×

PLCB4 cDNA ORF clone, Homo sapiens (human)

Gene Symbol PLCB4
Entrez Gene ID 5332
Full Name phospholipase C, beta 4
Synonyms ARCND2, PI-PLC
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

20

20p12

Summary The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals in the retina. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2010]. lac of sum
Disorder MIM:

600810


mRNA and Protein(s)

mRNA Protein Name
XM_006723569 XP_006723632 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
XM_006723568 XP_006723631 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
XM_005260724 XP_005260781 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
XM_011529251 XP_011527553 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
XM_011529252 XP_011527554 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_011529253 XP_011527555 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X3
XM_011529254 XP_011527556 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X4
XM_005260728 XP_005260785 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X5
NM_000933 NP_000924 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform a
NM_182797 NP_877949 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform b
NM_001172646 NP_001166117 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform c



Homo sapiens (human) PLCB4 NP_877949.2
Pan troglodytes (chimpanzee) PLCB4 XP_001168348.2
Macaca mulatta (Rhesus monkey) PLCB4 XP_002798260.1
Canis lupus familiaris (dog) PLCB4 XP_859719.2
Bos taurus (cattle) PLCB4 NP_001159982.1
Mus musculus (house mouse) Plcb4 NP_038857.1
Rattus norvegicus (Norway rat) Plcb4 NP_077329.1
Gallus gallus (chicken) PLCB4 NP_001186364.1
Danio rerio (zebrafish) LOC100537538 XP_003200348.2
Drosophila melanogaster (fruit fly) norpA NP_001014720.1
Caenorhabditis elegans egl-8 NP_001021346.1
Xenopus (Silurana) tropicalis (western clawed frog) plcb4 XP_004914780.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PLCB4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCB4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu52719 XM_006723569 PREDICTED: Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu52719 XM_006723568 PREDICTED: Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu52719 XM_005260724 PREDICTED: Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu52719 XM_011529251 PREDICTED: Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu14145 XM_011529252 PREDICTED: Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu59762 XM_011529253 PREDICTED: Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu59763 XM_011529254 PREDICTED: Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu52720 XM_005260728 PREDICTED: Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu14145 NM_000933 Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu14162 NM_182797 Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu18159 NM_001172646 Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $839.30
$1199.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu52719
Clone ID Related Accession (Same CDS sequence) XM_005260724 , XM_006723568 , XM_006723569 , XM_011529251
Accession Version XM_006723569.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011387.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006723632.1
CDS353..3973
Misc Feature(1)401..781(+)
Misc Feature(2)416..703(+)
Misc Feature(3)425..511(+)
Misc Feature(4)1004..2809(+)
Misc Feature(5)1013..1288(+)
Misc Feature(6)1286..2392(+)
Misc Feature(7)1334..2257(+)
Misc Feature(8)1334..1477(+)
Misc Feature(9)1337..1579(+)
Misc Feature(10)2492..2842(+)
Misc Feature(11)2543..2734(+)
Misc Feature(12)3113..3253(+)
Translation

Target ORF information:

RefSeq Version XM_006723569
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006723569

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52719
Clone ID Related Accession (Same CDS sequence) XM_005260724 , XM_006723568 , XM_006723569 , XM_011529251
Accession Version XM_006723568.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011387.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578835416. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006723631.1
CDS255..3875
Misc Feature(1)303..683(+)
Misc Feature(2)318..605(+)
Misc Feature(3)327..413(+)
Misc Feature(4)906..2711(+)
Misc Feature(5)915..1190(+)
Misc Feature(6)1188..2294(+)
Misc Feature(7)1236..2159(+)
Misc Feature(8)1236..1379(+)
Misc Feature(9)1239..1481(+)
Misc Feature(10)2394..2744(+)
Misc Feature(11)2445..2636(+)
Misc Feature(12)3015..3155(+)
Translation

Target ORF information:

RefSeq Version XM_006723568
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006723568

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52719
Clone ID Related Accession (Same CDS sequence) XM_005260724 , XM_006723568 , XM_006723569 , XM_011529251
Accession Version XM_005260724.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011387.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005260781.1
CDS273..3893
Misc Feature(1)321..701(+)
Misc Feature(2)336..623(+)
Misc Feature(3)345..431(+)
Misc Feature(4)924..2729(+)
Misc Feature(5)933..1208(+)
Misc Feature(6)1206..2312(+)
Misc Feature(7)1254..2177(+)
Misc Feature(8)1254..1397(+)
Misc Feature(9)1257..1499(+)
Misc Feature(10)2412..2762(+)
Misc Feature(11)2463..2654(+)
Misc Feature(12)3033..3173(+)
Translation

Target ORF information:

RefSeq Version XM_005260724
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005260724

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52719
Clone ID Related Accession (Same CDS sequence) XM_005260724 , XM_006723568 , XM_006723569 , XM_011529251
Accession Version XM_011529251.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011387.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011527553.1
CDS141..3761
Misc Feature(1)189..569(+)
Misc Feature(2)204..491(+)
Misc Feature(3)213..299(+)
Misc Feature(4)792..2597(+)
Misc Feature(5)801..1076(+)
Misc Feature(6)1074..2180(+)
Misc Feature(7)1122..2045(+)
Misc Feature(8)1122..1265(+)
Misc Feature(9)1125..1367(+)
Misc Feature(10)2280..2630(+)
Misc Feature(11)2331..2522(+)
Misc Feature(12)2901..3041(+)
Translation

Target ORF information:

RefSeq Version XM_011529251
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529251

