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PLOD1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol PLOD1
Entrez Gene ID 5351
Full Name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1
Synonyms EDS6, LH, LH1, LLH, PLOD
General protein information
Preferred Names
procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
Names
procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
lysine hydroxylase
lysyl hydroxlase 1
procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1
NP_000293.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p36.22

Summary Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

153454

Disorder Html: Ehlers-Danlos syndrome, type VI, 225400 (3); Nevo syndrome, 601451

mRNA and Protein(s)

mRNA Protein Name
XM_011541593 XP_011539895 procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 isoform X1
XM_011541594 XP_011539896 procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 isoform X2
NM_000302 NP_000293 procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 precursor



Homo sapiens (human) PLOD1 NP_000293.2
Pan troglodytes (chimpanzee) PLOD1 XP_001142788.1
Canis lupus familiaris (dog) PLOD1 XP_544565.2
Bos taurus (cattle) PLOD1 NP_776573.1
Mus musculus (house mouse) Plod1 NP_035252.1
Rattus norvegicus (Norway rat) Plod1 NP_446279.2
Gallus gallus (chicken) PLOD1 NP_001005618.1
Danio rerio (zebrafish) plod1a NP_001071210.2
Xenopus (Silurana) tropicalis (western clawed frog) plod1 NP_001072340.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PLOD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLOD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu59781 XM_011541593 PREDICTED: Homo sapiens procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu59782 XM_011541594 PREDICTED: Homo sapiens procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu18408
NM_000302 Homo sapiens procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (PLOD1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu59781
Clone ID Related Accession (Same CDS sequence) XM_011541593
Accession Version XM_011541593.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2325bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_032977.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGCGGCCCC TGCTGCTACT GGCCCTGCTG GGCTGGCTGC TGCTGGCCGA AGCGAAGGGC 
GACGCCAAGC CGGAGGCCCC CTGTTGCCAA GAGGGCCTCA GAGCTGGTGG CTCTGGTTCT
CTTCACCTGG GGAGAGACTT CACCGTCCTG GCAGGAGCTC GGGGGAGCCC TTCCCCAAGT
