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PLN phospholamban [Homo sapiens (human)]

Gene Symbol PLN
Entrez Gene ID 5350
Full Name phospholamban
Synonyms CMD1P, CMH18, PLB
General protein information
Preferred Names
cardiac phospholamban
Names
cardiac phospholamban
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6q22.1

Summary The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

172405

Disorder Html: Cardiomyopathy, dilated, 1P, 609909 (3)





The following PLN gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLN gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu17925 NM_002667 Homo sapiens phospholamban (PLN), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu17925
Accession Version NM_002667.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 159bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu17925D_COA.pdf (pdf)
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cardiac phospholamban
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP259965.1, M63603.1 and BC005269.1. This sequence is a reference standard in the RefSeqGene project. On Jul 18, 2008 this sequence version replaced gi:29171725. Summary: The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005269.1, M63603.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
ATGGAGAAAG TCCAATACCT CACTCGCTCA GCTATAAGAA GAGCCTCAAC CATTGAAATG 
CCTCAACAAG CACGTCAAAA GCTACAGAAT CTATTTATCA ATTTCTGTCT CATCTTAATA
TGTCTCTTGC TGATCTGTAT CATCGTGATG CTTCTCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_002658.1
CDS212..370
Misc Feature(1)98..100(+)
Misc Feature(2)212..367(+)
Misc Feature(3)257..277(+)
Misc Feature(4)257..259(+)
Misc Feature(5)257..259(+)
Misc Feature(6)260..262(+)
Misc Feature(7)260..262(+)
Misc Feature(8)305..367(+)
Exon (1)1..114
Gene:PLN
Gene Synonym:
Exon (2)115..1714
Gene:PLN
Gene Synonym:
Translation
Position Chain Variation Link
59 59 c, t dbSNP:755267827
62 62 a, g dbSNP:9489434
70 70 a, t dbSNP:779232815
75 75 c, t dbSNP:748273819
92 92 c, t dbSNP:150080760
115 115 c, g dbSNP:558554251
126 126 c, g dbSNP:572092385
131 131 c, t dbSNP:541080130
149 149 c, t dbSNP:543790768
151 151 c, g dbSNP:186238926
152 152 c, t dbSNP:529837883
156 156 c, t dbSNP:189611501
161 161 a, t dbSNP:770154758
163 163 a, c, t dbSNP:773520639
168 168 c, t dbSNP:771080303
172 172 a, c, t dbSNP:774271388
173 173 a, c, g dbSNP:375430634
179 179 c, t dbSNP:372647899
184 184 a, t dbSNP:763754683
185 185 c, t dbSNP:753445677
199 199 c, t dbSNP:756839662
201 201 c, t dbSNP:764862475
208 208 c, t dbSNP:749900271
213 213 c, t dbSNP:727503374
215 215 c, g dbSNP:758364608
222 222 -, c dbSNP:774186147
227 227 c, g, t dbSNP:780082801
236 236 c, t dbSNP:111033559
237 237 a, g dbSNP:754782171
238 238 c, t dbSNP:145623013
245 245 a, g dbSNP:749571694
246 246 c, t dbSNP:771044756
247 247 -, aag dbSNP:778096369
247 247 a, g dbSNP:774556120
248 248 -, a dbSNP:727503375
251 251 -, aga dbSNP:397516784
252 252 g, t dbSNP:151112761
254 254 a, g dbSNP:397516785
272 272 a, c dbSNP:397516786
280 280 a, g dbSNP:775923421
284 284 c, t dbSNP:761056344
311 311 a, c dbSNP:745592199
315 315 -, tc dbSNP:747947483
327 327 g, t dbSNP:111033560
328 328 a, g dbSNP:776969887
352 352 c, g dbSNP:552078051
355 355 c, t dbSNP:141114252
356 356 a, g dbSNP:749962743
359 359 a, c dbSNP:757797900
362 362 c, t dbSNP:766452369
363 363 c, t dbSNP:397516783
374 374 a, t dbSNP:397516782
384 384 c, t dbSNP:371507549
390 390 g, t dbSNP:754942529
392 392 c, t dbSNP:781189307
397 397 a, c dbSNP:747890379
398 398 c, g dbSNP:372970382
417 417 a, g dbSNP:778941150
420 420 c, t dbSNP:745978438
421 421 g, t dbSNP:369368451
429 429 a, g dbSNP:544803483
450 450 c, t dbSNP:565692601
459 459 a, g dbSNP:28763979
505 505 c, t dbSNP:780385491
543 543 c, t dbSNP:180799194
583 583 c, t dbSNP:747130253
594 594 a, c dbSNP:35739264
598 598 a, g dbSNP:568262125
599 599 c, t dbSNP:532539997
620 620 c, t dbSNP:79199092
647 647 a, g dbSNP:768837853
673 673 a, g dbSNP:557568289
679 679 a, g dbSNP:570972632
700 700 c, g dbSNP:186663539
753 753 c, t dbSNP:553705193
767 767 a, g, t dbSNP:12198461
785 785 c, t dbSNP:117280667
804 804 -, cac dbSNP:765386927
837 837 a, g dbSNP:760027606
938 938 c, t dbSNP:554698802
939 939 a, t dbSNP:191057969
1059 1059 c, t dbSNP:543235354
1123 1123 a, g dbSNP:748689128
1153 1153 c, t dbSNP:563461651
1165 1165 a, t dbSNP:75600410
1182 1182 c, t dbSNP:537697221
1209 1209 -, tc dbSNP:141447190
1231 1231 g, t dbSNP:371473315
1236 1236 c, t dbSNP:147557787
1279 1279 c, t dbSNP:142301036
1347 1347 a, g dbSNP:113584707
1360 1360 a, t dbSNP:557687689
1368 1368 a, c dbSNP:574434391
1377 1377 a, g, t dbSNP:528466147
1378 1378 a, t dbSNP:548206730
1382 1382 a, t dbSNP:183097878
1395 1395 c, g dbSNP:760850270
1440 1440 a, t dbSNP:562026339
1447 1447 a, c dbSNP:527452464
1468 1468 c, t dbSNP:185945888
1481 1481 c, t dbSNP:550818264
1484 1484 g, t dbSNP:570977086
1506 1506 a, g dbSNP:764978489
1513 1513 c, t dbSNP:775287766
1514 1514 a, g dbSNP:549223893
1532 1532 a, t dbSNP:770271766
1536 1536 c, g dbSNP:762819979
1550 1550 a, g dbSNP:540046916
1613 1613 c, t dbSNP:146145000
1649 1649 a, g dbSNP:537800022
1653 1653 a, t dbSNP:201177617
1655 1655 a, t dbSNP:201951020
1656 1656 a, t dbSNP:200551023
1658 1658 a, t dbSNP:201396273
1677 1677 a, t dbSNP:763768705
1707 1707 a, g dbSNP:41291942
1714 1714 a, g dbSNP:571117883

Target ORF information:

RefSeq Version NM_002667
Organism Homo sapiens (human)
Definition Homo sapiens phospholamban (PLN), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002667

ORF Insert Sequence:

1
61
121
ATGGAGAAAG TCCAATACCT CACTCGCTCA GCTATAAGAA GAGCCTCAAC CATTGAAATG 
CCTCAACAAG CACGTCAAAA GCTACAGAAT CTATTTATCA ATTTCTGTCT CATCTTAATA
TGTCTCTTGC TGATCTGTAT CATCGTGATG CTTCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.