c, t dbSNP:755357782
1263 1263 a, g dbSNP:762591322
1275 1275 c, t dbSNP:142238268
1278 1278 c, t dbSNP:778805401
1279 1279 a, g dbSNP:758965704
1290 1290 a, g, t dbSNP:771145576
1296 1296 c, t dbSNP:773396824
1297 1297 a, g dbSNP:370554398
1300 1300 a, g dbSNP:760606711
1306 1306 c, t dbSNP:531294009
1307 1307 a, g dbSNP:771876200
1311 1311 a, c, t dbSNP:774102445
1317 1317 c, t dbSNP:189360283
1318 1318 a, g dbSNP:149932430
1325 1325 c, t dbSNP:777357253
1330 1330 a, c dbSNP:769123407
1335 1335 a, g dbSNP:748700869
1337 1337 c, t dbSNP:146708702
1338 1338 a, g dbSNP:756864028
1341 1341 a, g dbSNP:377022809
1345 1345 a, g dbSNP:755522396
1350 1350 a, g dbSNP:752034960
1351 1351 c, t dbSNP:149946271
1355 1355 a, g dbSNP:758655787
1375 1375 c, g dbSNP:750540943
1380 1380 g, t dbSNP:765388322
1385 1385 a, c dbSNP:761831271
1392 1392 c, t dbSNP:753890733
1394 1394 c, g dbSNP:572233729
1401 1401 a, g dbSNP:759466273
1406 1406 a, c dbSNP:774262110
1407 1407 c, t dbSNP:201406712
1408 1408 a, g dbSNP:118204085
1414 1414 c, t dbSNP:773040738
1418 1418 a, g dbSNP:765024950
1421 1421 g, t dbSNP:761542089
1428 1428 c, t dbSNP:116100695
1429 1429 a, g dbSNP:768210809
1435 1435 a, g dbSNP:369183199
1440 1440 c, t dbSNP:200133000
1441 1441 a, g, t dbSNP:746257586
1448 1448 a, g, t dbSNP:757534925
1450 1450 a, t dbSNP:749456521
1456 1456 c, t dbSNP:141560532
1463 1463 c, t dbSNP:756147719
1464 1464 a, c, t dbSNP:551883218
1465 1465 a, g dbSNP:758327704
1480 1480 c, t dbSNP:750255401
1483 1483 g, t dbSNP:185753709
1484 1484 a, c dbSNP:761558916
1488 1488 a, g dbSNP:8177988
1490 1490 c, t dbSNP:376065116
1491 1491 a, c dbSNP:760152160
1493 1493 c, t dbSNP:201011399
1497 1497 c, t dbSNP:760208078
1501 1501 a, g dbSNP:113403872
1511 1511 c, t dbSNP:371489505
1519 1519 c, t dbSNP:779681839
1524 1524 c, t dbSNP:139002629
1525 1525 a, g dbSNP:771218326
1526 1526 a, t dbSNP:749544468
1529 1529 a, c dbSNP:761819694
1530 1530 a, c, t dbSNP:562318137
1533 1533 c, t dbSNP:756341032
1551 1551 a, g dbSNP:748219831
1566 1566 c, t dbSNP:201255024
1567 1567 a, g dbSNP:758278200
1568 1568 c, g dbSNP:750305814
1571 1571 g, t dbSNP:192697407
1576 1576 c, t dbSNP:754526647
1580 1580 c, t dbSNP:757065547
1586 1586 a, t dbSNP:201217064
1590 1590 g, t dbSNP:763878359
1600 1600 a, g dbSNP:752328700
1601 1601 c, t dbSNP:766970429
1603 1603 g, t dbSNP:759039445
1611 1611 c, t dbSNP:751050425
1612 1612 a, g dbSNP:375525211
1617 1617 a, g dbSNP:190966689
1626 1626 a, g dbSNP:370316462
1627 1627 c, t dbSNP:773628430
1635 1635 g, t dbSNP:770125793
1645 1645 a, g dbSNP:370938255
1647 1647 c, t dbSNP:532230312
1648 1648 -, gacctggc dbSNP:771142025
1658 1658 a, c, t dbSNP:140859641
1671 1671 a, t dbSNP:780275059
1673 1673 c, t dbSNP:771049942
1675 1675 c, t dbSNP:749179500
1676 1676 a, g dbSNP:777435169
1677 1677 a, c, t dbSNP:1052176
1678 1678 a, g dbSNP:61755431
1682 1682 c, g dbSNP:552203379
1685 1685 a, g dbSNP:761092859
1700 1700 a, c, t dbSNP:368150636
1701 1701 a, g dbSNP:765864419
1709 1709 a, c dbSNP:758922618
1710 1710 c, g, t dbSNP:1052177
1711 1711 c, g, t dbSNP:8177994
1712 1712 c, g, t dbSNP:374751259
1717 1717 a, g dbSNP:775724792
1719 1719 a, c dbSNP:772210964
1731 1731 c, t dbSNP:749269783
1735 1735 c, t dbSNP:773153185
1754 1754 a, g dbSNP:569710209
1759 1759 c, t dbSNP:542249964
1769 1769 a, c dbSNP:530076782
1775 1775 c, t dbSNP:563189341
1778 1778 c, t dbSNP:186058494
1782 1782 a, g dbSNP:768976043
1833 1833 a, g dbSNP:762039790
1860 1860 c, t dbSNP:774442850
1862 1862 a, g dbSNP:772906215
1889 1889 a, t dbSNP:577392110
1897 1897 a, t dbSNP:768777565
1906 1906 -, g dbSNP:35957800
1953 1953 a, g dbSNP:749428064
1964 1964 c, t dbSNP:932972
1978 1978 g, t dbSNP:770894965

Target ORF information:

RefSeq Version XM_011546932
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011546932

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCAGACA CCTTCCTGGA ACACCTCTGC CTACTGGACA TTGACTCCGA GCCCGTGGCT 
GCTCGCAGTA CCAGCATCAT TGCCACCATC GGGCCAGCAT CTCGCTCCGT GGAGCGCCTC
AAGGAGATGA TCAAGGCCGG GATGAACATT GCGCGACTCA ACTTCTCCCA CGGCTCCCAC
GAGTACCATG CTGAGTCCAT CGCCAACGTC CGGGAGGCGG TGGAGAGCTT TGCAGGTTCC
CCACTCAGCT ACCGGCCCGT GGCCATCGCC CTGGACACCA AGGGACCGGA GATCCGCACT
GGGATCCTGC AGGGGGGTCC AGAGTCGGAA GTGGAGCTGG TGAAGGGCTC CCAGGTGCTG
GTGACTGTGG ACCCCGCGTT CCGGACGCGG GGGAACGCGA ACACCGTGTG GGTGGACTAC
CCCAATATTG TCCGGGTCGT GCCGGTGGGG GGCCGCATCT ACATTGACGA CGGGCTCATC
TCCCTAGTGG TCCAGAAAAT CGGCCCAGAG GGACTGGTGA CCCAAGTGGA GAACGGCGGC
GTCCTGGGCA GCCGGAAGGG CGTGAACTTG CCAGGGGCCC AGGTGGACTT GCCCGGGCTG
TCCGAGCAGG ACGTCCGAGA CCTGCGCTTC GGGGTGGAGC ATGGGGTGGA CATCGTCTTT
GCCTCCTTTG TGCGGAAAGC CAGCGACGTG GCTGCCGTCA GGGCTGCTCT GGGTCCGGAA
GGACACGGCA TCAAGATCAT CAGCAAAATT GAGAACCACG AAGGCGTGAA GAGGTTTGAT
GAAATCCTGG AGGTGAGCGA CGGCATCATG GTGGCACGGG GGGACCTAGG CATCGAGATC
CCAGCAGAGA AGGTTTTCCT GGCTCAGAAG ATGATGATTG GGCGCTGCAA CTTGGCGGGC
AAGCCTGTTG TCTGTGCCAC ACAGATGCTG GAGAGCATGA TTACCAAGCC CCGGCCAACG
AGGGCAGAGA CAAGCGATGT CGCCAATGCT GTGCTGGATG GGGCTGACTG CATCATGCTG
TCAGGGGAGA CTGCCAAGGG CAACTTCCCT GTGGAAGCGG TGAAGATGCA GCATGCGATT
GCCCGGGAGG CAGAGGCCGC AGTGTACCAC CGGCAGCTGT TTGAGGAGCT ACGTCGGGCA
GCGCCACTAA GCCGTGATCC CACTGAGGTC ACCGCCATTG GTGCTGTGGA GGCTGCCTTC
AAGTGCTGTG CTGCTGCCAT CATTGTGCTG ACCACAACTG GCCGCTCAGC CCAGCTTCTG
TCTCGGTACC GACCTCGGGC AGCAGTCATT GCTGTCACCC GCTCTGCCCA GGCTGCCCGC
CAGGTCCACT TATGCCGAGG AGTCTTCCCC TTGCTTTACC GTGAACCTCC AGAAGCCATC
TGGGCAGATG ATGTAGATCG CCGGGTGCAA TTTGGCATTG AAAGTGGAAA GCTCCGTGGC
TTCCTCCGTG TTGGAGACCT GGTGATTGTG GTGACAGGCT GGCGACCTGG CTCCGGCTAC
ACCAACATCA TGCGGGTGCT AAGCATATCC TGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu34475D
