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PMP22 peripheral myelin protein 22 [Homo sapiens (human)]

Gene Symbol PMP22
Entrez Gene ID 5376
Full Name peripheral myelin protein 22
Synonyms CMT1A, CMT1E, DSS, GAS-3, HMSNIA, HNPP, Sp110
General protein information
Preferred Names
peripheral myelin protein 22
Names
peripheral myelin protein 22
growth arrest-specific protein 3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17p12

Summary This gene encodes an integral membrane protein that is a major component of myelin in the peripheral nervous system. Studies suggest two alternately used promoters drive tissue-specific expression. Various mutations of this gene are causes of Charcot-Marie-Tooth disease Type IA, Dejerine-Sottas syndrome, and hereditary neuropathy with liability to pressure palsies. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]. lac of sum
Disorder MIM:

601097

Disorder Html: Charcot-Marie-Tooth disease, type 1A, 118220 (3); Dejerine-Sottas





The following PMP22 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PMP22 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu59792 XM_011523943 PREDICTED: Homo sapiens peripheral myelin protein 22 (PMP22), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99
OHu16691 NM_000304 Homo sapiens peripheral myelin protein 22 (PMP22), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.3
$99
OHu16691 NM_153321 Homo sapiens peripheral myelin protein 22 (PMP22), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.3
$99
OHu16691 NM_153322 Homo sapiens peripheral myelin protein 22 (PMP22), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.3
$99
OHu16691 NM_001281455 Homo sapiens peripheral myelin protein 22 (PMP22), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.3
$99
OHu16691 NM_001281456 Homo sapiens peripheral myelin protein 22 (PMP22), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.3
$99

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu59792
Accession Version XM_011523943.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 357bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product peripheral myelin protein 22 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010718.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
ATGCTCCTCC TGTTGCTGAG TATCATCGTC CTCCACGTCG CGGTGCTGGT GCTGCTGTTC 
GTCTCCACGA TCGTCAGCCA ATGGATCGTG GGCAATGGAC ACGCAACTGA TCTCTGGCAG
AACTGTAGCA CCTCTTCCTC AGGAAATGTC CACCACTGTT TCTCATCATC ACCAAACGAA
TGGCTGCAGT CTGTCCAGGC CACCATGATC CTGTCGATCA TCTTCAGCAT TCTGTCTCTG
TTCCTGTTCT TCTGCCAACT CTTCACCCTC ACCAAGGGGG GCAGGTTTTA CATCACTGGA
ATCTTCCAAA TTCTTGCTGA ATCAAACTCA AGTCAAATGT GTGGTTACCG GGATTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011522245.1
CDS128..484
Misc Feature(1)191..>445(+)
Translation
Position Chain Variation Link
26 26 -, g dbSNP:35281682
41 41 c, g dbSNP:192617032
68 68 a, g dbSNP:138538875
73 73 c, g dbSNP:557597089
84 84 c, g dbSNP:537267675
101 101 a, c dbSNP:371965531
108 108 a, g dbSNP:533741268
110 110 g, t dbSNP:192086569
118 118 g, t dbSNP:773046723
122 122 g, t dbSNP:369779295
127 127 a, c dbSNP:748020473
145 145 c, g dbSNP:781550490
146 146 -, ag dbSNP:587776691
148 148 c, t dbSNP:755300278
152 152 a, g dbSNP:138515303
163 163 a, c dbSNP:104894622
174 174 c, t dbSNP:104894617
181 181 c, g dbSNP:780138909
192 192 c, t dbSNP:104894625
196 196 c, g dbSNP:376847762
199 199 a, c dbSNP:371373574
201 201 g, t dbSNP:765741053
209 209 c, t dbSNP:104894626
214 214 c, t dbSNP:201192820
215 215 a, c, g dbSNP:377335295
220 220 a, c dbSNP:779382797
222 222 a, g dbSNP:373322590
225 225 a, g dbSNP:754175558
226 226 a, g dbSNP:778072164
227 227 c, t dbSNP:756458019
229 229 a, c, g, t dbSNP:779654897
230 230 a, g dbSNP:750000952
231 231 c, g dbSNP:765235223
237 237 a, t dbSNP:104894627
247 247 g, t dbSNP:761801976
253 253 c, t dbSNP:776731388
255 255 c, g dbSNP:539999617
257 257 a, g, t dbSNP:112651887
279 279 a, g dbSNP:368908933
295 295 a, t dbSNP:771749546
299 299 c, t dbSNP:772242644
300 300 a, c dbSNP:745939923
303 303 a, g dbSNP:779004441
304 304 c, t dbSNP:376797385
311 311 c, t dbSNP:764071801
312 312 g, t dbSNP:756046682
315 315 a, t dbSNP:752712529
322 322 c, t dbSNP:767172855
326 326 a, c, g dbSNP:104894623
333 333 a, t dbSNP:104894620
337 337 c, t dbSNP:759343506
342 342 c, t dbSNP:104894621
343 343 a, g dbSNP:774493881
359 359 a, c dbSNP:766656618
361 361 a, g dbSNP:745546543
363 363 c, g dbSNP:104894618
365 365 -, ct dbSNP:786205087
365 365 c, g dbSNP:773201903
367 367 c, g dbSNP:769945527
368 368 c, t dbSNP:748551014
373 373 a, g dbSNP:779700333
382 382 c, g dbSNP:755701957
383 383 a, c dbSNP:11545341
393 393 a, c dbSNP:189205303
395 395 c, g dbSNP:147114400
402 402 a, g dbSNP:112232836
404 404 a, c, g dbSNP:778693173
405 405 c, g dbSNP:757021177
408 408 -, g dbSNP:80338763
408 408 -, g dbSNP:786205112
409 409 c, t dbSNP:750868826
419 419 a, c dbSNP:748778392
422 422 a, c dbSNP:777109239
435 435 a, g dbSNP:786204064
438 438 a, t dbSNP:755673309
443 443 a, g dbSNP:368223794
445 445 c, t dbSNP:778234032
456 456 c, t dbSNP:748672868
459 459 a, g dbSNP:569346422
476 476 c, t dbSNP:761225505
477 477 a, g dbSNP:549241131
494 494 g, t dbSNP:192174882
503 503 a, g dbSNP:776164058
513 513 g, t dbSNP:763975087
532 532 g, t dbSNP:543972726
534 534 c, t dbSNP:779330487
560 560 c, t dbSNP:560156724
579 579 a, g dbSNP:573809874
626 626 a, g dbSNP:3207088
627 627 c, t dbSNP:769239703
648 648 c, t dbSNP:533449461
651 651 g, t dbSNP:564410916
702 702 c, t dbSNP:62070862
715 715 a, g dbSNP:780484698
760 760 c, g dbSNP:72812942
772 772 c, t dbSNP:575782066
776 776 a, g dbSNP:555907001
825 825 a, g dbSNP:549138856
837 837 a, c dbSNP:230948
848 848 g, t dbSNP:572964331
884 884 a, t dbSNP:553011752

Target ORF information:

RefSeq Version XM_011523943
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens peripheral myelin protein 22 (PMP22), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011523943

ORF Insert Sequence:

1
61
121
181
241
301
ATGCTCCTCC TGTTGCTGAG TATCATCGTC CTCCACGTCG CGGTGCTGGT GCTGCTGTTC 
GTCTCCACGA TCGTCAGCCA ATGGATCGTG GGCAATGGAC ACGCAACTGA TCTCTGGCAG
AACTGTAGCA CCTCTTCCTC AGGAAATGTC CACCACTGTT TCTCATCATC ACCAAACGAA
TGGCTGCAGT CTGTCCAGGC CACCATGATC CTGTCGATCA TCTTCAGCAT TCTGTCTCTG
TTCCTGTTCT TCTGCCAACT CTTCACCCTC ACCAAGGGGG GCAGGTTTTA CATCACTGGA
ATCTTCCAAA TTCTTGCTGA ATCAAACTCA AGTCAAATGT GTGGTTACCG GGATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16691
Accession Version NM_000304.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 483bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product peripheral myelin protein 22
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB504971.1, D11428.1, BQ212717.1, CK818720.1 and DV460945.1. On Jul 27, 2013 this sequence version replaced gi:24430161. Summary: This gene encodes an integral membrane protein that is a major component of myelin in the peripheral nervous system. Studies suggest two alternately used promoters drive tissue-specific expression. Various mutations of this gene are causes of Charcot-Marie-Tooth disease Type IA, Dejerine-Sottas syndrome, and hereditary neuropathy with liability to pressure palsies. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]. Transcript Variant: This variant (1) encodes the functional protein. Variants 1, 2, 3 ,4, and 5 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D11428.1, BC091499.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2162568 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
ATGCTCCTCC TGTTGCTGAG TATCATCGTC CTCCACGTCG CGGTGCTGGT GCTGCTGTTC 
GTCTCCACGA TCGTCAGCCA ATGGATCGTG GGCAATGGAC ACGCAACTGA TCTCTGGCAG
AACTGTAGCA CCTCTTCCTC AGGAAATGTC CACCACTGTT TCTCATCATC ACCAAACGAA
TGGCTGCAGT CTGTCCAGGC CACCATGATC CTGTCGATCA TCTTCAGCAT TCTGTCTCTG
TTCCTGTTCT TCTGCCAACT CTTCACCCTC ACCAAGGGGG GCAGGTTTTA CATCACTGGA
ATCTTCCAAA TTCTTGCTGG TCTGTGCGTG ATGAGTGCTG CGGCCATCTA CACGGTGAGG
CACCCGGAGT GGCATCTCAA CTCGGATTAC TCCTACGGTT TCGCCTACAT CCTGGCCTGG
GTGGCCTTCC CCCTGGCCCT TCTCAGCGGT GTCATCTATG TGATCTTGCG GAAACGCGAA
TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000295.1
CDS239..721
Misc Feature(1)32..32(+)
Misc Feature(2)218..220(+)
Misc Feature(3)239..697(+)
Misc Feature(4)242..331(+)
Misc Feature(5)359..361(+)
Misc Feature(6)431..511(+)
Misc Feature(7)524..595(+)
Misc Feature(8)638..706(+)
Exon (1)1..204
Gene:PMP22
Gene Synonym:
Exon (2)205..316
Gene:PMP22
Gene Synonym:
Exon (3)317..416
Gene:PMP22
Gene Synonym:
Exon (4)417..557
Gene:PMP22
Gene Synonym:
Exon (5)558..1861
Gene:PMP22
Gene Synonym:
Translation
Position Chain Variation Link
16 16 c, t dbSNP:549380262
52 52 a, g dbSNP:143283160
62 62 c, t dbSNP:372385075
98 98 c, g dbSNP:560442424
105 105 a, g dbSNP:754758124
145 145 c, t dbSNP:184477058
153 153 a, c dbSNP:140650630
165 165 a, g dbSNP:114365663
192 192 a, g dbSNP:766242271
202 202 c, t dbSNP:543840509
212 212 a, c dbSNP:371965531
219 219 a, g dbSNP:533741268
221 221 g, t dbSNP:192086569
229 229 g, t dbSNP:773046723
233 233 g, t dbSNP:369779295
238 238 a, c dbSNP:748020473
256 256 c, g dbSNP:781550490
257 257 -, ag dbSNP:587776691
259 259 c, t dbSNP:755300278
263 263 a, g dbSNP:138515303
274 274 a, c dbSNP:104894622
285 285 c, t dbSNP:104894617
292 292 c, g dbSNP:780138909
303 303 c, t dbSNP:104894625
307 307 c, g dbSNP:376847762
310 310 a, c dbSNP:371373574
312 312 g, t dbSNP:765741053
320 320 c, t dbSNP:104894626
325 325 c, t dbSNP:201192820
326 326 a, c, g dbSNP:377335295
331 331 a, c dbSNP:779382797
333 333 a, g dbSNP:373322590
336 336 a, g dbSNP:754175558
337 337 a, g dbSNP:778072164
338 338 c, t dbSNP:756458019
340 340 a, c, g, t dbSNP:779654897
341 341 a, g dbSNP:750000952
342 342 c, g dbSNP:765235223
348 348 a, t dbSNP:104894627
358 358 g, t dbSNP:761801976
364 364 c, t dbSNP:776731388
366 366 c, g dbSNP:539999617
368 368 a, g, t dbSNP:112651887
390 390 a, g dbSNP:368908933
406 406 a, t dbSNP:771749546
410 410 c, t dbSNP:772242644
411 411 a, c dbSNP:745939923
414 414 a, g dbSNP:779004441
415 415 c, t dbSNP:376797385
422 422 c, t dbSNP:764071801
423 423 g, t dbSNP:756046682
426 426 a, t dbSNP:752712529
433 433 c, t dbSNP:767172855
437 437 a, c, g dbSNP:104894623
444 444 a, t dbSNP:104894620
448 448 c, t dbSNP:759343506
453 453 c, t dbSNP:104894621
454 454 a, g dbSNP:774493881
470 470 a, c dbSNP:766656618
472 472 a, g dbSNP:745546543
474 474 c, g dbSNP:104894618
476 476 -, ct dbSNP:786205087
476 476 c, g dbSNP:773201903
478 478 c, g dbSNP:769945527
479 479 c, t dbSNP:748551014
484 484 a, g dbSNP:779700333
493 493 c, g dbSNP:755701957
494 494 a, c dbSNP:11545341
504 504 a, c dbSNP:189205303
506 506 c, g dbSNP:147114400
513 513 a, g dbSNP:112232836
515 515 a, c, g dbSNP:778693173
516 516 c, g dbSNP:757021177
519 519 -, g dbSNP:80338763
519 519 -, g dbSNP:786205112
520 520 c, t dbSNP:750868826
530 530 a, c dbSNP:748778392
533 533 a, c dbSNP:777109239
546 546 a, g dbSNP:786204064
549 549 a, t dbSNP:755673309
554 554 a, g dbSNP:368223794
556 556 c, t dbSNP:778234032
562 562 a, g dbSNP:768210658
570 570 c, t dbSNP:759808649
576 576 c, t dbSNP:774831750
580 580 a, g dbSNP:201871607
582 582 -, ccatctacacgg dbSNP:786205111
582 582 c, t dbSNP:148822354
591 591 c, t dbSNP:104894619
592 592 g, t dbSNP:776275784
603 603 c, t dbSNP:768085434
604 604 a, g, t dbSNP:372723732
607 607 a, g dbSNP:558210039
608 608 a, c, t dbSNP:576618192
619 619 c, t dbSNP:369477577
621 621 c, t dbSNP:778731157
622 622 a, g dbSNP:757027432
634 634 c, t dbSNP:74361095
635 635 a, g dbSNP:140763467
640 640 c, t dbSNP:536417866
641 641 a, g dbSNP:141094419
645 645 a, c dbSNP:375449671
647 647 a, g dbSNP:755551524
649 649 c, t dbSNP:752025670
661 661 g, t dbSNP:144077495
664 664 c, t dbSNP:763227987
667 667 c, t dbSNP:776257683
668 668 a, c dbSNP:201255121
676 676 c, t dbSNP:533618640
685 685 c, t dbSNP:775019409
686 686 g, t dbSNP:104894624
696 696 a, g dbSNP:761138676
704 704 g, t dbSNP:771512763
706 706 a, g dbSNP:745772135
707 707 c, t dbSNP:28936682
709 709 a, g dbSNP:770693814
713 713 c, t dbSNP:749165928
714 714 a, g dbSNP:773478255
715 715 c, t dbSNP:778132801
720 720 a, g dbSNP:770193337
724 724 c, t dbSNP:373690370
725 725 a, g, t dbSNP:570955542
728 728 -, c dbSNP:758935587
729 729 a, t dbSNP:199650994
732 732 a, c, t dbSNP:772282020
733 733 a, g dbSNP:758748518
747 747 c, t dbSNP:200563670
753 753 c, t dbSNP:763703270
754 754 a, g dbSNP:201128796
758 758 a, g dbSNP:752369935
759 759 g, t dbSNP:549180136
780 780 a, c dbSNP:13422
796 796 a, g dbSNP:11545342
811 811 a, c dbSNP:774341981
813 813 a, c dbSNP:560368157
819 819 c, g dbSNP:541586082
823 823 a, g dbSNP:773184749
831 831 c, t dbSNP:769746708
836 836 -, caaac dbSNP:112829799
853 853 a, c, g dbSNP:781273381
857 857 a, g dbSNP:768773339
861 861 a, g dbSNP:747112334
865 865 c, t dbSNP:780587002
872 872 c, t dbSNP:572563714
878 878 a, g dbSNP:750957962
892 892 c, t dbSNP:779198796
894 894 c, t dbSNP:117277951
897 897 c, t dbSNP:752328330
919 919 c, g dbSNP:767142484
921 921 g, t dbSNP:142106420
926 926 a, c dbSNP:189734097
927 927 a, g dbSNP:766390986
930 930 a, t dbSNP:762946632
949 949 a, g dbSNP:1804193
956 956 a, g dbSNP:540148653
964 964 c, t dbSNP:533987307
1041 1041 -, t dbSNP:765955811
1077 1077 g, t dbSNP:373020223
1097 1097 c, t dbSNP:575964802
1140 1140 c, g dbSNP:533679946
1167 1167 c, g dbSNP:746771913
1247 1247 -, t dbSNP:16418
1248 1248 -, ct dbSNP:71699667
1263 1263 c, t dbSNP:184928176
1267 1267 c, t dbSNP:551380652
1271 1271 c, t dbSNP:775055426
1298 1298 c, t dbSNP:7538
1308 1308 c, t dbSNP:568822260
1356 1356 c, t dbSNP:557609991
1381 1381 -, aca dbSNP:371508374
1420 1420 c, t dbSNP:181257692
1485 1485 a, g dbSNP:747872532
1504 1504 a, t dbSNP:375638809
1539 1539 c, t dbSNP:529200588
1542 1542 c, g dbSNP:1803629
1549 1549 a, g dbSNP:13027
1560 1560 -, tta dbSNP:550359128
1589 1589 c, t dbSNP:754583973
1606 1606 c, t dbSNP:1804192
1611 1611 c, t dbSNP:546778557
1616 1616 a, g dbSNP:568790725
1633 1633 a, g dbSNP:149070440
1651 1651 c, t dbSNP:201970368
1652 1652 a, g dbSNP:552027045
1682 1682 c, g dbSNP:760879114
1709 1709 g, t dbSNP:545400789
1715 1715 a, c dbSNP:756594808
1748 1748 a, t dbSNP:189748556
1754 1754 c, g dbSNP:562876551
1770 1770 c, g dbSNP:750733887
1832 1832 g, t dbSNP:7415
1841 1841 c, t dbSNP:11654383

