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POLE cDNA ORF clone, Homo sapiens (human)

Gene Symbol POLE
Entrez Gene ID 5426
Full Name polymerase (DNA directed), epsilon, catalytic subunit
Synonyms CRCS12, FILS, POLE1
General protein information
Preferred Names
DNA polymerase epsilon catalytic subunit A
Names
DNA polymerase epsilon catalytic subunit A
DNA polymerase II subunit A
NP_006222.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q24.3

Summary This gene encodes the catalytic subunit of DNA polymerase epsilon. The enzyme is involved in DNA repair and chromosomal DNA replication. Mutations in this gene have been associated with colorectal cancer 12 and facial dysmorphism, immunodeficiency, livedo, and short stature. [provided by RefSeq, Sep 2013]. lac of sum
Disorder MIM:

174762


mRNA and Protein(s)

mRNA Protein Name
XM_011534795 XP_011533097 DNA polymerase epsilon catalytic subunit A isoform X1
XM_011534796 XP_011533098 DNA polymerase epsilon catalytic subunit A isoform X2
XM_011534797 XP_011533099 DNA polymerase epsilon catalytic subunit A isoform X3
XM_011534798 XP_011533100 DNA polymerase epsilon catalytic subunit A isoform X4
XM_011534799 XP_011533101 DNA polymerase epsilon catalytic subunit A isoform X5
XM_011534800 XP_011533102 DNA polymerase epsilon catalytic subunit A isoform X6
XM_011534801 XP_011533103 DNA polymerase epsilon catalytic subunit A isoform X7
XM_011534802 XP_011533104 DNA polymerase epsilon catalytic subunit A isoform X8
NM_006231 NP_006222 DNA polymerase epsilon catalytic subunit A



Homo sapiens (human) POLE NP_006222.2
Pan troglodytes (chimpanzee) POLE XP_522564.4
Canis lupus familiaris (dog) POLE XP_543348.3
Bos taurus (cattle) POLE NP_001178358.1
Mus musculus (house mouse) Pole NP_035262.2
Rattus norvegicus (Norway rat) Pole NP_001100622.2
Gallus gallus (chicken) POLE XP_004934482.1
Danio rerio (zebrafish) pole NP_001121995.1
Drosophila melanogaster (fruit fly) DNApol-epsilon NP_524462.2
Caenorhabditis elegans F33H2.5 NP_493616.1
Arabidopsis thaliana (thale cress) TIL2 NP_180280.2
Arabidopsis thaliana (thale cress) TIL1 NP_172303.5
Xenopus (Silurana) tropicalis (western clawed frog) pole XP_004910692.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POLE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu59808 XM_011534795 PREDICTED: Homo sapiens polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1091.30
$1559.00
OHu59809 XM_011534796 PREDICTED: Homo sapiens polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1091.30
$1559.00
OHu59810 XM_011534797 PREDICTED: Homo sapiens polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $951.30
$1359.00
OHu59811 XM_011534798 PREDICTED: Homo sapiens polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $951.30
$1359.00
OHu59812 XM_011534799 PREDICTED: Homo sapiens polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $951.30
$1359.00
OHu59813 XM_011534800 PREDICTED: Homo sapiens polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $951.30
$1359.00
OHu59814 XM_011534801 PREDICTED: Homo sapiens polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu59815 XM_011534802 PREDICTED: Homo sapiens polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu20900 NM_006231 Homo sapiens polymerase (DNA directed), epsilon, catalytic subunit (POLE), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $923.30
$1319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu59808
Clone ID Related Accession (Same CDS sequence) XM_011534795
Accession Version XM_011534795.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6897bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA polymerase epsilon catalytic subunit A isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024477.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGA TCCTGAAGAG CATGGTCGTG
GGCTGGGTGA AGGAGATCAC CCAGTACCAC AACATCTATG CAGACAACCA GGTGATGCAC
TTCTACCGCT GGCTTCGGTC GCCATCCTCT CTGCTTCATG ACCCTGCCCT GCACCGCACA
CTCCACAACA TGATGAAGAA GCTCTTCCTG CAGCTCATCG CTGAGTTCAA GCGCCTGGGG
TCATCAGTCA TCTACGCCAA CTTCAACCGC ATCATCCTCT GTACAAAGAA GCGCCGTGTG
GAAGATGCCA TCGCTTACGT GGAGTACATC ACCAGCAGCA TCCATTCAAA GGAGACCTTC
CATTCTCTGA CAATTTCTTT CTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCATCT
AACTATGGCG GAATCAAAGG AAAAGTTTCA TCTCGTATTC ACTGTGGACT GCAAGACTCC
CAGAAAGCAG GGGGAGCAGA GGATGAGCAG GAAAATGAGG ACGATGAGGA GGAAAGAGAT
GGGGAGGAGG AGGAAGAGGC GGAGGAATCC AACGTGGAGG ATTTACTGGA AAACAACTGG
AACATTTTGC AGTTTTTGCC ACAGGCAGCC TCCTGCCAGA ACTACTTCCT CATGATTGTT
TCAGCGTACA TCGTGGCCGT GTACCACTGC ATGAAGGACG GGCTGAGGCG CAGTGCTCCA
GGGAGCACCC CCGTGAGGAG GAGGGGGGCC AGCCAGCTCT CCCAGGAGGC CGAGGGGGCG
GTCGGAGCCC TTCCCGGAAT GATCACCTTC TCTCAGGATT ATGTCGCAAA TGAGCTCACT
CAGAGCTTCT TCACCATCAC TCAGAAGATT CAGAAGAAAG TCACAGGCTC TCGGAACTCC
ACTGAGCTCT CAGAGATGTT TCCTGTCCTC CCCGGTTCCC ACTTGCTGCT CAATAACCCT
GCCCTGGAGT TCATCAAATA CGTGTGCAAG GTGCTGTCCC TGGACACCAA CATCACAAAC
CAGGTGAATA AGCTGAACCG AGACCTGCTT CGCCTGGTGG ATGTCGGCGA GTTCTCCGAG
GAGGCCCAGT TCCGAGACCC CTGCCGCTCC TACGTGCTTC CTGAGGTCAT CTGCCGCAGC
TGTAACTTCT GCCGCGACCT GGACCTGTGT AAAGACTCTT CCTTCTCAGA GGATGGGGCG
GTCCTGCCTC AGTGGCTCTG CTCCAACTGT CAGGCGCCCT ACGACTCCTC TGCCATCGAG
ATGACGCTGG TGGAAGTTCT ACAGAAGAAG CTGATGGCCT TCACCCTGCA GGACCTGCGG
AAGGAGGCAG ACACTCCCAA GTGTGAAGAG AAGGAAAGGA GGAAGAGGGA GTTGCACAGC
TCGGCCACAG GAAGGTCTTG CCGAGGGGCA TCTGAGCAGA GGCTGGGGGA CGCGCCAAGC
TGCCGTGTGG TGGTGCACGG CGTTCAGGCC TGCAGGATGG AGGGAGTGAG CAGGGCCTGG
AGGAGGCCTC AGGACTGGGC ATCTGGGGTG GAACATCCAC AGCGAGGGTG GGTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533097.1
CDS210..7106
Misc Feature(1)504..3704(+)
Misc Feature(2)1011..1622(+)
Misc Feature(3)1032..1595(+)
Misc Feature(4)1032..1595(+)
Misc Feature(5)1035..1595(+)
Misc Feature(6)1800..3668(+)
Misc Feature(7)2085..2795(+)
Misc Feature(8)2787..2798(+)
Misc Feature(9)4782..5984(+)
Translation

Target ORF information:

RefSeq Version XM_011534795
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011534795

