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POU1F1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol POU1F1
Entrez Gene ID 5449
Full Name POU class 1 homeobox 1
Synonyms CPHD1, GHF-1, PIT1, POU1F1a, Pit-1
General protein information
Preferred Names
pituitary-specific positive transcription factor 1
Names
pituitary-specific positive transcription factor 1
growth hormone factor 1
pituitary-specific transcription factor 1
POU domain, class 1, transcription factor 1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

3

3p11

Summary This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

173110

Disorder Html: Pituitary hormone deficiency, combined, 1, 613038 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000306 NP_000297 pituitary-specific positive transcription factor 1 isoform alpha
NM_001122757 NP_001116229 pituitary-specific positive transcription factor 1 isoform beta



Homo sapiens (human) POU1F1 NP_001116229.1
Pan troglodytes (chimpanzee) POU1F1 XP_003318250.1
Macaca mulatta (Rhesus monkey) POU1F1 NP_001036325.1
Canis lupus familiaris (dog) POU1F1 NP_001006950.1
Bos taurus (cattle) POU1F1 NP_777004.1
Mus musculus (house mouse) Pou1f1 NP_032875.1
Rattus norvegicus (Norway rat) Pou1f1 NP_037140.2
Gallus gallus (chicken) POU1F1 NP_989650.1
Danio rerio (zebrafish) pou1f1 NP_998016.1
Xenopus (Silurana) tropicalis (western clawed frog) pou1f1 XP_002931806.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POU1F1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POU1F1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu23957 NM_000306 Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant alpha, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $216.30
$309.00
OHu23455 NM_001122757 Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant beta, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $216.30
$309.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu23957
Clone ID Related Accession (Same CDS sequence) NM_000306
Accession Version NM_000306.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 876bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pituitary-specific positive transcription factor 1 isoform alpha
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D10216.1 and KF457648.1. On Jan 17, 2014 this sequence version replaced gi:170650728. Transcript Variant: This variant (alpha) uses an alternate in-frame splice site in the 5' coding region, compared to variant beta, resulting in a shorter protein (isoform alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D10216.1, X62429.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2158188, SAMEA2162895 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGAGTTGCC AAGCTTTTAC TTCGGCTGAT ACCTTTATAC CTCTGAATTC TGACGCCTCT 
GCAACTCTGC CTCTGATAAT GCATCACAGT GCTGCCGAGT GTCTACCAGT CTCCAACCAT
GCCACCAATG TGATGTCTAC AGCAACAGGA CTTCATTATT CTGTTCCTTC CTGTCATTAT
GGAAACCAGC CATCAACCTA TGGAGTGATG GCAGGTAGTT TAACCCCTTG TCTTTATAAA
TTTCCTGACC ACACCTTGAG TCATGGATTT CCTCCTATAC ACCAGCCTCT TCTGGCAGAG
GACCCCACAG CTGCTGATTT CAAGCAGGAA CTCAGGCGGA AAAGTAAATT GGTGGAAGAG
CCAATAGACA TGGATTCTCC AGAAATCAGA GAACTTGAAA AGTTTGCCAA TGAATTTAAA
GTGAGACGAA TTAAATTAGG ATACACCCAG ACAAATGTTG GGGAGGCCCT GGCAGCTGTG
CATGGCTCTG AATTCAGTCA AACAACAATC TGCCGATTTG AAAATCTGCA GCTCAGCTTT
AAAAATGCAT GCAAACTGAA AGCAATATTA TCCAAATGGC TGGAGGAAGC TGAGCAAGTA
GGAGCTTTGT ACAATGAAAA AGTGGGAGCA AATGAAAGGA AAAGAAAACG AAGAACAACT
ATAAGCATTG CTGCTAAAGA TGCTCTGGAG AGACACTTTG GAGAACAGAA TAAACCTTCT
TCTCAAGAGA TCATGAGGAT GGCTGAAGAA CTGAATCTGG AGAAAGAAGT AGTAAGAGTT
TGGTTTTGCA ACCGGAGGCA GAGAGAAAAA CGGGTGAAAA CAAGTCTGAA TCAGAGTTTA
TTTTCTATTT CTAAGGAACA TCTTGAGTGC AGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000297.1
CDS126..1001
Misc Feature(1)78..80(+)
Misc Feature(2)495..719(+)
Misc Feature(3)768..944(+)
Misc Feature(4)768..938(+)
Misc Feature(5)774..926(+)
Exon (1)1..267
Gene:POU1F1
Gene Synonym:
Exon (2)268..339
Gene:POU1F1
Gene Synonym:
Exon (3)340..564
Gene:POU1F1
Gene Synonym:
Exon (4)565..729
Gene:POU1F1
Gene Synonym:
Exon (5)730..790
Gene:POU1F1
Gene Synonym:
Exon (6)791..1262
Gene:POU1F1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000306
Organism Homo sapiens (human)
Definition Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant alpha, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000306