Sequence Information ORF Nucleotide Sequence (Length: 1884bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pyruvate kinase PKLR isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578801112. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGAAGGGC TGAAGTCTAG AGGTCGCGGC TGGAGGCGTC AGTGCCGGAA GCGAGCCAGG 
CTGGGAACGT CCCCGCTGTG GCTTTGGCTG CCTGTCTTAC TCAAGTTTTC ATTATCTTCC
TGGGCAGCTG ATTGGCACCC CCAGGCCCAC ACTGAAAGCA TGTCGATCCA GGAGAACATA
TCATCCCTGC AGCTTCGGTC ATGGGTCTCT AAGTCCCAAA GAGACTTAGC AAAGTCCATC
CTGATTGGGG CTCCAGGAGG GCCAGCGGGG TATCTGCGGC GGGCCAGTGT GGCCCAACTG
ACCCAGGAGC TGGGCACTGC CTTCTTCCAG CAGCAGCAGC TGCCAGCTGC TATGGCAGAC
ACCTTCCTGG AACACCTCTG CCTACTGGAC ATTGACTCCG AGCCCGTGGC TGCTCGCAGT
ACCAGCATCA TTGCCACCAT CGGGCCAGCA TCTCGCTCCG TGGAGCGCCT CAAGGAGATG
ATCAAGGCCG GGATGAACAT TGCGCGACTC AACTTCTCCC ACGGCTCCCA CGAGTACCAT
GCTGAGTCCA TCGCCAACGT CCGGGAGGCG GTGGAGAGCT TTGCAGGTTC CCCACTCAGC
TACCGGCCCG TGGCCATCGC CCTGGACACC AAGGGACCGG AGATCCGCAC TGGGATCCTG
CAGGGGGGTC CAGAGTCGGA AGTGGAGCTG GTGAAGGGCT CCCAGGTGCT GGTGACTGTG
GACCCCGCGT TCCGGACGCG GGGGAACGCG AACACCGTGT GGGTGGACTA CCCCAATATT
GTCCGGGTCG TGCCGGTGGG GGGCCGCATC TACATTGACG ACGGGCTCAT CTCCCTAGTG
GTCCAGAAAA TCGGCCCAGA GGGACTGGTG ACCCAAGTGG AGAACGGCGG CGTCCTGGGC
AGCCGGAAGG GCGTGAACTT GCCAGGGGCC CAGGTGGACT TGCCCGGGCT GTCCGAGCAG
GACGTCCGAG ACCTGCGCTT CGGGGTGGAG CATGGGGTGG ACATCGTCTT TGCCTCCTTT
GTGCGGAAAG CCAGCGACGT GGCTGCCGTC AGGGCTGCTC TGGGTCCGGA AGGACACGGC
ATCAAGATCA TCAGCAAAAT TGAGAACCAC GAAGGCGTGA AGAGGTTTGA TGAAATCCTG
GAGGTGAGCG ACGGCATCAT GGTGGCACGG GGGGACCTAG GCATCGAGAT CCCAGCAGAG
AAGGTTTTCC TGGCTCAGAA GATGATGATT GGGCGCTGCA ACTTGGCGGG CAAGCCTGTT
GTCTGTGCCA CACAGATGCT GGAGAGCATG ATTACCAAGC CCCGGCCAAC GAGGGCAGAG
ACAAGCGATG TCGCCAATGC TGTGCTGGAT GGGGCTGACT GCATCATGCT GTCAGGGGAG
ACTGCCAAGG GCAACTTCCC TGTGGAAGCG GTGAAGATGC AGCATGCGAT TGCCCGGGAG
GCAGAGGCCG CAGTGTACCA CCGGCAGCTG TTTGAGGAGC TACGTCGGGC AGCGCCACTA
AGCCGTGATC CCACTGAGGT CACCGCCATT GGTGCTGTGG AGGCTGCCTT CAAGTGCTGT
GCTGCTGCCA TCATTGTGCT GACCACAACT GGCCGCTCAG CCCAGCTTCT GTCTCGGTAC
CGACCTCGGG CAGCAGTCAT TGCTGTCACC CGCTCTGCCC AGGCTGCCCG CCAGGTCCAC
TTATGCCGAG GAGTCTTCCC CTTGCTTTAC CGTGAACCTC CAGAAGCCAT CTGGGCAGAT
GATGTAGATC GCCGGGTGCA ATTTGGCATT GAAAGTGGAA AGCTCCGTGG CTTCCTCCGT
GTTGGAGACC TGGTGATTGT GGTGACAGGC TGGCGACCTG GCTCCGGCTA CACCAACATC
ATGCGGGTGC TAAGCATATC CTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005245323.1
CDS19..1902
Misc Feature(1)430..1896(+)
Misc Feature(2)433..1887(+)
Misc Feature(3)436..1710(+)
Misc Feature(4)523..1290(+)
Translation
Position Chain Variation Link
60 60 a, g dbSNP:12043597
64 64 a, c dbSNP:754038530
65 65 a, g dbSNP:761345776
93 93 a, g dbSNP:573445586
150 150 c, t dbSNP:766696826
157 157 c, t dbSNP:751786807
159 159 c, t dbSNP:766550230
160 160 c, g dbSNP:758498340
165 165 c, t dbSNP:750568645
169 169 a, g dbSNP:765203859
171 171 c, t dbSNP:760728696
176 176 a, c, g, t dbSNP:201169481
182 182 a, c dbSNP:774176341
183 183 a, g dbSNP:139697646
189 189 g, t dbSNP:758112712
197 197 c, t dbSNP:762490269
214 214 c, t dbSNP:772818969
215 215 a, g dbSNP:769174570
220 220 c, t dbSNP:748703432
222 222 a, g dbSNP:750812851
229 229 a, g dbSNP:781610938
233 233 c, g dbSNP:769038526
236 236 a, g dbSNP:200357340
247 247 a, g dbSNP:754783713
254 254 c, g dbSNP:747323509
260 260 c, t dbSNP:780370877
263 263 c, t dbSNP:142735355
266 266 a, g dbSNP:771523060
269 269 c, t dbSNP:150077703
284 284 c, g, t dbSNP:770019694
285 285 a, g dbSNP:148376707
287 287 a, g, t dbSNP:118204087
288 288 -, g dbSNP:762615239
288 288 a, g, t dbSNP:141341788
292 292 c, t dbSNP:758304847
296 296 g, t dbSNP:745811076
298 298 c, t dbSNP:375189218
299 299 a, g, t dbSNP:374805791
306 306 c, t dbSNP:777368485
308 308 a, t dbSNP:753415917
315 315 a, g dbSNP:370696464
325 325 a, g dbSNP:752199380
327 327 a, g dbSNP:767023470
331 331 g, t dbSNP:760029571
332 332 a, g dbSNP:752093404
334 334 a, t dbSNP:760443294
337 337 a, g dbSNP:753089348
344 344 a, t dbSNP:746308116
346 346 c, t dbSNP:772567442
348 348 a, g dbSNP:200581879
358 358 c, t dbSNP:8177962
370 370 a, g dbSNP:773589949
384 384 c, t dbSNP:770187539
389 389 a, t dbSNP:754696137
393 393 c, t dbSNP:762094018
394 394 c, t dbSNP:200791251
395 395 a, t dbSNP:140571200
400 400 c, t dbSNP:768765063
411 411 c, t dbSNP:745802541
415 415 a, t dbSNP:766051969
417 417 c, t dbSNP:185441545
418 418 a, g dbSNP:139707114
421 421 c, g dbSNP:151005140
423 423 c, t dbSNP:777525158
424 424 a, g dbSNP:368492204
425 425 c, t dbSNP:754785851
433 433 c, g dbSNP:752391774
434 434 a, g dbSNP:375471342
436 436 a, t dbSNP:754454017
449 449 c, t dbSNP:752037743
459 459 c, t dbSNP:147391950
460 460 a, g, t dbSNP:750857114
462 462 a, g dbSNP:759358280
465 465 a, g dbSNP:762937077
472 472 c, t dbSNP:148636159
473 473 a, g dbSNP:534895384
475 475 a, t dbSNP:762661462
477 477 c, g dbSNP:776268137
478 478 g, t dbSNP:143984223
484 484 a, c, t dbSNP:779765565
485 485 a, g dbSNP:371471086
492 492 a, g dbSNP:757921751
511 511 a, g dbSNP:746377052
518 518 c, t dbSNP:779517988
524 524 a, g, t dbSNP:754441130
527 527 g, t dbSNP:754282523
531 531 c, t dbSNP:764337069
545 545 c, g dbSNP:756428691
550 550 c, g dbSNP:752905261
551 551 a, c dbSNP:767598564
562 562 a, g dbSNP:773981429
566 566 a, c dbSNP:118204089
575 575 a, t dbSNP:770337533
576 576 a, c dbSNP:749817706
578 578 a, t dbSNP:574051756
580 580 a, c dbSNP:770175821
592 592 a, g dbSNP:748560715
593 593 a, t dbSNP:757997111
597 597 a, c dbSNP:781504670
609 609 c, t dbSNP:755258394
623 623 a, g dbSNP:757378318
632 632 c, g dbSNP:751716279
637 637 a, g dbSNP:780192373