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGA TCCTGAAGAG CATGGTCGTG
GGCTGGGTGA AGGAGATCAC CCAGTACCAC AACATCTATG CAGACAACCA GGTGATGCAC
TTCTACCGCT GGCTTCGGTC GCCATCCTCT CTGCTTCATG ACCCTGCCCT GCACCGCACA
CTCCACAACA TGATGAAGAA GCTCTTCCTG CAGCTCATCG CTGAGTTCAA GCGCCTGGGG
TCATCAGTCA TCTACGCCAA CTTCAACCGC ATCATCCTCT GTACAAAGAA GCGCCGTGTG
GAAGATGCCA TCGCTTACGT GGAGTACATC ACCAGCAGCA TCCATTCAAA GGAGACCTTC
CATTCTCTGA CAATTTCTTT CTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCATCT
AACTATGGCG GAATCAAAGG AAAAGTTTCA TCTCGTATTC ACTGTGGACT GCAAGACTCC
CAGAAAGCAG GGGGAGCAGA GGATGAGCAG GAAAATGAGG ACGATGAGGA GGAAAGAGAT
GGGGAGGAGG AGGAAGAGGC GGAGGAATCC AACGTGGAGG ATTTACTGGA AAACAACTGG
AACATTTTGC AGTTTTTGCC ACAGGCAGCC TCCTGCCAGA ACTACTTCCT CATGATTGTT
TCAGCGTACA TCGTGGCCGT GTACCACTGC ATGAAGGACG GGCTGAGGCG CAGTGCTCCA
GGGAGCACCC CCGTGAGGAG GAGGGGGGCC AGCCAGCTCT CCCAGGAGGC CGAGGGGGCG
GTCGGAGCCC TTCCCGGAAT GATCACCTTC TCTCAGGATT ATGTCGCAAA TGAGCTCACT
CAGAGCTTCT TCACCATCAC TCAGAAGATT CAGAAGAAAG TCACAGGCTC TCGGAACTCC
ACTGAGCTCT CAGAGATGTT TCCTGTCCTC CCCGGTTCCC ACTTGCTGCT CAATAACCCT
GCCCTGGAGT TCATCAAATA CGTGTGCAAG GTGCTGTCCC TGGACACCAA CATCACAAAC
CAGGTGAATA AGCTGAACCG AGACCTGCTT CGCCTGGTGG ATGTCGGCGA GTTCTCCGAG
GAGGCCCAGT TCCGAGACCC CTGCCGCTCC TACGTGCTTC CTGAGGTCAT CTGCCGCAGC
TGTAACTTCT GCCGCGACCT GGACCTGTGT AAAGACTCTT CCTTCTCAGA GGATGGGGCG
GTCCTGCCTC AGTGGCTCTG CTCCAACTGT CAGGCGCCCT ACGACTCCTC TGCCATCGAG
ATGACGCTGG TGGAAGTTCT ACAGAAGAAG CTGATGGCCT TCACCCTGCA GGACCTGCGG
AAGGAGGCAG ACACTCCCAA GTGTGAAGAG AAGGAAAGGA GGAAGAGGGA GTTGCACAGC
TCGGCCACAG GAAGGTCTTG CCGAGGGGCA TCTGAGCAGA GGCTGGGGGA CGCGCCAAGC
TGCCGTGTGG TGGTGCACGG CGTTCAGGCC TGCAGGATGG AGGGAGTGAG CAGGGCCTGG
AGGAGGCCTC AGGACTGGGC ATCTGGGGTG GAACATCCAC AGCGAGGGTG GGTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59809
Clone ID Related Accession (Same CDS sequence) XM_011534796
Accession Version XM_011534796.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6768bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA polymerase epsilon catalytic subunit A isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024477.15) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
ATGGATTTGC GGTTTGGTTT TGAGCGGCTG AAGGAGCCTG GTGAGAAGAC AGGCTGGCTC 
ATTAACATGC ATCCTACCGA GATTTTAGAT GAAGATAAGC GCTTAGGCAG TGCAGTGGAT
TACTACTTTA TTCAAGATGA CGGAAGCAGA TTTAAGGTGG CTTTGCCCTA TAAACCGTAT
TTCTACATTG CGACCAGAAA GGGTTGTGAG CGAGAAGTTT CATCTTTTCT CTCCAAGAAG
TTTCAGGGCA AAATTGCAAA AGTGGAGACT GTCCCCAAAG AGGATCTGGA CTTGCCAAAT
CACTTGGTGG GTTTGAAGCG AAATTACATC AGGCTGTCCT TCCACACTGT GGAGGATCTT
GTCAAAGTGA GGAAGGAGAT CTCCCCTGCC GTGAAGAAGA ACAGGGAGCA GGATCACGCC
AGCGACGCGT ACACAGCTCT GCTTTCCAGT GTTCTGCAGA GGGGCGGTGT CATTACTGAT
GAAGAGGAAA CCTCTAAGAA GATAGCTGAC CAGTTGGACA ACATTGTGGA CATGCGCGAG
TACGATGTTC CCTACCACAT CCGCCTCTCC ATTGACCTGA AGATCCACGT GGCTCATTGG
TACAATGTCA GATACCGAGG AAATGCTTTT CCGGTAGAAA TCACCCGCCG AGATGACCTT
GTTGAACGAC CTGACCCTGT GGTTTTGGCA TTTGACATTG AGACGACCAA ACTGCCCCTC
AAGTTTCCTG ATGCTGAGAC AGACCAGATT ATGATGATTT CCTACATGAT CGATGGCCAG
GGCTACCTCA TCACCAACAG GGAGATTGTT TCAGAAGATA TTGAAGATTT TGAGTTCACC
CCCAAGCCAG AATATGAAGG CCCCTTTTGT GTCTTCAATG AACCCGATGA GGCTCATCTG
ATCCAAAGGT GGTTTGAACA CGTCCAGGAG ACCAAACCCA CCATCATGGT CACCTACAAC
GGGGACTTTT TTGACTGGCC ATTTGTGGAG GCCCGGGCAG CAGTCCACGG TCTGAGCATG
CAGCAGGAGA TAGGCTTCCA GAAGGACAGC CAGGGGGAGT ACAAGGCGCC CCAGTGCATC
CACATGGACT GCCTCAGGTG GGTGAAGAGG GACAGTTACC TTCCTGTGGG CAGTCATAAT
CTCAAGGCGG CCGCCAAGGC CAAGCTAGGC TATGATCCCG TGGAGCTAGA CCCGGAGGAC
ATGTGCCGGA TGGCCACGGA GCAGCCCCAG ACTCTGGCCA CGTATTCTGT GTCAGATGCT
GTCGCCACTT ACTACCTGTA CATGAAGTAC GTCCACCCAT TCATCTTTGC TCTGTGCACC
ATTATTCCCA TGGAGCCCGA CGAGGTGCTG CGGAAGGGCT CTGGCACTCT GTGTGAGGCC
TTGCTGATGG TGCAGGCCTT CCACGCCAAC ATCATCTTCC CCAACAAGCA AGAGCAGGAG
TTCAATAAGC TGACGGACGA CGGACACGTG CTGGACTCTG AGACCTACGT CGGGGGCCAC
GTGGAGGCCC TCGAGTCTGG GGTTTTCCGC AGCGATATCC CTTGCCGGTT TAGGATGAAT
CCTGCCGCCT TTGACTTCCT GCTGCAGCGG GTTGAGAAGA CCTTGCGCCA CGCCCTTGAG
GAAGAGGAGA AAGTGCCTGT GGAGCAAGTC ACCAACTTTG AAGAGGTGTG TGATGAGATT
AAGAGCAAGC TTGCCTCCCT GAAGGACGTT CCCAGCCGCA TCGAGTGTCC ACTCATCTAC
CACCTGGACG TGGGGGCCAT GTACCCCAAC ATCATCCTGA CCAACCGCCT GCAGCCCTCT
GCCATGGTGG ACGAAGCCAC CTGTGCTGCC TGTGACTTCA ATAAGCCTGG AGCAAACTGC
CAGCGGAAGA TGGCCTGGCA GTGGAGGGGC GAGTTCATGC CAGCCAGTCG CAGCGAATAC
CATCGGATCC AGCACCAGCT GGAGTCAGAG AAGTTCCCCC CCTTGTTCCC AGAGGGGCCA
GCTCGGGCCT TTCATGAACT GTCCCGCGAG GAACAGGCGA AATACGAGAA GAGAAGGCTG
GCGGATTACT GCCGGAAAGC CTACAAGAAG ATCCACATCA CCAAGGTGGA AGAGCGTCTC
ACCACCATCT GCCAGCGGGA AAACTCCTTC TACGTGGACA CCGTGCGTGC CTTCCGGGAC
AGGCGTTACG AGTTCAAAGG GCTCCACAAG GTGTGGAAAA AGAAGCTCTC GGCGGCCGTG
GAGGTGGGCG ACGCGGCTGA GGTGAAGCGC TGCAAGAACA TGGAGGTGCT GTATGACTCG
CTGCAGCTGG CCCACAAGTG CATCCTGAAC TCCTTCTATG GCTATGTCAT GCGCAAGGGG
GCTCGCTGGT ACTCCATGGA GATGGCTGGC ATCGTCTGCT TCACAGGGGC CAACATCATC
ACCCAGGCAC GGGAGCTGAT CGAGCAGATT GGGAGGCCCT TAGAGCTGGA CACAGATGGT
ATATGGTGCG TCCTGCCCAA CAGCTTCCCA GAAAATTTTG TCTTCAAGAC GACCAATGTG
AAGAAGCCCA AAGTGACCAT CTCCTACCCA GGCGCCATGT TGAACATCAT GGTCAAGGAA
GGCTTCACCA ATGACCAGTA CCAGGAGCTG GCTGAGCCGT CCTCACTCAC CTACGTCACC
CGCTCAGAGA ACAGCATCTT TTTTGAGGTT GATGGGCCCT ACCTTGCCAT GATTCTTCCA
GCCTCCAAGG AAGAAGGCAA GAAATTGAAG AAGAGGTATG CTGTGTTCAA TGAAGACGGT
TCTCTGGCTG AGCTCAAGGG CTTTGAGGTC AAACGCCGCG GGGAACTGCA GCTGATTAAG
ATCTTCCAAT CCTCGGTGTT TGAGGCCTTC CTCAAGGGCA GCACGCTGGA AGAGGTGTAT
GGCTCTGTAG CCAAGGTGGC TGACTACTGG CTGGACGTGC TGTACAGCAA GGCAGCCAAC
ATGCCTGACT CTGAGCTATT CGAGCTCATC TCTGAGAACC GTTCCATGTC TCGGAAGCTG
GAAGATTACG GGGAGCAGAA GTCTACGTCC ATCAGCACAG CAAAGCGCCT GGCCGAGTTC
CTGGGAGACC AGATGGTCAA GGATGCAGGG CTGAGTTGCC GCTACATCAT CTCCCGCAAG
CCCGAGGGCT CCCCTGTCAC GGAGAGGGCC ATCCCACTTG CCATTTTCCA AGCAGAGCCC
ACGGTGAGGA AGCACTTTCT CCGGAAATGG CTCAAGAGCT CTTCCCTTCA AGACTTTGAT
ATTCGAGCAA TTCTGGATTG GGACTACTAC ATTGAGCGGC TGGGAAGCGC CATCCAGAAG
ATCATCACCA TCCCTGCGGC CCTGCAGCAG GTAAAGAACC CAGTGCCACG TGTCAAACAC
CCCGACTGGC TGCACAAAAA ACTGCTGGAG AAGAATGATG TCTACAAGCA GAAGAAGATC
AGTGAGCTCT TCACCCTGGA GGGCAGGAGA CAGGTCACGA TGGCCGAGGC CTCAGAAGAC
AGTCCGAGGC CAAGTGCTCC TGACATGGAG GACTTCGGCC TCGTAAAGCT GCCTCACCCA
GCAGCCCCTG TCACTGTGAA GAGGAAGCGA GTTCTTTGGG AGAGCCAGGA GGAGTCCCAG
GACCTCACGC CGACTGTGCC CTGGCAGGAA ATCTTGGGGC AGCCTCCCGC CCTGGGAACC
AGCCAGGAGG AATGGCTTGT CTGGCTCCGG TTCCACAAGA AGAAGTGGCA GCTGCAGGCC
CGGCAGCGCC TCGCCCGCAG GAAGAGGCAG CGTCTGGAGT CGGCAGAGGG TGTGCTCAGG
CCCGGGGCCA TCCGGGATGG TCCTGCCACG GGGCTGGGGA GCTTCTTGCG AAGAACTGCC
CGCAGCATCC TGGACCTTCC GTGGCAGATT GTGCAGATCA GCGAGACCAG CCAGGCCGGC
CTGTTCAGGC TGTGGGCGCT CGTTGGCAGT GACTTGCACT GCATCAGGCT GAGCATCCCC
CGTGTGTTCT ACGTGAACCA GCGAGTCGCT AAAGCGGAGG AGGGTGCTTC GTATCGCAAG
GTAAATCGGG TCCTTCCTCG CTCCAACATG GTCTACAATC TCTATGAGTA TTCAGTGCCA
GAGGACATGT ACCAGGAACA CATCAACGAG ATCAACGCTG AGCTGTCAGC GCCAGACATC
GAGGGCGTAT ATGAGACTCA GGTTCCGTTA CTGTTCCGGG CCCTGGTGCA CCTGGGCTGT
GTGTGTGTGG TCAATAAACA GCTGGTGAGG CACCTTTCAG GCTGGGAAGC AGAGACCTTT
GCTCTTGAGC ACCTGGAGAT GCGCTCTCTG GCCCAGTTCA GCTACCTGGA ACCAGGGAGT
ATCCGCCATA TCTACCTGTA CCACCACGCA CAGGCCCACA AAGCGCTCTT CGGGATCTTC
ATCCCCTCAC AGCGCAGGGC ATCCGTCTTT GTGCTGGACA CTGTGCGCAG CAACCAGATG
CCCAGCCTTG GCGCCCTGTA CTCAGCAGAG CACGGCCTCC TCCTGGAGAA GGTGGGCCCT
GAGCTCCTGC CACCCCCCAA ACACACCTTC GAAGTTCGGG CAGAAACTGA CCTGAAGACC
ATCTGCAGAG CCATCCAGCG ATTCCTGCTC GCCTACAAGG AGGAGCGCCG GGGGCCCACA
CTCATCGCTG TTCAGTCCAG CTGGGAGCTG AAGAGGCTGG CCAGTGAAAT TCCTGTCTTG
GAGGAATTCC CACTGGTGCC TATCTGTGTG GCTGACAAGA TCAACTATGG GGTCCTGGAC
TGGCAGCGCC ATGGAGCCCG GCGCATGATC CGTCACTACC TCAACCTGGA CACCTGCCTG
TCGCAGGCCT TCGAGATGAG CAGGTACTTT CACATTCCCA TTGGGAACCT ACCAGAGGAC
ATCTCCACAT TCGGCTCCGA CCTCTTCTTT GCCCGCCACC TCCAGCGCCA CAACCACCTG
CTCTGGCTGT CCCCTACAGC CCGCCCTGAC CTGGGTGGAA AGGAGGCTGA TGACAACTGT
CTTGTCATGG AGTTCGATGA CCAAGCCACT GTTGAGATCA ACAGTTCAGG CTGTTACTCC
ACAGTGTGTG TGGAGCTGGA CCTTCAGAAC CTGGCCGTCA ACACCATTCT CCAGTCTCAC
CATGTCAACG ACATGGAGGG GGCCGACAGC ATGGGGATCA GCTTCGACGT GATCCAGCAG
GCCTCCCTGG AGGACATGAT CACGGGTGGT CAGGCTGCCA GTGCCCCGGC CAGCTACGAT
GAGACAGCCC TGTGCTCTAA CACCTTCAGG ATCCTGAAGA GCATGGTCGT GGGCTGGGTG
AAGGAGATCA CCCAGTACCA CAACATCTAT GCAGACAACC AGGTGATGCA CTTCTACCGC
TGGCTTCGGT CGCCATCCTC TCTGCTTCAT GACCCTGCCC TGCACCGCAC ACTCCACAAC
ATGATGAAGA AGCTCTTCCT GCAGCTCATC GCTGAGTTCA AGCGCCTGGG GTCATCAGTC
ATCTACGCCA ACTTCAACCG CATCATCCTC TGTACAAAGA AGCGCCGTGT GGAAGATGCC
ATCGCTTACG TGGAGTACAT CACCAGCAGC ATCCATTCAA AGGAGACCTT CCATTCTCTG
ACAATTTCTT TCTCTCGATG CTGGGAATTT CTTCTCTGGA TGGATCCATC TAACTATGGC
GGAATCAAAG GAAAAGTTTC ATCTCGTATT CACTGTGGAC TGCAAGACTC CCAGAAAGCA
GGGGGAGCAG AGGATGAGCA GGAAAATGAG GACGATGAGG AGGAAAGAGA TGGGGAGGAG
GAGGAAGAGG CGGAGGAATC CAACGTGGAG GATTTACTGG AAAACAACTG GAACATTTTG
CAGTTTTTGC CACAGGCAGC CTCCTGCCAG AACTACTTCC TCATGATTGT TTCAGCGTAC
ATCGTGGCCG TGTACCACTG CATGAAGGAC GGGCTGAGGC GCAGTGCTCC AGGGAGCACC
CCCGTGAGGA GGAGGGGGGC CAGCCAGCTC TCCCAGGAGG CCGAGGGGGC GGTCGGAGCC
CTTCCCGGAA TGATCACCTT CTCTCAGGAT TATGTCGCAA ATGAGCTCAC TCAGAGCTTC
TTCACCATCA CTCAGAAGAT TCAGAAGAAA GTCACAGGCT CTCGGAACTC CACTGAGCTC
TCAGAGATGT TTCCTGTCCT CCCCGGTTCC CACTTGCTGC TCAATAACCC TGCCCTGGAG
TTCATCAAAT ACGTGTGCAA GGTGCTGTCC CTGGACACCA ACATCACAAA CCAGGTGAAT
AAGCTGAACC GAGACCTGCT TCGCCTGGTG GATGTCGGCG AGTTCTCCGA GGAGGCCCAG
TTCCGAGACC CCTGCCGCTC CTACGTGCTT CCTGAGGTCA TCTGCCGCAG CTGTAACTTC
TGCCGCGACC TGGACCTGTG TAAAGACTCT TCCTTCTCAG AGGATGGGGC GGTCCTGCCT
CAGTGGCTCT GCTCCAACTG TCAGGCGCCC TACGACTCCT CTGCCATCGA GATGACGCTG
GTGGAAGTTC TACAGAAGAA GCTGATGGCC TTCACCCTGC AGGACCTGCG GAAGGAGGCA
GACACTCCCA AGTGTGAAGA GAAGGAAAGG AGGAAGAGGG AGTTGCACAG CTCGGCCACA
GGAAGGTCTT GCCGAGGGGC ATCTGAGCAG AGGCTGGGGG ACGCGCCAAG CTGCCGTGTG
GTGGTGCACG GCGTTCAGGC CTGCAGGATG GAGGGAGTGA GCAGGGCCTG GAGGAGGCCT
CAGGACTGGG CATCTGGGGT GGAACATCCA CAGCGAGGGT GGGTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533098.1
CDS135..6902
Misc Feature(1)300..3500(+)
Misc Feature(2)807..1418(+)
Misc Feature(3)828..1391(+)
Misc Feature(4)828..1391(+)
Misc Feature(5)831..1391(+)
Misc Feature(6)1596..3464(+)
Misc Feature(7)1881..2591(+)
Misc Feature(8)2583..2594(+)
Misc Feature(9)4578..5780(+)
Translation

Target ORF information:

RefSeq Version XM_011534796
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011534796

