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PLAGL1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol PLAGL1
Entrez Gene ID 5325
Full Name pleiomorphic adenoma gene-like 1
Synonyms LOT1, ZAC, ZAC1
General protein information
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6q24-q25

Summary This gene encodes a C2H2 zinc finger protein with transactivation and DNA-binding activities. It has been shown to have anti-proliferative properties, and thus thought to function as a tumor suppressor. In addition, overexpression of this gene during fetal development is believed to underlie the rare disorder, transient neonatal diabetes mellitus (TNDM). This gene is imprinted, with preferential expression of the paternal allele in many tissues, however, biallelic expression has been noted in peripheral blood leucocytes. A recent study reports that tissue-specific imprinting results from variable utilization of monoallelic and biallelic promoters. Many transcript variants differing in the 5' UTR and encoding two different isoforms, have been found for this gene. [provided by RefSeq, Oct 2010]. lac of sum
Disorder MIM:

603044

Disorder Html: {Diabetes mellitus, transient neonatal}, 601410 (1)





The following PLAGL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLAGL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19788 NM_002656 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19858 NM_006718 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19858 NM_001080951 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19858 NM_001080952 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19858 NM_001080953 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19858 NM_001080954 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19788 NM_001080955 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19788 NM_001080956 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19788 NM_001289037 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19788 NM_001289038 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19788 NM_001289039 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19788 NM_001289040 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19788 NM_001289041 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 21, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19858 NM_001289042 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19858 NM_001289043 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19858 NM_001289044 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 15, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19858 NM_001289045 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 16, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19858 NM_001289046 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 17, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19858 NM_001289047 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 18, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19858 NM_001289048 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 19, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu19858 NM_001289049 Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 20, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu19788
Accession Version NM_002656.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1236bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product zinc finger protein PLAGL1 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA446007.1, DA549753.1, DA391124.1, AJ311395.1 and AI741398.1. On Feb 2, 2007 this sequence version replaced gi:37622889. Summary: This gene encodes a C2H2 zinc finger protein with transactivation and DNA-binding activities. It has been shown to have anti-proliferative properties, and thus thought to function as a tumor suppressor. In addition, overexpression of this gene during fetal development is believed to underlie the rare disorder, transient neonatal diabetes mellitus (TNDM). This gene is imprinted, with preferential expression of the paternal allele in many tissues, however, biallelic expression has been noted in peripheral blood leucocytes. A recent study reports that tissue-specific imprinting results from variable utilization of monoallelic and biallelic promoters. Many transcript variants differing in the 5' UTR and encoding two different isoforms, have been found for this gene. [provided by RefSeq, Oct 2010]. Transcript Variant: This variant (1) encodes the shorter isoform (1). Variants 1, 7, 8, 9 11, 13, 14 and 21 all encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## imprinted gene :: PMID: 17341487, 10655556 ##RefSeq-Attributes-END## ##Evidence-Data-START## CDS exon combination :: U81992.1, AJ303119.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2162568 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCTACCC ATTCTCCCCA GAAATCTCAC CAGTGTGCTC ACTGTGAGAA GACGTTCAAC 
CGGAAAGACC ACCTGAAAAA CCACCTCCAG ACCCACGACC CCAACAAAAT GGCCTTTGGG
TGTGAGGAGT GTGGGAAGAA GTACAACACC ATGCTGGGCT ATAAGAGGCA CCTGGCCCTC
CATGCGGCCA GCAGTGGGGA CCTCACCTGT GGGGTCTGTG CCCTGGAGCT AGGGAGCACC
GAGGTGCTAC TGGACCACCT CAAAGCCCAT GCGGAAGAGA AGCCCCCTAG CGGAACCAAG
GAAAAGAAGC ACCAGTGCGA CCACTGTGAA AGATGCTTCT ACACCCGGAA GGATGTGCGA
CGCCACCTGG TGGTCCACAC AGGATGCAAG GACTTCCTGT GCCAGTTCTG TGCCCAGAGA
TTTGGGCGCA AGGATCACCT CACCCGGCAT ACCAAGAAGA CCCACTCACA GGAGCTGATG
AAAGAGAGCT TGCAGACCGG AGACCTTCTG AGCACCTTCC ACACCATCTC GCCTTCATTC
CAACTGAAGG CTGCTGCCTT GCCTCCTTTC CCTTTAGGAG CTTCTGCCCA GAACGGGCTT
GCAAGTAGCT TGCCAGCTGA GGTCCATAGC CTCACCCTCA GTCCCCCAGA ACAAGCCGCC
CAGCCTATGC AGCCGCTGCC AGAGTCCCTG GCCTCCCTCC ACCCCTCGGT ATCCCCTGGC
TCTCCTCCGC CACCCCTTCC CAATCACAAG TACAACACCA CTTCTACCTC ATACTCCCCA
CTTGCAAGCC TGCCCCTCAA AGCAGATACT AAAGGTTTTT GCAATATCAG TTTGTTTGAG
GACTTGCCTC TGCAAGAGCC TCAGTCACCT CAAAAGCTCA ACCCAGGTTT TGATCTGGCT
AAGGGAAATG CTGGTAAAGT AAACCTGCCC AAGGAGCTGC CTGCAGATGC TGTGAACCTA
ACAATACCTG CCTCTCTGGA CCTGTCCCCC CTGTTGGGCT TCTGGCAGCT GCCCCCTCCT
GCTACCCAAA ATACCTTTGG GAATAGCACT CTTGCCCTGG GGCCTGGGGA ATCTTTGCCC
CACAGGTTAA GCTGTCTGGG GCAGCAGCAG CAAGAACCCC CACTTGCCAT GGGCACTGTG
AGCCTGGGCC AGCTCCCCCT GCCCCCCATC CCTCATGTGT TCTCAGCTGG CACTGGCTCT
GCCATCCTGC CTCATTTCCA TCATGCATTC AGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_002647.2
CDS585..1820
Misc Feature(1)510..512(+)
Misc Feature(2)591..764(+)
Misc Feature(3)612..680(+)
Misc Feature(4)618..680(+)
Misc Feature(5)618..677(+)
Misc Feature(6)705..767(+)
Misc Feature(7)705..767(+)
Misc Feature(8)792..854(+)
Misc Feature(9)792..854(+)
Misc Feature(10)807..1046(+)
Misc Feature(11)900..962(+)
Misc Feature(12)900..959(+)
Misc Feature(13)984..1049(+)
Misc Feature(14)984..1049(+)
Exon (1)1..315
Gene:PLAGL1
Gene Synonym:
Exon (2)316..387
Gene:PLAGL1
Gene Synonym:
Exon (3)388..460
Gene:PLAGL1
Gene Synonym:
Exon (4)461..492
Gene:PLAGL1
Gene Synonym:
Exon (5)493..580
Gene:PLAGL1
Gene Synonym:
Exon (6)581..2944
Gene:PLAGL1
Gene Synonym:
Translation
Position Chain Variation Link
98 98 a, g dbSNP:549107424
130 130 a, t dbSNP:188083405
180 180 a, g dbSNP:113189219
264 264 a, g dbSNP:779493627
279 279 a, g dbSNP:67300369
287 287 c, t dbSNP:749380636
368 368 a, t dbSNP:543763464
381 381 c, t dbSNP:573299088
413 413 a, g dbSNP:554648181
421 421 c, t dbSNP:192562088
434 434 a, g dbSNP:368424368
469 469 a, t dbSNP:370220800
540 540 c, t dbSNP:553367951
541 541 a, c, g dbSNP:570633358
553 553 a, g dbSNP:551127170
592 592 a, c dbSNP:758281178
626 626 c, t dbSNP:745781639
634 634 -, aga dbSNP:776571919
638 638 a, g dbSNP:144954752
645 645 c, t dbSNP:757324170
646 646 a, g dbSNP:753924230
680 680 c, t dbSNP:764320213
681 681 a, g, t dbSNP:753068252
688 688 a, g dbSNP:768018756
689 689 a, c dbSNP:760031131
691 691 -, caa dbSNP:766108577
695 695 a, g dbSNP:774971840
703 703 a, g dbSNP:139967852
708 708 a, g dbSNP:767063830
710 710 a, g dbSNP:761345864
725 725 a, g dbSNP:776006127
729 729 a, c dbSNP:768311718
730 730 a, c dbSNP:746660705
752 752 a, g dbSNP:775255229
769 769 a, c, t dbSNP:745728602
770 770 a, g dbSNP:778809798
774 774 a, c dbSNP:757200008
778 778 a, g dbSNP:749325083
790 790 c, t dbSNP:201937527
795 795 g, t dbSNP:201753929
797 797 g, t dbSNP:780620100
815 815 a, g dbSNP:368641715
817 817 c, g dbSNP:755394360
819 819 a, g dbSNP:151100437
823 823 c, g dbSNP:766922660
824 824 c, t dbSNP:142899386
828 828 c, g dbSNP:148432028
830 830 g, t dbSNP:763488544
833 833 a, c dbSNP:374451635
834 834 c, t dbSNP:538240247
835 835 c, t dbSNP:771769935
841 841 a, t dbSNP:759269789
849 849 g, t dbSNP:17847327
857 857 a, g dbSNP:17073229
860 860 a, g, t dbSNP:777804198
867 867 a, c dbSNP:769737512
871 871 c, t dbSNP:748313279
872 872 a, t dbSNP:781556516
875 875 c, t dbSNP:755267953
876 876 a, g dbSNP:751926904
903 903 a, g dbSNP:780599925
907 907 a, g dbSNP:758839660
931 931 a, g dbSNP:750874987
935 935 a, g dbSNP:765832081
943 943 a, g dbSNP:760099365
953 953 g, t dbSNP:752208274
956 956 a, g dbSNP:267600848
998 998 c, t dbSNP:767098050
1013 1013 c, t dbSNP:141784122
1018 1018 a, t dbSNP:1063413
1034 1034 c, t dbSNP:36120645
1046 1046 a, c dbSNP:547586757
1055 1055 a, g dbSNP:762851758
1056 1056 -, gag dbSNP:760478312
1063 1063 c, t dbSNP:773005838
1069 1069 a, g dbSNP:34652819
1071 1071 a, g dbSNP:748123821
1072 1072 c, g dbSNP:781448211
1079 1079 c, g dbSNP:375679973
1082 1082 c, g, t dbSNP:370449852
1083 1083 a, g dbSNP:758678527
1086 1086 a, g dbSNP:565181543
1089 1089 a, c dbSNP:201977118
1093 1093 c, t dbSNP:377135913
1095 1095 a, c dbSNP:752153135
1107 1107 a, g dbSNP:17847328
1110 1110 a, t dbSNP:754521531
1114 1114 c, t dbSNP:751187671
1120 1120 c, t dbSNP:766110989
1128 1128 -, c dbSNP:35411045
1137 1137 a, g dbSNP:560576837
1145 1145 a, g dbSNP:373156842
1147 1147 c, g dbSNP:142705655
1155 1155 c, t dbSNP:761877385
1168 1168 c, g dbSNP:776753534
1169 1169 c, t dbSNP:768835975
1179 1179 a, g dbSNP:372873338
1180 1180 a, g dbSNP:747145351
1197 1197 c, t dbSNP:775851406
1201 1201 c, g dbSNP:369453748
1203 1203 a, g dbSNP:746160419
1211 1211 c, t dbSNP:200072882
1219 1219 c, t dbSNP:757664610
1220 1220 c, t dbSNP:749761427
1236 1236 a, c dbSNP:778298992
1241 1241 c, t dbSNP:149291080
1242 1242 a, c, g dbSNP:765875826
1243 1243 c, t dbSNP:35263016
1253 1253 a, g dbSNP:578145229
1258 1258 c, t dbSNP:377306571
1259 1259 a, g, t dbSNP:776698636
1265 1265 a, g dbSNP:764195119
1266 1266 c, g dbSNP:141235151
1267 1267 a, c dbSNP:775653923
1268 1268 -, ccagag dbSNP:774596210
1268 1268 a, g dbSNP:772136403
1274 1274 a, g dbSNP:62427100
1275 1275 a, g dbSNP:780626452
1280 1280 c, g dbSNP:374462557
1284 1284 a, c dbSNP:774476162
1286 1286 c, t dbSNP:368929894
1290 1290 c, t dbSNP:556887561
1291 1291 c, t dbSNP:749708141
1292 1292 a, g, t dbSNP:201057231
1293 1293 a, g dbSNP:746453740
1297 1297 c, t dbSNP:779649831
1298 1298 c, t dbSNP:757889837
1304 1304 c, t dbSNP:750070946
1312 1312 c, t dbSNP:200573380
1313 1313 a, g dbSNP:17847330
1327 1327 a, t dbSNP:371698312
1339 1339 a, c dbSNP:147054773
1343 1343 c, t dbSNP:200036472
1344 1344 a, g dbSNP:752677729
1345 1345 c, t dbSNP:767601739
1355 1355 a, t dbSNP:759582013
1362 1362 c, t dbSNP:774617149
1363 1363 c, t dbSNP:771206023
1368 1368 a, g, t dbSNP:141627165
1369 1369 a, c dbSNP:770249452
1376 1376 a, c, g dbSNP:147885532
1379 1379 a, c dbSNP:771601130
1380 1380 -, c dbSNP:768840283
1381 1381 a, t dbSNP:745438962
1383 1383 a, c dbSNP:778643791
1404 1404 c, t dbSNP:756984299
1408 1408 a, g dbSNP:374631467
1418 1418 g, t dbSNP:777741438
1425 1425 a, g dbSNP:756012523
1430 1430 a, g dbSNP:752655598
1451 1451 a, t dbSNP:138688804
1463 1463 c, g dbSNP:759653376
1471 1471 a, g dbSNP:751593481
1479 1479 c, g dbSNP:766525788
1481 1481 a, g dbSNP:763180631
1489 1489 a, g dbSNP:773466984
1524 1524 a, c dbSNP:372153249
1540 1540 a, c dbSNP:762144772
1546 1546 a, c dbSNP:777194150
1549 1549 c, t dbSNP:769049757
1550 1550 a, c dbSNP:200257642
1551 1551 c, t dbSNP:774021173
1560 1560 c, g dbSNP:201957325
1562 1562 a, g dbSNP:72546304
1565 1565 c, t dbSNP:145034248
1568 1568 c, g dbSNP:755923312
1570 1570 a, c, g, t dbSNP:754880138
1571 1571 a, c, g dbSNP:145386261
1572 1572 c, g dbSNP:758526896
1574 1574 a, c, g, t dbSNP:150039945
1575 1575 a, c, g dbSNP:764612601
1576 1576 c, t dbSNP:149400255
1577 1577 c, g dbSNP:761118576
1598 1598 a, c, t dbSNP:17847331
1601 1601 c, t dbSNP:748862556
1606 1606 c, t dbSNP:772992931
1610 1610 c, t dbSNP:769332223
1614 1614 a, c dbSNP:747916100
1615 1615 a, c dbSNP:780866537
1617 1617 a, g dbSNP:751709706
1621 1621 c, t dbSNP:754901227
1627 1627 a, g dbSNP:373757589
1629 1629 a, g dbSNP:780028654
1638 1638 a, g dbSNP:758471604
1639 1639 c, t dbSNP:750509016
1646 1646 a, g dbSNP:765442934
1647 1647 -, cctgggg dbSNP:749424025
1647 1647 a, c dbSNP:757445503
1650 1650 a, g dbSNP:754192424
1655 1655 a, c dbSNP:370253986
1659 1659 c, t dbSNP:548577685
1674 1674 a, c dbSNP:776125291
1675 1675 a, g dbSNP:764191640
1681 1681 a, t dbSNP:768204588
1682 1682 a, g dbSNP:762482586
1685 1685 g, t dbSNP:772859501
1687 1687 a, g dbSNP:769435521
1693 1693 a, c dbSNP:374421447
1694 1694 g, t dbSNP:776397265
1696 1696 -, gca dbSNP:775540928
1698 1698 -, c dbSNP:35716705
1702 1702 c, t dbSNP:768326522
1713 1713 a, g dbSNP:746874822
1715 1715 a, c, g dbSNP:527350024
1731 1731 a, g dbSNP:745900352
1751 1751 c, t dbSNP:778890400
1756 1756 a, c dbSNP:36087014
1759 1759 a, g dbSNP:757399152
1769 1769 a, t dbSNP:753984271
1787 1787 c, t dbSNP:778115846
1790 1790 c, t dbSNP:756540757
1793 1793 a, g dbSNP:370394316
1799 1799 c, t dbSNP:768076125
1824 1824 a, g dbSNP:760139789
1825 1825 c, t dbSNP:749905224
1829 1829 a, t dbSNP:764618922
1835 1835 a, g dbSNP:761447530
1838 1838 a, t dbSNP:776147456
1840 1840 -, attt dbSNP:769843457
1843 1843 c, t dbSNP:560190372
1844 1844 a, g dbSNP:146546302
1854 1854 a, g dbSNP:775287768
1855 1855 g, t dbSNP:771959962
1858 1858 -, atg dbSNP:746386623
1858 1858 a, g dbSNP:745775222
1901 1901 g, t dbSNP:769371400
1909 1909 -, a dbSNP:563450219
1910 1910 c, t dbSNP:150413533
1941 1941 a, g dbSNP:3544
1956 1956 -, gat dbSNP:552001777
1958 1958 -, aga dbSNP:112027877
1992 1992 c, t dbSNP:753866564
2031 2031 a, g dbSNP:373528883
2111 2111 c, t dbSNP:766182254
2162 2162 a, g dbSNP:775132795
2185 2185 c, t dbSNP:140595869
2212 2212 a, g dbSNP:765027393
2248 2248 g, t dbSNP:759398749
2284 2284 a, g dbSNP:541432527
2302 2302 a, g dbSNP:544894567
2305 2305 a, g dbSNP:1804733
2333 2333 a, c dbSNP:151333282
2384 2384 -, t dbSNP:376801986
2428 2428 a, g dbSNP:142469019
2460 2460 c, t dbSNP:780234856
2498 2498 a, c, g dbSNP:200779763
2500 2500 -, a dbSNP:397747580
2514 2514 -, a dbSNP:35981443
2551 2551 a, g dbSNP:373423492
2553 2553 c, t dbSNP:573194607
2572 2572 g, t dbSNP:558214787
2585 2585 g, t dbSNP:373013057
2601 2601 c, t dbSNP:539575179
2635 2635 a, t dbSNP:571080195
2661 2661 c, t dbSNP:770374188
2669 2669 g, t dbSNP:11556960
2738 2738 g, t dbSNP:563141520
2786 2786 c, t dbSNP:555954627
2857 2857 c, t dbSNP:537706046
2871 2871 c, t dbSNP:558334072
2901 2901 a, g dbSNP:1049846
2941 2941 a, g dbSNP:12417

