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PKLR cDNA ORF clone, Homo sapiens (human)

Gene Symbol PKLR
Entrez Gene ID 5313
Full Name pyruvate kinase, liver and RBC
Synonyms PK1, PKL, PKR, PKRL, RPK
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q21

Summary The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

609712

Disorder Html: Pyruvate kinase deficiency, 266200 (3); Adenosine triphosphate,

mRNA and Protein(s)

mRNA Protein Name
XM_006726212 XP_006726275 pyruvate kinase PKLR isoform X4
XM_011546931 XP_011545233 pyruvate kinase PKLR isoform X3
XM_011546932 XP_011545234 pyruvate kinase PKLR isoform X4
XM_005245266 XP_005245323 pyruvate kinase PKLR isoform X1
XM_006711386 XP_006711449 pyruvate kinase PKLR isoform X4
XM_011509639 XP_011507941 pyruvate kinase PKLR isoform X2
XM_011509640 XP_011507942 pyruvate kinase PKLR isoform X4
NM_000298 NP_000289 pyruvate kinase PKLR isoform 1
NM_181871 NP_870986 pyruvate kinase PKLR isoform 2



Homo sapiens (human) PKLR NP_000289.1
Pan troglodytes (chimpanzee) PKLR XP_524896.4
Macaca mulatta (Rhesus monkey) PKLR XP_001112902.2
Canis lupus familiaris (dog) PKLR NP_001243191.1
Bos taurus (cattle) PKLR NP_001069644.1
Mus musculus (house mouse) Pklr NP_038659.2
Rattus norvegicus (Norway rat) Pklr NP_036756.3
Danio rerio (zebrafish) pklr NP_958446.1
Arabidopsis thaliana (thale cress) AT3G04050 NP_187055.1
Arabidopsis thaliana (thale cress) AT3G55650 NP_191124.1
Arabidopsis thaliana (thale cress) AT3G25960 NP_189225.1
Arabidopsis thaliana (thale cress) AT3G55810 NP_191140.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PKLR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PKLR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu34476 XM_006726212 PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu59730 XM_011546931 PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu34476 XM_011546932 PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu34475 XM_005245266 PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu34476 XM_006711386 PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu59731 XM_011509639 PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu34476 XM_011509640 PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu26995
NM_000298 Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu27268 NM_181871 Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $321.30
$459.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu34476
Clone ID Related Accession (Same CDS sequence) XM_006711386 , XM_006726212 , XM_011509640 , XM_011546932
Accession Version XM_006726212.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1533bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pyruvate kinase PKLR isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003315906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578839333. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCAGACA CCTTCCTGGA ACACCTCTGC CTACTGGACA TTGACTCCGA GCCCGTGGCT 
GCTCGCAGTA CCAGCATCAT TGCCACCATC GGGCCAGCAT CTCGCTCCGT GGAGCGCCTC
AAGGAGATGA TCAAGGCCGG GATGAACATT GCGCGACTCA ACTTCTCCCA CGGCTCCCAC
GAGTACCATG CTGAGTCCAT CGCCAACGTC CGGGAGGCGG TGGAGAGCTT TGCAGGTTCC
CCACTCAGCT ACCGGCCCGT GGCCATCGCC CTGGACACCA AGGGACCGGA GATCCGCACT
GGGATCCTGC AGGGGGGTCC AGAGTCGGAA GTGGAGCTGG TGAAGGGCTC CCAGGTGCTG
GTGACTGTGG ACCCCGCGTT CCGGACGCGG GGGAACGCGA ACACCGTGTG GGTGGACTAC
CCCAATATTG TCCGGGTCGT GCCGGTGGGG GGCCGCATCT ACATTGACGA CGGGCTCATC
TCCCTAGTGG TCCAGAAAAT CGGCCCAGAG GGACTGGTGA CCCAAGTGGA GAACGGCGGC
GTCCTGGGCA GCCGGAAGGG CGTGAACTTG CCAGGGGCCC AGGTGGACTT GCCCGGGCTG
TCCGAGCAGG ACGTCCGAGA CCTGCGCTTC GGGGTGGAGC ATGGGGTGGA CATCGTCTTT
GCCTCCTTTG TGCGGAAAGC CAGCGACGTG GCTGCCGTCA GGGCTGCTCT GGGTCCGGAA
GGACACGGCA TCAAGATCAT CAGCAAAATT GAGAACCACG AAGGCGTGAA GAGGTTTGAT
GAAATCCTGG AGGTGAGCGA CGGCATCATG GTGGCACGGG GGGACCTAGG CATCGAGATC
CCAGCAGAGA AGGTTTTCCT GGCTCAGAAG ATGATGATTG GGCGCTGCAA CTTGGCGGGC
AAGCCTGTTG TCTGTGCCAC ACAGATGCTG GAGAGCATGA TTACCAAGCC CCGGCCAACG
AGGGCAGAGA CAAGCGATGT CGCCAATGCT GTGCTGGATG GGGCTGACTG CATCATGCTG
TCAGGGGAGA CTGCCAAGGG CAACTTCCCT GTGGAAGCGG TGAAGATGCA GCATGCGATT
GCCCGGGAGG CAGAGGCCGC AGTGTACCAC CGGCAGCTGT TTGAGGAGCT ACGTCGGGCA
GCGCCACTAA GCCGTGATCC CACTGAGGTC ACCGCCATTG GTGCTGTGGA GGCTGCCTTC
AAGTGCTGTG CTGCTGCCAT CATTGTGCTG ACCACAACTG GCCGCTCAGC CCAGCTTCTG
TCTCGGTACC GACCTCGGGC AGCAGTCATT GCTGTCACCC GCTCTGCCCA GGCTGCCCGC
CAGGTCCACT TATGCCGAGG AGTCTTCCCC TTGCTTTACC GTGAACCTCC AGAAGCCATC
TGGGCAGATG ATGTAGATCG CCGGGTGCAA TTTGGCATTG AAAGTGGAAA GCTCCGTGGC
TTCCTCCGTG TTGGAGACCT GGTGATTGTG GTGACAGGCT GGCGACCTGG CTCCGGCTAC
ACCAACATCA TGCGGGTGCT AAGCATATCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006726275.1
CDS126..1658
Misc Feature(1)186..1652(+)
Misc Feature(2)189..1643(+)
Misc Feature(3)192..1466(+)
Misc Feature(4)279..1046(+)
Translation

Target ORF information:

RefSeq Version XM_006726212
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006726212