Target ORF information:

RefSeq Version NM_000304
Organism Homo sapiens (human)
Definition Homo sapiens peripheral myelin protein 22 (PMP22), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000304

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCTCCTCC TGTTGCTGAG TATCATCGTC CTCCACGTCG CGGTGCTGGT GCTGCTGTTC 
GTCTCCACGA TCGTCAGCCA ATGGATCGTG GGCAATGGAC ACGCAACTGA TCTCTGGCAG
AACTGTAGCA CCTCTTCCTC AGGAAATGTC CACCACTGTT TCTCATCATC ACCAAACGAA
TGGCTGCAGT CTGTCCAGGC CACCATGATC CTGTCGATCA TCTTCAGCAT TCTGTCTCTG
TTCCTGTTCT TCTGCCAACT CTTCACCCTC ACCAAGGGGG GCAGGTTTTA CATCACTGGA
ATCTTCCAAA TTCTTGCTGG TCTGTGCGTG ATGAGTGCTG CGGCCATCTA CACGGTGAGG
CACCCGGAGT GGCATCTCAA CTCGGATTAC TCCTACGGTT TCGCCTACAT CCTGGCCTGG
GTGGCCTTCC CCCTGGCCCT TCTCAGCGGT GTCATCTATG TGATCTTGCG GAAACGCGAA
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16691
Accession Version NM_153321.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 483bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product peripheral myelin protein 22
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX464118.2, BC019040.2 and DV460945.1. On Jul 27, 2013 this sequence version replaced gi:24430162. Summary: This gene encodes an integral membrane protein that is a major component of myelin in the peripheral nervous system. Studies suggest two alternately used promoters drive tissue-specific expression. Various mutations of this gene are causes of Charcot-Marie-Tooth disease Type IA, Dejerine-Sottas syndrome, and hereditary neuropathy with liability to pressure palsies. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3 ,4, and 5 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL539407.3, BC019040.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2162568 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
ATGCTCCTCC TGTTGCTGAG TATCATCGTC CTCCACGTCG CGGTGCTGGT GCTGCTGTTC 
GTCTCCACGA TCGTCAGCCA ATGGATCGTG GGCAATGGAC ACGCAACTGA TCTCTGGCAG
AACTGTAGCA CCTCTTCCTC AGGAAATGTC CACCACTGTT TCTCATCATC ACCAAACGAA
TGGCTGCAGT CTGTCCAGGC CACCATGATC CTGTCGATCA TCTTCAGCAT TCTGTCTCTG
TTCCTGTTCT TCTGCCAACT CTTCACCCTC ACCAAGGGGG GCAGGTTTTA CATCACTGGA
ATCTTCCAAA TTCTTGCTGG TCTGTGCGTG ATGAGTGCTG CGGCCATCTA CACGGTGAGG
CACCCGGAGT GGCATCTCAA CTCGGATTAC TCCTACGGTT TCGCCTACAT CCTGGCCTGG
GTGGCCTTCC CCCTGGCCCT TCTCAGCGGT GTCATCTATG TGATCTTGCG GAAACGCGAA
TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_696996.1
CDS196..678
Misc Feature(1)18..18(+)
Misc Feature(2)175..177(+)
Misc Feature(3)196..654(+)
Misc Feature(4)199..288(+)
Misc Feature(5)316..318(+)
Misc Feature(6)388..468(+)
Misc Feature(7)481..552(+)
Misc Feature(8)595..663(+)
Exon (1)1..161
Gene:PMP22
Gene Synonym:
Exon (2)162..273
Gene:PMP22
Gene Synonym:
Exon (3)274..373
Gene:PMP22
Gene Synonym:
Exon (4)374..514
Gene:PMP22
Gene Synonym:
Exon (5)515..1818
Gene:PMP22
Gene Synonym:
Translation
Position Chain Variation Link
7 7 c, t dbSNP:368241011
18 18 a, g dbSNP:552996334
94 94 -, g dbSNP:35281682
109 109 c, g dbSNP:192617032
136 136 a, g dbSNP:138538875
141 141 c, g dbSNP:557597089
152 152 c, g dbSNP:537267675
169 169 a, c dbSNP:371965531
176 176 a, g dbSNP:533741268
178 178 g, t dbSNP:192086569
186 186 g, t dbSNP:773046723
190 190 g, t dbSNP:369779295
195 195 a, c dbSNP:748020473
213 213 c, g dbSNP:781550490
214 214 -, ag dbSNP:587776691
216 216 c, t dbSNP:755300278
220 220 a, g dbSNP:138515303
231 231 a, c dbSNP:104894622
242 242 c, t dbSNP:104894617
249 249 c, g dbSNP:780138909
260 260 c, t dbSNP:104894625
264 264 c, g dbSNP:376847762
267 267 a, c dbSNP:371373574
269 269 g, t dbSNP:765741053
277 277 c, t dbSNP:104894626
282 282 c, t dbSNP:201192820
283 283 a, c, g dbSNP:377335295
288 288 a, c dbSNP:779382797
290 290 a, g dbSNP:373322590
293 293 a, g dbSNP:754175558
294 294 a, g dbSNP:778072164
295 295 c, t dbSNP:756458019
297 297 a, c, g, t dbSNP:779654897
298 298 a, g dbSNP:750000952
299 299 c, g dbSNP:765235223
305 305 a, t dbSNP:104894627
315 315 g, t dbSNP:761801976
321 321 c, t dbSNP:776731388
323 323 c, g dbSNP:539999617
325 325 a, g, t dbSNP:112651887
347 347 a, g dbSNP:368908933
363 363 a, t dbSNP:771749546
367 367 c, t dbSNP:772242644
368 368 a, c dbSNP:745939923
371 371 a, g dbSNP:779004441
372 372 c, t dbSNP:376797385
379 379 c, t dbSNP:764071801
380 380 g, t dbSNP:756046682
383 383 a, t dbSNP:752712529
390 390 c, t dbSNP:767172855
394 394 a, c, g dbSNP:104894623
401 401 a, t dbSNP:104894620
405 405 c, t dbSNP:759343506
410 410 c, t dbSNP:104894621
411 411 a, g dbSNP:774493881
427 427 a, c dbSNP:766656618
429 429 a, g dbSNP:745546543
431 431 c, g dbSNP:104894618
433 433 -, ct dbSNP:786205087
433 433 c, g dbSNP:773201903
435 435 c, g dbSNP:769945527
436 436 c, t dbSNP:748551014
441 441 a, g dbSNP:779700333
450 450 c, g dbSNP:755701957
451 451 a, c dbSNP:11545341
461 461 a, c dbSNP:189205303
463 463 c, g dbSNP:147114400
470 470 a, g dbSNP:112232836
472 472 a, c, g dbSNP:778693173
473 473 c, g dbSNP:757021177
476 476 -, g dbSNP:80338763
476 476 -, g dbSNP:786205112
477 477 c, t dbSNP:750868826
487 487 a, c dbSNP:748778392
490 490 a, c dbSNP:777109239
503 503 a, g dbSNP:786204064
506 506 a, t dbSNP:755673309
511 511 a, g dbSNP:368223794
513 513 c, t dbSNP:778234032
519 519 a, g dbSNP:768210658
527 527 c, t dbSNP:759808649
533 533 c, t dbSNP:774831750
537 537 a, g dbSNP:201871607
539 539 -, ccatctacacgg dbSNP:786205111
539 539 c, t dbSNP:148822354
548 548 c, t dbSNP:104894619
549 549 g, t dbSNP:776275784
560 560 c, t dbSNP:768085434
561 561 a, g, t dbSNP:372723732
564 564 a, g dbSNP:558210039
565 565 a, c, t dbSNP:576618192
576 576 c, t dbSNP:369477577
578 578 c, t dbSNP:778731157
579 579 a, g dbSNP:757027432
591 591 c, t dbSNP:74361095
592 592 a, g dbSNP:140763467
597 597 c, t dbSNP:536417866
598 598 a, g dbSNP:141094419
602 602 a, c dbSNP:375449671
604 604 a, g dbSNP:755551524
606 606 c, t dbSNP:752025670
618 618 g, t dbSNP:144077495
621 621 c, t dbSNP:763227987
624 624 c, t dbSNP:776257683
625 625 a, c dbSNP:201255121
633 633 c, t dbSNP:533618640
642 642 c, t dbSNP:775019409
643 643 g, t dbSNP:104894624
653 653 a, g dbSNP:761138676
661 661 g, t dbSNP:771512763
663 663 a, g dbSNP:745772135
664 664 c, t dbSNP:28936682
666 666 a, g dbSNP:770693814
670 670 c, t dbSNP:749165928
671 671 a, g dbSNP:773478255
672 672 c, t dbSNP:778132801
677 677 a, g dbSNP:770193337
681 681 c, t dbSNP:373690370
682 682 a, g, t dbSNP:570955542
685 685 -, c dbSNP:758935587
686 686 a, t dbSNP:199650994
689 689 a, c, t dbSNP:772282020
690 690 a, g dbSNP:758748518
704 704 c, t dbSNP:200563670
710 710 c, t dbSNP:763703270
711 711 a, g dbSNP:201128796
715 715 a, g dbSNP:752369935
716 716 g, t dbSNP:549180136
737 737 a, c dbSNP:13422
753 753 a, g dbSNP:11545342
768 768 a, c dbSNP:774341981
770 770 a, c dbSNP:560368157
776 776 c, g dbSNP:541586082
780 780 a, g dbSNP:773184749
788 788 c, t dbSNP:769746708
793 793 -, caaac dbSNP:112829799
810 810 a, c, g dbSNP:781273381
814 814 a, g dbSNP:768773339
818 818 a, g dbSNP:747112334
822 822 c, t dbSNP:780587002
829 829 c, t dbSNP:572563714
835 835 a, g dbSNP:750957962
849 849 c, t dbSNP:779198796
851 851 c, t dbSNP:117277951
854 854 c, t dbSNP:752328330
876 876 c, g dbSNP:767142484
878 878 g, t dbSNP:142106420
883 883 a, c dbSNP:189734097
884 884 a, g dbSNP:766390986
887 887 a, t dbSNP:762946632
906 906 a, g dbSNP:1804193
913 913 a, g dbSNP:540148653
921 921 c, t dbSNP:533987307
998 998 -, t dbSNP:765955811
1034 1034 g, t dbSNP:373020223
1054 1054 c, t dbSNP:575964802
1097 1097 c, g dbSNP:533679946
1124 1124 c, g dbSNP:746771913
1204 1204 -, t dbSNP:16418
1205 1205 -, ct dbSNP:71699667
1220 1220 c, t dbSNP:184928176
1224 1224 c, t dbSNP:551380652
1228 1228 c, t dbSNP:775055426
1255 1255 c, t dbSNP:7538
1265 1265 c, t dbSNP:568822260
1313 1313 c, t dbSNP:557609991
1338 1338 -, aca dbSNP:371508374
1377 1377 c, t dbSNP:181257692
1442 1442 a, g dbSNP:747872532
1461 1461 a, t dbSNP:375638809
1496 1496 c, t dbSNP:529200588
1499 1499 c, g dbSNP:1803629
1506 1506 a, g dbSNP:13027
1517 1517 -, tta dbSNP:550359128
1546 1546 c, t dbSNP:754583973
1563 1563 c, t dbSNP:1804192
1568 1568 c, t dbSNP:546778557
1573 1573 a, g dbSNP:568790725
1590 1590 a, g dbSNP:149070440
1608 1608 c, t dbSNP:201970368
1609 1609 a, g dbSNP:552027045
1639 1639 c, g dbSNP:760879114
1666 1666 g, t dbSNP:545400789
1672 1672 a, c dbSNP:756594808
1705 1705 a, t dbSNP:189748556
1711 1711 c, g dbSNP:562876551
1727 1727 c, g dbSNP:750733887
1789 1789 g, t dbSNP:7415
1798 1798 c, t dbSNP:11654383