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14145
Clone ID Related Accession (Same CDS sequence) NM_000933 , XM_011529252
Accession Version XM_011529252.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011387.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGGCCTGGTC
ACTGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTAGGTGC TACCACTAAT
ATCCATCCAT ATTTGTCCAC AATGATCAAC TACGCCCAGC CTGTAAAGTT TCAAGGTTTC
CATGTGGCAG AAGAACGCAA TATTCATTAT AACATGTCTT CTTTTAATGA ATCAGTCGGT
CTTGGCTACT TGAAGACACA TGCAATTGAA TTTGTCAATT ATAACAAACG GCAAATGAGT
CGCATTTACC CCAAGGGAGG CCGAGTCGAT TCCAGTAATT ACATGCCTCA GATTTTCTGG
AACGCTGGCT GCCAGATGGT TTCACTGAAC TATCAAACCC CAGATTTAGC GATGCAATTG
AATCAGGGAA AATTTGAGTA TAATGGATCG TGCGGGTACC TTCTCAAACC AGATTTCATG
AGGCGGCCTG ATCGAACATT TGACCCCTTC TCTGAAACTC CTGTTGATGG TGTTATTGCA
GCCACTTGCT CAGTGCAGGT TATATCAGGT CAATTCTTAT CAGATAAGAA AATTGGCACC
TACGTAGAGG TGGATATGTA TGGGTTGCCC ACTGACACCA TACGTAAGGA ATTCCGAACT
CGCATGGTTA TGAATAATGG ACTCAATCCA GTTTACAATG AAGAGTCATT TGTATTTCGG
AAGGTGATCC TGCCGGACCT GGCTGTCTTG AGAATAGCTG TGTATGATGA TAACAACAAG
CTGATTGGCC AGAGGATCCT CCCGCTTGAT GGCCTCCAAG CCGGATATCG ACACATTTCC
CTTCGAAATG AGGGAAATAA ACCATTATCA CTACCAACAA TTTTCTGCAA TATTGTTCTT
AAAACATATG TGCCTGATGG ATTTGGAGAT ATCGTGGATG CTTTATCAGA TCCAAAGAAA
TTTCTCTCAA TTACAGAAAA GAGAGCAGAC CAAATGAGAG CTATGGGCAT TGAAACTAGT
GACATAGCCG ACGTGCCCAG TGACACTTCC AAAAATGACA AGAAAGGAAA GGCCAACACC
GCCAAAGCAA ATGTGACCCC TCAGAGTAGC TCTGAGCTCA GACCAACCAC CACGGCTGCC
CTGGCCTCTG GTGTGGAAGC CAAGAAAGGT ATTGAACTTA TCCCTCAAGT AAGGATAGAA
GACTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAGCAGAA GGAGCTAAAT
TCTTTAAAGA AGAAACATGC AAAGGAACAC AGTACCATGC AGAAGTTACA CTGCACGCAA
GTTGACAAAA TTGTGGCACA GTATGACAAA GAGAAGTCGA CTCATGAGAA AATCCTAGAG
AAGGCAATGA AGAAGAAGGG GGGAAGTAAT TGTCTCGAAA TGAAAAAAGA AACAGAAATC
AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AAGAGATTGT AGCACAGCAC
ACAAAGGAAT GGTCAGAAAT GATCAATACC CACAGTGCTG AGGAGCAAGA AATCCGAGAC
CTGCACCTCA GCCAGCAGTG TGAGCTGCTG AAAAAGCTAC TCATCAATGC CCACGAGCAG
CAAACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AGGAAATGCG AGCACACCAG
GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCTAT CAAGAATAAA
GCAGAACGGG AAAGGCGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
GAAAGAAAGA GACTTGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
CTTGAACATC TAGAATTCCT AGAGAAACAG AATGAGCAGC TTTTGAAATC CTGTCATGCA
GTGTCCCAAA CGCAAGGCGA AGGAGATGCA GCAGATGGTG AAATTGGAAG CCGAGATGGA
CCGCAGACCA GCAACAGTAG TATGAAACTC CAAAATGCAA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011527554.1
CDS141..3725
Misc Feature(1)189..569(+)
Misc Feature(2)204..491(+)
Misc Feature(3)213..299(+)
Misc Feature(4)792..2561(+)
Misc Feature(5)801..1076(+)
Misc Feature(6)1074..2144(+)
Misc Feature(7)1122..2009(+)
Misc Feature(8)1122..1265(+)
Misc Feature(9)1125..1367(+)
Misc Feature(10)2244..2594(+)
Misc Feature(11)2295..2486(+)
Misc Feature(12)2865..3005(+)
Translation

Target ORF information:

RefSeq Version XM_011529252
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529252

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGGCCTGGTC
ACTGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTAGGTGC TACCACTAAT
ATCCATCCAT ATTTGTCCAC AATGATCAAC TACGCCCAGC CTGTAAAGTT TCAAGGTTTC
CATGTGGCAG AAGAACGCAA TATTCATTAT AACATGTCTT CTTTTAATGA ATCAGTCGGT
CTTGGCTACT TGAAGACACA TGCAATTGAA TTTGTCAATT ATAACAAACG GCAAATGAGT
CGCATTTACC CCAAGGGAGG CCGAGTCGAT TCCAGTAATT ACATGCCTCA GATTTTCTGG
AACGCTGGCT GCCAGATGGT TTCACTGAAC TATCAAACCC CAGATTTAGC GATGCAATTG
AATCAGGGAA AATTTGAGTA TAATGGATCG TGCGGGTACC TTCTCAAACC AGATTTCATG
AGGCGGCCTG ATCGAACATT TGACCCCTTC TCTGAAACTC CTGTTGATGG TGTTATTGCA
GCCACTTGCT CAGTGCAGGT TATATCAGGT CAATTCTTAT CAGATAAGAA AATTGGCACC
TACGTAGAGG TGGATATGTA TGGGTTGCCC ACTGACACCA TACGTAAGGA ATTCCGAACT
CGCATGGTTA TGAATAATGG ACTCAATCCA GTTTACAATG AAGAGTCATT TGTATTTCGG
AAGGTGATCC TGCCGGACCT GGCTGTCTTG AGAATAGCTG TGTATGATGA TAACAACAAG
CTGATTGGCC AGAGGATCCT CCCGCTTGAT GGCCTCCAAG CCGGATATCG ACACATTTCC
CTTCGAAATG AGGGAAATAA ACCATTATCA CTACCAACAA TTTTCTGCAA TATTGTTCTT
AAAACATATG TGCCTGATGG ATTTGGAGAT ATCGTGGATG CTTTATCAGA TCCAAAGAAA
TTTCTCTCAA TTACAGAAAA GAGAGCAGAC CAAATGAGAG CTATGGGCAT TGAAACTAGT
GACATAGCCG ACGTGCCCAG TGACACTTCC AAAAATGACA AGAAAGGAAA GGCCAACACC
GCCAAAGCAA ATGTGACCCC TCAGAGTAGC TCTGAGCTCA GACCAACCAC CACGGCTGCC
CTGGCCTCTG GTGTGGAAGC CAAGAAAGGT ATTGAACTTA TCCCTCAAGT AAGGATAGAA
GACTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAGCAGAA GGAGCTAAAT
TCTTTAAAGA AGAAACATGC AAAGGAACAC AGTACCATGC AGAAGTTACA CTGCACGCAA
GTTGACAAAA TTGTGGCACA GTATGACAAA GAGAAGTCGA CTCATGAGAA AATCCTAGAG
AAGGCAATGA AGAAGAAGGG GGGAAGTAAT TGTCTCGAAA TGAAAAAAGA AACAGAAATC
AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AAGAGATTGT AGCACAGCAC
ACAAAGGAAT GGTCAGAAAT GATCAATACC CACAGTGCTG AGGAGCAAGA AATCCGAGAC
CTGCACCTCA GCCAGCAGTG TGAGCTGCTG AAAAAGCTAC TCATCAATGC CCACGAGCAG
CAAACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AGGAAATGCG AGCACACCAG
GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCTAT CAAGAATAAA
GCAGAACGGG AAAGGCGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
GAAAGAAAGA GACTTGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
CTTGAACATC TAGAATTCCT AGAGAAACAG AATGAGCAGC TTTTGAAATC CTGTCATGCA
GTGTCCCAAA CGCAAGGCGA AGGAGATGCA GCAGATGGTG AAATTGGAAG CCGAGATGGA
CCGCAGACCA GCAACAGTAG TATGAAACTC CAAAATGCAA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59762
Clone ID Related Accession (Same CDS sequence) XM_011529253
Accession Version XM_011529253.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3354bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011387.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011527555.1
CDS16..3369
Misc Feature(1)64..444(+)
Misc Feature(2)79..366(+)
Misc Feature(3)88..174(+)
Misc Feature(4)667..2472(+)
Misc Feature(5)676..951(+)
Misc Feature(6)949..2055(+)
Misc Feature(7)997..1920(+)
Misc Feature(8)997..1140(+)
Misc Feature(9)1000..1242(+)
Misc Feature(10)2155..2505(+)
Misc Feature(11)2206..2397(+)
Misc Feature(12)2776..2916(+)
Misc Feature(13)2848..3234(+)
Translation