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCAGACA CCTTCCTGGA ACACCTCTGC CTACTGGACA TTGACTCCGA GCCCGTGGCT 
GCTCGCAGTA CCAGCATCAT TGCCACCATC GGGCCAGCAT CTCGCTCCGT GGAGCGCCTC
AAGGAGATGA TCAAGGCCGG GATGAACATT GCGCGACTCA ACTTCTCCCA CGGCTCCCAC
GAGTACCATG CTGAGTCCAT CGCCAACGTC CGGGAGGCGG TGGAGAGCTT TGCAGGTTCC
CCACTCAGCT ACCGGCCCGT GGCCATCGCC CTGGACACCA AGGGACCGGA GATCCGCACT
GGGATCCTGC AGGGGGGTCC AGAGTCGGAA GTGGAGCTGG TGAAGGGCTC CCAGGTGCTG
GTGACTGTGG ACCCCGCGTT CCGGACGCGG GGGAACGCGA ACACCGTGTG GGTGGACTAC
CCCAATATTG TCCGGGTCGT GCCGGTGGGG GGCCGCATCT ACATTGACGA CGGGCTCATC
TCCCTAGTGG TCCAGAAAAT CGGCCCAGAG GGACTGGTGA CCCAAGTGGA GAACGGCGGC
GTCCTGGGCA GCCGGAAGGG CGTGAACTTG CCAGGGGCCC AGGTGGACTT GCCCGGGCTG
TCCGAGCAGG ACGTCCGAGA CCTGCGCTTC GGGGTGGAGC ATGGGGTGGA CATCGTCTTT
GCCTCCTTTG TGCGGAAAGC CAGCGACGTG GCTGCCGTCA GGGCTGCTCT GGGTCCGGAA
GGACACGGCA TCAAGATCAT CAGCAAAATT GAGAACCACG AAGGCGTGAA GAGGTTTGAT
GAAATCCTGG AGGTGAGCGA CGGCATCATG GTGGCACGGG GGGACCTAGG CATCGAGATC
CCAGCAGAGA AGGTTTTCCT GGCTCAGAAG ATGATGATTG GGCGCTGCAA CTTGGCGGGC
AAGCCTGTTG TCTGTGCCAC ACAGATGCTG GAGAGCATGA TTACCAAGCC CCGGCCAACG
AGGGCAGAGA CAAGCGATGT CGCCAATGCT GTGCTGGATG GGGCTGACTG CATCATGCTG
TCAGGGGAGA CTGCCAAGGG CAACTTCCCT GTGGAAGCGG TGAAGATGCA GCATGCGATT
GCCCGGGAGG CAGAGGCCGC AGTGTACCAC CGGCAGCTGT TTGAGGAGCT ACGTCGGGCA
GCGCCACTAA GCCGTGATCC CACTGAGGTC ACCGCCATTG GTGCTGTGGA GGCTGCCTTC
AAGTGCTGTG CTGCTGCCAT CATTGTGCTG ACCACAACTG GCCGCTCAGC CCAGCTTCTG
TCTCGGTACC GACCTCGGGC AGCAGTCATT GCTGTCACCC GCTCTGCCCA GGCTGCCCGC
CAGGTCCACT TATGCCGAGG AGTCTTCCCC TTGCTTTACC GTGAACCTCC AGAAGCCATC
TGGGCAGATG ATGTAGATCG CCGGGTGCAA TTTGGCATTG AAAGTGGAAA GCTCCGTGGC
TTCCTCCGTG TTGGAGACCT GGTGATTGTG GTGACAGGCT GGCGACCTGG CTCCGGCTAC
ACCAACATCA TGCGGGTGCT AAGCATATCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59730
Clone ID Related Accession (Same CDS sequence) XM_011546931
Accession Version XM_011546931.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1185bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pyruvate kinase PKLR isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003315906.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGTCCCGC AGCCCCAGGC CCACACTGAA AGCATGTCGA TCCAGGAGAA CATATCATCC 
CTGCAGCTTC GGTCATGGGT CTCTAAGTCC CAAAGAGACT TAGCAAAGTC CATCCTGATT
GGGGCTCCAG GAGGGCCAGC GGGGTATCTG CGGCGGGCCA GTGTGGCCCA ACTGACCCAG
GAGCTGGGCA CTGCCTTCTT CCAGCAGCAG CAGCTGCCAG CTGCTATGGC AGACACCTTC
CTGGAACACC TCTGCCTACT GGACATTGAC TCCGAGCCCG TGGCTGCTCG CAGTACCAGC
ATCATTGCCA CCATCGGGCC AGCATCTCGC TCCGTGGAGC GCCTCAAGGA GATGATCAAG
GCCGGGATGA ACATTGCGCG ACTCAACTTC TCCCACGGCT CCCACGAGTA CCATGCTGAG
TCCATCGCCA ACGTCCGGGA GGCGGTGGAG AGCTTTGCAG GTTCCCCACT CAGCTACCGG
CCCGTGGCCA TCGCCCTGGA CACCAAGGGA CCGGAGATCC GCACTGGGAT CCTGCAGGGG
GGTCCAGAGT CGGAAGTGGA GCTGGTGAAG GGCTCCCAGG TGCTGGTGAC TGTGGACCCC
GCGTTCCGGA CGCGGGGGAA CGCGAACACC GTGTGGGTGG ACTACCCCAA TATTGTCCGG
GTCGTGCCGG TGGGGGGCCG CATCTACATT GACGACGGGC TCATCTCCCT AGTGGTCCAG
AAAATCGGCC CAGAGGGACT GGTGACCCAA GTGGAGAACG GCGGCGTCCT GGGCAGCCGG
AAGGGCGTGA ACTTGCCAGG GGCCCAGGTG GACTTGCCCG GGCTGTCCGA GCAGGACGTC
CGAGACCTGC GCTTCGGGGT GGAGCATGGG GTGGACATCG TCTTTGCCTC CTTTGTGCGG
AAAGCCAGCG ACGTGGCTGC CGTCAGGGCT GCTCTGGGTC CGGAAGGACA CGGCATCAAG
ATCATCAGCA AAATTGAGAA CCACGAAGGC GTGAAGAGGT TTGATGAAAT CCTGGAGGTG
AGCGACGGCA TCATGGTGGC ACGGGGGGAC CTAGGCATCG AGATCCCAGC AGAGAAGGTT
TTCCTGGCTC AGAAGATGAT GATTGGGCGC TGCAACTTGG CGGGCAAGCC TGTTGTCTGT
GCCACACAGG TCTGGAGTGA GGCCTTGAGA TGCTGGAGAG CATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011545233.1
CDS12..1196
Misc Feature(1)297..>1160(+)
Misc Feature(2)303..1142(+)
Misc Feature(3)390..1157(+)
Translation

Target ORF information:

RefSeq Version XM_011546931
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011546931

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGTCCCGC AGCCCCAGGC CCACACTGAA AGCATGTCGA TCCAGGAGAA CATATCATCC 
CTGCAGCTTC GGTCATGGGT CTCTAAGTCC CAAAGAGACT TAGCAAAGTC CATCCTGATT
GGGGCTCCAG GAGGGCCAGC GGGGTATCTG CGGCGGGCCA GTGTGGCCCA ACTGACCCAG
GAGCTGGGCA CTGCCTTCTT CCAGCAGCAG CAGCTGCCAG CTGCTATGGC AGACACCTTC
CTGGAACACC TCTGCCTACT GGACATTGAC TCCGAGCCCG TGGCTGCTCG CAGTACCAGC
ATCATTGCCA CCATCGGGCC AGCATCTCGC TCCGTGGAGC GCCTCAAGGA GATGATCAAG
GCCGGGATGA ACATTGCGCG ACTCAACTTC TCCCACGGCT CCCACGAGTA CCATGCTGAG
TCCATCGCCA ACGTCCGGGA GGCGGTGGAG AGCTTTGCAG GTTCCCCACT CAGCTACCGG
CCCGTGGCCA TCGCCCTGGA CACCAAGGGA CCGGAGATCC GCACTGGGAT CCTGCAGGGG
GGTCCAGAGT CGGAAGTGGA GCTGGTGAAG GGCTCCCAGG TGCTGGTGAC TGTGGACCCC
GCGTTCCGGA CGCGGGGGAA CGCGAACACC GTGTGGGTGG ACTACCCCAA TATTGTCCGG
GTCGTGCCGG TGGGGGGCCG CATCTACATT GACGACGGGC TCATCTCCCT AGTGGTCCAG
AAAATCGGCC CAGAGGGACT GGTGACCCAA GTGGAGAACG GCGGCGTCCT GGGCAGCCGG
AAGGGCGTGA ACTTGCCAGG GGCCCAGGTG GACTTGCCCG GGCTGTCCGA GCAGGACGTC
CGAGACCTGC GCTTCGGGGT GGAGCATGGG GTGGACATCG TCTTTGCCTC CTTTGTGCGG
AAAGCCAGCG ACGTGGCTGC CGTCAGGGCT GCTCTGGGTC CGGAAGGACA CGGCATCAAG
ATCATCAGCA AAATTGAGAA CCACGAAGGC GTGAAGAGGT TTGATGAAAT CCTGGAGGTG
AGCGACGGCA TCATGGTGGC ACGGGGGGAC CTAGGCATCG AGATCCCAGC AGAGAAGGTT
TTCCTGGCTC AGAAGATGAT GATTGGGCGC TGCAACTTGG CGGGCAAGCC TGTTGTCTGT
GCCACACAGG TCTGGAGTGA GGCCTTGAGA TGCTGGAGAG CATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu34476
Clone ID Related Accession (Same CDS sequence) XM_006711386 , XM_006726212 , XM_011509640 , XM_011546932
Accession Version XM_011546932.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1533bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pyruvate kinase PKLR isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003315906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCAGACA CCTTCCTGGA ACACCTCTGC CTACTGGACA TTGACTCCGA GCCCGTGGCT 
GCTCGCAGTA CCAGCATCAT TGCCACCATC GGGCCAGCAT CTCGCTCCGT GGAGCGCCTC
AAGGAGATGA TCAAGGCCGG GATGAACATT GCGCGACTCA ACTTCTCCCA CGGCTCCCAC
GAGTACCATG CTGAGTCCAT CGCCAACGTC CGGGAGGCGG TGGAGAGCTT TGCAGGTTCC
CCACTCAGCT ACCGGCCCGT GGCCATCGCC CTGGACACCA AGGGACCGGA GATCCGCACT
GGGATCCTGC AGGGGGGTCC AGAGTCGGAA GTGGAGCTGG TGAAGGGCTC CCAGGTGCTG
GTGACTGTGG ACCCCGCGTT CCGGACGCGG GGGAACGCGA ACACCGTGTG GGTGGACTAC
CCCAATATTG TCCGGGTCGT GCCGGTGGGG GGCCGCATCT ACATTGACGA CGGGCTCATC
TCCCTAGTGG TCCAGAAAAT CGGCCCAGAG GGACTGGTGA CCCAAGTGGA GAACGGCGGC
GTCCTGGGCA GCCGGAAGGG CGTGAACTTG CCAGGGGCCC AGGTGGACTT GCCCGGGCTG
TCCGAGCAGG ACGTCCGAGA CCTGCGCTTC GGGGTGGAGC ATGGGGTGGA CATCGTCTTT
GCCTCCTTTG TGCGGAAAGC CAGCGACGTG GCTGCCGTCA GGGCTGCTCT GGGTCCGGAA
GGACACGGCA TCAAGATCAT CAGCAAAATT GAGAACCACG AAGGCGTGAA GAGGTTTGAT
GAAATCCTGG AGGTGAGCGA CGGCATCATG GTGGCACGGG GGGACCTAGG CATCGAGATC
CCAGCAGAGA AGGTTTTCCT GGCTCAGAAG ATGATGATTG GGCGCTGCAA CTTGGCGGGC
AAGCCTGTTG TCTGTGCCAC ACAGATGCTG GAGAGCATGA TTACCAAGCC CCGGCCAACG
AGGGCAGAGA CAAGCGATGT CGCCAATGCT GTGCTGGATG GGGCTGACTG CATCATGCTG
TCAGGGGAGA CTGCCAAGGG CAACTTCCCT GTGGAAGCGG TGAAGATGCA GCATGCGATT
GCCCGGGAGG CAGAGGCCGC AGTGTACCAC CGGCAGCTGT TTGAGGAGCT ACGTCGGGCA
GCGCCACTAA GCCGTGATCC CACTGAGGTC ACCGCCATTG GTGCTGTGGA GGCTGCCTTC
AAGTGCTGTG CTGCTGCCAT CATTGTGCTG ACCACAACTG GCCGCTCAGC CCAGCTTCTG
TCTCGGTACC GACCTCGGGC AGCAGTCATT GCTGTCACCC GCTCTGCCCA GGCTGCCCGC
CAGGTCCACT TATGCCGAGG AGTCTTCCCC TTGCTTTACC GTGAACCTCC AGAAGCCATC
TGGGCAGATG ATGTAGATCG CCGGGTGCAA TTTGGCATTG AAAGTGGAAA GCTCCGTGGC
TTCCTCCGTG TTGGAGACCT GGTGATTGTG GTGACAGGCT GGCGACCTGG CTCCGGCTAC
ACCAACATCA TGCGGGTGCT AAGCATATCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011545234.1
CDS165..1697
Misc Feature(1)225..1691(+)
Misc Feature(2)228..1682(+)
Misc Feature(3)231..1505(+)
Misc Feature(4)318..1085(+)
Translation