Target ORF information:

RefSeq Version NM_002656
Organism Homo sapiens (human)
Definition Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002656

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCTACCC ATTCTCCCCA GAAATCTCAC CAGTGTGCTC ACTGTGAGAA GACGTTCAAC 
CGGAAAGACC ACCTGAAAAA CCACCTCCAG ACCCACGACC CCAACAAAAT GGCCTTTGGG
TGTGAGGAGT GTGGGAAGAA GTACAACACC ATGCTGGGCT ATAAGAGGCA CCTGGCCCTC
CATGCGGCCA GCAGTGGGGA CCTCACCTGT GGGGTCTGTG CCCTGGAGCT AGGGAGCACC
GAGGTGCTAC TGGACCACCT CAAAGCCCAT GCGGAAGAGA AGCCCCCTAG CGGAACCAAG
GAAAAGAAGC ACCAGTGCGA CCACTGTGAA AGATGCTTCT ACACCCGGAA GGATGTGCGA
CGCCACCTGG TGGTCCACAC AGGATGCAAG GACTTCCTGT GCCAGTTCTG TGCCCAGAGA
TTTGGGCGCA AGGATCACCT CACCCGGCAT ACCAAGAAGA CCCACTCACA GGAGCTGATG
AAAGAGAGCT TGCAGACCGG AGACCTTCTG AGCACCTTCC ACACCATCTC GCCTTCATTC
CAACTGAAGG CTGCTGCCTT GCCTCCTTTC CCTTTAGGAG CTTCTGCCCA GAACGGGCTT
GCAAGTAGCT TGCCAGCTGA GGTCCATAGC CTCACCCTCA GTCCCCCAGA ACAAGCCGCC
CAGCCTATGC AGCCGCTGCC AGAGTCCCTG GCCTCCCTCC ACCCCTCGGT ATCCCCTGGC
TCTCCTCCGC CACCCCTTCC CAATCACAAG TACAACACCA CTTCTACCTC ATACTCCCCA
CTTGCAAGCC TGCCCCTCAA AGCAGATACT AAAGGTTTTT GCAATATCAG TTTGTTTGAG
GACTTGCCTC TGCAAGAGCC TCAGTCACCT CAAAAGCTCA ACCCAGGTTT TGATCTGGCT
AAGGGAAATG CTGGTAAAGT AAACCTGCCC AAGGAGCTGC CTGCAGATGC TGTGAACCTA
ACAATACCTG CCTCTCTGGA CCTGTCCCCC CTGTTGGGCT TCTGGCAGCT GCCCCCTCCT
GCTACCCAAA ATACCTTTGG GAATAGCACT CTTGCCCTGG GGCCTGGGGA ATCTTTGCCC
CACAGGTTAA GCTGTCTGGG GCAGCAGCAG CAAGAACCCC CACTTGCCAT GGGCACTGTG
AGCCTGGGCC AGCTCCCCCT GCCCCCCATC CCTCATGTGT TCTCAGCTGG CACTGGCTCT
GCCATCCTGC CTCATTTCCA TCATGCATTC AGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19858
Accession Version NM_006718.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1392bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product zinc finger protein PLAGL1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA446007.1, DA549753.1, DA391124.1, EF100447.1, AJ311395.1 and AI741398.1. On Feb 2, 2007 this sequence version replaced gi:27894292. Summary: This gene encodes a C2H2 zinc finger protein with transactivation and DNA-binding activities. It has been shown to have anti-proliferative properties, and thus thought to function as a tumor suppressor. In addition, overexpression of this gene during fetal development is believed to underlie the rare disorder, transient neonatal diabetes mellitus (TNDM). This gene is imprinted, with preferential expression of the paternal allele in many tissues, however, biallelic expression has been noted in peripheral blood leucocytes. A recent study reports that tissue-specific imprinting results from variable utilization of monoallelic and biallelic promoters. Many transcript variants differing in the 5' UTR and encoding two different isoforms, have been found for this gene. [provided by RefSeq, Oct 2010]. Transcript Variant: This variant (2) differs in the 5' UTR and contains an alternate exon in the CDS compared to variant 1. The resulting isoform (2) is longer and has an extended N-terminus compared to isoform 1. Variants 2, 3, 4, 5, 6, 10, 12, 15, 16, 17, 18, 19 and 20 all encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## imprinted gene :: PMID: 17341487, 10655556 ##RefSeq-Attributes-END## ##Evidence-Data-START## CDS exon combination :: AJ311395.1, BC074814.2 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2162568 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCCACGT TCCCCTGCCA GTTATGTGGC AAGACGTTCC TCACCCTGGA GAAGTTCACG 
ATTCACAATT ATTCCCACTC CAGGGAGCGG CCGTACAAGT GTGTGCAGCC TGACTGTGGC
AAAGCCTTTG TTTCCAGATA TAAATTGATG AGGCATATGG CTACCCATTC TCCCCAGAAA
TCTCACCAGT GTGCTCACTG TGAGAAGACG TTCAACCGGA AAGACCACCT GAAAAACCAC
CTCCAGACCC ACGACCCCAA CAAAATGGCC TTTGGGTGTG AGGAGTGTGG GAAGAAGTAC
AACACCATGC TGGGCTATAA GAGGCACCTG GCCCTCCATG CGGCCAGCAG TGGGGACCTC
ACCTGTGGGG TCTGTGCCCT GGAGCTAGGG AGCACCGAGG TGCTACTGGA CCACCTCAAA
GCCCATGCGG AAGAGAAGCC CCCTAGCGGA ACCAAGGAAA AGAAGCACCA GTGCGACCAC
TGTGAAAGAT GCTTCTACAC CCGGAAGGAT GTGCGACGCC ACCTGGTGGT CCACACAGGA
TGCAAGGACT TCCTGTGCCA GTTCTGTGCC CAGAGATTTG GGCGCAAGGA TCACCTCACC
CGGCATACCA AGAAGACCCA CTCACAGGAG CTGATGAAAG AGAGCTTGCA GACCGGAGAC
CTTCTGAGCA CCTTCCACAC CATCTCGCCT TCATTCCAAC TGAAGGCTGC TGCCTTGCCT
CCTTTCCCTT TAGGAGCTTC TGCCCAGAAC GGGCTTGCAA GTAGCTTGCC AGCTGAGGTC
CATAGCCTCA CCCTCAGTCC CCCAGAACAA GCCGCCCAGC CTATGCAGCC GCTGCCAGAG
TCCCTGGCCT CCCTCCACCC CTCGGTATCC CCTGGCTCTC CTCCGCCACC CCTTCCCAAT
CACAAGTACA ACACCACTTC TACCTCATAC TCCCCACTTG CAAGCCTGCC CCTCAAAGCA
GATACTAAAG GTTTTTGCAA TATCAGTTTG TTTGAGGACT TGCCTCTGCA AGAGCCTCAG
TCACCTCAAA AGCTCAACCC AGGTTTTGAT CTGGCTAAGG GAAATGCTGG TAAAGTAAAC
CTGCCCAAGG AGCTGCCTGC AGATGCTGTG AACCTAACAA TACCTGCCTC TCTGGACCTG
TCCCCCCTGT TGGGCTTCTG GCAGCTGCCC CCTCCTGCTA CCCAAAATAC CTTTGGGAAT
AGCACTCTTG CCCTGGGGCC TGGGGAATCT TTGCCCCACA GGTTAAGCTG TCTGGGGCAG
CAGCAGCAAG AACCCCCACT TGCCATGGGC ACTGTGAGCC TGGGCCAGCT CCCCCTGCCC
CCCATCCCTC ATGTGTTCTC AGCTGGCACT GGCTCTGCCA TCCTGCCTCA TTTCCATCAT
GCATTCAGAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_006709.2
CDS891..2282
Misc Feature(1)876..878(+)
Misc Feature(2)906..968(+)
Misc Feature(3)906..968(+)
Misc Feature(4)921..1139(+)
Misc Feature(5)990..1058(+)
Misc Feature(6)990..1055(+)
Misc Feature(7)1035..1109(+)
Misc Feature(8)1080..1142(+)
Misc Feature(9)1080..1142(+)
Misc Feature(10)1080..1142(+)
Misc Feature(11)1080..1142(+)
Misc Feature(12)1095..1226(+)
Misc Feature(13)1167..1229(+)
Misc Feature(14)1167..1229(+)
Misc Feature(15)1254..1316(+)
Misc Feature(16)1254..1316(+)
Misc Feature(17)1269..1508(+)
Misc Feature(18)1362..1424(+)
Misc Feature(19)1362..1421(+)
Misc Feature(20)1446..1511(+)
Misc Feature(21)1446..1511(+)
Exon (1)1..315
Gene:PLAGL1
Gene Synonym:
Exon (2)316..387
Gene:PLAGL1
Gene Synonym:
Exon (3)388..460
Gene:PLAGL1
Gene Synonym:
Exon (4)461..492
Gene:PLAGL1
Gene Synonym:
Exon (5)493..566
Gene:PLAGL1
Gene Synonym:
Exon (6)567..1042
Gene:PLAGL1
Gene Synonym:
Exon (7)1043..3406
Gene:PLAGL1
Gene Synonym:
Translation
Position Chain Variation Link
98 98 a, g dbSNP:549107424
130 130 a, t dbSNP:188083405
180 180 a, g dbSNP:113189219
264 264 a, g dbSNP:779493627
279 279 a, g dbSNP:67300369
287 287 c, t dbSNP:749380636
368 368 a, t dbSNP:543763464
381 381 c, t dbSNP:573299088
413 413 a, g dbSNP:554648181
421 421 c, t dbSNP:192562088
434 434 a, g dbSNP:368424368
469 469 a, t dbSNP:370220800
526 526 c, t dbSNP:553367951
527 527 a, c, g dbSNP:570633358
539 539 a, g dbSNP:551127170
623 623 c, g dbSNP:2076684
710 710 c, t dbSNP:370977690
714 714 g, t dbSNP:77502692
751 751 c, t dbSNP:138964933
753 753 c, t dbSNP:745769302
754 754 a, g dbSNP:571326035
768 768 c, t dbSNP:549961074
769 769 a, g dbSNP:191543871
779 779 a, g dbSNP:549484273
815 815 c, t dbSNP:531468151
819 819 a, g dbSNP:562539391
822 822 c, t dbSNP:145922363
878 878 a, c dbSNP:750512755
886 886 a, g dbSNP:765374630
898 898 a, c, t dbSNP:529372879
899 899 a, g dbSNP:539999694
900 900 c, t dbSNP:147037984
923 923 a, g dbSNP:776060847
926 926 a, g dbSNP:141506249
935 935 c, t dbSNP:772513575
938 938 c, g dbSNP:147962624
947 947 c, g dbSNP:777429105
949 949 c, t dbSNP:769533076
950 950 a, g dbSNP:201628824
978 978 c, t dbSNP:780760124
979 979 a, g, t dbSNP:201696236
983 983 a, g dbSNP:565249616
984 984 a, t dbSNP:758334227
988 988 a, c dbSNP:750460388
989 989 a, g dbSNP:377168292
1008 1008 c, g dbSNP:761929176
1014 1014 a, c, g dbSNP:371685096
1026 1026 a, g dbSNP:761089335
1027 1027 c, g dbSNP:775732313
1034 1034 a, g dbSNP:367667352
1035 1035 a, c, t dbSNP:186779775
1054 1054 a, c dbSNP:758281178
1088 1088 c, t dbSNP:745781639
1096 1096 -, aga dbSNP:776571919
1100 1100 a, g dbSNP:144954752
1107 1107 c, t dbSNP:757324170
1108 1108 a, g dbSNP:753924230
1142 1142 c, t dbSNP:764320213
1143 1143 a, g, t dbSNP:753068252
1150 1150 a, g dbSNP:768018756
1151 1151 a, c dbSNP:760031131
1153 1153 -, caa dbSNP:766108577
1157 1157 a, g dbSNP:774971840
1165 1165 a, g dbSNP:139967852
1170 1170 a, g dbSNP:767063830
1172 1172 a, g dbSNP:761345864
1187 1187 a, g dbSNP:776006127
1191 1191 a, c dbSNP:768311718
1192 1192 a, c dbSNP:746660705
1214 1214 a, g dbSNP:775255229
1231 1231 a, c, t dbSNP:745728602
1232 1232 a, g dbSNP:778809798
1236 1236 a, c dbSNP:757200008
1240 1240 a, g dbSNP:749325083
1252 1252 c, t dbSNP:201937527
1257 1257 g, t dbSNP:201753929
1259 1259 g, t dbSNP:780620100
1277 1277 a, g dbSNP:368641715
1279 1279 c, g dbSNP:755394360
1281 1281 a, g dbSNP:151100437
1285 1285 c, g dbSNP:766922660
1286 1286 c, t dbSNP:142899386
1290 1290 c, g dbSNP:148432028
1292 1292 g, t dbSNP:763488544
1295 1295 a, c dbSNP:374451635
1296 1296 c, t dbSNP:538240247
1297 1297 c, t dbSNP:771769935
1303 1303 a, t dbSNP:759269789
1311 1311 g, t dbSNP:17847327
1319 1319 a, g dbSNP:17073229
1322 1322 a, g, t dbSNP:777804198
1329 1329 a, c dbSNP:769737512
1333 1333 c, t dbSNP:748313279
1334 1334 a, t dbSNP:781556516
1337 1337 c, t dbSNP:755267953
1338 1338 a, g dbSNP:751926904
1365 1365 a, g dbSNP:780599925
1369 1369 a, g dbSNP:758839660
1393 1393 a, g dbSNP:750874987
1397 1397 a, g dbSNP:765832081
1405 1405 a, g dbSNP:760099365
1415 1415 g, t dbSNP:752208274
1418 1418 a, g dbSNP:267600848
1460 1460 c, t dbSNP:767098050
1475 1475 c, t dbSNP:141784122
1480 1480 a, t dbSNP:1063413
1496 1496 c, t dbSNP:36120645
1508 1508 a, c dbSNP:547586757
1517 1517 a, g dbSNP:762851758
1518 1518 -, gag dbSNP:760478312
1525 1525 c, t dbSNP:773005838
1531 1531 a, g dbSNP:34652819
1533 1533 a, g dbSNP:748123821
1534 1534 c, g dbSNP:781448211
1541 1541 c, g dbSNP:375679973
1544 1544 c, g, t dbSNP:370449852
1545 1545 a, g dbSNP:758678527
1548 1548 a, g dbSNP:565181543
1551 1551 a, c dbSNP:201977118
1555 1555 c, t dbSNP:377135913
1557 1557 a, c dbSNP:752153135
1569 1569 a, g dbSNP:17847328
1572 1572 a, t dbSNP:754521531
1576 1576 c, t dbSNP:751187671
1582 1582 c, t dbSNP:766110989
1590 1590 -, c dbSNP:35411045
1599 1599 a, g dbSNP:560576837
1607 1607 a, g dbSNP:373156842
1609 1609 c, g dbSNP:142705655
1617 1617 c, t dbSNP:761877385
1630 1630 c, g dbSNP:776753534
1631 1631 c, t dbSNP:768835975
1641 1641 a, g dbSNP:372873338
1642 1642 a, g dbSNP:747145351
1659 1659 c, t dbSNP:775851406
1663 1663 c, g dbSNP:369453748
1665 1665 a, g dbSNP:746160419
1673 1673 c, t dbSNP:200072882
1681 1681 c, t dbSNP:757664610
1682 1682 c, t dbSNP:749761427
1698 1698 a, c dbSNP:778298992
1703 1703 c, t dbSNP:149291080
1704 1704 a, c, g dbSNP:765875826
1705 1705 c, t dbSNP:35263016
1715 1715 a, g dbSNP:578145229
1720 1720 c, t dbSNP:377306571
1721 1721 a, g, t dbSNP:776698636
1727 1727 a, g dbSNP:764195119
1728 1728 c, g dbSNP:141235151
1729 1729 a, c dbSNP:775653923
1730 1730 -, ccagag dbSNP:774596210
1730 1730 a, g dbSNP:772136403
1736 1736 a, g dbSNP:62427100
1737 1737 a, g dbSNP:780626452
1742 1742 c, g dbSNP:374462557
1746 1746 a, c dbSNP:774476162
1748 1748 c, t dbSNP:368929894
1752 1752 c, t dbSNP:556887561
1753 1753 c, t dbSNP:749708141
1754 1754 a, g, t dbSNP:201057231
1755 1755 a, g dbSNP:746453740
1759 1759 c, t dbSNP:779649831
1760 1760 c, t dbSNP:757889837
1766 1766 c, t dbSNP:750070946
1774 1774 c, t dbSNP:200573380
1775 1775 a, g dbSNP:17847330
1789 1789 a, t dbSNP:371698312
1801 1801 a, c dbSNP:147054773
1805 1805 c, t dbSNP:200036472
1806 1806 a, g dbSNP:752677729
1807 1807 c, t dbSNP:767601739
1817 1817 a, t dbSNP:759582013
1824 1824 c, t dbSNP:774617149
1825 1825 c, t dbSNP:771206023
1830 1830 a, g, t dbSNP:141627165
1831 1831 a, c dbSNP:770249452
1838 1838 a, c, g dbSNP:147885532
1841 1841 a, c dbSNP:771601130
1842 1842 -, c dbSNP:768840283
1843 1843 a, t dbSNP:745438962
1845 1845 a, c dbSNP:778643791
1866 1866 c, t dbSNP:756984299
1870 1870 a, g dbSNP:374631467
1880 1880 g, t dbSNP:777741438
1887 1887 a, g dbSNP:756012523
1892 1892 a, g dbSNP:752655598
1913 1913 a, t dbSNP:138688804
1925 1925 c, g dbSNP:759653376
1933 1933 a, g dbSNP:751593481
1941 1941 c, g dbSNP:766525788
1943 1943 a, g dbSNP:763180631
1951 1951 a, g dbSNP:773466984
1986 1986 a, c dbSNP:372153249
2002 2002 a, c dbSNP:762144772
2008 2008 a, c dbSNP:777194150
2011 2011 c, t dbSNP:769049757
2012 2012 a, c dbSNP:200257642
2013 2013 c, t dbSNP:774021173
2022 2022 c, g dbSNP:201957325
2024 2024 a, g dbSNP:72546304
2027 2027 c, t dbSNP:145034248
2030 2030 c, g dbSNP:755923312
2032 2032 a, c, g, t dbSNP:754880138
2033 2033 a, c, g dbSNP:145386261
2034 2034 c, g dbSNP:758526896
2036 2036 a, c, g, t dbSNP:150039945
2037 2037 a, c, g dbSNP:764612601
2038 2038 c, t dbSNP:149400255
2039 2039 c, g dbSNP:761118576
2060 2060 a, c, t dbSNP:17847331
2063 2063 c, t dbSNP:748862556
2068 2068 c, t dbSNP:772992931
2072 2072 c, t dbSNP:769332223
2076 2076 a, c dbSNP:747916100
2077 2077 a, c dbSNP:780866537
2079 2079 a, g dbSNP:751709706
2083 2083 c, t dbSNP:754901227
2089 2089 a, g dbSNP:373757589
2091 2091 a, g dbSNP:780028654
2100 2100 a, g dbSNP:758471604
2101 2101 c, t dbSNP:750509016
2108 2108 a, g dbSNP:765442934
2109 2109 -, cctgggg dbSNP:749424025
2109 2109 a, c dbSNP:757445503
2112 2112 a, g dbSNP:754192424
2117 2117 a, c dbSNP:370253986
2121 2121 c, t dbSNP:548577685
2136 2136 a, c dbSNP:776125291
2137 2137 a, g dbSNP:764191640
2143 2143 a, t dbSNP:768204588
2144 2144 a, g dbSNP:762482586
2147 2147 g, t dbSNP:772859501
2149 2149 a, g dbSNP:769435521
2155 2155 a, c dbSNP:374421447
2156 2156 g, t dbSNP:776397265
2158 2158 -, gca dbSNP:775540928
2160 2160 -, c dbSNP:35716705
2164 2164 c, t dbSNP:768326522
2175 2175 a, g dbSNP:746874822
2177 2177 a, c, g dbSNP:527350024
2193 2193 a, g dbSNP:745900352
2213 2213 c, t dbSNP:778890400
2218 2218 a, c dbSNP:36087014
2221 2221 a, g dbSNP:757399152
2231 2231 a, t dbSNP:753984271
2249 2249 c, t dbSNP:778115846
2252 2252 c, t dbSNP:756540757
2255 2255 a, g dbSNP:370394316
2261 2261 c, t dbSNP:768076125
2286 2286 a, g dbSNP:760139789
2287 2287 c, t dbSNP:749905224
2291 2291 a, t dbSNP:764618922
2297 2297 a, g dbSNP:761447530
2300 2300 a, t dbSNP:776147456
2302 2302 -, attt dbSNP:769843457
2305 2305 c, t dbSNP:560190372
2306 2306 a, g dbSNP:146546302
2316 2316 a, g dbSNP:775287768
2317 2317 g, t dbSNP:771959962
2320 2320 -, atg dbSNP:746386623
2320 2320 a, g dbSNP:745775222
2363 2363 g, t dbSNP:769371400
2371 2371 -, a dbSNP:563450219
2372 2372 c, t dbSNP:150413533
2403 2403 a, g dbSNP:3544
2418 2418 -, gat dbSNP:552001777
2420 2420 -, aga dbSNP:112027877
2454 2454 c, t dbSNP:753866564
2493 2493 a, g dbSNP:373528883
2573 2573 c, t dbSNP:766182254
2624 2624 a, g dbSNP:775132795
2647 2647 c, t dbSNP:140595869
2674 2674 a, g dbSNP:765027393
2710 2710 g, t dbSNP:759398749
2746 2746 a, g dbSNP:541432527
2764 2764 a, g dbSNP:544894567
2767 2767 a, g dbSNP:1804733
2795 2795 a, c dbSNP:151333282
2846 2846 -, t dbSNP:376801986
2890 2890 a, g dbSNP:142469019
2922 2922 c, t dbSNP:780234856
2960 2960 a, c, g dbSNP:200779763
2962 2962 -, a dbSNP:397747580
2976 2976 -, a dbSNP:35981443
3013 3013 a, g dbSNP:373423492
3015 3015 c, t dbSNP:573194607
3034 3034 g, t dbSNP:558214787
3047 3047 g, t dbSNP:373013057
3063 3063 c, t dbSNP:539575179
3097 3097 a, t dbSNP:571080195
3123 3123 c, t dbSNP:770374188
3131 3131 g, t dbSNP:11556960
3200 3200 g, t dbSNP:563141520
3248 3248 c, t dbSNP:555954627
3319 3319 c, t dbSNP:537706046
3333 3333 c, t dbSNP:558334072
3363 3363 a, g dbSNP:1049846
3403 3403 a, g dbSNP:12417