Target ORF information:

RefSeq Version XM_011529253
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529253

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59763
Clone ID Related Accession (Same CDS sequence) XM_011529254
Accession Version XM_011529254.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3141bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011387.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGAAAAACA TCTGCAATGA GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG 
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011527556.1
CDS3855..6995
Misc Feature(1)4026..5831(+)
Misc Feature(2)4035..4310(+)
Misc Feature(3)4308..5414(+)
Misc Feature(4)4356..5279(+)
Misc Feature(5)4356..4499(+)
Misc Feature(6)4359..4601(+)
Misc Feature(7)5514..5864(+)
Misc Feature(8)5565..5756(+)
Misc Feature(9)6135..6275(+)
Translation

Target ORF information:

RefSeq Version XM_011529254
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529254

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGAAAAACA TCTGCAATGA GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG 
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52720
Clone ID Related Accession (Same CDS sequence) XM_005260728
Accession Version XM_005260728.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2817bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011387.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGCAGATCA TTGAGATGTA TGAACCTGAT GAAGATTTGA AGAAAAAAGG CCTTATATCA 
AGTGATGGGT TTTGCAGATA TCTGATGTCA GATGAAAACG CCCCAGTCTT CCTAGATCGT
TTAGAACTTT ACCAAGAAAT GGACCATCCT CTGGCTCACT ACTTCATCAG TTCTTCCCAT
AACACTTATC TCACTGGCAG ACAGTTCGGC GGGAAGTCTT CGGTAGAAAT GTACAGACAG
GTTCTCCTGG CTGGTTGCAG ATGTGTTGAA CTTGACTGCT GGGATGGAAA AGGTGAAGAC
CAAGAACCAA TAATAACTCA TGGAAAAGCA ATGTGTACAG ATATCCTTTT TAAGGATGTA
ATTCAAGCCA TCAAGGAAAC TGCATTTGTC ACATCAGAAT ATCCTGTAAT TCTCTCCTTT
GAAAATCACT GCAGCAAATA TCAACAGTAC AAGATGTCCA AATATTGCGA AGATCTATTT
GGGGATCTCC TGTTGAAACA AGCACTTGAA TCACATCCAC TTGAACCAGG CAGGGCTTTG
CCATCCCCCA ATGACCTCAA AAGAAAAATA CTCATAAAAA ACAAGCGGCT GAAACCTGAA
GTTGAAAAAA AACAGCTGGA AGCTTTGAGA AGCATGATGG AAGCTGGAGA ATCTGCCTCC
CCAGCAAACA TCTTAGAGGA CGATAATGAA GAGGAGATCG AAAGTGCTGA CCAAGAGGAG
GAAGCTCACC CCGAATTCAA ATTTGGAAAT GAACTTTCTG CTGATGACTT GGGTCACAAG
GAAGCTGTTG CAAATAGCGT CAAGAAGGCT TCAGATGACC TTGAACATGA AAACAACAAA
AAGGGCCTGG TCACTGTAGA AGATGAGCAG GCGTGGATGG CATCTTATAA ATATGTAGGT
GCTACCACTA ATATCCATCC ATATTTGTCC ACAATGATCA ACTACGCCCA GCCTGTAAAG
TTTCAAGGTT TCCATGTGGC AGAAGAACGC AATATTCATT ATAACATGTC TTCTTTTAAT
GAATCAGTCG GTCTTGGCTA CTTGAAGACA CATGCAATTG AATTTGTCAA TTATAACAAA
CGGCAAATGA GTCGCATTTA CCCCAAGGGA GGCCGAGTCG ATTCCAGTAA TTACATGCCT
CAGATTTTCT GGAACGCTGG CTGCCAGATG GTTTCACTGA ACTATCAAAC CCCAGATTTA
GCGATGCAAT TGAATCAGGG AAAATTTGAG TATAATGGAT CGTGCGGGTA CCTTCTCAAA
CCAGATTTCA TGAGGCGGCC TGATCGAACA TTTGACCCCT TCTCTGAAAC TCCTGTTGAT
GGTGTTATTG CAGCCACTTG CTCAGTGCAG GTTATATCAG GTCAATTCTT ATCAGATAAG
AAAATTGGCA CCTACGTAGA GGTGGATATG TATGGGTTGC CCACTGACAC CATACGTAAG
GAATTCCGAA CTCGCATGGT TATGAATAAT GGACTCAATC CAGTTTACAA TGAAGAGTCA
TTTGTATTTC GGAAGGTGAT CCTGCCGGAC CTGGCTGTCT TGAGAATAGC TGTGTATGAT
GATAACAACA AGCTGATTGG CCAGAGGATC CTCCCGCTTG ATGGCCTCCA AGCCGGATAT
CGACACATTT CCCTTCGAAA TGAGGGAAAT AAACCATTAT CACTACCAAC AATTTTCTGC
AATATTGTTC TTAAAACATA TGTGCCTGAT GGATTTGGAG ATATCGTGGA TGCTTTATCA
GATCCAAAGA AATTTCTCTC AATTACAGAA AAGAGAGCAG ACCAAATGAG AGCTATGGGC
ATTGAAACTA GTGACATAGC CGACGTGCCC AGTGACACTT CCAAAAATGA CAAGAAAGGA
AAGGCCAACA CCGCCAAAGC AAATGTGACC CCTCAGAGTA GCTCTGAGCT CAGACCAACC
ACCACGGCTG CCCTGGCCTC TGGTGTGGAA GCCAAGAAAG GTATTGAACT TATCCCTCAA
GTAAGGATAG AAGACTTAAA GCAGATGAAG GCTTACTTGA AGCATTTAAA GAAACAGCAG
AAGGAGCTAA ATTCTTTAAA GAAGAAACAT GCAAAGGAAC ACAGTACCAT GCAGAAGTTA
CACTGCACGC AAGTTGACAA AATTGTGGCA CAGTATGACA AAGAGAAGTC GACTCATGAG
AAAATCCTAG AGAAGGCAAT GAAGAAGAAG GGGGGAAGTA ATTGTCTCGA AATGAAAAAA
GAAACAGAAA TCAAAATTCA GACGCTGACA TCAGATCACA AATCTAAGGT CAAAGAGATT
GTAGCACAGC ACACAAAGGA ATGGTCAGAA ATGATCAATA CCCACAGTGC TGAGGAGCAA
GAAATCCGAG ACCTGCACCT CAGCCAGCAG TGTGAGCTGC TGAAAAAGCT ACTCATCAAT
GCCCACGAGC AGCAAACCCA GCAGCTGAAA CTGTCCCATG ACAGGGAAAG CAAGGAAATG
CGAGCACACC AGGCTAAGAT TTCTATGGAA AATAGCAAAG CCATCAGCCA AGATAAATCT
ATCAAGAATA AAGCAGAACG GGAAAGGCGA GTCAGGGAGT TAAACAGCAG CAACACTAAA
AAGTTTCTGG AAGAAAGAAA GAGACTTGCC ATGAAGCAGT CCAAAGAAAT GGATCAGTTG
AAAAAAGTCC AGCTTGAACA TCTAGAATTC CTAGAGAAAC AGAATGAGCA GCTTTTGAAA
TCCTGTCATG CAGTGTCCCA AACGCAAGGC GAAGGAGATG CAGCAGATGG TGAAATTGGA
AGCCGAGATG GACCGCAGAC CAGCAACAGT AGTATGAAAC TCCAAAATGC AAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005260785.1
CDS244..3060
Misc Feature(1)244..375(+)
Misc Feature(2)373..1479(+)
Misc Feature(3)421..1344(+)
Misc Feature(4)421..564(+)
Misc Feature(5)424..666(+)
Misc Feature(6)1579..1929(+)
Misc Feature(7)1630..1821(+)
Misc Feature(8)2200..2340(+)
Translation