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
ATGGATTTGC GGTTTGGTTT TGAGCGGCTG AAGGAGCCTG GTGAGAAGAC AGGCTGGCTC 
ATTAACATGC ATCCTACCGA GATTTTAGAT GAAGATAAGC GCTTAGGCAG TGCAGTGGAT
TACTACTTTA TTCAAGATGA CGGAAGCAGA TTTAAGGTGG CTTTGCCCTA TAAACCGTAT
TTCTACATTG CGACCAGAAA GGGTTGTGAG CGAGAAGTTT CATCTTTTCT CTCCAAGAAG
TTTCAGGGCA AAATTGCAAA AGTGGAGACT GTCCCCAAAG AGGATCTGGA CTTGCCAAAT
CACTTGGTGG GTTTGAAGCG AAATTACATC AGGCTGTCCT TCCACACTGT GGAGGATCTT
GTCAAAGTGA GGAAGGAGAT CTCCCCTGCC GTGAAGAAGA ACAGGGAGCA GGATCACGCC
AGCGACGCGT ACACAGCTCT GCTTTCCAGT GTTCTGCAGA GGGGCGGTGT CATTACTGAT
GAAGAGGAAA CCTCTAAGAA GATAGCTGAC CAGTTGGACA ACATTGTGGA CATGCGCGAG
TACGATGTTC CCTACCACAT CCGCCTCTCC ATTGACCTGA AGATCCACGT GGCTCATTGG
TACAATGTCA GATACCGAGG AAATGCTTTT CCGGTAGAAA TCACCCGCCG AGATGACCTT
GTTGAACGAC CTGACCCTGT GGTTTTGGCA TTTGACATTG AGACGACCAA ACTGCCCCTC
AAGTTTCCTG ATGCTGAGAC AGACCAGATT ATGATGATTT CCTACATGAT CGATGGCCAG
GGCTACCTCA TCACCAACAG GGAGATTGTT TCAGAAGATA TTGAAGATTT TGAGTTCACC
CCCAAGCCAG AATATGAAGG CCCCTTTTGT GTCTTCAATG AACCCGATGA GGCTCATCTG
ATCCAAAGGT GGTTTGAACA CGTCCAGGAG ACCAAACCCA CCATCATGGT CACCTACAAC
GGGGACTTTT TTGACTGGCC ATTTGTGGAG GCCCGGGCAG CAGTCCACGG TCTGAGCATG
CAGCAGGAGA TAGGCTTCCA GAAGGACAGC CAGGGGGAGT ACAAGGCGCC CCAGTGCATC
CACATGGACT GCCTCAGGTG GGTGAAGAGG GACAGTTACC TTCCTGTGGG CAGTCATAAT
CTCAAGGCGG CCGCCAAGGC CAAGCTAGGC TATGATCCCG TGGAGCTAGA CCCGGAGGAC
ATGTGCCGGA TGGCCACGGA GCAGCCCCAG ACTCTGGCCA CGTATTCTGT GTCAGATGCT
GTCGCCACTT ACTACCTGTA CATGAAGTAC GTCCACCCAT TCATCTTTGC TCTGTGCACC
ATTATTCCCA TGGAGCCCGA CGAGGTGCTG CGGAAGGGCT CTGGCACTCT GTGTGAGGCC
TTGCTGATGG TGCAGGCCTT CCACGCCAAC ATCATCTTCC CCAACAAGCA AGAGCAGGAG
TTCAATAAGC TGACGGACGA CGGACACGTG CTGGACTCTG AGACCTACGT CGGGGGCCAC
GTGGAGGCCC TCGAGTCTGG GGTTTTCCGC AGCGATATCC CTTGCCGGTT TAGGATGAAT
CCTGCCGCCT TTGACTTCCT GCTGCAGCGG GTTGAGAAGA CCTTGCGCCA CGCCCTTGAG
GAAGAGGAGA AAGTGCCTGT GGAGCAAGTC ACCAACTTTG AAGAGGTGTG TGATGAGATT
AAGAGCAAGC TTGCCTCCCT GAAGGACGTT CCCAGCCGCA TCGAGTGTCC ACTCATCTAC
CACCTGGACG TGGGGGCCAT GTACCCCAAC ATCATCCTGA CCAACCGCCT GCAGCCCTCT
GCCATGGTGG ACGAAGCCAC CTGTGCTGCC TGTGACTTCA ATAAGCCTGG AGCAAACTGC
CAGCGGAAGA TGGCCTGGCA GTGGAGGGGC GAGTTCATGC CAGCCAGTCG CAGCGAATAC
CATCGGATCC AGCACCAGCT GGAGTCAGAG AAGTTCCCCC CCTTGTTCCC AGAGGGGCCA
GCTCGGGCCT TTCATGAACT GTCCCGCGAG GAACAGGCGA AATACGAGAA GAGAAGGCTG
GCGGATTACT GCCGGAAAGC CTACAAGAAG ATCCACATCA CCAAGGTGGA AGAGCGTCTC
ACCACCATCT GCCAGCGGGA AAACTCCTTC TACGTGGACA CCGTGCGTGC CTTCCGGGAC
AGGCGTTACG AGTTCAAAGG GCTCCACAAG GTGTGGAAAA AGAAGCTCTC GGCGGCCGTG
GAGGTGGGCG ACGCGGCTGA GGTGAAGCGC TGCAAGAACA TGGAGGTGCT GTATGACTCG
CTGCAGCTGG CCCACAAGTG CATCCTGAAC TCCTTCTATG GCTATGTCAT GCGCAAGGGG
GCTCGCTGGT ACTCCATGGA GATGGCTGGC ATCGTCTGCT TCACAGGGGC CAACATCATC
ACCCAGGCAC GGGAGCTGAT CGAGCAGATT GGGAGGCCCT TAGAGCTGGA CACAGATGGT
ATATGGTGCG TCCTGCCCAA CAGCTTCCCA GAAAATTTTG TCTTCAAGAC GACCAATGTG
AAGAAGCCCA AAGTGACCAT CTCCTACCCA GGCGCCATGT TGAACATCAT GGTCAAGGAA
GGCTTCACCA ATGACCAGTA CCAGGAGCTG GCTGAGCCGT CCTCACTCAC CTACGTCACC
CGCTCAGAGA ACAGCATCTT TTTTGAGGTT GATGGGCCCT ACCTTGCCAT GATTCTTCCA
GCCTCCAAGG AAGAAGGCAA GAAATTGAAG AAGAGGTATG CTGTGTTCAA TGAAGACGGT
TCTCTGGCTG AGCTCAAGGG CTTTGAGGTC AAACGCCGCG GGGAACTGCA GCTGATTAAG
ATCTTCCAAT CCTCGGTGTT TGAGGCCTTC CTCAAGGGCA GCACGCTGGA AGAGGTGTAT
GGCTCTGTAG CCAAGGTGGC TGACTACTGG CTGGACGTGC TGTACAGCAA GGCAGCCAAC
ATGCCTGACT CTGAGCTATT CGAGCTCATC TCTGAGAACC GTTCCATGTC TCGGAAGCTG
GAAGATTACG GGGAGCAGAA GTCTACGTCC ATCAGCACAG CAAAGCGCCT GGCCGAGTTC
CTGGGAGACC AGATGGTCAA GGATGCAGGG CTGAGTTGCC GCTACATCAT CTCCCGCAAG
CCCGAGGGCT CCCCTGTCAC GGAGAGGGCC ATCCCACTTG CCATTTTCCA AGCAGAGCCC
ACGGTGAGGA AGCACTTTCT CCGGAAATGG CTCAAGAGCT CTTCCCTTCA AGACTTTGAT
ATTCGAGCAA TTCTGGATTG GGACTACTAC ATTGAGCGGC TGGGAAGCGC CATCCAGAAG
ATCATCACCA TCCCTGCGGC CCTGCAGCAG GTAAAGAACC CAGTGCCACG TGTCAAACAC
CCCGACTGGC TGCACAAAAA ACTGCTGGAG AAGAATGATG TCTACAAGCA GAAGAAGATC
AGTGAGCTCT TCACCCTGGA GGGCAGGAGA CAGGTCACGA TGGCCGAGGC CTCAGAAGAC
AGTCCGAGGC CAAGTGCTCC TGACATGGAG GACTTCGGCC TCGTAAAGCT GCCTCACCCA
GCAGCCCCTG TCACTGTGAA GAGGAAGCGA GTTCTTTGGG AGAGCCAGGA GGAGTCCCAG
GACCTCACGC CGACTGTGCC CTGGCAGGAA ATCTTGGGGC AGCCTCCCGC CCTGGGAACC
AGCCAGGAGG AATGGCTTGT CTGGCTCCGG TTCCACAAGA AGAAGTGGCA GCTGCAGGCC
CGGCAGCGCC TCGCCCGCAG GAAGAGGCAG CGTCTGGAGT CGGCAGAGGG TGTGCTCAGG
CCCGGGGCCA TCCGGGATGG TCCTGCCACG GGGCTGGGGA GCTTCTTGCG AAGAACTGCC
CGCAGCATCC TGGACCTTCC GTGGCAGATT GTGCAGATCA GCGAGACCAG CCAGGCCGGC
CTGTTCAGGC TGTGGGCGCT CGTTGGCAGT GACTTGCACT GCATCAGGCT GAGCATCCCC
CGTGTGTTCT ACGTGAACCA GCGAGTCGCT AAAGCGGAGG AGGGTGCTTC GTATCGCAAG
GTAAATCGGG TCCTTCCTCG CTCCAACATG GTCTACAATC TCTATGAGTA TTCAGTGCCA
GAGGACATGT ACCAGGAACA CATCAACGAG ATCAACGCTG AGCTGTCAGC GCCAGACATC
GAGGGCGTAT ATGAGACTCA GGTTCCGTTA CTGTTCCGGG CCCTGGTGCA CCTGGGCTGT
GTGTGTGTGG TCAATAAACA GCTGGTGAGG CACCTTTCAG GCTGGGAAGC AGAGACCTTT
GCTCTTGAGC ACCTGGAGAT GCGCTCTCTG GCCCAGTTCA GCTACCTGGA ACCAGGGAGT
ATCCGCCATA TCTACCTGTA CCACCACGCA CAGGCCCACA AAGCGCTCTT CGGGATCTTC
ATCCCCTCAC AGCGCAGGGC ATCCGTCTTT GTGCTGGACA CTGTGCGCAG CAACCAGATG
CCCAGCCTTG GCGCCCTGTA CTCAGCAGAG CACGGCCTCC TCCTGGAGAA GGTGGGCCCT
GAGCTCCTGC CACCCCCCAA ACACACCTTC GAAGTTCGGG CAGAAACTGA CCTGAAGACC
ATCTGCAGAG CCATCCAGCG ATTCCTGCTC GCCTACAAGG AGGAGCGCCG GGGGCCCACA
CTCATCGCTG TTCAGTCCAG CTGGGAGCTG AAGAGGCTGG CCAGTGAAAT TCCTGTCTTG
GAGGAATTCC CACTGGTGCC TATCTGTGTG GCTGACAAGA TCAACTATGG GGTCCTGGAC
TGGCAGCGCC ATGGAGCCCG GCGCATGATC CGTCACTACC TCAACCTGGA CACCTGCCTG
TCGCAGGCCT TCGAGATGAG CAGGTACTTT CACATTCCCA TTGGGAACCT ACCAGAGGAC
ATCTCCACAT TCGGCTCCGA CCTCTTCTTT GCCCGCCACC TCCAGCGCCA CAACCACCTG
CTCTGGCTGT CCCCTACAGC CCGCCCTGAC CTGGGTGGAA AGGAGGCTGA TGACAACTGT
CTTGTCATGG AGTTCGATGA CCAAGCCACT GTTGAGATCA ACAGTTCAGG CTGTTACTCC
ACAGTGTGTG TGGAGCTGGA CCTTCAGAAC CTGGCCGTCA ACACCATTCT CCAGTCTCAC
CATGTCAACG ACATGGAGGG GGCCGACAGC ATGGGGATCA GCTTCGACGT GATCCAGCAG
GCCTCCCTGG AGGACATGAT CACGGGTGGT CAGGCTGCCA GTGCCCCGGC CAGCTACGAT
GAGACAGCCC TGTGCTCTAA CACCTTCAGG ATCCTGAAGA GCATGGTCGT GGGCTGGGTG
AAGGAGATCA CCCAGTACCA CAACATCTAT GCAGACAACC AGGTGATGCA CTTCTACCGC
TGGCTTCGGT CGCCATCCTC TCTGCTTCAT GACCCTGCCC TGCACCGCAC ACTCCACAAC
ATGATGAAGA AGCTCTTCCT GCAGCTCATC GCTGAGTTCA AGCGCCTGGG GTCATCAGTC
ATCTACGCCA ACTTCAACCG CATCATCCTC TGTACAAAGA AGCGCCGTGT GGAAGATGCC
ATCGCTTACG TGGAGTACAT CACCAGCAGC ATCCATTCAA AGGAGACCTT CCATTCTCTG
ACAATTTCTT TCTCTCGATG CTGGGAATTT CTTCTCTGGA TGGATCCATC TAACTATGGC
GGAATCAAAG GAAAAGTTTC ATCTCGTATT CACTGTGGAC TGCAAGACTC CCAGAAAGCA
GGGGGAGCAG AGGATGAGCA GGAAAATGAG GACGATGAGG AGGAAAGAGA TGGGGAGGAG
GAGGAAGAGG CGGAGGAATC CAACGTGGAG GATTTACTGG AAAACAACTG GAACATTTTG
CAGTTTTTGC CACAGGCAGC CTCCTGCCAG AACTACTTCC TCATGATTGT TTCAGCGTAC
ATCGTGGCCG TGTACCACTG CATGAAGGAC GGGCTGAGGC GCAGTGCTCC AGGGAGCACC
CCCGTGAGGA GGAGGGGGGC CAGCCAGCTC TCCCAGGAGG CCGAGGGGGC GGTCGGAGCC
CTTCCCGGAA TGATCACCTT CTCTCAGGAT TATGTCGCAA ATGAGCTCAC TCAGAGCTTC
TTCACCATCA CTCAGAAGAT TCAGAAGAAA GTCACAGGCT CTCGGAACTC CACTGAGCTC
TCAGAGATGT TTCCTGTCCT CCCCGGTTCC CACTTGCTGC TCAATAACCC TGCCCTGGAG
TTCATCAAAT ACGTGTGCAA GGTGCTGTCC CTGGACACCA ACATCACAAA CCAGGTGAAT
AAGCTGAACC GAGACCTGCT TCGCCTGGTG GATGTCGGCG AGTTCTCCGA GGAGGCCCAG
TTCCGAGACC CCTGCCGCTC CTACGTGCTT CCTGAGGTCA TCTGCCGCAG CTGTAACTTC
TGCCGCGACC TGGACCTGTG TAAAGACTCT TCCTTCTCAG AGGATGGGGC GGTCCTGCCT
CAGTGGCTCT GCTCCAACTG TCAGGCGCCC TACGACTCCT CTGCCATCGA GATGACGCTG
GTGGAAGTTC TACAGAAGAA GCTGATGGCC TTCACCCTGC AGGACCTGCG GAAGGAGGCA
GACACTCCCA AGTGTGAAGA GAAGGAAAGG AGGAAGAGGG AGTTGCACAG CTCGGCCACA
GGAAGGTCTT GCCGAGGGGC ATCTGAGCAG AGGCTGGGGG ACGCGCCAAG CTGCCGTGTG
