||ORF Nucleotide Sequence (Length: 2043bp)
||pcDNA3.1-C-(k)DYK or customized vector
|Tag on pcDNA3.1-C-(k)DYK
||C terminal DYKDDDDK tags
|ORF Insert Method
||CloneEZ® Seamless cloning technology
||Homo sapiens (human)
||NADPH--cytochrome P450 reductase
||REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from DA701694.1, BP244929.1,
BC034277.1, AF258341.1, BG104767.1 and AK129978.1.
This sequence is a reference standard in the RefSeqGene project.
On or before Aug 31, 2013 this sequence version replaced
gi:530386140, gi:530432544, gi:24307876.
Summary: This gene encodes an endoplasmic reticulum membrane
oxidoreductase with an FAD-binding domain and a flavodoxin-like
domain. The protein binds two cofactors, FAD and FMN, which allow
it to donate electrons directly from NADPH to all microsomal P450
enzymes. Mutations in this gene have been associated with various
diseases, including apparent combined P450C17 and P450C21
deficiency, amenorrhea and disordered steroidogenesis, congenital
adrenal hyperplasia and Antley-Bixler syndrome. [provided by
RefSeq, Jul 2008].
CCDS Note: This CCDS representation uses the 5'-most in-frame start
codon, which is conserved in higher primate species. This starting
position is most commonly referred to in the literature, and the
numbering system used to describe disease-associated mutations is
based on this protein start. This includes data in the P450
oxidoreductase (POR) allele nomenclature locus-specific database,
and the Human Gene Mutation Database (HGMD). This start codon is
restricted to higher primate species, and it has a weak Kozak
signal. However, it should be noted that an alternative downstream
start codon, which has a strong Kozak signal and is much more
widely conserved, is also present. The use of this downstream start
codon would result in a protein that is 3 aa shorter at the
N-terminus. Protein sequencing in PMID:2513880 indicates that this
is the preferred start codon in vivo. It is therefore possible that
the ribosome will initiate at the upstream start codon only a small
fraction of the time (if at all), while leaky scanning will allow
the downstream start codon to be used predominantly.
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
Transcript exon combination :: AK290529.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns
COMPLETENESS: complete on the 3' end.