Target ORF information:

RefSeq Version NM_006718
Organism Homo sapiens (human)
Definition Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006718

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCCACGT TCCCCTGCCA GTTATGTGGC AAGACGTTCC TCACCCTGGA GAAGTTCACG 
ATTCACAATT ATTCCCACTC CAGGGAGCGG CCGTACAAGT GTGTGCAGCC TGACTGTGGC
AAAGCCTTTG TTTCCAGATA TAAATTGATG AGGCATATGG CTACCCATTC TCCCCAGAAA
TCTCACCAGT GTGCTCACTG TGAGAAGACG TTCAACCGGA AAGACCACCT GAAAAACCAC
CTCCAGACCC ACGACCCCAA CAAAATGGCC TTTGGGTGTG AGGAGTGTGG GAAGAAGTAC
AACACCATGC TGGGCTATAA GAGGCACCTG GCCCTCCATG CGGCCAGCAG TGGGGACCTC
ACCTGTGGGG TCTGTGCCCT GGAGCTAGGG AGCACCGAGG TGCTACTGGA CCACCTCAAA
GCCCATGCGG AAGAGAAGCC CCCTAGCGGA ACCAAGGAAA AGAAGCACCA GTGCGACCAC
TGTGAAAGAT GCTTCTACAC CCGGAAGGAT GTGCGACGCC ACCTGGTGGT CCACACAGGA
TGCAAGGACT TCCTGTGCCA GTTCTGTGCC CAGAGATTTG GGCGCAAGGA TCACCTCACC
CGGCATACCA AGAAGACCCA CTCACAGGAG CTGATGAAAG AGAGCTTGCA GACCGGAGAC
CTTCTGAGCA CCTTCCACAC CATCTCGCCT TCATTCCAAC TGAAGGCTGC TGCCTTGCCT
CCTTTCCCTT TAGGAGCTTC TGCCCAGAAC GGGCTTGCAA GTAGCTTGCC AGCTGAGGTC
CATAGCCTCA CCCTCAGTCC CCCAGAACAA GCCGCCCAGC CTATGCAGCC GCTGCCAGAG
TCCCTGGCCT CCCTCCACCC CTCGGTATCC CCTGGCTCTC CTCCGCCACC CCTTCCCAAT
CACAAGTACA ACACCACTTC TACCTCATAC TCCCCACTTG CAAGCCTGCC CCTCAAAGCA
GATACTAAAG GTTTTTGCAA TATCAGTTTG TTTGAGGACT TGCCTCTGCA AGAGCCTCAG
TCACCTCAAA AGCTCAACCC AGGTTTTGAT CTGGCTAAGG GAAATGCTGG TAAAGTAAAC
CTGCCCAAGG AGCTGCCTGC AGATGCTGTG AACCTAACAA TACCTGCCTC TCTGGACCTG
TCCCCCCTGT TGGGCTTCTG GCAGCTGCCC CCTCCTGCTA CCCAAAATAC CTTTGGGAAT
AGCACTCTTG CCCTGGGGCC TGGGGAATCT TTGCCCCACA GGTTAAGCTG TCTGGGGCAG
CAGCAGCAAG AACCCCCACT TGCCATGGGC ACTGTGAGCC TGGGCCAGCT CCCCCTGCCC
CCCATCCCTC ATGTGTTCTC AGCTGGCACT GGCTCTGCCA TCCTGCCTCA TTTCCATCAT
GCATTCAGAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19858
Accession Version NM_001080951.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1392bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product zinc finger protein PLAGL1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA991276.1, EF100448.1, AJ311395.1 and AI741398.1. This sequence is a reference standard in the RefSeqGene project. Summary: This gene encodes a C2H2 zinc finger protein with transactivation and DNA-binding activities. It has been shown to have anti-proliferative properties, and thus thought to function as a tumor suppressor. In addition, overexpression of this gene during fetal development is believed to underlie the rare disorder, transient neonatal diabetes mellitus (TNDM). This gene is imprinted, with preferential expression of the paternal allele in many tissues, however, biallelic expression has been noted in peripheral blood leucocytes. A recent study reports that tissue-specific imprinting results from variable utilization of monoallelic and biallelic promoters. Many transcript variants differing in the 5' UTR and encoding two different isoforms, have been found for this gene. [provided by RefSeq, Oct 2010]. Transcript Variant: This variant (3) differs in the 5' UTR and contains an alternate exon in the CDS compared to variant 1. The resulting isoform (2) is longer and has an extended N-terminus compared to isoform 1. Variants 2, 3, 4, 5, 6, 10, 12, 15, 16, 17, 18, 19 and 20 all encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## imprinted gene :: PMID: 17341487, 10655556 ##RefSeq-Attributes-END## ##Evidence-Data-START## CDS exon combination :: AJ311395.1, BC074814.2 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2162568 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCCACGT TCCCCTGCCA GTTATGTGGC AAGACGTTCC TCACCCTGGA GAAGTTCACG 
ATTCACAATT ATTCCCACTC CAGGGAGCGG CCGTACAAGT GTGTGCAGCC TGACTGTGGC
AAAGCCTTTG TTTCCAGATA TAAATTGATG AGGCATATGG CTACCCATTC TCCCCAGAAA
TCTCACCAGT GTGCTCACTG TGAGAAGACG TTCAACCGGA AAGACCACCT GAAAAACCAC
CTCCAGACCC ACGACCCCAA CAAAATGGCC TTTGGGTGTG AGGAGTGTGG GAAGAAGTAC
AACACCATGC TGGGCTATAA GAGGCACCTG GCCCTCCATG CGGCCAGCAG TGGGGACCTC
ACCTGTGGGG TCTGTGCCCT GGAGCTAGGG AGCACCGAGG TGCTACTGGA CCACCTCAAA
GCCCATGCGG AAGAGAAGCC CCCTAGCGGA ACCAAGGAAA AGAAGCACCA GTGCGACCAC
TGTGAAAGAT GCTTCTACAC CCGGAAGGAT GTGCGACGCC ACCTGGTGGT CCACACAGGA
TGCAAGGACT TCCTGTGCCA GTTCTGTGCC CAGAGATTTG GGCGCAAGGA TCACCTCACC
CGGCATACCA AGAAGACCCA CTCACAGGAG CTGATGAAAG AGAGCTTGCA GACCGGAGAC
CTTCTGAGCA CCTTCCACAC CATCTCGCCT TCATTCCAAC TGAAGGCTGC TGCCTTGCCT
CCTTTCCCTT TAGGAGCTTC TGCCCAGAAC GGGCTTGCAA GTAGCTTGCC AGCTGAGGTC
CATAGCCTCA CCCTCAGTCC CCCAGAACAA GCCGCCCAGC CTATGCAGCC GCTGCCAGAG
TCCCTGGCCT CCCTCCACCC CTCGGTATCC CCTGGCTCTC CTCCGCCACC CCTTCCCAAT
CACAAGTACA ACACCACTTC TACCTCATAC TCCCCACTTG CAAGCCTGCC CCTCAAAGCA
GATACTAAAG GTTTTTGCAA TATCAGTTTG TTTGAGGACT TGCCTCTGCA AGAGCCTCAG
TCACCTCAAA AGCTCAACCC AGGTTTTGAT CTGGCTAAGG GAAATGCTGG TAAAGTAAAC
CTGCCCAAGG AGCTGCCTGC AGATGCTGTG AACCTAACAA TACCTGCCTC TCTGGACCTG
TCCCCCCTGT TGGGCTTCTG GCAGCTGCCC CCTCCTGCTA CCCAAAATAC CTTTGGGAAT
AGCACTCTTG CCCTGGGGCC TGGGGAATCT TTGCCCCACA GGTTAAGCTG TCTGGGGCAG
CAGCAGCAAG AACCCCCACT TGCCATGGGC ACTGTGAGCC TGGGCCAGCT CCCCCTGCCC
CCCATCCCTC ATGTGTTCTC AGCTGGCACT GGCTCTGCCA TCCTGCCTCA TTTCCATCAT
GCATTCAGAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001074420.1
CDS764..2155
Misc Feature(1)749..751(+)
Misc Feature(2)779..841(+)
Misc Feature(3)779..841(+)
Misc Feature(4)794..1012(+)
Misc Feature(5)863..931(+)
Misc Feature(6)863..928(+)
Misc Feature(7)908..982(+)
Misc Feature(8)953..1015(+)
Misc Feature(9)953..1015(+)
Misc Feature(10)953..1015(+)
Misc Feature(11)953..1015(+)
Misc Feature(12)968..1099(+)
Misc Feature(13)1040..1102(+)
Misc Feature(14)1040..1102(+)
Misc Feature(15)1127..1189(+)
Misc Feature(16)1127..1189(+)
Misc Feature(17)1142..1381(+)
Misc Feature(18)1235..1297(+)
Misc Feature(19)1235..1294(+)
Misc Feature(20)1319..1384(+)
Misc Feature(21)1319..1384(+)
Exon (1)1..148
Gene:PLAGL1
Gene Synonym:
Exon (2)149..188
Gene:PLAGL1
Gene Synonym:
Exon (3)189..260
Gene:PLAGL1
Gene Synonym:
Exon (4)261..333
Gene:PLAGL1
Gene Synonym:
Exon (5)334..365
Gene:PLAGL1
Gene Synonym:
Exon (6)366..439
Gene:PLAGL1
Gene Synonym:
Exon (7)440..915
Gene:PLAGL1
Gene Synonym:
Exon (8)916..3279
Gene:PLAGL1
Gene Synonym:
Translation
Position Chain Variation Link
35 35 a, c dbSNP:542102544
45 45 c, g dbSNP:13194924
59 59 c, t dbSNP:556207078
112 112 a, c dbSNP:3734225
120 120 a, g dbSNP:573789329
166 166 a, c dbSNP:182593182
241 241 a, t dbSNP:543763464
254 254 c, t dbSNP:573299088
286 286 a, g dbSNP:554648181
294 294 c, t dbSNP:192562088
307 307 a, g dbSNP:368424368
342 342 a, t dbSNP:370220800
399 399 c, t dbSNP:553367951
400 400 a, c, g dbSNP:570633358
412 412 a, g dbSNP:551127170
496 496 c, g dbSNP:2076684
583 583 c, t dbSNP:370977690
587 587 g, t dbSNP:77502692
624 624 c, t dbSNP:138964933
626 626 c, t dbSNP:745769302
627 627 a, g dbSNP:571326035
641 641 c, t dbSNP:549961074
642 642 a, g dbSNP:191543871
652 652 a, g dbSNP:549484273
688 688 c, t dbSNP:531468151
692 692 a, g dbSNP:562539391
695 695 c, t dbSNP:145922363
751 751 a, c dbSNP:750512755
759 759 a, g dbSNP:765374630
771 771 a, c, t dbSNP:529372879
772 772 a, g dbSNP:539999694
773 773 c, t dbSNP:147037984
796 796 a, g dbSNP:776060847
799 799 a, g dbSNP:141506249
808 808 c, t dbSNP:772513575
811 811 c, g dbSNP:147962624
820 820 c, g dbSNP:777429105
822 822 c, t dbSNP:769533076
823 823 a, g dbSNP:201628824
851 851 c, t dbSNP:780760124
852 852 a, g, t dbSNP:201696236
856 856 a, g dbSNP:565249616
857 857 a, t dbSNP:758334227
861 861 a, c dbSNP:750460388
862 862 a, g dbSNP:377168292
881 881 c, g dbSNP:761929176
887 887 a, c, g dbSNP:371685096
899 899 a, g dbSNP:761089335
900 900 c, g dbSNP:775732313
907 907 a, g dbSNP:367667352
908 908 a, c, t dbSNP:186779775
927 927 a, c dbSNP:758281178
961 961 c, t dbSNP:745781639
969 969 -, aga dbSNP:776571919
973 973 a, g dbSNP:144954752