GTGGTGCACG GCGTTCAGGC CTGCAGGATG GAGGGAGTGA GCAGGGCCTG GAGGAGGCCT
CAGGACTGGG CATCTGGGGT GGAACATCCA CAGCGAGGGT GGGTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59810
Clone ID Related Accession (Same CDS sequence) XM_011534797
Accession Version XM_011534797.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5976bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA polymerase epsilon catalytic subunit A isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024477.15) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
ATGGCCAGGG AGATTGTTTC AGAAGATATT GAAGATTTTG AGTTCACCCC CAAGCCAGAA 
TATGAAGGCC CCTTTTGTGT CTTCAATGAA CCCGATGAGG CTCATCTGAT CCAAAGGTGG
TTTGAACACG TCCAGGAGAC CAAACCCACC ATCATGGTCA CCTACAACGG GGACTTTTTT
GACTGGCCAT TTGTGGAGGC CCGGGCAGCA GTCCACGGTC TGAGCATGCA GCAGGAGATA
GGCTTCCAGA AGGACAGCCA GGGGGAGTAC AAGGCGCCCC AGTGCATCCA CATGGACTGC
CTCAGGTGGG TGAAGAGGGA CAGTTACCTT CCTGTGGGCA GTCATAATCT CAAGGCGGCC
GCCAAGGCCA AGCTAGGCTA TGATCCCGTG GAGCTAGACC CGGAGGACAT GTGCCGGATG
GCCACGGAGC AGCCCCAGAC TCTGGCCACG TATTCTGTGT CAGATGCTGT CGCCACTTAC
TACCTGTACA TGAAGTACGT CCACCCATTC ATCTTTGCTC TGTGCACCAT TATTCCCATG
GAGCCCGACG AGGTGCTGCG GAAGGGCTCT GGCACTCTGT GTGAGGCCTT GCTGATGGTG
CAGGCCTTCC ACGCCAACAT CATCTTCCCC AACAAGCAAG AGCAGGAGTT CAATAAGCTG
ACGGACGACG GACACGTGCT GGACTCTGAG ACCTACGTCG GGGGCCACGT GGAGGCCCTC
GAGTCTGGGG TTTTCCGCAG CGATATCCCT TGCCGGTTTA GGATGAATCC TGCCGCCTTT
GACTTCCTGC TGCAGCGGGT TGAGAAGACC TTGCGCCACG CCCTTGAGGA AGAGGAGAAA
GTGCCTGTGG AGCAAGTCAC CAACTTTGAA GAGGTGTGTG ATGAGATTAA GAGCAAGCTT
GCCTCCCTGA AGGACGTTCC CAGCCGCATC GAGTGTCCAC TCATCTACCA CCTGGACGTG
GGGGCCATGT ACCCCAACAT CATCCTGACC AACCGCCTGC AGCCCTCTGC CATGGTGGAC
GAAGCCACCT GTGCTGCCTG TGACTTCAAT AAGCCTGGAG CAAACTGCCA GCGGAAGATG
GCCTGGCAGT GGAGGGGCGA GTTCATGCCA GCCAGTCGCA GCGAATACCA TCGGATCCAG
CACCAGCTGG AGTCAGAGAA GTTCCCCCCC TTGTTCCCAG AGGGGCCAGC TCGGGCCTTT
CATGAACTGT CCCGCGAGGA ACAGGCGAAA TACGAGAAGA GAAGGCTGGC GGATTACTGC
CGGAAAGCCT ACAAGAAGAT CCACATCACC AAGGTGGAAG AGCGTCTCAC CACCATCTGC
CAGCGGGAAA ACTCCTTCTA CGTGGACACC GTGCGTGCCT TCCGGGACAG GCGTTACGAG
TTCAAAGGGC TCCACAAGGT GTGGAAAAAG AAGCTCTCGG CGGCCGTGGA GGTGGGCGAC
GCGGCTGAGG TGAAGCGCTG CAAGAACATG GAGGTGCTGT ATGACTCGCT GCAGCTGGCC
CACAAGTGCA TCCTGAACTC CTTCTATGGC TATGTCATGC GCAAGGGGGC TCGCTGGTAC
TCCATGGAGA TGGCTGGCAT CGTCTGCTTC ACAGGGGCCA ACATCATCAC CCAGGCACGG
GAGCTGATCG AGCAGATTGG GAGGCCCTTA GAGCTGGACA CAGATGGTAT ATGGTGCGTC
CTGCCCAACA GCTTCCCAGA AAATTTTGTC TTCAAGACGA CCAATGTGAA GAAGCCCAAA
GTGACCATCT CCTACCCAGG CGCCATGTTG AACATCATGG TCAAGGAAGG CTTCACCAAT
GACCAGTACC AGGAGCTGGC TGAGCCGTCC TCACTCACCT ACGTCACCCG CTCAGAGAAC
AGCATCTTTT TTGAGGTTGA TGGGCCCTAC CTTGCCATGA TTCTTCCAGC CTCCAAGGAA
GAAGGCAAGA AATTGAAGAA GAGGTATGCT GTGTTCAATG AAGACGGTTC TCTGGCTGAG
CTCAAGGGCT TTGAGGTCAA ACGCCGCGGG GAACTGCAGC TGATTAAGAT CTTCCAATCC
TCGGTGTTTG AGGCCTTCCT CAAGGGCAGC ACGCTGGAAG AGGTGTATGG CTCTGTAGCC
AAGGTGGCTG ACTACTGGCT GGACGTGCTG TACAGCAAGG CAGCCAACAT GCCTGACTCT
GAGCTATTCG AGCTCATCTC TGAGAACCGT TCCATGTCTC GGAAGCTGGA AGATTACGGG
GAGCAGAAGT CTACGTCCAT CAGCACAGCA AAGCGCCTGG CCGAGTTCCT GGGAGACCAG
ATGGTCAAGG ATGCAGGGCT GAGTTGCCGC TACATCATCT CCCGCAAGCC CGAGGGCTCC
CCTGTCACGG AGAGGGCCAT CCCACTTGCC ATTTTCCAAG CAGAGCCCAC GGTGAGGAAG
CACTTTCTCC GGAAATGGCT CAAGAGCTCT TCCCTTCAAG ACTTTGATAT TCGAGCAATT
CTGGATTGGG ACTACTACAT TGAGCGGCTG GGAAGCGCCA TCCAGAAGAT CATCACCATC
CCTGCGGCCC TGCAGCAGGT AAAGAACCCA GTGCCACGTG TCAAACACCC CGACTGGCTG
CACAAAAAAC TGCTGGAGAA GAATGATGTC TACAAGCAGA AGAAGATCAG TGAGCTCTTC
ACCCTGGAGG GCAGGAGACA GGTCACGATG GCCGAGGCCT CAGAAGACAG TCCGAGGCCA
AGTGCTCCTG ACATGGAGGA CTTCGGCCTC GTAAAGCTGC CTCACCCAGC AGCCCCTGTC
ACTGTGAAGA GGAAGCGAGT TCTTTGGGAG AGCCAGGAGG AGTCCCAGGA CCTCACGCCG
ACTGTGCCCT GGCAGGAAAT CTTGGGGCAG CCTCCCGCCC TGGGAACCAG CCAGGAGGAA
TGGCTTGTCT GGCTCCGGTT CCACAAGAAG AAGTGGCAGC TGCAGGCCCG GCAGCGCCTC
GCCCGCAGGA AGAGGCAGCG TCTGGAGTCG GCAGAGGGTG TGCTCAGGCC CGGGGCCATC
CGGGATGGTC CTGCCACGGG GCTGGGGAGC TTCTTGCGAA GAACTGCCCG CAGCATCCTG
GACCTTCCGT GGCAGATTGT GCAGATCAGC GAGACCAGCC AGGCCGGCCT GTTCAGGCTG
TGGGCGCTCG TTGGCAGTGA CTTGCACTGC ATCAGGCTGA GCATCCCCCG TGTGTTCTAC
GTGAACCAGC GAGTCGCTAA AGCGGAGGAG GGTGCTTCGT ATCGCAAGGT AAATCGGGTC
CTTCCTCGCT CCAACATGGT CTACAATCTC TATGAGTATT CAGTGCCAGA GGACATGTAC
CAGGAACACA TCAACGAGAT CAACGCTGAG CTGTCAGCGC CAGACATCGA GGGCGTATAT
GAGACTCAGG TTCCGTTACT GTTCCGGGCC CTGGTGCACC TGGGCTGTGT GTGTGTGGTC
AATAAACAGC TGGTGAGGCA CCTTTCAGGC TGGGAAGCAG AGACCTTTGC TCTTGAGCAC
CTGGAGATGC GCTCTCTGGC CCAGTTCAGC TACCTGGAAC CAGGGAGTAT CCGCCATATC
TACCTGTACC ACCACGCACA GGCCCACAAA GCGCTCTTCG GGATCTTCAT CCCCTCACAG
CGCAGGGCAT CCGTCTTTGT GCTGGACACT GTGCGCAGCA ACCAGATGCC CAGCCTTGGC
GCCCTGTACT CAGCAGAGCA CGGCCTCCTC CTGGAGAAGG TGGGCCCTGA GCTCCTGCCA
CCCCCCAAAC ACACCTTCGA AGTTCGGGCA GAAACTGACC TGAAGACCAT CTGCAGAGCC
ATCCAGCGAT TCCTGCTCGC CTACAAGGAG GAGCGCCGGG GGCCCACACT CATCGCTGTT
CAGTCCAGCT GGGAGCTGAA GAGGCTGGCC AGTGAAATTC CTGTCTTGGA GGAATTCCCA
CTGGTGCCTA TCTGTGTGGC TGACAAGATC AACTATGGGG TCCTGGACTG GCAGCGCCAT
GGAGCCCGGC GCATGATCCG TCACTACCTC AACCTGGACA CCTGCCTGTC GCAGGCCTTC
GAGATGAGCA GGTACTTTCA CATTCCCATT GGGAACCTAC CAGAGGACAT CTCCACATTC
GGCTCCGACC TCTTCTTTGC CCGCCACCTC CAGCGCCACA ACCACCTGCT CTGGCTGTCC
CCTACAGCCC GCCCTGACCT GGGTGGAAAG GAGGCTGATG ACAACTGTCT TGTCATGGAG
TTCGATGACC AAGCCACTGT TGAGATCAAC AGTTCAGGCT GTTACTCCAC AGTGTGTGTG
GAGCTGGACC TTCAGAACCT GGCCGTCAAC ACCATTCTCC AGTCTCACCA TGTCAACGAC
ATGGAGGGGG CCGACAGCAT GGGGATCAGC TTCGACGTGA TCCAGCAGGC CTCCCTGGAG
GACATGATCA CGGGTGGTCA GGCTGCCAGT GCCCCGGCCA GCTACGATGA GACAGCCCTG
TGCTCTAACA CCTTCAGGAT CCTGAAGAGC ATGGTCGTGG GCTGGGTGAA GGAGATCACC
CAGTACCACA ACATCTATGC AGACAACCAG GTGATGCACT TCTACCGCTG GCTTCGGTCG
CCATCCTCTC TGCTTCATGA CCCTGCCCTG CACCGCACAC TCCACAACAT GATGAAGAAG
CTCTTCCTGC AGCTCATCGC TGAGTTCAAG CGCCTGGGGT CATCAGTCAT CTACGCCAAC
TTCAACCGCA TCATCCTCTG TACAAAGAAG CGCCGTGTGG AAGATGCCAT CGCTTACGTG
GAGTACATCA CCAGCAGCAT CCATTCAAAG GAGACCTTCC ATTCTCTGAC AATTTCTTTC
TCTCGATGCT GGGAATTTCT TCTCTGGATG GATCCATCTA ACTATGGCGG AATCAAAGGA
AAAGTTTCAT CTCGTATTCA CTGTGGACTG CAAGACTCCC AGAAAGCAGG GGGAGCAGAG
GATGAGCAGG AAAATGAGGA CGATGAGGAG GAAAGAGATG GGGAGGAGGA GGAAGAGGCG
GAGGAATCCA ACGTGGAGGA TTTACTGGAA AACAACTGGA ACATTTTGCA GTTTTTGCCA
CAGGCAGCCT CCTGCCAGAA CTACTTCCTC ATGATTGTTT CAGCGTACAT CGTGGCCGTG
TACCACTGCA TGAAGGACGG GCTGAGGCGC AGTGCTCCAG GGAGCACCCC CGTGAGGAGG
AGGGGGGCCA GCCAGCTCTC CCAGGAGGCC GAGGGGGCGG TCGGAGCCCT TCCCGGAATG
ATCACCTTCT CTCAGGATTA TGTCGCAAAT GAGCTCACTC AGAGCTTCTT CACCATCACT
CAGAAGATTC AGAAGAAAGT CACAGGCTCT CGGAACTCCA CTGAGCTCTC AGAGATGTTT
CCTGTCCTCC CCGGTTCCCA CTTGCTGCTC AATAACCCTG CCCTGGAGTT CATCAAATAC
GTGTGCAAGG TGCTGTCCCT GGACACCAAC ATCACAAACC AGGTGAATAA GCTGAACCGA
GACCTGCTTC GCCTGGTGGA TGTCGGCGAG TTCTCCGAGG AGGCCCAGTT CCGAGACCCC
TGCCGCTCCT ACGTGCTTCC TGAGGTCATC TGCCGCAGCT GTAACTTCTG CCGCGACCTG
GACCTGTGTA AAGACTCTTC CTTCTCAGAG GATGGGGCGG TCCTGCCTCA GTGGCTCTGC
TCCAACTGTC AGGCGCCCTA CGACTCCTCT GCCATCGAGA TGACGCTGGT GGAAGTTCTA
CAGAAGAAGC TGATGGCCTT CACCCTGCAG GACCTGCGGA AGGAGGCAGA CACTCCCAAG
TGTGAAGAGA AGGAAAGGAG GAAGAGGGAG TTGCACAGCT CGGCCACAGG AAGGTCTTGC
CGAGGGGCAT CTGAGCAGAG GCTGGGGGAC GCGCCAAGCT GCCGTGTGGT GGTGCACGGC
GTTCAGGCCT GCAGGATGGA GGGAGTGAGC AGGGCCTGGA GGAGGCCTCA GGACTGGGCA
TCTGGGGTGG AACATCCACA GCGAGGGTGG GTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533099.1
CDS87..6062
Misc Feature(1)93..578(+)
Misc Feature(2)108..1775(+)
Misc Feature(3)249..551(+)
Misc Feature(4)249..551(+)
Misc Feature(5)267..551(+)
Misc Feature(6)756..2624(+)
Misc Feature(7)1041..1751(+)
Misc Feature(8)1743..1754(+)
Misc Feature(9)3738..4940(+)
Translation