GTGAGCAGCA TCCCTCGTTT CTGGATCCCA GGTTCAGACA ACCTTTTAGT CCTCACGGTG
GCCACTAAGG AGACCGAGGG ATTCCGTCGC TTCAAGCGCT CAGCTCAGTT CTTCAACTAC
AAGATCCAGG CGCTTGGCCT AGGGGAGGAC TGGAATGTGG AGAAGGGGAC GTCGGCAGGT
GGAGGGCAGA AGGTCCGGCT GCTGAAGAAA GCTCTGGAGA AGCACGCAGA CAAGGAGGAT
CTGGTCATTC TCTTCGCAGA CAGCTATGAC GTGCTGTTTG CATCGGGGCC CCGGGAGCTC
CTGAAGAAGT TCCGGCAGGC CAGGAGCCAG GTGGTCTTCT CTGCTGAGGA GCTCATCTAC
CCAGACCGCA GGCTGGAGAC CAAGTATCCG GTGGTGTCCG ATGGCAAGAG GTTCCTGGGC
TCTGGAGGCT TCATCGGTTA TGCCCCCAAC CTCAGCAAAC TGGTGGCCGA GTGGGAGGGC
CAGGACAGCG ACAGCGATCA GCTGTTTTAC ACCAAGATCT TCTTGGACCC GGAGAAGAGG
GAGCAGATCA ATATCACCCT GGACCACCGC TGCCGTATCT TCCAGAACCT GGATGGAGCC
TTGGATGAGG TCGTGCTCAA GTTTGAAATG GGCCATGTGA GAGCGAGGAA CCTGGCCTAT
GACACCCTCC CGGTCCTGAT CCATGGCAAC GGGCCAACCA AGCTGCAGTT GAACTACCTG
GGCAACTACA TCCCGCGCTT CTGGACCTTC GAAACAGGCT GCACCGTGTG TGACGAAGGC
TTGCGCAGCC TCAAGGGCAT TGGGGATGAA GCTCTGCCCA CGGTCCTGGT CGGCGTGTTC
ATCGAACAGC CCACGCCGTT TGTGTCCCTG TTCTTCCAGC GGCTCCTGCG GCTCCACTAC
CCCCAGAAAC ACATGCGACT TTTCATCCAC AACCACGAGC AGCACCACAA GGCTCAGGTG
GAAGAGTTCC TGGCACAGCA TGGCAGCGAG TACCAGTCTG TGAAGCTGGT GGGCCCTGAG
GTGCGGATGG CGAATGCAGA TGCCAGGAAC ATGGGCGCAG ACCTGTGCCG GCAGGACCGC
AGCTGCACCT ACTACTTCAG CGTGGATGCT GACGTGGCCC TGACCGAGCC CAACAGCCTG
CGGCTGCTGA TCCAACAGAA CAAGAACGTC ATTGCCCCGC TGATGACCCG GCATGGGAGG
CTGTGGTCGA ACTTCTGGGG GGCTCTCAGT GCAGATGGCT ACTATGCCCG TTCCGAGGAC
TACGTGGACA TTGTGCAGGG GCGGCGTGTT GGTGTCTGGA ATGTGCCCTA TATTTCAAAC
ATCTACTTGA TCAAGGGCAG TGCCCTGCGG GGTGAGCTGC AGTCCTCAGA TCTCTTCCAC
CACAGCAAGC TGGACCCCGA CATGGCCTTC TGTGCCAACA TCCGGCAGCA GGATGTGTTC
ATGTTCCTGA CCAACCGGCA CACCCTTGGC CATCTGCTCT CCCTAGACAG CTACCGCACC
ACCCACCTGC ACAACGACCT CTGGGAGGTG TTCAGCAACC CCGAGGACTG GAAGGAGAAG
TACATCCACC AGAACTACAC CAAAGCCCTG GCAGGGAAGC TGGTGGAGAC GCCCTGCCCG
GATGTCTATT GGTTCCCCAT CTTCACGGAG GTGGCCTGTG ATGAGCTGGT GGAGGAGATG
GAGCACTTTG GCCAGTGGTC TCTGGGCAAC AACAAGGACA ACCGCATCCA GGGTGGCTAC
GAGAACGTGC CGACTATTGA CATCCACATG AACCAGATCG GCTTTGAGCG GGAGTGGCAC
AAATTCCTGC TGGAGTACAT TGCGCCCATG ACGGAGAAGC TCTACCCCGG CTACTACACC
AGGGCCCAGT TTGACCTGGC CTTTGTCGTC CGCTACAAGC CTGATGAGCA GCCCTCACTG
ATGCCACACC ATGATGCCTC CACCTTCACC ATCAACATCG CCCTGAACCG AGTCGGGGTG
GATTACGAGG GCGGGGGCTG TCGGTTCCTG CGCTACAACT GTTCCATCCG AGCCCCAAGG
AAGGGCTGGA CCCTCATGCA CCCTGGACGA CTCACGCATT ACCATGAGGG GCTCCCCACC
ACCAGGGGCA CCCGCTACAT CGCAGTCTCC TTCGTCGATC CCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011539895.1
CDS50..2374
Misc Feature(1)1874..2365(+)
Translation

Target ORF information:

RefSeq Version XM_011541593