980 980 c, t dbSNP:757324170
981 981 a, g dbSNP:753924230
1015 1015 c, t dbSNP:764320213
1016 1016 a, g, t dbSNP:753068252
1023 1023 a, g dbSNP:768018756
1024 1024 a, c dbSNP:760031131
1026 1026 -, caa dbSNP:766108577
1030 1030 a, g dbSNP:774971840
1038 1038 a, g dbSNP:139967852
1043 1043 a, g dbSNP:767063830
1045 1045 a, g dbSNP:761345864
1060 1060 a, g dbSNP:776006127
1064 1064 a, c dbSNP:768311718
1065 1065 a, c dbSNP:746660705
1087 1087 a, g dbSNP:775255229
1104 1104 a, c, t dbSNP:745728602
1105 1105 a, g dbSNP:778809798
1109 1109 a, c dbSNP:757200008
1113 1113 a, g dbSNP:749325083
1125 1125 c, t dbSNP:201937527
1130 1130 g, t dbSNP:201753929
1132 1132 g, t dbSNP:780620100
1150 1150 a, g dbSNP:368641715
1152 1152 c, g dbSNP:755394360
1154 1154 a, g dbSNP:151100437
1158 1158 c, g dbSNP:766922660
1159 1159 c, t dbSNP:142899386
1163 1163 c, g dbSNP:148432028
1165 1165 g, t dbSNP:763488544
1168 1168 a, c dbSNP:374451635
1169 1169 c, t dbSNP:538240247
1170 1170 c, t dbSNP:771769935
1176 1176 a, t dbSNP:759269789
1184 1184 g, t dbSNP:17847327
1192 1192 a, g dbSNP:17073229
1195 1195 a, g, t dbSNP:777804198
1202 1202 a, c dbSNP:769737512
1206 1206 c, t dbSNP:748313279
1207 1207 a, t dbSNP:781556516
1210 1210 c, t dbSNP:755267953
1211 1211 a, g dbSNP:751926904
1238 1238 a, g dbSNP:780599925
1242 1242 a, g dbSNP:758839660
1266 1266 a, g dbSNP:750874987
1270 1270 a, g dbSNP:765832081
1278 1278 a, g dbSNP:760099365
1288 1288 g, t dbSNP:752208274
1291 1291 a, g dbSNP:267600848
1333 1333 c, t dbSNP:767098050
1348 1348 c, t dbSNP:141784122
1353 1353 a, t dbSNP:1063413
1369 1369 c, t dbSNP:36120645
1381 1381 a, c dbSNP:547586757
1390 1390 a, g dbSNP:762851758
1391 1391 -, gag dbSNP:760478312
1398 1398 c, t dbSNP:773005838
1404 1404 a, g dbSNP:34652819
1406 1406 a, g dbSNP:748123821
1407 1407 c, g dbSNP:781448211
1414 1414 c, g dbSNP:375679973
1417 1417 c, g, t dbSNP:370449852
1418 1418 a, g dbSNP:758678527
1421 1421 a, g dbSNP:565181543
1424 1424 a, c dbSNP:201977118
1428 1428 c, t dbSNP:377135913
1430 1430 a, c dbSNP:752153135
1442 1442 a, g dbSNP:17847328
1445 1445 a, t dbSNP:754521531
1449 1449 c, t dbSNP:751187671
1455 1455 c, t dbSNP:766110989
1463 1463 -, c dbSNP:35411045
1472 1472 a, g dbSNP:560576837
1480 1480 a, g dbSNP:373156842
1482 1482 c, g dbSNP:142705655
1490 1490 c, t dbSNP:761877385
1503 1503 c, g dbSNP:776753534
1504 1504 c, t dbSNP:768835975
1514 1514 a, g dbSNP:372873338
1515 1515 a, g dbSNP:747145351
1532 1532 c, t dbSNP:775851406
1536 1536 c, g dbSNP:369453748
1538 1538 a, g dbSNP:746160419
1546 1546 c, t dbSNP:200072882
1554 1554 c, t dbSNP:757664610
1555 1555 c, t dbSNP:749761427
1571 1571 a, c dbSNP:778298992
1576 1576 c, t dbSNP:149291080
1577 1577 a, c, g dbSNP:765875826
1578 1578 c, t dbSNP:35263016
1588 1588 a, g dbSNP:578145229
1593 1593 c, t dbSNP:377306571
1594 1594 a, g, t dbSNP:776698636
1600 1600 a, g dbSNP:764195119
1601 1601 c, g dbSNP:141235151
1602 1602 a, c dbSNP:775653923
1603 1603 -, ccagag dbSNP:774596210
1603 1603 a, g dbSNP:772136403
1609 1609 a, g dbSNP:62427100
1610 1610 a, g dbSNP:780626452
1615 1615 c, g dbSNP:374462557
1619 1619 a, c dbSNP:774476162
1621 1621 c, t dbSNP:368929894
1625 1625 c, t dbSNP:556887561
1626 1626 c, t dbSNP:749708141
1627 1627 a, g, t dbSNP:201057231
1628 1628 a, g dbSNP:746453740
1632 1632 c, t dbSNP:779649831
1633 1633 c, t dbSNP:757889837
1639 1639 c, t dbSNP:750070946
1647 1647 c, t dbSNP:200573380
1648 1648 a, g dbSNP:17847330
1662 1662 a, t dbSNP:371698312
1674 1674 a, c dbSNP:147054773
1678 1678 c, t dbSNP:200036472
1679 1679 a, g dbSNP:752677729
1680 1680 c, t dbSNP:767601739
1690 1690 a, t dbSNP:759582013
1697 1697 c, t dbSNP:774617149
1698 1698 c, t dbSNP:771206023
1703 1703 a, g, t dbSNP:141627165
1704 1704 a, c dbSNP:770249452
1711 1711 a, c, g dbSNP:147885532
1714 1714 a, c dbSNP:771601130
1715 1715 -, c dbSNP:768840283
1716 1716 a, t dbSNP:745438962
1718 1718 a, c dbSNP:778643791
1739 1739 c, t dbSNP:756984299
1743 1743 a, g dbSNP:374631467
1753 1753 g, t dbSNP:777741438
1760 1760 a, g dbSNP:756012523
1765 1765 a, g dbSNP:752655598
1786 1786 a, t dbSNP:138688804
1798 1798 c, g dbSNP:759653376
1806 1806 a, g dbSNP:751593481
1814 1814 c, g dbSNP:766525788
1816 1816 a, g dbSNP:763180631
1824 1824 a, g dbSNP:773466984
1859 1859 a, c dbSNP:372153249
1875 1875 a, c dbSNP:762144772
1881 1881 a, c dbSNP:777194150
1884 1884 c, t dbSNP:769049757
1885 1885 a, c dbSNP:200257642
1886 1886 c, t dbSNP:774021173
1895 1895 c, g dbSNP:201957325
1897 1897 a, g dbSNP:72546304
1900 1900 c, t dbSNP:145034248
1903 1903 c, g dbSNP:755923312
1905 1905 a, c, g, t dbSNP:754880138
1906 1906 a, c, g dbSNP:145386261
1907 1907 c, g dbSNP:758526896
1909 1909 a, c, g, t dbSNP:150039945
1910 1910 a, c, g dbSNP:764612601
1911 1911 c, t dbSNP:149400255
1912 1912 c, g dbSNP:761118576
1933 1933 a, c, t dbSNP:17847331
1936 1936 c, t dbSNP:748862556
1941 1941 c, t dbSNP:772992931
1945 1945 c, t dbSNP:769332223
1949 1949 a, c dbSNP:747916100
1950 1950 a, c dbSNP:780866537
1952 1952 a, g dbSNP:751709706
1956 1956 c, t dbSNP:754901227
1962 1962 a, g dbSNP:373757589
1964 1964 a, g dbSNP:780028654
1973 1973 a, g dbSNP:758471604
1974 1974 c, t dbSNP:750509016
1981 1981 a, g dbSNP:765442934
1982 1982 -, cctgggg dbSNP:749424025
1982 1982 a, c dbSNP:757445503
1985 1985 a, g dbSNP:754192424
1990 1990 a, c dbSNP:370253986
1994 1994 c, t dbSNP:548577685
2009 2009 a, c dbSNP:776125291
2010 2010 a, g dbSNP:764191640
2016 2016 a, t dbSNP:768204588
2017 2017 a, g dbSNP:762482586
2020 2020 g, t dbSNP:772859501
2022 2022 a, g dbSNP:769435521
2028 2028 a, c dbSNP:374421447
2029 2029 g, t dbSNP:776397265
2031 2031 -, gca dbSNP:775540928
2033 2033 -, c dbSNP:35716705
2037 2037 c, t dbSNP:768326522
2048 2048 a, g dbSNP:746874822
2050 2050 a, c, g dbSNP:527350024
2066 2066 a, g dbSNP:745900352
2086 2086 c, t dbSNP:778890400
2091 2091 a, c dbSNP:36087014
2094 2094 a, g dbSNP:757399152
2104 2104 a, t dbSNP:753984271
2122 2122 c, t dbSNP:778115846
2125 2125 c, t dbSNP:756540757
2128 2128 a, g dbSNP:370394316
2134 2134 c, t dbSNP:768076125
2159 2159 a, g dbSNP:760139789
2160 2160 c, t dbSNP:749905224
2164 2164 a, t dbSNP:764618922
2170 2170 a, g dbSNP:761447530
2173 2173 a, t dbSNP:776147456
2175 2175 -, attt dbSNP:769843457
2178 2178 c, t dbSNP:560190372
2179 2179 a, g dbSNP:146546302
2189 2189 a, g dbSNP:775287768
2190 2190 g, t dbSNP:771959962
2193 2193 -, atg dbSNP:746386623
2193 2193 a, g dbSNP:745775222
2236 2236 g, t dbSNP:769371400
2244 2244 -, a dbSNP:563450219
2245 2245 c, t dbSNP:150413533
2276 2276 a, g dbSNP:3544
2291 2291 -, gat dbSNP:552001777
2293 2293 -, aga dbSNP:112027877
2327 2327 c, t dbSNP:753866564
2366 2366 a, g dbSNP:373528883
2446 2446 c, t dbSNP:766182254
2497 2497 a, g dbSNP:775132795
2520 2520 c, t dbSNP:140595869
2547 2547 a, g dbSNP:765027393
2583 2583 g, t dbSNP:759398749
2619 2619 a, g dbSNP:541432527
2637 2637 a, g dbSNP:544894567
2640 2640 a, g dbSNP:1804733
2668 2668 a, c dbSNP:151333282
2719 2719 -, t dbSNP:376801986
2763 2763 a, g dbSNP:142469019
2795 2795 c, t dbSNP:780234856
2833 2833 a, c, g dbSNP:200779763
2835 2835 -, a dbSNP:397747580
2849 2849 -, a dbSNP:35981443
2886 2886 a, g dbSNP:373423492
2888 2888 c, t dbSNP:573194607
2907 2907 g, t dbSNP:558214787
2920 2920 g, t dbSNP:373013057
2936 2936 c, t dbSNP:539575179
2970 2970 a, t dbSNP:571080195
2996 2996 c, t dbSNP:770374188
3004 3004 g, t dbSNP:11556960
3073 3073 g, t dbSNP:563141520
3121 3121 c, t dbSNP:555954627
3192 3192 c, t dbSNP:537706046
3206 3206 c, t dbSNP:558334072
3236 3236 a, g dbSNP:1049846
3276 3276 a, g dbSNP:12417

Target ORF information:

RefSeq Version NM_001080951
Organism Homo sapiens (human)
Definition Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001080951