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGAGTTGCC AAGCTTTTAC TTCGGCTGAT ACCTTTATAC CTCTGAATTC TGACGCCTCT 
GCAACTCTGC CTCTGATAAT GCATCACAGT GCTGCCGAGT GTCTACCAGT CTCCAACCAT
GCCACCAATG TGATGTCTAC AGCAACAGGA CTTCATTATT CTGTTCCTTC CTGTCATTAT
GGAAACCAGC CATCAACCTA TGGAGTGATG GCAGGTAGTT TAACCCCTTG TCTTTATAAA
TTTCCTGACC ACACCTTGAG TCATGGATTT CCTCCTATAC ACCAGCCTCT TCTGGCAGAG
GACCCCACAG CTGCTGATTT CAAGCAGGAA CTCAGGCGGA AAAGTAAATT GGTGGAAGAG
CCAATAGACA TGGATTCTCC AGAAATCAGA GAACTTGAAA AGTTTGCCAA TGAATTTAAA
GTGAGACGAA TTAAATTAGG ATACACCCAG ACAAATGTTG GGGAGGCCCT GGCAGCTGTG
CATGGCTCTG AATTCAGTCA AACAACAATC TGCCGATTTG AAAATCTGCA GCTCAGCTTT
AAAAATGCAT GCAAACTGAA AGCAATATTA TCCAAATGGC TGGAGGAAGC TGAGCAAGTA
GGAGCTTTGT ACAATGAAAA AGTGGGAGCA AATGAAAGGA AAAGAAAACG AAGAACAACT
ATAAGCATTG CTGCTAAAGA TGCTCTGGAG AGACACTTTG GAGAACAGAA TAAACCTTCT
TCTCAAGAGA TCATGAGGAT GGCTGAAGAA CTGAATCTGG AGAAAGAAGT AGTAAGAGTT
TGGTTTTGCA ACCGGAGGCA GAGAGAAAAA CGGGTGAAAA CAAGTCTGAA TCAGAGTTTA
TTTTCTATTT CTAAGGAACA TCTTGAGTGC AGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23455
Clone ID Related Accession (Same CDS sequence) NM_001122757
Accession Version NM_001122757.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pituitary-specific positive transcription factor 1 isoform beta
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC130885.3 and KF457648.1. This sequence is a reference standard in the RefSeqGene project. On Jan 17, 2014 this sequence version replaced gi:170650729. Transcript Variant: This variant (beta), also known as GHF-2 or PIT-2, represents the longer transcript and encodes the longer isoform (beta). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2158188, SAMEA2162895 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAGTTGCC AAGCTTTTAC TTCGGCTGAT ACCTTTATAC CTCTGAATTC TGACGCCTCT 
GCAACTCTGC CTCTGATAAT GCATCACAGT GCTGCCGAGT GTCTACCAGT CTCCAACCAT
GCCACCAATG TGATGTCTAC AGTCCCATCT ATTTTGTCTT TGATCCAAAC TCCTAAATGT
TTGTGCACAC ATTTCTCGGT GACAACGTTG GGAAACACAG CAACAGGACT TCATTATTCT
GTTCCTTCCT GTCATTATGG AAACCAGCCA TCAACCTATG GAGTGATGGC AGGTAGTTTA
ACCCCTTGTC TTTATAAATT TCCTGACCAC ACCTTGAGTC ATGGATTTCC TCCTATACAC
CAGCCTCTTC TGGCAGAGGA CCCCACAGCT GCTGATTTCA AGCAGGAACT CAGGCGGAAA
AGTAAATTGG TGGAAGAGCC AATAGACATG GATTCTCCAG AAATCAGAGA ACTTGAAAAG
TTTGCCAATG AATTTAAAGT GAGACGAATT AAATTAGGAT ACACCCAGAC AAATGTTGGG
GAGGCCCTGG CAGCTGTGCA TGGCTCTGAA TTCAGTCAAA CAACAATCTG CCGATTTGAA
AATCTGCAGC TCAGCTTTAA AAATGCATGC AAACTGAAAG CAATATTATC CAAATGGCTG
GAGGAAGCTG AGCAAGTAGG AGCTTTGTAC AATGAAAAAG TGGGAGCAAA TGAAAGGAAA
AGAAAACGAA GAACAACTAT AAGCATTGCT GCTAAAGATG CTCTGGAGAG ACACTTTGGA
GAACAGAATA AACCTTCTTC TCAAGAGATC ATGAGGATGG CTGAAGAACT GAATCTGGAG
AAAGAAGTAG TAAGAGTTTG GTTTTGCAAC CGGAGGCAGA GAGAAAAACG GGTGAAAACA
AGTCTGAATC AGAGTTTATT TTCTATTTCT AAGGAACATC TTGAGTGCAG ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001116229.1
CDS126..1079
Misc Feature(1)78..80(+)
Misc Feature(2)573..797(+)
Misc Feature(3)846..1022(+)
Misc Feature(4)846..1016(+)
Misc Feature(5)852..1004(+)
Exon (1)1..267
Gene:POU1F1
Gene Synonym:
Exon (2)268..417
Gene:POU1F1
Gene Synonym:
Exon (3)418..642
Gene:POU1F1
Gene Synonym:
Exon (4)643..807
Gene:POU1F1
Gene Synonym:
Exon (5)808..868
Gene:POU1F1
Gene Synonym:
Exon (6)869..1340
Gene:POU1F1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001122757
Organism Homo sapiens (human)
Definition Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant beta, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001122757

