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POU1F1 POU class 1 homeobox 1 [Homo sapiens (human)]

Gene Symbol POU1F1
Entrez Gene ID 5449
Full Name POU class 1 homeobox 1
Synonyms CPHD1, GHF-1, PIT1, POU1F1a, Pit-1
General protein information
Preferred Names
pituitary-specific positive transcription factor 1
Names
pituitary-specific positive transcription factor 1
growth hormone factor 1
pituitary-specific transcription factor 1
POU domain, class 1, transcription factor 1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

3

3p11

Summary This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

173110

Disorder Html: Pituitary hormone deficiency, combined, 1, 613038 (3)





The following POU1F1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POU1F1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu23957 NM_000306 Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant alpha, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $309
OHu23455 NM_001122757 Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant beta, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $309

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu23957
Accession Version NM_000306.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 876bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pituitary-specific positive transcription factor 1 isoform alpha
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D10216.1 and KF457648.1. On Jan 17, 2014 this sequence version replaced gi:170650728. Summary: This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (alpha) uses an alternate in-frame splice site in the 5' coding region, compared to variant beta, resulting in a shorter protein (isoform alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D10216.1, X62429.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2158188, SAMEA2162895 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGAGTTGCC AAGCTTTTAC TTCGGCTGAT ACCTTTATAC CTCTGAATTC TGACGCCTCT 
GCAACTCTGC CTCTGATAAT GCATCACAGT GCTGCCGAGT GTCTACCAGT CTCCAACCAT
GCCACCAATG TGATGTCTAC AGCAACAGGA CTTCATTATT CTGTTCCTTC CTGTCATTAT
GGAAACCAGC CATCAACCTA TGGAGTGATG GCAGGTAGTT TAACCCCTTG TCTTTATAAA
TTTCCTGACC ACACCTTGAG TCATGGATTT CCTCCTATAC ACCAGCCTCT TCTGGCAGAG
GACCCCACAG CTGCTGATTT CAAGCAGGAA CTCAGGCGGA AAAGTAAATT GGTGGAAGAG
CCAATAGACA TGGATTCTCC AGAAATCAGA GAACTTGAAA AGTTTGCCAA TGAATTTAAA
GTGAGACGAA TTAAATTAGG ATACACCCAG ACAAATGTTG GGGAGGCCCT GGCAGCTGTG
CATGGCTCTG AATTCAGTCA AACAACAATC TGCCGATTTG AAAATCTGCA GCTCAGCTTT
AAAAATGCAT GCAAACTGAA AGCAATATTA TCCAAATGGC TGGAGGAAGC TGAGCAAGTA
GGAGCTTTGT ACAATGAAAA AGTGGGAGCA AATGAAAGGA AAAGAAAACG AAGAACAACT
ATAAGCATTG CTGCTAAAGA TGCTCTGGAG AGACACTTTG GAGAACAGAA TAAACCTTCT
TCTCAAGAGA TCATGAGGAT GGCTGAAGAA CTGAATCTGG AGAAAGAAGT AGTAAGAGTT
TGGTTTTGCA ACCGGAGGCA GAGAGAAAAA CGGGTGAAAA CAAGTCTGAA TCAGAGTTTA
TTTTCTATTT CTAAGGAACA TCTTGAGTGC AGATAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000297.1
CDS126..1001
Misc Feature(1)78..80(+)
Misc Feature(2)495..719(+)
Misc Feature(3)768..944(+)
Misc Feature(4)768..938(+)
Misc Feature(5)774..926(+)