Target ORF information:

RefSeq Version XM_005260728
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005260728

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGCAGATCA TTGAGATGTA TGAACCTGAT GAAGATTTGA AGAAAAAAGG CCTTATATCA 
AGTGATGGGT TTTGCAGATA TCTGATGTCA GATGAAAACG CCCCAGTCTT CCTAGATCGT
TTAGAACTTT ACCAAGAAAT GGACCATCCT CTGGCTCACT ACTTCATCAG TTCTTCCCAT
AACACTTATC TCACTGGCAG ACAGTTCGGC GGGAAGTCTT CGGTAGAAAT GTACAGACAG
GTTCTCCTGG CTGGTTGCAG ATGTGTTGAA CTTGACTGCT GGGATGGAAA AGGTGAAGAC
CAAGAACCAA TAATAACTCA TGGAAAAGCA ATGTGTACAG ATATCCTTTT TAAGGATGTA
ATTCAAGCCA TCAAGGAAAC TGCATTTGTC ACATCAGAAT ATCCTGTAAT TCTCTCCTTT
GAAAATCACT GCAGCAAATA TCAACAGTAC AAGATGTCCA AATATTGCGA AGATCTATTT
GGGGATCTCC TGTTGAAACA AGCACTTGAA TCACATCCAC TTGAACCAGG CAGGGCTTTG
CCATCCCCCA ATGACCTCAA AAGAAAAATA CTCATAAAAA ACAAGCGGCT GAAACCTGAA
GTTGAAAAAA AACAGCTGGA AGCTTTGAGA AGCATGATGG AAGCTGGAGA ATCTGCCTCC
CCAGCAAACA TCTTAGAGGA CGATAATGAA GAGGAGATCG AAAGTGCTGA CCAAGAGGAG
GAAGCTCACC CCGAATTCAA ATTTGGAAAT GAACTTTCTG CTGATGACTT GGGTCACAAG
GAAGCTGTTG CAAATAGCGT CAAGAAGGCT TCAGATGACC TTGAACATGA AAACAACAAA
AAGGGCCTGG TCACTGTAGA AGATGAGCAG GCGTGGATGG CATCTTATAA ATATGTAGGT
GCTACCACTA ATATCCATCC ATATTTGTCC ACAATGATCA ACTACGCCCA GCCTGTAAAG
TTTCAAGGTT TCCATGTGGC AGAAGAACGC AATATTCATT ATAACATGTC TTCTTTTAAT
GAATCAGTCG GTCTTGGCTA CTTGAAGACA CATGCAATTG AATTTGTCAA TTATAACAAA
CGGCAAATGA GTCGCATTTA CCCCAAGGGA GGCCGAGTCG ATTCCAGTAA TTACATGCCT
CAGATTTTCT GGAACGCTGG CTGCCAGATG GTTTCACTGA ACTATCAAAC CCCAGATTTA
GCGATGCAAT TGAATCAGGG AAAATTTGAG TATAATGGAT CGTGCGGGTA CCTTCTCAAA
CCAGATTTCA TGAGGCGGCC TGATCGAACA TTTGACCCCT TCTCTGAAAC TCCTGTTGAT
GGTGTTATTG CAGCCACTTG CTCAGTGCAG GTTATATCAG GTCAATTCTT ATCAGATAAG
AAAATTGGCA CCTACGTAGA GGTGGATATG TATGGGTTGC CCACTGACAC CATACGTAAG
GAATTCCGAA CTCGCATGGT TATGAATAAT GGACTCAATC CAGTTTACAA TGAAGAGTCA
TTTGTATTTC GGAAGGTGAT CCTGCCGGAC CTGGCTGTCT TGAGAATAGC TGTGTATGAT
GATAACAACA AGCTGATTGG CCAGAGGATC CTCCCGCTTG ATGGCCTCCA AGCCGGATAT
CGACACATTT CCCTTCGAAA TGAGGGAAAT AAACCATTAT CACTACCAAC AATTTTCTGC
AATATTGTTC TTAAAACATA TGTGCCTGAT GGATTTGGAG ATATCGTGGA TGCTTTATCA
GATCCAAAGA AATTTCTCTC AATTACAGAA AAGAGAGCAG ACCAAATGAG AGCTATGGGC
ATTGAAACTA GTGACATAGC CGACGTGCCC AGTGACACTT CCAAAAATGA CAAGAAAGGA
AAGGCCAACA CCGCCAAAGC AAATGTGACC CCTCAGAGTA GCTCTGAGCT CAGACCAACC
ACCACGGCTG CCCTGGCCTC TGGTGTGGAA GCCAAGAAAG GTATTGAACT TATCCCTCAA
GTAAGGATAG AAGACTTAAA GCAGATGAAG GCTTACTTGA AGCATTTAAA GAAACAGCAG
AAGGAGCTAA ATTCTTTAAA GAAGAAACAT GCAAAGGAAC ACAGTACCAT GCAGAAGTTA
CACTGCACGC AAGTTGACAA AATTGTGGCA CAGTATGACA AAGAGAAGTC GACTCATGAG
AAAATCCTAG AGAAGGCAAT GAAGAAGAAG GGGGGAAGTA ATTGTCTCGA AATGAAAAAA
GAAACAGAAA TCAAAATTCA GACGCTGACA TCAGATCACA AATCTAAGGT CAAAGAGATT
GTAGCACAGC ACACAAAGGA ATGGTCAGAA ATGATCAATA CCCACAGTGC TGAGGAGCAA
GAAATCCGAG ACCTGCACCT CAGCCAGCAG TGTGAGCTGC TGAAAAAGCT ACTCATCAAT
GCCCACGAGC AGCAAACCCA GCAGCTGAAA CTGTCCCATG ACAGGGAAAG CAAGGAAATG
CGAGCACACC AGGCTAAGAT TTCTATGGAA AATAGCAAAG CCATCAGCCA AGATAAATCT
ATCAAGAATA AAGCAGAACG GGAAAGGCGA GTCAGGGAGT TAAACAGCAG CAACACTAAA
AAGTTTCTGG AAGAAAGAAA GAGACTTGCC ATGAAGCAGT CCAAAGAAAT GGATCAGTTG
AAAAAAGTCC AGCTTGAACA TCTAGAATTC CTAGAGAAAC AGAATGAGCA GCTTTTGAAA
TCCTGTCATG CAGTGTCCCA AACGCAAGGC GAAGGAGATG CAGCAGATGG TGAAATTGGA
AGCCGAGATG GACCGCAGAC CAGCAACAGT AGTATGAAAC TCCAAAATGC AAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14145
Clone ID Related Accession (Same CDS sequence) NM_000933 , XM_011529252
Accession Version NM_000933.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB270238.1, BX648680.1, BC117458.1, DR761451.1 and AL117633.1. On Feb 24, 2010 this sequence version replaced gi:33469932. Transcript Variant: This variant (1) lacks an alternate in-frame exon in the central coding region and includes an alternate exon in the 3' coding region that results in a frameshift, compared to variant 3. The complete exon combination of the 5' UTR for this variant has not been determined. The resulting isoform (a) lacks an internal segment and has a longer and distinct C-terminus, compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGGCCTGGTC
ACTGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTAGGTGC TACCACTAAT
ATCCATCCAT ATTTGTCCAC AATGATCAAC TACGCCCAGC CTGTAAAGTT TCAAGGTTTC
CATGTGGCAG AAGAACGCAA TATTCATTAT AACATGTCTT CTTTTAATGA ATCAGTCGGT