Target ORF information:

RefSeq Version XM_011546932
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011546932

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCAGACA CCTTCCTGGA ACACCTCTGC CTACTGGACA TTGACTCCGA GCCCGTGGCT 
GCTCGCAGTA CCAGCATCAT TGCCACCATC GGGCCAGCAT CTCGCTCCGT GGAGCGCCTC
AAGGAGATGA TCAAGGCCGG GATGAACATT GCGCGACTCA ACTTCTCCCA CGGCTCCCAC
GAGTACCATG CTGAGTCCAT CGCCAACGTC CGGGAGGCGG TGGAGAGCTT TGCAGGTTCC
CCACTCAGCT ACCGGCCCGT GGCCATCGCC CTGGACACCA AGGGACCGGA GATCCGCACT
GGGATCCTGC AGGGGGGTCC AGAGTCGGAA GTGGAGCTGG TGAAGGGCTC CCAGGTGCTG
GTGACTGTGG ACCCCGCGTT CCGGACGCGG GGGAACGCGA ACACCGTGTG GGTGGACTAC
CCCAATATTG TCCGGGTCGT GCCGGTGGGG GGCCGCATCT ACATTGACGA CGGGCTCATC
TCCCTAGTGG TCCAGAAAAT CGGCCCAGAG GGACTGGTGA CCCAAGTGGA GAACGGCGGC
GTCCTGGGCA GCCGGAAGGG CGTGAACTTG CCAGGGGCCC AGGTGGACTT GCCCGGGCTG
TCCGAGCAGG ACGTCCGAGA CCTGCGCTTC GGGGTGGAGC ATGGGGTGGA CATCGTCTTT
GCCTCCTTTG TGCGGAAAGC CAGCGACGTG GCTGCCGTCA GGGCTGCTCT GGGTCCGGAA
GGACACGGCA TCAAGATCAT CAGCAAAATT GAGAACCACG AAGGCGTGAA GAGGTTTGAT
GAAATCCTGG AGGTGAGCGA CGGCATCATG GTGGCACGGG GGGACCTAGG CATCGAGATC
CCAGCAGAGA AGGTTTTCCT GGCTCAGAAG ATGATGATTG GGCGCTGCAA CTTGGCGGGC
AAGCCTGTTG TCTGTGCCAC ACAGATGCTG GAGAGCATGA TTACCAAGCC CCGGCCAACG
AGGGCAGAGA CAAGCGATGT CGCCAATGCT GTGCTGGATG GGGCTGACTG CATCATGCTG
TCAGGGGAGA CTGCCAAGGG CAACTTCCCT GTGGAAGCGG TGAAGATGCA GCATGCGATT
GCCCGGGAGG CAGAGGCCGC AGTGTACCAC CGGCAGCTGT TTGAGGAGCT ACGTCGGGCA
GCGCCACTAA GCCGTGATCC CACTGAGGTC ACCGCCATTG GTGCTGTGGA GGCTGCCTTC
AAGTGCTGTG CTGCTGCCAT CATTGTGCTG ACCACAACTG GCCGCTCAGC CCAGCTTCTG
TCTCGGTACC GACCTCGGGC AGCAGTCATT GCTGTCACCC GCTCTGCCCA GGCTGCCCGC
CAGGTCCACT TATGCCGAGG AGTCTTCCCC TTGCTTTACC GTGAACCTCC AGAAGCCATC
TGGGCAGATG ATGTAGATCG CCGGGTGCAA TTTGGCATTG AAAGTGGAAA GCTCCGTGGC
TTCCTCCGTG TTGGAGACCT GGTGATTGTG GTGACAGGCT GGCGACCTGG CTCCGGCTAC
ACCAACATCA TGCGGGTGCT AAGCATATCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu34475
Clone ID Related Accession (Same CDS sequence) XM_005245266
Accession Version XM_005245266.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1884bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pyruvate kinase PKLR isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578801112. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGAAGGGC TGAAGTCTAG AGGTCGCGGC TGGAGGCGTC AGTGCCGGAA GCGAGCCAGG 
CTGGGAACGT CCCCGCTGTG GCTTTGGCTG CCTGTCTTAC TCAAGTTTTC ATTATCTTCC
TGGGCAGCTG ATTGGCACCC CCAGGCCCAC ACTGAAAGCA TGTCGATCCA GGAGAACATA
TCATCCCTGC AGCTTCGGTC ATGGGTCTCT AAGTCCCAAA GAGACTTAGC AAAGTCCATC
CTGATTGGGG CTCCAGGAGG GCCAGCGGGG TATCTGCGGC GGGCCAGTGT GGCCCAACTG
ACCCAGGAGC TGGGCACTGC CTTCTTCCAG CAGCAGCAGC TGCCAGCTGC TATGGCAGAC
ACCTTCCTGG AACACCTCTG CCTACTGGAC ATTGACTCCG AGCCCGTGGC TGCTCGCAGT
ACCAGCATCA TTGCCACCAT CGGGCCAGCA TCTCGCTCCG TGGAGCGCCT CAAGGAGATG
ATCAAGGCCG GGATGAACAT TGCGCGACTC AACTTCTCCC ACGGCTCCCA CGAGTACCAT
GCTGAGTCCA TCGCCAACGT CCGGGAGGCG GTGGAGAGCT TTGCAGGTTC CCCACTCAGC
TACCGGCCCG TGGCCATCGC CCTGGACACC AAGGGACCGG AGATCCGCAC TGGGATCCTG
CAGGGGGGTC CAGAGTCGGA AGTGGAGCTG GTGAAGGGCT CCCAGGTGCT GGTGACTGTG
GACCCCGCGT TCCGGACGCG GGGGAACGCG AACACCGTGT GGGTGGACTA CCCCAATATT
GTCCGGGTCG TGCCGGTGGG GGGCCGCATC TACATTGACG ACGGGCTCAT CTCCCTAGTG
GTCCAGAAAA TCGGCCCAGA GGGACTGGTG ACCCAAGTGG AGAACGGCGG CGTCCTGGGC
AGCCGGAAGG GCGTGAACTT GCCAGGGGCC CAGGTGGACT TGCCCGGGCT GTCCGAGCAG
GACGTCCGAG ACCTGCGCTT CGGGGTGGAG CATGGGGTGG ACATCGTCTT TGCCTCCTTT
GTGCGGAAAG CCAGCGACGT GGCTGCCGTC AGGGCTGCTC TGGGTCCGGA AGGACACGGC
ATCAAGATCA TCAGCAAAAT TGAGAACCAC GAAGGCGTGA AGAGGTTTGA TGAAATCCTG
GAGGTGAGCG ACGGCATCAT GGTGGCACGG GGGGACCTAG GCATCGAGAT CCCAGCAGAG
AAGGTTTTCC TGGCTCAGAA GATGATGATT GGGCGCTGCA ACTTGGCGGG CAAGCCTGTT
GTCTGTGCCA CACAGATGCT GGAGAGCATG ATTACCAAGC CCCGGCCAAC GAGGGCAGAG
ACAAGCGATG TCGCCAATGC TGTGCTGGAT GGGGCTGACT GCATCATGCT GTCAGGGGAG
ACTGCCAAGG GCAACTTCCC TGTGGAAGCG GTGAAGATGC AGCATGCGAT TGCCCGGGAG
GCAGAGGCCG CAGTGTACCA CCGGCAGCTG TTTGAGGAGC TACGTCGGGC AGCGCCACTA
AGCCGTGATC CCACTGAGGT CACCGCCATT GGTGCTGTGG AGGCTGCCTT CAAGTGCTGT
GCTGCTGCCA TCATTGTGCT GACCACAACT GGCCGCTCAG CCCAGCTTCT GTCTCGGTAC
CGACCTCGGG CAGCAGTCAT TGCTGTCACC CGCTCTGCCC AGGCTGCCCG CCAGGTCCAC
TTATGCCGAG GAGTCTTCCC CTTGCTTTAC CGTGAACCTC CAGAAGCCAT CTGGGCAGAT
GATGTAGATC GCCGGGTGCA ATTTGGCATT GAAAGTGGAA AGCTCCGTGG CTTCCTCCGT
GTTGGAGACC TGGTGATTGT GGTGACAGGC TGGCGACCTG GCTCCGGCTA CACCAACATC
ATGCGGGTGC TAAGCATATC CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005245323.1
CDS19..1902
Misc Feature(1)430..1896(+)
Misc Feature(2)433..1887(+)
Misc Feature(3)436..1710(+)
Misc Feature(4)523..1290(+)
Translation