Target ORF information:

RefSeq Version XM_011534797
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011534797

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
ATGGCCAGGG AGATTGTTTC AGAAGATATT GAAGATTTTG AGTTCACCCC CAAGCCAGAA 
TATGAAGGCC CCTTTTGTGT CTTCAATGAA CCCGATGAGG CTCATCTGAT CCAAAGGTGG
TTTGAACACG TCCAGGAGAC CAAACCCACC ATCATGGTCA CCTACAACGG GGACTTTTTT
GACTGGCCAT TTGTGGAGGC CCGGGCAGCA GTCCACGGTC TGAGCATGCA GCAGGAGATA
GGCTTCCAGA AGGACAGCCA GGGGGAGTAC AAGGCGCCCC AGTGCATCCA CATGGACTGC
CTCAGGTGGG TGAAGAGGGA CAGTTACCTT CCTGTGGGCA GTCATAATCT CAAGGCGGCC
GCCAAGGCCA AGCTAGGCTA TGATCCCGTG GAGCTAGACC CGGAGGACAT GTGCCGGATG
GCCACGGAGC AGCCCCAGAC TCTGGCCACG TATTCTGTGT CAGATGCTGT CGCCACTTAC
TACCTGTACA TGAAGTACGT CCACCCATTC ATCTTTGCTC TGTGCACCAT TATTCCCATG
GAGCCCGACG AGGTGCTGCG GAAGGGCTCT GGCACTCTGT GTGAGGCCTT GCTGATGGTG
CAGGCCTTCC ACGCCAACAT CATCTTCCCC AACAAGCAAG AGCAGGAGTT CAATAAGCTG
ACGGACGACG GACACGTGCT GGACTCTGAG ACCTACGTCG GGGGCCACGT GGAGGCCCTC
GAGTCTGGGG TTTTCCGCAG CGATATCCCT TGCCGGTTTA GGATGAATCC TGCCGCCTTT
GACTTCCTGC TGCAGCGGGT TGAGAAGACC TTGCGCCACG CCCTTGAGGA AGAGGAGAAA
GTGCCTGTGG AGCAAGTCAC CAACTTTGAA GAGGTGTGTG ATGAGATTAA GAGCAAGCTT
GCCTCCCTGA AGGACGTTCC CAGCCGCATC GAGTGTCCAC TCATCTACCA CCTGGACGTG
GGGGCCATGT ACCCCAACAT CATCCTGACC AACCGCCTGC AGCCCTCTGC CATGGTGGAC
GAAGCCACCT GTGCTGCCTG TGACTTCAAT AAGCCTGGAG CAAACTGCCA GCGGAAGATG
GCCTGGCAGT GGAGGGGCGA GTTCATGCCA GCCAGTCGCA GCGAATACCA TCGGATCCAG
CACCAGCTGG AGTCAGAGAA GTTCCCCCCC TTGTTCCCAG AGGGGCCAGC TCGGGCCTTT
CATGAACTGT CCCGCGAGGA ACAGGCGAAA TACGAGAAGA GAAGGCTGGC GGATTACTGC
CGGAAAGCCT ACAAGAAGAT CCACATCACC AAGGTGGAAG AGCGTCTCAC CACCATCTGC
CAGCGGGAAA ACTCCTTCTA CGTGGACACC GTGCGTGCCT TCCGGGACAG GCGTTACGAG
TTCAAAGGGC TCCACAAGGT GTGGAAAAAG AAGCTCTCGG CGGCCGTGGA GGTGGGCGAC
GCGGCTGAGG TGAAGCGCTG CAAGAACATG GAGGTGCTGT ATGACTCGCT GCAGCTGGCC
CACAAGTGCA TCCTGAACTC CTTCTATGGC TATGTCATGC GCAAGGGGGC TCGCTGGTAC
TCCATGGAGA TGGCTGGCAT CGTCTGCTTC ACAGGGGCCA ACATCATCAC CCAGGCACGG
GAGCTGATCG AGCAGATTGG GAGGCCCTTA GAGCTGGACA CAGATGGTAT ATGGTGCGTC
CTGCCCAACA GCTTCCCAGA AAATTTTGTC TTCAAGACGA CCAATGTGAA GAAGCCCAAA
GTGACCATCT CCTACCCAGG CGCCATGTTG AACATCATGG TCAAGGAAGG CTTCACCAAT
GACCAGTACC AGGAGCTGGC TGAGCCGTCC TCACTCACCT ACGTCACCCG CTCAGAGAAC
AGCATCTTTT TTGAGGTTGA TGGGCCCTAC CTTGCCATGA TTCTTCCAGC CTCCAAGGAA
GAAGGCAAGA AATTGAAGAA GAGGTATGCT GTGTTCAATG AAGACGGTTC TCTGGCTGAG
CTCAAGGGCT TTGAGGTCAA ACGCCGCGGG GAACTGCAGC TGATTAAGAT CTTCCAATCC
TCGGTGTTTG AGGCCTTCCT CAAGGGCAGC ACGCTGGAAG AGGTGTATGG CTCTGTAGCC
AAGGTGGCTG ACTACTGGCT GGACGTGCTG TACAGCAAGG CAGCCAACAT GCCTGACTCT
GAGCTATTCG AGCTCATCTC TGAGAACCGT TCCATGTCTC GGAAGCTGGA AGATTACGGG
GAGCAGAAGT CTACGTCCAT CAGCACAGCA AAGCGCCTGG CCGAGTTCCT GGGAGACCAG
ATGGTCAAGG ATGCAGGGCT GAGTTGCCGC TACATCATCT CCCGCAAGCC CGAGGGCTCC
CCTGTCACGG AGAGGGCCAT CCCACTTGCC ATTTTCCAAG CAGAGCCCAC GGTGAGGAAG
CACTTTCTCC GGAAATGGCT CAAGAGCTCT TCCCTTCAAG ACTTTGATAT TCGAGCAATT
CTGGATTGGG ACTACTACAT TGAGCGGCTG GGAAGCGCCA TCCAGAAGAT CATCACCATC
CCTGCGGCCC TGCAGCAGGT AAAGAACCCA GTGCCACGTG TCAAACACCC CGACTGGCTG
CACAAAAAAC TGCTGGAGAA GAATGATGTC TACAAGCAGA AGAAGATCAG TGAGCTCTTC
ACCCTGGAGG GCAGGAGACA GGTCACGATG GCCGAGGCCT CAGAAGACAG TCCGAGGCCA
AGTGCTCCTG ACATGGAGGA CTTCGGCCTC GTAAAGCTGC CTCACCCAGC AGCCCCTGTC
ACTGTGAAGA GGAAGCGAGT TCTTTGGGAG AGCCAGGAGG AGTCCCAGGA CCTCACGCCG
ACTGTGCCCT GGCAGGAAAT CTTGGGGCAG CCTCCCGCCC TGGGAACCAG CCAGGAGGAA
TGGCTTGTCT GGCTCCGGTT CCACAAGAAG AAGTGGCAGC TGCAGGCCCG GCAGCGCCTC
GCCCGCAGGA AGAGGCAGCG TCTGGAGTCG GCAGAGGGTG TGCTCAGGCC CGGGGCCATC
CGGGATGGTC CTGCCACGGG GCTGGGGAGC TTCTTGCGAA GAACTGCCCG CAGCATCCTG
GACCTTCCGT GGCAGATTGT GCAGATCAGC GAGACCAGCC AGGCCGGCCT GTTCAGGCTG
TGGGCGCTCG TTGGCAGTGA CTTGCACTGC ATCAGGCTGA GCATCCCCCG TGTGTTCTAC
GTGAACCAGC GAGTCGCTAA AGCGGAGGAG GGTGCTTCGT ATCGCAAGGT AAATCGGGTC
CTTCCTCGCT CCAACATGGT CTACAATCTC TATGAGTATT CAGTGCCAGA GGACATGTAC
CAGGAACACA TCAACGAGAT CAACGCTGAG CTGTCAGCGC CAGACATCGA GGGCGTATAT
GAGACTCAGG TTCCGTTACT GTTCCGGGCC CTGGTGCACC TGGGCTGTGT GTGTGTGGTC
AATAAACAGC TGGTGAGGCA CCTTTCAGGC TGGGAAGCAG AGACCTTTGC TCTTGAGCAC
CTGGAGATGC GCTCTCTGGC CCAGTTCAGC TACCTGGAAC CAGGGAGTAT CCGCCATATC
TACCTGTACC ACCACGCACA GGCCCACAAA GCGCTCTTCG GGATCTTCAT CCCCTCACAG
CGCAGGGCAT CCGTCTTTGT GCTGGACACT GTGCGCAGCA ACCAGATGCC CAGCCTTGGC
GCCCTGTACT CAGCAGAGCA CGGCCTCCTC CTGGAGAAGG TGGGCCCTGA GCTCCTGCCA
CCCCCCAAAC ACACCTTCGA AGTTCGGGCA GAAACTGACC TGAAGACCAT CTGCAGAGCC
ATCCAGCGAT TCCTGCTCGC CTACAAGGAG GAGCGCCGGG GGCCCACACT CATCGCTGTT
CAGTCCAGCT GGGAGCTGAA GAGGCTGGCC AGTGAAATTC CTGTCTTGGA GGAATTCCCA
CTGGTGCCTA TCTGTGTGGC TGACAAGATC AACTATGGGG TCCTGGACTG GCAGCGCCAT
GGAGCCCGGC GCATGATCCG TCACTACCTC AACCTGGACA CCTGCCTGTC GCAGGCCTTC
GAGATGAGCA GGTACTTTCA CATTCCCATT GGGAACCTAC CAGAGGACAT CTCCACATTC
GGCTCCGACC TCTTCTTTGC CCGCCACCTC CAGCGCCACA ACCACCTGCT CTGGCTGTCC
CCTACAGCCC GCCCTGACCT GGGTGGAAAG GAGGCTGATG ACAACTGTCT TGTCATGGAG
TTCGATGACC AAGCCACTGT TGAGATCAAC AGTTCAGGCT GTTACTCCAC AGTGTGTGTG
GAGCTGGACC TTCAGAACCT GGCCGTCAAC ACCATTCTCC AGTCTCACCA TGTCAACGAC
ATGGAGGGGG CCGACAGCAT GGGGATCAGC TTCGACGTGA TCCAGCAGGC CTCCCTGGAG
GACATGATCA CGGGTGGTCA GGCTGCCAGT GCCCCGGCCA GCTACGATGA GACAGCCCTG
TGCTCTAACA CCTTCAGGAT CCTGAAGAGC ATGGTCGTGG GCTGGGTGAA GGAGATCACC
CAGTACCACA ACATCTATGC AGACAACCAG GTGATGCACT TCTACCGCTG GCTTCGGTCG
CCATCCTCTC TGCTTCATGA CCCTGCCCTG CACCGCACAC TCCACAACAT GATGAAGAAG
CTCTTCCTGC AGCTCATCGC TGAGTTCAAG CGCCTGGGGT CATCAGTCAT CTACGCCAAC
TTCAACCGCA TCATCCTCTG TACAAAGAAG CGCCGTGTGG AAGATGCCAT CGCTTACGTG
GAGTACATCA CCAGCAGCAT CCATTCAAAG GAGACCTTCC ATTCTCTGAC AATTTCTTTC
TCTCGATGCT GGGAATTTCT TCTCTGGATG GATCCATCTA ACTATGGCGG AATCAAAGGA
AAAGTTTCAT CTCGTATTCA CTGTGGACTG CAAGACTCCC AGAAAGCAGG GGGAGCAGAG
GATGAGCAGG AAAATGAGGA CGATGAGGAG GAAAGAGATG GGGAGGAGGA GGAAGAGGCG
GAGGAATCCA ACGTGGAGGA TTTACTGGAA AACAACTGGA ACATTTTGCA GTTTTTGCCA
CAGGCAGCCT CCTGCCAGAA CTACTTCCTC ATGATTGTTT CAGCGTACAT CGTGGCCGTG
TACCACTGCA TGAAGGACGG GCTGAGGCGC AGTGCTCCAG GGAGCACCCC CGTGAGGAGG
AGGGGGGCCA GCCAGCTCTC CCAGGAGGCC GAGGGGGCGG TCGGAGCCCT TCCCGGAATG
ATCACCTTCT CTCAGGATTA TGTCGCAAAT GAGCTCACTC AGAGCTTCTT CACCATCACT
CAGAAGATTC AGAAGAAAGT CACAGGCTCT CGGAACTCCA CTGAGCTCTC AGAGATGTTT
CCTGTCCTCC CCGGTTCCCA CTTGCTGCTC AATAACCCTG CCCTGGAGTT CATCAAATAC
GTGTGCAAGG TGCTGTCCCT GGACACCAAC ATCACAAACC AGGTGAATAA GCTGAACCGA
GACCTGCTTC GCCTGGTGGA TGTCGGCGAG TTCTCCGAGG AGGCCCAGTT CCGAGACCCC
TGCCGCTCCT ACGTGCTTCC TGAGGTCATC TGCCGCAGCT GTAACTTCTG CCGCGACCTG
GACCTGTGTA AAGACTCTTC CTTCTCAGAG GATGGGGCGG TCCTGCCTCA GTGGCTCTGC
TCCAACTGTC AGGCGCCCTA CGACTCCTCT GCCATCGAGA TGACGCTGGT GGAAGTTCTA
CAGAAGAAGC TGATGGCCTT CACCCTGCAG GACCTGCGGA AGGAGGCAGA CACTCCCAAG
TGTGAAGAGA AGGAAAGGAG GAAGAGGGAG TTGCACAGCT CGGCCACAGG AAGGTCTTGC
CGAGGGGCAT CTGAGCAGAG GCTGGGGGAC GCGCCAAGCT GCCGTGTGGT GGTGCACGGC
GTTCAGGCCT GCAGGATGGA GGGAGTGAGC AGGGCCTGGA GGAGGCCTCA GGACTGGGCA
TCTGGGGTGG AACATCCACA GCGAGGGTGG GTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59811
Clone ID Related Accession (Same CDS sequence) XM_011534798
Accession Version XM_011534798.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5559bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA polymerase epsilon catalytic subunit A isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024477.15) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
ATGGCCACGG AGCAGCCCCA GACTCTGGCC ACGTATTCTG TGTCAGATGC TGTCGCCACT 
TACTACCTGT ACATGAAGTA CGTCCACCCA TTCATCTTTG CTCTGTGCAC CATTATTCCC
ATGGAGCCCG ACGAGGTGCT GCGGAAGGGC TCTGGCACTC TGTGTGAGGC CTTGCTGATG
GTGCAGGCCT TCCACGCCAA CATCATCTTC CCCAACAAGC AAGAGCAGGA GTTCAATAAG
CTGACGGACG ACGGACACGT GCTGGACTCT GAGACCTACG TCGGGGGCCA CGTGGAGGCC
CTCGAGTCTG GGGTTTTCCG CAGCGATATC CCTTGCCGGT TTAGGATGAA TCCTGCCGCC
TTTGACTTCC TGCTGCAGCG GGTTGAGAAG ACCTTGCGCC ACGCCCTTGA GGAAGAGGAG
AAAGTGCCTG TGGAGCAAGT CACCAACTTT GAAGAGGTGT GTGATGAGAT TAAGAGCAAG
CTTGCCTCCC TGAAGGACGT TCCCAGCCGC ATCGAGTGTC CACTCATCTA CCACCTGGAC
GTGGGGGCCA TGTACCCCAA CATCATCCTG ACCAACCGCC TGCAGCCCTC TGCCATGGTG
GACGAAGCCA CCTGTGCTGC CTGTGACTTC AATAAGCCTG GAGCAAACTG CCAGCGGAAG
ATGGCCTGGC AGTGGAGGGG CGAGTTCATG CCAGCCAGTC GCAGCGAATA CCATCGGATC
CAGCACCAGC TGGAGTCAGA GAAGTTCCCC CCCTTGTTCC CAGAGGGGCC AGCTCGGGCC
TTTCATGAAC TGTCCCGCGA GGAACAGGCG AAATACGAGA AGAGAAGGCT GGCGGATTAC
TGCCGGAAAG CCTACAAGAA GATCCACATC ACCAAGGTGG AAGAGCGTCT CACCACCATC
TGCCAGCGGG AAAACTCCTT CTACGTGGAC ACCGTGCGTG CCTTCCGGGA CAGGCGTTAC
GAGTTCAAAG GGCTCCACAA GGTGTGGAAA AAGAAGCTCT CGGCGGCCGT GGAGGTGGGC
GACGCGGCTG AGGTGAAGCG CTGCAAGAAC ATGGAGGTGC TGTATGACTC GCTGCAGCTG
GCCCACAAGT GCATCCTGAA CTCCTTCTAT GGCTATGTCA TGCGCAAGGG GGCTCGCTGG
TACTCCATGG AGATGGCTGG CATCGTCTGC TTCACAGGGG CCAACATCAT CACCCAGGCA
CGGGAGCTGA TCGAGCAGAT TGGGAGGCCC TTAGAGCTGG ACACAGATGG TATATGGTGC
GTCCTGCCCA ACAGCTTCCC AGAAAATTTT GTCTTCAAGA CGACCAATGT GAAGAAGCCC
AAAGTGACCA TCTCCTACCC AGGCGCCATG TTGAACATCA TGGTCAAGGA AGGCTTCACC
AATGACCAGT ACCAGGAGCT GGCTGAGCCG TCCTCACTCA CCTACGTCAC CCGCTCAGAG
AACAGCATCT TTTTTGAGGT TGATGGGCCC TACCTTGCCA TGATTCTTCC AGCCTCCAAG
GAAGAAGGCA AGAAATTGAA GAAGAGGTAT GCTGTGTTCA ATGAAGACGG TTCTCTGGCT
GAGCTCAAGG GCTTTGAGGT CAAACGCCGC GGGGAACTGC AGCTGATTAA GATCTTCCAA
TCCTCGGTGT TTGAGGCCTT CCTCAAGGGC AGCACGCTGG AAGAGGTGTA TGGCTCTGTA
GCCAAGGTGG CTGACTACTG GCTGGACGTG CTGTACAGCA AGGCAGCCAA CATGCCTGAC
TCTGAGCTAT TCGAGCTCAT CTCTGAGAAC CGTTCCATGT CTCGGAAGCT GGAAGATTAC
GGGGAGCAGA AGTCTACGTC CATCAGCACA GCAAAGCGCC TGGCCGAGTT CCTGGGAGAC
CAGATGGTCA AGGATGCAGG GCTGAGTTGC CGCTACATCA TCTCCCGCAA GCCCGAGGGC
TCCCCTGTCA CGGAGAGGGC CATCCCACTT GCCATTTTCC AAGCAGAGCC CACGGTGAGG
AAGCACTTTC TCCGGAAATG GCTCAAGAGC TCTTCCCTTC AAGACTTTGA TATTCGAGCA
ATTCTGGATT GGGACTACTA CATTGAGCGG CTGGGAAGCG CCATCCAGAA GATCATCACC
ATCCCTGCGG CCCTGCAGCA GGTAAAGAAC CCAGTGCCAC GTGTCAAACA CCCCGACTGG
CTGCACAAAA AACTGCTGGA GAAGAATGAT GTCTACAAGC AGAAGAAGAT CAGTGAGCTC
TTCACCCTGG AGGGCAGGAG ACAGGTCACG ATGGCCGAGG CCTCAGAAGA CAGTCCGAGG
CCAAGTGCTC CTGACATGGA GGACTTCGGC CTCGTAAAGC TGCCTCACCC AGCAGCCCCT
GTCACTGTGA AGAGGAAGCG AGTTCTTTGG GAGAGCCAGG AGGAGTCCCA GGACCTCACG
CCGACTGTGC CCTGGCAGGA AATCTTGGGG CAGCCTCCCG CCCTGGGAAC CAGCCAGGAG
GAATGGCTTG TCTGGCTCCG GTTCCACAAG AAGAAGTGGC AGCTGCAGGC CCGGCAGCGC
CTCGCCCGCA GGAAGAGGCA GCGTCTGGAG TCGGCAGAGG GTGTGCTCAG GCCCGGGGCC
ATCCGGGATG GTCCTGCCAC GGGGCTGGGG AGCTTCTTGC GAAGAACTGC CCGCAGCATC
CTGGACCTTC CGTGGCAGAT TGTGCAGATC AGCGAGACCA GCCAGGCCGG CCTGTTCAGG
CTGTGGGCGC TCGTTGGCAG TGACTTGCAC TGCATCAGGC TGAGCATCCC CCGTGTGTTC
TACGTGAACC AGCGAGTCGC TAAAGCGGAG GAGGGTGCTT CGTATCGCAA GGTAAATCGG
GTCCTTCCTC GCTCCAACAT GGTCTACAAT CTCTATGAGT ATTCAGTGCC AGAGGACATG
TACCAGGAAC ACATCAACGA GATCAACGCT GAGCTGTCAG CGCCAGACAT CGAGGGCGTA
TATGAGACTC AGGTTCCGTT ACTGTTCCGG GCCCTGGTGC ACCTGGGCTG TGTGTGTGTG
GTCAATAAAC AGCTGGTGAG GCACCTTTCA GGCTGGGAAG CAGAGACCTT TGCTCTTGAG
CACCTGGAGA TGCGCTCTCT GGCCCAGTTC AGCTACCTGG AACCAGGGAG TATCCGCCAT
ATCTACCTGT ACCACCACGC ACAGGCCCAC AAAGCGCTCT TCGGGATCTT CATCCCCTCA
CAGCGCAGGG CATCCGTCTT TGTGCTGGAC ACTGTGCGCA GCAACCAGAT GCCCAGCCTT
GGCGCCCTGT ACTCAGCAGA GCACGGCCTC CTCCTGGAGA AGGTGGGCCC TGAGCTCCTG
CCACCCCCCA AACACACCTT CGAAGTTCGG GCAGAAACTG ACCTGAAGAC CATCTGCAGA
GCCATCCAGC GATTCCTGCT CGCCTACAAG GAGGAGCGCC GGGGGCCCAC ACTCATCGCT
GTTCAGTCCA GCTGGGAGCT GAAGAGGCTG GCCAGTGAAA TTCCTGTCTT GGAGGAATTC
CCACTGGTGC CTATCTGTGT GGCTGACAAG ATCAACTATG GGGTCCTGGA CTGGCAGCGC
CATGGAGCCC GGCGCATGAT CCGTCACTAC CTCAACCTGG ACACCTGCCT GTCGCAGGCC
TTCGAGATGA GCAGGTACTT TCACATTCCC ATTGGGAACC TACCAGAGGA CATCTCCACA
TTCGGCTCCG ACCTCTTCTT TGCCCGCCAC CTCCAGCGCC ACAACCACCT GCTCTGGCTG
TCCCCTACAG CCCGCCCTGA CCTGGGTGGA AAGGAGGCTG ATGACAACTG TCTTGTCATG
GAGTTCGATG ACCAAGCCAC TGTTGAGATC AACAGTTCAG GCTGTTACTC CACAGTGTGT
GTGGAGCTGG ACCTTCAGAA CCTGGCCGTC AACACCATTC TCCAGTCTCA CCATGTCAAC
GACATGGAGG GGGCCGACAG CATGGGGATC AGCTTCGACG TGATCCAGCA GGCCTCCCTG
GAGGACATGA TCACGGGTGG TCAGGCTGCC AGTGCCCCGG CCAGCTACGA TGAGACAGCC
CTGTGCTCTA ACACCTTCAG GATCCTGAAG AGCATGGTCG TGGGCTGGGT GAAGGAGATC
ACCCAGTACC ACAACATCTA TGCAGACAAC CAGGTGATGC ACTTCTACCG CTGGCTTCGG
TCGCCATCCT CTCTGCTTCA TGACCCTGCC CTGCACCGCA CACTCCACAA CATGATGAAG
AAGCTCTTCC TGCAGCTCAT CGCTGAGTTC AAGCGCCTGG GGTCATCAGT CATCTACGCC
AACTTCAACC GCATCATCCT CTGTACAAAG AAGCGCCGTG TGGAAGATGC CATCGCTTAC
GTGGAGTACA TCACCAGCAG CATCCATTCA AAGGAGACCT TCCATTCTCT GACAATTTCT
TTCTCTCGAT GCTGGGAATT TCTTCTCTGG ATGGATCCAT CTAACTATGG CGGAATCAAA
GGAAAAGTTT CATCTCGTAT TCACTGTGGA CTGCAAGACT CCCAGAAAGC AGGGGGAGCA
GAGGATGAGC AGGAAAATGA GGACGATGAG GAGGAAAGAG ATGGGGAGGA GGAGGAAGAG
GCGGAGGAAT CCAACGTGGA GGATTTACTG GAAAACAACT GGAACATTTT GCAGTTTTTG
CCACAGGCAG CCTCCTGCCA GAACTACTTC CTCATGATTG TTTCAGCGTA CATCGTGGCC
GTGTACCACT GCATGAAGGA CGGGCTGAGG CGCAGTGCTC CAGGGAGCAC CCCCGTGAGG
AGGAGGGGGG CCAGCCAGCT CTCCCAGGAG GCCGAGGGGG CGGTCGGAGC CCTTCCCGGA
ATGATCACCT TCTCTCAGGA TTATGTCGCA AATGAGCTCA CTCAGAGCTT CTTCACCATC
ACTCAGAAGA TTCAGAAGAA AGTCACAGGC TCTCGGAACT CCACTGAGCT CTCAGAGATG
TTTCCTGTCC TCCCCGGTTC CCACTTGCTG CTCAATAACC CTGCCCTGGA GTTCATCAAA
TACGTGTGCA AGGTGCTGTC CCTGGACACC AACATCACAA ACCAGGTGAA TAAGCTGAAC
CGAGACCTGC TTCGCCTGGT GGATGTCGGC GAGTTCTCCG AGGAGGCCCA GTTCCGAGAC
CCCTGCCGCT CCTACGTGCT TCCTGAGGTC ATCTGCCGCA GCTGTAACTT CTGCCGCGAC
CTGGACCTGT GTAAAGACTC TTCCTTCTCA GAGGATGGGG CGGTCCTGCC TCAGTGGCTC
TGCTCCAACT GTCAGGCGCC CTACGACTCC TCTGCCATCG AGATGACGCT GGTGGAAGTT
CTACAGAAGA AGCTGATGGC CTTCACCCTG CAGGACCTGC GGAAGGAGGC AGACACTCCC
AAGTGTGAAG AGAAGGAAAG GAGGAAGAGG GAGTTGCACA GCTCGGCCAC AGGAAGGTCT
TGCCGAGGGG CATCTGAGCA GAGGCTGGGG GACGCGCCAA GCTGCCGTGT GGTGGTGCAC
GGCGTTCAGG CCTGCAGGAT GGAGGGAGTG AGCAGGGCCT GGAGGAGGCC TCAGGACTGG
GCATCTGGGG TGGAACATCC ACAGCGAGGG TGGGTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533100.1
CDS64..5622
Misc Feature(1)64..138(+)
Misc Feature(2)79..2220(+)
Misc Feature(3)316..2184(+)
Misc Feature(4)601..1311(+)
Misc Feature(5)1303..1314(+)
Misc Feature(6)3298..4500(+)
Translation