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCCACGT TCCCCTGCCA GTTATGTGGC AAGACGTTCC TCACCCTGGA GAAGTTCACG 
ATTCACAATT ATTCCCACTC CAGGGAGCGG CCGTACAAGT GTGTGCAGCC TGACTGTGGC
AAAGCCTTTG TTTCCAGATA TAAATTGATG AGGCATATGG CTACCCATTC TCCCCAGAAA
TCTCACCAGT GTGCTCACTG TGAGAAGACG TTCAACCGGA AAGACCACCT GAAAAACCAC
CTCCAGACCC ACGACCCCAA CAAAATGGCC TTTGGGTGTG AGGAGTGTGG GAAGAAGTAC
AACACCATGC TGGGCTATAA GAGGCACCTG GCCCTCCATG CGGCCAGCAG TGGGGACCTC
ACCTGTGGGG TCTGTGCCCT GGAGCTAGGG AGCACCGAGG TGCTACTGGA CCACCTCAAA
GCCCATGCGG AAGAGAAGCC CCCTAGCGGA ACCAAGGAAA AGAAGCACCA GTGCGACCAC
TGTGAAAGAT GCTTCTACAC CCGGAAGGAT GTGCGACGCC ACCTGGTGGT CCACACAGGA
TGCAAGGACT TCCTGTGCCA GTTCTGTGCC CAGAGATTTG GGCGCAAGGA TCACCTCACC
CGGCATACCA AGAAGACCCA CTCACAGGAG CTGATGAAAG AGAGCTTGCA GACCGGAGAC
CTTCTGAGCA CCTTCCACAC CATCTCGCCT TCATTCCAAC TGAAGGCTGC TGCCTTGCCT
CCTTTCCCTT TAGGAGCTTC TGCCCAGAAC GGGCTTGCAA GTAGCTTGCC AGCTGAGGTC
CATAGCCTCA CCCTCAGTCC CCCAGAACAA GCCGCCCAGC CTATGCAGCC GCTGCCAGAG
TCCCTGGCCT CCCTCCACCC CTCGGTATCC CCTGGCTCTC CTCCGCCACC CCTTCCCAAT
CACAAGTACA ACACCACTTC TACCTCATAC TCCCCACTTG CAAGCCTGCC CCTCAAAGCA
GATACTAAAG GTTTTTGCAA TATCAGTTTG TTTGAGGACT TGCCTCTGCA AGAGCCTCAG
TCACCTCAAA AGCTCAACCC AGGTTTTGAT CTGGCTAAGG GAAATGCTGG TAAAGTAAAC
CTGCCCAAGG AGCTGCCTGC AGATGCTGTG AACCTAACAA TACCTGCCTC TCTGGACCTG
TCCCCCCTGT TGGGCTTCTG GCAGCTGCCC CCTCCTGCTA CCCAAAATAC CTTTGGGAAT
AGCACTCTTG CCCTGGGGCC TGGGGAATCT TTGCCCCACA GGTTAAGCTG TCTGGGGCAG
CAGCAGCAAG AACCCCCACT TGCCATGGGC ACTGTGAGCC TGGGCCAGCT CCCCCTGCCC
CCCATCCCTC ATGTGTTCTC AGCTGGCACT GGCTCTGCCA TCCTGCCTCA TTTCCATCAT
GCATTCAGAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19858
Accession Version NM_001080952.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1392bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product zinc finger protein PLAGL1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA446007.1, DA549753.1, EF100452.1, EF100448.1, AJ311395.1 and AI741398.1. Summary: This gene encodes a C2H2 zinc finger protein with transactivation and DNA-binding activities. It has been shown to have anti-proliferative properties, and thus thought to function as a tumor suppressor. In addition, overexpression of this gene during fetal development is believed to underlie the rare disorder, transient neonatal diabetes mellitus (TNDM). This gene is imprinted, with preferential expression of the paternal allele in many tissues, however, biallelic expression has been noted in peripheral blood leucocytes. A recent study reports that tissue-specific imprinting results from variable utilization of monoallelic and biallelic promoters. Many transcript variants differing in the 5' UTR and encoding two different isoforms, have been found for this gene. [provided by RefSeq, Oct 2010]. Transcript Variant: This variant (4) differs in the 5' UTR and contains an alternate exon in the CDS compared to variant 1. The resulting isoform (2) is longer and has an extended N-terminus compared to isoform 1. Variants 2, 3, 4, 5, 6, 10, 12, 15, 16, 17, 18, 19 and 20 all encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## imprinted gene :: PMID: 17341487, 10655556 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: EF100452.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162568 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCCACGT TCCCCTGCCA GTTATGTGGC AAGACGTTCC TCACCCTGGA GAAGTTCACG 
ATTCACAATT ATTCCCACTC CAGGGAGCGG CCGTACAAGT GTGTGCAGCC TGACTGTGGC
AAAGCCTTTG TTTCCAGATA TAAATTGATG AGGCATATGG CTACCCATTC TCCCCAGAAA
TCTCACCAGT GTGCTCACTG TGAGAAGACG TTCAACCGGA AAGACCACCT GAAAAACCAC
CTCCAGACCC ACGACCCCAA CAAAATGGCC TTTGGGTGTG AGGAGTGTGG GAAGAAGTAC
AACACCATGC TGGGCTATAA GAGGCACCTG GCCCTCCATG CGGCCAGCAG TGGGGACCTC
ACCTGTGGGG TCTGTGCCCT GGAGCTAGGG AGCACCGAGG TGCTACTGGA CCACCTCAAA
GCCCATGCGG AAGAGAAGCC CCCTAGCGGA ACCAAGGAAA AGAAGCACCA GTGCGACCAC
TGTGAAAGAT GCTTCTACAC CCGGAAGGAT GTGCGACGCC ACCTGGTGGT CCACACAGGA
TGCAAGGACT TCCTGTGCCA GTTCTGTGCC CAGAGATTTG GGCGCAAGGA TCACCTCACC
CGGCATACCA AGAAGACCCA CTCACAGGAG CTGATGAAAG AGAGCTTGCA GACCGGAGAC
CTTCTGAGCA CCTTCCACAC CATCTCGCCT TCATTCCAAC TGAAGGCTGC TGCCTTGCCT
CCTTTCCCTT TAGGAGCTTC TGCCCAGAAC GGGCTTGCAA GTAGCTTGCC AGCTGAGGTC
CATAGCCTCA CCCTCAGTCC CCCAGAACAA GCCGCCCAGC CTATGCAGCC GCTGCCAGAG
TCCCTGGCCT CCCTCCACCC CTCGGTATCC CCTGGCTCTC CTCCGCCACC CCTTCCCAAT
CACAAGTACA ACACCACTTC TACCTCATAC TCCCCACTTG CAAGCCTGCC CCTCAAAGCA
GATACTAAAG GTTTTTGCAA TATCAGTTTG TTTGAGGACT TGCCTCTGCA AGAGCCTCAG
TCACCTCAAA AGCTCAACCC AGGTTTTGAT CTGGCTAAGG GAAATGCTGG TAAAGTAAAC
CTGCCCAAGG AGCTGCCTGC AGATGCTGTG AACCTAACAA TACCTGCCTC TCTGGACCTG
TCCCCCCTGT TGGGCTTCTG GCAGCTGCCC CCTCCTGCTA CCCAAAATAC CTTTGGGAAT
AGCACTCTTG CCCTGGGGCC TGGGGAATCT TTGCCCCACA GGTTAAGCTG TCTGGGGCAG
CAGCAGCAAG AACCCCCACT TGCCATGGGC ACTGTGAGCC TGGGCCAGCT CCCCCTGCCC
CCCATCCCTC ATGTGTTCTC AGCTGGCACT GGCTCTGCCA TCCTGCCTCA TTTCCATCAT
GCATTCAGAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001074421.1
CDS905..2296
Misc Feature(1)890..892(+)
Misc Feature(2)920..982(+)
Misc Feature(3)920..982(+)
Misc Feature(4)935..1153(+)
Misc Feature(5)1004..1072(+)
Misc Feature(6)1004..1069(+)
Misc Feature(7)1049..1123(+)
Misc Feature(8)1094..1156(+)
Misc Feature(9)1094..1156(+)
Misc Feature(10)1094..1156(+)
Misc Feature(11)1094..1156(+)
Misc Feature(12)1109..1240(+)
Misc Feature(13)1181..1243(+)
Misc Feature(14)1181..1243(+)
Misc Feature(15)1268..1330(+)
Misc Feature(16)1268..1330(+)
Misc Feature(17)1283..1522(+)
Misc Feature(18)1376..1438(+)
Misc Feature(19)1376..1435(+)
Misc Feature(20)1460..1525(+)
Misc Feature(21)1460..1525(+)
Exon (1)1..315
Gene:PLAGL1
Gene Synonym:
Exon (2)316..387
Gene:PLAGL1
Gene Synonym:
Exon (3)388..460
Gene:PLAGL1
Gene Synonym:
Exon (4)461..492
Gene:PLAGL1
Gene Synonym:
Exon (5)493..580
Gene:PLAGL1
Gene Synonym:
Exon (6)581..1056
Gene:PLAGL1
Gene Synonym:
Exon (7)1057..3420
Gene:PLAGL1
Gene Synonym:
Translation
Position Chain Variation Link
98 98 a, g dbSNP:549107424
130 130 a, t dbSNP:188083405
180 180 a, g dbSNP:113189219
264 264 a, g dbSNP:779493627
279 279 a, g dbSNP:67300369
287 287 c, t dbSNP:749380636
368 368 a, t dbSNP:543763464
381 381 c, t dbSNP:573299088
413 413 a, g dbSNP:554648181
421 421 c, t dbSNP:192562088
434 434 a, g dbSNP:368424368
469 469 a, t dbSNP:370220800
540 540 c, t dbSNP:553367951
541 541 a, c, g dbSNP:570633358
553 553 a, g dbSNP:551127170
637 637 c, g dbSNP:2076684
724 724 c, t dbSNP:370977690
728 728 g, t dbSNP:77502692
765 765 c, t dbSNP:138964933
767 767 c, t dbSNP:745769302
768 768 a, g dbSNP:571326035
782 782 c, t dbSNP:549961074
783 783 a, g dbSNP:191543871
793 793 a, g dbSNP:549484273
829 829 c, t dbSNP:531468151
833 833 a, g dbSNP:562539391
836 836 c, t dbSNP:145922363
892 892 a, c dbSNP:750512755
900 900 a, g dbSNP:765374630
912 912 a, c, t dbSNP:529372879
913 913 a, g dbSNP:539999694
914 914 c, t dbSNP:147037984
937 937 a, g dbSNP:776060847
940 940 a, g dbSNP:141506249
949 949 c, t dbSNP:772513575
952 952 c, g dbSNP:147962624
961 961 c, g dbSNP:777429105
963 963 c, t dbSNP:769533076
964 964 a, g dbSNP:201628824
992 992 c, t dbSNP:780760124
993 993 a, g, t dbSNP:201696236
997 997 a, g dbSNP:565249616
998 998 a, t dbSNP:758334227
1002 1002 a, c dbSNP:750460388
1003 1003 a, g dbSNP:377168292
1022 1022 c, g dbSNP:761929176
1028 1028 a, c, g dbSNP:371685096
1040 1040 a, g dbSNP:761089335
1041 1041 c, g dbSNP:775732313
1048 1048 a, g dbSNP:367667352
1049 1049 a, c, t dbSNP:186779775
1068 1068 a, c dbSNP:758281178
1102 1102 c, t dbSNP:745781639
1110 1110 -, aga dbSNP:776571919
1114 1114 a, g dbSNP:144954752
1121 1121 c, t dbSNP:757324170
1122 1122 a, g dbSNP:753924230
1156 1156 c, t dbSNP:764320213
1157 1157 a, g, t dbSNP:753068252
1164 1164 a, g dbSNP:768018756
1165 1165 a, c dbSNP:760031131
1167 1167 -, caa dbSNP:766108577
1171 1171 a, g dbSNP:774971840
1179 1179 a, g dbSNP:139967852
1184 1184 a, g dbSNP:767063830
1186 1186 a, g dbSNP:761345864
1201 1201 a, g dbSNP:776006127
1205 1205 a, c dbSNP:768311718
1206 1206 a, c dbSNP:746660705
1228 1228 a, g dbSNP:775255229
1245 1245 a, c, t dbSNP:745728602
1246 1246 a, g dbSNP:778809798
1250 1250 a, c dbSNP:757200008
1254 1254 a, g dbSNP:749325083
1266 1266 c, t dbSNP:201937527
1271 1271 g, t dbSNP:201753929
1273 1273 g, t dbSNP:780620100
1291 1291 a, g dbSNP:368641715
1293 1293 c, g dbSNP:755394360
1295 1295 a, g dbSNP:151100437
1299 1299 c, g dbSNP:766922660
1300 1300 c, t dbSNP:142899386
1304 1304 c, g dbSNP:148432028
1306 1306 g, t dbSNP:763488544
1309 1309 a, c dbSNP:374451635
1310 1310 c, t dbSNP:538240247
1311 1311 c, t dbSNP:771769935
1317 1317 a, t dbSNP:759269789
1325 1325 g, t dbSNP:17847327
1333 1333 a, g dbSNP:17073229
1336 1336 a, g, t dbSNP:777804198
1343 1343 a, c dbSNP:769737512
1347 1347 c, t dbSNP:748313279
1348 1348 a, t dbSNP:781556516
1351 1351 c, t dbSNP:755267953
1352 1352 a, g dbSNP:751926904
1379 1379 a, g dbSNP:780599925
1383 1383 a, g dbSNP:758839660
1407 1407 a, g dbSNP:750874987
1411 1411 a, g dbSNP:765832081
1419 1419 a, g dbSNP:760099365
1429 1429 g, t dbSNP:752208274
1432 1432 a, g dbSNP:267600848
1474 1474 c, t dbSNP:767098050
1489 1489 c, t dbSNP:141784122
1494 1494 a, t dbSNP:1063413
1510 1510 c, t dbSNP:36120645
1522 1522 a, c dbSNP:547586757
1531 1531 a, g dbSNP:762851758
1532 1532 -, gag dbSNP:760478312
1539 1539 c, t dbSNP:773005838
1545 1545 a, g dbSNP:34652819
1547 1547 a, g dbSNP:748123821
1548 1548 c, g dbSNP:781448211
1555 1555 c, g dbSNP:375679973
1558 1558 c, g, t dbSNP:370449852
1559 1559 a, g dbSNP:758678527
1562 1562 a, g dbSNP:565181543
1565 1565 a, c dbSNP:201977118
1569 1569 c, t dbSNP:377135913
1571 1571 a, c dbSNP:752153135
1583 1583 a, g dbSNP:17847328
1586 1586 a, t dbSNP:754521531
1590 1590 c, t dbSNP:751187671
1596 1596 c, t dbSNP:766110989
1604 1604 -, c dbSNP:35411045
1613 1613 a, g dbSNP:560576837
1621 1621 a, g dbSNP:373156842
1623 1623 c, g dbSNP:142705655
1631 1631 c, t dbSNP:761877385
1644 1644 c, g dbSNP:776753534
1645 1645 c, t dbSNP:768835975
1655 1655 a, g dbSNP:372873338
1656 1656 a, g dbSNP:747145351
1673 1673 c, t dbSNP:775851406
1677 1677 c, g dbSNP:369453748
1679 1679 a, g dbSNP:746160419
1687 1687 c, t dbSNP:200072882
1695 1695 c, t dbSNP:757664610
1696 1696 c, t dbSNP:749761427
1712 1712 a, c dbSNP:778298992
1717 1717 c, t dbSNP:149291080
1718 1718 a, c, g dbSNP:765875826
1719 1719 c, t dbSNP:35263016
1729 1729 a, g dbSNP:578145229
1734 1734 c, t dbSNP:377306571
1735 1735 a, g, t dbSNP:776698636
1741 1741 a, g dbSNP:764195119
1742 1742 c, g dbSNP:141235151
1743 1743 a, c dbSNP:775653923
1744 1744 -, ccagag dbSNP:774596210
1744 1744 a, g dbSNP:772136403
1750 1750 a, g dbSNP:62427100
1751 1751 a, g dbSNP:780626452
1756 1756 c, g dbSNP:374462557
1760 1760 a, c dbSNP:774476162
1762 1762 c, t dbSNP:368929894
1766 1766 c, t dbSNP:556887561
1767 1767 c, t dbSNP:749708141
1768 1768 a, g, t dbSNP:201057231
1769 1769 a, g dbSNP:746453740
1773 1773 c, t dbSNP:779649831
1774 1774 c, t dbSNP:757889837
1780 1780 c, t dbSNP:750070946
1788 1788 c, t dbSNP:200573380
1789 1789 a, g dbSNP:17847330
1803 1803 a, t dbSNP:371698312
1815 1815 a, c dbSNP:147054773
1819 1819 c, t dbSNP:200036472
1820 1820 a, g dbSNP:752677729
1821 1821 c, t dbSNP:767601739
1831 1831 a, t dbSNP:759582013
1838 1838 c, t dbSNP:774617149
1839 1839 c, t dbSNP:771206023
1844 1844 a, g, t dbSNP:141627165
1845 1845 a, c dbSNP:770249452
1852 1852 a, c, g dbSNP:147885532
1855 1855 a, c dbSNP:771601130
1856 1856 -, c dbSNP:768840283
1857 1857 a, t dbSNP:745438962
1859 1859 a, c dbSNP:778643791
1880 1880 c, t dbSNP:756984299
1884 1884 a, g dbSNP:374631467
1894 1894 g, t dbSNP:777741438
1901 1901 a, g dbSNP:756012523
1906 1906 a, g dbSNP:752655598
1927 1927 a, t dbSNP:138688804
1939 1939 c, g dbSNP:759653376
1947 1947 a, g dbSNP:751593481
1955 1955 c, g dbSNP:766525788
1957 1957 a, g dbSNP:763180631
1965 1965 a, g dbSNP:773466984
2000 2000 a, c dbSNP:372153249
2016 2016 a, c dbSNP:762144772
2022 2022 a, c dbSNP:777194150
2025 2025 c, t dbSNP:769049757
2026 2026 a, c dbSNP:200257642
2027 2027 c, t dbSNP:774021173
2036 2036 c, g dbSNP:201957325
2038 2038 a, g dbSNP:72546304
2041 2041 c, t dbSNP:145034248
2044 2044 c, g dbSNP:755923312
2046 2046 a, c, g, t dbSNP:754880138
2047 2047 a, c, g dbSNP:145386261
2048 2048 c, g dbSNP:758526896
2050 2050 a, c, g, t dbSNP:150039945
2051 2051 a, c, g dbSNP:764612601
2052 2052 c, t dbSNP:149400255
2053 2053 c, g dbSNP:761118576
2074 2074 a, c, t dbSNP:17847331
2077 2077 c, t dbSNP:748862556
2082 2082 c, t dbSNP:772992931
2086 2086 c, t dbSNP:769332223
2090 2090 a, c dbSNP:747916100
2091 2091 a, c dbSNP:780866537
2093 2093 a, g dbSNP:751709706
2097 2097 c, t dbSNP:754901227
2103 2103 a, g dbSNP:373757589
2105 2105 a, g dbSNP:780028654
2114 2114 a, g dbSNP:758471604
2115 2115 c, t dbSNP:750509016
2122 2122 a, g dbSNP:765442934
2123 2123 -, cctgggg dbSNP:749424025
2123 2123 a, c dbSNP:757445503
2126 2126 a, g dbSNP:754192424
2131 2131 a, c dbSNP:370253986
2135 2135 c, t dbSNP:548577685
2150 2150 a, c dbSNP:776125291
2151 2151 a, g dbSNP:764191640
2157 2157 a, t dbSNP:768204588
2158 2158 a, g dbSNP:762482586
2161 2161 g, t dbSNP:772859501
2163 2163 a, g dbSNP:769435521
2169 2169 a, c dbSNP:374421447
2170 2170 g, t dbSNP:776397265
2172 2172 -, gca dbSNP:775540928
2174 2174 -, c dbSNP:35716705
2178 2178 c, t dbSNP:768326522
2189 2189 a, g dbSNP:746874822
2191 2191 a, c, g dbSNP:527350024
2207 2207 a, g dbSNP:745900352
2227 2227 c, t dbSNP:778890400
2232 2232 a, c dbSNP:36087014
2235 2235 a, g dbSNP:757399152
2245 2245 a, t dbSNP:753984271
2263 2263 c, t dbSNP:778115846
2266 2266 c, t dbSNP:756540757
2269 2269 a, g dbSNP:370394316
2275 2275 c, t dbSNP:768076125
2300 2300 a, g dbSNP:760139789
2301 2301 c, t dbSNP:749905224
2305 2305 a, t dbSNP:764618922
2311 2311 a, g dbSNP:761447530
2314 2314 a, t dbSNP:776147456
2316 2316 -, attt dbSNP:769843457
2319 2319 c, t dbSNP:560190372
2320 2320 a, g dbSNP:146546302
2330 2330 a, g dbSNP:775287768
2331 2331 g, t dbSNP:771959962
2334 2334 -, atg dbSNP:746386623
2334 2334 a, g dbSNP:745775222
2377 2377 g, t dbSNP:769371400
2385 2385 -, a dbSNP:563450219
2386 2386 c, t dbSNP:150413533
2417 2417 a, g dbSNP:3544
2432 2432 -, gat dbSNP:552001777
2434 2434 -, aga dbSNP:112027877
2468 2468 c, t dbSNP:753866564
2507 2507 a, g dbSNP:373528883
2587 2587 c, t dbSNP:766182254
2638 2638 a, g dbSNP:775132795
2661 2661 c, t dbSNP:140595869
2688 2688 a, g dbSNP:765027393
2724 2724 g, t dbSNP:759398749
2760 2760 a, g dbSNP:541432527
2778 2778 a, g dbSNP:544894567
2781 2781 a, g dbSNP:1804733
2809 2809 a, c dbSNP:151333282
2860 2860 -, t dbSNP:376801986
2904 2904 a, g dbSNP:142469019
2936 2936 c, t dbSNP:780234856
2974 2974 a, c, g dbSNP:200779763
2976 2976 -, a dbSNP:397747580
2990 2990 -, a dbSNP:35981443
3027 3027 a, g dbSNP:373423492
3029 3029 c, t dbSNP:573194607
3048 3048 g, t dbSNP:558214787
3061 3061 g, t dbSNP:373013057
3077 3077 c, t dbSNP:539575179
3111 3111 a, t dbSNP:571080195
3137 3137 c, t dbSNP:770374188
3145 3145 g, t dbSNP:11556960
3214 3214 g, t dbSNP:563141520
3262 3262 c, t dbSNP:555954627
3333 3333 c, t dbSNP:537706046
3347 3347 c, t dbSNP:558334072
3377 3377 a, g dbSNP:1049846
3417 3417 a, g dbSNP:12417