Target ORF information:

RefSeq Version XM_005245266
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005245266

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGAAGGGC TGAAGTCTAG AGGTCGCGGC TGGAGGCGTC AGTGCCGGAA GCGAGCCAGG 
CTGGGAACGT CCCCGCTGTG GCTTTGGCTG CCTGTCTTAC TCAAGTTTTC ATTATCTTCC
TGGGCAGCTG ATTGGCACCC CCAGGCCCAC ACTGAAAGCA TGTCGATCCA GGAGAACATA
TCATCCCTGC AGCTTCGGTC ATGGGTCTCT AAGTCCCAAA GAGACTTAGC AAAGTCCATC
CTGATTGGGG CTCCAGGAGG GCCAGCGGGG TATCTGCGGC GGGCCAGTGT GGCCCAACTG
ACCCAGGAGC TGGGCACTGC CTTCTTCCAG CAGCAGCAGC TGCCAGCTGC TATGGCAGAC
ACCTTCCTGG AACACCTCTG CCTACTGGAC ATTGACTCCG AGCCCGTGGC TGCTCGCAGT
ACCAGCATCA TTGCCACCAT CGGGCCAGCA TCTCGCTCCG TGGAGCGCCT CAAGGAGATG
ATCAAGGCCG GGATGAACAT TGCGCGACTC AACTTCTCCC ACGGCTCCCA CGAGTACCAT
GCTGAGTCCA TCGCCAACGT CCGGGAGGCG GTGGAGAGCT TTGCAGGTTC CCCACTCAGC
TACCGGCCCG TGGCCATCGC CCTGGACACC AAGGGACCGG AGATCCGCAC TGGGATCCTG
CAGGGGGGTC CAGAGTCGGA AGTGGAGCTG GTGAAGGGCT CCCAGGTGCT GGTGACTGTG
GACCCCGCGT TCCGGACGCG GGGGAACGCG AACACCGTGT GGGTGGACTA CCCCAATATT
GTCCGGGTCG TGCCGGTGGG GGGCCGCATC TACATTGACG ACGGGCTCAT CTCCCTAGTG
GTCCAGAAAA TCGGCCCAGA GGGACTGGTG ACCCAAGTGG AGAACGGCGG CGTCCTGGGC
AGCCGGAAGG GCGTGAACTT GCCAGGGGCC CAGGTGGACT TGCCCGGGCT GTCCGAGCAG
GACGTCCGAG ACCTGCGCTT CGGGGTGGAG CATGGGGTGG ACATCGTCTT TGCCTCCTTT
GTGCGGAAAG CCAGCGACGT GGCTGCCGTC AGGGCTGCTC TGGGTCCGGA AGGACACGGC
ATCAAGATCA TCAGCAAAAT TGAGAACCAC GAAGGCGTGA AGAGGTTTGA TGAAATCCTG
GAGGTGAGCG ACGGCATCAT GGTGGCACGG GGGGACCTAG GCATCGAGAT CCCAGCAGAG
AAGGTTTTCC TGGCTCAGAA GATGATGATT GGGCGCTGCA ACTTGGCGGG CAAGCCTGTT
GTCTGTGCCA CACAGATGCT GGAGAGCATG ATTACCAAGC CCCGGCCAAC GAGGGCAGAG
ACAAGCGATG TCGCCAATGC TGTGCTGGAT GGGGCTGACT GCATCATGCT GTCAGGGGAG
ACTGCCAAGG GCAACTTCCC TGTGGAAGCG GTGAAGATGC AGCATGCGAT TGCCCGGGAG
GCAGAGGCCG CAGTGTACCA CCGGCAGCTG TTTGAGGAGC TACGTCGGGC AGCGCCACTA
AGCCGTGATC CCACTGAGGT CACCGCCATT GGTGCTGTGG AGGCTGCCTT CAAGTGCTGT
GCTGCTGCCA TCATTGTGCT GACCACAACT GGCCGCTCAG CCCAGCTTCT GTCTCGGTAC
CGACCTCGGG CAGCAGTCAT TGCTGTCACC CGCTCTGCCC AGGCTGCCCG CCAGGTCCAC
TTATGCCGAG GAGTCTTCCC CTTGCTTTAC CGTGAACCTC CAGAAGCCAT CTGGGCAGAT
GATGTAGATC GCCGGGTGCA ATTTGGCATT GAAAGTGGAA AGCTCCGTGG CTTCCTCCGT
GTTGGAGACC TGGTGATTGT GGTGACAGGC TGGCGACCTG GCTCCGGCTA CACCAACATC
ATGCGGGTGC TAAGCATATC CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu34476
Clone ID Related Accession (Same CDS sequence) XM_006711386 , XM_006726212 , XM_011509640 , XM_011546932
Accession Version XM_006711386.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1533bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pyruvate kinase PKLR isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578801113. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCAGACA CCTTCCTGGA ACACCTCTGC CTACTGGACA TTGACTCCGA GCCCGTGGCT 
GCTCGCAGTA CCAGCATCAT TGCCACCATC GGGCCAGCAT CTCGCTCCGT GGAGCGCCTC
AAGGAGATGA TCAAGGCCGG GATGAACATT GCGCGACTCA ACTTCTCCCA CGGCTCCCAC
GAGTACCATG CTGAGTCCAT CGCCAACGTC CGGGAGGCGG TGGAGAGCTT TGCAGGTTCC
CCACTCAGCT ACCGGCCCGT GGCCATCGCC CTGGACACCA AGGGACCGGA GATCCGCACT
GGGATCCTGC AGGGGGGTCC AGAGTCGGAA GTGGAGCTGG TGAAGGGCTC CCAGGTGCTG
GTGACTGTGG ACCCCGCGTT CCGGACGCGG GGGAACGCGA ACACCGTGTG GGTGGACTAC
CCCAATATTG TCCGGGTCGT GCCGGTGGGG GGCCGCATCT ACATTGACGA CGGGCTCATC
TCCCTAGTGG TCCAGAAAAT CGGCCCAGAG GGACTGGTGA CCCAAGTGGA GAACGGCGGC
GTCCTGGGCA GCCGGAAGGG CGTGAACTTG CCAGGGGCCC AGGTGGACTT GCCCGGGCTG
TCCGAGCAGG ACGTCCGAGA CCTGCGCTTC GGGGTGGAGC ATGGGGTGGA CATCGTCTTT
GCCTCCTTTG TGCGGAAAGC CAGCGACGTG GCTGCCGTCA GGGCTGCTCT GGGTCCGGAA
GGACACGGCA TCAAGATCAT CAGCAAAATT GAGAACCACG AAGGCGTGAA GAGGTTTGAT
GAAATCCTGG AGGTGAGCGA CGGCATCATG GTGGCACGGG GGGACCTAGG CATCGAGATC
CCAGCAGAGA AGGTTTTCCT GGCTCAGAAG ATGATGATTG GGCGCTGCAA CTTGGCGGGC
AAGCCTGTTG TCTGTGCCAC ACAGATGCTG GAGAGCATGA TTACCAAGCC CCGGCCAACG
AGGGCAGAGA CAAGCGATGT CGCCAATGCT GTGCTGGATG GGGCTGACTG CATCATGCTG
TCAGGGGAGA CTGCCAAGGG CAACTTCCCT GTGGAAGCGG TGAAGATGCA GCATGCGATT
GCCCGGGAGG CAGAGGCCGC AGTGTACCAC CGGCAGCTGT TTGAGGAGCT ACGTCGGGCA
GCGCCACTAA GCCGTGATCC CACTGAGGTC ACCGCCATTG GTGCTGTGGA GGCTGCCTTC
AAGTGCTGTG CTGCTGCCAT CATTGTGCTG ACCACAACTG GCCGCTCAGC CCAGCTTCTG
TCTCGGTACC GACCTCGGGC AGCAGTCATT GCTGTCACCC GCTCTGCCCA GGCTGCCCGC
CAGGTCCACT TATGCCGAGG AGTCTTCCCC TTGCTTTACC GTGAACCTCC AGAAGCCATC
TGGGCAGATG ATGTAGATCG CCGGGTGCAA TTTGGCATTG AAAGTGGAAA GCTCCGTGGC
TTCCTCCGTG TTGGAGACCT GGTGATTGTG GTGACAGGCT GGCGACCTGG CTCCGGCTAC
ACCAACATCA TGCGGGTGCT AAGCATATCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006711449.1
CDS122..1654
Misc Feature(1)182..1648(+)
Misc Feature(2)185..1639(+)
Misc Feature(3)188..1462(+)
Misc Feature(4)275..1042(+)
Translation

Target ORF information:

RefSeq Version XM_006711386
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006711386