640 640 c, t dbSNP:758341301
646 646 a, c dbSNP:753840260
651 651 a, g dbSNP:763884887
657 657 a, g dbSNP:146187567
664 664 c, t dbSNP:118204083
675 675 c, t dbSNP:771412921
676 676 c, t dbSNP:767419098
677 677 c, t dbSNP:759323460
682 682 c, g, t dbSNP:199824528
683 683 a, g dbSNP:762330662
684 684 a, g dbSNP:201674983
685 685 a, g dbSNP:779196057
691 691 c, g dbSNP:757359024
700 700 a, g dbSNP:370513342
701 701 c, t dbSNP:781236222
706 706 c, t dbSNP:138482544
709 709 a, g dbSNP:751361992
737 737 c, t dbSNP:370932346
739 739 g, t dbSNP:758042780
742 742 c, g dbSNP:577247981
756 756 a, g dbSNP:764731242
758 758 g, t dbSNP:552916457
762 762 g, t dbSNP:754361540
763 763 a, g dbSNP:781165565
765 765 c, g dbSNP:764631858
767 767 c, t dbSNP:761210207
771 771 a, c dbSNP:775787566
779 779 a, g dbSNP:772473652
792 792 -, c dbSNP:749541053
794 794 a, g dbSNP:759694655
795 795 c, t dbSNP:749833572
797 797 g, t dbSNP:780259014
803 803 a, g dbSNP:200480300
804 804 c, g dbSNP:749332866
808 808 c, g dbSNP:777980472
811 811 c, t dbSNP:768541145
812 812 c, t dbSNP:777667459
813 813 g, t dbSNP:779725913
815 815 c, t dbSNP:200979497
816 816 g, t dbSNP:750102822
822 822 a, c dbSNP:778484808
826 826 a, c dbSNP:756878847
832 832 a, t dbSNP:753384013
833 833 c, t dbSNP:200572803
834 834 c, t dbSNP:756559416
840 840 c, t dbSNP:761145518
843 843 a, g dbSNP:555325288
848 848 a, t dbSNP:768657605
851 851 c, t dbSNP:767781894
852 852 c, t dbSNP:759941126
857 857 a, t dbSNP:774736844
859 859 a, g dbSNP:771329959
863 863 a, g dbSNP:763237413
868 868 a, g dbSNP:764375399
871 871 g, t dbSNP:750606318
876 876 a, g dbSNP:765262843
884 884 c, t dbSNP:769475930
898 898 g, t dbSNP:201953584
899 899 a, t dbSNP:776546824
903 903 c, t dbSNP:772083920
904 904 a, g dbSNP:745797890
907 907 a, g dbSNP:759517847
908 908 a, g dbSNP:774192277
910 910 a, g dbSNP:555712170
922 922 c, t dbSNP:748947397
923 923 a, g dbSNP:368830326
924 924 a, g dbSNP:755602921
928 928 a, g dbSNP:267598065
933 933 c, g dbSNP:747744073
936 936 c, t dbSNP:780539049
942 942 a, g dbSNP:755610815
946 946 a, g dbSNP:752053420
947 947 c, t dbSNP:766853199
952 952 a, g dbSNP:750161685
955 955 a, c, g dbSNP:140575553
957 957 a, c dbSNP:765541284
960 960 g, t dbSNP:761905811
963 963 c, t dbSNP:148319141
967 967 c, t dbSNP:199959447
971 971 c, g dbSNP:769783555
976 976 a, c dbSNP:764143249
981 981 c, t dbSNP:576755609
982 982 g, t dbSNP:200695047
983 983 c, t dbSNP:770747923
985 985 a, c, g dbSNP:772860949
991 991 c, g dbSNP:147659527
994 994 c, t dbSNP:747753310
995 995 a, g dbSNP:141393570
1000 1000 a, c, g dbSNP:747549978
1003 1003 a, g dbSNP:780659178
1003 1003 -, g dbSNP:754939638
1006 1006 a, g dbSNP:147689373
1011 1011 c, t dbSNP:750836008
1013 1013 a, g, t dbSNP:758560647
1014 1014 c, g dbSNP:757554998
1023 1023 -, c dbSNP:751449001
1035 1035 a, c dbSNP:150532071
1043 1043 a, g dbSNP:749666187
1046 1046 a, g dbSNP:780875123
1051 1051 a, t dbSNP:749573131
1057 1057 g, t dbSNP:760709075
1060 1060 a, g dbSNP:751524706
1061 1061 c, t dbSNP:766353400
1066 1066 a, c, g dbSNP:148435198
1073 1073 a, c dbSNP:769464933
1080 1080 a, g dbSNP:761529212
1083 1083 c, t dbSNP:776382179
1084 1084 c, g dbSNP:201979697
1085 1085 c, t dbSNP:746566827
1086 1086 c, g dbSNP:200840994
1095 1095 a, c, t dbSNP:141571402
1096 1096 a, c, g dbSNP:373990660
1097 1097 g, t dbSNP:754124936
1114 1114 a, c dbSNP:777926506
1116 1116 a, g dbSNP:756311123
1128 1128 a, c, t dbSNP:745663677
1130 1130 a, g dbSNP:767580478
1133 1133 a, g dbSNP:763006858
1135 1135 c, g dbSNP:549295725
1143 1143 g, t dbSNP:763964110
1148 1148 a, g dbSNP:760413736
1149 1149 c, t dbSNP:8177974
1156 1156 c, g dbSNP:767088632
1157 1157 a, t dbSNP:758924903
1168 1168 a, g dbSNP:773893686
1170 1170 a, c, t dbSNP:138476691
1171 1171 a, g dbSNP:773626254
1179 1179 a, g dbSNP:756339496
1186 1186 c, g, t dbSNP:748376799
1188 1188 c, g dbSNP:781220573
1192 1192 a, g, t dbSNP:747097960
1197 1197 a, g dbSNP:780173822
1200 1200 c, g dbSNP:757318290
1203 1203 c, t dbSNP:376694187
1204 1204 a, g dbSNP:781715321
1219 1219 -, aag dbSNP:762301937
1221 1221 a, g dbSNP:763982504
1226 1226 a, t dbSNP:755934816
1244 1244 c, t dbSNP:752423472
1247 1247 c, t dbSNP:779152555
1252 1252 c, t dbSNP:138871700
1256 1256 c, g dbSNP:773803500
1258 1258 a, g dbSNP:765903674
1265 1265 a, c dbSNP:763466930
1266 1266 a, g dbSNP:773644908
1273 1273 c, g, t dbSNP:533316562
1276 1276 g, t dbSNP:777020292
1281 1281 -, ctgt dbSNP:749942178
1299 1299 a, g dbSNP:754739652
1305 1305 c, t dbSNP:751204824
1314 1314 c, t dbSNP:779728345
1319 1319 c, t dbSNP:758011557
1321 1321 a, c, t dbSNP:140964046
1323 1323 a, g dbSNP:764709204
1324 1324 c, g, t dbSNP:767333188
1326 1326 a, t dbSNP:764396178
1328 1328 c, t dbSNP:74315362
1329 1329 a, g dbSNP:761048881
1335 1335 a, c dbSNP:775697482
1336 1336 a, g dbSNP:147089210
1343 1343 a, g dbSNP:548802413
1344 1344 c, t dbSNP:772465237
1345 1345 a, g dbSNP:147034239
1346 1346 a, g dbSNP:759834904
1347 1347 c, t dbSNP:141596564
1349 1349 c, t dbSNP:771080781
1350 1350 c, t dbSNP:748044200
1355 1355 a, g dbSNP:776594413
1363 1363 c, t dbSNP:761407486
1379 1379 a, g dbSNP:766913459
1385 1385 c, t dbSNP:768361068
1386 1386 a, g dbSNP:368784290
1393 1393 a, g dbSNP:375456388
1394 1394 a, g dbSNP:779817999
1397 1397 a, t dbSNP:780985369
1409 1409 a, c, g dbSNP:745461639
1412 1412 a, c dbSNP:774006353
1413 1413 c, t dbSNP:756750467
1416 1416 c, t dbSNP:754321902
1423 1423 a, g dbSNP:764713394
1427 1427 c, t dbSNP:756549612
1428 1428 a, g, t dbSNP:767981692
1429 1429 a, g dbSNP:759855502
1438 1438 a, c dbSNP:118204084