Target ORF information:

RefSeq Version NM_001080952
Organism Homo sapiens (human)
Definition Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001080952

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCCACGT TCCCCTGCCA GTTATGTGGC AAGACGTTCC TCACCCTGGA GAAGTTCACG 
ATTCACAATT ATTCCCACTC CAGGGAGCGG CCGTACAAGT GTGTGCAGCC TGACTGTGGC
AAAGCCTTTG TTTCCAGATA TAAATTGATG AGGCATATGG CTACCCATTC TCCCCAGAAA
TCTCACCAGT GTGCTCACTG TGAGAAGACG TTCAACCGGA AAGACCACCT GAAAAACCAC
CTCCAGACCC ACGACCCCAA CAAAATGGCC TTTGGGTGTG AGGAGTGTGG GAAGAAGTAC
AACACCATGC TGGGCTATAA GAGGCACCTG GCCCTCCATG CGGCCAGCAG TGGGGACCTC
ACCTGTGGGG TCTGTGCCCT GGAGCTAGGG AGCACCGAGG TGCTACTGGA CCACCTCAAA
GCCCATGCGG AAGAGAAGCC CCCTAGCGGA ACCAAGGAAA AGAAGCACCA GTGCGACCAC
TGTGAAAGAT GCTTCTACAC CCGGAAGGAT GTGCGACGCC ACCTGGTGGT CCACACAGGA
TGCAAGGACT TCCTGTGCCA GTTCTGTGCC CAGAGATTTG GGCGCAAGGA TCACCTCACC
CGGCATACCA AGAAGACCCA CTCACAGGAG CTGATGAAAG AGAGCTTGCA GACCGGAGAC
CTTCTGAGCA CCTTCCACAC CATCTCGCCT TCATTCCAAC TGAAGGCTGC TGCCTTGCCT
CCTTTCCCTT TAGGAGCTTC TGCCCAGAAC GGGCTTGCAA GTAGCTTGCC AGCTGAGGTC
CATAGCCTCA CCCTCAGTCC CCCAGAACAA GCCGCCCAGC CTATGCAGCC GCTGCCAGAG
TCCCTGGCCT CCCTCCACCC CTCGGTATCC CCTGGCTCTC CTCCGCCACC CCTTCCCAAT
CACAAGTACA ACACCACTTC TACCTCATAC TCCCCACTTG CAAGCCTGCC CCTCAAAGCA
GATACTAAAG GTTTTTGCAA TATCAGTTTG TTTGAGGACT TGCCTCTGCA AGAGCCTCAG
TCACCTCAAA AGCTCAACCC AGGTTTTGAT CTGGCTAAGG GAAATGCTGG TAAAGTAAAC
CTGCCCAAGG AGCTGCCTGC AGATGCTGTG AACCTAACAA TACCTGCCTC TCTGGACCTG
TCCCCCCTGT TGGGCTTCTG GCAGCTGCCC CCTCCTGCTA CCCAAAATAC CTTTGGGAAT
AGCACTCTTG CCCTGGGGCC TGGGGAATCT TTGCCCCACA GGTTAAGCTG TCTGGGGCAG
CAGCAGCAAG AACCCCCACT TGCCATGGGC ACTGTGAGCC TGGGCCAGCT CCCCCTGCCC
CCCATCCCTC ATGTGTTCTC AGCTGGCACT GGCTCTGCCA TCCTGCCTCA TTTCCATCAT
GCATTCAGAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19858
Accession Version NM_001080953.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1392bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product zinc finger protein PLAGL1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA446007.1, DA549753.1, EF100450.1, EF100448.1, AJ311395.1 and AI741398.1. Summary: This gene encodes a C2H2 zinc finger protein with transactivation and DNA-binding activities. It has been shown to have anti-proliferative properties, and thus thought to function as a tumor suppressor. In addition, overexpression of this gene during fetal development is believed to underlie the rare disorder, transient neonatal diabetes mellitus (TNDM). This gene is imprinted, with preferential expression of the paternal allele in many tissues, however, biallelic expression has been noted in peripheral blood leucocytes. A recent study reports that tissue-specific imprinting results from variable utilization of monoallelic and biallelic promoters. Many transcript variants differing in the 5' UTR and encoding two different isoforms, have been found for this gene. [provided by RefSeq, Oct 2010]. Transcript Variant: This variant (5) differs in the 5' UTR and contains an alternate exon in the CDS compared to variant 1. The resulting isoform (2) is longer and has an extended N-terminus compared to isoform 1. Variants 2, 3, 4, 5, 6, 10, 12, 15, 16, 17, 18, 19 and 20 all encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## imprinted gene :: PMID: 17341487, 10655556 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: EF100450.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCCACGT TCCCCTGCCA GTTATGTGGC AAGACGTTCC TCACCCTGGA GAAGTTCACG 
ATTCACAATT ATTCCCACTC CAGGGAGCGG CCGTACAAGT GTGTGCAGCC TGACTGTGGC
AAAGCCTTTG TTTCCAGATA TAAATTGATG AGGCATATGG CTACCCATTC TCCCCAGAAA
TCTCACCAGT GTGCTCACTG TGAGAAGACG TTCAACCGGA AAGACCACCT GAAAAACCAC
CTCCAGACCC ACGACCCCAA CAAAATGGCC TTTGGGTGTG AGGAGTGTGG GAAGAAGTAC
AACACCATGC TGGGCTATAA GAGGCACCTG GCCCTCCATG CGGCCAGCAG TGGGGACCTC
ACCTGTGGGG TCTGTGCCCT GGAGCTAGGG AGCACCGAGG TGCTACTGGA CCACCTCAAA
GCCCATGCGG AAGAGAAGCC CCCTAGCGGA ACCAAGGAAA AGAAGCACCA GTGCGACCAC
TGTGAAAGAT GCTTCTACAC CCGGAAGGAT GTGCGACGCC ACCTGGTGGT CCACACAGGA
TGCAAGGACT TCCTGTGCCA GTTCTGTGCC CAGAGATTTG GGCGCAAGGA TCACCTCACC
CGGCATACCA AGAAGACCCA CTCACAGGAG CTGATGAAAG AGAGCTTGCA GACCGGAGAC
CTTCTGAGCA CCTTCCACAC CATCTCGCCT TCATTCCAAC TGAAGGCTGC TGCCTTGCCT
CCTTTCCCTT TAGGAGCTTC TGCCCAGAAC GGGCTTGCAA GTAGCTTGCC AGCTGAGGTC
CATAGCCTCA CCCTCAGTCC CCCAGAACAA GCCGCCCAGC CTATGCAGCC GCTGCCAGAG
TCCCTGGCCT CCCTCCACCC CTCGGTATCC CCTGGCTCTC CTCCGCCACC CCTTCCCAAT
CACAAGTACA ACACCACTTC TACCTCATAC TCCCCACTTG CAAGCCTGCC CCTCAAAGCA
GATACTAAAG GTTTTTGCAA TATCAGTTTG TTTGAGGACT TGCCTCTGCA AGAGCCTCAG
TCACCTCAAA AGCTCAACCC AGGTTTTGAT CTGGCTAAGG GAAATGCTGG TAAAGTAAAC
CTGCCCAAGG AGCTGCCTGC AGATGCTGTG AACCTAACAA TACCTGCCTC TCTGGACCTG
TCCCCCCTGT TGGGCTTCTG GCAGCTGCCC CCTCCTGCTA CCCAAAATAC CTTTGGGAAT
AGCACTCTTG CCCTGGGGCC TGGGGAATCT TTGCCCCACA GGTTAAGCTG TCTGGGGCAG
CAGCAGCAAG AACCCCCACT TGCCATGGGC ACTGTGAGCC TGGGCCAGCT CCCCCTGCCC
CCCATCCCTC ATGTGTTCTC AGCTGGCACT GGCTCTGCCA TCCTGCCTCA TTTCCATCAT
GCATTCAGAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001074422.1
CDS859..2250
Misc Feature(1)844..846(+)
Misc Feature(2)874..936(+)
Misc Feature(3)874..936(+)
Misc Feature(4)889..1107(+)
Misc Feature(5)958..1026(+)
Misc Feature(6)958..1023(+)
Misc Feature(7)1003..1077(+)
Misc Feature(8)1048..1110(+)
Misc Feature(9)1048..1110(+)
Misc Feature(10)1048..1110(+)
Misc Feature(11)1048..1110(+)
Misc Feature(12)1063..1194(+)
Misc Feature(13)1135..1197(+)
Misc Feature(14)1135..1197(+)
Misc Feature(15)1222..1284(+)
Misc Feature(16)1222..1284(+)
Misc Feature(17)1237..1476(+)
Misc Feature(18)1330..1392(+)
Misc Feature(19)1330..1389(+)
Misc Feature(20)1414..1479(+)
Misc Feature(21)1414..1479(+)
Exon (1)1..315
Gene:PLAGL1
Gene Synonym:
Exon (2)316..387
Gene:PLAGL1
Gene Synonym:
Exon (3)388..428
Gene:PLAGL1
Gene Synonym:
Exon (4)429..460
Gene:PLAGL1
Gene Synonym:
Exon (5)461..534
Gene:PLAGL1
Gene Synonym:
Exon (6)535..1010
Gene:PLAGL1
Gene Synonym:
Exon (7)1011..3374
Gene:PLAGL1
Gene Synonym:
Translation
Position Chain Variation Link
98 98 a, g dbSNP:549107424
130 130 a, t dbSNP:188083405
180 180 a, g dbSNP:113189219
264 264 a, g dbSNP:779493627
279 279 a, g dbSNP:67300369
287 287 c, t dbSNP:749380636
368 368 a, t dbSNP:543763464
381 381 c, t dbSNP:573299088
389 389 c, t dbSNP:192562088
402 402 a, g dbSNP:368424368
437 437 a, t dbSNP:370220800
494 494 c, t dbSNP:553367951
495 495 a, c, g dbSNP:570633358
507 507 a, g dbSNP:551127170
591 591 c, g dbSNP:2076684
678 678 c, t dbSNP:370977690
682 682 g, t dbSNP:77502692
719 719 c, t dbSNP:138964933
721 721 c, t dbSNP:745769302
722 722 a, g dbSNP:571326035
736 736 c, t dbSNP:549961074
737 737 a, g dbSNP:191543871
747 747 a, g dbSNP:549484273
783 783 c, t dbSNP:531468151
787 787 a, g dbSNP:562539391
790 790 c, t dbSNP:145922363
846 846 a, c dbSNP:750512755
854 854 a, g dbSNP:765374630
866 866 a, c, t dbSNP:529372879
867 867 a, g dbSNP:539999694
868 868 c, t dbSNP:147037984
891 891 a, g dbSNP:776060847
894 894 a, g dbSNP:141506249
903 903 c, t dbSNP:772513575
906 906 c, g dbSNP:147962624
915 915 c, g dbSNP:777429105
917 917 c, t dbSNP:769533076
918 918 a, g dbSNP:201628824
946 946 c, t dbSNP:780760124
947 947 a, g, t dbSNP:201696236
951 951 a, g dbSNP:565249616
952 952 a, t dbSNP:758334227
956 956 a, c dbSNP:750460388
957 957 a, g dbSNP:377168292
976 976 c, g dbSNP:761929176
982 982 a, c, g dbSNP:371685096
994 994 a, g dbSNP:761089335
995 995 c, g dbSNP:775732313
1002 1002 a, g dbSNP:367667352
1003 1003 a, c, t dbSNP:186779775
1022 1022 a, c dbSNP:758281178
1056 1056 c, t dbSNP:745781639
1064 1064 -, aga dbSNP:776571919
1068 1068 a, g dbSNP:144954752
1075 1075 c, t dbSNP:757324170