Target ORF information:

RefSeq Version NM_153321
Organism Homo sapiens (human)
Definition Homo sapiens peripheral myelin protein 22 (PMP22), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_153321

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCTCCTCC TGTTGCTGAG TATCATCGTC CTCCACGTCG CGGTGCTGGT GCTGCTGTTC 
GTCTCCACGA TCGTCAGCCA ATGGATCGTG GGCAATGGAC ACGCAACTGA TCTCTGGCAG
AACTGTAGCA CCTCTTCCTC AGGAAATGTC CACCACTGTT TCTCATCATC ACCAAACGAA
TGGCTGCAGT CTGTCCAGGC CACCATGATC CTGTCGATCA TCTTCAGCAT TCTGTCTCTG
TTCCTGTTCT TCTGCCAACT CTTCACCCTC ACCAAGGGGG GCAGGTTTTA CATCACTGGA
ATCTTCCAAA TTCTTGCTGG TCTGTGCGTG ATGAGTGCTG CGGCCATCTA CACGGTGAGG
CACCCGGAGT GGCATCTCAA CTCGGATTAC TCCTACGGTT TCGCCTACAT CCTGGCCTGG
GTGGCCTTCC CCCTGGCCCT TCTCAGCGGT GTCATCTATG TGATCTTGCG GAAACGCGAA
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16691
Accession Version NM_153322.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 483bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product peripheral myelin protein 22
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY119295.1, M94048.1, BG424796.1 and DV460945.1. On Jul 27, 2013 this sequence version replaced gi:24430164. Summary: This gene encodes an integral membrane protein that is a major component of myelin in the peripheral nervous system. Studies suggest two alternately used promoters drive tissue-specific expression. Various mutations of this gene are causes of Charcot-Marie-Tooth disease Type IA, Dejerine-Sottas syndrome, and hereditary neuropathy with liability to pressure palsies. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3 ,4, and 5 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY119295.1, M94048.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2162568 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGCTCCTCC TGTTGCTGAG TATCATCGTC CTCCACGTCG CGGTGCTGGT GCTGCTGTTC 
GTCTCCACGA TCGTCAGCCA ATGGATCGTG GGCAATGGAC ACGCAACTGA TCTCTGGCAG
AACTGTAGCA CCTCTTCCTC AGGAAATGTC CACCACTGTT TCTCATCATC ACCAAACGAA
TGGCTGCAGT CTGTCCAGGC CACCATGATC CTGTCGATCA TCTTCAGCAT TCTGTCTCTG
TTCCTGTTCT TCTGCCAACT CTTCACCCTC ACCAAGGGGG GCAGGTTTTA CATCACTGGA
ATCTTCCAAA TTCTTGCTGG TCTGTGCGTG ATGAGTGCTG CGGCCATCTA CACGGTGAGG
CACCCGGAGT GGCATCTCAA CTCGGATTAC TCCTACGGTT TCGCCTACAT CCTGGCCTGG
GTGGCCTTCC CCCTGGCCCT TCTCAGCGGT GTCATCTATG TGATCTTGCG GAAACGCGAA
TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_696997.1
CDS118..600
Misc Feature(1)97..99(+)
Misc Feature(2)118..576(+)
Misc Feature(3)121..210(+)
Misc Feature(4)238..240(+)
Misc Feature(5)310..390(+)
Misc Feature(6)403..474(+)
Misc Feature(7)517..585(+)
Exon (1)1..195
Gene:PMP22
Gene Synonym:
Exon (2)196..295
Gene:PMP22
Gene Synonym:
Exon (3)296..436
Gene:PMP22
Gene Synonym:
Exon (4)437..1740
Gene:PMP22
Gene Synonym:
Translation
Position Chain Variation Link
8 8 c, g dbSNP:576048994
28 28 a, c, g dbSNP:562778933
43 43 a, g dbSNP:535854111
49 49 c, t dbSNP:567565233
59 59 c, t dbSNP:181535380
67 67 c, t dbSNP:773485031
74 74 c, t dbSNP:778453922
76 76 a, c dbSNP:773965221
79 79 c, t dbSNP:375105159
91 91 a, c dbSNP:371965531
98 98 a, g dbSNP:533741268
100 100 g, t dbSNP:192086569
108 108 g, t dbSNP:773046723
112 112 g, t dbSNP:369779295
117 117 a, c dbSNP:748020473
135 135 c, g dbSNP:781550490
136 136 -, ag dbSNP:587776691
138 138 c, t dbSNP:755300278
142 142 a, g dbSNP:138515303
153 153 a, c dbSNP:104894622
164 164 c, t dbSNP:104894617
171 171 c, g dbSNP:780138909
182 182 c, t dbSNP:104894625
186 186 c, g dbSNP:376847762
189 189 a, c dbSNP:371373574
191 191 g, t dbSNP:765741053
199 199 c, t dbSNP:104894626
204 204 c, t dbSNP:201192820
205 205 a, c, g dbSNP:377335295
210 210 a, c dbSNP:779382797
212 212 a, g dbSNP:373322590
215 215 a, g dbSNP:754175558
216 216 a, g dbSNP:778072164
217 217 c, t dbSNP:756458019
219 219 a, c, g, t dbSNP:779654897
220 220 a, g dbSNP:750000952
221 221 c, g dbSNP:765235223
227 227 a, t dbSNP:104894627
237 237 g, t dbSNP:761801976
243 243 c, t dbSNP:776731388
245 245 c, g dbSNP:539999617
247 247 a, g, t dbSNP:112651887
269 269 a, g dbSNP:368908933
285 285 a, t dbSNP:771749546
289 289 c, t dbSNP:772242644
290 290 a, c dbSNP:745939923
293 293 a, g dbSNP:779004441
294 294 c, t dbSNP:376797385
301 301 c, t dbSNP:764071801
302 302 g, t dbSNP:756046682
305 305 a, t dbSNP:752712529
312 312 c, t dbSNP:767172855
316 316 a, c, g dbSNP:104894623
323 323 a, t dbSNP:104894620
327 327 c, t dbSNP:759343506
332 332 c, t dbSNP:104894621
333 333 a, g dbSNP:774493881
349 349 a, c dbSNP:766656618
351 351 a, g dbSNP:745546543
353 353 c, g dbSNP:104894618
355 355 -, ct dbSNP:786205087
355 355 c, g dbSNP:773201903
357 357 c, g dbSNP:769945527
358 358 c, t dbSNP:748551014
363 363 a, g dbSNP:779700333
372 372 c, g dbSNP:755701957
373 373 a, c dbSNP:11545341
383 383 a, c dbSNP:189205303
385 385 c, g dbSNP:147114400
392 392 a, g dbSNP:112232836
394 394 a, c, g dbSNP:778693173
395 395 c, g dbSNP:757021177
398 398 -, g dbSNP:80338763
398 398 -, g dbSNP:786205112
399 399 c, t dbSNP:750868826
409 409 a, c dbSNP:748778392
412 412 a, c dbSNP:777109239
425 425 a, g dbSNP:786204064
428 428 a, t dbSNP:755673309
433 433 a, g dbSNP:368223794
435 435 c, t dbSNP:778234032
441 441 a, g dbSNP:768210658
449 449 c, t dbSNP:759808649
455 455 c, t dbSNP:774831750
459 459 a, g dbSNP:201871607
461 461 -, ccatctacacgg dbSNP:786205111
461 461 c, t dbSNP:148822354
470 470 c, t dbSNP:104894619
471 471 g, t dbSNP:776275784
482 482 c, t dbSNP:768085434
483 483 a, g, t dbSNP:372723732
486 486 a, g dbSNP:558210039
487 487 a, c, t dbSNP:576618192
498 498 c, t dbSNP:369477577
500 500 c, t dbSNP:778731157
501 501 a, g dbSNP:757027432
513 513 c, t dbSNP:74361095
514 514 a, g dbSNP:140763467
519 519 c, t dbSNP:536417866
520 520 a, g dbSNP:141094419
524 524 a, c dbSNP:375449671
526 526 a, g dbSNP:755551524
528 528 c, t dbSNP:752025670
540 540 g, t dbSNP:144077495
543 543 c, t dbSNP:763227987
546 546 c, t dbSNP:776257683
547 547 a, c dbSNP:201255121
555 555 c, t dbSNP:533618640
564 564 c, t dbSNP:775019409
565 565 g, t dbSNP:104894624
575 575 a, g dbSNP:761138676
583 583 g, t dbSNP:771512763
585 585 a, g dbSNP:745772135
586 586 c, t dbSNP:28936682
588 588 a, g dbSNP:770693814
592 592 c, t dbSNP:749165928
593 593 a, g dbSNP:773478255
594 594 c, t dbSNP:778132801
599 599 a, g dbSNP:770193337
603 603 c, t dbSNP:373690370
604 604 a, g, t dbSNP:570955542
607 607 -, c dbSNP:758935587
608 608 a, t dbSNP:199650994
611 611 a, c, t dbSNP:772282020
612 612 a, g dbSNP:758748518
626 626 c, t dbSNP:200563670
632 632 c, t dbSNP:763703270
633 633 a, g dbSNP:201128796
637 637 a, g dbSNP:752369935
638 638 g, t dbSNP:549180136
659 659 a, c dbSNP:13422
675 675 a, g dbSNP:11545342
690 690 a, c dbSNP:774341981
692 692 a, c dbSNP:560368157
698 698 c, g dbSNP:541586082
702 702 a, g dbSNP:773184749
710 710 c, t dbSNP:769746708
715 715 -, caaac dbSNP:112829799
732 732 a, c, g dbSNP:781273381
736 736 a, g dbSNP:768773339
740 740 a, g dbSNP:747112334
744 744 c, t dbSNP:780587002
751 751 c, t dbSNP:572563714
757 757 a, g dbSNP:750957962
771 771 c, t dbSNP:779198796
773 773 c, t dbSNP:117277951
776 776 c, t dbSNP:752328330
798 798 c, g dbSNP:767142484
800 800 g, t dbSNP:142106420
805 805 a, c dbSNP:189734097
806 806 a, g dbSNP:766390986
809 809 a, t dbSNP:762946632
828 828 a, g dbSNP:1804193
835 835 a, g dbSNP:540148653
843 843 c, t dbSNP:533987307
920 920 -, t dbSNP:765955811
956 956 g, t dbSNP:373020223
976 976 c, t dbSNP:575964802
1019 1019 c, g dbSNP:533679946
1046 1046 c, g dbSNP:746771913
1126 1126 -, t dbSNP:16418
1127 1127 -, ct dbSNP:71699667
1142 1142 c, t dbSNP:184928176
1146 1146 c, t dbSNP:551380652
1150 1150 c, t dbSNP:775055426
1177 1177 c, t dbSNP:7538
1187 1187 c, t dbSNP:568822260
1235 1235 c, t dbSNP:557609991
1260 1260 -, aca dbSNP:371508374
1299 1299 c, t dbSNP:181257692
1364 1364 a, g dbSNP:747872532
1383 1383 a, t dbSNP:375638809
1418 1418 c, t dbSNP:529200588
1421 1421 c, g dbSNP:1803629
1428 1428 a, g dbSNP:13027
1439 1439 -, tta dbSNP:550359128
1468 1468 c, t dbSNP:754583973
1485 1485 c, t dbSNP:1804192
1490 1490 c, t dbSNP:546778557
1495 1495 a, g dbSNP:568790725
1512 1512 a, g dbSNP:149070440
1530 1530 c, t dbSNP:201970368
1531 1531 a, g dbSNP:552027045
1561 1561 c, g dbSNP:760879114
1588 1588 g, t dbSNP:545400789
1594 1594 a, c dbSNP:756594808
1627 1627 a, t dbSNP:189748556
1633 1633 c, g dbSNP:562876551
1649 1649 c, g dbSNP:750733887
1711 1711 g, t dbSNP:7415
1720 1720 c, t dbSNP:11654383