1445 1445 a, c dbSNP:763528176
1446 1446 a, g dbSNP:774652817
1447 1447 -, a dbSNP:751363828
1450 1450 a, g dbSNP:756703156
1451 1451 a, c dbSNP:753198136
1454 1454 a, g dbSNP:768002493
1467 1467 c, t dbSNP:755357782
1468 1468 a, g dbSNP:762591322
1480 1480 c, t dbSNP:142238268
1483 1483 c, t dbSNP:778805401
1484 1484 a, g dbSNP:758965704
1495 1495 a, g, t dbSNP:771145576
1501 1501 c, t dbSNP:773396824
1502 1502 a, g dbSNP:370554398
1505 1505 a, g dbSNP:760606711
1511 1511 c, t dbSNP:531294009
1512 1512 a, g dbSNP:771876200
1516 1516 a, c, t dbSNP:774102445
1522 1522 c, t dbSNP:189360283
1523 1523 a, g dbSNP:149932430
1530 1530 c, t dbSNP:777357253
1535 1535 a, c dbSNP:769123407
1540 1540 a, g dbSNP:748700869
1542 1542 c, t dbSNP:146708702
1543 1543 a, g dbSNP:756864028
1546 1546 a, g dbSNP:377022809
1550 1550 a, g dbSNP:755522396
1555 1555 a, g dbSNP:752034960
1556 1556 c, t dbSNP:149946271
1560 1560 a, g dbSNP:758655787
1580 1580 c, g dbSNP:750540943
1585 1585 g, t dbSNP:765388322
1590 1590 a, c dbSNP:761831271
1597 1597 c, t dbSNP:753890733
1599 1599 c, g dbSNP:572233729
1606 1606 a, g dbSNP:759466273
1611 1611 a, c dbSNP:774262110
1612 1612 c, t dbSNP:201406712
1613 1613 a, g dbSNP:118204085
1619 1619 c, t dbSNP:773040738
1623 1623 a, g dbSNP:765024950
1626 1626 g, t dbSNP:761542089
1633 1633 c, t dbSNP:116100695
1634 1634 a, g dbSNP:768210809
1640 1640 a, g dbSNP:369183199
1645 1645 c, t dbSNP:200133000
1646 1646 a, g, t dbSNP:746257586
1653 1653 a, g, t dbSNP:757534925
1655 1655 a, t dbSNP:749456521
1661 1661 c, t dbSNP:141560532
1668 1668 c, t dbSNP:756147719
1669 1669 a, c, t dbSNP:551883218
1670 1670 a, g dbSNP:758327704
1685 1685 c, t dbSNP:750255401
1688 1688 g, t dbSNP:185753709
1689 1689 a, c dbSNP:761558916
1693 1693 a, g dbSNP:8177988
1695 1695 c, t dbSNP:376065116
1696 1696 a, c dbSNP:760152160
1698 1698 c, t dbSNP:201011399
1702 1702 c, t dbSNP:760208078
1706 1706 a, g dbSNP:113403872
1716 1716 c, t dbSNP:371489505
1724 1724 c, t dbSNP:779681839
1729 1729 c, t dbSNP:139002629
1730 1730 a, g dbSNP:771218326
1731 1731 a, t dbSNP:749544468
1734 1734 a, c dbSNP:761819694
1735 1735 a, c, t dbSNP:562318137
1738 1738 c, t dbSNP:756341032
1756 1756 a, g dbSNP:748219831
1771 1771 c, t dbSNP:201255024
1772 1772 a, g dbSNP:758278200
1773 1773 c, g dbSNP:750305814
1776 1776 g, t dbSNP:192697407
1781 1781 c, t dbSNP:754526647
1785 1785 c, t dbSNP:757065547
1791 1791 a, t dbSNP:201217064
1795 1795 g, t dbSNP:763878359
1805 1805 a, g dbSNP:752328700
1806 1806 c, t dbSNP:766970429
1808 1808 g, t dbSNP:759039445
1816 1816 c, t dbSNP:751050425
1817 1817 a, g dbSNP:375525211
1822 1822 a, g dbSNP:190966689
1831 1831 a, g dbSNP:370316462
1832 1832 c, t dbSNP:773628430
1840 1840 g, t dbSNP:770125793
1850 1850 a, g dbSNP:370938255
1852 1852 c, t dbSNP:532230312
1853 1853 -, gacctggc dbSNP:771142025
1863 1863 a, c, t dbSNP:140859641
1876 1876 a, t dbSNP:780275059
1878 1878 c, t dbSNP:771049942
1880 1880 c, t dbSNP:749179500
1881 1881 a, g dbSNP:777435169
1882 1882 a, c, t dbSNP:1052176
1883 1883 a, g dbSNP:61755431
1887 1887 c, g dbSNP:552203379
1890 1890 a, g dbSNP:761092859
1905 1905 a, c, t dbSNP:368150636
1906 1906 a, g dbSNP:765864419
1914 1914 a, c dbSNP:758922618
1915 1915 c, g, t dbSNP:1052177
1916 1916 c, g, t dbSNP:8177994
1917 1917 c, g, t dbSNP:374751259
1922 1922 a, g dbSNP:775724792
1924 1924 a, c dbSNP:772210964
1936 1936 c, t dbSNP:749269783
1940 1940 c, t dbSNP:773153185
1959 1959 a, g dbSNP:569710209
1964 1964 c, t dbSNP:542249964
1974 1974 a, c dbSNP:530076782
1980 1980 c, t dbSNP:563189341
1983 1983 c, t dbSNP:186058494
1987 1987 a, g dbSNP:768976043
2038 2038 a, g dbSNP:762039790
2065 2065 c, t dbSNP:774442850
2067 2067 a, g dbSNP:772906215
2094 2094 a, t dbSNP:577392110
2102 2102 a, t dbSNP:768777565
2111 2111 -, g dbSNP:35957800
2158 2158 a, g dbSNP:749428064
2169 2169 c, t dbSNP:932972
2183 2183 g, t dbSNP:770894965

Target ORF information:

RefSeq Version XM_005245266
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005245266

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGAAGGGC TGAAGTCTAG AGGTCGCGGC TGGAGGCGTC AGTGCCGGAA GCGAGCCAGG 
CTGGGAACGT CCCCGCTGTG GCTTTGGCTG CCTGTCTTAC TCAAGTTTTC ATTATCTTCC
TGGGCAGCTG ATTGGCACCC CCAGGCCCAC ACTGAAAGCA TGTCGATCCA GGAGAACATA
TCATCCCTGC AGCTTCGGTC ATGGGTCTCT AAGTCCCAAA GAGACTTAGC AAAGTCCATC
CTGATTGGGG CTCCAGGAGG GCCAGCGGGG TATCTGCGGC GGGCCAGTGT GGCCCAACTG
ACCCAGGAGC TGGGCACTGC CTTCTTCCAG CAGCAGCAGC TGCCAGCTGC TATGGCAGAC
ACCTTCCTGG AACACCTCTG CCTACTGGAC ATTGACTCCG AGCCCGTGGC TGCTCGCAGT
ACCAGCATCA TTGCCACCAT CGGGCCAGCA TCTCGCTCCG TGGAGCGCCT CAAGGAGATG
ATCAAGGCCG GGATGAACAT TGCGCGACTC AACTTCTCCC ACGGCTCCCA CGAGTACCAT
GCTGAGTCCA TCGCCAACGT CCGGGAGGCG GTGGAGAGCT TTGCAGGTTC CCCACTCAGC
TACCGGCCCG TGGCCATCGC CCTGGACACC AAGGGACCGG AGATCCGCAC TGGGATCCTG
CAGGGGGGTC CAGAGTCGGA AGTGGAGCTG GTGAAGGGCT CCCAGGTGCT GGTGACTGTG
GACCCCGCGT TCCGGACGCG GGGGAACGCG AACACCGTGT GGGTGGACTA CCCCAATATT
GTCCGGGTCG TGCCGGTGGG GGGCCGCATC TACATTGACG ACGGGCTCAT CTCCCTAGTG
GTCCAGAAAA TCGGCCCAGA GGGACTGGTG ACCCAAGTGG AGAACGGCGG CGTCCTGGGC
AGCCGGAAGG GCGTGAACTT GCCAGGGGCC CAGGTGGACT TGCCCGGGCT GTCCGAGCAG
GACGTCCGAG ACCTGCGCTT CGGGGTGGAG CATGGGGTGG ACATCGTCTT TGCCTCCTTT
GTGCGGAAAG CCAGCGACGT GGCTGCCGTC AGGGCTGCTC TGGGTCCGGA AGGACACGGC
ATCAAGATCA TCAGCAAAAT TGAGAACCAC GAAGGCGTGA AGAGGTTTGA TGAAATCCTG
GAGGTGAGCG ACGGCATCAT GGTGGCACGG GGGGACCTAG GCATCGAGAT CCCAGCAGAG
AAGGTTTTCC TGGCTCAGAA GATGATGATT GGGCGCTGCA ACTTGGCGGG CAAGCCTGTT
GTCTGTGCCA CACAGATGCT GGAGAGCATG ATTACCAAGC CCCGGCCAAC GAGGGCAGAG
ACAAGCGATG TCGCCAATGC TGTGCTGGAT GGGGCTGACT GCATCATGCT GTCAGGGGAG