Exon (1)1..267
Gene:POU1F1
Gene Synonym:
Exon (2)268..339
Gene:POU1F1
Gene Synonym:
Exon (3)340..564
Gene:POU1F1
Gene Synonym:
Exon (4)565..729
Gene:POU1F1
Gene Synonym:
Exon (5)730..790
Gene:POU1F1
Gene Synonym:
Exon (6)791..1262
Gene:POU1F1
Gene Synonym:
Translation
Position Chain Variation Link
40 40 c, t dbSNP:547104320
78 78 c, t dbSNP:771498395
84 84 a, g dbSNP:369089154
88 88 a, g dbSNP:778499770
90 90 c, t dbSNP:754597573
93 93 c, g dbSNP:775811831
94 94 a, g dbSNP:372288270
98 98 c, t dbSNP:756681562
100 100 c, t dbSNP:779842896
102 102 c, t dbSNP:755859410
113 113 c, t dbSNP:368528397
115 115 c, g dbSNP:767428065
120 120 g, t dbSNP:762999659
128 128 c, g dbSNP:752815164
131 131 c, t dbSNP:201156427
133 133 a, g dbSNP:759809037
136 136 c, t dbSNP:1051612
139 139 -, c dbSNP:767075581
139 139 c, t dbSNP:776954429
152 152 c, t dbSNP:35560664
156 156 a, t dbSNP:4988459
157 157 a, c, t dbSNP:773651922
164 164 a, g dbSNP:772631185
165 165 c, g dbSNP:200873915
166 166 c, t dbSNP:773832905
181 181 c, t dbSNP:35182189
188 188 a, c dbSNP:146118849
196 196 c, t dbSNP:104893757
197 197 g, t dbSNP:779611176
206 206 a, g dbSNP:142491010
209 209 a, t dbSNP:755914583
213 213 a, c dbSNP:745659872
215 215 a, c, t dbSNP:543096490
222 222 a, g dbSNP:752767611
225 225 c, t dbSNP:368440919
227 227 c, t dbSNP:374097539
229 229 c, t dbSNP:558209279
242 242 a, c, t dbSNP:370682988
245 245 c, t dbSNP:766696797
246 246 a, g dbSNP:761014607
247 247 c, t dbSNP:773597414
248 248 a, c dbSNP:201794303
253 253 a, g dbSNP:144297311
264 264 a, g dbSNP:774796594
274 274 c, g dbSNP:776267174
288 288 g, t dbSNP:771337378
290 290 c, t dbSNP:542654093
291 291 c, g dbSNP:753152936
294 294 g, t dbSNP:764833663
301 301 a, g dbSNP:150925566
313 313 a, g dbSNP:112230436
314 314 a, g dbSNP:561349857
322 322 c, t dbSNP:770487074
331 331 c, t dbSNP:760587497
334 334 c, t dbSNP:377313136
337 337 c, t dbSNP:771947200
343 343 a, g dbSNP:753258113
349 349 a, c dbSNP:201065285
375 375 c, t dbSNP:760429750
377 377 c, t dbSNP:4988460
383 383 a, g dbSNP:142837047
389 389 c, t dbSNP:542678664
390 390 c, g dbSNP:774401165
392 392 a, c dbSNP:768471285
400 400 c, g, t dbSNP:776549000
402 402 a, g dbSNP:770948248
418 418 c, t dbSNP:747262422
460 460 a, g dbSNP:777904227
462 462 c, t dbSNP:138356138
463 463 a, g dbSNP:748457352
476 476 a, c, g dbSNP:755420364
490 490 c, t dbSNP:141400089
495 495 a, g dbSNP:143373007
497 497 a, g dbSNP:755621601
505 505 c, t dbSNP:750117553
514 514 c, g dbSNP:147095437
523 523 a, c dbSNP:761731319
529 529 g, t dbSNP:104893761
534 534 a, g dbSNP:773983156
535 535 a, g dbSNP:764138369
546 546 a, g dbSNP:762819183
552 552 a, c dbSNP:142046308
553 553 a, g, t dbSNP:104893759
558 558 a, t dbSNP:104893763
560 560 a, g, t dbSNP:773491464
563 563 a, t dbSNP:772252418
568 568 a, g dbSNP:374079879
572 572 a, c dbSNP:758184285
576 576 a, g dbSNP:752457957
577 577 a, c dbSNP:765209272
582 582 a, g dbSNP:759494533
597 597 a, c, g dbSNP:104893756
598 598 a, c dbSNP:761898822
599 599 a, c dbSNP:774499181
606 606 c, t dbSNP:768914485
607 607 a, c dbSNP:771621466