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011541593

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGCGGCCCC TGCTGCTACT GGCCCTGCTG GGCTGGCTGC TGCTGGCCGA AGCGAAGGGC 
GACGCCAAGC CGGAGGCCCC CTGTTGCCAA GAGGGCCTCA GAGCTGGTGG CTCTGGTTCT
CTTCACCTGG GGAGAGACTT CACCGTCCTG GCAGGAGCTC GGGGGAGCCC TTCCCCAAGT
GTGAGCAGCA TCCCTCGTTT CTGGATCCCA GGTTCAGACA ACCTTTTAGT CCTCACGGTG
GCCACTAAGG AGACCGAGGG ATTCCGTCGC TTCAAGCGCT CAGCTCAGTT CTTCAACTAC
AAGATCCAGG CGCTTGGCCT AGGGGAGGAC TGGAATGTGG AGAAGGGGAC GTCGGCAGGT
GGAGGGCAGA AGGTCCGGCT GCTGAAGAAA GCTCTGGAGA AGCACGCAGA CAAGGAGGAT
CTGGTCATTC TCTTCGCAGA CAGCTATGAC GTGCTGTTTG CATCGGGGCC CCGGGAGCTC
CTGAAGAAGT TCCGGCAGGC CAGGAGCCAG GTGGTCTTCT CTGCTGAGGA GCTCATCTAC
CCAGACCGCA GGCTGGAGAC CAAGTATCCG GTGGTGTCCG ATGGCAAGAG GTTCCTGGGC
TCTGGAGGCT TCATCGGTTA TGCCCCCAAC CTCAGCAAAC TGGTGGCCGA GTGGGAGGGC
CAGGACAGCG ACAGCGATCA GCTGTTTTAC ACCAAGATCT TCTTGGACCC GGAGAAGAGG
GAGCAGATCA ATATCACCCT GGACCACCGC TGCCGTATCT TCCAGAACCT GGATGGAGCC
TTGGATGAGG TCGTGCTCAA GTTTGAAATG GGCCATGTGA GAGCGAGGAA CCTGGCCTAT
GACACCCTCC CGGTCCTGAT CCATGGCAAC GGGCCAACCA AGCTGCAGTT GAACTACCTG
GGCAACTACA TCCCGCGCTT CTGGACCTTC GAAACAGGCT GCACCGTGTG TGACGAAGGC
TTGCGCAGCC TCAAGGGCAT TGGGGATGAA GCTCTGCCCA CGGTCCTGGT CGGCGTGTTC
ATCGAACAGC CCACGCCGTT TGTGTCCCTG TTCTTCCAGC GGCTCCTGCG GCTCCACTAC
CCCCAGAAAC ACATGCGACT TTTCATCCAC AACCACGAGC AGCACCACAA GGCTCAGGTG
GAAGAGTTCC TGGCACAGCA TGGCAGCGAG TACCAGTCTG TGAAGCTGGT GGGCCCTGAG
GTGCGGATGG CGAATGCAGA TGCCAGGAAC ATGGGCGCAG ACCTGTGCCG GCAGGACCGC
AGCTGCACCT ACTACTTCAG CGTGGATGCT GACGTGGCCC TGACCGAGCC CAACAGCCTG
CGGCTGCTGA TCCAACAGAA CAAGAACGTC ATTGCCCCGC TGATGACCCG GCATGGGAGG
CTGTGGTCGA ACTTCTGGGG GGCTCTCAGT GCAGATGGCT ACTATGCCCG TTCCGAGGAC
TACGTGGACA TTGTGCAGGG GCGGCGTGTT GGTGTCTGGA ATGTGCCCTA TATTTCAAAC
ATCTACTTGA TCAAGGGCAG TGCCCTGCGG GGTGAGCTGC AGTCCTCAGA TCTCTTCCAC
CACAGCAAGC TGGACCCCGA CATGGCCTTC TGTGCCAACA TCCGGCAGCA GGATGTGTTC
ATGTTCCTGA CCAACCGGCA CACCCTTGGC CATCTGCTCT CCCTAGACAG CTACCGCACC
ACCCACCTGC ACAACGACCT CTGGGAGGTG TTCAGCAACC CCGAGGACTG GAAGGAGAAG
TACATCCACC AGAACTACAC CAAAGCCCTG GCAGGGAAGC TGGTGGAGAC GCCCTGCCCG
GATGTCTATT GGTTCCCCAT CTTCACGGAG GTGGCCTGTG ATGAGCTGGT GGAGGAGATG
GAGCACTTTG GCCAGTGGTC TCTGGGCAAC AACAAGGACA ACCGCATCCA GGGTGGCTAC
GAGAACGTGC CGACTATTGA CATCCACATG AACCAGATCG GCTTTGAGCG GGAGTGGCAC
AAATTCCTGC TGGAGTACAT TGCGCCCATG ACGGAGAAGC TCTACCCCGG CTACTACACC
AGGGCCCAGT TTGACCTGGC CTTTGTCGTC CGCTACAAGC CTGATGAGCA GCCCTCACTG
ATGCCACACC ATGATGCCTC CACCTTCACC ATCAACATCG CCCTGAACCG AGTCGGGGTG