1076 1076 a, g dbSNP:753924230
1110 1110 c, t dbSNP:764320213
1111 1111 a, g, t dbSNP:753068252
1118 1118 a, g dbSNP:768018756
1119 1119 a, c dbSNP:760031131
1121 1121 -, caa dbSNP:766108577
1125 1125 a, g dbSNP:774971840
1133 1133 a, g dbSNP:139967852
1138 1138 a, g dbSNP:767063830
1140 1140 a, g dbSNP:761345864
1155 1155 a, g dbSNP:776006127
1159 1159 a, c dbSNP:768311718
1160 1160 a, c dbSNP:746660705
1182 1182 a, g dbSNP:775255229
1199 1199 a, c, t dbSNP:745728602
1200 1200 a, g dbSNP:778809798
1204 1204 a, c dbSNP:757200008
1208 1208 a, g dbSNP:749325083
1220 1220 c, t dbSNP:201937527
1225 1225 g, t dbSNP:201753929
1227 1227 g, t dbSNP:780620100
1245 1245 a, g dbSNP:368641715
1247 1247 c, g dbSNP:755394360
1249 1249 a, g dbSNP:151100437
1253 1253 c, g dbSNP:766922660
1254 1254 c, t dbSNP:142899386
1258 1258 c, g dbSNP:148432028
1260 1260 g, t dbSNP:763488544
1263 1263 a, c dbSNP:374451635
1264 1264 c, t dbSNP:538240247
1265 1265 c, t dbSNP:771769935
1271 1271 a, t dbSNP:759269789
1279 1279 g, t dbSNP:17847327
1287 1287 a, g dbSNP:17073229
1290 1290 a, g, t dbSNP:777804198
1297 1297 a, c dbSNP:769737512
1301 1301 c, t dbSNP:748313279
1302 1302 a, t dbSNP:781556516
1305 1305 c, t dbSNP:755267953
1306 1306 a, g dbSNP:751926904
1333 1333 a, g dbSNP:780599925
1337 1337 a, g dbSNP:758839660
1361 1361 a, g dbSNP:750874987
1365 1365 a, g dbSNP:765832081
1373 1373 a, g dbSNP:760099365
1383 1383 g, t dbSNP:752208274
1386 1386 a, g dbSNP:267600848
1428 1428 c, t dbSNP:767098050
1443 1443 c, t dbSNP:141784122
1448 1448 a, t dbSNP:1063413
1464 1464 c, t dbSNP:36120645
1476 1476 a, c dbSNP:547586757
1485 1485 a, g dbSNP:762851758
1486 1486 -, gag dbSNP:760478312
1493 1493 c, t dbSNP:773005838
1499 1499 a, g dbSNP:34652819
1501 1501 a, g dbSNP:748123821
1502 1502 c, g dbSNP:781448211
1509 1509 c, g dbSNP:375679973
1512 1512 c, g, t dbSNP:370449852
1513 1513 a, g dbSNP:758678527
1516 1516 a, g dbSNP:565181543
1519 1519 a, c dbSNP:201977118
1523 1523 c, t dbSNP:377135913
1525 1525 a, c dbSNP:752153135
1537 1537 a, g dbSNP:17847328
1540 1540 a, t dbSNP:754521531
1544 1544 c, t dbSNP:751187671
1550 1550 c, t dbSNP:766110989
1558 1558 -, c dbSNP:35411045
1567 1567 a, g dbSNP:560576837
1575 1575 a, g dbSNP:373156842
1577 1577 c, g dbSNP:142705655
1585 1585 c, t dbSNP:761877385
1598 1598 c, g dbSNP:776753534
1599 1599 c, t dbSNP:768835975
1609 1609 a, g dbSNP:372873338
1610 1610 a, g dbSNP:747145351
1627 1627 c, t dbSNP:775851406
1631 1631 c, g dbSNP:369453748
1633 1633 a, g dbSNP:746160419
1641 1641 c, t dbSNP:200072882
1649 1649 c, t dbSNP:757664610
1650 1650 c, t dbSNP:749761427
1666 1666 a, c dbSNP:778298992
1671 1671 c, t dbSNP:149291080
1672 1672 a, c, g dbSNP:765875826
1673 1673 c, t dbSNP:35263016
1683 1683 a, g dbSNP:578145229
1688 1688 c, t dbSNP:377306571
1689 1689 a, g, t dbSNP:776698636
1695 1695 a, g dbSNP:764195119
1696 1696 c, g dbSNP:141235151
1697 1697 a, c dbSNP:775653923
1698 1698 -, ccagag dbSNP:774596210
1698 1698 a, g dbSNP:772136403
1704 1704 a, g dbSNP:62427100
1705 1705 a, g dbSNP:780626452
1710 1710 c, g dbSNP:374462557
1714 1714 a, c dbSNP:774476162
1716 1716 c, t dbSNP:368929894
1720 1720 c, t dbSNP:556887561
1721 1721 c, t dbSNP:749708141
1722 1722 a, g, t dbSNP:201057231
1723 1723 a, g dbSNP:746453740
1727 1727 c, t dbSNP:779649831
1728 1728 c, t dbSNP:757889837
1734 1734 c, t dbSNP:750070946
1742 1742 c, t dbSNP:200573380
1743 1743 a, g dbSNP:17847330
1757 1757 a, t dbSNP:371698312
1769 1769 a, c dbSNP:147054773
1773 1773 c, t dbSNP:200036472
1774 1774 a, g dbSNP:752677729
1775 1775 c, t dbSNP:767601739
1785 1785 a, t dbSNP:759582013
1792 1792 c, t dbSNP:774617149
1793 1793 c, t dbSNP:771206023
1798 1798 a, g, t dbSNP:141627165
1799 1799 a, c dbSNP:770249452
1806 1806 a, c, g dbSNP:147885532
1809 1809 a, c dbSNP:771601130
1810 1810 -, c dbSNP:768840283
1811 1811 a, t dbSNP:745438962
1813 1813 a, c dbSNP:778643791
1834 1834 c, t dbSNP:756984299
1838 1838 a, g dbSNP:374631467
1848 1848 g, t dbSNP:777741438
1855 1855 a, g dbSNP:756012523
1860 1860 a, g dbSNP:752655598
1881 1881 a, t dbSNP:138688804
1893 1893 c, g dbSNP:759653376
1901 1901 a, g dbSNP:751593481
1909 1909 c, g dbSNP:766525788
1911 1911 a, g dbSNP:763180631
1919 1919 a, g dbSNP:773466984
1954 1954 a, c dbSNP:372153249
1970 1970 a, c dbSNP:762144772
1976 1976 a, c dbSNP:777194150
1979 1979 c, t dbSNP:769049757
1980 1980 a, c dbSNP:200257642
1981 1981 c, t dbSNP:774021173
1990 1990 c, g dbSNP:201957325
1992 1992 a, g dbSNP:72546304
1995 1995 c, t dbSNP:145034248
1998 1998 c, g dbSNP:755923312
2000 2000 a, c, g, t dbSNP:754880138
2001 2001 a, c, g dbSNP:145386261
2002 2002 c, g dbSNP:758526896
2004 2004 a, c, g, t dbSNP:150039945
2005 2005 a, c, g dbSNP:764612601
2006 2006 c, t dbSNP:149400255
2007 2007 c, g dbSNP:761118576
2028 2028 a, c, t dbSNP:17847331
2031 2031 c, t dbSNP:748862556
2036 2036 c, t dbSNP:772992931
2040 2040 c, t dbSNP:769332223
2044 2044 a, c dbSNP:747916100
2045 2045 a, c dbSNP:780866537
2047 2047 a, g dbSNP:751709706
2051 2051 c, t dbSNP:754901227
2057 2057 a, g dbSNP:373757589
2059 2059 a, g dbSNP:780028654
2068 2068 a, g dbSNP:758471604
2069 2069 c, t dbSNP:750509016
2076 2076 a, g dbSNP:765442934
2077 2077 -, cctgggg dbSNP:749424025
2077 2077 a, c dbSNP:757445503
2080 2080 a, g dbSNP:754192424
2085 2085 a, c dbSNP:370253986
2089 2089 c, t dbSNP:548577685
2104 2104 a, c dbSNP:776125291
2105 2105 a, g dbSNP:764191640
2111 2111 a, t dbSNP:768204588
2112 2112 a, g dbSNP:762482586
2115 2115 g, t dbSNP:772859501
2117 2117 a, g dbSNP:769435521
2123 2123 a, c dbSNP:374421447
2124 2124 g, t dbSNP:776397265
2126 2126 -, gca dbSNP:775540928
2128 2128 -, c dbSNP:35716705
2132 2132 c, t dbSNP:768326522
2143 2143 a, g dbSNP:746874822
2145 2145 a, c, g dbSNP:527350024
2161 2161 a, g dbSNP:745900352
2181 2181 c, t dbSNP:778890400
2186 2186 a, c dbSNP:36087014
2189 2189 a, g dbSNP:757399152
2199 2199 a, t dbSNP:753984271
2217 2217 c, t dbSNP:778115846
2220 2220 c, t dbSNP:756540757
2223 2223 a, g dbSNP:370394316
2229 2229 c, t dbSNP:768076125
2254 2254 a, g dbSNP:760139789
2255 2255 c, t dbSNP:749905224
2259 2259 a, t dbSNP:764618922
2265 2265 a, g dbSNP:761447530
2268 2268 a, t dbSNP:776147456
2270 2270 -, attt dbSNP:769843457
2273 2273 c, t dbSNP:560190372
2274 2274 a, g dbSNP:146546302
2284 2284 a, g dbSNP:775287768
2285 2285 g, t dbSNP:771959962
2288 2288 -, atg dbSNP:746386623
2288 2288 a, g dbSNP:745775222
2331 2331 g, t dbSNP:769371400
2339 2339 -, a dbSNP:563450219
2340 2340 c, t dbSNP:150413533
2371 2371 a, g dbSNP:3544
2386 2386 -, gat dbSNP:552001777
2388 2388 -, aga dbSNP:112027877
2422 2422 c, t dbSNP:753866564
2461 2461 a, g dbSNP:373528883
2541 2541 c, t dbSNP:766182254
2592 2592 a, g dbSNP:775132795
2615 2615 c, t dbSNP:140595869
2642 2642 a, g dbSNP:765027393
2678 2678 g, t dbSNP:759398749
2714 2714 a, g dbSNP:541432527
2732 2732 a, g dbSNP:544894567
2735 2735 a, g dbSNP:1804733
2763 2763 a, c dbSNP:151333282
2814 2814 -, t dbSNP:376801986
2858 2858 a, g dbSNP:142469019
2890 2890 c, t dbSNP:780234856
2928 2928 a, c, g dbSNP:200779763
2930 2930 -, a dbSNP:397747580
2944 2944 -, a dbSNP:35981443
2981 2981 a, g dbSNP:373423492
2983 2983 c, t dbSNP:573194607
3002 3002 g, t dbSNP:558214787
3015 3015 g, t dbSNP:373013057
3031 3031 c, t dbSNP:539575179
3065 3065 a, t dbSNP:571080195
3091 3091 c, t dbSNP:770374188
3099 3099 g, t dbSNP:11556960
3168 3168 g, t dbSNP:563141520
3216 3216 c, t dbSNP:555954627
3287 3287 c, t dbSNP:537706046
3301 3301 c, t dbSNP:558334072
3331 3331 a, g dbSNP:1049846
3371 3371 a, g dbSNP:12417