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCAGACA CCTTCCTGGA ACACCTCTGC CTACTGGACA TTGACTCCGA GCCCGTGGCT 
GCTCGCAGTA CCAGCATCAT TGCCACCATC GGGCCAGCAT CTCGCTCCGT GGAGCGCCTC
AAGGAGATGA TCAAGGCCGG GATGAACATT GCGCGACTCA ACTTCTCCCA CGGCTCCCAC
GAGTACCATG CTGAGTCCAT CGCCAACGTC CGGGAGGCGG TGGAGAGCTT TGCAGGTTCC
CCACTCAGCT ACCGGCCCGT GGCCATCGCC CTGGACACCA AGGGACCGGA GATCCGCACT
GGGATCCTGC AGGGGGGTCC AGAGTCGGAA GTGGAGCTGG TGAAGGGCTC CCAGGTGCTG
GTGACTGTGG ACCCCGCGTT CCGGACGCGG GGGAACGCGA ACACCGTGTG GGTGGACTAC
CCCAATATTG TCCGGGTCGT GCCGGTGGGG GGCCGCATCT ACATTGACGA CGGGCTCATC
TCCCTAGTGG TCCAGAAAAT CGGCCCAGAG GGACTGGTGA CCCAAGTGGA GAACGGCGGC
GTCCTGGGCA GCCGGAAGGG CGTGAACTTG CCAGGGGCCC AGGTGGACTT GCCCGGGCTG
TCCGAGCAGG ACGTCCGAGA CCTGCGCTTC GGGGTGGAGC ATGGGGTGGA CATCGTCTTT
GCCTCCTTTG TGCGGAAAGC CAGCGACGTG GCTGCCGTCA GGGCTGCTCT GGGTCCGGAA
GGACACGGCA TCAAGATCAT CAGCAAAATT GAGAACCACG AAGGCGTGAA GAGGTTTGAT
GAAATCCTGG AGGTGAGCGA CGGCATCATG GTGGCACGGG GGGACCTAGG CATCGAGATC
CCAGCAGAGA AGGTTTTCCT GGCTCAGAAG ATGATGATTG GGCGCTGCAA CTTGGCGGGC
AAGCCTGTTG TCTGTGCCAC ACAGATGCTG GAGAGCATGA TTACCAAGCC CCGGCCAACG
AGGGCAGAGA CAAGCGATGT CGCCAATGCT GTGCTGGATG GGGCTGACTG CATCATGCTG
TCAGGGGAGA CTGCCAAGGG CAACTTCCCT GTGGAAGCGG TGAAGATGCA GCATGCGATT
GCCCGGGAGG CAGAGGCCGC AGTGTACCAC CGGCAGCTGT TTGAGGAGCT ACGTCGGGCA
GCGCCACTAA GCCGTGATCC CACTGAGGTC ACCGCCATTG GTGCTGTGGA GGCTGCCTTC
AAGTGCTGTG CTGCTGCCAT CATTGTGCTG ACCACAACTG GCCGCTCAGC CCAGCTTCTG
TCTCGGTACC GACCTCGGGC AGCAGTCATT GCTGTCACCC GCTCTGCCCA GGCTGCCCGC
CAGGTCCACT TATGCCGAGG AGTCTTCCCC TTGCTTTACC GTGAACCTCC AGAAGCCATC
TGGGCAGATG ATGTAGATCG CCGGGTGCAA TTTGGCATTG AAAGTGGAAA GCTCCGTGGC
TTCCTCCGTG TTGGAGACCT GGTGATTGTG GTGACAGGCT GGCGACCTGG CTCCGGCTAC
ACCAACATCA TGCGGGTGCT AAGCATATCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59731
Clone ID Related Accession (Same CDS sequence) XM_011509639
Accession Version XM_011509639.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1311bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pyruvate kinase PKLR isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGAAGGGC TGAAGTCTAG AGGTCGCGGC TGGAGGCGTC AGTGCCGGAA GCGAGCCAGG 
CTGGGAACGT CCCCGCTGTG GCTTTGGCTG CCTGTCTTAC TCAAGTTTTC ATTATCTTCC
TGGGCAGCTG ATTGGCACCC CCAGGCCCAC ACTGAAAGCA TGTCGATCCA GGAGAACATA
TCATCCCTGC AGCTTCGGTC ATGGGTCTCT AAGTCCCAAA GAGACTTAGC AAAGTCCATC
CTGATTGGGG CTCCAGGAGG GCCAGCGGGG TATCTGCGGC GGGCCAGTGT GGCCCAACTG
ACCCAGGAGC TGGGCACTGC CTTCTTCCAG CAGCAGCAGC TGCCAGCTGC TATGGCAGAC
ACCTTCCTGG AACACCTCTG CCTACTGGAC ATTGACTCCG AGCCCGTGGC TGCTCGCAGT
ACCAGCATCA TTGCCACCAT CGGGCCAGCA TCTCGCTCCG TGGAGCGCCT CAAGGAGATG
ATCAAGGCCG GGATGAACAT TGCGCGACTC AACTTCTCCC ACGGCTCCCA CGAGTACCAT
GCTGAGTCCA TCGCCAACGT CCGGGAGGCG GTGGAGAGCT TTGCAGGTTC CCCACTCAGC
TACCGGCCCG TGGCCATCGC CCTGGACACC AAGGGACCGG AGATCCGCAC TGGGATCCTG
CAGGGGGGTC CAGAGTCGGA AGTGGAGCTG GTGAAGGGCT CCCAGGTGCT GGTGACTGTG
GACCCCGCGT TCCGGACGCG GGGGAACGCG AACACCGTGT GGGTGGACTA CCCCAATATT
GTCCGGGTCG TGCCGGTGGG GGGCCGCATC TACATTGACG ACGGGCTCAT CTCCCTAGTG
GTCCAGAAAA TCGGCCCAGA GGGACTGGTG ACCCAAGTGG AGAACGGCGG CGTCCTGGGC
AGCCGGAAGG GCGTGAACTT GCCAGGGGCC CAGGTGGACT TGCCCGGGCT GTCCGAGCAG
GACGTCCGAG ACCTGCGCTT CGGGGTGGAG CATGGGGTGG ACATCGTCTT TGCCTCCTTT
GTGCGGAAAG CCAGCGACGT GGCTGCCGTC AGGGCTGCTC TGGGTCCGGA AGGACACGGC
ATCAAGATCA TCAGCAAAAT TGAGAACCAC GAAGGCGTGA AGAGGTTTGA TGAAATCCTG
GAGGTGAGCG ACGGCATCAT GGTGGCACGG GGGGACCTAG GCATCGAGAT CCCAGCAGAG
AAGGTTTTCC TGGCTCAGAA GATGATGATT GGGCGCTGCA ACTTGGCGGG CAAGCCTGTT
GTCTGTGCCA CACAGGTCTG GAGTGAGGCC TTGAGATGCT GGAGAGCATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011507941.1
CDS19..1329
Misc Feature(1)430..>1293(+)
Misc Feature(2)436..1275(+)
Misc Feature(3)523..1290(+)
Translation

Target ORF information:

RefSeq Version XM_011509639
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509639