GATTACGAGG GCGGGGGCTG TCGGTTCCTG CGCTACAACT GTTCCATCCG AGCCCCAAGG
AAGGGCTGGA CCCTCATGCA CCCTGGACGA CTCACGCATT ACCATGAGGG GCTCCCCACC
ACCAGGGGCA CCCGCTACAT CGCAGTCTCC TTCGTCGATC CCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59782
Clone ID Related Accession (Same CDS sequence) XM_011541594
Accession Version XM_011541594.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2265bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_032977.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGATGAAGC CCGCAGCCCC CTGTTGCCAA GAGGGCCTCA GAGCTGGTGG CTCTGGTTCT 
CTTCACCTGG GGAGAGACTT CACCGTCCTG GCAGGAGCTC GGGGGAGCCC TTCCCCAAGT
GTGAGCAGCA TCCCTCGTTT CTGGATCCCA GGTTCAGACA ACCTTTTAGT CCTCACGGTG
GCCACTAAGG AGACCGAGGG ATTCCGTCGC TTCAAGCGCT CAGCTCAGTT CTTCAACTAC
AAGATCCAGG CGCTTGGCCT AGGGGAGGAC TGGAATGTGG AGAAGGGGAC GTCGGCAGGT
GGAGGGCAGA AGGTCCGGCT GCTGAAGAAA GCTCTGGAGA AGCACGCAGA CAAGGAGGAT
CTGGTCATTC TCTTCGCAGA CAGCTATGAC GTGCTGTTTG CATCGGGGCC CCGGGAGCTC
CTGAAGAAGT TCCGGCAGGC CAGGAGCCAG GTGGTCTTCT CTGCTGAGGA GCTCATCTAC
CCAGACCGCA GGCTGGAGAC CAAGTATCCG GTGGTGTCCG ATGGCAAGAG GTTCCTGGGC
TCTGGAGGCT TCATCGGTTA TGCCCCCAAC CTCAGCAAAC TGGTGGCCGA GTGGGAGGGC
CAGGACAGCG ACAGCGATCA GCTGTTTTAC ACCAAGATCT TCTTGGACCC GGAGAAGAGG
GAGCAGATCA ATATCACCCT GGACCACCGC TGCCGTATCT TCCAGAACCT GGATGGAGCC
TTGGATGAGG TCGTGCTCAA GTTTGAAATG GGCCATGTGA GAGCGAGGAA CCTGGCCTAT
GACACCCTCC CGGTCCTGAT CCATGGCAAC GGGCCAACCA AGCTGCAGTT GAACTACCTG
GGCAACTACA TCCCGCGCTT CTGGACCTTC GAAACAGGCT GCACCGTGTG TGACGAAGGC
TTGCGCAGCC TCAAGGGCAT TGGGGATGAA GCTCTGCCCA CGGTCCTGGT CGGCGTGTTC
ATCGAACAGC CCACGCCGTT TGTGTCCCTG TTCTTCCAGC GGCTCCTGCG GCTCCACTAC
CCCCAGAAAC ACATGCGACT TTTCATCCAC AACCACGAGC AGCACCACAA GGCTCAGGTG
GAAGAGTTCC TGGCACAGCA TGGCAGCGAG TACCAGTCTG TGAAGCTGGT GGGCCCTGAG
GTGCGGATGG CGAATGCAGA TGCCAGGAAC ATGGGCGCAG ACCTGTGCCG GCAGGACCGC
AGCTGCACCT ACTACTTCAG CGTGGATGCT GACGTGGCCC TGACCGAGCC CAACAGCCTG
CGGCTGCTGA TCCAACAGAA CAAGAACGTC ATTGCCCCGC TGATGACCCG GCATGGGAGG
CTGTGGTCGA ACTTCTGGGG GGCTCTCAGT GCAGATGGCT ACTATGCCCG TTCCGAGGAC
TACGTGGACA TTGTGCAGGG GCGGCGTGTT GGTGTCTGGA ATGTGCCCTA TATTTCAAAC
ATCTACTTGA TCAAGGGCAG TGCCCTGCGG GGTGAGCTGC AGTCCTCAGA TCTCTTCCAC
CACAGCAAGC TGGACCCCGA CATGGCCTTC TGTGCCAACA TCCGGCAGCA GGATGTGTTC
ATGTTCCTGA CCAACCGGCA CACCCTTGGC CATCTGCTCT CCCTAGACAG CTACCGCACC
ACCCACCTGC ACAACGACCT CTGGGAGGTG TTCAGCAACC CCGAGGACTG GAAGGAGAAG
TACATCCACC AGAACTACAC CAAAGCCCTG GCAGGGAAGC TGGTGGAGAC GCCCTGCCCG
GATGTCTATT GGTTCCCCAT CTTCACGGAG GTGGCCTGTG ATGAGCTGGT GGAGGAGATG