ACTGCCAAGG GCAACTTCCC TGTGGAAGCG GTGAAGATGC AGCATGCGAT TGCCCGGGAG
GCAGAGGCCG CAGTGTACCA CCGGCAGCTG TTTGAGGAGC TACGTCGGGC AGCGCCACTA
AGCCGTGATC CCACTGAGGT CACCGCCATT GGTGCTGTGG AGGCTGCCTT CAAGTGCTGT
GCTGCTGCCA TCATTGTGCT GACCACAACT GGCCGCTCAG CCCAGCTTCT GTCTCGGTAC
CGACCTCGGG CAGCAGTCAT TGCTGTCACC CGCTCTGCCC AGGCTGCCCG CCAGGTCCAC
TTATGCCGAG GAGTCTTCCC CTTGCTTTAC CGTGAACCTC CAGAAGCCAT CTGGGCAGAT
GATGTAGATC GCCGGGTGCA ATTTGGCATT GAAAGTGGAA AGCTCCGTGG CTTCCTCCGT
GTTGGAGACC TGGTGATTGT GGTGACAGGC TGGCGACCTG GCTCCGGCTA CACCAACATC
ATGCGGGTGC TAAGCATATC CTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu34476D
Sequence Information ORF Nucleotide Sequence (Length: 1533bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pyruvate kinase PKLR isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578801113. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCAGACA CCTTCCTGGA ACACCTCTGC CTACTGGACA TTGACTCCGA GCCCGTGGCT 
GCTCGCAGTA CCAGCATCAT TGCCACCATC GGGCCAGCAT CTCGCTCCGT GGAGCGCCTC
AAGGAGATGA TCAAGGCCGG GATGAACATT GCGCGACTCA ACTTCTCCCA CGGCTCCCAC
GAGTACCATG CTGAGTCCAT CGCCAACGTC CGGGAGGCGG TGGAGAGCTT TGCAGGTTCC
CCACTCAGCT ACCGGCCCGT GGCCATCGCC CTGGACACCA AGGGACCGGA GATCCGCACT
GGGATCCTGC AGGGGGGTCC AGAGTCGGAA GTGGAGCTGG TGAAGGGCTC CCAGGTGCTG
GTGACTGTGG ACCCCGCGTT CCGGACGCGG GGGAACGCGA ACACCGTGTG GGTGGACTAC
CCCAATATTG TCCGGGTCGT GCCGGTGGGG GGCCGCATCT ACATTGACGA CGGGCTCATC
TCCCTAGTGG TCCAGAAAAT CGGCCCAGAG GGACTGGTGA CCCAAGTGGA GAACGGCGGC
GTCCTGGGCA GCCGGAAGGG CGTGAACTTG CCAGGGGCCC AGGTGGACTT GCCCGGGCTG
TCCGAGCAGG ACGTCCGAGA CCTGCGCTTC GGGGTGGAGC ATGGGGTGGA CATCGTCTTT
GCCTCCTTTG TGCGGAAAGC CAGCGACGTG GCTGCCGTCA GGGCTGCTCT GGGTCCGGAA
GGACACGGCA TCAAGATCAT CAGCAAAATT GAGAACCACG AAGGCGTGAA GAGGTTTGAT
GAAATCCTGG AGGTGAGCGA CGGCATCATG GTGGCACGGG GGGACCTAGG CATCGAGATC
CCAGCAGAGA AGGTTTTCCT GGCTCAGAAG ATGATGATTG GGCGCTGCAA CTTGGCGGGC
AAGCCTGTTG TCTGTGCCAC ACAGATGCTG GAGAGCATGA TTACCAAGCC CCGGCCAACG
AGGGCAGAGA CAAGCGATGT CGCCAATGCT GTGCTGGATG GGGCTGACTG CATCATGCTG
TCAGGGGAGA CTGCCAAGGG CAACTTCCCT GTGGAAGCGG TGAAGATGCA GCATGCGATT
GCCCGGGAGG CAGAGGCCGC AGTGTACCAC CGGCAGCTGT TTGAGGAGCT ACGTCGGGCA
GCGCCACTAA GCCGTGATCC CACTGAGGTC ACCGCCATTG GTGCTGTGGA GGCTGCCTTC
AAGTGCTGTG CTGCTGCCAT CATTGTGCTG ACCACAACTG GCCGCTCAGC CCAGCTTCTG
TCTCGGTACC GACCTCGGGC AGCAGTCATT GCTGTCACCC GCTCTGCCCA GGCTGCCCGC
CAGGTCCACT TATGCCGAGG AGTCTTCCCC TTGCTTTACC GTGAACCTCC AGAAGCCATC
TGGGCAGATG ATGTAGATCG CCGGGTGCAA TTTGGCATTG AAAGTGGAAA GCTCCGTGGC
TTCCTCCGTG TTGGAGACCT GGTGATTGTG GTGACAGGCT GGCGACCTGG CTCCGGCTAC
ACCAACATCA TGCGGGTGCT AAGCATATCC TGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_006711449.1
CDS122..1654
Misc Feature(1)182..1648(+)
Misc Feature(2)185..1639(+)
Misc Feature(3)188..1462(+)
Misc Feature(4)275..1042(+)
Translation
Position Chain Variation Link
1 1 a, t dbSNP:747479690
5 5 c, t dbSNP:780460339
14 14 a, g dbSNP:375257968
16 16 c, t dbSNP:746119630
22 22 c, g dbSNP:778939180
23 23 c, t dbSNP:757391113
24 24 c, t dbSNP:753922649
26 26 a, t dbSNP:777978713
30 30 c, t dbSNP:762350074
45 45 a, g dbSNP:372152236
46 46 c, t dbSNP:766209695
49 49 g, t dbSNP:758061395
58 58 c, t dbSNP:137996782
59 59 c, t dbSNP:368867132
63 63 c, g dbSNP:761502347
65 65 c, g dbSNP:776156268
69 69 c, g dbSNP:373541741
72 72 a, g dbSNP:763586752
82 82 a, g dbSNP:761224118
88 88 a, g dbSNP:775921590
91 91 g, t dbSNP:772493914
92 92 g, t dbSNP:746180822
110 110 c, t dbSNP:8177962
122 122 a, g dbSNP:773589949
136 136 c, t dbSNP:770187539
141 141 a, t dbSNP:754696137
145 145 c, t dbSNP:762094018
146 146 c, t dbSNP:200791251
147 147 a, t dbSNP:140571200
152 152 c, t dbSNP:768765063
163 163 c, t dbSNP:745802541
167 167 a, t dbSNP:766051969
169 169 c, t dbSNP:185441545
170 170 a, g dbSNP:139707114
173 173 c, g dbSNP:151005140
175 175 c, t dbSNP:777525158
176 176 a, g dbSNP:368492204
177 177 c, t dbSNP:754785851
185 185 c, g dbSNP:752391774
186 186 a, g dbSNP:375471342
188 188 a, t dbSNP:754454017
201 201 c, t dbSNP:752037743
211 211 c, t dbSNP:147391950
212 212 a, g, t dbSNP:750857114
214 214 a, g dbSNP:759358280
217 217 a, g dbSNP:762937077
224 224 c, t dbSNP:148636159
225 225 a, g dbSNP:534895384
227 227 a, t dbSNP:762661462
229 229 c, g dbSNP:776268137
230 230 g, t dbSNP:143984223
236 236 a, c, t dbSNP:779765565
237 237 a, g dbSNP:371471086
244 244 a, g dbSNP:757921751
263 263 a, g dbSNP:746377052
270 270 c, t dbSNP:779517988
276 276 a, g, t dbSNP:754441130
279 279 g, t dbSNP:754282523
283 283 c, t dbSNP:764337069
297 297 c, g dbSNP:756428691
302 302 c, g dbSNP:752905261
303 303 a, c dbSNP:767598564
314 314 a, g dbSNP:773981429
318 318 a, c dbSNP:118204089
327 327 a, t dbSNP:770337533
328 328 a, c dbSNP:749817706
330 330 a, t dbSNP:574051756
332 332 a, c dbSNP:770175821
344 344 a, g dbSNP:748560715
345 345 a, t dbSNP:757997111
349 349 a, c dbSNP:781504670
361 361 c, t dbSNP:755258394
375 375 a, g dbSNP:757378318
384 384 c, g dbSNP:751716279
389 389 a, g dbSNP:780192373
392 392 c, t dbSNP:758341301
398 398 a, c dbSNP:753840260
403 403 a, g dbSNP:763884887
409 409 a, g dbSNP:146187567
416 416 c, t dbSNP:118204083
427 427 c, t dbSNP:771412921
428 428 c, t dbSNP:767419098
429 429 c, t dbSNP:759323460
434 434 c, g, t dbSNP:199824528
435 435 a, g dbSNP:762330662
436 436 a, g dbSNP:201674983
437 437 a, g dbSNP:779196057
443 443 c, g dbSNP:757359024
452 452 a, g dbSNP:370513342
453 453 c, t dbSNP:781236222
458 458 c, t dbSNP:138482544
461 461 a, g dbSNP:751361992
489 489 c, t dbSNP:370932346
491 491 g, t dbSNP:758042780
494 494 c, g dbSNP:577247981
508 508 a, g dbSNP:764731242
510 510 g, t dbSNP:552916457