608 608 c, t dbSNP:35677731
609 609 g, t dbSNP:770232339
613 613 a, c dbSNP:200928407
638 638 c, t dbSNP:777210167
639 639 c, t dbSNP:104893754
640 640 a, g dbSNP:104893765
650 650 c, t dbSNP:777330391
657 657 c, g dbSNP:758047644
661 661 g, t dbSNP:747803288
662 662 c, g dbSNP:104893766
672 672 a, g dbSNP:778713773
674 674 a, g dbSNP:754703680
676 676 g, t dbSNP:753790272
684 684 a, g dbSNP:766330498
692 692 a, g dbSNP:757350287
695 695 a, g dbSNP:367874840
698 698 a, c dbSNP:143471841
702 702 c, t dbSNP:104893758
705 705 a, c dbSNP:775812519
710 710 a, g dbSNP:765698999
717 717 a, g dbSNP:759920991
723 723 a, g dbSNP:777062646
736 736 a, c dbSNP:760045491
740 740 g, t dbSNP:777030970
759 759 -, g dbSNP:775878145
763 763 -, ggaaa dbSNP:772390221
768 768 a, c dbSNP:766603627
774 774 c, t dbSNP:761275346
775 775 a, g dbSNP:374853152
776 776 a, c dbSNP:773523196
786 786 a, g dbSNP:771581828
791 791 c, t dbSNP:781346089
792 792 a, g dbSNP:770960302
793 793 a, t dbSNP:768462346
797 797 c, t dbSNP:746893965
801 801 a, g dbSNP:779164965
804 804 g, t dbSNP:1131815
813 813 a, g dbSNP:104893764
817 817 a, g dbSNP:780123391
820 820 a, c dbSNP:756565722
827 827 a, t dbSNP:750719768
832 832 a, g dbSNP:768057198
839 839 a, c dbSNP:762334396
840 840 c, t dbSNP:104893762
856 856 c, t dbSNP:752024682
862 862 a, g dbSNP:763601390
872 872 -, a dbSNP:587776798
873 873 g, t dbSNP:104893760
878 878 g, t dbSNP:762314553
879 879 a, t dbSNP:775273679
882 882 c, g dbSNP:764794342
885 885 a, g dbSNP:553845639
892 892 a, t dbSNP:776545755
896 896 a, g dbSNP:572428799
899 899 -, a dbSNP:587776799
900 900 a, g dbSNP:746947103
902 902 -, ag dbSNP:754512389
903 903 a, g dbSNP:773224032
911 911 c, t dbSNP:768836863
917 917 c, t dbSNP:749321525
918 918 c, t dbSNP:780359925
926 926 a, g dbSNP:756263733
929 929 a, t dbSNP:746045997
936 936 c, t dbSNP:104893755
944 944 a, c dbSNP:377383513
953 953 a, g dbSNP:757553541
970 970 c, g dbSNP:752075780
976 976 a, c dbSNP:371440507
984 984 c, t dbSNP:757994529
986 986 a, t dbSNP:752117643
989 989 a, t dbSNP:764989592
992 992 a, g dbSNP:144079829
996 996 a, g dbSNP:776355574
998 998 a, g dbSNP:144708108
1003 1003 a, g dbSNP:535230308
1008 1008 a, t dbSNP:773347042
1009 1009 c, t dbSNP:771870583
1015 1015 c, t dbSNP:749430698
1023 1023 a, c, g dbSNP:568124971
1034 1034 c, t dbSNP:368569904
1035 1035 a, g dbSNP:781336944
1037 1037 g, t dbSNP:757679981
1050 1050 a, c dbSNP:747372552
1086 1086 a, t dbSNP:775122027
1098 1098 c, t dbSNP:768535274
1133 1133 -, t dbSNP:376540322
1139 1139 -, t dbSNP:368061882
1139 1139 a, t dbSNP:190287993
1140 1140 -, t dbSNP:575761620
1140 1140 aa, tt dbSNP:368653404
1140 1140 a, t dbSNP:4988463
1141 1141 -, t dbSNP:201314981
1141 1141 a, t dbSNP:33936108
1142 1142 a, t dbSNP:552876873
1148 1148 -, a dbSNP:369739158
1177 1177 -, ttat dbSNP:370744962
1185 1185 a, t dbSNP:186969426
1197 1197 g, t dbSNP:527903623
1201 1201 c, t dbSNP:566989622
1214 1214 a, g dbSNP:6792500
1246 1246 a, g dbSNP:771316195
1248 1248 a, g dbSNP:560712991
1256 1256 -, ctct dbSNP:746818570
1258 1258 c, g dbSNP:747415516