GAGCACTTTG GCCAGTGGTC TCTGGGCAAC AACAAGGACA ACCGCATCCA GGGTGGCTAC
GAGAACGTGC CGACTATTGA CATCCACATG AACCAGATCG GCTTTGAGCG GGAGTGGCAC
AAATTCCTGC TGGAGTACAT TGCGCCCATG ACGGAGAAGC TCTACCCCGG CTACTACACC
AGGGCCCAGT TTGACCTGGC CTTTGTCGTC CGCTACAAGC CTGATGAGCA GCCCTCACTG
ATGCCACACC ATGATGCCTC CACCTTCACC ATCAACATCG CCCTGAACCG AGTCGGGGTG
GATTACGAGG GCGGGGGCTG TCGGTTCCTG CGCTACAACT GTTCCATCCG AGCCCCAAGG
AAGGGCTGGA CCCTCATGCA CCCTGGACGA CTCACGCATT ACCATGAGGG GCTCCCCACC
ACCAGGGGCA CCCGCTACAT CGCAGTCTCC TTCGTCGATC CCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011539896.1
CDS13..2277
Misc Feature(1)1777..2268(+)
Translation

Target ORF information:

RefSeq Version XM_011541594
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011541594

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGATGAAGC CCGCAGCCCC CTGTTGCCAA GAGGGCCTCA GAGCTGGTGG CTCTGGTTCT 
CTTCACCTGG GGAGAGACTT CACCGTCCTG GCAGGAGCTC GGGGGAGCCC TTCCCCAAGT
GTGAGCAGCA TCCCTCGTTT CTGGATCCCA GGTTCAGACA ACCTTTTAGT CCTCACGGTG
GCCACTAAGG AGACCGAGGG ATTCCGTCGC TTCAAGCGCT CAGCTCAGTT CTTCAACTAC
AAGATCCAGG CGCTTGGCCT AGGGGAGGAC TGGAATGTGG AGAAGGGGAC GTCGGCAGGT
GGAGGGCAGA AGGTCCGGCT GCTGAAGAAA GCTCTGGAGA AGCACGCAGA CAAGGAGGAT
CTGGTCATTC TCTTCGCAGA CAGCTATGAC GTGCTGTTTG CATCGGGGCC CCGGGAGCTC
CTGAAGAAGT TCCGGCAGGC CAGGAGCCAG GTGGTCTTCT CTGCTGAGGA GCTCATCTAC
CCAGACCGCA GGCTGGAGAC CAAGTATCCG GTGGTGTCCG ATGGCAAGAG GTTCCTGGGC
TCTGGAGGCT TCATCGGTTA TGCCCCCAAC CTCAGCAAAC TGGTGGCCGA GTGGGAGGGC
CAGGACAGCG ACAGCGATCA GCTGTTTTAC ACCAAGATCT TCTTGGACCC GGAGAAGAGG
GAGCAGATCA ATATCACCCT GGACCACCGC TGCCGTATCT TCCAGAACCT GGATGGAGCC
TTGGATGAGG TCGTGCTCAA GTTTGAAATG GGCCATGTGA GAGCGAGGAA CCTGGCCTAT
GACACCCTCC CGGTCCTGAT CCATGGCAAC GGGCCAACCA AGCTGCAGTT GAACTACCTG
GGCAACTACA TCCCGCGCTT CTGGACCTTC GAAACAGGCT GCACCGTGTG TGACGAAGGC
TTGCGCAGCC TCAAGGGCAT TGGGGATGAA GCTCTGCCCA CGGTCCTGGT CGGCGTGTTC
ATCGAACAGC CCACGCCGTT TGTGTCCCTG TTCTTCCAGC GGCTCCTGCG GCTCCACTAC
CCCCAGAAAC ACATGCGACT TTTCATCCAC AACCACGAGC AGCACCACAA GGCTCAGGTG
GAAGAGTTCC TGGCACAGCA TGGCAGCGAG TACCAGTCTG TGAAGCTGGT GGGCCCTGAG
GTGCGGATGG CGAATGCAGA TGCCAGGAAC ATGGGCGCAG ACCTGTGCCG GCAGGACCGC
AGCTGCACCT ACTACTTCAG CGTGGATGCT GACGTGGCCC TGACCGAGCC CAACAGCCTG
CGGCTGCTGA TCCAACAGAA CAAGAACGTC ATTGCCCCGC TGATGACCCG GCATGGGAGG
CTGTGGTCGA ACTTCTGGGG GGCTCTCAGT GCAGATGGCT ACTATGCCCG TTCCGAGGAC
TACGTGGACA TTGTGCAGGG GCGGCGTGTT GGTGTCTGGA ATGTGCCCTA TATTTCAAAC
ATCTACTTGA TCAAGGGCAG TGCCCTGCGG GGTGAGCTGC AGTCCTCAGA TCTCTTCCAC