CTTGGCTACT TGAAGACACA TGCAATTGAA TTTGTCAATT ATAACAAACG GCAAATGAGT
CGCATTTACC CCAAGGGAGG CCGAGTCGAT TCCAGTAATT ACATGCCTCA GATTTTCTGG
AACGCTGGCT GCCAGATGGT TTCACTGAAC TATCAAACCC CAGATTTAGC GATGCAATTG
AATCAGGGAA AATTTGAGTA TAATGGATCG TGCGGGTACC TTCTCAAACC AGATTTCATG
AGGCGGCCTG ATCGAACATT TGACCCCTTC TCTGAAACTC CTGTTGATGG TGTTATTGCA
GCCACTTGCT CAGTGCAGGT TATATCAGGT CAATTCTTAT CAGATAAGAA AATTGGCACC
TACGTAGAGG TGGATATGTA TGGGTTGCCC ACTGACACCA TACGTAAGGA ATTCCGAACT
CGCATGGTTA TGAATAATGG ACTCAATCCA GTTTACAATG AAGAGTCATT TGTATTTCGG
AAGGTGATCC TGCCGGACCT GGCTGTCTTG AGAATAGCTG TGTATGATGA TAACAACAAG
CTGATTGGCC AGAGGATCCT CCCGCTTGAT GGCCTCCAAG CCGGATATCG ACACATTTCC
CTTCGAAATG AGGGAAATAA ACCATTATCA CTACCAACAA TTTTCTGCAA TATTGTTCTT
AAAACATATG TGCCTGATGG ATTTGGAGAT ATCGTGGATG CTTTATCAGA TCCAAAGAAA
TTTCTCTCAA TTACAGAAAA GAGAGCAGAC CAAATGAGAG CTATGGGCAT TGAAACTAGT
GACATAGCCG ACGTGCCCAG TGACACTTCC AAAAATGACA AGAAAGGAAA GGCCAACACC
GCCAAAGCAA ATGTGACCCC TCAGAGTAGC TCTGAGCTCA GACCAACCAC CACGGCTGCC
CTGGCCTCTG GTGTGGAAGC CAAGAAAGGT ATTGAACTTA TCCCTCAAGT AAGGATAGAA
GACTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAGCAGAA GGAGCTAAAT
TCTTTAAAGA AGAAACATGC AAAGGAACAC AGTACCATGC AGAAGTTACA CTGCACGCAA
GTTGACAAAA TTGTGGCACA GTATGACAAA GAGAAGTCGA CTCATGAGAA AATCCTAGAG
AAGGCAATGA AGAAGAAGGG GGGAAGTAAT TGTCTCGAAA TGAAAAAAGA AACAGAAATC
AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AAGAGATTGT AGCACAGCAC
ACAAAGGAAT GGTCAGAAAT GATCAATACC CACAGTGCTG AGGAGCAAGA AATCCGAGAC
CTGCACCTCA GCCAGCAGTG TGAGCTGCTG AAAAAGCTAC TCATCAATGC CCACGAGCAG
CAAACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AGGAAATGCG AGCACACCAG
GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCTAT CAAGAATAAA
GCAGAACGGG AAAGGCGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
GAAAGAAAGA GACTTGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
CTTGAACATC TAGAATTCCT AGAGAAACAG AATGAGCAGC TTTTGAAATC CTGTCATGCA
GTGTCCCAAA CGCAAGGCGA AGGAGATGCA GCAGATGGTG AAATTGGAAG CCGAGATGGA
CCGCAGACCA GCAACAGTAG TATGAAACTC CAAAATGCAA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000924.3
CDS16..3600
Misc Feature(1)19..21(+)
Misc Feature(2)64..444(+)
Misc Feature(3)79..366(+)
Misc Feature(4)88..174(+)
Misc Feature(5)667..2436(+)
Misc Feature(6)676..951(+)
Misc Feature(7)949..2019(+)
Misc Feature(8)997..1884(+)
Misc Feature(9)997..1140(+)
Misc Feature(10)1000..1242(+)
Misc Feature(11)2119..2469(+)
Misc Feature(12)2170..2361(+)
Misc Feature(13)2671..2673(+)
Misc Feature(14)2740..2880(+)
Exon (1)1..99
Gene:PLCB4
Gene Synonym:
Exon (2)100..180
Gene:PLCB4
Gene Synonym:
Exon (3)181..240
Gene:PLCB4
Gene Synonym:
Exon (4)241..384
Gene:PLCB4
Gene Synonym:
Exon (5)385..464
Gene:PLCB4
Gene Synonym:
Exon (6)465..518
Gene:PLCB4
Gene Synonym:
Exon (7)519..600
Gene:PLCB4
Gene Synonym:
Exon (8)601..701
Gene:PLCB4
Gene Synonym:
Exon (9)702..759
Gene:PLCB4
Gene Synonym:
Exon (10)760..868
Gene:PLCB4
Gene Synonym:
Exon (11)869..1079
Gene:PLCB4
Gene Synonym:
Exon (12)1080..1173
Gene:PLCB4
Gene Synonym:
Exon (13)1174..1253
Gene:PLCB4
Gene Synonym:
Exon (14)1254..1338
Gene:PLCB4
Gene Synonym:
Exon (15)1339..1429
Gene:PLCB4
Gene Synonym:
Exon (16)1430..1525
Gene:PLCB4
Gene Synonym:
Exon (17)1526..1626
Gene:PLCB4
Gene Synonym:
Exon (18)1627..1768
Gene:PLCB4
Gene Synonym:
Exon (19)1769..1853
Gene:PLCB4
Gene Synonym:
Exon (20)1854..1978
Gene:PLCB4
Gene Synonym:
Exon (21)1979..2030
Gene:PLCB4
Gene Synonym:
Exon (22)2031..2133
Gene:PLCB4
Gene Synonym:
Exon (23)2134..2298
Gene:PLCB4
Gene Synonym:
Exon (24)2299..2503
Gene:PLCB4
Gene Synonym:
Exon (25)2504..2592
Gene:PLCB4
Gene Synonym:
Exon (26)2593..2743
Gene:PLCB4
Gene Synonym:
Exon (27)2744..2793
Gene:PLCB4
Gene Synonym:
Exon (28)2794..2859
Gene:PLCB4
Gene Synonym:
Exon (29)2860..2975
Gene:PLCB4
Gene Synonym:
Exon (30)2976..3051
Gene:PLCB4
Gene Synonym:
Exon (31)3052..3227
Gene:PLCB4
Gene Synonym:
Exon (32)3228..3329
Gene:PLCB4
Gene Synonym:
Exon (33)3330..3387
Gene:PLCB4
Gene Synonym:
Exon (34)3388..3474
Gene:PLCB4
Gene Synonym:
Exon (35)3475..3511
Gene:PLCB4
Gene Synonym:
Exon (36)3512..5407
Gene:PLCB4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000933
Organism Homo sapiens (human)
Definition Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000933