514 514 g, t dbSNP:754361540
515 515 a, g dbSNP:781165565
517 517 c, g dbSNP:764631858
519 519 c, t dbSNP:761210207
523 523 a, c dbSNP:775787566
531 531 a, g dbSNP:772473652
544 544 -, c dbSNP:749541053
546 546 a, g dbSNP:759694655
547 547 c, t dbSNP:749833572
549 549 g, t dbSNP:780259014
555 555 a, g dbSNP:200480300
556 556 c, g dbSNP:749332866
560 560 c, g dbSNP:777980472
563 563 c, t dbSNP:768541145
564 564 c, t dbSNP:777667459
565 565 g, t dbSNP:779725913
567 567 c, t dbSNP:200979497
568 568 g, t dbSNP:750102822
574 574 a, c dbSNP:778484808
578 578 a, c dbSNP:756878847
584 584 a, t dbSNP:753384013
585 585 c, t dbSNP:200572803
586 586 c, t dbSNP:756559416
592 592 c, t dbSNP:761145518
595 595 a, g dbSNP:555325288
600 600 a, t dbSNP:768657605
603 603 c, t dbSNP:767781894
604 604 c, t dbSNP:759941126
609 609 a, t dbSNP:774736844
611 611 a, g dbSNP:771329959
615 615 a, g dbSNP:763237413
620 620 a, g dbSNP:764375399
623 623 g, t dbSNP:750606318
628 628 a, g dbSNP:765262843
636 636 c, t dbSNP:769475930
650 650 g, t dbSNP:201953584
651 651 a, t dbSNP:776546824
655 655 c, t dbSNP:772083920
656 656 a, g dbSNP:745797890
659 659 a, g dbSNP:759517847
660 660 a, g dbSNP:774192277
662 662 a, g dbSNP:555712170
674 674 c, t dbSNP:748947397
675 675 a, g dbSNP:368830326
676 676 a, g dbSNP:755602921
680 680 a, g dbSNP:267598065
685 685 c, g dbSNP:747744073
688 688 c, t dbSNP:780539049
694 694 a, g dbSNP:755610815
698 698 a, g dbSNP:752053420
699 699 c, t dbSNP:766853199
704 704 a, g dbSNP:750161685
707 707 a, c, g dbSNP:140575553
709 709 a, c dbSNP:765541284
712 712 g, t dbSNP:761905811
715 715 c, t dbSNP:148319141
719 719 c, t dbSNP:199959447
723 723 c, g dbSNP:769783555
728 728 a, c dbSNP:764143249
733 733 c, t dbSNP:576755609
734 734 g, t dbSNP:200695047
735 735 c, t dbSNP:770747923
737 737 a, c, g dbSNP:772860949
743 743 c, g dbSNP:147659527
746 746 c, t dbSNP:747753310
747 747 a, g dbSNP:141393570
752 752 a, c, g dbSNP:747549978
755 755 a, g dbSNP:780659178
755 755 -, g dbSNP:754939638
758 758 a, g dbSNP:147689373
763 763 c, t dbSNP:750836008
765 765 a, g, t dbSNP:758560647
766 766 c, g dbSNP:757554998
775 775 -, c dbSNP:751449001
787 787 a, c dbSNP:150532071
795 795 a, g dbSNP:749666187
798 798 a, g dbSNP:780875123
803 803 a, t dbSNP:749573131
809 809 g, t dbSNP:760709075
812 812 a, g dbSNP:751524706
813 813 c, t dbSNP:766353400
818 818 a, c, g dbSNP:148435198
825 825 a, c dbSNP:769464933
832 832 a, g dbSNP:761529212
835 835 c, t dbSNP:776382179
836 836 c, g dbSNP:201979697
837 837 c, t dbSNP:746566827
838 838 c, g dbSNP:200840994
847 847 a, c, t dbSNP:141571402
848 848 a, c, g dbSNP:373990660
849 849 g, t dbSNP:754124936
866 866 a, c dbSNP:777926506
868 868 a, g dbSNP:756311123
880 880 a, c, t dbSNP:745663677
882 882 a, g dbSNP:767580478
885 885 a, g dbSNP:763006858
887 887 c, g dbSNP:549295725
895 895 g, t dbSNP:763964110
900 900 a, g dbSNP:760413736
901 901 c, t dbSNP:8177974
908 908 c, g dbSNP:767088632
909 909 a, t dbSNP:758924903
920 920 a, g dbSNP:773893686
922 922 a, c, t dbSNP:138476691
923 923 a, g dbSNP:773626254
931 931 a, g dbSNP:756339496
938 938 c, g, t dbSNP:748376799
940 940 c, g dbSNP:781220573
944 944 a, g, t dbSNP:747097960
949 949 a, g dbSNP:780173822
952 952 c, g dbSNP:757318290
955 955 c, t dbSNP:376694187
956 956 a, g dbSNP:781715321
971 971 -, aag dbSNP:762301937
973 973 a, g dbSNP:763982504
978 978 a, t dbSNP:755934816
996 996 c, t dbSNP:752423472
999 999 c, t dbSNP:779152555
1004 1004 c, t dbSNP:138871700
1008 1008 c, g dbSNP:773803500
1010 1010 a, g dbSNP:765903674
1017 1017 a, c dbSNP:763466930
1018 1018 a, g dbSNP:773644908
1025 1025 c, g, t dbSNP:533316562
1028 1028 g, t dbSNP:777020292
1033 1033 -, ctgt dbSNP:749942178
1051 1051 a, g dbSNP:754739652
1057 1057 c, t dbSNP:751204824
1066 1066 c, t dbSNP:779728345
1071 1071 c, t dbSNP:758011557
1073 1073 a, c, t dbSNP:140964046
1075 1075 a, g dbSNP:764709204
1076 1076 c, g, t dbSNP:767333188
1078 1078 a, t dbSNP:764396178
1080 1080 c, t dbSNP:74315362
1081 1081 a, g dbSNP:761048881
1087 1087 a, c dbSNP:775697482
1088 1088 a, g dbSNP:147089210
1095 1095 a, g dbSNP:548802413
1096 1096 c, t dbSNP:772465237
1097 1097 a, g dbSNP:147034239
1098 1098 a, g dbSNP:759834904
1099 1099 c, t dbSNP:141596564
1101 1101 c, t dbSNP:771080781
1102 1102 c, t dbSNP:748044200
1107 1107 a, g dbSNP:776594413
1115 1115 c, t dbSNP:761407486
1131 1131 a, g dbSNP:766913459
1137 1137 c, t dbSNP:768361068
1138 1138 a, g dbSNP:368784290
1145 1145 a, g dbSNP:375456388
1146 1146 a, g dbSNP:779817999
1149 1149 a, t dbSNP:780985369
1161 1161 a, c, g dbSNP:745461639
1164 1164 a, c dbSNP:774006353
1165 1165 c, t dbSNP:756750467
1168 1168 c, t dbSNP:754321902
1175 1175 a, g dbSNP:764713394
1179 1179 c, t dbSNP:756549612
1180 1180 a, g, t dbSNP:767981692
1181 1181 a, g dbSNP:759855502
1190 1190 a, c dbSNP:118204084
1197 1197 a, c dbSNP:763528176
1198 1198 a, g dbSNP:774652817
1199 1199 -, a dbSNP:751363828
1202 1202 a, g dbSNP:756703156
1203 1203 a, c dbSNP:753198136
1206 1206 a, g dbSNP:768002493
1219 1219 c, t dbSNP:755357782
1220 1220 a, g dbSNP:762591322
1232 1232 c, t dbSNP:142238268
1235 1235 c, t dbSNP:778805401
1236 1236 a, g dbSNP:758965704
1247 1247 a, g, t dbSNP:771145576
1253 1253 c, t dbSNP:773396824
1254 1254 a, g dbSNP:370554398
1257 1257 a, g dbSNP:760606711
1263 1263 c, t dbSNP:531294009
1264 1264 a, g dbSNP:771876200
1268 1268 a, c, t dbSNP:774102445
1274 1274 c, t dbSNP:189360283
1275 1275 a, g dbSNP:149932430
1282 1282 c, t dbSNP:777357253
1287 1287 a, c dbSNP:769123407
1292 1292 a, g dbSNP:748700869
1294 1294 c, t dbSNP:146708702