CACAGCAAGC TGGACCCCGA CATGGCCTTC TGTGCCAACA TCCGGCAGCA GGATGTGTTC
ATGTTCCTGA CCAACCGGCA CACCCTTGGC CATCTGCTCT CCCTAGACAG CTACCGCACC
ACCCACCTGC ACAACGACCT CTGGGAGGTG TTCAGCAACC CCGAGGACTG GAAGGAGAAG
TACATCCACC AGAACTACAC CAAAGCCCTG GCAGGGAAGC TGGTGGAGAC GCCCTGCCCG
GATGTCTATT GGTTCCCCAT CTTCACGGAG GTGGCCTGTG ATGAGCTGGT GGAGGAGATG
GAGCACTTTG GCCAGTGGTC TCTGGGCAAC AACAAGGACA ACCGCATCCA GGGTGGCTAC
GAGAACGTGC CGACTATTGA CATCCACATG AACCAGATCG GCTTTGAGCG GGAGTGGCAC
AAATTCCTGC TGGAGTACAT TGCGCCCATG ACGGAGAAGC TCTACCCCGG CTACTACACC
AGGGCCCAGT TTGACCTGGC CTTTGTCGTC CGCTACAAGC CTGATGAGCA GCCCTCACTG
ATGCCACACC ATGATGCCTC CACCTTCACC ATCAACATCG CCCTGAACCG AGTCGGGGTG
GATTACGAGG GCGGGGGCTG TCGGTTCCTG CGCTACAACT GTTCCATCCG AGCCCCAAGG
AAGGGCTGGA CCCTCATGCA CCCTGGACGA CTCACGCATT ACCATGAGGG GCTCCCCACC
ACCAGGGGCA CCCGCTACAT CGCAGTCTCC TTCGTCGATC CCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18408
Clone ID Related Accession (Same CDS sequence) NM_000302
Accession Version NM_000302.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2184bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC334246.1, M98252.1 and BQ447373.1. This sequence is a reference standard in the RefSeqGene project. On Mar 1, 2011 this sequence version replaced gi:32307143. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M98252.1, BC016657.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGCGGCCCC TGCTGCTACT GGCCCTGCTG GGCTGGCTGC TGCTGGCCGA AGCGAAGGGC 
GACGCCAAGC CGGAGGACAA CCTTTTAGTC CTCACGGTGG CCACTAAGGA GACCGAGGGA
TTCCGTCGCT TCAAGCGCTC AGCTCAGTTC TTCAACTACA AGATCCAGGC GCTTGGCCTA
GGGGAGGACT GGAATGTGGA GAAGGGGACG TCGGCAGGTG GAGGGCAGAA GGTCCGGCTG
CTGAAGAAAG CTCTGGAGAA GCACGCAGAC AAGGAGGATC TGGTCATTCT CTTCGCAGAC
AGCTATGACG TGCTGTTTGC ATCGGGGCCC CGGGAGCTCC TGAAGAAGTT CCGGCAGGCC
AGGAGCCAGG TGGTCTTCTC TGCTGAGGAG CTCATCTACC CAGACCGCAG GCTGGAGACC
AAGTATCCGG TGGTGTCCGA TGGCAAGAGG TTCCTGGGCT CTGGAGGCTT CATCGGTTAT
GCCCCCAACC TCAGCAAACT GGTGGCCGAG TGGGAGGGCC AGGACAGCGA CAGCGATCAG
CTGTTTTACA CCAAGATCTT CTTGGACCCG GAGAAGAGGG AGCAGATCAA TATCACCCTG
GACCACCGCT GCCGTATCTT CCAGAACCTG GATGGAGCCT TGGATGAGGT CGTGCTCAAG
TTTGAAATGG GCCATGTGAG AGCGAGGAAC CTGGCCTATG ACACCCTCCC GGTCCTGATC
CATGGCAACG GGCCAACCAA GCTGCAGTTG AACTACCTGG GCAACTACAT CCCGCGCTTC
TGGACCTTCG AAACAGGCTG CACCGTGTGT GACGAAGGCT TGCGCAGCCT CAAGGGCATT
GGGGATGAAG CTCTGCCCAC GGTCCTGGTC GGCGTGTTCA TCGAACAGCC CACGCCGTTT
GTGTCCCTGT TCTTCCAGCG GCTCCTGCGG CTCCACTACC CCCAGAAACA CATGCGACTT
TTCATCCACA ACCACGAGCA GCACCACAAG GCTCAGGTGG AAGAGTTCCT GGCACAGCAT