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGGCCTGGTC
ACTGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTAGGTGC TACCACTAAT
ATCCATCCAT ATTTGTCCAC AATGATCAAC TACGCCCAGC CTGTAAAGTT TCAAGGTTTC
CATGTGGCAG AAGAACGCAA TATTCATTAT AACATGTCTT CTTTTAATGA ATCAGTCGGT
CTTGGCTACT TGAAGACACA TGCAATTGAA TTTGTCAATT ATAACAAACG GCAAATGAGT
CGCATTTACC CCAAGGGAGG CCGAGTCGAT TCCAGTAATT ACATGCCTCA GATTTTCTGG
AACGCTGGCT GCCAGATGGT TTCACTGAAC TATCAAACCC CAGATTTAGC GATGCAATTG
AATCAGGGAA AATTTGAGTA TAATGGATCG TGCGGGTACC TTCTCAAACC AGATTTCATG
AGGCGGCCTG ATCGAACATT TGACCCCTTC TCTGAAACTC CTGTTGATGG TGTTATTGCA
GCCACTTGCT CAGTGCAGGT TATATCAGGT CAATTCTTAT CAGATAAGAA AATTGGCACC
TACGTAGAGG TGGATATGTA TGGGTTGCCC ACTGACACCA TACGTAAGGA ATTCCGAACT
CGCATGGTTA TGAATAATGG ACTCAATCCA GTTTACAATG AAGAGTCATT TGTATTTCGG
AAGGTGATCC TGCCGGACCT GGCTGTCTTG AGAATAGCTG TGTATGATGA TAACAACAAG
CTGATTGGCC AGAGGATCCT CCCGCTTGAT GGCCTCCAAG CCGGATATCG ACACATTTCC
CTTCGAAATG AGGGAAATAA ACCATTATCA CTACCAACAA TTTTCTGCAA TATTGTTCTT
AAAACATATG TGCCTGATGG ATTTGGAGAT ATCGTGGATG CTTTATCAGA TCCAAAGAAA
TTTCTCTCAA TTACAGAAAA GAGAGCAGAC CAAATGAGAG CTATGGGCAT TGAAACTAGT
GACATAGCCG ACGTGCCCAG TGACACTTCC AAAAATGACA AGAAAGGAAA GGCCAACACC
GCCAAAGCAA ATGTGACCCC TCAGAGTAGC TCTGAGCTCA GACCAACCAC CACGGCTGCC
CTGGCCTCTG GTGTGGAAGC CAAGAAAGGT ATTGAACTTA TCCCTCAAGT AAGGATAGAA
GACTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAGCAGAA GGAGCTAAAT
TCTTTAAAGA AGAAACATGC AAAGGAACAC AGTACCATGC AGAAGTTACA CTGCACGCAA
GTTGACAAAA TTGTGGCACA GTATGACAAA GAGAAGTCGA CTCATGAGAA AATCCTAGAG
AAGGCAATGA AGAAGAAGGG GGGAAGTAAT TGTCTCGAAA TGAAAAAAGA AACAGAAATC
AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AAGAGATTGT AGCACAGCAC
ACAAAGGAAT GGTCAGAAAT GATCAATACC CACAGTGCTG AGGAGCAAGA AATCCGAGAC
CTGCACCTCA GCCAGCAGTG TGAGCTGCTG AAAAAGCTAC TCATCAATGC CCACGAGCAG
CAAACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AGGAAATGCG AGCACACCAG
GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCTAT CAAGAATAAA
GCAGAACGGG AAAGGCGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
GAAAGAAAGA GACTTGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
CTTGAACATC TAGAATTCCT AGAGAAACAG AATGAGCAGC TTTTGAAATC CTGTCATGCA
GTGTCCCAAA CGCAAGGCGA AGGAGATGCA GCAGATGGTG AAATTGGAAG CCGAGATGGA
CCGCAGACCA GCAACAGTAG TATGAAACTC CAAAATGCAA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14162
Clone ID Related Accession (Same CDS sequence) NM_182797
Accession Version NM_182797.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB270238.1, BC117458.1, DR761451.1 and AL117633.1. On or before Aug 31, 2013 this sequence version replaced gi:530425744, gi:33469938. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 3. The complete exon combination of the 5' UTR for this variant has not been determined. The resulting isoform (b) lacks an internal segment, compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC117458.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGGCCTGGTC
ACTGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTAGGTGC TACCACTAAT
ATCCATCCAT ATTTGTCCAC AATGATCAAC TACGCCCAGC CTGTAAAGTT TCAAGGTTTC
CATGTGGCAG AAGAACGCAA TATTCATTAT AACATGTCTT CTTTTAATGA ATCAGTCGGT
CTTGGCTACT TGAAGACACA TGCAATTGAA TTTGTCAATT ATAACAAACG GCAAATGAGT
CGCATTTACC CCAAGGGAGG CCGAGTCGAT TCCAGTAATT ACATGCCTCA GATTTTCTGG
AACGCTGGCT GCCAGATGGT TTCACTGAAC TATCAAACCC CAGATTTAGC GATGCAATTG
AATCAGGGAA AATTTGAGTA TAATGGATCG TGCGGGTACC TTCTCAAACC AGATTTCATG
AGGCGGCCTG ATCGAACATT TGACCCCTTC TCTGAAACTC CTGTTGATGG TGTTATTGCA
GCCACTTGCT CAGTGCAGGT TATATCAGGT CAATTCTTAT CAGATAAGAA AATTGGCACC
TACGTAGAGG TGGATATGTA TGGGTTGCCC ACTGACACCA TACGTAAGGA ATTCCGAACT
CGCATGGTTA TGAATAATGG ACTCAATCCA GTTTACAATG AAGAGTCATT TGTATTTCGG