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAGTTGCC AAGCTTTTAC TTCGGCTGAT ACCTTTATAC CTCTGAATTC TGACGCCTCT 
GCAACTCTGC CTCTGATAAT GCATCACAGT GCTGCCGAGT GTCTACCAGT CTCCAACCAT
GCCACCAATG TGATGTCTAC AGTCCCATCT ATTTTGTCTT TGATCCAAAC TCCTAAATGT
TTGTGCACAC ATTTCTCGGT GACAACGTTG GGAAACACAG CAACAGGACT TCATTATTCT
GTTCCTTCCT GTCATTATGG AAACCAGCCA TCAACCTATG GAGTGATGGC AGGTAGTTTA
ACCCCTTGTC TTTATAAATT TCCTGACCAC ACCTTGAGTC ATGGATTTCC TCCTATACAC
CAGCCTCTTC TGGCAGAGGA CCCCACAGCT GCTGATTTCA AGCAGGAACT CAGGCGGAAA
AGTAAATTGG TGGAAGAGCC AATAGACATG GATTCTCCAG AAATCAGAGA ACTTGAAAAG
TTTGCCAATG AATTTAAAGT GAGACGAATT AAATTAGGAT ACACCCAGAC AAATGTTGGG
GAGGCCCTGG CAGCTGTGCA TGGCTCTGAA TTCAGTCAAA CAACAATCTG CCGATTTGAA
AATCTGCAGC TCAGCTTTAA AAATGCATGC AAACTGAAAG CAATATTATC CAAATGGCTG
GAGGAAGCTG AGCAAGTAGG AGCTTTGTAC AATGAAAAAG TGGGAGCAAA TGAAAGGAAA
AGAAAACGAA GAACAACTAT AAGCATTGCT GCTAAAGATG CTCTGGAGAG ACACTTTGGA
GAACAGAATA AACCTTCTTC TCAAGAGATC ATGAGGATGG CTGAAGAACT GAATCTGGAG
AAAGAAGTAG TAAGAGTTTG GTTTTGCAAC CGGAGGCAGA GAGAAAAACG GGTGAAAACA
AGTCTGAATC AGAGTTTATT TTCTATTTCT AAGGAACATC TTGAGTGCAG ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Research resource: A genome-wide study identifies potential new target genes for POU1F1
Mol. Endocrinol. 26 (8), 1455-1463 (2012)
Herman JP, Jullien N, Guillen S, Enjalbert A, Pellegrini I and Franc JL.