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGAAGGGC TGAAGTCTAG AGGTCGCGGC TGGAGGCGTC AGTGCCGGAA GCGAGCCAGG 
CTGGGAACGT CCCCGCTGTG GCTTTGGCTG CCTGTCTTAC TCAAGTTTTC ATTATCTTCC
TGGGCAGCTG ATTGGCACCC CCAGGCCCAC ACTGAAAGCA TGTCGATCCA GGAGAACATA
TCATCCCTGC AGCTTCGGTC ATGGGTCTCT AAGTCCCAAA GAGACTTAGC AAAGTCCATC
CTGATTGGGG CTCCAGGAGG GCCAGCGGGG TATCTGCGGC GGGCCAGTGT GGCCCAACTG
ACCCAGGAGC TGGGCACTGC CTTCTTCCAG CAGCAGCAGC TGCCAGCTGC TATGGCAGAC
ACCTTCCTGG AACACCTCTG CCTACTGGAC ATTGACTCCG AGCCCGTGGC TGCTCGCAGT
ACCAGCATCA TTGCCACCAT CGGGCCAGCA TCTCGCTCCG TGGAGCGCCT CAAGGAGATG
ATCAAGGCCG GGATGAACAT TGCGCGACTC AACTTCTCCC ACGGCTCCCA CGAGTACCAT
GCTGAGTCCA TCGCCAACGT CCGGGAGGCG GTGGAGAGCT TTGCAGGTTC CCCACTCAGC
TACCGGCCCG TGGCCATCGC CCTGGACACC AAGGGACCGG AGATCCGCAC TGGGATCCTG
CAGGGGGGTC CAGAGTCGGA AGTGGAGCTG GTGAAGGGCT CCCAGGTGCT GGTGACTGTG
GACCCCGCGT TCCGGACGCG GGGGAACGCG AACACCGTGT GGGTGGACTA CCCCAATATT
GTCCGGGTCG TGCCGGTGGG GGGCCGCATC TACATTGACG ACGGGCTCAT CTCCCTAGTG
GTCCAGAAAA TCGGCCCAGA GGGACTGGTG ACCCAAGTGG AGAACGGCGG CGTCCTGGGC
AGCCGGAAGG GCGTGAACTT GCCAGGGGCC CAGGTGGACT TGCCCGGGCT GTCCGAGCAG
GACGTCCGAG ACCTGCGCTT CGGGGTGGAG CATGGGGTGG ACATCGTCTT TGCCTCCTTT
GTGCGGAAAG CCAGCGACGT GGCTGCCGTC AGGGCTGCTC TGGGTCCGGA AGGACACGGC
ATCAAGATCA TCAGCAAAAT TGAGAACCAC GAAGGCGTGA AGAGGTTTGA TGAAATCCTG
GAGGTGAGCG ACGGCATCAT GGTGGCACGG GGGGACCTAG GCATCGAGAT CCCAGCAGAG
AAGGTTTTCC TGGCTCAGAA GATGATGATT GGGCGCTGCA ACTTGGCGGG CAAGCCTGTT
GTCTGTGCCA CACAGGTCTG GAGTGAGGCC TTGAGATGCT GGAGAGCATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu34476
Clone ID Related Accession (Same CDS sequence) XM_006711386 , XM_006726212 , XM_011509640 , XM_011546932
Accession Version XM_011509640.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1533bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pyruvate kinase PKLR isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCAGACA CCTTCCTGGA ACACCTCTGC CTACTGGACA TTGACTCCGA GCCCGTGGCT 
GCTCGCAGTA CCAGCATCAT TGCCACCATC GGGCCAGCAT CTCGCTCCGT GGAGCGCCTC
AAGGAGATGA TCAAGGCCGG GATGAACATT GCGCGACTCA ACTTCTCCCA CGGCTCCCAC
GAGTACCATG CTGAGTCCAT CGCCAACGTC CGGGAGGCGG TGGAGAGCTT TGCAGGTTCC
CCACTCAGCT ACCGGCCCGT GGCCATCGCC CTGGACACCA AGGGACCGGA GATCCGCACT
GGGATCCTGC AGGGGGGTCC AGAGTCGGAA GTGGAGCTGG TGAAGGGCTC CCAGGTGCTG
GTGACTGTGG ACCCCGCGTT CCGGACGCGG GGGAACGCGA ACACCGTGTG GGTGGACTAC
CCCAATATTG TCCGGGTCGT GCCGGTGGGG GGCCGCATCT ACATTGACGA CGGGCTCATC
TCCCTAGTGG TCCAGAAAAT CGGCCCAGAG GGACTGGTGA CCCAAGTGGA GAACGGCGGC
GTCCTGGGCA GCCGGAAGGG CGTGAACTTG CCAGGGGCCC AGGTGGACTT GCCCGGGCTG
TCCGAGCAGG ACGTCCGAGA CCTGCGCTTC GGGGTGGAGC ATGGGGTGGA CATCGTCTTT
GCCTCCTTTG TGCGGAAAGC CAGCGACGTG GCTGCCGTCA GGGCTGCTCT GGGTCCGGAA
GGACACGGCA TCAAGATCAT CAGCAAAATT GAGAACCACG AAGGCGTGAA GAGGTTTGAT
GAAATCCTGG AGGTGAGCGA CGGCATCATG GTGGCACGGG GGGACCTAGG CATCGAGATC
CCAGCAGAGA AGGTTTTCCT GGCTCAGAAG ATGATGATTG GGCGCTGCAA CTTGGCGGGC
AAGCCTGTTG TCTGTGCCAC ACAGATGCTG GAGAGCATGA TTACCAAGCC CCGGCCAACG
AGGGCAGAGA CAAGCGATGT CGCCAATGCT GTGCTGGATG GGGCTGACTG CATCATGCTG
TCAGGGGAGA CTGCCAAGGG CAACTTCCCT GTGGAAGCGG TGAAGATGCA GCATGCGATT
GCCCGGGAGG CAGAGGCCGC AGTGTACCAC CGGCAGCTGT TTGAGGAGCT ACGTCGGGCA
GCGCCACTAA GCCGTGATCC CACTGAGGTC ACCGCCATTG GTGCTGTGGA GGCTGCCTTC
AAGTGCTGTG CTGCTGCCAT CATTGTGCTG ACCACAACTG GCCGCTCAGC CCAGCTTCTG
TCTCGGTACC GACCTCGGGC AGCAGTCATT GCTGTCACCC GCTCTGCCCA GGCTGCCCGC
CAGGTCCACT TATGCCGAGG AGTCTTCCCC TTGCTTTACC GTGAACCTCC AGAAGCCATC
TGGGCAGATG ATGTAGATCG CCGGGTGCAA TTTGGCATTG AAAGTGGAAA GCTCCGTGGC
TTCCTCCGTG TTGGAGACCT GGTGATTGTG GTGACAGGCT GGCGACCTGG CTCCGGCTAC
ACCAACATCA TGCGGGTGCT AAGCATATCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011507942.1
CDS165..1697
Misc Feature(1)225..1691(+)
Misc Feature(2)228..1682(+)
Misc Feature(3)231..1505(+)
Misc Feature(4)318..1085(+)
Translation

Target ORF information:

RefSeq Version XM_011509640
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509640