GGCAGCGAGT ACCAGTCTGT GAAGCTGGTG GGCCCTGAGG TGCGGATGGC GAATGCAGAT
GCCAGGAACA TGGGCGCAGA CCTGTGCCGG CAGGACCGCA GCTGCACCTA CTACTTCAGC
GTGGATGCTG ACGTGGCCCT GACCGAGCCC AACAGCCTGC GGCTGCTGAT CCAACAGAAC
AAGAACGTCA TTGCCCCGCT GATGACCCGG CATGGGAGGC TGTGGTCGAA CTTCTGGGGG
GCTCTCAGTG CAGATGGCTA CTATGCCCGT TCCGAGGACT ACGTGGACAT TGTGCAGGGG
CGGCGTGTTG GTGTCTGGAA TGTGCCCTAT ATTTCAAACA TCTACTTGAT CAAGGGCAGT
GCCCTGCGGG GTGAGCTGCA GTCCTCAGAT CTCTTCCACC ACAGCAAGCT GGACCCCGAC
ATGGCCTTCT GTGCCAACAT CCGGCAGCAG GATGTGTTCA TGTTCCTGAC CAACCGGCAC
ACCCTTGGCC ATCTGCTCTC CCTAGACAGC TACCGCACCA CCCACCTGCA CAACGACCTC
TGGGAGGTGT TCAGCAACCC CGAGGACTGG AAGGAGAAGT ACATCCACCA GAACTACACC
AAAGCCCTGG CAGGGAAGCT GGTGGAGACG CCCTGCCCGG ATGTCTATTG GTTCCCCATC
TTCACGGAGG TGGCCTGTGA TGAGCTGGTG GAGGAGATGG AGCACTTTGG CCAGTGGTCT
CTGGGCAACA ACAAGGACAA CCGCATCCAG GGTGGCTACG AGAACGTGCC GACTATTGAC
ATCCACATGA ACCAGATCGG CTTTGAGCGG GAGTGGCACA AATTCCTGCT GGAGTACATT
GCGCCCATGA CGGAGAAGCT CTACCCCGGC TACTACACCA GGGCCCAGTT TGACCTGGCC
TTTGTCGTCC GCTACAAGCC TGATGAGCAG CCCTCACTGA TGCCACACCA TGATGCCTCC
ACCTTCACCA TCAACATCGC CCTGAACCGA GTCGGGGTGG ATTACGAGGG CGGGGGCTGT
CGGTTCCTGC GCTACAACTG TTCCATCCGA GCCCCAAGGA AGGGCTGGAC CCTCATGCAC
CCTGGACGAC TCACGCATTA CCATGAGGGG CTCCCCACCA CCAGGGGCAC CCGCTACATC
GCAGTCTCCT TCGTCGATCC CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000293.2
CDS114..2297
Misc Feature(1)1797..2288(+)
Exon (1)1..189
Gene:PLOD1
Gene Synonym:
Exon (2)190..281
Gene:PLOD1
Gene Synonym:
Exon (3)282..415
Gene:PLOD1
Gene Synonym:
Exon (4)416..579
Gene:PLOD1
Gene Synonym:
Exon (5)580..692
Gene:PLOD1
Gene Synonym:
Exon (6)693..756
Gene:PLOD1
Gene Synonym:
Exon (7)757..854
Gene:PLOD1
Gene Synonym:
Exon (8)855..956
Gene:PLOD1
Gene Synonym:
Exon (9)957..1088
Gene:PLOD1
Gene Synonym:
Exon (10)1089..1210
Gene:PLOD1
Gene Synonym:
Exon (11)1211..1315
Gene:PLOD1
Gene Synonym:
Exon (12)1316..1441
Gene:PLOD1
Gene Synonym:
Exon (13)1442..1583
Gene:PLOD1
Gene Synonym:
Exon (14)1584..1697
Gene:PLOD1
Gene Synonym:
Exon (15)1698..1763
Gene:PLOD1
Gene Synonym:
Exon (16)1764..1868
Gene:PLOD1
Gene Synonym:
Exon (17)1869..2015
Gene:PLOD1
Gene Synonym:
Exon (18)2016..2141
Gene:PLOD1
Gene Synonym:
Exon (19)2142..3031
Gene:PLOD1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000302
Organism Homo sapiens (human)
Definition Homo sapiens procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (PLOD1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000302

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGCGGCCCC TGCTGCTACT GGCCCTGCTG GGCTGGCTGC TGCTGGCCGA AGCGAAGGGC 