Target ORF information:

RefSeq Version XM_011534798
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011534798

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
ATGGCCACGG AGCAGCCCCA GACTCTGGCC ACGTATTCTG TGTCAGATGC TGTCGCCACT 
TACTACCTGT ACATGAAGTA CGTCCACCCA TTCATCTTTG CTCTGTGCAC CATTATTCCC
ATGGAGCCCG ACGAGGTGCT GCGGAAGGGC TCTGGCACTC TGTGTGAGGC CTTGCTGATG
GTGCAGGCCT TCCACGCCAA CATCATCTTC CCCAACAAGC AAGAGCAGGA GTTCAATAAG
CTGACGGACG ACGGACACGT GCTGGACTCT GAGACCTACG TCGGGGGCCA CGTGGAGGCC
CTCGAGTCTG GGGTTTTCCG CAGCGATATC CCTTGCCGGT TTAGGATGAA TCCTGCCGCC
TTTGACTTCC TGCTGCAGCG GGTTGAGAAG ACCTTGCGCC ACGCCCTTGA GGAAGAGGAG
AAAGTGCCTG TGGAGCAAGT CACCAACTTT GAAGAGGTGT GTGATGAGAT TAAGAGCAAG
CTTGCCTCCC TGAAGGACGT TCCCAGCCGC ATCGAGTGTC CACTCATCTA CCACCTGGAC
GTGGGGGCCA TGTACCCCAA CATCATCCTG ACCAACCGCC TGCAGCCCTC TGCCATGGTG
GACGAAGCCA CCTGTGCTGC CTGTGACTTC AATAAGCCTG GAGCAAACTG CCAGCGGAAG
ATGGCCTGGC AGTGGAGGGG CGAGTTCATG CCAGCCAGTC GCAGCGAATA CCATCGGATC
CAGCACCAGC TGGAGTCAGA GAAGTTCCCC CCCTTGTTCC CAGAGGGGCC AGCTCGGGCC
TTTCATGAAC TGTCCCGCGA GGAACAGGCG AAATACGAGA AGAGAAGGCT GGCGGATTAC
TGCCGGAAAG CCTACAAGAA GATCCACATC ACCAAGGTGG AAGAGCGTCT CACCACCATC
TGCCAGCGGG AAAACTCCTT CTACGTGGAC ACCGTGCGTG CCTTCCGGGA CAGGCGTTAC
GAGTTCAAAG GGCTCCACAA GGTGTGGAAA AAGAAGCTCT CGGCGGCCGT GGAGGTGGGC
GACGCGGCTG AGGTGAAGCG CTGCAAGAAC ATGGAGGTGC TGTATGACTC GCTGCAGCTG
GCCCACAAGT GCATCCTGAA CTCCTTCTAT GGCTATGTCA TGCGCAAGGG GGCTCGCTGG
TACTCCATGG AGATGGCTGG CATCGTCTGC TTCACAGGGG CCAACATCAT CACCCAGGCA
CGGGAGCTGA TCGAGCAGAT TGGGAGGCCC TTAGAGCTGG ACACAGATGG TATATGGTGC
GTCCTGCCCA ACAGCTTCCC AGAAAATTTT GTCTTCAAGA CGACCAATGT GAAGAAGCCC
AAAGTGACCA TCTCCTACCC AGGCGCCATG TTGAACATCA TGGTCAAGGA AGGCTTCACC
AATGACCAGT ACCAGGAGCT GGCTGAGCCG TCCTCACTCA CCTACGTCAC CCGCTCAGAG
AACAGCATCT TTTTTGAGGT TGATGGGCCC TACCTTGCCA TGATTCTTCC AGCCTCCAAG
GAAGAAGGCA AGAAATTGAA GAAGAGGTAT GCTGTGTTCA ATGAAGACGG TTCTCTGGCT
GAGCTCAAGG GCTTTGAGGT CAAACGCCGC GGGGAACTGC AGCTGATTAA GATCTTCCAA
TCCTCGGTGT TTGAGGCCTT CCTCAAGGGC AGCACGCTGG AAGAGGTGTA TGGCTCTGTA
GCCAAGGTGG CTGACTACTG GCTGGACGTG CTGTACAGCA AGGCAGCCAA CATGCCTGAC
TCTGAGCTAT TCGAGCTCAT CTCTGAGAAC CGTTCCATGT CTCGGAAGCT GGAAGATTAC
GGGGAGCAGA AGTCTACGTC CATCAGCACA GCAAAGCGCC TGGCCGAGTT CCTGGGAGAC
CAGATGGTCA AGGATGCAGG GCTGAGTTGC CGCTACATCA TCTCCCGCAA GCCCGAGGGC
TCCCCTGTCA CGGAGAGGGC CATCCCACTT GCCATTTTCC AAGCAGAGCC CACGGTGAGG
AAGCACTTTC TCCGGAAATG GCTCAAGAGC TCTTCCCTTC AAGACTTTGA TATTCGAGCA
ATTCTGGATT GGGACTACTA CATTGAGCGG CTGGGAAGCG CCATCCAGAA GATCATCACC
ATCCCTGCGG CCCTGCAGCA GGTAAAGAAC CCAGTGCCAC GTGTCAAACA CCCCGACTGG
CTGCACAAAA AACTGCTGGA GAAGAATGAT GTCTACAAGC AGAAGAAGAT CAGTGAGCTC
TTCACCCTGG AGGGCAGGAG ACAGGTCACG ATGGCCGAGG CCTCAGAAGA CAGTCCGAGG
CCAAGTGCTC CTGACATGGA GGACTTCGGC CTCGTAAAGC TGCCTCACCC AGCAGCCCCT
GTCACTGTGA AGAGGAAGCG AGTTCTTTGG GAGAGCCAGG AGGAGTCCCA GGACCTCACG
CCGACTGTGC CCTGGCAGGA AATCTTGGGG CAGCCTCCCG CCCTGGGAAC CAGCCAGGAG
GAATGGCTTG TCTGGCTCCG GTTCCACAAG AAGAAGTGGC AGCTGCAGGC CCGGCAGCGC
CTCGCCCGCA GGAAGAGGCA GCGTCTGGAG TCGGCAGAGG GTGTGCTCAG GCCCGGGGCC
ATCCGGGATG GTCCTGCCAC GGGGCTGGGG AGCTTCTTGC GAAGAACTGC CCGCAGCATC
CTGGACCTTC CGTGGCAGAT TGTGCAGATC AGCGAGACCA GCCAGGCCGG CCTGTTCAGG
CTGTGGGCGC TCGTTGGCAG TGACTTGCAC TGCATCAGGC TGAGCATCCC CCGTGTGTTC
TACGTGAACC AGCGAGTCGC TAAAGCGGAG GAGGGTGCTT CGTATCGCAA GGTAAATCGG
GTCCTTCCTC GCTCCAACAT GGTCTACAAT CTCTATGAGT ATTCAGTGCC AGAGGACATG
TACCAGGAAC ACATCAACGA GATCAACGCT GAGCTGTCAG CGCCAGACAT CGAGGGCGTA
TATGAGACTC AGGTTCCGTT ACTGTTCCGG GCCCTGGTGC ACCTGGGCTG TGTGTGTGTG
GTCAATAAAC AGCTGGTGAG GCACCTTTCA GGCTGGGAAG CAGAGACCTT TGCTCTTGAG
CACCTGGAGA TGCGCTCTCT GGCCCAGTTC AGCTACCTGG AACCAGGGAG TATCCGCCAT
ATCTACCTGT ACCACCACGC ACAGGCCCAC AAAGCGCTCT TCGGGATCTT CATCCCCTCA
CAGCGCAGGG CATCCGTCTT TGTGCTGGAC ACTGTGCGCA GCAACCAGAT GCCCAGCCTT
GGCGCCCTGT ACTCAGCAGA GCACGGCCTC CTCCTGGAGA AGGTGGGCCC TGAGCTCCTG
CCACCCCCCA AACACACCTT CGAAGTTCGG GCAGAAACTG ACCTGAAGAC CATCTGCAGA
GCCATCCAGC GATTCCTGCT CGCCTACAAG GAGGAGCGCC GGGGGCCCAC ACTCATCGCT
GTTCAGTCCA GCTGGGAGCT GAAGAGGCTG GCCAGTGAAA TTCCTGTCTT GGAGGAATTC
CCACTGGTGC CTATCTGTGT GGCTGACAAG ATCAACTATG GGGTCCTGGA CTGGCAGCGC
CATGGAGCCC GGCGCATGAT CCGTCACTAC CTCAACCTGG ACACCTGCCT GTCGCAGGCC
TTCGAGATGA GCAGGTACTT TCACATTCCC ATTGGGAACC TACCAGAGGA CATCTCCACA
TTCGGCTCCG ACCTCTTCTT TGCCCGCCAC CTCCAGCGCC ACAACCACCT GCTCTGGCTG
TCCCCTACAG CCCGCCCTGA CCTGGGTGGA AAGGAGGCTG ATGACAACTG TCTTGTCATG
GAGTTCGATG ACCAAGCCAC TGTTGAGATC AACAGTTCAG GCTGTTACTC CACAGTGTGT
GTGGAGCTGG ACCTTCAGAA CCTGGCCGTC AACACCATTC TCCAGTCTCA CCATGTCAAC
GACATGGAGG GGGCCGACAG CATGGGGATC AGCTTCGACG TGATCCAGCA GGCCTCCCTG
GAGGACATGA TCACGGGTGG TCAGGCTGCC AGTGCCCCGG CCAGCTACGA TGAGACAGCC
CTGTGCTCTA ACACCTTCAG GATCCTGAAG AGCATGGTCG TGGGCTGGGT GAAGGAGATC
ACCCAGTACC ACAACATCTA TGCAGACAAC CAGGTGATGC ACTTCTACCG CTGGCTTCGG
TCGCCATCCT CTCTGCTTCA TGACCCTGCC CTGCACCGCA CACTCCACAA CATGATGAAG
AAGCTCTTCC TGCAGCTCAT CGCTGAGTTC AAGCGCCTGG GGTCATCAGT CATCTACGCC
AACTTCAACC GCATCATCCT CTGTACAAAG AAGCGCCGTG TGGAAGATGC CATCGCTTAC
GTGGAGTACA TCACCAGCAG CATCCATTCA AAGGAGACCT TCCATTCTCT GACAATTTCT
TTCTCTCGAT GCTGGGAATT TCTTCTCTGG ATGGATCCAT CTAACTATGG CGGAATCAAA
GGAAAAGTTT CATCTCGTAT TCACTGTGGA CTGCAAGACT CCCAGAAAGC AGGGGGAGCA
GAGGATGAGC AGGAAAATGA GGACGATGAG GAGGAAAGAG ATGGGGAGGA GGAGGAAGAG
GCGGAGGAAT CCAACGTGGA GGATTTACTG GAAAACAACT GGAACATTTT GCAGTTTTTG
CCACAGGCAG CCTCCTGCCA GAACTACTTC CTCATGATTG TTTCAGCGTA CATCGTGGCC
GTGTACCACT GCATGAAGGA CGGGCTGAGG CGCAGTGCTC CAGGGAGCAC CCCCGTGAGG
AGGAGGGGGG CCAGCCAGCT CTCCCAGGAG GCCGAGGGGG CGGTCGGAGC CCTTCCCGGA
ATGATCACCT TCTCTCAGGA TTATGTCGCA AATGAGCTCA CTCAGAGCTT CTTCACCATC
ACTCAGAAGA TTCAGAAGAA AGTCACAGGC TCTCGGAACT CCACTGAGCT CTCAGAGATG
TTTCCTGTCC TCCCCGGTTC CCACTTGCTG CTCAATAACC CTGCCCTGGA GTTCATCAAA
TACGTGTGCA AGGTGCTGTC CCTGGACACC AACATCACAA ACCAGGTGAA TAAGCTGAAC
CGAGACCTGC TTCGCCTGGT GGATGTCGGC GAGTTCTCCG AGGAGGCCCA GTTCCGAGAC
CCCTGCCGCT CCTACGTGCT TCCTGAGGTC ATCTGCCGCA GCTGTAACTT CTGCCGCGAC
CTGGACCTGT GTAAAGACTC TTCCTTCTCA GAGGATGGGG CGGTCCTGCC TCAGTGGCTC
TGCTCCAACT GTCAGGCGCC CTACGACTCC TCTGCCATCG AGATGACGCT GGTGGAAGTT
CTACAGAAGA AGCTGATGGC CTTCACCCTG CAGGACCTGC GGAAGGAGGC AGACACTCCC
AAGTGTGAAG AGAAGGAAAG GAGGAAGAGG GAGTTGCACA GCTCGGCCAC AGGAAGGTCT
TGCCGAGGGG CATCTGAGCA GAGGCTGGGG GACGCGCCAA GCTGCCGTGT GGTGGTGCAC
GGCGTTCAGG CCTGCAGGAT GGAGGGAGTG AGCAGGGCCT GGAGGAGGCC TCAGGACTGG
GCATCTGGGG TGGAACATCC ACAGCGAGGG TGGGTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59812
Clone ID Related Accession (Same CDS sequence) XM_011534799
Accession Version XM_011534799.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5436bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA polymerase epsilon catalytic subunit A isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024477.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGG CCACTTTTCG ATGTGACCAA
AGGTACATCA CAGGGATCTC CAGCCAGCTC TACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533101.1
CDS210..5645
Misc Feature(1)504..3704(+)
Misc Feature(2)1011..1622(+)
Misc Feature(3)1032..1595(+)
Misc Feature(4)1032..1595(+)
Misc Feature(5)1035..1595(+)
Misc Feature(6)1800..3668(+)
Misc Feature(7)2085..2795(+)
Misc Feature(8)2787..2798(+)
Misc Feature(9)4782..>5588(+)
Translation