Target ORF information:

RefSeq Version NM_153322
Organism Homo sapiens (human)
Definition Homo sapiens peripheral myelin protein 22 (PMP22), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_153322

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCTCCTCC TGTTGCTGAG TATCATCGTC CTCCACGTCG CGGTGCTGGT GCTGCTGTTC 
GTCTCCACGA TCGTCAGCCA ATGGATCGTG GGCAATGGAC ACGCAACTGA TCTCTGGCAG
AACTGTAGCA CCTCTTCCTC AGGAAATGTC CACCACTGTT TCTCATCATC ACCAAACGAA
TGGCTGCAGT CTGTCCAGGC CACCATGATC CTGTCGATCA TCTTCAGCAT TCTGTCTCTG
TTCCTGTTCT TCTGCCAACT CTTCACCCTC ACCAAGGGGG GCAGGTTTTA CATCACTGGA
ATCTTCCAAA TTCTTGCTGG TCTGTGCGTG ATGAGTGCTG CGGCCATCTA CACGGTGAGG
CACCCGGAGT GGCATCTCAA CTCGGATTAC TCCTACGGTT TCGCCTACAT CCTGGCCTGG
GTGGCCTTCC CCCTGGCCCT TCTCAGCGGT GTCATCTATG TGATCTTGCG GAAACGCGAA
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16691
Accession Version NM_001281455.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 483bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product peripheral myelin protein 22
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX464118.2, BF725606.1, CD519144.1, BQ212717.1, CK818720.1 and DV460945.1. Summary: This gene encodes an integral membrane protein that is a major component of myelin in the peripheral nervous system. Studies suggest two alternately used promoters drive tissue-specific expression. Various mutations of this gene are causes of Charcot-Marie-Tooth disease Type IA, Dejerine-Sottas syndrome, and hereditary neuropathy with liability to pressure palsies. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]. Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3 ,4, and 5 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BF725606.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2145743, SAMEA2162568 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
ATGCTCCTCC TGTTGCTGAG TATCATCGTC CTCCACGTCG CGGTGCTGGT GCTGCTGTTC 
GTCTCCACGA TCGTCAGCCA ATGGATCGTG GGCAATGGAC ACGCAACTGA TCTCTGGCAG
AACTGTAGCA CCTCTTCCTC AGGAAATGTC CACCACTGTT TCTCATCATC ACCAAACGAA
TGGCTGCAGT CTGTCCAGGC CACCATGATC CTGTCGATCA TCTTCAGCAT TCTGTCTCTG
TTCCTGTTCT TCTGCCAACT CTTCACCCTC ACCAAGGGGG GCAGGTTTTA CATCACTGGA
ATCTTCCAAA TTCTTGCTGG TCTGTGCGTG ATGAGTGCTG CGGCCATCTA CACGGTGAGG
CACCCGGAGT GGCATCTCAA CTCGGATTAC TCCTACGGTT TCGCCTACAT CCTGGCCTGG
GTGGCCTTCC CCCTGGCCCT TCTCAGCGGT GTCATCTATG TGATCTTGCG GAAACGCGAA
TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001268384.1
CDS200..682
Misc Feature(1)18..18(+)
Misc Feature(2)179..181(+)
Misc Feature(3)200..658(+)
Misc Feature(4)203..292(+)
Misc Feature(5)392..472(+)
Misc Feature(6)485..556(+)
Misc Feature(7)599..667(+)
Exon (1)1..165
Gene:PMP22
Gene Synonym:
Exon (2)166..277
Gene:PMP22
Gene Synonym:
Exon (3)278..377
Gene:PMP22
Gene Synonym:
Exon (4)378..518
Gene:PMP22
Gene Synonym:
Exon (5)519..1822
Gene:PMP22
Gene Synonym:
Translation
Position Chain Variation Link
7 7 c, t dbSNP:368241011
18 18 a, g dbSNP:552996334
94 94 -, g dbSNP:35281682
109 109 c, g dbSNP:192617032
136 136 a, g dbSNP:138538875
141 141 c, g dbSNP:557597089
152 152 c, g dbSNP:537267675
163 163 c, t dbSNP:111410548
173 173 a, c dbSNP:371965531
180 180 a, g dbSNP:533741268
182 182 g, t dbSNP:192086569
190 190 g, t dbSNP:773046723
194 194 g, t dbSNP:369779295
199 199 a, c dbSNP:748020473
217 217 c, g dbSNP:781550490
218 218 -, ag dbSNP:587776691
220 220 c, t dbSNP:755300278
224 224 a, g dbSNP:138515303
235 235 a, c dbSNP:104894622
246 246 c, t dbSNP:104894617
253 253 c, g dbSNP:780138909
264 264 c, t dbSNP:104894625
268 268 c, g dbSNP:376847762
271 271 a, c dbSNP:371373574
273 273 g, t dbSNP:765741053
281 281 c, t dbSNP:104894626
286 286 c, t dbSNP:201192820
287 287 a, c, g dbSNP:377335295
292 292 a, c dbSNP:779382797
294 294 a, g dbSNP:373322590
297 297 a, g dbSNP:754175558
298 298 a, g dbSNP:778072164
299 299 c, t dbSNP:756458019
301 301 a, c, g, t dbSNP:779654897
302 302 a, g dbSNP:750000952
303 303 c, g dbSNP:765235223
309 309 a, t dbSNP:104894627
319 319 g, t dbSNP:761801976
325 325 c, t dbSNP:776731388
327 327 c, g dbSNP:539999617
329 329 a, g, t dbSNP:112651887
351 351 a, g dbSNP:368908933
367 367 a, t dbSNP:771749546
371 371 c, t dbSNP:772242644
372 372 a, c dbSNP:745939923
375 375 a, g dbSNP:779004441
376 376 c, t dbSNP:376797385