book

PIT1 upregulation by HMGA proteins has a role in pituitary tumorigenesis
Endocr. Relat. Cancer 19 (2), 123-135 (2012)
Palmieri D, Valentino T, De Martino I, Esposito F, Cappabianca P, Wierinckx A, Vitiello M, Lombardi G, Colao A, Trouillas J, Pierantoni GM, Fusco A and Fedele M.


book

POU1F1-mediated activation of hGH-N by deoxyribonuclease I hypersensitive site II of the human growth hormone locus control region
J. Mol. Biol. 415 (1), 29-45 (2012)
Hunsaker TL, Jefferson HS, Morrison JK, Franklin AJ and Shewchuk BM.


book

Identification of a novel mutation in the exon 2 splice donor site of the POU1F1/PIT-1 gene in Japanese identical twins with mild combined pituitary hormone deficiency
Clin. Endocrinol. (Oxf) 76 (1), 78-87 (2012)
Inoue H, Mukai T, Sakamoto Y, Kimura C, Kangawa N, Itakura M, Ogata T, Ito Y and Fujieda K.


book

CTNNB1 gene mutations, pituitary transcription factors, and MicroRNA expression involvement in the pathogenesis of adamantinomatous craniopharyngiomas
Horm Cancer 1 (4), 187-196 (2010)
Campanini ML, Colli LM, Paixao BM, Cabral TP, Amaral FC, Machado HR, Neder LS, Saggioro F, Moreira AC, Antonini SR and de Castro M.


book

Characterization of the gene encoding human pituitary-specific transcription factor, Pit-1
Gene 122 (2), 387-388 (1992)
Ohta K, Nobukuni Y, Mitsubuchi H, Ohta T, Tohma T, Jinno Y, Endo F and Matsuda I.


book

Mutations in the Pit-1 gene in children with combined pituitary hormone deficiency
Biochem. Biophys. Res. Commun. 189 (2), 851-855 (1992)
Ohta K, Nobukuni Y, Mitsubuchi H, Fujimoto S, Matsuo N, Inagaki H, Endo F and Matsuda I.


book

A mutation in the POU-homeodomain of Pit-1 responsible for combined pituitary hormone deficiency
Science 257 (5073), 1115-1118 (1992)
Radovick S, Nations M, Du Y, Berg LA, Weintraub BD and Wondisford FE.


book

Cretinism with combined hormone deficiency caused by a mutation in the PIT1 gene
Nat. Genet. 1 (1), 56-58 (1992)
Tatsumi K, Miyai K, Notomi T, Kaibe K, Amino N, Mizuno Y and Kohno H.


book

Nucleotide sequence of the complementary DNA for human Pit-1/GHF-1
Biochim. Biophys. Acta 1129 (2), 231-234 (1992)
Tatsumi K, Notomi T, Amino N and Miyai K.


 
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