Target ORF information:

RefSeq Version XM_011534799
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011534799

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGG CCACTTTTCG ATGTGACCAA
AGGTACATCA CAGGGATCTC CAGCCAGCTC TACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59813
Clone ID Related Accession (Same CDS sequence) XM_011534800
Accession Version XM_011534800.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5412bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA polymerase epsilon catalytic subunit A isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024477.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGT ACATCACAGG GATCTCCAGC
CAGCTCTACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533102.1
CDS210..5621
Misc Feature(1)504..3704(+)
Misc Feature(2)1011..1622(+)
Misc Feature(3)1032..1595(+)
Misc Feature(4)1032..1595(+)
Misc Feature(5)1035..1595(+)
Misc Feature(6)1800..3668(+)
Misc Feature(7)2085..2795(+)
Misc Feature(8)2787..2798(+)
Misc Feature(9)4782..>5588(+)
Translation

Target ORF information:

RefSeq Version XM_011534800
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011534800

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGT ACATCACAGG GATCTCCAGC
CAGCTCTACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59814
Clone ID Related Accession (Same CDS sequence) XM_011534801
Accession Version XM_011534801.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4338bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA polymerase epsilon catalytic subunit A isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024477.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG
TATCGCAAGG AATTCCTCAA AACAACGTGT GTGAGCGCTG AGCACCGAGC CTGGGGCAGA
GCGAGTGCCG GGAATGGAGC TGCTGGTGTC CTTGTCACGA TGAGCTTGTC TGCCCGCGAG
GTCCATGTTG CGGGGACAAA CCAAGTGGCA TTGGTTAGAG ATTGTGAACT CTGTCTCCGT
AGGCTCTTTG GTTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533103.1
CDS210..4547
Misc Feature(1)504..3704(+)
Misc Feature(2)1011..1622(+)
Misc Feature(3)1032..1595(+)
Misc Feature(4)1032..1595(+)
Misc Feature(5)1035..1595(+)
Misc Feature(6)1800..3668(+)
Misc Feature(7)2085..2795(+)
Misc Feature(8)2787..2798(+)
Translation

Target ORF information:

RefSeq Version XM_011534801
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011534801

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG
TATCGCAAGG AATTCCTCAA AACAACGTGT GTGAGCGCTG AGCACCGAGC CTGGGGCAGA
GCGAGTGCCG GGAATGGAGC TGCTGGTGTC CTTGTCACGA TGAGCTTGTC TGCCCGCGAG
GTCCATGTTG CGGGGACAAA CCAAGTGGCA TTGGTTAGAG ATTGTGAACT CTGTCTCCGT
AGGCTCTTTG GTTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59815
Clone ID Related Accession (Same CDS sequence) XM_011534802
Accession Version XM_011534802.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA polymerase epsilon catalytic subunit A isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024477.15) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGAAGCAAG AAGGGGACCC CGTGCTTCCT GGGCTCCTGG AGACCAAGGC AGCCAACATG 
CCTGACTCTG AGCTATTCGA GCTCATCTCT GAGAACCGTT CCATGTCTCG GAAGCTGGAA
GATTACGGGG AGCAGAAGTC TACGTCCATC AGCACAGCAA AGCGCCTGGC CGAGTTCCTG
GGAGACCAGA TGGTCAAGGA TGCAGGGCTG AGTTGCCGCT ACATCATCTC CCGCAAGCCC
GAGGGCTCCC CTGTCACGGA GAGGGCCATC CCACTTGCCA TTTTCCAAGC AGAGCCCACG
GTGAGGAAGC ACTTTCTCCG GAAATGGCTC AAGAGCTCTT CCCTTCAAGA CTTTGATATT
CGAGCAATTC TGGATTGGGA CTACTACATT GAGCGGCTGG GAAGCGCCAT CCAGAAGATC
ATCACCATCC CTGCGGCCCT GCAGCAGGTA AAGAACCCAG TGCCACGTGT CAAACACCCC
GACTGGCTGC ACAAAAAACT GCTGGAGAAG AATGATGTCT ACAAGCAGAA GAAGATCAGT
GAGCTCTTCA CCCTGGAGGG CAGGAGACAG GTCACGATGG CCGAGGCCTC AGAAGACAGT
CCGAGGCCAA GTGCTCCTGA CATGGAGGAC TTCGGCCTCG TAAAGCTGCC TCACCCAGCA
GCCCCTGTCA CTGTGAAGAG GAAGCGAGTT CTTTGGGAGA GCCAGGAGGA GTCCCAGGAC
CTCACGCCGA CTGTGCCCTG GCAGGAAATC TTGGGGCAGC CTCCCGCCCT GGGAACCAGC
CAGGAGGAAT GGCTTGTCTG GCTCCGGTTC CACAAGAAGA AGTGGCAGCT GCAGGCCCGG
CAGCGCCTCG CCCGCAGGAA GAGGCAGCGT CTGGAGTCGG CAGAGGGTGT GCTCAGGCCC
GGGGCCATCC GGGATGGTCC TGCCACGGGG CTGGGGAGCT TCTTGCGAAG AACTGCCCGC
AGCATCCTGG ACCTTCCGTG GCAGATTGTG CAGATCAGCG AGACCAGCCA GGCCGGCCTG
TTCAGGCTGT GGGCGCTCGT TGGCAGTGAC TTGCACTGCA TCAGGCTGAG CATCCCCCGT
GTGTTCTACG TGAACCAGCG AGTCGCTAAA GCGGAGGAGG GTGCTTCGTA TCGCAAGGTA
AATCGGGTCC TTCCTCGCTC CAACATGGTC TACAATCTCT ATGAGTATTC AGTGCCAGAG
GACATGTACC AGGAACACAT CAACGAGATC AACGCTGAGC TGTCAGCGCC AGACATCGAG
GGCGTATATG AGACTCAGGT TCCGTTACTG TTCCGGGCCC TGGTGCACCT GGGCTGTGTG
TGTGTGGTCA ATAAACAGCT GGTGAGGCAC CTTTCAGGCT GGGAAGCAGA GACCTTTGCT
CTTGAGCACC TGGAGATGCG CTCTCTGGCC CAGTTCAGCT ACCTGGAACC AGGGAGTATC
CGCCATATCT ACCTGTACCA CCACGCACAG GCCCACAAAG CGCTCTTCGG GATCTTCATC
CCCTCACAGC GCAGGGCATC CGTCTTTGTG CTGGACACTG TGCGCAGCAA CCAGATGCCC
AGCCTTGGCG CCCTGTACTC AGCAGAGCAC GGCCTCCTCC TGGAGAAGGT GGGCCCTGAG
CTCCTGCCAC CCCCCAAACA CACCTTCGAA GTTCGGGCAG AAACTGACCT GAAGACCATC
TGCAGAGCCA TCCAGCGATT CCTGCTCGCC TACAAGGAGG AGCGCCGGGG GCCCACACTC
ATCGCTGTTC AGTCCAGCTG GGAGCTGAAG AGGCTGGCCA GTGAAATTCC TGTCTTGGAG
GAATTCCCAC TGGTGCCTAT CTGTGTGGCT GACAAGATCA ACTATGGGGT CCTGGACTGG
CAGCGCCATG GAGCCCGGCG CATGATCCGT CACTACCTCA ACCTGGACAC CTGCCTGTCG
CAGGCCTTCG AGATGAGCAG GTACTTTCAC ATTCCCATTG GGAACCTACC AGAGGACATC
TCCACATTCG GCTCCGACCT CTTCTTTGCC CGCCACCTCC AGCGCCACAA CCACCTGCTC
TGGCTGTCCC CTACAGCCCG CCCTGACCTG GGTGGAAAGG AGGCTGATGA CAACTGTCTT
GTCATGGAGT TCGATGACCA AGCCACTGTT GAGATCAACA GTTCAGGCTG TTACTCCACA
GTGTGTGTGG AGCTGGACCT TCAGAACCTG GCCGTCAACA CCATTCTCCA GTCTCACCAT
GTCAACGACA TGGAGGGGGC CGACAGCATG GGGATCAGCT TCGACGTGAT CCAGCAGGCC
TCCCTGGAGG ACATGATCAC GGGTGGTCAG GCTGCCAGTG CCCCGGCCAG CTACGATGAG
ACAGCCCTGT GCTCTAACAC CTTCAGGATC CTGAAGAGCA TGGTCGTGGG CTGGGTGAAG
GAGATCACCC AGTACCACAA CATCTATGCA GACAACCAGG TGATGCACTT CTACCGCTGG
CTTCGGTCGC CATCCTCTCT GCTTCATGAC CCTGCCCTGC ACCGCACACT CCACAACATG
ATGAAGAAGC TCTTCCTGCA GCTCATCGCT GAGTTCAAGC GCCTGGGGTC ATCAGTCATC
TACGCCAACT TCAACCGCAT CATCCTCTGT ACAAAGAAGC GCCGTGTGGA AGATGCCATC
GCTTACGTGG AGTACATCAC CAGCAGCATC CATTCAAAGG AGACCTTCCA TTCTCTGACA
ATTTCTTTCT CTCGATGCTG GGAATTTCTT CTCTGGATGG ATCCATCTAA CTATGGCGGA
ATCAAAGGAA AAGTTTCATC TCGTATTCAC TGTGGACTGC AAGACTCCCA GAAAGCAGGG
GGAGCAGAGG ATGAGCAGGA AAATGAGGAC GATGAGGAGG AAAGAGATGG GGAGGAGGAG
GAAGAGGCGG AGGAATCCAA CGTGGAGGAT TTACTGGAAA ACAACTGGAA CATTTTGCAG
TTTTTGCCAC AGGCAGCCTC CTGCCAGAAC TACTTCCTCA TGATTGTTTC AGCGTACATC
GTGGCCGTGT ACCACTGCAT GAAGGACGGG CTGAGGCGCA GTGCTCCAGG GAGCACCCCC
GTGAGGAGGA GGGGGGCCAG CCAGCTCTCC CAGGAGGCCG AGGGGGCGGT CGGAGCCCTT
CCCGGAATGA TCACCTTCTC TCAGGATTAT GTCGCAAATG AGCTCACTCA GAGCTTCTTC
ACCATCACTC AGAAGATTCA GAAGAAAGTC ACAGGCTCTC GGAACTCCAC TGAGCTCTCA
GAGATGTTTC CTGTCCTCCC CGGTTCCCAC TTGCTGCTCA ATAACCCTGC CCTGGAGTTC
ATCAAATACG TGTGCAAGGT GCTGTCCCTG GACACCAACA TCACAAACCA GGTGAATAAG
CTGAACCGAG ACCTGCTTCG CCTGGTGGAT GTCGGCGAGT TCTCCGAGGA GGCCCAGTTC
CGAGACCCCT GCCGCTCCTA CGTGCTTCCT GAGGTCATCT GCCGCAGCTG TAACTTCTGC
CGCGACCTGG ACCTGTGTAA AGACTCTTCC TTCTCAGAGG ATGGGGCGGT CCTGCCTCAG
TGGCTCTGCT CCAACTGTCA GGCGCCCTAC GACTCCTCTG CCATCGAGAT GACGCTGGTG
GAAGTTCTAC AGAAGAAGCT GATGGCCTTC ACCCTGCAGG ACCTGCGGAA GGAGGCAGAC
ACTCCCAAGT GTGAAGAGAA GGAAAGGAGG AAGAGGGAGT TGCACAGCTC GGCCACAGGA
AGGTCTTGCC GAGGGGCATC TGAGCAGAGG CTGGGGGACG CGCCAAGCTG CCGTGTGGTG
GTGCACGGCG TTCAGGCCTG CAGGATGGAG GGAGTGAGCA GGGCCTGGAG GAGGCCTCAG
GACTGGGCAT CTGGGGTGGA ACATCCACAG CGAGGGTGGG TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533104.1
CDS39..3923
Misc Feature(1)72..485(+)
Misc Feature(2)1599..2801(+)
Translation

Target ORF information:

RefSeq Version XM_011534802
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011534802