383 383 c, t dbSNP:764071801
384 384 g, t dbSNP:756046682
387 387 a, t dbSNP:752712529
394 394 c, t dbSNP:767172855
398 398 a, c, g dbSNP:104894623
405 405 a, t dbSNP:104894620
409 409 c, t dbSNP:759343506
414 414 c, t dbSNP:104894621
415 415 a, g dbSNP:774493881
431 431 a, c dbSNP:766656618
433 433 a, g dbSNP:745546543
435 435 c, g dbSNP:104894618
437 437 -, ct dbSNP:786205087
437 437 c, g dbSNP:773201903
439 439 c, g dbSNP:769945527
440 440 c, t dbSNP:748551014
445 445 a, g dbSNP:779700333
454 454 c, g dbSNP:755701957
455 455 a, c dbSNP:11545341
465 465 a, c dbSNP:189205303
467 467 c, g dbSNP:147114400
474 474 a, g dbSNP:112232836
476 476 a, c, g dbSNP:778693173
477 477 c, g dbSNP:757021177
480 480 -, g dbSNP:80338763
480 480 -, g dbSNP:786205112
481 481 c, t dbSNP:750868826
491 491 a, c dbSNP:748778392
494 494 a, c dbSNP:777109239
507 507 a, g dbSNP:786204064
510 510 a, t dbSNP:755673309
515 515 a, g dbSNP:368223794
517 517 c, t dbSNP:778234032
523 523 a, g dbSNP:768210658
531 531 c, t dbSNP:759808649
537 537 c, t dbSNP:774831750
541 541 a, g dbSNP:201871607
543 543 -, ccatctacacgg dbSNP:786205111
543 543 c, t dbSNP:148822354
552 552 c, t dbSNP:104894619
553 553 g, t dbSNP:776275784
564 564 c, t dbSNP:768085434
565 565 a, g, t dbSNP:372723732
568 568 a, g dbSNP:558210039
569 569 a, c, t dbSNP:576618192
580 580 c, t dbSNP:369477577
582 582 c, t dbSNP:778731157
583 583 a, g dbSNP:757027432
595 595 c, t dbSNP:74361095
596 596 a, g dbSNP:140763467
601 601 c, t dbSNP:536417866
602 602 a, g dbSNP:141094419
606 606 a, c dbSNP:375449671
608 608 a, g dbSNP:755551524
610 610 c, t dbSNP:752025670
622 622 g, t dbSNP:144077495
625 625 c, t dbSNP:763227987
628 628 c, t dbSNP:776257683
629 629 a, c dbSNP:201255121
637 637 c, t dbSNP:533618640
646 646 c, t dbSNP:775019409
647 647 g, t dbSNP:104894624
657 657 a, g dbSNP:761138676
665 665 g, t dbSNP:771512763
667 667 a, g dbSNP:745772135
668 668 c, t dbSNP:28936682
670 670 a, g dbSNP:770693814
674 674 c, t dbSNP:749165928
675 675 a, g dbSNP:773478255
676 676 c, t dbSNP:778132801
681 681 a, g dbSNP:770193337
685 685 c, t dbSNP:373690370
686 686 a, g, t dbSNP:570955542
689 689 -, c dbSNP:758935587
690 690 a, t dbSNP:199650994
693 693 a, c, t dbSNP:772282020
694 694 a, g dbSNP:758748518
708 708 c, t dbSNP:200563670
714 714 c, t dbSNP:763703270
715 715 a, g dbSNP:201128796
719 719 a, g dbSNP:752369935
720 720 g, t dbSNP:549180136
741 741 a, c dbSNP:13422
757 757 a, g dbSNP:11545342
772 772 a, c dbSNP:774341981
774 774 a, c dbSNP:560368157
780 780 c, g dbSNP:541586082
784 784 a, g dbSNP:773184749
792 792 c, t dbSNP:769746708
797 797 -, caaac dbSNP:112829799
814 814 a, c, g dbSNP:781273381
818 818 a, g dbSNP:768773339
822 822 a, g dbSNP:747112334
826 826 c, t dbSNP:780587002
833 833 c, t dbSNP:572563714
839 839 a, g dbSNP:750957962
853 853 c, t dbSNP:779198796
855 855 c, t dbSNP:117277951
858 858 c, t dbSNP:752328330
880 880 c, g dbSNP:767142484
882 882 g, t dbSNP:142106420
887 887 a, c dbSNP:189734097
888 888 a, g dbSNP:766390986
891 891 a, t dbSNP:762946632
910 910 a, g dbSNP:1804193
917 917 a, g dbSNP:540148653
925 925 c, t dbSNP:533987307
1002 1002 -, t dbSNP:765955811
1038 1038 g, t dbSNP:373020223
1058 1058 c, t dbSNP:575964802
1101 1101 c, g dbSNP:533679946
1128 1128 c, g dbSNP:746771913
1208 1208 -, t dbSNP:16418
1209 1209 -, ct dbSNP:71699667
1224 1224 c, t dbSNP:184928176
1228 1228 c, t dbSNP:551380652
1232 1232 c, t dbSNP:775055426
1259 1259 c, t dbSNP:7538
1269 1269 c, t dbSNP:568822260
1317 1317 c, t dbSNP:557609991
1342 1342 -, aca dbSNP:371508374
1381 1381 c, t dbSNP:181257692
1446 1446 a, g dbSNP:747872532
1465 1465 a, t dbSNP:375638809
1500 1500 c, t dbSNP:529200588
1503 1503 c, g dbSNP:1803629
1510 1510 a, g dbSNP:13027
1521 1521 -, tta dbSNP:550359128
1550 1550 c, t dbSNP:754583973
1567 1567 c, t dbSNP:1804192
1572 1572 c, t dbSNP:546778557
1577 1577 a, g dbSNP:568790725
1594 1594 a, g dbSNP:149070440
1612 1612 c, t dbSNP:201970368
1613 1613 a, g dbSNP:552027045
1643 1643 c, g dbSNP:760879114
1670 1670 g, t dbSNP:545400789
1676 1676 a, c dbSNP:756594808
1709 1709 a, t dbSNP:189748556
1715 1715 c, g dbSNP:562876551
1731 1731 c, g dbSNP:750733887
1793 1793 g, t dbSNP:7415
1802 1802 c, t dbSNP:11654383