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCAGACA CCTTCCTGGA ACACCTCTGC CTACTGGACA TTGACTCCGA GCCCGTGGCT 
GCTCGCAGTA CCAGCATCAT TGCCACCATC GGGCCAGCAT CTCGCTCCGT GGAGCGCCTC
AAGGAGATGA TCAAGGCCGG GATGAACATT GCGCGACTCA ACTTCTCCCA CGGCTCCCAC
GAGTACCATG CTGAGTCCAT CGCCAACGTC CGGGAGGCGG TGGAGAGCTT TGCAGGTTCC
CCACTCAGCT ACCGGCCCGT GGCCATCGCC CTGGACACCA AGGGACCGGA GATCCGCACT
GGGATCCTGC AGGGGGGTCC AGAGTCGGAA GTGGAGCTGG TGAAGGGCTC CCAGGTGCTG
GTGACTGTGG ACCCCGCGTT CCGGACGCGG GGGAACGCGA ACACCGTGTG GGTGGACTAC
CCCAATATTG TCCGGGTCGT GCCGGTGGGG GGCCGCATCT ACATTGACGA CGGGCTCATC
TCCCTAGTGG TCCAGAAAAT CGGCCCAGAG GGACTGGTGA CCCAAGTGGA GAACGGCGGC
GTCCTGGGCA GCCGGAAGGG CGTGAACTTG CCAGGGGCCC AGGTGGACTT GCCCGGGCTG
TCCGAGCAGG ACGTCCGAGA CCTGCGCTTC GGGGTGGAGC ATGGGGTGGA CATCGTCTTT
GCCTCCTTTG TGCGGAAAGC CAGCGACGTG GCTGCCGTCA GGGCTGCTCT GGGTCCGGAA
GGACACGGCA TCAAGATCAT CAGCAAAATT GAGAACCACG AAGGCGTGAA GAGGTTTGAT
GAAATCCTGG AGGTGAGCGA CGGCATCATG GTGGCACGGG GGGACCTAGG CATCGAGATC
CCAGCAGAGA AGGTTTTCCT GGCTCAGAAG ATGATGATTG GGCGCTGCAA CTTGGCGGGC
AAGCCTGTTG TCTGTGCCAC ACAGATGCTG GAGAGCATGA TTACCAAGCC CCGGCCAACG
AGGGCAGAGA CAAGCGATGT CGCCAATGCT GTGCTGGATG GGGCTGACTG CATCATGCTG
TCAGGGGAGA CTGCCAAGGG CAACTTCCCT GTGGAAGCGG TGAAGATGCA GCATGCGATT
GCCCGGGAGG CAGAGGCCGC AGTGTACCAC CGGCAGCTGT TTGAGGAGCT ACGTCGGGCA
GCGCCACTAA GCCGTGATCC CACTGAGGTC ACCGCCATTG GTGCTGTGGA GGCTGCCTTC
AAGTGCTGTG CTGCTGCCAT CATTGTGCTG ACCACAACTG GCCGCTCAGC CCAGCTTCTG
TCTCGGTACC GACCTCGGGC AGCAGTCATT GCTGTCACCC GCTCTGCCCA GGCTGCCCGC
CAGGTCCACT TATGCCGAGG AGTCTTCCCC TTGCTTTACC GTGAACCTCC AGAAGCCATC
TGGGCAGATG ATGTAGATCG CCGGGTGCAA TTTGGCATTG AAAGTGGAAA GCTCCGTGGC
TTCCTCCGTG TTGGAGACCT GGTGATTGTG GTGACAGGCT GGCGACCTGG CTCCGGCTAC
ACCAACATCA TGCGGGTGCT AAGCATATCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26995
Clone ID Related Accession (Same CDS sequence) NM_000298
Accession Version NM_000298.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1725bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pyruvate kinase PKLR isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB015983.1, BC025737.1, AL713999.28 and BF110802.1. This sequence is a reference standard in the RefSeqGene project. On May 28, 2008 this sequence version replaced gi:82659098. Transcript Variant: This variant (1) encodes the longer isoform (1). Isoform 1 is also known as type R. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: community standard (PMID:17360088) gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AB015983.1, BC025737.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA1970526 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGTCGATCC AGGAGAACAT ATCATCCCTG CAGCTTCGGT CATGGGTCTC TAAGTCCCAA 
AGAGACTTAG CAAAGTCCAT CCTGATTGGG GCTCCAGGAG GGCCAGCGGG GTATCTGCGG
CGGGCCAGTG TGGCCCAACT GACCCAGGAG CTGGGCACTG CCTTCTTCCA GCAGCAGCAG
CTGCCAGCTG CTATGGCAGA CACCTTCCTG GAACACCTCT GCCTACTGGA CATTGACTCC
GAGCCCGTGG CTGCTCGCAG TACCAGCATC ATTGCCACCA TCGGGCCAGC ATCTCGCTCC
GTGGAGCGCC TCAAGGAGAT GATCAAGGCC GGGATGAACA TTGCGCGACT CAACTTCTCC
CACGGCTCCC ACGAGTACCA TGCTGAGTCC ATCGCCAACG TCCGGGAGGC GGTGGAGAGC
TTTGCAGGTT CCCCACTCAG CTACCGGCCC GTGGCCATCG CCCTGGACAC CAAGGGACCG
GAGATCCGCA CTGGGATCCT GCAGGGGGGT CCAGAGTCGG AAGTGGAGCT GGTGAAGGGC
TCCCAGGTGC TGGTGACTGT GGACCCCGCG TTCCGGACGC GGGGGAACGC GAACACCGTG
TGGGTGGACT ACCCCAATAT TGTCCGGGTC GTGCCGGTGG GGGGCCGCAT CTACATTGAC
GACGGGCTCA TCTCCCTAGT GGTCCAGAAA ATCGGCCCAG AGGGACTGGT GACCCAAGTG
GAGAACGGCG GCGTCCTGGG CAGCCGGAAG GGCGTGAACT TGCCAGGGGC CCAGGTGGAC
TTGCCCGGGC TGTCCGAGCA GGACGTCCGA GACCTGCGCT TCGGGGTGGA GCATGGGGTG
GACATCGTCT TTGCCTCCTT TGTGCGGAAA GCCAGCGACG TGGCTGCCGT CAGGGCTGCT
CTGGGTCCGG AAGGACACGG CATCAAGATC ATCAGCAAAA TTGAGAACCA CGAAGGCGTG
AAGAGGTTTG ATGAAATCCT GGAGGTGAGC GACGGCATCA TGGTGGCACG GGGGGACCTA
GGCATCGAGA TCCCAGCAGA GAAGGTTTTC CTGGCTCAGA AGATGATGAT TGGGCGCTGC
AACTTGGCGG GCAAGCCTGT TGTCTGTGCC ACACAGATGC TGGAGAGCAT GATTACCAAG
CCCCGGCCAA CGAGGGCAGA GACAAGCGAT GTCGCCAATG CTGTGCTGGA TGGGGCTGAC
TGCATCATGC TGTCAGGGGA GACTGCCAAG GGCAACTTCC CTGTGGAAGC GGTGAAGATG
CAGCATGCGA TTGCCCGGGA GGCAGAGGCC GCAGTGTACC ACCGGCAGCT GTTTGAGGAG
CTACGTCGGG CAGCGCCACT AAGCCGTGAT CCCACTGAGG TCACCGCCAT TGGTGCTGTG
GAGGCTGCCT TCAAGTGCTG TGCTGCTGCC ATCATTGTGC TGACCACAAC TGGCCGCTCA
GCCCAGCTTC TGTCTCGGTA CCGACCTCGG GCAGCAGTCA TTGCTGTCAC CCGCTCTGCC
CAGGCTGCCC GCCAGGTCCA CTTATGCCGA GGAGTCTTCC CCTTGCTTTA CCGTGAACCT
CCAGAAGCCA TCTGGGCAGA TGATGTAGAT CGCCGGGTGC AATTTGGCAT TGAAAGTGGA
AAGCTCCGTG GCTTCCTCCG TGTTGGAGAC CTGGTGATTG TGGTGACAGG CTGGCGACCT
GGCTCCGGCT ACACCAACAT CATGCGGGTG CTAAGCATAT CCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000289.1
CDS40..1764
Misc Feature(1)292..1758(+)
Misc Feature(2)295..1749(+)
Misc Feature(3)298..1572(+)
Misc Feature(4)385..1152(+)
Misc Feature(5)976..978(+)
Misc Feature(6)1462..1479(+)
Misc Feature(7)1705..1707(+)
Misc Feature(8)1714..1731(+)
Exon (1)1..139
Gene:PKLR
Gene Synonym:
Exon (2)140..322
Gene:PKLR
Gene Synonym:
Exon (3)323..414
Gene:PKLR
Gene Synonym:
Exon (4)415..546
Gene:PKLR
Gene Synonym:
Exon (5)547..733
Gene:PKLR
Gene Synonym:
Exon (6)734..1004
Gene:PKLR
Gene Synonym:
Exon (7)1005..1155
Gene:PKLR
Gene Synonym:
Exon (8)1156..1308
Gene:PKLR
Gene Synonym:
Exon (9)1309..1475
Gene:PKLR
Gene Synonym:
Exon (10)1476..1657
Gene:PKLR
Gene Synonym:
Exon (11)1658..3043
Gene:PKLR
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000298
Organism Homo sapiens (human)
Definition Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000298