AAGGTGATCC TGCCGGACCT GGCTGTCTTG AGAATAGCTG TGTATGATGA TAACAACAAG
CTGATTGGCC AGAGGATCCT CCCGCTTGAT GGCCTCCAAG CCGGATATCG ACACATTTCC
CTTCGAAATG AGGGAAATAA ACCATTATCA CTACCAACAA TTTTCTGCAA TATTGTTCTT
AAAACATATG TGCCTGATGG ATTTGGAGAT ATCGTGGATG CTTTATCAGA TCCAAAGAAA
TTTCTCTCAA TTACAGAAAA GAGAGCAGAC CAAATGAGAG CTATGGGCAT TGAAACTAGT
GACATAGCCG ACGTGCCCAG TGACACTTCC AAAAATGACA AGAAAGGAAA GGCCAACACC
GCCAAAGCAA ATGTGACCCC TCAGAGTAGC TCTGAGCTCA GACCAACCAC CACGGCTGCC
CTGGCCTCTG GTGTGGAAGC CAAGAAAGGT ATTGAACTTA TCCCTCAAGT AAGGATAGAA
GACTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAGCAGAA GGAGCTAAAT
TCTTTAAAGA AGAAACATGC AAAGGAACAC AGTACCATGC AGAAGTTACA CTGCACGCAA
GTTGACAAAA TTGTGGCACA GTATGACAAA GAGAAGTCGA CTCATGAGAA AATCCTAGAG
AAGGCAATGA AGAAGAAGGG GGGAAGTAAT TGTCTCGAAA TGAAAAAAGA AACAGAAATC
AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AAGAGATTGT AGCACAGCAC
ACAAAGGAAT GGTCAGAAAT GATCAATACC CACAGTGCTG AGGAGCAAGA AATCCGAGAC
CTGCACCTCA GCCAGCAGTG TGAGCTGCTG AAAAAGCTAC TCATCAATGC CCACGAGCAG
CAAACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AGGAAATGCG AGCACACCAG
GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCTAT CAAGAATAAA
GCAGAACGGG AAAGGCGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
GAAAGAAAGA GACTTGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
CTTGAACATC TAGAATTCCT AGAGAAACAG AATGAGCAGG CGAAGGAGAT GCAGCAGATG
GTGAAATTGG AAGCCGAGAT GGACCGCAGA CCAGCAACAG TAGTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_877949.2
CDS79..3606
Misc Feature(1)22..24(+)
Misc Feature(2)82..84(+)
Misc Feature(3)127..507(+)
Misc Feature(4)142..429(+)
Misc Feature(5)151..237(+)
Misc Feature(6)730..2499(+)
Misc Feature(7)739..1014(+)
Misc Feature(8)1012..2082(+)
Misc Feature(9)1060..1947(+)
Misc Feature(10)1060..1203(+)
Misc Feature(11)1063..1305(+)
Misc Feature(12)2182..2532(+)
Misc Feature(13)2233..2424(+)
Misc Feature(14)2734..2736(+)
Misc Feature(15)2803..2943(+)
Misc Feature(16)3220..3558(+)
Exon (1)1..63
Gene:PLCB4
Gene Synonym:
Exon (2)64..162
Gene:PLCB4
Gene Synonym:
Exon (3)163..243
Gene:PLCB4
Gene Synonym:
Exon (4)244..303
Gene:PLCB4
Gene Synonym:
Exon (5)304..447
Gene:PLCB4
Gene Synonym:
Exon (6)448..527
Gene:PLCB4
Gene Synonym:
Exon (7)528..581
Gene:PLCB4
Gene Synonym:
Exon (8)582..663
Gene:PLCB4
Gene Synonym:
Exon (9)664..764
Gene:PLCB4
Gene Synonym:
Exon (10)765..822
Gene:PLCB4
Gene Synonym:
Exon (11)823..931
Gene:PLCB4
Gene Synonym:
Exon (12)932..1142
Gene:PLCB4
Gene Synonym:
Exon (13)1143..1236
Gene:PLCB4
Gene Synonym:
Exon (14)1237..1316
Gene:PLCB4
Gene Synonym:
Exon (15)1317..1401
Gene:PLCB4
Gene Synonym:
Exon (16)1402..1492
Gene:PLCB4
Gene Synonym:
Exon (17)1493..1588
Gene:PLCB4
Gene Synonym:
Exon (18)1589..1689
Gene:PLCB4
Gene Synonym:
Exon (19)1690..1831
Gene:PLCB4
Gene Synonym:
Exon (20)1832..1916
Gene:PLCB4
Gene Synonym:
Exon (21)1917..2041
Gene:PLCB4
Gene Synonym:
Exon (22)2042..2093
Gene:PLCB4
Gene Synonym:
Exon (23)2094..2196
Gene:PLCB4
Gene Synonym:
Exon (24)2197..2361
Gene:PLCB4
Gene Synonym:
Exon (25)2362..2566
Gene:PLCB4
Gene Synonym:
Exon (26)2567..2655
Gene:PLCB4
Gene Synonym:
Exon (27)2656..2806
Gene:PLCB4
Gene Synonym:
Exon (28)2807..2856
Gene:PLCB4
Gene Synonym:
Exon (29)2857..2922
Gene:PLCB4
Gene Synonym:
Exon (30)2923..3038
Gene:PLCB4
Gene Synonym:
Exon (31)3039..3114
Gene:PLCB4
Gene Synonym:
Exon (32)3115..3290
Gene:PLCB4
Gene Synonym:
Exon (33)3291..3392
Gene:PLCB4
Gene Synonym:
Exon (34)3393..3450
Gene:PLCB4
Gene Synonym:
Exon (35)3451..3537
Gene:PLCB4
Gene Synonym:
Exon (36)3538..5433
Gene:PLCB4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_182797
Organism Homo sapiens (human)
Definition Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_182797