1295 1295 a, g dbSNP:756864028
1298 1298 a, g dbSNP:377022809
1302 1302 a, g dbSNP:755522396
1307 1307 a, g dbSNP:752034960
1308 1308 c, t dbSNP:149946271
1312 1312 a, g dbSNP:758655787
1332 1332 c, g dbSNP:750540943
1337 1337 g, t dbSNP:765388322
1342 1342 a, c dbSNP:761831271
1349 1349 c, t dbSNP:753890733
1351 1351 c, g dbSNP:572233729
1358 1358 a, g dbSNP:759466273
1363 1363 a, c dbSNP:774262110
1364 1364 c, t dbSNP:201406712
1365 1365 a, g dbSNP:118204085
1371 1371 c, t dbSNP:773040738
1375 1375 a, g dbSNP:765024950
1378 1378 g, t dbSNP:761542089
1385 1385 c, t dbSNP:116100695
1386 1386 a, g dbSNP:768210809
1392 1392 a, g dbSNP:369183199
1397 1397 c, t dbSNP:200133000
1398 1398 a, g, t dbSNP:746257586
1405 1405 a, g, t dbSNP:757534925
1407 1407 a, t dbSNP:749456521
1413 1413 c, t dbSNP:141560532
1420 1420 c, t dbSNP:756147719
1421 1421 a, c, t dbSNP:551883218
1422 1422 a, g dbSNP:758327704
1437 1437 c, t dbSNP:750255401
1440 1440 g, t dbSNP:185753709
1441 1441 a, c dbSNP:761558916
1445 1445 a, g dbSNP:8177988
1447 1447 c, t dbSNP:376065116
1448 1448 a, c dbSNP:760152160
1450 1450 c, t dbSNP:201011399
1454 1454 c, t dbSNP:760208078
1458 1458 a, g dbSNP:113403872
1468 1468 c, t dbSNP:371489505
1476 1476 c, t dbSNP:779681839
1481 1481 c, t dbSNP:139002629
1482 1482 a, g dbSNP:771218326
1483 1483 a, t dbSNP:749544468
1486 1486 a, c dbSNP:761819694
1487 1487 a, c, t dbSNP:562318137
1490 1490 c, t dbSNP:756341032
1508 1508 a, g dbSNP:748219831
1523 1523 c, t dbSNP:201255024
1524 1524 a, g dbSNP:758278200
1525 1525 c, g dbSNP:750305814
1528 1528 g, t dbSNP:192697407
1533 1533 c, t dbSNP:754526647
1537 1537 c, t dbSNP:757065547
1543 1543 a, t dbSNP:201217064
1547 1547 g, t dbSNP:763878359
1557 1557 a, g dbSNP:752328700
1558 1558 c, t dbSNP:766970429
1560 1560 g, t dbSNP:759039445
1568 1568 c, t dbSNP:751050425
1569 1569 a, g dbSNP:375525211
1574 1574 a, g dbSNP:190966689
1583 1583 a, g dbSNP:370316462
1584 1584 c, t dbSNP:773628430
1592 1592 g, t dbSNP:770125793
1602 1602 a, g dbSNP:370938255
1604 1604 c, t dbSNP:532230312
1605 1605 -, gacctggc dbSNP:771142025
1615 1615 a, c, t dbSNP:140859641
1628 1628 a, t dbSNP:780275059
1630 1630 c, t dbSNP:771049942
1632 1632 c, t dbSNP:749179500
1633 1633 a, g dbSNP:777435169
1634 1634 a, c, t dbSNP:1052176
1635 1635 a, g dbSNP:61755431
1639 1639 c, g dbSNP:552203379
1642 1642 a, g dbSNP:761092859
1657 1657 a, c, t dbSNP:368150636
1658 1658 a, g dbSNP:765864419
1666 1666 a, c dbSNP:758922618
1667 1667 c, g, t dbSNP:1052177
1668 1668 c, g, t dbSNP:8177994
1669 1669 c, g, t dbSNP:374751259
1674 1674 a, g dbSNP:775724792
1676 1676 a, c dbSNP:772210964
1688 1688 c, t dbSNP:749269783
1692 1692 c, t dbSNP:773153185
1711 1711 a, g dbSNP:569710209
1716 1716 c, t dbSNP:542249964
1726 1726 a, c dbSNP:530076782
1732 1732 c, t dbSNP:563189341
1735 1735 c, t dbSNP:186058494
1739 1739 a, g dbSNP:768976043
1790 1790 a, g dbSNP:762039790
1817 1817 c, t dbSNP:774442850
1819 1819 a, g dbSNP:772906215
1846 1846 a, t dbSNP:577392110
1854 1854 a, t dbSNP:768777565
1863 1863 -, g dbSNP:35957800
1910 1910 a, g dbSNP:749428064
1921 1921 c, t dbSNP:932972
1935 1935 g, t dbSNP:770894965

Target ORF information:

RefSeq Version XM_006711386
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_006711386

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCAGACA CCTTCCTGGA ACACCTCTGC CTACTGGACA TTGACTCCGA GCCCGTGGCT 
GCTCGCAGTA CCAGCATCAT TGCCACCATC GGGCCAGCAT CTCGCTCCGT GGAGCGCCTC
AAGGAGATGA TCAAGGCCGG GATGAACATT GCGCGACTCA ACTTCTCCCA CGGCTCCCAC
GAGTACCATG CTGAGTCCAT CGCCAACGTC CGGGAGGCGG TGGAGAGCTT TGCAGGTTCC
CCACTCAGCT ACCGGCCCGT GGCCATCGCC CTGGACACCA AGGGACCGGA GATCCGCACT
GGGATCCTGC AGGGGGGTCC AGAGTCGGAA GTGGAGCTGG TGAAGGGCTC CCAGGTGCTG
GTGACTGTGG ACCCCGCGTT CCGGACGCGG GGGAACGCGA ACACCGTGTG GGTGGACTAC
CCCAATATTG TCCGGGTCGT GCCGGTGGGG GGCCGCATCT ACATTGACGA CGGGCTCATC
TCCCTAGTGG TCCAGAAAAT CGGCCCAGAG GGACTGGTGA CCCAAGTGGA GAACGGCGGC
GTCCTGGGCA GCCGGAAGGG CGTGAACTTG CCAGGGGCCC AGGTGGACTT GCCCGGGCTG
TCCGAGCAGG ACGTCCGAGA CCTGCGCTTC GGGGTGGAGC ATGGGGTGGA CATCGTCTTT
GCCTCCTTTG TGCGGAAAGC CAGCGACGTG GCTGCCGTCA GGGCTGCTCT GGGTCCGGAA
GGACACGGCA TCAAGATCAT CAGCAAAATT GAGAACCACG AAGGCGTGAA GAGGTTTGAT
GAAATCCTGG AGGTGAGCGA CGGCATCATG GTGGCACGGG GGGACCTAGG CATCGAGATC
CCAGCAGAGA AGGTTTTCCT GGCTCAGAAG ATGATGATTG GGCGCTGCAA CTTGGCGGGC
AAGCCTGTTG TCTGTGCCAC ACAGATGCTG GAGAGCATGA TTACCAAGCC CCGGCCAACG
AGGGCAGAGA CAAGCGATGT CGCCAATGCT GTGCTGGATG GGGCTGACTG CATCATGCTG
TCAGGGGAGA CTGCCAAGGG CAACTTCCCT GTGGAAGCGG TGAAGATGCA GCATGCGATT
GCCCGGGAGG CAGAGGCCGC AGTGTACCAC CGGCAGCTGT TTGAGGAGCT ACGTCGGGCA
GCGCCACTAA GCCGTGATCC CACTGAGGTC ACCGCCATTG GTGCTGTGGA GGCTGCCTTC
AAGTGCTGTG CTGCTGCCAT CATTGTGCTG ACCACAACTG GCCGCTCAGC CCAGCTTCTG
TCTCGGTACC GACCTCGGGC AGCAGTCATT GCTGTCACCC GCTCTGCCCA GGCTGCCCGC
CAGGTCCACT TATGCCGAGG AGTCTTCCCC TTGCTTTACC GTGAACCTCC AGAAGCCATC
TGGGCAGATG ATGTAGATCG CCGGGTGCAA TTTGGCATTG AAAGTGGAAA GCTCCGTGGC
TTCCTCCGTG TTGGAGACCT GGTGATTGTG GTGACAGGCT GGCGACCTGG CTCCGGCTAC
ACCAACATCA TGCGGGTGCT AAGCATATCC TGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu59731D
Sequence Information ORF Nucleotide Sequence (Length: 1311bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pyruvate kinase PKLR isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGAAGGGC TGAAGTCTAG AGGTCGCGGC TGGAGGCGTC AGTGCCGGAA GCGAGCCAGG 
CTGGGAACGT CCCCGCTGTG GCTTTGGCTG CCTGTCTTAC TCAAGTTTTC ATTATCTTCC
TGGGCAGCTG ATTGGCACCC CCAGGCCCAC ACTGAAAGCA TGTCGATCCA GGAGAACATA
TCATCCCTGC AGCTTCGGTC ATGGGTCTCT AAGTCCCAAA GAGACTTAGC AAAGTCCATC
CTGATTGGGG CTCCAGGAGG GCCAGCGGGG TATCTGCGGC GGGCCAGTGT GGCCCAACTG