Target ORF information:

RefSeq Version NM_000306
Organism Homo sapiens (human)
Definition Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant alpha, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000306

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGAGTTGCC AAGCTTTTAC TTCGGCTGAT ACCTTTATAC CTCTGAATTC TGACGCCTCT 
GCAACTCTGC CTCTGATAAT GCATCACAGT GCTGCCGAGT GTCTACCAGT CTCCAACCAT
GCCACCAATG TGATGTCTAC AGCAACAGGA CTTCATTATT CTGTTCCTTC CTGTCATTAT
GGAAACCAGC CATCAACCTA TGGAGTGATG GCAGGTAGTT TAACCCCTTG TCTTTATAAA
TTTCCTGACC ACACCTTGAG TCATGGATTT CCTCCTATAC ACCAGCCTCT TCTGGCAGAG
GACCCCACAG CTGCTGATTT CAAGCAGGAA CTCAGGCGGA AAAGTAAATT GGTGGAAGAG
CCAATAGACA TGGATTCTCC AGAAATCAGA GAACTTGAAA AGTTTGCCAA TGAATTTAAA
GTGAGACGAA TTAAATTAGG ATACACCCAG ACAAATGTTG GGGAGGCCCT GGCAGCTGTG
CATGGCTCTG AATTCAGTCA AACAACAATC TGCCGATTTG AAAATCTGCA GCTCAGCTTT
AAAAATGCAT GCAAACTGAA AGCAATATTA TCCAAATGGC TGGAGGAAGC TGAGCAAGTA
GGAGCTTTGT ACAATGAAAA AGTGGGAGCA AATGAAAGGA AAAGAAAACG AAGAACAACT
ATAAGCATTG CTGCTAAAGA TGCTCTGGAG AGACACTTTG GAGAACAGAA TAAACCTTCT
TCTCAAGAGA TCATGAGGAT GGCTGAAGAA CTGAATCTGG AGAAAGAAGT AGTAAGAGTT
TGGTTTTGCA ACCGGAGGCA GAGAGAAAAA CGGGTGAAAA CAAGTCTGAA TCAGAGTTTA
TTTTCTATTT CTAAGGAACA TCTTGAGTGC AGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23455
Accession Version NM_001122757.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pituitary-specific positive transcription factor 1 isoform beta
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC130885.3 and KF457648.1. This sequence is a reference standard in the RefSeqGene project. On Jan 17, 2014 this sequence version replaced gi:170650729. Summary: This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (beta), also known as GHF-2 or PIT-2, represents the longer transcript and encodes the longer isoform (beta). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2158188, SAMEA2162895 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAGTTGCC AAGCTTTTAC TTCGGCTGAT ACCTTTATAC CTCTGAATTC TGACGCCTCT 
GCAACTCTGC CTCTGATAAT GCATCACAGT GCTGCCGAGT GTCTACCAGT CTCCAACCAT
GCCACCAATG TGATGTCTAC AGTCCCATCT ATTTTGTCTT TGATCCAAAC TCCTAAATGT
TTGTGCACAC ATTTCTCGGT GACAACGTTG GGAAACACAG CAACAGGACT TCATTATTCT
GTTCCTTCCT GTCATTATGG AAACCAGCCA TCAACCTATG GAGTGATGGC AGGTAGTTTA
ACCCCTTGTC TTTATAAATT TCCTGACCAC ACCTTGAGTC ATGGATTTCC TCCTATACAC
CAGCCTCTTC TGGCAGAGGA CCCCACAGCT GCTGATTTCA AGCAGGAACT CAGGCGGAAA
AGTAAATTGG TGGAAGAGCC AATAGACATG GATTCTCCAG AAATCAGAGA ACTTGAAAAG
TTTGCCAATG AATTTAAAGT GAGACGAATT AAATTAGGAT ACACCCAGAC AAATGTTGGG
GAGGCCCTGG CAGCTGTGCA TGGCTCTGAA TTCAGTCAAA CAACAATCTG CCGATTTGAA
AATCTGCAGC TCAGCTTTAA AAATGCATGC AAACTGAAAG CAATATTATC CAAATGGCTG
GAGGAAGCTG AGCAAGTAGG AGCTTTGTAC AATGAAAAAG TGGGAGCAAA TGAAAGGAAA
AGAAAACGAA GAACAACTAT AAGCATTGCT GCTAAAGATG CTCTGGAGAG ACACTTTGGA
GAACAGAATA AACCTTCTTC TCAAGAGATC ATGAGGATGG CTGAAGAACT GAATCTGGAG
AAAGAAGTAG TAAGAGTTTG GTTTTGCAAC CGGAGGCAGA GAGAAAAACG GGTGAAAACA
AGTCTGAATC AGAGTTTATT TTCTATTTCT AAGGAACATC TTGAGTGCAG ATAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001116229.1
CDS126..1079
Misc Feature(1)78..80(+)
Misc Feature(2)573..797(+)
Misc Feature(3)846..1022(+)
Misc Feature(4)846..1016(+)
Misc Feature(5)852..1004(+)
Exon (1)1..267
Gene:POU1F1
Gene Synonym:
Exon (2)268..417
Gene:POU1F1
Gene Synonym:
Exon (3)418..642
Gene:POU1F1
Gene Synonym:
Exon (4)643..807
Gene:POU1F1
Gene Synonym:
Exon (5)808..868
Gene:POU1F1
Gene Synonym:
Exon (6)869..1340
Gene:POU1F1
Gene Synonym:
Translation
Position Chain Variation Link
40 40 c, t dbSNP:547104320
78 78 c, t dbSNP:771498395
84 84 a, g dbSNP:369089154
88 88 a, g dbSNP:778499770
90 90 c, t dbSNP:754597573
93 93 c, g dbSNP:775811831
94 94 a, g dbSNP:372288270
98 98 c, t dbSNP:756681562
100 100 c, t dbSNP:779842896
102 102 c, t dbSNP:755859410
113 113 c, t dbSNP:368528397
115 115 c, g dbSNP:767428065
120 120 g, t dbSNP:762999659
128 128 c, g dbSNP:752815164
131 131 c, t dbSNP:201156427
133 133 a, g dbSNP:759809037
136 136 c, t dbSNP:1051612
139 139 -, c dbSNP:767075581
139 139 c, t dbSNP:776954429
152 152 c, t dbSNP:35560664
156 156 a, t dbSNP:4988459
157 157 a, c, t dbSNP:773651922
164 164 a, g dbSNP:772631185
165 165 c, g dbSNP:200873915
166 166 c, t dbSNP:773832905
181 181 c, t dbSNP:35182189
188 188 a, c dbSNP:146118849
196 196 c, t dbSNP:104893757
197 197 g, t dbSNP:779611176
206 206 a, g dbSNP:142491010
209 209 a, t dbSNP:755914583
213 213 a, c dbSNP:745659872
215 215 a, c, t dbSNP:543096490
222 222 a, g dbSNP:752767611
225 225 c, t dbSNP:368440919
227 227 c, t dbSNP:374097539
229 229 c, t dbSNP:558209279
242 242 a, c, t dbSNP:370682988
245 245 c, t dbSNP:766696797
246 246 a, g dbSNP:761014607
247 247 c, t dbSNP:773597414
248 248 a, c dbSNP:201794303
253 253 a, g dbSNP:144297311
264 264 a, g dbSNP:774796594
271 271 c, t dbSNP:759254509
281 281 a, g dbSNP:776081778
283 283 a, c dbSNP:770773559
286 286 c, t dbSNP:367977810
295 295 -, ctcctaaa dbSNP:751213951
298 298 c, t dbSNP:374725184
304 304 a, g dbSNP:772106853
305 305 g, t dbSNP:749431428
311 311 c, t dbSNP:780208819
316 316 a, g dbSNP:546702859
322 322 c, t dbSNP:371295598
323 323 c, g dbSNP:189221434
332 332 a, g dbSNP:781210029
333 333 c, t dbSNP:757674048
342 342 a, t dbSNP:35893626
344 344 a, g dbSNP:746709336
352 352 c, g dbSNP:776267174
366 366 g, t dbSNP:771337378
368 368 c, t dbSNP:542654093
369 369 c, g dbSNP:753152936
372 372 g, t dbSNP:764833663
379 379 a, g dbSNP:150925566
391 391 a, g dbSNP:112230436
392 392 a, g dbSNP:561349857
400 400 c, t dbSNP:770487074
409 409 c, t dbSNP:760587497
412 412 c, t dbSNP:377313136
415 415 c, t dbSNP:771947200
421 421 a, g dbSNP:753258113
427 427 a, c dbSNP:201065285
453 453 c, t dbSNP:760429750
455 455 c, t dbSNP:4988460
461 461 a, g dbSNP:142837047
467 467 c, t dbSNP:542678664
468 468 c, g dbSNP:774401165
470 470 a, c dbSNP:768471285
478 478 c, g, t dbSNP:776549000
480 480 a, g dbSNP:770948248
496 496 c, t dbSNP:747262422
538 538 a, g dbSNP:777904227
540 540 c, t dbSNP:138356138
541 541 a, g dbSNP:748457352
554 554 a, c, g dbSNP:755420364
568 568 c, t dbSNP:141400089
573 573 a, g dbSNP:143373007
575 575 a, g dbSNP:755621601
583 583 c, t dbSNP:750117553
592 592 c, g dbSNP:147095437
601 601 a, c dbSNP:761731319
607 607 g, t dbSNP:104893761
612 612 a, g dbSNP:773983156
613 613 a, g dbSNP:764138369
624 624 a, g dbSNP:762819183
630 630 a, c dbSNP:142046308
631 631 a, g, t dbSNP:104893759
636 636 a, t dbSNP:104893763
638 638 a, g, t dbSNP:773491464
641 641 a, t dbSNP:772252418
646 646 a, g dbSNP:374079879
650 650 a, c dbSNP:758184285
654 654 a, g dbSNP:752457957
655 655 a, c dbSNP:765209272