Target ORF information:

RefSeq Version NM_001281455
Organism Homo sapiens (human)
Definition Homo sapiens peripheral myelin protein 22 (PMP22), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001281455

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCTCCTCC TGTTGCTGAG TATCATCGTC CTCCACGTCG CGGTGCTGGT GCTGCTGTTC 
GTCTCCACGA TCGTCAGCCA ATGGATCGTG GGCAATGGAC ACGCAACTGA TCTCTGGCAG
AACTGTAGCA CCTCTTCCTC AGGAAATGTC CACCACTGTT TCTCATCATC ACCAAACGAA
TGGCTGCAGT CTGTCCAGGC CACCATGATC CTGTCGATCA TCTTCAGCAT TCTGTCTCTG
TTCCTGTTCT TCTGCCAACT CTTCACCCTC ACCAAGGGGG GCAGGTTTTA CATCACTGGA
ATCTTCCAAA TTCTTGCTGG TCTGTGCGTG ATGAGTGCTG CGGCCATCTA CACGGTGAGG
CACCCGGAGT GGCATCTCAA CTCGGATTAC TCCTACGGTT TCGCCTACAT CCTGGCCTGG
GTGGCCTTCC CCCTGGCCCT TCTCAGCGGT GTCATCTATG TGATCTTGCG GAAACGCGAA
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16691
Accession Version NM_001281456.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 483bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product peripheral myelin protein 22
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB504971.1, BQ694106.1, CD519144.1, BQ212717.1, CK818720.1 and DV460945.1. Summary: This gene encodes an integral membrane protein that is a major component of myelin in the peripheral nervous system. Studies suggest two alternately used promoters drive tissue-specific expression. Various mutations of this gene are causes of Charcot-Marie-Tooth disease Type IA, Dejerine-Sottas syndrome, and hereditary neuropathy with liability to pressure palsies. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]. Transcript Variant: This variant (5) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3 ,4, and 5 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ694106.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962337, SAMEA962346 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
ATGCTCCTCC TGTTGCTGAG TATCATCGTC CTCCACGTCG CGGTGCTGGT GCTGCTGTTC 
GTCTCCACGA TCGTCAGCCA ATGGATCGTG GGCAATGGAC ACGCAACTGA TCTCTGGCAG
AACTGTAGCA CCTCTTCCTC AGGAAATGTC CACCACTGTT TCTCATCATC ACCAAACGAA
TGGCTGCAGT CTGTCCAGGC CACCATGATC CTGTCGATCA TCTTCAGCAT TCTGTCTCTG
TTCCTGTTCT TCTGCCAACT CTTCACCCTC ACCAAGGGGG GCAGGTTTTA CATCACTGGA
ATCTTCCAAA TTCTTGCTGG TCTGTGCGTG ATGAGTGCTG CGGCCATCTA CACGGTGAGG
CACCCGGAGT GGCATCTCAA CTCGGATTAC TCCTACGGTT TCGCCTACAT CCTGGCCTGG
GTGGCCTTCC CCCTGGCCCT TCTCAGCGGT GTCATCTATG TGATCTTGCG GAAACGCGAA
TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001268385.1
CDS235..717
Misc Feature(1)32..32(+)
Misc Feature(2)214..216(+)
Misc Feature(3)235..693(+)
Misc Feature(4)238..327(+)
Misc Feature(5)427..507(+)
Misc Feature(6)520..591(+)
Misc Feature(7)634..702(+)
Exon (1)1..204
Gene:PMP22
Gene Synonym:
Exon (2)205..312
Gene:PMP22
Gene Synonym:
Exon (3)313..412
Gene:PMP22
Gene Synonym:
Exon (4)413..553
Gene:PMP22
Gene Synonym:
Exon (5)554..1857
Gene:PMP22
Gene Synonym:
Translation
Position Chain Variation Link
16 16 c, t dbSNP:549380262
52 52 a, g dbSNP:143283160
62 62 c, t dbSNP:372385075
98 98 c, g dbSNP:560442424
105 105 a, g dbSNP:754758124
145 145 c, t dbSNP:184477058
153 153 a, c dbSNP:140650630
165 165 a, g dbSNP:114365663
192 192 a, g dbSNP:766242271
202 202 c, t dbSNP:543840509
208 208 a, c dbSNP:371965531
215 215 a, g dbSNP:533741268
217 217 g, t dbSNP:192086569
225 225 g, t dbSNP:773046723
229 229 g, t dbSNP:369779295
234 234 a, c dbSNP:748020473
252 252 c, g dbSNP:781550490
253 253 -, ag dbSNP:587776691
255 255 c, t dbSNP:755300278
259 259 a, g dbSNP:138515303
270 270 a, c dbSNP:104894622
281 281 c, t dbSNP:104894617
288 288 c, g dbSNP:780138909
299 299 c, t dbSNP:104894625
303 303 c, g dbSNP:376847762
306 306 a, c dbSNP:371373574
308 308 g, t dbSNP:765741053
316 316 c, t dbSNP:104894626
321 321 c, t dbSNP:201192820
322 322 a, c, g dbSNP:377335295
327 327 a, c dbSNP:779382797
329 329 a, g dbSNP:373322590
332 332 a, g dbSNP:754175558
333 333 a, g dbSNP:778072164
334 334 c, t dbSNP:756458019
336 336 a, c, g, t dbSNP:779654897
337 337 a, g dbSNP:750000952
338 338 c, g dbSNP:765235223
344 344 a, t dbSNP:104894627
354 354 g, t dbSNP:761801976
360 360 c, t dbSNP:776731388
362 362 c, g dbSNP:539999617
364 364 a, g, t dbSNP:112651887
386 386 a, g dbSNP:368908933
402 402 a, t dbSNP:771749546
406 406 c, t dbSNP:772242644
407 407 a, c dbSNP:745939923
410 410 a, g dbSNP:779004441
411 411 c, t dbSNP:376797385
418 418 c, t dbSNP:764071801
419 419 g, t dbSNP:756046682
422 422 a, t dbSNP:752712529
429 429 c, t dbSNP:767172855
433 433 a, c, g dbSNP:104894623
440 440 a, t dbSNP:104894620
444 444 c, t dbSNP:759343506
449 449 c, t dbSNP:104894621
450 450 a, g dbSNP:774493881
466 466 a, c dbSNP:766656618
468 468 a, g dbSNP:745546543
470 470 c, g dbSNP:104894618