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGGCCTGGTC
ACTGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTAGGTGC TACCACTAAT
ATCCATCCAT ATTTGTCCAC AATGATCAAC TACGCCCAGC CTGTAAAGTT TCAAGGTTTC
CATGTGGCAG AAGAACGCAA TATTCATTAT AACATGTCTT CTTTTAATGA ATCAGTCGGT
CTTGGCTACT TGAAGACACA TGCAATTGAA TTTGTCAATT ATAACAAACG GCAAATGAGT
CGCATTTACC CCAAGGGAGG CCGAGTCGAT TCCAGTAATT ACATGCCTCA GATTTTCTGG
AACGCTGGCT GCCAGATGGT TTCACTGAAC TATCAAACCC CAGATTTAGC GATGCAATTG
AATCAGGGAA AATTTGAGTA TAATGGATCG TGCGGGTACC TTCTCAAACC AGATTTCATG
AGGCGGCCTG ATCGAACATT TGACCCCTTC TCTGAAACTC CTGTTGATGG TGTTATTGCA
GCCACTTGCT CAGTGCAGGT TATATCAGGT CAATTCTTAT CAGATAAGAA AATTGGCACC
TACGTAGAGG TGGATATGTA TGGGTTGCCC ACTGACACCA TACGTAAGGA ATTCCGAACT
CGCATGGTTA TGAATAATGG ACTCAATCCA GTTTACAATG AAGAGTCATT TGTATTTCGG
AAGGTGATCC TGCCGGACCT GGCTGTCTTG AGAATAGCTG TGTATGATGA TAACAACAAG
CTGATTGGCC AGAGGATCCT CCCGCTTGAT GGCCTCCAAG CCGGATATCG ACACATTTCC
CTTCGAAATG AGGGAAATAA ACCATTATCA CTACCAACAA TTTTCTGCAA TATTGTTCTT
AAAACATATG TGCCTGATGG ATTTGGAGAT ATCGTGGATG CTTTATCAGA TCCAAAGAAA
TTTCTCTCAA TTACAGAAAA GAGAGCAGAC CAAATGAGAG CTATGGGCAT TGAAACTAGT
GACATAGCCG ACGTGCCCAG TGACACTTCC AAAAATGACA AGAAAGGAAA GGCCAACACC
GCCAAAGCAA ATGTGACCCC TCAGAGTAGC TCTGAGCTCA GACCAACCAC CACGGCTGCC
CTGGCCTCTG GTGTGGAAGC CAAGAAAGGT ATTGAACTTA TCCCTCAAGT AAGGATAGAA
GACTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAGCAGAA GGAGCTAAAT
TCTTTAAAGA AGAAACATGC AAAGGAACAC AGTACCATGC AGAAGTTACA CTGCACGCAA
GTTGACAAAA TTGTGGCACA GTATGACAAA GAGAAGTCGA CTCATGAGAA AATCCTAGAG
AAGGCAATGA AGAAGAAGGG GGGAAGTAAT TGTCTCGAAA TGAAAAAAGA AACAGAAATC
AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AAGAGATTGT AGCACAGCAC
ACAAAGGAAT GGTCAGAAAT GATCAATACC CACAGTGCTG AGGAGCAAGA AATCCGAGAC
CTGCACCTCA GCCAGCAGTG TGAGCTGCTG AAAAAGCTAC TCATCAATGC CCACGAGCAG
CAAACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AGGAAATGCG AGCACACCAG
GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCTAT CAAGAATAAA
GCAGAACGGG AAAGGCGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
GAAAGAAAGA GACTTGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
CTTGAACATC TAGAATTCCT AGAGAAACAG AATGAGCAGG CGAAGGAGAT GCAGCAGATG
GTGAAATTGG AAGCCGAGAT GGACCGCAGA CCAGCAACAG TAGTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18159
Clone ID Related Accession (Same CDS sequence) NM_001172646
Accession Version NM_001172646.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform c
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK307837.1, DB270238.1, BC143868.1, DN913865.1 and AL117633.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (3) represents the longest transcript and encodes isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGGCGAA GGAGATGCAG CAGATGGTGA AATTGGAAGC CGAGATGGAC
CGCAGACCAG CAACAGTAGT ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001166117.1
CDS232..3795
Misc Feature(1)175..177(+)
Misc Feature(2)235..237(+)
Misc Feature(3)280..660(+)
Misc Feature(4)295..582(+)
Misc Feature(5)304..390(+)
Misc Feature(6)883..2688(+)
Misc Feature(7)892..1167(+)
Misc Feature(8)1165..2271(+)
Misc Feature(9)1213..2136(+)
Misc Feature(10)1213..1356(+)
Misc Feature(11)1216..1458(+)
Misc Feature(12)2371..2721(+)
Misc Feature(13)2422..2613(+)
Misc Feature(14)2923..2925(+)
Misc Feature(15)2992..3132(+)
Misc Feature(16)3409..3747(+)
Exon (1)1..153
Gene:PLCB4
Gene Synonym:
Exon (2)154..216
Gene:PLCB4
Gene Synonym:
Exon (3)217..315
Gene:PLCB4
Gene Synonym:
Exon (4)316..396
Gene:PLCB4
Gene Synonym:
Exon (5)397..456
Gene:PLCB4
Gene Synonym:
Exon (6)457..600
Gene:PLCB4
Gene Synonym:
Exon (7)601..680
Gene:PLCB4
Gene Synonym:
Exon (8)681..734
Gene:PLCB4
Gene Synonym:
Exon (9)735..816
Gene:PLCB4
Gene Synonym:
Exon (10)817..917
Gene:PLCB4
Gene Synonym:
Exon (11)918..975
Gene:PLCB4
Gene Synonym:
Exon (12)976..1084
Gene:PLCB4
Gene Synonym:
Exon (13)1085..1295
Gene:PLCB4
Gene Synonym:
Exon (14)1296..1389
Gene:PLCB4
Gene Synonym:
Exon (15)1390..1469
Gene:PLCB4
Gene Synonym:
Exon (16)1470..1554
Gene:PLCB4
Gene Synonym:
Exon (17)1555..1645
Gene:PLCB4
Gene Synonym:
Exon (18)1646..1741
Gene:PLCB4
Gene Synonym:
Exon (19)1742..1842
Gene:PLCB4
Gene Synonym:
Exon (20)1843..1878
Gene:PLCB4
Gene Synonym:
Exon (21)1879..2020
Gene:PLCB4
Gene Synonym:
Exon (22)2021..2105
Gene:PLCB4
Gene Synonym:
Exon (23)2106..2230
Gene:PLCB4
Gene Synonym:
Exon (24)2231..2282
Gene:PLCB4
Gene Synonym:
Exon (25)2283..2385
Gene:PLCB4
Gene Synonym:
Exon (26)2386..2550
Gene:PLCB4
Gene Synonym:
Exon (27)2551..2755
Gene:PLCB4
Gene Synonym:
Exon (28)2756..2844
Gene:PLCB4
Gene Synonym:
Exon (29)2845..2995
Gene:PLCB4
Gene Synonym:
Exon (30)2996..3045
Gene:PLCB4
Gene Synonym:
Exon (31)3046..3111
Gene:PLCB4
Gene Synonym:
Exon (32)3112..3227
Gene:PLCB4
Gene Synonym:
Exon (33)3228..3303
Gene:PLCB4
Gene Synonym:
Exon (34)3304..3479
Gene:PLCB4
Gene Synonym:
Exon (35)3480..3581
Gene:PLCB4
Gene Synonym:
Exon (36)3582..3639
Gene:PLCB4
Gene Synonym:
Exon (37)3640..3726
Gene:PLCB4
Gene Synonym:
Exon (38)3727..5622
Gene:PLCB4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001172646
Organism Homo sapiens (human)
Definition Homo sapiens phospholipase C, beta 4 (PLCB4), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001172646

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGGCGAA GGAGATGCAG CAGATGGTGA AATTGGAAGC CGAGATGGAC
CGCAGACCAG CAACAGTAGT ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
*
*
*
*