660 660 a, g dbSNP:759494533
675 675 a, c, g dbSNP:104893756
676 676 a, c dbSNP:761898822
677 677 a, c dbSNP:774499181
684 684 c, t dbSNP:768914485
685 685 a, c dbSNP:771621466
686 686 c, t dbSNP:35677731
687 687 g, t dbSNP:770232339
691 691 a, c dbSNP:200928407
716 716 c, t dbSNP:777210167
717 717 c, t dbSNP:104893754
718 718 a, g dbSNP:104893765
728 728 c, t dbSNP:777330391
735 735 c, g dbSNP:758047644
739 739 g, t dbSNP:747803288
740 740 c, g dbSNP:104893766
750 750 a, g dbSNP:778713773
752 752 a, g dbSNP:754703680
754 754 g, t dbSNP:753790272
762 762 a, g dbSNP:766330498
770 770 a, g dbSNP:757350287
773 773 a, g dbSNP:367874840
776 776 a, c dbSNP:143471841
780 780 c, t dbSNP:104893758
783 783 a, c dbSNP:775812519
788 788 a, g dbSNP:765698999
795 795 a, g dbSNP:759920991
801 801 a, g dbSNP:777062646
814 814 a, c dbSNP:760045491
818 818 g, t dbSNP:777030970
837 837 -, g dbSNP:775878145
841 841 -, ggaaa dbSNP:772390221
846 846 a, c dbSNP:766603627
852 852 c, t dbSNP:761275346
853 853 a, g dbSNP:374853152
854 854 a, c dbSNP:773523196
864 864 a, g dbSNP:771581828
869 869 c, t dbSNP:781346089
870 870 a, g dbSNP:770960302
871 871 a, t dbSNP:768462346
875 875 c, t dbSNP:746893965
879 879 a, g dbSNP:779164965
882 882 g, t dbSNP:1131815
891 891 a, g dbSNP:104893764
895 895 a, g dbSNP:780123391
898 898 a, c dbSNP:756565722
905 905 a, t dbSNP:750719768
910 910 a, g dbSNP:768057198
917 917 a, c dbSNP:762334396
918 918 c, t dbSNP:104893762
934 934 c, t dbSNP:752024682
940 940 a, g dbSNP:763601390
950 950 -, a dbSNP:587776798
951 951 g, t dbSNP:104893760
956 956 g, t dbSNP:762314553
957 957 a, t dbSNP:775273679
960 960 c, g dbSNP:764794342
963 963 a, g dbSNP:553845639
970 970 a, t dbSNP:776545755
974 974 a, g dbSNP:572428799
977 977 -, a dbSNP:587776799
978 978 a, g dbSNP:746947103
980 980 -, ag dbSNP:754512389
981 981 a, g dbSNP:773224032
989 989 c, t dbSNP:768836863
995 995 c, t dbSNP:749321525
996 996 c, t dbSNP:780359925
1004 1004 a, g dbSNP:756263733
1007 1007 a, t dbSNP:746045997
1014 1014 c, t dbSNP:104893755
1022 1022 a, c dbSNP:377383513
1031 1031 a, g dbSNP:757553541
1048 1048 c, g dbSNP:752075780
1054 1054 a, c dbSNP:371440507
1062 1062 c, t dbSNP:757994529
1064 1064 a, t dbSNP:752117643
1067 1067 a, t dbSNP:764989592
1070 1070 a, g dbSNP:144079829
1074 1074 a, g dbSNP:776355574
1076 1076 a, g dbSNP:144708108
1081 1081 a, g dbSNP:535230308
1086 1086 a, t dbSNP:773347042
1087 1087 c, t dbSNP:771870583
1093 1093 c, t dbSNP:749430698
1101 1101 a, c, g dbSNP:568124971
1112 1112 c, t dbSNP:368569904
1113 1113 a, g dbSNP:781336944
1115 1115 g, t dbSNP:757679981
1128 1128 a, c dbSNP:747372552
1164 1164 a, t dbSNP:775122027
1176 1176 c, t dbSNP:768535274
1211 1211 -, t dbSNP:376540322
1217 1217 -, t dbSNP:368061882
1217 1217 a, t dbSNP:190287993
1218 1218 -, t dbSNP:575761620
1218 1218 aa, tt dbSNP:368653404
1218 1218 a, t dbSNP:4988463
1219 1219 -, t dbSNP:201314981
1219 1219 a, t dbSNP:33936108
1220 1220 a, t dbSNP:552876873
1226 1226 -, a dbSNP:369739158
1255 1255 -, ttat dbSNP:370744962
1263 1263 a, t dbSNP:186969426
1275 1275 g, t dbSNP:527903623
1279 1279 c, t dbSNP:566989622
1292 1292 a, g dbSNP:6792500
1324 1324 a, g dbSNP:771316195
1326 1326 a, g dbSNP:560712991
1334 1334 -, ctct dbSNP:746818570
1336 1336 c, g dbSNP:747415516