Target ORF information:

RefSeq Version NM_001080953
Organism Homo sapiens (human)
Definition Homo sapiens pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001080953

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCCACGT TCCCCTGCCA GTTATGTGGC AAGACGTTCC TCACCCTGGA GAAGTTCACG 
ATTCACAATT ATTCCCACTC CAGGGAGCGG CCGTACAAGT GTGTGCAGCC TGACTGTGGC
AAAGCCTTTG TTTCCAGATA TAAATTGATG AGGCATATGG CTACCCATTC TCCCCAGAAA
TCTCACCAGT GTGCTCACTG TGAGAAGACG TTCAACCGGA AAGACCACCT GAAAAACCAC
CTCCAGACCC ACGACCCCAA CAAAATGGCC TTTGGGTGTG AGGAGTGTGG GAAGAAGTAC
AACACCATGC TGGGCTATAA GAGGCACCTG GCCCTCCATG CGGCCAGCAG TGGGGACCTC
ACCTGTGGGG TCTGTGCCCT GGAGCTAGGG AGCACCGAGG TGCTACTGGA CCACCTCAAA
GCCCATGCGG AAGAGAAGCC CCCTAGCGGA ACCAAGGAAA AGAAGCACCA GTGCGACCAC
TGTGAAAGAT GCTTCTACAC CCGGAAGGAT GTGCGACGCC ACCTGGTGGT CCACACAGGA
TGCAAGGACT TCCTGTGCCA GTTCTGTGCC CAGAGATTTG GGCGCAAGGA TCACCTCACC
CGGCATACCA AGAAGACCCA CTCACAGGAG CTGATGAAAG AGAGCTTGCA GACCGGAGAC
CTTCTGAGCA CCTTCCACAC CATCTCGCCT TCATTCCAAC TGAAGGCTGC TGCCTTGCCT
CCTTTCCCTT TAGGAGCTTC TGCCCAGAAC GGGCTTGCAA GTAGCTTGCC AGCTGAGGTC
CATAGCCTCA CCCTCAGTCC CCCAGAACAA GCCGCCCAGC CTATGCAGCC GCTGCCAGAG
TCCCTGGCCT CCCTCCACCC CTCGGTATCC CCTGGCTCTC CTCCGCCACC CCTTCCCAAT
CACAAGTACA ACACCACTTC TACCTCATAC TCCCCACTTG CAAGCCTGCC CCTCAAAGCA
GATACTAAAG GTTTTTGCAA TATCAGTTTG TTTGAGGACT TGCCTCTGCA AGAGCCTCAG
TCACCTCAAA AGCTCAACCC AGGTTTTGAT CTGGCTAAGG GAAATGCTGG TAAAGTAAAC
CTGCCCAAGG AGCTGCCTGC AGATGCTGTG AACCTAACAA TACCTGCCTC TCTGGACCTG
TCCCCCCTGT TGGGCTTCTG GCAGCTGCCC CCTCCTGCTA CCCAAAATAC CTTTGGGAAT
AGCACTCTTG CCCTGGGGCC TGGGGAATCT TTGCCCCACA GGTTAAGCTG TCTGGGGCAG
CAGCAGCAAG AACCCCCACT TGCCATGGGC ACTGTGAGCC TGGGCCAGCT CCCCCTGCCC
CCCATCCCTC ATGTGTTCTC AGCTGGCACT GGCTCTGCCA TCCTGCCTCA TTTCCATCAT
GCATTCAGAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19858
Accession Version NM_001080954.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1392bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product zinc finger protein PLAGL1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA446007.1, DA549753.1, EF100449.1, DA595200.1, EF100448.1, AJ311395.1 and AI741398.1. Summary: This gene encodes a C2H2 zinc finger protein with transactivation and DNA-binding activities. It has been shown to have anti-proliferative properties, and thus thought to function as a tumor suppressor. In addition, overexpression of this gene during fetal development is believed to underlie the rare disorder, transient neonatal diabetes mellitus (TNDM). This gene is imprinted, with preferential expression of the paternal allele in many tissues, however, biallelic expression has been noted in peripheral blood leucocytes. A recent study reports that tissue-specific imprinting results from variable utilization of monoallelic and biallelic promoters. Many transcript variants differing in the 5' UTR and encoding two different isoforms, have been found for this gene. [provided by RefSeq, Oct 2010]. Transcript Variant: This variant (6) differs in the 5' UTR and contains an alternate exon in the CDS compared to variant 1. The resulting isoform (2) is longer and has an extended N-terminus compared to isoform 1. Variants 2, 3, 4, 5, 6, 10, 12, 15, 16, 17, 18, 19 and 20 all encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## imprinted gene :: PMID: 17341487, 10655556 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: EF100449.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162568 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCCACGT TCCCCTGCCA GTTATGTGGC AAGACGTTCC TCACCCTGGA GAAGTTCACG 
ATTCACAATT ATTCCCACTC CAGGGAGCGG CCGTACAAGT GTGTGCAGCC TGACTGTGGC
AAAGCCTTTG TTTCCAGATA TAAATTGATG AGGCATATGG CTACCCATTC TCCCCAGAAA
TCTCACCAGT GTGCTCACTG TGAGAAGACG TTCAACCGGA AAGACCACCT GAAAAACCAC
CTCCAGACCC ACGACCCCAA CAAAATGGCC TTTGGGTGTG AGGAGTGTGG GAAGAAGTAC
AACACCATGC TGGGCTATAA GAGGCACCTG GCCCTCCATG CGGCCAGCAG TGGGGACCTC
ACCTGTGGGG TCTGTGCCCT GGAGCTAGGG AGCACCGAGG TGCTACTGGA CCACCTCAAA
GCCCATGCGG AAGAGAAGCC CCCTAGCGGA ACCAAGGAAA AGAAGCACCA GTGCGACCAC
TGTGAAAGAT GCTTCTACAC CCGGAAGGAT GTGCGACGCC ACCTGGTGGT CCACACAGGA
TGCAAGGACT TCCTGTGCCA GTTCTGTGCC CAGAGATTTG GGCGCAAGGA TCACCTCACC
CGGCATACCA AGAAGACCCA CTCACAGGAG CTGATGAAAG AGAGCTTGCA GACCGGAGAC
CTTCTGAGCA CCTTCCACAC CATCTCGCCT TCATTCCAAC TGAAGGCTGC TGCCTTGCCT
CCTTTCCCTT TAGGAGCTTC TGCCCAGAAC GGGCTTGCAA GTAGCTTGCC AGCTGAGGTC
CATAGCCTCA CCCTCAGTCC CCCAGAACAA GCCGCCCAGC CTATGCAGCC GCTGCCAGAG
TCCCTGGCCT CCCTCCACCC CTCGGTATCC CCTGGCTCTC CTCCGCCACC CCTTCCCAAT
CACAAGTACA ACACCACTTC TACCTCATAC TCCCCACTTG CAAGCCTGCC CCTCAAAGCA
GATACTAAAG GTTTTTGCAA TATCAGTTTG TTTGAGGACT TGCCTCTGCA AGAGCCTCAG
TCACCTCAAA AGCTCAACCC AGGTTTTGAT CTGGCTAAGG GAAATGCTGG TAAAGTAAAC
CTGCCCAAGG AGCTGCCTGC AGATGCTGTG AACCTAACAA TACCTGCCTC TCTGGACCTG
TCCCCCCTGT TGGGCTTCTG GCAGCTGCCC CCTCCTGCTA CCCAAAATAC CTTTGGGAAT
AGCACTCTTG CCCTGGGGCC TGGGGAATCT TTGCCCCACA GGTTAAGCTG TCTGGGGCAG
CAGCAGCAAG AACCCCCACT TGCCATGGGC ACTGTGAGCC TGGGCCAGCT CCCCCTGCCC
CCCATCCCTC ATGTGTTCTC AGCTGGCACT GGCTCTGCCA TCCTGCCTCA TTTCCATCAT
GCATTCAGAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001074423.1
CDS858..2249
Misc Feature(1)843..845(+)
Misc Feature(2)873..935(+)
Misc Feature(3)873..935(+)
Misc Feature(4)888..1106(+)
Misc Feature(5)957..1025(+)
Misc Feature(6)957..1022(+)
Misc Feature(7)1002..1076(+)
Misc Feature(8)1047..1109(+)
Misc Feature(9)1047..1109(+)
Misc Feature(10)1047..1109(+)
Misc Feature(11)1047..1109(+)
Misc Feature(12)1062..1193(+)
Misc Feature(13)1134..1196(+)
Misc Feature(14)1134..1196(+)
Misc Feature(15)1221..1283(+)
Misc Feature(16)1221..1283(+)
Misc Feature(17)1236..1475(+)
Misc Feature(18)1329..1391(+)
Misc Feature(19)1329..1388(+)
Misc Feature(20)1413..1478(+)
Misc Feature(21)1413..1478(+)
Exon (1)1..315
Gene:PLAGL1
Gene Synonym:
Exon (2)316..355
Gene:PLAGL1
Gene Synonym:
Exon (3)356..427
Gene:PLAGL1
Gene Synonym:
Exon (4)428..459
Gene:PLAGL1
Gene Synonym:
Exon (5)460..533
Gene:PLAGL1
Gene Synonym:
Exon (6)534..1009
Gene:PLAGL1
Gene Synonym:
Exon (7)1010..3373
Gene:PLAGL1
Gene Synonym:
Translation
Position Chain Variation Link
98 98 a, g dbSNP:549107424
130 130 a, t dbSNP:188083405
180 180 a, g dbSNP:113189219
264 264 a, g dbSNP:779493627
279 279 a, g dbSNP:67300369
287 287 c, t dbSNP:749380636
333 333 a, c dbSNP:182593182
408 408 a, t dbSNP:543763464
421 421 c, t dbSNP:573299088
436 436 a, t dbSNP:370220800
493 493 c, t dbSNP:553367951
494 494 a, c, g dbSNP:570633358
506 506 a, g dbSNP:551127170
590 590 c, g dbSNP:2076684
677 677 c, t dbSNP:370977690
681 681 g, t dbSNP:77502692
718 718 c, t dbSNP:138964933
720 720 c, t dbSNP:745769302
721 721 a, g dbSNP:571326035
735 735 c, t dbSNP:549961074
736 736 a, g dbSNP:191543871
746 746 a, g dbSNP:549484273
782 782 c, t dbSNP:531468151
786 786 a, g dbSNP:562539391
789 789 c, t dbSNP:145922363
845 845 a, c dbSNP:750512755
853 853 a, g dbSNP:765374630
865 865 a, c, t dbSNP:529372879
866 866 a, g dbSNP:539999694
867 867 c, t dbSNP:147037984
890 890 a, g dbSNP:776060847
893 893 a, g dbSNP:141506249
902 902 c, t dbSNP:772513575
905 905 c, g dbSNP:147962624
914 914 c, g dbSNP:777429105
916 916 c, t dbSNP:769533076
917 917 a, g dbSNP:201628824
945 945 c, t dbSNP:780760124
946 946 a, g, t dbSNP:201696236
950 950 a, g dbSNP:565249616
951 951 a, t dbSNP:758334227
955 955 a, c dbSNP:750460388
956 956 a, g dbSNP:377168292
975 975 c, g dbSNP:761929176
981 981 a, c, g dbSNP:371685096
993 993 a, g dbSNP:761089335
994 994 c, g dbSNP:775732313
1001 1001 a, g dbSNP:367667352
1002 1002 a, c, t dbSNP:186779775
1021 1021 a, c dbSNP:758281178
1055 1055 c, t dbSNP:745781639
1063 1063 -, aga dbSNP:776571919
1067 1067 a, g dbSNP:144954752
1074 1074 c, t dbSNP:757324170
1075 1075 a, g dbSNP:753924230
1109 1109 c, t dbSNP:764320213
1110 1110 a, g, t dbSNP:753068252
1117 1117 a, g dbSNP:768018756
1118 1118 a, c dbSNP:760031131
1120 1120 -, caa dbSNP:766108577
1124 1124 a, g dbSNP:774971840
1132 1132 a, g dbSNP:139967852
1137 1137 a, g dbSNP:767063830
1139 1139 a, g dbSNP:761345864
1154 1154 a, g dbSNP:776006127
1158 1158 a, c dbSNP:768311718
1159 1159 a, c dbSNP:746660705
1181 1181 a, g dbSNP:775255229
1198 1198 a, c, t dbSNP:745728602
1199 1199 a, g dbSNP:778809798
1203 1203 a, c dbSNP:757200008
1207 1207 a, g dbSNP:749325083
1219 1219 c, t dbSNP:201937527
1224 1224 g, t dbSNP:201753929
1226 1226 g, t dbSNP:780620100
1244 1244 a, g dbSNP:368641715
1246 1246 c, g dbSNP:755394360
1248 1248 a, g dbSNP:151100437
1252 1252 c, g dbSNP:766922660
1253 1253 c, t dbSNP:142899386
1257 1257 c, g dbSNP:148432028
1259 1259 g, t dbSNP:763488544
1262 1262 a, c dbSNP:374451635
1263 1263 c, t dbSNP:538240247
1264 1264 c, t dbSNP:771769935
1270 1270 a, t dbSNP:759269789
1278 1278 g, t dbSNP:17847327
1286 1286 a, g dbSNP:17073229
1289 1289 a, g, t dbSNP:777804198
1296 1296 a, c dbSNP:769737512
1300 1300 c, t dbSNP:748313279
1301 1301 a, t dbSNP:781556516
1304 1304 c, t dbSNP:755267953
1305 1305 a, g dbSNP:751926904
1332 1332 a, g dbSNP:780599925
1336 1336 a, g dbSNP:758839660
1360 1360 a, g dbSNP:750874987
1364 1364 a, g dbSNP:765832081
1372 1372 a, g dbSNP:760099365
1382 1382 g, t dbSNP:752208274
1385 1385 a, g dbSNP:267600848
1427 1427 c, t dbSNP:767098050
1442 1442 c, t dbSNP:141784122
1447 1447 a, t dbSNP:1063413
1463 1463 c, t dbSNP:36120645
1475 1475 a, c dbSNP:547586757
1484 1484 a, g dbSNP:762851758
1485 1485 -, gag dbSNP:760478312
1492 1492 c, t dbSNP:773005838
1498 1498 a, g dbSNP:34652819
1500 1500 a, g dbSNP:748123821
1501 1501 c, g dbSNP:781448211
1508 1508 c, g dbSNP:375679973
1511 1511 c, g, t dbSNP:370449852
1512 1512 a, g dbSNP:758678527
1515 1515 a, g dbSNP:565181543
1518 1518 a, c dbSNP:201977118
1522 1522 c, t dbSNP:377135913
1524 1524 a, c dbSNP:752153135
1536 1536 a, g dbSNP:17847328
1539 1539 a, t dbSNP:754521531
1543 1543 c, t dbSNP:751187671
1549 1549 c, t dbSNP:766110989
1557 1557 -, c dbSNP:35411045
1566 1566 a, g dbSNP:560576837
1574 1574 a, g dbSNP:373156842
1576 1576 c, g dbSNP:142705655
1584 1584 c, t dbSNP:761877385
1597 1597 c, g dbSNP:776753534
1598 1598 c, t dbSNP:768835975
1608 1608 a, g dbSNP:372873338
1609 1609 a, g dbSNP:747145351
1626 1626 c, t dbSNP:775851406
1630 1630 c, g dbSNP:369453748
1632 1632 a, g dbSNP:746160419
1640 1640 c, t dbSNP:200072882
1648 1648 c, t dbSNP:757664610
1649 1649 c, t dbSNP:749761427
1665 1665 a, c dbSNP:778298992
1670 1670 c, t dbSNP:149291080
1671 1671 a, c, g dbSNP:765875826
1672 1672 c, t dbSNP:35263016
1682 1682 a, g dbSNP:578145229
1687 1687 c, t dbSNP:377306571
1688 1688 a, g, t dbSNP:776698636
1694 1694 a, g dbSNP:764195119
1695 1695 c, g dbSNP:141235151
1696 1696 a, c dbSNP:775653923
1697 1697 -, ccagag dbSNP:774596210
1697 1697 a, g dbSNP:772136403
1703 1703 a, g dbSNP:62427100
1704 1704 a, g dbSNP:780626452
1709 1709 c, g dbSNP:374462557
1713 1713 a, c dbSNP:774476162
1715 1715 c, t dbSNP:368929894
1719 1719 c, t dbSNP:556887561
1720 1720 c, t dbSNP:749708141
1721 1721 a, g, t dbSNP:201057231
1722 1722 a, g dbSNP:746453740
1726 1726 c, t dbSNP:779649831
1727 1727 c, t dbSNP:757889837
1733 1733 c, t dbSNP:750070946
1741 1741 c, t dbSNP:200573380
1742 1742 a, g dbSNP:17847330
1756 1756 a, t dbSNP:371698312
1768 1768 a, c dbSNP:147054773
1772 1772 c, t dbSNP:200036472
1773 1773 a, g dbSNP:752677729
1774 1774 c, t dbSNP:767601739
1784 1784 a, t dbSNP:759582013
1791 1791 c, t dbSNP:774617149
1792 1792 c, t dbSNP:771206023
1797 1797 a, g, t dbSNP:141627165
1798 1798 a, c dbSNP:770249452
1805 1805 a, c, g dbSNP:147885532
1808 1808 a, c dbSNP:771601130
1809 1809 -, c dbSNP:768840283
1810 1810 a, t dbSNP:745438962
1812 1812 a, c dbSNP:778643791
1833 1833 c, t dbSNP:756984299
1837 1837 a, g dbSNP:374631467
1847 1847 g, t dbSNP:777741438
1854 1854 a, g dbSNP:756012523
1859 1859 a, g dbSNP:752655598
1880 1880 a, t dbSNP:138688804
1892 1892 c, g dbSNP:759653376
1900 1900 a, g dbSNP:751593481
1908 1908 c, g dbSNP:766525788
1910 1910 a, g dbSNP:763180631
1918 1918 a, g dbSNP:773466984
1953 1953 a, c dbSNP:372153249
1969 1969 a, c dbSNP:762144772
1975 1975 a, c dbSNP:777194150
1978 1978 c, t dbSNP:769049757
1979 1979 a, c dbSNP:200257642
1980 1980 c, t dbSNP:774021173
1989 1989 c, g dbSNP:201957325
1991 1991 a, g dbSNP:72546304
1994 1994 c, t dbSNP:145034248
1997 1997 c, g dbSNP:755923312
1999 1999 a, c, g, t dbSNP:754880138
2000 2000 a, c, g dbSNP:145386261
2001 2001 c, g dbSNP:758526896
2003 2003 a, c, g, t dbSNP:150039945
2004 2004 a, c, g dbSNP:764612601
2005 2005 c, t dbSNP:149400255
2006 2006 c, g dbSNP:761118576
2027 2027 a, c, t dbSNP:17847331
2030 2030 c, t dbSNP:748862556
2035 2035 c, t dbSNP:772992931
2039 2039 c, t dbSNP:769332223
2043 2043 a, c dbSNP:747916100
2044 2044 a, c dbSNP:780866537
2046 2046 a, g dbSNP:751709706
2050 2050 c, t dbSNP:754901227
2056 2056 a, g dbSNP:373757589
2058 2058 a, g dbSNP:780028654
2067 2067 a, g dbSNP:758471604
2068 2068 c, t dbSNP:750509016
2075 2075 a, g dbSNP:765442934
2076 2076 -, cctgggg dbSNP:749424025
2076 2076 a, c dbSNP:757445503
2079 2079 a, g dbSNP:754192424
2084 2084 a, c dbSNP:370253986
2088 2088 c, t dbSNP:548577685
2103 2103 a, c dbSNP:776125291
2104 2104 a, g dbSNP:764191640
2110 2110 a, t dbSNP:768204588
2111 2111 a, g dbSNP:762482586
2114 2114 g, t dbSNP:772859501
2116 2116 a, g dbSNP:769435521
2122 2122 a, c dbSNP:374421447
2123 2123 g, t dbSNP:776397265
2125 2125 -, gca dbSNP:775540928
2127 2127 -, c dbSNP:35716705
2131 2131 c, t dbSNP:768326522
2142 2142 a, g dbSNP:746874822
2144 2144 a, c, g dbSNP:527350024
2160 2160 a, g dbSNP:745900352
2180 2180 c, t dbSNP:778890400
2185 2185 a, c dbSNP:36087014
2188 2188 a, g dbSNP:757399152
2198 2198 a, t dbSNP:753984271
2216 2216 c, t dbSNP:778115846
2219 2219 c, t dbSNP:756540757
2222 2222 a, g dbSNP:370394316
2228 2228 c, t dbSNP:768076125
2253 2253 a, g dbSNP:760139789
2254 2254 c, t dbSNP:749905224
2258 2258 a, t dbSNP:764618922
2264 2264 a, g dbSNP:761447530
2267 2267 a, t dbSNP:776147456
2269 2269 -, attt dbSNP:769843457
2272 2272 c, t dbSNP:560190372
2273 2273 a, g dbSNP:146546302
2283 2283 a, g dbSNP:775287768
2284 2284 g, t dbSNP:771959962
2287 2287 -, atg dbSNP:746386623
2287 2287 a, g dbSNP:745775222
2330 2330 g, t dbSNP:769371400
2338 2338 -, a dbSNP:563450219
2339 2339 c, t dbSNP:150413533
2370 2370 a, g dbSNP:3544
2385 2385 -, gat dbSNP:552001777
2387 2387 -, aga dbSNP:112027877
2421 2421 c, t dbSNP:753866564
2460 2460 a, g dbSNP:373528883
2540 2540 c, t dbSNP:766182254
2591 2591 a, g dbSNP:775132795
2614 2614 c, t dbSNP:140595869
2641 2641 a, g dbSNP:765027393
2677 2677 g, t dbSNP:759398749
2713 2713 a, g dbSNP:541432527
2731 2731 a, g dbSNP:544894567
2734 2734 a, g dbSNP:1804733
2762 2762 a, c dbSNP:151333282
2813 2813 -, t dbSNP:376801986
2857 2857 a, g dbSNP:142469019
2889 2889 c, t dbSNP:780234856
2927 2927 a, c, g dbSNP:200779763
2929