472 472 -, ct dbSNP:786205087
472 472 c, g dbSNP:773201903
474 474 c, g dbSNP:769945527
475 475 c, t dbSNP:748551014
480 480 a, g dbSNP:779700333
489 489 c, g dbSNP:755701957
490 490 a, c dbSNP:11545341
500 500 a, c dbSNP:189205303
502 502 c, g dbSNP:147114400
509 509 a, g dbSNP:112232836
511 511 a, c, g dbSNP:778693173
512 512 c, g dbSNP:757021177
515 515 -, g dbSNP:80338763
515 515 -, g dbSNP:786205112
516 516 c, t dbSNP:750868826
526 526 a, c dbSNP:748778392
529 529 a, c dbSNP:777109239
542 542 a, g dbSNP:786204064
545 545 a, t dbSNP:755673309
550 550 a, g dbSNP:368223794
552 552 c, t dbSNP:778234032
558 558 a, g dbSNP:768210658
566 566 c, t dbSNP:759808649
572 572 c, t dbSNP:774831750
576 576 a, g dbSNP:201871607
578 578 -, ccatctacacgg dbSNP:786205111
578 578 c, t dbSNP:148822354
587 587 c, t dbSNP:104894619
588 588 g, t dbSNP:776275784
599 599 c, t dbSNP:768085434
600 600 a, g, t dbSNP:372723732
603 603 a, g dbSNP:558210039
604 604 a, c, t dbSNP:576618192
615 615 c, t dbSNP:369477577
617 617 c, t dbSNP:778731157
618 618 a, g dbSNP:757027432
630 630 c, t dbSNP:74361095
631 631 a, g dbSNP:140763467
636 636 c, t dbSNP:536417866
637 637 a, g dbSNP:141094419
641 641 a, c dbSNP:375449671
643 643 a, g dbSNP:755551524
645 645 c, t dbSNP:752025670
657 657 g, t dbSNP:144077495
660 660 c, t dbSNP:763227987
663 663 c, t dbSNP:776257683
664 664 a, c dbSNP:201255121
672 672 c, t dbSNP:533618640
681 681 c, t dbSNP:775019409
682 682 g, t dbSNP:104894624
692 692 a, g dbSNP:761138676
700 700 g, t dbSNP:771512763
702 702 a, g dbSNP:745772135
703 703 c, t dbSNP:28936682
705 705 a, g dbSNP:770693814
709 709 c, t dbSNP:749165928
710 710 a, g dbSNP:773478255
711 711 c, t dbSNP:778132801
716 716 a, g dbSNP:770193337
720 720 c, t dbSNP:373690370
721 721 a, g, t dbSNP:570955542
724 724 -, c dbSNP:758935587
725 725 a, t dbSNP:199650994
728 728 a, c, t dbSNP:772282020
729 729 a, g dbSNP:758748518
743 743 c, t dbSNP:200563670
749 749 c, t dbSNP:763703270
750 750 a, g dbSNP:201128796
754 754 a, g dbSNP:752369935
755 755 g, t dbSNP:549180136
776 776 a, c dbSNP:13422
792 792 a, g dbSNP:11545342
807 807 a, c dbSNP:774341981
809 809 a, c dbSNP:560368157
815 815 c, g dbSNP:541586082
819 819 a, g dbSNP:773184749
827 827 c, t dbSNP:769746708
832 832 -, caaac dbSNP:112829799
849 849 a, c, g dbSNP:781273381
853 853 a, g dbSNP:768773339
857 857 a, g dbSNP:747112334
861 861 c, t dbSNP:780587002
868 868 c, t dbSNP:572563714
874 874 a, g dbSNP:750957962
888 888 c, t dbSNP:779198796
890 890 c, t dbSNP:117277951
893 893 c, t dbSNP:752328330
915 915 c, g dbSNP:767142484
917 917 g, t dbSNP:142106420
922 922 a, c dbSNP:189734097
923 923 a, g dbSNP:766390986
926 926 a, t dbSNP:762946632
945 945 a, g dbSNP:1804193
952 952 a, g dbSNP:540148653
960 960 c, t dbSNP:533987307
1037 1037 -, t dbSNP:765955811
1073 1073 g, t dbSNP:373020223
1093 1093 c, t dbSNP:575964802
1136 1136 c, g dbSNP:533679946
1163 1163 c, g dbSNP:746771913
1243 1243 -, t dbSNP:16418
1244 1244 -, ct dbSNP:71699667
1259 1259 c, t dbSNP:184928176
1263 1263 c, t dbSNP:551380652
1267 1267 c, t dbSNP:775055426
1294 1294 c, t dbSNP:7538
1304 1304 c, t dbSNP:568822260
1352 1352 c, t dbSNP:557609991
1377 1377 -, aca dbSNP:371508374
1416 1416 c, t dbSNP:181257692
1481 1481 a, g dbSNP:747872532
1500 1500 a, t dbSNP:375638809
1535 1535 c, t dbSNP:529200588
1538 1538 c, g dbSNP:1803629
1545 1545 a, g dbSNP:13027
1556 1556 -, tta dbSNP:550359128
1585 1585 c, t dbSNP:754583973
1602 1602 c, t dbSNP:1804192
1607 1607 c, t dbSNP:546778557
1612 1612 a, g dbSNP:568790725
1629 1629 a, g dbSNP:149070440
1647 1647 c, t dbSNP:201970368
1648 1648 a, g dbSNP:552027045
1678 1678 c, g dbSNP:760879114
1705 1705 g, t dbSNP:545400789
1711 1711 a, c dbSNP:756594808
1744 1744 a, t dbSNP:189748556
1750 1750 c, g dbSNP:562876551
1766 1766 c, g dbSNP:750733887
1828 1828 g, t dbSNP:7415
1837 1837 c, t dbSNP:11654383

Target ORF information:

RefSeq Version NM_001281456
Organism Homo sapiens (human)
Definition Homo sapiens peripheral myelin protein 22 (PMP22), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001281456

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCTCCTCC TGTTGCTGAG TATCATCGTC CTCCACGTCG CGGTGCTGGT GCTGCTGTTC 
GTCTCCACGA TCGTCAGCCA ATGGATCGTG GGCAATGGAC ACGCAACTGA TCTCTGGCAG
AACTGTAGCA CCTCTTCCTC AGGAAATGTC CACCACTGTT TCTCATCATC ACCAAACGAA
TGGCTGCAGT CTGTCCAGGC CACCATGATC CTGTCGATCA TCTTCAGCAT TCTGTCTCTG
TTCCTGTTCT TCTGCCAACT CTTCACCCTC ACCAAGGGGG GCAGGTTTTA CATCACTGGA
ATCTTCCAAA TTCTTGCTGG TCTGTGCGTG ATGAGTGCTG CGGCCATCTA CACGGTGAGG
CACCCGGAGT GGCATCTCAA CTCGGATTAC TCCTACGGTT TCGCCTACAT CCTGGCCTGG
GTGGCCTTCC CCCTGGCCCT TCTCAGCGGT GTCATCTATG TGATCTTGCG GAAACGCGAA
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.