Target ORF information:

RefSeq Version NM_001122757
Organism Homo sapiens (human)
Definition Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant beta, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001122757

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAGTTGCC AAGCTTTTAC TTCGGCTGAT ACCTTTATAC CTCTGAATTC TGACGCCTCT 
GCAACTCTGC CTCTGATAAT GCATCACAGT GCTGCCGAGT GTCTACCAGT CTCCAACCAT
GCCACCAATG TGATGTCTAC AGTCCCATCT ATTTTGTCTT TGATCCAAAC TCCTAAATGT
TTGTGCACAC ATTTCTCGGT GACAACGTTG GGAAACACAG CAACAGGACT TCATTATTCT
GTTCCTTCCT GTCATTATGG AAACCAGCCA TCAACCTATG GAGTGATGGC AGGTAGTTTA
ACCCCTTGTC TTTATAAATT TCCTGACCAC ACCTTGAGTC ATGGATTTCC TCCTATACAC
CAGCCTCTTC TGGCAGAGGA CCCCACAGCT GCTGATTTCA AGCAGGAACT CAGGCGGAAA
AGTAAATTGG TGGAAGAGCC AATAGACATG GATTCTCCAG AAATCAGAGA ACTTGAAAAG
TTTGCCAATG AATTTAAAGT GAGACGAATT AAATTAGGAT ACACCCAGAC AAATGTTGGG
GAGGCCCTGG CAGCTGTGCA TGGCTCTGAA TTCAGTCAAA CAACAATCTG CCGATTTGAA
AATCTGCAGC TCAGCTTTAA AAATGCATGC AAACTGAAAG CAATATTATC CAAATGGCTG
GAGGAAGCTG AGCAAGTAGG AGCTTTGTAC AATGAAAAAG TGGGAGCAAA TGAAAGGAAA
AGAAAACGAA GAACAACTAT AAGCATTGCT GCTAAAGATG CTCTGGAGAG ACACTTTGGA
GAACAGAATA AACCTTCTTC TCAAGAGATC ATGAGGATGG CTGAAGAACT GAATCTGGAG
AAAGAAGTAG TAAGAGTTTG GTTTTGCAAC CGGAGGCAGA GAGAAAAACG GGTGAAAACA
AGTCTGAATC AGAGTTTATT TTCTATTTCT AAGGAACATC TTGAGTGCAG ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.