ACCCAGGAGC TGGGCACTGC CTTCTTCCAG CAGCAGCAGC TGCCAGCTGC TATGGCAGAC
ACCTTCCTGG AACACCTCTG CCTACTGGAC ATTGACTCCG AGCCCGTGGC TGCTCGCAGT
ACCAGCATCA TTGCCACCAT CGGGCCAGCA TCTCGCTCCG TGGAGCGCCT CAAGGAGATG
ATCAAGGCCG GGATGAACAT TGCGCGACTC AACTTCTCCC ACGGCTCCCA CGAGTACCAT
GCTGAGTCCA TCGCCAACGT CCGGGAGGCG GTGGAGAGCT TTGCAGGTTC CCCACTCAGC
TACCGGCCCG TGGCCATCGC CCTGGACACC AAGGGACCGG AGATCCGCAC TGGGATCCTG
CAGGGGGGTC CAGAGTCGGA AGTGGAGCTG GTGAAGGGCT CCCAGGTGCT GGTGACTGTG
GACCCCGCGT TCCGGACGCG GGGGAACGCG AACACCGTGT GGGTGGACTA CCCCAATATT
GTCCGGGTCG TGCCGGTGGG GGGCCGCATC TACATTGACG ACGGGCTCAT CTCCCTAGTG
GTCCAGAAAA TCGGCCCAGA GGGACTGGTG ACCCAAGTGG AGAACGGCGG CGTCCTGGGC
AGCCGGAAGG GCGTGAACTT GCCAGGGGCC CAGGTGGACT TGCCCGGGCT GTCCGAGCAG
GACGTCCGAG ACCTGCGCTT CGGGGTGGAG CATGGGGTGG ACATCGTCTT TGCCTCCTTT
GTGCGGAAAG CCAGCGACGT GGCTGCCGTC AGGGCTGCTC TGGGTCCGGA AGGACACGGC
ATCAAGATCA TCAGCAAAAT TGAGAACCAC GAAGGCGTGA AGAGGTTTGA TGAAATCCTG
GAGGTGAGCG ACGGCATCAT GGTGGCACGG GGGGACCTAG GCATCGAGAT CCCAGCAGAG
AAGGTTTTCC TGGCTCAGAA GATGATGATT GGGCGCTGCA ACTTGGCGGG CAAGCCTGTT
GTCTGTGCCA CACAGGTCTG GAGTGAGGCC TTGAGATGCT GGAGAGCATTG A
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011507941.1
CDS19..1329
Misc Feature(1)430..>1293(+)
Misc Feature(2)436..1275(+)
Misc Feature(3)523..1290(+)
Translation
Position Chain Variation Link
60 60 a, g dbSNP:12043597
64 64 a, c dbSNP:754038530
65 65 a, g dbSNP:761345776
93 93 a, g dbSNP:573445586
150 150 c, t dbSNP:766696826
157 157 c, t dbSNP:751786807
159 159 c, t dbSNP:766550230
160 160 c, g dbSNP:758498340
165 165 c, t dbSNP:750568645
169 169 a, g dbSNP:765203859
171 171 c, t dbSNP:760728696
176 176 a, c, g, t dbSNP:201169481
182 182 a, c dbSNP:774176341
183 183 a, g dbSNP:139697646
189 189 g, t dbSNP:758112712
197 197 c, t dbSNP:762490269
214 214 c, t dbSNP:772818969
215 215 a, g dbSNP:769174570
220 220 c, t dbSNP:748703432
222 222 a, g dbSNP:750812851
229 229 a, g dbSNP:781610938
233 233 c, g dbSNP:769038526
236 236 a, g dbSNP:200357340
247 247 a, g dbSNP:754783713
254 254 c, g dbSNP:747323509
260 260 c, t dbSNP:780370877
263 263 c, t dbSNP:142735355
266 266 a, g dbSNP:771523060
269 269 c, t dbSNP:150077703
284 284 c, g, t dbSNP:770019694
285 285 a, g dbSNP:148376707
287 287 a, g, t dbSNP:118204087
288 288 -, g dbSNP:762615239
288 288 a, g, t dbSNP:141341788
292 292 c, t dbSNP:758304847
296 296 g, t dbSNP:745811076
298 298 c, t dbSNP:375189218
299 299 a, g, t dbSNP:374805791
306 306 c, t dbSNP:777368485
308 308 a, t dbSNP:753415917
315 315 a, g dbSNP:370696464
325 325 a, g dbSNP:752199380
327 327 a, g dbSNP:767023470
331 331 g, t dbSNP:760029571
332 332 a, g dbSNP:752093404
334 334 a, t dbSNP:760443294
337 337 a, g dbSNP:753089348
344 344 a, t dbSNP:746308116
346 346 c, t dbSNP:772567442
348 348 a, g dbSNP:200581879
358 358 c, t dbSNP:8177962
370 370 a, g dbSNP:773589949
384 384 c, t dbSNP:770187539
389 389 a, t dbSNP:754696137
393 393 c, t dbSNP:762094018
394 394 c, t dbSNP:200791251
395 395 a, t dbSNP:140571200
400 400 c, t dbSNP:768765063
411 411 c, t dbSNP:745802541
415 415 a, t dbSNP:766051969
417 417 c, t dbSNP:185441545
418 418 a, g dbSNP:139707114
421 421 c, g dbSNP:151005140
423 423 c, t dbSNP:777525158
424 424 a, g dbSNP:368492204
425 425 c, t dbSNP:754785851
433 433 c, g dbSNP:752391774
434 434 a, g dbSNP:375471342
436 436 a, t dbSNP:754454017
449 449 c, t dbSNP:752037743
459 459 c, t dbSNP:147391950
460 460 a, g, t dbSNP:750857114
462 462 a, g dbSNP:759358280
465 465 a, g dbSNP:762937077
472 472 c, t dbSNP:148636159
473 473 a, g dbSNP:534895384
475 475 a, t dbSNP:762661462
477 477 c, g dbSNP:776268137
478 478 g, t dbSNP:143984223
484 484 a, c, t dbSNP:779765565
485 485 a, g dbSNP:371471086
492 492 a, g dbSNP:757921751
511 511 a, g dbSNP:746377052
518 518 c, t dbSNP:779517988
524 524 a, g, t dbSNP:754441130
527 527 g, t dbSNP:754282523
531 531 c, t dbSNP:764337069
545 545 c, g dbSNP:756428691
550 550 c, g dbSNP:752905261
551 551 a, c dbSNP:767598564
562 562 a, g dbSNP:773981429
566 566 a, c dbSNP:118204089
575 575 a, t dbSNP:770337533
576 576 a, c dbSNP:749817706
578 578 a, t dbSNP:574051756
580 580 a, c dbSNP:770175821
592 592 a, g dbSNP:748560715
593 593 a, t dbSNP:757997111
597 597 a, c dbSNP:781504670
609 609 c, t dbSNP:755258394
623 623 a, g dbSNP:757378318
632 632 c, g dbSNP:751716279
637 637 a, g dbSNP:780192373
640 640 c, t dbSNP:758341301
646 646 a, c dbSNP:753840260
651 651 a, g dbSNP:763884887
657 657 a, g dbSNP:146187567
664 664 c, t dbSNP:118204083
675 675 c, t dbSNP:771412921
676 676 c, t dbSNP:767419098
677 677 c, t dbSNP:759323460
682 682 c, g, t dbSNP:199824528
683 683 a, g dbSNP:762330662
684 684 a, g dbSNP:201674983
685 685 a, g dbSNP:779196057
691 691 c, g dbSNP:757359024
700 700 a, g dbSNP:370513342
701 701 c, t dbSNP:781236222
706 706 c, t dbSNP:138482544
709 709 a, g dbSNP:751361992
737 737 c, t dbSNP:370932346
739 739 g, t dbSNP:758042780
742 742 c, g dbSNP:577247981
756 756 a, g dbSNP:764731242
758 758 g, t dbSNP:552916457
762 762 g, t dbSNP:754361540
763 763 a, g dbSNP:781165565
765 765 c, g dbSNP:764631858
767 767 c, t dbSNP:761210207
771 771 a, c dbSNP:775787566
779 779 a, g dbSNP:772473652
792 792 -, c dbSNP:749541053
794 794 a, g dbSNP:759694655
795 795 c, t dbSNP:749833572
797 797 g, t dbSNP:780259014
803 803 a, g dbSNP:200480300
804 804 c, g dbSNP:749332866
808 808 c, g dbSNP:777980472
811 811 c, t dbSNP:768541145
812 812 c, t dbSNP:777667459
813 813 g, t dbSNP:779725913
815 815 c, t dbSNP:200979497
816 816 g, t dbSNP:750102822
822 822 a, c dbSNP:778484808
826 826 a, c dbSNP:756878847
832 832 a, t dbSNP:753384013
833 833