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGAAGCAAG AAGGGGACCC CGTGCTTCCT GGGCTCCTGG AGACCAAGGC AGCCAACATG 
CCTGACTCTG AGCTATTCGA GCTCATCTCT GAGAACCGTT CCATGTCTCG GAAGCTGGAA
GATTACGGGG AGCAGAAGTC TACGTCCATC AGCACAGCAA AGCGCCTGGC CGAGTTCCTG
GGAGACCAGA TGGTCAAGGA TGCAGGGCTG AGTTGCCGCT ACATCATCTC CCGCAAGCCC
GAGGGCTCCC CTGTCACGGA GAGGGCCATC CCACTTGCCA TTTTCCAAGC AGAGCCCACG
GTGAGGAAGC ACTTTCTCCG GAAATGGCTC AAGAGCTCTT CCCTTCAAGA CTTTGATATT
CGAGCAATTC TGGATTGGGA CTACTACATT GAGCGGCTGG GAAGCGCCAT CCAGAAGATC
ATCACCATCC CTGCGGCCCT GCAGCAGGTA AAGAACCCAG TGCCACGTGT CAAACACCCC
GACTGGCTGC ACAAAAAACT GCTGGAGAAG AATGATGTCT ACAAGCAGAA GAAGATCAGT
GAGCTCTTCA CCCTGGAGGG CAGGAGACAG GTCACGATGG CCGAGGCCTC AGAAGACAGT
CCGAGGCCAA GTGCTCCTGA CATGGAGGAC TTCGGCCTCG TAAAGCTGCC TCACCCAGCA
GCCCCTGTCA CTGTGAAGAG GAAGCGAGTT CTTTGGGAGA GCCAGGAGGA GTCCCAGGAC
CTCACGCCGA CTGTGCCCTG GCAGGAAATC TTGGGGCAGC CTCCCGCCCT GGGAACCAGC
CAGGAGGAAT GGCTTGTCTG GCTCCGGTTC CACAAGAAGA AGTGGCAGCT GCAGGCCCGG
CAGCGCCTCG CCCGCAGGAA GAGGCAGCGT CTGGAGTCGG CAGAGGGTGT GCTCAGGCCC
GGGGCCATCC GGGATGGTCC TGCCACGGGG CTGGGGAGCT TCTTGCGAAG AACTGCCCGC
AGCATCCTGG ACCTTCCGTG GCAGATTGTG CAGATCAGCG AGACCAGCCA GGCCGGCCTG
TTCAGGCTGT GGGCGCTCGT TGGCAGTGAC TTGCACTGCA TCAGGCTGAG CATCCCCCGT
GTGTTCTACG TGAACCAGCG AGTCGCTAAA GCGGAGGAGG GTGCTTCGTA TCGCAAGGTA
AATCGGGTCC TTCCTCGCTC CAACATGGTC TACAATCTCT ATGAGTATTC AGTGCCAGAG
GACATGTACC AGGAACACAT CAACGAGATC AACGCTGAGC TGTCAGCGCC AGACATCGAG
GGCGTATATG AGACTCAGGT TCCGTTACTG TTCCGGGCCC TGGTGCACCT GGGCTGTGTG
TGTGTGGTCA ATAAACAGCT GGTGAGGCAC CTTTCAGGCT GGGAAGCAGA GACCTTTGCT
CTTGAGCACC TGGAGATGCG CTCTCTGGCC CAGTTCAGCT ACCTGGAACC AGGGAGTATC
CGCCATATCT ACCTGTACCA CCACGCACAG GCCCACAAAG CGCTCTTCGG GATCTTCATC
CCCTCACAGC GCAGGGCATC CGTCTTTGTG CTGGACACTG TGCGCAGCAA CCAGATGCCC
AGCCTTGGCG CCCTGTACTC AGCAGAGCAC GGCCTCCTCC TGGAGAAGGT GGGCCCTGAG
CTCCTGCCAC CCCCCAAACA CACCTTCGAA GTTCGGGCAG AAACTGACCT GAAGACCATC
TGCAGAGCCA TCCAGCGATT CCTGCTCGCC TACAAGGAGG AGCGCCGGGG GCCCACACTC
ATCGCTGTTC AGTCCAGCTG GGAGCTGAAG AGGCTGGCCA GTGAAATTCC TGTCTTGGAG
GAATTCCCAC TGGTGCCTAT CTGTGTGGCT GACAAGATCA ACTATGGGGT CCTGGACTGG
CAGCGCCATG GAGCCCGGCG CATGATCCGT CACTACCTCA ACCTGGACAC CTGCCTGTCG
CAGGCCTTCG AGATGAGCAG GTACTTTCAC ATTCCCATTG GGAACCTACC AGAGGACATC
TCCACATTCG GCTCCGACCT CTTCTTTGCC CGCCACCTCC AGCGCCACAA CCACCTGCTC
TGGCTGTCCC CTACAGCCCG CCCTGACCTG GGTGGAAAGG AGGCTGATGA CAACTGTCTT
GTCATGGAGT TCGATGACCA AGCCACTGTT GAGATCAACA GTTCAGGCTG TTACTCCACA
GTGTGTGTGG AGCTGGACCT TCAGAACCTG GCCGTCAACA CCATTCTCCA GTCTCACCAT
GTCAACGACA TGGAGGGGGC CGACAGCATG GGGATCAGCT TCGACGTGAT CCAGCAGGCC
TCCCTGGAGG ACATGATCAC GGGTGGTCAG GCTGCCAGTG CCCCGGCCAG CTACGATGAG
ACAGCCCTGT GCTCTAACAC CTTCAGGATC CTGAAGAGCA TGGTCGTGGG CTGGGTGAAG
GAGATCACCC AGTACCACAA CATCTATGCA GACAACCAGG TGATGCACTT CTACCGCTGG
CTTCGGTCGC CATCCTCTCT GCTTCATGAC CCTGCCCTGC ACCGCACACT CCACAACATG
ATGAAGAAGC TCTTCCTGCA GCTCATCGCT GAGTTCAAGC GCCTGGGGTC ATCAGTCATC
TACGCCAACT TCAACCGCAT CATCCTCTGT ACAAAGAAGC GCCGTGTGGA AGATGCCATC
GCTTACGTGG AGTACATCAC CAGCAGCATC CATTCAAAGG AGACCTTCCA TTCTCTGACA
ATTTCTTTCT CTCGATGCTG GGAATTTCTT CTCTGGATGG ATCCATCTAA CTATGGCGGA
ATCAAAGGAA AAGTTTCATC TCGTATTCAC TGTGGACTGC AAGACTCCCA GAAAGCAGGG
GGAGCAGAGG ATGAGCAGGA AAATGAGGAC GATGAGGAGG AAAGAGATGG GGAGGAGGAG
GAAGAGGCGG AGGAATCCAA CGTGGAGGAT TTACTGGAAA ACAACTGGAA CATTTTGCAG
TTTTTGCCAC AGGCAGCCTC CTGCCAGAAC TACTTCCTCA TGATTGTTTC AGCGTACATC
GTGGCCGTGT ACCACTGCAT GAAGGACGGG CTGAGGCGCA GTGCTCCAGG GAGCACCCCC
GTGAGGAGGA GGGGGGCCAG CCAGCTCTCC CAGGAGGCCG AGGGGGCGGT CGGAGCCCTT
CCCGGAATGA TCACCTTCTC TCAGGATTAT GTCGCAAATG AGCTCACTCA GAGCTTCTTC
ACCATCACTC AGAAGATTCA GAAGAAAGTC ACAGGCTCTC GGAACTCCAC TGAGCTCTCA
GAGATGTTTC CTGTCCTCCC CGGTTCCCAC TTGCTGCTCA ATAACCCTGC CCTGGAGTTC
ATCAAATACG TGTGCAAGGT GCTGTCCCTG GACACCAACA TCACAAACCA GGTGAATAAG
CTGAACCGAG ACCTGCTTCG CCTGGTGGAT GTCGGCGAGT TCTCCGAGGA GGCCCAGTTC
CGAGACCCCT GCCGCTCCTA CGTGCTTCCT GAGGTCATCT GCCGCAGCTG TAACTTCTGC
CGCGACCTGG ACCTGTGTAA AGACTCTTCC TTCTCAGAGG ATGGGGCGGT CCTGCCTCAG
TGGCTCTGCT CCAACTGTCA GGCGCCCTAC GACTCCTCTG CCATCGAGAT GACGCTGGTG
GAAGTTCTAC AGAAGAAGCT GATGGCCTTC ACCCTGCAGG ACCTGCGGAA GGAGGCAGAC
ACTCCCAAGT GTGAAGAGAA GGAAAGGAGG AAGAGGGAGT TGCACAGCTC GGCCACAGGA
AGGTCTTGCC GAGGGGCATC TGAGCAGAGG CTGGGGGACG CGCCAAGCTG CCGTGTGGTG
GTGCACGGCG TTCAGGCCTG CAGGATGGAG GGAGTGAGCA GGGCCTGGAG GAGGCCTCAG
GACTGGGCAT CTGGGGTGGA ACATCCACAG CGAGGGTGGG TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20900
Clone ID Related Accession (Same CDS sequence) NM_006231
Accession Version NM_006231.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6861bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product DNA polymerase epsilon catalytic subunit A
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC135586.8, L09561.1, CX782956.1 and BC011376.1. This sequence is a reference standard in the RefSeqGene project. On Sep 26, 2013 this sequence version replaced gi:62198236. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L09561.1, U49356.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGA TCCTGAAGAG CATGGTCGTG
GGCTGGGTGA AGGAGATCAC CCAGTACCAC AACATCTATG CAGACAACCA GGTGATGCAC
TTCTACCGCT GGCTTCGGTC GCCATCCTCT CTGCTTCATG ACCCTGCCCT GCACCGCACA
CTCCACAACA TGATGAAGAA GCTCTTCCTG CAGCTCATCG CTGAGTTCAA GCGCCTGGGG
TCATCAGTCA TCTACGCCAA CTTCAACCGC ATCATCCTCT GTACAAAGAA GCGCCGTGTG
GAAGATGCCA TCGCTTACGT GGAGTACATC ACCAGCAGCA TCCATTCAAA GGAGACCTTC
CATTCTCTGA CAATTTCTTT CTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCATCT
AACTATGGCG GAATCAAAGG AAAAGTTTCA TCTCGTATTC ACTGTGGACT GCAAGACTCC
CAGAAAGCAG GGGGAGCAGA GGATGAGCAG GAAAATGAGG ACGATGAGGA GGAAAGAGAT
GGGGAGGAGG AGGAAGAGGC GGAGGAATCC AACGTGGAGG ATTTACTGGA AAACAACTGG
AACATTTTGC AGTTTTTGCC ACAGGCAGCC TCCTGCCAGA ACTACTTCCT CATGATTGTT
TCAGCGTACA TCGTGGCCGT GTACCACTGC ATGAAGGACG GGCTGAGGCG CAGTGCTCCA
GGGAGCACCC CCGTGAGGAG GAGGGGGGCC AGCCAGCTCT CCCAGGAGGC CGAGGGGGCG
GTCGGAGCCC TTCCCGGAAT GATCACCTTC TCTCAGGATT ATGTCGCAAA TGAGCTCACT
CAGAGCTTCT TCACCATCAC TCAGAAGATT CAGAAGAAAG TCACAGGCTC TCGGAACTCC
ACTGAGCTCT CAGAGATGTT TCCTGTCCTC CCCGGTTCCC ACTTGCTGCT CAATAACCCT
GCCCTGGAGT TCATCAAATA CGTGTGCAAG GTGCTGTCCC TGGACACCAA CATCACAAAC
CAGGTGAATA AGCTGAACCG AGACCTGCTT CGCCTGGTGG ATGTCGGCGA GTTCTCCGAG
GAGGCCCAGT TCCGAGACCC CTGCCGCTCC TACGTGCTTC CTGAGGTCAT CTGCCGCAGC
TGTAACTTCT GCCGCGACCT GGACCTGTGT AAAGACTCTT CCTTCTCAGA GGATGGGGCG
GTCCTGCCTC AGTGGCTCTG CTCCAACTGT CAGGCGCCCT ACGACTCCTC TGCCATCGAG
ATGACGCTGG TGGAAGTTCT ACAGAAGAAG CTGATGGCCT TCACCCTGCA GGACCTGGTC
TGCCTGAAGT GCCGCGGGGT GAAGGAGACC AGCATGCCTG TGTACTGCAG CTGCGCGGGA
GACTTCGCCC TCACCATCCA CACCCAGGTC TTCATGGAAC AGATCGGAAT ATTCCGGAAC
ATTGCCCAGC ACTACGGCAT GTCGTACCTC CTGGAGACCC TGGAGTGGCT GCTGCAGAAG
AACCCACAGC TGGGCCATTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006222.2
CDS210..7070
Misc Feature(1)120..122(+)
Misc Feature(2)504..3704(+)
Misc Feature(3)1011..1622(+)
Misc Feature(4)1032..1595(+)
Misc Feature(5)1032..1595(+)
Misc Feature(6)1035..1595(+)
Misc Feature(7)1800..3668(+)
Misc Feature(8)2085..2795(+)
Misc Feature(9)2787..2798(+)
Misc Feature(10)3759..3761(+)
Misc Feature(11)4782..5984(+)
Misc Feature(12)6027..6029(+)
Misc Feature(13)6027..6029(+)
Misc Feature(14)6870..6923(+)
Exon (1)1..271
Gene:POLE
Gene Synonym:
Exon (2)272..413
Gene:POLE
Gene Synonym:
Exon (3)414..494
Gene:POLE
Gene Synonym:
Exon (4)495..539
Gene:POLE
Gene Synonym:
Exon (5)540..632
Gene:POLE
Gene Synonym:
Exon (6)633..787
Gene:POLE
Gene Synonym:
Exon (7)788..929
Gene:POLE
Gene Synonym:
Exon (8)930..1010
Gene:POLE
Gene Synonym:
Exon (9)1011..1118
Gene:POLE
Gene Synonym:
Exon (10)1119..1229
Gene:POLE
Gene Synonym:
Exon (11)1230..1315
Gene:POLE
Gene Synonym:
Exon (12)1316..1435
Gene:POLE
Gene Synonym:
Exon (13)1436..1568
Gene:POLE
Gene Synonym:
Exon (14)1569..1682
Gene:POLE
Gene Synonym:
Exon (15)1683..1895
Gene:POLE
Gene Synonym:
Exon (16)1896..2003
Gene:POLE
Gene Synonym:
Exon (17)2004..2132
Gene:POLE
Gene Synonym:
Exon (18)2133..2235
Gene:POLE
Gene Synonym:
Exon (19)2236..2382
Gene:POLE
Gene Synonym:
Exon (20)2383..2528
Gene:POLE
Gene Synonym:
Exon (21)2529..2677
Gene:POLE
Gene Synonym:
Exon (22)2678..2770
Gene:POLE
Gene Synonym:
Exon (23)2771..2915
Gene:POLE
Gene Synonym:
Exon (24)2916..3073
Gene:POLE
Gene Synonym:
Exon (25)3074..3269
Gene:POLE
Gene Synonym:
Exon (26)3270..3484
Gene:POLE
Gene Synonym:
Exon (27)3485..3587
Gene:POLE
Gene Synonym:
Exon (28)3588..3668
Gene:POLE
Gene Synonym:
Exon (29)3669..3791
Gene:POLE
Gene Synonym:
Exon (30)3792..4004
Gene:POLE
Gene Synonym:
Exon (31)4005..4214
Gene:POLE
Gene Synonym:
Exon (32)4215..4358
Gene:POLE
Gene Synonym:
Exon (33)4359..4499
Gene:POLE
Gene Synonym:
Exon (34)4500..4653
Gene:POLE
Gene Synonym:
Exon (35)4654..4760
Gene:POLE
Gene Synonym:
Exon (36)4761..4937
Gene:POLE
Gene Synonym:
Exon (37)4938..5161
Gene:POLE
Gene Synonym:
Exon (38)5162..5382
Gene:POLE
Gene Synonym:
Exon (39)5383..5587
Gene:POLE
Gene Synonym:
Exon (40)5588..5761
Gene:POLE
Gene Synonym:
Exon (41)5762..5887
Gene:POLE
Gene Synonym:
Exon (42)5888..6020
Gene:POLE
Gene Synonym:
Exon (43)6021..6213
Gene:POLE
Gene Synonym:
Exon (44)6214..6345
Gene:POLE
Gene Synonym:
Exon (45)6346..6539
Gene:POLE
Gene Synonym:
Exon (46)6540..6740
Gene:POLE
Gene Synonym:
Exon (47)6741..6866
Gene:POLE
Gene Synonym:
Exon (48)6867..6956
Gene:POLE
Gene Synonym:
Exon (49)6957..8005
Gene:POLE
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_006231
Organism Homo sapiens (human)
Definition Homo sapiens polymerase (DNA directed), epsilon, catalytic subunit (POLE), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006231

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGA TCCTGAAGAG CATGGTCGTG
GGCTGGGTGA AGGAGATCAC CCAGTACCAC AACATCTATG CAGACAACCA GGTGATGCAC
TTCTACCGCT GGCTTCGGTC GCCATCCTCT CTGCTTCATG ACCCTGCCCT GCACCGCACA
CTCCACAACA TGATGAAGAA GCTCTTCCTG CAGCTCATCG CTGAGTTCAA GCGCCTGGGG
TCATCAGTCA TCTACGCCAA CTTCAACCGC ATCATCCTCT GTACAAAGAA GCGCCGTGTG
GAAGATGCCA TCGCTTACGT GGAGTACATC ACCAGCAGCA TCCATTCAAA GGAGACCTTC
CATTCTCTGA CAATTTCTTT CTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCATCT
AACTATGGCG GAATCAAAGG AAAAGTTTCA TCTCGTATTC ACTGTGGACT GCAAGACTCC
CAGAAAGCAG GGGGAGCAGA GGATGAGCAG GAAAATGAGG ACGATGAGGA GGAAAGAGAT
GGGGAGGAGG AGGAAGAGGC GGAGGAATCC AACGTGGAGG ATTTACTGGA AAACAACTGG
AACATTTTGC AGTTTTTGCC ACAGGCAGCC TCCTGCCAGA ACTACTTCCT CATGATTGTT
TCAGCGTACA TCGTGGCCGT GTACCACTGC ATGAAGGACG GGCTGAGGCG CAGTGCTCCA
GGGAGCACCC CCGTGAGGAG GAGGGGGGCC AGCCAGCTCT CCCAGGAGGC CGAGGGGGCG
GTCGGAGCCC TTCCCGGAAT GATCACCTTC TCTCAGGATT ATGTCGCAAA TGAGCTCACT
CAGAGCTTCT TCACCATCAC TCAGAAGATT CAGAAGAAAG TCACAGGCTC TCGGAACTCC
ACTGAGCTCT CAGAGATGTT TCCTGTCCTC CCCGGTTCCC ACTTGCTGCT CAATAACCCT
GCCCTGGAGT TCATCAAATA CGTGTGCAAG GTGCTGTCCC TGGACACCAA CATCACAAAC
CAGGTGAATA AGCTGAACCG AGACCTGCTT CGCCTGGTGG ATGTCGGCGA GTTCTCCGAG
GAGGCCCAGT TCCGAGACCC CTGCCGCTCC TACGTGCTTC CTGAGGTCAT CTGCCGCAGC
TGTAACTTCT GCCGCGACCT GGACCTGTGT AAAGACTCTT CCTTCTCAGA GGATGGGGCG
GTCCTGCCTC AGTGGCTCTG CTCCAACTGT CAGGCGCCCT ACGACTCCTC TGCCATCGAG
ATGACGCTGG TGGAAGTTCT ACAGAAGAAG CTGATGGCCT TCACCCTGCA GGACCTGGTC
TGCCTGAAGT GCCGCGGGGT GAAGGAGACC AGCATGCCTG TGTACTGCAG CTGCGCGGGA
GACTTCGCCC TCACCATCCA CACCCAGGTC TTCATGGAAC AGATCGGAAT ATTCCGGAAC
ATTGCCCAGC ACTACGGCAT GTCGTACCTC CTGGAGACCC TGGAGTGGCT GCTGCAGAAG
AACCCACAGC TGGGCCATTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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