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGTCGATCC AGGAGAACAT ATCATCCCTG CAGCTTCGGT CATGGGTCTC TAAGTCCCAA 
AGAGACTTAG CAAAGTCCAT CCTGATTGGG GCTCCAGGAG GGCCAGCGGG GTATCTGCGG
CGGGCCAGTG TGGCCCAACT GACCCAGGAG CTGGGCACTG CCTTCTTCCA GCAGCAGCAG
CTGCCAGCTG CTATGGCAGA CACCTTCCTG GAACACCTCT GCCTACTGGA CATTGACTCC
GAGCCCGTGG CTGCTCGCAG TACCAGCATC ATTGCCACCA TCGGGCCAGC ATCTCGCTCC
GTGGAGCGCC TCAAGGAGAT GATCAAGGCC GGGATGAACA TTGCGCGACT CAACTTCTCC
CACGGCTCCC ACGAGTACCA TGCTGAGTCC ATCGCCAACG TCCGGGAGGC GGTGGAGAGC
TTTGCAGGTT CCCCACTCAG CTACCGGCCC GTGGCCATCG CCCTGGACAC CAAGGGACCG
GAGATCCGCA CTGGGATCCT GCAGGGGGGT CCAGAGTCGG AAGTGGAGCT GGTGAAGGGC
TCCCAGGTGC TGGTGACTGT GGACCCCGCG TTCCGGACGC GGGGGAACGC GAACACCGTG
TGGGTGGACT ACCCCAATAT TGTCCGGGTC GTGCCGGTGG GGGGCCGCAT CTACATTGAC
GACGGGCTCA TCTCCCTAGT GGTCCAGAAA ATCGGCCCAG AGGGACTGGT GACCCAAGTG
GAGAACGGCG GCGTCCTGGG CAGCCGGAAG GGCGTGAACT TGCCAGGGGC CCAGGTGGAC
TTGCCCGGGC TGTCCGAGCA GGACGTCCGA GACCTGCGCT TCGGGGTGGA GCATGGGGTG
GACATCGTCT TTGCCTCCTT TGTGCGGAAA GCCAGCGACG TGGCTGCCGT CAGGGCTGCT
CTGGGTCCGG AAGGACACGG CATCAAGATC ATCAGCAAAA TTGAGAACCA CGAAGGCGTG
AAGAGGTTTG ATGAAATCCT GGAGGTGAGC GACGGCATCA TGGTGGCACG GGGGGACCTA
GGCATCGAGA TCCCAGCAGA GAAGGTTTTC CTGGCTCAGA AGATGATGAT TGGGCGCTGC
AACTTGGCGG GCAAGCCTGT TGTCTGTGCC ACACAGATGC TGGAGAGCAT GATTACCAAG
CCCCGGCCAA CGAGGGCAGA GACAAGCGAT GTCGCCAATG CTGTGCTGGA TGGGGCTGAC
TGCATCATGC TGTCAGGGGA GACTGCCAAG GGCAACTTCC CTGTGGAAGC GGTGAAGATG
CAGCATGCGA TTGCCCGGGA GGCAGAGGCC GCAGTGTACC ACCGGCAGCT GTTTGAGGAG
CTACGTCGGG CAGCGCCACT AAGCCGTGAT CCCACTGAGG TCACCGCCAT TGGTGCTGTG
GAGGCTGCCT TCAAGTGCTG TGCTGCTGCC ATCATTGTGC TGACCACAAC TGGCCGCTCA
GCCCAGCTTC TGTCTCGGTA CCGACCTCGG GCAGCAGTCA TTGCTGTCAC CCGCTCTGCC
CAGGCTGCCC GCCAGGTCCA CTTATGCCGA GGAGTCTTCC CCTTGCTTTA CCGTGAACCT
CCAGAAGCCA TCTGGGCAGA TGATGTAGAT CGCCGGGTGC AATTTGGCAT TGAAAGTGGA
AAGCTCCGTG GCTTCCTCCG TGTTGGAGAC CTGGTGATTG TGGTGACAGG CTGGCGACCT
GGCTCCGGCT ACACCAACAT CATGCGGGTG CTAAGCATAT CCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27268
Clone ID Related Accession (Same CDS sequence) NM_181871
Accession Version NM_181871.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1632bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pyruvate kinase PKLR isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK225947.1, M15465.1, AL713999.28 and BF110802.1. On May 28, 2008 this sequence version replaced gi:82659100. Transcript Variant: This variant (2) uses an alternate 5' exon, as compared to variant 1. The resulting isoform (2) has a distinct and shorter N-terminus, as compared to isoform 1. Isoform 2 is also known as type L. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:17360088) gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK225947.1, M15465.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA1970526 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGAAGGGC CAGCGGGGTA TCTGCGGCGG GCCAGTGTGG CCCAACTGAC CCAGGAGCTG 
GGCACTGCCT TCTTCCAGCA GCAGCAGCTG CCAGCTGCTA TGGCAGACAC CTTCCTGGAA
CACCTCTGCC TACTGGACAT TGACTCCGAG CCCGTGGCTG CTCGCAGTAC CAGCATCATT
GCCACCATCG GGCCAGCATC TCGCTCCGTG GAGCGCCTCA AGGAGATGAT CAAGGCCGGG
ATGAACATTG CGCGACTCAA CTTCTCCCAC GGCTCCCACG AGTACCATGC TGAGTCCATC
GCCAACGTCC GGGAGGCGGT GGAGAGCTTT GCAGGTTCCC CACTCAGCTA CCGGCCCGTG
GCCATCGCCC TGGACACCAA GGGACCGGAG ATCCGCACTG GGATCCTGCA GGGGGGTCCA
GAGTCGGAAG TGGAGCTGGT GAAGGGCTCC CAGGTGCTGG TGACTGTGGA CCCCGCGTTC
CGGACGCGGG GGAACGCGAA CACCGTGTGG GTGGACTACC CCAATATTGT CCGGGTCGTG
CCGGTGGGGG GCCGCATCTA CATTGACGAC GGGCTCATCT CCCTAGTGGT CCAGAAAATC
GGCCCAGAGG GACTGGTGAC CCAAGTGGAG AACGGCGGCG TCCTGGGCAG CCGGAAGGGC
GTGAACTTGC CAGGGGCCCA GGTGGACTTG CCCGGGCTGT CCGAGCAGGA CGTCCGAGAC
CTGCGCTTCG GGGTGGAGCA TGGGGTGGAC ATCGTCTTTG CCTCCTTTGT GCGGAAAGCC
AGCGACGTGG CTGCCGTCAG GGCTGCTCTG GGTCCGGAAG GACACGGCAT CAAGATCATC
AGCAAAATTG AGAACCACGA AGGCGTGAAG AGGTTTGATG AAATCCTGGA GGTGAGCGAC
GGCATCATGG TGGCACGGGG GGACCTAGGC ATCGAGATCC CAGCAGAGAA GGTTTTCCTG
GCTCAGAAGA TGATGATTGG GCGCTGCAAC TTGGCGGGCA AGCCTGTTGT CTGTGCCACA
CAGATGCTGG AGAGCATGAT TACCAAGCCC CGGCCAACGA GGGCAGAGAC AAGCGATGTC
GCCAATGCTG TGCTGGATGG GGCTGACTGC ATCATGCTGT CAGGGGAGAC TGCCAAGGGC
AACTTCCCTG TGGAAGCGGT GAAGATGCAG CATGCGATTG CCCGGGAGGC AGAGGCCGCA
GTGTACCACC GGCAGCTGTT TGAGGAGCTA CGTCGGGCAG CGCCACTAAG CCGTGATCCC
ACTGAGGTCA CCGCCATTGG TGCTGTGGAG GCTGCCTTCA AGTGCTGTGC TGCTGCCATC
ATTGTGCTGA CCACAACTGG CCGCTCAGCC CAGCTTCTGT CTCGGTACCG ACCTCGGGCA
GCAGTCATTG CTGTCACCCG CTCTGCCCAG GCTGCCCGCC AGGTCCACTT ATGCCGAGGA
GTCTTCCCCT TGCTTTACCG TGAACCTCCA GAAGCCATCT GGGCAGATGA TGTAGATCGC
CGGGTGCAAT TTGGCATTGA AAGTGGAAAG CTCCGTGGCT TCCTCCGTGT TGGAGACCTG
GTGATTGTGG TGACAGGCTG GCGACCTGGC TCCGGCTACA CCAACATCAT GCGGGTGCTA
AGCATATCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_870986.1
CDS115..1746
Misc Feature(1)22..24(+)
Misc Feature(2)274..1740(+)
Misc Feature(3)277..1731(+)
Misc Feature(4)280..1554(+)
Misc Feature(5)367..1134(+)
Misc Feature(6)1687..1689(+)
Exon (1)1..121
Gene:PKLR
Gene Synonym:
Exon (2)122..304
Gene:PKLR
Gene Synonym:
Exon (3)305..396
Gene:PKLR
Gene Synonym:
Exon (4)397..528
Gene:PKLR
Gene Synonym:
Exon (5)529..715
Gene:PKLR
Gene Synonym:
Exon (6)716..986
Gene:PKLR
Gene Synonym:
Exon (7)987..1137
Gene:PKLR
Gene Synonym:
Exon (8)1138..1290
Gene:PKLR
Gene Synonym:
Exon (9)1291..1457
Gene:PKLR
Gene Synonym:
Exon (10)1458..1639
Gene:PKLR
Gene Synonym:
Exon (11)1640..3025
Gene:PKLR
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_181871
Organism Homo sapiens (human)
Definition Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_181871

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGAAGGGC CAGCGGGGTA TCTGCGGCGG GCCAGTGTGG CCCAACTGAC CCAGGAGCTG 
GGCACTGCCT TCTTCCAGCA GCAGCAGCTG CCAGCTGCTA TGGCAGACAC CTTCCTGGAA
CACCTCTGCC TACTGGACAT TGACTCCGAG CCCGTGGCTG CTCGCAGTAC CAGCATCATT
GCCACCATCG GGCCAGCATC TCGCTCCGTG GAGCGCCTCA AGGAGATGAT CAAGGCCGGG
ATGAACATTG CGCGACTCAA CTTCTCCCAC GGCTCCCACG AGTACCATGC TGAGTCCATC
GCCAACGTCC GGGAGGCGGT GGAGAGCTTT GCAGGTTCCC CACTCAGCTA CCGGCCCGTG
GCCATCGCCC TGGACACCAA GGGACCGGAG ATCCGCACTG GGATCCTGCA GGGGGGTCCA
GAGTCGGAAG TGGAGCTGGT GAAGGGCTCC CAGGTGCTGG TGACTGTGGA CCCCGCGTTC
CGGACGCGGG GGAACGCGAA CACCGTGTGG GTGGACTACC CCAATATTGT CCGGGTCGTG
CCGGTGGGGG GCCGCATCTA CATTGACGAC GGGCTCATCT CCCTAGTGGT CCAGAAAATC
GGCCCAGAGG GACTGGTGAC CCAAGTGGAG AACGGCGGCG TCCTGGGCAG CCGGAAGGGC
GTGAACTTGC CAGGGGCCCA GGTGGACTTG CCCGGGCTGT CCGAGCAGGA CGTCCGAGAC
CTGCGCTTCG GGGTGGAGCA TGGGGTGGAC ATCGTCTTTG CCTCCTTTGT GCGGAAAGCC
AGCGACGTGG CTGCCGTCAG GGCTGCTCTG GGTCCGGAAG GACACGGCAT CAAGATCATC
AGCAAAATTG AGAACCACGA AGGCGTGAAG AGGTTTGATG AAATCCTGGA GGTGAGCGAC
GGCATCATGG TGGCACGGGG GGACCTAGGC ATCGAGATCC CAGCAGAGAA GGTTTTCCTG
GCTCAGAAGA TGATGATTGG GCGCTGCAAC TTGGCGGGCA AGCCTGTTGT CTGTGCCACA
CAGATGCTGG AGAGCATGAT TACCAAGCCC CGGCCAACGA GGGCAGAGAC AAGCGATGTC
GCCAATGCTG TGCTGGATGG GGCTGACTGC ATCATGCTGT CAGGGGAGAC TGCCAAGGGC
AACTTCCCTG TGGAAGCGGT GAAGATGCAG CATGCGATTG CCCGGGAGGC AGAGGCCGCA
GTGTACCACC GGCAGCTGTT TGAGGAGCTA CGTCGGGCAG CGCCACTAAG CCGTGATCCC
ACTGAGGTCA CCGCCATTGG TGCTGTGGAG GCTGCCTTCA AGTGCTGTGC TGCTGCCATC
ATTGTGCTGA CCACAACTGG CCGCTCAGCC CAGCTTCTGT CTCGGTACCG ACCTCGGGCA
GCAGTCATTG CTGTCACCCG CTCTGCCCAG GCTGCCCGCC AGGTCCACTT ATGCCGAGGA
GTCTTCCCCT TGCTTTACCG TGAACCTCCA GAAGCCATCT GGGCAGATGA TGTAGATCGC
CGGGTGCAAT TTGGCATTGA AAGTGGAAAG CTCCGTGGCT TCCTCCGTGT TGGAGACCTG
GTGATTGTGG TGACAGGCTG GCGACCTGGC TCCGGCTACA CCAACATCAT GCGGGTGCTA
AGCATATCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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