GACGCCAAGC CGGAGGACAA CCTTTTAGTC CTCACGGTGG CCACTAAGGA GACCGAGGGA
TTCCGTCGCT TCAAGCGCTC AGCTCAGTTC TTCAACTACA AGATCCAGGC GCTTGGCCTA
GGGGAGGACT GGAATGTGGA GAAGGGGACG TCGGCAGGTG GAGGGCAGAA GGTCCGGCTG
CTGAAGAAAG CTCTGGAGAA GCACGCAGAC AAGGAGGATC TGGTCATTCT CTTCGCAGAC
AGCTATGACG TGCTGTTTGC ATCGGGGCCC CGGGAGCTCC TGAAGAAGTT CCGGCAGGCC
AGGAGCCAGG TGGTCTTCTC TGCTGAGGAG CTCATCTACC CAGACCGCAG GCTGGAGACC
AAGTATCCGG TGGTGTCCGA TGGCAAGAGG TTCCTGGGCT CTGGAGGCTT CATCGGTTAT
GCCCCCAACC TCAGCAAACT GGTGGCCGAG TGGGAGGGCC AGGACAGCGA CAGCGATCAG
CTGTTTTACA CCAAGATCTT CTTGGACCCG GAGAAGAGGG AGCAGATCAA TATCACCCTG
GACCACCGCT GCCGTATCTT CCAGAACCTG GATGGAGCCT TGGATGAGGT CGTGCTCAAG
TTTGAAATGG GCCATGTGAG AGCGAGGAAC CTGGCCTATG ACACCCTCCC GGTCCTGATC
CATGGCAACG GGCCAACCAA GCTGCAGTTG AACTACCTGG GCAACTACAT CCCGCGCTTC
TGGACCTTCG AAACAGGCTG CACCGTGTGT GACGAAGGCT TGCGCAGCCT CAAGGGCATT
GGGGATGAAG CTCTGCCCAC GGTCCTGGTC GGCGTGTTCA TCGAACAGCC CACGCCGTTT
GTGTCCCTGT TCTTCCAGCG GCTCCTGCGG CTCCACTACC CCCAGAAACA CATGCGACTT
TTCATCCACA ACCACGAGCA GCACCACAAG GCTCAGGTGG AAGAGTTCCT GGCACAGCAT
GGCAGCGAGT ACCAGTCTGT GAAGCTGGTG GGCCCTGAGG TGCGGATGGC GAATGCAGAT
GCCAGGAACA TGGGCGCAGA CCTGTGCCGG CAGGACCGCA GCTGCACCTA CTACTTCAGC
GTGGATGCTG ACGTGGCCCT GACCGAGCCC AACAGCCTGC GGCTGCTGAT CCAACAGAAC
AAGAACGTCA TTGCCCCGCT GATGACCCGG CATGGGAGGC TGTGGTCGAA CTTCTGGGGG
GCTCTCAGTG CAGATGGCTA CTATGCCCGT TCCGAGGACT ACGTGGACAT TGTGCAGGGG
CGGCGTGTTG GTGTCTGGAA TGTGCCCTAT ATTTCAAACA TCTACTTGAT CAAGGGCAGT
GCCCTGCGGG GTGAGCTGCA GTCCTCAGAT CTCTTCCACC ACAGCAAGCT GGACCCCGAC
ATGGCCTTCT GTGCCAACAT CCGGCAGCAG GATGTGTTCA TGTTCCTGAC CAACCGGCAC
ACCCTTGGCC ATCTGCTCTC CCTAGACAGC TACCGCACCA CCCACCTGCA CAACGACCTC
TGGGAGGTGT TCAGCAACCC CGAGGACTGG AAGGAGAAGT ACATCCACCA GAACTACACC
AAAGCCCTGG CAGGGAAGCT GGTGGAGACG CCCTGCCCGG ATGTCTATTG GTTCCCCATC
TTCACGGAGG TGGCCTGTGA TGAGCTGGTG GAGGAGATGG AGCACTTTGG CCAGTGGTCT
CTGGGCAACA ACAAGGACAA CCGCATCCAG GGTGGCTACG AGAACGTGCC GACTATTGAC
ATCCACATGA ACCAGATCGG CTTTGAGCGG GAGTGGCACA AATTCCTGCT GGAGTACATT
GCGCCCATGA CGGAGAAGCT CTACCCCGGC TACTACACCA GGGCCCAGTT TGACCTGGCC
TTTGTCGTCC GCTACAAGCC TGATGAGCAG CCCTCACTGA TGCCACACCA TGATGCCTCC
ACCTTCACCA TCAACATCGC CCTGAACCGA GTCGGGGTGG ATTACGAGGG CGGGGGCTGT
CGGTTCCTGC GCTACAACTG TTCCATCCGA GCCCCAAGGA AGGGCTGGAC CCTCATGCAC
CCTGGACGAC TCACGCATTA CCATGAGGGG CTCCCCACCA CCAGGGGCAC CCGCTACATC
GCAGTCTCCT TCGTCGATCC CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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